LOCUS       DAOORU010000001        58873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_579_length_58873_cov_6.038543, whole genome shotgun sequence.
ACCESSION   DAOORU010000001 DAOORU010000000
VERSION     DAOORU010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 58873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58873
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_579_length_58873_cov_6.038543"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..302
                     /locus_tag="PLF20_00005"
     CDS             60..302
                     /locus_tag="PLF20_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79428.1"
                     /translation="MQNEFALEGWMFINYIALHWYYRIYQQLLAKHELNGKFSPKDFI
                     SFLTEIKRVKINDKWYTAEITKKTNDLLKKLELPIT"
     gene            383..3547
                     /locus_tag="PLF20_00010"
     CDS             383..3547
                     /locus_tag="PLF20_00010"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOX79429.1"
                     /translation="MNDKEILIQLYYQKIKASSKELTKKEAFKDLLNRLYSSDKEIRS
                     IIDRITLGAETTVVNIPKKDKLHRGSADTLYNNIIIEFENDLKVSYQHAKEQLAGYLL
                     GQFRSGKGYNFTLIVSDFINWKVLAPDISCLDKLDDLHENELILNEIKSASFVLDESN
                     AEEFYYWIDRFLFKEEKQKATLKSIEESFGYQSSVFIESFRELNNWFNEAKKYGIVQV
                     SFEQWYKFLSIAYGSFDGSESIFLIHTYLSIFAKMLAYAVVSNDDYISDDEMREILNG
                     SIFQSYNISNFVDNDFFHWVNGDRDYLKLKNVFRLIAQEISTFNFSNVDEDILKGVYQ
                     ELIDIDTRHSLGEYYTPDWLCERIVNEYEFKKTDRILDPACGSGSFLRAFIHRIRELN
                     PDATAEEINNQIYGIDIHPLSVQIAKTTLLLALGKGISRARRPIHLNIILANTLLAPE
                     GVQNAFGNDFVMQIDKDKYIINTQIFDNVEVFDAFLDICEELSEQTLNKKDIELSLFE
                     DILKHHTNGGLCSQLVESFYKIYQGVKRVKEKGRNGIWKFILSNLYKPFFLANRFDYV
                     IGNPPWFTYSSIKNKEYQDLLDELAVKYNVKPQKVANYPHLEIAAIFQSYCSSYFLND
                     WGKQAFVLPRSFFSADHHENSRSGIAKGFRLSSIWDLDNVSPLFRIPCCVFFSEKKQK
                     ERIMMSSGLDGKIFVGKLPTHNCNYAIAKNILVETPVKWYYIRQGQSSALSTRKIEMQ
                     RNVNPYKNLFKQGATIVPRAFYFVELDQQMPDDFNDRIISLRTSKFVKADSKDPWKLE
                     FSGRMESRFLFRTAISKSILPFHLYKPDFVVLPLIIQINENQFKKIEIHTADDIMNMG
                     YLNASRWFRNAERIWNINKTEKSKNMSTIGRLNFHNGLTQQNLNAEYLVLYNSSAKDA
                     NALVVNRSSLDLEFICESKSYVYYLNNENEAYYLTALLNSSVPNFLMKDFQTKGLFGP
                     RDIHKKILNIYFPKFDENNKLHLSLAALSKSCHDKTEKYVETNPPNSELTPIKLGKYR
                     VDIKRHLSKEMADIDKIVRKIIKE"
     gene            3995..4447
                     /gene="dtd"
                     /locus_tag="PLF20_00015"
     CDS             3995..4447
                     /gene="dtd"
                     /locus_tag="PLF20_00015"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOX79430.1"
                     /translation="MRVVIQRVSRANVKIDERLISEIGYGLMVLCGFEEGDEVEDLRW
                     IAGKISRLRIFDDEAGMMNLSVVDKAGEMMVISQFTLHASTKKGNRPSYIRALEPVRA
                     KELYQLFVDELVCYSGIGVKTGIFGAYMMIELVNDGPVTIIIDSKMRE"
     gene            complement(4449..4835)
                     /locus_tag="PLF20_00020"
     CDS             complement(4449..4835)
                     /locus_tag="PLF20_00020"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOX79431.1"
                     /translation="MDIIHVPALPLNFEQKKIILAEDTASHYGSGLLEVFATPAMIAF
                     MEKTCYEGIAHYLAPGYSSVGTKVHVEHLRAIRVGVKIHCEARLNTIDGKKLFFEVRV
                     FDHQGVIGQGTHTRYIIDIEKFMLKL"
     gene            complement(4851..5255)
                     /locus_tag="PLF20_00025"
     CDS             complement(4851..5255)
                     /locus_tag="PLF20_00025"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOX79432.1"
                     /translation="MATIETIYLGELRTKATHLQSGSSIITDAPLDNQGRGEAFSPTD
                     LLAAALASCMLTIMGIAARTHGFNLDGTKAEITKIMESNPRRVGEIIIHLYFPPHPFT
                     EKEKQIIRNSAFTCPVYQSLNEQLKKTVHFHF"
     gene            complement(5337..5639)
                     /locus_tag="PLF20_00030"
     CDS             complement(5337..5639)
                     /locus_tag="PLF20_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79433.1"
                     /translation="MKKLVLLFGLGAFIMSSLSASVSLIQRNHSMIVLNDDDPPKKAK
                     TKDAKAETVTPKPIKPCDHPIQGKNTCCDKKEKKASCNEIHKVKQAETKPATPEKK"
     gene            6208..6843
                     /locus_tag="PLF20_00035"
     CDS             6208..6843
                     /locus_tag="PLF20_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HOX79434.1"
                     /translation="MIVLSFTSVPFWAYYRLGSKFPLACQPNYIIVMGAGGMPGPESL
                     LRCHYGTQIAKRYPEAKIIIALPTISEDIYKSHAHKMLEEMVIHGIDSSRFLFETEGT
                     NTREQAKNIAKLKGVGAKDCFLIITSPEHVFRSVKVFRKVGLENCGGLPSFSKELPTE
                     LLLSEDENLQKQLPPDRSPSLRYNMWNYLKLEISVLREYVAIVWYWLNGWI"
     gene            complement(6847..7866)
                     /locus_tag="PLF20_00040"
     CDS             complement(6847..7866)
                     /locus_tag="PLF20_00040"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOX79435.1"
                     /translation="MILVTGSSGLVGSHVLFRLVNEGYRVKALIRSKDRIQSVKRIFQ
                     FYTNKSDELLRQIEWVEGDVLDIGSLEEAFDGVDKVYHCAAVVSFKKADKSKILTTNV
                     KGTLNIVNLCLAKRIIKLAYVSSVAVLGQNPEQKTITEDDLFNEIPKNTTYAMSKFLA
                     EREIWRGIAEGMNAVIVNPSTIIGPGDWQKGSSQLFTEIYHGLKYYTEGVTGYVDVRD
                     VADTLILLMKSEIINERFILNSENLCFKDLFDMMADALGKPRPMVRINKFTAEIAWRL
                     FTLKSLVTGKPSAITAETARSAFVSYYYSNQKFKETFPNYSFIPIRNSIADTAKIFIT
                     EHSLS"
     gene            8018..9301
                     /gene="tyrS"
                     /locus_tag="PLF20_00045"
     CDS             8018..9301
                     /gene="tyrS"
                     /locus_tag="PLF20_00045"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOX79436.1"
                     /translation="MFVKNFVEELRWRVMVHDMTPGTEEYLMQGPSYGYIGFDPTADS
                     LHIGHLVQIMTLVHFQQCGHKPIALVGGATGMVGDPSGRSEERNLLSREQLEYNLVGI
                     KKQLARFIDFETKVNPATMVNNYDWFKGYEFLDFIRDVGKHITVNYMLAKDSVQKRLD
                     AGMSFTEFSYQLVQAYDFYWLFNNMDCRLQMGGSDQWGNIVTGTELIRRKDGGTAYAL
                     TTPLLKKSDGSKFGKTAAGALWLDAEKTSPYAFYQYWINTSDEDAIDYIKIFTTLSKE
                     EIDALSEEHWKTPHLRILQKALAREITIRVHSENDYQMAVEVSEILFGKGTVESLMQL
                     DEKTLLNCFDGVPQFHVSKTELETGIPAIDVLAVKTQIFPSKAEARRMMKDGAVLLNK
                     QKLSEDTFIDNSMLLQNKYLLIQKGKKNYFLVIAE"
     gene            complement(9490..9572)
                     /locus_tag="PLF20_00050"
     tRNA            complement(9490..9572)
                     /locus_tag="PLF20_00050"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9536..9538),aa:Leu,seq:caa)
     gene            9829..10773
                     /locus_tag="PLF20_00055"
     CDS             9829..10773
                     /locus_tag="PLF20_00055"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOX79437.1"
                     /translation="MARNKVKIFSGRSSKYLAEEIAKVYGDELGKVNLTTFSDGEFQV
                     SFEQTVRGNDVYIVQSTFSPVDNLFELLMMIDAAKRASAERIVAVVPYYGFARQDRKD
                     KPRVAIAAKLVADLLSTAGVGRIITMDLHSDQIQGFFNVPVDHLYASTIFVPFIKKLK
                     LPNSVMASPDTGGAKRAAAYAKFLNTELVICFKQRIKPNSIGKMTIIGEVEGKDVILV
                     DDIIDTAGTVCKAADLMMEHGANSVRVFATHAVLSGNALENIHKSSLIEVYVTDTLPV
                     KETEKIKVLSIAPLLADVIDRVHRNKPISSHFDFTTIL"
     gene            10862..11431
                     /locus_tag="PLF20_00060"
     CDS             10862..11431
                     /locus_tag="PLF20_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOX79438.1"
                     /translation="MKTISMSGSLRENVGKKDAKKLRKNGLVPANIYGGANQFLISIE
                     EKHLQKVVFTPEVYLIELDINGTKRQTIIQEVQFHPVTDRILHVDFLEVLPGKPITLK
                     IPIKITGNSIGVLKGGKLQKKFRLLKVKGLASDIPDHITLDVSDLDINHSIKVNDLKY
                     EKLTFLDPASAIVVAVIPTRAVKEGTPQA"
     gene            11538..12125
                     /gene="pth"
                     /locus_tag="PLF20_00065"
     CDS             11538..12125
                     /gene="pth"
                     /locus_tag="PLF20_00065"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOX79439.1"
                     /translation="MKYLIVGLGNPGSEYANDRHNVGFLVMDALAIKTNSRFSTERYA
                     DVAKVKYRGHILVLIKPNTYMNLSGNAVRYWLEKEKISLQHLLIVLDDLDLPQGQIRL
                     RAKGSGGSHNGLNHIIQVLDTEDFPRLRIGIGSDFPKGMQVDYVLGQWSKEEEKIMKP
                     AILDAADAILTFVHLGIERAMNIVNTKKNSLTDDN"
     gene            12628..13032
                     /gene="mce"
                     /locus_tag="PLF20_00070"
     CDS             12628..13032
                     /gene="mce"
                     /locus_tag="PLF20_00070"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOX79440.1"
                     /translation="MQISHIEHIGIAVRSIEESLPYYQEVLGLKCYGIEEVKDQKVRT
                     AFFQAGQTKLELLESTDPDGAIGKFIEKRGEGIHHIAFAVKEIESALEEARSKGIRLI
                     DESPRLGAEGLHIAFLHPKSTHGVLTELCENK"
     gene            13053..14612
                     /locus_tag="PLF20_00075"
     CDS             13053..14612
                     /locus_tag="PLF20_00075"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOX79441.1"
                     /translation="MAIEDKLLELLEKRQKASLGGGQKRIDAQHAKGKLTARERIDLL
                     LDDGSFEEFDMFVTHRCTDFGMQDQLYLSDGVVTGHGTIDGRVVYVFSQDFTVFGGSL
                     SESYAKKICKIMDQAMKVGAPIIGLNDSGGARIQEGVLSLAGYAEIFERNILASGLIP
                     QISAIFGPCAGGAVYSPALTDFIIMSQKTSYMFVTGPKVVKTVTGEVVTEEELGGAMI
                     HGAKSGVAHFVAEDEREGILLIRKLLSYLPQNNLEDPPVIPCNDPIDRLDDSLNYFIP
                     DNPNKPYDVKTIIYSIIDENEFLEVQRHYAPNIVTGFSRFDGMTVGIVANQPNYLAGV
                     LDINASRKAARFVRFCDAFNIPLLTLVDVPGFLPGTAQEYGGIIIHGAKLLYAFGEAT
                     VPKVTIILRKAYGGAYDVMSSKHLRGDINYAWPTAEIAVMGPKGAIEILYNKKIQEIA
                     DPAEREDFILNAEKEYKDKFANPYNAARYGYIDDIIEPRNTRFRVIRAFQALATKKDT
                     NPPKKHSNIPL"
     gene            14636..15517
                     /locus_tag="PLF20_00080"
     CDS             14636..15517
                     /locus_tag="PLF20_00080"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HOX79442.1"
                     /translation="MKHFRFFIIIASILPLIGFSQGVNSLKINEILVINDSNYIDDFG
                     ERNGWIEIFNANYNYVDMGGMYLTNDLSNPTKYYIPKGDPRTVIPPRGFIVFYANNKP
                     TRGIMHLNFELKGSKVVAIFSSDGKTLIDKLDIPEEQHPDVTYGRLRDGGPELGYLEK
                     TTPKASNVTEQVATPAEIFGEMDPHGIAITIIAMSVVFVSLALLYILFKNTSKIYSLD
                     LSSIFKRKKGPQKEEEKAKIGESIPGEVAAAIAMALYQYHAQIHDVEKTVLTIRRVSR
                     TYSPWSSKIYGLRKSPR"
     gene            15559..16002
                     /locus_tag="PLF20_00085"
     CDS             15559..16002
                     /locus_tag="PLF20_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOX79443.1"
                     /translation="MKRFKFKINGNTYETEIIDIEDNIATIEINGSRYTVEVDQTLTP
                     VKTPKLMRTPAVPSTDIHPAVSKTSAPIGPKGTGYIKSPLPGVILDILVKEGDYVKIG
                     QKVIVLEAMKMENNIESDKEGRVVSIKKQKGDAVLEGDILIVIGE"
     gene            16004..17167
                     /locus_tag="PLF20_00090"
     CDS             16004..17167
                     /locus_tag="PLF20_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOX79444.1"
                     /translation="MAENEGFLKFIVDHLEQFFSYTAFANITVGHIIMICVGLFFIFL
                     AITKEYEPLLLVPIGFGILIGNIPFYPGLKVGIYEDGSVLNYLYFGVVQGIYPPLIFL
                     GIGAMTDFSALLSNPKLILIGAAAQFGIFGAYIAALALGFAPNEAGAIGIIGGADGPT
                     AIFLTSKLAPELLGAIAISAYSYMALVPVIQPPIMRLLTTPKERVIRMKPPRVVSKNE
                     KILFPIIGLLLTCFIVPSGLPLLGMLFFGNLLKESGVTKRLADTAKGPLIDIVTILIG
                     LTVGASTQATTFLTPKCIGIFALGAFSFMVATAAGVLFVKFLNLFLKPEDKINPLIGN
                     SGVSAVPDSARLSQVIGLEYDKTNYLLMHAMGPNVAGVIGSAVAAGILLSFLY"
     gene            17265..17880
                     /gene="udk"
                     /locus_tag="PLF20_00095"
                     /pseudo
     CDS             17265..17880
                     /gene="udk"
                     /locus_tag="PLF20_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802905.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
     gene            complement(18084..18992)
                     /locus_tag="PLF20_00100"
     CDS             complement(18084..18992)
                     /locus_tag="PLF20_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79445.1"
                     /translation="MENTNDQKSNKGIIIAGVVILLLAIFFGWKWVSTQSEISQIKKI
                     AEEEKAILRHELDSVMAEHERIKAAYGELADSLRVKDSIIQASALEIKKLLDTKWEYI
                     KVSRKLDQLRLVAQSYLRQMDSLYTVNRQLQEENLQMKEEIIAEQQKAKQLVQEKELL
                     TTKIKQGSALSISSLTAETLRLRSGGKEEFTDKGKRIDKIRVCFIIAENRIAQPGTRT
                     FYVRIADPNGNILTRSRGDEYSFTYQGEQLQYSTMANIDYQNKAVKMCADYNKLPLQE
                     KFISGTYQVAVYLGDDLLGETSFTVK"
     gene            complement(19147..20337)
                     /gene="kbl"
                     /locus_tag="PLF20_00105"
     CDS             complement(19147..20337)
                     /gene="kbl"
                     /locus_tag="PLF20_00105"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOX79446.1"
                     /translation="MYKEFQEFLSKELSAIEEAGLAKNERIIVSPQGAEITLNTGQKV
                     LNFCANNYLGLSNNPKLIEAAKAAMDTHGFGMSSVRFICGTTDLHKKLEAKIAEYFGT
                     EDTILYAACFDANGGVFEPLLSEEDAIISDALNHASIIDGVRLCKAVRYRYANADMKE
                     LEERLKDSQKQRFRLIVTDGVFSMDGNVAPIDKIYELANKYDAMVMIDESHSAGVVGQ
                     TGRGVTELFKLEGKIEIITGTLGKAFGGAIGGFTTGKKEIISMLRQRSRPYLFSNSLP
                     PMVVAAGIRMFDMMKETNDLQDKLHKNTAYFSQRMSEIGFDIKPTQSAIIAVMLYDAK
                     LAQDFAARLLDEGIYVTGFSYPVVPKGQARVRVQISAAHEKEHLDKAIAAFTKVGKEM
                     GIIK"
     gene            20556..21518
                     /locus_tag="PLF20_00110"
     CDS             20556..21518
                     /locus_tag="PLF20_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOX79447.1"
                     /translation="MKKILVIGSTGQIGSELTLALRKEFGGENVIAGIRKTAPSKQIL
                     ETGPCEVVDATIREQMERAVEKHDIDTIVHMAAILSAVGEQNPMMAWNVNMNGLINVL
                     ECARDRKMDRVLVPSSIAVFGPGTPLDNTPQETILRPTTMYGITKVAGELLGDYYVLK
                     YGLDVRGLRYPGIISHETLPGGGTTDYAVAIYYEAVKNKKYTCFVREDTRLPMMYMPD
                     CLKATISLLKADFNKLRHHCDFNVSAMSFSVKELADSIKKYIPEFEVSYEPDFRQKIA
                     DSWPNSIDDSYAREEWGWKPDYDLDAMTRDMLSALQRKHQQGFI"
     gene            21799..22461
                     /locus_tag="PLF20_00115"
     CDS             21799..22461
                     /locus_tag="PLF20_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOX79448.1"
                     /translation="MLCFDVHTHHFRADNRSIFNLILKEDGSMIPEMYFSSGIHPWYI
                     TDKWKKILEDHFRENYKQRVAIGETGIDMLKGTDLKLQELVFMAHIEWALNYSMPLVI
                     HSVRSSQRIIEILRAMNFQFPVVIHGFRQSNELAKKFLSEGYYLSLGVYVNYAGQKLT
                     EAIKYIPLDRILIESDDFDGDCCEIYHQIAHIKGISADELDNVVLMNVKNCFRIIDGK
                     LA"
     gene            22445..23176
                     /locus_tag="PLF20_00120"
     CDS             22445..23176
                     /locus_tag="PLF20_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOX79449.1"
                     /translation="MENWLERTELLLGKEKLEALKRSHVLVAGLGGVGAYLAESLCRA
                     GLGELTLADSDIVKPSNRNRQLIALSSTEGRLKAEAMAERLRDINSEIHLHIITQYLK
                     DDVLKDVVCRPYDYVADALDTLSPKLFLLIHSVNYGHKIISSMGAGSKIHPERIMVAD
                     ISETNTCPLALLLRKRLKKHGISKGIKAVFSYEAVMEGSRKYVNDELNKKTIVGTISY
                     MPAIFGLFMASVIIRDLTGTLPVPY"
     gene            23320..23392
                     /locus_tag="PLF20_00125"
     tRNA            23320..23392
                     /locus_tag="PLF20_00125"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23353..23355,aa:Gln,seq:ctg)
     gene            complement(23796..25277)
                     /locus_tag="PLF20_00130"
     CDS             complement(23796..25277)
                     /locus_tag="PLF20_00130"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX79450.1"
                     /translation="MKQIFQLSVFVLLATFVFGQQVPREMVILEIGTGTWCTYCPGAA
                     MGADDLLANGCMVAVVENHNGDPFANQYSNARNSFYGITGFPTAIFDGISKVVGGNHS
                     QSMYPTYLPRYNQRIAIPCDFTMDMQITNSGLDYTAVITVTKVAPNTATGLKLHFFVT
                     QSHISYNWQGQNHVNFVNRLMVPDQNGTAIDFSGGDVVIVTLNFSLDPTCPIEDVEFV
                     AGIQAQNKEFLQGTKQAAIDLRVDFTANDTVIPINQPVIFTNNTTGGYIGTPETYQWF
                     FPGATPDTSSLKNPTVTYTECGSHNVKLIVYRGGQIDSLERQAYVQVGPLVNITASPS
                     DTSFWPFNPIVLDATIDDPQATYLWQPGGETTSSITVSFDQYGLGEHTFTVTVNSSGC
                     EITKSHTIYFYGVEGISNNKNHHLDIFPNPASSSLHICVEKPEVYNIYIKDLTGKTII
                     SKPSENFASGNDYILDIKNLSRGIYLLQLVNESSSYTQKLIVR"
     gene            complement(25408..29055)
                     /locus_tag="PLF20_00135"
     CDS             complement(25408..29055)
                     /locus_tag="PLF20_00135"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOX79451.1"
                     /translation="MMKTKFYSLTVYATLIFFMLMQSVGVLAQTQTITLQNSSTKQGF
                     NLKESKSSAVEVQYVVNEFYLEDFTLNGEQMKSVLLSGVFLPNDEGAPNLAGESRFIA
                     IPQGATPVLDIKSVEIEKFQNVEVGPAPRIPLESDDSPLHYEKNPAIYSVNAFYPAQP
                     IKLSEVSKIRGVDVVLLGITPFQYNPVTKELIVYKNIDVEIRFEGGNSQFGENAYRNP
                     YFDAILSDNLLNYSSLSHIDYAARYASYYAPTEDDECEYIIICPDGPEFQTWADSIRK
                     FRTEQGILTKVFTLTQIGGNTTTAIETFINNAYNNWSIKPIACLLLGDYGTNATNSII
                     SPIYNNYCVSDNIYADVDGDMMPDVIFSRITARNTTELQVMVTKVLNYERNPPTDTTF
                     YNRPITALGWQTERWFQICSEVVGGFFKNKLGKNPVRQNAIYQGNPNVDPWSTATNTN
                     TVLNYFGPNGQGYIPATPQELGGWTGGSATGINNAINNGAFIIQHRDHGMETGWGEPS
                     YTNSNINALSNTNLTFVMTINCLTGKYNWGSECFAEKFHRHTKNGLNAGALGLLAPSE
                     TSYSFVNDTYVWGVYDNWWTDFMPSYASTPEPRGVLPSFGNAAGKYFLRQSSWPYNTN
                     NKAVTYYLFHHHGESWSVLYTQMPQHINITYDSVIYQTNTTFTVEATPGAQVALTLDG
                     QIVGVQTANITPIVFNLPPLTAGQKLVLVATKQNYFRYRHEMTVAIDQLLANFTANIT
                     SGCEGLSVDFTDLSSGHPTSWLWTFEGGTPSTSTLQNPTGIVYATQGSYPVTLTVTKG
                     TESHTTTLENFITAWAEPTAPIAHDTSACYAMPVPDLIAEGENIRWYSDSLLTNQVYS
                     GNTFPTGITQIGTFNYYVTQSAGTCEGPATMVTLTISDYPIVTLTPFETVCVNTDPFE
                     LTGGNPEGGLWQGIGVDTNYFNPSTAGAGEHPLSYTYTNEFGCSTTISQPITVMDLPE
                     VSLAPFTPLCDNTDPIELTGGVPEGGIYSGNGVENGFFYPSNIGAGETEILYSYTDNT
                     TGCSNFASQSISVKASPIVLFGGDTTICHNHVITLDATNENSTYLWSTGETTPAIIVD
                     STGVGIGGVKNISVTVNNSDGCIASANINITFKDCTGIEELASSLGISIYPNPNQGNF
                     TIEFHAKQPTSVNMRILNLFAQQVFALDNLKINTQTLQKINLDQLPDGIYILVIETNG
                     RTFTQKFVINK"
     gene            complement(29087..31153)
                     /locus_tag="PLF20_00140"
     CDS             complement(29087..31153)
                     /locus_tag="PLF20_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOX79452.1"
                     /translation="MRKFYLILAECLMFSMVAKTQVIEHSYSWNNPIIKDISGYSSLF
                     LAGCQIAGEAGAPGLPYQAVKLLLPPAHEADSMEVEYSQPFEIHLASSILPMQHAKPY
                     SLAGNSKWLQNEKIYSSSKPYPNSKQGKLSTHFFKGLSIALSTLTPVEYFPKEQKVIV
                     YQHAKVKIFCHETHKARKALENLYPVSFSDKDLKNLIQNASEIHQYASDKKLNNPYDL
                     LILTKESFTEYFFPLASLYEQNGIAVRIDTLENVLTNTAGQDDQEKIRNFIIAQYQQN
                     GISYVLLGGDVEIFPARGFYCKVFSSSIYEDNNIPSDLYYSALDGTWNDDGDDLWGEI
                     GEDDLLPEVAVARLPFSTTTELQNMLHKIISYQSNPIPNELEKPLMAGELLYNNPLTF
                     GEDYLKQLIGYHEDNGYITNGIPEYQEIDSLYESHLGYWSKYDLLQKINTGKSFIHHA
                     GHANWDYAMKLSSSDINNQNFSQVNGIDHNYTLVYTHGCICGAFDYSDCIAEEMLKIE
                     NFAVAGAFNSRYGWFNEGQTEGPSLHLHREFVNALYTEGYDRIGATHQYSRIKTAPWV
                     NAPGQWEEGALRWCFYDCNILGDPAMKIWTSNLTSIPYARNNSNNFNIVYSPVNQFIE
                     ITSEESSSFQLQLIDFTGRIILTKDNRNQKNIKLDTQTVKNGLYLLHINSNSFHLTKK
                     ILIYNL"
     gene            complement(31312..32361)
                     /locus_tag="PLF20_00145"
     CDS             complement(31312..32361)
                     /locus_tag="PLF20_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX79453.1"
                     /translation="MKTKILLMSLVLILSTRLIMGQITITYHDFPPIPYSTWEYVPTT
                     LENTDISPNGANQIWDLSGLIAADSTPIQISDLANLPFAGQYPEGNLVFQKNNNYYIL
                     SRTPDKIGIIGAVMPMDTALLLSIIYITPELIYTYPYTMGSIVESHPQSLTKIPYNDT
                     ISGYFVDSLAINIRASVKHEVVGWGIMKLPYADIKVLKTLTNKTENTTILAYIMGTWF
                     EVDSSINVYNSIEFQMHNLLYPLVTVELDTFNQPVNLNWIRVDPSLHTRNYPDFSFRY
                     FPNPVQNFVIFESDRNISRIELISMDGKIVKLIEVVPSPQIKIKLNDLPTGLYVFKLY
                     DSEELIRLGKLIKRQ"
     gene            32746..33795
                     /gene="queA"
                     /locus_tag="PLF20_00150"
     CDS             32746..33795
                     /gene="queA"
                     /locus_tag="PLF20_00150"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOX79454.1"
                     /translation="MKLSQFKFYLPQELIANYPADDPDAARMMVLNRKTQTIEHKQFK
                     DVIDYFDEGDVFVINNTKVFPARLYGEKEKTGAKIEVFLLRELNRETRLWDVLVDPAR
                     KIRIGNKLYFGKNDELVAEVIDNTTSRGRTIRFLYDGPYEKFRKTLMQLGETPLPKIH
                     QRPVETEDAKRYQTIYAKYEGAVAAPTAGMHFTRNLMKRMEIKGIRFAEITLHTGVGN
                     FREIEVEDLTKHKMDSEEMFIEEEAAKIINEAKLSHRKVCAVGTTSLKALETAATING
                     KIKPYRGWSNKFIFPPYEFSVPDAMISNFHLPLSPMLMVVCAFGGYDFVMKAYKEAIE
                     KKYKFFTYGNAMLII"
     gene            33834..34529
                     /locus_tag="PLF20_00155"
     CDS             33834..34529
                     /locus_tag="PLF20_00155"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF001186.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOX79455.1"
                     /translation="MMEKYAIIVAGGSGSRMKSVTPKQFALLAGKPVLLYSIEAFLKA
                     FEDIRIVIVIQQGMEAFWFTMKKEYQLSERILIVHGGTERFHSVKNGLEKVPDDVLVG
                     IHDAARPLIDAELIGKTFQIAEKKKNCIPAIFAHDSVRILDDVEISRPVPRERVQLIQ
                     TPQVFYSTPLKKAYRTSFHEQFTDDASIWEFAGNKVEFCEGSPFNLKITIPSDFALAE
                     ALLMARQKEAGSF"
     gene            complement(34574..37354)
                     /gene="leuS"
                     /locus_tag="PLF20_00160"
     CDS             complement(34574..37354)
                     /gene="leuS"
                     /locus_tag="PLF20_00160"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOX79456.1"
                     /translation="MEYNFREIELKWQKYWSNNKTFKTENASSKPKFYVLDMFPYPSG
                     AGLHIGHPLGYIASDIYARYMRLKGYNVLHPMGYDAFGLPAEQYAIQTGTHPEITTRK
                     NIERYRKQLDRLGFSFDWDREIRTCDPSYYKWTQWTFIQLFNSWYDKKLDKACPISEL
                     MEYFSLNGNEGIEAVSGKVPSFSAQYWNSATEKTRSEILMEYRLAYQSETIVNWCSEL
                     GTVLANDEVSDGLSVRGGYPVERVKMKQWLLRITAYAERLLEGLEKIDWSDSIKDIQR
                     NWIGRSEGCTIFFKLADYEDAIEIFTTRPDTIFGATFMVLSPEHPFAEKITTTSHLYD
                     VSSYITWAKNRSERERMAEVKRVTGVFTGAYAINPFNNKKIPVWIADYVLAGYGTGAI
                     MAVPAHDSRDFIFARHFQLPIIQVVKKPNEEILDINLCDSSYDAKEGIMINSGFMNGM
                     DVNDAIKAAVAKVEEMGIGHRTISYRLRDAIFSRQRYWGEPFPVYYKDGIPYTLPENE
                     LPLELPLIDAFLPTTNGEPPLARAKNWNYKGYPLETNTMPGFAGSSGYYFRYMDPHND
                     KAYFSHEAVNYWRNVDLYIGGSEHATGHLIYSRFWCKFLYDIGLAVEDEPFQKLINQG
                     MIQGRSSIVYRIKGTNTFVSLSLKDIYDVQPIHVDIHLVDNDILDVEAFRKWRPDLTD
                     ADFILEDGKYVCGYEIEKMSKSLHNVQNPDDLIEKYGADTLRIYEMFLGPLEQSKPWN
                     TMGIEGVFRFLRKFWKLFHDNHNHFYLSDSPADEKELKILHRTIKKVREDIERYSFNT
                     VVSAFMIAINELTDLKCNKRQILEPLIILISPYAPHIAEELWEMSGHNESITYAHLPD
                     YKGEYLMETSFDYPVSFNGKVRFNISLPLDLNNDEIEQAIINNEKTAYYLQGNKPKKI
                     IVVPGKIINVVY"
     gene            complement(37784..38038)
                     /locus_tag="PLF20_00165"
     CDS             complement(37784..38038)
                     /locus_tag="PLF20_00165"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOX79457.1"
                     /translation="MENLSSIFSSISFGLIAGISIGPTFFLILDHTITQSRTQGFLSA
                     AGVILSDVLIFILAGIGVAQFIEQDNVFFTSDWEGLSCWL"
     gene            38147..39043
                     /locus_tag="PLF20_00170"
     CDS             38147..39043
                     /locus_tag="PLF20_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOX79458.1"
                     /translation="MKKPQHIIWCIGLLLFLLSSLKAQIPSQGFSFVFMTDIHLTRDD
                     YAVEGFRKALAVASDLKPDFIITGGDLVMDALDCDYQHAIDQYDLYLGTISNSPIPFY
                     NAMGNHEIWGWQNKHLRQEEWEDYGKNLFKRRIGPPNYSFDHKGWHFVVINGIEYNDS
                     TSYVGWASDETLDFIQNDLKNVPVGMPIILITHIPLITLEAQWEKGALACNHPMEVIG
                     NSKEVLECFPAHSLRLVLQGHLHYYEKMEIDGIIFITGGAVCGAWWKGNYRVTDEGFV
                     LIRINGEYCQTQYIDYGWEAMR"
     gene            complement(39094..39606)
                     /locus_tag="PLF20_00175"
     CDS             complement(39094..39606)
                     /locus_tag="PLF20_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79459.1"
                     /translation="MEKKYKIWILVLSSVIIFLIVAFITLYARTQRIITGSAPIERPV
                     GQGRGMVIRYLQLDENQTNQYDQIRRQYNVQIASLRDSMLIIRKKMIQELKSDTPDTI
                     KLNYLALEIGRLEGEINMALNQHLLKLKRICRPEQCILLDSLYEGMLNTSKLHQGMKQ
                     GNRYRHGKNP"
     gene            complement(39641..40090)
                     /locus_tag="PLF20_00180"
     CDS             complement(39641..40090)
                     /locus_tag="PLF20_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79460.1"
                     /translation="MKIDCQNFNKWYPRYKSKRLSQQEIISSKQHLLKCTNCSTLAEE
                     IESAVESKVNFEPDPYFFTRFQARLNTQDINRSSTSWIFQSKPRNIFLYGFSIIFAVL
                     MGYYTGRILLPTLPTDELTLQEVLVMEDGIANEPLGLEMIFEESDLE"
     gene            complement(40087..40635)
                     /locus_tag="PLF20_00185"
     CDS             complement(40087..40635)
                     /locus_tag="PLF20_00185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOX79461.1"
                     /translation="MQENNQNAFRILVENFEPMIRSIGYQLFMQEEEVEDFTQEVFMK
                     IHLGISGFRKKSSLSTWIYRIALNHGLNSKRKNSAIPFSHTSHKIYAQALESENNILH
                     PRTFNHFVEKKETRKILNHALNKLPKNQRIAFILTHIEDLSYSEVAEIMEKTPKAIDS
                     LTQRAKNNLRKYLAKYYKLLKP"
     gene            complement(40821..41201)
                     /locus_tag="PLF20_00190"
     CDS             complement(40821..41201)
                     /locus_tag="PLF20_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79462.1"
                     /translation="MKSKMIILCVTMMSLFPIAIMAQTTSKNQSQYKNIGSTPLTSPR
                     FLDQNKDGICDNWPSRAWIQGRGAFIDKNGDGVCDNYGNRTGIRMNRYFVDANNDGVC
                     DNYPSRFSGKRGGHKGYKATQLSR"
     gene            complement(41332..41405)
                     /locus_tag="PLF20_00195"
     tRNA            complement(41332..41405)
                     /locus_tag="PLF20_00195"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41369..41371),aa:Ala,seq:ggc)
     gene            41532..42212
                     /gene="rsmI"
                     /locus_tag="PLF20_00200"
     CDS             41532..42212
                     /gene="rsmI"
                     /locus_tag="PLF20_00200"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006256326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOX79463.1"
                     /translation="MSKLYLVPTPIGNLEDITLRALRILKEVNFILAEDTRSSRILLQ
                     HYQIQTPLVSHHKFNEHQSLRKVIERLRNGEMAALISDAGTPAISDPGFLLVRECIRE
                     NISIECLPGPVAFIPALVESGLPCDRFYFEGFLPVKKGRKTRMARMAQRDETTIVYES
                     PYRIVRTLKELSEILGMERKASISREISKIFHETIRGTFLELIEWAKSKPLKGEFVIV
                     IGGTKDEN"
     gene            42202..43002
                     /locus_tag="PLF20_00205"
     CDS             42202..43002
                     /locus_tag="PLF20_00205"
                     /inference="COORDINATES: protein motif:HMM:NF020442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FRG domain-containing protein"
                     /protein_id="HOX79464.1"
                     /translation="MKTDLIAKNWVHLQDLLFRDTWDKDLKRYRSAYVYRGLSDKNYQ
                     LVSSLIRLGGPYDRLERHLLRNFIKYARRGSTGESLWNWLVLAQHHGLPTRLIDWTYS
                     PLVALHFATANPLKYKDDGVIWCVNYVKANHFLPQSLLDIILEEGSNVFTPYMLDKVC
                     NSLSKLSELQSEPFNIFLEPPSLDERIVNQHALSSLMSTAKAHLGVWLQNHLDIYFRI
                     IIPAELKWEIRDKLDQSNITERVLFPGLDGLSLWLKRNYSPKDIPFDI"
     gene            43015..43467
                     /locus_tag="PLF20_00210"
     CDS             43015..43467
                     /locus_tag="PLF20_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOX79465.1"
                     /translation="MSYTYEYPRPCVTVDVALFRKHHDKEEILLIQRKNPPFEGMWAL
                     PGGFINMDENLSEAAFRELKEETGLTPRTLFQFRTYGDVYRDPRHRTITIVYYGFIDF
                     EVSQPLSGDDAADAAWFSIQKLPPLAFDHTQIINELIEYLISNHQIVL"
     gene            43464..44522
                     /locus_tag="PLF20_00215"
     CDS             43464..44522
                     /locus_tag="PLF20_00215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX79466.1"
                     /translation="MKKLIFPFVLFLTFFKLHAQVVLHDSLWPEPGTFIYMRIVDDPA
                     SMHFTHSGENVNWDFTSLVSSDQDTIAYLDPSTTPYFGSFPQSNLALGMSPTEMAYLQ
                     NDEFDSQLIGINGDPGTGTPLTVPLHPSLHLFHFPYTYGDSIFSTARAIIRGTGAMFG
                     QPALDSMKLVSTIITHRKVMAWGLLLLPCSGFPETLLEKGIITRIDSAWTKVPFFGWI
                     PVPGFPMITVDTMYNWFTANSIHPYAQLVVDQSGTPYSASYYSGNLEAIDEYSHSQNF
                     PFVVSPNPVESEVSLFSTQTGWSGKLFDYTGRLLKDDIKVRIGTTRVDVSGLKTGVYL
                     LVLKNNRGEVKTIKIQKR"
     gene            complement(44524..46242)
                     /gene="lysS"
                     /locus_tag="PLF20_00220"
     CDS             complement(44524..46242)
                     /gene="lysS"
                     /locus_tag="PLF20_00220"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOX79467.1"
                     /translation="MALSEQEIIRRQALQELMNLGIDPYPTGVFPVNAMANEILTNYN
                     PELNNYQEVCIAGRIMSRRVMGAASFLELQDSTERIQLYVKRDDLCLNEDKTFYNIVF
                     KKLLDIGDIIWVKGFVFITQMGEITIHVKELELLAKSLRPLPIVKEKDDKVYDAFQNP
                     EQRYRQRYLDLIVNPHVRQIFIKRTQMVNSMRDFLNWKGYLEVETPILQPLYGGAAAR
                     PFKTYHNALDATLYLRIANELYLKRLVVGGFDGVYEFSKDFRNEGMDRFHNPEFTQME
                     LYVAYKDYYWMMDLVEEMVEKIALDLHSSTQIQVGDHVINFKRPWKRFTMTGIIQEFT
                     GLDISDMDETQLRQTATRLNIEITDAMGKGKIIDEIFGEKCEPYLIQPTFIMDYPIEM
                     SPLAHKHRSKTGLVERFEMICNGKEICNAFSELNDPIDQRQRFEAQLELRRRGDEESM
                     VLDEDFLRALEYGMPPTAGLGIGIDRLAMIMTNSSSIQEVILFPQMRPEKSQEVAPPS
                     DLEDTGIPREWIPLVIQAGYTSKEALSQANPGSLFNQLGGLRKKNKIDIPMPSLDEVK
                     QWIANC"
     gene            complement(46576..47805)
                     /locus_tag="PLF20_00225"
     CDS             complement(46576..47805)
                     /locus_tag="PLF20_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOX79468.1"
                     /translation="MSPVGSFESLMAAIQGGANAVYFGAGKHNMRSRSSVNFTLSDLN
                     DISRICKEKGIRSYLTLNSIIYDEELQEMQNIIDTARESGVSAIIASDPAVINYAVEK
                     GIEVHLSTQCNVTNIEAVKWYSRYADVVVLARELNLQQIKQIVDAIKTYDIRGPSGDL
                     VAIEVFVHGALCMAISGKCYLSLHTMNSSANKGACLQICRRRYDVTDKETGTVLEVDN
                     EYIMSPKDLCTIGILDQILAAGVKILKIEGRGRSPEYVKTVTQVYHEGVEAVLNKTFT
                     QEKVAKWTERLRTVYNRGFWEGYYLGRELGEWTQQHGSQATIKKQYLGKVLNYFSKLG
                     VAEVKLETNDLKIGDKYSINGPTSGVVEGTIEEIRLEYDKIPLAPKGRICSFKVSAPV
                     RRGDKVFLLIENPQSLI"
     gene            47974..48306
                     /locus_tag="PLF20_00230"
     CDS             47974..48306
                     /locus_tag="PLF20_00230"
                     /inference="COORDINATES: protein motif:HMM:NF039670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6150 family protein"
                     /protein_id="HOX79469.1"
                     /translation="MAFLSFVIIQAFSVQAQKVFKVDYPSQADLKVFIVSYESQADLL
                     VYFVKYASQTNRDGLFFPVKYASQADFKLYFTDYESQADLKIFIVDYESKAGWRNKSK
                     QYLLKFKK"
     gene            48316..49365
                     /gene="mtnA"
                     /locus_tag="PLF20_00235"
     CDS             48316..49365
                     /gene="mtnA"
                     /locus_tag="PLF20_00235"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOX79470.1"
                     /translation="MKVNGKNYKTIWMEGKKVKLIDQNLLPFRFEIIELENWEGICKA
                     IKNMNIRGAGAIGVAAGYAMALAFVQSPGKGGEDYLWKSRKEIEQTRPTARDLFYATE
                     RVMQAGIAYGIEAAIEESEKLAIENEEEAKAIAIYGESLIPDSCNILTHCNAGWLGFV
                     DWGSALAPIYLAHRKGKKIFVYVDETRPRLQGSRLTAWELQNEGIAHVIVADNAGAWL
                     LSNNRVDLFITGADRIALNGDTANKIGTFEKAIVAKNFGVPFYVAAPLSTFDPACPDG
                     SCIPIEHRPLEEITEMEGLDEKHHFIKIRISSPGSEALNPAFDITPSSYIHSLITSKG
                     IIQPNNQAIRQLLFG"
     gene            49671..50516
                     /locus_tag="PLF20_00240"
     CDS             49671..50516
                     /locus_tag="PLF20_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOX79471.1"
                     /translation="MIYKKISDYNVPAVGLGTWEMGGGEQADYSHDRHWITIIRSAIE
                     NGLTHIDTAEMYGNGHTEELVGEAIKGFPRQNLFITTKVWLTHLRSNDLFSSFEKSLR
                     QLQTDYIDLYLVHHPNPDIPLEETLMAMNRLVEQKRVRAIGVSNFDISLIKEAVRISS
                     VPISAVQIEYSLLARNSGIYTQNQEKEIIPYCQENQIAVIAWRPLGKTNYIRLATNDV
                     IKKIAEKYHATPAQIALIWLIAQKNILAIPKTGSLEHLIENSKASDIDLSMEDGKLLR
                     EAFEE"
     gene            complement(50523..52844)
                     /locus_tag="PLF20_00245"
     CDS             complement(50523..52844)
                     /locus_tag="PLF20_00245"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX79472.1"
                     /translation="MRKFIVLSSCCMIWGVFIFSGLKSWAQEASIRGVIYEKESGEPA
                     IFVNVFLGKTTLGSATDVNGYYIINNIPAGSYNLMVTGIGYDSLVVPITIRKGEIITR
                     KLYISKKSIALEGVSISAEREAARTETRTSVVKVTPRQIKQIPSIGGQPDLAQYLQVL
                     PGVVFTGDQGGQLYIRGGAPIQNKLLLDGMVIYNPFHSIGLFSVFDTDILRNADIYTG
                     GFGAEFGGRISSVMDLTTRDGNKKRLSGKIDASTFGAKLLLEGPISKQKEDNSGSSSF
                     ILSVKNSYLEETSKTLYKYVNEDGLPFNFFDIYGKVSFNADNGSKVNLFGFNFTDRVN
                     NYKALSDFHWDAIGGGTNFVVIPGKTPVLLEGVISYSNYKVSLKETGKNDRTSQIGGF
                     NAGLNFTYFINKDELRYGFEFQGFNTDYYFFNSLNRVIQQKENTTEIGPYVKYKMHRG
                     KFLIEPGLRIQWYTSLSNISPEPRLAIKYNVNDRLRIKGAGGFYSQNLISATSDRDVV
                     NLFYGFLSGPDNLPEEFNGEKVTDKLQKAWHTIGGIELDLSNNVTMNIEAYYKKFTQL
                     TNINRNKIFDDTEIFADQPDELKNDFIIETGDAKGLDISIKFENDRFYFWGVYSLGYV
                     SRFDGNNTYSPHFDRRHTINLVAAVYFGSARDWELSSRWNFGSGFPFTQSQGYFEKIT
                     FENGIYSNPATENGNLAILYAELNTGRLPVYHRLDIDLKHKWELSQHSHLQLNIGATN
                     IYDRENIFYFDRIAFERVYQLPIMYNVGFTFSF"
     gene            complement(53055..53612)
                     /gene="idi"
                     /locus_tag="PLF20_00250"
     CDS             complement(53055..53612)
                     /gene="idi"
                     /locus_tag="PLF20_00250"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF002995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HOX79473.1"
                     /translation="MSDKVILVDINDKIIGEIAKIEAHEKALLHRAVSVIIFNSKGQM
                     LLQKRALNKYHSAGLWSNTACTHPFPGEDNKTAAQRRLKEEMGIHAEVFKIFDFIYHE
                     KLDNGLTEHEFDHVYIGISDEPPQPDPNEVSDFDYFERNFLKNELSSSPKHYTVWFAI
                     LFDRVYDFIDQQHLRERLITYNQTS"
     gene            complement(53623..54465)
                     /locus_tag="PLF20_00255"
     CDS             complement(53623..54465)
                     /locus_tag="PLF20_00255"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HOX79474.1"
                     /translation="MNKEFYLQTCYKISELITKRYSTSFSLAVSFLEKEQRSAIYAIY
                     GFVRLADEIVDSFHPHDKIFLLNKLTEDLYYSLGHNISTNTVLMAFADTVNKYKIPHS
                     HINAFLESMKRDLNITQYHKQEDLSQYIYGSADVVGLMCLRVFCHNEPSLYQKLELPA
                     QKLGSAFQKVNFLRDIKSDTQNLGRYYFPEMSTMSLNQQSKKIIEHSIEQDFKVAFPG
                     IRQLPGRSKLAVAIAYYYYNSLFIKIIHSSPEKILSQRIRISNIKKYLIMVKVCILYF
                     FKRI"
     gene            complement(54469..54867)
                     /locus_tag="PLF20_00260"
     CDS             complement(54469..54867)
                     /locus_tag="PLF20_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HOX79475.1"
                     /translation="MDSKGIIALWKEALLALIVLMGNTRGYRNHPQLLRLRQLRKPLK
                     SIDFYLNVVWQEAQNRGYNFDVSKFKQTETMTRIAITEGQVEFQLHHLLNKLKKRAVK
                     KYNEVKIIDMPVTHPLFYLVAGEVESWEKI"
     gene            complement(55311..56294)
                     /locus_tag="PLF20_00265"
     CDS             complement(55311..56294)
                     /locus_tag="PLF20_00265"
                     /inference="COORDINATES: protein motif:HMM:NF021537.2"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HOX79476.1"
                     /translation="MPKFLKIIVFLYCLLFGLQSFSQIKPAENSAFTVTTKDIDNFWV
                     AFDMLKNCNSSLDSIYCLKEYLFDNGTDGFKEFIRIYDYTSEDYLEAIRNYPKFFNSV
                     RANTLLTKEIYPEIIQLFQKIEKYYPNYIPLKICFVISPLRAGGTSTREFLFIGAEII
                     TSSNKVDLSEFGLNALGKILDSDTNIDKRLIYVIAHETIHHLQVNARLDNYDLLNRSL
                     FEGAADFIAWLLTGVRGNAYLYDYGISHEKELWMKFKKDIEAGVNTDNWMYNFDRVEN
                     DLPPDLGYFMGYRIVEAYFNKCLDKHQAVLDIIEMKNPEEFFKKSGYNPDS"
     gene            56879..58390
                     /gene="gltX"
                     /locus_tag="PLF20_00270"
     CDS             56879..58390
                     /gene="gltX"
                     /locus_tag="PLF20_00270"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOX79477.1"
                     /translation="MSGEAIRVRFAPSPTGPLHIGGVRTALYNFLFARQHNGKFILRI
                     EDTDRNRFVPGAEEYIIKSLSWCGIQFDEGVHLGGPFGPYRQSDRKHLYKQYIDILVE
                     NEHAYYAFDSHDDLEEAHEKNPNFQYDALSRMKMCNSLTLPKEEVKRRIAAGEPYVVR
                     ALIPIDIVIRVNDLIRGEVEVESKTLDDKVLFKSDGMPTYHLANIVDDHLMEITHVIR
                     GEEWLPSAPLHVLLYEFFGWEKPQFAHLPLLLRPDGNGKLSKRDGDRLGFPVYPLEWT
                     DPKTGEKSMGYRESGYYPEAFINMLALLGWNPGTEQEIFSLDELIDAFSLERVGKSGS
                     RFDPEKAKWFNQQYLRKRSDDELTQEFIPILKNLGVDCSWEYVKESIHLVRERVSFVK
                     EIWDQGWFFFKPPEKYDEEVIKKRWKPETSQILQKFSDTLLHLDTFDQVHLDETIQNF
                     LQENAVEMGALMSPLRICLTGGAIGPHLTEIMALIGKEETLHRIETALKKINH"
     gene            58394..58750
                     /gene="folB"
                     /locus_tag="PLF20_00275"
     CDS             58394..58750
                     /gene="folB"
                     /locus_tag="PLF20_00275"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOX79478.1"
                     /translation="MAWIALEQMEFWAYHGCFTEEQVIGTRFMIDLYIETDTSKAELS
                     DDLNDTINYQSVYKVVKNEMQIPSKLLEHLGRRILTSVFTRFPKISYAKIKISKLNPA
                     LGGKIGSVSVNLEEKR"
BASE COUNT        17531 a        10725 c        12064 g        18553 t
ORIGIN      
        1 tttgatgaaa agttttggaa aaatttttag ggtgacgcat tgtcgaagtc aggagttaca
       61 tgcaaaacga atttgcactc gaaggctgga tgtttatcaa ctacatcgca ttacattggt
      121 attatcggat ataccaacaa ttgctggcaa aacatgagct caatgggaaa ttttcaccta
      181 aagattttat tagcttctta accgaaataa aacgagtaaa aataaacgat aaatggtata
      241 ccgcagaaat taccaagaaa actaatgacc ttttgaagaa attagaacta cctattacgt
      301 aaattttgcg aagttagggt ttaatcgtaa attttcttat cgagattaat tatctacttt
      361 tgatgaatcg aattcttgat atatgaatga taaagaaatc cttattcagc tatattatca
      421 gaaaattaaa gcatccagta aggagctgac aaaaaaagaa gcctttaaag atttattgaa
      481 cagactttat tcttcggata aggaaattcg gagtataata gatagaatta ctcttggagc
      541 ggaaaccaca gttgttaata ttcccaaaaa ggataaattg caccgtggaa gtgccgacac
      601 cctttataat aatattatca tcgaattcga gaacgatttg aaggtttctt atcagcatgc
      661 taaggaacag ttggctggtt atttattggg tcaattcaga tcaggtaaag gctataattt
      721 cacactcata gtttcggatt ttatcaattg gaaagttctt gcacctgata tcagttgttt
      781 agataagctg gacgatttgc atgaaaatga attaatcttg aatgaaataa agagtgcttc
      841 ttttgtgctc gatgagtcga atgccgaaga attttactat tggatagaca ggtttttatt
      901 caaagaagag aaacaaaaag caacgcttaa aagcatcgaa gaatcttttg gatatcaaag
      961 ttccgttttt attgaatcat ttagagaatt gaacaactgg tttaatgaag cgaaaaaata
     1021 cggaatcgtc caggtttctt ttgaacaatg gtataaattt ttaagcattg cgtatggttc
     1081 attcgacggg agtgagagta tttttctgat tcatacctat ttgagcattt ttgcaaaaat
     1141 gttggcttat gccgttgttt ccaacgatga ttatatcagt gatgatgaga tgcgagaaat
     1201 cttgaatgga agcatttttc aatcttataa tatttcaaat ttcgttgata acgatttctt
     1261 tcattgggtc aatggtgatc gcgattatct caaattaaag aatgtattcc gtttgattgc
     1321 acaggaaatc tcgactttta atttttcgaa cgttgatgag gatattctga agggggtata
     1381 tcaagaactc attgatattg atacgcgtca ttctttaggt gaatattata ctcctgattg
     1441 gctttgtgaa cgaatagtga atgaatacga atttaagaag accgatagga ttctggatcc
     1501 tgcatgtggg agtggttcat tccttcgggc atttatccat agaatcaggg agttgaatcc
     1561 agatgctact gccgaggaaa ttaacaatca gatttatggc attgatattc atccgctttc
     1621 cgttcaaatt gctaaaacta ctttattgct ggctttgggt aagggtattt caagagcgag
     1681 aaggccaatt catttaaata ttatccttgc caatacttta cttgctcccg aaggagtaca
     1741 gaatgctttc ggcaatgatt ttgtgatgca gatcgataaa gataaatata ttatcaatac
     1801 ccagattttt gataatgttg aagtattcga tgcctttctt gatatttgtg aggaattgtc
     1861 ggagcagacc ttaaataaaa aggatatcga actatcgtta tttgaagata ttttgaagca
     1921 tcacaccaat ggtgggcttt gttcccaatt ggtcgagagt ttttataaaa tctatcaggg
     1981 agttaagagg gttaaagaaa aaggccgcaa tggcatctgg aaattcattc tttcaaatct
     2041 ttataaaccc ttcttccttg caaatagatt tgattatgtt ataggtaatc ctccttggtt
     2101 tacctatagt tctatcaaga ataaagaata tcaagactta cttgatgaac ttgcggtaaa
     2161 atataatgtt aaacctcaaa aagttgctaa ttatccgcat ttggagattg ctgctatttt
     2221 tcaatcatat tgtagcagtt attttttgaa tgattggggc aaacaggctt ttgtgcttcc
     2281 aagatccttt tttagtgccg atcatcacga aaattcgaga agtggcattg ccaaaggatt
     2341 ccgcttgagt agtatatggg atttagacaa tgtttctcct ttgtttagga ttccttgttg
     2401 tgtattcttc tctgaaaaga agcaaaaaga aaggataatg atgtcctcgg gcttagatgg
     2461 gaaaatattt gtggggaaat taccaacgca taattgtaat tatgcaatag caaagaatat
     2521 tttagttgaa actcctgtga aatggtatta cattaggcaa ggtcaatctt cggcattatc
     2581 gacgagaaaa atcgaaatgc aaaggaatgt gaatccatac aaaaatcttt tcaaacaagg
     2641 tgcaaccatt gtgccccggg cattttactt tgttgaactc gatcagcaaa tgcctgatga
     2701 tttcaacgat aggatcatca gtttaagaac ctcgaaattc gttaaggccg actccaaaga
     2761 cccttggaag ctcgagtttt caggtaggat ggagagccgt tttttgttcc gaacagcaat
     2821 ttcgaagtct atattgccat ttcatttgta taaacccgat tttgtggtcc ttccccttat
     2881 tattcaaatt aatgagaatc aatttaagaa aattgagata catactgccg atgatattat
     2941 gaatatgggg tatctgaatg catctcgatg gtttcgcaat gccgaaagaa tttggaatat
     3001 caataaaacg gaaaagtcga agaacatgtc aaccattggc cgacttaatt ttcataatgg
     3061 cttaactcaa caaaatttga atgcagaata tttagttttg tacaactcgt cggcaaaaga
     3121 tgccaatgca ttagtagtta atagatcaag tctcgacctt gagtttatat gcgaaagcaa
     3181 atcgtatgtt tattatttaa ataatgaaaa tgaagcgtat tatcttacgg ctttgttaaa
     3241 ttcgagtgtt cccaattttt taatgaagga ttttcagaca aaggggttat ttggcccgag
     3301 agatattcat aagaagattt taaatattta tttccccaaa tttgatgaga ataacaaatt
     3361 acatttgtcc ttagccgctt taagtaaatc gtgtcatgat aagactgaga aatatgtgga
     3421 aacaaacccg ccaaatagtg aattaacccc cattaagttg ggaaaatata gagtagatat
     3481 caaaagacat ttatcaaaag aaatggctga tatcgataag attgttagaa aaattataaa
     3541 ggagtgagga gtgcatttaa taaagattcc aataaatatt aaatattgaa attcaaaata
     3601 ttttattttt aaagattgtt ctgagatatt tatataaatt tgcttccaat catgaaacga
     3661 ctgaattttt tggaatagag aatttttgga attaatcgga atttgagcat gaaaaaagga
     3721 aacaacacaa ttatattttt ttgaggcctt caaggggttt actcaccact attacagcct
     3781 ctttaactca aaatagtttt ctaaatcaca atgaagaaac tttattgata gaaccgagaa
     3841 taattctttg aataactttt taccgaggtt acaacagaac ttcgaggcaa taagaatcaa
     3901 ttgtatacta attttgttca ttcaaaggcg aatttacttt gctaagctct gtaaaaatac
     3961 tttgaagtga gaaccatcaa caaatcattt aagcatgaga gtagttattc aacgcgtaag
     4021 tagagccaat gtaaaaattg atgagaggct gatttcggaa attggctatg gccttatggt
     4081 gttatgtggg tttgaggaag gcgatgaggt agaagacttg agatggatag ctggtaagat
     4141 ttctcgtttg aggatttttg atgatgaagc agggatgatg aacttgtcgg ttgtagataa
     4201 agctggtgag atgatggtaa taagtcagtt tactttgcat gccagtacca aaaaagggaa
     4261 tcgcccctca tatattagag ccttagaacc tgtgagagcc aaagaattat accagctttt
     4321 cgtcgacgaa ttggtatgtt acagcggaat cggcgtaaaa acaggcattt ttggcgctta
     4381 catgatgatt gaactagtaa atgatgggcc tgttaccatc attattgaca gcaagatgag
     4441 ggagtaattc aaagtttcaa catgaatttc tcgatgtcga taatgtatct tgtatgggta
     4501 ccttggccta taacgccctg atggtcgaac actcgaacct cgaagaacaa cttttttccg
     4561 tcgattgtgt ttagacgggc ttcgcaatga atttttacac caactctgat ggctcttaaa
     4621 tgctcgacat gcacttttgt tcccacggat gaatatccag gagctaaata atgagcaatc
     4681 ccttcgtaac aggttttttc cataaaagct atcatggcag gtgttgcaaa tacctcgagc
     4741 aaacccgaac cataatgtga agcagtgtct tctgccagta tgattttttt ctgttcgaaa
     4801 ttgagaggta gagcgggaac atgaatgatg tccatagatt tcaaatcaaa ttagaaatga
     4861 aaatgtacgg ttttcttgag ttgttcgttt aggctctgat acacagggca ggtaaaagcc
     4921 gaattgcgta tgatttgttt ttctttttcg gtgaaagggt gaggtggaaa atagagatga
     4981 ataataattt caccgactct tcgtggattc gattccataa tcttagtaat ctcggctttg
     5041 gttccatcaa gattaaatcc atgcgtgcgg gcggcaattc ccataatggt cagcatgcag
     5101 cttgccaggg ctgcggctaa taaatcagta ggagagaatg cctcccctct gccttgattg
     5161 tcgagtgggg catccgttat aatcgaactg cccgactgga ggtgtgtggc ttttgtacgc
     5221 aactcaccta aataaatggt ttcaatggta gccataagca tttcattatt ttattgagtc
     5281 gcaagataaa taataaaccc ggatatttca ggtttatcca tcaaacaagc ggaaaattat
     5341 ttcttttcag gagtagctgg ttttgtttca gcctgcttaa ccttatgaat ttcgttgcat
     5401 gaagcttttt tctctttttt gtcgcagcag gtattttttc cctgtatagg atgatcgcaa
     5461 ggttttatag gcttgggtgt gacagtttct gctttggcat ccttggtttt ggctttcttg
     5521 ggtggatcat catcattaag tacaatcatc gaatgatttc tctgaatcaa cgaaactgat
     5581 gcacttaggc tcgacataat aaaggcgcct aggccaaaaa gcaaaacgag ttttttcatt
     5641 tttcttaggt tttgaattaa gaacacaaag ttaatatttc cgtataaact cgggttagga
     5701 cttgtaaagt ttgaaaggca aaatacaaat tggttaaagt ttcgtataag attaaaaatt
     5761 gacaatttta ttttgcattg tgcttcactt tattcctttt ttttaatgta ttcgaagcca
     5821 gttgtttatt tctattttta cattcatgat gaaggctttc attttactgc tcctcataat
     5881 tgagacttcg agaagaaaaa gcattttttc gtcgaataat agataatgat tttcctgatt
     5941 caagggaatt attctctttt cgtcgattcg agcatatcat tttatctact ggttactatt
     6001 tacctttgca aggaataaag gaatgttttt tcaacttgct tcatcgtaag attctaagtt
     6061 ttacctttat tttatgaagc tcatcctgtc ttcatgagtt tattgtaacg atatatgaat
     6121 atgaataaat cggggagaag catatttaaa agattgggtt ccatcattaa atggatcctg
     6181 atgatttttg ggagtatata tttcgttatg attgttttga gttttaccag tgttcccttt
     6241 tgggcttatt atcgtttagg ttcaaaattt cccttggcct gccaacccaa ttacattata
     6301 gtgatggggg ctggtggaat gcccgggccc gagagtcttt tgcgatgtca ttatggaacg
     6361 caaatcgcaa aaagataccc tgaggcaaaa atcatcatcg cactccccac catatctgaa
     6421 gatatttata agagccatgc tcacaaaatg cttgaagaaa tggttattca tggcatcgat
     6481 agcagccgtt tcctatttga aacagaagga accaatacca gagaacaagc aaagaacata
     6541 gcaaaactta agggtgtggg tgccaaagat tgctttctta taattacttc tcctgaacac
     6601 gtatttcgta gcgttaaagt ctttcgcaag gtgggactcg aaaattgtgg agggcttcct
     6661 tcattttcta aagaactacc tacagaactt ttgctttcag aagatgaaaa tttgcagaaa
     6721 cagctcccac cagatcgttc tccttctctt cgatataata tgtggaacta tttgaagtta
     6781 gaaatatctg ttttgagaga atatgtagct attgtttggt attggctgaa tgggtggata
     6841 taagattcaa gacaaagaat gttcagtgat aaagattttg gctgtatcgg ctatagaatt
     6901 tctaatgggt atgaatgaat agtttgggaa ggtctctttg aatttttgat tggaataata
     6961 ataagacacg aaggccgagc gagcggtttc tgcggtgata gccgaaggtt tgccagtaac
     7021 aagactcttg agtgtgaata aacgccaggc aatctcagct gtaaatttat tgatgcgcac
     7081 cataggacga ggcttgccta atgcatcagc catcatatcg aataaatctt taaagcataa
     7141 attttctgag ttgaggataa aacgttcgtt aattatttca cttttcatca ataaaatgag
     7201 tgtatcggcc acatctcgaa catctacata tcctgtaact ccttcagtat aatactttaa
     7261 gccatgatag atttcggtga ataattgcga actacctttt tgccagtcgc ctggtccaat
     7321 aatggtagat ggatttacta ttactgcatt catgccttcg gcaatacctc tccaaatttc
     7381 tcgctcagca aggaatttac tcatggcata agtagtattt ttgggaattt cattgaataa
     7441 gtcatcttcg gttattgttt tttgctctgg attctggcct aacacagcaa cactgcttac
     7501 gtaggcgagt tttattattc ttttagccaa acaaaggttc acaatattta aggttccttt
     7561 aacatttgtg gtgaggattt tagatttatc cgctttcttg aaagaaacca cagctgcaca
     7621 atgataaact ttatccacac cgtcgaaagc ttcttcaaga gatccaatgt cgagcacatc
     7681 accttcgacc cattcgattt gtctgagcaa ttcatccgat ttattcgtgt aaaattgaaa
     7741 tattcttttt accgactgaa tcctatcttt cgaacggatg agtgctttta ctctatatcc
     7801 ttcgttcacc aaacgaaata atacatgaga tcccaccagt ccgctactgc cagtgactaa
     7861 tatcataagg caaaattaat catttagttc gtcatgaggt ttgttgttgg gtttcgtcat
     7921 atctttgcaa aagtttttat aatttatctt gatgcagata agtcacgata ttctgtagga
     7981 ttgatatgtc atagcaataa ttagagaagc taaattaatg tttgtcaaaa attttgttga
     8041 ggagctacgt tggagggtaa tggttcatga tatgacgcct gggaccgaag aataccttat
     8101 gcagggccca tcgtatggct atattggatt tgaccccaca gccgattccc ttcatattgg
     8161 ccatctggtg cagataatga ccttggttca ttttcagcaa tgcggccata aacccattgc
     8221 tttggtgggt ggtgcgacag gtatggtggg tgatccttct ggcagaagtg aagaacgtaa
     8281 tcttttaagc agggaacaat tagaatataa tcttgttggt ataaaaaaac aacttgcccg
     8341 ctttattgat ttcgaaacca aagtgaatcc tgcaactatg gtcaataatt atgattggtt
     8401 taaaggatac gaattcctgg attttatacg tgatgtaggc aaacatatca cagtaaacta
     8461 tatgctggca aaagactctg tccagaaacg cctcgatgcg ggaatgtcgt ttactgagtt
     8521 ttcttatcaa ttggtgcagg cttatgattt ttactggtta ttcaataata tggattgtcg
     8581 tcttcagatg ggcggttctg accaatgggg aaatattgtt actgggactg aattaatccg
     8641 gcgtaaggat ggtgggacgg cttatgctct tacaacccca ttattgaaaa aatcggatgg
     8701 gagcaaattc ggcaaaacgg ctgcgggagc gctttggctc gacgccgaaa aaacttctcc
     8761 ttatgctttt tatcaatatt ggataaatac ttcagatgaa gatgccatag attatataaa
     8821 aatttttaca actttaagta aagaagaaat agatgccctt tcagaagaac attggaaaac
     8881 tcctcatcta cgtattttac agaaggctct cgccagggaa attaccattc gggtgcattc
     8941 agaaaatgac tatcagatgg cagtcgaagt atcggagatt ctttttggta aaggcactgt
     9001 agaatcattg atgcaactcg atgaaaagac cttgttgaat tgtttcgatg gggtgccaca
     9061 atttcacgtt tctaaaacag aactcgaaac aggcattcct gcaatcgatg ttcttgctgt
     9121 gaaaactcag attttcccaa gtaaggccga agctcgaaga atgatgaaag atggtgctgt
     9181 attgctcaac aaacaaaaac tttctgagga tactttcatt gataactcaa tgcttcttca
     9241 aaataaatat ttacttattc aaaaagggaa aaaaaattat tttctcgtga tagccgaata
     9301 ataccatttt ttctgagttt aatttggcct tcctatccta taattatagg tttttcatga
     9361 ttgagctcaa ggatagggat aaagaagata aatcaataaa gaggggtgaa tcttgtatct
     9421 taccacgttt tctaaacatt agccataaaa gttagccaac gagaaaatta taataaatct
     9481 tctataatgt acccggagcc ggaatcgaac cggcacggga aaatcccatt ggtttttgag
     9541 accaacgcgt ctacctgttc cgccatccgg gcatgtcaaa gaacttgcaa aattaaacat
     9601 cttaccataa ttttcaaccc gtgaagatat tttcagttta aaatcatcat cgatattttc
     9661 gacttcagga tgttcctgca aaaagattca gtttcttttc cctaagttcg atacctttaa
     9721 aagataagat tcactcgcaa tgatacgagt tcttaaacga tttgaatctt tttgaattat
     9781 agtatttttg cagagaacac cttcaaaaaa cataactatc tgaaaaacat ggccagaaac
     9841 aaagttaaga ttttttcagg gcgatcgagt aagtatcttg ctgaagaaat tgcaaaggtt
     9901 tatggggatg aattggggaa agtaaatttg acgacattca gcgatgggga atttcaagtt
     9961 tcgtttgagc aaacagtgag agggaatgat gtttatattg ttcaatcgac attttctccc
    10021 gtagataatt tattcgagct tctcatgatg attgatgcag ccaaaagagc ttcggccgaa
    10081 agaattgttg ccgtcgtgcc ctattatgga tttgcccgac aagatcgcaa agacaagcct
    10141 cgtgtagcta ttgcggccaa attggttgcc gatctgcttt ccacagctgg tgttggaaga
    10201 ataattacaa tggatctgca ttccgatcag attcagggat ttttcaatgt acccgtcgat
    10261 catttgtatg cttccactat tttcgtgcct ttcataaaaa aattgaaact tcccaattca
    10321 gtcatggctt ctccagatac aggaggagcg aaacgcgcag cagcttatgc caaattcctg
    10381 aataccgagc tggtcatttg tttcaagcaa agaataaaac ctaattctat tggaaaaatg
    10441 actatcatag gcgaggtaga gggaaaagat gtgattttgg tggatgatat catcgatacg
    10501 gcaggaaccg tctgtaaagc tgctgatctg atgatggaac atggagccaa tagtgtcaga
    10561 gtatttgcta ctcatgctgt tctttcggga aatgcactcg aaaatattca taaatcctcc
    10621 cttattgaag tctatgttac agatacttta cccgtcaagg aaaccgaaaa gattaaagtg
    10681 ctttccatcg ctcccttgct tgccgacgta attgaccggg ttcatcgtaa taaacccatt
    10741 tcttcacatt tcgatttcac taccatactt tgattgattt ctgtattgtt tgagaatcat
    10801 attttaatag ctaattttgc aaactttttc aaaccttatt ttatcattta agtttttgat
    10861 tatgaaaaca atttctatga gcggttctct cagagagaac gtagggaaga aagatgctaa
    10921 aaaattacgt aaaaatggac ttgtgccggc taatatttat ggaggtgcaa atcaattcct
    10981 tatttccatc gaagaaaaac atctgcaaaa agttgttttt acccccgaag tttatttaat
    11041 tgagctggat atcaatggta caaaaagaca gaccattatt caggaagtac agttccatcc
    11101 ggtaaccgat aggattttac atgtggattt cctcgaagta ttgcccggga aacctataac
    11161 tttgaaaata cctattaaga ttactggtaa ttcgattggt gttctgaaag gaggaaaact
    11221 ccaaaaaaaa ttccgattgt taaaggtaaa aggccttgct tctgacatcc ccgaccatat
    11281 tactctggat gtttcagatc tcgatatcaa tcattccatt aaggtgaatg accttaaata
    11341 tgaaaagctc actttcctcg atcctgccag tgctattgtt gtagccgtta taccaacacg
    11401 agccgtgaaa gaaggtacac ctcaagcata acttttattg ttgttcttgt accaaaactg
    11461 tatcgtcttt tcaaaaagaa ggtacagttt tgtttttttg tttcattctt ttaatgaatt
    11521 attaaaattt acaggaaatg aaatatttga tagttggctt gggaaatcct ggatcggaat
    11581 atgcaaatga caggcataat gttggatttt tagtcatgga tgctcttgca attaaaacaa
    11641 attctcgttt ttctaccgaa aggtatgccg atgtggccaa ggttaaatat agaggccata
    11701 tacttgttct catcaaaccc aatacatata tgaatctgag cgggaacgcc gtgagatatt
    11761 ggctcgaaaa ggaaaaaatc tctctgcaac atcttttgat tgtgctcgac gacctcgatc
    11821 ttccacaagg ccaaatacgt ttgagagcta agggcagtgg cggttcgcat aatggattga
    11881 atcatattat tcaggtttta gataccgaag atttcccacg cctgcgtatt ggtattggtt
    11941 ctgactttcc caaaggtatg caggtagatt atgtgctggg tcagtggagt aaagaagaag
    12001 aaaaaatcat gaaacccgcc attcttgatg ctgccgatgc aattcttacg ttcgtgcatt
    12061 taggcatcga aagagctatg aatatcgtga atacgaaaaa aaattccctc accgatgata
    12121 attaaaaatg aatgaatttt cgacgtaatc attttatctc atttcgcatc caccctaact
    12181 atcccaagat gaatcaatta gccttttcaa agaatgtatt cttcagattc agatcaaact
    12241 gtagctcatt cctgaaagct aactttttcc aaaactttgg agcattcctt cataaccaag
    12301 gatgtaatta caatatacca ataatcaata atttataatg aatgaaggag ctgaccggaa
    12361 tattggccac ccgttaagga aaatttttca aatttgtttt gaaatcgaag ttcactgtat
    12421 gtaaaatatt gtcaagtagt ttatcaataa attgattcaa attgattaag gaggggaagt
    12481 atgtctatga gtgaggtata taaaagttaa catataaaat actttgatgt ttgtaaacat
    12541 ttaaaaatca atcttttatt tcattaagtt tttttaacaa aaaaataaat ttacctttgt
    12601 aaagggaaaa tttaaaacca aattaatatg caaatatcac atatagagca cattggcatt
    12661 gccgttcgaa gcatcgaaga gagtttaccg tattatcaag aagttttagg tttaaaatgt
    12721 tatggaatag aagaggtgaa ggatcagaaa gttagaacag ctttttttca ggccggtcag
    12781 accaaactcg aacttcttga atcgactgac cctgatggtg ccattggaaa attcatcgaa
    12841 aaaaggggag aaggaatcca tcatattgct tttgcagtaa aagagataga atctgctctc
    12901 gaagaggctc ggtcgaaggg gattcggctc atagacgaat caccgcgatt aggagccgag
    12961 ggattgcaca ttgctttttt gcatcctaaa tctacgcatg gagtattgac tgaattgtgt
    13021 gagaacaaat aaaaataaaa ttatacattt atatggctat tgaggataaa ttattggaat
    13081 tgctggaaaa aagacagaaa gccagtttag gcggtggtca aaaaagaatc gatgctcagc
    13141 atgccaaggg gaaactaacc gcacgtgaaa gaatagactt attgctcgac gacggcagtt
    13201 ttgaagaatt cgacatgttc gtaacacatc gctgtaccga ttttgggatg caggatcagc
    13261 tttatttgtc ggatggagtt gttacgggtc atggaacaat agatggaaga gtggtgtatg
    13321 ttttttcgca ggattttacg gtttttggtg gtagtttatc ggagtcgtat gcaaaaaaga
    13381 tttgcaagat catggatcag gccatgaagg taggggcgcc tattatcggg ctcaacgaca
    13441 gtggtggagc ccgtattcag gaaggggtat tgagcctggc aggttatgct gaaattttcg
    13501 aacgaaatat tcttgcttcg ggtctcatac ctcaaatttc ggctattttt ggtccttgtg
    13561 ctggaggagc cgtctattcg ccagctctta ccgatttcat catcatgagt caaaaaacca
    13621 gttacatgtt cgtaacaggg ccaaaagtcg taaagactgt cactggtgag gtagttactg
    13681 aggaggaatt gggaggagcc atgattcatg gtgcaaaatc gggggttgca cattttgttg
    13741 ctgaagatga gcgtgaaggc atactattga taagaaaact gttgagttac ctaccgcaga
    13801 acaatttaga agaccctccc gtgattcctt gcaacgatcc aattgatcgt ttggacgaca
    13861 gcctcaacta cttcattccc gataatccca ataagcctta tgatgtaaaa accattatct
    13921 atagtattat tgatgagaat gagtttttag aagtgcagcg gcattatgca cccaacatcg
    13981 taaccggatt ttctcgtttc gatggaatga ctgttggcat tgtggctaat caacccaatt
    14041 atctggccgg agtgctcgat atcaatgcct cgaggaaggc agcccgattc gtcagattct
    14101 gtgatgcttt caatatccca ttacttactc ttgtggatgt tcccggtttt ttacctggaa
    14161 cggctcaaga atatggaggg attatcattc atggtgccaa attgctctat gcttttggag
    14221 aagccactgt acccaaagtt accattattt tgcgcaaggc ttatggaggt gcttatgatg
    14281 tgatgagttc gaaacacctg cgtggcgaca tcaattatgc ctggcctaca gccgagattg
    14341 ctgttatggg tcctaaaggc gccatagaaa ttctttataa taaaaaaata caagaaattg
    14401 ccgatcctgc cgaaagagag gactttattc tgaatgccga aaaagaatat aaggataaat
    14461 ttgcaaatcc atataatgca gctcgatatg gatatataga cgatatcatt gaaccccgca
    14521 atactcgttt cagagtgatt agagcttttc aagcactggc tacaaaaaag gatactaatc
    14581 ctccgaaaaa gcattcaaat attcctctct aaataaataa tatttataag aaaccatgaa
    14641 acattttcgg ttttttatta ttattgccag cattttaccc ttgattggct tttcccaggg
    14701 tgtaaatagt ttaaagatca atgaaatact ggtaatcaac gattctaact atatcgacga
    14761 ctttggggaa agaaacggct ggatcgaaat ctttaatgct aactataatt atgtggatat
    14821 gggtggtatg tatctgacga atgatttatc caaccctacc aaatattata ttcctaaagg
    14881 tgatccccgt acagtaatcc ctccccgtgg attcatcgtc ttttatgcca ataataagcc
    14941 tactcgtgga ataatgcatt tgaattttga attgaagggt tcaaaagtcg ttgctatttt
    15001 cagttccgat ggaaaaactc tcatcgacaa attagacatc cccgaggagc aacaccccga
    15061 tgtgacttat gggcgcttga gagatggtgg ccctgagctt ggttatctcg aaaagacaac
    15121 ccctaaggca tccaatgtaa ctgagcaagt ggcaacacct gctgaaattt ttggggaaat
    15181 ggatcctcat ggcattgcaa tcactatcat tgcaatgtcg gtagtatttg tttctcttgc
    15241 cctgctatat atcttgttca agaacacttc caagatttat tctctcgact taagttcgat
    15301 atttaaacga aaaaaagggc ctcaaaagga agaagaaaaa gcgaagattg gagaatctat
    15361 tccaggtgaa gttgctgcgg ctattgctat ggctctatat caatatcatg cccagattca
    15421 tgacgtagaa aagactgttc ttacgattcg aagagtctcc cgtacctatt caccatggag
    15481 ttcaaaaatt tatggactca gaaaatcacc tcgttaaata tttatttagt tctattattc
    15541 aaagtcagtt taaataagat gaaaagattc aagtttaaaa taaatggcaa cacttacgaa
    15601 accgagataa ttgatatcga ggataatatt gcaactatcg aaatcaatgg ttctcgctat
    15661 acagtcgaag tcgatcaaac ccttactccc gtgaagacac cgaaactcat gcgtacacct
    15721 gcagttccct ctacggatat tcatcccgct gtttctaaga cgagtgcacc tataggccct
    15781 aaagggacgg gttatattaa atcgcctttg cctggtgtaa ttctcgatat tttggtcaaa
    15841 gagggtgatt atgtcaaaat tggccaaaag gtaatcgttc tcgaggccat gaagatggaa
    15901 aacaatatcg aaagcgataa ggaagggcgt gtggttagca ttaaaaagca aaagggagat
    15961 gcagtattgg aaggagatat attaatcgta attggagaat aaaatggctg aaaacgaagg
    16021 attcctgaaa tttatagtgg atcacttaga gcagtttttt agttacactg cttttgctaa
    16081 tatcactgtt ggccatatca tcatgatatg tgtgggattg tttttcattt ttttggctat
    16141 taccaaggaa tatgaaccct tgttgctggt tcccattgga tttggaattt taattggcaa
    16201 tatacctttt tatccaggat taaaagttgg aatttatgaa gatggaagcg ttttgaatta
    16261 tttatatttt ggtgtggttc aagggatata tcctcctctg atctttttag gcataggagc
    16321 aatgaccgat ttttcggctc ttctttcgaa cccgaagctt attctcatag gagctgcagc
    16381 gcagtttggt atatttggtg cttatattgc tgcgcttgct ttaggatttg ctcccaacga
    16441 agcaggtgct attggtatta ttggtggtgc cgacgggcca accgcaatat tcttaacttc
    16501 aaaattagct cctgaattgt tgggagctat cgccatttcg gcctattctt atatggctct
    16561 tgtacccgtc atacaaccac ctatcatgcg attgcttaca acccccaaag agcgtgtaat
    16621 ccgaatgaaa cctcccagag tggtatcgaa aaatgaaaaa attctattcc ctatcattgg
    16681 gctattgctt acctgcttta ttgtcccctc aggtcttcca ctcttgggaa tgctgttttt
    16741 tggcaattta cttaaggaaa gtggagttac caaacgcctg gccgataccg caaaaggccc
    16801 tttaatcgat atcgttacta tacttatagg actaactgta ggagcttcta ctcaggctac
    16861 tacttttctt accccaaagt gcattggtat ttttgctctt ggtgcttttt cttttatggt
    16921 agccactgca gccggagtat tatttgtgaa atttctcaat cttttcttga aaccagaaga
    16981 caaaatcaat cctttgatcg ggaattcagg agtatctgct gtgcccgata gtgccaggct
    17041 ttctcaagtt attggcctcg aatacgataa aaccaattat ttgcttatgc acgctatggg
    17101 accaaatgtt gccggagtaa ttggaagtgc tgttgctgcc ggtattttac ttagcttctt
    17161 gtattaatta tggaatgatt ggaaatgaca gagggtaatt tttagaaaat tataaagtgt
    17221 tgcgcgttta gaatttattt tgaaatgcaa ttataaagga ttctatgttg acgataggaa
    17281 ttgcaggtgg ttcaggttca ggaaaaacaa cagttgtcaa gaaaattacc caactacttc
    17341 ccccagactc ggtatctgta ttgtcgcaag atgcttatta ctacgataat agccatctgc
    17401 ctcccgatga aagggtaaag attaatttcg atcacccttc ttccatcgaa ttccctctgt
    17461 tgaatcggca tatcgattta ctcatggagg gtgaacgaat cccccatgcc tatatacagt
    17521 tatcttacct gtagcagggc aaaagatact atcactatat atcctcgtaa ggtcattatt
    17581 gtagaaggca ttcttattct tacagatcct gacctaagga gcaggctcgt tgtgaagatg
    17641 tttgtggacg cttcccccga tgaacggctg atgcgtatta ttcagcgaga tttggtagaa
    17701 cgtgggcgct cgctgcaaca aaccctcgag cattatcaga atttcgtaaa acccatgcat
    17761 gaacaattca tcgaacctac caaacgcttt gccgatatta tcgttcctca gggaggtgaa
    17821 aatcatgtag ccataagatt gcttgccgcc caaattaagg aaagactggg attgccataa
    17881 ttcgatgcat tcttttgcac gacttcatga tgttgaataa ttatatatga atatctgcgg
    17941 atcataaaca atgaaccccg ctgcaattcc ttatggatat tatttataaa cttgcgctat
    18001 ctaaaggtct tgcatcgagt tgaataaggt aagaatacgc gaaaagccct ataggtgagt
    18061 aaaatcaaaa acagagggta agtttatttt acagtgaagc tggtctctcc aaggagatca
    18121 tctccaagat aaacagccac ctgataggtc cccgagataa atttttcttg tagaggcagt
    18181 ttgttgtaat cagcgcacat tttcacagcc ttgttctggt aatcgatatt ggccattgta
    18241 gaatattgta attgttcacc ttggtaagta aatgaatatt catcgcctct gcttcgggta
    18301 agaatatttc cattgggatc ggcaattcgt acatagaaag tacgtgtgcc gggttgtgca
    18361 atgcggtttt cggcaatgat aaagcagact cttattttgt cgattcgttt tcctttgtct
    18421 gtaaattctt ccttacctcc gcttcgcaat cgtaatgttt cggcagtgag gctcgagatg
    18481 ctcaatgccg agccttgttt gatcttggtt gtaagcaatt ctttttcctg aaccaattgc
    18541 ttggcctttt gctgttcggc tatgatttct tctttcattt gtaggttttc ttcctgtagc
    18601 tgtcggttta cggtatacag gctatccatt tgcctgagat aggattgagc tactaacctg
    18661 agttggtcga gtttacggga aactttgatg tattcccatt tcgtatcgag gagtttcttt
    18721 atttcgagcg cacttgcctg aatgatgctg tctttgaccc tcaggctgtc ggcaagctcg
    18781 ccatatgcag cctttattct ttcgtgctcg gccattacag aatcgagctc atgtcggagg
    18841 atggcttttt cttcttcggc aatcttcttg atttgactta tctctgattg ggtactcacc
    18901 catttccatc caaaaaatat tgccaagagc aaaatgacta caccggcaat gattatgcct
    18961 ttgttcgatt tttgatcatt tgtgttttcc atttctctaa gtttttattg gttttggcaa
    19021 ctatattctc aattctttcg attttaatgc aaatataata gaaatattgg catactaata
    19081 aacaaccccg gtatttggcc ggggctgtga aaaatttgaa aatatcggct aaaaagtaag
    19141 aatcgcttat ttgatgattc ccatttcttt ccctacttta gtgaaggcgg cgattgcttt
    19201 atcgagatgt tccttttcat gagctgccga aatttgaacc cttactcttg cttgaccttt
    19261 aggaaccact gggtaggaga atcctgttac ataaattcct tcgtccaaaa gtctcgccgc
    19321 aaaatcttgt gccaatttcg catcgtaaag catcacggcg atgatggcac tttgtgtagg
    19381 ctttatatcg aatccaattt ccgacatacg ctggctaaag taagctgtat ttttatgcag
    19441 tttatcctgt aaatcgtttg tttctttcat catatcgaac atgcggatgc ctgcagcaac
    19501 aaccatagga ggtaaagaat tgctgaacaa gtaaggtctg gaacgctgtc gcaacatact
    19561 tataatttct tttttccctg tggtgaaacc accgatggct ccaccaaagg cttttcctaa
    19621 agtgcctgtg atgatctcga ttttcccctc caatttaaaa agctcagtga ctcctcttcc
    19681 tgtttggcca actactcccg cagaatgcga ttcgtctatc ataaccatag catcatattt
    19741 attggcaagt tcataaatct tgtcgatagg agcaacgttc ccatccatac tgaatacgcc
    19801 atcggttacg atgaggcgaa aacgttgttt ctgagaatcc ttaagtcgtt cttccaattc
    19861 cttcatatcg gcattggcat atctgtatct gactgcctta caaagtctta ctccgtcaat
    19921 gatggaagca tgattcaaag catcactaat aattgcatct tcctcgctaa gcaatggctc
    19981 gaaaactcca ccatttgcat cgaaacaggc agcgtaaaga atggtatctt cggtaccaaa
    20041 atattcggca atcttagcct cgagtttctt atgcaagtcg gtagtaccac aaataaatct
    20101 taccgatgac atgccaaagc catgcgtatc catcgcagcc ttcgctgctt ctattaattt
    20161 gggattattg gaaagcccca agtaattatt agcacaaaaa ttcaacactt tttggccagt
    20221 gttcagcgtt atttctgccc cttgcggact cacaatgata cgctcattct ttgctaagcc
    20281 ggcctcttca attgctgata attctttact gagaaattcc tgaaattctt tatacatagc
    20341 tgtattaaat taaatgataa gatgattttt taaagttgca aaattataga ttatttgtaa
    20401 cagatggttt aatcctccgt tctttcggga aatttataac gggcgatttc atctcgaatc
    20461 atctttaaat ctgccttaga gatgacgaaa atcccgtact tttgccgagc tatcgttaat
    20521 agattaacat ttataataaa ttcaaaaatt gcgatatgaa aaaaatttta gtaattggct
    20581 ctacgggaca gatagggtca gaactcaccc ttgccttacg gaaagaattt ggtggggaaa
    20641 atgtaattgc aggcattcgt aaaactgcac cttctaagca aattttggaa acagggccct
    20701 gtgaagtcgt tgatgctact atccgtgagc aaatggaacg ggctgtcgag aagcacgata
    20761 ttgacacaat tgtccacatg gcagctatac tttcggccgt gggagaacaa aatcccatga
    20821 tggcctggaa tgtcaatatg aatgggctta tcaatgtatt ggaatgtgcc cgcgatcgta
    20881 aaatggatag agttttggtt ccaagcagta ttgctgtttt cggacccggt acccctctcg
    20941 ataatactcc ccaggaaacc attcttcgac caaccaccat gtatggcatt actaaagttg
    21001 ccggtgagtt attgggcgac tattatgtgt tgaaatacgg actcgatgtg cgtggattgc
    21061 ggtatcccgg aatcatcagc catgagacac ttccaggcgg tggaactact gattatgcag
    21121 ttgctattta ttatgaagct gttaagaata aaaaatatac ttgttttgtt cgtgaagata
    21181 cccgattacc gatgatgtat atgcccgatt gccttaaggc tactatatca ttattaaaag
    21241 ccgatttcaa taaacttagg catcattgcg acttcaacgt ttcggctatg agtttttcgg
    21301 ttaaggaatt ggccgatagc attaaaaagt atattccaga attcgaggtt tcttatgagc
    21361 ccgatttccg tcaaaaaatt gccgacagct ggcccaatag catagacgat tcgtatgctc
    21421 gtgaagaatg gggctggaaa cctgattatg acctcgatgc catgacacgc gacatgctga
    21481 gtgcattaca aagaaagcac cagcaaggat ttatttaaaa aattgattta caataagatt
    21541 gaagccctgc attgcagggc ttttttcgtc gaacgttttt cagatcgatg attttggata
    21601 aagcaatttt ttaccaatcg agaagagaat ttttaatata aattatttta aatcagcgat
    21661 ttatattatt tccgattaat ttggttggat tcctccttcg aatcgtcaag gtatctatag
    21721 tttgcaataa tatcattcat ttaactttac caaataaaat atttcttcta atatttgcag
    21781 ttcgatagct gattaattat gttatgtttc gatgtccata cacatcattt ccgcgctgat
    21841 aatcgctcca tatttaatct gatattgaaa gaagatggtt cgatgattcc agaaatgtat
    21901 ttttcgagtg gaatccatcc ctggtatatt actgacaagt ggaagaagat tcttgaagat
    21961 cattttcggg aaaattataa gcaaagagtg gctattgggg agacgggcat cgatatgctc
    22021 aagggaaccg atttaaaact tcaggaatta gtttttatgg ctcatataga atgggctctg
    22081 aattatagca tgcctttggt aattcattcc gttcgttctt cgcaacgaat catcgagata
    22141 cttcgtgcaa tgaatttcca atttcctgtt gttattcatg gatttagaca aagtaacgag
    22201 cttgccaaaa aatttctatc cgaggggtat tatctttcgc tgggtgttta tgtaaattat
    22261 gcaggacaga agttgacaga agcgataaaa tatatccctt tagatagaat attgatcgaa
    22321 agtgatgatt ttgatggaga ttgttgtgaa atttaccacc aaatagctca tataaaagga
    22381 ataagtgccg atgaattgga caatgtagtc ttaatgaatg tgaagaactg ttttcggata
    22441 atcgatggaa aattggcttg aacgtacaga acttctatta gggaaggaga aactcgaggc
    22501 tttgaagagg tcgcatgttt tggtggccgg actgggaggt gtaggagcct atttggccga
    22561 aagcttatgt cgagctggtt tgggagaatt gactcttgcc gattctgata ttgttaaacc
    22621 ttccaatcgt aatcggcaac taatcgccct atcaagtacg gaagggaggt taaaggctga
    22681 agccatggct gagcgattgc gtgatatcaa ttctgagata catctccata taataactca
    22741 atatctaaag gatgatgtat tgaaagatgt tgtgtgtcga ccttatgatt acgttgcaga
    22801 tgctttggac acactttccc caaaactttt cctgctgata cattcggtaa attatggcca
    22861 taaaataatt agctcgatgg gagccggcag taaaattcac cccgaaagaa ttatggttgc
    22921 cgatatttcg gagactaata catgtccatt agccttatta ttaaggaaaa ggctcaaaaa
    22981 gcatgggatt tcgaaaggaa taaaagccgt tttctcttat gaagctgtaa tggaaggaag
    23041 tagaaaatat gtgaatgatg agctcaataa aaaaactatt gtaggtacta tatcatacat
    23101 gccggctatt tttggcttgt tcatggcctc tgtgatcatc cgcgatttaa ctggaacgtt
    23161 gcctgttcct tattgaatta tgagaatgta taatctttcg aatagatgga tcatttatga
    23221 agatgattta ggcggcaaga attcttgccc aagactataa aagtagttgc tctatgatga
    23281 tttatttata attttgcact gtcaaatgaa agataatcct gtcctgtagt gtaattggca
    23341 acacgtctga ctctggatca gaagactcca ggttcgatcc ctggcaggac aacaacaata
    23401 gagaaaagga ctggctcaaa aaaccaagtc ctttttttgt ttgtaataat ctttcgcagc
    23461 agttttgttg gtgaaatctt tcatttcttc cttgatgcct attaaagcat tagtgcaaaa
    23521 actctaatct ttcacgaatg ggtaaggcag gttgagtttt cttcgtttaa taataaactt
    23581 tatgcaaggg tttataacca actctaagta attgcatttc catttctata acttgtatat
    23641 gtcatagaat tgtgcaatcg ctattttttg ggctgttcac ttgggttttc cccaaaaggt
    23701 ttgatgaaat attatttgag agcagactag agaaattaaa aaagagctga acacaatgag
    23761 tgtcagctct catttaaaaa attcaatcga gctaattacc ttacaattag cttttgagtg
    23821 tatgaagagg attcattgac taattgaaga agataaatgc ctctacttag atttttgatg
    23881 tcgaggatat aatcattccc cgaggcgaaa ttttcggagg gtttcgaaat gatggtctta
    23941 ccggttaaat ccttgatgta gatattataa acttcgggtt tttcaacaca gatatgcaga
    24001 gaactggaag cagggttagg gaaaatatcg aggtgatgat ttttattgtt agaaatacct
    24061 tcgacgccgt agaaataaat ggtatggctt ttagtgattt cacagcctga actatttacg
    24121 gtaactgtga aagtgtgctc acctaagcca tattgatcga aagaaacggt aatagaagac
    24181 gtggtttcac cccctggttg ccagagataa gtagcctgtg ggtcgtcgat tgtcgcatca
    24241 agcacgatag gattgaaagg ccagaaactt gtatcgctgg gtgaggcagt aatattgacc
    24301 agaggtccta cctgtacata agcttgacgt tctaatgaat caatttgtcc gccacggtat
    24361 acgatgagtt taacattgtg agaaccacat tcggtatagg taaccgttgg atttttcagt
    24421 gaggaagtat cgggagtagc tcctgggaag aaccattgat aagtttcagg agtacctata
    24481 tatccacccg ttgtattatt tgtaaaaata acgggttgat taatgggaat aacggtgtca
    24541 ttggcagtaa aatcgacacg aaggtcgatg gcggcttgtt tagttccttg gaggaattct
    24601 ttgttttggg cctgaatacc ggcaacgaat tctacatctt caattgggca agtaggatcg
    24661 agggaaaaat taagagtaac tataaccaca tcaccacctg agaaatcaat agcagtgcca
    24721 ttttgatcgg gcaccatcag gcggtttacg aaatttacgt gattttgacc ttgccagtta
    24781 tatgaaatat gtgattgagt tacgaagaaa tgaagtttta atccagttgc tgtattgggg
    24841 gccactttgg tcacggtaat tactgcggtg taatcgagtc ctgaattggt aatttgcata
    24901 tccatggtaa aatcgcaggg tatggcaatt ctttgattgt aacggggaag gtaagtgggg
    24961 tacatgcttt ggctgtgatt gccaccaact actttactta tcccatcgaa aatagccgta
    25021 gggaatccgg taatcccata aaaggaattt cttgcattgc tgtattgatt agcaaaagga
    25081 tcgccattat ggttttcgac aactgctacc atacagccat ttgcgagaag atcgtcggct
    25141 cccatcgcgg cgcctggaca ataagtgcac catgtaccgg tgcctatttc aaggatcacc
    25201 atttcgcgcg gaacttgttg cccgaacacg aaagtcgcaa gcaacacaaa aactgaaagt
    25261 tgaaagattt gtttcatagg tttgtagttt taggttatta aggtattcaa ttaaagtaga
    25321 gggctaaatt aataaaaacc cgcattgtat tcaaaaaatt tattgtgaat aaaatgcggg
    25381 ttaagttttt aatgaatatt caatctttta tttgttgata acaaattttt gagtgaaggt
    25441 tcgaccgtta gtttcgataa ccagaatgta aattccatcg ggaagttgat cgaggttgat
    25501 cttttgcaga gtttgtgtgt ttattttgag gttatcgaga gcaaaaactt gctgtgcgaa
    25561 aagattgagt attctcatgt tcacggaggt gggttgttta gcatgaaatt ctatagtgaa
    25621 gtttccctgg ttgggattgg ggtaaatgct tatacctaag gatgacgcca gctcttctat
    25681 acccgtgcaa tccttgaagg taatgttgat atttgccgaa gctatgcaac catcggaatt
    25741 attcactgta accgaaatat tcttaacacc tccgatgcca acgccagtgc tatccacaat
    25801 tattgctggt gtagtttcac ctgtggacca caaataagtc gagttttcat tcgtagcatc
    25861 cagcgtaatg acatggttgt ggcaaatagt tgtatctcca ccaaataaga ctattggcga
    25921 tgcctttacg gatatggact gggaagcaaa gtttgaacaa cccgtggtat tatcggtata
    25981 tgaatatagg atttcggttt cgcctgctcc tatattcgaa ggatagaaga atccattttc
    26041 tacgccattt cccgaataaa tgccaccttc gggaactcca ccggttaatt cgatgggatc
    26101 ggtgttatcg cataagggag taaaaggagc aagtgaaact tcgggtaaat ccataacagt
    26161 aatgggttgg gatatagtag tagaacaacc aaattcattg gtataagtgt atgaaagtgg
    26221 atgttcgccg gcccctgccg tagaaggatt aaaataattc gtatctactc caatgccttg
    26281 ccataatccg ccttccgggt ttccaccagt aagttcgaat ggatctgtat tgacacaaac
    26341 agtctcgaaa ggtgtgagcg ttacaatggg ataatcgcta atggttaagg ttaccatggt
    26401 agctggtcct tcacaagtac ctgccgattg agtcacgtag tagttgaaag tcccaatttg
    26461 agtaatgcca gttgggaagg tgttgcccga ataaacctga ttggtaagta aactatcaga
    26521 ataccagcgt atgttttcgc cttcggcaat taaatcaggt actggcatgg catagcaggc
    26581 agaagtgtca tgagcaatgg gtgctgtagg ttctgcccat gcagtgatga aattttccag
    26641 ggtagttgta tgactttcgg ttccctttgt aaccgtgaga gtaacaggat atgagccttg
    26701 agtagcataa acaatgcctg tagggttctg cagtgtcgaa gtagagggtg tacctccttc
    26761 gaacgtccat aaccaggagg tgggatgacc tgatgaaaga tcggtaaaat caaccgaaag
    26821 gccttcgcag ccagaagtta tgtttgcagt aaaattggca agcagttgat cgatagctac
    26881 agtcatttcg tggcgatacc ggaaataatt ctgtttggtt gcgactaata ctagtttttg
    26941 gccagcggtt aagggaggca aattgaatac gataggagta atatttgcgg tttgaacgcc
    27001 aacaatctga ccgtcgaggg tgagagcaac ttgtgcgcct ggtgtagcct ctacagtaaa
    27061 tgtggtattg gtttgataaa ttactgaatc ataagtaata ttgatatgtt ggggcatttg
    27121 ggtataaagc acactccatg attctccatg gtgatggaag aggtagtaag taacggcttt
    27181 gttattcgta ttataaggcc aactcgattg acgcaagaaa tatttgccag cagcattccc
    27241 aaaagaagga agtacaccac gaggttcagg tgtagatgca tagcttggca tgaaatcagt
    27301 ccaccagttg tcgtaaaccc cccagacata agtgtcgtta acgaagctat aagaggtttc
    27361 ggaaggggcc aagaggccta atgcaccggc gttcaatcca ttcttggtat gtctgtggaa
    27421 tttttcggca aaacactcac tgccccagtt gtattttccc gttaggcagt tgatggtcat
    27481 cacaaaagta agattggtgt tggatagggc gttgatattg ctgttggtgt aggaaggttc
    27541 accccaacca gtttccatgc catggtccct atgttgaata ataaaggccc cattgtttat
    27601 ggcgttattg atacctgtgg cagatccacc cgtccaacct cctaactcct gtggtgtagc
    27661 cgggatatag ccctgtccgt taggcccaaa ataattgagt acagtgttag tgttggtagc
    27721 agttgaccac gggtcgacat taggatttcc ctgatagatt gcattttgcc tgactgggtt
    27781 tttgccaagt ttatttttga aaaagcctcc gactacctcc gaacaaattt ggaaccaacg
    27841 ttctgtctgc catcccaatg cagtgatagg acggttataa aacgtagtgt cggttggcgg
    27901 atttcgttca tagttcaata cttttgtaac catgacttgt agttcggtag tattacgggc
    27961 agtgatcctc gaaaatataa catcaggcat catgtctcca tcaacatcgg cataaatgtt
    28021 atctgaaacg caatagttgt tgtaaatagg tgaaatgata ctattggtag cattagtgcc
    28081 ataatcacct agcaataaac aggcaattgg ttttatactc cagttgttgt aagcattgtt
    28141 gataaaagtt tcgatagcgg tggtagtatt tccaccaatt tgggtaagtg taaatacttt
    28201 agtaagaatg ccctgctcgg ttctgaattt acgaatacta tctgcccagg tttgaaattc
    28261 gggaccatcg gggcaaatga tgatatattc gcattcatca tcttcagtgg gagcatagta
    28321 gctagcataa cgggcggcat agtcgatatg agacaaagag ctataattga ggaggttgtc
    28381 ggataaaatg gcatcaaaat aggggttgcg ataagcattt tctccaaatt gagaatttcc
    28441 tccttcaaaa cgaatttcta cgtcgatgtt cttataaacg ataagttctt tagtaacggg
    28501 attgtattga aaaggagtga ttccgagcag aacaacatct acgcctctaa tttttgaaac
    28561 ttccgataat ttaatcggtt gagccggata gaatgcattt acggaataga tggctggatt
    28621 tttttcataa tgcaatggac tgtcatcaga ttccagagga atacggggtg caggtccaac
    28681 ttctacattt tgaaattttt cgatttctac cgattttata tcaagaaccg gtgtagcacc
    28741 ttgtggaatg gcaataaagc ggctttcgcc agctaaattg ggagcacctt cgtcgttggg
    28801 caaaaagacc ccgctcaaca atacgctttt catttgctcg ccattaagtg taaaatcctc
    28861 taaatagaac tcgttcacta catattgaac ttcgacagcg cttgatttgg attctttcag
    28921 gttaaatcct tgcttcgtcg atgaattctg taatgtaatg gtttgagtct gggccaaaac
    28981 tccaacgctt tgcatcaaca taaagaagat gagcgttgca taaactgtaa gggagtagaa
    29041 ctttgttttc atcatgatat aaataaactg attgttaata cattagttat aagttgtaaa
    29101 tcaaaatttt ctttgtgagg tgaaaagaat tcgaattaat gtgcagcaaa tataatccat
    29161 tttttacagt ttgagtatcc aatttgatat ttttctgatt tctattgtct ttagttagaa
    29221 taattcttcc cgtaaagtcg attaattgga gttgaaaact cgacgattct tctgaagtaa
    29281 tttcaatgaa ttggtttaca ggagaataga cgatattgaa attattggaa ttgtttcggg
    29341 cataaggaat agaagtaaga ttgcttgtcc atattttcat tgcaggatcg cctaagatat
    29401 tgcagtcata aaagcaccag cgaagtgcac cttcctccca ttgaccaggg gcattgaccc
    29461 atggagcagt tttaatacgt gaatattggt gggtagcacc tatccgatcg taaccctccg
    29521 tataaagggc attgacaaac tcacggtgaa gatggaggga ggggccttcg gtttggcctt
    29581 cgttaaacca gccatatctc gaattaaatg ccccggcaac tgcaaaattt tcgattttca
    29641 gcatttcttc tgcaatgcag tcggaataat cgaatgctcc acagatacat ccatgtgtat
    29701 aaaccaaagt ataattatgg tcgatcccat tcacctgaga aaaattctgg ttgtttatat
    29761 cgctcgaaga aagtttcatg gcataatccc agtttgcatg gccagcatga tggataaatg
    29821 attttccagt gttgatcttt tgcagtaaat catatttcga ccagtaacct aaatgacttt
    29881 catacaatga atctatttcc tgatattcag gtataccatt agtgatatag ccgttatctt
    29941 catgatatcc aatcagctgt ttcaggtaat cttctccaaa agtcaaagga ttattgtaaa
    30001 gcagttcgcc agccatcaga ggtttttcga gttcattggg gatagggttc gattgatagc
    30061 taatgatttt atgaagcata ttctgaagtt cggtggtagt actgaaagga aggcgggcaa
    30121 cggccacctc tggcaataaa tcatcttctc ctatttcacc ccaaaggtcg tctccatcgt
    30181 cattccaagt tccatccaag gctgaataat agaggtcaga aggaatattg ttgtcttcat
    30241 agattgaaga tgaaaaaacc ttacagtaaa atcctcttgc tggaaaaatc tctacatcac
    30301 ctcccaataa gacgtatgat attccatttt gctggtattg agcaatgatg aaatttcgga
    30361 ttttttcctg atcgtcttga cctgccgtat tggtcagaac gttttcgaga gtatcgattc
    30421 tcaccgctat tccgttttgt tcgtaaagag aagccaatgg gaagaaatat tcggtgaaag
    30481 attctttggt gagtattagc aggtcgtaag ggttgttcaa ctttttatcc gaagcgtatt
    30541 gatgtatttc agaagcattt tggatcaggt ttttcaaatc tttatcagaa aatgagacag
    30601 ggtaaaggtt ttctaaagct tttctggctt tatgggtttc gtgacaaaag atcttgactt
    30661 ttgcatgttg atagacaatc actttttgct cttttggaaa atattctact ggagtcaaag
    30721 tggaaagagc aatagacaag cctttaaaaa aatgagtgct gagttttcct tgtttgctgt
    30781 tgggataggg ttttgaagac gaatagattt tttcgttttg gagccatttt gaatttcccg
    30841 ccaaggaata aggctttgcg tgttgcatag gaaggatgga tgaagcaaga tgaatttcga
    30901 aaggctggga atattccacc tccattgaat cggcttcatg ggctggaggc agcaataact
    30961 tcaccgcctg atagggaagt ccgggtgcac cagcttctcc agctatttga caaccagcca
    31021 gaaacaaaga tgaatatcct gaaatatctt taataatggg attattccag gagtaagaat
    31081 gctcgattac ttgtgtttta gcaaccatcg agaacataag gcattcggcc aagatcaagt
    31141 aaaactttct catagacatt ggatataaat aattacttgt tttaataatt gcttaaaaat
    31201 cattaaagac acatgcaaag ttcataaaat ttttaaaccc tcagcattta attgggaaaa
    31261 agaatgagta aatttcgatc aaaaaagttt aggggctcga aaggcccctg gttattgacg
    31321 ttttataagt ttacccaagc gaatcaattc ttcgctgtcg tataatttga aaacgtataa
    31381 acctgttggc aagtcattta acttaatttt tatctgtggc gaaggaacaa cttctattag
    31441 ctttactatt ttgccatcca tcgagatgag ttcgattctt gaaatgttcc ggtcagattc
    31501 gaatataacg aaattttgaa cgggatttgg gaaatatcgg aatgagaaat cgggataatt
    31561 ccttgtgtgt aaagaggggt cgacccttat ccagttcaaa ttgaccggct gattgaaggt
    31621 atctaattcg acagttacaa gaggatagag cagattatgc atttggaatt ctatggaatt
    31681 gtaaacattg atgctgctat ctacctcgaa ccaggttccc ataatatatg ccaatatggt
    31741 agtattttcg gttttattgg tcagtgtctt taaaacctta atatcggcgt aaggtagttt
    31801 catgattccc catccaacga cttcatgttt tacgctggcc ctaatgttga ttgcaaggct
    31861 atcgacaaaa taaccagaaa tggtatcatt gtaaggaatt ttggtcaggc tttgcggatg
    31921 actttcaacg attgaaccca tcgtataagg ataggtgtaa atgagttcgg gtgtaatata
    31981 gatgatagaa agtaataatg ctgtatccat aggcattaca gctcctataa tgccaatttt
    32041 atcgggagtt cgcgaaagaa tgtaataatt attatttttt tgaaaaacca gatttccttc
    32101 aggatattgg cctgcgaaag gcaggttagc taaatctgag atttgaatcg gagtagaatc
    32161 ggctgcaatc aaacccgaca gatcccagat ttgattggcg ccattaggag aaatatcagt
    32221 attttcgaga gtagtcggca cgtattccca ggtagaatag ggaataggag ggaaatcgtg
    32281 gtaagtaatc gtaatctgtc ccattatgag ccttgtgctt aatataagga ctaatgacat
    32341 cagaagaatt ttagttttca tggctggttt tgaattttag gaaagatgat acaaatttaa
    32401 tcataaatga ggccatttca acatattgtt ggaaagaaat cgtcataaat gaatggagat
    32461 ttctcttgaa atagagaata attttcttct ttcgaaagaa attataaatt tctcttgaca
    32521 aaggcctcga attgttgtaa ttttgcaaac cattataatc ataatcaatg atttaaaatt
    32581 tttttgatga tgagaaaata atcgatagca accatttctt cagatgattg aatttcagga
    32641 aaattgctta agatttatca caaagtcatt tgataggagt tatcatcgaa gaagaaatct
    32701 tttatcgtaa gaattcaaac agattaatca attttggaat aaccaatgaa actttcgcag
    32761 tttaaatttt atttaccaca agagctcatt gcaaactacc ctgccgatga tcctgatgca
    32821 gcgcgaatga tggtattgaa tcgaaagaca cagaccatcg agcataagca attcaaagat
    32881 gtaatagatt atttcgatga gggtgatgtt tttgtgatca acaacaccaa ggtttttccc
    32941 gccagattgt atggcgagaa ggaaaaaacg ggagcaaaga ttgaagtgtt tttgttgaga
    33001 gaactcaatc gtgagactcg tctttgggat gttttagtcg atccggctcg aaaaatcagg
    33061 attggcaaca agctctattt tggtaaaaat gatgagctgg ttgccgaagt catcgataat
    33121 actacttcga ggggccgaac cataagattt ttatacgatg ggccttacga aaagttcaga
    33181 aagactctga tgcagttggg tgagacacct ttacccaaaa ttcatcagcg gcctgtggaa
    33241 actgaggatg caaaacgtta tcaaactatt tatgcaaagt atgaaggtgc agtggctgcg
    33301 cctactgctg gtatgcactt tacacgcaat ctgatgaagc ggatggaaat aaaagggata
    33361 cgttttgccg agattacact tcatactggt gtgggaaatt tcagagagat tgaagtggaa
    33421 gacctgacca agcataagat ggattcggaa gagatgttta tcgaagaaga agcagctaag
    33481 ataatcaatg aagccaaatt gtctcatcgt aaagtttgtg cggtagggac tacttcgctc
    33541 aaagccctcg aaaccgcagc tactatcaat ggaaaaatta aaccttaccg aggatggtcg
    33601 aacaaattca tttttccacc ttatgagttt tcggttcccg atgcgatgat ttcgaatttt
    33661 catcttcctc tttcgcccat gctgatggtt gtttgtgcct tcggtggtta cgactttgtt
    33721 atgaaagcct ataaagaggc catcgaaaag aagtataaat tttttactta cggcaatgct
    33781 atgttgatta tttaatctca ttttctgaga tttccttcca gagttaaaga tttatgatgg
    33841 aaaaatatgc gatcattgtt gcagggggta gtggctcgag aatgaagtct gttactccca
    33901 aacaatttgc attgctggca gggaaaccag tcttgttgta tagtatagaa gcttttttaa
    33961 aggcctttga ggatatccgt atcgtgattg tgattcaaca gggtatggaa gcattctggt
    34021 ttacgatgaa aaaggaatac caactttcag aaaggattct gatagttcat ggcggcactg
    34081 agagatttca ttccgttaag aatggcctcg aaaaagttcc cgatgatgtt ttagtgggta
    34141 ttcatgatgc ggcgcgacca cttattgacg cagaacttat cggaaaaact tttcaaatcg
    34201 ccgaaaaaaa gaaaaattgt atacctgcca tatttgctca tgattctgtg cggattttgg
    34261 atgatgtgga aatttcacgt ccagttcctc gtgaaagggt tcaattgatc caaacgcctc
    34321 aggtgtttta ttccactcca cttaaaaagg cttatcgaac ttcttttcat gagcaattta
    34381 ccgatgatgc aagcatctgg gaatttgcgg ggaataaagt agaattttgc gaagggagcc
    34441 cattcaatct caaaataacc attccttctg attttgcctt ggccgaagcc ttactgatgg
    34501 cccgccaaaa ggaagctggt tctttctgat ttgaatttgt ttcagagaaa agggcttgaa
    34561 gtttcgtgct ttgctaataa acgacattga tgatttttcc tggaacgacg atgatttttt
    34621 tgggcttatt cccttgaaga taataggcag tcttttcgtt atttatgatt gcttgttcga
    34681 tttcatcgtt gttcaaatcg aggggaagag aaatattgaa ccgaaccttc ccattgaatg
    34741 aaacaggata atcgaaagag gtttccatta gatattctcc tttgtaatcg gggaggtggg
    34801 cataggtaat gctttcattg tgcccactca tttcccagag ttcttcggca atatgtggag
    34861 cataaggaga aatgagaatg ataagtggct cgagtatttg gcgcttattg cattttaaat
    34921 cagttagttc attgatggca atcataaagg cactgaccac tgtattgaat gagtatcgtt
    34981 cgatatcttc cctcaccttc ttaatggttc ggtgcaggat tttcagttct ttttcatcgg
    35041 ctggcgagtc gctcaagtaa aaatgattat gattatcatg aaacagtttc cagaattttc
    35101 ttaaaaagcg gaatactcct tctattccca tggtattcca tggttttgac tgttcaaggg
    35161 gacctaagaa catctcataa attctcaagg tatcggcacc atatttctcg atcaagtcat
    35221 caggattctg gacattatga agagatttcg acattttttc gatttcataa ccacaaacat
    35281 atttgccgtc ttccagaata aagtcggcat cggtgagatc aggacgccat ttcctaaaag
    35341 cctccacatc cagaatatca ttatcgacca ggtgaatatc gacatgaatg ggttgtacat
    35401 cgtaaatgtc tttcaggcta agcgagacga aggtgttggt tcctttgata cgatagacga
    35461 tgctgctacg accttgaatc atgccttgat tgatgagttt ttggaagggt tcgtcttcta
    35521 cggctaaacc tatatcgtaa agaaatttac accagaagcg tgaatagatc aaatgcccag
    35581 tagcgtgttc agaaccgcct atatacaaat cgacatttct ccaataatta actgcttcat
    35641 gtgagaaata cgccttatca ttatgaggat ccatgtagcg gaaataataa ccactggagc
    35701 cggcgaagcc tggcatagtg ttggtttcga gtggataacc cttataattc caatttttag
    35761 ctctagccaa aggaggctca ccatttgtag tcggcaagaa ggcatcgata aggggtaact
    35821 ctaagggcaa ttcattttca ggcaatgtat aagggatgcc atctttataa taaacaggga
    35881 aaggttcgcc ccaataacgc tgacgactga aaatagcatc gcgcaggcga taactgatgg
    35941 tcctatgacc aatacccatt tcttctactt tagccacagc tgccttaata gcatcattca
    36001 cgtccatacc attcataaaa ccactgttga tcatgattcc ttcctttgca tcataagagg
    36061 aatcgcaaag attgatatct aagatttctt cgttgggttt ttttactact tggatgatag
    36121 gaagttgaaa atggcgagca aagatgaagt ctcggctatc atgagcagga acagccataa
    36181 tagcccccgt tccatatccg gctaatacat aatcagcaat ccaaacgggt atctttttat
    36241 tgttgaatgg attgatggca taagcacctg taaacacccc tgttactctt ttcacctctg
    36301 ccatacgttc acgctcagat cggtttttag cccatgtaat ataggaagat acgtcataaa
    36361 ggtgtgaagt cgtagtaatt ttttctgcga aaggatgctc ggggctcaga accataaatg
    36421 ttgcgccgaa tatcgtatca gggcgagtcg taaaaatttc gatagcatcc tcataatcag
    36481 ctaacttgaa gaatatggtg catccttcac tcctacctat ccagtttctt tggatatcct
    36541 tgatggaatc gctccagtcg attttttcga ggccttcgag caagcgttcg gcgtaagccg
    36601 ttattctaag cagccattgt ttcattttaa ctctttcgac aggatagcca cccctcacag
    36661 ataatccatc acttacttca tcgttagcta aaacagtgcc cagctcggaa caccagttga
    36721 caatggtttc cgattgataa gccagacgat attccatcaa gatttccgaa cgtgtttttt
    36781 cagtggccga attccagtat tgagccgaaa aagagggaac tttgccggaa actgcttcta
    36841 tgccctcatt tccattcaat gaaaaatatt ccattaattc gctgatagga caagctttgt
    36901 cgagtttctt gtcgtaccag gaattgaaaa gctgtataaa agtccattgt gtccatttat
    36961 aataagaagg atcgcaggtt cttatttctc tgtcccaatc gaaagaaaaa cctaatctat
    37021 ccaattgctt tctatagcgt tcgatatttt tacgggtagt aatttcagga tgagttcctg
    37081 tttgaatggc atactgttcg gcgggcaacc caaaggcatc ataacccata ggatgcaaga
    37141 cattgtagcc tttcagtcgc atgtatcggg cataaatatc tgaagcgata tatcccaaag
    37201 gatgtcctat gtgcaaccca gcacccgaag gataggggaa catgtccaga acgtaaaatt
    37261 taggtttgct actggcattt tcggttttga aagtcttgtt attactccag tatttttgcc
    37321 acttgagctc gatttctctg aaattatact ccattcacta tacaaaattt gaaattgcaa
    37381 aagtaggaca ttacaacgtt tcgctctatc ctgtcttggg ttttaatgct ggccaagagt
    37441 ctttatgaat aaatagatgc ctgatgctat cagcactccg cctactgaaa catttaaaat
    37501 tcttctccat tttccttttg atatccgact taaataatgc gctaaaaaac ctttattgcc
    37561 attaaaagac tctctgttag aaaaatggaa aggaaaacga caaaatggga agaatcattc
    37621 gcgatgctat ttgccagaga gatgccctgt gccaccatcc aatataacca attgaatggg
    37681 ttgagggcat ttacaaaaaa agtttgtaac atgggcatcg aactataaga atggccagct
    37741 gataaatgtt ttttctagat gtctattgat gaaaataagc caattataac cagcaagata
    37801 gaccctccca atccgaagta aaaaagacgt tgtcttgttc aatgaattga gccaccccaa
    37861 ttccagctaa aatgaaaatg agaacatcag aaagaatgac accagcggcc gataaaaatc
    37921 cctgcgttcg actctgagta attgtatgat cgagaatgag aaaaaaagtt ggtcctatgc
    37981 ttatgccggc tatcaatcca aaagaaatcg acgaaaatat gctcgataaa ttttccatac
    38041 ttttattcac aaacttactt caatcttatt cttcttgaaa atttaaaaag attttaatcc
    38101 tctttttaat tgtgtttgaa tagatttgca taataaattc atcatcatga aaaaaccgca
    38161 acacattata tggtgtatcg gtcttttact gttcttattg agcagcttaa aggcgcagat
    38221 tccctcccaa ggattttcgt ttgtgtttat gacggacatt catctcacga gagatgacta
    38281 tgccgtagaa gggtttagaa aggcattggc tgtcgcttct gatttaaaac ccgattttat
    38341 tattactggt ggagatttgg tgatggatgc ccttgattgt gattatcagc atgctatcga
    38401 tcaatatgat ctttatttag gaacgatatc caattcccct attccttttt acaatgcgat
    38461 gggaaatcat gaaatttggg gctggcaaaa caaacatctc cgtcaggaag aatgggagga
    38521 ttatgggaaa aatcttttca aaagaagaat tggtccacca aattattcgt tcgaccataa
    38581 gggttggcat ttcgttgtta ttaatggaat cgagtacaat gattcgacga gttatgtagg
    38641 ttgggcttca gatgaaactc tcgatttcat tcagaatgat ttgaaaaatg ttccggttgg
    38701 catgccaatt attctcataa cgcatattcc tttgattacg ctcgaagctc aatgggagaa
    38761 aggtgcactg gcctgtaatc atcctatgga agtcattgga aattcgaagg aggttttgga
    38821 atgtttccct gctcatagcc tgcgattagt tttacaaggc catttgcatt attatgaaaa
    38881 aatggaaatc gatggaatta tttttattac aggaggtgcg gtatgtggag cctggtggaa
    38941 aggaaattac cgtgttaccg acgaaggttt tgtgctcatc aggataaatg gggaatattg
    39001 ccagacgcag tacatcgatt atggttggga agctatgaga taaattttct ctaatttttt
    39061 tacaattggt aaaatgataa taaattcctg tgtttacggg tttttcccgt gtctgtatct
    39121 attcccttgc ttcataccct gatgtaattt agatgtattc aacatccctt catataaaga
    39181 atccagcaaa atacattgtt cgggacggca aattctttta agcttcaaaa gatgttgatt
    39241 cagggccata tttatttctc cttcgagtct gccgatttct aatgccagat aatttaattt
    39301 tatcgtatcg ggagtgtctg atttcaattc ctgaatcatc ttttttcgaa taataagcat
    39361 cgaatcgcgt aaggatgcaa tctgaacatt gtactggcgt cgaatttgat cgtattggtt
    39421 agtttggttt tcgtcgagtt gtaaatatcg gataaccatt cccctacctt gacccactgg
    39481 cctttcaatt ggagcggaac ccgtgattat acgttgggtt ctggcatata gagtgatgaa
    39541 tgccacaatt aaaaagatta ttacagatga aagcaccaaa atccaaatct tgtatttctt
    39601 ttccatttat cgaattatta gcctttaaaa aaactttaaa tcattctaag tcactctctt
    39661 caaaaatcat ctcgagccct aaaggctcat tcgctattcc gtcttccata accaaaacct
    39721 cttgaagtgt taattcgtcg gttggtaaag ttggcaagag aattcttcca gtataataac
    39781 ccatgaggac agcaaagatg atactgaaac catagagaaa gatatttctg ggcttggatt
    39841 ggaaaatcca ggatgtagaa gatcgattta tatcctgggt atttaggcgg gcttggaaac
    39901 gagtgaagaa ataaggatcg ggttcgaaat ttacttttga ttcaacagcc gattcgatct
    39961 cttcggccaa tgtcgaacag ttggtgcatt tcaacagatg ttgttttgac gatataattt
    40021 cttgctggct taacctcttc gatttatacc gtggatacca tttattgaag ttctggcaat
    40081 cgattttcat ggctttagga gtttataata cttggcaaga tattttcgta agttgttttt
    40141 tgctcgttgt gtaagcgaat cgatggcttt tggagttttt tccattattt cggctacttc
    40201 agagtaggac aaatcctcga tatgcgtcaa aataaatgca attctctggt tttttggaag
    40261 tttattcaag gcatggttta aaatttttct tgtttccttt ttttctacaa aatgattgaa
    40321 agttctggga tgaagaatgt tattttctga ttccagggct tgtgcataaa ttttatgcga
    40381 agtatgtgaa aaaggaatag cactattttt acgtttcgag tttaatccat gattcagggc
    40441 tatgcgatat atccatgtgg atagagaaga ctttttacga aaccctgaga taccgagatg
    40501 aatcttcata aaaacctctt gggtaaaatc ttccacttct tcttcttgca taaagagttg
    40561 gtatccaatt gacctaatca tgggttcaaa attttcgacc aggatacgaa atgcattttg
    40621 gttgttttct tgcaaagcct ctatcaagga ggattcatct attttctgtt tacaaataat
    40681 agtctatttt tttcgagatg atttatttgc aaacttaatg ataacatctt gagataaaca
    40741 tttttaatag gggagcaaat cgtggcttgg gaagggattc ctcagcagaa tcgtatcagc
    40801 gattgggaat ggtttgatta ttacctcgag agttgagttg ctttgtagcc cttatgtcct
    40861 ccacgtttac ctgagaatct gctgggatag ttgtcgcaca ctccatcgtt gtttgcgtca
    40921 acaaaatatc tgttcattct tattcctgtt ctgtttccat agttgtcgca tactccatct
    40981 ccatttttat caataaaggc tccccttccc tgtatccaag ctcttgatgg ccaattgtcg
    41041 caaattccat ccttgttttg gtctaagaat cgaggagaag ttaaaggagt agaaccaata
    41101 tttttatatt gagactgatt ctttgaagtt gtttgagcca taattgcaat cggaaacagg
    41161 ctcatcatcg ttacacataa aatgatcatt ttggatttca tcttatttaa aattttaatt
    41221 gggttattct atttgaccat gaatgatgaa aaaaccctcg ggtaaatgaa atttttattt
    41281 gggaatagat tttccaaaaa aaagccacag aattttctct gtggtctgat gtggagcgta
    41341 ggggagtcga acccctgacc tttagactgc cagtctaacg ctctagccaa ctgagctaac
    41401 gccccaatgt tttatggcat gcaaaagtaa gctatttttt ttatttcttt gcttaatttt
    41461 atgaaaaatc ctttaccata ttgtcgatga tattagaagg cttgcttttt aaaatttatc
    41521 aaaggtagat tatgtcgaaa ttatacctgg tgccaacacc tattggtaac ctcgaggaca
    41581 ttactctgag ggcattgcgt atacttaaag aagtcaattt tatattggcc gaagatacgc
    41641 gttcatcacg aatattatta caacattatc aaattcaaac cccattggtt tctcatcata
    41701 agttcaacga acatcaatca ttgagaaagg taatagaaag gctaagaaat ggggaaatgg
    41761 cagccttgat ttccgatgct ggaactccgg ctatttccga tccaggattt ttattggtga
    41821 gagaatgcat tagagaaaat atttcgattg aatgtcttcc gggtcccgta gcttttatac
    41881 ctgcgctggt tgagtcgggc ttaccttgcg ataggtttta tttcgaaggt ttcctgccag
    41941 taaaaaaagg aaggaagaca cgaatggcac gtatggcaca gagagatgaa actactattg
    42001 tttatgagtc tccttaccga atagttcgta ccttaaagga gctttctgag attttaggaa
    42061 tggaaaggaa ggcctctatt tcgcgcgaaa tcagcaagat attccatgaa accattagag
    42121 gcacattttt ggaactcata gaatgggcaa aatccaaacc tttaaaaggg gaatttgtta
    42181 ttgttatagg aggcacaaaa gatgaaaact gacttaatag ccaaaaactg ggttcatctt
    42241 caggatttgc tttttcgtga tacctgggat aaagatttga agcgatatcg ttcagcttat
    42301 gtttatcggg gtttatccga taagaattat cagcttgtat ccagccttat taggcttggt
    42361 gggccttacg ataggctcga acggcatctg ctgcgaaatt ttattaaata tgcccgtcgt
    42421 ggctcaacag gcgagtcttt gtggaattgg ctcgtattgg ctcaacatca tggtttgccc
    42481 acccgtttga tcgactggac ttattcgcct ctggtagcct tgcattttgc tacggccaat
    42541 cctttgaaat ataaagatga cggtgtgata tggtgtgtca attatgtgaa agccaatcat
    42601 tttttgccgc aaagcttgct cgatatcatt ctcgaggaag gatcgaatgt atttactcct
    42661 tacatgctcg ataaagtttg taattcattg agtaaactca gtgagttaca gtcggaaccc
    42721 ttcaatattt ttcttgaacc tccttcactc gatgagcgca tagtgaatca acatgctctt
    42781 tcttcgctca tgtcgactgc aaaagctcat ctaggtgttt ggttgcagaa tcacctcgat
    42841 atttacttcc gcatcattat tcctgccgaa ctcaaatggg aaatcaggga taaactcgat
    42901 cagtcgaata ttaccgaaag agttcttttc cctggcctcg acggactgag tctatggctt
    42961 aaacggaatt atagcccaaa agatattcct ttcgacattt agttattttt gaaaatgtca
    43021 tatacttatg aatatccacg accctgtgtt accgttgatg ttgcattatt tcgtaaacat
    43081 cacgacaagg aggagatttt actgatacaa cgtaaaaatc ctcctttcga aggcatgtgg
    43141 gctttaccag gaggatttat caacatggac gagaatcttt ctgaagccgc ctttagagaa
    43201 ttgaaagaag aaacgggttt gacccctcgc actcttttcc aatttcgcac ttatggagat
    43261 gtttatcgtg atccgcggca tagaaccatt acaatagttt attatggttt tattgatttt
    43321 gaagtaagcc agcctttgtc gggcgacgat gccgccgacg cagcttggtt ctccattcaa
    43381 aaactgcctc cacttgcttt tgaccacact caaatcatca atgaacttat agaatatctt
    43441 atatcaaatc atcaaatcgt gttatgaaaa agttaatttt tccctttgtg ttattcctga
    43501 cattttttaa acttcatgcg caagtcgtcc tgcatgattc attatggccc gaacctggca
    43561 cttttattta catgagaatt gtggatgatc ctgccagcat gcactttacg cattccgggg
    43621 agaatgtgaa ctgggacttt acaagtcttg tttcttccga tcaggatact attgcctact
    43681 tggatccttc cactacgcct tattttggaa gttttccaca atcgaacctt gctctaggca
    43741 tgagcccaac tgaaatggct tatttgcaga atgatgaatt tgatagtcaa ctgattggaa
    43801 taaatggtga tccgggcact ggtacgccat tgactgttcc tcttcatcct tctcttcact
    43861 tgtttcattt tccctatact tatggggata gcatattctc cactgctcga gccattattc
    43921 gtggtacggg cgctatgttt ggccagcctg ccctcgattc catgaaacta gtttctacta
    43981 ttattaccca tcgtaaagta atggcgtggg gattgttgct gctgccctgt agtggatttc
    44041 cagagacctt gctcgagaaa ggcataatca ctcgaattga cagtgcatgg accaaggtgc
    44101 catttttcgg atggattcct gtcccaggct tcccaatgat aaccgtagat acaatgtaca
    44161 actggtttac cgctaattct atacatcctt atgctcagtt ggtagttgac caatctggta
    44221 caccctacag cgcaagttat tattcgggca atttagaggc aatcgatgaa tattcacact
    44281 cgcagaattt cccgtttgta gtaagtccaa atccggttga aagcgaagtt tcattatttt
    44341 cgactcaaac aggttggagc ggtaaacttt tcgattatac cggaagattg cttaaggacg
    44401 atattaaagt gagaatagga acgaccagag tcgatgtttc agggctcaag acaggggttt
    44461 atctcttggt tttgaagaat aatagaggag aagtaaaaac cataaaaatt caaaagcgtt
    44521 gatttagcag tttgcaatcc attgttttac ttcatccaac gaaggcatgg ggatatcgat
    44581 cttatttttc ttgcgcaagc ctcctaactg gttgaaaaga gaacctggat ttgcctggct
    44641 gagggcttcc ttgcttgtgt accctgcctg aataaccagg ggaatccatt cacgaggtat
    44701 tcctgtatct tccagatcgc ttgggggagc aacttcctga cttttttccg gtcgcatttg
    44761 agggaataga atcacttcct gaatagacga agaattcgtc atgatcattg ccagacgatc
    44821 tattccaatc ccaagaccag ccgtaggtgg cataccatac tcgagggctc tcagaaaatc
    44881 ttcgtctaac accatggatt cttcatcgcc tctgcgtctc agttctaatt gtgcttcgaa
    44941 acgctgacgc tgatcgatgg gatcattcaa ttcggaaaaa gcattgcaaa tttcttttcc
    45001 gttacaaatc atctcgaatc tttccaccaa accagttttg gaacggtgtt tatgcgccag
    45061 aggcgacatt tcgataggat aatccatgat aaatgttggt tgaattaaat aaggttcaca
    45121 tttttcgccg aagatctcat ctatgatttt tccttttccc attgcatcgg taatttcaat
    45181 attcaagcgg gttgcagtct ggcggagttg ggtttcatcc atgtcggaga tgtcgagacc
    45241 cgtaaattct tgaatgatac cagtcatggt aaatcgtttc caaggtcgtt tgaaattgat
    45301 aacatgatca cccacctgaa tttgtgtgga tgaatgaagg tctaaagcaa ttttttcgac
    45361 catttcttca accaggtcca tcatccaata atagtcttta taggctacat aaagctccat
    45421 ctgggtaaat tcggggttat ggaacctgtc cattccttcg ttgcgaaagt ctttcgagaa
    45481 ttcataaaca ccatcgaatc caccgacgac caggcgcttc aaatacagtt cgttggctat
    45541 tcgaagataa agagtggcgt cgagagcatt atgataagtt ttgaaaggcc gggcagcagc
    45601 tcctccatat aaaggttgaa gaataggtgt ttctacctcg aggtatcctt tccaatttag
    45661 aaaatcccgc atcgaattga ccatttgcgt tctcttaata aatatttgcc ttacatgcgg
    45721 attgacgatc aagtcgagat atctttgacg atatctttgc tcgggattct gaaaagcatc
    45781 atatactttg tcgtcttttt cttttacaat agggagtggt cgtaacgatt ttgccagcag
    45841 ttcgagctct ttcacgtgta tggtaatttc tcccatctgg gtaatgaaaa caaagccttt
    45901 tacccagata atatcgccta tgtcgagcag ctttttaaaa acgatgttgt agaaggtttt
    45961 atcctcgttc aggcatagat catcacgttt cacgtacaac tgaattcttt ctgtagaatc
    46021 ctgaagttct aagaatgaag ctgcacccat tacgcgtcgc gacatgattc ttccagctat
    46081 gcaaacttcc tgataattat tgagttccgg attataattg gtcaggattt cgtttgccat
    46141 tgcatttacg ggaaatacac ctgtgggata aggatcgatt cctaaattca ttaattcctg
    46201 aagcgcttgt cgcctgatga tctcctgttc acttaatgcc attacttata aattttgggg
    46261 tgcaaagata aggatattca gcttcaatca tttaattgag gaagtttcgg ccttattgtt
    46321 tttacatcat gaatttaatt ttttccataa gaatgctcga tgaaagatat ttcttggtaa
    46381 ttttatgaaa aatttatttc catcgattcc atcctgttat atttatgtca aaaacatcga
    46441 agaactatac aaacaaaaat gttactccgg catcgggtaa aaaagcaagc aaagcgaaag
    46501 gggaaatgag cgaaacttcg tattggattt attttttgat tattttgggg attgcgctat
    46561 ttctgcttta tttgtttaaa taagtgattg tggattttct atcagcagaa agaccttatc
    46621 acctcttctt acgggagcac ttaccttgaa ggaacatatc cggcctttcg gtgcaagagg
    46681 gattttatcg tactcgagcc taatttcctc aatcgtacct tctactactc ctgaagtagg
    46741 accattgata gaatatttat cgccaatctt caaatcgttt gtttcgagtt ttacttcagc
    46801 tacacctaat ttagagaaat aattgagcac tttgccaaga tattgtttct ttatagtagc
    46861 ctgcgaaccg tgttgttgag tccattcacc cagttctctg cccagatagt acccttccca
    46921 aaaaccacga ttataaacgg tgcgcaaacg ttcagtccat ttggctactt tttcctgtgt
    46981 aaaggtttta ttcaaaactg cctctactcc ttcatgatat acctgagtga ctgttttgac
    47041 atattcgggt gatctgccac gaccttcaat tttcaaaatt ttgactcctg cagccaaaat
    47101 ttgatctaaa attcctatgg tgcataaatc ttttggcgac atgatgtatt cattatcaac
    47161 ctcaagaact gttccggttt ccttgtcggt aacatcatat ctacgacggc atatttgcag
    47221 acatgctccc ttattggccg acgaattcat tgtatgtaag ctcagataac atttgcctga
    47281 tatggccata cataaggctc catgaacaaa aacttcaatg gcaacgaggt ctcccgatgg
    47341 accacgaata tcatacgttt ttattgcatc tacaatttgc tttatttgct ggagattcag
    47401 ttctctggcc agcacaacta catcggcata acgcgagtac cattttacgg cttctatatt
    47461 ggtaacatta cattgagtag aaagatgaac ctctattcct ttttcaacgg cataatttat
    47521 aacggcagga tcggaagcta taatggccga aacacctgat tccctggccg tatctattat
    47581 gttttgcatt tcttgcaatt cttcatcata aatgattgag tttaaggtta aatacgaacg
    47641 tatgcctttc tctttgcaaa ttcgggaaat atcattcaaa tcggataaag taaaattgac
    47701 agaagacctc gagcgcatat tatgcttgcc agccccaaaa tataccgcat tggcacctcc
    47761 ctgaatagca gccattaacg attcgaacga acctactggc gacatgatct cgatttgttc
    47821 tctcgcttcc aattgatttt tcttctttaa attgatgaat gggtaagaag ccgaaaatta
    47881 ttccttaatt ttgaaatgaa ctacaaaatt agtcattcaa cccaacttaa ataatttaca
    47941 taaatgaggt tcatattgaa accaataaaa cttatggcgt ttttatcttt tgttattatt
    48001 caggcatttt ctgtacaagc gcaaaaggtt tttaaggtag attacccttc tcaggctgac
    48061 ttgaaagtat tcatcgtttc gtacgaatcg caggctgatt tattggttta ttttgtgaaa
    48121 tatgcatcac aaaccaatag ggatggactg tttttccccg taaagtatgc atcacaagct
    48181 gatttcaagt tatattttac cgattatgaa tctcaagccg atttaaagat ttttattgta
    48241 gattatgaat caaaggcagg atggcgcaat aaatcgaaac aatacctgtt aaaatttaaa
    48301 aagtaagcaa ttggaatgaa agtaaacggt aagaattata aaaccatctg gatggaaggt
    48361 aaaaaggtga aattgatcga tcagaaccta ttgccttttc gattcgaaat cattgaatta
    48421 gagaattggg aagggatatg caaggccatt aaaaacatga acataagagg cgctggggca
    48481 ataggagttg cggctggtta tgctatggca ctcgcttttg ttcaatcccc cggtaaagga
    48541 ggagaagatt atttgtggaa atcccgcaag gagattgagc aaactcgacc tacagcgaga
    48601 gatttgtttt atgctaccga gagggtaatg caagcaggaa ttgcttatgg cattgaggca
    48661 gcaatagaag aatccgaaaa gttagctatt gaaaatgaag aagaagcaaa agcgattgcg
    48721 atttatggag aaagtttaat acccgattca tgtaatatat tgacgcattg taatgcgggc
    48781 tggttaggtt ttgtagattg ggggagtgca ttggcaccaa tttatttagc acatcgtaaa
    48841 gggaaaaaga ttttcgttta cgttgacgaa actcgtccgc gtttacaagg ttcaaggctt
    48901 acagcatggg aattgcaaaa cgaaggtata gcgcatgtta tcgttgcaga taatgcgggg
    48961 gcttggcttt tatcgaacaa tagagtggat ttgtttatca cgggggccga tagaattgcc
    49021 ctgaatggag atacggccaa taaaattggg acattcgaaa aggccattgt ggcgaaaaat
    49081 tttggggtac cattttatgt tgcagcacca ttgtctactt tcgatcctgc atgcccagat
    49141 ggttcctgca tccctatcga acaccgtcct cttgaagaaa taaccgagat ggaaggttta
    49201 gatgagaagc atcactttat aaagataaga atttcttctc ctggctctga agctttgaat
    49261 ccagctttcg atatcactcc atcttcctac atccacagct tgattacttc gaaaggaatc
    49321 atacagccta ataatcaggc tattaggcaa cttttatttg gttaaccaca aaatgcaatt
    49381 tttataataa ggaattactc caattggata ttaattttag aggataacga gaataatgta
    49441 aatataaatt aattttcctc agttttcgat agagaatttt tttcaggtta ttttataaat
    49501 ttatccaaaa tcccgaaagc ctatgaagac atgattaaaa caagtaatta tgtataacga
    49561 aattaggaat tttacgaaat acatgatgga ggattgaaga ttataccaga tgcgggtaaa
    49621 gttttatttt tagttgcttg attttataaa atctaaccgt ttttaaagca ttgatttaca
    49681 aaaagatttc agattataac gttccggcag tcggtttagg aacttgggag atgggaggtg
    49741 gcgaacaggc cgactattct cacgacaggc attggataac tattattcga agcgctattg
    49801 aaaatggcct tacacatatc gacacagctg aaatgtatgg caatggtcat acggaggagc
    49861 ttgtagggga agccataaaa gggtttccac gtcagaacct gtttattact accaaagtct
    49921 ggcttactca tcttcgttcc aatgatttat tctcctcatt cgagaaaagt cttcgtcagc
    49981 ttcagacaga ttatatcgat ctctatcttg ttcatcatcc caatcctgat attcctttgg
    50041 aggaaaccct gatggccatg aataggctgg ttgagcagaa gcgagtaaga gccatagggg
    50101 tgagtaattt cgatatctcc ttgataaaag aagcggtgag aatttcctca gttccaattt
    50161 cggcagtaca gatagaatat agccttttag cgagaaattc tgggatttat actcaaaatc
    50221 aggagaaaga aattattccc tattgtcagg aaaatcaaat tgcggtaatt gcatggcgtc
    50281 cattgggaaa aaccaattat atccgattgg caaccaatga cgtaataaaa aaaattgctg
    50341 aaaagtatca tgctactcct gctcaaattg cactcatttg gctcattgca caaaaaaata
    50401 tattagctat tccaaaaact ggttcattag aacatttaat agaaaattcg aaagcttccg
    50461 acatcgatct ctcgatggaa gatggaaagc tattacgtga ggcattcgag gaatagaata
    50521 gattagaaag aaaaggtgaa gccaacgtta tacatgattg gcaattgata gacacgttca
    50581 aaggcaatac ggtcgaaata gaagatattc tcgcggtcgt agatatttgt agctccaata
    50641 ttcagttgca aatgagaatg ttggctgagt tcccatttat gtttcaaatc aatgtcgagg
    50701 cgatgataaa cgggtaagcg gccagtgttc aattcggcat ataggatggc taaatttccg
    50761 ttttcggttg ccgggttgga atagattcca ttttcgaaag ttattttttc gaaataacct
    50821 tggctctgag tgaaggggaa tcccgagccg aagttccagc gagagctaag ttcccagtcg
    50881 cgtgctgacc cgaaatatac agcggcgacc agatttatgg tatgccgcct gtcgaaatgt
    50941 ggagaatagg tattgtttcc atcgaatcga cttacatatc ccaatgaata aactccccag
    51001 aaatagaatc tgtcgttttc gaattttatc gaaatatcca aacctttagc atctccggtt
    51061 tcgatgatga aatcattctt caattcatcg ggctgatcgg caaatatttc tgtatcgtca
    51121 aaaatcttat tgcggttgat attggtgagt tgggtaaatt ttttatagta tgcctctata
    51181 ttcatggtta cattattgga taggtcgagt tctatacctc ctatggtatg ccatgctttt
    51241 tgcaatttat cggtgacctt ttccccattg aattcttcgg ggaggttgtc ggggcctgaa
    51301 agaaatccat agaagagatt cactacatct cgatcacttg tagcactgat caggttctgg
    51361 gaatagaagc ctcctgcgcc tttaattctc aagcgatcat tcacattata tttgatggcc
    51421 aaacgtggtt caggcgatat gttgcttaaa gaagtatacc attgtattct caatccgggt
    51481 tcgatgagaa attttccgcg atgcatctta tattttacgt aagggccaat ctcggttgta
    51541 ttttcctttt gttgaatgac ccggttgagg gaattgaaga aataataatc ggtgttgaaa
    51601 ccttgaaatt cgaagccgta acgtaattca tccttgttga taaaataggt gaaattcaaa
    51661 cccgcattga aaccgcctat ctggcttgta cgatcgtttt taccggtttc tttgaggctt
    51721 accttataat tagaataaga aataactcct tcgaggagta cgggagtttt tccgggaatt
    51781 accacgaagt ttgtgcctcc accaatggcg tcccaatgaa aatcagataa ggctttataa
    51841 ttgtttaccc gatcggtgaa attaaaacca aaaaggttca ctttgcttcc gttgtcggca
    51901 ttgaaagata ctttgccata aatatcgaag aagttaaatg gaagtccatc ttcatttacg
    51961 tatttgtaaa gggttttaga agtttcttcg agataggaat ttttaaccga aagaataaag
    52021 gacgaacttc ccgaattgtc ttctttttgt ttgctgatag gtccttcgag cagcagtttt
    52081 gctccaaagg tagaggcatc gatttttcca ctcaagcgct ttttattgcc atcgcgggtg
    52141 gtgaggtcca tgacggaaga aattctgccg ccaaattctg ccccgaatcc tccagtgtaa
    52201 atgtcggcat tgcgaagaat atcggtgtcg aataccgaga acaaaccgat ggaatgaaag
    52261 ggattataga tgaccattcc atcgagaagg agtttgtttt gtataggtgc acccccacga
    52321 atatacagct gacctccctg gtcgccggtg aatacaaccc cgggtaagac ctgtagatat
    52381 tgtgcaaggt cgggttggcc tcctatactt ggaatttgct taatctggcg tggagtaacc
    52441 ttaaccaccg aggtgcgagt ttcggttcgc gcagcttccc tctctgccga aatactcacc
    52501 ccttccaaag ctatggactt tttggaaata taaagttttc gtgtgattat ttcccctttt
    52561 cttatggtaa tcggaaccac caaactatcg tatcctatgc ctgttaccat gagattatac
    52621 gagccggcag gaatattgtt gatgatgtaa taaccattta catcggtagc cgaaccaagg
    52681 gttgttttgc cgagaaaaac attaacaaaa atggcgggtt cgccactttc tttctcgtaa
    52741 attactcctc taatgcttgc ttcttgtgcc catgatttta atcccgaaaa tataaacaca
    52801 ccccaaatca tgcaacagga cgaaagaacg ataaatttcc tcataatatc attattatct
    52861 gacgtaagta aaattggatc acaaaggtat aaaaagagtc atacttcccc aattggggat
    52921 atgatgaatg gcttgataaa tgaggcaaaa acatcgcctg cataagagca aaactaaaag
    52981 tatatgaaat agatacataa tgaaagatag ggtttaaagt agaagagaaa atttgtctat
    53041 gtatagctca atcattatga agtctgattg taagttataa gcctttccct taaatgttgc
    53101 tgatcaataa aatcatatac cctgtcaaaa agtattgcaa accatactgt atagtgctta
    53161 ggagaagaag atagctcatt tttcaggaaa ttacgttcaa aatagtcaaa atcacttact
    53221 tcattgggat cgggttgtgg tggttcgtca gagattccta tataaacatg atcaaactca
    53281 tgttcagtca aaccattatc gagtttttca tgataaatga aatcgaatat tttaaaaacc
    53341 tcggcatgta ttcccatttc ttctttcagc ctgcgttgtg ctgctgtttt gttgtcttcg
    53401 ccagggaaag ggtgtgtaca tgccgtattt gaccataaac cagccgaatg atatttgttc
    53461 aatgctcgtt tctgtaaaag catctggcct ttcgaattga agattatcac ggaaaccgct
    53521 ctgtgcaata acgccttttc atgtgcttca atcttggcaa tttctccaat aattttatcg
    53581 tttatatcta ctaaaatcac tttatcgctc ataattgata gattagatac gtttgaaaaa
    53641 atacaaaata caaactttga ccattattaa atactttttg atgtttgaaa tccttattct
    53701 ttgtgagaga attttttcgg gtgaagagtg aataattttt ataaaaaggc tgttataata
    53761 gtaatatgca atggccacag ccagttttga acgaccaggt agttgtcgaa tgccagggaa
    53821 ggccactttg aagtcttgtt cgatagaatg ttcgattatt tttttgcttt gttggttcag
    53881 gctcatggta gacatctctg gaaagtagta tctgcctaag ttttgagtat ctgatttaat
    53941 atcgcgaagg aagtttacct tttgaaaagc cgaccctaat ttttgtgcag gcagttcgag
    54001 tttttgataa aggctcggtt cattatgaca aaataccctt aggcacatca atcccacaac
    54061 atcggccgaa ccatagatat attgactcaa atcttcctgc ttgtggtatt gagtaatatt
    54121 taaatctcgc ttcatgcttt cgagaaaagc attgatatgg ctgtgaggaa ttttgtattt
    54181 atttacggta tcagcaaatg ccatgagaac tgtgttagtg ctgatgttgt gaccgagcga
    54241 gtaataaaga tcttcggtaa gtttgttcaa aagaaaaatc ttgtcgtgtg gatggaaact
    54301 atctacgatc tcatcagcaa ggcgaacaaa tccataaatg gcataaattg ccgaacgctg
    54361 ttccttttcg agaaatgaca cggctaatga aaaggaggtg ctatatcttt tggtgatcag
    54421 ctccgaaatc ttgtaacaag tctgtaagta aaattcttta ttcataactt atatcttttc
    54481 ccatgattcc acttctccag ccactaaata aaacaatgga tgagttacag gcatgtcaat
    54541 aattttaact tcattgtatt ttttaacagc tcgcttttta agtttgttta aaaggtggtg
    54601 tagttgaaat tctacttgtc cttccgtaat tgctattcta gtcattgttt ccgtttgttt
    54661 gaatttagat acatcgaagt tataccctct attttgggct tcttgccata cgacatttaa
    54721 atagaagtca atacttttta atggcttcct tagctgtctt aaacgcaaca attgtggatg
    54781 attcctatag cctcgggtgt ttcccattag cacaattagg gctaataagg cttctttcca
    54841 taatgcaatt attcccttcg aatccaaata tttaggatgt attgatcaaa ttctcatttt
    54901 ttcatggttt gaatccaatt taaaatattc tttctacttc tttaaaaaaa tttagcttgt
    54961 aagatcaaat ggctgatgca attatatatt ccttcatgat tttatcctgt aggtatattg
    55021 ttctgcttat tgatcaacat ggatagaagt atttaggcaa atataaaaaa attgtccaaa
    55081 ctgtacaatc aaaggttatt ttgatgattt tgcctgttca tttcgatgaa atattcgatg
    55141 tttattattg ttgcaagtat atgttgctga cttttattca gtcatttgaa cctttagggt
    55201 tgtgatgatt ttatttttta tgatttgaaa ccttaatttt tataatttaa acttttagag
    55261 caaatcgatg agataatggg atgataagta tgcgcaaata actgataaat ttaggaatcg
    55321 ggattgtatc cacttttttt gaaaaattct tctggatttt tcatttcgat gatatccaat
    55381 accgcctgat gtttatccag acatttattg aaatatgctt ctactattct gtatcccata
    55441 aaatagccca aatcgggtgg taggtcattt tctactctat cgaaattata catccaatta
    55501 tcggtattta cacctgcttc gatatctttc ttgaatttca tccagagttc tttttcatga
    55561 gaaatgccat aatcatataa ataggcgttc cctctcactc cggttagcaa ccatgcaatg
    55621 aaatctgctg caccttcgaa aagacttcta ttcaatagat cataattatc gagccgagca
    55681 tttacttgca aatgatgaat tgtttcgtgg gcaatcacat atatgagcct cttgtctata
    55741 ttggtgtcgg aatcaagaat tttcccaaga gcatttaatc caaattctga tagatcgacc
    55801 ttgttgctgg atgtaattat ttcggctcca ataaataaaa attccctggt cgatgtacct
    55861 ccagctctta gcggactgat gacaaaacaa atttttagcg ggatataatt cgggtaatat
    55921 ttttcgattt tctggaataa ttgtatgatt tctgggtaga tttctttagt tagcagagta
    55981 tttgccctaa cactgttgaa aaatttggga taattacgga tggcttctaa ataatcttcg
    56041 gatgtataat cataaatgcg aatgaattcc ttaaaaccat cggttccatt atcgaataga
    56101 tattctttca agcagtaaat gctatccaat gatgaattac agtttttcaa catatcgaat
    56161 gccacccaaa agttgtcgat atcttttgta gtgacagtaa aagcgctatt ttcagcaggt
    56221 ttaatctgag aaaaagattg taaaccaaat aataaacaat ataaaaaaac aataattttc
    56281 aagaattttg gcatgatgca tagaattttt aatttgtcgc aactaattca agcagagttt
    56341 tttagctatt aaacctccta tatatccata gatttcgaaa ttatataatt tcgaaaatac
    56401 tatgtacgaa tcttcttctt ataacgcttc cgtcgaaatg atatttcatt caaattgtga
    56461 gtgataataa taaatttctt ccattaataa atatagagtt gaatttgatt gatttatttg
    56521 tgctttgccc tgttgccttt tactttttta tctcgacgta aattggaatt tcgacgctaa
    56581 acatcagggt gtaaagcgat atgcttttta aaaatcctcg tttttactca atgcttatct
    56641 cgatgttttc aatttgctaa atagggcctg atcttcataa aacccgattc atgtgtcatc
    56701 gatatgccaa gttaaacggt tggttttcag gataattttc ctcattttaa aatgttaaaa
    56761 aatttttatg aagcttcgcg cgatattcgc ttgttaaatc tattgtattt tagtgttaac
    56821 ttcatgttta ccccaatcct tttcgtaatt ttgcaggata gaaattctat tattattcat
    56881 gagtggagaa gctatcaggg tgcgatttgc gcccagcccg acagggccac ttcatatagg
    56941 aggtgtgcgt actgcattgt ataattttct ttttgcccgg caacataatg gaaaatttat
    57001 tctgaggata gaagataccg acaggaatcg ttttgtacct ggtgctgagg aatatattat
    57061 taaatccctt tcgtggtgcg gcattcaatt cgatgagggt gttcatctgg gtggaccttt
    57121 tgggccttac cgtcagagcg accgtaagca tctatacaaa caatacattg atatccttgt
    57181 cgaaaatgaa catgcctatt atgctttcga cagtcacgac gatttagaag aagctcatga
    57241 aaagaatccc aattttcagt atgatgcttt gagccgcatg aaaatgtgca attcgcttac
    57301 cttacccaaa gaagaagtca aaagaagaat agccgcaggt gagccttacg tcgtcagagc
    57361 tttgattccc atagacattg tcattagggt aaatgacctc attcgaggag aagtggaggt
    57421 ggaaagcaaa accctcgacg acaaggtgct gttcaaatcg gatggaatgc cgacctacca
    57481 tctggccaat atcgtagatg atcatctgat ggaaattacg catgtgattc gcggcgaaga
    57541 atggctacct tcggcacctc tgcatgtgtt gctatacgag ttttttggat gggagaaacc
    57601 gcaatttgct cacctgcctc ttttgctaag gcccgatggc aatggaaaac tcagtaagcg
    57661 cgacggcgac cgtctgggtt tcccggttta tccactcgaa tggaccgacc ctaaaacagg
    57721 tgaaaaatcg atgggatatc gtgaatcggg atattatcca gaggctttca tcaatatgtt
    57781 ggccttgctt ggctggaatc caggtaccga acaggaaatc ttttccctgg atgaactcat
    57841 cgatgccttt tcactggaaa gagtggggaa atcgggttca cgttttgatc ctgaaaaggc
    57901 caaatggttc aaccagcaat atttgagaaa acgttctgat gatgaattaa cccaggagtt
    57961 cattcccatt ttgaagaatc ttggcgtaga ttgttcttgg gaatatgtga aggaaagcat
    58021 acatctggtg cgtgaaagag taagttttgt gaaagaaatc tgggaccaag gatggttctt
    58081 ttttaaacct cctgaaaagt atgatgaaga agtcatcaag aaaagatgga aacccgaaac
    58141 ttcacagatt cttcaaaaat tttccgacac tcttttgcat ctcgatacat tcgatcaagt
    58201 ccatctcgat gaaacgatac aaaatttcct tcaggaaaac gctgttgaga tgggtgcttt
    58261 gatgagtcct ttgaggattt gtcttactgg aggtgctatt ggccctcatc ttaccgaaat
    58321 aatggccttg attggcaagg aagaaacctt acatagaatt gaaactgcac tgaaaaagat
    58381 aaaccattaa gtcatggctt ggattgccct cgaacaaatg gaattttggg cctaccatgg
    58441 ttgcttcacc gaggaacagg ttataggcac tcgcttcatg atagacctct atatagaaac
    58501 cgacacatca aaagccgaac tttccgacga cttaaacgac acaatcaatt atcagagtgt
    58561 gtacaaagta gtgaagaacg aaatgcaaat tccatcgaaa ctcctcgaac atcttggcag
    58621 aagaatcctt acaagtgtat tcacacgttt ccctaagatt tcttatgcta aaataaagat
    58681 atcgaaactg aatcccgcct tgggagggaa aattggcagt gtgagcgtta acctcgaaga
    58741 aaagagatga aagtggtttt ttgataaaga attagttatt cactcaatcg gccttgaaga
    58801 aaaatttatt ttagggctcc tgttttccgc actttttcgg gacaccgata taatatattg
    58861 ataatcaata tta
//
LOCUS       DAOORU010000002        51223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_741_length_51223_cov_5.987219, whole genome shotgun sequence.
ACCESSION   DAOORU010000002 DAOORU010000000
VERSION     DAOORU010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 51223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51223
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_741_length_51223_cov_5.987219"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1617
                     /locus_tag="PLF20_00280"
     CDS             <1..1617
                     /locus_tag="PLF20_00280"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOX79479.1"
                     /translation="PVFRTFLQHPYKYLNINNYFIILGVAKFFIMFIRKVKNRSGSQS
                     VQVIQKVRGNYKVVKTIGCATTQHEIEKLEQLAREEMERLSGNQLKLFGYESDEIIEQ
                     AFSLLDNSNIRTVGPELIFGKIYDYIGFNAVGEELFRHLVIARLAFPLSKLKTVDYLY
                     RYQGKSIEIDTVYRFLDKLSDKLKPQVEQIAFFHTLQILGGKVSVVFYDLTTLYFEAG
                     DEDDLRKIGFSKDGKHQCPQIYLGLLVGLGGYAIGYNIFEGNISEGHTLIPFLEEMSR
                     KFNLDKPIVVADAGLLSKANINALEANGYEYILGARVKNESERVKGKILATEFSDGMT
                     VSMKKSGNTRLVVNYSACRAKKDAYNRKRGLARLEKQIKSGKLTKSNINNRGYNKYLK
                     LSGDITIEIDYEKYDNDGRWDGIKGYLTNTKLSDKEVMENYRNLWQIEKVFRISKTDL
                     RIRPIYHRIRNRIESHICIAFTAYCILKELERVLYEEKSAISLKRATELTQNMYQITY
                     TLPESKQTKSKLLNMDEEQAELYRIVCKNF"
     gene            complement(2066..3904)
                     /gene="glmS"
                     /locus_tag="PLF20_00285"
     CDS             complement(2066..3904)
                     /gene="glmS"
                     /locus_tag="PLF20_00285"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016276365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOX79480.1"
                     /translation="MCGIVGYIGYREAEPILINGLKRLEYRGYDSAGIAILNEKLQVY
                     KTKGKVADLENLIDGKDLHGTIGMAHTRWATHGEPSDINAHPHFSQNDELALIHNGII
                     ENYTVIRDYLIQYGYQFRSNTDTEVLIQLIHYIKKTSGFNLVQAVQAALNHVIGAWAI
                     VVMDKNNPDTLIAARKGSPLVIGIGNDEFFLASDASPIIEFTHDVVFLNDEEIAIIQR
                     GENLRIRTIKDVEKNPLIQKLELNIHQLEKGGYEHFMLKEIFEQPDTIKDCMRGRISE
                     DNTTIAMAGIIDNIKRFMEARRIIITAAGTSWHAGLVGEYMLEDLAHIPVEVEYSSEF
                     RYRNPVIYPDDIVIAISQSGETADTIAAIQMAKQKGAFLYGICNVVGSSIARYVDAGS
                     YTHAGPEIGVASTKAFTAQVTVLALMALALGQKKGTISNQKVREVLTEMETIPDKVRQ
                     ILDKAEMIRDFSKVFTYARNFLYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMKH
                     GPITLIDQEMPVVVVATNRGTYEKVISNIEEVKSRKGRIISVITEGDTRVRKLSDYYI
                     EIPETEEMLVPILATIPLQLMAYYIAVLRGCNVDQPRNLAKSVTVE"
     gene            complement(3944..5281)
                     /locus_tag="PLF20_00290"
     CDS             complement(3944..5281)
                     /locus_tag="PLF20_00290"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HOX79481.1"
                     /translation="MKQIIQKTCWFVFLAFLAGSCTKEPDQIGTSIIPPTDKLQYGID
                     TTILLDAYSIVEDSVRTDETSQNILGSYWDPIFGISTAGIYTQLSLPFVGHSFGNNPI
                     IDSAILKLAIKGAYGDSTSLLTVNVYELLDTISYNSIYYSNNSIEHSTVPLTSKSFIA
                     RPYDSVYIDSVKYAPHLRIRLDQSAPDFIQKIFSTPSDALSQNTEFIQYLKGLYITAE
                     DATMPGKGSLLYVNLESALTELILYYKNDESDSLSFSLVVNSATARFNHFDHYGYAHA
                     DPLFKQQVLYNDKTLGKERLYLQTMGGIKTVVKIPDLKGLVADGQKIAITEAKLIISN
                     ADANSIYSAPDNLVLFEEDTTGQVYFLSEQSIGTEYFGGIYNSNQQNYYFRIPRYIQD
                     IFDGTRDSTRHLTLIASSPSVRGNRLVFGGTNPTNPSLYEKRLRLQIVYTFLP"
     gene            complement(5347..6165)
                     /locus_tag="PLF20_00295"
     CDS             complement(5347..6165)
                     /locus_tag="PLF20_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOX79482.1"
                     /translation="MTKARVLFVSQEMVPYLKDSPMGQISRYLPQGIQEKGKEIRTFM
                     PRYGHINERRNQLHEVIRLSGINIIIDNTDRPLIIKVASIQQARMQVYFIDNDDYFAR
                     KFQFYDKNDKFFKDNDERAVFFARGVLETVKKLGWNPDIIHCHGWFTSLLPLYVKTAY
                     KDNPIYTDAKIIVSLYDDDFEGEMGKTIKSKIKLPGIREKHLANYKKGTYIGFMQGAL
                     DFADGAIIASENVNPEILSYLDQKNIPVLPLQDEETYIDAYNDFYEKILQNHNS"
     gene            complement(6452..7177)
                     /locus_tag="PLF20_00300"
     CDS             complement(6452..7177)
                     /locus_tag="PLF20_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HOX79483.1"
                     /translation="MRTPAILLHYIHYTDHSIIARLYTRQGGIKAFMIKGINKKNPSL
                     KPSQLLPLSLLEIVYEEKPHNRIFFLKEARNYFLSYKVQNHVIKAGITLFMAEILNHS
                     IKEEEANILLYDFLEKTIIRLETQEENVAFYPIWFLIRLSSFLGITPLDNYSSETITF
                     NMEEGKFQENVPERNTYVAGENVKTLSLLIKAFSSSDYFIPNIHIKDKTAIIDILIRY
                     YHQHLPGFGEIHTHKILKEVLNS"
     gene            complement(7334..8410)
                     /gene="buk"
                     /locus_tag="PLF20_00305"
     CDS             complement(7334..8410)
                     /gene="buk"
                     /locus_tag="PLF20_00305"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOX79484.1"
                     /translation="MNNERILAINPGSTSTKIAVYEGNKNIFLKTIRHTPEELQCYRK
                     ITDQFSFRKDIILKELKAAGIDLNSIRVVVGRGGLVKPIPSGIYEVNSRMIEDLKAGL
                     MGEHASNLGGIIADEIARSLKDAKAYIADPVVVDELQDVARIAGHPLFKRVSIFHALN
                     QKAVARLYARSQNKNYEDLNLLIAHLGGGISVGAHQKGKVIDVNNALDGEGPFSPERS
                     GTLPVGQLAALCFSGEYTLEEVKKMITGKGGYMAYLNTNNAYEVEIAAREGNQKAALI
                     QDALAYQVAKEIGAMASVLKGNVDAIILTGGIAHNTYVVNYIKEMIGFIAPIVVYPGE
                     DEMSALAINGLMVLRNEVNAGIYE"
     gene            complement(8403..9326)
                     /locus_tag="PLF20_00310"
     CDS             complement(8403..9326)
                     /locus_tag="PLF20_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOX79485.1"
                     /translation="MITNFTQLVDKLNTAAPQTIAVVAAEDHATMGAIVRAIRTGFAK
                     AILIGNKTRTESLLAHLELPPETFLIVHLPDEQMAMKEALSLIHQGKADILMKGLIGT
                     DIFLKAVLDKTSGLMKQGEVMTYVAALQIPSYHKLLFISDTAVIPYPNLEQKIAMLKY
                     AVEMARRFGISKPKVALIGASEKVGNQSQTAIDASIICKMAQRGQLGEVIVDGPLDIF
                     LATQPESLNIKRIITPIGGEADILIFPSLESCNAFYKGLVRFAGAELAGLLQGTRKPV
                     VLMSRSESENSKFYCLALACLMANNNNLSYE"
     gene            complement(9354..10256)
                     /locus_tag="PLF20_00315"
     CDS             complement(9354..10256)
                     /locus_tag="PLF20_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOX79486.1"
                     /translation="MITHLEQMIEAAKAKARKRIVVACANDNHSIEAVHHAMELGIVD
                     ATLVGDEATIVKVCKDLNIQPEKFRIVQESDEQKAAHKAVELIVKGEGDILMKGLVTT
                     DKYMRAILNKDAGLLPPKAILSHVTVIENPNYEKLLIVGDVAIIPVPDLDQKIAITNY
                     LIQTAHRLGNEKPKVAIIAATEQMLPNMPTCVDAAIISKMGERGQIKGALIEGPLALD
                     TAIDKESAAIKKLKSEVAGDADCLLFPNIETGNVFYKFNTKLAKGELGAIVIGAKCPA
                     VLSSRGDSALTKTYSIALAALSTD"
     gene            complement(10282..11577)
                     /locus_tag="PLF20_00320"
     CDS             complement(10282..11577)
                     /locus_tag="PLF20_00320"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOX79487.1"
                     /translation="MAHFFHQHLLSILFVLSWLVHPNVIHAQFDSLFLNKTLRFDYLH
                     IGDARNEFFALDELLEEPYWAGSHLHLIDTFGYGKFQCRLYDQATGELIYSRGYSTLF
                     SEWQTTLEAQNVYRAFEESVVMPFPRHDAFIEIYSRNRKGQFEKKFSYPFRVNDYFVN
                     QKQRMVFPVFKIREVAPAEKALDIVILPEGYTSQQMEIFKQDCQKFAEILLGYSPYNE
                     YSDKINIKAVLAPSFEESSDFPPSGIWKNTLLHSKFYTFDLDRYCMTDAFQTVRDVAS
                     NAPYDQIYILVNSSMYGGGAIFNYYALSVNSNMLAGKVFIHEFGHSFAGLADEYYDSE
                     VAYNEFYPLDLEPQEPNITTLVNFKRKWADMIEEGTPIPTPAEEIYRNKVGVFEGGGY
                     AAKGIYRPAMDCLMNTFRQNTFCPVCIRAIRKMLDFYTQ"
     gene            11967..13349
                     /locus_tag="PLF20_00325"
     CDS             11967..13349
                     /locus_tag="PLF20_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOX79488.1"
                     /translation="MELPFAEYYRIKMVEPIYPSTREQREDWIRQARYNLFNLKSEQV
                     YIDLITDSGTGAMSDRQWSELMLGDESYAGASSYYKLKQSIGEIFGFPYFLPTHQGRA
                     AENVLFSALVKAGDVIPGNSPFDTTKGHIEFRNATAIDCSINEAFDTSISHPFKGNVD
                     LQKLEDVLKANPREKIPFVLITVTCNTSGGQPVSMQNLRKVKALAAKYGIPVCYDSAR
                     FAENAYFIKKREPEYQDKSIREIVTEMYSYADLATMSGKKDGIVNIGGFIGFRSEEIF
                     RQATTFNIMFEGYITYGGMSGRDMNAMAEGLRESTTFEYLETRIRQVEYLGKRLTEFG
                     IPIQQPVGGHAVFVDALKFLPKVPRKEYVAQTLAVELYLEAGIRAVEIGTLLADRDPV
                     TREDRYPALETVRLAIPRRVYTNNHIDVVAVAIKNIWDRRMDIQHGYRITWEAPIMRH
                     FTVELEKSDN"
     gene            13957..17181
                     /locus_tag="PLF20_00330"
     CDS             13957..17181
                     /locus_tag="PLF20_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX79489.1"
                     /translation="MYKITTHPILEVPKAEKVTFRYDGHIIEAEKGYTIAAALHQAGF
                     PVHSHSLRNRKRSLECGIGKCGACEMLVNGQVKRICITKVDGVHEVKEIPHDYRPGVV
                     EYRKNEPIDVYKTQVAIIGAGPAGLAAREILCQFGIDNLVVDNNSKIGGQFLMQTHQF
                     FFFEKEKKFGGMRGFDIAQTLAGTDHDGIFLNSTVWDILEGGRLAVKEITTDRIYYID
                     AEYLVVSTGAVPFMPSFENDDLPGVYTAAVVQKMMNMQFTLLGKNVLTVGAGNIGYLT
                     SYQLMQAGARVKVILEAQPFEGGFPVQANRVRRLGIPVLTSHILLKAIPNRDLTGITG
                     AVIALCKNFHPVVGTETVVEGIDAINICTGLVSDDQLLIKGNEVFGRKCYGAGDTIRI
                     GEGTSAVLRGKQVAYEILMEMGIRLNYDDYLSLSKEYIDSQQHPIRIIDRPYMPDHER
                     MQAKGFVLIDCLYGFACNPCAFACPQGAITKSSTSTVPSIDFDKCIGCMDCVYQCPGL
                     AIFGYNLKRDWVFLPIEYDVKENAEVFLVDNYGKILGEGVIDKILRKPNKTNIARVKS
                     LSLHGEDLTQVRSFIVKKNYPVPLELRPYDEQIPVESYICHCDDVKIEEILEVVGERK
                     FISIDEIKHTTRLGMGACRGKRCIPRLKSKIKDYGIQIVGEATPRGPMSNQLSLGELY
                     PRPVHENFVVNRSVRKEKVETLIAGGGMGGSALFRYLSEAGKKPVLINFGRGSSWRNI
                     AGGRPNFSVPELSDIAEHNRQIFIELQQLHNIDYHPIQYVNFAHDEEMYKALEAAMAW
                     SKAEMISPKDFTKRISPFFNPGLKTYIAAQITYNCWQATPGKTIDLIRRIGIQQGGTI
                     LEDCKLIDVRKNGQEYQVLVQDHTKQYIEYSSEIFINALGPEAAYFARLLGIETGLYP
                     VRHQAFITRRMPMLGVNDIPLPMLIDRRKYKGFVAVYGQQLAETGQVIGCASPAVDPA
                     ETLKNLKVNSREFLEIFSEVFVDWLPELSSVGFQAVWSGYYVEPRMIIDPDLGLFVGL
                     RGQGFMLSQYLAKMYVDKLTGRPVPEYFSRLSIQGDGLPEKAFK"
     gene            17264..17788
                     /locus_tag="PLF20_00335"
     CDS             17264..17788
                     /locus_tag="PLF20_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HOX79490.1"
                     /translation="MLNALKPYHLGFSGLALGHHHYDFDIGSKFFDCFEESEIHAGKV
                     KVDLDLEKQKKMLVLHFDIHGAVEVVCDRCAYEYMQPIAGKEEVYIKFGPEYIEEDDD
                     VLVLPQGSHEVDISQMLYDFIHLMVPYHRLHLPDENGNPECNKENLEILERLAPKENQ
                     IDPRWEVLNRLKTD"
     gene            17821..18006
                     /gene="rpmF"
                     /locus_tag="PLF20_00340"
     CDS             17821..18006
                     /gene="rpmF"
                     /locus_tag="PLF20_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOX79491.1"
                     /translation="MPNPKRRWSKTRTRKRRTHYKAKMPAAFACPNCGSPVLYHRVCP
                     SCGFYRGRQVMEVEKMK"
     gene            18145..19146
                     /locus_tag="PLF20_00345"
     CDS             18145..19146
                     /locus_tag="PLF20_00345"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOX79492.1"
                     /translation="MTKIRAAITGVGAYVPDYILTNEEISRMVDTSDEWIMTRVGIKT
                     RRILRGKGKGTSDLGTPAVNELLRKTGTKPEEIDLLICSTVTPDHLFPSTSAIIADKV
                     RIKNAFCFDLNAACSSFLIGLSSAAKFVESGRYKKVVLVGADKMSSITDYTDRSTCPL
                     FGDAAGAVLIEPTADTEVGYIDEILHTDGYGRIYLYQKAGGSVYPPTHRTVEAREHYI
                     YQEGQVVFKAAVASMADVAVEMMEKHGLTSETIHWLVPHQANNRIIEAVARRMRLPKK
                     KVMINIDKYGNTTSATLPLCLYEWEHQMHKGDNIILATFGAGFTWGAIYLKWAYDPR"
     gene            19262..20062
                     /locus_tag="PLF20_00350"
     CDS             19262..20062
                     /locus_tag="PLF20_00350"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOX79493.1"
                     /translation="MKKLYLAFISCLFIVVLNSCSKDDTSINIFTIEDDIALGEQVSN
                     EIAANPQEYPILDPVKYASAYDHLYRIRNTILASGKVKYATKFRWNCKIIRNDTMINA
                     FSLPGGDMYFYTGIIKFLDNEAQFAGVMAHEMAHADRRHSTDQLTKVYGLEILLGVLL
                     GSNPNFAEQIAAELALGLGTLAFSRSQEYEADEYAVKFLYATEYDAASLGDFFEKMEG
                     EPYPPTFLSTHPSPEDRLAKIEEHFQALGGMHGATFAERYKEFKNALP"
     gene            complement(20066..20890)
                     /locus_tag="PLF20_00355"
     CDS             complement(20066..20890)
                     /locus_tag="PLF20_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HOX79494.1"
                     /translation="MYCYHTHTYFCDGTSAPELYVKNALQLHFYSLGFSGHAPVPFAN
                     NFAIPNEKLISYCEEINSLKSKYQNKLSILLGLEMDYIPGVSKPFEELARECHLDYTI
                     GSVHFVRNVAKEELWFIDGPKREIWDKGLEEVMEGDVKWGVNLYYRQIQEMIEREKTD
                     IVGHLDKIMMYNAHRYFSENEPWYRYLVIETLMKVQQCGAILEINTRGLYKKLSDQLF
                     PSETFLPVIKELGIPVVISADAHHPSEIGLLFSQTLDLLLSKGFEEINHKGFKCYN"
     gene            complement(20902..22596)
                     /gene="ispG"
                     /locus_tag="PLF20_00360"
     CDS             complement(20902..22596)
                     /gene="ispG"
                     /locus_tag="PLF20_00360"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007479874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HOX79495.1"
                     /translation="MEKYPFYIQKDYQRLTTIEIPVGDIPVGGTNPIRIQSMTNTFTM
                     DTEATVAQAIRLFDAGCDYVRITAQGPKEASNLKAIKEELHLRGYTKPLIADIHYSPK
                     AAEIAAAVVEKVRINPGNYIGPHMGKITYTDKEYREELEIIYDHIKPLLEICRQYGTA
                     LRIGSNHGSLSKRILSRYGDTPEGMVASAIEFIEICEDLDFDHIVLSMKSSNPRIMIE
                     ANRLLAMTMLKRGKVYPVHLGVTEAGDGEDGRIKSAAGIGALLAEGIGDTIRVSLTEE
                     PEAEIPVASILKEMTSVFPLNPLAFGLKNYRYENFQKEKFVSNEIHEIGGMNPPLVIG
                     QTSSADIVILSDLPFRTHQIHTQSFPSHLEIARLQEELPIVIHSDHPRELIALRAAVI
                     HLRNQGMNQPIIIKRSSYEAKPEKFITESAAVMGPAFQEGLGQAIWLENPKLKPEQLV
                     NISFSILQSVKARISRTEYIACPSCGRTLFNIQPTLRSIKERTSHLKGLTIAVMGCIV
                     NGPGEMAGADYGYVGAGRGKITLYKGHAPVKRNIPEEMAVDELIDLIRQNGDWIEP"
     gene            22876..23751
                     /locus_tag="PLF20_00365"
     CDS             22876..23751
                     /locus_tag="PLF20_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HOX79496.1"
                     /translation="MPRPNASELVLSADGNIYHLRIKPEQLADTVILVGDPGRVSMVS
                     SHFDRLEFQGQNREILVHTGWIGKTGISVISTGMGPDNIDIVVNELDALVNIDLTTFE
                     EKPQKTFLRLIRIGTSGSLQPDIPVDAFVAAEYAIGLDTLLRFYRPEGNSFDEEIAEE
                     FIRQTQWKLDMGKPYVVRGSSELLSLMGEEVVRGITATAPGFYGPQGRKLRLPLAMPD
                     MDERLMRFRYRDRCITNFEMETSALYGLSALMGHQALTICLTIANRATGKFSPDYRSR
                     MNELIRLVLEKMTHS"
     gene            23762..24907
                     /locus_tag="PLF20_00370"
     CDS             23762..24907
                     /locus_tag="PLF20_00370"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOX79497.1"
                     /translation="MNLYLNSPPPFCQPEVLLDIPKCKANIEKMVNISRQEGIIFRPH
                     FKTHQSKGIGRFFRQAGVSAITVSSVSMAKYFAEDGWNDITIAFPVNLREWEQIKSLA
                     SIVHLSLLISDETSLQKISRLDFLSFHLNFFIELDTGQGRSGFEYSAEKILPLIRLAD
                     ELQIPIEGFLFHDGHTYKAHNTDEIRKIRAATLAMTAHLRHDLEVFYPGRKFLYSMGD
                     TPSCSIDNRYEGIDEMRPGNFVFYDLMQYDLGTCTQDEIAIRLVCPIVAVYSEQERAV
                     IYGGAIHLGKDFLTLPHNEICFGAICAHGDSIEWQGRYPMAWLSSLSQEHGVVKAPKD
                     FLKKIQPGDLLDVVPVHSCLMVSAALRIIGSDGTIFDTFNANFMKDT"
     gene            24949..25830
                     /locus_tag="PLF20_00375"
     CDS             24949..25830
                     /locus_tag="PLF20_00375"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOX79498.1"
                     /translation="MEKKETIKELIALISMVDEPDEVVYGRIRQRIIDLGEEALPSLE
                     KAWENSFEEFTRLRIQELLDTIHFESIQQQLITWVNSNQDDLLEGAILLSRYHFHELD
                     INRITHNLGQIIQDVWLEMNDRMNPLEKIKVLNHILFDVHKFTPTKPPQYSLQDYLIP
                     NLIDSKRGSGTTLGILYLIIAQSLKLPVYGVNLPYNMIIAYLENNPGIYIYDPENDPV
                     LFYINPVFKGMIFIRKEIDRFLKDNKIEPQPQYFTACNNVQILQRLASEMRMMYDMEG
                     DSEKAERMDQIIAILKS"
     gene            25946..28717
                     /gene="polA"
                     /locus_tag="PLF20_00380"
     CDS             25946..28717
                     /gene="polA"
                     /locus_tag="PLF20_00380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOX79499.1"
                     /translation="MPEQKLFLLDAMALIYRAFYAMVKTPRINSKGMNTGAVLGFANT
                     LWDVLKNENPTHIGVAFDMQAPTIRHEDFADYKANRQAMPEDLAASIPWIKELLRAFN
                     IPILEQEGYEADDIIGTLAVQAAEKGFKVYLMSTDKDLGQLVNENILLYRPARFGNGA
                     EILGMNEICQKYGIKNPTQLADLLGLWGDASDNIPGVPGIGETYAKKLIAQFDTIENL
                     IQNADKIENPKLKKKILSHTEEALFSKRLATIRLDAPVKFDEELFKRQAPDINTLKKI
                     LDFLEFRAFSQRIFKDLNHDKPSEQLSFFPEETSSRHESDTNLFKNIHTTPHQYLTLT
                     SLEEIRSFADTLQKSSEFCFDTETTGIDPNNCDLVGMSFAVVAHRAWYIPMPVQRDEI
                     LDILQILKPVFHNRKILKIGQNLKFDLIVLQWYGCNIYGPFFDTMLAHYVIEPEQRHS
                     MDYLARAYLNYDPVPIESLIGKKGNNQRNMQAVELETIKEYAGEDADITLQLKHAIEP
                     LLHQTSTYELFQEIEMPLMPVLVSMEIEGVKIDTEALDAYGKQLENEIHRLEDEIYSL
                     AGESFNISSPKQLGIILFEKLKISENPRLTSTKQYATGEDVLEKLAHRHPIVEKILDY
                     RELTKLKSTYVDTLPQMINPRTQRVHTSYNQAVTATGRLSSNNPNLQNIPVRTERGRE
                     IRRAFIPRNPDYILISADYSQIELRIIAHLSGDEAMKEAFRQGLDIHAATASRIFNIP
                     LDQVTKEQRRTAKSVNFGIVYGISAYGLSQNLDIAKKEAAAIIEEYFRQYPGIKAYME
                     KSIAFAREHGYVETIKGRKRYLPDINSANANIRGFAERNAINAPIQGSAADMIKIAMI
                     RIHQALSEKFPHSYMILQVHDELVFDARKQEAEEIKIMVKDIMQNAMPLSIPVVVDIN
                     AGENWLEAH"
     gene            29083..29424
                     /locus_tag="PLF20_00385"
     CDS             29083..29424
                     /locus_tag="PLF20_00385"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOX79500.1"
                     /translation="MFKSNNQQEIFSFEDELNQKQGDLLNSSKGKWFYHILFSNINEL
                     DFRDLYSQKASRPNVPGNVLVCALILKELKGISYDELIEGVVFDLHFKTALGLSWIGD
                     IPFSRATLFNF"
     gene            29706..30230
                     /locus_tag="PLF20_00390"
     CDS             29706..30230
                     /locus_tag="PLF20_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79501.1"
                     /translation="MKSSELPHELEKLGKIYYTIFEWLSKKEEYHSRKEYINFTRAYN
                     EHFVVKGEEVNPRSEKELTRSMLQSPDDVDATYRKKNGEQSKGFSINITETANPDNPI
                     QLITGIVVMPNNVDDSQILNERIDIIKEKTPQLNEMHIDGDFGSEENDKKFEELGINH
                     ITTAVRGREREIEI"
     gene            30456..30839
                     /locus_tag="PLF20_00395"
     CDS             30456..30839
                     /locus_tag="PLF20_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79502.1"
                     /translation="MSIPKERWKLRPNIEATAREFKIRTEDGKLKVRGLIKASLFAYS
                     SGIAINFGRTYRFLVKNGCPDGFNRPIIVFLKDIMSNTGEILTKCEFIVDNFSLFRYF
                     WFFLIQKIFSGPKMSKQETLIQLSF"
     gene            31144..32232
                     /locus_tag="PLF20_00400"
     CDS             31144..32232
                     /locus_tag="PLF20_00400"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOX79503.1"
                     /translation="MLNALRLQFSLRNGKPMALLLIAVILSASLLKSQPPAGYYDGTE
                     GLTGVALQAKLHDIIDDHASVSYGSILLHFQTTDKKPNNTVWDMYSDVPGGNPPYIYY
                     FNEDECGSYKSEGDCFNREHSWPASWFNDLIPMYTDLFHIYPTDGYVNGKRSNYPYGE
                     VGSASWTSMNGSKLGNCNYPGYSQVVFEPIDEYKGDLARSYFYVATRYYNEDNGWASN
                     GMVTGSQLKPWAVSMLLEWHHQDPVSQKEIDRNNAVYNIQNNRNPYIDQPDFVDKIWG
                     EGAGIAHEISVIYQLYPNPCFNFIKIVLPDDPTDSYVFVLTDIAGRRIFSTSLRGGKE
                     NLIDVSHLKDGIYIGQIDENIPIRIIKQ"
     gene            32233..32688
                     /locus_tag="PLF20_00405"
     CDS             32233..32688
                     /locus_tag="PLF20_00405"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOX79504.1"
                     /translation="MNYIRITKKFRFEMAHALVGHDGPCRHIHGHSYELCITLKGQPK
                     NDPRSSKNGMVMDFGELNRIVKSALIDRFDHALVLNDAVRDEIPLGGRELFARTIWVE
                     FQPTTENLLLYFVEILRPLLPSDIELYSLKLSETATSYAEWYAPDQKAD"
     gene            complement(32693..33688)
                     /locus_tag="PLF20_00410"
     CDS             complement(32693..33688)
                     /locus_tag="PLF20_00410"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOX79505.1"
                     /translation="MKIGKYFGMLCMSLIFLYSCKGPQHEKKPKIAIDTTFYPSFSAD
                     TAFSFVVKQVGFGPRVSNSDANRRCGDWLIQKLGSYSSDIIVQAFKARAYNGEVINGR
                     NIIASFRPEASSRILLASHWDSRPFADHDPNPANRHKPIDGANDGASGVGVLLELARI
                     MHSYPPNIGVDIILFDAEDYGPPEDMQSQQGEDWWGLGSQYWSRNKHIPSYNARYGVL
                     LDMVGVPQPSFLMEGFSMYYAPDVVKKIWDIAASLGYGKYFPKQKGGFITDDHYYVNK
                     YAGIPMIDIIHLENDSQGSSFFEHWHTLQDNLEHVDSQSLQMVGDVLIRLIYKPQ"
     gene            complement(33713..34285)
                     /locus_tag="PLF20_00415"
     CDS             complement(33713..34285)
                     /locus_tag="PLF20_00415"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOX79506.1"
                     /translation="MEILDILGRRWSPRCFDDKAVEQEKLQNMMEAARLAPSAMNEQP
                     WYYFIGRKDDETYRKIFSTLVEFNQLWAIQAPLLMMACGRTKYVSRDNSDNRYWDYDV
                     GQSVAYLTLQATYEGLFCHQMGGFDTDKTAELFHLPEEVAPIVVIAVGYMGNPETLPD
                     FLRTMENGPKTRKSLESFIFGSDFGIPYQF"
     gene            34447..34896
                     /locus_tag="PLF20_00420"
     CDS             34447..34896
                     /locus_tag="PLF20_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79507.1"
                     /translation="MKKLFCVILIIAAGFMHAELLQAQRNYGSYELGLRLGDTYGGSA
                     AVDAMLPLAGNRLHADLSFHHDGLILAGLYDWGFPIAPGFIFYPGVGGIVYIGDVFRL
                     GVTGELGFEYAFDIPLSIGLDWRPIITIIDEGGFSGDGFGLNIRYRF"
     gene            35374..36339
                     /locus_tag="PLF20_00425"
     CDS             35374..36339
                     /locus_tag="PLF20_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79508.1"
                     /translation="MKKMTFILGLAIASIIFLAPQKKICAQTLEDAGNTFNSAIQLMQ
                     NDLPSAISKFEEVIGMCEKIGESGNELKMQAQNQLPILYYNYAVNLQASQNYDQAIEA
                     CNKAIQTGQQVNNANIIAKAVNLTAQIYMVKGNEYFKNKDFANALVSFDQAITLDSTL
                     YRAYYGKLLVYREQDKYDEMDEMLEKVKTTSTQDPQFAEKAQALVAREYSIAAAKAIQ
                     AKNSSLALTYCDKALALGDATANTYYYQTLAANMEKKYSLAAEAAQKALELEQKDKSK
                     IYFELGKAYEGLGRAADACAAYKNVTDGPLVQNAQYQIKQVLKCK"
     gene            complement(36485..37465)
                     /locus_tag="PLF20_00430"
     CDS             complement(36485..37465)
                     /locus_tag="PLF20_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOX79509.1"
                     /translation="MNPYDIFNIDNEDEFNQMALSIYHFQKEHNKVYREFLKASHHFD
                     IPIHHYSEIICLPIEFFRTQKVIVEQQSPQMVFESSGTTGQNVSRHFVVDVELYRQSF
                     LRGFAQFYGNPSDWTFLTLLPSYRGRPNSSLIFMMKGLMEVSESPYNGFFLDEVEKLS
                     ERVEKAKTWREGKVLLMGVSHALWDLAEKFPQNLQGVIVMETGGMKGRRKEITRSQLH
                     KILSHSFEMEKIHSEYGMTEMLSQAYSQGEGIFKTPPWLKILLTEADDPFSLTKPGRS
                     GLINIVDLANYYSCSFIATRDLGRSVGIEQFEVIGRHDLAESRGCNLMVW"
     gene            complement(37589..39106)
                     /gene="hutH"
                     /locus_tag="PLF20_00435"
     CDS             complement(37589..39106)
                     /gene="hutH"
                     /locus_tag="PLF20_00435"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOX79510.1"
                     /translation="MPVYQISSKELTYENIQHLMEEGWQIALSDEAIHKVEHCRDYLD
                     NKLDMHAGPVYGINTGFGSLCEIEISKEQLKDLQRNLVMSHACGTGDEVSEQIVKLML
                     LLKIQNLSYGHSGVQLDTVKLLVEFFNKGIYPVVYEQGSLGASGDLAPLAHLSLPLLG
                     LGEVTYKGEKMPASKALFEAGLSPVDLKSKEGLALLNGTQFMGAYGVYCLLRSFRLST
                     LADLIGALSLDAFDGRIEPFYLPLHEIRNHKGQISTAKGIANLLESSELMNKPKNHVQ
                     DPYSFRCIPQVHGASKDAIEYVAKVFSCEMNAVTDNPTIFPYEDLIISGGNFHGQPLA
                     LALDFLAIALSELGNISERRTYQLISGKRNLPHFLVVNPGLHSGFMIPQYTAASIVNQ
                     NKQLCTPSSVDSIESSQGQEDHVSMGANAATKALKVVNNLQRILAIELFNAAQALEFR
                     RPAKSSPLIENFVHKYRQYVPFIQYDEVMHPHIENSIRFLSEVKLPISDLFIPNL"
     gene            39583..40251
                     /locus_tag="PLF20_00440"
     CDS             39583..40251
                     /locus_tag="PLF20_00440"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOX79511.1"
                     /translation="MFQKYIRRFGIGILWKSIMRSFFVFILLGFMLSTYSGVRAQKVY
                     GGLVAGVNGSQVAGDVASGYDKAGIHVGLFSYTHISEKSVFQMELVYSQKGARKNPSE
                     KNGYQQYILRLNYIDIPFIYKYLYNDRLSFEGGLSYGYLLHHYEEANFSLNVGDAGFH
                     THALNILVGLNYRFAEKWAAGFRSTNSLYQIRDHTSGASRFPNYGQYNDVLTLTIQYL
                     LWRE"
     gene            40531..42057
                     /locus_tag="PLF20_00445"
     CDS             40531..42057
                     /locus_tag="PLF20_00445"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HOX79512.1"
                     /translation="MESLIVNGEGLTIEDIVAVARHHQKVVLHPEAIEKIVQCRAMLE
                     KKIEAREIMYGVNTGIGEFSEVVLTDDQVKDFQKYLIYNHAAGIGEPMPEEWVRGAML
                     GRINVHAHGNSGCRPEITLTMVEMLNKGVTPYVCTKGSVGACGDLAPMAQIALLLMGE
                     GKAYYEGELLDGKEAMRRAGIEVPGLKARDGLAAINGSNVLTAMSAIFLYDANRWLKQ
                     AEIATAMSLEALKANLKPYHELIHQVRGFPGAMRSARAIRKMVEGGDLAEGRLKCKVQ
                     DAYSMRSTPQVIGAAHDALAYARSQVEIELNGVGDNPIFFPELNLQLSGANFQGSPVS
                     LPMDMAGAAITMVCVLSERRLNRLNNPALSVGLPAFLTKGAGMFSGLMLSQYTADMQI
                     VEQRILSMPASIQSIPAAADQEDFVSMGMNTALKNFQIIDNAYGILGIEMMAAAQALD
                     FRDYKFGTGTTCAKEIVRQYVEFLDVDRPLFNDHNTMKKLVESCEILEKVENKIGSLS
                     "
     gene            42072..42500
                     /locus_tag="PLF20_00450"
     CDS             42072..42500
                     /locus_tag="PLF20_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79513.1"
                     /translation="MEIRENNTPSQEKRPKILTILCILSFIGSGMGMIAYLFYGLMFN
                     EVETMIEEGQFAFPGIEILMSGGNKYFLMGSLLYLISFAGVLFMWKMRAIGFHLYTAA
                     QILMLILPFLALENYPVPFVDFIITLIFILLYRKFLPMMN"
     gene            42761..46054
                     /locus_tag="PLF20_00455"
     CDS             42761..46054
                     /locus_tag="PLF20_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOX79514.1"
                     /translation="MRKIPFLLAFLALLGLQVQAQRQVTGTVTNAEDGSSIPGVSVLV
                     KGTQVGTITDINGKYSIKVPLEGKILQFSFVGMQTLEVTIGNQSVIDVQMRPTTTALE
                     GVIVTALGISREKKSLGYAVQDVSGDEISQARQLNAINSLSGRVAGLQITSGSGNMGG
                     SSRVLIRGVNSISGNNNPLYVIDGVPIDNSDFNTVNTARGAGGVDYGNMAQDVNSDDI
                     ESISVLKGPAAAALYGSRAANGVILITTKKGKAGAAKGIGVSINSGISIENVAYLPKY
                     QNLYGGGTTFTGAGTKDGFLVQEINGIEYLLPDYATDESWGPRYNPNIYYLPYWSLYD
                     WEALGKPTTNADGTPYRLETAPWVAPKHDVEDFFETSVGYTNNIALTSASENGSFRLS
                     YTNFSSNGYMPNSTLNRNTFNFNGTSKFGKKLMAFTSFNYIKTYALGRPVTGYDDNNV
                     LQKFTQWGQRQLDMEKMKNYMNPDGTQRTWNRRAWFDPLPNYSDNFYWTRFMNYEEDW
                     RDRYYGNVGLTYQIFDWLKLTGKYNMDTYTFRINERVAIGSQALSYYQEDVRQLTETN
                     AEFLLEADKRFGDDFTTKLTLGGNRMHRQYELNGGITQGGLVIRNFYNLRNCLSPALV
                     HDETSQKSINSLYGSLNLGYKGLLYLDVTLRNDWSSTLPKNNWSYPYPSVTLSYVFSE
                     MPGLQNQNWFDFGKLRFGWAEVGNDTEPYRLITLYENIQNFGSDIIYTLPGMLNNAKL
                     RPESTNGWEIGTELRFLNNRIGLDFTYYYKKTTDQIFNTPISAASGFTSAMINAGSIS
                     NKGIEFLLTLTPVKVKDFTWDILLNFAKNTNKVEELIEGVETLRLANGPFNISVEARV
                     GEAYGQLVGTDFVYDAKGNKVVGTNGRYLKTTVVQSLGSVLPDWNAGITNAFKFKGFD
                     FSALIDIQHGGKIFSTSYMWGIYSGMLEETAAINANGKNIRDAVADGGGVLIDGVYGK
                     LNADGSVTYLDKDGNPVDSPVKNETYISGVRWAADHYSGPAAQNVFDASYVKLRELRL
                     GYTIPNRYTGPIQNLRISIYGRNLAIWGRDNKHIDPEYTTSSGNTQGIEGGQLPSLRS
                     FGINLTCNF"
     gene            46086..47624
                     /locus_tag="PLF20_00460"
     CDS             46086..47624
                     /locus_tag="PLF20_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HOX79515.1"
                     /translation="MKTLHKIAVVFNLPLFILAGCTKDFEEINTNPNRPIDVPSYAFM
                     TSAQKQLCDNIFDEWWGGRQSMLWAQYWAQNNYTDEDRYNIRQNVNDGYWRTIYTIMV
                     DLNEIIELNQNPDKKDLLSAYGANENQIASARILRAWVFQHLTDLYGDIPYFEALKAK
                     SDRVFLPPYDKQSDIYPAIIQELTEAAAQIDVTKAGWTQGDIIYKGDMTKWKKFANSL
                     KLRMALRMKKADPTNAQKYIDEAIAGGVFTSNGDVAAFKYLPSAPNTAPMYSAYYIDN
                     RTDFAVTAQFVDLLKGIPVGGAQKPNPFAGLEDPRLPIFADPVKDSTMLGPGYQGMPY
                     GMPDEITKAIGAQGSSWPGELILAPDFATILMDYAEVEFILSEINGWDQTHYINGVKA
                     SLDYWGVDAAIRDAFLAALPPASQETVLTQKYIALYTQGYEGWSEYRRTGYPKTLVHP
                     GEVTWIDLSSNPVKFEALTGDDIPRRLTYPQEEQKVNVTNYKAAVAQFGEDVFSTRMW
                     WDKP"
     gene            48156..49733
                     /locus_tag="PLF20_00465"
     CDS             48156..49733
                     /locus_tag="PLF20_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79516.1"
                     /translation="MITPSVSNDQTTSFTERDLQQMFDRFDFTDETRNIVEQENLLPL
                     GLIRRLHCAFPKYSAFREERPFMFKQKRQYIGLEGFREIVRILQSNGVKMDHLEEREL
                     FIKVYRFMATRHVLNIINWDNPSMDSLFQLVFPQPGMIRKEIVDAYFAAGTDAEKEKI
                     ISDYIRHTHPHDGKQLLNKPVFENEEGKMEVLEGCQHKYPQCELIFDVQTQNCFAFCT
                     YCFRHAQVRGDIDMFLQRDISQIHRYLRKHKEVTDILITGGDAGYLKADRFESYITPI
                     IEDPELNHIRTVRIGTRVLTYFPEMILTHDYENMLNLYRRLIDNGIQILFMSHFSTPR
                     EIMNPTTVAAIRRLRNYGVTMKSQSPIMNHISLFTDENGKVDIDRSAQNWIDLGNLLA
                     MLGVGFHSMYMARPTGEHHYFTAPLADINNIFQKVYRSLASINRPSRFITMTSSAGKI
                     SMLGTIDIEGEKAFVLKINEGRNMEWMDKIFIAKYDEKETVITRLKPFWGDKFFYEDE
                     LAEIEHQLEGKLHFHLE"
     gene            49758..50471
                     /locus_tag="PLF20_00470"
     CDS             49758..50471
                     /locus_tag="PLF20_00470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HOX79517.1"
                     /translation="MINKVVVTAAEAVADIFDGAKIMIGGFGEAGSPIELIHALIDQG
                     AKNLTVISNNTGNGHVGLAALIENLQVKKMICSFPRTAQSVVFPELYAKGMIELELVP
                     QGTLAERIRAGGAGIPAFYTPTTIGTPLAEGKEIRHIDGKSYVLEYALKADFALIKCK
                     TADRYGNLLYNKTARNFGPIMCMAAKTSIVQTSSLVEPGEIDPENVVTPGIFVKRVVK
                     ISNPLSENQLLRENRRYPW"
     gene            50465..51115
                     /locus_tag="PLF20_00475"
     CDS             50465..51115
                     /locus_tag="PLF20_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOX79518.1"
                     /translation="MVNIKGWSNDEIARKIAIDIPDGSYVNLGIGLPELVAANIPEGK
                     EIIFHTENGLLGVGPPPPPGQEDDELINAGKKPVTAIPGACFFHHADSFAMIRGGHID
                     YCVLGAFQVSESGDLANWSTGELNAIPAVGGAMDLVAGVPNIFIIMQHITKTGQPKIV
                     RQCTYPLTGRQVVKTIYTNLAVIDVTPHGLEVREIHSGMDRETLQNLTEASLSFRS"
BASE COUNT        15113 a         9964 c        11004 g        15142 t
ORIGIN      
        1 cctgttttcc gcactttttt gcaacaccct tataagtatt tgaatatcaa taattatttt
       61 ataattttgg gtgttgcaaa attttttatc atgttcattc gaaaagtaaa aaatcggtcc
      121 gggagtcaat ctgtacaagt gatacagaag gtgagaggca actacaaagt ggtcaaaacc
      181 atcggttgtg ccaccacgca gcacgaaatt gagaagctag agcaattggc gagggaggaa
      241 atggagcgtt taagcgggaa tcagctgaag ctatttgggt atgaaagtga tgagattatt
      301 gagcaagctt tttccctgct cgacaatagc aatattcgta ccgtggggcc agagttaatc
      361 tttggcaaga tctacgatta tattggtttc aatgccgttg gagaggaact attcagacac
      421 ttggtcatag cacggttggc ttttccgtta agcaagctga agacggttga ttatttgtat
      481 cgatatcaag ggaaaagcat agagatagat acggtttatc gttttttgga caaattgagt
      541 gacaaactca agcctcaagt tgaacagatt gcttttttcc atacactgca gatattggga
      601 ggcaaggtca gtgtcgtgtt ctacgacctg acgacgttgt actttgaagc cggtgatgaa
      661 gatgacttgc gcaagatcgg tttcagtaaa gatggcaagc atcaatgtcc gcaaatctat
      721 ctggggctgc tggttggatt gggaggttat gcgattggct ataatatttt tgaagggaac
      781 atcagtgaag ggcataccct gatacccttt cttgaggaaa tgagtcgcaa gttcaattta
      841 gacaaaccta tcgtggtagc cgatgcagga cttctctcca aagcaaacat caatgcattg
      901 gaagcgaatg gctatgaata tattcttgga gcaagggtta aaaatgagag tgaaagggta
      961 aaggggaaaa tactggctac ggaatttagc gatgggatga cggtgagcat gaaaaaatcg
     1021 ggcaataccc gattggtagt gaactattca gcctgcagag ccaaaaaaga tgcgtacaac
     1081 cgcaaacgag gtttagccag gcttgaaaag cagatcaaat cgggcaaatt gactaaatcg
     1141 aatatcaaca accggggata taacaaatat ctgaagttga gcggtgatat tacgatagaa
     1201 atcgattatg agaagtacga taatgacggg cgatgggatg gtatcaaagg ttatctgacc
     1261 aatacgaagt tatcggataa ggaagtaatg gaaaattacc gaaatctttg gcagattgaa
     1321 aaagtttttc gcatatccaa gacggattta cgcatacgtc ccatctatca caggatacgg
     1381 aatcggatag aatcgcatat ttgcattgct tttacagcct attgtatact gaaggagctg
     1441 gaaagagtcc tatacgaaga gaaatcggct atctctctaa agagggctac agagttgaca
     1501 caaaacatgt atcaaatcac ctatacattg cccgaatcaa aacaaacaaa atcgaaactt
     1561 ctcaacatgg atgaggagca ggccgaactg taccgaattg tttgtaaaaa tttttagggt
     1621 gttgcattgc ggaaaacagg aagaaagcgt tctctaccat cgctgcatta gtgcggatac
     1681 acctaatcag ccatttggac ttgatgtggg ttgtaaccga gggcaggcgg gcatatgcta
     1741 aacgtatttc aagccggaat aagagtccga caagcgtaca gttgtcctta ttttgataag
     1801 gggtaggttt ttaaaagaag gaaaagctaa tgtataacta actgaataag tggtatataa
     1861 aaaatgttta ggagcttttt agaattttgt cggttaatag tgatttattt tatgtttccc
     1921 ccaaaagtaa gcaaatcgaa ttgagccaat accaccatcg aaagtaaggt ttgattggat
     1981 aggaattggc ctgtttgata taattctcct tttcgattgc gaagaagcat cgagaaggtt
     2041 tttcttttgt aagtagtccg ggggattatt ccactgtaac cgatttagcc agatttcgtg
     2101 gttgatcgac attgcatcca cgaagaacgg cgatataata agccatcagt tgcaatggaa
     2161 tggttgccag tatgggaacg agcatttctt cggtttcggg tatttcgata taataatcgg
     2221 aaagctttct tactcttgta tcgccttcgg tgatgaccga gataatgcgt ccttttcgtg
     2281 atttaacttc ttcgatgttg gagatcactt tttcgtaggt acctctattt gtagcaacga
     2341 ctaccactgg catttcctgg tcgatcagtg taatggggcc gtgcttcatt tctgctgcag
     2401 gataaccttc ggcatgaata taggaaattt ctttaagttt taaggctcct tcgagggcaa
     2461 ccgggaaatt gtatcctctg ccaagataaa ggaaattacg tgcataagta aatactttcg
     2521 aaaaatctct aatcatttca gccttatcga gtatttgtcg taccttatcg ggaatagttt
     2581 ccatttcggt aagtacttcc ctaacctttt ggttggagat ggtccctttc ttttgaccca
     2641 gtgccagtgc cataagtgca agcacggtta cctgtgcggt aaaagccttg gttgaggcaa
     2701 ccccaatttc gggacccgca tgggtatatg agcctgcatc tacatagcgg gcaatcgaac
     2761 tacccacaac attgcatatt ccatagagaa atgctccttt ttgtttcgcc atttgaatgg
     2821 cggcaatggt atcggcagtt tcacccgatt gactgatggc aatcacaatg tcgtcaggat
     2881 aaatgacggg attgcgataa cgaaattctg aggaatattc aacttcgaca ggaatatggg
     2941 ctaaatcttc gagcatgtat tctcccacta agcctgcgtg ccacgaagta cctgcagcgg
     3001 tgatgattat tcgccgtgcc tccatgaatc ttttgatatt atcgataatt cccgccatgg
     3061 caatggtagt attgtcttca ctgatacgtc cccgcataca gtctttaatg gtatcaggtt
     3121 gttcgaagat ttccttgagc atgaaatgtt cgtaaccgcc cttttctaat tgatggatat
     3181 taagttccaa tttctgaata agaggatttt tttctacgtc tttgatggtt cttatcctga
     3241 gattttcgcc tcgttgtata atggctattt cttcgtcatt caggaaaacc acatcatgag
     3301 taaattcaat aatcggagaa gcatcggaag cgaggaaaaa ttcatcgttg ccaatgccaa
     3361 ttactaaagg gcttccttta cgagctgcta tcagggtatc ggggttgttc ttatccatta
     3421 ccacaatagc ccacgctcca attacatgat ttagtgctgc ctgaacagct tgtaccagat
     3481 taaagcccga agttttcttg atgtaatgaa taagttgaat gaggacttcg gtgtcggtat
     3541 tgctgcgaaa ttgatatcca tattgaatga gataatcacg aattacggta taattctcga
     3601 taattccatt gtggattaga gcaagctcgt cgttttggga aaaatgagga tgagcattga
     3661 tatcgctggg ttctccgtgt gtagcccaac gggtatgagc cataccaata gtgccatgaa
     3721 ggtctttgcc atcgattaaa ttttccaggt cggcaacctt cccctttgtc ttataaactt
     3781 gaagtttttc gttgaggata gcaatacccg cgctgtcgta tcctcgatat tcgagccgtt
     3841 tcaaaccatt aatcaatata ggctcagctt ctctgtaacc aatataacct actataccac
     3901 acatttttaa taaagatttt gttgatggac tttaatagca aatttaaggt aaaaaagtgt
     3961 aaactatttg taaacgcaat cttttttcat ataaagaagg attggtagga tttgtgcctc
     4021 cgaaaaccaa gcggtttcct ctcaccgagg ggcttgaagc aattagtgtc aaatgacgag
     4081 tactatcgcg ggttccatca aagatatcct gaatatatcg tggaatcctg aagtagtagt
     4141 tttgttgatt agaattgtat atacctccaa aatattcggt tcctatgctt tgctccgaaa
     4201 gaaaatagac ttgtcctgta gtatcttctt cgaatagaac gagattatct ggtgctgaat
     4261 agatggagtt ggcgtcggcg ttggatatga tgagttttgc ctcggttatg gctattttct
     4321 ggccatcagc cacaaggcct tttaggtcag gaattttaac tacagttttg attccgccca
     4381 tggtttgcag atagagtctt tcttttccga gggttttgtc gttatagagt acctgctgct
     4441 taaacagagg gtcagcatga gcatagccat aatggtcgaa atgattgaat cgagccgttg
     4501 ctgaattcac aacaaggctg aaggataaag agtcgctttc atcgttttta tagtacaaaa
     4561 tcaattcagt gagagcggat tcaaggttta cgtaaagtaa acttcctttg ccaggcattg
     4621 tggcatcttc tgcagtaata taaagccctt tcaggtattg gatgaactct gtattttggc
     4681 taagtgcatc ggaaggagtg gaaaatattt tttgaataaa atctggagct gattggtcga
     4741 ggcggatacg caggtgagga gcatatttca cagaatcgat atacacacta tcataaggcc
     4801 tagcaataaa actttttgag gtgagtggga cagtggaatg ttcgatcgaa ttattcgagt
     4861 aatatatgct attataacta atggtatcga ggagttcgta aacattaaca gtaagaaggg
     4921 aagtagaatc gccgtaagca cctttaatgg ctaatttaag aatagccgaa tcgatgatgg
     4981 ggttgttccc gaagctatgt ccaacaaaag gcaaggaaag ctgggtataa attcctgcag
     5041 tgctaattcc aaaaataggg tcccagtaac ttccaaggat attctggctg gtttcatcgg
     5101 tacggacaga gtcttccaca attgagtagg cgtcgagtaa aatggtcgta tctataccat
     5161 attgaagttt atcggttgga ggaattatgc tggtacctat ttgatcgggt tctttggtac
     5221 atgaaccagc caaaaaggcc aaaaaaacga accagcaggt tttttggatt atttgtttca
     5281 aagtatttgg tttttaataa acttgagggg ctctgactga ggcttgatta tttcttgcaa
     5341 aaataatcag ctattgtgat tttgaagaat tttttcgtag aaatcgttat aggcatcgat
     5401 ataggtttcc tcatcctgaa gaggaagaac gggtatattt ttttgatcga gatagctcaa
     5461 gatttcgggg tttacatttt cgcttgcaat aatagcccca tcggcaaaat cgagggctcc
     5521 ctgcataaat ccaatataag tgcccttctt gtagttggcg agatgctttt ctctgatccc
     5581 ggggagcttt attttcgatt tgatggtttt ccccatttcg ccttcaaaat catcatcata
     5641 gagagatacg atgatttttg catcggtgta gataggattg tctttatagg cagttttcac
     5701 ataaagtggg agtaaacttg taaaccatcc atggcagtgg ataatatcgg gattccagcc
     5761 aagttttttg acagtttcca gcactcctct tgcaaagaat accgctcttt cgtcgttatc
     5821 cttgaaaaat ttgtcgttct tatcgtaaaa ctgaaacttt cgggcaaaat agtcatcatt
     5881 atcgataaaa taaacctgca tgcgtgcttg ctgaatagaa gccaccttga taatcagagg
     5941 ccgatcggta ttatcgatga taatattaat gcccgataat ctgattactt catgaagctg
     6001 attacgcctc tcattgatat gcccatatct tggcatgaat gttctgattt cttttccctt
     6061 ttcctgaata ccctggggca gatatcttga aatttgtccc ataggactgt cctttaaata
     6121 aggaaccatt tcttgcgaaa caaagagcac acgagcttta gtcattatga aaaaattata
     6181 aaaattagtc gtcttttctt tgcaaagtta cgattttttc cttaaataga gaaaaaatcg
     6241 attgattact aattctatat aaaatttttt tcgaaacacc tcactgttag caatacaaaa
     6301 cccctcatga atcatgtgtg caggaatagt tctcgagatg caaaggattt cccacatttg
     6361 actctgcatc atactatatc gaggggggct tgtatttttt catgtttggc gataattctt
     6421 gattttctga ttcgattcga cagtgaaaat ttcatgaatt taagacttct ttcaagatct
     6481 tgtgtgtatg aatttcccca aagccaggta aatgctgatg gtagtatcga atgagaatgt
     6541 ctatgatggc tgttttatct ttgatgtgaa tattgggtat aaagtagtcg gatgagctaa
     6601 aggctttaat gagaagagaa agcgttttga cattttcacc cgccacataa gtatttcgtt
     6661 ctgggacatt ttcctgaaac tttccctctt ccatattgaa ggttatagtt tcggaggagt
     6721 agttatccag aggagtaatc cccagaaaac tcgaaagcct gatgagaaac cagatagggt
     6781 agaatgcaac attttcttcc tgggtttcta acctaatgat ggttttttcg agaaaatcat
     6841 acagcaagat gttggcttct tcttctttga tgctgtggtt tagaatttcg gccatgaaga
     6901 gtgtaatacc cgccttgatc acatgatttt gtaccttgta acttaaaaag taattcctcg
     6961 cttctttcag gaagaaaata cgattgtgcg gtttttcttc gtatacaatt tcaaggaggc
     7021 tcagaggcaa aagctgtgaa ggtttcagtg aagggttctt cttgttgatg cctttgatca
     7081 tgaatgcttt tatacctccc tggcgagtat aaagcctggc aataatgcta tgatcggtat
     7141 aatgaatgta gtggagtaat attgccggtg tgcgaataat attaggaatg atgcagcaga
     7201 tttgaggtta tgattcaaaa ttacctcttt tcgactgttt ttgatcatga taggcaggcc
     7261 gaattttctg ttgtaggatt caaatagcaa accttgggca atttaaggga taacagatgg
     7321 ttttttccac cacctattca taaatgccag catttacttc attccttaat accatgaggc
     7381 cgtttatggc cagggcactc atttcatctt ctccgggata aaccacaatg ggagcgatga
     7441 agccaatcat ttccttgatg taatttacca cataggtatt atgagctatt cctcctgtaa
     7501 gaataatagc atcgacattt cccttcaaca cgcttgccat agctccgatt tccttagcta
     7561 cctgataggc tagggcgtcc tgaatgaggg cagccttctg attcccctca cgtgcggcta
     7621 tttccacctc ataagcattg ttagtattca aataggccat atatccccct ttgcccgtaa
     7681 tcatcttttt tacttcctcg agggtatatt ctccactgaa acataaggct gccaactgtc
     7741 ctacaggcaa agttccgcta cgttcgggcg aaaaaggacc ttcaccatcc aaagcattgt
     7801 ttacgtcaat cacctttccc ttttgatgag cgcctaccga tataccgcct cccaagtgtg
     7861 caatcaatag gttgaggtct tcgtagtttt tgttctgcga tcgggcatat aatcgagcta
     7921 cggctttctg gttcaaggca tgaaagatgg aaactctctt gaagagaggg tggccggcaa
     7981 tgcgtgctac gtcctgtaat tcgtctacga ctaccggatc ggctatgtat gctttagcat
     8041 ccttcaacga gcgagcaatt tcgtcggcta taatgcctcc caaattactg gcgtgttcgc
     8101 ccatcaggcc agctttcaaa tcttcgatca tacgagagtt cacttcatat attccgcttg
     8161 gaataggttt gaccaaacct cctcggccaa ccaccactct tatgctgttc aaatcgattc
     8221 cggctgcttt tagttcttta agaatgatat ctttcctgaa tgaaaactgg tcggtaattt
     8281 ttcgataaca ttgcaattct tcgggagtat ggcgtatggt tttcaggaag atattcttgt
     8341 ttccttcata tacggcgatt ttggttgagg ttgagccagg attgatggca agaatacgtt
     8401 cattattcat aggataaatt gttattattt gccatgaggc aggcaagtgc taaacagtaa
     8461 aatttcgaat tttcgctttc gcttctcgac atcagaacta cgggcttgcg tgttccttgg
     8521 agcaagccag ccaattctgc gccagcaaac cttacaaggc ctttatagaa tgcattgcaa
     8581 ctttcgagag aaggaaagat caaaatatcg gcttctcctc caataggagt gatgattcgt
     8641 ttaatgttca ggctttcggg ctgagtagca agaaaaatat cgagtgggcc atccacaatc
     8701 acttccccaa gttgtccgcg ttgagccatc ttgcatataa tactggcatc tatggctgtt
     8761 tgtgattgat ttccgacttt ttcggaggct ccaatcaaag caactttggg tttggaaatg
     8821 ccaaaccttc ttgccatctc caccgcgtat ttcaacatgg caattttctg ttcgagattg
     8881 ggataaggga tgactgccgt atcggaaatg aaaagtagtt tgtggtaaga agggatttgc
     8941 agagcagcta cataagtcat tacttcgccc tgtttcatca gtcccgatgt tttatccagc
     9001 acggctttca gaaaaatatc cgtaccaatg agtcctttca tcaagatatc ggcttttcct
     9061 tgatgaatca gacttaatgc ctctttcatt gccatttgtt catcgggtag atgaacgatc
     9121 aaaaaagttt cgggaggaag ttccaggtgg gcaagcaatg attctgttcg ggtcttgttg
     9181 ccgataagga tagccttggc aaatcccgtt cttatggccc gaacgatggc tcccatggtt
     9241 gcatgatctt cggcggccac cacagcaatg gtttgagggg ctgctgtatt taatttatcc
     9301 accaattgcg taaaattcgt gatcatagcc gggaaatgac gaaatatggg tcattaatcg
     9361 gttgacagag cagccagggc aatggaatag gttttagtaa gggcgctatc gcctcttgag
     9421 gagaggactg ccgggcattt ggctcctatg acgatggctc ccagttcgcc tttggcgagt
     9481 tttgtattga atttgtagaa aacattacct gtttcgatat tagggaaaag cagacaatcg
     9541 gcgtcgccgg ccacttcgct cttcagcttt ttaatggcag cgctttcttt gtcgatagcc
     9601 gtgtcgagtg caagaggacc ctctataagc gctcctttga tttgtccgcg ttcacccatc
     9661 tttgaaatta tggctgcatc cacgcatgtt ggcatgttcg gtaacatttg ttctgttgcg
     9721 gcaataattg ccaccttagg cttttcatta cctaaacgat gagcagtttg tatcaggtaa
     9781 ttcgtgatgg caatcttctg gtcgaggtcg ggaacaggta taatggctac gtcaccaaca
     9841 attaacagtt tttcgtaatt cgggttttcg ataacggtta catgactcaa gattgccttt
     9901 ggtggcaaca aacctgcgtc tttattgaga atagccctca tatatttatc ggtggtcaca
     9961 aggcctttca tcagaatatc gccttctccc tttacaatca attcaacggc tttgtgagct
    10021 gccttttgct catccgattc ctgtacaatg cgaaatttct cgggttggat attcaaatcc
    10081 ttgcaaacct ttacaatagt ggcttcatcg ccaacaagcg tagcatctac aattcctaat
    10141 tccatggcat gatgtacggc ttcgatagag tggttgtcgt tggcgcaagc cacaacgatt
    10201 ctcttacgag ccttagcctt tgcagcttct atcatttgtt ccagatgagt aatcattgtc
    10261 gtataagatt aagtatattg ttcattgagt ataaaaatcg agcatttttc gaatagctcg
    10321 tatgcaaacc gggcaaaagg tgttttgacg aaaagtattc atcaggcagt ccatggctgg
    10381 gcgataaatg cctttagcag catagcctcc tccttcgaaa acacccacct tatttctata
    10441 aatttcttcg gcaggggtag ggatgggagt tccttcttct atcatatcgg cccattttct
    10501 tttgaaattt accaatgtag taatattggg ttcctgcggc tcgaggtcga gaggataaaa
    10561 ttcattgtaa gccacctccg aatcatagta ttcatcggct aatccggcaa aactatgccc
    10621 aaattcatgg atgaagacct ttccagcaag catgttgcta ttcaccgaaa gagcataata
    10681 attgaagatt gcaccaccac cgtacatgga tgagttgact aaaatataaa tctggtcgta
    10741 aggagcattg gaagcaacat ctcttactgt ttgaaatgca tcggtcatac agtaacgatc
    10801 gagatcgaag gtatagaatt tcgaatgaag caaggtattc ttccagattc ccgatggtgg
    10861 aaaatcgctg ctttcttcaa atgaaggagc taaaacggct ttgatattga ttttatcgct
    10921 gtattcgtta taaggagaat atcctaagag tatttccgca aatttttgac aatcctgctt
    10981 gaagatttcc atctgctgtg aagtatatcc ctcgggcaaa atcacaatat cgagggcttt
    11041 ttctgccgga gcaacttctc tgatcttgaa gacagggaaa accattcttt gcttttggtt
    11101 cacaaaatag tcgttgacgc gaaatggata ggaaaatttc ttttcgaatt gtcccttcct
    11161 gttccgactg tagatttcga tgaaggcatc atgacgtggg aaaggcatga caacggattc
    11221 ttcgaatgct ctgtaaacat tttgtgcttc aagtgtagtt tgccattccg agaacaaagt
    11281 ggaataacct ctcgaataaa ttaactcacc cgtagcctga tcgtagagcc gacactggaa
    11341 tttaccataa ccaaaggtat cgatcaggtg aaggtgtgaa cctgcccaat aaggttcttc
    11401 gaggagttca tccaatgcaa aaaattcatt cctggcgtct ccaatatgta ggtaatcgaa
    11461 gcgtagggtc ttattgagga agagagagtc gaattgagca tgaatgacgt ttgggtgtac
    11521 caaccaactc aaaacgaaca atatactcag aagatgttga tgaaaaaagt gagccattat
    11581 ggagaaaatt taataaatta aatcacaatg ttttatgttt gtgtttttcg ttattagcct
    11641 gcaagcaaag gtaaagagct tttgttgatt ttcggcaaat cgatgaaaga gaaaaggaaa
    11701 ataaaccagt tttggttttg agattttttt ccgatttttg cattgtaaaa tacgaatctt
    11761 ttaattttac gtcgataatt ttgaatatgt ggtttgaact gctttcttca ttagcactat
    11821 gcagatattg tcgtatcaag tgccgcgaca attattccat ctgaattccc cagccgggtg
    11881 ttttttcttt ttccatattt tctatagaga tttttccagt cgaaagatta aacaaaacca
    11941 ttatcataat taaaaacttc aattttatgg aattaccatt tgctgaatat taccggataa
    12001 agatggtaga gccgatttat ccgagcacgc gtgaacagag agaggactgg ataagacagg
    12061 ccagatacaa tctgttcaat ttaaaaagcg agcaggttta cattgatttg attaccgatt
    12121 ccggcactgg cgctatgagc gaccgtcagt ggagcgagct gatgcttggc gacgaaagct
    12181 atgcgggagc cagttcttat tacaagttaa aacagtccat aggtgaaatc tttggtttcc
    12241 catactttct ccccactcat cagggacgag cagccgagaa tgttttattt tcggctttgg
    12301 ttaaggctgg agatgtaatt ccaggcaatt cacctttcga tactaccaaa ggacatattg
    12361 aatttcgaaa tgccacagcc atagattgta gcattaatga agcttttgac accagtattt
    12421 ctcatccttt caaagggaat gtcgatttgc agaaactcga ggatgtactg aaggcgaatc
    12481 ccagagaaaa aattcctttt gtcttaatca ctgtgacctg caatacttcg ggtggacagc
    12541 ccgtttcgat gcagaacctg cgcaaagtga aagccctggc agctaagtat ggtattccgg
    12601 tttgctatga ttcggctcgt tttgccgaga atgcctactt catcaaaaaa cgcgagcctg
    12661 aatatcaaga taaatcgatt cgtgaaatcg tgaccgaaat gtattcttat gccgatttgg
    12721 ctaccatgag tgggaaaaag gacggaatag tcaatattgg ggggttcata ggatttcgtt
    12781 cggaggagat ttttcggcag gctaccacct tcaatatcat gttcgaagga tacattacct
    12841 atggcggcat gtcgggtcga gatatgaatg ctatggcaga aggcctgcgc gagagtacta
    12901 ctttcgaata tttagagaca cgcataagac aagtggaata tcttggaaaa cgactcaccg
    12961 agtttggaat tcccattcaa caacctgttg ggggacatgc agtttttgtg gatgccctga
    13021 agtttctgcc taaagttcca cgtaaggaat atgtggctca aaccttagct gtggagctct
    13081 atcttgaggc tggaattcgt gccgtagaaa taggaacttt attggccgac agagatcccg
    13141 ttacacgtga ggatcgttat ccggctctcg aaacggttcg actggccatt cctagaaggg
    13201 tttacaccaa taatcatatt gacgttgtgg ccgttgctat caaaaatatc tgggaccgcc
    13261 gcatggatat ccagcatggg tatcgaatta catgggaagc tcctatcatg cgccatttta
    13321 ctgttgagct cgaaaagtcc gataattgat cccgacattc tgcctcgaag tattctcaga
    13381 aatggaattt taagttcatg agccaacata ctggcacgat gtgtccttgg tttctttttc
    13441 tttcttaagc atccttggtg aatttcaaca tagtaggtaa aaaatcattt tcgtaggctt
    13501 tttcggaatt tggttagggg aaagcaattg tgtcgtagct attcattttc agggtattat
    13561 aaacgatagg aggtttttct attatgcacc ttcatcgttt ctttggtgtt tttatcgata
    13621 ggacgccaat gtcagccata ttcggcgtca gacgtggtgg ggagctgaca tgaggtcgat
    13681 ttctggcaaa gagtatctct cccttttcga acctttccat tttgacatgg tttttccgaa
    13741 gcaaaccttt cgaaatattg ttttgtcaag aaattctgac tggatgaagg ccgatggttt
    13801 gaatgtagca aagtttatca atggtttggg ttgttatttg aaatgattat gaataggaga
    13861 ttttccgttt ttatggggtg tggttcagaa ataaacccag taattttgct gtgaaatttt
    13921 tcacaatgtt tttttattca cattaaagga aatatcatgt ataaaattac cacacatccc
    13981 attcttgagg ttcccaaggc agaaaaggtt acttttagat acgatgggca catcatcgaa
    14041 gctgagaagg gttataccat agctgcagcg cttcatcagg ctggatttcc tgtacacagt
    14101 cacagtttac gcaatcgtaa gaggagtttg gaatgtggca tagggaaatg cggtgcttgt
    14161 gaaatgctgg tgaatgggca ggtaaagcgc atttgcatta ccaaggtcga tggagtgcat
    14221 gaggtaaaag aaatccctca tgattacagg ccgggggttg tcgaatatcg caagaatgaa
    14281 cccatcgatg tttataaaac ccaggtcgca attattggtg ccgggccggc aggtttggct
    14341 gcccgtgaaa tcttatgtca gtttgggatc gataatttgg tagttgataa caacagtaag
    14401 attggtgggc aatttctgat gcaaacccat cagtttttct tttttgagaa ggaaaagaaa
    14461 tttggaggga tgcgaggttt cgatattgct caaaccctag ctgggacaga ccatgatggt
    14521 atatttttga attcgacggt atgggatatt ctggaaggag gtcggcttgc cgtgaaggaa
    14581 attacgaccg acagaatcta ttatatcgat gcagaatatt tggttgtatc taccggggct
    14641 gttccattta tgccttcctt tgagaatgat gacttacccg gagtttacac ggctgcggta
    14701 gttcaaaaga tgatgaatat gcagtttacc ctcttgggta aaaatgtttt gacggttgga
    14761 gcggggaata ttggatattt gacttcttat cagcttatgc aggcgggagc aagggtaaaa
    14821 gtcattttgg aggctcaacc ctttgaaggc ggatttcccg ttcaggcgaa tagagtgcga
    14881 agattgggaa ttcctgtact gacctcgcat attctgctca aagctatacc caatcgagat
    14941 ttgacgggaa tcacgggtgc tgtaatagca ctttgcaaaa attttcatcc tgttgttgga
    15001 actgagactg tagtcgaagg tatcgatgcc atcaatattt gtacgggatt ggtttcggat
    15061 gatcagctac tgatcaaggg gaacgaggtt tttggacgaa aatgctatgg tgctggcgat
    15121 accattcgca ttggagaagg cacgagtgcg gttctgagag ggaagcaagt agcctatgaa
    15181 attcttatgg aaatgggaat tcgtttgaat tatgacgatt atctttcact ttcgaaagaa
    15241 tatatcgatt cgcagcaaca tccaataaga atcatcgatc gaccttacat gcccgaccat
    15301 gaaaggatgc aagccaaagg attcgtgttg atcgattgtc tttatggatt cgcctgtaat
    15361 ccttgtgctt ttgcctgtcc tcagggtgcc attacaaaat cttcgaccag tacagttcct
    15421 tccatcgatt tcgacaagtg cattggctgt atggattgtg tttatcaatg tccgggctta
    15481 gctatttttg gttataattt gaaacgcgat tgggtatttt tgcccataga atatgatgta
    15541 aaggaaaatg ccgaagtttt tctcgttgac aattatggaa aaattttagg agaaggggta
    15601 atcgataaga ttcttcggaa acccaataaa accaatattg ctcgagtaaa gtcgctcagc
    15661 ctgcatggag aagatttaac acaagtgcgt agcttcatag tcaagaagaa ttatccagtg
    15721 cctttagagc tacggcctta tgatgaacag attccggttg aaagttatat ctgtcattgc
    15781 gatgatgtaa aaattgagga gattttagaa gtggtaggtg agaggaaatt catatccatt
    15841 gatgagataa agcacactac acgtttaggg atgggtgcat gtcggggaaa acgttgcata
    15901 ccccgattga agtccaagat aaaggattat ggaattcaga ttgtaggcga agccacgccc
    15961 cgcggaccga tgtcgaatca actttcgctt ggagagcttt atcccaggcc agttcatgaa
    16021 aatttcgtag taaaccgttc tgtaagaaag gagaaagtgg aaactctaat agcaggtgga
    16081 ggcatgggag gaagtgcctt atttcgatat ttatccgaag caggcaagaa gccggtattg
    16141 attaattttg gccgtggttc ttcatggagg aatattgcag gaggccgtcc caatttcagt
    16201 gttcctgagc tttccgatat tgccgagcat aaccgccaga tatttatcga attgcaacaa
    16261 ctgcacaaca tcgactatca tcccatacaa tacgtgaatt ttgcacacga tgaagaaatg
    16321 tacaaggcac tggaagctgc catggcatgg agcaaagccg aaatgatcag tccgaaggat
    16381 tttacgaaac ggatttctcc atttttcaac ccaggtttaa aaacctatat tgctgcacag
    16441 attacttata attgttggca ggctactccg ggcaaaacca tcgatttaat ccgtcggatt
    16501 ggaattcagc aaggaggtac gattttggaa gattgcaagt taatcgatgt aagaaagaat
    16561 ggtcaggaat atcaagtttt ggttcaagat cataccaaac aatacattga atacagtagt
    16621 gaaatcttca tcaatgcatt agggccagaa gccgcatact ttgccagatt attaggaata
    16681 gaaacgggtc tctatcccgt acgccatcag gcatttatta cgcgtcgaat gcctatgctt
    16741 ggtgtcaacg atataccact tccgatgttg atcgacagaa ggaaatacaa gggtttcgtg
    16801 gccgtttatg ggcagcagct agccgaaacg ggtcaggtca taggctgtgc ttctccggca
    16861 gtagatcccg ccgaaaccct caagaatttg aaagttaact cgagagaatt tctcgaaatt
    16921 ttctcagaag ttttcgtgga ttggttgccc gaattatcct cggtgggttt tcaggcggtc
    16981 tggtcaggtt attatgtcga accacgaatg atcatagacc cggatttagg attatttgtc
    17041 gggctccgcg gccaaggttt catgctaagt cagtaccttg ccaagatgta tgtcgataaa
    17101 ctcacaggca ggcctgtacc cgaatacttt tcacgtttga gcattcaggg cgatggattg
    17161 cccgaaaaag ccttcaaata aatattcggg ttcatcgctt tatttagggt tttcattttc
    17221 gaacatttta tttcgtaatt ttgccctcca atttctgtga gaagtgttga atgcattgaa
    17281 accatatcat ctgggattct caggcttggc gctgggtcat catcattacg atttcgacat
    17341 tggatcgaag tttttcgatt gtttcgaaga aagcgagatt catgctggaa aagttaaagt
    17401 ggatttggat ctggaaaaac agaaaaagat gttagtgctt catttcgata tccatggtgc
    17461 ggtggaggtg gtttgcgatc gttgtgccta cgaatatatg caacctatcg cagggaagga
    17521 agaggtttac ataaagttcg gccctgaata tatcgaagaa gatgatgacg ttttggtttt
    17581 accccaggga agccatgaag tggatatttc gcagatgttg tatgatttta ttcatttgat
    17641 ggtaccatat catcgtttgc acttacccga tgagaatggg aatccggaat gcaacaagga
    17701 gaatcttgaa attctggaaa gacttgcacc aaaggaaaat caaatagatc ccagatggga
    17761 agttttaaat cgtttaaaaa ccgattgata tttcataatt caaactaaaa attgtaagag
    17821 atgccgaatc ccaaaagaag atggtcgaag accagaacgc gtaagagaag aacccattac
    17881 aaagccaaga tgccggctgc ctttgcatgt cccaactgtg gctcaccagt gctttaccac
    17941 agagtatgtc cttcgtgtgg tttctataga ggccgtcagg taatggaagt cgaaaagatg
    18001 aaataaattt cttcatactt aaaatttgta ttgattgaaa gattctttga caagagattt
    18061 ttgtgagaat tttttttact tattgcctga ggttaaatcg tttcgaagca gaatacatta
    18121 caatctattt taattgcaat taaaatgacc aagatacgtg ctgcaattac gggagtaggt
    18181 gcttatgtac cggattatat cctgaccaat gaggagatta gcagaatggt tgatacctcg
    18241 gatgaatgga tcatgacgcg ggttggaatc aagactcgaa ggattttgag agggaaaggc
    18301 aaaggtacat ccgatttggg aactcctgcg gtgaatgaac ttttacgcaa aacaggaaca
    18361 aagcccgaag agattgattt attgatttgt tcgacggtaa cccctgatca tctttttcct
    18421 tctacttcgg caatcatcgc cgataaggtt aggattaaga atgccttttg ttttgatttg
    18481 aacgcagcct gttcgagttt tcttattggt ctttcgagtg ctgcgaagtt tgtagaatcg
    18541 gggcgatata agaaggtagt gttggtaggt gccgataaga tgtcatccat tacggattat
    18601 accgatcgta gcacttgccc tttgtttgga gacgctgctg gtgcggtgct tatcgaaccc
    18661 accgctgata ccgaagttgg ctatatcgac gaaatacttc ataccgatgg atatggccgc
    18721 atctatctgt atcaaaaagc cggaggttcg gtatatcctc ctacccatcg tacggttgaa
    18781 gcccgtgaac attacatcta tcaggaaggg caagtcgtat ttaaggccgc agtagcctct
    18841 atggccgacg ttgccgtcga aatgatggaa aaacatggat taacttctga aactattcat
    18901 tggttggttc cccatcaggc caataaccgt atcatcgaag cagtagcccg acgtatgcgc
    18961 ctgccgaaaa aaaaggtgat gataaatatc gataaatatg gtaataccac ttcggccaca
    19021 cttcctcttt gtttgtacga atgggaacat cagatgcata aaggagataa tatcattctg
    19081 gctacttttg gtgctggctt cacctggggc gctatctatc tgaaatgggc ctacgatccc
    19141 agataaacca aggtaatcat aattgccttt catgaggaga atatttctgt ttattgcgtg
    19201 tgagttgatt ttcttttagt aattttgttt agataacctc ctcagaaatc ctcccgaaac
    19261 tatgaaaaaa ctctatctgg catttatctc ttgtttgttt atcgtcgttt tgaatagttg
    19321 ttcgaaagac gacacttcca tcaatatttt taccatcgag gatgatatag ccttaggtga
    19381 gcaggtttcc aatgagatag ccgcaaaccc tcaggaatat cccattctcg atcctgtcaa
    19441 gtatgcttcg gcttatgatc atctttatcg gattcgcaat acgattcttg cttcaggaaa
    19501 ggtaaaatat gccacaaagt ttcgatggaa ctgcaagatc atcaggaacg atactatgat
    19561 taatgcgttt agcctgcctg gaggcgacat gtatttctat acgggtatca tcaagttttt
    19621 ggataatgag gcgcagtttg cgggagtaat ggctcatgaa atggcccatg ccgacaggcg
    19681 tcattctact gatcagctca caaaagtcta tggtttggag attttattag gagttttact
    19741 tggtagcaac ccaaactttg ccgaacaaat tgctgctgag ttagctttag gccttggcac
    19801 gcttgccttt agccgttctc aggaatacga agccgacgaa tatgcggtaa agtttttata
    19861 tgccactgaa tatgatgcgg ccagtttggg agatttcttc gaaaaaatgg aaggcgagcc
    19921 atatccacct acattcttaa gtacacatcc ttctccagaa gacaggctgg cgaagatcga
    19981 agagcatttt caggctctgg gtggaatgca tggcgccact ttcgcagaac gttacaagga
    20041 atttaaaaat gctctccctt aaaatttaat tatagcactt gaacccttta tggtttattt
    20101 cttcgaaacc tttcgagagg agaagatcga gggtttgcga aaataataat ccaatctcgg
    20161 atggatgatg agcatcggcc gatataacca cagggatgcc taattctttt atgacaggaa
    20221 gaaatgtttc ggacgggaat agttgatcgg acagtttctt atagagtccg cgcgtattta
    20281 tttcgaggat agccccgcat tgttggacct tcatgagagt ttcgatgaca agataacgat
    20341 accagggttc attttccgag aaataacgat gagcattata catcattatt ttatcgagat
    20401 gacctacaat atcggttttt tctcgttcta tcatttcctg tatttgtcga tagtaaagat
    20461 tcacacccca tttaacatcg ccttccatta cttcctcgag acccttgtcc catatttctc
    20521 ttttggggcc atcgatgaac catagctctt cctttgcaac atttctcacg aaatgaaccg
    20581 aacctatggt ataatcaaga tggcattccc tggctaattc ttcgaagggt ttggaaactc
    20641 cggggatata atccatttcc aaacctaata aaatactcag tttattttga tacttcgatt
    20701 tgagtgaatt tatttcttca cagtaagata taagtttttc gttgggtata gcaaaattat
    20761 tggcgaatgg cacaggtgca tgtcccgaaa aacctaaaga atagaaatga agctgaaggg
    20821 cattttttac atagagttca ggagcggaag ttccatcgca gaaataggta tgggtatgat
    20881 aacaatacat cgacaaaggc tttaaggttc tatccaatcg ccgttctgac ggattaaatc
    20941 gatcaattca tccacggcca tttcttctgg aatatttctt ttcacaggag catgtccttt
    21001 ataaagagta atttttcctc tacctgcacc tacatagcca taatcggctc cggccatttc
    21061 accaggtcca tttactatgc aacccataac agcaatagta agccctttga gatgagaagt
    21121 gcgctccttg atgcttcgaa gtgtgggttg aatattaaaa agtgttcggc cacacgaagg
    21181 gcatgcaatg tattcggtac gggagatgcg tgctttaacc gattgaagga tggaaaagga
    21241 aatgttgacc agctgttctg gctttaattt gggattttcg agccagatgg cttgtcccaa
    21301 tccttcctgg aatgcaggtc ccattaccgc agcggattcg gtaatgaatt tctctggttt
    21361 ggcctcgtaa gagcttcttt ttataataat aggctgattc ataccctgat tcctcagatg
    21421 tataactgct gccctcaggg caatcaattc acgaggatgg tcggaatgaa tcactatggg
    21481 caattcttcc tgaagtcttg caatctcaag gtgagatgga aacgattggg tatgaatctg
    21541 atgggttcga aatgggaggt cggataaaat aacgatatct gcagatgatg tctgaccaat
    21601 cactaaagga ggattcatac cgccgatttc atgtatttca ttgctaacga atttttcttt
    21661 ctgaaagttc tcataacgat agttttttaa accgaatgca agaggattta acggaaaaac
    21721 actggtcatt tctttgagta tagaggctac tggaatctcg gcttcaggtt cttcggtcaa
    21781 tgacacgcga atagtatcgc caatcccttc ggcaagtaaa gctcctatgc ctgcagccga
    21841 ttttatcctt ccatcttctc catcacctgc ttcggttaca cccagatgta caggataaac
    21901 tttgcctctc ttcaacatcg tcattgcaag taaacgattg gcttcaatca tgatgcgagg
    21961 gttgctcgat ttcatcgata aaactatatg atcgaaatcg aggtcttcgc aaatctcaat
    22021 aaactcgatg gctgaggcta ccattccttc gggtgtgtct ccataacgag aaaggatgcg
    22081 cttcgacagc gatccgtgat tgcttccaat acgcaatgca gttccatatt gacggcatat
    22141 ttccaataaa ggttttatat gatcgtatat gatttctaat tcctcccgat attccttatc
    22201 ggtataagta atttttccca tgtgcgggcc aatgtaattg cctggattga ttctcacttt
    22261 ttcgacaaca gcagcggcaa tttcggcggc tttcggactg taatgaatgt cggctattaa
    22321 gggctttgta tatcctctta ggtggagctc ttcttttatc gcttttagat tcgaagcttc
    22381 tttaggtccc tgggccgtga tacgcacata atcacaacct gcatcgaaca atcgaatggc
    22441 ctgagcgaca gtggcttcgg tatccatcgt aaaagtattg gtcatgctct gaatacgaat
    22501 gggatttgtt cctccaaccg gtatatcccc aacgggaatt tcaatggtcg ttaatctttg
    22561 ataatctttt tgaatataga atggatattt ttccacagat atgcattttc tgcaaaatta
    22621 aaatcattcc tgtctaatat aaaagattcg acataaaacc ttcctgacga cttggaaaga
    22681 aataaattcg aattgcagcc ttattcacat tacccattat gtttggctag attgctcact
    22741 aacatgtcat tcttcgttca gtggtcgatg attgataaaa attctttttc ttcttttatg
    22801 acctttttat ctttagtttt gcctctctaa aattttctat attcttttct gtttatcgat
    22861 aaatatttta ggtttatgcc aagacccaac gcttccgaac ttgttctcag tgctgatggg
    22921 aatatttatc atttaaggat aaagcccgag cagcttgccg atacggtaat tttggttgga
    22981 gatcctggtc gggtatcaat ggtttcaagc catttcgacc gtttggaatt tcaaggacag
    23041 aatcgcgaga tattggttca tacgggttgg ataggcaaaa caggaatatc ggttatttcg
    23101 accgggatgg ggccagataa catcgatatt gtagtaaatg agttagatgc gttggtaaat
    23161 atcgatctaa ctactttcga agaaaaaccc cagaaaactt ttttacgact tatcaggatt
    23221 ggaacttcgg gaagccttca gcccgatatt ccagtcgatg ctttcgtagc ggcggaatat
    23281 gccataggcc ttgatacttt attgcgtttt tatcgtcccg aaggaaattc attcgatgaa
    23341 gaaatagccg aagaatttat ccgtcaaaca caatggaagc tcgacatggg taagccttat
    23401 gtggttaggg gttcttccga attattgagc ttgatgggtg aagaggttgt aagaggaatt
    23461 actgcgactg caccaggctt ttatggcccg caaggtcgta aattgcgttt accactggct
    23521 atgcccgata tggacgaacg attgatgagg tttcgctatc gtgatcgttg tattaccaat
    23581 ttcgaaatgg agacctcggc tctgtatggt ttatcggcac ttatgggaca ccaggctctg
    23641 actatttgcc ttaccattgc caacagagct acaggaaaat ttagccctga ttatcgttct
    23701 cgcatgaacg aacttattcg tttggttctc gaaaaaatga ctcattcata gaattttatt
    23761 catgaatcta tatttgaata gtcctccccc attttgccag cccgaagttt tgctcgatat
    23821 tcctaaatgt aaggccaata tcgaaaagat ggtgaacatc agcaggcagg aagggattat
    23881 atttcgtcca catttcaaga cgcatcaaag taagggaata gggcgatttt tcaggcaggc
    23941 aggagtaagt gccattacag tttcttcggt tagcatggcc aaatattttg ccgaagatgg
    24001 ctggaacgac atcaccatcg cattccctgt aaatctccgc gaatgggaac agattaagag
    24061 ccttgcctcg atagtgcatt tatcactact tatttcagac gagacatctt tacagaaaat
    24121 ctcaagactt gattttttga gctttcattt gaattttttc atcgaattag acacagggca
    24181 ggggagatct ggatttgaat actcggctga gaaaatttta cctcttattc gattggccga
    24241 tgaactccaa atacccatcg aaggtttcct gtttcacgat gggcatactt ataaggctca
    24301 taacactgat gagattagaa aaattcgcgc ggccaccctt gctatgacag ctcatttacg
    24361 tcatgatctc gaagtattct acccaggaag aaaatttttg tattcgatgg gagatacacc
    24421 ttcatgcagt attgacaatc gttatgaagg catagacgaa atgaggccgg gtaatttcgt
    24481 cttctacgat ttgatgcagt atgatttggg tacctgtacg caggatgaga tcgctattcg
    24541 cttggtttgt cctattgtag ccgtatattc tgaacaggaa agagccgtga tttatggcgg
    24601 agccatacat ttagggaagg attttttaac tttacctcac aacgagatat gttttggggc
    24661 tatttgcgct catggagatt ctattgaatg gcaagggcga tatccgatgg cttggctaag
    24721 ttcgctctcc caagaacacg gcgttgtaaa ggctccaaaa gattttctca aaaagattca
    24781 gcccggcgat cttctcgatg tcgttcctgt gcattcttgc ctgatggtaa gtgcagccct
    24841 gagaataata ggaagcgacg gtacaatttt tgatactttt aacgcaaact tcatgaaaga
    24901 tacataattt caatttattg agccaacagg aacttcttct ctatacttat ggaaaaaaag
    24961 gaaactataa aagaattgat agcattgata agtatggtag atgaacctga tgaagtagtg
    25021 tatggaagga ttcgtcagcg cattatcgac ctgggagaag aagccttgcc atcactcgaa
    25081 aaagcttggg aaaattcctt cgaagaattt actcgtttac gtatccaaga attgcttgat
    25141 acgattcatt ttgagagtat tcagcagcag ttgattactt gggtaaattc gaatcaggat
    25201 gatcttctcg aaggagcaat acttctttct cgttatcatt tccatgaact cgatatcaac
    25261 agaattacac acaatctggg acagatcatt caggatgtat ggctcgaaat gaacgatagg
    25321 atgaacccgc tcgaaaaaat caaggtgctc aatcatattt tgttcgatgt tcataaattt
    25381 acacctacca agcctcctca atattccctt caagattatc tcattcccaa tttaatcgat
    25441 tcgaaacgtg gaagtggtac tactttgggc attctatacc ttatcattgc ccaaagcctc
    25501 aaattgcccg tttatggggt gaatttgccc tataatatga taattgccta tcttgagaat
    25561 aatccgggca tttatattta tgaccctgaa aacgaccccg tgctttttta tatcaatccg
    25621 gtattcaagg gaatgatatt cattcgcaag gaaattgacc gttttttgaa agataataag
    25681 attgaacctc aacctcaata ttttacggct tgcaacaacg tacagattct tcaacgtttg
    25741 gcaagcgaaa tgcgcatgat gtacgatatg gagggtgaca gtgaaaaagc cgaaagaatg
    25801 gaccagatta ttgccatttt gaagtcataa acggggaaga tatttttcga tcttaggcaa
    25861 ttggcttaat tatttgcatt ttataagcct ccatttttat tatgaaatcc tttccgtaaa
    25921 tttgttttcg aaacttaaca agaggatgcc tgaacagaaa ttgtttctac tcgatgccat
    25981 ggctttgatt taccgggcct tttatgctat ggtgaaaaca cctcgtatca acagcaaagg
    26041 aatgaatacc ggtgctgtct tgggatttgc caatacacta tgggatgtac taaaaaatga
    26101 aaatcctact catattggtg tggctttcga catgcaagcc cctactattc gtcacgaaga
    26161 ttttgccgat tataaagcca atcgtcaagc aatgcccgaa gatcttgccg cttcgatacc
    26221 ttggattaag gaactcctta gggctttcaa cattcccata ctcgaacaag aaggatacga
    26281 agccgatgat attataggta ccttggcagt gcaggcagca gaaaaaggct tcaaggttta
    26341 tctgatgagt accgataaag atttaggcca gttggtaaat gaaaatatac tgctctaccg
    26401 gcctgcacga ttcggtaatg gcgccgaaat actcggaatg aacgagatat gtcagaaata
    26461 tgggataaaa aatcccaccc agcttgcaga tcttttgggc ctttggggcg atgcttccga
    26521 taatataccc ggtgtgcccg gcattggcga aacttatgcg aaaaaactca ttgcacaatt
    26581 cgatactatc gaaaatttaa ttcaaaatgc cgataagata gaaaacccaa aactcaagaa
    26641 gaaaattctt tctcacactg aagaagcctt attttcgaaa cgacttgcaa ctattcgttt
    26701 ggatgctccc gtaaaatttg acgaggaact ttttaaacgc caggctccag atataaacac
    26761 cttgaagaag atattggatt ttctggaatt tcgtgctttt tcgcagcgaa tattcaagga
    26821 tttgaatcat gataaaccca gtgaacagct ttcatttttt ccagaagaaa cttcaagcag
    26881 gcatgaatcc gatacgaatt tgtttaaaaa tattcatacc actcctcatc aatatctcac
    26941 ccttacttct ttagaggaaa tccgttcttt tgccgatact ctgcaaaaat cttcggaatt
    27001 ttgcttcgat acagagacta ccggcatcga tcccaataat tgtgatctag tgggtatgtc
    27061 gttcgccgtg gtagctcatc gagcatggta tattcctatg cccgtacaac gcgatgaaat
    27121 ccttgatatt ctccagattt taaagcctgt tttccataat cgaaagattt tgaaaattgg
    27181 ccagaacctg aaatttgatc ttattgtgct acaatggtat ggatgcaaca tatacgggcc
    27241 gtttttcgat accatgctgg cacattatgt aatagagccc gaacaacgac atagcatgga
    27301 ttatctcgca cgagcctatc tcaattacga tccagttcct atcgaatcac tcattgggaa
    27361 gaaaggaaat aatcagagaa atatgcaagc ggtggaactc gaaacaatca aggaatatgc
    27421 aggcgaagat gccgacatta cccttcaatt gaagcatgcc atcgaacctc ttttacacca
    27481 gacaagcact tacgagttgt ttcaggaaat agaaatgcct ctgatgcctg tgcttgtgag
    27541 catggaaata gaaggcgtaa aaattgacac cgaagccctc gacgcttatg ggaaacaact
    27601 cgaaaatgag atacataggc tcgaagatga aatatactct ctggcgggtg aatctttcaa
    27661 tatttcttct cctaaacaat taggaatcat cttattcgaa aaattaaaaa tttctgaaaa
    27721 tcctcggctg acgagtacca aacaatatgc cacaggagaa gatgtactcg aaaaattagc
    27781 acatcgtcat cctattgtcg aaaagattct cgattataga gaattaacaa agcttaaatc
    27841 cacttatgta gatacactgc cccagatgat caatccccga actcagcgtg tgcatacaag
    27901 ttataatcag gcggtgactg ctacggggag attgagcagc aataacccaa atttgcagaa
    27961 tattcccgtg cggacagaac gtggccgcga aatccgcagg gcctttatcc ccagaaaccc
    28021 tgattatata ttgatttctg ccgattattc tcagatcgaa ttgagaatca ttgctcacct
    28081 gagtggcgac gaggctatga aagaagcttt caggcaaggc ctcgatatac atgcggctac
    28141 ggcatcacgc atttttaata ttcctctcga tcaggttacc aaagaacaac gacgcactgc
    28201 aaaatcggta aattttggaa ttgtttatgg aatttcggct tatggccttt cacaaaacct
    28261 cgacatagct aaaaaggaag ccgcagctat tatagaagaa tattttaggc aatatcccgg
    28321 tataaaggcc tatatggaaa aaagtattgc gtttgcacgg gagcacggtt atgttgaaac
    28381 catcaaggga cgtaaacgtt atttacccga tatcaattcg gccaatgcca atatacgggg
    28441 atttgccgaa cgtaatgcca tcaatgcgcc cattcaaggc tcggcagccg atatgatcaa
    28501 aattgccatg attaggattc atcaggctct aagtgaaaaa ttcccccatt cctacatgat
    28561 tctccaggtt cacgacgaat tggtattcga tgccagaaag caagaagcag aagaaataaa
    28621 aataatggtg aaagacatca tgcaaaatgc tatgcctctt tccattccgg ttgtggtaga
    28681 tatcaatgcc ggcgaaaact ggctcgaagc tcattaattg cacaaatcat cggtatgatc
    28741 tacatggcca gaatgttgat cgagtaatga ataattcctg gagaagggga aatggtttgc
    28801 gtcatgcagc gagacggaac gaacagccag ctttgcatga aataatgaat gagtcgcgtc
    28861 taaaatggtt tcgggcaaag ttgatgatta agaattctgt tttttgaagg ttaatctatt
    28921 tttttaggtt aacttaaagt aaatcaatta attaataaat ttatcaacaa ttttgtaaat
    28981 tactgttatt caatatttta aatttcatca tttttatata tttatctggt tatcaaagaa
    29041 atatgtttta tttttactag gaaatttaat aagattagtt tcatgtttaa atcaaacaac
    29101 caacaagaaa tattcagttt cgaagatgaa ctcaatcaga agcaaggtga tttactgaat
    29161 agttcgaagg gaaaatggtt ttaccatatc cttttcagca atatcaatga actggatttt
    29221 cgggatttat attcccaaaa ggcaagtcga cctaatgtac cagggaatgt tttggtttgc
    29281 gctttaattt taaaagaact caaagggatc agttatgatg aattgattga aggagtagtg
    29341 tttgatttac attttaaaac ggcattaggg ctttcatgga ttggggatat ccctttttca
    29401 agggcaaccc tattcaattt ttaaaacagg ataatagaat atgagcgtcg gaaaaggaaa
    29461 aaactaattg aacaggtttt tgataagctg acgcaagagc agttaaagga attgttgata
    29521 aatacaaata ttcaaaggag cgattctact ttggtttctt ccaatatcaa aaaatattct
    29581 catttacaat tgctcataga agtattgatg cgattaaaca ggatgctaaa gaaggaagac
    29641 aaggaattta tcggccaaca gatagaacct tatttaaaaa aagggcagat aaatacattt
    29701 atgggttgaa gtccagtgaa ttacctcatg aattagaaaa gttaggtaag atatactata
    29761 ctatattcga atggttgagt aaaaaagagg aatatcattc aaggaaagaa tatatcaatt
    29821 tcactcgggc atacaatgaa cattttgttg tgaagggaga ggaagtgaat ccaaggtctg
    29881 agaaggagtt gacccgtagc atgttgcaat cgcctgatga tgtggatgct acttacagga
    29941 aaaagaatgg ggagcaaagt aaaggatttt cgataaacat tacggaaact gcaaatccag
    30001 ataatcccat acaattaatc actggcatag tggttatgcc caacaatgtt gatgacagcc
    30061 agatattaaa cgaaaggatc gatataatta aagagaagac gccacagttg aatgaaatgc
    30121 acatagatgg tgattttggt agtgaagaga atgataagaa gtttgaagaa ttagggataa
    30181 accatatcac aacagcagtt agagggagag aaagagagat agaaatatag aaataaagat
    30241 agagcagaaa tccaagacgc cggaagtgta tactgtagaa tgtccttttc agaaggtaga
    30301 gtccatgccc acaaagaaac gacataaagt atgttttgct atgaaaatat gcaagggatg
    30361 tttgttaaaa gataattgtt aggcatttaa gaacaatggg tgatattatt taacacataa
    30421 aagagtatct acaaaacaaa cgcagtcgca atattttgag catacctaaa gagcgctgga
    30481 aacttaggcc aaatatagaa gctacggcac gtgaatttaa gataaggaca gaagatggga
    30541 aattaaaagt aagaggatta ataaaggcga gtttatttgc ttatagcagt gggatagcga
    30601 ttaattttgg aaggacatac agatttctgg tcaaaaatgg atgtcctgat ggatttaata
    30661 ggccaataat tgtatttttg aaggatatta tgtcaaatac aggggaaata ttaacaaaat
    30721 gtgaatttat tgttgataat ttttccttgt ttaggtattt ttggttcttc ttgattcaaa
    30781 aaattttttc aggtccaaaa atgtcgaaac aggaaacttt aatccaactt agtttttaaa
    30841 ccggactcaa taatcaaaaa taacaaaatt cgattcataa taagaagctt ttaagggatg
    30901 gagaagaata gcgtcgttat aaatactaat tttttcataa gtaattggat ttaggttaaa
    30961 gatttataaa tcaatgaatt tttattttga cttctttcgt ttgtaaatga ttacaaatgt
    31021 aaatatacaa aaaattatct taaaaatttc atctatttca aaattttcga acatagatgt
    31081 gaaaagattt gaatcctaaa aactgactta tctttgcaaa gacttcaata ttttaaagtt
    31141 cagatgttga acgccttacg tttacaattc tcgctaagaa acggcaagcc tatggctctt
    31201 ttgcttattg cagtcatact ttcggccagt ttgctaaaat cgcaacctcc ggcgggatat
    31261 tacgatggta ccgaaggcct gacaggcgtg gctcttcaag ctaagctgca cgatattatc
    31321 gacgatcacg cttcggtatc ctatggttcg attttgttgc acttccaaac caccgataaa
    31381 aaacccaata acacggtttg ggatatgtat tccgacgtac cgggtggaaa tcctccctac
    31441 atttattatt tcaatgaaga tgaatgtggt agctacaaat cggaagggga ttgtttcaac
    31501 agggaacatt catggcctgc aagctggttt aatgatttaa ttcccatgta cacagatctc
    31561 tttcatattt atcccaccga tggttacgta aacggaaaac gaagcaatta cccttatggt
    31621 gaagtaggaa gcgcctcatg gacttctatg aatggaagta aattagggaa ctgtaattat
    31681 ccaggatatt cccaagtggt gttcgagcct atagatgaat acaagggcga tttggcaaga
    31741 agctattttt atgtggcaac acgttactac aatgaagaca atggctgggc aagcaatgga
    31801 atggtaacgg gctcgcagtt gaaaccatgg gcggttagca tgttgttgga atggcatcat
    31861 caggatccag ttagtcagaa agaaatcgac agaaacaatg ccgtttacaa tattcaaaac
    31921 aatagaaatc cttacatcga ccaacccgat tttgttgata aaatctgggg agaaggcgct
    31981 ggtatcgcgc acgaaatttc tgtgatttat cagctatatc ccaatccttg cttcaacttt
    32041 ataaaaatcg tgttgcccga tgacccgacg gattcttatg ttttcgtcct caccgatatc
    32101 gcagggcgca gaatcttttc aacttcttta cgtggtggaa aggaaaatct catcgatgtt
    32161 tctcatttga aagatggaat ttacattgga cagatcgatg agaatattcc catcagaatc
    32221 atcaagcaat aaatgaatta cattcgtatt acaaagaaat ttaggtttga aatggctcat
    32281 gcgctggtgg gtcatgatgg gccttgtcgt catatacatg gacattcgta tgaactgtgc
    32341 atcacactga agggccagcc gaaaaatgat cctcgttcat ccaaaaacgg aatggtcatg
    32401 gattttggag aactcaaccg catcgtaaaa tctgccctca tcgatcgttt cgatcatgcc
    32461 cttgtattaa atgatgctgt tagggacgag attccacttg gtggtcgtga gttgtttgcc
    32521 agaaccatct gggtagagtt tcagcccact accgaaaatc ttttgcttta ttttgtcgaa
    32581 attttgcggc ctcttcttcc ttctgacata gaactctaca gcttaaaact aagcgaaact
    32641 gccacttctt atgcagaatg gtatgctccc gaccaaaaag ctgattgatt ttttattgtg
    32701 gtttgtatat cagcctaatc agaacatctc ctaccatttg caatgattgt gaatcgacat
    32761 gttcgagatt gtcctgtaaa gtgtgccagt gttcgaaaaa agaacttccc tgtgagtcat
    32821 tttcgagatg aatgatatct atcataggaa ttccggcata tttattcacg tagtaatgat
    32881 cgtcggtaat aaagcctcct ttctgcttgg ggaaatactt accataaccc agggatgcgg
    32941 caatatccca aattttttta accacatcgg gagcataata catggagaat ccttccatga
    33001 gaaaggatgg ttggggcact cccaccatat cgagcagtac gccatatctg gcattatagg
    33061 atggaatatg tttattgcgg ctccaatact gagagccaag gccccaccaa tcctcaccct
    33121 gttgggattg catatcttcg ggaggaccat aatcttcggc atcaaataaa ataatatcta
    33181 ctccgatatt tggtggataa ctatgcatga ttctggccaa ttctaagaga acccccacac
    33241 cactggctcc atcattggcc ccgtcgatgg gtttatgacg attggcagga ttggggtcat
    33301 ggtcggcaaa aggacgcgaa tcccagtgcg aagccaacaa aatacgcgaa gaagcttcag
    33361 gacggaaaga agcaataata ttgcggccat ttattacttc tccattataa gcccttgcct
    33421 tgaatgcttg aactataata tcggaactat agctacccag tttttggatg agccagtccc
    33481 cacaccgcct gtttgcgtca gaattcgaaa cacgcggtcc aaaaccaact tgcttaacta
    33541 cgaaactgaa agcagtatcc gcagaaaaag aaggataaaa cgtagtatct attgcaattt
    33601 ttggtttctt ttcatgttga ggacctttac aggaatacag aaaaatcagg ctcatgcata
    33661 gcatcccaaa atatttaccg attttcatct gtttagaaat tttggttttt atttagaatt
    33721 gataaggaat accaaaatca gaaccaaaga taaaactttc gagagatttt cgtgttttag
    33781 gtccattttc catggttcga agaaaatcgg gtagagtctc aggattaccc atatatccta
    33841 cagcaatgac aacaataggg gcaacctcct cgggaagatg gaaaagttcg gcggttttgt
    33901 cggtatcgaa tcctcccatc tgatggcaga ataaaccttc gtaagttgcc tgaagcgtga
    33961 gataagccac agattgcccc acatcgtagt cccagtaacg attatcggaa ttatctctgg
    34021 agacgtattt tgttcttccg caagccatca taagcaaagg tgcttgaatg gcccaaagct
    34081 gattaaattc tacgagagta gaaaaaattt ttcgataagt ctcatcgtcc tttcttccaa
    34141 tgaagtaata ccaaggttgt tcattcatgg cactgggagc caaacgagca gcttccatca
    34201 tgttctggag tttttcctgc tccactgcct tgtcgtcgaa acatcgaggg ctccatctcc
    34261 tgcctaaaat atccagaatt tccataatta tcaggtttat gcatttttca aatttttatt
    34321 attcaaacca tttataattc actttttttc atttacctat tgtttgaaac aacaaaattc
    34381 tgatatttgc gtaaataacg aaatgatttt catttttaaa tataaaatgt taaaaattaa
    34441 acagaaatga aaaagttatt ttgtgtgatt ttgattattg ctgcaggatt tatgcatgca
    34501 gagttacttc aggctcagag gaactatgga agttatgagc ttgggttgcg gctcggagat
    34561 acttatggag gttcggcagc tgtggatgcc atgcttccgc tagcaggcaa ccgtcttcat
    34621 gccgatctgt cttttcatca cgatggccta attttggccg gattatacga ttggggcttc
    34681 cctattgcac ctggttttat tttctatccc ggtgtgggag gcatcgtata tattggagat
    34741 gtatttcgcc ttggagttac aggagaattg ggattcgagt atgctttcga tattcctttg
    34801 agtattggat tggactggcg tccaataata actatcatag atgaaggagg tttctcagga
    34861 gacggttttg gtttgaacat tcgataccgt ttttgattcg taacataagg ccatgaaaca
    34921 tgatgttttt gagtttgtcg agaaaataat ccatgcggtt agttataact gattttagct
    34981 ggaaagctat ataaacaaaa ggaaaacacc ccaattccaa ctaaattcaa aactcaaaat
    35041 tctaacatct ctatcccata atcttttcaa ctttatagcc atagtcgaaa gaaccttatt
    35101 ttttttcgct gatttacgct gctttaatac gctgatatta actaataact gacaacgaac
    35161 aacaaacaag cccccccaca atcaccatcc accatccacc ttgtctccca gtctcccttc
    35221 gtcctttcct cctttataaa cttttaacgt tctacccccc attctcgata aaaacgaatt
    35281 tttatccgtc agtgtaccga ttgaaaatta tcatgtaaat ttgtggtctt aaatgaaagg
    35341 taaacaacga ttattataat taaaacattg aagatgaaaa agatgacttt tattttaggg
    35401 ttagccattg caagtattat attcttggct ccccaaaaga aaatttgcgc tcaaaccctc
    35461 gaagacgcag gaaacacatt taattctgct attcaactga tgcagaacga tctgccaagc
    35521 gctatttcaa agtttgaaga agtgatcggc atgtgcgaga aaataggcga aagtgggaat
    35581 gaattgaaaa tgcaggccca gaaccagcta cccattctgt attacaatta tgcagtaaat
    35641 ttacaagctt ctcaaaatta tgatcaagca atagaagcat gtaataaagc cattcaaacg
    35701 ggccagcagg tgaacaatgc caatatcatt gcaaaagcgg tcaacttgac tgctcaaatt
    35761 tatatggtaa agggtaacga atacttcaag aacaaagatt ttgcgaatgc gctggtcagc
    35821 tttgatcaag ctattacgct cgacagtacc ctataccgag cttattatgg caaattactg
    35881 gtttataggg agcaagacaa atatgatgaa atggatgaga tgctcgagaa ggtaaaaacc
    35941 accagcactc aggaccccca atttgcagaa aaagctcagg ctctcgtggc cagagagtat
    36001 tccattgccg ctgcaaaagc cattcaagca aaaaatagca gccttgctct tacctattgt
    36061 gacaaagccc ttgcattggg cgatgctacg gctaacactt actattatca aaccttagca
    36121 gcaaatatgg agaaaaaata cagcctggca gcagaagcag ctcaaaaggc actcgagctc
    36181 gaacaaaaag ataagagcaa gatttacttc gaattgggca aggcatacga aggacttggt
    36241 agggccgccg atgcctgtgc agcttataaa aatgttactg atggtccact ggttcaaaat
    36301 gctcaatacc aaataaaaca ggtgctgaaa tgtaaataga acattccacc ttttataatc
    36361 ctaaaactct taccaaattg cgggagattc gaaagagtct tccgcttttt ctgtttttca
    36421 gttaacaacc agctctcatt tttctccggc aaatttcctc tgcttcgtaa gcactgacag
    36481 ggaattacca aaccatcaga ttacagcctc ttgattcggc caagtcatga cgtccaataa
    36541 cctcgaattg ctcaattccc acagaacggc ccaaatctct cgtggctatg aaactgcatg
    36601 aataataatt agccaggtcc actatattaa tcaatcccga tctcccgggt ttggtaagtg
    36661 aaaaaggatc atcagcctca gtaagcaaaa tcttcaacca gggtggagtt ttaaaaatgc
    36721 cttctccttg agaataggct tgagataaca tctccgtcat tccatactcg gaatggattt
    36781 tttccatttc aaaactatgg cttaagattt tatgcaattg tgaacgggtt atttctttgc
    36841 gtcggccctt cattccccca gtttccatca ctataacccc ctgaagattt tgtggaaact
    36901 tttcggccaa atcccataat gcatgagata ctcccatcaa caagactttc ccttctcgcc
    36961 atgttttcgc tttctctact cgctctgaaa gtttctcgac ttcatcgaga aagaagccat
    37021 tatatgggct ctcactaact tccatcaacc ctttcatcat aaaaataagt gaggaattcg
    37081 gccttcccct ataagatggc aataaagtta gaaaagtcca atctgaaggg tttccataaa
    37141 attgtgcaaa cccccttaga aagctttgac ggtaaagttc tacatcgaca acgaaatggc
    37201 gagaaacatt ctggccagta gttccactac tctcgaaaac catctgagga gattgttgtt
    37261 cgacgataac cttctgcgtt ctaaagaatt caatcggtaa acagataatt tccgaataat
    37321 gatggatcgg tatatcgaaa tgatggcttg cctttaaaaa ttctctgtaa actttattat
    37381 gttctttttg aaaatgataa atcgacaacg ccatctggtt gaactcgtct tcattgtcga
    37441 tgttgaaaat atcgtatgga ttcatgatgc aaagatagtt aacagtaaaa agatagctga
    37501 agtgagtgat tacaaagttt tggggtatta tactacccct tgcaatcaaa agcggaggat
    37561 ttgataaagg gatacctctc ggatggattt aaaggttggg gatgaaaagg tcagaaattg
    37621 ggagctttac ttcagaaaga aaacgtatcg aattttcgat atggggatgc atcacttcat
    37681 catattgaat gaaaggaaca tactggcggt atttatggac aaagttttcg atgaggggag
    37741 aactttttgc aggacggcga aattcgagtg cttgtgctgc attgaagagt tcgatggcca
    37801 gaatgcgctg caaattattt accactttca aagccttggt ggctgcatta gcccccatgc
    37861 ttacatggtc ttcctgacct tgcgaacttt cgatggaatc gaccgaagaa ggagtgcaaa
    37921 gctgtttatt ctgattgact atgcttgcag cggtatattg tggaatcata aagccagaat
    37981 gcaggccagg atttactaca aggaagtgcg gtagatttcg tttccctgaa atgagctgat
    38041 aagtgcgacg ttccgagata tttcctaatt cggaaagggc aatagctaaa aaatcgagcg
    38101 caagggcaag aggttgtccg tgaaaatttc cccctgaaat gataaggtct tcatagggaa
    38161 aaatggtagg gttatcggta actgcattca tttcgcacga aaaaaccttt gcaacatatt
    38221 caatggcatc tttactggcg ccgtgtactt gcggaataca acggaagcta taaggatcct
    38281 gcacatgatt cttgggtttg ttcatcagct cactgctctc gagaagattg gcaattcctt
    38341 ttgcagtgga aatctgccct ttatggtttc taatttcatg caaaggcaaa taaaaaggtt
    38401 ctattctgcc atcgaaggca tcgagcgaaa gtgccccaat caagtcagca agagtagaaa
    38461 ggcgaaaact tctcaaaaga caataaactc cataagctcc cataaactga gtaccgttca
    38521 aaagagccag cccttccttc gatttcaaat caacaggact caaaccggct tcgaacaggg
    38581 ctttgctggc aggcattttt tcccctttat aagttacttc acccaatcct aataagggta
    38641 gcgacaaatg agccagaggc gccagatcgc ccgacgctcc caaagatcct tgctcatata
    38701 ccacagggta gattccctta ttgaaaaatt caacgagcaa tttcacggta tctaattgga
    38761 caccgctatg cccataagat aaattctgaa tcttcaacag caacatgagt tttacgatct
    38821 gttcggagac ttcatcccct gttccacagg catgactcat tactaaattt ctttgcagat
    38881 ctttcagttg ctctttcgat atctctatct cgcatagcga accgaagccc gtattgatac
    38941 cataaactgg cccggcatgc atgtcaagtt tgttatcgag ataatccctg caatgttcga
    39001 ctttatggat ggcttcatcc gataaggcaa tctgccatcc ttcctccatt aaatgttgta
    39061 tgttttcgta agtaagttcc ttcgaagaaa tttgatatac cggcatttta tcgtgttttt
    39121 aaaaaatcga tgaatgagat ttcgttagtc cctcgcccgc aaagcgcaaa agaggagaaa
    39181 aatgaatgat aaatcttaaa gataaggtcg aagacaaatc caaaaacgac attcctccta
    39241 tcaaatggct taaaaaagac ccgctcgatt gatttttatc ttatatgtca gaaaataacc
    39301 gtacatgatg cggcaaaatt acgcttttta ttgtttctcg gacgaattgc agcttatttc
    39361 atacaccaat tcttactttg tatatctgca cagtaaacta atgaaatgat aataaatggg
    39421 tcatgttttt tagcgaagct attcttaaga ctatgccggt aaaatgaaat gtttattgca
    39481 gtatttttcg aaagaatcgg attctatgct cagcaatttc taaatttgca tgagtcttga
    39541 aatgcatcga ggaatttgct ttgaaagaaa ataaataaaa atatgtttca gaaatatata
    39601 aggcgtttcg gtattggaat tctatggaag tctataatgc gttctttctt tgtttttatc
    39661 ctgcttggtt tcatgctttc gacttattca ggagtaaggg ctcagaaagt atatggaggt
    39721 ttggtagcag gcgtcaatgg cagccaggta gcaggcgacg tagcatcggg ttatgataag
    39781 gcaggaattc atgttggtct tttttcctat acccatatca gcgaaaagtc ggtttttcag
    39841 atggaactgg tttatagtca gaaaggtgca cgaaaaaacc cgagtgaaaa gaatggatat
    39901 cagcaatata ttctgcgttt gaattatatt gatattcctt ttatttacaa atatttgtac
    39961 aatgatcgtt tgagtttcga aggcggtcta tcgtatggtt atctgctcca ccattacgaa
    40021 gaagccaatt tcagtttgaa tgtcggtgat gctggatttc atacccatgc actcaacatt
    40081 ttggtgggtc taaattatag atttgccgag aaatgggcgg caggtttcag aagcaccaat
    40141 tcactctacc agattcgcga tcatacctcg ggagccagtc gtttcccaaa ctacggtcaa
    40201 tataacgatg tacttaccct caccattcaa tatcttttgt ggagagaata gattcagcaa
    40261 gttgcccttc ttccttttat caaagatgtt acggcaagac tcagtcaata aaatagaaaa
    40321 taaaacaaag aagagctcga ataatttttc cattcgatga gtcagtaaat gaccttcgaa
    40381 atttgttgaa ccctcaaata accaaagctt aatgacaaga atatgcaata aattgaagag
    40441 tagggatcgg ttaaaaatta taaaacttaa tgaagtattt gcaaagattt cgaaaaattc
    40501 gtttcttcgc tgtaagaaaa acctaattct atggaatcat taattgtaaa tggcgagggg
    40561 ttaactatag aagatatagt agctgtagct cgacatcacc agaaggtagt tttgcatccc
    40621 gaagcaattg agaagatcgt gcaatgccgc gccatgctcg aaaaaaagat cgaagctcgc
    40681 gaaatcatgt atggagtgaa tactggaatt ggcgagtttt ctgaagtggt actgaccgat
    40741 gatcaggtta aggattttca aaagtattta atatacaatc atgcagcagg gataggggaa
    40801 cccatgcctg aggagtgggt cagaggagcg atgttgggtc gcatcaatgt gcatgcacat
    40861 ggcaattcgg gatgtcgtcc agagattact cttacgatgg tagagatgct caacaaaggt
    40921 gttacacctt atgtatgtac gaaaggttcg gtaggtgcct gtggtgattt ggctcccatg
    40981 gcacagatag ctcttttgtt gatgggagaa ggtaaagcct attatgaggg agaattatta
    41041 gatgggaagg aggccatgcg aagggcaggc atagaagtac cgggcttaaa ggcaagggat
    41101 gggctggcgg ccataaatgg gagcaatgta cttacggcca tgagtgcaat ttttttatat
    41161 gatgcaaatc gctggctgaa gcaagcagag attgccactg ccatgtcgct tgaagcgcta
    41221 aaagccaacc tgaaacctta tcatgaattg atacatcagg taagaggttt cccgggtgcc
    41281 atgagaagtg ccagagccat tcgaaaaatg gtcgaaggag gcgatctggc cgaaggccga
    41341 ttaaaatgta aggtacagga tgcttattcg atgcgttcga cgccacaagt gattggagcg
    41401 gctcatgatg cacttgctta tgcacgttca caagtcgaaa tcgaattgaa tggtgtaggt
    41461 gataatccca ttttctttcc cgaactcaat cttcagcttt cgggagctaa ctttcagggc
    41521 agtccggttt cattacccat ggatatggca ggtgcagcca tcacaatggt ttgtgtgctt
    41581 tctgaacgaa ggctcaaccg actcaataat cctgccctga gtgttggttt acccgctttt
    41641 cttaccaaag gtgccggaat gttctctggt cttatgctta gccaatacac tgccgatatg
    41701 cagattgtgg aacaacgcat tctttcgatg ccagcttcca tccagtctat tccggcagcc
    41761 gccgatcagg aagacttcgt gtctatgggc atgaacacgg ctttgaagaa tttccagata
    41821 atcgataatg cctacggaat tcttggtatt gaaatgatgg ctgcagcaca ggctctcgat
    41881 tttagagatt ataaattcgg tactggtaca acctgcgcca aggaaatcgt acgccaatac
    41941 gttgaatttc tggatgtgga caggccttta ttcaacgatc ataataccat gaaaaaactg
    42001 gtggaatcat gcgaaatcct cgaaaaagtg gaaaataaga taggaagcct gagttagtaa
    42061 tgggggtttt tatggagatt cgggaaaata ataccccatc acaggagaaa cggccaaaaa
    42121 ttctcaccat cctttgtatt ctatcattca ttggaagcgg gatgggaatg atagcctacc
    42181 ttttctatgg attgatgttc aacgaggttg aaacaatgat agaagaagga caatttgctt
    42241 ttccgggaat cgaaatcctg atgagtggag gaaataaata ttttctgatg ggctctttac
    42301 tttatttgat ctcctttgcg ggagttcttt tcatgtggaa aatgcgtgca ataggatttc
    42361 atctttatac agcagcccag attctaatgc ttattcttcc ttttttagca ctcgagaatt
    42421 atcctgtgcc attcgtcgat tttatcatta cccttatctt tattctttta tatcgcaaat
    42481 ttttgccgat gatgaattaa aatcatccgt aatatatgaa atctgaactt attagcgcat
    42541 atcctatcaa ttttttaatc tatttagaaa atactataag ataaaatttt atggaaatat
    42601 tttatggaaa tggttcaatt ttgtaaaaaa tgttataaat tgcacccctg aaatgaaaaa
    42661 ttgaaaataa caccaagaaa ttttagtttc caaaacctta tcaaacgttc ttttcaagtt
    42721 cattgaccta aactttatta ctaaccaaaa tctaaccgtt atgagaaaga ttcctttttt
    42781 acttgctttc ttagctttgc taggattgca ggtacaggct caaaggcagg ttactggtac
    42841 cgtaactaat gctgaagatg gttccagtat tccgggcgtt tctgttctcg taaaggggac
    42901 acaggtggga acgattaccg atatcaacgg taaatactcc atcaaggtgc ccctcgaagg
    42961 taaaatcctt caattttctt tcgtaggcat gcagacctta gaagtaacca ttggcaatca
    43021 atcggttatc gatgtacaaa tgcggcctac tactactgcc ctcgagggtg ttattgttac
    43081 tgctttagga atttcacgtg agaagaagtc attgggttat gcagtgcaag atgtgagcgg
    43141 agatgaaatt tcacaggcgc gtcagctcaa tgccatcaac tctctttcag gccgtgtagc
    43201 aggtttgcaa ataaccagtg gctcaggaaa tatgggaggt tcttcacgcg tattaattcg
    43261 cggagtaaac tccatttcgg gcaataataa tcctctttat gttatcgatg gtgttcctat
    43321 cgataacagc gatttcaaca cggtgaatac agctagaggt gctgggggtg tggactatgg
    43381 taatatggcc caggatgtca atagtgatga tattgagtct atctccgtat taaaaggtcc
    43441 agctgctgct gctctttatg gctcacgcgc tgccaatggc gttattctta ttactacaaa
    43501 aaaagggaaa gctggagctg ctaaagggat tggcgtttct atcaattccg gcatttcgat
    43561 cgaaaatgta gcttatcttc ctaaatacca aaatctttat ggtggaggta caacttttac
    43621 aggagcaggg acaaaggacg gtttcttagt acaagaaatc aatggtattg agtatttgct
    43681 tcccgactat gctactgatg aaagctgggg cccgcgttac aatcccaaca tttattattt
    43741 gccctattgg agtctttatg actgggaagc cttgggcaag cctacaacca atgctgatgg
    43801 tacaccctat cgtttagaaa ctgctccctg ggtagctcct aaacacgatg ttgaagactt
    43861 cttcgaaacc tcggtaggat atactaataa cattgctttg accagcgcca gcgaaaatgg
    43921 ttcattccgt ctgtcgtata ccaatttctc ttccaatgga tatatgccca actctaccct
    43981 caacaggaat accttcaatt ttaacggaac atcgaagttt gggaagaaac ttatggcatt
    44041 cacttcattt aattatatca agacttatgc tctgggaagg cctgttactg gatacgacga
    44101 taacaatgta ctgcagaaat ttacccagtg gggtcagcgt caactcgata tggaaaaaat
    44161 gaaaaactat atgaaccccg atggaaccca acgtacatgg aaccgcagag catggtttga
    44221 tccattgcct aattattcgg acaattttta ttggacacgt tttatgaatt acgaagaaga
    44281 ctggcgtgat cgttattatg ggaatgttgg ccttacctat caaatttttg attggttaaa
    44341 attgacgggt aaatataata tggacactta cacatttcgt atcaacgaga gagtagccat
    44401 aggaagtcag gccttatctt actatcagga agacgttaga cagctgaccg aaaccaatgc
    44461 tgagtttttg ctggaagccg ataaacgttt tggtgatgat tttaccacta aacttacctt
    44521 aggagggaat cgcatgcacc gccagtacga gctcaatggt ggtatcacac agggtggctt
    44581 ggttatcagg aatttctata acttgagaaa ttgtctttct ccggcactcg ttcatgacga
    44641 aacatcacaa aaatccatta attctctcta tggcagcttg aatctgggtt ataaaggcct
    44701 gttatatctt gatgtaacct tacgtaatga ctggtcatct acattgccta aaaataactg
    44761 gtcatatcct tatccttccg taacccttag ttatgtattc agtgaaatgc caggattgca
    44821 gaatcaaaac tggttcgatt ttggcaagtt gagattcggg tgggctgaag taggtaatga
    44881 tactgagcct tatcgtttaa tcaccttgta tgaaaacatt cagaattttg gaagtgatat
    44941 tatttacaca cttcccggca tgctcaacaa tgctaaatta aggcctgaaa gcacgaatgg
    45001 ctgggaaatt ggtactgaat tgaggttcct taacaatcgt attggtctcg atttcactta
    45061 ttattacaag aaaactactg accagatttt caatactccc atttctgctg ccagtggttt
    45121 tacctccgcc atgattaatg ctggcagtat ttccaacaaa gggatagaat tcttactcac
    45181 gcttacccct gtaaaggtga aagattttac atgggatatt cttttaaatt ttgccaagaa
    45241 caccaacaag gttgaagaac tcatcgaagg tgtcgaaacc cttcgtttag ccaatgggcc
    45301 cttcaacatt tcagttgaag ctcgcgtagg tgaagcttat ggacagcttg taggtactga
    45361 ttttgtctat gatgcaaagg gaaataaggt ggttggaacc aatggacgtt atcttaaaac
    45421 tacggtagtt caatctctgg gaagcgttct ccccgattgg aatgctggca taaccaacgc
    45481 tttcaaattc aaaggcttcg acttcagcgc tttgatagac atccagcatg gtggcaaaat
    45541 attctccacc tcatacatgt ggggtattta tagtggtatg ctcgaagaaa cggctgctat
    45601 caatgccaat gggaaaaaca tccgcgatgc ggttgccgat ggcggtgggg ttctgataga
    45661 cggggtttat ggaaaactca atgccgacgg aagcgttact tatcttgata aagatggcaa
    45721 ccctgtggat tctcctgtaa agaacgaaac ctatatctcc ggcgtacgct gggctgctga
    45781 ccattactca ggccctgctg cacagaatgt tttcgatgcc tcttatgtaa aactccgtga
    45841 actgcgctta ggttacacta ttcctaacag atacacgggg cccatacaga atcttcgaat
    45901 ttcgatctat ggtcgcaacc tcgccatttg gggcagagac aataaacaca tagatccgga
    45961 atatactacc agctcaggta atactcaagg cattgaaggt gggcaactcc cctcgcttcg
    46021 ctcctttgga attaacctaa catgcaattt ctaactgttt ctaaaaaatt taaattcaaa
    46081 tgattatgaa aacattacat aaaattgccg ttgttttcaa cctgccttta ttcattctcg
    46141 caggttgtac aaaggatttc gaagaaatca ataccaaccc caaccgtcct atcgatgtgc
    46201 ctagctatgc ctttatgacc agcgctcaaa agcaactatg cgacaacatc ttcgatgaat
    46261 ggtggggtgg ccgtcaaagc atgctctggg ctcaatattg ggctcaaaat aactacaccg
    46321 atgaagatcg ttacaatatc cgtcagaatg taaacgatgg ctattggaga acaatttaca
    46381 ccattatggt agatctcaac gaaatcatcg agttgaacca aaatcccgat aaaaaggatc
    46441 tgctttcggc ttatggagcc aatgaaaatc aaattgcttc ggcacgaatc ctccgcgctt
    46501 gggtgttcca acatcttacc gacctttatg gagatatccc ctattttgaa gccctaaaag
    46561 caaaatcaga tagagtattt ctaccacctt atgacaagca gagtgatatc tatcctgcta
    46621 ttattcaaga actcaccgag gctgccgcac aaatcgatgt tacaaaagct ggttggacgc
    46681 agggtgatat tatttataag ggtgatatga caaaatggaa aaaatttgcc aatagtttga
    46741 agctacgtat ggcgttgaga atgaaaaaag ccgatcctac caatgcacag aagtacatag
    46801 atgaagcgat tgcgggagga gtatttacca gcaatgggga tgttgcagcc tttaaatatc
    46861 ttccatctgc acccaatact gcacccatgt attctgccta ttacatcgat aatcgtaccg
    46921 attttgcggt tacagcgcag tttgtagatt tacttaaggg aatccctgta ggtggtgctc
    46981 aaaaacccaa tccttttgct gggttggaag atcctcgtct gccaattttt gctgatcccg
    47041 tgaaagattc tactatgctt ggacccggtt atcagggaat gccttatgga atgcctgacg
    47101 aaatcaccaa ggctatagga gctcagggtt cttcatggcc aggggaactc atccttgccc
    47161 ccgatttcgc tacaatctta atggattatg ctgaagttga atttattttg agcgaaatca
    47221 atggatggga tcagactcac tacatcaacg gagtaaaggc ttcactcgac tattggggag
    47281 tggatgccgc tatacgcgat gccttcttgg cagccctccc gccggcttca caagaaacag
    47341 tcctcaccca gaaatatatt gccctttata cccaaggtta tgaaggctgg agtgaatacc
    47401 gtagaacagg ttatccaaaa accctcgttc atccaggtga agttacttgg atcgatctta
    47461 gcagtaaccc cgttaaattt gaagccctta caggtgatga cattccacgc cgtctaactt
    47521 atccacagga agaacagaaa gtaaatgtca ccaattataa agccgctgtt gctcaatttg
    47581 gtgaagatgt cttctcaacc cgcatgtggt gggataaacc atgattccat aaaggaacaa
    47641 caacttttat taatcgagag ggcggctctt tgggccgccc tctcgtatta attatcttct
    47701 ataattcatt aattaataat tagtaagaaa cgaaatccta gcgtatcaac tatccattca
    47761 tcctaatatc ttttcttgaa tttctcatag aactcaaata aaacctcagg attccatgtt
    47821 cgttttacat gctactacat tatccgttaa gccattttat tttcgataat gtttcgaatt
    47881 ttctttaatc attgttggat tttcctataa atattaattt tgcttcgatt tgttcacaat
    47941 tatatgtgat ctgtttttca tttctcaaca aagaaaaata aaattttaga gtgtgtaaaa
    48001 tttagataaa atgataaagg taaattcatt gtgaaagaca tgagaagtga attgcaaact
    48061 gatcgattag ctctttaatt catcataacc tatggattgc acgatgttta tacaaaattc
    48121 gttagctatt aatcaacata aattcagaat ataaaatgat tactccatct gtttctaatg
    48181 atcaaactac ttcttttaca gaaagagact tacaacaaat gttcgatagg ttcgatttta
    48241 ccgatgaaac ccgaaatatc gtagagcaag aaaatttact tccgttaggt ttaattcgcc
    48301 gacttcattg tgctttccca aagtactctg ctttcagaga agagagacct tttatgttca
    48361 aacagaaaag gcaatatata gggcttgagg ggtttagaga aatcgtcagg attcttcagt
    48421 cgaacggagt taaaatggat catctcgaag aaagggaatt gttcatcaag gtatatcgtt
    48481 ttatggccac tcgccatgtg ctcaatatca tcaactggga caatccttct atggattccc
    48541 tgtttcaact ggtttttcct caaccgggga tgattaggaa ggaaatagta gatgcatact
    48601 tcgcagcagg taccgatgcc gaaaaggaaa aaataatctc tgactacatt cgccatactc
    48661 acccacatga tggtaagcaa ttgcttaata agcctgtatt cgaaaacgaa gagggcaaaa
    48721 tggaagtgct cgagggatgc caacacaaat atccacagtg tgaattgatt tttgatgtac
    48781 aaacccaaaa ctgctttgct ttctgtactt attgtttccg tcatgctcag gttagaggcg
    48841 atattgacat gtttctacag agagatattt cgcagattca tcgttattta cgcaaacaca
    48901 aagaagttac tgatattttg attaccggag gagacgccgg atatctgaaa gccgatcgtt
    48961 tcgaaagcta cattacgcca attattgaag accctgaatt aaatcacatt cggacggtac
    49021 gtattggcac acgcgtgctt acttattttc ctgaaatgat tcttactcac gattacgaaa
    49081 atatgttgaa tttatatcgc cgattgatag acaatgggat acagattctt ttcatgtctc
    49141 atttttctac tccccgtgaa atcatgaatc caacgactgt ggctgctatt cgtcggttgc
    49201 gcaattatgg agtaactatg aagagccaaa gccccatcat gaatcatatt agtctgttta
    49261 ccgacgaaaa tgggaaagtg gatatagacc gttcggctca aaattggata gatttgggca
    49321 atttattggc tatgctgggt gttggcttcc attctatgta tatggctcgt ccaacgggcg
    49381 aacatcatta tttcacagct cctttggccg atatcaataa catttttcag aaagtttatc
    49441 gttctctggc atccatcaat cgtccgtcgc gttttattac tatgacatct tcagcaggaa
    49501 aaatctcgat gttgggtacg attgatattg agggagaaaa agccttcgta ctgaaaatca
    49561 atgaaggccg caatatggaa tggatggata aaatatttat cgctaaatat gatgaaaagg
    49621 aaacggtgat cactcgtctg aaacctttct ggggtgataa attcttctat gaagacgagc
    49681 tggccgagat cgaacatcag ctcgaaggaa aactgcattt tcatcttgaa tagatgattt
    49741 tctaaaaaca tgatgaaatg atcaataaag tagtagttac tgctgcagag gcagtcgcag
    49801 atatttttga cggtgctaaa attatgattg gtggatttgg cgaagcaggt agccccatcg
    49861 aactgataca tgccttgatc gaccagggag ctaaaaatct gacagtcatc agtaataata
    49921 caggtaatgg gcatgtggga ttggctgcat tgatcgaaaa tcttcaggta aagaagatga
    49981 tttgttcctt tccacgcact gcccaatcgg tagtttttcc tgaattatat gctaaaggga
    50041 tgatagaact ggagcttgtg ccacagggta cacttgctga acgaatacgc gcgggcgggg
    50101 ccggaatacc ggccttttac acccccacca ccattggtac tccactagca gaaggcaaag
    50161 agatacgaca tatcgatggg aaatcctacg ttctggagta tgctctcaag gccgattttg
    50221 ctctaatcaa atgcaaaacc gccgatcgtt atggcaatct gctctataac aagacagccc
    50281 gaaatttcgg ccctattatg tgtatggccg ctaaaacaag cattgttcaa acttcgagtt
    50341 tagtcgaacc tggcgaaata gaccccgaaa atgtagttac acctggcatc ttcgtgaaac
    50401 gagtcgtaaa aatttcaaat ccattgagtg agaaccaatt actcagggag aataggaggt
    50461 atccatggta aatataaaag gatggagcaa tgatgaaatt gctcgcaaaa ttgccatcga
    50521 tattcccgat ggttcttatg taaatctggg cataggactt cccgaactgg tggctgctaa
    50581 tattcccgaa ggaaaagaga ttatctttca tacagaaaat ggattattgg gggtaggacc
    50641 tccaccacct cccggacagg aagacgacga actcatcaat gctggcaaga aacccgtaac
    50701 ggctatccca ggagcatgtt ttttccatca tgccgacagt tttgccatga ttagaggagg
    50761 gcacattgat tactgtgtat taggtgcttt tcaagtatcg gaaagtggtg acctggcaaa
    50821 ttggagcacg ggcgagctta atgccatacc tgccgtgggc ggcgccatgg accttgtggc
    50881 tggcgttcct aatattttca tcatcatgca gcatattaca aagaccggcc agcctaaaat
    50941 agtgagacaa tgcacctatc ccctcaccgg aaggcaagtg gttaaaacca tatataccaa
    51001 tctggctgtg atagatgtaa cccctcatgg actcgaagtg agggaaattc attccggcat
    51061 ggatagagaa actttacaaa atctcaccga agccagtctt agttttcgat catagtttta
    51121 ttcataaaat ttacgattat catggattca ttgaaagagg aaaaaaattt agaagaaagc
    51181 ctttatgagc gtgccctgaa ggttcttcca ggaggtatca gcc
//
LOCUS       DAOORU010000003        47169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_845_length_47169_cov_5.589994, whole genome shotgun sequence.
ACCESSION   DAOORU010000003 DAOORU010000000
VERSION     DAOORU010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 47169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47169
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_845_length_47169_cov_5.589994"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..496)
                     /gene="rplE"
                     /locus_tag="PLF20_00480"
     CDS             complement(<1..496)
                     /gene="rplE"
                     /locus_tag="PLF20_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOX79519.1"
                     /translation="MDYIPRLQKKYREEIIPALVKQFNYKSVMQVPRLVKISLNQGLG
                     DAIADKKLIDVGIEEMTLIAGQRAIATKSKKDISNFKLRRNMPVGVKVTLRGARMYEF
                     LDRLISIAIPRIRDFHGISDKGFDGRGNFSFGISEQIIFPEINIDKVNKIMGMDITFV
                     TSAET"
     gene            complement(496..819)
                     /gene="rplX"
                     /locus_tag="PLF20_00485"
     CDS             complement(496..819)
                     /gene="rplX"
                     /locus_tag="PLF20_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOX79520.1"
                     /translation="MKPKLHIKKGDVVMVITGEDKGRQGRVLEVHVEKQKAIVEGVNL
                     VLKHSKPNAKTPKGGILKREAPVHVSNLMLIGPGGKPTRIGYKIDPKTNKKVRYAIKT
                     GEVIK"
     gene            complement(839..1207)
                     /gene="rplN"
                     /locus_tag="PLF20_00490"
     CDS             complement(839..1207)
                     /gene="rplN"
                     /locus_tag="PLF20_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOX79521.1"
                     /translation="MIQQETRLNVADNSGAKEVLCIRILGGTGHRYATVGDKIVVTVK
                     KALPSGTIKKGTVTKAVVVRTKKEIRRADGSYIRFDDNACVILNNTGEMAGTRIFGPV
                     ARELREKQYMKIVSLAPEVL"
     gene            complement(1210..1467)
                     /gene="rpsQ"
                     /locus_tag="PLF20_00495"
     CDS             complement(1210..1467)
                     /gene="rpsQ"
                     /locus_tag="PLF20_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOX79522.1"
                     /translation="METRNLRKERIGVVVSNKMDKTIVVKVERRLMHPKYGKFVKRST
                     KFKVHDQNNDCNVGDVVRIMETRPLSREKCWRLVEIIERAK"
     gene            complement(1471..1680)
                     /gene="rpmC"
                     /locus_tag="PLF20_00500"
     CDS             complement(1471..1680)
                     /gene="rpmC"
                     /locus_tag="PLF20_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOX79523.1"
                     /translation="MKQAVIKELSTNDLVERLEEERKKLTRLRLSHTVSPLDNPMKIR
                     QNRKTIARILTELKKRELEKNKKTS"
     gene            complement(1714..2136)
                     /gene="rplP"
                     /locus_tag="PLF20_00505"
     CDS             complement(1714..2136)
                     /gene="rplP"
                     /locus_tag="PLF20_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOX79524.1"
                     /translation="MLQPKRTKYRKMQKGRMKGNAQRGYEIAFGSFGLKALEQTFITG
                     RQIEAARQALTRHMKREGQIWIRIFPDKPITKKPAEVRMGKGKGAVEYYVAPVTPGRI
                     LFEADGIPLEIAREGMRLAAQKLPIKTKFVIRPDYLEE"
     gene            complement(2169..2888)
                     /gene="rpsC"
                     /locus_tag="PLF20_00510"
     CDS             complement(2169..2888)
                     /gene="rpsC"
                     /locus_tag="PLF20_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HOX79525.1"
                     /translation="MGQKTNPISLRLGIIKGWDSYWYGGRQFDQKLIEDFKIRKYLNA
                     RLSKAGVSKIMIERNLKLITVTIHTARPGIIIGKGGQEVDKLKEELHKVTGKEIQLNI
                     SEIKRPDLDAYLVANNVARDLERRISYRRAIKMAISNTMRIGAEGIKIQISGRLGGAE
                     IARREMFKEGRIPLHTLRADIDYARDEAHTTYGRIGIKVWICRGEIYGRPDFFATSSA
                     TRQQRSPVPGTSRPRSRGKRK"
     gene            complement(2916..3344)
                     /gene="rplV"
                     /locus_tag="PLF20_00515"
     CDS             complement(2916..3344)
                     /gene="rplV"
                     /locus_tag="PLF20_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOX79526.1"
                     /translation="MGARKRRSAELRKAENKLKTSARLAKVSGSPRKMRLVADLVRGM
                     QAEKALIVLHTMKQEGASQIYTLLKSAIANWQQKHEGMRMEDAQLYIQTITVDGGRIL
                     KRLRPAPRGRGYRIRKRSNHITLVLGDQLSNKNEIEEITD"
     gene            complement(3351..3623)
                     /gene="rpsS"
                     /locus_tag="PLF20_00520"
     CDS             complement(3351..3623)
                     /gene="rpsS"
                     /locus_tag="PLF20_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOX79527.1"
                     /translation="MSRSLKKGPYIHYKLEKKVLHQVESKKKNVIKTWSRSSMISPDF
                     VGQTIAVHNGNKFIPVYITENMVGHKLGEFAPTRTFRGHAGSKAKK"
     gene            complement(3645..4469)
                     /gene="rplB"
                     /locus_tag="PLF20_00525"
     CDS             complement(3645..4469)
                     /gene="rplB"
                     /locus_tag="PLF20_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOX79528.1"
                     /translation="MALKKFKPITSSQRFKVISAFDEITTQNPEKSLLTPVKKSGGRN
                     NTGKMTMRYLGGGHKRMYRIIDFKRDKELVPAIVQSIEYDPNRSARIALVEYQDGEKR
                     YIVAPNGLKVGQSIMSGKGISPDIGNTLYLSEIPLGTIIHNIELRPGQGAKMARSAGS
                     YAQLMSRDGKYAVIKLPSGETRLILQSCKATIGMVSNPDHSLEVSGKAGRSRWLGRRP
                     RTRGVAMNPVDHPMGGGEGRASGGHPRSRKGIPAKGYRTRKPKKASSKYIIERRKK"
     gene            complement(4475..4768)
                     /gene="rplW"
                     /locus_tag="PLF20_00530"
     CDS             complement(4475..4768)
                     /gene="rplW"
                     /locus_tag="PLF20_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOX79529.1"
                     /translation="MSSIIVKPLITEKMSIQGEKLNRYGFIVRREANKIQIKKAIKEL
                     YGVDILEVNTMIFTGKNKYRYTKSGIIAGRTRAYKKAIITLAEGEKIDFYSNI"
     gene            complement(4787..5425)
                     /gene="rplD"
                     /locus_tag="PLF20_00535"
     CDS             complement(4787..5425)
                     /gene="rplD"
                     /locus_tag="PLF20_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008623141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOX79530.1"
                     /translation="MELTVYNYKGEDLGRKVILNESIFNIQPNDHVIWLDVKNIQANQ
                     RQGTHKTKERSEMSGSTRKLKIQKGTGGARAGDINNPLFVGGGRVFGPRPRDYHFKMN
                     KKTKRLARLSALSYKALENRIIIVEDFTFDAPKTKTYAEFLKNFKLNDQRSLLVVNEP
                     DQNLLLASRNIPYAKIMRAVDLNTVDILKANRMIFFESSLPVLENLYAESEN"
     gene            complement(5416..6045)
                     /gene="rplC"
                     /locus_tag="PLF20_00540"
     CDS             complement(5416..6045)
                     /gene="rplC"
                     /locus_tag="PLF20_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOX79531.1"
                     /translation="MSGLLGKKIGMTSIYDESGKNMACTVIQAGPCVVTQILTKERDG
                     YDAIQLAFDDLDERKVNKPMRGHFAKAGITPKRIMAEFTRFEAGHRKSFGEVIGVEVF
                     TVGEFIDVSGLSKGKGFQGVVRRHNFHGVGDATHGQHNRLRAPGSLGASSYPSRVFPG
                     MRMAGHMGNKKVKMINLKILRIIPENNIIVVKGSVPGANGSYLKLERWS"
     gene            complement(6078..6383)
                     /gene="rpsJ"
                     /locus_tag="PLF20_00545"
     CDS             complement(6078..6383)
                     /gene="rpsJ"
                     /locus_tag="PLF20_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOX79532.1"
                     /translation="MNQRIRIKLKSYDYNLVDKSAEKIVKTVKQTGAVVSGPIPLPTH
                     RKIFTVNRSTFVNKKSREQFQLCSYKRLLDIYSSTSKTIDALMKLELPSGVEVEIKV"
     gene            complement(6409..8514)
                     /gene="fusA"
                     /locus_tag="PLF20_00550"
     CDS             complement(6409..8514)
                     /gene="fusA"
                     /locus_tag="PLF20_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOX79533.1"
                     /translation="MTERLIYTRNIGIMAHIDAGKTTTTERILYYTGVNYKMGEVHEG
                     TATMDWMIQEQERGITISSAATTVFWHYNQEQYKINIIDTPGHVDFTVEVERSLRILD
                     GAIAIFCGVGGVEPQSETVWKQANKYGVPRISFVNKMDRSGADFFEVISQIKSKLGAK
                     PIPIQLPLGEEEDYRGVIDLISCKAYEWNDTSLGTNFYEVPVPEQLFPLVEKYHNELI
                     EGTAEESDQLFEKFLSDPHSITEEEIKNALRKATIEMRITPVLCGSSLHNKGIQFLLD
                     AVAAYLPSPLDIPAIKGINPLTGEEESRHPDPDEPFAALAFKIASDPYVGRIVFFRVY
                     SGSLKSGNVVLNVATGKRERIARIFQMHANKQNPLEKIEAGDIGAAAGFKQIRTGDTL
                     ADEKHPILLESITFPEPVISMAVEPHTQDELEKMMNALQKISEEDPTFFVGIDEETGQ
                     TLISGMGELHLDIILDRLHREYNVECNHGKPQVAYKEALTRNVRHHEIYKKQTGGRGR
                     FADIEFEIGPADIGVKGLQFINEIHGQNLPKEYIPAIEKGFRSAMSNGPLLGFPIESM
                     KVRLIDGQAHPVDSDALSFEIAAISGFREAIRKAECILLEPIMKLEVVTPDEYVGDIT
                     SDLNRRRGQLLKIDARISYQIVDALVPLSEMFGYVTVLRSISSGRATSSMEFSHYATC
                     PPEIQEQVILKIKGYIIRQ"
     gene            complement(8561..9028)
                     /gene="rpsG"
                     /locus_tag="PLF20_00555"
     CDS             complement(8561..9028)
                     /gene="rpsG"
                     /locus_tag="PLF20_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOX79534.1"
                     /translation="MRKAKPRKRIILPDPKYNDTMVTKFVNNIMLTGKKSIAFDLFYE
                     AVDIVSQKTGEDGLEVWKKALANVTPSVEVRSRRVGGATFQIPTEIRPTRKMSVGMKN
                     LISYSLKRHEKSMAEKLAGEIMAAYKEEGAAFKRKEDIHRMAEANKAFAHFKF"
     gene            complement(9074..9493)
                     /gene="rpsL"
                     /locus_tag="PLF20_00560"
     CDS             complement(9074..9493)
                     /gene="rpsL"
                     /locus_tag="PLF20_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004291249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOX79535.1"
                     /translation="MPTIQQLIRRGRQKLEYKSKSPALDSCPQRRGVCTRVYTTTPKK
                     PNSAMRKVARVRLTNGNEVTAYIPGEGHNLQEHSIVMIRGGRVKDLPGVRYHIIRGAL
                     DTAGVEGRMQRRSKYGAKRPKTKPGAASPKDTKAGKK"
     gene            10158..11336
                     /locus_tag="PLF20_00565"
     CDS             10158..11336
                     /locus_tag="PLF20_00565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX79536.1"
                     /translation="MTEEKLRILFVSRWYPHKEDPMEGLFVQRHAQAVSNYANVSVLY
                     AHPIINKKILSPRWEFSTFRDVDELRIYYSGLSRNFPGSQIIKASQYLFHLIAGYLWL
                     QKRTKRFKLVHVNILTRTAIFASVLKNLTGLPYLITEHWSRYLPITNTYRGILRKIVT
                     RYVVRRAGAITVVTENLKNAMQRHGLHHKRFYIIPNVIDTNIFTPTFLNHSSKKLLHV
                     SCFEDKSKNISGLLRATQKLSHLRNDFELVLVGDGQDFQKIKEYSQNLHLEAYVTFTG
                     LIEKEEKLAECYRQSCCLVMFSNYENMPVVINESLACGKPVIATRVGGIPEIIDSAKG
                     ILIDPKNEDSLCEAMNYMLDHASEYDSQALRNYAVENFSFSKVGECFLKIYQEIKPIQ
                     "
     gene            11333..13027
                     /locus_tag="PLF20_00570"
     CDS             11333..13027
                     /locus_tag="PLF20_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79537.1"
                     /translation="MTLGERKGLKFGILCDTLHLQQWQTLVIEQLILEGNALALIILN
                     KEDSPPKSKGIRRLLCYPIKNFIFLFYYRFLLKPKSKRMLSIEPWIKEAILLEENAKK
                     VKKSYYFSEKTIEAIKSSEVQFLLRFGFGIIKGDILHAAPYGVWSFHHDDPEVIRGVP
                     SNFWEILEDIPVNGAILQQLDESIDCGKILWQGWFPTINHSWKANLDQAYYGSVEWPA
                     QTCREILFKGADCLKNKPFPKPAPMRFAPINCIMLKFFLKLWANKVKFHFNELFRAEH
                     WSIGFIESSIKNFIGNNSRPENIKWIRSGSRRQYYADPAGIYQNHQLKILCEHYDYKP
                     AHGEICLFKTNHHSTIYRPEKVLLNASWHLAFPFIFEAEGNIWCLPESAQSNQLRLYR
                     WDTKKEELMFEKIIISDLQAVDPIIFHHNRKWWLMFTDKTKSNYSLHLWYAEDFRGPY
                     FPHINNPVKTDIRSSRPAGNIFYMDGRLMRPAQDCSVISGHRITLNEIIEITPFSFVE
                     TTRFFVEPPKSRQHFKGMHTLNTAGPYVIFDVKQDLFIWDNFKRRLLRKTKKIIVQ"
     gene            complement(13230..13472)
                     /locus_tag="PLF20_00575"
     CDS             complement(13230..13472)
                     /locus_tag="PLF20_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79538.1"
                     /translation="MITHPAKFFQEVNRVLKPQGYFMMSSDYLYLSWSVEDYMRHSRL
                     HLVKLAMENGYTVYFIESFGGILTSIYSMVIRYMRI"
     gene            complement(13469..13723)
                     /locus_tag="PLF20_00580"
     CDS             complement(13469..13723)
                     /locus_tag="PLF20_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79539.1"
                     /translation="MLHNKIHRYQNLFSGNILDIGARSKPYRNLFTEVAQYVGTNTCR
                     HHQINHLRPDEDTTDVWIGDGTALPFQNATFDGVVFKSSL"
     gene            complement(13830..14723)
                     /gene="folD"
                     /locus_tag="PLF20_00585"
     CDS             complement(13830..14723)
                     /gene="folD"
                     /locus_tag="PLF20_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOX79540.1"
                     /translation="MKLLDGKTLFANIKGEIKTEVSQIIDRNERPPHLAAVLVGDDPA
                     SLSYVASKEKACRQVGITSSLYHYPENFTENELLQVIDFLNHDQDIDGFIVQLPLPPQ
                     IDPNKIILSISPDKDVDGFHPYNLGRMMMGQPNFIPATPLGIMQMLERHQIETKGKHC
                     VVLGRSMIVGTPVSILLSRKAKPGNATVTLCHSYTQNLKEICLTADILIIAIGKPRFI
                     TADMVKEGSVVIDVGIHRIPDNTKEKGYMIVGDVDFEEVSKKAEWITPVPGGVGNMTI
                     AGLLMNTLNAWKQRNIRQNIN"
     gene            complement(14803..16140)
                     /gene="ffh"
                     /locus_tag="PLF20_00590"
     CDS             complement(14803..16140)
                     /gene="ffh"
                     /locus_tag="PLF20_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOX79541.1"
                     /translation="MFEGLTEKLDRAFKVLRGQGSITEINVADTVKEIRKALLDADVS
                     YKIAKQFTDEVKQKALGQDVLKAISPSQLMIKIVHDELVTLMGGQKSDLNLKGNPAVV
                     LMAGLQGSGKTTFSAKLAHYVKTKYGRSPLLVACDIYRPAAIEQLNVLGQQIDVEVYT
                     EENNSDPVKIARNAVNYARDKSGHQVVIIDTAGRLAIDQLMMDEIAAIKKAVNPQETL
                     FVVDAMTGQDAVNTAKAFNEVIDYDGVVLTKLDGDTRGGAALTVRAVVDKPIKFVGLG
                     EKMDAIDVFYPERMADRILGMGDIVSLVEKAQEQFDAEEAKKLQRKIAKDEFNFDDFL
                     KQIHQIKKMGNVKDLMGMIPGMGKMVKDLDVDDDAFKSIEAIIHSMTPEERQNPAIIN
                     GSRRKRIAMGSGTDVTEVNRLMKQFDETRKMMKMVTTGGPRQMLKNMKQQKKR"
     gene            complement(16180..17070)
                     /gene="fabD"
                     /locus_tag="PLF20_00595"
     CDS             complement(16180..17070)
                     /gene="fabD"
                     /locus_tag="PLF20_00595"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOX79542.1"
                     /translation="MKAYLFPGQGAQFSGMGKDLYEKSELARELFEKANSQLGFRITD
                     IMFQGSEDELMQTKVTQPAIFLHSVILARVLGEEFKPDMVAGHSLGEFSALVANKVLT
                     FEDGLKLVYARAMAMQKACEMNPSAMAAILGLDDSNVERICRSISDEIVVPANYNSPG
                     QIVISGSIVGVSMACEKLKEAGAKRTLSLKVGGAFHSPLMEPARVELAEAISATRFVK
                     GICPIYQNVTGQPVTDPEIIKANLIKQLTAPVRWRQTIENMIADGAKIFIEVGPGTVL
                     QGLVKKIDKEAEAMSVQWEV"
     gene            17274..17840
                     /gene="frr"
                     /locus_tag="PLF20_00600"
     CDS             17274..17840
                     /gene="frr"
                     /locus_tag="PLF20_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOX79543.1"
                     /translation="MLSEESEFCLEETRESMDNALLHFEKEFQKIRAGKATPQMLDGV
                     KVDYYGAITPIDKISNITTPDPRQIIVQPWDKTMLALIEKAILAANLGFNPQNNGEVL
                     RIIVPPLTEERRRELVKKAKVEAENAKVTIRNIRRNAMETAKKLKKDGIPEDEIDQLE
                     EEIQKITDAYIEKIDKAFEIKEKDIMTV"
     gene            complement(17883..18896)
                     /locus_tag="PLF20_00605"
     CDS             complement(17883..18896)
                     /locus_tag="PLF20_00605"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOX79544.1"
                     /translation="MMNLMRIFSWIRLIRCLTFRRSWNFLKNYASYSISQWFDRYIHW
                     GFPWTLSIEASGICNLHCPECPVGSHSLTRSAGLLRLHDYAQWLDESSPELFYLILYF
                     QGEPLLNEDFAAMVKMAHQKRIYTITSTNAHFLTSELSENIIMAGLDEIVISLDGTDS
                     LTYEKYRKGGSFDKATEGIRQLVAARKRSNRKNPFIRLQFIVMKHNQHQINKIQSLGK
                     QLGVDAVELKSAQIYSPGDENGLLTDIPEFARYIKGEDSLLLMKNRRKRGCRRMWQGS
                     VITWDGRLIPCCYDKDAVHSYGLLDSSTQVKTIWTSPNATIFRQNVMKSRDVYEICRN
                     CGE"
     gene            complement(18896..19990)
                     /locus_tag="PLF20_00610"
     CDS             complement(18896..19990)
                     /locus_tag="PLF20_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOX79545.1"
                     /translation="MASHSRRWMLVLTLLIIVVLIYYFTFVFWYIGIAGVFTLLGRPI
                     ERKLRGAGFKRFRLPAWASSLITLLILILVVVILLYGFVAIVYQQALVITKIDFNTLA
                     VNLAPLLEDIENFLHQYHLLPSGENLEQAIVQRLGDIFEFSSIYKLLGSLTGFVSSFA
                     VGAFAVAFLTFFFLRDDKLLMQLFLLFVPYEYETRAISVLNTSRDLLGRYVGGLFLEV
                     FIMMVLLSVGMMIAGIPNALLIGFIGGFFNLIPYLGPFIGGALGVLLTLFWVLAEGSY
                     ATFWVQPLIVVGVFIAVNMVDNFVLQPQIYSKRVNAHPIEIFLIMMMGGSVGGIIGMI
                     LAVPAYTLIRIVGKEFLSSFDLMDKLTRNL"
     gene            complement(20117..20629)
                     /gene="tpx"
                     /locus_tag="PLF20_00615"
     CDS             complement(20117..20629)
                     /gene="tpx"
                     /locus_tag="PLF20_00615"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HOX79546.1"
                     /translation="MKKNKGITTFMGKPVTLLGDMVQPGEIAPNFTALAADSKPVNLS
                     DFKGKVIIISSVPSVDTGVCAAQTRRFNTEAASLGEVVILTISCDLPFALGRFCAAEG
                     IDKVITLSDHKDTDFGLKYGFLIEELRLLNRGIVVIDREGIIRYIEYVKENTHHPDYD
                     AAIAAARKLI"
     gene            20884..22791
                     /gene="htpG"
                     /locus_tag="PLF20_00620"
     CDS             20884..22791
                     /gene="htpG"
                     /locus_tag="PLF20_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOX79547.1"
                     /translation="MATQQKGKINVQTENIFPIIKKFLYSEHEIFLRELISNAVDATT
                     KLKALASIGEFKGELGDLRIEVKVDKKKKTLTVSDKGIGMTAEEIDKYINQIAFSSAE
                     EFISRFKDKNDTEVSQLIGHFGLGFYSAFMVADKVRIESLSWTEGAEAVVWECDGSPE
                     YAMKPSNKKTRGTDVTLYINKDNEEFLDEYRVLELLKKYCRFLPIEIVFGTEKVREKV
                     EGEKDKDGNDVYKEVEKPRIINNTKPAWTKKPSELTDEDYKNFYRELYPMNFEEPLFW
                     IHLNVDYPFKLTGILYFPKIKERIEVQKEKIQLYCNQVFVTDSVEGIVPEFLTLLHGV
                     IDSPDIPLNVSRSYLQSDPQVKKISNHIMKKVADKLEEIFKNNREEFEKKWDDIKVFI
                     QYGMITEPKFYERAEKFYLFKDIDHKYYTFEEYKALIKPLQTDKEKNLVYIYSNNPET
                     EYVFISAAKEKGYNILLMDGILDNHFINHLEMKFDKCRFVRVDSDVIEKLIPKEEESI
                     SKLTEEQKENLKKLFEEVIDKNNFTLVLENLSESDLPVFITKPEYMRRMKDMSALGGG
                     MSYMDALPDQFNLVINTNHPAILHILDENDEEKRKNLAHQLKDLAMLQQGLLKGEALT
                     DFVKRSVEFIR"
     gene            22935..23525
                     /locus_tag="PLF20_00625"
     CDS             22935..23525
                     /locus_tag="PLF20_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007757729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOX79548.1"
                     /translation="MKKGLIIGLSIAIIVLIFILTGVSMYNSLVKAEENVNAQWANVE
                     NVYQRRADLIPNLVNTVKGYANFEQKTITDVIEARAKATSVNINPEKLNEASLQEFQN
                     AQEQLSSALSRLMVVVERYPELKANQNFLELQSQLEGTENRIAVERRKFNEMAQAYNK
                     SIRTFPRNIIANMFRFERKAYFEATEGAEQVPKVEF"
     gene            23529..23996
                     /locus_tag="PLF20_00630"
     CDS             23529..23996
                     /locus_tag="PLF20_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOX79549.1"
                     /translation="MALLKIKSVRDYFTPEERERLTEAVKEMELKTSAEIRIHIERKC
                     QGDPLDRAAYIFGKLGMHKTALRNGVLIYLAIENHQFAFIGDRGINAVVPPNFWTDLK
                     LKAQKYFSEGQFTQGLLEVIEEIGTYLQKFFPYKENDINELPDEISFKDKPSK"
     gene            24010..24828
                     /locus_tag="PLF20_00635"
     CDS             24010..24828
                     /locus_tag="PLF20_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOX79550.1"
                     /translation="MQDNSLSKGLRTLAFLLFTSLFFITSLKADDLPPRPFPPRLVND
                     LAGIIDPYAIIQLEQKLVAFNDTTSTQIAVVTVPSLCGYDIADFTDRLAEKWGVGQKG
                     KDNGVMILVKPKNNEGNGYVRISVGYGLEDVITDAVARRIIEKEMIPYFRQGDYTKGI
                     MAGTEVIMNLSSGKFKAEDYIGGDPNAAIGIAIFIIILGGIFLMRFLFSPFAIGRSSS
                     FWPMIVLMNMFGRGGHKGKWDNFSSGSGWFGGGGSSFGGFGGGSFGGGGASGSW"
     gene            25386..25517
                     /locus_tag="PLF20_00640"
     CDS             25386..25517
                     /locus_tag="PLF20_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79551.1"
                     /translation="MNKLPGNILLGVKAGIFFSGSQLQINELKPTPLLPQLGYPVIL"
     gene            25981..26154
                     /locus_tag="PLF20_00645"
     CDS             25981..26154
                     /locus_tag="PLF20_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79552.1"
                     /translation="MVAAGRNGGFLCIPLELAYLSEQLEQLLFLDDHKLCLFAYTLQD
                     WIHLLGIEIYKEK"
     gene            26443..30081
                     /locus_tag="PLF20_00650"
     CDS             26443..30081
                     /locus_tag="PLF20_00650"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX79553.1"
                     /translation="MKNLRFFIFLLAISFIPVILRAQTEESDTASFPYWIEMMQDENI
                     NFYQVVKAFDTYWEGRPVTKGCGYKPFKRWEYSMRNGRIYPDGTRKPASYTWNAYFDY
                     IKSYRDSVYGHWESIGPKTYPSLGYKGLGRINAIAFHPTNPDIFYIGAPSGGLWHTYD
                     GGNTWGSNTDNLPTLGVSAIIVDYSNPNLIYIGSGDRDANDAVGMGVLRSNDSGLTWE
                     LWNNGMGEKIVGRLIQHPTNPSIILAATSGGIFKTIDGGANWAIKKTGNFKDIVFKPN
                     NADIIYACSGANFFRSTDGGENFTQITNGLTGGQRAVVAVTPANPEYVYLLVSDNSSG
                     FKGLYRSTDGGTSFVTMSNSPNILDWSCDGSGSGGQAWYDLEIAADPMNPNLIYAGGI
                     NIWKSYNGGETWQITAHWYGGCNVQAVHADHHVFEFSPIHHHLYVGNDGGIYSTANQG
                     QTWTEYTSGLVISQIYKIGQDVADTSHLIIGMQDNGTSTLINNVWADTRGGDGMECII
                     DYQEPAYSYASLYYGDIARFYNNNGGYTIAAKGKFGITEDGDWITPYTLHVTDPNRMF
                     IGYKNIWRGQYIRTTPQWVKISDNLAGNNNTNIRIVEQSQANPDILYFARWDNKLFRT
                     DNANAGQPVWVNLTSLLPLSGQTPSALRPHPTKPDIIYMALGGKIYKSQNRGQSWTDI
                     SGSLPNVEYSSIAFYIRAHEGLYVASDIGVFYKDSTLTDWIQFSNGLPVDASIREIEI
                     AYNNQNNEYDRIRAGTYGRGVWSSSVYRTPPTASFTSDVIIVPVTGQVHFKDISSGIP
                     SSWQWTFEGGQPATSTIQNPVITYNNPGSFKVKLIVSNSLGIDSIEFQNYITVSNTIL
                     PLVGFYADNTIPCSGQIVRLYDTTLYGPISWHWQFQPNTIAYHNGTFYNSQNPVVQFN
                     ENTEYTITLTASNPNGTSSVTRQNYIHSGGYLMPFYEDFETLNFADRSWTVENPDMGI
                     TWDMAQVESIHGPTVAARMQFFYYYLMNQRDRLISPPLNFSNENKVILSFEHAYAQRY
                     NQRDSLIVLASDDCGSTWTRIFASGPDGNGIFETAPKTESEFIPSTAEDWCGSGWGAN
                     CIQLDLSAWAGKPNCRIAFEAFNKLGNNLYIDNIVVSRSGVGMAHSVENTFIVSPNPS
                     NGIFNITTPYHNQQRAEIYSTDGRMLFSKDFSNNNLKINVSSYPSGIYTLKIIANQQT
                     YQIKLIKK"
     gene            30306..33086
                     /locus_tag="PLF20_00655"
     CDS             30306..33086
                     /locus_tag="PLF20_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOX79554.1"
                     /translation="MKKLQFFILTVSIFVGLLPYRSIAQGWRSNEMEINVLVKTLEDM
                     ELIHRSGWSYDRPSSIPGIARLYLIPKELEELKQMGMEYSISIPDIAEHYRDLWGGVV
                     PPGYYTYEQLIAIEDSLAAAFPAICKKIVIGTSVQNRQLAVLKISDNVEQDEAEPEIF
                     FEAGIHGDEVGGPENLIRFARDLCKGYGSNPQITNLVNTRELFILVMTNPDGRVQMSR
                     YNANLVDINRDGGYMWDGEGNSPGAFSQTETKAIRDFAAQNQFVVFTDYHSGTEFISY
                     PWSYRPQMAPDQPHLHQLAHVYATNSGYTNIPYASGYSGMYPINGSTKDFNYGLQGSI
                     SWSIEISNNKQPPASQIYYYYLKNKGAMIAISEQCGKGIYGVVTDSVTGNPIPAKIRI
                     GGTVPLYNDPVVGDYHKFVVPGIYTVEASANGYKTKTITDVQVLPDSSVGVDIALSPK
                     QGHYAFQIKACRIPGNNFYDEGHTWAALGEQDGINYSLGKNGWIILDMYDTIYNGNGN
                     DILVVEGDSSPESYSVYAAITQDGPWKLMGSASGTHEFDLTSANLNKARYLKIVDGGT
                     GSYFGEDAGFDLDGVKVLYIPVSANFTASNTMPCIDNSIDFADNSLGNPTIWQWLFQG
                     GNPSSSNSQNPSGIMYPNPGIYDVTLTVGDGVTSSSLTKSGYIHVYGTPLPPLTPLGD
                     SSFCQGLSQSDYTTAGAPNATSYEWVLTPTDAGIITGNWILATVNWNPNFNGQAMVKA
                     RETSPCGTGQWSLPKVVDVMERPIVDLGNDTIIAASASLLLDAGNPGCSYLWNTNETT
                     QTIIADTNGIGAGIHEYWVRVTSPNLCSSSDTILINFSIDIGMPDFSVSSIKVFPNPT
                     TQGLWIELNEEIGMSDIFFIDTKGEKWKLPVSIFHKDTLFYISTKTLPSGIYFLRIEA
                     SAKALTIPIIKR"
     gene            34072..34656
                     /locus_tag="PLF20_00660"
     CDS             34072..34656
                     /locus_tag="PLF20_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOX79555.1"
                     /translation="MPKACFYLNNSLANSINEHVDISVARCYQCGKCSAGCPIVEFMD
                     YSPSLVLRYLQTELPENDDAVLRSKTIWYCLSCEMCIARCPMEVDIPVIMDFLRIESI
                     RLRKTHKEVKNIIRFHKAFLNTIKRNGRLHEMGLVLDYKTRSLDLLQDLALSPGMFKR
                     GKLRILPEQVKNRKEIKHIFTIGSNKRKNKSIKN"
     gene            34660..35565
                     /locus_tag="PLF20_00665"
     CDS             34660..35565
                     /locus_tag="PLF20_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HOX79556.1"
                     /translation="MKLGFYPGCSLEGSSREYNESTIAIAEKFDIELVQIPDWNCCGA
                     TAAHNLDKKLSLLLPARNLVLAEEGGLKDVVVPCAACFSRLSTTHHELLLDEELRHQV
                     DNIFGKHYKGSIRILNVIQFIHEYILEKLDEKIVNEFRNKVACYYGCLLVRPHKILNF
                     DRTEDPQTMDLVMKKLGAEPIDWAYKTECCGAGFSVSFTDAVSKLSGKIVEDAIHRGA
                     EAIIVACPMCQSNLDMRRCGIDKYLGKRTNIPVLYITQAIGLALGMNMKQLGLHRHFV
                     PVNFDKFKLIKKQTKVEEPQKMEEK"
     gene            35565..37568
                     /locus_tag="PLF20_00670"
     CDS             35565..37568
                     /locus_tag="PLF20_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOX79557.1"
                     /translation="MARIGVFICHCGENIGETVDCTKLAEVTSRFPGVVKSVDYKYMC
                     SEPGQTLIKEAIKENRLTGVVVAACSPRMHESTFRKTCSEAGLNPFLCEIANLREHCS
                     WVHEKSEEATNKAIDIVRALVEKVKLNEPLYPIKVPVTKTALVIGGGVAGIQAALDIA
                     NGGHKVILVEKNPSIGGHMSQLSETFPTLDCSQCILTPRMVELAQHPNITLYSYAELE
                     SLDGFIGNFKVKIRKKAKSIDENLCTGCGLCTTKCPVKKIPNEFDENLSYRKAIYVPF
                     PQAVPNKPVIDRENCTYYLKGKCKICERFCPTQAIRFDQQDEIIEVEAGAIVLATGFD
                     IKRADFFPEYGFGKYKDVIDGLQFERIASASGPTLGEIRRLSDGKIPKKVVFIACAGS
                     RDPAKGIEYCSKICCMYTAKHAILYKHKIHDGQAYVFYMDIRAGGKNYDEFTRRAIEE
                     EGAKYIRGRVSRIYERDGKLIVVGADTLMNARPVVIEADMVVLATAGVASPGSEELAQ
                     KLHVAYDQYKFFTEAHPKLRPVETNTAGIFLAGACQSIKDIPESVSQASGAAAKVCGL
                     FSQNELTRDPLIAVVNRTAPPLYSTCVGCFLCQSVCPYQAIEKEEIKAKDGSVIKIIA
                     KINPGLCQGCGSCVALCRTKSIDLLGYTNRQIYAEVDAILNTL"
     gene            37570..38016
                     /locus_tag="PLF20_00675"
     CDS             37570..38016
                     /locus_tag="PLF20_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018462502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOX79558.1"
                     /translation="MDEFEPKIVAFVCNWCTYAGADLTGTSRIKYAPNVRIIRFPCTG
                     RIDFMFILKAFARGADGIIVSGCHPNDCHYTSGNFHARRRWIVFRSLLDFLGIDVRRI
                     TFSWVSAAEGAKWADLVNQTTAEIRKLGPYTSYHKISKPEEEVIYG"
     gene            38009..38809
                     /locus_tag="PLF20_00680"
     CDS             38009..38809
                     /locus_tag="PLF20_00680"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOX79559.1"
                     /translation="MDRVKQKARDLLDSNKVQVVIGYEEGTDGTARPVFIMDSKNIDK
                     LIFNDRCYHNLAVYLTKVEVEKIGRVAVEASLNTLKGIVKLASEGQFVFEDIDILGVN
                     RNGELYEFASLDEVIDYIDQHPVEYSPEEISLLRKIESMTREERWEYWQEEFSRCIRC
                     YACRAACPNCYCDACIVDINQPQWISPTPDALGNLEWHIIRAMHLAGRCVNCGACAKA
                     CPVDIPLNILNLKLAEEISKNFGISEEFLLTNYPLAIYDPNDKENFIK"
     gene            38814..39830
                     /locus_tag="PLF20_00685"
     CDS             38814..39830
                     /locus_tag="PLF20_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOX79560.1"
                     /translation="MTPQNKLLKKECLPTFVETLVKGGKLLYAPTKENERIEFKLIEN
                     FSEIYLDYIQTTKSIKSIVFPPIEKLFEYVADKEHVEIKDVNLNTIPETIVFGAHPCD
                     AASFKVLNAVFTWDCNDEFFLTRKDKTTVIGLSCKNHDDYCFCTSVDLNPGSTEGSDI
                     LLTRIENDNYLAEIMTEKGEAVYKLVKNLFEPAPQVDKNAFVTQLPKLFDLNDLRGKL
                     SFEGLSWMQQSLRCLSCGTCAYVCPTCSCFDIQDIGNQTKGKRVRIWDTCALPQFTLH
                     TSGHNPRHFQNERWRQRIMHKFSYEPERLHVYGCVGCGRCSRACPVNINILENLIEIS
                     GGNK"
     gene            39863..40669
                     /locus_tag="PLF20_00690"
     CDS             39863..40669
                     /locus_tag="PLF20_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HOX79561.1"
                     /translation="MRIEKVTDEAPDVKTFRLKFIDDVQARNFDFKAGQFGEYSVMGE
                     GESTFCIASPPTRKGYIECTFRKTGRVTSALANKEEGEIIGFRGPYGNRFPIEEWKGK
                     NLLFVAGGIALPPMRCVIWNCLDLREDYKDITIVYGAKTVDDLIYKNELEEWNERPDV
                     TLVTTVDPGGETPAWKGQIGFVPTILEKMELKAENTIAVVCGPPIMIKFTFPVLVKAE
                     FKEADIFTTLENRMKCGFGKCGRCNVGSVYVCKDGPVFTYAQLKKLPAEY"
     gene            40978..41133
                     /locus_tag="PLF20_00695"
     CDS             40978..41133
                     /locus_tag="PLF20_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79562.1"
                     /translation="MSVFHSLIPKLQPSLTIGHSISRLSQYFLDFLPLLYGSIVVPET
                     GLEPAHP"
     gene            complement(41099..41182)
                     /locus_tag="PLF20_00700"
     tRNA            complement(41099..41182)
                     /locus_tag="PLF20_00700"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41146..41148),aa:Leu,seq:cag)
     gene            complement(41209..41295)
                     /locus_tag="PLF20_00705"
     tRNA            complement(41209..41295)
                     /locus_tag="PLF20_00705"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(41259..41261),aa:Leu,seq:gag)
     gene            complement(41554..42294)
                     /gene="pepE"
                     /locus_tag="PLF20_00710"
     CDS             complement(41554..42294)
                     /gene="pepE"
                     /locus_tag="PLF20_00710"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HOX79563.1"
                     /translation="MKLLLISNSTNYGEEYLHWPKPFISDFLNKFRVKKVLFVPYAGI
                     SLSPLGLKESYDVYEKRVQEVFKSLGFEIYSIHRQSHPIRAVMEAEAIAIGGGNTFHL
                     VYCLQKRGLMSAIREQALHGIPYLGWSAGSNVACPSLMTTNDMPIIQPSDFECMGLVP
                     FQINPHYLDKNPEGHGGETREARIEEFIILHPRIPVVGLREASLLEVIDDKMVLKGSR
                     AMRLFQFGNLPCEFAPGSDVSQLLKPIN"
     gene            complement(42319..43341)
                     /locus_tag="PLF20_00715"
     CDS             complement(42319..43341)
                     /locus_tag="PLF20_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79564.1"
                     /translation="MKKLTPCRRICQRYIMLVLLIFGNDIYTIHGQSFGIPAGMGSPF
                     WNGFYTKDTTNCQNIRIAAFYSYANSAFEGKGNILSRQPFDELIRKGFFMNAKVPLYK
                     NFSLGLLLPWYESQVKKTVNSQEMRSYRNAEVSEIRLKAEYLYHFTETSVFLEISTGL
                     PLRETRTSYISNKPPIGNDGYWNLGIQAAFMHKLSLNSQMFIHNEFLFRFPRSGAIFE
                     GDTALILTNNRYNAIQATIYLHPYFTSQGGYVYKLNKLKIMVGYDYFYGWSDRAENIL
                     PEPDEKLNKIIFQAMGADAFIHSLCLGASYKSKNWQFDLSTRLAFFGKNALKENMLTF
                     RTTYIF"
     gene            complement(43406..46024)
                     /locus_tag="PLF20_00720"
     CDS             complement(43406..46024)
                     /locus_tag="PLF20_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79565.1"
                     /translation="MKTSPFICFILFLFLAQASFAGTQDTVWLKWKKDTSRNIQATNR
                     ILEELAFENGLSYRKIKLGYVLYHQKQINDSSVGKWKVVLHHITGPTKYLGYDLSDFL
                     LPLYLQAYLQHSFHGIIYTQEVKLEHDTVILEFSPKVNLSTDSLSLHFRNANLDEEIE
                     RQLKRVMDEIKTYRGLCASSLYFENVLRQGYNPHDPRISDILAELIEGIRVKESLDSI
                     FSALILPLEKTDPCNLYNSLKAIDIEIYRHRLAFERLLDTDPLSPNDLTPLSDRIFGF
                     WISWIRHEEFSNPLNNGVIAEVARLDPHGKWIEMAHELLHSALLKMGYTNEDEFLKNL
                     SRQLTKKMLQEGEALNKQTLFIQANIILNNALKIAQAADLEDETQAVESQIKVSVRGL
                     YNTYLKVARDGILNGNIVMGEFYLKKALEYQSENREILLGSQPSKSVYEIFSDVCLDK
                     AIILNKEGKFEQALPFLEKAMEYAGRQSIYLRQQEIKQTLSKTLQEIYRQKVISALSS
                     MQLGNYIEGKRLLDEALTMKKTNATSIESIPDEDSAFHLYQKASLYVVMDSLIQISNE
                     LPLDSLLNATSIILLRYAENLADDSLICKKKLELGKLQFERMMAAVGSLLWDFKIEEA
                     HQLWLNANKMIPQWNLQDCSEINERLNEMAERIEIQECRFARYRLELFEYKVRSAFQE
                     GDYLMGDSIADSALQIKTSVADCPLDDSGIAGLLHQYHSLIEYNRLLEQLTTTYETLL
                     ADSLEKLRLKLYSLISSDSSISREFPLPDTIWLVELHPSEKIINYYFENLKEENRWSD
                     ILNLIEKFRQSGLSADWFETWQENLGHHLARIDKIPAKKEASLEYLDQMLPDRVWYKT
                     LRKAYLKEKLGWRAIF"
     gene            complement(46041..>47169)
                     /locus_tag="PLF20_00725"
     CDS             complement(46041..>47169)
                     /locus_tag="PLF20_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79566.1"
                     /translation="GSLVNGSPHPIKLNLTGWGPYYRLRNKDTLFIAGNAWFGIKTHD
                     LLNDEPNHNGVKSVELFIDSIPVFAIEFNRLYFDKGRYINALCDYSEIVLNKQWIIQT
                     RKAPYSRLQNIYRILLNNGSYAFLPDNIYHLKYVVSDHYGNESILRFVVKGIPKVYAP
                     IRPFVPSHIQFSGDREASYDTVDFCFWAPIGAFYDTVNFSYKRITSGSAFFSDFHRIH
                     TPTTPIHSSAKISIRPNRLPSRLHHKAIIVKVNQGNKPSAMISKWEGSSLKASIRSFG
                     TYAVLADTLPPVIKNLNFKNGQNIKNLPRLNVRISDDLSGIDSYIGSINGEWILLEYD
                     AKNNLLTYFPDEMLSTGENLLHIRVTDNVGNFSEATYNLVY"
BASE COUNT        14130 a         9479 c         9494 g        14066 t
ORIGIN      
        1 cggtttcggc agaagtaaca aacgtaatat ccatgcccat aatcttattg actttatcaa
       61 tatttatttc gggaaagatg atttgttcag aaatgccgaa gctaaaattt cctctcccat
      121 cgaagccttt atcgctaatg ccgtgaaaat cacgaatacg tggaatggca atggagatca
      181 agcgatccag gaactcatac atacgagcgc ctctaagggt tactttcact ccaacgggca
      241 tattgcgacg cagcttgaag ttggagatat cttttttcga tttggtggct atagctcttt
      301 ggccagctat tagggtcatc tcttcgatgc caacgtcgat cagcttttta tcggctatag
      361 cgtcacctaa accttggttc agtgaaatct tgaccaagcg tggtacctgc ataacagact
      421 tataattaaa ctgtttcact aatgcaggga taatttcttc ccgatatttt ttttgtaaac
      481 gtggtatata gtccattact tgattacctc ccctgttttt atagcatatc taactttttt
      541 attggttttg gggtcaattt tgtagccgat gcgtgtaggt ttacctcctg gcccgatgag
      601 cattaggttg gatacatgaa cgggagcttc ccgttttaga atgccacctt taggggtttt
      661 tgcattgggt ttggagtgtt ttaaaacgag attcactcct tcaacgatgg ctttctgctt
      721 ttcgacgtgt acctcgagga ctctaccctg acggccttta tcttcgcctg taattaccat
      781 caccacatcg ccttttttta tgtgaagttt cggcttcata gtttatttga tttcaatatt
      841 ataacacttc aggtgcaaga gatacgattt tcatgtattg tttttcacgt aattcccggg
      901 ctaccgggcc aaaaatacgc gtaccagcca tttcaccggt attgttcaga ataacacagg
      961 cattgtcgtc gaaacggata tatgatccat cggcccgcct gatttctttt ttcgttctca
     1021 caacaacagc cttggttact gtaccttttt tgatggtacc cgaaggaagg gcttttttga
     1081 cggtaaccac gattttatcg cctaccgtag catatcggtg ccctgtaccg cccaaaatac
     1141 ggatacatag cacttccttg gctccactgt tatcggcaac attcaaacga gtttcctgtt
     1201 gtatcatagt tattttgctc tttcaataat ttcaactagt ctccagcatt tttctctgct
     1261 aagtgggcgg gtttccatta tccgaaccac atcacccaca ttacagtcat tattttgatc
     1321 atgtaccttg aatttggtgc ttcgctttac aaattttcca tatttaggat gcatcaagcg
     1381 gcgctcgact ttcacaacaa tggttttgtc cattttatta cttactacca ctccaattcg
     1441 ttccttacgt aaattcctgg tttccattat ttaacttgtt tttttattct tttcgagttc
     1501 acgttttttc aactcagtaa gaatgcgagc aatcgttttc cgattttggc gaatcttcat
     1561 ggggttatcg agaggagaaa ctgtatggct gagtcgcaaa cgcgtcagtt ttttcctttc
     1621 ttcctcgagc ctttcaacaa ggtcgttggt ggagagttcc tttataactg cctgtttcat
     1681 ctttaaaaat tttatgttta caaagcttga ggtttattct tcaagataat cgggacgtat
     1741 aacaaatttc gttttgatgg gcaatttttg agctgccagt cgcattcctt cacgggcaat
     1801 ttccagtggg attccatcgg cttcgaaaag gattcttccc ggggttacag gagcaacata
     1861 atattctact gctcctttac ctttacccat acgtacttca gctggttttt tggtaatagg
     1921 tttgtcgggg aaaatacgta tccagatttg tccctcacgt ttcatgtgac gtgtgagggc
     1981 ttggcgggca gcctcgattt gtcgtccggt aatgaaggtt tgttcaagtg ctttgaggcc
     2041 aaatgatcca aaagcaattt cataccctct ctgggcattg cctttcatcc ggcctttttg
     2101 catttttctg tatttagttc tttttggctg taacattgta ttacaggttt taatttttta
     2161 ctgatagatt atttgcgttt acctctggat cgggggcggc tagttccggg aacaggcgat
     2221 cgttgttgac gggtagccga cgatgtggca aaaaaatcag gccttccata aatttcaccc
     2281 cggcaaatcc atacttttat gccaatgcgt ccataagttg tatgggcttc gtctctggca
     2341 taatcaatat cggctctaag ggtatgcagt ggaattctgc cttccttgaa catttcacga
     2401 cgtgcaattt cagcaccacc taaacgaccc gatatttgaa tcttgatgcc ttcagcccca
     2461 attctcatgg tattggaaat tgccattttg atagccctgc ggtaagatat acgccgctca
     2521 aggtcacgtg ctacattatt ggccaccaga taggcatcca gatccggacg ctttatttca
     2581 cttatattca attgaatctc tttgccggta accttgtgca attcttcctt caacttatct
     2641 acttcctgtc caccttttcc tatgataatt cctggcctgg ctgtatggat agtaactgtg
     2701 ataagtttca aatttctttc gatcatgatt ttagaaactc cagctttaga aagcctcgca
     2761 ttgaggtact tccttatctt gaaatcctct atgagttttt ggtcaaactg acgacctccg
     2821 taccagtagg agtcccatcc tttgataatg ccaagcctaa ggcttattgg attagttttt
     2881 tgtcccattc aatgaatgaa tttatagtta ttgctttaat cggttatttc ttcgatttcg
     2941 tttttattcg atagctgatc gcccaaaacg agagtgatgt gattggagcg tttacgaatt
     3001 ctgtagccac ggccgcgagg tgctgggcgt aagcgtttca atatacggcc tccgtccacg
     3061 gtaatagttt gaatatataa ctgtgcatct tccatgcgca taccttcatg tttttgctgc
     3121 caatttgcga tagccgattt cagaagggta tatatttggc tggctccttc ttgcttcatc
     3181 gtatgcaaaa cgatgagagc cttttctgct tgcatgccac gcacaagatc agctaccagc
     3241 ctcatctttc ttggagaacc agagactttg gcaagcctgg ctgaggtttt taatttgttc
     3301 tctgcttttc ttaattcagc ggatctacgt tttcttgctc ccatggttta ttattttttt
     3361 gctttactac ctgcatggcc acggaatgta cgcgttggcg caaattctcc taacttatga
     3421 cctaccatat tttcagtaat atataccgga atgaatttat ttccattatg cacagcaatc
     3481 gtttgaccca caaaatcagg agaaatcatc gaagatctcg accaggtctt tatgacattc
     3541 tttttcttgc tttcaacctg atgcagaact tttttctcga gcttgtagtg aatataagga
     3601 ccttttttca gtgaacggct cataattaaa ctattttgaa actattattt ctttctgcgt
     3661 tcgataatat atttagaaga agctttctta ggtttacgtg ttctgtagcc tttagccgga
     3721 attcccttac gcgagcgtgg gtgtccaccc gaagcgcgac cttctccgcc tcccatggga
     3781 tgatcaacgg gattcatagc aacaccgcgt gtacgaggcc ggcggcctaa ccagcgtgac
     3841 cgtcctgctt tccccgaaac ttcgagacta tgatcggggt tggataccat gccaatggta
     3901 gctttgcagg attgcaatat aaggcgagtc tctccagagg gtaatttgat aacggcatat
     3961 ttcccgtcgc gcgacatcaa ttgggcatag ctacctgcac tgcgagccat tttggctccc
     4021 tgtccaggac gcaattctat attatgtata atggtaccta atggtatttc acttaaatac
     4081 aatgtattgc cgatatcggg agatatacct ttcccactca tgatggattg accgacttta
     4141 agcccgttgg gagcaacaat ataacgcttt tctccatcct gatattcaac cagggctatg
     4201 cgagcagaac gattaggatc gtactctatg ctctgaacaa tagcagggac aagttcctta
     4261 tcgcgcttaa aatcgataat acgatacatg cgtttatgac caccaccaag atagcgcatg
     4321 gtcatcttcc ctgtattgtt cctaccaccc gattttttca cgggagtcaa taaactcttt
     4381 tcagggtttt gggtggtaat ctcgtcgaaa gcacttatta ctttaaagcg ctgactcgag
     4441 gtgatgggtt tgaatttttt taaggccatt tgtcttaaat gttgctgtaa aaatcgattt
     4501 tctcgccttc ggcaagagta attatagctt ttttataagc tctggtacga ccggcaataa
     4561 ttccgctctt ggtatagcgg tatttgtttt tgcctgtaaa gatcatggta ttgacttcca
     4621 aaatatctac gccatagagt tctttaatgg cttttttgat ttgaatctta ttggcttcgc
     4681 gcctcacaat aaatccatag cgattgagtt tctcgccttg gatgctcatt ttctcggtaa
     4741 tcagtggttt tactataatg ctactcattg aggcagattt tttgtgttaa ttttctgatt
     4801 ctgcataaag gttttccaga acaggtaatg agctttcgaa aaagatcatc cggttagctt
     4861 tgaggatatc aacggtgttc aaatcaactg cgcgcatgat ttttgcatag gggatgttgc
     4921 gtgaggcaag cagcagattc tgatcgggtt cattcacaac aagaagactt ctttgatcgt
     4981 ttagtttgaa atttttcaga aactcggcat aagtcttcgt tttaggagca tcgaaagtaa
     5041 aatcctcgac tataataatt ctattttcaa gggctttata ggataatgcc gacaagcggg
     5101 ccaaacgttt cgtcttttta ttcattttga aatgataatc cctcggacga ggaccaaata
     5161 cacggccacc gccgacaaac agaggattat tgatatcgcc ggcgcgtgca ccgccagtac
     5221 ctttttgtat ttttaattta cgtgtactgc ccgacatttc agacctttcc ttagttttat
     5281 gtgttccctg ccgctgattg gcctgtatgt tcttaacatc gagccatatt acatgatcgt
     5341 tgggttgaat attgaaaata gattcgttga gaatcacttt ccgcccaagg tcttctcctt
     5401 tataattata tactgttagc tccatctttc aagttttaaa tatgaaccat tagcacctgg
     5461 cactgagcct ttcacaacga taatattatt ctcggggatg atacgtaaaa tctttagatt
     5521 aatcattttc acctttttgt ttcccatgtg cccggccata cgcattccgg gaaatacacg
     5581 agaagggtaa gaagatgctc ccaatgaacc aggggcgcgt aaacggttat gctgtccatg
     5641 cgttgcatca cccaccccat gaaaattatg tcggcgtaca acaccctgaa atcctttgcc
     5701 tttgcttaat ccgcttacat cgatgaattc gccgaccgtg aaaacttcta ctcctatcac
     5761 ttctccaaag gattttcgat ggcctgcctc gaaacgtgta aattcggcca tgatacgctt
     5821 gggagtaata cccgccttgg caaaatgtcc tctcattggt ttgtttactt tacgctcatc
     5881 caaatcatca aaagccaatt gtatggcatc atatccatcc cgttctttgg taagaatttg
     5941 tgttaccaca caaggacctg cctgaattac agtacaagcc atattctttc ccgattcatc
     6001 atatatactg gtcatgccaa tctttttccc taataatcct gacattgctc gttaatttta
     6061 aggtacttat tgtttcatca aactttgatt tcgacttcta ctccactggg caattcaagt
     6121 ttcatcagtg catcgatggt tttcgaagtc gaactgtaaa tatccagcaa tcgcttatag
     6181 gagcataatt gaaattgttc acgcgatttc ttgtttacga atgtagaacg gtttacagta
     6241 aaaatcttcc gatgcgtagg cagagggatt ggtccgctca ctactgcacc tgtttgtttt
     6301 actgttttca caatcttttc ggctgattta tctaccagat tgtaatcgta cgatttcaac
     6361 ttaattctaa tcctttggtt cacgtttata gagaattaaa aatcattttc attgtcttat
     6421 aatatagcct tttattttta atatgacctg ttcctgaatt tcagggggac aagttgcata
     6481 atgcgaaaat tccatgctcg acgtagctct tccactcgaa atcgacctca atacagtaac
     6541 ataaccgaac atttcggaaa gaggtacaag tgcatccaca atctgataac taattcttgc
     6601 atcaattttc aataattgtc ctcttcttcg gtttaaatcg ctggtaatat cgccaacgta
     6661 ttcatcagga gttacgactt cgagtttcat aataggttcg agaagaatac attctgcttt
     6721 gcgaattgct tccctaaatc cactgatggc agcaatttcg aacgaaaggg cgtcgctatc
     6781 cactggatga gcttggccat ctatcaaacg aactttcatg ctttcgatgg gaaatcccaa
     6841 taaaggtcca ttcgacatgg cagaacgaaa tcccttttcg atggcaggaa tatattcttt
     6901 cgggaggttt tgcccatgga tttcgtttat gaattgtaaa cctttgacgc ctatgtcggc
     6961 tggtccaatt tcaaattcaa tatcggcaaa acggcctcgg ccaccggttt gttttttata
     7021 gatttcgtga tggcgaacat tacgagtaag tgcttctttg tatgccacct gaggtttgcc
     7081 atgattgcat tccacattat actcgcggtg tagcctatcg agaataatat cgagatgcag
     7141 ttcacccatt ccactaatga gtgtttggcc ggtttcttca tcgataccta cgaaaaatgt
     7201 ggggtcttct tcactgattt tttgcagggc attcatcatc ttttcgagct cgtcctgtgt
     7261 atgtggttct actgccatac tgatgacagg ttcaggaaaa gtaatggatt cgagcagtat
     7321 aggatgtttt tcatcggcaa gggtgtcgcc tgtccgaatt tgtttaaaac ctgctgcggc
     7381 tccaatatcg ccagcttcaa ttttctcgag aggattttgt ttattggcgt gcatctgaaa
     7441 aatgcgtgca atgcgttctc ttttcccagt agcaacattc agcaccacat ttccactttt
     7501 cagacttccg ctataaaccc tgaaaaatac gatacggcca acatacggat cggaggcaat
     7561 cttgaaagct aaggcagcga atggttcatc gggatcggga tgccggcttt cttcttcgcc
     7621 ggtaagcgga ttgatgcctt ttatggctgg aatgtcgagt ggcgaaggca gataagcagc
     7681 cacagcatcc agaaggaatt gaatgccttt gttatgtaat gacgaaccgc aaagcacggg
     7741 ggtgattctc atctcgatgg tagctttacg caaggcgttt ttaatttctt cttcggtaat
     7801 cgaatgagga tctgaaagaa atttctcgaa tagttgatca ctttcttcgg ccgtaccttc
     7861 tatcaattcg ttatgatatt tttcgaccaa cggaaagagt tgttctggta caggaacttc
     7921 gtagaaattg gtacccagcg aggtatcgtt ccattcataa gctttacatg aaatcaggtc
     7981 gattacgcct ctataatctt cttcttcgcc cagggggagc tggataggga tgggtttagc
     8041 acccaatttg gatttgatct gcgaaatcac ttcaaaaaaa tcagccccgc tcctatccat
     8101 cttatttacg aatgaaatgc gtggcactcc atatttatta gcttgtttcc aaacggtttc
     8161 cgattgaggt tcaacacctc ccactccaca aaaaatagca atagcgccat cgagtatgcg
     8221 aagcgatctt tcaacttcca ccgtaaaatc gacatgacca ggggtgtcga taatattaat
     8281 cttatattgt tcctgattat aatgccaaaa aacagtagtt gctgctgatg aaatagtaat
     8341 ccctctttcc tgttcctgaa tcatccaatc catggtggca gtgccttcat gcacttcacc
     8401 cattttataa ttcaccccgg tataatacaa tatccgctcg gtagtagtag tcttccccgc
     8461 gtcgatatga gccataatgc caatatttct tgtatagata agcctttcgg tcatgtatat
     8521 tgtattaatt tcgggtaaat ggtatttttc aggattttag ttaaaattta aaatgtgcaa
     8581 aagccttatt ggcttcggcc atacggtgaa tatcttcttt gcgtttgaag gcagcacctt
     8641 cttccttata agcagccata atttcgcctg ccagtttttc ggccattgat ttttcgtggc
     8701 gtttgaggga ataagagatc agattcttca tgccaaccga cattttgcgg gttggacgga
     8761 tttcggtagg aatctgaaaa gtagcacctc ctacacgacg gctacgtact tcgacagagg
     8821 gagtgacatt ggccaaagct tttttccaga cttcgaggcc atcctcaccc gttttctgac
     8881 tcacaatatc cactgcttcg taaaaaaggt cgaaagcaat acttttcttc cccgtgagca
     8941 tgatattatt tacaaacttg gtaaccattg tatcattgta cttggggtcg ggaagtatga
     9001 ttctttttct tggtttcgct ttcctcataa tctattataa tctattgcga attgtatttt
     9061 ctaaacaacg aatttacttc ttgccagctt ttgtatcttt aggacttgca gcaccaggct
     9121 tagttttagg ccgctttgca ccatatttcg aacgacgttg catacggcct tcaacacccg
     9181 cagtatctaa agcacctcga ataatatgat acctgactcc cggtagatcc tttactctgc
     9241 ctccgcgaat cattacaata gagtgctcct gcaaattatg accctcgcca ggaatgtaag
     9301 ccgtgacttc attaccattg gtcagtcgca cccttgctac cttacgcata gccgaattgg
     9361 gcttcttagg agttgtggta tacactcttg tacaaactcc tctgcgttga ggacatgaat
     9421 ccaaagctgg gcttttgctc ttgtactcaa gcttttgacg tcctctacga atcaactgtt
     9481 gtatagtggg catatcaact ttattagttc cttatctggt ttttaagcac cctctcaaaa
     9541 agggagtgca aaagtacaat taaatctttt acgaccaatg atcttcatta aaatttatct
     9601 caaaaaagat tggttaatcc tgaaaatgaa aagaaactgg ataaaattac cgatagcaaa
     9661 accggtaggg acagttacac gaaccttatg ccatctgttc aatcaattca cattctgaga
     9721 ggttcatgca cgaaacctat agcttgtccg ttttatcagg ttcctttcat tcattgccgt
     9781 aaaacggcat ggaattaatc aaaaaagctt cttttttaaa gcgggtgcaa aattacatag
     9841 ttttttcatt ctacaaaatc attcctttga ttttttatag gattttcatc gatccaacat
     9901 taaaaataat tataacatca accgcatcag taatttgata atgctaagat ttcatctgct
     9961 tcccagaatt tctatcgaaa tatagactgt taattattat taatttcaat tagattattg
    10021 atttataata aattacaaaa aatttttatt gttaatgtac cgattgctca atatttaaaa
    10081 aatgaaccga atcaggctgt aaaatatcaa ctctcttgat ttttggtagt tttgccagca
    10141 attgacacct aagaaggatg actgaagaaa aacttcgtat cttgttcgtt tcgcgctggt
    10201 acccccacaa agaagatcca atggaaggac ttttcgtcca acgacatgca caagctgttt
    10261 cgaactatgc aaatgtttcg gttttatatg ctcatcctat tataaacaaa aaaattcttt
    10321 ctccgagatg ggaattctcg acttttcgag atgtggatga gttacgaatc tattattctg
    10381 gcttatcacg aaattttcct ggcagtcaaa ttataaaagc gtcgcaatat ttgtttcatt
    10441 tgatagcagg ttatttatgg ttgcaaaaac gaacaaagcg tttcaaactg gttcatgtta
    10501 atattcttac ccgaacggct attttcgcct ctgtgttaaa aaaccttaca ggattgccct
    10561 acctgattac cgaacactgg tcgaggtact tgcctattac aaatacatat aggggaattc
    10621 tgcggaaaat cgtgactcgc tatgtagtga gaagagctgg tgcaattact gttgtaaccg
    10681 aaaatctcaa aaacgccatg cagcgtcatg gacttcatca taaaagattc tacataattc
    10741 ccaacgttat tgatacgaac atcttcacac ctactttcct aaatcattca agtaaaaagt
    10801 tgctgcatgt ttcctgcttc gaagacaaat cgaagaatat aagtgggcta cttagagcaa
    10861 cacaaaaact ctctcatctt cgtaatgatt ttgagttagt ccttgtgggt gatgggcagg
    10921 attttcaaaa aataaaagaa tacagccaga atcttcattt agaagcttat gtaaccttta
    10981 ctggattaat agaaaaagag gagaaattag ccgaatgtta tcgtcaaagt tgttgtttgg
    11041 taatgtttag caattatgaa aatatgccag tagtcatcaa cgaaagtttg gcctgtggta
    11101 aacctgtgat tgcaacgcga gttggtggaa ttcctgaaat tatagattct gccaaaggta
    11161 ttttgatcga tcctaagaat gaagattcgc tctgtgaagc tatgaattat atgttggacc
    11221 atgcatcgga atatgatagt caggctttac gaaattatgc ggtcgagaac tttagctttt
    11281 cgaaggtggg agaatgtttt ttaaaaattt atcaggaaat aaaacccata caatgacatt
    11341 aggagaaaga aaagggctga aatttggcat tttatgcgat acactgcatc ttcagcagtg
    11401 gcaaacttta gtcatagaac aactgatttt ggaagggaat gcccttgctc tcatcattct
    11461 caacaaagaa gactcgccac caaagtcaaa aggaattcga aggcttttgt gctatcccat
    11521 aaaaaacttt atttttctct tttattatcg ttttttatta aagccaaagt cgaagaggat
    11581 gctttcgata gagccgtgga taaaggaagc tattttattg gaagagaacg caaaaaaagt
    11641 gaaaaaatca tattacttca gcgaaaagac gattgaagct atcaagagtt cggaagttca
    11701 attccttctg agatttggtt tcggaatcat caaaggcgat atccttcatg cggctcctta
    11761 tggagtttgg tcgtttcatc atgacgatcc cgaagtcatt agaggagtgc catcaaattt
    11821 ctgggaaatc ttagaagata tcccggtgaa tggtgctata ttacaacagc tcgacgaaag
    11881 tatagattgc ggaaaaattc tatggcaagg atggtttccc accatcaatc attcttggaa
    11941 agccaacctc gatcaagcat actatggttc ggtagaatgg cccgctcaaa cgtgtagaga
    12001 aattctattc aaaggtgcag actgcctaaa aaacaaacct ttcccaaaac ctgcacccat
    12061 gcgattcgct ccaatcaatt gtataatgtt gaaattcttt ctcaaactat gggcgaacaa
    12121 ggtaaaattt catttcaatg aattatttcg tgctgaacac tggagcattg gatttataga
    12181 aagttcgata aaaaatttta taggaaacaa ttctcgacct gaaaatatca aatggatacg
    12241 tagtgggtca agaagacaat attatgctga tccagcaggg atttaccaga atcatcaact
    12301 gaaaattcta tgtgaacatt acgattacaa accagctcat ggcgagatat gtcttttcaa
    12361 aacaaatcat cattcaacta tttatagacc agaaaaagtt cttctgaatg cctcctggca
    12421 tcttgccttt ccttttattt ttgaagcaga agggaatatc tggtgcttac ccgagtcggc
    12481 ccaaagcaat cagctcaggt tatacagatg ggacacgaaa aaggaagaac ttatgttcga
    12541 aaaaattatt atttccgatt tacaagcggt cgatcccatc atttttcatc ataatcgaaa
    12601 gtggtggctt atgtttaccg ataaaaccaa aagtaattac agtctgcact tatggtatgc
    12661 cgaagatttt agaggccctt attttccaca catcaacaat ccagtaaaaa ctgacattcg
    12721 ttcatcgaga cccgcaggca atatatttta catggatggc cgactaatgc gcccggctca
    12781 ggattgctcc gtcatatcag gacatagaat cacccttaat gaaataattg aaataactcc
    12841 tttttctttc gtcgaaacca ccagattttt cgtagaacct ccaaaatcga ggcagcattt
    12901 caaagggatg cacactttaa atacagctgg accttatgtg atcttcgatg ttaaacaaga
    12961 tctttttatc tgggacaatt tcaaaagaag gcttttgaga aaaacaaaga aaataattgt
    13021 tcaataaaat gaaaagacaa ggctaagtgt ttttctcaca actatcagaa tattcatggt
    13081 atagctaaat tctttgattt tattgcattc gaaaaataga taatcgacct actaataatg
    13141 aaagcggaga aataacaata aaagaataaa agccatagaa ttctctataa tttcatgaca
    13201 ggatgaattg cttggtggat tctttcgggt tagattctca tatagcggat taccatagaa
    13261 tagatgctgg tgaggattcc accaaaactt tcgatgaaat aaactgtata tccattttcc
    13321 atagccaatt taacgagatg cagacgtgag tgacgcatat agtcttccac gctccatgaa
    13381 agataaagat aatcggatga catcatgaaa tatccttgcg gtttgagtac tctatttact
    13441 tcctgaaaaa atttagcagg atgagtaatc aaagggaaga cttgaaaaca acaccatcga
    13501 aagtggcatt ttggaatggg agtgcagtcc catctccaat ccaaacatcg gtcgtatctt
    13561 catcgggtcg aagatggttg atttgatgat gtctgcaggt attggttcca acatactgtg
    13621 caacttccgt aaaaagattt ctatagggtt ttgatcgtgc tccaatatct aagatgtttc
    13681 cactaaagag attttgatag cgatgaattt tattgtgcaa catccagata tttattttgc
    13741 atggattaca tgaaaccact ccttcagaag gtttgccaaa aagatttgaa catgtacaag
    13801 agatgttcat cgagaaataa acaattcttc taattgatat tctgcctgat atttctttgt
    13861 ttccacgcat ttagtgtatt catcaataaa ccggcaatgg tcatattccc gactccaccc
    13921 ggcacaggcg taatccattc agcttttttg cttacctctt cgaaatcgac atcaccaaca
    13981 atcatatagc ccttttcttt ggtattgtcg ggaatgcgat gaattccaac atctattact
    14041 acacttcctt ccttcaccat atcggcagta ataaaacgag gtttacctat ggcaataatg
    14101 agtatatcgg ccgtaagaca aatttctttc aaattctgag tgtaagaatg gcaaagtgta
    14161 accgtagcat tacctggttt tgccttacgg gaaagtaaaa tagagacagg agtaccgaca
    14221 atcatgctac gtcccaacac cacacaatgc tttcctttgg tttctatctg atgacgctcg
    14281 agcatttgca tgatacccaa aggcgtagca ggtataaaat taggttgtcc catcatcatt
    14341 ctccctaaat tataaggatg aaatccatcc acatccttat caggagaaat ggaaagaata
    14401 atcttattag gatcgatttg aggtggaaga ggtaattgca cgataaatcc atcaatatct
    14461 tggtcgtggt tcaaaaaatc aatcacctga agcaactcat tttcagtaaa attttctgga
    14521 taatgataga gggaagaagt aatgccaacc tggcgacaag ctttttcctt agaggcaaca
    14581 tacgaaaggc tggccggatc gtctcccacc aaaacggctg ccagatgagg cggacgttca
    14641 ttacggtcaa ttatttggga tacttcagtt tttatttctc ccttaatatt tgcaaataag
    14701 gttttgccat cgaggagttt catcatgttc gaatatttat aacaaagcta acgaaaattt
    14761 ggtgaattgt tgaattggaa ttattgggga atgttcatct gctcaacgtt ttttctgttg
    14821 tttcatattc ttgagcattt gacgaggacc gcccgtggtc accatcttca tcattttacg
    14881 ggtttcgtcg aattgtttca ttagcctgtt aacttcggtc acatcggttc ccgagcccat
    14941 ggcaattcgt ttacggcggc ttccgttgat gatggcggga ttttgccttt cttctggtgt
    15001 catcgaatga ataatagctt ctatactttt gaaagcatca tcgtcaacat cgaggtcttt
    15061 caccattttt cccataccag gtatcattcc catgaggtct tttacattcc ccattttttt
    15121 aatttgatga atttgtttga ggaaatcatc gaaattaaat tcgtctttgg caattttacg
    15181 ttgaagtttt ttggcttctt cagcgtcgaa ttgttcctga gccttttcga cgagtgaaac
    15241 gatatcgccc atgccaagaa tacggtcggc catacgctcg ggatagaaca catcaatggc
    15301 atccattttt tcaccaagtc caacaaattt aatgggttta tccaccacag ccctaacggt
    15361 taatgccgca ccccctcggg tatccccatc cagtttggta agcacaactc catcataatc
    15421 gatcacctca ttgaatgctt tagcagtgtt tacggcatcc tggcctgtca tggcatccac
    15481 cacaaaaagg gtttcctgag gattaacggc ttttttaatg gcagcaatct cgtccatcat
    15541 cagctgatca atagccagac ggcctgctgt atcaataatg actacctgat ggcccgattt
    15601 atcacgtgca taatttacgg cgttgcgagc aatttttacc ggatcggaat tgttttcttc
    15661 ggtataaact tcgacgtcaa tttgttggcc gagcacattc aactgttcga tagcggcagg
    15721 tctataaata tcgcaagcca ccaaaagagg cgaacgacca tatttggttt tcacataatg
    15781 ggcgagtttt gccgagaagg tagttttgcc cgagccctga agccccgcca tcaatactac
    15841 tgcaggatta cctttcaggt tcaggtccga tttctggcca cccatcaggg taaccaactc
    15901 atcatggaca atctttatca tcaattgact gggtgagatg gcctttagaa catcctgccc
    15961 taaagccttc tgcttcactt catcagtgaa ttgcttggct attttatagc tgacgtcggc
    16021 atcgagcaat gctttacgga tttctttcac cgtatcggca acattaattt cggtgatgct
    16081 tccttgacct ctaaggactt taaaagcacg gtcgagtttt tcagtaagac cctcaaacat
    16141 aaatcaacga cattaagaca aataataaga attcatgtat tacacctccc attgaacact
    16201 catggcttcg gcttctttat cgatcttttt taccagaccc tgaagtacgg tacccgggcc
    16261 aacttcgata aatatctttg ctccgtcggc tatcatattt tctatagttt gtctccagcg
    16321 cacgggagca gttagttgtt ttataagatt agctttaatg atttcagggt cggtaacagg
    16381 ctggccggta acattctgat agattgggca aattcccttc acaaaacgtg tagccgaaat
    16441 ggcttcagcc agttcgacac gtgcaggttc cattaaagga gaatgaaaag ccccccccac
    16501 ttttagagag agagtacgct tggctccagc ttccttcaac ttctcgcaag ccatagaaac
    16561 tccaacaatg ctgcccgaaa tcacgatttg tccgggactg ttataattgg caggtaccac
    16621 aatctcatcg ctgatgctgc ggcaaatcct ttccacattg gaatcgtcca gtccgagaat
    16681 agcggccatg gccgagggat tcatctcaca ggctttctgc atggccatgg ctctggcata
    16741 gactaatttc aaaccatctt caaacgtcaa taccttattg gccaccaaag ccgaaaattc
    16801 gcccaatgaa tgacccgcca ccatgtcggg tttaaactcc tcacccaaaa ccctggctaa
    16861 tataacactg tgtaggaata tggctggttg agtaactttg gtctgcatga gttcgtcttc
    16921 actaccctga aacataatat cggtaattcg aaaaccaagt tgagaattag ctttttcgaa
    16981 aagctcgcgc gcaagctccg atttttcgta taaatctttg cccataccgc taaattgagc
    17041 cccttgtcca gggaaaagat atgctttcat gaaatgatag gttatgttaa attaaagtga
    17101 aattcgattc ttctaaattt actattcaaa ttcagaaata tactatttct taataatttt
    17161 gaaacgaggc ggcaaaagta agaatatttt tcgagccgaa tccttctatg taaatttcga
    17221 ccagattggc aaatattata tttttgcaat cgaaaatcaa tggaagatta tttatgttaa
    17281 gcgaagaaag cgaattttgt ttggaagaaa cacgtgaaag catggataat gcccttttgc
    17341 atttcgaaaa agaatttcag aaaatcaggg caggcaaagc cacacctcag atgctcgatg
    17401 gagtgaaagt ggattattac ggagcaatca ctcctattga caagataagc aacattacca
    17461 ctcccgatcc tcgccagatc attgttcagc cgtgggacaa aacgatgctt gcactcatcg
    17521 aaaaggcaat tcttgctgca aatcttggtt tcaatcccca aaacaacgga gaggttctgc
    17581 gaatcatcgt tccacctctt accgaagaaa ggcgcaggga actggtaaaa aaggcaaaag
    17641 ttgaagccga aaatgcaaaa gttacgattc gcaatattcg tcgcaatgcc atggaaactg
    17701 caaaaaaact caaaaaagat ggcatacccg aagatgaaat cgatcagttg gaagaagaaa
    17761 tccagaaaat aaccgacgca tacatcgaaa aaatcgataa agcattcgaa ataaaagaaa
    17821 aagacattat gactgtgtga gtcgaaagtc taaaattttt acctcactcc attgtttcgt
    17881 ttttattccc cacagttacg acaaatctca taaacatctc tcgatttcat cacattttga
    17941 cgaaatattg tagcatttgg gcttgtccat atagttttaa cctgagttga agaatcgaga
    18001 agcccataag aatggacagc atctttatca taacaacacg gaatcagtct accatcccag
    18061 gtaatcactg acccttgcca cattctcctg cagcctcttt tacgacggtt cttcatgagc
    18121 aataaactat cttctccttt aatatatcgt gcaaattcag gtatatccgt aagaagacca
    18181 ttctcatccc caggactgta gatttgagct gatttaagtt ctaccgcatc gacccctaat
    18241 tgtttcccca ggctttgaat tttatttatt tggtgttggt tatgtttcat tacgataaac
    18301 tgtaacctga taaaaggatt ttttcgattc gaacgttttc gggcggctac aagctgccgt
    18361 attccttcgg tggctttatc gaaacttcca ccttttctat atttttcata agtaagggaa
    18421 tcggttccat cgagtgaaat cacaatctcg tccaaacctg ccattataat attctcagac
    18481 agttcggaag ttaaaaaatg tgcattggta gaagtgatgg tataaattct cttttgatga
    18541 gccattttta ccatagcagc aaaatcttcg ttcaaaagag gttcaccctg aaaatacaga
    18601 atcagataaa aaagttcggg agaagattca tcgagccatt gtgcgtaatc atgaaggcga
    18661 agcaagcctg ccgaacgcgt aaggctatgg ctgccgacag gacattcggg acaatgcaga
    18721 ttacatatac ccgaagcttc aatagaaagt gtccatggaa aaccccaatg gatatatcta
    18781 tcaaaccatt gggagatgga ataacttgcg taatttttca ggaaattcca agaacgtcga
    18841 aaagtgaggc aacgtatgag cctgatccat gaaaaaatgc gcatcaaatt catcatcaaa
    18901 gatttcgtgt aagtttatcc atcaggtcga aactgctcaa aaactccttt cccactatac
    18961 gaatcaaagt ataagccgga acggccagaa tcattcctat tatccctcca accgaaccgc
    19021 ccatcatcat aatcaagaaa atctcgatgg gatgagcatt tactctttta gaataaatct
    19081 gaggttgcag cacaaaatta tccaccatat taactgctat aaaaactcca accacaatga
    19141 ggggttgaac ccagaaggtg gcatatgaac cctcagccag cacccagaac agtgtaagca
    19201 atactccgag tgcgccacca atgaaaggtc ccagataggg tatcaaattg aaaaatccac
    19261 ctataaaacc tatcaataat gcattgggga ttccagcaat catcatccct accgaaagaa
    19321 gaaccatcat gataaatact tctaaaaaca ggccaccaac atatcttccc aacaaatctc
    19381 tactggtatt cagtacacta atagctctcg tttcatattc ataaggcaca aacagaagaa
    19441 aaagttgcat taataactta tcatctcgca aaaagaaaaa tgtaagaaaa gctactgcaa
    19501 aagctcctac ggcaaaagaa gatacaaatc ccgtaaggct tcccaataat ttataaatag
    19561 aactaaactc aaaaatatca cccaatcgtt gaacaatcgc ctgttccaga ttctcgccag
    19621 aaggtagaag gtgatattga tgaaggaaat tttcgatatc ctcgagtaaa ggagccaaat
    19681 taactgccag cgtattaaaa tcaattttgg taattacaag agcctgctgg taaactatag
    19741 ccacaaaccc atacaataaa atgacaacaa ctaaaataag aatcagcaaa gtaatcaagg
    19801 aggatgccca ggctggaagc ctgaatcttt tgaaacctgc ccctcttaat tttctttcga
    19861 taggcctacc caacagtgta aaaacccctg caatgccaat gtaccaaaaa acaaaggtga
    19921 aataataaat caaaaccact ataatcagca gggtaaggac taacatccag cgccttgaat
    19981 gtgaagccat cgagattagc tttataggaa gcggtttatt cagggcaaag ataatcgagc
    20041 tttatcaaac cacaaaagat aaaaacctcc catgagtatt cgaggggagg tttatcagaa
    20101 atgcacggaa aaattctcag attaatttac gggctgcagc tattgccgca tcatagtcag
    20161 gatgatgggt attttccttc acgtactcta tataacggat gatgccctcc ctatcgatca
    20221 caacgattcc acggttgagt aaacgcagct cttctatcaa aaaaccatat ttcaagccga
    20281 aatcggtatc tttatgatcg gatagagtga tgaccttatc gatgccttct gcggcacaaa
    20341 atcttcccaa agcgaaagga aggtcgcagg agatggtaag gataacaacc tcgcccaaag
    20401 aagcagcttc ggtattgaaa cgtcgggtct gggcagcaca tacacctgta tctaccgagg
    20461 gtacactgct gatgataatc actttaccct tgaaatcgct cagatttaca ggttttgaat
    20521 cggcggctaa agcagtgaaa ttaggagcaa tctcacctgg ctgcaccata tcgcccagta
    20581 gtgtcaccgg cttgcccatg aaagtagtga tccctttatt ctttttcatg atgggtcaaa
    20641 ttaaattgat attattaaga tatatgaaat aaacaaaaat ttccaatgaa agttcaggta
    20701 aaaaaacaaa atataggcac attcctttaa tccacgaaat cgaaatcctc gtcattctta
    20761 aaatatcgaa taagaaccta attcgtctga tgacatccaa caaaacaacc ttgccatttt
    20821 gtcagagtat atcgggctgg cacaggcttt gccaatggaa tagcgtttca aaaaatgtca
    20881 attatggcta cacagcaaaa gggtaaaatc aacgtacaaa ccgagaatat ttttcccatc
    20941 attaagaagt ttctgtattc ggaacatgag atttttttac gtgaacttat ctcgaatgca
    21001 gtagatgcga ccacgaaatt gaaggcactg gcttcgatag gcgaatttaa aggggagttg
    21061 ggcgatcttc gtatagaagt aaaagtagat aaaaagaaaa aaactctcac cgtatccgat
    21121 aaaggcatag gcatgacagc cgaagaaata gacaagtata ttaatcaaat tgcattttcg
    21181 agtgccgaag aatttatcag ccgcttcaag gataagaacg ataccgaagt cagtcagtta
    21241 atcgggcatt ttggtctggg attttattcg gcatttatgg tagccgataa ggtaaggata
    21301 gaaagtttat cgtggacaga aggagctgaa gctgtggtct gggaatgcga cggttcgccc
    21361 gaatatgcga tgaaaccctc aaataaaaaa actcgtggga ccgatgtaac cctatatata
    21421 aataaagata acgaagaatt tctcgacgag tacagggttc tggaactatt gaagaaatat
    21481 tgccgttttc ttccgatcga gatcgttttt ggaaccgaaa aagtaagaga aaaggtagaa
    21541 ggcgaaaaag acaaagacgg caacgatgtt tacaaggaag tcgaaaaacc tcgcatcatc
    21601 aataacacca aacccgcctg gacaaaaaaa ccttcggaac tcaccgacga ggactataaa
    21661 aatttctatc gtgagctcta tcctatgaat ttcgaagaac ctttgttttg gattcatttg
    21721 aatgtggatt atccttttaa actcactgga attttatatt tcccgaaaat caaggaaaga
    21781 atcgaggttc agaaagaaaa gatacagctt tactgcaatc aagtatttgt aaccgatagc
    21841 gtggaaggca ttgtacccga atttcttaca cttttgcatg gtgtcattga ctcgcccgat
    21901 atacctttga acgtttcccg cagctacctt caaagcgatc ctcaggtaaa gaagatttct
    21961 aaccatatta tgaaaaaagt ggccgataag ttagaggaaa tcttcaagaa taaccgtgaa
    22021 gaattcgaga aaaaatggga cgatatcaaa gtctttatac aatatggaat gattactgaa
    22081 ccaaaattct acgaaagagc cgaaaaattc tacctcttca aggatatcga ccataaatat
    22141 tacactttcg aagaatacaa agccttgata aaacctttgc aaaccgataa ggaaaaaaat
    22201 ctggtatata tatacagcaa taatccggaa accgaatatg tattcatttc ggctgcaaaa
    22261 gaaaaaggct acaatatttt gcttatggat ggaatcctcg acaaccattt catcaatcat
    22321 ctcgagatga aattcgacaa atgcaggttt gtaagagtag attctgatgt catcgaaaaa
    22381 ctgattccca aggaagaaga aagcatttct aagttaactg aagaacaaaa agaaaacctg
    22441 aaaaagcttt tcgaagaagt catcgacaaa aataatttca ctctcgtgct cgagaatctg
    22501 tcggagagcg acctacctgt tttcatcacc aagcctgaat acatgcggcg tatgaaggat
    22561 atgtcagcct tgggaggggg catgtcctat atggatgctc ttcctgatca gttcaatctg
    22621 gtaattaata ccaatcaccc ggctattctg catattctcg acgaaaacga cgaggagaaa
    22681 cgcaagaatc tggctcatca actgaaagat ctggctatgc tgcaacaagg ccttttaaaa
    22741 ggcgaagcgc tgaccgattt tgtaaaacgt tcggtagaat tcattcgcta aaaaaccctc
    22801 caacttgcca agaaaaaacg attgactcaa taagaggatg attcatctct gattttgatt
    22861 ttttttgact tcaaatacaa aaagagtact tttgtaggaa gtaattcttc aaaataacct
    22921 ttaaattcaa atttatgaaa aaaggactga taattggatt gtcgattgcc attatcgtac
    22981 tgatattcat tttaacaggc gtttcgatgt acaactcgct ggtaaaagct gaagaaaatg
    23041 taaatgcgca gtgggcaaat gtggagaatg tatatcagcg ccgtgccgat cttattccca
    23101 atttggtgaa tacggtgaaa ggttatgcca atttcgagca gaaaacgata accgatgtca
    23161 ttgaagctcg tgcaaaagcc acttctgtaa acatcaatcc cgaaaaattg aatgaagcca
    23221 gcctgcagga atttcaaaat gcgcaggaac aactgagttc agccctgagc cgtttgatgg
    23281 ttgtggtgga acgttaccct gagctgaaag ccaatcagaa ttttcttgaa ttgcagtcgc
    23341 agctcgaagg taccgaaaac cgcattgctg tggaaagaag aaaattcaac gagatggctc
    23401 aggcgtataa caaaagcata cgcacctttc ctcgaaacat cattgccaac atgtttagat
    23461 tcgaaaggaa agcctatttc gaggctactg aaggagccga gcaagtgcct aaagtcgagt
    23521 tttaaaaaat ggctctctta aaaataaaat cggtacgcga ttattttaca cccgaggaac
    23581 gcgaacgcct tacagaagca gtaaaagaaa tggagctgaa aacctcggca gagattcgaa
    23641 ttcatataga gcgaaaatgc cagggtgatc cactcgaccg tgctgcctat atttttggga
    23701 aacttggtat gcataaaacg gcacttcgca atggagtact gatctatctt gccatagaaa
    23761 atcaccaatt tgctttcatt ggagatagag gaatcaatgc ggtagttcct cccaacttct
    23821 ggacagattt aaaactaaaa gcacagaaat atttcagtga aggacaattc acacaaggtt
    23881 tgctcgaagt gattgaagag atcggtactt atctccaaaa attctttccc tataaagaaa
    23941 acgatatcaa tgaattaccc gatgagatct cattcaaaga taaaccatcg aaataattcg
    24001 aagcctattt tgcaagacaa ttcactgagc aaaggcctta gaactttagc atttcttcta
    24061 ttcacctctc tttttttcat tacaagcctg aaggccgacg acctccctcc tcgtcctttc
    24121 cctcctcgcc tggtgaatga tcttgcagga atcatcgatc cttacgccat cattcaactc
    24181 gaacaaaaac tggtagcatt caacgatact acatccactc aaatcgccgt agttactgtt
    24241 ccttcattat gtggttatga tattgccgac tttaccgacc gtctggctga aaagtgggga
    24301 gtcgggcaga aagggaaaga caatggggtg atgattctgg taaaacccaa gaacaatgag
    24361 gggaatggct atgtgcgtat atcggtaggt tacgggctcg aggatgtaat taccgatgca
    24421 gtagcccgaa gaattataga aaaagagatg attccctatt tcaggcaagg tgattataca
    24481 aaaggaatta tggctggcac ggaagtaatc atgaatcttt cgtcgggaaa atttaaagcc
    24541 gaagattata ttgggggaga cccgaacgct gccattggca tagccatctt cattattata
    24601 ctcggtggaa tttttctgat gcgattttta ttttcaccct ttgcaattgg ccgatcctcg
    24661 tccttctggc cgatgattgt tttgatgaac atgtttggcc gaggaggtca taaggggaaa
    24721 tgggacaatt tttcatcggg aagtggatgg tttggtggcg gagggagcag cttcggggga
    24781 tttggaggag gtagcttcgg tggtggaggc gcttcgggaa gctggtaaaa aatttattcg
    24841 ccttccctta ctgaaatttt gaatcttata ttgaatcact cctcttagga cgctttattt
    24901 atggggattc attctttaaa atcaaataag gcctcgtttt ctgagtactt atcaaaaaat
    24961 tcgtaaaaaa ttcataatga ttcattaaga taattacatg gcaagcgaat gtgttctatc
    25021 ctttgccctc aaaatcaata tcgaactaat agaccttact cgaatcgatg gtagaagtta
    25081 gaaaacaggt tatgtttagc gaaataattc gcttctatgt agatggtttt aaaaatacga
    25141 aggcaagtaa gcaactgggg acgatcatgt ttactaagta gttcatcatg tttgccatat
    25201 ttcaaatcag tactaaccga ccagaaattt gaataagaaa gtgggaaaat tcatattcct
    25261 acccaatact gaagaaaatt aatttctatt tacagaattt gtgatgaata ttttaatttt
    25321 tcgcccatag tcaaagaaaa gacaaaaaat ttacttgctc agcacagttt cgactttttc
    25381 tgtttttgaa taagttgccg ggaaatattc ttttaggagt aaaagcagga attttcttca
    25441 gcggaagcca attgcagatc aacgaactca aacccactcc acttcttcct cagctgggtt
    25501 atcctgttat tttgtaacta acaggtcatg caaatgatct caaagctgtt ctgaattttg
    25561 ccaatgtcac tttggatctg gcactccgtt tcctaatctg cattcctggc tcactttgaa
    25621 tcatcgaaac catagaacat gatagcattg acgaaagaag tctataaaga atcagtgatc
    25681 tcctccccat tcttacttgt ctttctgaag ttcttgattg gtatcctcac acgaaaaagt
    25741 tttgtagccg atcctgttat aaaagtgatc catctcgaat cttataaata tttacaaaat
    25801 tatctgcaga taccgatggc gttgattatc cttttttgcc cttgttctgt gagataatgg
    25861 ccttgctctg taaaaaaatg acaaaaaaat gttgatttac aaggcctggg gtcgtagtca
    25921 ttgtcctcta actttttctc ttagctagtt ataaccattg gcaatctatt caactttaaa
    25981 ttggttgctg cgggaagaaa cggaggattt ctttgcatcc ctctagagtt ggcttatctt
    26041 tcggaacagc tcgaacagct attattcctt gatgaccata agttatgtct gtttgcttat
    26101 acccttcagg attggataca tcttttgggc atagaaatct ataaggaaaa gtgagggaga
    26161 aaaattagaa aaaagaacaa ttgcttacta actcataact gtcgagctat gatcatccgg
    26221 gttgataaca tgaccatgtt ctgttctgct ggtttataga atttcaaaag gaacttcgcg
    26281 aaaatcgggt ctctatcaga acaaatcatc caaattcagg aaagattctt gaaatttttg
    26341 ataaaagcca gattttactc ctatgttctt tgaataaaac ttctagtttt tttatacttt
    26401 tttgtatctt tgacaattcc aaactttata tttttttgaa tcatgaaaaa tttacgattt
    26461 tttatcttcc ttctggctat ttcattcata cctgtaattt tacgtgccca gacagaggag
    26521 tcggatactg cttctttccc ttattggata gaaatgatgc aagacgagaa tatcaatttc
    26581 tatcaagtgg taaaagcatt cgatacttat tgggaaggca ggcctgtgac taaaggctgt
    26641 ggatataaac cattcaaacg ctgggaatat tcgatgcgta atggaagaat ctacccggat
    26701 ggtacacgta agccggcttc ttacacttgg aatgcttatt tcgattatat caaatcatat
    26761 agagactcgg tttacgggca ttgggaatcc attggtccta aaacttatcc atctttgggg
    26821 tataaaggtt taggcagaat taatgccatt gcttttcatc ctacgaatcc ggatattttt
    26881 tacataggtg ctccttcggg agggttatgg catacttacg acggcggcaa cacctggggc
    26941 tcgaatacag ataatcttcc cacacttgga gtctcggcca tcattgtaga ttactcgaat
    27001 ccaaatttaa tttatattgg ttcgggcgat agagatgcca acgacgctgt aggaatgggc
    27061 gttttgcgta gcaatgatag tggattgaca tgggaactat ggaataatgg aatgggcgaa
    27121 aaaatcgttg gccgtctgat tcaacatcct acaaatccat ccataatatt ggctgctact
    27181 tcgggtggaa ttttcaaaac aatagacgga ggagccaatt gggcaatcaa aaaaacggga
    27241 aatttcaaag acatagtttt caaacccaac aatgcggata ttatctatgc ctgttcggga
    27301 gcgaattttt tccgttcgac cgatgggggt gaaaatttta cacaaattac taacggattg
    27361 accggagggc agcgcgccgt tgttgctgtt acacctgcta accccgaata cgtttatttg
    27421 ctggtttctg ataatagtag tgggttcaag ggtctttatc gttctaccga tggaggaact
    27481 tccttcgtga ccatgagcaa tagcccgaat atactggatt ggagttgcga cggcagtggt
    27541 tctggaggac aagcctggta cgatttggaa attgccgccg accctatgaa tccgaattta
    27601 atctatgctg gtggtattaa tatttggaaa tcctataatg gaggagaaac ctggcaaatt
    27661 acggcccatt ggtatggagg atgcaatgtt caggcagtcc atgccgacca ccacgtattt
    27721 gaattctctc ctattcatca tcatctttat gtgggtaatg acggaggaat ttattcaaca
    27781 gccaatcagg gtcaaacttg gactgaatat accagtggtc tggtaattag ccagatttat
    27841 aaaatcggac aggatgtagc cgacacttct catttgatta ttggtatgca ggacaatgga
    27901 acatccactc taatcaataa tgtttgggcc gatacaagag gcggagatgg aatggaatgc
    27961 atcatcgatt atcaagagcc agcctattca tacgcttcat tatattatgg cgatattgca
    28021 cgtttttaca ataataatgg aggttacacc atagcagcta aaggtaaatt tggaattacc
    28081 gaagatggcg actggattac tccttacact ttgcacgtta ctgatcccaa cagaatgttc
    28141 attggataca agaacatctg gcgcggtcaa tatattcgta ctaccccaca atgggttaaa
    28201 atttccgaca atctcgcagg taacaacaat acaaatatcc gtatagttga gcaatcacag
    28261 gcaaatcccg atatattata ttttgcccgc tgggacaata agctcttccg taccgacaat
    28321 gccaacgcag gtcaacccgt ttgggtgaat cttacatcat tattacccct ttcaggccag
    28381 acaccatctg ctttaaggcc tcatcctacc aaaccagata tcatttatat ggccttgggt
    28441 ggtaaaatct ataaatcaca aaatcgcgga caaagctgga ccgacatcag tggcagcttg
    28501 cccaacgtgg aatattcttc cattgccttt tacattaggg ctcacgaagg actttatgta
    28561 gcttcagata tcggggtatt ttacaaggac tctactctga ccgactggat acaattcagc
    28621 aatggactcc cagtggatgc atccattcgt gagatcgaaa ttgcctataa caatcaaaat
    28681 aatgaatacg accgcattag agcaggtaca tatggtcgag gcgtatggtc ttcgagcgtt
    28741 taccgtactc cacctacagc ttctttcaca tccgatgtaa tcatagtgcc tgttacaggc
    28801 caggttcact tcaaagatat ttcgagtggc attccaagtt catggcaatg gactttcgaa
    28861 ggaggtcaac ccgcaacttc taccattcaa aatccagtaa taacctacaa caacccgggc
    28921 tctttcaaag taaaacttat cgtaagcaat tcattaggaa tcgactctat cgaatttcag
    28981 aactatatta ccgtaagtaa taccatttta cctcttgttg ggttttatgc cgataacact
    29041 attccttgtt cgggacaaat cgttcggctt tacgatacta cactctatgg gcctatttcg
    29101 tggcattggc aattccagcc aaataccata gcttatcaca atggaacttt ctataattcg
    29161 caaaaccccg tggttcaatt caacgaaaat accgaataca ccataacact tacggcttcc
    29221 aatccgaatg gtacatcttc cgtaacgaga caaaattata ttcactcagg aggttatctc
    29281 atgcctttct atgaagattt cgaaactttg aattttgctg atcgttcatg gacagtggaa
    29341 aaccccgata tgggaattac ttgggatatg gctcaggtcg aaagcattca cggacccact
    29401 gtggctgccc gaatgcagtt tttctactat tatctaatga accaacgaga ccgcctgatt
    29461 agtcctccac tgaatttctc gaatgaaaac aaagtaatcc tatcgttcga acatgcttat
    29521 gcacagcgct ataatcaacg agattcttta atagtgctgg cgagtgatga ctgcggaagt
    29581 acatggacaa gaatatttgc ttcggggccc gatggcaatg gcatcttcga aactgcccca
    29641 aaaaccgaat cagaattcat tccatctacc gccgaggact ggtgcggctc gggctgggga
    29701 gcaaattgca tccagctcga cctaagtgct tgggccggca aacccaactg tcgcatcgct
    29761 ttcgaggcat tcaataaatt gggcaataat ctttatatag acaatattgt tgtaagccgc
    29821 tcaggtgtgg gaatggctca ttccgttgaa aatactttta tcgttagccc caacccttcg
    29881 aatggaatct tcaacattac aaccccctat cataaccaac aaagagcaga aatctattcc
    29941 accgatggac gtatgctgtt ttcaaaagat ttcagcaaca acaacctgaa aatcaatgtt
    30001 tcttcttatc cctcaggcat ctatacatta aaaattatag ccaatcaaca aacctatcag
    30061 atcaaattaa tcaagaaata gagaatcata actgagatgg tctgcaggtg ctacaaatct
    30121 tgcagaccac ttccgttatc gctcaaatcc tattcgaacc tcactcgtcg aatatattct
    30181 gtcataccct atcgttgctc tttcgaatga taatctttgg ctcattttct tcatgcgtcc
    30241 tcgaaattat cacttcaaaa taatgtggtt caaattttta tctctttaaa caaaaccgaa
    30301 aacaaatgaa aaaattgcag tttttcattt tgacagtatc tatattcgtt ggcttgttgc
    30361 cctatcgaag tatagcacaa ggctggcgta gtaatgaaat ggaaataaat gttttggtaa
    30421 aaactctgga agatatggaa ctcattcatc gttcaggatg gagttacgat cgaccttcat
    30481 caattccggg catagccaga ctttatctca ttcccaaaga actcgaagaa ttaaaacaaa
    30541 tgggcatgga atattccata agtattcccg atattgcgga acattatcga gacctttggg
    30601 gaggggttgt accgccagga tattacactt acgaacaatt gattgcaatc gaggatagtc
    30661 ttgctgcagc atttccagct atttgcaaaa aaatcgtcat aggtacaagc gttcagaatc
    30721 gtcaactggc agttttgaaa atcagtgata atgtggaaca agacgaagcc gagcccgaaa
    30781 tatttttcga agcaggcata catggggatg aagtgggagg ccccgaaaac ctcatcaggt
    30841 ttgcccgaga cttatgcaag gggtatggtt cgaatccaca aattactaat ctggtaaata
    30901 cacgtgaatt attcatttta gtaatgacca atcccgacgg acgggttcag atgagccgtt
    30961 acaatgccaa tctggtagat atcaaccgag atggaggtta tatgtgggat ggtgagggca
    31021 atagccctgg tgctttttct cagacggaaa caaaagccat tagagacttt gccgcacaaa
    31081 accagtttgt cgtttttacc gattaccaca gcgggacaga gtttataagt tatccatgga
    31141 gttacaggcc tcagatggca cccgatcaac ctcatttaca ccaacttgct catgtttatg
    31201 ctaccaattc cggctacacg aatattccct atgcctctgg ctacagtggt atgtatccta
    31261 tcaatgggag cactaaagat tttaactatg gattgcaggg aagcatttcc tggtcgatcg
    31321 aaatctcaaa taacaagcaa ccgcctgctt ctcaaatata ttattattat ctaaagaaca
    31381 aaggagccat gattgcaatt tcagagcaat gtggcaaagg aatctatggt gtcgtaacag
    31441 actcggttac gggaaatcca atcccggcaa aaattcgtat tggagggact gttcctcttt
    31501 ataatgatcc tgtggtcgga gattatcaca aatttgtggt accggggatt tacactgttg
    31561 aagcatctgc caatggatat aagacgaaaa ctattacaga tgttcaggtt cttcccgaca
    31621 gttcggtggg ggtagatata gcattgagtc caaagcaagg ccattatgca tttcagataa
    31681 aggcttgcag gattcccggg aataatttct acgatgaagg acatacctgg gcggctctcg
    31741 gagaacagga tggaataaac tattcccttg ggaagaatgg ttggattatt ttggatatgt
    31801 atgatacgat ttataatggc aatggcaacg atatattagt ggtagaagga gattcaagcc
    31861 ccgaatcgta tagtgtgtat gcagctataa ctcaggatgg gccttggaaa ttgatgggca
    31921 gtgctagcgg aactcatgaa ttcgatctaa cttcagccaa cctgaacaaa gcaaggtatc
    31981 taaagattgt ggatggtggg acgggttcgt attttggcga agatgccggt ttcgaccttg
    32041 atggagtaaa agtgctatac attccggttt ctgccaattt taccgcttcc aataccatgc
    32101 cgtgcattga caatagcatc gatttcgccg ataattcttt gggtaaccca acgatctggc
    32161 agtggctctt ccaaggcgga aatccatctt cctctaactc ccaaaatcca tcaggaatca
    32221 tgtatccaaa tccgggaatc tatgacgtaa ccctcaccgt aggtgatggc gttacttcct
    32281 cttcccttac aaaatcagga tatatccatg tttatggaac gcccttgcct ccattgactc
    32341 ctctgggaga tagctcattt tgtcagggtc tttcacaaag tgattacact accgctggag
    32401 cacctaatgc cacctcttat gaatgggtgc tgaccccaac cgatgcaggc atcattacgg
    32461 gcaactggat cctcgctacc gtaaactgga atcccaattt caatggtcag gcaatggtaa
    32521 aagcaaggga gacaagccct tgcggtactg gccaatggtc attacctaaa gtagtcgatg
    32581 tgatggaaag acccatagtc gatctcggaa acgataccat tattgcagct tcagcttctc
    32641 tgttgctcga tgcaggcaat ccgggatgct catatctatg gaatacaaat gaaactaccc
    32701 aaactattat tgccgatacc aatggcattg gagcgggaat tcatgaatat tgggttcgag
    32761 taacttctcc aaatctttgt tcgagctccg atacaattct tatcaacttc agcatcgaca
    32821 taggaatgcc tgatttttcg gtttcttcaa ttaaagtctt tcccaatcca accactcagg
    32881 gcttgtggat agaactgaac gaagaaattg gcatgtcgga tatatttttc atagacacta
    32941 agggagagaa atggaaattg cctgtcagta tttttcataa agacacttta ttttatattt
    33001 ctaccaaaac ccttccttca ggaatttact ttctaagaat tgaagcctcc gcaaaggcct
    33061 taacgatacc cattatcaag cgatgaagtt gcgttaagta gtaagggttg cgaaaaacat
    33121 agtaattggt gaatggggtt gaggtttgat gagtatgaag ttaaaagcta aaagttcata
    33181 aagttgaaaa tggaggaaag gaggacagag aaaaggagaa tggtggatgg tgattagtgg
    33241 atagtggatg gtggttagtg gatggagttt gttagttgtt agttgttagt tgttcgtttg
    33301 agtttgggat ttgtttgtaa tttggaattt tatggttgtt cgttgtcagt tatttgttgt
    33361 cagttgaaat ttgcttgtaa tttggtggtt ttttagttgt taaaagaggg tggatcagag
    33421 agaaaaataa aaatgttctc acagatatag cagaaaaaag cccttgcaga tgttcacaga
    33481 aaatcagcgt aaatcagcaa gaaaaaataa agttctccca actttgatag tccccttgtc
    33541 tcttcgtctt aaatcctccc attcttctcg tcgctttatc tcgaagtctt aaagcaaact
    33601 ttacagaact tcgttgaatt ggctgcgcaa cccttcaatt aaaaaggggg ttggaaaatg
    33661 aaaatttaaa cttagaattt tcgttggaga agttgaacaa taaagccaat ctgaatgaga
    33721 gcatctttgc gatgatgtat tatgctataa tccaaataat tattctattt atttactaac
    33781 taactttgaa cttgtcatct attcgcaaat cttggataaa aaaatttgta tagaaataaa
    33841 attatatttg accttttatc gttgagaata atttctgcac aatcgaaata caaaagaaaa
    33901 atggtgaata aatacataat aaaaatgctt tggaatttat ttattaaatt tcaactttct
    33961 gataaaaatt gttttacctt tcccttcatc tattccgtct tagaaatgtt ataaaatagt
    34021 tgaataatta tctcaaactc gttatatatt tgcggtataa aatttttgac tatgcctaag
    34081 gcgtgttttt atttaaataa ttcgttggca aacagcatca atgaacatgt agatatttcg
    34141 gttgcaaggt gttatcaatg cgggaaatgc tctgcgggct gtcctattgt tgaatttatg
    34201 gattattcac cgagtttggt gttacgatat cttcaaactg aattacccga aaacgatgat
    34261 gctgtcttgc gttccaagac aatttggtat tgtttgtctt gtgaaatgtg tattgcacgt
    34321 tgtccgatgg aagtggacat tcctgttatt atggattttt taagaatcga atcgatacga
    34381 ttaagaaaaa cccataaaga agtaaagaac atcatcagat ttcataaagc ctttctgaac
    34441 accattaaac gtaatggaag gctgcacgaa atggggttag tattggatta caagacgagg
    34501 tcgttagatc tgcttcagga tctggcttta tcgcccggta tgttcaaaag aggaaagtta
    34561 agaatattgc ccgaacaggt caagaatcgc aaagaaatta aacatatttt taccataggg
    34621 tcgaataaac gaaagaataa atcgataaag aattaacgca tgaaacttgg attttatccc
    34681 ggatgttcac tggagggatc atcgagagaa tataacgagt cgacgatagc cattgccgaa
    34741 aaattcgata tagaattagt tcaaattccc gattggaatt gctgtggtgc tacagcggct
    34801 cataatttag ataaaaaatt atctttattg cttccggcac gtaaccttgt ccttgccgag
    34861 gaagggggat tgaaagatgt tgttgtccct tgtgcagcat gctttagccg attgagtacc
    34921 acacaccatg aattattact tgatgaagaa ttgcggcatc aggtcgataa tattttcggg
    34981 aaacattata agggaagcat ccgcatttta aatgtcattc aatttattca tgaatacatt
    35041 ttagagaagc tggatgaaaa gattgtaaac gaatttcgga ataaagtagc ttgctattat
    35101 gggtgcttac tggtacgtcc tcataaaata ctgaattttg acagaaccga agatcctcaa
    35161 actatggatt tggtgatgaa aaaattaggg gctgaaccta ttgactgggc atataaaacg
    35221 gaatgctgtg gtgcaggatt ttctgtttca tttaccgatg ccgtgtcgaa gctatcggga
    35281 aagatcgttg aagacgctat tcacagaggc gccgaagcca tcattgttgc gtgcccaatg
    35341 tgccaatcca atctcgatat gagacgatgt ggcatcgata aatatttagg gaaaagaacc
    35401 aatatccctg tattatatat tactcaagct attggtttgg ctttaggaat gaatatgaaa
    35461 cagcttggat tacatcgtca tttcgttcct gttaactttg ataaattcaa gttaattaag
    35521 aaacaaacca aagtcgaaga accacaaaaa atggaggaaa aataatggca cgaataggag
    35581 tttttatctg tcattgtggc gaaaacattg gcgaaaccgt cgattgcacc aagctggcag
    35641 aagtcacttc tcgttttccg ggcgtagtta aaagtgtaga ttataaatat atgtgttccg
    35701 aaccgggtca aaccttgatc aaggaagcta taaaggaaaa tagactcacc ggagttgtgg
    35761 tagccgcctg ttcaccccga atgcatgaat ctacttttcg taagacttgt tctgaagccg
    35821 gattgaatcc ttttttatgc gaaatagcaa accttaggga gcattgttca tgggtacacg
    35881 aaaaaagtga agaagcaacc aacaaggcta ttgatatagt tcgtgcactg gtagaaaaag
    35941 tcaaactaaa cgaaccactc tatcctataa aagttcctgt taccaaaaca gctcttgtga
    36001 ttggaggtgg tgttgctgga atccaggcag ctctggatat cgccaatggt ggtcataagg
    36061 ttattttagt cgaaaaaaat ccatctatcg gtgggcatat gtcacaattg tctgaaacat
    36121 ttcccacact cgattgctct caatgtatat taacccctcg aatggttgaa ttagctcagc
    36181 atcccaatat cacgctatat tcatatgctg aattggaaag cctcgatgga tttatcggaa
    36241 atttcaaagt taaaatccgt aagaaagcaa aaagcattga cgagaattta tgtacgggat
    36301 gtggactttg tactacgaaa tgtcccgtaa aaaaaatccc caatgaattt gacgaaaatc
    36361 taagttacag aaaagccatt tatgttcctt ttccacaagc cgtgcccaac aaacctgtga
    36421 ttgataggga aaactgcact tattatttga aagggaaatg caagatatgc gaaagatttt
    36481 gccctacaca ggctattcgc ttcgaccaac aagatgaaat tatcgaagtt gaggcaggtg
    36541 ctattgtttt agccacagga tttgatatta agagagcaga tttcttcccc gaatatggct
    36601 ttggcaaata taaggacgtt atcgatggat tgcaatttga acgtattgcc tcagcatcgg
    36661 gacctacatt gggcgaaata cgcagactat cggatggaaa aattcctaag aaggtggttt
    36721 ttattgcatg tgccggttcc cgtgaccctg ccaagggtat agagtactgt tccaaaattt
    36781 gttgcatgta tacggctaaa catgccatcc tgtataaaca caaaattcac gatggtcaag
    36841 cgtatgtatt ttacatggat atccgtgcag gtggaaagaa ctatgatgag tttacccgaa
    36901 gagctataga agaagaaggg gcaaaatata tcagaggcag ggtttcgaga atatatgaac
    36961 gtgacggaaa gttaatcgtt gtgggtgctg atacactgat gaatgcccgc cccgtggtaa
    37021 tcgaagcaga tatggtggta ttggctacgg caggcgttgc tagcccaggt tctgaagaat
    37081 tggcccaaaa actccatgtt gcatacgacc agtataagtt ttttaccgaa gcacatccaa
    37141 aattacgacc ggtagaaacc aatacagcag gaatattttt agccggtgcc tgtcaaagca
    37201 ttaaggatat tcctgaatca gtatcacaag catcaggagc cgctgccaaa gtttgtgggt
    37261 tattctcgca aaatgagctt actcgagatc cattgattgc cgtggttaat agaacggcac
    37321 ctcccttgta ttctacctgc gtcggctgtt tcttatgtca gagtgtatgc ccctatcagg
    37381 ctatagaaaa agaagaaatc aaggctaagg acggaagcgt aattaaaatt atagcaaaaa
    37441 ttaatcccgg tttgtgtcag gggtgtggtt catgtgtggc actttgccgt accaagtcta
    37501 ttgacctctt gggatatacc aatagacaaa tttatgcaga agttgatgca attttaaata
    37561 cattataaca tggacgaatt cgaaccaaaa atcgtagcct ttgtatgtaa ttggtgtact
    37621 tatgcaggcg cagacctcac aggaacaagc agaataaaat atgctccgaa cgtaaggatt
    37681 atacgattcc catgcacagg aagaattgat tttatgttca ttctcaaagc atttgcccga
    37741 ggagccgatg gaattattgt ttctggatgt catccaaacg attgtcatta tacatcaggg
    37801 aatttccatg ctcgaagaag atggatagta ttcagaagtt tactcgattt tttaggtatc
    37861 gacgttagaa gaataacttt ttcatgggtt tcggccgccg aaggtgcaaa atgggccgac
    37921 ctggtaaatc aaactactgc cgaaatccgt aaattaggac cctataccag ttaccataaa
    37981 atatctaaac cagaagagga agtaatctat ggatagagta aaacaaaaag caagagatct
    38041 gcttgattcg aataaggttc aagtggtgat tggctatgaa gaaggtacag acggaacggc
    38101 aagaccggtt tttatcatgg attcaaaaaa tatagataaa ctcattttca acgaccgttg
    38161 ctatcataat cttgctgttt atttaaccaa ggttgaagtc gagaagattg gaagagtggc
    38221 tgtggaggct tcattgaata cacttaaagg tattgtaaaa ttagcatctg aaggtcaatt
    38281 tgtgttcgaa gacatagata ttttgggtgt aaaccgaaat ggcgaattat acgaatttgc
    38341 cagtttggat gaggtgatag actatatcga tcagcatcct gttgaataca gtcccgaaga
    38401 aatatccctt cttcggaaga tcgaatctat gactagggaa gaacgttggg aatactggca
    38461 agaagagttt tcgcgttgca ttcgatgtta tgcatgtcgt gcagcatgcc caaattgtta
    38521 ttgcgatgct tgcatcgtgg atattaacca gccacaatgg atttctccta ctcccgacgc
    38581 cttgggaaat ttagaatggc atatcatcag agcaatgcac ttagccggtc gttgtgtcaa
    38641 ttgtggtgcc tgtgccaaag catgccccgt tgatatccca ttaaacatct taaacctcaa
    38701 attagcagag gaaattagta agaactttgg aatatctgag gaatttttgc taacaaatta
    38761 tcctttagct atttatgatc caaacgataa agaaaatttt attaaataag gctatgacac
    38821 cacaaaataa attattgaaa aaagaatgcc tccccacgtt tgttgaaacc ttggtcaaag
    38881 gtggaaaatt gttgtatgcc cctacaaaag aaaatgaaag gattgaattt aaactcatag
    38941 aaaatttcag cgagatatat ctcgattata tccaaaccac taaatctatt aaaagtattg
    39001 tattcccgcc aatagagaaa ttattcgaat acgtagccga taaggaacat gtcgaaatca
    39061 aggacgtgaa tttgaatact atccccgaaa ccatcgtatt tggcgcacac ccttgtgatg
    39121 ctgcgtcatt caaggtactg aatgccgttt ttacatggga ttgtaatgat gaatttttcc
    39181 ttacccgcaa ggataaaact acagttatag ggctgagttg taaaaatcat gacgattatt
    39241 gtttttgtac atcggtggat ttgaatcctg gttcgaccga aggcagcgat attttgctga
    39301 cgagaatcga aaacgataat tacctggccg aaattatgac tgaaaaaggt gaagcagtat
    39361 ataaacttgt aaaaaatttg ttcgaacctg ctccccaagt ggacaaaaat gcctttgtaa
    39421 cccaattgcc caaacttttc gatttgaatg atctacgagg aaaattgtcg ttcgaaggtc
    39481 tttcatggat gcaacagtca ttacgttgtc ttagctgtgg aacatgtgct tatgtgtgcc
    39541 ctacctgctc ttgtttcgat attcaagata taggcaatca aaccaaaggc aaaagggtta
    39601 gaatatggga tacctgtgct ttgcctcaat ttaccctgca tacgtcgggg cataatccac
    39661 gccattttca aaacgaacgt tggaggcaac gtattatgca taaattttct tatgagccag
    39721 aacggttaca tgtttacggc tgcgttggtt gtggccgttg ttcaagagcc tgtccggtaa
    39781 acatcaatat tttggaaaac ctcattgaaa ttagtggagg aaacaaataa tgcgcaacga
    39841 caatatttat aaaccttgtt tgatgagaat tgaaaaggtt accgatgagg cacccgatgt
    39901 aaagacgttt cgactgaaat ttatcgacga tgtacaagcc agaaattttg atttcaaagc
    39961 cggtcaattt ggcgaatact ctgtcatggg cgaaggcgaa tcgacttttt gtattgcttc
    40021 tccacccacg cgaaaaggtt atatagaatg tacgttcaga aaaacaggaa gggttacatc
    40081 ggcattggca aacaaggaag aaggagaaat aattggcttc agagggcctt atggaaacag
    40141 attccccatt gaagaatgga aaggtaaaaa cctcttgttc gttgcaggcg gtattgcctt
    40201 gcccccaatg agatgtgtca tctggaattg cctcgatttg cgggaagatt ataaagacat
    40261 tactatcgta tatggggcaa aaactgtcga tgatttgata tataaaaatg aactcgaaga
    40321 atggaacgaa cgccccgatg taactctcgt aactacggtt gacccgggag gtgaaacacc
    40381 cgcttggaaa ggacaaatag gatttgtgcc cactatactc gaaaaaatgg aactcaaagc
    40441 cgaaaatacg attgccgtgg tttgtggacc ccctataatg ataaagttta ctttccctgt
    40501 tttggtaaaa gcagaattta aagaagccga tatttttact accctcgaaa atcgtatgaa
    40561 atgcggtttc ggaaagtgtg gaagatgcaa tgtcggtagc gtttacgtct gtaaagacgg
    40621 acccgtgttt acttatgcac aattgaagaa attgccggct gaatattgat cgcacgtctt
    40681 tgatgcagat ttcaatacgg tcatctgctg caccagcgga tataaataag taaatgataa
    40741 tcttctatga acacaaatac agttgaatca gtatattata agaattctaa tggcatattt
    40801 cctcaataga ttataaataa aagtataaaa aatgcccata catttatgct cgaggaggaa
    40861 tgaattaccc cttggatgaa gctttatgaa atagaaaaaa cctcagagaa agattctgga
    40921 aaatttggaa taggtcatgg aagttcaaca taactcaatc cccatatccc gcacactatg
    40981 tcagtttttc attcgctaat tcccaaactc caaccatcgc ttactattgg ccatagcata
    41041 tctcgtttat ctcaatattt tctggatttt cttcctctac tttacggttc tattgtcgta
    41101 cccgagacag gacttgaacc tgcacatccg taaggatact agtccctgaa actagcgcgt
    41161 ctaccaattc cgccactcgg gtgaaaatat gacttgttaa caacggaatg gtgcccagga
    41221 caagactcga acttgcacgc ccgaacggac actaccccct caaagtagcg tgtctaccaa
    41281 ttccaccacc tgggctttaa gtactgcaaa agtacaataa ttttgtcttt tcctaagttc
    41341 tcaagaaaaa atttttattt cttctccttt tatttcgata tcgagtagtc gaattcaaag
    41401 taatgatata caaacaattc aatcttagta ctctttgaat gaatgaattc ctgattcgca
    41461 aaatcttggt caaactaata aaatcatcag cgaatcattt tgatggaata tttatttatg
    41521 aatcgagcta aagaggttat tttctctttg attttaattt attggcttta gcaactggct
    41581 aacatcacta ccgggggcaa attcgcacgg aagattgcca aattgaaaaa ggcgcatcgc
    41641 tctcgaacct ttcaaaacca ttttatcgtc gattacttcg agtaggctag cctctcttaa
    41701 gcctactaca ggaattcggg gatgaagaat tataaattct tcaattcggg cttcacgagt
    41761 ttcgcctcca tgtccttctg gatttttatc gagataatgc gggttgattt gaaaaggcac
    41821 taatcccatg cattcaaaat cagaaggttg aataatcggc atatcgttag tagtcattaa
    41881 gctggggcaa gctacgttgg atcctgcact ccagcccaaa taaggaattc catgcaaggc
    41941 ttgttctcta atagccgaca tcagcccccg tttctgcaag caatacacca gatgaaaggt
    42001 gttgccgccg cctattgcaa tggcttcggc ctccatcacc gcccgtatcg gatgagattg
    42061 acgatggata ctataaattt cgaaccccaa agatttaaac acttcttgca ctcttttttc
    42121 atagacatcg taagattctt tgagaccgag aggagatagc gaaattcctg cataaggcac
    42181 gaaaaggact ttcttcacac ggaatttgtt taaaaaatct gaaatgaaag gcttgggcca
    42241 gtgtagatat tcttctccgt aattggttga attacttatt aatagtagct tcatcatata
    42301 gaatttttaa tcggaatgtc aaaagatgta agttgttcta aaggtaagca tattttcttt
    42361 tagagcattt ttaccgaaaa aggcaagcct tgtggagagg tcgaattgcc aattcttcga
    42421 cttataactt gcacctaaac ataatgaatg gatgaaagcg tcggccccca tggcttgaaa
    42481 aattatttta ttcaatttct cgtcgggttc gggcaagatg ttttcggctc tatcactcca
    42541 accataaaaa taatcatacc cgaccattat tttgagttta ttcaatttat atacatatcc
    42601 cccttgagag gtaaaatatg gatgcagata gatggtggcc tgaatggcat tgtatcgatt
    42661 gttggtgagt attaaagccg tatcgccttc gaaaattgca ccagagcgag gaaaacggaa
    42721 aaggaattca ttatggatga acatctgcga atttaggctt aatttgtgca tgaaggctgc
    42781 ttgaatccct aagttccaat atccatcatt gcctatggga ggtttattgc tgatgtacga
    42841 agttcttgtt tctcttagtg gcaatcctgt acttatttct aggaaaacag atgtttcagt
    42901 aaaatgatat aaatattcag ctttcaacct tatttcagaa acttcggcat ttcgatacga
    42961 tcgcatttcc tgagaattga cagtcttttt aacctggctc tcgtaccatg gcaacaaaag
    43021 accaagtgag aaatttttat atagaggaac ttttgcattc ataaaaaacc ctttacgaat
    43081 caattcgtcg aatggctgtc gacttaggat gttaccctta ccttcgaaag cactgttggc
    43141 ataagaataa aaggcagcta ttctgatgtt ttggcagttc gtggtatctt tagtgtaaaa
    43201 tccattccag aatgggcttc ccattccagc cggaattcca aaagactggc catgaatggt
    43261 gtatatgtcg tttccaaaaa tcaataaaac cagcatgata tatctttgac aaattctccg
    43321 acatggagta agttttttca taatgtttta tctttcgatt gtcaaaaata gtaaggctaa
    43381 attcaaggtc gagttctgtg aaaaatcaga atatggctct ccatccgagc ttctccttta
    43441 aataggcttt gcgtaaggtt ttataccata ctctatcggg aagcatttga tcgagatatt
    43501 caagtgaagc ttctttttta gctggtattt tgtctattct ggctaaatgg tggccgaggt
    43561 tttcttgcca tgtttcgaac caatcagcgc ttaatcctga ttgacgaaat ttttctatta
    43621 gattcaatat gtcactccag cggttttctt ctttaaggtt ttcaaaataa taattaatta
    43681 ttttttcgga gggatgaagc tcaaccagcc agatggtatc gggaagtgga aattctctgg
    43741 agatagaaga gtcggagcta ataagggaat acaattttaa gcggagtttt tcgagtgaat
    43801 cggccagaag tgtttcgtat gtggtggtta attgctctaa cagccgatta tattcaatca
    43861 gtgaatgata ttgatggagt aatccggcaa tacctgaatc atcgagcgga caatcagcaa
    43921 ctgaagtttt tatttgcagg gcagaatcgg ctattgaatc acccattaaa taatctcctt
    43981 cctgaaaggc cgaacgtact ttgtattcga aaagctctaa gcgatatcgg gcaaaacggc
    44041 attcttgaat ctcgatacgc tccgccattt cattcaacct ctcgtttatt tcgctgcaat
    44101 cctgtaaatt ccattgagga atcattttat tggcattcag ccataattga tgtgcctctt
    44161 cgatcttgaa atcccaaagt aagcttccta ctgcagccat cattctctcg aattgaagtt
    44221 tccctaattc gagtttcttt ttacagatga gggaatcatc cgcaaggttt tcagcatatc
    44281 gtaaaagaat tatggaagtg gcattcaaca gggaatcgag aggaagttca ttcgaaattt
    44341 gaatgagaga atccatgaca acataaagag aggctttttg ataaaggtga aatgcagagt
    44401 cttcatcagg aatagactct attgaagtag catttgtttt tttcatcgtg agtgcttcat
    44461 cgagtaatct tttcccttcg atataatttc ccaattgcat ggagcttaat gcggagatta
    44521 ctttttgtcg atatatttcc tgaagcgttt tggatagtgt ttgtttaatt tcttgctgcc
    44581 gtaaataaat gctttgacga cctgcatatt ccattgcttt ttctaaaaaa ggcaaagcct
    44641 gttcgaactt cccttcttta ttcaatatta tagctttatc gaggcaaacg tcggaaaaaa
    44701 tttcatatac tgatttagac ggttggctcc ctaataggat ttcacggttt tcagattggt
    44761 actccagagc ttttttcaga tagaattcgc ccatgacaat atttccattt agaattccat
    44821 cgcgggccac cttcagatag gtattgtata accctcttac cgatacttta atttgagact
    44881 cgacggcctg ggtttcatct tccaaatcag cagcctgagc tattttcagc gcattattca
    44941 gaataatatt ggcttggata aatagagttt gcttattcaa tgcctcgcct tcttgtagca
    45001 ttttttttgt caattgtcgg cttagatttt ttagaaattc atcctcgttt gtataaccca
    45061 ttttcaagag agcagaatga agcagctcat gcgccatttc tatccatttc ccatgtggat
    45121 cgaggcgggc aacctcagcg ataactccgt tgttcagcgg attagaaaat tcttcgtgcc
    45181 gaatccatga aatccagaaa ccaaaaatac ggtctgacaa aggggttaaa tcgttgggcg
    45241 ataggggatc ggtatcgagt aatcgttcga aagccagtct atggcggtag atttcaatat
    45301 cgatagcttt cagcgagtta tacaagttgc atggatcggt tttctccaag ggaagaatta
    45361 aggcagagaa aatggaatca agagattctt tcacacggat tccttcgatg agctcggcta
    45421 aaatatcaga aatacgagga tcgtgaggat tgtatccctg gcggaggaca ttttcgaaat
    45481 aaaggctcga ggcacaaagc ccacggtaag tctttatttc atccatcacc cttttcagct
    45541 gcctttctat ctcttcatcg agattagcat ttcgaaaatg cagacttagg ctatctgtcg
    45601 aaagattaac cttaggcgaa aactctaaaa ttactgtatc atgctccagt ttgacctctt
    45661 gggtgtaaat tattccatga aaagaatgct gaagataggc ttggaggtac aatggcaaaa
    45721 gaaaatcaga aagatcataa ccaaggtatt ttgtcggacc ggtaatatga tggagaacga
    45781 ctttccattt accaaccgaa gaatcattta tttgtttttg atgatataaa acgtaaccca
    45841 gttttatttt acgatagcta agtccatttt cgaatgccag ttcttcgagg attcgattgg
    45901 ttgcctgaat gtttcgggaa gtatcctttt tccatttcag ccagacagtg tcctgagttc
    45961 cggcaaacga agcctgagca aggaagagaa agaggatgaa acagataaat ggagaggttt
    46021 tcattgatag aaagattgaa tcaataaacg agattataag ttgcttcgct aaagttccca
    46081 acgttgtcgg ttaccctgat atggagtaaa ttctcacctg tcgaaagcat ttcgtcggga
    46141 aaataagtga gcaaattatt tttggcatca tattccagta agatccattc cccattgatg
    46201 ctgcctatgt atgaatcaat gcctgagagg tcgtcggata ttctcacatt taagcgaggt
    46261 aaatttttta tgttttgtcc atttttaaaa ttcagatttt tgataaccgg aggcagtgta
    46321 tcggctaaaa ctgcataagt accgaatgaa cgtatggaag ccttaaggga actgccttcc
    46381 catttgctaa tcatggcaga aggtttgttt ccttggttta ctttcacaat aatggcttta
    46441 tgatgaagac ggcttggtaa gcggttgggg cgaatggaaa tttttgctga agaatgaata
    46501 ggcgttgtag gtgtgtggat gcggtgaaaa tcggagaaaa atgctgatcc tgaggttatt
    46561 cgtttataag aaaaatttac ggtgtcgtaa aaggctccaa tgggcgccca gaaacagaaa
    46621 tccacggtgt catacgaagc ttcgcgatct cccgaaaatt gaatgtgaga tgggacgaat
    46681 ggacgaatgg gagcataaac ctttggtatt cccttcacta cgaaacgaag aatggattcg
    46741 ttgccataat gatcgctaac cacatattta agatgataga tattatcggg caaaaatgca
    46801 tatgagccat tatttaacaa gattctatag atattttgaa gacggctgta aggagcttta
    46861 cgcgtttgaa tgatccattg cttgttgaga acgatttcgg aataatcgca taaagcattg
    46921 atatatcgcc ctttatcgaa atacaaacga ttgaattcga tagcgaaaac tggaatagaa
    46981 tcgatgaaca attcaactga tttcacgccg ttatgattag gttcgtcatt cagaagatcg
    47041 tgggttttaa ttccgaacca agcatttcca gcaataaaca aagtatcttt gttacgaagg
    47101 cgataatagg gcccccaacc tgtgaggttt agctttatgg ggtgaggaga accattgact
    47161 agtgaacca
//
LOCUS       DAOORU010000004        46466 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_867_length_46466_cov_6.177307, whole genome shotgun sequence.
ACCESSION   DAOORU010000004 DAOORU010000000
VERSION     DAOORU010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 46466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46466
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_867_length_46466_cov_6.177307"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(25..2187)
                     /locus_tag="PLF20_00730"
     CDS             complement(25..2187)
                     /locus_tag="PLF20_00730"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOX79567.1"
                     /translation="MNYTSLSLSKKKLLVLFLLLNYVLISQAQEKHFTAEDASYNNPA
                     LYPKGLSNLSWIPETDFYAFTNEKALIKKEATKETSDTLIFLQQFNQLLVQNNEKAGK
                     GFPRMTWINKNEFRFQKGNKFYLCNPESQRIVLLTQIPENAGNVDISESSQHIAYTLD
                     NNLYINLRGEQRIISDEKNSGILYGSERVYRNEWGISKGTFWSPDGTKLAFYRMDETM
                     VTDYPIIDITSRPAKVNNIKYPMAGMPIHHVTVGVYNILTQKTVYLNTPEPSAHYLTN
                     ITWSPDNKSIYIAVLNREQNHLWLNCYDVSTGNLKRILFEETSPRYVEPENGPWFMPA
                     NPAQFVWQSERDGYNHLYLYDTSGNLIKQLTKGNWEVQEISGYSQKLHKFIILSTKDS
                     PLESNIFTVDIRNNEVRRINEEGGSHSVKFNSYGDCFLDIYSNSEVPLKIRLNKLNAK
                     IDKIIFTAENPLKDYKLGETTVFTIKNDDNDDLYCRMITPPDFDTSKIYPVIVYVYGG
                     PHNQLIRNSWLSGAGIYLNYLSQRGYIVFSLDNRGSANRGFEFESVIHRHVGMYEVED
                     QICGINYLKQLHYVDTTRIGLDGWSYGGFMVINLMLQHPQTFKAGCAGGPVCDWKYYE
                     AMYGERYMDTPEENPEGYQNSSLLSKANCLNRRLLVIHCTTDPVVVWQNSIDFVQKCI
                     EKGILLDYFIYPGHDHNVAGKDRAHLIKKIEDYFERNL"
     gene            complement(2201..3244)
                     /locus_tag="PLF20_00735"
     CDS             complement(2201..3244)
                     /locus_tag="PLF20_00735"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOX79568.1"
                     /translation="MKKLLTVIIVFLSLITATSCSKSSSKKSSTGGTLEVLVVTDSHE
                     QWNGKIGDTIRSLFARYIPCLPMPEESFTPLPILSKTLYENDMYKAHHNIFIVSIDPK
                     LTKPSINLERNYWAEPQLIIHFSCPSDTSFFCLFNQYYPSLYELYREVEKDRIQKLFT
                     SNPAKEIITKLQSKYGIDMLIPGGFTVAREEENFIWIRQTIHRRKQDTETGFLVYYRP
                     YTDTAQFNGKNIIRTRDSITRKYIPGAVEGSYMATSVDVIPPQFKRTSKFSTGFAVET
                     RGLWKVVNDFMGGPFISYTFADTKRQRIVTVEGYLYHPNSEQRKYMLQIESLLNTAKV
                     YDEQEQQNVPTKK"
     gene            complement(3252..5012)
                     /locus_tag="PLF20_00740"
     CDS             complement(3252..5012)
                     /locus_tag="PLF20_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOX79569.1"
                     /translation="MKGIRKILILFILLSTLEGQSQKVLVHDTIHTSDSGIFFLPYKK
                     VFNPEVDLIEDSPILRALDSLANIKFFQDYYFTADASLTNVYQFPGDFIPVYSDSVYQ
                     QRIENMKQNSPIDYTFNCYVKDYIELYAVKKRQLTQRLLGLSYIYFPLFEGMLDKYEL
                     PLELKYLAVIESALNPIAGSRAGAKGLWQFMYGTGKVYGLKATSLVDDRFDPYLSTDA
                     ACRHLKDLYDLYEDWFLVLAAYNSGVGNVNKAIRRAGGTKNYWAIWPYLPAETRGYVP
                     AFIAASYIMQYAPEHNLYPLHPGILYNGIDTVVVREPVSFDQISEVLGVPIEDIKFLN
                     PSYKLGIIPVAKDQKYYLQLPREFIGDFINNEYTIYNYTTTKGIEKEKLLKQIKNIHE
                     QQIHIVRSGESLSSIARKYHCSVSDIKKWNNLKSNTVKAGKRLIIYSGTSSIASTSSS
                     NNNQTTDTKPEISNSSTNKEQHPNYHIVKRGETLSQISIKYNCTINELISWNNLKGYN
                     IQVNQRLLIHPPAAKNDSASSEINKQVKYRYYTVKEGDTLWDIAQQFDGVTVDEIKKL
                     NNLRSNRISVGQKLKIKVIN"
     gene            5679..7730
                     /locus_tag="PLF20_00745"
     CDS             5679..7730
                     /locus_tag="PLF20_00745"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOX79570.1"
                     /translation="MRSKSFILLNVLIVVAALCHFTFAQEPLQCTSIVLTHWMTPDEF
                     LRRSEIGRGFTPTPPPPAPVTNLAEFNYNEGVLIRYPFGIPMALIKKMSEVGIVITIV
                     ANHSAQNTVYNQYVNNGVNLTNCSFLIAPTDSYWTRDYGPWFVINGENKVGIVDFPYN
                     RPRPSDDDIPVKMASFMDVPLYGMNIIHTGGNYMCDGMEAAASTNLVYSENPSLSPSQ
                     IHQMMNDYLGIINYHVRPDPNGTYIDHIDCWAKFLDVDKILVRQVPSSHPQYNLIEQA
                     AAYWQSAISSYGTPYQVFRVNTPNNQPYTNSFILKDHVFVPFTGNTLYDNEALQAYQN
                     AMPGYQIIGVYQNPSTPWESTDALHCRTHEVADKYMLFIKHLPIVHTQPCDEDYLITT
                     YIKSLCDSAIIADSTRVFYSIDNGPYQIIPLSYQSNDQYTALIPRQPAGSSIRYFLQA
                     AEKSGRNMYHPFIGVTDPHNFMVGEGIHPHITLSTHSVDTSSNPGQVVNFPISITNVG
                     YADLAWILQIDSASSSWLYSSDIMGTLSPGSTQDITLTLDATFLSPALYESKISIQSN
                     DPDQPVDNILVHFNVFSGIGIHNEIDWLADCKIGPNPFSNQLLIEFRLNQSIPVIIEI
                     YNSSGYIVKTISKGYLPSGVNHFIWNTPESSFISLPDGLYLVKISTDKSHKLIKVIHK
                     N"
     gene            complement(7967..9907)
                     /gene="dnaG"
                     /locus_tag="PLF20_00750"
     CDS             complement(7967..9907)
                     /gene="dnaG"
                     /locus_tag="PLF20_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOX79571.1"
                     /translation="MIPESTVSLILETARIEEVIGEYVHLRRRGANLVGLCPFHSEKT
                     PSFTVSPLKGFYKCFGCGAAGNVVKFLMEYEHYTYIEALRYLARKYNIEIEEREPTEE
                     EIQKANEKESLYSVNAMAAEFFKTQLWETPEGRNIGLSYFEERGFQHPIIQRFQLGYS
                     PMQKDALYIYALSKGFDPDYIEKAGLIIKHGNEYIDRFFNRVIFPIHNQSGRIIGFGG
                     RILTNDKLFPKYINTPETEIYHKSNVLYGFYQARKQIADANNCYLVEGYTDVISMNQI
                     GVSNVVASSGTSLTPGQIHLIKRYTPNVTILYDGDTAGIKASFRGIDMILQEGMNVRI
                     VLFPKDHDPDSFARSHRTEEVVSFITEKAQHFIGFKTNVLLDETQNDPIKRSALIKEI
                     LDSIALVPSSIARSEFIKYTSETLNIPEQTLLYELNKILSRKYKNFKPEPQPLSPPNL
                     QIDSNQEIAEERNITHQEYNIIRLLLNHGEKNFEFQFDDESGQQQFMKVNTSHFIVSE
                     LIEDDYEFSSPVLQKLYQEYKAAFEKNIILPEKYFINHPDEEIANIAMNFLASNYQIS
                     ENWFKQKGIFTPKEIDKLKDLVINSVYNLRLRRIELEINKVQQELQRADWEEAWNLLK
                     QLNDLNVSKSQISKKLGRIILK"
     gene            complement(10034..12148)
                     /locus_tag="PLF20_00755"
     CDS             complement(10034..12148)
                     /locus_tag="PLF20_00755"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX79572.1"
                     /translation="MAILGNIRKRSGVAIIFVGVAILAFVLGDIGKSTWRNPTAIGVV
                     AGEKISYIDFEKKVEENLGYTKQSTQKEALSTEEIYSVRQNTWNQLVNQIIMGKAFKE
                     TGLSISQDELTDLIQGPNPHKYILQNFQNPQTGLLDRELLINFLQTLDQREPEIQKQM
                     ENLIEAIREDRLNTKFNVLIGKAYYMPRPLVLKDYQLKNVKASFNFVAQSFTLIPDSL
                     VKITDKDLLAYYEKHKQYYEQEESRDIDYVVFDVLPSAEDYAMAENEIRRLYEEMSTT
                     TDIVNFVNFNSDEKYDSSFKKKGTLPVQIDSIMFNSPIGTMAGPWMDNNVYYIAKLMD
                     VQERPDSLKASHILISYKGAYGASQDIKRTREEAKKLADSLMAVLKADKKKLAEIAAK
                     FSDDPSVTQNSGDLGWFADGQMIYPFNQAVLKTKVGDFTTVESAFGYHVIEVTGKTTP
                     IKKVRVAILKKVINPSTRTQQKIYAQASTFAAQCKTRDDFDKKVQELGLNKRSKDYLN
                     AMDMGLPGIDDSRDIVRWAFSKEVKLGAISDVKEFGNKFVVATLKKIHPKGIAPLEDI
                     KEGIEASVKKEKKVQMLAAKMKEVQTKDLYELAIRLNAKVDTASDITFYTYNIPGIGR
                     EPNLIGTVFSLNPNEVSDPIEGINNAFVAKVKSFTDAPAKSDFTFETKQLENNFFARS
                     SSGTYKALEKLAEIEDNRVLFY"
     gene            complement(12288..13556)
                     /locus_tag="PLF20_00760"
     CDS             complement(12288..13556)
                     /locus_tag="PLF20_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOX79573.1"
                     /translation="MGNNYIIVVISLLFSALFSGSEMAFFTANRLKIALAKNNKSWSA
                     VILSKFVENPSAFIATMLLGNTITLVVYSLAMEPILSHEIFPRIGLGNIPSTSLILFL
                     QTLISTLIVLVFAEFLPKSLFKIQPNVILNFLAIPLYIIYLILFPIVYLVNKMSHIFI
                     VKILKQELPSQDTSITWVDLDKFIREFTQSTGNKHIEQEAHILQAALDFKETKLRECM
                     VPRTELVAVEKNESIPILINKFVETGHSKILVYNSTIDDIIGYVHSFDLFNKPTDLQS
                     IIRKVEYFPETMTADKALKKLTHDKISVAIILDEFGGTSGMVTLEDLIEEIFGEINDE
                     YDSNSVNDRKINEKEYIFSGRAEIDYINNKYNLGLPESDEYETIAGLIIHYYEGIPSN
                     NEQITIEPYCFQILQSTGKRIEKVRMTLKN"
     gene            complement(13582..14199)
                     /gene="lptC"
                     /locus_tag="PLF20_00765"
     CDS             complement(13582..14199)
                     /gene="lptC"
                     /locus_tag="PLF20_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOX79574.1"
                     /translation="MKLLRLGLPGKVKMNSSVFYEYRVSVFFITAIAILMVMAVSCKN
                     DMKRIQELTAIDTLPDEVIKNIQLRYVDSGRVTSLLKSPLLYTYSTPEAKTIFPQGLE
                     VYFYDKQMRITASLTSRFGINYMNEKRIEVKNNVVVTNMEKGEKLETEELAWDQRKGK
                     IFTEANIKITTPDKIIFGRGLESDDNFRRRIIRNVSGEILVEDEQ"
     gene            complement(14168..15541)
                     /locus_tag="PLF20_00770"
     CDS             complement(14168..15541)
                     /locus_tag="PLF20_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX79575.1"
                     /translation="MKTNKTILYFIVSLLLSLGSIRVFGQVSACNEKAKYGQDSVECV
                     KNLSLYREPVKQKNYKDAYRHWRWVYQNCPCASQFTFVDGIKIMEYRLENEKDPLRKN
                     QIIDSLLEIYDARIKYFPINPSNGQSQVGSILTRKALDCFTYKPDKPEEAFQIIKRAI
                     EIDGAEVLPSGFQVYMEAAIEMVKKELADKTLVVDVYDQLSDLMDQAMDNAHTDSVAI
                     KKYYLIKNNIEVLFEPFATCEDLVNIYHSKFNQNPNDTTLLKKITKVLDKKQCTDSEL
                     FFAATENLHKIEPSANSAYLMALKYIKEEKWDVAKKYLEESLQLYKPDELNKKARIYH
                     LLGQVYLAQKSFPAARSMAQKILEINPNNGEAYIIIGDAYALSASQCGDNELTKRAAY
                     WAAVDKYMQARRLDPRVEEIATKRIATYSSYFPSIETIFFYGLKQGESYRVECWINET
                     TTVRPAR"
     gene            complement(15560..16849)
                     /locus_tag="PLF20_00775"
     CDS             complement(15560..16849)
                     /locus_tag="PLF20_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79576.1"
                     /translation="MSVNRKILFVLPLIMMACLSKKSLSQSSITSPYSHYGIGDLSSI
                     SNTRNRTMGSIGYAQRGFYNVNPINPASYTSYDSMSFVFEGGMKSSFVTLKTSGISES
                     ATSASLDFLFFGFPVSRRIGVSFGTMPFSTVGYKILQKQTDPMLGEVNYYYQGDGGLN
                     QYYIGAAFKLHKNFSIGFNAIYLAGNLERSRLLHFPDSVAILNTRIKDNIHISDISYS
                     AGIQYHRTLSKGNLFNLGATVSYGNKLNTNTDKLSETLFGGINGGYELYKDTIEYISD
                     IEGDLKLPLHFGGGFLYEIPEKLCFGADFMYSFWEDYTISGKSDSLKNSYRINAGVEW
                     IPDNRPISKYWQKIRYRFGINYNDTYLRLNGTNINEFTASIGFGLPLRRQAASINFGA
                     EFGNRGTIHNQLIKENNFKIYIGISITERWFVKRKYN"
     gene            complement(16839..17573)
                     /locus_tag="PLF20_00780"
     CDS             complement(16839..17573)
                     /locus_tag="PLF20_00780"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOX79577.1"
                     /translation="MLNIAIDIGNSMTKVGYFENDQLVHFEKVRKLTADRLKGITNRY
                     FSNSPEAVILSNVKEHDIGLYRFLSQTYRFYEVGFNTQYPIKIAYKTPHTLGNDRIAA
                     AAAAYSKSSGAPALVITCGSCIIYDFVDENGNYLGGAISPGLRMRLKALHNFSAKLPL
                     IKPRFIDSNLGVDTETSILSGVINGMTYEVDGFIDEFRTKNIDLSVFLSGGDMLYFEG
                     KFKNSIFAVPNIVLEGLNKILLFNER"
     gene            17672..17744
                     /locus_tag="PLF20_00785"
     tRNA            17672..17744
                     /locus_tag="PLF20_00785"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17705..17707,aa:Phe,seq:gaa)
     gene            complement(17878..18117)
                     /locus_tag="PLF20_00790"
     CDS             complement(17878..18117)
                     /locus_tag="PLF20_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOX79578.1"
                     /translation="MKVELISPQKRIFHSDDIILVQLPGIDGLFEILNRHAPMIALLK
                     KGNIKVKDSQNKVTFFRIEKGFVEVKNNVVTIFSQ"
     gene            complement(18123..19628)
                     /gene="atpD"
                     /locus_tag="PLF20_00795"
     CDS             complement(18123..19628)
                     /gene="atpD"
                     /locus_tag="PLF20_00795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOX79579.1"
                     /translation="MKSGIGKISQIIGPVIDVTFKEADELPNIYESLIVKRPDGTDLV
                     LECQQQIGEATIRAIAMDSTDGLMRGLNVIKTGKPISMPVGENIRGRLFNVIGQTIDG
                     LPNIEVKQTYAIHRDPPKFENLTTNNEVLFTGIKVIDLIEPYAKGGKIGLFGGAGVGK
                     TVIIMELINNIAKRYSGLSVFGGVGERTREGNDLLREMISSGVIRYGHEFIDEMEKGG
                     WDLSKVDMEELAKSQATLVFGQMNEPPGARARVTLSGLTLAEYFRDGDDQSGGRDILF
                     FIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGLLQERITSTKRGSITSVQAIY
                     VPADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLESTSRILTPENVGEEHYN
                     TAQNVKRILQRYKELQDIIAILGMDELSEEDRLIVQRARKVQRFLSQPFHVAEQFTGV
                     KGTMVGIEDTIKGFNMIMNGEVDEFPEAAFNLVGTIEEAIEKGKKFVEQSK"
     gene            complement(19677..20333)
                     /locus_tag="PLF20_00800"
     CDS             complement(19677..20333)
                     /locus_tag="PLF20_00800"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HOX79580.1"
                     /translation="MKSKNKAIWLAIGTVALVLFVDQVLKIWIKTHFFLGEEIPVFDN
                     WFFLHFVENEGMAFGFSFGGNSGKILLSLLRIVAVLAIGYYLYLLIKRESKPLKIIVF
                     SLIFAGALGNIIDSCFYGLIFTESTYYQIASFTLKNGYAPFLHGRVVDMLYFPLIETQ
                     WPTWFPFIGGQEFQFFRPVFNIADSAITIGVFMLIIFYKNIFEVSAQKEAIHKIKEEY
                     "
     gene            complement(20362..20760)
                     /locus_tag="PLF20_00805"
     CDS             complement(20362..20760)
                     /locus_tag="PLF20_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOX79581.1"
                     /translation="MKKEEAPKERYSDEELEEFRQIIMVKLEKARKDLALLMEAYANL
                     EENGTSDTSPTFKVLEEGNAVLTKEENGKLAARTEKFIKALENALVRIENKTYGICRE
                     TGTLIPKERLRAVPHATLSIDAKLKQKRNK"
     gene            complement(20795..24157)
                     /gene="ileS"
                     /locus_tag="PLF20_00810"
     CDS             complement(20795..24157)
                     /gene="ileS"
                     /locus_tag="PLF20_00810"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOX79582.1"
                     /translation="MSKKYREYKCLDLSKICNEILNVWDQDHTFQKSLEKTKTGEPFI
                     FYEGPPSANGVPGIHHVMARTIKDIVCRYKTLKGYYVERKAGWDTHGLPVEIEVEKKL
                     GITKDDIGNKISIVDYNNECRKEVMKYKNLWDELTIKMGYWVDLEHPYITFDNKYIES
                     VWYLLLQLYHKGLLYKGYTVQPYSPAAGTGLSTHELNQPGCYKMVKDNSVIAQFKIIK
                     DEKSLFLFRLTQYEIFCLAWTTTPWTLPSNTGLAVGENISYSLVETYNPYTGLAEAVI
                     GATQLLPTILDFRNKDLDFDSYKPGDKKIPFRIVAEFLGKELEGIRYEQLLPYAKPKD
                     GDPFRIVLGDFVSTVEGTGIVHIAPSFGADDFKVGKQYNLGYLTLVDKKGKFTSEMGE
                     FAGRYVRPEYEPDGDHSRGPVDIEIIVKLKKENKAFRSEKYEHSYPHCWRTDKPILYY
                     PLDSWFIKTTAMKDKMIEENDKILWKPQSTGTGRFGNWLENLVDWNLSRTRFWGTPLP
                     IWRTKDNQEEICIGSVEQLFLECEKSVESGFMKVNPFKDCKNASTGKIDYNSFDLHRP
                     FVDEIILLSPLGKPMYRESDLIDVWFDSGAMPYAQFHYPFEREDELDLFFPADFIAEG
                     VDQTRGWFYTLHAIAVMIFGKATFKAVVSNGLVLDKEGNKMSKRVGNVVNPFTTIENY
                     GPDATRWYLITNSNPWDNLKFDENGIIDVSRKFFSTLFNTYAFFALYANIDNFQYTEE
                     DMPISQRPELDRWILSTLNSLIEEVDECFNDYDLTKAGRLIASFTLEDLSNWYVRLSR
                     RRFWKGDYTQDKISAYQTLYTCLITIAKLASPIAPFYMDQIYKDLNSITQKEKYDSVH
                     LTDFPVINEKLIDKELEERMKTAQKITSMILSLRKKMNLRVRQPLKKVLIPAINSHTS
                     KLIASIKDIILHETNIKELEVLPEEDTTIVKKVKPDYKKLGPKLGKKMKSVANALENL
                     SQEQIRELEQKQSIQLKIDGDLVLIHLEEVEIITEDIPGWISITETPYTVALDLTLTD
                     DLIDEGYVRDLINRVQNIRKESGFDVTDKIQLKIYHHPTLTKAIEKNYHYICSETLAK
                     ELLFTDQLEQEMIEVDIANEFKVFIKVNRIEN"
     gene            complement(24214..25947)
                     /locus_tag="PLF20_00815"
     CDS             complement(24214..25947)
                     /locus_tag="PLF20_00815"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOX79583.1"
                     /translation="MSLLSTEEVIKAIPILKSKFGVKIANFLIRLNKMDSINQLYDDL
                     VASGLHNRDGLGFLFDQLSVGHSVNPGGLENIPQEGPFVTISNHPFGGMDGMMLAYIV
                     SEIRDDFKLMANFLLYKIEPLREIFLPVNPFEDRKDLTSSIKGLREAYRHVNAGHPLG
                     LFPAGAVSAINFKDFSIEDRPWPHNVKKLLRQLNVPIIPIHFSGYNSLLFYSLGLINP
                     KLRSLRLPAEVLTKSGKVIKVTIGKAIHPKEYINLSLNDFGDILRTKIYSLDYSFSNK
                     IEYRATNLVYNNEKSFTNDSLVQSEINKIEKELILQLDDFQFYFTQSNKILYSVRSFL
                     KSKEKIYYTNKFKNNIATIDELDNESRYAFVWDRKNSTIVYACRIDLGKEIIENHRMK
                     CIFINNVFKLNTEVKSLVNNSILFDELYFANDYRGNNIFYITLWESIFEIFRNLKYHY
                     LISLFHIPYIYQKETTSLFINFLKENFFDNTFSEHIKSKNRSTLPFPKRLSKKAFLDF
                     TGKNFNKLEEFLTDLEPTFEIDPIICQKLYLMHSTILGLNINTKKSSYIDTLFLTDIT
                     MVPENFRQLLE"
     gene            26351..29287
                     /locus_tag="PLF20_00820"
     CDS             26351..29287
                     /locus_tag="PLF20_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79584.1"
                     /translation="MNSKDFFSFFNLVQLSKMPSKFARLAFNQLTLKFKRDIKFIEIK
                     NFIRLKNVKYFTISFVILLVIFIFSFSSFKEPYKRIVNYQTAFSKPDLLQYTITPDNL
                     ICNENDDFVINLKITGNFIPDKIQIIKGIYTDYFIKTSEKEFYYKIHDVHKEISFEIV
                     CKTLNHHKKYLLKVRPKSFFKDLEILVIPPAYLGLSNDTIYDLRELFIPEGSLIHLNL
                     NFNKGDSIFISNELTNYRETFKLDKDYFTKSFYVFDNTNINFKLFCDRIISDSLSIHI
                     NAVKDRPPLIYSELLSNAEYIANFILNINDDYKINKIGYEFIKINYGNLKIIKNFNLF
                     TNWNSSNFSNNIEVNYNEFSEIDSLGIRFFADDYYPYKTNHKAYSPIIILRHKESLSS
                     IISEINNSQENINQIDNSLKNFENLRSKINSINNINQLDNFQKKQLIDEIQSTYDHFR
                     KNSDHFMNQNNLNTDEKTDLKNFLNIIDSIYNNLKNNLDLNNINNLDLNKQINDLKQL
                     VDIMNNKFKNFLDRNIFENIFDRLNELINNQIRNNNNLIYKTDSWKNSDTAYDSKLKE
                     ELNQLIKKHEDIKNEYDKAVKENFINNNLKFQNDSILKSISNEYEFLKQNSSLIDKGL
                     KRINESYNEFQNNLQNEGMVNEDKLIDENALKSLLKSLIYFSKQIEYSSTNSSYYRAL
                     PNDSVFTNFIHFSYNWSSINDSLNRFLKQADIPVSSIANSSLKINKAIKDANKAIKEN
                     NYLNFSKETNVIMSEINLINLSLSELLKRIEENNKNNTMEMAGMCSKPMKKGGNKQQS
                     TNSLSEIIKIQQQLTEKMGQKQMKGENNSGFGNKEINEYINLQYEIRKKLSELLQNDD
                     MQKYSKILESINHDMRESERKLLNKNSNPTEALRRQKNIETRLLEAEKAIREQEFENK
                     RVSKDAGNYIRKWDEVAENEYILKKEKSLDNLKLNEVPLRIFYQNAFLNYQNSSKNVN
                     FTDKSR"
     gene            29678..30100
                     /gene="ybeY"
                     /locus_tag="PLF20_00825"
     CDS             29678..30100
                     /gene="ybeY"
                     /locus_tag="PLF20_00825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOX79585.1"
                     /translation="MISFYSIGFNNPLKKIRTLRCFLIGILNEHSKKDGNINIVFVDD
                     EYLLKINNEFLHHDYYTDTITFPYHLKHSKVIEGDIFISYDRIKENAKLYKCSVQIEL
                     IRVIIHSILHLLEYQDKNGNNIIFKKQEKYLQDFLEQL"
     gene            30142..32019
                     /gene="mnmG"
                     /locus_tag="PLF20_00830"
     CDS             30142..32019
                     /gene="mnmG"
                     /locus_tag="PLF20_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOX79586.1"
                     /translation="MIFDHYDIVVVGAGHAGCEASAAAANLGSKVLLITMNLLTIGQM
                     SCNPSIGGVGKGQITREIDALGGYTALVSDKSMVQFRMLNKSKGPAMWSPRAQNDRLM
                     FHVEWRKVLESIPNVHFLQDQVVNLIVKNRKILGVVTGMGNKIYCKAVIITSGTFLNG
                     KIHIGEKNFSGGRIAETSVSGLTEIINRLEIKSGRLKTGTPPRIDSRSIDFSKLTQQV
                     GDKPPGQMSFREIIDKENFYQLPCYITYTNSFVHEILKKGFDRSPLFTGRIQGVGPRY
                     CPSIEDKIQRFSSKDSHQLFLEPEGRTTVEYYLNGFSSSLPDEIQIEALHRIIGLEKA
                     RIFRPGYAIEYDYFYPSQLNDNLESKIIENLFFAGQVNGTTGYEEAAAQGLMAGINAH
                     LKSHGLKKFTLSRSEAYIGVLIDDLITKELNEPYRLFTSRAEFRTLLRQDNADFRLTP
                     LGYSIGLVSNQQWINLCDKYRTIQKILQFLEIDNILQLYNYNSDKEALPQNNPIINFL
                     VQPGVSIYQTLNNFPNFKQYVEDLHANEEEIEEAEIIIKYYEYIQKEIEVANRIKKFE
                     NLYLSPLIDYSLFPSLTIEAREKLNKIKPSTLGQASRISGVSPSDLSFLLIYLNKNYS
                     S"
     gene            32025..32906
                     /locus_tag="PLF20_00835"
     CDS             32025..32906
                     /locus_tag="PLF20_00835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX79587.1"
                     /translation="MQYVICPICKNEKVNRILVTKDFFLSNEEFEIWECEFCKIRFTQ
                     PIPSEEKIQDYYKKEEYFSHYKNKKSLSYYIYNIARKINLRRKYKFISKDLQLKIGRV
                     LDVGCGSGDFLNYLFSKGWDCAGVEPDNDSRHYLTKMNKFPVYSSLNEIESANSFNLI
                     TFWHVLEHIHHPEDAIQKIINLLNKEGILLIAVPNYDSPDAEFFMSYWAAWDVPRHLF
                     HFNLLSLSKLLKINNLQVIKHSGLYFDVLYISYLSQKYLNKLIPCITGSFIGFRLMLK
                     SKTMNNPSSIIVVAKKQ"
     gene            32903..36559
                     /locus_tag="PLF20_00840"
     CDS             32903..36559
                     /locus_tag="PLF20_00840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX79588.1"
                     /translation="MKKAHYKISRFSSKSIVIGITLLLLAGIFSIIWFNNYLQWKKFQ
                     KTIYNSYNQISNLTYELKNQLDSNNNYANITNKLKQIKNSDELQIIIYKKPDHLVYWH
                     NNQYLINLNELRFAEDGFISNKKGSFLYTCDSTNEFILMVAFPVWIEFAIQNEGFQPE
                     PSSIFKKFGLFSLCPPANEGFPIYNSQGKEIFFVEFNNPVHYYPWKYLLIGTLIIIGF
                     YITVIQLFNLIFKKEYQINIFIILSIFILFCLFYKLGKYFEHEYFISYHNYLVIFNKL
                     ISYSELLFFSILSAIFIKNLCKILENYNKRKIQKYSKIQFSTIIIFLVYIILLIVFYY
                     VIDQIIINSDISFTPAMFFDITYINGVSLISLFVLLYIILYITIKIVNIFQNPIISKY
                     HPYLLIILILLVTISFISKIFIVFTLLISIASIEVVVIIKNSIHFDINLLEKFLYVLI
                     ISLFLILYLIYNLEEKEYSKKQLILMNIESMEQGDPITESLFADIVSEIQYDSLILNL
                     IDNSAYNPVLVENYIHEKYFRGFWNQFHITITICKENERIRITPILEEISCYEYFNHL
                     FDLKPDSIYDTSFIKISDYGHRLNGYTGVISFPHIPFKLFVELLPNPSSNATFLYDLV
                     TSQDLKSRINLENYSFARYFNNKLISFSGSAVFPVDLPKSFIINEKDESSIIEVKSGK
                     FHYSILKSKNKSIYILANEVKRINNFISFLGLFLIIYTIWGIVYFLWNLKSKNFYFNR
                     IGIKFQIVITIILLVSFSIIGYFVYNLFDSNYKNQVKAKLVETIYSVSSEFAYKLIEE
                     PNFLYKDIDLEAFIIKLSNIFFTDINLYDINGKLITSTSPKLFHFRIVEPLINRTAYN
                     VLTEGQATTYLQEEKVGNYNFLSGYIPLRDNDNTIVAYINLPQISRISQQQQQREEYL
                     STLISVFIFLLIFSLLIALFIANIIVKPLNVLSESLSQLRLGESNIKIPIVRNDEIGL
                     LIEKYNNLVDQLESAINELKHKERDLAWKDIARQIAHEIKNPLTPIRLQIQFLSASFK
                     PGDSNWNKKFYEFSNLLLEQIDLLSQLADAFSELGHLSESKQVQVNLIEIVEEAANIV
                     LTGFSGEFIHHFPDYSTIVKADRNQLLRLFINILKNATQAVQDQENKLIEIKYLLDYD
                     HVIVSIYDNGKGMNEEEKKKIFTPYFTTKSFGTGLGLYISKNIIKAHGGEITFESSVE
                     NGTTFYIKLPMFSE"
     gene            36647..37093
                     /gene="dut"
                     /locus_tag="PLF20_00845"
     CDS             36647..37093
                     /gene="dut"
                     /locus_tag="PLF20_00845"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009436729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOX79589.1"
                     /translation="MTIKIVNNSHHPLPVYQTMGASGMDLCAYLHESVEILPLHRNII
                     PTGLFVEIPEGYEGQIRPRSGLAIKHGVTVLNSPGTIDSDYRGEIQIILVNLSDIPFI
                     VNDGDRIAQFILMKIEKIIWQIELELGATTRAARGFGHTGLSSNHK"
     gene            37133..38866
                     /locus_tag="PLF20_00850"
     CDS             37133..38866
                     /locus_tag="PLF20_00850"
                     /inference="COORDINATES: protein motif:HMM:NF020855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX79590.1"
                     /translation="MKIFNINLFLIYFALLCASNAFAQIQDTLPNKFIGENIRIDEQA
                     KYLEALKFVLQGDDSTAAQILALILMNNPDNHTAAFELSKIYYSQGNFYASAVLMENA
                     VKLNPNNYWYWKQLLDSYEATGNEETYMKYFALFISRFPDKPEDYRKYLDVLIKNQKY
                     DEAISFIDKLISKNIVNEEIIIKKFELLLKKDKKKDAICVLNNYLTKYPCSESIMLVL
                     SNYYFQNKKDKDALSILDDLLNCDTNNEIASLTLAEYYNKKGKNDKAFKYLLKAFNNP
                     QITVNNKIQYLLNFYSLDNIKASDTAQILKLVGIISQVHNQEPNSKILAGNIYFVSQY
                     YAEAAKEYEAAIDLKVNNYEVYEKLLLCYAALENNDKLKSLADKVIELYPYQPLPYYF
                     SGFVSFIQKKYTESGELLEKSLKYSGRNSNLTQFLYRLLAEVNSNLQDYDKSDYYFEL
                     ALQSDSSDATLLNNYSYSLAERKKDLEKALKMSEKALSINPKEPSYLDTYGWILYRLG
                     RYDEAIVYLKKAVEKTDNTNDAVLWEHLGDAYYKIGNIDEALKNWMIASEKEGSSEKL
                     LQKIKDKMLYE"
     gene            38859..39668
                     /locus_tag="PLF20_00855"
     CDS             38859..39668
                     /locus_tag="PLF20_00855"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HOX79591.1"
                     /translation="MNKNSFILLILFSWLLIITSCKVKEKPISTPYPQTFRYPISDYF
                     KMHTPHKFLTCKANVNYYKDKQDYSFYVNSRIIYDSAIWLSVSPGLGIEALRIMLTND
                     SIFYLDRTRHKYITESLQIIRTKWNLPFDIKVFQAIITADFLRQSDSIIYTYKKTDKG
                     LRVINNNANYIDSMFPLNNPKIIDVYLNEKKPVTTSVNFDINSCKISTITINYKEHKL
                     IDNEYLPTNWNIYLQGKESKKIDISIDKWQKTDKVVLHFVIPPDYESLIIY"
     gene            39685..40926
                     /locus_tag="PLF20_00860"
     CDS             39685..40926
                     /locus_tag="PLF20_00860"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOX79592.1"
                     /translation="MNDLLKLLIIILILVPGKSIAQKSRTDLENQRKLLEQEINETTQ
                     KIKKAQTTQKNITTEITLINKNINDRKRLIESLTKEIQIVDLNLEQTTKEIKRYEKML
                     EANKKEYEKLLLNFQIYTNQPNDLLQFLFASKSFNQALLRLKYYRQYKNFLQERMLML
                     KEIQTRLEVQQKELSQQKQTKYQLQQLELEQKNNLEVTQKKKNRLLQDAKKDEAKLKS
                     LLAEKKKAAETLNAAIRKLIEEEAKKAAATSNKSGNKEYKSSDGFVTFYSAEDLKLSV
                     EFEKNQGKLPWPIERGLISEYFGEHPHPVLKGIKVKNNGLNFICPPETPVYSIAKGKV
                     SKIMEIPGMRNVVIIRHGEFLTVYSNLKNIMVKEGEMIAAHTKIGTASEIKNEENAEL
                     HFELWKGKTPQNPLPWLSSKN"
     gene            complement(40928..42013)
                     /gene="lpxK"
                     /locus_tag="PLF20_00865"
     CDS             complement(40928..42013)
                     /gene="lpxK"
                     /locus_tag="PLF20_00865"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOX79593.1"
                     /translation="MNILKLLLLPFAFIYGFITSFRNFFYNVGIFKSYAFDIPIISIG
                     NLCMGGTGKTPHCEFLIQNLYKQYNLVYLSRGYRRKSRGLQIIKIDDNSQKAGDEPLQ
                     IKRKFPHIVVAVSRNRVKGIQEILNNFPQTQIIILDDAFQHRSVRPAINILLSNFFHL
                     YTKDNLFPIGSLRESRRGYKRADLIIVTKTAKIFPMMLKNIVEHEIKPLPYQKLFFTY
                     YKYSNFLPAISNNIYKKNPKTSRILLFTGIADANPLVDHLKLNNYRVEHISFPDHHIY
                     SKNDIIRIIKTFENMIGTNKIILTTEKDLMRIRGNQEIEGILNQYPLYYILINVKFHF
                     DEEQKFLSLIAKMIDTNLPLRQKDIEF"
     gene            42143..43246
                     /locus_tag="PLF20_00870"
     CDS             42143..43246
                     /locus_tag="PLF20_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOX79594.1"
                     /translation="MITVRDVLQVLDQYAPFVYQEEYDNSGFQLGDKDEEVHGILLCL
                     DLTTEIIDEAIQNRFNLILTHHPFFFKGLRYLDFSKSEGRIIRKLTENRISLISFHTN
                     FDNVAKGTNEALANKLGLKDTMILCPKKGALRKLVTFCPVDFSEKVRSALFSVGGGYI
                     GNYDSCSFNAEGYGTFRAQEGSHPYVGEINQIHKELETRIEVIFPVHLTDTIIRLLLE
                     VHPYEEVAYDIIPLDNVYTSVGEGIIGNLPQNFELMDFLAFLKSSLECKSIRYNTLSN
                     NKIISKVALCGGSGSFLIEEAVKLNADAFVTSDLKYHNFLDSAIENMLLIDAGHYETE
                     IGGLNLLKEFLLQKFPNFAVEISQKGLNPVNYL"
     gene            43291..44079
                     /locus_tag="PLF20_00875"
     CDS             43291..44079
                     /locus_tag="PLF20_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOX79595.1"
                     /translation="MDTNANLQNPQTEENVELSIEQKLTNLYRLQQIESQIDRIRIIR
                     GELPLEVQDLEDEIAGLETRIDNYIQQIAEYEQKILEKKNIIKDCQALIKKYEDQQMN
                     VRNNREYDALSKEIEYQQLEIQLANKRINEFTNELNHLKEEIEKAQKLVEENKSELEI
                     KRSELADIIAETEKEEKELLQKAEKCRKLIEDRLLLAYTRIRKNARNGLAVVHVERDA
                     CGGCFNKIPPQHQLDIRMHKKIIVCEYCGRILVDQAIVDSVNQS"
     gene            44474..45700
                     /locus_tag="PLF20_00880"
     CDS             44474..45700
                     /locus_tag="PLF20_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79596.1"
                     /translation="MESGDQNSPYYRYCLGHFHFQWAIIHLKFKDYYTAFKEANTAYR
                     LLEKNYRLYPQFPPSLSALGVLHVFVGSVPDQYNWIKNLLGYKGTVEQGLSELETALE
                     ESMQNNDLFWLEKETVFYLSFIYINLDKGKEKSLSFRNKYLSQKDIELEKLDPIQAFA
                     FSKVSLFTGRNDEVIRILRSRKFYPDQTEFTFLDYLLANAYQNKLSDSCLIYYDKYLS
                     YPTVKNFKKSSLQRKAWQYLIKEDREEYYLLMKQVLKTGQEEVDADKQAHFAATKGII
                     PNVLLLKSRLLFDGGYYNQALNLLLENKDKLNTLPIIEQTEYHYRLGRIYDETHNLVM
                     AKLEYHKAIELGKALPQYYAANASLMMAMIYEKQHQYPLARHHYKMCMDMPFEEYRNS
                     ITQKAKIGMERTKKMK"
     gene            45728..46213
                     /locus_tag="PLF20_00885"
     CDS             45728..46213
                     /locus_tag="PLF20_00885"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX79597.1"
                     /translation="MISNNYERMLKLVDEVFATSSDPNQIRVTPEVMDRLKEMHPSTF
                     AEYSDSNGPVAWIMIIPSTLELMNCFVKGEISENELFELTPSNISYEALYLCSALVLE
                     EYRRQGIAKRLILQAVENIRQQFPIKALFVWPFTKEGELIAENIARKVSLPLYKRLEN
                     K"
BASE COUNT        15181 a         8101 c         7749 g        15435 t
ORIGIN      
        1 tatttaagca tagacagatg gagatcaaag gtttctctcg aaatagtctt ctattttttt
       61 gataagatgt gctctgtctt ttccagcaac attatgatcg tggccggggt aaataaaata
      121 atcgagcaaa attccttttt ctatgcactt ctgaacgaaa tcgatgctgt tttgccagac
      181 aaccacagga tcagtcgtac aatgtattac aagcagtctt ctatttaaac aattggcttt
      241 cgaaaggaga ctcgaatttt ggtaaccttc cggattttct tcgggagtat ccatgtaacg
      301 ctctccatac atggcttcat aatatttcca atcgcatacc ggtccgccag cacatcctgc
      361 cttgaaggtt tgggggtgtt gcaacattag attgatcacc ataaatccac cgtaactcca
      421 gccatccaag ccaattctcg tagtatctac ataatgcagt tgtttcaaat aattgatgcc
      481 gcatatttga tcttccactt catacattcc aacatgtcta tgtataacgc tttcgaactc
      541 aaatcctcgg ttggcactac cccggttgtc caagctgaaa acaatataac ccctttggct
      601 gagatagttt aaataaatgc ccgctccgct caaccacgaa ttcctaatta actgattatg
      661 cgggccacca taaacataaa caattacagg atatattttc gaagtgtcaa aatcgggagg
      721 tgtaatcatt cggcaatata aatcatcatt atcgtcattc ttaatcgtaa atacagtggt
      781 ttcccctagt ttataatctt tcaaaggatt ctcggccgta aatatgatct tgtcaatttt
      841 agcattcagc ttattgagtc ttatcttcaa aggaacttcg ctattagaat aaatatctaa
      901 aaaacaatcg ccatagctgt tgaattttac cgagtggctc ccaccttctt catttatgcg
      961 tcttacctca ttattacgta tatctactgt aaaaatattg ctttcgagtg ggctgtcctt
     1021 tgtggaaagg ataataaatt tgtgtaattt ttgtgaatat cctgatattt cctgcacctc
     1081 ccaattacct tttgtaagct gtttgataag atttcccgat gtatcgtata aataaagatg
     1141 gttatatcca tctctttcac tctgccaaac aaactgtgca ggatttgccg gcatgaacca
     1201 tggaccattt tcaggttcta cgtaacgcgg agaggtttcc tcgaaaagta tccttttcag
     1261 atttccagta gagacatcat agcaattgag ccacaaatga ttttgttccc ggttcagaac
     1321 agctatataa atgctcttat tatcgggcga ccatgtaata ttggttaaat aatgagccga
     1381 tggttcgggt gtgtttagat aaaccgtttt ttgggtcagt atgttgtaaa ctcccacagt
     1441 aacatgatga ataggcatgc ccgccatagg atatttaata ttattgactt ttgcaggcct
     1501 cgacgtaata tctattatgg gataatcggt aaccatagtt tcatccatcc ggtaaaatgc
     1561 cagtttggtt ccatcagggc tccagaaagt tcccttactg attccccatt cattgcgata
     1621 aactctttca ctgccatata aaattcccga attcttttca tcagagatga ttctctgttc
     1681 tccccttaaa ttaatataaa gattattatc aagggtatag gctatatgct gagaagattc
     1741 cgatatatcg acattgcctg cgttttcggg tatttgagtt aaaagaacaa ttctttggga
     1801 ttcaggatta cataaataga acttattccc tttttgaaat cggaattcat ttttattgat
     1861 ccaggtcatg cgaggaaatc ccttcccggc tttctcgttg ttttgcacca gaagctggtt
     1921 gaattgctgc aaaaaaatga gagtgtcgga agtttctttg gtggcttctt ttttaatcag
     1981 ggctttttca ttcgtgaaag cgtaaaaatc agtttcggga atccatgata gattgctcaa
     2041 tcctttgggg tatagtgccg gattattgta tgaagcatct tcagcagtaa agtgtttttc
     2101 ctgagcttgc gaaatgagca cataattcag caataaaaac agtaccagta atttcttttt
     2161 cgacaaagat aaagatgtat agttcattat agtttataga ttatttttta gttgggacat
     2221 tttgttgttc ctgttcatca taaaccttag cagtattgag aaggctttcg atctgaagca
     2281 tgtattttct ctgttccgaa ttgggatgat atagataacc ttctacagta acgattcgtt
     2341 gacgttttgt atcggcaaaa gtatagctga taaagggtcc acccataaaa tcgtttacta
     2401 ctttccagag tccacgtgtt tcgacggcaa aacccgtcga aaatttagag gttcgcttaa
     2461 attgtggagg aataacgtct accgaagtgg ccatatatga accttcgact gctccaggaa
     2521 tatattttcg ggtaatagaa tctcgtgtac gaataatgtt tttaccatta aactgagccg
     2581 tatcggtata tggcctgtaa tataccagaa atcccgtttc tgtgtcttgc ttgcgacggt
     2641 gaatagtttg tcttatccaa ataaaatttt cttcttctct ggcaactgtg aatccgccgg
     2701 gaataagcat atctattcca tatttggatt gaagcttagt aataatctct ttggccggat
     2761 ttgaggtaaa aagtttctga atacgatctt tttctacctc cctgtaaagt tcatataaag
     2821 aagggtagta ttgattaaac aagcaaaaaa aggaagtatc ggatggacaa gaaaagtgaa
     2881 tgatcaattg gggttcagcc caataattcc tttccagatt tatggatggt tttgttaatt
     2941 ttgggtctat acttacgata aagatattgt gatgagcctt atacatatca ttttcgtaaa
     3001 gagtcttgga aagtattggt aaaggagtaa acgattcctc aggcatagga agacagggaa
     3061 tatatcgagc gaacagagaa cgtatggtat ccccgatttt accattccat tgctcatgac
     3121 tatcggtgac aaccaaaact tcgagtgtac caccggtcga agattttttt gaactgcttt
     3181 tggaacagga tgtagcagtt atcaatgata gaaaaactat aataaccgtt aataattttt
     3241 tcatggcttt attaatttat tactttgatt ttcaattttt gaccaaccga gatcctgttc
     3301 gaacgcaaat tgttcaactt tttgatttca tctaccgtaa ctccatcgaa ctgttgggca
     3361 atatcccaaa gtgtatcccc ttccttcact gtgtaatatc tatactttac ctgtttgttt
     3421 atctcagaag aagctgaatc attctttgcc gcaggaggat gaatcaatag tctttgattc
     3481 acctgaatat tatatccctt taaattattc cagcttatca gctcattgat ggtacaatta
     3541 tattttatac taatctgaga aagggtttct ccccttttaa caatgtggta gttcggatgt
     3601 tgttctttat tcgtcgaaga attcgatatt tcaggcttgg tatcggttgt ttgattgtta
     3661 ttcgaggaac tagtagaggc tatcgatgag gtaccggaat aaataatgag tcgtttaccg
     3721 gctttcacgg tatttgattt caagttattc cattttttaa tatcacttac cgaacaatgg
     3781 tatttgcgag caatagacga taaactttct cctgaccgca caatatgaat ctgctgttcg
     3841 tgtatatttt taatttgttt gaggagtttt tccttttcta ttccttttgt agtagtataa
     3901 ttgtagatag tgtattcatt gtttataaaa tctccgataa actcacgggg taactgcaag
     3961 taatatttct gatcttttgc tacgggaatg atacctaact tgtaggatgg attcagaaat
     4021 ttgatatcct cgatgggaac gcccaatact tcactaatct gatcgaatga gaccggttcg
     4081 cgaacaacga ctgtatcaat accgttgtat aagattccgg gatgaagagg gtagagattg
     4141 tgttcgggag catactgcat aatataagag gctgcaatga aagccggaac ataccctcgg
     4201 gtttcggctg gaagatatgg ccagatggcc cagtaatttt ttgtccctcc tgcccttctg
     4261 attgccttgt taacattacc aactccagaa ttataggctg ccagaaccaa aaaccagtct
     4321 tcatagagat cgtacaaatc ttttaaatgt cggcaagctg catcagtcga taaatatgga
     4381 tcaaaacgat cgtcaaccaa tgaagtagct ttcagaccat atactttccc cgttccatac
     4441 ataaattgcc ataatccttt tgcccccgct cgtgaaccgg ctataggatt aagagctgac
     4501 tctatcaccg ctaagtattt cagttcgaga ggcagctcat acttgtcgag cattccctca
     4561 aataacggaa aataaatata cgacagcccc aataaacgct gcgttaactg tcgtttttta
     4621 accgcataca gttcgatata atcctttaca taacagttaa aggtataatc tatgggtgaa
     4681 ttctgcttca tgttctcaat tctctgctga taaaccgaat ccgaatatac tggtataaaa
     4741 tctccgggaa actgatatac attggttaaa ctcgcatcag cagtaaaata ataatcctga
     4801 aaaaatttaa tattggctaa gctatcgagc gcacgtaaaa tcggtgaatc ctctataagg
     4861 tcaacttcgg gattaaagac cttcttgtaa ggtaagaaaa aaattcctga atccgaagta
     4921 tgtatagtat catgaacgag tactttctga gattgtcctt ctaatgtact aagtaaaata
     4981 aatagaatga gtattttcct gattcctttc atttctttta ttgagttacg taaagccctt
     5041 ttcaagatat gaatgacgat atctttgcaa aaataatcta taagctcaaa attttatcaa
     5101 tttttgataa gtttgtcaaa atcgggatcg aattcttttc gatttaatag accctcatcg
     5161 tattgagccg tttaattttt ctggattcat tccatgcctt ttatttttaa tctattgtaa
     5221 tataatactt tataatcttc taccacatgc tttgctatca gaatcctgaa aattaatcac
     5281 ttacaatctc ttaaccccac cccattcatc attccttaca atgaatctta cttgcttaac
     5341 aatgtcaatc gagcattttc agatcttaca aagcatttat gatttgcctg gcaacaatac
     5401 ccatgataat tccgctcgac tcaggagtgt atctctttat ttcctgtgta tgggttgctt
     5461 ttcactttct tagcggtcat atcgtcccat ttatgctaat gattttcaat cttataaaga
     5521 acaatctgcc acttatccat tgggatttcc gaaagaaatt gctggataac ctcatttttt
     5581 atctatcttt acctgctttt ttcttttgtt tttctttaaa gatgatccct ggtatctctt
     5641 tcaattaaca tcattcgtaa tctataaaat aggtttttat gcgctctaag agttttattc
     5701 ttctaaacgt tttaatagta gtcgctgcct tgtgtcattt cacctttgca caggagcctt
     5761 tacaatgtac ttccatagtt ttaacgcatt ggatgacacc cgatgaattc ttgcgtcgaa
     5821 gtgagattgg tcgaggattt actcctaccc ctcccccacc tgctccagtt actaatctgg
     5881 ctgagtttaa ctacaatgaa ggcgttttaa tacgctatcc ttttggaata cctatggcat
     5941 taataaaaaa aatgtctgaa gttggtattg ttattaccat tgttgctaat cattcagcgc
     6001 aaaatacggt ttataaccag tatgttaata atggggttaa cttgactaat tgcagttttt
     6061 tgattgctcc aactgattct tattggacga gagattatgg gccctggttt gtaatcaacg
     6121 gagaaaacaa agtgggtatt gttgattttc cttacaatcg tccacgacca tcggatgatg
     6181 atataccggt caaaatggca agttttatgg atgttcccct ctatggaatg aatatcattc
     6241 atacgggtgg aaattacatg tgcgacggaa tggaagctgc tgcctctacc aatctggtat
     6301 attcagagaa tccttcccta agtccttcgc agattcatca aatgatgaac gattatctgg
     6361 gaataatcaa ttatcacgtt cggcccgatc ctaatgggac ttacatcgac catatcgatt
     6421 gctgggcaaa atttctcgac gtcgataaaa ttcttgtccg tcaggttcca tcttctcatc
     6481 cacaatataa tctgatagag caagctgcag cctattggca atctgctata agcagttacg
     6541 gcactccata tcaggtattt cgtgtaaaca caccgaacaa tcaaccatat accaactcgt
     6601 ttatcctcaa ggatcatgtc ttcgttccct tcaccgggaa taccctgtat gataatgagg
     6661 ccttgcaagc ataccaaaac gcaatgcccg gttatcagat cattggggtt tatcaaaatc
     6721 cttctactcc atgggaatct accgatgctt tacattgccg cactcacgaa gttgccgata
     6781 aatatatgct ttttataaaa catcttccta tcgtgcatac tcagccttgc gacgaagatt
     6841 atcttatcac gacatatatt aaatctttgt gtgattccgc catcattgcc gattctaccc
     6901 gggtattcta tagcatagat aatggtcctt atcagatcat acctctcagc tatcaatcca
     6961 atgatcaata tacagcactt attccacggc agcctgccgg aagcagtatt cgttacttct
     7021 tgcaagccgc tgagaaaagt ggccgtaata tgtatcaccc cttcattgga gtcaccgatc
     7081 cccataattt tatggtaggc gaaggaattc atccacatat tacactttct acgcatagtg
     7141 tagatacttc ctccaatccg ggtcaggtag taaatttccc tatttccata accaatgttg
     7201 gttatgctga ccttgcctgg attttacaaa tcgattcggc ttcatcgtcc tggctctatt
     7261 cttccgatat catggggaca ttatctcctg gatcaactca agatattacc ctgactctcg
     7321 atgcaacttt cctcagtccc gcgctttatg aaagcaagat atccatccaa tcgaacgacc
     7381 ccgatcagcc tgtagataac attctcgttc attttaatgt cttttccggt attggaatcc
     7441 acaacgaaat agattggctt gcagattgca aaatagggcc taatcctttc tccaatcaat
     7501 tgctaatcga atttcgtcta aatcagtcta tcccagttat aatagaaata tataattcat
     7561 cgggctatat agttaaaact ataagtaaag gctatcttcc ttcgggggta aatcatttta
     7621 tctggaatac ccccgaatct tcatttatat cactacccga tgggctttat cttgtaaaaa
     7681 tttctaccga caaatcacat aaactcataa aagtgatcca taagaattga tttaacccag
     7741 cttgagcatt ttaccctcaa aaagttgaat taattatttg tatttacatg aggctatttt
     7801 acaggaaaaa tttgtgttcc ctgaaagttg aaactcaaaa attcaacttg ctgtatacaa
     7861 ggaaattgca tccctaaggc tctcaagttg ggttgggtta aagcgggcac atatttgtta
     7921 caaattggtt tccacgataa aacttccatc caaataaagc cgatgattat ttgagtatga
     7981 ttctcccaag tttcttcgag atctgagatt tcgaaacatt taggtcattg agctgcttta
     8041 gcaaattcca ggcttcttcc caatctgctc gttgtaattc ttgctgtacc ttgtttattt
     8101 cgagctctat gcgtcggagc cggagattat aaactgagtt gatgactaaa tcttttaact
     8161 tgtctatttc tttaggtgtg aaaattccct tttgcttaaa ccaattttca ctgatctgat
     8221 aattggatgc taaaaaattc atagctatat tagctatttc ctcatcagga tgattgataa
     8281 aatatttttc aggcagtatg atattttttt cgaacgctgc tttgtattcc tggtaaagtt
     8341 tttgaagaac tggtgagcta aattcataat catcctcgat tagttcactt acaatgaagt
     8401 gagatgtatt cactttcata aattgttgct ggccactttc atcatcgaac tggaactcga
     8461 aattcttttc tccatgattt aggagcaaac gaatgatgtt gtattcttga tgagtaatgt
     8521 ttctttcttc agcaatttcc tgatttgaat ctatctgaag gtttggcggg gaaagaggtt
     8581 gaggctcggg tttaaagttc ttgtattttc tcgaaagtat cttgttcagt tcatagagca
     8641 gagtttgctc aggtatattt aaggtttccg aagtatattt gataaattct gatcttgcaa
     8701 tgctactggg cactaaggcg atggaatcga gaatttcttt aatcaaagcc gatcgtttta
     8761 tgggatcatt ttgtgtttca tcgagcagaa cattggtttt aaaacctata aagtgttgag
     8821 ctttttcggt aataaaactt actacttctt cggtacgatg gctgcgggca aaagaatcgg
     8881 ggtcatggtc tttggggaaa aggacaattc ttacattcat cccttcctgt aaaatcatat
     8941 caatgccgcg aaatgatgct tttataccgg ctgtatctcc atcatagagt atagtaacat
     9001 ttggagtata tctcttgatc aaatgaattt gtccaggagt gagtgatgtt cctgatgatg
     9061 ccacaacatt gcttactcct atttgattca ttgaaattac gtcggtatag ccttctacca
     9121 gataacagtt atttgcatcg gcaatttgct ttcgtgcctg ataaaatcca taaagaacgt
     9181 tgcttttatg atagatttcc gtttcgggag tattgatgta tttgggaaat aatttgtcat
     9241 tggtgaggat tcggcctcca aaaccaatta ttctacccga ttgattgtga atggggaaga
     9301 ttacacggtt aaagaatcgg tcaatgtact cattcccatg ttttataata agccccgctt
     9361 tttctatgta atcaggatca aatcctttcg aaagggcata tatataaagt gcatcctttt
     9421 gcatgggaga atatcccagt tgaaatctct gaataattgg atgctgaaag ccacgttctt
     9481 cgaaatagga taatcctatg tttcttcctt caggtgtttc ccataattga gttttaaaga
     9541 attcagcggc catggcattc accgaataaa ggctttcttt ctcgtttgcc ttttgaattt
     9601 cttcttccgt tggttcacgt tcttcaattt cgatattgta ctttcgcgcc aaatatctaa
     9661 gtgcttctat ataagtataa tgctcatatt ccataaggaa tttcacaaca tttcctgcag
     9721 caccacatcc aaaacactta taaaagccct tcaagggtga tacggtaaaa gaaggtgtct
     9781 tttcgctatg aaatgggcac aatccaacca gattggcccc tcttcgacgt aaatgaacat
     9841 attcgccaat aacttcttct attcgggcgg tttcaagaat cagacttact gtggactcgg
     9901 gtatcaagat cgaaaaaaat ttttcaaaat tattacgaat ggagcaaata gattaggtaa
     9961 agttaaaaaa accagcttgt gtaaagctgg tttctagaaa aaagaatttt ttccgcctct
    10021 tgtggaaatt ccatcaatag aacaacacac gattatcctc aatttctgcc agtttttcca
    10081 gcgctttgta agttcctgag ctgctgcggg caaaaaaatt gttttcgagt tgtttcgttt
    10141 caaatgtgaa atctgacttt gccggagcat cggtaaagga cttcacctta gcaacaaaag
    10201 cattgttaat accttcgata ggatcggaga cttcattggg attcagcgaa aaaactgttc
    10261 caataagatt tggctctctt cctatgccag gaatattata ggtgtaaaaa gttatatcag
    10321 aagctgtatc gaccttggcg tttaatctaa ttgccaattc atacaggtcc tttgtttgaa
    10381 cttctttcat ttttgcagcc agcatttgaa ctttcttttc ctttttcacg gatgcttcaa
    10441 tgccttcttt gatatcttca agtggagcaa tgcctttggg atgaattttt tttagtgtgg
    10501 caactacgaa tttattgcca aattctttca catcggaaat cgctcctagt tttacttctt
    10561 tgctaaatgc ccagcgaacg atatctctcg aatcatcgat accgggcaaa cccatatcca
    10621 ttgcattcaa ataatccttc gaccgtttgt tcaaccctaa ttcctgaact tttttatcga
    10681 aatcatctct tgttttacat tgggcagcaa aagtacttgc ttgagcataa atcttttgct
    10741 gagtacgagt actcggatta atgactttct tcaaaattgc tactcgtact ttcttaattg
    10801 gagtcgtttt ccctgtaact tcaattacat ggtaaccaaa cgccgattcc acagtagtaa
    10861 aatcaccaac tttcgttttc aacactgctt ggttgaatgg ataaatcatc tgaccatcgg
    10921 caaaccagcc taaatctcct gaattttgtg taacggatgg atcatcggag aattttgcag
    10981 ctatttcggc caattttttc ttatcggctt ttaacacagc catcaagcta tctgcaagtt
    11041 ttttggcttc ctctctcgtg cgctttatgt cttgactggc tccataagcg cctttgtagg
    11101 aaataagaat atgcgacgcc ttgaggctat caggcctctc ctgaacatcc attagtttgg
    11161 ctatataata aacgttattg tccatccatg gtcccgccat tgttcctatg ggtgagttaa
    11221 acataatgga atcgatctga acgggcaagg tgcccttctt tttaaatgag ctgtcgtact
    11281 tttcgtcgga attaaaattt acaaaattga cgatatcggt cgtagtggac atttcctcgt
    11341 ataaccgtct aatttcgttt tctgccattg cataatcttc agccgaaggc aaaacatcaa
    11401 aaacaacata atctatgtct ctgctttctt cctgttcata atattgttta tgcttttcgt
    11461 aataagcaag caggtctttg tcggtaattt tgacaaggct atctggaatc aatgtaaaac
    11521 tttgggctac aaagttgaaa gaggctttaa catttttcaa ttggtaatcc tttaatacca
    11581 atggccgcgg catgtagtag gctttaccta ttagaacatt gaattttgtg ttgagtctat
    11641 cttctcttat agcttcaatg agattttcca tttgtttctg aatttcaggt tctcgctgat
    11701 cgagggtttg aagaaagttt atcaacaatt ctctatccag aagacctgtt tgggggtttt
    11761 ggaaattctg tagaatatat ttatgagggt ttgggccttg aatcagatca gtcaactcat
    11821 cctgcgaaat gctaaggcca gtttccttga aggcttttcc catgataatc tggttgacaa
    11881 gctgattcca ggtattttgt cttacagaat aaatttcttc tgtagaaagg gcttcctttt
    11941 gggtgctttg cttggtgtat cccaaatttt cctcgacctt cttttcgaaa tcaatgtatg
    12001 agatcttttc acccgcaacc actcctatgg cggtagggtt tctccatgtg cttttaccga
    12061 tatcgcccaa aacgaaggcc aaaatagcaa cacccacaaa gattattgca acacctgaac
    12121 gttttctaat gtttcctaaa atagccattt gatgtatttt ttaaagccag caaaagtaca
    12181 gaaatcaaag caaaaggaaa aatttttctt ttcattcctg ggttaatctc ttgggtcatt
    12241 atttacaacc attatcaatc gaacccttat ttaaaaatgg tcgaatttta attttttagc
    12301 gtcattctca ccttttcgat tcttttgcca gtcgattgaa ggatttggaa acaatagggc
    12361 tcgatggtta tttgttcgtt gtttgaaggt atcccttcat aataatgtat aattaatcca
    12421 gcaatggttt catactcatc cgattcgggt aatccaagat tgtatttgtt gtttatatag
    12481 tcgatctcgg cccgtcctga aaatatatat tccttttcat tgatttttct gtcattcact
    12541 gaattagaat catactcgtc gttaatttct ccaaaaatct cttctataag gtcttccagg
    12601 gttaccatcc ccgatgttcc tccaaactca tcgagaatga tggctacact gattttatca
    12661 tgagtaagct tttttagagc tttgtcggca gtcatggttt caggaaaata ttctactttt
    12721 cgtataatgc tttgcaaatc agttggctta ttgaatagat cgaacgagtg tacatagcca
    12781 ataatatcat ctatggtcga attatatacc aatatcttgg aatgaccagt ttcgacaaat
    12841 ttattgatca atatagggat gctttcattc ttttccacag caaccagttc ggtgcgcgga
    12901 accatgcact cccgcaattt tgtttccttg aagtcgagag cggcttgtaa tatatgtgct
    12961 tcttgttcaa tatgcttatt tccagtggat tgcgtaaatt ctcttataaa tttgtcaaga
    13021 tcgacccatg tgatggaggt atcttgagaa ggcaattcct gcttcaaaat ctttacaata
    13081 aagatatggg acattttgtt aaccaaataa acaataggga atagtattaa ataaatgata
    13141 tatagtggaa tagctaaaaa attcaagata acattgggtt gtattttgaa aagtgatttt
    13201 ggtaaaaatt ctgcgaaaac gagtacgatc aaggttgaga tcaaggtttg aagaaaaaga
    13261 atcaagctgg tagacgggat gtttcctaat ccgattcttg ggaaaatctc atgagaaaga
    13321 atgggttcca tagctaggct gtataccaca agagttatcg tatttcccaa tagcatagtg
    13381 gcaatgaaag ctgagggatt ttctacaaat tttgagagta tgacagccga ccacgattta
    13441 ttgtttttag ccagagcgat tttgagccgg ttggcagtga aaaatgccat ttccgatcct
    13501 gaaaataagg ctgaaaacaa tagtgagatg acgactatga tgtagttatt tcccattatc
    13561 aattataaat cacgcaaaaa tttattgttc atcttctacc agaatttcac cggagacatt
    13621 tcttatgatt cttctcctaa aattatcatc cgattcaaga cctcgtccaa aaataatctt
    13681 atcgggtgtg gtgattttga tattggcttc agtgaatatt ttaccctttc tctggtccca
    13741 tgcaagttct tcggtttcta gtttttctcc cttttccata ttcgttacca caacattgtt
    13801 cttcacctca atgcgtttct cattcatata attgattcca aagcgtgaag tgagtgaagc
    13861 cgttatcctc atttgcttat cgtaaaaata aacttcgagg ccttgtggga aaatagtttt
    13921 tgcctcgggt gtggaataag tgtaaagcaa gggacttttc aacagacttg taactctacc
    13981 tgaatcaacg tatctaagct gaatattttt aattacttcg tcgggtaagg tatctattgc
    14041 cgtcaattcc tgaattcttt tcatatcgtt cttacaggaa acggccatta ccattaaaat
    14101 ggcaatggcc gtaataaaaa atactgaaac tctatattca taaaataccg atgagttcat
    14161 ttttacttta cctggcaggc ctaaccgtag tagtttcatt tatccaacat tccactctat
    14221 aactttcacc ctgcttcaag ccatagaaaa atatggtctc gatgcttgga aaataactcg
    14281 aataagtcgc tattcgcttt gttgcaattt cttcgactcg cgggtctaaa cggcgggctt
    14341 gcatgtattt atcaacagca gcccaataag ctgcacgttt agtgagttca ttatcaccac
    14401 attgtgaagc cgaaagtgca taagcatctc ctatgataat atatgcttct ccgttgttgg
    14461 gattgatttc cagaattttt tgtgccatgc ttctggctgc cggaaagctc ttttgagcaa
    14521 ggtaaacctg acccaataaa tggtaaattc tcgccttttt attcaattcg tctggtttat
    14581 ataactgaag actctcctcg agatattttt ttgctacgtc ccatttttct tctttgatat
    14641 attttaatgc cattaaatat gcactattgg ccgagggttc gattttatga agattttccg
    14701 ttgcagcaaa aaataattcg ctgtcagtgc attgcttttt atcgaggacc ttagtgatct
    14761 tcttcaaaag agttgtgtca ttcggatttt gattaaactt ggaatgatag atgttcacaa
    14821 ggtcttcgca cgtggcaaag ggttcgaata gtacctctat attgtttttg ataagatagt
    14881 atttctttat agcgacagaa tccgtgtgag cattatccat ggcctgatcc attaagtcgc
    14941 tcaactggtc atatacatcg actacgaggg ttttgtcagc cagttctttc tttaccattt
    15001 cgatggctgc ttccatataa acttgaaatc cactgggcag aacttctgcc ccatctattt
    15061 cgatggctct tttgataatt tggaacgctt cttctggttt atcgggttta taggtaaaac
    15121 aatccagagc ctttctggta agaatacttc ctacctgaga ttgcccattg gagggattaa
    15181 tcgggaaata tttaatcctt gcatcataaa tttcaagaag actgtcgatg atctgattct
    15241 tccgtagagg atctttctca ttttcaagac gatattccat aatcttaata ccatctacaa
    15301 aagtaaactg acttgcgcag gggcaatttt gataaaccca ccgccagtgc ctgtaagcat
    15361 ctttatagtt tttttgtttg accggttctc gataaaggga gagatttttc acacattcaa
    15421 cactgtcctg tccgtatttt gccttttcat tacaagccga aacttggcca aatactcgaa
    15481 tgcttcccaa agaaagaagt aaactaacaa tgaaataaag gatggtttta tttgtcttca
    15541 ttttataata atgttttatc taattatatt tacgttttac aaaccagcgt tctgttatgg
    15601 agatgcctat atatatttta aaattatttt ctttgatcaa ttgattatgt attgtacctc
    15661 tgttaccaaa ttctgcgcca aaattaatcg aggctgcctg ccgtctcaaa gggagtccaa
    15721 agccaatact ggcagtaaat tcattaatat tggttccgtt gagtctcaaa tacgtatcgt
    15781 tataattaat cccaaaacga tacctgattt tttgccagta tttcgatatt ggtctattgt
    15841 cggggatcca ttccacacct gcattaattc ggtaactatt tttcaatgaa tcggacttcc
    15901 cagatatagt gtaatcttcc cagaacgaat acataaaatc agcgccaaaa catagctttt
    15961 cgggaatttc gtataaaaat ccacctccaa aatgaagagg caattttaaa tcgccctcta
    16021 tatccgatat atattctatt gtatccttat agagttcata gcctccatta atacccccaa
    16081 acaaagtttc cgagagtttg tcggtattgg tgttaagttt atttccataa ctgactgttg
    16141 cacccagatt gaacaaattt cctttcgata aagttctatg atattgaata ccagcagagt
    16201 aagaaatatc actaatatga atgttatctt taatcctggt gttcaatata gctaccgaat
    16261 cagggaaatg taataagcgg ctcctttcta aattgcccgc taaatagatt gcattgaagc
    16321 caatagaaaa atttttgtgt aacttaaatg cagctcctat atagtactga ttaagacccc
    16381 catctccttg gtaataataa ttgacctctc ctaacatggg atctgtttgt ttttggagaa
    16441 ttttataacc aacggtcgaa aatggcatgg ttccgaaact tactccaatt cttcgagaaa
    16501 caggaaaacc aaagaacaag aagtctaagc ttgcactcgt agcgctctcc gaaattccag
    16561 atgttttcaa agttacgaag gaacttttca tgccaccctc aaagacaaac gacatcgaat
    16621 cataagaagt ataagaagcg ggatttatgg gattcacatt gtaaaatcct ctttgggcgt
    16681 agccaattga acccatcgtt ctatttctcg tattacttat gcttgacaag tcacctatgc
    16741 cataatgcga atagggggaa gtaatactcg attgtgaaag gcttttcttt gagagacaag
    16801 ccatcatgat tagaggaaga acaaacaata tctttctatt aacgctcatt aaaaagcaaa
    16861 attttattaa gtccttcaag cacaatattt ggaactgcaa aaatgctatt tttaaactta
    16921 ccttcaaaat acaacatatc tcctccactt aaaaatacag acaaatcaat gttttttgtg
    16981 cgaaattcat cgataaatcc atctacctca taagtcattc cattgattac ccctgagagt
    17041 atcgatgttt cggtatcaac acctaagttc gaatcaatga aacgcggttt tatcaacggt
    17101 aattttgcgc taaaattatg taaagctttc aatctcattc ttaaacccgg cgaaattgca
    17161 cctcccagat agtttccatt ttcatcgaca aaatcataaa taatacagct tccacatgtg
    17221 ataaccaatg caggggcacc cgaagatttc gaatatgcag ccgcagccgc agctattcta
    17281 tcatttccca atgtatgtgg agttttatac gcaatcttaa tcggatattg agtattaaaa
    17341 cctacttcat agaatctata ggtttgactc aaaaatctat ataacccgat gtcgtgttct
    17401 tttacattcg ataggataac agcttcagga gagtttgaaa agtatctatt ggtgattcct
    17461 tttaaacggt cggcagttaa cttccttact ttttcaaaat gcacaagttg atcattctca
    17521 aaataaccaa cttttgtcat agaatttcct atgtctatag ctatatttaa catcagcttt
    17581 tatgcaaagt tataacaatc gtattgaaac cttgagaata ttttggttga ttacacaaaa
    17641 tatttatctt tgcagcctcg aactaaatga tggtgtcgta gctcagtcgg tagagcaacg
    17701 gactgaaaat ccgtgtgtcg ctggttcgat tcccgccgac accactcatt cggcgatgaa
    17761 atggtaaagg ctcaatgatg agcctttttt gtttcatata ttattaaaag agttgattta
    17821 aaataagttg tattactttt cgaagattct caaggagaca attattactt ataaaaatca
    17881 ttgtgaaaaa atggttacca cgttgttctt cacctcaaca aatccttttt cgattctaaa
    17941 aaatgtaacc ttattctggc tatctttcac tttgatgttt ccttttttca gaagagcaat
    18001 cataggagca tggcgattca gtatctcaaa taatccatcg attcctggta gttgtaccaa
    18061 tattatatca tcactgtgaa aaattctctt ctgcggcgaa attagttcaa ctttcataag
    18121 aatcatttac tttgctcgac gaattttttc cctttttcta ttgcttcttc gattgttccc
    18181 acaaggttaa atgcggcttc tggaaattca tcgacttcgc cgttcattat catgttgaag
    18241 cccttgatag tatcctcaat gcccaccata gtgcctttta cacccgtaaa ttgttctgct
    18301 acgtggaaag gttgcgaaag gaatctttgt actttgcgtg ctctttgaac gattaatcta
    18361 tcttcttccg acagttcatc cattcctaga atggcaatta tgtcctgcaa ttctttatat
    18421 cgttgaagga ttctttttac attttgggca gtgttataat gttcttctcc tacattttca
    18481 ggggttaaaa ttcgagaagt ggattctaag ggatctactg ccggataaat tccaagttcg
    18541 gcaatttttc ggcttaaaac tgtcgtggca tcaaggtggg caaatgttgt agcgggtgca
    18601 ggatctgtaa gatcgtcggc cggaacgtaa atagcctgaa cagaagttat agaacctctt
    18661 tttgtagagg taatcctttc ttgaagcaaa cccatttcgg ttgccagagt aggttggtat
    18721 cccacggctg aaggcatgcg gcctaaaagg gccgaaactt cgcttccggc ctgagtaaat
    18781 ctgaaaatat tatctataaa aaataaaata tctcgacccc ccgattggtc atccccatct
    18841 ctaaagtact cggccaaggt cagtcctgaa agagtaacac gagcgcgtgc tccagggggt
    18901 tcgttcattt gaccaaaaac taatgtagct tgcgatttag caagttcctc catatctacc
    18961 ttgcttaaat cccatccgcc tttctccatc tcatcgataa attcatgacc ataacggatt
    19021 acacctgatg aaatcatttc acgcaataag tcattccctt cacgtgttct ttcaccaacc
    19081 cctccaaaaa ccgataaacc tgaatagcgt ttggcaatat tatttatcag ctccatgata
    19141 attactgttt tccctacgcc agctcctcca aataatccaa ttttacctcc tttggcataa
    19201 ggttcaatta aatcgattac tttgatgcct gtgaacagaa cttcattatt ggttgtgaga
    19261 ttttcaaatt taggaggatc gcgatgaatc gcataagttt gttttacttc gatattggga
    19321 agcccatcaa tggtttgccc gattacgttg aacaatcttc ctctaatgtt ttcgcccacg
    19381 ggcatactga taggtttacc ggttttgatc acgttgagac cacgcatcaa tccatccgtc
    19441 gaatccatgg caatggctct tatagtggct tcacctattt gttgctgaca ttcaagaacc
    19501 agatcagtac catcgggacg ctttacaatc aaagactcat aaatgttggg aagctcatcg
    19561 gcttccttga atgttacatc aattaccggc ccaatgattt gagatatttt tcctattcca
    19621 cttttcatca tgaataaatt tacttggcaa aattaacatt gttaaagcat caatatttaa
    19681 tattcttctt ttattttatg gattgcctct ttctgagctg aaacttcaaa aatattcttg
    19741 taaaaaatga tcaacataaa cacccctatg gtgattgcag aatcggcaat attaaacaca
    19801 ggcctaaaaa actgaaattc ctggccccca ataaacggga accaagttgg ccattgggtt
    19861 tcaatcaatg gaaaataaag catatccaca actctaccgt gtaaaaaagg ggcgtaccca
    19921 tttttcaatg tgaatgaggc aatttgataa taagtacttt cagtgaatat aagaccatag
    19981 aaacaactat cgatgatatt gcccagtgct ccagcaaaaa tgagactaaa aactattatc
    20041 tttaaaggtt ttgattctct tttaataagt agatataagt aatacccaat agctaaaaca
    20101 gctacaattc gcaagaggct tagtaatatc tttcccgaat tgcctccaaa gctaaaaccg
    20161 aaagccattc cttcattttc gacaaaatga aggaaaaacc aattatcgaa aactggaatt
    20221 tcttctccta aaaagaagtg tgttttaatc caaattttca agacttgatc aacaaacaag
    20281 acgagggcaa cagttcctat agcaagccag atggctttat ttttcgactt cattctatgg
    20341 agtacgaaga gtaatcaaaa ttcatttatt ccgtttctgt tttagcttag catcaatcga
    20401 aagcgtagca tgaggaactg ctctcaatct ttcttttgga atgagagtgc ctgtttctct
    20461 gcatattccg taggtcttgt tttctattcg taccaaggca ttctcgagtg ctttgatgaa
    20521 cttttcagtt cttgcagcaa gtttgccatt ttcttctttg gttagcacgg cattgccttc
    20581 ctctaatact ttgaaagttg ggcttgtatc gctggtgcca ttttcttcca gatttgcata
    20641 ggcttccata agtaaagcaa gatctttacg cgctttttcc aattttacca taatgatttg
    20701 gcgaaactcc tccaattctt catcggaata acgctctttt ggagcttctt cttttttcat
    20761 tgtattccct cctattagtt aaaatttcat catgtcaatt ttctatccga ttcactttaa
    20821 taaatacctt gaattcattg gctatatcta cttctatcat ttcctgttcc aattgatcgg
    20881 taaataaaag ttctttggcc aatgtttcgg agcaaatgta gtgataattt ttttcaattg
    20941 ccttagtcag ggttggatga tggtatatct tcaattgtat cttgtctgtt acatcaaatc
    21001 cactctcctt tcttatattt tgcacccggt tgattaaatc tcttacatac ccttcgtcga
    21061 taagatcatc cgtcaaggta aggtcgagag caacggtata gggtgtttcg gttatactaa
    21121 tccagccagg aatatcttcg gttatgattt caacttcttc caaatgaatg agaactagat
    21181 ccccatcaat cttcagctga atagactgtt tctgctcaag ttctctgatt tgttcctgag
    21241 aaagattctc cagggcatta gccacacttt tcattttctt gcccaactta ggccctaact
    21301 tcttgtaatc gggttttacc ttttttacta ttgtggtgtc ttcttcgggc aatacctcca
    21361 attcttttat attggtttcg tgtagtataa tatccttaat gctcgctatt aattttgatg
    21421 tatggctatt aatagccggg attaatactt ttttaagtgg ttggcgaacc ctcaaattca
    21481 ttttcttgcg caacgaaagt atcatcgaag tgattttctg agcagttttc atccgttcct
    21541 ctaactcttt gtcgataagt ttttcgttta ttaccggaaa atcggtaaga tgaaccgaat
    21601 cgtatttctc tttttgagtt attgaattca agtctttata aatctgatcc atataaaaag
    21661 gagcaatagg ggatgccagt ttagcaatcg taattaaaca ggtataaagt gtctgataag
    21721 ccgaaatctt atcttgagta taatctcctt tccagaacct tcttctgctt aaacgcacat
    21781 accagttgct caaatcttca agtgtaaagc tggcaattaa tcttcctgct ttagtaagat
    21841 cataatcatt gaagcattca tctacttctt caatcaaaga attcaatgta gaaagtatcc
    21901 aacggtcaag ttcaggacgt tgactgattg gcatatcctc ttcggtgtat tggaaattat
    21961 cgatattggc ataaagagca aagaaagcgt aggtattaaa taatgtactg aagaattttc
    22021 tgcttacatc tataattccg ttctcatcga atttcaaatt gtcccaagga ttcgaattgg
    22081 ttatgagata ccatcgagta gcatctggcc cataattttc aattgtagta aatggattca
    22141 caacgttgcc gacacgtttc gacatcttgt ttccttcctt atcgaggact agaccattcg
    22201 aaacaacagc cttaaaagtg gcttttccaa aaatcatcac ggcaatggca tgaagcgtat
    22261 aaaaccatcc tcgtgtttga tctacacctt cggcaatgaa atcagctggg aaaaacaagt
    22321 ctaactcatc ttctctttca aatggataat gaaactgagc ataaggcatt gcacctgaat
    22381 cgaaccacac atcgattaga tcgctctctc tgtacattgg cttccccaaa ggggaaagca
    22441 aaataatttc atcgacaaaa ggtctatgaa gatcgaatga attataatcg attttacccg
    22501 tagaggcatt tttacaatct ttaaaagggt ttaccttcat aaaaccggat tcgacagatt
    22561 tttcacattc caggaaaagt tgttctactg aacctataca gatttcctct tggttatctt
    22621 ttgtacgcca gataggaagc ggagttcccc aaaatctcgt tctcgacaaa ttccaatcaa
    22681 cgaggttttc gagccagttg ccaaaccgac ccgtcccggt tgattgaggt ttccataaaa
    22741 ttttgtcgtt ttcttcgatc attttatcct tcatggctgt agttttaata aaccatgaat
    22801 cgagtgggta ataaagtatg ggtttatcgg tgcgccagca atgtggatag ctatgttcgt
    22861 atttttccga cctgaaagct ttattctctt ttttgagttt cactataatt tcaatatcta
    22921 ccgggcccct ggaatgatcg ccgtcgggtt cgtattctgg tcttacatat ctaccagcaa
    22981 attctcccat ttcagaagta aattttccct ttttgtcaac caaggttaaa tagcccaaat
    23041 tatattgttt ccctacttta aagtcatcgg ctccaaaact gggtgcaata tgtacgatcc
    23101 cggttccctc cacagtcgag acgaaatcgc ctaatacgat acgaaaggga tccccatcct
    23161 ttggtttggc ataaggcaat agctgttcat accgaatacc ttcgagctct ttgcccaaga
    23221 actctgcgac aattctaaaa ggtattttct tatcaccagg tttgtaagaa tcaaaatcca
    23281 gatccttatt cctaaaatcc agaatagttg gtagcaactg ggttgcacca attaccgctt
    23341 cggctagtcc agtgtacgga ttataggtct caactaaact ataacttata ttctcaccca
    23401 cggccaagcc tgtattagaa ggcaaagtcc atggagtagt tgtccaggca aggcaaaaaa
    23461 tttcatattg agtaagccgg aaaaggaata aggacttttc gtccttaatg attttaaatt
    23521 gtgcaataac ggagttatct ttcaccatct tataacatcc aggctgattt aattcatggg
    23581 tactcaagcc tgtcccagca gcaggtgaat acggttgaac ggtataacct ttatataata
    23641 accctttatg gtaaagctgc aataataaat accaaactga ttcgatatat ttattatcga
    23701 aagttatata gggatgttcc aaatcaaccc aatagcccat ttttatggtt aattcgtccc
    23761 ataaattttt atatttcatt acttccttcc ggcattcatt gttataatcg acaatggata
    23821 ttttattccc tatatcatcc ttggtaattc caagtttttt ttctacttct atttcaacag
    23881 gcaagccatg agtatcccaa ccggccttcc tttcaacata atagcctttt aatgtcttat
    23941 acctacatac aatatctttg attgttcgag ccattacatg atgaatacct ggaactccgt
    24001 tggccgaagg agggccctca taaaaaataa atggttctcc cgtttttgtc ttctccaatg
    24061 acttctgaaa ggtatgatct tggtcccaaa catttagaat ttcattacat atcttgctta
    24121 aatctaaaca tttatattct ctatatttct tactcataga ttttatttat taaatctttt
    24181 gaaaagctac aattgagttt ttaattatca atttcattcc aacaattgcc gaaaattttc
    24241 aggaaccata gtgatatcgg ttaaaaataa agtatcgata taactcgatt tctttgtatt
    24301 gatatttaat cccagtatgg tggaatgcat gagatatagt ttctggcaaa ttattggatc
    24361 tatttcgaat gtaggttcta aatccgttaa aaattcttcc agtttattaa aattctttcc
    24421 agtaaaatct aaaaacgctt ttttcgacaa tcgcttcggg aaaggcaaag tcgaacgatt
    24481 tttacttttg atatgttccg agaaagtatt gtcaaaaaag ttttctttca aaaagtttat
    24541 aaataatgat gtggtttctt tctggtaaat ataaggaata tgaaacaaag aaatcaaata
    24601 atgatacttc aggtttcgaa aaatttcaaa tatactttcc cataatgtta tataaaaaat
    24661 attatttccc cggtaatcgt tagcaaaata caattcatca aatagtatcg aattatttac
    24721 taatgatttt acttctgtat tcaatttaaa aacattattt ataaaaatac atttcatacg
    24781 gtgattttca ataatttcct tgcctaagtc aattctgcaa gcataaacta ttgtagaatt
    24841 ctttctatcc caaacaaagg cataacgcga ttcattgtcc aattcgtcaa tggtggcgat
    24901 attattttta aatttattag tatagtatat tttttcttta ctcttcaaga aactccttac
    24961 agaataaaga attttattag actgtgtgaa atagaactga aaatcatcta attgcagaat
    25021 taattccttt tcgattttat ttatctcact ttgaactaag gagtcatttg taaatgattt
    25081 ttcattatta taaactaagt ttgtagctcg atattcaatt ttattgctaa atgaataatc
    25141 caaactgtat atttttgttc tcaaaatgtc tccaaaatcg ttaagagata aatttatgta
    25201 ttcttttgga tgaatggcct tcccaatggt tactttaatt actttccccg attttgtaag
    25261 aacttctgca ggtaatctta aacttctcaa ttttggattg attaagccta aagaatagaa
    25321 taacagtgaa ttgtatcctg aaaagtgaat aggaattatt ggtacattca attgtcgaag
    25381 caattttttt acgttgtgcg gccatggcct gtcttcaata gaaaaatcct tgaaattaat
    25441 agctgagact gctcctgctg gaaataatcc caaaggatga cccgcattaa catgacgata
    25501 ggcttctctt aatcctttga tgcttgaagt caaatccttg cgatcttcaa atggatttac
    25561 tggtaagaat atttctcgaa gaggctcaat tttgtaaagt aagaagttgg ccatcaattt
    25621 gaaatcatcc ctgatttctg atacaatata ggcgagcatc attccatcca ttcctccaaa
    25681 tggatgattc gaaatcgtaa caaaaggacc ttcttgggga atattctcca acccgcccgg
    25741 attaacagaa tggccaactg aaagttgatc gaataaaaag cccaagccat ctctgttatg
    25801 caacccagaa gctactagat catcataaag ctgattgata gaatccatct tgttcaacct
    25861 aatgagaaag tttgcgattt tcactccaaa cttgctctta agtataggta ttgcctttat
    25921 aacttcctct gtcgaaagca atgacataac tttaattttg aatcatcatt tcagttcaag
    25981 gggctgcaaa agtaaacatt tttatttatt taccattctg cccgaagcca catagttttt
    26041 gaatatcttt gcaaaaagtt tttcgagaaa tttagcctct tcttccgata tgaaaaattc
    26101 aatccataga gatttagctg tccgtgcttc tatctataca atagttactt cccttcctat
    26161 tttattggcg tatatcttag tatgtgtgtt ggtcgagcat ttaacttatt tatcctcaac
    26221 gatacgactg attatattga ttagctggat attattgata attagcatat tgtctttaat
    26281 atgtctgtta ttcttttcaa aaaaatttct gcatatttat ataaaaaaaa taaaacataa
    26341 ttttattttt gtgaattcta aagatttttt cagttttttt aatttggttc aattgagtaa
    26401 aatgccatcc aagtttgcaa gattagcatt taatcagctg actttgaaat ttaagagaga
    26461 tataaaattt atcgaaataa aaaattttat cagattaaaa aatgtgaaat atttcactat
    26521 ttctttcgtc attcttcttg ttatatttat attcagtttt tcttcattta aagaaccata
    26581 taaaagaata gtcaattatc aaactgcatt ctcgaagccg gatttactac agtacacaat
    26641 aactcccgat aatctgatat gcaatgaaaa tgatgatttt gtcatcaatt tgaaaattac
    26701 tggtaatttt attcccgata aaattcaaat catcaaaggt atttatactg attattttat
    26761 taaaacttca gaaaaagaat tttattataa aattcatgat gttcataaag aaataagttt
    26821 tgaaatcgtt tgcaaaactc tcaatcatca taagaaatat ctactcaaag tccggcccaa
    26881 atcatttttc aaagatttag aaatattggt aatcccccca gcttatttag gactctccaa
    26941 cgatacaatc tatgatttgc gagaattatt tatacctgag ggaagtttga tacatttaaa
    27001 cctgaatttt aataaaggag attctatttt catctcgaat gaattgacaa attacaggga
    27061 aacattcaaa ctggataagg attattttac gaaatcgttt tatgtcttcg ataatactaa
    27121 tattaatttc aagttattct gtgatcgtat aatttccgat tctctctcaa tacatattaa
    27181 tgccgtcaaa gatagacctc cattgattta ttctgaatta ctatccaatg cagaatacat
    27241 tgccaatttt atcctaaata tcaatgacga ttataaaatc aataaaattg gttatgaatt
    27301 tattaaaata aattatggaa atttaaaaat aataaaaaat ttcaatcttt ttacaaattg
    27361 gaattcctcc aattttagca ataatataga agtaaattat aatgaatttt ctgaaataga
    27421 cagtcttggc attcgattct ttgccgatga ttattatcct tataaaacga atcataaagc
    27481 atattcgccg attatcattc ttagacacaa agaatcattg agttctatta tcagcgaaat
    27541 caacaattca caggaaaata ttaatcaaat cgataattca ttaaaaaatt ttgaaaatct
    27601 gcgttcaaag attaattcta tcaataatat aaatcaattg gacaattttc agaaaaagca
    27661 attaatcgat gaaatacagt cgacatatga tcattttagg aaaaacagtg atcattttat
    27721 gaatcaaaat aatttgaaca ccgatgaaaa gactgattta aaaaactttc taaatataat
    27781 tgacagtatt tataataatt tgaaaaataa tttggattta aataatatca ataatttgga
    27841 tttaaataag caaataaatg accttaaaca acttgtcgat attatgaata ataaattcaa
    27901 aaattttttg gatcgaaata tttttgagaa tatattcgat agattaaacg aattgataaa
    27961 caatcagata agaaataaca ataatttgat ttataaaacc gatagttgga aaaattctga
    28021 tacagcttat gattcgaaat taaaagaaga attaaatcaa cttataaaga agcatgaaga
    28081 tataaagaat gaatatgata aagctgtgaa agagaatttt ataaataata atctaaaatt
    28141 tcaaaatgat tccattctga aatccattag caacgaatac gagtttctta aacaaaattc
    28201 gtccttaatt gataagggac taaaaagaat caatgaaagt tacaatgaat ttcaaaacaa
    28261 tctacaaaat gaaggaatgg tcaatgaaga taaattaatc gacgaaaatg ctttaaagag
    28321 tcttttgaaa agtttaatat attttagtaa acaaattgag tattcatcaa caaattcatc
    28381 atattatcga gctttgccca atgactctgt ttttacaaat tttatccatt tttcgtataa
    28441 ttggtcatcg attaatgata gtttgaatcg gtttctgaaa caggccgata ttccggtttc
    28501 ctctatagca aatagttcat taaaaattaa taaagccata aaagatgcta ataaagccat
    28561 aaaggaaaat aattacctaa attttagtaa ggaaaccaat gttatcatgt cggaaattaa
    28621 tttaataaat ctcagtttat cggaacttct caaaagaatt gaagagaata acaagaataa
    28681 taccatggaa atggctggca tgtgcagtaa acccatgaaa aagggtggaa ataaacagca
    28741 gagtactaat tctttgagtg aaattatcaa gatacaacaa cagcttacag aaaaaatggg
    28801 tcaaaaacag atgaagggtg aaaataattc aggcttcggg aataaggaaa tcaatgaata
    28861 tattaattta caatatgaga ttaggaaaaa actaagtgaa ttattacaaa atgatgacat
    28921 gcaaaaatat tctaaaattc ttgaaagcat taaccatgat atgcgcgaaa gcgaaaggaa
    28981 acttttaaat aagaattcaa acccaacgga agctcttaga cggcaaaaaa atattgaaac
    29041 acgtttattg gaagcagaga aggctattcg cgaacaagaa tttgaaaata agcgagtttc
    29101 gaaagatgcc ggtaattata tacggaaatg ggacgaagtt gctgaaaatg agtatatttt
    29161 aaaaaaagaa aaatctttgg ataatttgaa actaaatgaa gtgcctttac gtatatttta
    29221 ccaaaatgct tttctaaatt atcaaaatag ttctaaaaat gtcaacttta cagataaatc
    29281 tcgataaacc tcagaatatt agtgtaatta taaattatat tctttctaaa tttcatatac
    29341 cttattcatt gataagcccg cttgaactat cggtcgaaga attttttcat acagttaaga
    29401 attattgtga atatgtaaat ttttctgtag aattaaaaga atcggtttta attttatatt
    29461 ttaaagttga tagaatccat tgccacacag ctaatgattt aaataataca ctaaaatcga
    29521 acattatatt aaatagttta gtggataatc tatatattga tggagattct tttgctttat
    29581 catattcatt ttcttattta aacaaaacaa tttattatga tcgtgcttcg atggtgaagg
    29641 attatcttaa tcaagtagca ttaagaatat aataggaatg atatcgtttt attctattgg
    29701 ttttaacaat cccttaaaaa aaatccgaac cttaagatgt tttctgatag gcatcttgaa
    29761 tgaacattcc aaaaaggacg gcaatattaa tattgtattt gtcgatgatg aatatctatt
    29821 gaaaatcaat aatgaatttt tacatcatga ttactatacg gatacgatta catttcccta
    29881 tcatttaaaa cattctaaag taatcgaagg agatattttt ataagttatg atagaattaa
    29941 ggagaatgct aaactataca aatgttccgt tcaaattgaa ttaattcgtg ttatcattca
    30001 cagcattctt caccttttag agtatcagga taaaaatggc aataatatta tttttaaaaa
    30061 gcaagaaaag tatcttcaag attttttaga acaattataa ttttgaaatg tttcacgtga
    30121 aacgattaat gaatagagat tatgatcttt gatcattatg atattgttgt agtcggagct
    30181 ggccatgctg gatgtgaagc atcagctgca gctgcgaatt taggttcgaa agtactcctt
    30241 attaccatga atttactaac aattggccaa atgtcgtgta atccttctat tggaggagta
    30301 gggaaggggc agattacccg tgagatagat gctttaggag gttatacagc tttggtttca
    30361 gataaaagta tggttcaatt tcgaatgctc aacaagagta aagggccggc tatgtggagc
    30421 ccgcgagcac aaaatgaccg attgatgttt cacgtggaat ggagaaaagt attggaatct
    30481 atacctaatg ttcatttttt acaagaccaa gttgtaaatc ttattgtaaa aaataggaaa
    30541 atattgggag ttgttacagg aatgggaaat aaaatttatt gtaaagcggt aattattacg
    30601 tcaggcacat ttctgaacgg taaaattcat attggagaaa aaaatttttc cggcggaaga
    30661 atcgcagaga cttctgtttc aggtctaaca gaaataatta atcgtttaga aattaaatcg
    30721 ggcagattaa aaactggaac accaccacgt atagatagcc gttcaatcga cttttcaaaa
    30781 ctaactcaac aagttggaga caaacctcca ggacagatgt cttttcgaga aataattgat
    30841 aaagaaaatt tctatcaact tccttgctac attacatata ccaattcatt cgttcatgaa
    30901 atattaaaaa aagggttcga tcgttcacca ttgtttacag gacgcattca aggggtagga
    30961 cctcgatatt gtccttctat cgaagataag atacaaagat tttcttctaa ggattctcat
    31021 caactttttc tcgaacccga aggaaggaca acagttgaat attatctcaa cgggttttcc
    31081 tcttcattac ccgatgaaat acagatagaa gcacttcatc gaattatagg cttagaaaag
    31141 gcacggatat ttcgtccagg atatgcaatt gaatatgatt atttctaccc ttctcagctt
    31201 aatgataatt tagagtcaaa aatcatcgaa aatctattct ttgccggaca agtgaatgga
    31261 actaccggtt atgaagaagc tgcagcccaa ggtttaatgg caggaattaa tgcacatttg
    31321 aaatcgcatg gcttgaaaaa atttacttta tctcgatccg aagcctatat aggagtgtta
    31381 atcgatgatt taatcactaa agaattgaat gaaccatatc gcctttttac ttcaagagca
    31441 gaatttagaa ccttactacg tcaagataat gccgattttc gtcttacacc tttgggttat
    31501 tcaataggtc tggtttctaa ccaacaatgg ataaatctct gtgataaata tcgaacaatc
    31561 caaaaaattt tgcaattttt agaaatagat aatattttac agttatataa ttataatagc
    31621 gataaagagg cactgcctca aaataatcct attattaatt ttttagtaca gccgggcgtt
    31681 tctatctatc aaactctaaa caattttcca aattttaaac aatatgttga agatttacat
    31741 gctaacgaag aagaaatcga agaagcagaa attattatta aatattatga atatattcaa
    31801 aaggaaattg aagtagcaaa tagaattaag aaattcgaaa atttgtatct ttctccttta
    31861 atcgattata gtttgtttcc atcattaacg attgaagctc gtgaaaagct taataaaata
    31921 aaaccttcta cattaggaca agcatcacga ataagcggcg tctctccatc cgatctttcc
    31981 tttttactaa tctacttgaa taaaaattat agttcataga aaatatgcag tatgtaattt
    32041 gtcctatttg taaaaacgaa aaagtgaatc gaattctcgt aactaaagac tttttcctaa
    32101 gcaatgaaga atttgaaata tgggaatgtg aattctgcaa aataagattt actcaaccaa
    32161 taccttcaga agaaaaaatt caagactatt ataaaaagga agaatatttc tcacattata
    32221 aaaataaaaa aagtctatct tattatattt acaacattgc cagaaaaatt aacttaagaa
    32281 gaaaatacaa gtttataagt aaagacttgc aattaaagat tggacgagtt ttagatgtgg
    32341 gatgtggatc gggcgatttt ttaaattatc tctttagtaa aggatgggat tgcgcaggag
    32401 ttgaaccgga taatgactca cggcattatt tgacaaagat gaataaattt ccagtttatt
    32461 cttcattaaa tgagatagag tcggcaaata gcttcaactt aataacattc tggcatgtac
    32521 ttgagcatat tcatcacccg gaagacgcta ttcaaaaaat tatcaatctg cttaataaag
    32581 aaggtattct ccttattgct gttccgaatt acgattctcc agacgcagaa ttttttatgt
    32641 cttactgggc agcatgggat gtccctagac atttatttca tttcaatttg cttagcctaa
    32701 gcaaattatt aaaaataaac aatcttcaag ttataaaaca ttctggctta tattttgatg
    32761 ttttgtatat ttcctattta tcccaaaaat atcttaataa gttaatacct tgtattacag
    32821 gaagttttat tggatttcgt ttaatgctaa aatcgaagac aatgaacaac ccatcaagta
    32881 ttatagttgt agctaaaaaa caatgaagaa agcacattat aagatatcga gattttcttc
    32941 caaatctatc gtaataggca taaccctgct acttttggca ggaatctttt cgattatttg
    33001 gttcaacaat tatttacaat ggaaaaagtt tcagaaaact atttataatt cctataatca
    33061 aatatcaaat ttaacttatg aattaaaaaa tcaacttgat tcaaataata attatgcaaa
    33121 tattacgaac aaactgaaac aaataaaaaa ttccgatgaa ttacaaataa taatctataa
    33181 aaagccagat catcttgtat attggcataa taatcagtat ttgattaacc taaatgagtt
    33241 aagatttgcc gaggatggtt ttataagtaa taagaaaggt tcatttttat atacttgcga
    33301 ttctacaaat gaattcattc tgatggttgc atttccagtt tggattgaat ttgccattca
    33361 gaacgaaggt tttcaaccag aaccttcatc tatattcaaa aaatttggat tgttttcatt
    33421 atgtccaccc gcaaatgaag gttttccaat atataattcg caaggcaaag agattttctt
    33481 tgttgaattc aacaatccag tacattatta tccttggaag tatttattaa ttggtacatt
    33541 aattattatc ggattctata ttactgtaat acaattattt aatttaatat ttaaaaaaga
    33601 atatcagata aatattttca ttatattatc aatattcata ttattttgct tattttacaa
    33661 attaggtaag tattttgaac atgaatattt tataagttat cataattatt tggttatttt
    33721 taataaattg atttcatatt cagaattact attttttagc atattatcag caatatttat
    33781 caagaatcta tgcaaaatat tggaaaatta taataaaagg aaaattcaaa aatattcaaa
    33841 gattcaattt agtacaatta taatattttt agtgtatata atcttattaa ttgtttttta
    33901 ttatgtaatt gatcaaataa taatcaattc agatatttca tttactccag ctatgttctt
    33961 cgatattact tatataaatg gagtatcttt gatttcactc tttgttttgt tatatattat
    34021 actatatatt acaattaaaa tagttaacat atttcaaaac ccaatcatat cgaaatatca
    34081 tccgtatctt ttaattattc taattctgtt agttacaatt tcgtttatct cgaaaatatt
    34141 tatcgttttt actttactaa ttagcatagc ttcaatagag gtcgtggtaa ttattaaaaa
    34201 ttcaatacac ttcgatataa atttattaga gaaatttctt tatgttttga taataagttt
    34261 attcttaata ttatatttaa tttataattt agaagaaaaa gaatatagca agaagcaact
    34321 tattttgatg aatatcgaat caatggaaca aggcgatcca attacagaat cattatttgc
    34381 agatattgta tccgaaattc aatatgattc attgatattg aatttaatcg ataattctgc
    34441 ttataatcct gtgcttgtag aaaattatat acatgaaaaa tattttagag gattttggaa
    34501 tcaatttcat ataacaataa ctatttgtaa agaaaatgaa cgcataagaa taacacctat
    34561 attggaagaa ataagttgtt atgaatattt taatcattta tttgatttaa aaccggattc
    34621 aatatatgat acatccttta taaaaatttc tgattatggg caccgtttga atgggtatac
    34681 tggtgtaatt tcttttccac acataccctt taaattattc gtcgagttgt tacccaatcc
    34741 atctagtaat gcaacttttc tttatgattt agtaacttca caggatttaa aatcaagaat
    34801 caatctcgaa aattattcat ttgcaaggta ttttaataat aaattgattt cattttcggg
    34861 aagtgctgta ttccctgtag atttaccaaa gtcattcatc attaatgaaa aagatgaatc
    34921 atctatcatt gaggtgaaat caggaaagtt tcattactct attttaaaat cgaagaataa
    34981 atcgatatat attcttgcta acgaagttaa aagaattaat aattttattt cattccttgg
    35041 attattttta ataatttata caatctgggg tattgtttat tttttatgga atctaaaatc
    35101 aaaaaatttc tatttcaatc gaattggtat taagtttcaa attgttataa ccattatttt
    35161 attggtgtca tttagtatta taggatattt tgtatataat ttgtttgatt ctaattataa
    35221 aaaccaagta aaagccaaat tagtagaaac aatttattct gtttcatcgg aatttgcata
    35281 taaattaata gaagaaccta atttcttata taaagatatt gatttagaag catttattat
    35341 aaaattatcg aacatttttt ttactgatat taatttgtat gatatcaatg gaaaacttat
    35401 aacaagtacg agtcctaaac tttttcattt tcggatagtt gaaccactaa tcaatagaac
    35461 tgcatataat gtcttaacag aaggacaggc aacaacatat cttcaagaag aaaaagttgg
    35521 taattataat ttcttatcgg gctatattcc tttaagagat aacgataata ctattgttgc
    35581 ctatataaat ctacctcaaa tttcgagaat ttcacaacag caacaacaaa gggaagaata
    35641 tttatctacc ttaattagtg tgttcatatt ccttcttatc ttctcattgc ttattgcatt
    35701 gttcatagca aatattatag tcaaaccatt aaatgttctg agcgaatcac tttcacaact
    35761 acgattaggc gaatcgaata taaaaattcc tattgtccga aacgatgaaa ttgggctttt
    35821 aattgaaaag tacaataatt tagtagacca attagaaagt gctatcaacg aactgaaaca
    35881 taaggaacgt gatttggcat ggaaagatat tgcaaggcaa atagcgcatg agattaaaaa
    35941 tcctctaacg cctattcgtt tacaaataca gttcctcagt gcctcattta agcctggcga
    36001 ctctaattgg aacaaaaaat tttatgaatt ttcaaatctg ttgttagaac aaattgattt
    36061 actctctcaa ttggccgatg ctttttctga attaggccac ttgagcgaaa gtaaacaagt
    36121 ccaggtgaat ttaatcgaaa ttgttgaaga agccgcaaat atagtcttga caggatttag
    36181 tggcgagttt attcatcatt ttcctgatta ctctacaatc gttaaagcag accgaaacca
    36241 acttttacga ttattcatca atattctgaa gaatgctact caagctgttc aagatcagga
    36301 aaataaatta atagaaataa aatatttact cgattacgat catgtaatag tttcaatata
    36361 tgacaatggt aaaggaatga atgaagaaga gaaaaaaaag atttttacac cctattttac
    36421 gacaaaatca tttggaactg ggcttggact ttacatctcg aaaaatataa taaaggcaca
    36481 cggaggagaa atcacttttg aatcgtcggt tgaaaatgga acgacctttt acattaaact
    36541 gcctatgttt tcagaataaa tagactgaaa tccattccat aatttgttcg atttcacaaa
    36601 aggagattat ctttgccaaa attttacact tgtcgaaatg gatatgataa caataaagat
    36661 agtaaataat tctcatcatc ctctaccagt ctatcaaaca atgggtgcat caggtatgga
    36721 tctttgtgca tatttacatg aatcagtaga aatcctacca ttacatcgca atattattcc
    36781 aaccggcttg tttgtcgaaa ttcctgaagg ttatgaaggt cagattcgac cgcgtagtgg
    36841 cttagcaatc aagcatgggg ttacggtttt gaattctcca gggaccattg attcagatta
    36901 tagaggagaa attcagatta ttctcgttaa tctttccgat attcctttta ttgtaaatga
    36961 tggggataga attgcgcaat ttattttgat gaaaatcgaa aaaattatat ggcaaattga
    37021 attggaactt ggggcaacga caagggcagc gcgtggcttt ggccatacag gactttcgtc
    37081 taatcataaa taatttgata tataaaattt atcccttaaa aattgtttat aaatgaaaat
    37141 atttaatatt aatttgttct tgatatattt tgctttacta tgtgcttcaa atgcatttgc
    37201 acaaatacag gatacattgc caaacaaatt cattggtgaa aatatccgaa tagacgaaca
    37261 agcaaaatat ttagaagctt taaaattcgt attgcagggc gatgatagta ctgcagctca
    37321 gattttagca ttaatattga tgaacaaccc cgataatcat actgcagcct tcgaattgtc
    37381 aaagatatat tacagtcaag gaaattttta tgcttcagca gtattaatgg aaaatgcagt
    37441 gaaattaaat cccaataatt attggtattg gaaacaactt ttggactcat acgaagcaac
    37501 aggcaatgaa gaaacttata tgaaatattt tgcattgttt atttcgagat tccccgataa
    37561 gcctgaggat taccggaaat acttagatgt tctgattaaa aatcaaaaat acgacgaggc
    37621 aattagtttt attgataaac ttattagtaa aaatattgta aacgaagaaa taattataaa
    37681 gaaatttgag ttattattaa aaaaggacaa gaaaaaagat gcgatctgtg ttctaaataa
    37741 ttatttaaca aaatatcctt gttctgaatc aattatgctt gttttatcaa attactattt
    37801 tcaaaacaaa aaggataaag atgcgttgag tattttggat gatctcctaa attgcgatac
    37861 aaataatgaa attgcctcac tcacattagc cgaatattat aataaaaaag gaaaaaacga
    37921 taaagctttt aaatacctac taaaagcttt caataatcca caaattactg ttaataataa
    37981 gattcagtat ttgcttaatt tctattcttt ggacaatata aaggcttcag atactgcaca
    38041 aattttgaaa ttagtaggta ttatttccca agtgcataac caagaaccta actccaaaat
    38101 tcttgcagga aatatatatt ttgtgtcgca atattatgcg gaggctgcta aagaatatga
    38161 agcggctata gatttgaaag tgaataatta tgaagtctat gagaaattat tgttatgtta
    38221 tgcagcattg gaaaataatg acaagctaaa atcactggct gataaagtaa ttgagttata
    38281 tccatatcaa cccttaccat attattttag cgggttcgta tcattcattc aaaagaagta
    38341 taccgagtca ggtgaattat tggaaaaaag tttgaaatat tcgggccgaa acagcaacct
    38401 cacacaattt ctctatcgct tacttgctga agttaactcc aacttacagg attatgataa
    38461 atccgattac tatttcgaat tggcattgca gtcagatagt agcgacgcaa cattattgaa
    38521 taattatagt tattccttag cagagagaaa aaaagacctc gagaaagcgt taaaaatgtc
    38581 ggaaaaagcc ttatctatta atccaaaaga accatcatat cttgatacat acggatggat
    38641 tctttataga cttggacggt atgatgaagc aatagtttac ttgaagaagg ctgtcgaaaa
    38701 gacggataac acaaatgatg ctgtattatg ggaacacctg ggagatgctt attataagat
    38761 tgggaatatt gatgaagcat taaaaaattg gatgattgca tcagaaaaag aaggaagcag
    38821 cgaaaagcta cttcaaaaaa ttaaagataa gatgctttat gaataagaat agttttatac
    38881 tcttgatatt atttagctgg ttacttataa ttacttcatg caaagtgaaa gaaaaaccaa
    38941 tatctacacc ttatccgcaa acttttcgat atcctatttc agactatttc aaaatgcata
    39001 cacctcataa attcttaacc tgcaaagcaa atgtaaatta ttataaagat aagcaagatt
    39061 attcattcta tgttaactct cgtattattt acgattcggc aatatggctt tcggtatcac
    39121 caggcttagg aatagaagca ttgagaatca tgctcacaaa tgattcaata ttttatttag
    39181 acagaacaag acataaatac attacagaat cattacaaat tataagaact aaatggaatt
    39241 tgccttttga tataaaagta tttcaggcta taataactgc ggatttttta agacaatcgg
    39301 attcgattat ttatacatac aaaaaaacag ataaaggctt acgtgtaata aataataacg
    39361 ctaattatat tgattcgatg tttccattga ataacccaaa aattatcgat gtgtatctca
    39421 atgaaaaaaa acctgttaca acatcagtga atttcgatat aaattcatgc aaaatttcta
    39481 caataacaat taattataag gagcataaat tgattgataa tgaatatctg ccaacaaatt
    39541 ggaacatata tttacaaggc aaagaatcta aaaaaataga tataagtata gataaatggc
    39601 aaaaaactga taaagttgtt ttgcattttg ttatacctcc agattatgaa tccttaatca
    39661 tctattgaaa aattgattat taagatgaat gatttattaa aattattgat aatcatttta
    39721 atattagttc caggaaagag catagctcag aaatcgagaa ccgatcttga aaatcaaagg
    39781 aaattactcg aacaggagat caatgaaaca actcaaaaaa taaaaaaagc tcaaactaca
    39841 caaaaaaaca tcaccactga aataacactt atcaataaga atattaatga taggaaacgc
    39901 ttgattgaat cattgacgaa ggaaattcaa atagttgatc ttaatttgga acaaacgaca
    39961 aaagaaatca aacgttatga aaaaatgctg gaagcaaata agaaagagta tgaaaaatta
    40021 ttgttaaatt ttcaaatata tacaaatcaa ccaaacgatt tattacagtt tttgtttgca
    40081 tctaagagtt ttaatcaggc attattacgt ttaaagtatt atcgccaata taaaaatttt
    40141 ctacaggaac gcatgttgat gctcaaagag atacaaacaa gattagaagt tcaacaaaag
    40201 gaattatcac agcaaaaaca aactaaatat caactacaac aattagaatt agagcagaaa
    40261 aacaatcttg aagtcacaca aaagaaaaaa aatcgtttac ttcaagatgc aaaaaaagat
    40321 gaggcaaaac tgaagtcatt attggccgaa aagaagaaag cggccgaaac tctaaatgct
    40381 gccattagaa aattgattga agaagaggca aagaaagctg cagcaacttc aaataaatct
    40441 ggaaacaaag aatataaaag ttctgacggt tttgtaactt tttattcggc tgaagatttg
    40501 aaactttcag tagaattcga aaagaatcaa ggtaagttgc cttggccaat cgaaagagga
    40561 ctaatctcag aatattttgg tgaacatcca catccagtac taaaaggaat aaaagtcaag
    40621 aacaatggcc ttaattttat ttgcccgcca gagacgccag tttattcgat agcaaaaggt
    40681 aaagtcagta aaattatgga aattccaggt atgcgaaatg tggttattat cagacatgga
    40741 gaatttctaa cagtttatag caatcttaag aatataatgg taaaggaagg agaaatgatt
    40801 gcagcacata ctaaaattgg tactgctagt gagattaaaa atgaggaaaa cgccgaattg
    40861 cattttgaac tctggaaagg gaaaacacca caaaatccat tgccctggct aagtagtaaa
    40921 aattgaacta aaattcgata tccttctgtc ttaaaggtaa atttgtatca atcatcttag
    40981 caattaaaga aagaaatttt tgttcttcat caaaatgaaa ttttacatta atcagaatat
    41041 aatatagtgg atattgattg agaatgcctt cgatttcctg atttcctcta atccgcatga
    41101 ggtccttttc tgtggtaagg attattttat tagttcctat catgttttcg aaagttttaa
    41161 tgattctaat tatatcgttt tttgaataaa tatgatgatc tggaaacgag atatgctcta
    41221 ctctataatt atttaatttc aaatgatcga ctaaaggatt agcatctgct attccggtga
    41281 aaagaagaat tctactggtt tttggatttt tcttatatat attgttgctt atggcaggca
    41341 aaaaattaga atatttataa tacgtaaaaa aaagcttttg ataaggcaac ggctttattt
    41401 catgttcgac tatattttta agcatcattg ggaatatctt tgctgtttta gtgacaataa
    41461 tcagatcggc tcttttataa cctcttcttg attctcttaa actgccaatt ggaaatagat
    41521 tatccttggt ataaagatga aaaaaattcg ataaaagaat atttattgca ggacgtacag
    41581 aacgatgctg aaaagcatcg tcaagaatta tgatttgggt ttgtggaaaa ttatttagaa
    41641 tctcttgtat cccttttacc ctattcctgc tcacggctac tacaatatgg ggaaatttcc
    41701 tttttatttg aagtggctca tcaccagctt tttgtgaatt atcgtcgatt ttgataatct
    41761 gtaagcctct tgatttccgt ctgtatcctc tcgacagata aaccagatta tactgtttat
    41821 aaagattttg aatcaaaaac tcgcaatgag gagttttgcc agtacctccc atgcatagat
    41881 taccaatgga gataataggg atatcgaaag catacgattt gaatatgcca acattgtaaa
    41941 aaaagtttcg aaagcttgta ataaagccat aaatgaaggc aaatggcaaa agtaagagtt
    42001 tcaaaatatt catgagctaa aaatacgaag aatttatatt taaacaaaaa taaatcaaat
    42061 tctatgaaat ttgaaataat gtattattaa gagtaatgca gaattgaagc atatttcata
    42121 attttgtaaa aaagaaaatt caatgataac cgttcgtgat gtattgcaag tgctcgacca
    42181 atatgcacca ttcgtctatc aagaagaata tgacaattca ggatttcagt tgggggataa
    42241 ggatgaggag gttcatggaa tacttctttg cctcgatctg actacagaaa ttattgatga
    42301 agccatacaa aatcgattca atctgattct tactcatcat ccttttttct tcaaaggcct
    42361 tcgttatctc gacttttcaa agtccgaagg aagaatcata cgaaaactta ccgaaaatag
    42421 aatttctctc atttcctttc atactaattt cgataatgtg gcaaaaggaa cgaatgaagc
    42481 acttgctaac aaactcggat taaaagatac tatgatttta tgtcctaaga aaggagcttt
    42541 gagaaagttg gttacatttt gtccagttga tttttctgaa aaagttcgtt cggctttatt
    42601 cagtgtcggt ggaggatata ttggaaatta cgatagttgc agctttaatg ccgaaggata
    42661 tggtactttt cgagctcagg agggttcaca tccatacgtg ggagaaatca atcagataca
    42721 taaggaattg gaaactcgta tagaagtgat tttcccagtt cacctaaccg acacgataat
    42781 acgattatta ttggaagttc atccctatga agaagttgct tatgatatta ttcctttaga
    42841 taatgtttat acatcagtag gagaggggat tatcggaaat ttacctcaga attttgaact
    42901 tatggatttt ttggcttttc taaaatcatc attagaatgt aagtcgattc gatataatac
    42961 cttaagcaac aacaagataa tttctaaagt tgccttatgt ggaggttctg gttcgtttct
    43021 catcgaagaa gcagtaaaat tgaatgccga cgcttttgta acttcagatc tcaaatatca
    43081 caattttctc gattcagcaa ttgaaaatat gctacttatt gatgcaggtc attatgaaac
    43141 agaaattgga ggcctcaacc ttctgaaaga atttctctta caaaaatttc ctaattttgc
    43201 agtggaaatt tcacaaaaag gattgaatcc tgttaattat ttgtgatata ataagttgca
    43261 ctgtaatatc ttaatcagga aaataaattt atggacacaa acgccaacct tcagaatcca
    43321 caaacagaag agaatgtcga actttcaatc gaacagaagt taaccaatct ttaccgattg
    43381 cagcaaatag aatctcagat cgaccgcatc agaattataa gaggtgaact gcctcttgag
    43441 gtacaagatc tggaagatga aatcgcaggg ctcgaaactc gcattgataa ttatattcaa
    43501 caaattgccg agtacgagca aaaaattctg gaaaagaaaa atattataaa agattgccaa
    43561 gctcttatca agaaatatga agatcaacaa atgaatgtac gtaataacag agaatacgat
    43621 gcattgtcga aagaaataga atatcaacag ctcgaaatac agctggcgaa taagcggatc
    43681 aatgaattta ccaatgaatt gaatcattta aaggaagaaa ttgaaaaagc tcagaagctc
    43741 gtcgaagaaa ataaaagtga attggaaatt aagcgaagtg aattggctga tattatagca
    43801 gaaaccgaaa aggaagaaaa agaattgctt cagaaagctg aaaaatgcag gaaactcatt
    43861 gaagaccgct tattgctggc ttatacgcgt ataaggaaaa atgccagaaa tggcttggca
    43921 gtggtgcatg tggaaagaga tgcttgtgga ggatgtttca ataaaattcc tcctcagcat
    43981 cagttagata tacgtatgca taagaagatc atcgtttgcg aatattgcgg cagaattctg
    44041 gttgaccaag ctattgtaga tagcgtaaat cagtcttgat aaaatcttgt attcagtcat
    44101 gcaccttctg aatataagaa ggtgtatttt tttaatcgtt gctgataatt ctatcattaa
    44161 ggacctgttt atgatacggc attattcatt atttttgttg ataataatat tctcaatcat
    44221 tctttctata tcgaaaattt ctgcacaaaa tcaatatttt tttgatatat acaccacaag
    44281 agcattagaa ttagcttctg aatttcgatt cgaaaaagcc agggagcaag tgaatatttc
    44341 taaaagcctg aattctaaag cgaatatacc catttgggtt gaaaactata tcgatttcat
    44401 ttatttactg aactctgaag atgataaaca ataccaaaat ctcaaaaatt caaagaaaat
    44461 tcgtctaaaa tggttggagt ctggagatca aaactcacct tattataggt attgtctggg
    44521 tcattttcat tttcaatggg ctataatcca tctaaaattc aaagattact acacggcttt
    44581 taaagaagcg aatactgcat atcgtttgct tgaaaagaat tatcgtcttt atcctcagtt
    44641 tccaccttct ctttctgcat taggtgtttt acatgttttc gtcggctcgg tacccgatca
    44701 atacaattgg ataaaaaatc tactcggata caaaggaact gtcgaacaag gtctgagtga
    44761 actcgaaact gcattggaag aatctatgca aaataatgat ttattctggc tcgagaaaga
    44821 aacagtattc tacttgagtt ttatttatat caatcttgat aaaggaaaag aaaaatctct
    44881 ctcttttcgt aacaaatatc taagtcaaaa agatattgag cttgagaaac tcgatcctat
    44941 acaggctttt gcattttcga aagtgtcact gtttacgggt cgaaacgatg aggttattcg
    45001 tatccttcga tcgagaaaat tctatcccga tcaaactgaa tttacttttc tcgattattt
    45061 actggctaat gcttaccaga ataaactctc ggatagttgt ttgatatatt atgacaaata
    45121 tttaagctat cctacggtaa agaatttcaa aaaatcaagt cttcagcgaa aagcatggca
    45181 atatttaata aaagaagata gagaagaata ttatttattg atgaagcagg ttcttaaaac
    45241 aggtcaggaa gaggtggatg cagataaaca ggctcatttt gcagctacaa agggcattat
    45301 accaaacgtt ctattattga aatcacgact tctttttgat ggtggatatt acaatcaagc
    45361 cttgaatctg ttgttagaga ataaagataa attgaatact ttgccaatta tcgaacagac
    45421 tgaataccat tatcgcttag gcagaattta cgacgaaacc cataatttag tgatggctaa
    45481 actcgaatat cacaaggcaa tcgaattagg taaggcgctc ccacaatatt atgccgcaaa
    45541 cgcatcgctc atgatggcga tgatttatga aaaacaacat cagtatccac tggccaggca
    45601 tcattataag atgtgcatgg atatgccttt cgaagagtat agaaatagta taactcaaaa
    45661 agccaagatt gggatggaaa gaacaaagaa aatgaagtaa attattgaat attaaaatat
    45721 tttaattatg atttcaaata attatgaaag aatgctcaaa cttgtagatg aagtttttgc
    45781 tacttcgagt gatcctaacc aaataagagt tactcccgaa gtaatggatc gtttgaaaga
    45841 gatgcatcca tcaacttttg cagagtatag cgacagcaat ggtcctgtgg cgtggattat
    45901 gattattccc tctacactcg aacttatgaa ttgtttcgtt aaaggagaaa tatctgaaaa
    45961 tgaacttttc gaacttacac cctcgaatat atcatatgaa gcactctatc tttgttctgc
    46021 gttggtttta gaagaatatc gacgtcaagg aatagctaag cgtcttattc ttcaagctgt
    46081 tgagaatata cgccaacaat ttcctatcaa ggcacttttt gtgtggcctt tcacaaaaga
    46141 aggcgaattg attgccgaaa atattgctcg taaagtctcc ttaccacttt acaaacgatt
    46201 ggaaaacaaa taattctgta acataaaata atttttaatc agcacattga aaattccgaa
    46261 ggatctaagc cgttaggtag tttcgaaaat aaatgtcaaa ttcacaacca agaatttggc
    46321 tttatacttt attaatcgat tcgaagacag aagattattt gaatagcagt ggtagctata
    46381 tggtgattat attatataat ttatggatta ctgagatggt ccaatgagtc tggacaatat
    46441 ttttatcaaa gttaatttat tatgtt
//
LOCUS       DAOORU010000005        38365 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_1225_length_38365_cov_5.864892, whole genome shotgun sequence.
ACCESSION   DAOORU010000005 DAOORU010000000
VERSION     DAOORU010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 38365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38365
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1225_length_38365_cov_5.864892"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(941..1885)
                     /locus_tag="PLF20_00890"
     CDS             complement(941..1885)
                     /locus_tag="PLF20_00890"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOX79598.1"
                     /translation="MDFPQLIPLHMLANLLNAKLEGDPQTLVKGLNEIHKAEPGDLTF
                     VDHSKYYQKAFNSQASVILIDQKVECPAGKALLIHPDPFEAYISLVRRFHPFQPSSSM
                     ISPSAIIGEGTIVQPGVFIGNNVVIGKNCIIHANVSIYDHTIIGDRVIIHSGSVLGAD
                     AYYFQNHEGSYRKLESCGKVVVGNDVEIGALCAIDKGVSAETYIGDGTKMDNHVQIGH
                     DTRIGKNCLIGAHSAIAGVTTLEDNVVLWARVSLNKDLVIGKGAVLLATSSVSKSLEG
                     GKTYFGAPAIEARIKWKELAALKQLPDLLQKLHSLLKD"
     gene            complement(1931..2497)
                     /gene="efp"
                     /locus_tag="PLF20_00895"
     CDS             complement(1931..2497)
                     /gene="efp"
                     /locus_tag="PLF20_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOX79599.1"
                     /translation="MATTADFRNGLCIEFNGEPYTIVEFQHVKPGKGGAFVRTKLKNL
                     RTGRVIPNTFTAGVKIDILRIERREYQFLYADGEDYYFMHNEDFEQISLPKDLIDAPQ
                     FLKEGMICEILFLADNEQPISCELPTHVELEVTYTEPGERGNTATNALKPATVETGAI
                     VKVPLFINTGEIIRIDTRIGDYVERVKD"
     gene            2901..3416
                     /locus_tag="PLF20_00900"
     CDS             2901..3416
                     /locus_tag="PLF20_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOX79600.1"
                     /translation="MDIIRSRRSIRRYIPDEVKNEDIEGMLEAAMYAPSAGNQRPWEF
                     IVVRNEAMREIMSKTGPYSFHAAKAPVVILVCGDSLHETHKNYWPVDCSAATENILLE
                     ATARNLGSVWLGVYPRQERMEYLSHLFQLPEHIHPFAMVCIGYANEKPPARGFYDPSK
                     VSWEKFGNKKV"
     gene            complement(3439..4461)
                     /locus_tag="PLF20_00905"
     CDS             complement(3439..4461)
                     /locus_tag="PLF20_00905"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HOX79601.1"
                     /translation="MDKKNFLKDVIQHVDITAFDGRPIIDAMRGMSFSSRDTAQAADI
                     FNLMLSDEDCSIILTLAGSTSAGGCMQVYADMIEYNMVDAVVATGASIVDMDFFEALG
                     FKHYKGSPYVDDKELRNHYIDRIYDTFIDEDELQACDDTIQQIADNLEPRPYSSREFI
                     YEMGRYLTQKAKKPGSLVQIACENNVPIFCPAFSDSSAGFGLVKHQWNRPDRHVSIDS
                     VKDFLELTQIKIKADQTGLFMIGGGTPKNFAQDTVVCGEVLGFNLPMHKYAVQITVAD
                     VRDGACSSSTLKEAASWGKVDTVYEQMVYAEATTVVPLIVNYAYLTGYWKNRKRYEWV
                     KIFDGK"
     gene            complement(4562..5953)
                     /locus_tag="PLF20_00910"
     CDS             complement(4562..5953)
                     /locus_tag="PLF20_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HOX79602.1"
                     /translation="MKNRYIDLIEQTFEFPKEEFNVIDNELYWNNIPLMDIIKQYGTP
                     VRLTYLPRISEQIQKARINFNVAMAKTEYNGEYNYCYCTKSSYFSFILEEVLENDVHI
                     ETSSAFDINIIEELYDTGHLKKSNYIICNGFKRPQYIENIVNLIHEGFENTIPVLDNK
                     FELDKYIEAGLKRKTKIGIRIATEEEPQFQFYTSRLGIRYNQIIPYYQEKIQNNPLFE
                     LKMLHFFINTGIKDTAHYWNELLKAVNLYCELKKICPELNALNIGGGFPIKNSLEFNY
                     DYEYMAEEIISQIKNTCMQHGVPEPDIFSEFGTYTVGESGAILFSIVNQKQQNDRELW
                     NMIDGSFMTTLPDTWAISQRYILLAVNNWDVEYQRVHLGGLTCDSQDFYNAEAHSNAV
                     FLPKFNDKEQYIGFFNIGAYQESLSGYGGLQHCLLPAPKHVIIDLDENGEYYTKLFAK
                     EQSYKSMLKILGF"
     gene            complement(6226..7449)
                     /locus_tag="PLF20_00915"
     CDS             complement(6226..7449)
                     /locus_tag="PLF20_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOX79603.1"
                     /translation="METIHNDLSQVNLAETLAPQEQLKPAARTIKRFSIGIPREISPD
                     ENRIALVPSAVQELVQNGHTVYLERGAGNNAKFTDVEFSEAGAKICETTEEVYQAEII
                     VKVAPPKNQEIEFLKPRQVLFSSVQTFIQQKNYFISLLQKKITAFGFEYVKDDSNAFP
                     VMRSMSEIAGTTSVLIAAEYLSHPVFGKGSMLGGFPGINPSEVIILGGGTVAEYAART
                     ALGMGALVKIFDDKIYKLRNIQYQLGSRIFTSIIQPKVLLKSLQTADVVIAALYSREG
                     RTPVIITEEMVNQMKKGSVIVDVSIDQGGCVETSRPTSHSHPIYQVNGVTHYCVPNIA
                     SKVPHTASYAISNVFSPLILKIGYTGGIEKYLTRDPGLRQGVYIFNGILTNKTIGEYF
                     TLPFKNIELLMAAFR"
     gene            complement(7531..8485)
                     /locus_tag="PLF20_00920"
                     /pseudo
     CDS             complement(7531..8485)
                     /locus_tag="PLF20_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009128829.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(9033..10118)
                     /locus_tag="PLF20_00925"
     CDS             complement(9033..10118)
                     /locus_tag="PLF20_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX79604.1"
                     /translation="MKSLVRILNCLMIPFVLSLILGFSSLAFGQNQPPFSPPSKNQNL
                     SPEFQSLPDKANWEEIMNELENLYMLFPAEEWNDEPMEEYLSDFRDFMQREAPPIPEN
                     LMEVKRHLEMAMRQLDEAIRQMPEFPRPDLKGWKFREHHLSDFHSYGFHHLPQGCPYG
                     TFRDDSLVMDTAGKSIKIIRKMIVGRDTLTKEIECIRMSTPNGRVDTLIIFKDGRKTI
                     DSIVWNRSRQRADLRTFRQTPQSWRYSERKPKSRTLYMVDLNSDDLNKLSHSALKPSQ
                     VKEPLDLDKLSVNKSVRGKIRLRFTTEQSGDIEVSVFDEQGSILHHEILKKFSGNYDR
                     IITLLPQPVYYLKIQQGKKVLQKKITE"
     gene            10253..12055
                     /locus_tag="PLF20_00930"
     CDS             10253..12055
                     /locus_tag="PLF20_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX79605.1"
                     /translation="MNKSVIRVVIILSGISLVSLVILQFVWFKNMLQMRENEFDRDVM
                     AAVAETARRLERDQTMFFVSNRLRMQALTDTILASTHPKNRTATINKTSTNMNSILPD
                     SLPPRRRVNLKHRQWVVQQNARGEIIVETENQLDTLTSMDMSVLFDPRFERMLPFANL
                     SADEYYNAIIRRQERFFREQRQMMDKFDSVFMRYQGRIISDRPKPRPQKLNPNPAFQK
                     QVEDLQSQTTLLSDALDRLIFEIKQLEMPARERLDVPASHEILKQELKKHNIDLPFEY
                     AIFDEERDTLVRSPRFNPLSSTKVYEANLFTDRFFQSADHIALYFPDRSTHLLRSMLW
                     IITGSALFTLIIVITFIISILVILRQKKISDVKSDFINNMTHEFKTPIATISLAADSL
                     VNARIINDPEKIHYFTRVIKEESKRMNTQVESMLQMALLEKKDFSLHLQPMDAHDLMA
                     QAVQNISIQIHKRGGEIHTDLQATNPIVEVDEVHFLNVLFNLLDNANKYSPQSPQIEV
                     NTWNGHHSLFISVKDHGMGMDRETRNRAFEKFYRKSTGNIHNVKGFGLGLSYVKAIIM
                     ASGGNIHIQSEPGNGSEFIIELPQINNDDESTID"
     gene            12036..12737
                     /locus_tag="PLF20_00935"
     CDS             12036..12737
                     /locus_tag="PLF20_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006847396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX79606.1"
                     /translation="MNQQSIKIFLVEDDLNFGAILKSYLELNDYEVIWVNDGKDAMTA
                     FYQGKFDICILDVMLPNIDGFSIAKEIKHFRPDIPLIFLTAKTLKNDVLEGFKLGADD
                     YITKPFDSDVLLYKIRAILKRHGQVSQKEEQREFQIGEYKFDAFLRIITHNDESRKIS
                     PKEAELLKMLCMNMNDIVPRELALKTIWGDDNYFTTRSMDVFITKLRKYLSKDPNVEI
                     ENIHGTGYRLLLKNS"
     gene            complement(12801..13766)
                     /locus_tag="PLF20_00940"
     CDS             complement(12801..13766)
                     /locus_tag="PLF20_00940"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOX79607.1"
                     /translation="MYNAVLETNYHFPGQTKIYYGKVRDVYFVDDLLVMIATDRISAF
                     DVVLPRAIPYKGKVLNQIAAYFLDATSDIVPNWKIATPDPMVTTGYLCKPYPVEMIIR
                     AYLTGSSWRAYLKGARQICGVPLPEGMKEHQPFPQPIITPTTKATEGHDMDITRNEII
                     QNGLIPEEDYLQIEEYTRRLFERGSIMAAQKGLILVDTKYEFGNRDGKIYLIDEVHTP
                     DSSRYFYAEGYQERFAKGLPQLQLSKEFVREWLMERGFMGQIGQRVPDMDDDFVNSVS
                     ERYIELYEKITGEKFVRTSLDNIEERIEKNVLQFLTEYKGINSLK"
     gene            complement(13824..14783)
                     /locus_tag="PLF20_00945"
     CDS             complement(13824..14783)
                     /locus_tag="PLF20_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOX79608.1"
                     /translation="MNERIISIESYSPLEIYGPNDQYLNLIRDRFPQLKIIARGDVIK
                     IIGEKSQIDLFDRLFGLLLVHYDEFGKITENDLFEIFSNEGEEEFNQVRPIEGNDILV
                     YGNHGLQVRARTINQRLLVESSQKNDMVFAIGPAGTGKTYTAVALAVKALKNREVKRI
                     VLTRPAVEAGENLGFLPGDLKDKLDPYLQPLYDALRDMLPTQKLLTYLEDGTIEVAPL
                     AFMRGRTLDHAFAILDEAQNATLNQLKMFLTRMGRSAKFIITGDITQIDLPPSQKSGL
                     VHATQILKGIQGIDFVFLDQRDIIRHRLVKDVIEAYGRDNPQH"
     gene            14885..15664
                     /locus_tag="PLF20_00950"
     CDS             14885..15664
                     /locus_tag="PLF20_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HOX79609.1"
                     /translation="MSIITLTSDWGLKDHYLSVVKAAILSRLPETIIIDISHQIPPFD
                     LNQASFIIRNCWKGFPKGTVHIIGINTEAGIKSPHTAVEMEGHYFIGADNGIFSLIFD
                     KQPERIIEIEIAQDSEYFTFSTRDVFVKAAVHLLKGNSIEDLGIPRNSLTPCASFQPV
                     ITQNAITGKVIYVDVYENVFTNISREMFQQVIKGRHFQIELGYIKNKINRISDSYGDV
                     PEGEIVALFSSTGLLQIAINHGNASGLLGLSIDDSIRIVFE"
     gene            complement(16073..18025)
                     /locus_tag="PLF20_00955"
     CDS             complement(16073..18025)
                     /locus_tag="PLF20_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOX79610.1"
                     /translation="MESRRYFIRILKKAIDNAMLALGLGLLLLMCHCANPVVPQGGPK
                     DTDPPKLLYAVPPNLSTHFTASHIKLTFDEYVVLKEINKKFLMSPPSLNLPEFKVKGK
                     SLEIDLKDTLRPNTTYSLYFADAITDLHENNPLPDFQYVFSTGAVIDSLTMTGSVVNA
                     FNLKPVENMVVMLYYANNDTLPLEQLPLKVPPSYAARTRKDGSFNLNYLRDEKYLLFA
                     LIDQNYNYFFDLAEEEIAFSDTLVRAFYIPPAPDTSFVPKLAVSMPDSLLHDTIAHQE
                     LKDSIQHMLFQDTLRQIQHLRDTNDTAPSDTLAKVVSQTKEYFSSLPDTLAKNDSTVV
                     RQPHLSFVSYRLFSFVEEDTVQRLLRRENTGNGQIFLNFKRSIEDFSYRLLPPFDSLY
                     QPLLEINRNQDSIKLWFPSCPFDSVKICIYTTGIQPDTLVLPLKRETIGTRSKKTKTE
                     SLMVETLSGQTDAFKPFILKFNHPVDSVKSDSLLLFTSKDTLWVKFEFADSLKRKALV
                     KYSLDYNSSYQLIIPSKTFVDIFGLANDSITARFTTRAADAYGSFHLKLISPASNNQY
                     LIQLLDEKENLVRQDIINDLVCEIDYGMLLPKKFILKAVLDCNRNRKWDTGNYRKKRQ
                     PEKVQYFKAPIEIRANWDLREEWILE"
     gene            complement(18028..18657)
                     /locus_tag="PLF20_00960"
     CDS             complement(18028..18657)
                     /locus_tag="PLF20_00960"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOX79611.1"
                     /translation="MIKATTCFPRAVEWIFAPFVITRMPRTGQCLYLTFDDGPHSQIT
                     PKVLDILNRFQVKATFFCLGKNIRAYPEVFQEIKNNGHNIGNHSFSHKNGWKTSNKEY
                     FKDIEACRELIPTNLFRPPYGRMRVSQVRVLSRQYQIILWTLLSGDYNRSLSSEEILR
                     TLLRRTRSGDIIVFHDNEKAFPCLIEVLPSYLEAMLEKGFTFDVIQSKL"
     gene            complement(18663..21851)
                     /locus_tag="PLF20_00965"
     CDS             complement(18663..21851)
                     /locus_tag="PLF20_00965"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOX79612.1"
                     /translation="MSFSFQKIDKILGWEIFFIAAAVYISTVEPTASYWDCGEYISTA
                     YKLQIGHPPGAPLYQLLGRLFSLLSFGDISKVASSINLMSAIASAFTVMFLYWSITLL
                     VSHFLSKNDEVRAENEYLILGIGLSGALAYLFTDSFWFSAVEAEVYALSSFFTAIMFW
                     AILRWERGSSDSHGIRWLILIAYLIGLSIGVHQLNLLTIPAIILLIYFHVRRPTVAGV
                     IGALAISMVLLTFILYGLIPEIPGLFARTELFMVNRLGLPFGSGSFFSASLIVTMLIS
                     GILFTHYPNHFFRGLFLMVSLLVLIIVMTGSKTLIEFIIRGFIVLGLGWGILYLMKHR
                     QVMNTILLSLSFIIIGYSSFLMIIIRANANPPINENDPKDALGLLSYLNREQYGNWPL
                     VYGADFTADIVDYKDGEPVYMKDYTAGRYVAKDSRKAAKPVYNSRMFFPRMYSRSYKH
                     IQEYKIWAGIPDDENYRPTFGDHLGFLINYQLNQMYWRYFLWNFSGRQNDSQGFSDKA
                     NGNWISGWNFLDKWRVGPLSHLPEHKKSRAWNRYYLLPLLFGLVGMVYHYKNDWKSLM
                     VVLVFFIMTGIAIIVYLNQYSPQPRERDYAYVASFCAFAIWIGMGTGAFASGMTKWIN
                     GRKSILLTTGLNLLCVTGVLAAQGWNDHNRSNRYATTQMAKAYLDSCASNAILFTFGD
                     NDTFPLWYLQEVENYRTDIRVCNLSLLSLDWYIEQMKRKVYESAPLPIQLDFSFYKQG
                     THDYIYFISDDDSLTDTLNLCSIFEQMSVEPQKFKYVIETDTIDYLPSNRFVLNIDKT
                     AVLNHGVIDSDQKDRIVDRMFFEIPGREFEKNTLIVLNILANNNWGRPVYFGLTGNTR
                     EFLGLEEYFRLEGMAYRLVPILMKSRHDRMGGIDSEILYDHLMNRSQVKMNNPEIYYS
                     ENHLKNCTLLRNIFRSLADTLLKEGEKELAVRVCDRCIVNLPNSVIPYDVFSLALAEV
                     YYGAGEISKGNEIMTVMMNQSSAELGYYFELLPNLLHRYEYEINLNLGILNYIAETAC
                     KFGQEEIGDKSKEAFEKNLKCYFTQIYQNFEP"
     gene            21972..22715
                     /gene="gpmA"
                     /locus_tag="PLF20_00970"
     CDS             21972..22715
                     /gene="gpmA"
                     /locus_tag="PLF20_00970"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOX79613.1"
                     /translation="MLKLVLVRHGESVWNKENRFTGWTDVDLSEKGVEEAHQAGKVLK
                     ENGFDFKVSYTSYLKRAIKTLWIILEEMDLMWIPEYKTWRLNEKHYGKLQGLNKIETA
                     EKYGEEQVKIWRRSYDIPPEPLSPDDPRNPRLDPRYAEIGDDAPLTEALKDTIARIVP
                     YWENEMSIKLRQYKEVLVAAHGNSLRGIVKYLKNMNEKEIIDLNLPTGIPYIFEFDDD
                     MNLQKDYFLGDEEEIRKAMEAVANQAKKK"
     gene            22873..23589
                     /locus_tag="PLF20_00975"
     CDS             22873..23589
                     /locus_tag="PLF20_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOX79614.1"
                     /translation="MPQQWVIPDTHGYLKTLRALLEEQIRPSKFDEIYFLGDFIDRGP
                     DSKGLIDYLIHLKDDGYRLRMLKGNHEEWCIRAYEEELQLKKSFLGLKKPNIQKAEWM
                     RFGGKETLKSFKVTNLRYFPSEYIEWMHQLEYYIELEDYYLVHAGFNFETDSIFDDRD
                     AMLWIRDFNVSPEKINHKIIIHGHVPVSHEFIDFTIRKKQYHFIPLDNGVYIKKKGFG
                     NLTAFEIKQQILLVQPSLDD"
     gene            complement(23688..26174)
                     /locus_tag="PLF20_00980"
     CDS             complement(23688..26174)
                     /locus_tag="PLF20_00980"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOX79615.1"
                     /translation="MDNNVNMPDYQQQEENIDFKQIFFKFLRYWYLFALAIFVALVIA
                     FLFNKYTTPTYEVSTTVLIQDKGNKEINAQALMGLGFMNSQQNIQNELGILSSYTLTS
                     EAVKVLNVDVTYYSENNFIVRELYRKSPFTVFYDSIHPQPVHLRFYVTILNNRQFKLE
                     TQGENIKLFDFENNKFIEEQQDGETKIKTIKLNQIYDFGTTISGPYYKFRVLLNYNFN
                     SKEHVNKTFFFTFNDLPSLVKEFHNIKIEPINREASILEIKAKGSQVEKTVDFLNTLT
                     SKYLERDLDQKNIIATKTIEFIDRELIGIRDSLSIAESQLEGFKKEAKIMELDFESQQ
                     VFQYMKELDKQKADLLVKNKYYNYLKDYVQSKNEMNDIIVPSAMGIDDPVLAELIKNL
                     TSLYEERSGLLLSSTPRNPAIIQIESKIANAKANLVENINNIVNTSNIALADIEKRIQ
                     DLNQQISQLPTSQRRLLNFEREFNLQNTLFTFLLQKRAEAQITKASNLPDNQVIDVAR
                     AEGKSPIFPKKSLNYLIAITLGLVIPGGYVFIKDYFNDKIIEKKDIEKITSLPIIGYI
                     SHNTREAKMVVAEWPKSSIAEAFRALRTNIIFRAQGKEKQTILITSDIVGAGKTFIAI
                     NLATVFAINDKKTLLMGADLRKPKLHQDFGLANTDGITSFIIGKHKIEEIIHPTKIEN
                     LDVILGGPVPPNPAELLASPKTDDLFDYLKKHYDYIILDTPPVGVVTDAFLLMKHSDQ
                     NIFIIRQGVTNKKIFGAIIKDIENRKLPNFSVLVNDIKMAKNIYGYGYGYGYGYGYGY
                     GYGHGYGYGYYSDDREVEKESAWKRIFKRS"
     gene            complement(26195..26986)
                     /locus_tag="PLF20_00985"
     CDS             complement(26195..26986)
                     /locus_tag="PLF20_00985"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HOX79616.1"
                     /translation="MKKSFNAYGGIFIQVLLLSMMMISCVPQKKIKYFQSLAKEDTLE
                     IFEFSPKPDYRLKVGDNLYIKVQSLDEKTYKFFNYEDMGRATNIGYSDANIYLTSYTV
                     SDSGYIHFPFVGKIFVSGLTVDEVSKKLQDIVDQYLVETTVIVKLVNFKITVLGEVKN
                     PGTYLIYQDKINIYEALAKAGDLTVFSDRNKVVLVRQDQNGAKLHRIDLTKKNFLESP
                     YLYLLPNDIVYVEPLRAKQYAFAAFPYDLLFSTITTTLLILNYFK"
     gene            27415..28803
                     /locus_tag="PLF20_00990"
     CDS             27415..28803
                     /locus_tag="PLF20_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOX79617.1"
                     /translation="MNTKKDMLPQTSDTKSSKKSGSKKIFVLDTSVILYNHASFYSFD
                     DNDIAIPITVLEELDNFKKGNEIKNFEAREFIRIIDRLSHKKNLTDWVAIDNGKGGSF
                     RVILDQPHEQKVQQIFGGYKNDHRILNAALTLKEIYPERKVILVSKDINLRIKAKALN
                     LQAEDFETGKIKDIENLDPGKKVISHIAPQVLSKLWTTGKCSPSEIGEKEPIPNYYYI
                     FQNASENENPTLAFYNPKSGNIERIDPQPVERIMPRNPEQAIALHAITNPNVKLVTLI
                     GIAGTGKTLLALAGALEQRRLYKQIYLARPIVPLSNKDIGFLPGSAEEKISPYMEPLY
                     DNLKFIKNQYSEKEREYKLFDEMLENGKIVIAPLAFIRGRSLSNICFIVDEAQNLTPH
                     EVKTIITRAGEGTKIIFTGDIYQIDTPYLDSQSNGLSYLVDKIHSHPIYAHVKLERGE
                     RSELANLASELL"
     gene            complement(29179..30255)
                     /locus_tag="PLF20_00995"
     CDS             complement(29179..30255)
                     /locus_tag="PLF20_00995"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOX79618.1"
                     /translation="MKNKRILIIILIVLLAIIAGWLIFGRSNTTFDKDEGKFAVEDTA
                     SISRILLADKRNHSVILERREDGHWTVNGEYPASKPVIDMFLQTIKNIRVKEPVPANQ
                     QKNVISLLAANAVKVEIYQWLPRIHCFGKQAWFTEKQSKVYYVGDATPDLRGTYMLME
                     GANMPFIVDLPGLRGFVSPRYSTRLEDWRDHTIFHQRLADIHEVTVEIPEMPEQSYKV
                     IHHDDNRLEVVSLATQQTVSGYDTLKVLAFMNSFNNIKFESFLNHVEDLDMDSLLASR
                     PFFVITLRDRNGKITTIRTFHKKPEHEVRDENGKLFPWDVDRLYAEVNDGKDIVLAQF
                     FTFDKILRPLGYFYIKPSEVSNIK"
     gene            30410..31180
                     /locus_tag="PLF20_01000"
     CDS             30410..31180
                     /locus_tag="PLF20_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX79619.1"
                     /translation="MNLFYRSFGQGPHLLILHGLFGLSDNWVSLGHRYAESFTVWIPD
                     LRNHGQSPHHPEMGYLTMVNDIFDFFELHQIEKSAIIGHSMGGKVAMLFATQHPEMIS
                     KLVIADIGPGPTPTPNLQKTIIETMSSIDLSTMKNRRQVIKQLQDTFTQRGLIDLILK
                     NIGRNSEGIYYWKPNLEAFYQNLDALAANIKFSDYYPNPTLLLKGEFSNYITEKDLTI
                     LYEHFPLMKEAIIHNAGHWLHDDNPQEFFEVTWKFLDS"
     gene            complement(31187..31966)
                     /locus_tag="PLF20_01005"
     CDS             complement(31187..31966)
                     /locus_tag="PLF20_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79620.1"
                     /translation="MPIKPHFKTLQLTVCFLFIVFILEDFTTPVLAQTSRYGKNKIYE
                     DLKRNWSVQWNIGPGLYFGDLSQYDNDPINKIFKESKLSSGFIISNKFISYLAIQGRF
                     IFNRYQTSNEIFNRKLKGYSYVIGANLQLDLVNLMAYPNEPHPDIYVYLLFGVGSTKM
                     RPSLYNLESGNKIELNTVQKKMEFMIYYGVGANVYLYKNWDLTFETIIQNIGTDKLDG
                     VIAGEPDDFIIYTSVGIKYNFPYFDLGNKYLYRSKRTLLRR"
     gene            complement(32140..32946)
                     /gene="tatC"
                     /locus_tag="PLF20_01010"
     CDS             complement(32140..32946)
                     /gene="tatC"
                     /locus_tag="PLF20_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOX79621.1"
                     /translation="MKKKSNPQKEMGFWEHLEELRWHLVRSIIAVALGATFAFMKRDF
                     IFDTVLLGPKSNDFITIRFLCSLGEKLGISALCLDNSKLSLINISMSGQFTQHIYISL
                     IAGLIIASPYVFYELWRFIKPALREEELRYSSIAITIVSCLFIMGIMFSYFLIVPLTI
                     NFLGSYQVSEVVRNQVSLSSYISTVVGVTLSVGIIFELPALAWVLSRLGIITDRWMRK
                     YRKVMIVVIFIVAAIITPPDMFSQIMVAIPLLILYEISIYVATLAAKNRK"
     gene            33053..33574
                     /locus_tag="PLF20_01015"
     CDS             33053..33574
                     /locus_tag="PLF20_01015"
                     /inference="COORDINATES: protein motif:HMM:NF021439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HOX79622.1"
                     /translation="MEENFRKKTAHDVARLMMAAARTAPKGRGRDSLFIALASDDEMQ
                     QIAKTMKIFAEEWETQFFARDARNLLHSDALVLIGTKIEPLDLRKCGFCGFENCSEKN
                     LHPNTPCAYNNIDLGIAVGSAVAVAAQHHADNRVMYSVGKTALYMGLAKPDVKILLGI
                     PLSIRSKNIYFDR"
     gene            complement(33571..33765)
                     /locus_tag="PLF20_01020"
     CDS             complement(33571..33765)
                     /locus_tag="PLF20_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79623.1"
                     /translation="MLHSSEAKKKAIELAKIVVSCCAKKVSSRLDDNTAEAILAGIFV
                     LKLREKNLVLPSEVKKLMRF"
     gene            complement(33776..34051)
                     /locus_tag="PLF20_01025"
     CDS             complement(33776..34051)
                     /locus_tag="PLF20_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79624.1"
                     /translation="MGTLISIDTGVKLGMAVFNFEGKLLFYGSHNYGDINRLKRNIPR
                     ILKRYETVKLLVLEGGGELAEIWLLEAVRQQAEVLMIHAQQWRKLFF"
     gene            complement(34060..34752)
                     /locus_tag="PLF20_01030"
     CDS             complement(34060..34752)
                     /locus_tag="PLF20_01030"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HOX79625.1"
                     /translation="MFRFKQFEIEDNQSTMKVCTDSVILGAWVAIGEAERILDIGTGC
                     GLLSLMCAQRSKAWITGIDIHFPSIEQAQENFKKSRWKNRLKAKHIALEDYEGNSFDY
                     IITNPPFFINSLKVKNPVRNLARHTDEFRHKLMLVDCKRLLNDKGSFGIILPVFEARY
                     FEEMAMDNGFIPKRKLFVSSKEGYDPYLVLAEYSLSDRACQSESLSIHPAGMNSYTQE
                     YRRLTEDFYLNF"
     gene            complement(34873..35403)
                     /locus_tag="PLF20_01035"
     CDS             complement(34873..35403)
                     /locus_tag="PLF20_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79626.1"
                     /translation="MIEQEDYIKTGQILRTYGISGALAISVELNYNPFEHARIDFMFV
                     AIDGSFVPFHILNSEPGPSGRWIIYLEDIPDPGKASFLCKRSYYIHKSYFPSIWPTRF
                     PAQRIEGFTIVDIHRGKLGEVQRLGGTPDNPLVIFDFQNMEVLLPINEHFIKNIDFNN
                     KSIKTDIPDDLLFLNQ"
     gene            complement(35482..36006)
                     /locus_tag="PLF20_01040"
     CDS             complement(35482..36006)
                     /locus_tag="PLF20_01040"
                     /inference="COORDINATES: protein motif:HMM:NF011094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOX79627.1"
                     /translation="MAVRMRLQRFGKKGQPFYHIVVADGRAPRDGRVIEKLGTYNPLP
                     NPAEIQIDMDRAIYWLNVGAQPSDTVKALLSYKGVLLKRHLLRGVEKGALTAEQAEAK
                     FQEWSAEKEAKIKAKITSKEAFQRDHLKSKLEAERKIAEERARQIAKKRSTETLNEEL
                     SNSEEAEISPSTEE"
     gene            complement(36112..36921)
                     /gene="lpxA"
                     /locus_tag="PLF20_01045"
     CDS             complement(36112..36921)
                     /gene="lpxA"
                     /locus_tag="PLF20_01045"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOX79628.1"
                     /translation="MHQPLTSIHPEAKIHPSVVIEPFTCIHKNVEIDEGTWIGPNVSI
                     LEGARIGKHCRVFPGAVISAIPQDLKFNGEESLVKIGDNTTIREFVTVNRGTKASGET
                     VVGNDCLIMAYVHIAHDCHIHDHVILSNAVNLAGHIEIFDWAIVGGMSAVHQFVRIGA
                     HSMIGGGSLVRKDVPPYVKAAREPLIFTGVNSIGLSRRGFPLETIRLIEDIYRIIYLK
                     GLNVSHALQTIQEEIPFSPELQNILNFIQGSSRGLIKGPGSERLLQTDDDF"
     gene            complement(37010..>38365)
                     /locus_tag="PLF20_01050"
     CDS             complement(37010..>38365)
                     /locus_tag="PLF20_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOX79629.1"
                     /translation="LHTGEFVTLTLKPAPINYGYKFVRSDIEGYPQIEALVDYVNNTD
                     RSTNLQKDNIHISTVEHLLAAIYGSGIDNILIEMDGNEVPILDGSSAPWVDLIHKARL
                     INQKAERVYFELKHNIIHTKSDRKSEIIAIPSDIQRFSAMIQFNSKVLDTQFATLDRI
                     DDFAKEIAPCRTFVFLHELEQLIQHNLIKGGDINNAIVLVDRILSEEELKRLAIYFDR
                     PEVKVLEEGVLNNLELHFMNEPARHKLLDLLGDIALTGFRFKAHVLATRPGHVVNVEF
                     ARKMREAYLKEHRKTEHTPQYDPNKPPLLDINDIKKLLPHRPPFLLIDKILEMTDSHV
                     VGLKNVTMNESFFAGHFPDEPVMPGVIQIEAMAQTGGVLVMSTVPDPENYLTYFMKIN
                     EVKFRHKVVPGDTLIFSLELISPIRRGLCHMRGKAFVGDKVVMEAEMLAQISKKPTPS
                     N"
BASE COUNT        11779 a         7557 c         7441 g        11588 t
ORIGIN      
        1 ttttaaagaa ggaaaagcta atgtataact aactgaataa gtggtatata aaaaatgttt
       61 aggaactttt agaattttgt cggttaatag tgattcataa tatatttata attgctcatt
      121 ataagccact tttaaaaaaa gaattatgag atttatagag aaaatttaac aaatcatagc
      181 atctcgcttc taaaaaacaa aaaccatctc tcttgaagta gcagagataa catatttaat
      241 aaaaaatcca agaaagatat aaaaacctat ttagatggtt tctgtttcga ccaccttagc
      301 caataagtcg gcataaggaa atgtgtcaat gcctaaataa cattgaccgc ttttgagctg
      361 attttaggag atgggcgttg tggaatgaga atgggactta gggacttgga aacgaggaga
      421 ctgggagacg tggtaattag tggatggtga ttagtaagtt aaaagtaaaa agttaaaagt
      481 tcataaagtt taaaacgaaa aacggaagaa gggagaacgg agaatggaga aagggaggaa
      541 gtgagattgg gggacgagga gacgaggaga cggtgtaaca gagaatgaaa tttgtaattt
      601 ggtaagttgt tgattgtctg ttgttagttg ttcgttgtca gttgttcgtt tgagtttgtt
      661 tgtaatttgg aatttggaat tttgtagttt ttagttgtta aaagagggtg gatcagagag
      721 aaaaataaaa atgttctcgc agatatcgca gaaaaaagcc ctcgcagata tcgcagaaaa
      781 tcagcattaa tcaaagagag aaagtatcac ttactatcat ctctaacaag ctatagacgt
      841 atagctaata aatagcgatg catctcaaat ttataagaga aaaataaaat agtatataca
      901 gtaattaata tacctcaatt tgacaaaagc atgtcaaatt ttaatctttt agcaaggaat
      961 gtaatttttg aagtaaatcg ggcaattgtt tcaaggcagc aagttctttc cattttatgc
     1021 gagcttctat agcaggcgca ccaaaataag tctttcctcc ttcgagtgat ttcgaaacac
     1081 tgctggtggc taatagaaca gctcctttcc caatgaccaa gtctttattt agcgatactc
     1141 tggcccaaag aaccacatta tcttcgaggg ttgtaacgcc agcaatggct gaatgggcac
     1201 caatcagaca atttttccca atgcgtgtat catgcccaat ttgtacatgg ttatccattt
     1261 ttgttccgtc gccaatatag gtttcggccg atactccttt atctatggca cataaagcac
     1321 ctatttcgac atcattgccc acgactactt ttccgcatga ttccaatttg cggtaacttc
     1381 cttcgtgatt ctggaaataa tacgcatcag cgcccaatac gctaccggag tgaatgataa
     1441 cccggtcgcc aatgatggta tggtcgtaaa tgctcacatt agcatgaatt atacaatttt
     1501 ttccgattac aacattattc ccaataaaaa ctccgggttg aacgatagtt ccttcaccaa
     1561 ttatagccga aggtgaaatc atagaagagg agggttgaaa gggatggaaa cgccttacca
     1621 gtgaaatata agcctcgaaa ggatcgggat gaatcaacag agccttgccg gcagggcatt
     1681 cgactttttg atcgataaga ataacactgg cctggctatt gaatgccttc tgatagtact
     1741 tggagtgatc gacaaaggtg aggtcgccgg gttctgcctt gtggatttca tttaatccct
     1801 taactaaagt ttggggatcg ccttccaatt tagcattcaa taaattggcc aacatgtgaa
     1861 gtggaataag ttgtggaaaa tccatgaatt taaaggaggt ataaggtaat aaaataggag
     1921 gaattcaaaa tcagtctttt actctttcca cataatctcc tatccgagta tcaatacgga
     1981 taatttcacc cgtattgata aaaagaggaa ctttaacgat agcaccggtt tccacggtag
     2041 ccggttttaa ggcgttagta gctgtatttc ctctttctcc cggttcagtg taagttacct
     2101 ccagctcaac atgcgtagga agctcgcacg aaataggctg ctcgttatcg gctaaaaaca
     2161 gaatttcgca tatcattcct tccttcaaaa attgaggagc atctattaaa tcttttggta
     2221 atgaaatctg ctcgaaatcc tcattatgca taaaataata atcttctcca tcggcataaa
     2281 gaaattgata ttcgcgtcgt tcaatcctga gaatgtcgat ttttacacca gccgtaaaag
     2341 tattgggaat cacccttcca gtgcgcaaat ttttcagttt tgttcgaaca aaagctccgc
     2401 ccttgccagg ttttacgtgc tgaaactcaa caatcgtata aggttctcca ttaaactcga
     2461 tacagagtcc attcctgaaa tctgcagttg ttgccataaa tcattggttt taattaaatt
     2521 ataatttgag tgcaaatgta cagctttttt aattgccgtc aatctttata ttgaacaaac
     2581 aaattggcca ataggatatt acgtaataaa aatagctctt gctgaaaatt ataaggtttc
     2641 attcgttgat gcgtcaattg tgatagcttc aattttttta ccattcgcat aatctgagtt
     2701 cgatatagac gttcggtgca taaggagagg atattcgaag agaaatagag gttccaatag
     2761 cgattggttg aggcgattaa attcttgaat ggcaccaatt atagagccat tgagcgagaa
     2821 acatgctcca atatattact taggataaga caacagcaga ttgaatgcaa taaatttgca
     2881 aaaaagaaag ggaattcaaa atggatatta ttagaagtcg ccgttccatt cgacggtata
     2941 ttcccgacga agtgaagaat gaagatatag aagggatgtt agaagccgca atgtatgcac
     3001 catcggcagg caaccagcga ccatgggagt tcattgtagt aagaaatgaa gccatgcgtg
     3061 aaataatgtc gaaaaccggg ccctattcat ttcatgctgc taaggctccc gtagttattc
     3121 ttgtatgtgg cgattcactt cacgaaacgc acaagaacta ttggcctgtg gattgttcgg
     3181 ctgctaccga aaatattcta ctcgaagcca cggcgcgcaa tttgggaagt gtttggttgg
     3241 gcgtttatcc aagacaagaa agaatggaat atttatccca tttatttcaa cttccagagc
     3301 atattcaccc ttttgctatg gtttgtattg gctatgctaa tgaaaaacct cctgcaagag
     3361 gattttatga tccatcaaag gtgtcgtggg aaaagtttgg caataagaag gtctaacttt
     3421 ataaaagatg atgggttatt attttccatc gaaaattttg acccattcat atctttttcg
     3481 gtttttccag taccctgtca ggtaggcata attaactata agaggcacaa cagttgttgc
     3541 ttcggcatac accatctgtt cgtaaaccgt atctactttt ccccacgaag cagcttcctt
     3601 gagagtcgaa ctcgaacaag cgccatctct aacgtcagca acagtaatct ggacagcata
     3661 cttgtgcatg ggtagattga atcctaaaac ttcgccacac actactgtat cttgggcaaa
     3721 atttttgggc gttccacctc caatcataaa taggcctgtt tggtcggctt tgatcttgat
     3781 ttgggtcagt tctaaaaaat cctttacaga gtcgatggaa acatgcctat cgggacgatt
     3841 ccattgatgt tttaccaaac caaaacccgc actgctatcg ctaaaagcag ggcaaaatat
     3901 ggggacattg ttttcgcaag ctatttgaac caatgaacca ggttttttgg ctttctgggt
     3961 taaatacctg cccatttcat aaataaattc tctcgaagaa taaggccttg gttcgaggtt
     4021 gtcggctatt tgttgtatgg tatcatcgca tgcttgtaat tcatcttcat cgataaaagt
     4081 atcataaatc cgatcgatat aatgattgcg gagttccttg tcgtcgacat aaggagaacc
     4141 tttataatgc ttgaagccaa gcgcttcgaa aaaatccatg tcaactatgc ttgcaccagt
     4201 agcaactaca gcatcaacca tattatattc aatcatatcg gcatatacct gcatgcaacc
     4261 tcccgcactg gtactccccg caagggtgag aataatggaa caatcttcat cgcttagcat
     4321 gaggttaaaa atatcagcag cctgtgctgt atcgcgggaa gagaacgaca ttccacgcat
     4381 ggcatcaatg atgggtcggc catcgaaagc agtaatgtca acatgttgaa tgacatcctt
     4441 taaaaaattt ttcttgtcca ttttgttttg caattaatag aatataaagg aaatttccga
     4501 ggattgactg agagaaaaag atcctgcaaa gttaaaagtt aaaacaatgc gatctaaagt
     4561 attaaaatcc aagtattttt aacattgatt tatagctttg ttcttttgcg aataattttg
     4621 tgtagtattc gccattctcg tcgaggtcga tgatcacgtg tttgggagca ggaaggaggc
     4681 aatgttgaag tccaccataa ccggagagtg attcttgata agctcctatg ttgaaaaatc
     4741 caatgtattg ttctttatca ttaaattttg gcaaaaagac agcgttcgaa tgggcttcgg
     4801 cattataaaa atcttggcta tcgcaggtaa gcccgcccag atgaaccctt tgatattcaa
     4861 cgtcccaatt attgactgcc aaaagaatgt aacgctgact gatcgcccag gtatcgggca
     4921 aagtcgtcat aaaagaacca tctatcatat tccagagttc gcgatcgttt tgttgctttt
     4981 gattgacaat agaaaacaat atggctccgc tttcccctac ggtataagtt ccaaactcgc
     5041 tgaatatatc cggctctggc actccatgct gcatacaggt attctttatc tgagaaataa
     5101 tttcttcagc catgtattca taatcgtaat taaactccaa cgaattttta ataggaaaac
     5161 ctccaccaat attcaaggca ttcaattcag gacaaatctt ttttaattcg cagtacaaat
     5221 tcacggcttt caaaagctca ttccaataat gagccgtatc cttgattcca gtgttgatga
     5281 aaaaatgcag catcttcaat tcaaacaaag gattgttctg gattttctcc tgataatacg
     5341 gaataatttg attatagcgt atccctaagc gagaggtgta aaactgaaac tgcggctctt
     5401 cttccgtagc aattcgaatc ccaatcttcg tttttcgttt caatcctgct tcgatatact
     5461 tatcaagctc gaacttatta tccagaacag gaatagtatt ctcgaaacct tcgtggatga
     5521 gatttacgat gttttcgata tattgtggtc gtttgaatcc attgcagatt atgtaattcg
     5581 acttctttaa atgaccagta tcatataatt cttcgataat attgatatca aaagccgatg
     5641 atgtttcgat atgaacatca ttttccagaa cttcttccag aataaaggag aaataagaac
     5701 tctttgtaca atagcaataa ttatattcac cattgtattc agttttagcc atagccacat
     5761 tgaaattgat tctggctttc tggatctgtt ccgaaattcg tggaagataa gttaatctta
     5821 caggtgttcc atactgttta atgatatcca ttaatggaat gttattccag taaagttcat
     5881 tatctatcac attgaactct tctttaggga actcaaaagt ttgctcgata aggtcaatat
     5941 atctgttttt catctttaaa gtaagtgaat taaattagac aaaatgattc aatttagcta
     6001 attcagattt ttaaatgagg cgcaaaaata caacttatat cttcatcttt tacgaaaaac
     6061 gaaaattttt tataaaagat atcacaatga tccataatac tttaattgaa tgattgtggt
     6121 ttgataaaaa gtatactggc caaattttct tatagaaaac tttcctaaat agcaagatgt
     6181 agttcactta catcttgaaa aatctaagca agtaagtaat ttggattaac gaaatgcagc
     6241 cattaataat tcgatattct taaaaggaag tgtaaaatat tcgccaatgg ttttgttggt
     6301 taaaatgcca ttgaaaatat aaactccttg tcgtaaacct ggatctcttg ttaaatactt
     6361 ttcgatgcca cctgtgtaac caatcttcag aatgagagga gaaaacacat tgcttattgc
     6421 ataagaagcc gtatgaggca ctttcgaggc aatattgggt acgcagtaat gggttacacc
     6481 attgacctga tatatgggat gtgaatgcga agtaggccgg gaagtttcca cacaacctcc
     6541 ctggtctatg ctaacatcaa caataacaga accctttttc atttgattta ccatttcttc
     6601 ggtaatgata acaggagttc tcccttcacg gctgtacaag gcagcaatga ctacatctgc
     6661 tgtttgaagg ctttttaaaa gaactttggg ttgaataata gaagtaaaaa ttctcgaccc
     6721 cagttgatat tgaatatttc gcagtttata aatcttatca tcgaagattt ttaccagtgc
     6781 acccatgccc aaagccgtgc gtgcagcata ttcggctacg gtgccaccgc ccaggataat
     6841 gacttcggaa ggattgatcc ctggaaaacc tcccaacata gaacctttac caaaaaccgg
     6901 atggctaaga tactccgcag caatcagcac agaggtcgtc ccagctattt cgctcatcga
     6961 acgcataacc ggaaaggcat tggaatcatc cttcacatat tcgaacccga aggcagtaat
     7021 ttttttctgc aacagcgaaa taaaataatt cttttgctgg ataaaggttt gtacagaaga
     7081 aaacaatacc tgacggggct tgagaaattc tatttcctgg tttttgggag gagccacctt
     7141 gacgatgatc tcagcctgat aaacttcctc ggtggtttcg caaattttag cacctgcttc
     7201 tgaaaattcc acatcagtaa attttgcatt attgcctgct cctctttcca gataaaccgt
     7261 atgcccattt tgtacgagtt cctgaacggc tgaaggtaca agggcaattc gattttcgtc
     7321 gggacttatt tcacgaggaa taccaatgct aaaacgtttt atggttcgag ctgctggttt
     7381 tagttgttcc tggggagcca gagtctcggc caaattcacc tggctcaaat cgttatggat
     7441 ggtttccatg ttcatattat ctacctgctg gtcgttttat tcgttatgtg gtggttcaag
     7501 aagctgaatt caagtcacaa atgcaacttt ttagttatac aaatttcgta ataaaaattc
     7561 atttagtgtt aagaaattca atttttttct tgatgtgtta ttcaaaatat cttctacccg
     7621 ctttgtattt tgatcagaga ggttttcaaa agaggttttt aaaaaaatac tgacgaataa
     7681 gtccgtctgt attttcatta gcccctcctt cccatgaatg atagggttta gcaaaaaaga
     7741 aatccacttc caattctgag ctaatcattt tatgctcgac aaactctttt ccgttatcgg
     7801 cggtgatcgt atgaatcttt cccttactgg gagacaatcg ttctatgaca ttttgagcaa
     7861 gctctttggc atccttgcta ttgagctttt caattgcgac ataactaatt actctgtcgt
     7921 tgatcgtcaa caacgctccc ttgtgatttt gacctatcat cgtattgatt tccaagtcgc
     7981 ccaatctttc ttttctttcg accatctctg gcctttgact tatgtcgacc ctctctttaa
     8041 taattcctct gctgtctttg gcgtttccac gttttcgata tctgcgtccc ttatgcctaa
     8101 ggtgagtata aagatcacca ccttcttttt tattcttcta tacatattga taaattcgtt
     8161 catgactgac acattccctg ccttccaatg caattcttcc tgctatttgt tcaggactga
     8221 aatcttttac aagataccca tctacatatt gtttgacttc ttctgtaaaa cgtaaatttt
     8281 tgcatttatt cttctgcctt ctttcgtatt ttcgttgtgc taaatctgct ctgtattccc
     8341 tgtttcgatt atcgcaatta agcttgagct cacgtgatac aaccgatttg tctttcccga
     8401 tagccaacgc tatgtctttt tgtttccatt cttgtagtat cattgcccag atttcgtatc
     8461 tttgttctac ggttaaatgt ttcatctttg taacttttta aggggaagag accaaagaaa
     8521 agcatttatt ccatcaaggc aaaggggaaa tcttttttct cctttgcctt gaaaaataat
     8581 ttctctttat cttttatttc ccctgaaaaa attgcattaa ttacttgaat ttacaagata
     8641 aattcctttt ttgaatgact tatgaccaat ttatcatgca aagaattgaa atttaatatt
     8701 ttatctcatg ttgtagatat aaacttatag aaaaaatatt tctcaattct aataatatct
     8761 cccaattaca aatgatatac ttatttatca aatgcaagta aatatcatat tttgatggtt
     8821 gtttatcaat tagttataaa tattaggaaa aattttacaa tgatccgact caaaatattt
     8881 aaccttattg cttgtttttt aaccgcacta tgaagggttg gtaacaaatc aaggcgataa
     8941 ggttaattaa gaattcaaaa caaagatttt tcgatcgaag ctttgcgtca aatgatatgg
     9001 cgaagatatt ttatgcttcc aaagtattga aattattctg taatcttttt ttgcagcact
     9061 tttttccctt gctgaatctt caaataataa accggttgcg gaagaagtgt gatgattcga
     9121 tcataatttc ctgagaattt tttcaagatt tcatgatgaa gtatgctgcc ttgttcgtcg
     9181 aaaaccgata cttctatatc tcctgattgc tctgtggtaa agcggagacg tattttaccg
     9241 cgaaccgatt tatttacgct gagtttgtcg aggtctaagg gttctttgac ctgggagggt
     9301 ttcaacgccg aatgggagag tttgttcaga tcatcggagt tcaaatcaac catatacagg
     9361 gttctggatt tcggtttcct ttctgaatat ctccagcttt gcggagtttg gcggaaggta
     9421 cgtaaatcgg ctctttgccg ggagcgattc cacacaatac tatcaattgt ctttctacca
     9481 tccttgaaaa taataagagt atctactcga ccattaggag tagacatgcg aatacattcg
     9541 atttccttgg tcagggtatc gcgtcctaca atcatctttc taataatttt aattgatttt
     9601 ccggcagtat ccataactaa ggaatcatct ctaaaagttc cgtaaggaca tccttggggc
     9661 aagtggtgaa aaccataaga atgaaaatca gacaaatgat gttctctaaa cttccaaccc
     9721 ttaaggtcag gacgagggaa ttcaggcatt tgacgaatgg cttcgtctaa ttgacgcatt
     9781 gccatttcga gatgtcgctt aacttccatg agattttcag ggataggggg agcttctctt
     9841 tgcataaaat ccctgaaatc agaaagatat tcttccatcg gttcatcatt ccattcttcg
     9901 gctgggaaaa gcatgtacaa attctccaat tcgttcatga tttcttccca gtttgcctta
     9961 tctggcaatg actgaaattc gggtgaaaga ttctgatttt tagatggtgg agaaaatggt
    10021 ggctgattct gtccaaatgc cagcgaacta aagcctaaaa ttaagcttaa tacgaaagga
    10081 atcatcaggc aatttaaaat acgaactaaa gatttcatga catttttgtt ttaattattg
    10141 aacgaacaaa attaccaagc tgttcataat cattatgtta atatgccgtt aaagtttgtt
    10201 aaagaaaagt taaatagcca aataggcggg caaatcaatt taattttgca ttatgaataa
    10261 atcagttata cgggttgtta ttatattgag tggcatttcg ctggtaagct tggtgatact
    10321 gcagtttgta tggttcaaga atatgctgca aatgcgtgag aacgagttcg atagagatgt
    10381 aatggcagcc gttgccgaaa ctgcacggcg gctcgaacgc gatcagacca tgttttttgt
    10441 ttcgaacaga ctcagaatgc aagctttaac cgatacgatc cttgcctcaa ctcatccgaa
    10501 aaatcgtacg gccacgatca ataaaaccag tacaaacatg aattcaattc tgcccgatag
    10561 tcttcctcct cgtcgaaggg ttaacctaaa acatcgtcaa tgggtggtgc agcaaaatgc
    10621 cagaggtgaa ataattgttg aaactgagaa ccaattggat acattaactt cgatggatat
    10681 gtcggtttta ttcgatcccc gtttcgaacg tatgctacct ttcgccaatc tctctgcaga
    10741 tgaatattat aatgcgatca tcagacgtca ggaaagattt tttagagaac aaagacagat
    10801 gatggataaa ttcgattcag tatttatgcg ttatcaaggg agaataattt ccgatcgtcc
    10861 caaaccacgg cctcagaagc ttaatccaaa tcccgcattt cagaagcaag tcgaagatct
    10921 tcaatcgcaa accactctac ttagcgatgc gcttgatcgt ctgattttcg aaataaaaca
    10981 actcgaaatg cctgctcgtg aacggcttga tgtgccagct tcgcatgaaa ttttgaaaca
    11041 agaattgaaa aaacacaata tcgatctacc ttttgaatat gccatctttg atgaggaaag
    11101 ggataccctc gtccgaagcc ctcgcttcaa ccctttatcc agcactaaag tctatgaggc
    11161 taatctcttt actgatcgtt ttttccaatc ggccgatcat atcgctttgt attttcccga
    11221 tcgcagcacc catttgctgc gttctatgct ttggattatc acaggatcgg cgctttttac
    11281 cctgataata gttattacct ttatcataag tattctggtg attctgcgtc agaaaaaaat
    11341 ttccgatgtt aaatcagatt ttattaataa tatgacgcat gaatttaaaa ctccaattgc
    11401 tacgatttct ctggccgcag atagcctcgt taacgcacgt attatcaatg atcccgaaaa
    11461 gattcattat ttcacccgcg taattaagga agaaagcaaa agaatgaaca cacaggttga
    11521 aagcatgctg caaatggcac tcctggagaa aaaagacttt tcacttcatc tgcaacctat
    11581 ggatgcacac gacttgatgg ctcaggctgt tcaaaatatc tccatccaga ttcacaaacg
    11641 aggaggtgag atccataccg atttgcaggc tacaaatccg atcgtcgaag ttgatgaagt
    11701 tcatttcctg aatgtattat tcaacctttt ggataatgcc aacaaatatt ctccacaatc
    11761 tcctcaaata gaggtaaata catggaatgg ccatcattct ttattcattt cggttaaaga
    11821 tcatggaatg ggtatggata gagaaacccg aaatcgtgcc ttcgaaaaat tttatcgaaa
    11881 atctactgga aacattcata acgtcaaagg atttggattg ggtttgagtt atgtaaaagc
    11941 catcataatg gcctccggtg gaaacataca tatacaaagt gaacctggaa acggtagcga
    12001 atttattatc gaactgcctc aaataaacaa tgacgatgaa tcaacaatcg attaaaatat
    12061 ttctggttga agacgacctt aactttggag ctatccttaa atcatacctc gagctcaatg
    12121 attacgaagt aatatgggtg aacgatggca aggatgccat gacagctttt tatcaaggga
    12181 aattcgacat ttgtattctc gacgtgatgc tacccaatat tgacggtttt agcatcgcaa
    12241 aggaaatcaa acattttcgc cccgacatcc ccctcatctt tcttacagca aaaacactta
    12301 aaaatgatgt tttagaaggg tttaaactgg gcgccgacga ttatattaca aaacccttcg
    12361 atagcgatgt tttactttat aagataagag ccattctgaa aaggcacggt caagtttctc
    12421 aaaaggaaga acagcgagaa ttccagatcg gcgaatataa atttgatgca tttctgcgaa
    12481 tcattaccca taatgacgaa agccgcaaga tcagtccaaa ggaagctgaa ctcctcaaaa
    12541 tgctatgtat gaatatgaac gatatcgttc ctcgtgaact tgcattgaaa acaatttggg
    12601 gagatgacaa ttatttcaca actcgaagca tggatgtgtt tattaccaaa ttacgcaagt
    12661 atctgagcaa agaccccaat gtagagatag aaaatatcca cggaacagga tatagacttt
    12721 tactgaaaaa ctcttagagc ccgatatgtt cagaactttt catgataaac cttcgaaatt
    12781 ccccaaaaga aaattcgaat ctattttaat gaatttatcc ctttgtactc ggtgagaaat
    12841 tgaagaacat tcttttctat tctttcttct atattatcga gagaagtcct gacaaatttt
    12901 tctcctgtaa ttttttcata aagttcgata tacctttccg acacgctatt tacaaaatcg
    12961 tcatccatat caggaactcg ctgtccgatc tgacccatga accctctttc cataagccat
    13021 tctcttacga attctttcga aagctgcaac tgtggaagac ctttagcgaa acgttcttgg
    13081 taaccttcgg cataaaaata tcgcgatgaa tcgggagtat gaacctcgtc gatcaagtag
    13141 attttgccat ccctattgcc aaactcatac tttgtatcca ccagaataag tcctttttgt
    13201 gcagccatta tcgaacctcg ttcgaaaagg cggcgagtat attcttctat ctgtagataa
    13261 tcttcttcgg gaattaatcc attttggatg atttcattgc gagtgatgtc catatcgtgt
    13321 ccttcagtgg ctttagtagt gggagttatg ataggctgtg gaaatggttg atgttctttc
    13381 atgccttcgg gcagaggaac tccgcaaatt tgtcgggctc cttttagata agctctccat
    13441 gaacttcctg tcaaataagc ccggatgatc atttctacag gatacggttt gcataagtaa
    13501 cctgtagtaa ccatgggatc gggggtggca atcttccaat tgggaacgat gtcggacgtg
    13561 gcatccagaa agtaggcggc gatttggttc aaaacctttc ccttgtaagg tatggctctt
    13621 ggcaaaacaa cgtcgaaggc cgaaatccta tcggttgcta tcatcaccag tagatcatcc
    13681 acaaaataga catcgcgcac cttgccataa tatatttttg tttgtccggg gaagtggtaa
    13741 ttcgtttcaa gaactgcgtt atacatattt tttaaaaaaa gtataatgat gaattaaagt
    13801 ggggtttcgt ttttattgtt tttttaatgt tgagggttat cacggccgta agcttctata
    13861 acatctttta ccagacggtg tcggataata tcgcgttggt caagaaagac gaagtcaatt
    13921 ccctgaatgc ctttgaggat ttgagtggca tgaacaaggc ccgatttttg ggatggagga
    13981 aggtcgatct gagtgatatc gccagtaatg ataaatttgg cactgcgtcc catacgcgtg
    14041 aggaacattt tcaattggtt aagagtggca ttttgtgctt catcgaggat agcaaaggca
    14101 tggtcgaggg tacgccctct cataaaggcc aggggggcaa cttcgatggt tccatcttcg
    14161 agataggtga gcaatttctg cgtggggagc atatcgcgca gtgcatcgta aagtggctga
    14221 agataaggat cgagtttatc ttttaaatca ccggggagga agccaagatt ttcaccagct
    14281 tcgacggcag gacgtgtcag aacgattcgc tttacttccc tattttttaa agctttaacg
    14341 gcaagtgcaa ccgcagtata ggtttttccc gtgcctgccg gcccaatggc aaataccatg
    14401 tcgttcttct ggctgctttc gaccagcaat cgctgattga tagtacgcgc ccttacctgt
    14461 aacccatgat ttccataaac caaaatatca ttcccttcta tcggcctaac ctgattaaac
    14521 tcttcttctc cttcgttgct aaaaatctcg aaaagatcat tttcggtgat tttgccaaat
    14581 tcatcgtaat gtaccaataa gagtccaaaa agtcgatcga aaagatcaat ttgcgatttc
    14641 tctccaataa tcttgattac atcgcctctg gctataattt tcaattgagg aaaacgatct
    14701 cttattaggt ttagatactg gtcgttagga ccatatattt cgagcgggct ataagattcg
    14761 atggaaatga ttctttcgtt cattcagctt gattgggcta ctcttaaaat ataaaactgt
    14821 gatttttgta attttgggat ttgcaaaatt acagtaaaaa accacgcatt caccataatt
    14881 ttaaatgtca attataacac ttacaagcga ctggggacta aaggatcatt atctatcagt
    14941 ggtaaaggcg gcaatcttaa gtcgcctgcc cgagacgata attattgaca tcagtcatca
    15001 aatcccgcca ttcgatttaa atcaggcttc gtttatcata agaaattgtt ggaagggttt
    15061 tcccaaaggt acagtgcata ttattggaat taacaccgag gctgggatta aatcgccaca
    15121 taccgcagtg gaaatggaag ggcattattt tatcggtgcg gataatggga ttttcagcct
    15181 gattttcgac aagcagcccg aacgcattat cgagatcgaa atagcgcagg atagtgaata
    15241 tttcactttt tcgacgaggg atgtctttgt aaaagccgca gtgcatctat taaaaggcaa
    15301 ttctattgaa gacctgggta tacctcgaaa tagcctaaca ccatgtgctt ccttccaacc
    15361 tgttattact caaaatgcta ttacgggtaa ggttatttat gtagatgttt atgaaaatgt
    15421 tttcactaac ataagtcgtg aaatgtttca gcaagtgata aaaggaagac atttccagat
    15481 cgaattgggg tatatcaaaa ataagatcaa ccggatttcc gattcttatg gggatgttcc
    15541 tgaaggggaa attgtagctc tattttcgag tacagggctt ttgcagattg ccattaatca
    15601 tggaaacgcc tcaggattgc ttggccttag cattgacgac agcatcagaa tcgttttcga
    15661 ataagtccgt cgtaaatgtt accgcttctg agttgcggga gaaatggtaa tagtaaattg
    15721 aaaggtaaaa gcgaaaaaaa taagataaaa aaatggaatg agatacaggg taccaataga
    15781 caagatgaac ggaaaattga gaggcaagaa tacaggaaaa tgaagcgaca ttttcatagt
    15841 aattcatcat tcgcaaattt cgggttatcc caaattatct atgaagaact gcaaaagaaa
    15901 tcgtttcgat ctatcctgac aaacagttgg caaatgggaa agaaaagtgg ccgagaaggg
    15961 cttctccctt ctcggccacc aaaaataaaa tttcatgttt catgctattt ccgaacactt
    16021 cttccctctt tgcaataatc tatctcgata aaaaaaccag gcaagaactg cattattcca
    16081 aaatccattc ttcacgtaaa tcccaattgg cccttatttc tattggtgcc ttaaagtatt
    16141 gtactttttc gggttgacgt ttttttctgt aatttcctgt atcccatttc ctatttcgat
    16201 tgcaatccaa gacagctttc aaaataaatt tttttggcag caacattcca tagtctattt
    16261 cacaaactaa atcattgata atatcctggc ggacaaggtt ttctttttca tcgagcaatt
    16321 gaataaggta ttgattattg ctggcaggag aaatcaactt caggtgaaaa ctcccataag
    16381 catcggcagc acgcgtagta aagcgtgcgg taatgctatc gttggccagt ccgaaaatat
    16441 ccacaaaagt cttggaagga ataatcaact gataagaaga attataatcg agagaatatt
    16501 tgaccaaagc cttgcgtttg aggctatcgg caaattcgaa tttaacccat aaggtatctt
    16561 tgcttgtgaa taaaagtaat gagtccgatt tcacagaatc aacaggatga ttgaacttca
    16621 aaataaaagg tttgaaggca tcggtttggc ctgataaggt ttctaccatc aaggattcgg
    16681 ttttggtctt tttacttctt gtacctatag tttcgcgttt caaaggaagc accaaagtat
    16741 cgggctgaat tcctgtggta taaatacata ttttgacact atcgaaagga caagatggaa
    16801 accagagctt tatggaatcc tgatttctat tgatttctaa taaaggttga tacaagctgt
    16861 cgaatggtgg caacagtcga taagaaaaat cttcgattga cctcttgaaa ttcaaaaaaa
    16921 tctgaccatt gcctgtgttt tcacgtcgaa gcaagcgctg aaccgtatct tcttcaacaa
    16981 aggaaaacaa ccgataagat acgaaggata aatggggctg tctaacaaca gtagaatcat
    17041 tcttagccag ggtatctggc agagaagaaa aatattcctt tgtttggctt acaactttcg
    17101 cgagggtatc ggaaggcgct gtatcatttg tatcacgtaa gtgttgaatt tgtcgaagcg
    17161 tatcctgaaa taacatgtgt tggattgagt ctttcagttc ctgatgagca attgtatcgt
    17221 gcagcaggct gtcaggcata gacacagcca attttggaac aaatgaagta tcaggggcag
    17281 gaggaatata gaatgccctt accaaagtgt cgctaaaagc aatttcttcc tctgcaaggt
    17341 caaaaaaata attgtaattc tgatcaatca atgcaaagag cagatatttt tcgtcgcgta
    17401 aataattgag gttgaacgaa ccatccttgc gagtgcgggc tgcataagaa ggtgggactt
    17461 tcaaaggaag ttgttcgagc ggaagggtat cgttattggc gtaatagagc ataacgacca
    17521 tattttccac gggtttcagg ttgaaagcat ttacaaccga acccgtcata gttaatgaat
    17581 cgatcactgc accggtcgaa aatacatatt gaaaatcggg cagaggatta ttctcatgaa
    17641 ggtcggtgat ggcatcggca aaatacaaag aataggtggt attgggcctt aaagtatcct
    17701 tcaagtcgat ctcgagtgat ttccccttta ccttgaactc tggaagattg agtgaaggag
    17761 gcgacatcag aaattttttg tttatttctt tcagaaccac atattcatcg aaggttaact
    17821 taatgtgaga tgctgtaaag tgagtgctta gattgggagg aacggcataa agcaactttg
    17881 gtggatccgt atcctttggc cctccctgtg gtaccacagg attagcacag tgacacatca
    17941 agagcaacag gccaagacca agcgccagca tggcattatc aatcgccttc tttagaattc
    18001 ttataaaata tcttcgggac tccatactta aagtttcgac tgaattacat caaaagtaaa
    18061 acctttttcg agcatggcct ccagatatga tggtaaaacc tctattaggc aaggaaatgc
    18121 cttttcgtta tcgtgaaaaa cgatgatatc gcccgatcgc gttcgtcgaa gcagggttcg
    18181 aagtatttct tccgatgaaa ggcttcgatt gtaatcaccg ctcagcaacg tccacaaaat
    18241 gatttggtat tgtcgtgaaa gcactctaac ctgtgaaacc ctcatccttc cgtatggcgg
    18301 acgaaataga ttcgtcggta ttagctccct gcaagcctcg atgtctttga aatactcctt
    18361 attggaggtc ttccatccat ttttatgact gaatgaatga ttccctatgt tatgcccatt
    18421 attttttatt tcctgaaaga cttcgggata agctcgaatg tttttcccta agcaaaaaaa
    18481 agtggccttt acttgaaagc gattgagaat atccaaaact ttcggggtaa tttgtgaatg
    18541 agggccatca tcgaaggtta gatagaggca ttgacctgtc cgaggcatac gagtaattac
    18601 aaatggagca aatatccatt cgacagccct gggaaaacac gtcgtcgctt ttatcataaa
    18661 catcaaggtt cgaagttttg ataaatctga gtaaaataac attttaaatt tttctcaaaa
    18721 gcctccttgc ttttatcgcc aatttcctcc tgcccaaatt tgcaggcagt ttcagcaata
    18781 taattcaata tgccaagatt caagttgatt tcgtattcgt agcgatgaag caaattgggc
    18841 aacaactcga aataatagcc gagctcggcc gaggattgat tcatcatcac ggtcattatt
    18901 tcgttgcctt tcgaaatctc acctgcccca taatacactt cggcaagcgc caaagaaaaa
    18961 acatcataag gaataacgga attcggaagg tttacgatac atcgatcgca tactctaacc
    19021 gccaattcct tttccccttc cttcaacaag gtatcggcca gacttctgaa tatattgcgt
    19081 agcaaagtgc aatttttcaa atgattttcc gaataatata tctcaggatt attcattttc
    19141 acctgcgaac gattcatcaa atgatcgtaa agaatctccg aatcaatccc tcccatacga
    19201 tcatgccttg atttcatcaa aataggaacc aggcgatagg ccatgccttc gagacgaaaa
    19261 tactcttcaa gtcctaaaaa ctcgcgggtg tttcccgtca aaccaaaata taccggcctt
    19321 ccccagttat tattcgctaa gatattcaaa acaattaagg tatttttttc gaattccctg
    19381 cccggtattt cgaaaaacat tcgatctacg attctatcct tctgatcaga gtcgataaca
    19441 ccatgattca acactgcagt cttgtcgata ttcaatacaa atcgattcga tggtaaataa
    19501 tctattgtat cggtttcgat gacatatttg aatttttgag gttccaccga catttgctcg
    19561 aatatactgc acagatttag cgtatcggtc agcgaatcat catccgaaat aaaataaata
    19621 tagtcgtgtg ttccttgctt ataaaaagaa aaatcaagtt gaatgggtag gggtgctgat
    19681 tcataaactt tgcgtttcat ctgttcgatg taccaatcga ggctcaaaag actcaagtta
    19741 cacactctga tatcggtacg gtaattttct acctcctgaa ggtaccaaag aggaaaggta
    19801 tcgttgtcgc caaaagtaaa caaaatggca ttggatgcgc agctatccag ataggcttta
    19861 gccatctgtg tagtggcata tcggttcgag cggttatggt cgttccaccc ctgggcggcc
    19921 aacacaccgg ttacacaaag aagattcaaa cctgtggtca gaagaatcga ctttctgcca
    19981 tttatccatt ttgtcatacc tgaggcaaac gcacccgtgc ccattcctat ccagatggca
    20041 aaggcgcaga acgaagccac ataggcataa tctctttcac ggggctgcgg actgtattga
    20101 tttagataaa cgatgatggc aattcccgtc ataatgaaaa acaccaaaac caccatcaag
    20161 cttttccaat cgttcttata atgatatacc ataccaacca gaccaaacaa caaaggcaaa
    20221 agataatacc gattccatgc cctgcttttt ttatgttcgg gcaggtgact caaagggcca
    20281 actctccatt tatccaagaa attccacccc gaaatccagt ttccattggc tttatcggag
    20341 aatccttgag aatcattctg gcgaccagaa aaattccata aaaaatatct ccagtacatt
    20401 tggttcagtt gataatttat cagaaagccc aaatgatctc caaaagtagg cctgtaattt
    20461 tcgtcatcgg gaataccagc ccaaatctta tattcctgaa tatgtttgta cgaacgagaa
    20521 tacattcttg gaaaaaacat tcgagaatta taaaccggtt tcgcagcctt cctactatct
    20581 ttcgcaacat aacgtccagc cgtgtaatct ttcatgtaaa ctggctcacc atccttataa
    20641 tcgacaatat ctgccgtgaa atcggctccg taaaccaaag gccaattgcc atattgctcc
    20701 cggttaaggt atgacaaaag ccctaaggca tccttaggat cattttcgtt gatgggagga
    20761 ttggcattag ccctgataat gatcattaaa aacgatgaat agccaataat tataaagctc
    20821 aacgaaagca gaatcgtatt catcacctgc ctgtgcttca tcaggtacaa aataccccat
    20881 cccaaaccta aaacaatgaa acctcgtatt ataaattcta ttaaggtttt agagcccgtc
    20941 atgacaatga ttagaaccaa taaactcacc attaggaaca aaccacggaa aaaatgattg
    21001 ggataatgtg taaaaagaat tccagaaatc agcatagtta ctataagtga agccgaaaag
    21061 aaagaaccac ttccgaaagg cagccctaat cgatttacca taaataactc ggtccgggca
    21121 aataagccag gtatttcagg aataagacca taaagaataa aggttaacaa aaccatcgaa
    21181 attgccaatg ctccgatgac accggccaca gttggccttc ttacgtgaaa ataaatcaat
    21241 aatatgatag cgggaatggt caaaagattt aattgatgga ctccaatgga caaaccaatg
    21301 agataagcta tcaatatcag ccagcgaatg ccatgagaat cagaagatcc tctttcccac
    21361 cgcaagatag cccaaaacat aatggcagta aaaaatgaag ataaggcata aacctcggct
    21421 tcaacggccg aaaaccagaa tgaatcggta aacagatagg ccaaagcccc gctaagacca
    21481 attcccaaaa tcagatactc attttcggct ctcacttcat cattcttcga aagaaaatgc
    21541 gaaaccagta aagtaatact ccagtataga aacattacag tgaaagcact ggctatggca
    21601 gacatgaggt tgatactgga agctaccttc gaaatgtctc caaaagaaag caaagaaaat
    21661 aatcgcccta aaagttgata caatggagct cccggcggat ggcctatttg caacttataa
    21721 gccgtactga tatattcacc acaatcccaa taacttgctg ttggttcgac ggtagaaata
    21781 taaacagcgg cagctataaa aaaaatctcc cagccgagga ttttatcgat tttctgaaac
    21841 gaaaagctca tgagaagccg agaggagaaa attaatatcg gcgaaattac tgaaaaagtt
    21901 aagatgcaac tgcaaaggcg aaaaataata actttgcaac gagtgtaata accgagaaaa
    21961 tatttttaga aatgctcaaa ctggtattgg ttcgtcatgg tgaaagtgtg tggaacaagg
    22021 aaaatcgttt cacaggttgg acagatgtcg atttatcgga aaaaggtgta gaagaagccc
    22081 atcaggcagg taaggtatta aaagaaaatg gttttgactt taaggtatcc tatacttcat
    22141 atctgaaacg cgccataaaa acgctgtgga ttattctgga agaaatggac ttgatgtgga
    22201 ttccggaata taaaacatgg agactcaacg agaagcatta tggtaaatta caaggcctaa
    22261 ataaaataga aactgccgaa aaatatggag aagaacaggt caagatatgg aggcgttcgt
    22321 atgatattcc tcccgaaccc ctcagccccg atgatccacg caatccccgg ctcgatccgc
    22381 gctatgcaga aataggtgat gatgcaccac tgaccgaagc cctgaaagat accattgccc
    22441 gtattgtccc ttactgggaa aacgaaatga gtataaaact ccgtcaatat aaggaagtac
    22501 ttgtggcagc acacggcaat agtctccgcg gaatcgtcaa atacctgaaa aatatgaacg
    22561 aaaaggaaat catcgacctg aatctgccta ctggcattcc ttacatcttc gaattcgatg
    22621 atgatatgaa tctgcaaaag gattatttcc ttggcgatga agaagaaatt cgtaaagcaa
    22681 tggaagctgt tgccaatcaa gcgaagaaaa aataataccc tttatattta tatcggatta
    22741 cattgaatgc attatggttg ttatcaatca aaagagtata aattttattt caaaaattca
    22801 tcaatatttg cagataaatg gatagacgaa atcatgagcc ttgattgggc cgagtaaaat
    22861 ttttgaaaaa ttatgcctca acagtgggtt atacccgata cgcatggtta tctaaaaacg
    22921 cttcgagcat tattagaaga gcagattcgt ccttcgaaat ttgacgagat ttattttttg
    22981 ggcgatttta tcgacagagg ccccgattcg aaaggtttga tcgattactt gattcattta
    23041 aaggacgatg gttatcgact acgaatgttg aagggcaacc atgaagaatg gtgcattaga
    23101 gcatacgaag aggaattgca gctaaagaag agttttttgg gtttgaaaaa accaaatatt
    23161 caaaaagccg aatggatgag gtttggaggc aaagaaactc ttaaaagctt caaggtaacc
    23221 aacttgaggt acttcccttc tgaatacata gaatggatgc accaactgga atattacata
    23281 gagctcgaag actattatct tgtgcatgca gggtttaatt tcgaaacaga ttctattttc
    23341 gacgatcgcg acgctatgct ctggattaga gatttcaatg tttctcccga aaaaataaat
    23401 cacaaaatca ttattcatgg gcatgtgcct gtaagccatg agtttatcga tttcaccatt
    23461 cgtaagaaac aatatcactt tatcccatta gacaatggag tttatataaa gaaaaaaggt
    23521 tttggcaatc tcacggcttt cgaaatcaag caacaaattc ttcttgttca accaagcctc
    23581 gacgattgat cggcttccat ccttctattt caggctcgtt acactgccat taatttgcct
    23641 cattaaacca ttccttattc tccaaacccc attccctaac actaatgtta ggatctcttg
    23701 aaaatacgtt tccacgccga ttctttttca acttccctgt cgtcggaata atatccatag
    23761 ccataaccat ggccatatcc atatccatag ccatagccat agccatagcc gtaaccatag
    23821 ccataaatat tcttagccat tttgatatca tttacaagaa cagaaaaatt aggaagtttg
    23881 cggttttcga tatccttaat aatagccccg aagatctttt tattggttac gccttggcgg
    23941 atgataaaaa tattctggtc ggaatgtttc atgaggagga aggcatcggt aaccacacct
    24001 acgggaggag tatcgaggat aatataatcg taatgttttt ttagataatc gaatagatcg
    24061 tcggttttgg gcgaagccag taattcggcc gggttaggag gaactggacc tcccaatatt
    24121 acatcgagat tttcgatttt cgtaggatgg ataatttctt caattttgtg tttaccaatg
    24181 atgaatgaag taattccatc ggtattagcc agtccaaagt cctgatgaag cttaggtttg
    24241 cgcaggtcgg ctcccattaa caatgttttt ttatcattga tagcgaaaac ggtggcaaga
    24301 ttgatggcta taaatgtttt tccggctcca acaatatcgg aggtaatcag gatagtttgt
    24361 ttttcttttc cctgtgcccg aaagataata ttggtcctca aggcacggaa agcttcggca
    24421 atgcttgatt taggccattc ggccacaacc atcttagctt ctcttgtgtt atgagaaata
    24481 tacccgataa taggcaagct ggtaattttt tcaatatctt ttttctcgat aatcttatcg
    24541 ttgaaataat ctttgatgaa aacataaccg ccaggaataa cgagacctaa tgtaattgca
    24601 atcagataat tgaggctttt cttggggaat atgggtgatt taccttcagc acgagctaca
    24661 tcgattacct ggttatcagg caggttggaa gcctttgtaa tctgagcctc ggctcgtttt
    24721 tgcaaaagaa aagtaaataa ggtattttgc aggttgaact cccgttcgaa attgagtaaa
    24781 cgtcgttggc tggtgggcaa ctggctgatt tgctgattca aatcttgaat acgtttttcg
    24841 atatcagcca gggcaatatt cgaagtgttg acgatgttgt tgatattttc taccagatta
    24901 gccttggcat tggcaatttt actttcaatt tgtatgatag ccggatttcg tggagtagag
    24961 cttaacaaca gacccgaacg ttcttcataa aggctggtaa ggtttttgat gagttcggcc
    25021 agcacgggat catctatacc cattgccgat ggtacgataa tatcgttcat ctcgttcttc
    25081 gactgcacgt aatctttcaa ataattatag tatttgtttt tcaccaacag gtctgctttc
    25141 tgcttgtcga gttctttcat gtattggaat acctgctgag attcgaaatc gagttccatg
    25201 atcttggctt cctttttgaa tccttcgagc tgagattcgg caatcgaaag actatcacga
    25261 atgccgatga gttcacgatc gataaattct atggtttttg tggcgatgat gtttttttga
    25321 tccaggtcgc gctcaagata tttactggtg agtgtattga gaaaatctac ggttttttct
    25381 acttgactgc ctttggcctt aatttcgagg atagatgctt cacgattaat cggttctatc
    25441 tttatattgt gaaattcttt taccagactg ggcaggtcgt taaaagtaaa gaagaacgtt
    25501 ttattgacat gttctttcga attgaagttg tagttgagta aaactctgaa tttgtaataa
    25561 gggcccgaaa tggtagttcc aaaatcataa atctggttca gtttgatggt ttttatttta
    25621 gtctctccgt cttgttgttc ctcaataaat ttattattct cgaaatcgaa gagtttgatg
    25681 ttttcaccct gggtttcgag tttaaattgt ctattattaa gtatagtaac ataaaatctc
    25741 agatgtacgg gttgtggatg gatagaatca tagaatactg taaaaggaga ttttctatag
    25801 agttctctta cgatgaagtt gttttcggaa taataggtta catcaacatt taatactttc
    25861 acggcttcgg atgtaagtgt gtagctactg agaattccca gttcattttg aatattttgc
    25921 tgggaattca taaaaccgag gcccatcagt gcttgggcat ttatttcctt attgccctta
    25981 tcctgaatca atactgtggt agaaacctca taggtggggg tggtgtattt gttgaacaaa
    26041 aaggcaatca ccaaagcaac aaatatggcg agtgcgaaca gataccagta tctcaagaac
    26101 ttgaaaaaaa tctgtttgaa atcgatattt tcttcttgtt gctgataatc gggcatattg
    26161 acgttgttat ccacttttcg atacttttaa gattctattt gaaataattg agaatgagca
    26221 atgttgttgt gatagtagaa aaaagaaggt cgtacgggaa ggcagcaaaa gcgtattgtt
    26281 tagctcttaa aggctcaaca tagacaatgt cattaggaag taaataaagg taaggcgatt
    26341 cgagaaaatt ctttttagtc aaatcaatac gatgcaattt ggcaccgttt tggtcttgcc
    26401 ttaccagcac cactttattt cggtcgctaa atacggtaag atcgccagct ttagccaatg
    26461 cctcataaat attgatttta tcctgataaa taagataagt cccgggattt tttacctctc
    26521 ccaatacggt aatcttgaaa ttgaccagtt tgacgattac agtggtttca accaggtatt
    26581 ggtcgacaat gtcctgaagt tttttcgaca cctcatctac agtgagcccc gagacaaaaa
    26641 ttttgcccac gaaaggaaaa tgaatgtagc ccgaatcgct aacggtatag cttgtaagat
    26701 atatgtttgc atcactataa cctatattgg tcgctctacc catgtcttca taattgaaga
    26761 acttataggt tttttcgtcc aggctttgaa ctttgatgta aaggttatcg cccaccttca
    26821 aacgataatc gggttttggg gaaaattcga aaatctcgag tgtatcttcc ttggcaagtg
    26881 attgaaaata tttgattttt ttctgaggta cacacgaaat catcatcatc gataatagaa
    26941 gaacctgaat aaaaatacct ccgtaagcgt tgaaactttt tttcattcct gagtgtttat
    27001 gcatttatga taagacatct tgtaagccat tgctgttgat cggacccgat aggagagttt
    27061 ttattgtaag gatttcaatt aacctgttca actatatggc cctttagtgt ctttttggat
    27121 gaccttattt tctggcgcaa aaataaatta tttttttcat gaaacaaccc ataccatgca
    27181 taattttctc cttcaatcga aaaatttcag attctttcaa cattcactca aaattcgaca
    27241 agccctatca tgcaaatagc agcaccatca atctcgtgta tgaagatttc aagagcacca
    27301 tagatttgcc atttctagtt ataggaagaa tcgaacatcc tacttaaggc atcgacacca
    27361 cgtaaagaac atgtatcttc gcaagcataa aaaacctata aaattccaca ggctatgaat
    27421 acaaagaaag atatgcttcc ccaaacttct gatactaaat cttcgaaaaa atccggatca
    27481 aagaaaatat tcgttctcga cacctcggtg attctataca atcatgcttc tttctattct
    27541 ttcgatgata acgacattgc catacccatc accgtattgg aagaactcga taatttcaag
    27601 aaagggaacg aaatcaagaa tttcgaagcc agagaattca tccgcatcat cgatagatta
    27661 tcgcataaga agaatcttac cgattgggtt gcaatcgaca atggcaaagg tggaagtttc
    27721 agagtaattt tggatcaacc tcacgaacaa aaggttcagc aaatttttgg tggatataaa
    27781 aatgaccatc gtattttaaa tgccgctctt acactaaaag aaatttatcc agagcgcaaa
    27841 gtcattttag tttcgaaaga catcaatttg agaataaaag ccaaagctct caatcttcag
    27901 gctgaagact tcgaaacggg gaaaatcaag gatattgaga atctcgatcc gggcaagaag
    27961 gtaatatccc atatagctcc gcaagttcta tcgaaactct ggactacggg gaagtgttct
    28021 ccctccgaaa ttggtgaaaa agagcctatc ccaaattatt attatatttt tcagaatgca
    28081 agtgagaacg aaaatccaac gctggcattt tataacccga aaagcggaaa catagagcgc
    28141 atcgatcctc agcctgtaga gcgcatcatg cctcgtaacc ccgaacaggc cattgcactt
    28201 catgccatta caaatcccaa tgtaaagttg gttactttga taggaatagc cggaacaggt
    28261 aaaactctgc tggctctggc aggtgccctc gaacaacgga ggctttacaa gcaaatttat
    28321 ctagcaaggc ctatcgtacc cctgagcaat aaggatattg gttttcttcc gggcagcgcc
    28381 gaagaaaaaa tcagtcctta catggaacct ttatacgaca atctaaaatt catcaaaaat
    28441 caatattccg aaaaggaaag agaatataaa cttttcgatg agatgctcga aaacggaaag
    28501 atagtcattg ctcctttagc ttttattcgt gggagaagcc tttcaaatat ttgtttcata
    28561 gtggatgaag ctcaaaatct cacgcctcac gaagtaaaaa ccattatcac ccgggcgggc
    28621 gaaggcacaa aaatcatctt tacaggcgat atttaccaaa tcgatactcc ttatcttgat
    28681 tcacagtcga atggcttgtc gtacctggtg gataagatcc acagccatcc catctatgct
    28741 cacgtaaaac tcgaaagagg tgagcgttcg gagctggcaa atcttgcctc agaattactt
    28801 tgaacttttt ttgatggcac ccctttcggc ttatgctgcg cttgaaccgg tctattctaa
    28861 tggatcaggt tgtcaaccca aatcatccag tagaaaaatc ttcaatggca gttgtttcga
    28921 tacgcccaaa ctgagacaat cggcgaaaag agatggtaga gcaaagcttt gctatacttg
    28981 gtcactgaat agaatgggta cttcgatatg atctccgcaa tgaactgtgc aacatatcaa
    29041 gcttattaat ctgcacatct tagcttgttc tcaaacagca catgcatcga atatttcgat
    29101 cgaagtgaag acagatagaa ttcactgaac ataaaaagcg attcctcaaa taatcttctg
    29161 cggtgaaaaa cggctaattc atttaatgtt agaaacctcc gaaggtttta tataaaaata
    29221 tcccaatggc ctgagaattt tatcgaatgt gaagaattga gccaacacta tatccttccc
    29281 gtcatttact tcggcataaa gtctgtcgac atcccaggga aaaagttttc cattttcgtc
    29341 tctaacttca tgctctggtt ttttatgaaa tgtccttatc gtggtgattt tcccattcct
    29401 gtccctgagg gtaatcacaa aaaaaggccg agaggcaagt agcgaatcca tatccaaatc
    29461 ttcgacatgg ttgaggaaag attcgaattt aatattattg aacgaattca taaaagccag
    29521 tactttaagt gtatcataac ccgaaacagt ttgttgggtg gcaagtgaga caacttcgag
    29581 gcgattgtcg tcgtgatgga ttactttata agattgttct ggcatttcgg gaatctcgac
    29641 ggtaacttcg tggatgtcgg ccagccgctg gtgaaagata gtatggtctc gccaatcttc
    29701 caatcgggta gaatatctgg gcgaaacaaa tcctctcaat cctggcaggt caacgataaa
    29761 gggcatattt gcaccttcca tgagcatata ggtgcctctt agatcgggtg tagcatcccc
    29821 cacataatac accttgcttt gtttttcagt gaaccatgcc tgtttgccaa aacaatgaat
    29881 gcggggcagc cattgataaa tttcgacctt gacggcattt gccgctaaaa gactaataac
    29941 atttttttgc tgattcgccg gaacaggctc ttttacccga atgttcttta tggtttgcag
    30001 aaacatgtcg ataacaggtt ttgaagcggg gtattctcca tttactgtcc agtgcccatc
    30061 ttcccgacgt tccagtatca ccgaatgatt tcgcttgtca gctaatagaa ttcttgaaat
    30121 actcgccgta tcttctacag caaatttacc ttcatcctta tcgaaggtgg tattcgaacg
    30181 accaaaaatc aaccaaccag cgattattgc caataaaact ataaggatta taatcaaaat
    30241 tcgtttattc ttcatgggcg atagttttct gtattttctt ttgtttcttt cgaattcatc
    30301 attatgattt gagaagatta accggttatg aactaactgt gggcaaaatt aaacatttcg
    30361 cttgttatga gtgcttaatt ttgtattcgc tcatcaagca aaaagaaaca tgaatttgtt
    30421 ctatcgtagc tttggtcagg gacctcattt actcattctt cacgggttat ttggactttc
    30481 cgataattgg gtgagtctag gacatcgata tgccgaaagt ttcaccgttt ggatacccga
    30541 cctaagaaac catggtcaat cgccacatca cccggaaatg ggctatctaa caatggtcaa
    30601 cgatattttt gattttttcg aacttcacca gatcgaaaaa agtgccatca tcggtcattc
    30661 catgggaggg aaagtggcta tgctttttgc tacacaacat cccgaaatga tatctaaact
    30721 cgttattgcc gatattggtc ccgggccgac tcccacacct aatttacaaa aaaccatcat
    30781 cgaaactatg tcgagcatcg atctatcgac aatgaaaaac cgcaggcaag tgattaaaca
    30841 attgcaagat acttttaccc agcgtggcct tattgatttg atattgaaaa atataggtcg
    30901 aaactccgaa ggcatatatt attggaaacc caatcttgaa gctttttatc agaatctcga
    30961 tgctttggcc gcaaatatca aattttctga ttactaccca aaccccactt tattgttgaa
    31021 aggtgaattt tcaaattata ttacagaaaa ggatttaacc attctttatg aacattttcc
    31081 tcttatgaaa gaagccatta ttcacaatgc aggccattgg ctacatgatg ataatccaca
    31141 agaattcttc gaagtgactt ggaaatttct cgattcctga acttactcat ctacgcaaca
    31201 aggttctttt tgaacgatac aaatatttat tccccaaatc aaaataagga aagttatatt
    31261 ttatacctac acttgtatag ataataaaat cgtcgggttc gcccgcaatc actccatcga
    31321 gcttatcagt gccaatgttc tggattatag tttcaaaggt taaatcccag ttcttatata
    31381 aataaacatt ggcgcctact ccataataaa tcataaattc catcttcttc tgtaccgtgt
    31441 ttaattcgat tttatttcca ctttcaaggt tatataagct gggacgcatt tttgttgaac
    31501 ctaccccaaa caacaaataa acgtatatat caggatgagg ttcgttagga taggccatga
    31561 gattcaccag atcgagctgc aaattggccc ctattacata agaataacct ttgagttttc
    31621 tattgaagat ttcgttgctg gtttgatatc gattgaaaat gaaacgacct tgtatggcca
    31681 gataggatat aaatttgttg ctgatgataa agccgctgct caatttactt tccttgaata
    31741 ttttgttgat cgggtcgttg tcatactggc ttaaatctcc aaaatacagg ccaggtccaa
    31801 tattccattg aaccgaccaa ttacgcttta aatcctcata tatcttattt tttccatatc
    31861 tacttgtttg agcaagaact ggcgtggtaa agtcttcaag gataaataca atgaataaaa
    31921 aacataccgt aagttgtaaa gttttaaaat gaggtttaat aggcattttt acagtttctt
    31981 atatccaaca aagtattaaa aaaaagtaga atatccagtc agtgtttttc tattgctctt
    32041 acaaaggaaa taaattttaa tgattcacga aaatgcattt ctatattcta aaaaaatgtt
    32101 aattatcagg aaattataat aaaattcata aaaatgaact tattttcggt tttttgcagc
    32161 caatgttgcc acataaatac ttatctcata gagaattaat aaagggatgg ctaccattat
    32221 ctgactgaac atgtcgggag gtgtaataat tgcagctact ataaaaatca ccactatcat
    32281 tacttttcgg tatttgcgca tccatcggtc ggtaatgatt cccaagcgtg aaagcaccca
    32341 ggccagggca ggcaattcga agatgattcc caccgacagg gttaccccaa ccacagtgct
    32401 tatgtaagat gaaagtgaaa cctgatttct taccacttca cttacttgat aactccctaa
    32461 aaagttaatg gttaaaggaa ctatgaggaa ataactgaac ataattccca tgataaagag
    32521 acagcttaca atggttatag ctatgctgga ataacggagt tcttcttcgc gcagggcagg
    32581 ttttataaac ctccagagtt catagaatac ataaggtgag gcaatgatga ggccagctat
    32641 cagagagata taaatatgtt gcgtaaactg gcccgacatc gaaatattta taagcgacaa
    32701 tttgctgttg tcgagacata aagcacttat ccccagtttt tctcccaaac tacacaaaaa
    32761 cctaatcgta atgaaatcat tgctttttgg tccaagtaat accgtatcga aaatgaaatc
    32821 tcgtttcatg aaagcaaagg tggcgcccaa ggcgacggca ataatggagc gtaccaaatg
    32881 ccagcgcaat tcctccagat gttcccaaaa ccccatctct ttttgggggt ttgatttctt
    32941 tttcattttt gatgggctag catccaccat tttattataa cttgcgtcaa aattacgaaa
    33001 ttctaaggcc ttgatgaatt catccgatga ttaacacttt ggaaatatgt tgatggaaga
    33061 aaattttcgc aaaaagacag cccatgacgt ggctcgttta atgatggctg cagcgcgcac
    33121 agctcctaaa ggcagaggtc gcgattcatt attcattgct ttagccagtg atgacgaaat
    33181 gcagcaaatt gcaaaaacca tgaaaatatt tgccgaagaa tgggaaacac aattttttgc
    33241 acgtgatgcc cgaaatcttt tgcattccga cgcgcttgta ctgataggga caaaaatcga
    33301 acctctcgat ttgagaaaat gtggattttg tggtttcgaa aattgcagtg aaaaaaatct
    33361 acaccccaat actccttgtg cctataacaa tatcgatctg ggaattgcag tgggttcggc
    33421 tgtagctgtg gcagctcagc atcacgccga caacagggtg atgtattcgg ttgggaaaac
    33481 ggctttgtac atgggtctgg cgaaacctga tgtaaaaata cttctgggaa tccctctcag
    33541 tattcgttct aaaaatatct acttcgatcg ttagaaacgc atcaactttt taacttctga
    33601 aggaagaacc aaatttttct ctcttaattt cagcacgaaa attccagcca aaatggcttc
    33661 ggcagtatta tcgtcaagac gtgatgaaac ctttttggca cagcatgaaa ccacaatttt
    33721 ggccaattct atggcctttt tcttggcttc gctgctatgc agcataatgg cgggattaaa
    33781 aaaacaactt acgccattgc tgagcatgaa tcataagaac ttcggcttgt tgccttacag
    33841 cttccaacag ccagatctcg gctaattctc cccctccttc cagaacaagc agttttaccg
    33901 tttcatatct tttcaaaatt cgaggtatat ttctttttaa acgatttata tcaccataat
    33961 tatgtgatcc atagaaaagt agcttgcctt caaagttaaa aactgccatt ccaagtttta
    34021 caccagtatc gatggagatc agggtaccca tgtctaagtt taaaaattta ggtaaaaatc
    34081 ttcggtaagt ctcctatatt cttgagtata agaattcatt ccagcaggat gtatgcttaa
    34141 agattcgctc tggcaagctc tgtccgaaag cgaatattcg gcaagcacga gatagggatc
    34201 ataaccctct ttgcttgaaa caaagagttt tcgtttagga atgaacccat tatccattgc
    34261 catttcttcg aagtacctgg cttcaaatac gggcagtata atgccaaaac tacctttgtc
    34321 attcagtaat cttttacaat ccacaagcat gagtttatgc cggaactcat cggtatgtct
    34381 tgcgagattc ctaacaggat tttttacttt taaactattg ataaaaaaag gaggattggt
    34441 gataatgtaa tcaaaggagt tcccttcgta atcttcgagc gcaatgtgtt ttgctttcaa
    34501 tctatttttc catcgacttt ttttaaaatt ttcctgggct tgttcaatcg aagggaaatg
    34561 aatatctata cctgtaatcc atgccttgct tctctgagca cacatcaaag aaagcaatcc
    34621 gcatcctgtt ccaatatcca agatccgctc agcttcacct atagctaccc atgcacccaa
    34681 tataaccgag tcggtgcata ccttcatggt actctggtta tcttctatct cgaactgctt
    34741 gaaacgaaac attcttatga taactatgat aataacagtc tttattcaaa taggaaccaa
    34801 atttcgaaag ttttcggaag ataactttcc gcactttttg taggaaaacg atttcacggt
    34861 tttgaacttg tcttattgat tcaggaaaag caaatcatca ggaatatctg ttttgattga
    34921 tttattgtta aagtcgatat tctttataaa atgctcattg atagggagga ggacttccat
    34981 attctgaaaa tcgaaaatta ccagagggtt atcgggagtg ccgccaaggc gttgtacctc
    35041 gccaagttta cctcgatgaa tgtcgacgat tgtaaatccc tcaattcgtt gggcaggaaa
    35101 tcgtgttggc catatcgagg gaaaatagct tttatgaatg taataggaac gtttgcataa
    35161 aaaggaggct ttcccaggat cgggaatatc ttccagataa ataatccagc ggccacttgg
    35221 tccgggttct gaattcagta tatgaaaggg aacaaagcta ccatcaatgg caacgaacat
    35281 aaagtctatt cgggcgtgtt cgaaagggtt atagtttaac tcgactgaga ttgcaagtgc
    35341 gcctgaaatg ccataagttc ggagtatttg tccggttttg atatagtctt cttgctcgat
    35401 catgaagtga ggattcaagc ttctaaatta acaaaaaacc cggtgatggc cgaccgggtt
    35461 caaagtatca ccgaaaagat gttattcttc agttgagggt gagatttcag cttcttccga
    35521 attcgaaagt tcctcgttga gggtttcagt actgcgtttt ttggcaattt gccttgctct
    35581 ttcttcggca attttgcgtt cagcttccag tttgcttttc agatgatcac gctggaaggc
    35641 ttctttgctt gtaatcttag ccttgatttt ggcttctttt tcagcagacc attcctgaaa
    35701 tttagcttcg gcctgttcgg cagtcaaagc tcccttttca acacccctta acaaatgtct
    35761 tttcagaagt acacccttat atgacaataa ggctttaacc gtatcagaag gttgagctcc
    35821 gacgttcaac cagtaaattg ctctatccat atcgatctgg atttcggcag gattaggaag
    35881 ggggttatac gttcccagtt tttcgataac acgtccatcg cgtggtgcac gaccatctgc
    35941 tactacaata tgataaaaag gctgaccctt tttaccaaat cgttgtaatc tcattctaac
    36001 agccataaat tattgttttt taaatggtaa taaaatatct tgtttgcaaa aaacaagggt
    36061 gcaaatttgc aaaaaaaatt taaattctct taaataatcg gtattttcct tttaaaaatc
    36121 atcatccgtc tgaagcaatc gttcactgcc tggtccttta atcaagccac gtgaagagcc
    36181 ttgaatgaag ttcaagatgt tttgaagttc gggtgaaaag ggaatttctt cttgaatggt
    36241 ttgaagagca tgtgaaacat tcaatccttt caaataaata attcggtaga tatcttcgat
    36301 gagtcggatg gtttcgagtg ggaatccccg ccggctgagg cctatggagt tgacacccgt
    36361 aaagatcaat ggttcacgtg cagccttgac ataaggtgga acatcttttc ttaccaatga
    36421 accacctcct atcatactat gagctccaat acgtacaaat tggtgcactg ccgacattcc
    36481 gccaacgatg gcccaatcga aaatttcgat gtgaccggct aaattcactg cattggacaa
    36541 aattacatgg tcgtgaatat gacaatcgtg agcaatatgc acataggcca tgatcaggca
    36601 atcattccct accacagttt cgccgctggc tttcgttccc cggttcacag taacgaactc
    36661 tctgatggtg gtattgtcgc caatttttac taaggactct tctccattga atttcaaatc
    36721 ctgtggtatg gccgaaatca ctgctccagg aaatacccgg cagtgcttgc ctatacgggc
    36781 gccttccaaa atggaaacat taggcccaat ccatgttcct tcatctattt cgacgttttt
    36841 atgtatgcag gtaaaaggtt cgatcaccac tgaaggatgg attttagcct cgggatgaat
    36901 actcgtaagt ggttgatgca ttttttgcgg attaatttgc aaatgagtcc gggattacta
    36961 tattcatttt aaacaactta aataacttaa acaacttcga caaaaataat taattcgaag
    37021 gagtgggttt tttggaaatc tgtgcaagca tctcggcttc catgactact ttgtcaccta
    37081 caaaagcttt ccctctcatg tggcataaac cccgccgtat tggcgaaata agttcgagag
    37141 aaaaaataag tgtatcaccg ggaactactt tatgcctgaa ttttacctca ttgatcttca
    37201 tgaagtaagt gagataattt tcagggtcgg gcacagtact cattactaaa acgcctcctg
    37261 tttgagccat ggcttcgatt tgtatgactc caggcatgac gggctcgtcg gggaaatgtc
    37321 cggcaaaaaa cgactcattc atggttacat tctttaagcc aacgacatga gaatcagtca
    37381 tttctaatat tttatcaatc aataaaaaag gaggcctatg gggtaaaagt tttttaatgt
    37441 cgttaatgtc gagcaaaggc ggcttattgg gatcatattg aggagtatgt tccgtttttc
    37501 ggtgctcttt caagtaagct tctctcattt tgcgcgcaaa ttccacatta acgacatgtc
    37561 ccggacgagt agcaagtaca tgggccttga atctgaaacc tgtcaatgct atatctccca
    37621 aaagatccag gagtttatgg cgagcaggct cattcataaa atgtaactcg agattgttca
    37681 aaacgccttc ttcgagtacc ttcacttcag gcctgtcaaa ataaatggca agccttttca
    37741 gttcttcctc tgacagaatc ctgtcaacca atacgatggc attgtttata tcgccacctt
    37801 taataagatt atgctggatt aactgttcca gttcgtgcag aaagacgaag gtacggcagg
    37861 gagctatttc ttttgcaaaa tcgtctatcc tgtcgagagt ggcaaattgt gtatccagaa
    37921 ccttcgagtt gaattgaatc attgccgaga aacgttgaat atcagaagga atagcaatga
    37981 tttccgactt tcggtcggat ttggtatgaa ttatattgtg tttgagttcg aaatatacac
    38041 gttcggcttt ctgatttatc aaacgggctt tatgaatcaa atcgacccag ggagccgaac
    38101 ttccatcgag aatgggcact tcattcccat ccatttctat taatatattg tcgattccac
    38161 ttccgtatat agctgcaagc aaatgttcaa ctgtactgat gtgtatatta tctttttgca
    38221 aattggtgct tctgtcggta ttgtttacgt agtcaaccaa agcttctatc tgtggatacc
    38281 cttctatatc gctacgaacg aatttatatc cataatttat aggggcgggt ttcagagtta
    38341 gggtaacaaa ttcccccgta tgcag
//
LOCUS       DAOORU010000006        37447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_1273_length_37447_cov_6.021662, whole genome shotgun sequence.
ACCESSION   DAOORU010000006 DAOORU010000000
VERSION     DAOORU010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 37447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37447
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1273_length_37447_cov_6.021662"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(442..1899)
                     /locus_tag="PLF20_01055"
     CDS             complement(442..1899)
                     /locus_tag="PLF20_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005789241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOX79630.1"
                     /translation="MDQSLTSLQPQNVWHYFEEICKIPRPSKNEAQIRNYLLEFAKLH
                     HLEANTDDAGNVVIRKEAKPGFEDKPIVILQSHMDMVCEKNQDSLHNFEIDAIQPYID
                     GEWVKARNTTLGADDGIGMAAQLAILESDDIPHGSIECLFTVDEETGLTGASAIQPGF
                     LKGRILINLDSEDEGELFIGCAGGIDTLAELQFKRRVVPVNYLPYRIKVTGLKGGHSG
                     DDIHKGRGNANKILNRFLWNASNRYNIRLAQIDGGNLRNAIPREAEAVILVPFKTENM
                     LLEYFKEFYTEMVEELKYTEPHFCLTLESTTHPEAIINKKSQQRLLNAIYACPHGVIS
                     MSQRMPGMVETSTNLASIKITGPNTLAVNTSQRSDLESAKTDIASMVESVFLLAKFKV
                     THSDGYPGWTPNPSSRILNTTIQSYKNLFGNEPRVRSIHAGLECGLFLKKYPYLDMVS
                     FGPTLRDVHSPDEKIHIPSDDKFWKLILETLKNVS"
     gene            complement(2279..3571)
                     /locus_tag="PLF20_01060"
     CDS             complement(2279..3571)
                     /locus_tag="PLF20_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOX79631.1"
                     /translation="MNDTNIVYPNLFQRNRKKLINLLPQHALVCISSNDLMPRNGDQY
                     FPYRQNSDLFYLTGIGQEKTILILFPSHADEKMKEILFILRPDETTEIWEGQKLRLEE
                     ASQISGMANIRYTDQFEATLKDLMVLTSNVFIDSNEYPKFFPEVKVNEHRFALKMRKM
                     FPLHTFSRLAPLLWKLRLNKEPEEIEFIRRASQLTGNCFRKILKITSPGMFEHEIEAE
                     ITYEFARNGCCHAFQPTVASGANACYLHYRSHQQKLEKGDLLLLDFGAEYKNYASDCS
                     RTIPISGKFTPRQLQVYEAVLRLQRHALSMLVPGNTINQVNDELNHLAEKEMINLGLF
                     SEDDLLHQDPQNPLRTKFFMHGVSHFVGLDVHDVGSRDEVFSPGMILTFEPGLYIPEE
                     NIGIRLENTFVVGYPSYNLMEDIPIDPDEIEELMIKPK"
     gene            3911..4594
                     /locus_tag="PLF20_01065"
     CDS             3911..4594
                     /locus_tag="PLF20_01065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HOX79632.1"
                     /translation="MSKTLLRMTSLSKKVFVSLAGLFLITFLLLHLSINLFILPITPN
                     HEETFRQAVHFMTTSPLIKVMEVVLFAAFIIHIFFAVTLQIENWIARPRGYKVWQKSR
                     TDSPFSKLAIYTGIFVLIFLIIHFMNFYFVKQGWVSPPEGAYEVADKHDFYNMALNLF
                     NNPVYCVLYIVLIIVLGFHLHHAFQAGFQSLGLNHPIYTPIIKILGWIYSILVPLGFI
                     SIPLYFLIS"
     gene            4613..6556
                     /locus_tag="PLF20_01070"
     CDS             4613..6556
                     /locus_tag="PLF20_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HOX79633.1"
                     /translation="MTQLNAKIPQGSLQEKWTNYKANQKLVNPANKRKFEIIVVGTGL
                     AGASAASALGELGFNVKVFCIQDSPRRAHSISAQGGINAAKNYPNDGDSIWRLFYDTI
                     KGGDFRSREANVYRLAEVSNNIIDQCVAQGVPFARDYAGYLDNRSFGGAQVSRTFYAR
                     GQTGQQLLLGAYGALSKEIKRGSVKMYTRHEMLDLVIVDGIARGIITRDTITGKIERF
                     AAHAVIIATGGYGNVYFLSTNALNCNASAIWNCYKKGAFFANPAFVQIHPTCIPVHGD
                     YQSKLTLMSESLRNDGRIWVPKKKEDVEAIRSKKLRAVDIPENDRDYFLERRYPAFGN
                     LVPRDVASRAAKERCDAGYGIGPTGLAVYLDFSESIRRLGRDVIESRYGNLFQMYEKI
                     VDENPYENPMMIFPAVHYTMGGLWVDYELQSTVPGLFVAGEANFSDHGANRLGASALM
                     QGLADGYFILPYTLQNYLADQIGVQKFETDLPEFELAEKETIEKLQRLLAIQGKYSVA
                     TLHKKLGKIMWENVGMARDEARLKQAIEAIRSLKQEFWKEVYVPGSLTGVNDELLKAN
                     RLADYLELAELIAHDALLRNESCGCHFRVEYQSEDGEALRNDEEYKYVSCWEYQGEGV
                     EPQLHKEPLEYEFVELKSRNYKV"
     gene            6587..7372
                     /locus_tag="PLF20_01075"
     CDS             6587..7372
                     /locus_tag="PLF20_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HOX79634.1"
                     /translation="MNFTLKIWRQENSKAKGRFKTYKIENISPDCSFLEMLDILNEKL
                     IHDKIELPVDPVAFDHDCREGICGMCSLYINGHPHGPEQGTTVCQVHMRKFQDGDTIV
                     IEPWRAKAFPIIKDLMVDRSAFDKIIQEGGYISASTGGVPDANSILVSKEYADMAMDA
                     AACIGCGACVAACKNASAMLFVAAKVSQFALLPQGHPEAKRRVQAMVSKMDELGFGSC
                     TNTYACEAECPKNISVEHIARMNREFLKSKLTHAEKKRIGGFG"
     gene            7751..7924
                     /locus_tag="PLF20_01080"
     CDS             7751..7924
                     /locus_tag="PLF20_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79635.1"
                     /translation="MITRVMDRGAFKRRIMGMAFIDGREASRQASKGFKRAPLRDPIE
                     YSVKSNHESKKRD"
     gene            8496..9083
                     /locus_tag="PLF20_01085"
     CDS             8496..9083
                     /locus_tag="PLF20_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX79636.1"
                     /translation="MRQVLVFTVYVLSFLWLSWMDGIDNLNAQSYYGFHPRMCLASYP
                     ELNHENYYPPGRDSKGEDIDANHEDAEWFAPEYFFQYSIDKNTGKLTVSCALEKVEVE
                     HNRERGDIDSQSEDSCRCSSSIVIIPNPANDEVILKGACNSSVYVFDFKGRQLLFSRI
                     EDIPRLDIRSLNTGIYSIKVECAEDIYTLQLIIQR"
     gene            complement(9087..10205)
                     /gene="hydE"
                     /locus_tag="PLF20_01090"
     CDS             complement(9087..10205)
                     /gene="hydE"
                     /locus_tag="PLF20_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOX79637.1"
                     /translation="MNPKPSIRHLLGKKTFSHDELVILLSADKGEQNLIFQKASEVKQ
                     NNVGNKVYLRGLVEYSNICSKNCFYCGVRSGNKNVRRYKLSESEVLEAAQFVLDQHFA
                     SMVIQAGELSSPQNTCRIGELVRKIKQLSQGKIGITLSLGEQSREIYRYWHDCGAHRY
                     LLRIETSNPDLFQKMHPQDTFHTFEKRLQALEDLRNEGFQVGTGVMIGLPFQTYDDLA
                     FDLEFFKNLDIDMVGMGPYIEHKDTPLYQYKDLLLSPLERLDLSLKMVALLRIIMPDI
                     NIAATTAMETIDPNAKIRALKVGANVIMPNLTPLKYKEDYLLYEGKPNIDEEAQQTLN
                     YLIQSISAIHCEIGWDNWGDSLHFFHRHPENKEIEPHS"
     gene            10379..11896
                     /locus_tag="PLF20_01095"
     CDS             10379..11896
                     /locus_tag="PLF20_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOX79638.1"
                     /translation="MKILSVDKIREADAYTIANEPIASIDLMERAARQCSQWLLDHLV
                     KDMPVMIFCGTGNNGGDGLAVARHLFENGFKVSVYILRFGSQASPDFLTNLCRLESLP
                     VECMDIASAGDFPEIPPFSYIIDALFGTGLSRKLEGLAVELVSYLNHSRGIIISIDIP
                     SGLFVDTSLKAVSGIAVQADHTLSFLPPKLCFMMPENDIYVGEWHVFDIGLHPEYLQQ
                     AETKYFYSSKDDVTSWLKRRRRFQHKGDFGHALLIAGSEGKAGAAIMAARACVHSGAG
                     LLTVHAPGKVGEALQINLNEAMVSFDEQASFITHLPSFDRYDAVGAGPGLGLHRQTQT
                     ALKLLIQNYRGPLIMDADALNILSENKTWYSFLPPYTILTPHPGEFERLAGKTDNDFE
                     RLKLAKDFALKYNIFLVLKGGVTIIVNPLGQCYFNSTGNPGMATGGSGDVLTGIILSL
                     LAQGYNHGQASVMGVFLHGFAGDLAAEELSQEYMSAVDIIRFLAPAFQSLSKGRG"
     gene            complement(12005..12535)
                     /locus_tag="PLF20_01100"
     CDS             complement(12005..12535)
                     /locus_tag="PLF20_01100"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOX79639.1"
                     /translation="MSSPLAFIFDMDGTIVRNHENHLNAWKLFCQNHHIAITEEELLA
                     VFGGKNEDILQKVFVHKLSQKELRQFEEEKEGLYRGLYKPYIKEVEGLSDLLKNLFIA
                     QIPAALATSAPTSNVDMVIDALDIRKYFSCIIDATMVTHGKPHPEIFLKVAECLREKP
                     QNCLVFEDSRVGIERQ"
     gene            complement(12537..14498)
                     /locus_tag="PLF20_01105"
     CDS             complement(12537..14498)
                     /locus_tag="PLF20_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79640.1"
                     /translation="MLQKKSISFLCLVISFIVGKQVFSQEIPCSQIRDDTLWLCCGTK
                     ILLDNELVLISTDTIIIGDDISRVYFLKDPYARSRILYDSLKSKAGKTELTKLAYQLL
                     FPESGAVSKNNETLSEPPEENEYLPFADRTIQKIIIYVDKPFDETQPNLTWARLSRMG
                     NSLHRTTRKTVIRNNLLFKEGEKLDPYIMAENAQLLRSLPYFSEAYLRPVADEKNPEK
                     VDVILWAKDALSLGLDIYLPSSTLVDFSLFDQNFLGYGNRLSSTVRYNTAKSPVFSLK
                     NLNYRIENIKGTFFYSEADYTNEFNHESIRLTIARDFLTTQTPFASGLSLTHHEKIQN
                     IFTPYFRQDRLKYFNPSLWIGKAFNVYGDGQPTQLVISSSIDSRLYTQRPYVSADSNK
                     IYYNSSNILLNIAISRNAYYKGNYIYQFGRTEDIPYGFLAELTTGPESYEYYNRTYFG
                     FTTGVAKYFNRLGYLSGKFLISSYNRSGKFEQGQWGTHLEYFSNLYIYGPFKLRHFLT
                     LNYCQGMNRLEGESVNVVKELDLLIAQPSDTAFYSGNEKLTLNYSSTLFTPYYFYGFR
                     LAFFDFVNLAYLCGRQQFPEKNKFFSGFGIGAMVRNENLVIKTILLKLGFYPGLPDNR
                     SGVFIEFSGIAPLNFFNYKPHQPLISKYE"
     gene            complement(14574..16313)
                     /locus_tag="PLF20_01110"
     CDS             complement(14574..16313)
                     /locus_tag="PLF20_01110"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HOX79641.1"
                     /translation="MNKINPAILEKAHHWTQPPFDEQTRQKVQYLLDNDPAELIESFY
                     TDLEFGTGGLRGIMGPGTNRMNIYTVGMATQGLANYLKMNFPDKNPIKVVIAHDCRNN
                     GRLFAQTTANVLSANGIKVFLFDDLRPTPELSFAVRFLGCQSGVVITASHNPKEYNGY
                     KAYWEDGGQLVSPHDKNVMAEVSKIKGIEEVKFEGNSRLIETIGEAVDRAYIAAIKNL
                     SLYPEIIARYKNLKIVYTPIHGTGVRLAPWALQEFGFEQIFTVDEQMMVSGDFPTVHS
                     PNPEEPAALDLAIKKARETGADLVMGTDPDGDRVGIAVKDEKGEFVLLNGNQTASLLI
                     YYILKAWKAKRRLKGNEFIVKTIVTSELIKEIAQKEGVKCFDVLTGFKYIADLIRRLE
                     GSMHFIAGGEESYGFLTGDFVRDKDAISTCCMIAETAAWAANQGKSLYRLLKEIYLEY
                     GFYKETLLSITNKGIAGAEEIRQMMENYRANLPEHLAGIKVTVIKDYLCQTEINLITG
                     EKIHIDLPKSDVLQFFLEDGSKITVRPSGTEPKIKFYFGAKTILHHIEDYEKTNQFLS
                     QKIEDIKHDMGLA"
     gene            complement(16316..16702)
                     /locus_tag="PLF20_01115"
     CDS             complement(16316..16702)
                     /locus_tag="PLF20_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOX79642.1"
                     /translation="MNNTDRNRIIAQIESMMNQIWEKLGEGLPLATYRECLIFELRSA
                     MLSFRENVVIPVTYKYFKPENQLKVDLLIENEIPVEIEMESSEAHRKMMAVLRHSGKP
                     IGLLITLQVKPVRFILNKITYPQKIR"
     gene            complement(16695..17561)
                     /gene="rfbD"
                     /locus_tag="PLF20_01120"
     CDS             complement(16695..17561)
                     /gene="rfbD"
                     /locus_tag="PLF20_01120"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOX79643.1"
                     /translation="MKTKILVTGSNGQLGNELRLLSAHLNHLSFVFTDVTELDITQPE
                     AVNEIMESLQPQYLINCAAYTAVDKAESEKESAWKINAIAPEILANSARKTGTCLIHI
                     SSDYVFDGQNYRPYLPSHPTHPLSWYGKSKEGGEIAIMRSRVNAFIIRTAWLYSSFGS
                     NFVKTIRRNALQKQNLDVVSDQVGSPTWAADLAEMILHVIIHRIEPEGVEILHYTNEG
                     VCSWYDFAISIVEHSDISCTIHPISSEQYPLPAPRPFYSVLDKTNTRQRLNIEIPHWN
                     ASLKKCIQLLNE"
     gene            complement(17605..18543)
                     /locus_tag="PLF20_01125"
     CDS             complement(17605..18543)
                     /locus_tag="PLF20_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX79644.1"
                     /translation="MRKKILVTGGAGFIGSHLCKRLLDLGHEVICLDNYFTGSKENVV
                     ELMSNPYFELIRHDVTMPFFIEADEIYNLACPASPIHYQYNPIKTIKTSVMGAINMLG
                     LAKRIRVKILQASTSEVYGDPDVHPQTEDYWGKVNPIGPRACYDEGKRCAEALFMNYY
                     RQNNVRIKIIRIFNTYGPKMHPNDGRVVSNFIVQALKNQNITIYGDGSQTRSFQYVDD
                     LIEGAIRMMDTPDDVTGPVNIGNPDEFTILELAEKVIRLTGSRSKIIFQPLPPDDPKQ
                     RQPDITLAKNLLNWEPKVALEDGLQMTIEYFRKLIR"
     gene            complement(18612..19943)
                     /locus_tag="PLF20_01130"
     CDS             complement(18612..19943)
                     /locus_tag="PLF20_01130"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005817149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOX79645.1"
                     /translation="MKITIVGSGYVGLVTGTCFAEVGIEVTCVDIDSRKIENLRKGII
                     PIYEPGLEPIVMRNIDKGRLFFSTDLKSVISQGTDMVFTAVGTPPSGDGSADLQYVIN
                     VAREIGRYMQDYLLVVTKSTVPVGTSEKVRHVIQEELDKRGVNIPFDVASNPEFLKEG
                     DAINDFLKPDRIVVGIDSERAQDIFERLYKPFTLNGHPIIFMDIPSAEMTKYAANSML
                     ATKISFMNDIANLCELVGADVNMVRKGIGSDSRIGTKFIYPGIGYGGSCFPKDVKALI
                     RTAAEKGYQLSVLKAVEEVNENQKLVLYKKLNSYYSGQLNDKTIAIWGLSFKPNTDDM
                     REAPSISLIHQLLSVGAKVKAYDPVAMNEARQCYFGDQIEYSKDQYEATIDADALLLV
                     TEWPEFKFPNFNVVKKLMRHPVVFDGRNIYSKQEMKDMGFDYFGIGIPSLK"
     gene            20148..22031
                     /locus_tag="PLF20_01135"
     CDS             20148..22031
                     /locus_tag="PLF20_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOX79646.1"
                     /translation="MQMTRTFDLLDQWIEKYPRPDVFGAKRNGRWIHFSAVDYKEFSE
                     SLCMGLMALGFVQGDKIATITANRPEWNFVDMGLALGGFVHVPIYPTLNVEEYYYILE
                     HSDTKLVFVGDKSLYAKIKPIADALPQIQMVVTFNEVEGAPHWMEIVETGRKMREKYD
                     GPLKKIKNSIKPDDLVTIIYTSGTTGKPKGVMLSHWNIMSNTIATSKINPLTHEDKVL
                     SFLPLSHIYERMMNYDFQYLGIGIYYAENMGSIVANLNEIQANEFNAVPRLLENVYDR
                     IVAQGKDLNGIKKAIFFWALHLAERFEFNNANGWFYDLQLKWADKLVFVKWREALGGH
                     IKVIVSGGSSLQERLCRIFWGAGLPVVEGYGLTETSPVIAVGHFNWPDIKFGTVGPVL
                     EGVEVKIANDGEILVKGPNVMLGYYKDPEYTAQVIDEDGFFHTGDIGVLVDGKFLKIT
                     DRKKEIFKLSAGKYIAPQVIENKFKESIFIEEIMIIGENEKFVSAFIVPNLNYLHFWA
                     SKHKIHYHDNRELIKMEEVIERIDEEVQRLNKTLSLHEQVKRFRLVADEWSPQTGEQS
                     PTLKLRREVIYAKYSSLYEEIYGHPRLSKNNGKNSEPHKPSKYAFNLNLGLKFKPKDK
                     SEE"
     gene            complement(22070..23128)
                     /locus_tag="PLF20_01140"
     CDS             complement(22070..23128)
                     /locus_tag="PLF20_01140"
                     /inference="COORDINATES: protein motif:HMM:NF023179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOX79647.1"
                     /translation="MKRRTILLALIFILEAKSYLSSQPAAYTQWWNAPQQENPALTAF
                     GIEKYRLDGVYQTRSYENAPNLNTLYLSASYRFNFENTNKEYGLVLPRSNPLSIGIGI
                     FSERRFADTTQYPYAIIGASFDILAGFGGTNTLAVGLQPYWFESEGYYPVSKIYFQSN
                     DTLLPGHSPHKKFGVNVGILLGINKMDCWTDDQVHRFEIGMAIYRLSMPYRTLIPDIH
                     PGKEIHSHAGYLLPLSQKLGMVFRTSWIYEGQHLYNGGFSLIYRRHFAFADRFRMGIY
                     YSNTYHLSLTAGFRIYTPKTSTLSLDLEMSRDFALKNQQNMIPWHQNAWEIAFIVKPI
                     KKCWSLDNCSRAYQYEGF"
     gene            complement(23312..26395)
                     /locus_tag="PLF20_01145"
     CDS             complement(23312..26395)
                     /locus_tag="PLF20_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOX79648.1"
                     /translation="MSFIADLHIHSHFSLATSKELTPEYLHLWARRKGIDVIGTGDCI
                     HPNWLSELQEKLEPAETGLYRLKTIYRLAEENIPESQEPLFLLTTEISNIYKKGGKVR
                     KVHNVLMIPDFRTASLIQQKLKLLSFNITSDGRPILGMDSYELFRLVLDINPQCVLVP
                     AHIWTPWFSVLGASSGFDSIEECFEDLTPHIHTLETGLSSDVPMNRVVGKLDRFHLIS
                     NSDAHSPDRLGRNANIFFCDKSFDAIIEAMASHRSETIDLFPQEGKYHFAGHRKCNVS
                     LPPDEAAYHQGICPVCYKKLTSGVLDRVFQLASKTLPDEESISPRFHYIIPLPEIIAE
                     IIHTKSASKKVMEKYFQILKILGPELKVLLEIPCSQIAEHAGGELAEAIERLRQRRVM
                     ITPGYDGEYGTIKVFRPGEAQSYAQKSSLFHDENPLNRPTAYPLLSFNIHHVLEQKIR
                     YSAIPDSSSEQDTVYPTTDDLQKAAIAHDEGPAIAIAGPGSGKTYVLTERIARLISQN
                     IAPSEKILAITFTTRAAQEMRQRLMSHPSLHQQASSLSISTIHALGLQILREIPSYSS
                     FKLISETEKIKIIQSLPQVQNYSPVKIVNYISLLKNHALNFRFDVTFQKIANQYQFYL
                     LNNQLMDYEDLILLPLNFLQEDAEFRSQIQNRNGFILIDEFQDVNPAQYEFLKIISHP
                     QENNVFAIGDPFQSIYGFRGAYSGIFETFLQDFPQATTYHMGKSYRCGDKILKAAYEV
                     IGSENPLEGLPEKGKVNIVQHATDKSEAEFIARSIDAMTGGTRFFAYDSGVAQAQTEI
                     PLSEFTILCRTTRQFPAIIAALNNHGLPYQTAQAGNLLDSELLSEIILYLRKILENPE
                     ENKCTVENLLKAYFEQNLSEKNDTIDNRNIFMEIAKDFGNDLKSFIDFIDLNAPTGLY
                     EAKENAVQLMTLHAAKGLEFEHVFISGCEEGLIPYSLFENQETDPEEEKRLLFVGMTR
                     AKKTLTLNYADSRKIFHRNMKLPPSHFLAAISPANAIRTKSNLPEKHEGQQLSFF"
     gene            complement(26712..28781)
                     /gene="fusA"
                     /locus_tag="PLF20_01150"
     CDS             complement(26712..28781)
                     /gene="fusA"
                     /locus_tag="PLF20_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017141367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOX79649.1"
                     /translation="MQLHKLRNIGIAAHIDAGKTTVTERILYFTGANRKMGEVHNGEA
                     TTDFMKQEQERGITIASAAITCKWKEHHINLIDTPGHVDFTVEVERSLRVLDGMIAVF
                     CAVGGVEPQSETVWNQANRYKVPRIAFINKMDRQGADFENVVAQMNKYLDANPIPFQI
                     PIGNEEDFKGIIDLIKMKALFFENFESTEAEIPAEYKGSASRARQYMIEKLADFNEEI
                     LEKYLNEEPIPEELIIEASRYTTLHLLATPVLCGAAYKNKGITQLLDAVIHFLPSPLD
                     VGAVVGMDRTDPEKTHSRYPSPKDPFSALAFKIIYDHYVGQQTFIRIFSGRLSTGMQV
                     LNSSKDKNERIGRIMRIHAKMREDIPEAGPGDIVALVGLKFTKTGDTLCDPDNPLLLE
                     SIFIPPPVIELKVNPSKQSDQQKMGEALHKLASEDPSFIVRFDDETQETIISGMGELQ
                     LEIILDRLKDEFGLELQTDEPSVAYRETITHETEQAYKHVKQTGGKGQYAHVLLRIEP
                     NPGKGYEFVDKIKGGIVPLEFIPSVDKGIRKTLEKGVIAGYPVVDVKVTLLDGSYHPV
                     DSSDFAFQTCASICFKQAFQKAAPILKEPVMKIEINSPDDYIGNVITDLNKRRGQIES
                     MRRHRKGSQKINGLVPLMEMFGYATQLRNISSGRANYSMEFFQYMPLPQTLQEEVIKK
                     AIEKNKK"
     gene            complement(29314..29868)
                     /locus_tag="PLF20_01155"
     CDS             complement(29314..29868)
                     /locus_tag="PLF20_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79650.1"
                     /translation="MDRTAHLFCLLIMISIAACKTPSSPDEKEYYDQILEKQNTLADA
                     IFDLNRYLEHADTAGLQRAYNKTLDYAKNSLQEIEKIGDYQGDTILLKATLSLLKVYC
                     EVLENEIQEMIAIVKKPGEISDADDARIMRLRKNIYKKLTQAEREFVKADHHFRNLYL
                     GAYLSNEPDTTLFEELNDSVPATP"
     gene            complement(29861..30322)
                     /locus_tag="PLF20_01160"
     CDS             complement(29861..30322)
                     /locus_tag="PLF20_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOX79651.1"
                     /translation="MKIQNQEIRYQHIYGQLETLLTKTKVPEARMSTIIAVLHHKMPN
                     FFWTGFYHLVDDELIVWCYQGPLACQILEKNKGVCWAAIQQQKTIIVPDVEQFPGHIA
                     CDSRSRSEIAVPLKDAGGNIKGVLDIDSQLIDNFNETDAKWLEKIIDMVYG"
     gene            complement(30443..31135)
                     /locus_tag="PLF20_01165"
     CDS             complement(30443..31135)
                     /locus_tag="PLF20_01165"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOX79652.1"
                     /translation="MMIPTQDIHSYVSALSSIFEEEGNRETASGAKAYMRNQFEFYGL
                     PSPLRRRLQKGFLLQNGYLSIEQWPELIFQCWNSAYREMQYFAMEYSQHFIPHCTEEH
                     IVLLEWMMTNKAWWDTVDFISPKLCGKFFIHFPDIIENTTSKWIDSQNLWLQRAALIF
                     QLNYKEKTHLDLLFRSIQCLSDSDDFFIQKAIGWALRQVSKTFPEEVMHFVAHQPLKP
                     LSKKEALRFVNR"
     gene            complement(31132..31500)
                     /locus_tag="PLF20_01170"
     CDS             complement(31132..31500)
                     /locus_tag="PLF20_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOX79653.1"
                     /translation="MMAEHNEMGKEGEELAAEYLVKQGYTIEARNWRYGHEEIDIIAR
                     KDDLLLIVEVKTRRSNYFGQPESFVSFAKQKALIRCANRYIYQHNFEGDTRFDIISII
                     MTPKGNQINHIPNAFYPTLR"
     gene            complement(31513..32424)
                     /locus_tag="PLF20_01175"
     CDS             complement(31513..32424)
                     /locus_tag="PLF20_01175"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOX79654.1"
                     /translation="MNHLYRRPAYLNSGDKICITSPAGRVAPEKVLKADKVFKNYGFE
                     VEIMSHALGAFYQFSGTDDERLEDLQQALDDPYCKAILMSRGGYGLHRIIDRLDFRSF
                     ETFPKWIIGFSDITLLHAHLHCLKWQSLHSPMASAFSIENPSQALKVLSFLQGNLPKY
                     SLAPHALNRKGKARGILTGGNLSILTSILGSCSDLDTNEKILFIEDVGEYLYRLDRMM
                     WTYKRAGKLSHLSGLIIGGLTEMKDGETNFGYSAEEIVYQAVKQYSYPVCFGFPAGHQ
                     PHNEPLMLGAEIRIEITDEGTEMIYLY"
     gene            32580..34652
                     /gene="metG"
                     /locus_tag="PLF20_01180"
     CDS             32580..34652
                     /gene="metG"
                     /locus_tag="PLF20_01180"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOX79655.1"
                     /translation="MFEIMASENNKSYKRTTITSALPYANGPVHIGHLAGVYIPADIY
                     ARYLRSKGEEVLFIGGSDEHGVPITIKARKEGISPQQVVDKYHEIIKKSFEELGISFD
                     IYSRTSLPIHHETASNFFKKLYDEGKFIEKVTEQYYDAEVGQFLADRYIIGTCPICGN
                     QGAYGDQCEQCGSSLNATDLIQPRSALSGSIPVLKATRHWFLPLNEYEAWLKKWILEE
                     HKDDWKINVYGQCKSWLDHGLQPRAVTRDLDWGVKVPIQGTEGKVLYVWFDAPIGYIS
                     ATKHWAQLTGGDWEKWWKSPDTRLIHFIGKDNIVFHCIIFPVMLKAEGSYILPDNVPA
                     NEFLNLEGDKISTSRNWAVWLDEYLKDFPGKQDVLRYVLCANAPETKDNDFTWKDFQT
                     RNNSELVAIFGNFVNRTAVLATKFFDGRVPELTSLLPIDEETMAEIAAIPARIASSLE
                     HFRFREALNEMMNLARLGNKYLTENEPWKIASSNMERVKTVLNISLQICANLAILCEP
                     FLPFTSTKLRRILNFNDGKWNDAGRCDLLPSSHILSTPELLFEKIEDEAIEKQIQKLV
                     KIRNTASETPKLEPAKDEIDFDTFSKMDIRVATILEAEKIAKTKKLLKLKVDTGLDKR
                     TIVSGIAEYYQPEEIIGTQVCILMNLAPRIIKGIESRGMILMAQDPQGKLYFVSPAEI
                     IPAGSQIQ"
     gene            complement(35033..35755)
                     /locus_tag="PLF20_01185"
     CDS             complement(35033..35755)
                     /locus_tag="PLF20_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79656.1"
                     /translation="MAVLQRERNMKTIFIIILVFFSINSFGQEWKNVKGKPKDLKESF
                     EYLDKMFDDTTKYTYMTLPSDVVFGKLYSFGLGMWIRNNWGLWGNSDLKKYFIENGIN
                     HPDIISGIILSEYYNYLNHIPYELKREVDSSLLQMTNKELVEKMESDMTKSNELLKYY
                     PIDDTIVVYVTVTKKKFLKTEKESVRAIAKVIKHENSELIVELLKIPIKKKQSTNYEA
                     GQKINVDPYWCELIPPKNWKWN"
     gene            complement(35916..36350)
                     /locus_tag="PLF20_01190"
     CDS             complement(35916..36350)
                     /locus_tag="PLF20_01190"
                     /inference="COORDINATES: protein motif:HMM:NF015629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0158 family protein"
                     /protein_id="HOX79657.1"
                     /translation="MTNKTTDIVKEIAEQLDCGFRAFIHKTSRQLLFVPDNNNYPDID
                     LDSWDEELEQLENNFTDYYEIDKWTSSEAFETMSEFADQLTDKNLQSRLFDALRKNKP
                     FKEFKLVIDNSGDFRQQWFDFKNKWQQDFVARQLNRLKPTDE"
     gene            complement(37237..>37447)
                     /locus_tag="PLF20_01195"
     CDS             complement(37237..>37447)
                     /locus_tag="PLF20_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79658.1"
                     /translation="LQVKINDGLNLLLLHRCLPQVAVTWLIEGLCSYSASVQVDSLVL
                     LNRHLRQAAKRCASCQENDNVNNKQ"
BASE COUNT        10673 a         6995 c         8004 g        11775 t
ORIGIN      
        1 ctttgggggt gcaaatttac atcttttttt aatatcacaa gtgctctatt aaaaattttt
       61 ttaattcccc tctcaaaagg ctaaaatgaa cgtctcagtt ttgagggtgc aaagttaaaa
      121 acattttaaa acaaaccaat gttcatttat aaaaaattta gaagatttct gttagttcta
      181 tggatttgaa caatttgaaa tcgaaaaaaa ttttactcgt tcaatcattt tttccttaaa
      241 tggctttaat atggattaca ttcgtagttt ttgtgatgtt atttgtttaa ttcatagttg
      301 gatacaactt atctcttact aagttcgttt tgaggaggag tggagaaatc attctattat
      361 gttcctgtaa gcttgtaatt acttgatttt gttttgagag gctgaagtta ggagggtaga
      421 attacttctt taaggtgttt attatgaaac gtttttcaag gtctcgagta tcaatttcca
      481 gaatttatca tccgacggga tatggatttt ttcatcgggt gaatggacat cacgcagggt
      541 aggcccaaag gaaaccatgt cgaggtaagg atattttttc aggaacaaac cacattcgag
      601 gccagcgtgt atagagcgga cccttggttc atttccgaag agatttttat aagattggat
      661 ggttgtgttc agaattctgc tcgaggggtt gggtgtccag ccaggatagc catcggaatg
      721 ggttacttta aatttagcta gtaggaaaac actttcgacc atggaagcga tgtcggtttt
      781 agcgctttcc agatcacttc tttggctcgt attaactgcc aatgtattag gccctgtaat
      841 ttttatggat gcaaggtttg tcgaggtttc aaccattccg ggcattcgtt ggctcatgct
      901 tattacaccg tgtgggcagg cataaattgc attgagcaaa cgctgttgcg actttttatt
      961 gatgatggct tcggggtggg ttgtgctttc tagggtcaag caaaaatggg gttcggtgta
     1021 tttgagttct tcgaccattt cggtatagaa ttctttaaag tactctaaaa gcatgttttc
     1081 ggttttgaag ggtaccagaa ttactgcttc tgcttctcgt gggatggcgt tacggagatt
     1141 tccaccatca atttgcgcca accggatatt atatcgattg ctggcattcc agaggaaacg
     1201 gttgagaatt ttattggcgt ttcctcttcc tttatggata tcgtcgcccg agtgccctcc
     1261 ttttagtcct gtaaccttta ttctataagg taaataatta acaggaacca ctcttctttt
     1321 gaattggagt tcggccagag tatctatacc tccagcacag ccaatgaata attctccttc
     1381 atcttctgaa tcgagattta taaggatgcg ccctttcaga aacccaggtt gtatggctga
     1441 tgctcctgtt aatcctgttt cttcatccac cgtaaacaga cattcgatag aaccatgcgg
     1501 aatatcatcc gattccaaaa ttgccagttg agcggccata cctattccat cgtcggctcc
     1561 caaggttgtg tttcttgctt ttacccattc accgtcgatg taaggttgta tggcgtctat
     1621 ttcaaaatta tgaagtgaat cttggttctt ttcgcatacc atatccatgt ggctttgaag
     1681 aattactatc ggtttatctt cgaaaccagg cttggcttct ttgcgaataa ccacgttccc
     1741 tgcatcatcg gtattggctt caaggtgatg aagtttagca aattccaaca agtagtttcg
     1801 tatctgagct tcgtttttcg atggcctggg aattttacaa atttcttcga aataatgcca
     1861 gacattttgt ggttgaagcg atgtcaatga ttgatccata agatatttat tatatttaga
     1921 gttaatgaga caaggtttat gattctaatg atttattatt tatgacacga gagcataact
     1981 tgtttgttga atgtttcaaa cactgatata tgggttttag tattaagacc gggtacattg
     2041 tgtcaggcga caaaataaag aaaaattttc gtgtatttct ttctcaactt tattcatgtc
     2101 aaggattcgt tttttaggaa cgaatttttg agatgaaggg aggtattttg ataaaacagg
     2161 tgtaataagt cggaatttgg atgaaatttc aagtgaagcc gcttttactg cacccttctg
     2221 atgtgtgttt actggatgaa ctatcaaaca aagaaatgct cattatttca gtatggtttt
     2281 atttaggttt gatcatcaat tcttcgattt cgtccggatc gataggaata tcctccataa
     2341 ggttataaga tggataaccg actacaaaag tattttcgag tcgaatgcca atgttttctt
     2401 ctggaatgta taaacccggt tcaaaagtaa ggatcatgcc aggggagaat acttcatcgc
     2461 gtgaacccac gtcgtgaaca tcgaggccta cgaaatggct tacaccgtgc atgaaaaatt
     2521 tggttcgcaa aggattttgg gggtcttgat gtaagaggtc atcttcgcta aagaggccaa
     2581 ggttgatcat ttctttttca gccaagtgat tgagttcgtc atttacctga ttgatggtat
     2641 tacccggtac gagcatgctt agtgcatgcc tttgcagtcg aagaacagcc tcataaacct
     2701 gaagctggcg tggagtaaat ttgccactga tgggaatagt gcgtgaacaa tcggatgcat
     2761 aatttttgta ttctgcacca aaatctaaga gcaataaatc ccctttttct aatttttgct
     2821 gatgtgaacg ataatgcaaa taacaggcat tggctccaga agcaacggtt ggttgaaacg
     2881 catgacaaca tccgtttcgg gcaaattcgt aagtgatttc cgcttcgatt tcatgttcga
     2941 acattccggg gctggtaatt tttagaattt ttctgaaaca gttaccggtt aattgggaag
     3001 ctcgtcggat gaattcaatt tcttcgggtt ccttattgag gcgtaatttc caaagcaaag
     3061 gagcaaggcg tgaaaaagta tgcaagggga acatttttcg cattttcaaa gcaaaacgat
     3121 gttcatttac ctttacctcg ggaaagaatt taggatattc gttcgaatcg atgaatacat
     3181 tggaggtcag aaccatgagg tcttttagag tggcttcgaa ctggtcggtg tagcgaatgt
     3241 ttgccatacc tgagatttgg cttgcctctt ccagacgtaa tttctgtcct tcccatatct
     3301 cggttgtttc gtcaggcctg agaataaaca atatttcttt cattttttca tcggcatgtg
     3361 aagggaataa aatcaaaatg gttttttcct gcccgattcc cgtgagataa aacaaatcgg
     3421 agttttgcct gtaaggaaaa tactgatcgc catttcgggg cataagatca ttactcgaaa
     3481 tacagactaa ggcgtgctga ggcaaaagat ttattaattt cttccgattc cgttggaata
     3541 aattgggata aacaatgttg gtgtcgttca ttgtaaaaat ttttaaaaga agggattata
     3601 catttattta ctcgatggat gaattcaaag gaaaatatta tttttgagat gcaaccttag
     3661 aagtatagca ttttgaaagc attcttgaaa tttttcggta gagaggattg ttttttccat
     3721 taaaatagtt tttaaatttg ttagttcaat catcgaattt attcaattat gaatatttat
     3781 catctcagcc gttaattatc atgcgaagat aaagaaaaaa gcctttaatt aatgtcttct
     3841 ataaaagaaa gggcaaatga ctgtattcga attaaataaa tattttatat tcactcaaac
     3901 cttataattt atgagtaaaa ctttacttag aatgacatct ttgtcgaaaa aggtttttgt
     3961 ttcgctggca gggcttttct tgattacttt tttgcttctg catctgagca tcaacctttt
     4021 tattttaccc attacaccca atcatgagga aacattcagg caggcggttc atttcatgac
     4081 tacttctccg ttgattaagg tgatggaagt ggtacttttt gcagctttta tcattcacat
     4141 attttttgcc gtaactctgc agatagagaa ctggattgca cgaccaaggg gatataaggt
     4201 ctggcaaaag tccagaactg attcaccttt ttctaaattg gccatatata cgggcatctt
     4261 cgttctcatc tttctgatca ttcatttcat gaacttttat tttgtgaagc agggttgggt
     4321 gtctccgccc gaaggcgcat atgaggtagc cgacaagcat gatttttaca atatggctct
     4381 caatcttttc aataatcctg tatattgcgt cttatacatc gttctgatca tcgtacttgg
     4441 gtttcatctt catcatgctt tccaggctgg ttttcaaagc cttggactga atcatcctat
     4501 ttatacgccc atcattaaaa tattaggttg gatttattcc atcttagttc cattgggctt
     4561 catcagcatt ccactttatt ttttgatttc gtaattcaaa tcattccatg atatgactca
     4621 actcaatgca aaaattccac aaggttcact tcaggagaaa tggacgaatt acaaggccaa
     4681 tcagaagctg gtaaatcctg ccaataaaag gaaatttgaa attatagtag ttggcactgg
     4741 attggccggt gcttctgctg cctctgcttt gggggaacta ggtttcaatg taaaagtgtt
     4801 ttgtatacaa gatagtccaa ggcgggcgca tagcatttca gcccagggtg gcataaatgc
     4861 tgccaaaaat tatcctaatg atggagatag tatctggagg ctgttttacg ataccatcaa
     4921 aggaggagac tttcgttcac gtgaagccaa tgtttatcgt ttggccgaag taagcaacaa
     4981 tatcatcgac cagtgtgtag cccaaggagt tccatttgcg agagattatg ctggatatct
     5041 tgataaccgg tcttttggag gcgcccaggt atcacgaacc ttttatgctc gtggacaaac
     5101 tgggcagcaa cttcttttag gagcttatgg agccttgagc aaggaaatca aacgcggttc
     5161 tgtcaaaatg tacacccggc acgaaatgct cgatctggtg attgtggatg gcatagcccg
     5221 tggaattatt acccgtgata cgattactgg caagattgag cgttttgctg cacacgcagt
     5281 aatcattgca actggtggct atggcaatgt ttatttttta tccaccaatg ctttgaattg
     5341 caatgcaagt gcaatatgga actgttacaa gaaaggggct ttctttgcca atccggcttt
     5401 tgttcaaatt catcctactt gtattcctgt gcatggtgat tatcagagta agcttacgct
     5461 catgagtgaa agtttgcgca atgatggacg aatttgggtt cccaaaaaga aggaagatgt
     5521 tgaagccatc cgcagtaaga aacttcgagc cgtggatatc cccgaaaatg atagggacta
     5581 ttttcttgaa cgtcgctatc ctgcctttgg taaccttgtg cctcgtgatg tagcttcacg
     5641 tgctgccaaa gaacgctgtg atgcgggtta tggcattgga cctactggtt tggctgttta
     5701 tctcgacttt tcggagtcca tacggcgctt gggtcgtgat gtaatcgaaa gtcgctatgg
     5761 aaatcttttc cagatgtatg aaaagattgt ggacgaaaat ccttacgaaa atccaatgat
     5821 gatcttcccg gcggtgcatt atactatggg aggcttatgg gttgactatg aattgcaatc
     5881 tactgttccg ggattgtttg tggcaggaga agcgaatttt agtgaccacg gagccaaccg
     5941 cttaggtgct tcggcattga tgcagggctt ggctgatggt tattttatcc ttccttacac
     6001 tcttcagaat tatctggccg atcagattgg cgttcaaaaa ttcgaaaccg atttgcccga
     6061 gtttgaatta gccgaaaaag aaacgattga aaagctgcaa cgtttgcttg ctattcaggg
     6121 aaaatactcg gtcgctacac tccataagaa attgggtaag attatgtggg aaaatgttgg
     6181 aatggctcgc gatgaggcta ggcttaagca ggccatagag gcaattcgct cattaaaaca
     6241 agaattctgg aaagaagtat atgtgcccgg ctcccttaca ggagtaaatg atgaactttt
     6301 gaaagccaat cgcttggccg attaccttga actcgcagag ctgatcgccc acgatgcctt
     6361 acttagaaat gaatcatgcg gatgccattt cagagtggaa tatcaatctg aagatggaga
     6421 agcgctccga aatgacgaag aatataaata tgtatcctgc tgggaatatc aaggagaagg
     6481 agttgaaccc caacttcata aggaacccct cgaatatgag tttgttgaat tgaaatcccg
     6541 taattataaa gtttaactca tttcctgaac ctaaaaattt aacattatga actttacatt
     6601 gaaaatctgg cgtcaggaaa attccaaagc caaaggaaga ttcaagacat ataaaattga
     6661 gaatatctca cccgattgtt cttttctcga gatgctcgat atcttgaacg aaaaattgat
     6721 ccatgataaa atagagcttc cggtcgatcc tgttgccttc gatcatgact gccgtgaagg
     6781 aatttgcgga atgtgcagcc tttatatcaa tggacatcct catggacctg aacagggcac
     6841 tacagtttgt caggttcaca tgcggaaatt ccaggatgga gacaccatcg taatagaacc
     6901 ctggcgagca aaggctttcc caataattaa agatttaatg gtagatcgaa gcgctttcga
     6961 taagattatt caagaaggag gttatatatc ggcttctact ggtggtgtgc ctgatgcaaa
     7021 ttccattctt gtttcgaaag aatatgccga tatggccatg gatgcggctg catgtattgg
     7081 gtgtggagca tgtgtggcag catgtaagaa tgcctcggcc atgttgtttg ttgccgcaaa
     7141 ggtttctcag tttgccctcc ttccacaagg ccatcctgaa gcaaaaagaa gagtgcaggc
     7201 aatggtctcc aaaatggatg aattaggatt cggaagctgt accaatactt atgcttgtga
     7261 agccgaatgc cccaagaaca tttcggtaga acatatcgct cgcatgaata gagaattttt
     7321 gaaatcgaaa cttactcatg ccgaaaagaa aagaattgga ggctttggct gataatgttt
     7381 ttttgattac tacccgtctt aaccaataca aggttgttga gaatagtttg ccaaaattag
     7441 aaggttcatt tttctttcgg caactatttt atttagggga ttttttgtta acctttattg
     7501 cttctgaaat tacttgaaaa aataggggga agataagtac gatcttccct tatgatagcc
     7561 tgtattgaaa atcaattggt tgtttataat tgtcaaatta atataattgt tcgaaaaatc
     7621 gtttcatttt ttatgaatcg aagaattata ctcaatgttt gaaactggat aaaagatcgg
     7681 aaatttgaaa tttatattaa ttctaaataa tgatgtgtag gctgataacc tgactgagag
     7741 tgaaactagg gtgataacca gggtaatgga tagaggtgct ttcaagcgac gaattatggg
     7801 aatggctttt atcgacgggc gggaagcaag caggcaagca agcaagggtt ttaaacgagc
     7861 tcccctgcga gaccctattg aatattccgt taagagtaat catgaatcga agaagcgcga
     7921 ttgaagtgag tctgatagaa atatcggagg atgggacaaa tgaaaaaaga atatctcggg
     7981 cttttgattt gaagaagagg gtaaaatttt tggagctaaa aaccccggca ttgccggggt
     8041 tggaaggggt tttaagcctt ttgctgttcc tgagggattc tatagaaact aacctattgt
     8101 ctatgaggaa attaatcatc gagtatcatc aacgacacat caggatggaa aggaacagcg
     8161 ctccaaatat aagacatttt ttaacacaat gcaagctttt tagaaaatat tttaaaatat
     8221 ttatttattg tatttatttt cagcgtatta aatttcgcga gtatcgagct ttcccttcct
     8281 tatttttgtt ttgggcaata aattttgatg tttaatagat tacgaaatta aatttcgaga
     8341 tttctgcaaa ccgcattata tatgggttat caaatgattt gaggggtggc atgcttttat
     8401 cttaacctcc gatggagtat gaattcgaga cgaaaggtga tcatagtgat aagaaaatgt
     8461 gtatttttgg agcttgagct gaaaagatcg aaattatgcg acaagtattg gtatttacag
     8521 tatatgtgct ttcattctta tggttaagct ggatggatgg aatagataat ttgaatgcac
     8581 aatcatacta tggtttccat cctcgaatgt gtttggccag ttatcccgaa ctaaaccatg
     8641 aaaattatta tccaccaggt cgagattcga agggggaaga tattgatgca aaccatgaag
     8701 atgcggagtg gtttgcaccg gaatatttct tccagtactc tatagacaag aacacaggga
     8761 aactaacggt aagttgtgct ttagaaaagg tagaagttga acacaataga gagagagggg
     8821 acattgatag ccaaagtgaa gattcatgca ggtgttcatc atccattgtg ataataccca
     8881 atcccgccaa cgatgaagtg attttgaaag gtgcctgtaa ttcatcggtg tatgttttcg
     8941 attttaaggg gagacagttg ttgttcagcc gtattgaaga tatacctcgc ctcgatatcc
     9001 gatcactcaa tacagggata tatagtataa aagttgagtg tgcagaagac atatatactt
     9061 tgcagctcat tattcagaga tagttttcaa gaatggggtt cgatttcctt attttcggga
     9121 tgtcgatgaa agaaatgcag tgagtcgccc cagttgtccc atccaatttc acagtgaatg
     9181 gcagagatac tttgaataag gtaattcaac gtttgttggg cttcttcatc tatattgggt
     9241 ttcccctcat ataagaggta gtcttcttta tattttaaag gtgtaagatt gggcataatt
     9301 acgttagctc ctactttcag cgccctgatt tttgcattgg gatcaatggt ttccatggcc
     9361 gtagtggcgg ctatgttgat gtcgggcatt atgatgcgca gcagtgcaac cattttgagg
     9421 gacagatcga gtctttcgag cggcgacaaa agcaaatctt tatattggta aagaggagtg
     9481 tccttgtgtt cgatatatgg tcccatgcct accatatcga tgtcgaggtt tttaaaaaat
     9541 tcgagatcga aagcgagatc atcataagtt tgaaagggaa ggccaatcat aacgcctgtg
     9601 cccacctgaa acccctcatt tcttaagtct tcgagtgcct ggagtcgttt ctcgaaggtg
     9661 tggaaggtgt cttggggatg catcttttga aataaatcgg ggttagaggt ttcgattcgc
     9721 agcaaatagc gatgtgcacc gcaatcgtgc caatagcgat agatttcgcg actttgttca
     9781 cccaatgaaa gcgtgatccc aatttttccc tgcgacagtt gtttaatttt tctgaccagt
     9841 tccccaattc gacacgtgtt ttggggtgat gataattctc ctgcttgaat aaccatagaa
     9901 gcaaagtgct ggtcgagaac aaattgcgct gcttcaagta cttcactttc actcaatttg
     9961 taacgcctaa catttttatt gcccgatctt accccgcagt aaaaacaatt cttggagcag
    10021 atgttggagt attcgaccaa acccctgaga tacactttgt taccgacatt gttctgctta
    10081 acttcagagg ctttctgaaa aattaaattt tgttcaccct tatcggccga taataagatt
    10141 accaattcat catgagaaaa agtttttttc cccaacaagt gtcgaatgct tggctttggg
    10201 ttcatgcatc atattttctg caaagatagt tcacaattct caggtggtta gatttgcgct
    10261 ttcgataatg attctttaga attgcaaaag aaaaattcgt ccaaagaatg aagtttcata
    10321 ataagttgtt attgaaagac gctgttatct tcaggaaaat tacaatgggg gataagctat
    10381 gaaaatattg agtgtagata aaatcaggga agccgatgcg tatactatag ccaatgagcc
    10441 cattgcaagt attgatctga tggagagggc ggcgcggcaa tgttcgcaat ggttgcttga
    10501 tcatttggta aaagatatgc ctgtgatgat tttttgcggt actggaaata atggaggtga
    10561 tggtttggct gtggcacgtc atctattcga aaatggtttc aaggtttcgg tttatattct
    10621 caggtttggc agccaggctt cgcctgattt tttaaccaat ctttgtcgtt tggagtcact
    10681 tcctgtggaa tgtatggata ttgcttccgc tggcgatttt ccggaaattc ctccgtttag
    10741 ttacattatc gatgccttat ttggcacagg tttgagcaga aagctggaag gtttggctgt
    10801 tgaattggta tcctacctca atcattcgag aggtattatt atttcgatag atataccttc
    10861 gggattattc gtagatactt cattgaaagc tgtttcgggg attgcagtgc aagccgacca
    10921 taccctgagt tttttaccac ccaagttgtg ctttatgatg ccagagaacg atatttatgt
    10981 tggagaatgg catgtgtttg atattggcct tcatcctgaa tatttacagc aagcggagac
    11041 gaagtatttc tattcttcga aggacgatgt tacttcatgg ttgaaacggc ggcggagatt
    11101 tcagcataaa ggagattttg gacatgcctt attgattgcg gggagtgaag gcaaagctgg
    11161 ggctgcaatt atggctgcaa gggcttgcgt gcattccgga gcaggattgt tgacggtgca
    11221 tgctcctggc aaggttggcg aggctttgca gataaacttg aatgaggcca tggtatcgtt
    11281 cgatgaacaa gcatcgttca ttacgcatct accttcgttc gatcgttatg atgctgtagg
    11341 tgccgggcca ggattgggtc tccaccgaca aacccaaact gcactcaaat tgctcataca
    11401 gaattatcga ggtccactga taatggatgc cgatgctttg aatattttat cggaaaacaa
    11461 aacatggtac tcatttttgc caccttatac catacttacc cctcatcccg gggaattcga
    11521 gcgactggct gggaaaaccg ataacgattt cgaacgtttg aaactggcta aggattttgc
    11581 actgaaatat aatatttttc ttgtactgaa aggcggtgta actattatag taaatccttt
    11641 ggggcaatgt tatttcaaca gtacgggaaa tcccggtatg gcaacgggag ggagcggaga
    11701 tgttttaacg ggaataattt taagtttatt ggctcaggga tataatcatg gacaagcatc
    11761 cgttatggga gtttttcttc atggttttgc gggtgatctg gcggccgaag agctttcgca
    11821 ggaatacatg tcggcggttg atattattcg gtttttggcc cctgcttttc aatctttatc
    11881 gaaaggacgg ggttgattag attttggatg cgagggtcgc gaaaatcatc gatgagaaaa
    11941 ttcgctccgg taaatctttc ttttgtaaat ggcgatttta ctactgctac catacaacct
    12001 gcgtttattg ccgttctata cctactcgag aatcttcaaa aacaaggcaa ttctggggtt
    12061 tttcccttaa acattctgcg actttcagaa atatttcggg atgaggttta ccatgagtaa
    12121 ccatagtggc atcgataata caggaaaagt attttcgaat gtcaagtgcg tcaatgacca
    12181 tgtcgacatt ggaagttgga gccgaagtag ccagagctgc agggatttga gcaatgaaaa
    12241 gattcttgag taaatcactc aaaccctcta cttcctttat ataaggctta tatagaccac
    12301 gatataaccc ttctttttct tcttcgaatt gcctgagttc tttttgagaa agtttgtgga
    12361 caaacacttt ctgtaagatg tcttcatttt taccaccaaa taccgctaaa agttcttctt
    12421 cagttattgc tatatggtga ttctgacaaa acaacttcca tgcattcaaa tgattttcat
    12481 gatttctaac gatagtgcca tccatatcga agataaaggc aagagggctg ctcatattat
    12541 tcatatttgc tgatgagagg ttgatgaggt ttgtaattga agaaattcaa gggagctata
    12601 cccgaaaatt cgataaaaac accacttcgg ttatctggaa gtccaggata aaatccaagt
    12661 tttagcagga tggttttgat gacgagattt tcgttgcgta ccattgcccc aattccaaaa
    12721 cccgaaaaga atttgttttt ttctggaaat tgttgtctac cgcaaagata ggccaaattg
    12781 acaaaatcga agaaagccag tcgaaagcca taaaaatagt atggagtgaa gagagtagat
    12841 gaataattga gggttaattt ttcattgccc gaataaaagg cagtatcgct tggctgagca
    12901 attaacagat caagttcttt caccacgttg actgattcgc cttcgagacg gttcattccc
    12961 tggcaataat tcaaagtgag aaaatgtcga agtttgaaag ggccgtaaat gtataggtta
    13021 gaaaagtatt caaggtgcgt tccccattga ccctgttcaa actttccgct acgattgtat
    13081 gacgaaatca agaatttacc cgaaaggtat cccaaacggt tgaaatattt tgccacgcct
    13141 gtggtgaatc caaagtaagt gcgattataa tattcatagc tttcggggcc cgttgtgagt
    13201 tcggcaagga aaccataggg aatgtcttcg gtacgtccga actggtatat ataattccct
    13261 ttataatagg cgtttctcga tatagctata ttgaggagta tatttgagga attatagtag
    13321 attttattag aatcggctga cacataaggt ctttgcgtat aaagtcggct atcaatactg
    13381 gagcttatta ccagttgggt aggctggcca tcgccatata cattgaatgc tttgccaatc
    13441 cataaagagg gattgaaata tttcaaacga tcctgtcgga aatagggggt gaagatattt
    13501 tggatttttt cgtgatgagt gagtgataag ccactggcaa aaggtgtttg agtggttaga
    13561 aaatctcgag caatggtcag acgaatgctt tcatgattaa attcattggt gtaatcggct
    13621 tcagagtaga agaaagttcc cttgatattt tcgattcgat aattgaggtt tttgagagaa
    13681 aagaccggag actttgccgt gttatagcgc actgttgatg aaagacgatt gccatacccc
    13741 aggaaattct ggtcgaaaag gctgaaatct acaagagttg agcttgggag ataaatatct
    13801 aatcccaaag ataaagcatc ctttgcccat aaaataacat ctactttttc ggggtttttt
    13861 tcatcggcca cggggcgtaa atatgcttcg gagaaatagg gcaaggaacg taataactga
    13921 gcattctcag ccattatata aggatcgagc ttttctcctt ctttaaacaa tagattgttt
    13981 cttattacgg tttttcgcgt ggttctatgt aaagaatttc ccatacgact caaacgcgcc
    14041 caggtaaggt tgggttgtgt ttcatcgaag ggcttatcca cataaatgat aattttttgt
    14101 attgtcctat cggcaaaagg caaatattca ttttcttcgg gcggttcgga gagggtttca
    14161 ttattttttg atacggctcc tgattctgga aacagaagtt gataagccag cttggtgagt
    14221 tcagtcttac ctgctttcga ttttaaagaa tcataaagta ttcgtgaacg tgcataaggg
    14281 tctttcaaga aataaactct tgagatgtca tctccaatga taatggtgtc ggtagaaatc
    14341 aataccaatt cgttgtcgag cagaattttt gtaccgcaac aaagccacaa agtatcgtca
    14401 cggatttgcg aacatggtat ttcctggcta aaaacttgct tcccaactat aaacgagata
    14461 actaaacata aaaatgatat ggatttcttt tgcaacacat catcatgaat tacagagcaa
    14521 acgccagctt tgatttttta tgatacgaaa aaagaggata ataaaacgat gcattacgca
    14581 agacccatat catgcttgat gtcttcgatt ttttggctta gaaactggtt cgtcttttca
    14641 taatcttcaa tatggtgaag aatggttttg gctccaaaat agaatttaat cttaggttcg
    14701 gtccccgaag gtcttacggt gattttgctg ccatcttcga ggaaaaattg aagcacatcc
    14761 gatttgggca agtcaatgtg gattttttcg cctgtaatca ggtttatctc tgtttggcat
    14821 agataatctt ttattactgt aaccttgata ccggcgagat gttcaggaag atttgccctg
    14881 taattttcca tcatttgacg gatttcttcg gctccggcaa ttcctttatt agtaatagaa
    14941 agaagggttt ccttataaaa tccatattcg agatagattt ccttgagaag gcgatagagt
    15001 gatttaccct gattagcagc ccaggcagct gtttcggcta tcatgcagca ggtgcttatg
    15061 gcatctttat ctctgacaaa atcgcccgta aggaaaccat aactttcttc tcctccggca
    15121 atgaaatgca tggaaccttc caaacgtctg attaaatcgg caatatactt aaaacctgta
    15181 agtacatcga agcatttcac tccttccttt tgggcgattt cctttataag ttcggaggtt
    15241 acgatggttt ttacgatgaa ttcgtttccc ttaaggcgtc ttttggcttt ccatgctttc
    15301 aaaatgtaat agataagcag agaagcagtt tgatttccat tgagaagcac gaattcacct
    15361 ttttcgtctt tcacggcgat gcccacgcgg tctccatcgg ggtcggttcc catcacgagg
    15421 tcggcaccgg tttcccgggc ttttttgatg gcaagatcga gggctgcggg ttcttcagga
    15481 ttggggctat gtacggttgg aaaatctcct gataccatca tttgttcatc tacggtaaaa
    15541 atttgttcga aaccaaattc ttgcaacgcc catggagcaa gtctcactcc tgtgccatga
    15601 ataggagtat agactatttt gagatttttg taccgggcaa tgatctctgg atagagtgaa
    15661 agatttttta tagcggcgat ataagccctg tcaaccgctt ctcctatggt ttcaatcaat
    15721 cgagagttgc cctcgaattt cacttcttct attcccttta tcttgctaac ttctgccatg
    15781 acattcttgt cgtgggggct cacaagctgg ccgccgtctt cccaataagc cttgtatcca
    15841 ttatattctt taggattgtg tgaagcagta ataactacgc cactctggca tccaagaaat
    15901 ctcacagcaa acgaaagctc gggtgtgggc cttaaatcgt cgaaaaggaa aactttgatt
    15961 ccattggccg ataatacgtt tgccgtagtt tgtgcaaaaa ggcgtccgtt gttgcggcaa
    16021 tcatgagcta tgactacttt tatgggattc ttatcgggga agttcatttt taaataattt
    16081 gccaagcctt gtgtggccat tcctaccgta tagatattca ttcggttagt cccgggtccc
    16141 ataatacctc gcagtccacc tgtaccaaat tccaggtcgg tataaaaact ttcgatgagt
    16201 tctgctggat cgttatctaa aagatattga accttttggc gcgtttgttc gtcaaaaggc
    16261 ggttgtgtcc agtgatgggc tttttcgaga atagctggat ttatcttgtt catggttacc
    16321 gtattttttg aggataagta attttattaa gaatgaatcg tacaggttta acctgcaaag
    16381 ttattaacaa tccaataggt ttgcccgagt ggcggagtac ggccatcatc tttctgtgtg
    16441 cttcgcttga ttccatttca atttctacag gtatttcatt ttctatcaaa agatcgacct
    16501 ttagttggtt ttctggcttg aaatatttgt aggttactgg gatgaccaca ttttcacgga
    16561 atgataacat tgctgagcgc aattcaaaaa tcaagcattc cctgtaagtg gcaagaggaa
    16621 ggccttctcc caatttttcc catatttgat tcatcatgct ctcgatttga gcaatgatac
    16681 gatttcgatc ggtattattc attcagcaat tgtatacatt ttttcaaact ggcgttccaa
    16741 tgcggaattt cgatattcag gcgttgacgt gtattcgttt tatcgagaac gctgtaaaat
    16801 ggtcgtggag ctggtaaggg atattgttcg gatgatatcg gatgaattgt acaactgata
    16861 tctgaatgtt ctacaatgct gatggcaaag tcgtaccaac tgcacacccc ctcattggta
    16921 taatgcagaa tttcaactcc ttcgggttct atccgatgaa taataacatg gaggatcatt
    16981 tcggccagat cggctgccca agtaggcgaa ccaacctggt cggagactac atcgaggttt
    17041 tgtttttgta aagcattgcg gcgaatggtt tttacaaaat tgcttccgaa actcgaatac
    17101 aaccaagctg tacgaatgat gaatgcattg actcggcttc tcatgatggc aatctcaccc
    17161 ccttcctttg atttcccata ccacgacaaa ggatgagtgg ggtgagaagg gaggtaaggt
    17221 ctataatttt gcccatcaaa aacatagtca ctggaaatgt gaataaggca agtaccggtt
    17281 ttacgtgctg aattggcgag tatttctgga gctatggcat tgattttcca agcggattct
    17341 ttttcacttt cggctttatc gactgctgta taagctgcac aattgatgag atattgaggc
    17401 tggaggcttt ccattatttc atttactgcc tcgggttggg taatatccaa ttcggtaaca
    17461 tcggtaaaca caaaagataa atgattcaga tgtgcactca aaagcctcag ctcatttcca
    17521 agctgaccat tgctacctgt tacgagtatt ttggtcttca tttcaataat tttgtttgct
    17581 tttcttctat tattttatcc agtttcagcg tataagcttg cggaaatatt ctatagtcat
    17641 ttggaggcca tcttcaagtg cgactttggg ttcccaattc aatagatttt tagccagggt
    17701 aatatcgggt tggcgttgtt tgggatcatc gggcggaaga ggttgaaata taatctttga
    17761 gcggctacca gtgagccgaa tgactttttc agctagctcg agaatggtaa attcatctgg
    17821 atttccaatg ttaacaggac cggttacatc gtcaggcgta tccatcattc taatggctcc
    17881 ttcgatcaga tcgtctacgt actgaaaaga ccgtgtttga gaaccatcac catagatggt
    17941 gatattttga ttttttaatg cttgaacgat gaaatttgaa accaccctgc catcgttggg
    18001 gtgcattttt ggtccataag tattaaagat gcggataatt tttatgcgaa cgttgttctg
    18061 acggtagtaa ttcataaata aagcttcggc gcatcttttc ccttcgtcgt aacatgctct
    18121 tggccctatg ggatttacct ttccccaata atcttctgtt tgaggatgaa catcgggatc
    18181 gccataaact tcgctggtgg aagcctgaag aattttgaca cggattcgct tggccaagcc
    18241 tagcatgttg atagccccca ttacggaagt ctttattgtt ttaatgggat tgtactgata
    18301 atggatagga gaagcagggc atgccagatt ataaatttca tctgcttcga taaaaaaggg
    18361 catggttacg tcgtgcctga tcagctcgaa ataggggttc gacattaact ctactacgtt
    18421 ttccttggag cccgtaaaat aattatcgag gcaaattact tcatggccta aatctaaaag
    18481 acgtttacag aggtgagagc cgataaatcc cgctccccct gttaccagta ttttttttct
    18541 catgagatat aaattcttga agggttatgt tttatgtaat tagaaggtgt ttatgagcaa
    18601 tatgatgttt ttcattttag tgaaggaatg ccaattccaa agtaatcaaa gcccatgtct
    18661 ttcatttcct gtttggaata aatgtttctt ccgtcgaaaa caacagggtg gcgcatcaat
    18721 tttttcacca cattgaaatt ggggaatttg aattcgggcc attcggtgac gagcaagaga
    18781 gcgtcagcat caatggtagc ctcgtactga tcttttgaat attcgatttg atcgccgaaa
    18841 taacactgtc tggcttcatt catagccaca ggatcgtaag ctttcacttt tgcaccaacg
    18901 gagagcaatt gatgaatgag tgagatagaa ggtgcttcgc gcatgtcgtc ggtgttgggt
    18961 ttgaagctca atccccagat agcgatggtt ttgtcattaa gttggccgct gtagtaagaa
    19021 tttagctttt tgtataaaac caatttctga ttttcattaa cttcttccac tgccttcaaa
    19081 acgcttaatt gataaccttt ctcggcagca gttcgtataa gggcttttac gtctttggga
    19141 aaacaacttc ctccataacc aataccagga tagatgaatt tagtgcctat gcggctgtcg
    19201 ctaccaatac ctttgcgtac catatttaca tcagctccta ccaattcaca aagattcgca
    19261 atgtcgttca taaaactaat cttggtggca agcattgaat tggccgcata cttcgtcatc
    19321 tcggccgaag ggatatccat aaaaatgatg ggatgcccat tcagcgtaaa gggtttgtaa
    19381 agtcgctcga agatatcctg agctctttcc gaatcgatgc ctaccacaat gcgatcgggt
    19441 ttgagaaaat cgttgatggc atcgccttct ttcaggaatt cgggattgga agctacatcg
    19501 aatggaatgt ttactcctct tttgtcgagt tcctcctgaa tgacatgcct gactttttcg
    19561 gatgttccaa ctggtacggt actttttgta actacaagca aataatcttg catataacgt
    19621 ccaatttcgc gagctacgtt gataacatat tgcaaatcag cacttccgtc tccactaggt
    19681 ggtgttccta cggctgtaaa aaccatatct gtaccttgag atatcaccga tttcaaatcg
    19741 gtcgaaaaaa atagccttcc tttatcaata tttcgcatga cgataggctc gagcccgggc
    19801 tcataaatgg ggataattcc cttccttaaa ttttctattt tgcgagaatc tatatctaca
    19861 catgttactt caattcctac ctcggcaaag caagtaccgg taacaagccc tacatatcca
    19921 cttccaacaa tcgtaatttt catagaataa tgattttatg taatgtatct tttcttcgac
    19981 aaatttatga agagacatca attattcaat atagtgagga taaatctggg taaatacgaa
    20041 ttgattatat tttaaccaaa tagttaaaat tcactgaata actttggttt tagaaattgg
    20101 tttaaaatta atttatggtt ggttaatgta tcaaattttg ctttacgatg cagatgacac
    20161 gtacgtttga tttactcgat caatggatag aaaaatatcc aaggccagat gtttttggtg
    20221 ccaagagaaa tggtagatgg atacattttt cggcagtaga ttataaagaa ttttcggaga
    20281 gtttgtgtat gggactaatg gcacttggat tcgtgcaagg agataaaatt gctaccatta
    20341 cagcaaatcg gcctgaatgg aattttgtgg atatgggcct tgcacttggc ggatttgtgc
    20401 atgttcccat ttatccaact ctcaatgttg aagagtatta ttatattctc gaacattcgg
    20461 atacaaagct ggtttttgta ggcgataaat cactctatgc caagataaaa cccattgccg
    20521 atgctttgcc acaaattcaa atggtggtta ctttcaatga agtggaaggt gcgcctcatt
    20581 ggatggaaat tgtagaaaca ggaaggaaaa tgagagaaaa atatgatggg ccattgaaga
    20641 aaataaaaaa cagtataaag cccgatgatt tagttactat tatttacaca tcgggtacga
    20701 cgggcaagcc caaaggcgtc atgctgagcc attggaacat tatgtcgaat accatagcca
    20761 cttctaagat caatcccttg acgcacgagg acaaggtttt gagttttctt cctctttcgc
    20821 atatctatga aaggatgatg aattatgatt ttcaatattt gggcataggg atttattatg
    20881 cagagaatat gggcagtatt gtggcgaatt taaatgaaat tcaggccaat gagttcaatg
    20941 ctgttccgcg attgctcgag aatgtttacg acaggattgt agctcagggg aaagatttaa
    21001 atggcatcaa aaaggcaata tttttttggg cattgcatct ggccgagcgt tttgagttta
    21061 acaatgccaa tggatggttt tatgatttgc agttaaaatg ggctgataaa ctggtatttg
    21121 tgaaatggag agaagcctta ggcggccata tcaaggtaat agtttcagga ggttcgtcgt
    21181 tgcaggaaag gttatgtaga attttttggg gtgcgggttt gcccgtagta gaaggatatg
    21241 gattgacaga aacttcaccg gtaattgcag taggtcattt taactggcca gatattaaat
    21301 ttggtacggt tggacccgta ttggaaggcg tggaagtaaa gattgccaat gatggtgaga
    21361 ttctcgtgaa aggacctaat gtaatgctgg gttattataa ggatccagaa tatacagccc
    21421 aggttatcga tgaagatggt ttttttcata cgggagatat tggcgtgctg gtggatggta
    21481 aatttctgaa aattaccgat cgcaaaaagg aaatcttcaa gctctcggct ggaaagtata
    21541 ttgctcccca ggtaatcgaa aacaaattca aggaatccat cttcatagag gaaattatga
    21601 ttattggtga aaacgagaag tttgtgagtg ccttcatagt tcctaatttg aattatttgc
    21661 atttttgggc ttccaagcat aaaattcatt atcacgacaa tcgagagctt ataaagatgg
    21721 aagaagtaat cgagcgaata gacgaagagg ttcagagact caacaaaaca ttgagccttc
    21781 acgagcaggt aaaacgattc agactcgtgg ccgatgaatg gtcaccacaa acaggcgaac
    21841 agagccctac attgaaattg agacgtgagg tcatttatgc caaatattca tctttatatg
    21901 aagaaatata tggtcatcca cgcctatcga aaaataatgg caagaatagc gaaccgcaca
    21961 agccttccaa atatgccttc aatttgaatt tagggcttaa attcaaaccg aaggacaaga
    22021 gcgaagaatg aatgaatttt gaaatcaacg aactataatt tgttagagct caaaaacctt
    22081 cgtattgata ggctctggag caattatcga gcgaccaaca tttttttatg ggttttacga
    22141 taaaagcaat ctcccatgcg ttttgatgcc agggtatcat attctgttga ttttttagag
    22201 caaagtctct gctcatttca agatcgagcg atagggtgct ggttttaggt gtgtagattc
    22261 tgaatcccgc cgttaaagat agatgatatg tattactgta atatataccc atgcggaatc
    22321 tatcggcaaa agcaaaatgc ctacggtaaa tcaggctgaa gccaccattg taaagatgct
    22381 gcccttcata tatccatgaa gttctgaaaa ccataccgag tttctggctc aagggtaaaa
    22441 gatatcctgc atgtgaatga atttccttac cgggatgaat gtcgggtatg agagtacgat
    22501 aaggcatcga caatctgtaa atggccattc caatctcgaa gcgatgcacc tgatcgtcgg
    22561 tccaacagtc cattttattg attcctaata aaatacctac attcactcca aattttttat
    22621 gtggagaatg gcctggcagc aaagtgtcgt tgctttgaaa ataaattttc gaaaccggat
    22681 agtaaccctc cgattcgaac cagtagggct gaagaccaac tgcaagtgtg ttggtacctc
    22741 caaatccggc tagaatatcg aaacttgcgc cgataatagc gtatggatac tgagtggtat
    22801 cggcaaaacg tcgttcgctg aatatgccta tgccaatgga taagggattg gagcgaggca
    22861 acaccaaacc atactcctta ttagtgtttt cgaaattgaa gcgataagaa gcagataaat
    22921 ataaagtgtt caaattcgga gcattttcat aggaacgagt ttgataaact ccatccagtc
    22981 gatatttttc tataccaaat gctgttaagg ctgggttttc ctgctgaggg gcattccacc
    23041 attgcgtata tgctgccggt tgggaactca aatagctttt tgcttccaaa ataaaaataa
    23101 gagccaataa aatagtcctt cgtttcatgc ggcatttaac gaaatcaacc attatagatt
    23161 tatgattcat gattcgaaaa ctatgttatt acgtgatatt ttatgtgctt ataattttag
    23221 ttggttggga tatttatatc aatgactcat ctaaattctt ttgttaagat gaatttcaaa
    23281 gccaaattca agtagaatgc atgcggaaag atcagaagaa acttagttgc tgtccctcgt
    23341 gtttttcggg cagattggat tttgtcctga tggcattggc tggcgaaatg gctgcaagaa
    23401 aatggctagg gggaagtttc atgtttcggt ggaaaatctt gcgtgagtcg gcatagttta
    23461 aggtaagtgt ttttttggca cgagtcattc ctacgaaaag caggcgtttt tcttcttcag
    23521 ggtcagtttc ctgattttcg aaaagggaat agggaataag gccttcttcg caaccgctga
    23581 taaaaacatg ttcaaattca aggcctttag ctgcatggag cgtcataagc tgaactgcat
    23641 tttctttggc ttcataaagt cctgtgggag cattcaaatc gatgaagtct atgaaacttt
    23701 tcaaatcatt cccgaaatct ttggcaattt ccatgaaaat attccggtta tcaatggtgt
    23761 cgtttttttc tgagagattt tgttcgaaat aggctttcag aagattttca actgtgcatt
    23821 tattttcctc agggttttcg aggattttac gcaagtagag tatgatttcc gagagcaatt
    23881 ccgaatcgag caaatttcct gcttgagcgg tttgataggg aaggccatga ttgttcaaag
    23941 cagcgatgat ggccgggaac tgtcgcgttg tacggcataa gatggtgaat tcagataatg
    24001 ggatttcagt ttgagcttgc gctacaccgc tgtcgtaagc gaaaaaacgc gtgcctcccg
    24061 tcatggcatc tatagatcga gcaataaatt cggcttcact tttgtcggta gcatgttgca
    24121 caatgttcac tttgcctttc tcgggcaagc cttcgagggg attttcactg ccaatgactt
    24181 cataggcagc cttcaagatt ttatctccgc aacgatagct tttccccatg tggtaagtag
    24241 tcgcttgggg aaaatcctga agaaaagttt cgaaaatacc cgaatatgct cctctaaaac
    24301 cataaatact ttgaaaagga tctccaatgg caaaaacatt attttcttgt ggatgactga
    24361 tgatctttag aaattcgtat tgagctggat ttacatcctg aaattcatct attaaaataa
    24421 acccattacg attttggatc tgcgaacgga attcggcatc ttcttgcaag aaattcaaag
    24481 gcaataagat aagatcttcg tagtccatca attgattgtt gagaagatag aattgatatt
    24541 gattggcgat tttctgaaat gttacatcaa accgaaagtt aagggcatga tttttaagga
    24601 gggaaatgta gttgacgatt ttaaccgggc tgtaattttg gacctgagga agtgattgaa
    24661 tgatttttat tttttcggtt tcgctgatta gtttgaagga agaataagag gggatttcgc
    24721 ggagaatttg caagcccaag gcatgaatcg tactgatgga gagagatgat gcttgttgat
    24781 ggagtgaagg atgggacata aggcgttggc gcatttcctg agctgcccgt gtggtaaagg
    24841 taatggcaag aattttttcg gaaggtgcaa tattttgtga tatcaggcga gcgattcttt
    24901 cggtgagtac gtatgttttt ccgcttcccg ggcctgctat ggcaatggct ggtccttcgt
    24961 cgtgtgcgat agcagctttt tgcaagtcat ccgtggtagg ataaactgta tcctgttcgc
    25021 ttgaggaatc tggaattgcc gaataacgta ttttttgttc gagcacatga tggatgttga
    25081 aactgagcag gggataggct gttggacgat ttaatgggtt ttcatcgtga aatagtgaag
    25141 atttttgggc atagctttgt gcttcgcctg ggcgaaacac tttaatggtg ccatattccc
    25201 cgtcatatcc tggagtaatc attacccggc gctggcgcaa cctttcgatc gcttcggcca
    25261 gctcaccacc agcgtgttcg gcaatctgtg agcaaggtat ctctaaaagg acttttagct
    25321 ccggacctaa gatttttaaa atttgaaaat atttctccat taccttcttc gacgcagatt
    25381 ttgtgtgaat aatttctgca ataatttcag gcagaggaat aatataatga aaacggggtg
    25441 aaatagattc ttcatcgggg agagttttcg aagctaattg aaacacccta tcgagcacgc
    25501 ccgaagtgag ttttttgtag caaaccggac aaattccctg atgataagct gcttcatcgg
    25561 gtggtaatga aacattgcat tttctatgac ctgcaaaatg atatttccct tcctgcggga
    25621 aaagatcgat ggtttcggat cgatgactgg ccatagcctc aataatagcg tcgaaggatt
    25681 tatcgcagaa gaagatgtta gcatttcggc caagccggtc gggagaatgg gcgtcggaat
    25741 tggaaatcag gtgaaaccga tctaacttgc ctaccacacg gttcatgggt acatcactcg
    25801 aaaggccagt ttcgagggta tggatatgtg gggttagatc ttcgaaacat tcttctatac
    25861 tgtcgaagcc tgaactggca ccaagaaccg aaaaccatgg cgtccagata tgagcaggta
    25921 ccaatacaca ctgtggatta atatccaaca ctaaacgaaa caattcataa gaatccattc
    25981 ctaaaatggg tcttccatcc gaagtaatgt taaagctcaa gagttttagt ttttgctgta
    26041 tgaggcttgc ggtacggaaa tcgggaatca tcagtacatt gtgtactttt cttaccttgc
    26101 ctcccttttt atagatgttg ctgatttcgg tagtcagaag aaatagaggt tcctgagact
    26161 cggggatgtt ttcttcggcc aagcggtaaa ttgtttttaa tcgatataat cccgtttcgg
    26221 caggttcgag tttttcctgg agttccgaaa gccagttagg atgtatgcaa tcgcctgtcc
    26281 ctattacatc tatgcccttt cgcctagccc aaagatgaag atactccggc gtaagttcct
    26341 tactggtggc aagactgaaa tgagaatgaa tatgcaagtc agcaataaat gacatcggaa
    26401 tggtattaaa attattgttg cgaagttaag agaaaacaaa agggagagga attcttgaat
    26461 tctcaacttg ccaacatcaa attggaaagg atgggttatt tgattgggag cgaatgttca
    26521 gaaaaaccat tgaatgagaa tcttcaaaaa atacagcttc aacttggatt ctgcctttgg
    26581 tgattatcga agccatacct ataaattgca gatttatgtc taaatatgga tcccggctgt
    26641 ttcgttagaa gaacattttg ctttgtagga ggaaatttag tccaacaacg atttagccaa
    26701 gcagtggagg tttatttttt gttcttttcg atggcttttt taatcacttc ttcctgtaaa
    26761 gtttgcggca gtggcatgta ttgaaagaat tccatggaat aattggctct tcccgaactg
    26821 atatttcgaa gttgagtagc atagccgaac atttccatca gaggtactag gccattaatt
    26881 ttttgtgatc cttttcgatg ccttcgcatc gattcgatct gtccgcggcg tttatttagg
    26941 tcggtgatta cattgccgat atagtcatcg ggtgaattga tttcgatttt cattacgggt
    27001 tctttcaaaa tgggagctgc tttttgaaaa gcctgtttaa aacatataga agcacaggtt
    27061 tgaaaagcaa agtcggaaga atcgactggg tgatagcttc catcgaggag ggtaacttta
    27121 acatccacaa cggggtaacc ggcaattacg cccttttcga gggttttgcg aattcctttg
    27181 tccactgaag gaataaattc gagaggtaca atcccgccct tgattttatc gacaaattca
    27241 tatccttttc ctgggttggg ttcgatacga agcaatacat gggcatactg gcctttgccg
    27301 ccggtttgtt tcacatgttt gtaagcctgt tccgtttcgt gtgtaatggt ttctcgatac
    27361 gctaccgaag gctcatccgt ttgcagttct aatccaaatt catctttaag ccgatcgaga
    27421 atgatttcga gttgaagttc gcccatgcca gatataatag tttcctgagt ttcgtcgtca
    27481 aatcggacaa tgaatgaagg gtcttcgctg gccaatttat gaagtgcttc acccattttc
    27541 tgctgatcgc tttgtttcga aggatttact ttcaattcga tcactggagg gggaatgaaa
    27601 atggattcga gcaacaaagg attgtcaggg tcgcagaggg tatcgccggt tttagtaaac
    27661 ttaagcccga ccaatgcaac aatatcaccc ggcccagctt caggaatgtc ttctcgcatc
    27721 ttggcatgaa ttcgcataat ccgtcctatt cgctcgtttt tatcttttga actatttagc
    27781 acctgcatgc ctgtactcaa tctcccacta aaaattctga taaaagtctg ctgccctaca
    27841 taatgatcgt aaataatctt gaaggcaagt gctgagaaag gatctttagg agaagggtag
    27901 cgcgagtgtg ttttttcggg atctgttcga tccataccca caacagcacc tacatcgaga
    27961 ggactgggca agaaatgaat gactgcatcc agcaattgtg taatcccctt gtttttatag
    28021 gcagctccac aaagtacggg tgtagctaag aggtgcaaag ttgtgtaacg actagcctct
    28081 ataattaatt cttcagggat gggttcctcg ttgagatatt tctccaaaat ttcttcattg
    28141 aaatcggcga gtttttctat catatattgt ctggctcggc tggccgatcc cttgtattca
    28201 gcaggtattt cggcttctgt gctttcgaaa ttctcaaaaa aaagagcctt catctttatt
    28261 aaatcgatga ttcccttaaa atcttcttca ttcccaatag gaatctggaa aggaataggg
    28321 ttggcatcaa ggtatttatt catttgtgcc actacatttt cgaaatcggc tccctgcctg
    28381 tccattttat tgatgaaagc gattcgtggt actttatagc ggttagcttg attccaaact
    28441 gtttcacttt gtggctctac cccacctacg gcgcaaaaaa ccgcaatcat accatccaaa
    28501 acacgcagag atcgttcaac ttcaaccgta aaatcgacat gtccgggtgt atctatcaga
    28561 ttgatatgat gttctttcca tttacaggtg atggctgccg aagctatggt tatccctctt
    28621 tcctgttcct gcttcatgaa atcggtagtg gcctctccat tgtgcacctc gcccatcttt
    28681 cgatttgcac ctgtaaaata taaaattctt tcggtaactg tggtttttcc tgcatctata
    28741 tgggctgcaa tgccaatgtt tcttaattta tgtaattgca taaaaatgat taatcttgaa
    28801 ataattaatt aaaatacaat attttatatt ttaattcgat aaaagggggc aaatctacaa
    28861 acattctatc agaaaatgga aaatcagcaa aaattctcat tggttgaatt ttgtatcatt
    28921 acgtaatcat gagaacaaca agcaaaggag ggtggggaat gatgagtgga aagtcaaaag
    28981 ttgaaagtga aacatgatga gtgcggtgtt tgtatctgaa tagggttgga tagtgcacat
    29041 ccaggagaaa ttgtcaattt ccgttctgaa tcttattaga tgagaaaggt taatagagcg
    29101 agggtgagta cgaagcgaag ccacacaaga ttctaaaatc taggatcgat attcgagaag
    29161 catatttttg ttcattaatt tttatttttt cattgtctca atgtttcttc gaattcctaa
    29221 actatattcc ttgagatacg aaccatcaca acctgatagc tacgcctttg atgccttaac
    29281 aaatcattct tagtaattag ttcgataata atttcaagga gttgcaggga cggaatcatt
    29341 gagttcttca aataaggtgg tatcgggttc atttgaaaga taagcaccca ggtacaagtt
    29401 cctgaaatga tgatcggctt ttacgaattc ccgttcggct tgagtaagtt ttttatatat
    29461 gtttttcctc aatcgcatga tacgagcatc atcagcatcc gaaatctcgc ctggtttttt
    29521 aacaatagca atcatttcct gaatttcatt ttccaatact tcacaataaa ccttcaaaag
    29581 ggaaagagtt gcctttaaaa gaattgtatc gccctgataa tcaccaattt tttctatttc
    29641 ctgtagagaa tttttcgcat aatcgagagt tttattatag gctcgttgga ggccggcagt
    29701 atcggcgtgt tcgagataac ggttgagatc gaaaatggca tcggccaaag tgttttgttt
    29761 ttctaatatt tgatcataat attctttttc atcgggcgat gatggagttt tacatgccgc
    29821 aatacttatc attatgagca agcaaaatag gtgggcagtt ctatccatag accatatcta
    29881 taattttttc gagccatttg gcatcggttt cgttaaagtt gtcaataagc tggctatcaa
    29941 tgtccaatac tcccttgatg tttccccctg catctttaag ggggacagca atttcggatc
    30001 tcgaacggct atcacaggca atatgaccgg gaaattgctc tacgtcaggt acaatgatag
    30061 ttttttgctg ctggatggca gcccagcata ctcccttgtt tttttcgagt atctgacatg
    30121 ccagtggccc ctgataacac catacaatca attcatcatc tacaagatga taaaatcctg
    30181 tccagaaaaa attcggcatc ttatgatgca gcacagcaat tattgtgctc attcgggctt
    30241 cgggcacctt cgtcttggtc aataaagttt ctaattgacc atagatgtgt tgataacgta
    30301 tttcttgatt ctgaattttc atcacaaatc aaataatgat ttcaattaca atagtttaac
    30361 ttttaaactt catcactggc ttccaaaatt accgaaaatt tggaaatctc tcaaaaattt
    30421 atattatgaa tttattcgaa attcatcgat ttacaaatcg taaggcttct tttttgctta
    30481 aaggctttaa gggttggtga gctacaaaat gcatcacttc ttcgggaaat gttttcgaca
    30541 cttggcgtaa tgcccatcct atagccttct gaataaaaaa gtcatccgaa tcacttaaac
    30601 actgaatgga tcgaaacaat aaatcaagat gggttttttc tttgtaattt aattgaaaaa
    30661 tcagtgcagc tcgctgtagc catagatttt gactatcaat ccattttgaa gttgtatttt
    30721 cgatgatatc cggaaaatga ataaaaaatt ttccacaaag tttaggactg ataaaatcta
    30781 ctgtatccca ccatgccttg ttcgtcatca tccattcgag cagtacaata tgttcttcgg
    30841 tgcaatgagg aataaaatgt tgagaatact ccattgcaaa atattgcatt tcacgatagg
    30901 cagagttcca acactgaaat atgagttcag gccattgctc gatggagaga tatccatttt
    30961 gtagaaggaa gcctttttga agccgacgac gcaggggtga aggaagtccg taaaactcga
    31021 actgatttcg catataagct ttggcgccgc ttgccgtttc tctattgccc tcctcttcga
    31081 atatggatga tagagccgaa acataagaat gaatgtcttg agtcggaatc atcatcttaa
    31141 ggttggataa aaggcattgg gaatatgatt gatttgattt cccttgggtg tcataatgat
    31201 ggaaataata tcgaaacggg tatcaccttc gaagttgtgt tgataaatat aacggtttgc
    31261 acagcgtatg agggcttttt gcttggcaaa actcacaaaa ctctcgggtt gtccaaaata
    31321 attggaacgt ctcgtcttaa cttcgacgat gagcaaaagg tcgtccttgc gggcaataat
    31381 gtcgatttct tcgtgcccat atcgccaatt acgagcttct atggtataac cttgtttcac
    31441 cagatattcg gcagccagct cttcgccttc ttttcccatt tcgttatgtt cggccatcat
    31501 tgtaagattt ttttaataca aataaatcat ttcagtacct tcatccgtga tttctattct
    31561 aatttccgca cccagcataa gaggttcatt gtgtggttga tgcccagccg gaaatccaaa
    31621 acaaaccgga taggaatatt gctttacggc ttgataaacg atttcttcgg cactataacc
    31681 aaaattggtc tctccatcct tcatttctgt caggcctccg atgataagcc ccgatagatg
    31741 gctgagtttc ccggctcttt tatacgtcca catcatgcgg tcgaggcgat acaaatattc
    31801 tcccacatct tcgatgaaaa gaattttttc attggtatcc aaatcggagc aacttcccag
    31861 aatgctggtg agtatggaaa gatttccgcc tgtaagaata cccctcgcct tacctttacg
    31921 gttgagggca tgaggtgcta agctatattt cggaagattt ccctgtaaaa aactcaacac
    31981 ttttaaagcc tgacttggat tttctataga aaatgccgaa gccatgggac tgtgcagact
    32041 ttgccatttc agacaatgca aatgagcatg taagagggtg atatcgctaa atccaatgat
    32101 ccatttggga aacgtttcga aggaacgaaa atcgagcctg tcgatgatac gatgaagccc
    32161 atatccacct ctcgacatga gaatagcttt gcagtatgga tcgtccaagg cctgttgcaa
    32221 atcttcgagg cgttcgtcgt cggtaccgct aaattggtaa aatgcaccca aagcatggga
    32281 catgatttct acttcgaatc cataattctt gaaaacttta tcggccttca ataccttttc
    32341 gggcgcaaca cgtcccgctg gtgatgtgat gcaaatctta tcgcctgaat ttaaataggc
    32401 aggccttcta tataaatgat tcattcgaat gaatagaaat gctaaagtat aaatttttca
    32461 atcgaccata tatcgtcata aaagatttat tttcggtata gacataccgg aattaagggt
    32521 gaggaaaacg agttgcaata tattggcaag caagccgatt atttatcttt gcaactcaaa
    32581 ttttcgagat aatggcttca gaaaataata aatcctataa acgtactacc attacttcgg
    32641 cattgcctta tgccaatggt cctgtgcata ttgggcattt ggctggagtt tacattccag
    32701 cggatattta tgctcgctat ctgcgttcga aaggagaaga agttttattt ataggtggaa
    32761 gcgatgagca tggcgtgccc attaccataa aagccagaaa agaaggtatc agcccacaac
    32821 aggtagtaga taagtatcac gagattataa aaaaatcatt cgaagaattg ggtatatcct
    32881 tcgatatata cagccgcaca tctttaccta tacatcatga aacggcatca aattttttca
    32941 aaaagttata tgatgagggg aaattcatcg aaaaagttac cgaacagtat tacgatgcag
    33001 aggtagggca atttttagcc gatcgatata ttattggtac ctgtcccatt tgtggcaatc
    33061 agggggctta tggcgatcag tgtgaacagt gcggaagttc actcaatgct accgatctta
    33121 ttcaacctcg atcggcctta agcggaagca tcccggtttt gaaagcaacc cgccactggt
    33181 ttcttccgct caatgagtat gaagcatggc tgaaaaaatg gatactcgaa gaacataagg
    33241 atgactggaa aataaatgtt tatgggcaat gcaaatcatg gctcgatcat ggattgcaac
    33301 cgcgggccgt tacccgtgat ctcgactggg gagtaaaagt gcccatccaa ggtaccgaag
    33361 gcaaagtttt gtatgtatgg tttgatgctc ccatcggtta tatctcggct acaaaacact
    33421 gggctcagct aactggcgga gactgggaaa aatggtggaa atcgcctgac acacgcttga
    33481 tacattttat cggcaaggac aatatcgttt tccattgcat catctttcca gttatgctaa
    33541 aagccgaagg aagctatatc cttcccgata acgttcctgc caatgaattt ctgaatttag
    33601 aaggcgacaa gatttcaact tctcgcaact gggcggtctg gctcgacgaa tatcttaaag
    33661 attttcccgg caaacaggat gtgctgcgtt atgtactctg cgccaatgct cccgaaacca
    33721 aagacaatga ttttacatgg aaagactttc aaacacgtaa taatagtgag cttgtggcta
    33781 tattcggaaa tttcgttaac cgaacggctg tgctggcaac aaaatttttc gacggacggg
    33841 tccctgagct taccagtctt cttcccatcg atgaagaaac gatggcagag attgcagcta
    33901 ttcctgcacg tattgcctcg agcctcgagc attttcgttt tcgtgaagcc ttgaatgaaa
    33961 tgatgaactt ggcccgactc ggaaataaat acctcaccga aaacgaacca tggaaaatag
    34021 cctcttccaa catggaaaga gtaaaaacag ttttaaatat ttcgctgcag atttgcgcca
    34081 acctcgctat tttgtgcgaa cctttccttc cgtttacctc gacaaaactt cgtcgtattc
    34141 tcaactttaa tgatggcaag tggaacgacg ccggacgttg tgatcttttg ccctcctctc
    34201 atatcctgag cacccctgag ttgcttttcg aaaaaatcga ggacgaagct atcgaaaagc
    34261 aaattcaaaa actcgtaaaa ataaggaata cggcttctga aacacccaag ctggaaccag
    34321 ccaaagatga aatcgatttc gatacatttt cgaagatgga cattcgggtg gccaccatat
    34381 tggaagccga aaagattgca aagacgaaaa aattgctaaa actaaaggtt gatacaggct
    34441 tagataaaag aacgattgta tcgggtattg ccgaatatta ccaacccgag gaaatcattg
    34501 gcacacaggt ttgtatcctc atgaatcttg caccccgaat catcaaggga atagaaagcc
    34561 gcggaatgat cctcatggca caagaccctc agggaaaact ctatttcgtt tcacctgccg
    34621 aaatcattcc cgctggcagc cagatacaat aagatgtggt atggcctcat tttagaggtt
    34681 tattgattct tctaattgaa ccctttcatt tgtttataaa atgctgttcc taccatttgt
    34741 tctatgcggg atacatcttt tcttccaaaa cgatttttct taccatttta gtaatatcat
    34801 tttcaataat ctttaacaaa taaggtttta tttctaatat tgtatcaaca aaacacaatt
    34861 agggattata gcaccaatca ggttgttcga ataacgtttg gcaataagaa acgtagggca
    34921 tttcgaagct cctacctgtc aagaaaacca agccgtttat taaatgttat gtctttcaaa
    34981 tttagcactt tccgccctat gttttttatt gcgtgttggc agtatgtgct ttttaattcc
    35041 atttccaatt tttaggagga atcaattcac accaataagg atcaacatta attttctgtc
    35101 ctgcttcata attagttgat tgtttttttt taattggtat ttttaaaagt tcaacaatca
    35161 attcactatt ttcatgttta atcactttgg ctattgctct gacggattcc ttctctgttt
    35221 taagaaactt tttctttgtt acagtaacat atacgacaat tgtatcatca attggataat
    35281 attttaataa ctcatttgat tttgtcatat cagattccat tttctccacc aattctttat
    35341 ttgtcatctg aagtaaagag gagtcaactt ctctttttaa ttcatatggt atatgattta
    35401 agtaattgta atattctgaa agtataatac ctgaaattat gtctggatga ttaattccat
    35461 tttcaataaa atattttttc aaatcactgt ttccccataa cccccaatta tttctaatcc
    35521 acattccgag tccaaacgag tatagttttc caaatacaac atctgaagga agggtcatgt
    35581 atgtatattt tgttgtgtcg tcaaacattt tatctaaata ttcgaaactt tcttttaagt
    35641 cttttggttt cccttttaca tttttccact cctgaccaaa agagtttata gaaaaaaata
    35701 ccaatattat tataaaaatt gtcttcatgt tcctttctct ttgcaatact gccatacctt
    35761 tttgggcttt gctttcgggc gggtttggct tcgctgactt tcagtttgga gcacaaaact
    35821 gtcaaccaac atagaacttg aatagtagca caaaactcca agtttacacg tcgccccgcc
    35881 tgacgcaaaa cggcttttag gtgttgccat tcctttcatt cgtccgttgg ttttagtctg
    35941 tttagttgtc ttgccacaaa gtcttgctgc cacttgtttt taaaatcaaa ccattgctgt
    36001 ctgaagtcgc ctgagttgtc aattacgagc ttaaattctt taaatggttt attctttctc
    36061 aatgcgtcaa acagtcggct ttgcaaattt ttgtcggtaa gttggtcagc gaactcactc
    36121 attgtttcaa acgcttcgct tgaagtccat ttgtcaattt cgtagtagtc ggtgaagttg
    36181 ttttctaatt gttcaagttc ttcgtcccac gagtcaaggt caatgtcggg ataattgttg
    36241 ttatctggca cgaaaaggag ttgtcttgat gttttgtgta tgaacgcacg aaaaccgcag
    36301 tcaagttgct ctgcaatttc ttttactatg tctgttgtct tgttggtcat attttttgtt
    36361 caaattaaaa aaattttttc tgttgcaaag cggtagttgt tatcatagtg agttgtctgt
    36421 ccggccgttt atgaagtcct taaagacctg aattgctgac ccccgatact gaaagtaggg
    36481 aagaagcaca aatgttgaaa aatcgtcaaa gtcagccgaa aagagtctaa acggggggca
    36541 gtcataagat ggtctaatga gtctggacaa tatttttatc aaagttaatt cattatgttg
    36601 tgagatggtc taataagtct ggacaatatt tttatcaaag ttaattcatt atgttgaaaa
    36661 gagggggtgt aggggagact cataacttga ttttttgtta ataaatcatg cagcattctg
    36721 atatacaagc attggcgggt tgttctttag gaaagaagga tactacttta tattgtaatg
    36781 gtgattaatc tggcaatttc taatagttca cctgggaaac atatgctcat ttgcccttct
    36841 tcacaatcaa ccctctcttt gtgtatatcc agggaaatca aagaaccttt ttttaaccca
    36901 ttcatggcta ccgatgtatc attacatgcg tatgccttga taaaatctga gcaagctaag
    36961 atagggtctt cgcctctatg gcaatcttcg ctttcaattc agccgtaaat gttcttcttg
    37021 actttttcat ataacagaaa ttataaaatt actaaattcc tgtccagttt ttttagacca
    37081 tcacgccact aaccatataa aaaaaacttg ctgcaactat tagaaagttt tgcagtttca
    37141 agttgttgtt tgttacaaac caataaagta taaaaactat tggtaagaaa attgcaaagt
    37201 caattgagtt aaagagcata aattctgtct attatattat tgtttattat ttacattgtc
    37261 gttttcttgg catgacgcac aacgttttgc cgcttgccgc aggtggcgat ttagaagcac
    37321 taagctgtca acctgcactg aagctgaata ggagcataaa ccttcaatta accacgtcac
    37381 cgccacttgc ggcaagcacc tatgtaaaag cagtaaattg agtccatcat taattttaac
    37441 ctgtagt
//
LOCUS       DAOORU010000007        37233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_1286_length_37233_cov_5.605062, whole genome shotgun sequence.
ACCESSION   DAOORU010000007 DAOORU010000000
VERSION     DAOORU010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 37233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37233
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1286_length_37233_cov_5.605062"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..1100
                     /locus_tag="PLF20_01200"
     CDS             144..1100
                     /locus_tag="PLF20_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX79659.1"
                     /translation="MAPVGLGCAFHLDSFENPIIESISFLQTHYIYVPHNRSHFCVGF
                     MDMHTMKMIDSLGPLITPPPPRPQMINDTLWVRNIPLNLKKFQGYDSLLIMIIPLGYY
                     PTLTRDIGPALTCDNCPAPSGHKHSYRYFFELGYFESWFYGEFYIELTILDGVTGKRY
                     VLKPGEKPAYNIFRWQETLPLNYQLMTAEHLADTFWMDTEVSKGWYDYFIEATYSKAQ
                     TYYSDTVRVYFPTSEGVEESTSGRMRIWPNPVRDVLFIESKEPIIRIEVYNMQGIRVM
                     QEALSEVSSYRLSMQKLHSGMYHVCMFDKKGSRSVTNVVVRR"
     gene            1325..2719
                     /locus_tag="PLF20_01205"
     CDS             1325..2719
                     /locus_tag="PLF20_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOX79660.1"
                     /translation="MAEKFSRRDVLKVLGLTLGGTLLPKISWPRDEPVYPLILPEEPA
                     VPLSKPITAITLGAGSRGNVYGNYALYYPEQLKIIGVAEPIPIRNERYSKLHHITDEN
                     RFVTWEQVFERPKFADAVLITTPDRLHYGPAMAALKMGYDLLLEKPIAPSEKECLDIL
                     ELTEKTGQIVAVCHVLRYSPYFIKLKELVQKKAIGELISIQHMEPIEHIHMSHSYVRG
                     NWHNSKESTPIILAKSCHDLDILRWIVVKPSKRIQAFGDLSWFKKENAPRGATPRCMD
                     GCAIERNCPYSALKIYYRERGWLYVFDLPDEKEKEGDAILEYLRSTNYGRCVYDMDND
                     QCDHYITNIEFEGGITASFSMEALTPWGGRRTRIMGSMGYIEGDMDRLTLYDFRTDKS
                     ETLESKAIDVENYEHAGHGGGDWRLVSNWVQAVDQHNPNLLTSTISQSIESHIMGFRA
                     EKSRLNGKVEKIDM"
     gene            3078..3371
                     /locus_tag="PLF20_01210"
     CDS             3078..3371
                     /locus_tag="PLF20_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79661.1"
                     /translation="MDLIIFISVYLISCFVIAFMAYKKKINWWLALIISVIVTPVGGF
                     IYYQSNNPIKAYKEVRYKCQRCGFYFTEKATFCPFCEHDGHKIPLKKIYVDMT"
     gene            3383..4249
                     /locus_tag="PLF20_01215"
     CDS             3383..4249
                     /locus_tag="PLF20_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOX79662.1"
                     /translation="MHKFTRIFYLIFIYTVLSGIQSYSQNSLSENLSLGVMTFNIRLD
                     TREDGKNAWPYRKAAVIEMLQNTHPAIIGFQEVLHHQVEEIAKGLRGYVHFGVGRDDG
                     KQGGEYCPIFFDSKVFSLIDSKTLWLSETPDLPGSLGWDAGCKRLVSVVLLKEKSSGK
                     ELYVLNTHFDHQGEKARIESAKMISRLCQEMIQNAPVILLGDLNASSETEIYRILTEQ
                     SSLKDAQTIAERKDEVDNCTFTGFDGDSCEHIDFIWMPQNAKVNLYKIILNQTPQGWL
                     SDHRPIYCKIEL"
     gene            complement(4252..4644)
                     /locus_tag="PLF20_01220"
     CDS             complement(4252..4644)
                     /locus_tag="PLF20_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79663.1"
                     /translation="MSNTHQFIDKLFSSLMFVLLLAACSSTQQTATQRSQINYSPGVE
                     SSPKGVLSESEKQVLDDFIARKIELIRKQQALVPGADTGNEDDSKQLAELQRQMQQLD
                     NEINAYLIRGERRDYYYDALQKAMKDFR"
     gene            complement(4652..6820)
                     /locus_tag="PLF20_01225"
     CDS             complement(4652..6820)
                     /locus_tag="PLF20_01225"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOX79664.1"
                     /translation="MLKLIRFLMICARHRFILLGILLLSFGNSYADEGMWLPLLLEKL
                     NQRDMQMKGLKITARDIYDVNHSSLKDAIVQFGGGCTAEVVSDQGLLLTNHHCGYGYI
                     QRHSSVQQNYLEEGFWARTLEDELPCTGLKVSFLIEMRDVSQTILENLTPEMNEKTRA
                     KTISQISGQITKEAIQGTHYQAEIEPFYNGNQFYLFIYEIFRDVRLVGAPPSSIGKFG
                     GDTDNWMWPRHTGDFSVFRIYADANNLPADYSPNNRPYQPRRFLPISLKGIEKGDFTF
                     VYGYPGSTREYLPSFVIRSITEQQNPVRIHLRDIRLRIMDEYMQVNKTICIQYASKSA
                     GLANGWKKWTGENRGITRINAISRKQDYESNFSRWAQHGPYNELLPLYEKKYLEYAYY
                     RVSELWLSEAAFGIECLSIARSFQTLVDASVKPRPDTAFIRKTKKQLLASLPSFYKNY
                     YPPLDFEMMKTLLDEYQKNRQNILFPISLDNWNQKYGGRLSAIFDKSLFPHQERLLGL
                     LKNYKPSDARRILKDPIYELASSLSSFHKNELQARVKPLQQSLDSLQRTYMKAQMEME
                     PDRLFYPDANSTLRVAYGNILDYYPRDAVHYLHQTTLKGVIEKEDSVIADYQVPIRLK
                     QLYEQKNFGRYADKSGNMPVAFIANNHTSGGNSGSPLLNAEGQLIGLNFDRNWEGTMS
                     DLMYDPNLCRNITLDIRYCLFIIDKYAGASRLIDEMSIVQ"
     gene            complement(6850..7818)
                     /locus_tag="PLF20_01230"
     CDS             complement(6850..7818)
                     /locus_tag="PLF20_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOX79665.1"
                     /translation="MKTYEETESIQQLHDVYEKMKAEIGKMIIGQHETIEKVITCIFS
                     QGHCLLIGVPGLAKTLLVNTVAKVLGLSFNRIQFTPDLMPSDIIGTEILDENRRFRFM
                     KGPVFAHVILADEINRTPPKTQSALLEAMQEKSVTVAGTTYSLDNPFFVLATQNPIEQ
                     EGTYPLPEAQLDRFMFNIWLDYPSLKEEINIVEATTGAGLQQPSQLLDAQQILYYQDL
                     IRQLPVTPIIYEYAVKLARSTRPGPGASLFTSKYITWGAGPRASQYLILGAKCHAALD
                     GRYAPDVDDVRAVATSVLRHRIVRNYKAEAEGISVDDIIRHLMETR"
     gene            complement(7847..9193)
                     /locus_tag="PLF20_01235"
     CDS             complement(7847..9193)
                     /locus_tag="PLF20_01235"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX79666.1"
                     /translation="MDLFLFVTSVGLFAQESAVIDEIVAVVGNKVILLSDIENQYIQY
                     RAQGNIRGGAEQTKCAILESLLFNKLLLNQADIDSVEVTDKQVESELDRRIKYFIQQI
                     GSKEKLEEYFKKSLNEIKNEFREIIRDQMRMEMVQYNLTKNVKITPTEVRSYFKKMPS
                     DSIPMVNSEYVIGQIVKKPKTSLADEIEVKEKLRALRKRILEGENFATLAVLYSEDPA
                     TAARGGEVGLYGRGELYPEYEAVAFKLKEGEISDIVQTKAGYHIIQLIERRGDYVNTR
                     HILMTPKISPEELQKAATFLDSVAQLINNKKITFEEAVDKFSDDPNKMNGGLMINPVT
                     GTTRFEPDHLDPKVFYVIDKLEPGQVSGPVPFKTDEGKDAYRLLYLKEKSKPHRANLK
                     DDYDRIQNWALEYKKDQVLQKWVKERIRQTYIFINPAYANCQFQHQWTDRTGEDYK"
     gene            complement(9232..10092)
                     /locus_tag="PLF20_01240"
     CDS             complement(9232..10092)
                     /locus_tag="PLF20_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79667.1"
                     /translation="MKCKIFLFSCLMILCFAGCNYIKKKFRPNETILARVHDDYLYSS
                     DIKGIVPEGTPREDSILIVKNFIENWVRNRVELHHAEKNLSADEKNFKEQLDDYRNSL
                     IIYTYESHLIRQKLDTMVSIEEIEDYYRKNKQNFVLREPIVKMIYVKISKDSLRAAQL
                     ARKFLQPDTILDYDAFERFCLTRTSDYYISINNWFLFRDLQNMAPITTFNEELFLKNN
                     RLIDLTDDQWRYLVRIYAYVTKDGISPLSMEMENIKNIILNTRKQELIRNMRENLYEK
                     ALKSDDIEFY"
     gene            complement(10366..12402)
                     /locus_tag="PLF20_01245"
     CDS             complement(10366..12402)
                     /locus_tag="PLF20_01245"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX79668.1"
                     /translation="MRRKTFGFIAVFVGLSLIICKTHAQNSSDPVLMTIDGQPVRVSE
                     FLNIYSKNNVQSEVADKKSMEEYLELFINFKLKVRDAEVNGLDTVKVLKDELAGYRKQ
                     LSAPYFIDSVVFRKLVNEAYERMQWDLRASHILLRCDKDASPSDTLATWKRMDEILRK
                     ACSGEPFERLAIQYSEDPSARDREATSQHPFIKGNRGDLGFFTVFNMVYPFETGAYRT
                     QPGKISDIVRTDFGYHIINVTDKRSALGKAQVAHIFFQIPPKASHSDSLRIQARADSV
                     YQLLANGADFSKLAKTLSDDKGSAMHDGVLPWFTCNRLVPEFVEVVYPLKNGEIAKPV
                     LTSYGWHIIKMIDRKPVASFDEVYNDLIQRIEKDMRYNLCKQSLINKLKTEYQLSHYP
                     KALSDFYNLVDDSIFVGKWQMPPAKKLKKQLFRFDDKKYSQADFAQYLYKNQKKQPAK
                     DKITYVNNMYKAYVDQEILAYEDAILETKYPEFGLLMKEYHDGILLFEITQKKVWDKA
                     IRDTTGLQQYYEKIKQNYMWPQRKEATVVMIKNIADAQTAKQLAKQITEWINLADMTT
                     ETIKDKILQDSMLLVDINREKFLDGDNPMVDRLHKPGEMTQDVSPDESGKFNLNLVIL
                     HRILDPEPKDLADIRGHITAEYQNYLENEWLKELRSKYPVEVDHKIFETICARN"
     gene            complement(12441..13907)
                     /gene="guaB"
                     /locus_tag="PLF20_01250"
     CDS             complement(12441..13907)
                     /gene="guaB"
                     /locus_tag="PLF20_01250"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008449700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOX79669.1"
                     /translation="MKERTARVEREGLTYDDVLLVPSYSEVIPREVDISSYFSRNIAV
                     KIPIVTAAMDTVTESTMAIAIAREGGIGVIHKNMTIEQQALEVRKVKRAEAGMILQPI
                     TVNVNARVADAINIMREHKIGGIPVVNDDLILVGIITNRDLRFERNIQKSVSEVMTQK
                     VITTEEFTDFETAAEILNQYKIEKLPVVDKQGHLIGLITYKDIIKLKSMPNACKDDKG
                     RLRVAAAVGVARDTLERVNALVAEGVDAVVIDTAHGHSKAVLEMARQVKSAFPDIDIV
                     VGNIATRQAAEDLLKLDIDAIKVGIGPGSICTTRIIAGIGVPQLTAIMEVYDVIKDTP
                     VPLIADGGIRFTGDIVKALAAGASTIMAGSLFAGVDESPGEAVIYEGRKYKTYRGMGS
                     IEAMQKGSKDRYFQDMEDDIKKLVPEGIVGRVPYKGSLNEVVTQMVGGMRSGMGYTGC
                     KNISELRKATFIRITQAGIIESHPHDVTIISEAPNYSR"
     gene            complement(14021..15928)
                     /locus_tag="PLF20_01255"
     CDS             complement(14021..15928)
                     /locus_tag="PLF20_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOX79670.1"
                     /translation="MAANGDVTIYWQPGGSVIDFQRNEVYYSSDIFSSFDLIAQIPDL
                     EILHYRHIGANANAVHAYYFVRTITTTGLSFDSDTIAAILPVGSILDQTYIQLQWNAL
                     MDTLPSSCDGWYRIFCREEGGKWTKIDSTTDLSYIHEVRPCVDRYYKIEVDDSYCGSV
                     SAIAKVDHDIEQPPTPIFDSVSVVNGRIHLGWQEVNAPDAAGYIVYLQDQGIWDTLAL
                     IPDAHTTFFIDTLNDPSVKSWKYSVAAFDYCGNVSEDMGISQAQGSILLAEPIFEVCL
                     GRIRLHWSSYTNLPGGLGGYTVYLSENNNTFYPFVHLNASTLTYDFIQPQDKVKYSFF
                     IRANSINGQISASSNVRYLHVRIPPQPEYAYIRYGTVVNNQSIALGFHVDTAAPIQKF
                     FLQRMTNYSGNFAQVKEFTQKDAVIYFIDSAVNVSKNQYHYRLIVLDSCGEETLVSNL
                     ASNILLKNPHSSDLQWNAYRDWYNGLEGYEVYRIEFLDTVLVAKLNPDDTIWSDPNID
                     RETGAMYFIRAIEAHGNPFFFKEISQSNTVVIEPDYHFYIPTAFIPFRSTVGHIFKPF
                     IMAIDPTEYYFAVFSRIGQMIFETHSLDEGWDGTCKGKKVESGVYVYYIRFLTRKGKY
                     FDRSGTFVLLD"
     gene            16286..16939
                     /gene="rpe"
                     /locus_tag="PLF20_01260"
     CDS             16286..16939
                     /gene="rpe"
                     /locus_tag="PLF20_01260"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOX79671.1"
                     /translation="MTLIAPSILTTDFSKLSDTISMLQLSEADWIHLDIMDGVFVPNL
                     TFGFPVIRAIRKHTTKPLDVHLMMVEPEKYFKEFREAGADILSVHVEVSPHLHRHLTT
                     IKQLGMKAGAVLNPHTSLVNIENVLHQVDIILLMSVNPGFGGQVFIPQTIEKIRKLKK
                     MLLESGSKALIEVDGGVGLDNAQSLIDAGVDILVVGNSIFSQSDPQLAIHQLKHSKR"
     gene            16936..17955
                     /locus_tag="PLF20_01265"
     CDS             16936..17955
                     /locus_tag="PLF20_01265"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOX79672.1"
                     /translation="MKKLLILGLILICLLARAQENQLIVMFYNTENLFDTEDDPEKTD
                     EGFTPEGNYHWTKARLYKKLNNLARVILASGEWDNPSIIGLCEVENRNVLQMFTQYTA
                     LKSVGYRFIHYESPDPRGIDVALLYNPDDFMPLISRPIRVKNEKGELLQTRDILYVKG
                     LVRDIDTLHIFVNHWPSKYGGVAGSLPRRFAAALTLKQQVDSLLHQGEANILIMGDLN
                     DNPFDISVKDVLQACIDTANCESSLINLMAPLMKTQNAGSHKFQGQWDIIDNIIVSKT
                     LYEGKLHLRARKAKIFAPPFMLTKDKNYTGKKIYRTYNGMKYEGGYSDHLPVITFLAV
                     KRPEI"
     gene            complement(17997..18977)
                     /locus_tag="PLF20_01270"
     CDS             complement(17997..18977)
                     /locus_tag="PLF20_01270"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HOX79673.1"
                     /translation="MANLNTNYMGLNLRSPIIAGSSGLTSSLKNIVEIERQGAGAVVL
                     KSIFEEQIRFEAHQAHSFQQSPYPEAWDYIHQYTQSHELDKYLRLIFDAKENTSFPII
                     ASINCITAAQWTSFARQIEQAGADALELNIFILPSDPFRSSEENEKLYFDIAKAVLRE
                     LNIPVSIKLSWYFSSLTRTFLQLSWTGIKGMVLFNRFYSPEIDIDTLEVTSGHVFSQP
                     GELNLPLRWIAMLSDRMHCDLAGSTGVDSGEEALKLLLVGAKAVQMVSALYRYGFSRI
                     ADTNQQIGSWMDQRGYESLEQIIGKMSLKNIENPASYERVQFMRHFAGVE"
     gene            complement(19334..20035)
                     /gene="radC"
                     /locus_tag="PLF20_01275"
     CDS             complement(19334..20035)
                     /gene="radC"
                     /locus_tag="PLF20_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOX79674.1"
                     /translation="MKKKEESKSIHQWAEDDRPREKMLKKGPDSLSNAELLAILISSG
                     NIEESALDLAKRILQDHQNNLTALSRLELSDLMKYKGIGMAKAVTIKAALELGKRRLS
                     EEAFEQGKIQSSKDAYLIFRKYLEDNHYERFYALFLNKANSPLGEPILIGEGGTDGTV
                     VDPKRIMRLALEKFASSLIIAHNHPSGNLQPSAADINLTEKIAAAGKLLDIVLLDHLI
                     IGINNYYSFAEARKL"
     gene            complement(20302..22578)
                     /locus_tag="PLF20_01280"
     CDS             complement(20302..22578)
                     /locus_tag="PLF20_01280"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005802162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOX79675.1"
                     /translation="MKKFLLIINLLAFSWTFGQGNSKYVNPFIGTDKMGHTFPGATVP
                     FGFVQLSPDTDTIPYNDGNVYNPQVYQYCAGYQYNDPTIVGFSHTHLHGTGHSDLGDF
                     LLMPVSGKIKINPGIASKPGSGYRSRYDKSREKAEAGYYRVCLTDYDIDAELTATQRT
                     GVHRYTFHRADSCYLLLDMTHGIYNYDGKVVWSSLHVVNDTLVTGYRQTNGWARERFI
                     YFAMTFSKPVQEYNLLNLDKEIYTGFWRRWKMDHNFPERSGRKLKAIFKFDIRSGEQL
                     VVKMGISGVSEQGAMKNLLTEAAPFTFDEIRKQATQSWDAELNRVKIVATNEEKIVFY
                     TALYHSLLCPNIYQDVDARYRGLDHEIHTTQGFTNYTVFSLWDTFRALHPFLLLYHPS
                     QANDMIRSMLAHASQSVHGMLPVWSHHANENWCMTGYHALPVVADAWVKGIRDFPPDQ
                     ALKDMLQTATYKSYDGLGEYMQLGYVASETSPTSVSTTLEYAYDDWTLSQMALALGKD
                     SLAKIFKNRSLNYRNVFDYETGMMRPKKRNGHFVKPFDPLSTHNQGFIEGNAWTYSLF
                     VPHDIDGLIQLMGGANKLEAYLDTLFTMSLPEEYYENTEDITKDGVMGNYVHGNEPGH
                     HIPYLYNYTSHPWKTGLQVEKILKTFYRNSPSGLCGNDDCGQMSAWYIFSSLGFYPIC
                     PGSTEYTIGRPAFEEINFQLENGHNIHIVTKNFRSDIPYVQQVLLNGKPLSTPFIHHS
                     DLKDGAQIEFIMGKSPKI"
     gene            22735..24591
                     /locus_tag="PLF20_01285"
     CDS             22735..24591
                     /locus_tag="PLF20_01285"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOX79676.1"
                     /translation="MSSIIESASYNEESIRSLDWREHIRMRPGMYIGKLGDGASHDDG
                     IYVLLKEIMDNAIDEFVMGFGKTIEVNIQDRRVNVRDYGRGIPLGRLLECVSKINTGA
                     KYDSKVFKKSVGLNGVGSKAVNALSSWFQIQSIRDGKTKIVEFHKGLLIKEIEQSSDL
                     RSGTLVSFIPDEEIFGNYHFLNEHVERQLWNYAFLNNGLTILFNGQRLQAKNGLADLL
                     EVNMETEPLYPIIHLKDADFECAFTHDSSHYGEEYYSFVNGQHTIQGGTHQQAFREAY
                     VKTIRDFFKKDYDTSDIRTGLIAAISIKIQEPIFESQTKTRLGSTLMEPEGQSVSKYI
                     HDFLKKHLDDYLHINPATAEAIQRKILQSEKERKEMAGIKKLARESAKKVSLHNKKLR
                     DCRIHYNSNDPRKLESTLFITEGDSASGSITKSRDVNTQAVFSLKGKPLNSYGLSKKI
                     VYENEEFNLLQSALDIEDGLENLRYNQIVIATDADVDGMHIRLLLLTFFLQFFPDLVK
                     AGHLFILQTPLFRVRNKQHTFYCYNEEEKEEALAKTGKNPEITRFKGLGEISPEEFRH
                     FIGPQMRLDPVILRKDQAINEILAYYMGKNTPQRQAFIIENLRYEADAIFID"
     gene            24790..25074
                     /locus_tag="PLF20_01290"
     CDS             24790..25074
                     /locus_tag="PLF20_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79677.1"
                     /translation="MDREKFKENAKKNIDDLFAKIDEMEAKKDKVKAETRAEYEAKIN
                     ELKAKKDELQKKYEALSEATDEKWDEVKDTFSSAMDSFKEGFSKIASIFK"
     gene            complement(25187..26242)
                     /gene="rfbB"
                     /locus_tag="PLF20_01295"
     CDS             complement(25187..26242)
                     /gene="rfbB"
                     /locus_tag="PLF20_01295"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOX79678.1"
                     /translation="MEKKILITGGAGFIGSHVVRLFVNKYPQYHIFNLDKLTYAGNLE
                     NLRDIEDKPNYSFIRADIQDNDAIMKLFVLHRFDGVIHLAAESHVDRSISNPLEFVMT
                     NVVGTVNLLNAARFIWKGNFEDKRFYHISTDEVYGSLGSIGLFTEETPYEPHSPYSAS
                     KASSDHFVRAYHDTYGLPTVISNCSNNYGAYQFPEKLIPLCINNIRFNLTLPVYGKGE
                     NIRDWLWVEDHAAAIDLIFHRAPNGETYNIGGNNEWKNIDLIRLLCRIMDRKLERPSG
                     TSEKLIAFVKDRAGHDLRYAIDSSKLQEQLGWKPSVTFEEGLEKTIDWYLSNPEWLAH
                     VTSGEYQKYYEEQYIKR"
     gene            complement(26266..27294)
                     /gene="galE"
                     /locus_tag="PLF20_01300"
     CDS             complement(26266..27294)
                     /gene="galE"
                     /locus_tag="PLF20_01300"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOX79679.1"
                     /translation="MKILVTGGTGYIGSHTVVELQQKGYDVVVVDNLSNSRIEVLDNI
                     EEITGIRPEFYQLDLSEQEPTFQLFKEHSDIAGIIHFAAYKAVGESVEKPLLYYRNNL
                     QSLINILEGMLDNRIANLVFSSSCTVYGQPDELPVRETSPIKEAISPYGNTKQISEEI
                     IRDTIHAFPQLKAISLRYFNPIGAHPSALIGELPLGIPSNLVPFITQTAIGKREVLRV
                     YGKDYNTPDGTAIRDYIHVVDLSEAHVVALDRMLQNKMKNNYEIFNLGTGQGHTVLEV
                     IRSFEKVSGVRLNYVFTDRRPGDVEKVWADTSYANQELGWKAKRSLDEMTLSAWRWEL
                     RLAGKLNE"
     gene            complement(27854..28801)
                     /locus_tag="PLF20_01305"
     CDS             complement(27854..28801)
                     /locus_tag="PLF20_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOX79680.1"
                     /translation="MYNFALIGAGGYIAPRHMKAIKDTGNRLVAALDPNDSVGIIDSF
                     FPEADFFLQPERFDRHLDKLRRQGEKKIDFVSICSPNFLHDSHIRLALRNQANAICEK
                     PIVLNPWNIDALEDIENETGFRIYNILQLRLHPQIIALKEKIKNGPTDKIYDLDLTYI
                     TSRGKWYHYSWKGDVEKSGGLATNIGVHFFDMLGFIFGKIRLVAVHHSSPTVVAGFLQ
                     YDKARVRWFLSIDSKYLPKEIAAKGQRTYRSILIDGEEFEFSEGFADLHTRVYEDILS
                     GGGYGLEDARQSVEIVYQIRNAKPFGLTGDFHPYLLKVD"
     gene            complement(28826..29404)
                     /locus_tag="PLF20_01310"
     CDS             complement(28826..29404)
                     /locus_tag="PLF20_01310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOX79681.1"
                     /translation="MAKDYFAHETAVIDKGCVIGQGTRIWHFSHIMSGCTIGENCNLG
                     QNVVVSPKVVLGNNVKVQNNVSIYTGVICEDDVFLGPSMVFTNVINPRSAVNRRGHYA
                     QTIVKRGATIGANATIVCGHNIGEYAFVGAGAVVTREVLPYALVIGNPARQIGWMSEY
                     GHRLHFDEDNIAICPESHEKYKLENNQVFKIS"
     gene            complement(29425..30561)
                     /locus_tag="PLF20_01315"
     CDS             complement(29425..30561)
                     /locus_tag="PLF20_01315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOX79682.1"
                     /translation="MRKIQMVDLYSQYVKIKPEIDQAIQEVIDSTAFIKGPVVKFFQK
                     DLEEYLGVKHVIPCGNGTDALQISMMALGLKPDDEVITADFTFVATAEVIALLGLTPV
                     LVDVDPDYFNIIPEAIEKALTPHTKAIVPVHLFGQCADMDAIMEIARRHHLYVIEDAC
                     QAIGSEYHSTDGSIKKAGTIGNIGCTSFFPSKNLGCYGDGGAIFTNDDELANMLRGIA
                     NHGMFARYYHDFVGVNSRLDSIQAAILKVKLPHLDEYISARQKAAQYYERAFADIPYL
                     KLPVRHPKSSHVFHQYTLICNGISRDDLVNYLAKKDIPAMVYYPVPLHLQKAYKSERY
                     GKGDFPVTEMLSANVFSLPMHTELDEEQLQYICDQVLEFISRVK"
     gene            30860..31630
                     /locus_tag="PLF20_01320"
     CDS             30860..31630
                     /locus_tag="PLF20_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79683.1"
                     /translation="MQKRKTIIFLILISSIFTFQKAEGQVNIADSMIRIFGVSARLGS
                     HLPAADMAERFGISYTVGPAFTYKTNKNWIWDAGFDYIFGNDVKIKDSLFRLISTSHQ
                     FIIDQEGVPADVAVQERGYHFYVSSGKLFPIWGPNPNSGLIVEFGMGYLLHKVNISVL
                     NNSAPQLRGDYKKGYDRLTGGFSLNQFIGYQHLSSKRIYNFFVGLELIEGFTSSLREY
                     DFDRMAPAEGNRFDMMIGLKAGWILPIYSRAPKDYYYY"
     gene            31676..32878
                     /locus_tag="PLF20_01325"
     CDS             31676..32878
                     /locus_tag="PLF20_01325"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOX79684.1"
                     /translation="MIKVSSIFNPKAKEWIKGRKNLFQRLQDKIDRDKPLVWFHCASL
                     GEFEQGRPLIEAFHQSYPSFKILITFFSPSGYNVRKNYIGADYIFYLPADTPVNAKKF
                     IEIIKPKLVIFIKYEFWLNYLKEIHARDIALLLVSGIFRPQQVFFKWYGKIFRQQLKA
                     FSHFYVQNETSAQLLKNIGFDNVSASGDTRFDRVETIAANSQPLHEIKTFCADKKVII
                     GGSVWPADLKILEPFIQQASENFKFILAPHEVNRDIISGLLEHYGDKAITYTALKNRP
                     QNDEKILIIDIVGILSSVYQFGWVAYIGGGFGVGIHNILEAAVYGIPVFFGPHYRKFQ
                     EACDLIERGGAFPINSSDDLLRIILNADFEKNRNKVAKICKEYVLQNTGATKIVMQGI
                     NKIIKNKG"
     gene            33328..33795
                     /gene="moaC"
                     /locus_tag="PLF20_01330"
     CDS             33328..33795
                     /gene="moaC"
                     /locus_tag="PLF20_01330"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF006870.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOX79685.1"
                     /translation="MGKLSHTDDKGKALMVDISHKPLEKRIAIATGQIFLQNSTIQLI
                     KENLIKKGDVLTTAEIAGIQAAKQTGMLIPLCHPLPLSDVEVATRIHEQSVEVTSKVI
                     TIWQTGVEMEALTAVSIALLTIYDMCKAVDKTMCMGNIHLVEKTKSSPDEKEA"
     gene            33779..35788
                     /locus_tag="PLF20_01335"
     CDS             33779..35788
                     /locus_tag="PLF20_01335"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX79686.1"
                     /translation="MKKRPKLKKSKFYFILFFSWGFMISSQAQINIPHFIFSGMHHLS
                     EGRFVDAIKSLNVVINAAPDMFEPYFYRGIAKYNLGDYQGAEQDFTRSIEINPMFSEA
                     YHYRAITRNRLRNYQAAMDDFKEALRIAPLSAEIYVNRSITRMGLKDYKNALIDVDSA
                     IKIKNDIPLAYLARGLIKHQLNDTLGAIADINKAIQLNYFFTEAYIRRGSIKYDIGDY
                     KGAIDDFTQAIKMEPDNPAVYFQRAIAWIKLENKNAAFDDLNKVLELDPYNALTYYNR
                     AIIKGELGDLKGALEDYNHVLALNPDNILAAYNRAITRHQLEDYHGAIEDYTKAINLF
                     PDFSKAYFMRAEAKRMLKDEKGAFEDRNKAIQIEETIAKTNDSILRLYTDSTYLASII
                     EFEADFRKTSAVDGRIQYQPVYVDLEQNFIVTYLYNDSLYVEQKRGEYWLKKIDDIGK
                     KVDYKLSFGLTNKETKLTPEQIALYLKNIDLSTKLNPSDPLAYFFMGILNGMIKNYQS
                     ALDAYNKALELDPGFSLAYFNRANTRLEMLEELKEQEPLPVAISFGNNMQQPRITEKT
                     PLPEYQEVINDYNKVIELNPDFSFAWFNRANIKARIADYESALQDYSIAIQLEPNLAQ
                     AYYNRALILIYQHNNQPACQDLSRAGELGIANAYNLIKRYCSLKN"
     gene            36083..>37233
                     /locus_tag="PLF20_01340"
     CDS             36083..>37233
                     /locus_tag="PLF20_01340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX79687.1"
                     /translation="MTDQPANNIIVIIGGGAAGMMAAIIAARKGAQVILLEKMNKPGR
                     KLSITGKGRCNITNTDSLREFLTHIDPDPNFLFNIFSRFFSKDIIDFFQQLGINTIEE
                     RGGRIFPENVSAMEIVNTLVKEMQRLGVKIILGAPVKQIICTNRNIKGVRLENGTFIQ
                     SHKIILATGGSSYPATGSTGDGYKLAAELGHHIRTPVPALVPIDVAGRVAPTLRGLTL
                     KNIKATVYNNGKIIADEFGEMLFTHFGLSGPIILSLSRKLNRIDFKNNKVDISLDLKP
                     ALDEKTLDHRLLRDFTQHGKMQAKTILKGLLPSSLIPVCLDLLQIEAKKPAHQITAEE
                     RKKLRLWLKDFRFQITGIRGFKEAIITSGGVSTLEINPKTLESKLVSGL"
BASE COUNT        11316 a         7765 c         7141 g        11011 t
ORIGIN      
        1 accattttac aggaaaaaat ttgtgtttcc cgaaagttga aagttaaaga ttcaacttgc
       61 tgtatacaag gaaattgcat ccctaaggct ctcaagttgg gttacgttat tacaattatt
      121 gtagcttaag acccgttccc tatttggctc cagttggctt aggctgtgct tttcatctcg
      181 attctttcga aaacccgatt atcgaatcta tttctttcct tcaaactcat tatatctatg
      241 taccccataa caggtctcat ttttgcgtag gttttatgga catgcataca atgaaaatga
      301 ttgatagttt aggtccgctt atcactccac cccctcctcg tcctcaaatg attaacgata
      361 ccctctgggt tagaaatatc cccttgaatt tgaaaaaatt tcaaggatat gattcacttc
      421 ttatcatgat aataccactg ggttattatc caactcttac tcgcgatatt ggtccggctt
      481 taacatgtga taattgtccg gcaccttcgg gacataaaca ttcttacaga tatttttttg
      541 agcttggtta tttcgaatct tggttttatg gagaatttta catagagctt actatactgg
      601 atggtgtaac aggcaagcgt tatgttctga aacccggaga aaaaccagct tacaatattt
      661 ttcgctggca ggaaacactt ccattgaatt atcagctaat gacggccgaa caccttgccg
      721 atactttctg gatggatact gaagtatcga agggatggta tgattacttc atcgaggcaa
      781 cttattccaa agctcagact tattattccg atacggtaag agtgtatttc cccacttccg
      841 aaggagtaga agaatcaact tcgggaagaa tgagaatttg gcccaacccc gttcgtgatg
      901 ttttgttcat tgaaagtaaa gaacccatta tcaggataga ggtttacaat atgcagggca
      961 tcagggtaat gcaagaagca ttgtcggagg tttcgagtta tcgactttcg atgcaaaagt
     1021 tacattcggg gatgtatcat gtttgtatgt tcgataaaaa aggaagcagg tcggtaacca
     1081 atgttgtggt aaggcgataa gtattgggat atttgatgaa agtcgaattc cagatttcag
     1141 gatggcggga atcgatagat tgaggcgtta ggctaaaagg ttgcctcggt acactgctcg
     1201 cttcgcttgc ggcgctcgat gaccgatatc tcgatatact attataaacc actcaacatc
     1261 tgacagagtt gataatagaa tgggattcca aaatttttat ttttgtcaga aaaacaaaaa
     1321 taacatggca gaaaaatttt cgagaagaga tgttttgaaa gtattgggtc tcaccttagg
     1381 cgggacctta ctacctaaaa tttcatggcc aagggacgag ccggtttatc cgcttatcct
     1441 gcctgaagaa cccgcagtgc ctctttccaa acccattacg gctataaccc ttggagcagg
     1501 ttcacggggg aatgtgtatg gcaattatgc cctttattat cctgaacagt tgaagattat
     1561 tggcgtggcc gaacccattc ccattcgtaa cgaaagatac agcaaactgc atcatattac
     1621 cgacgaaaac cggtttgtaa cctgggagca ggtattcgag cgtccaaaat ttgccgatgc
     1681 cgtcctcatt acaacccccg atcgattgca ttatggtccg gccatggccg ccctgaaaat
     1741 gggctatgat ttgctcttgg agaaacccat agctccttcg gaaaaagaat gtttggacat
     1801 tttggaactc accgaaaaaa cgggtcaaat tgttgctgtt tgtcatgttt tgagatattc
     1861 tccctatttc atcaaattaa aagagcttgt gcagaagaag gccattgggg aattgatcag
     1921 cattcagcac atggaaccca tcgaacatat tcacatgagt cattcgtatg taagaggtaa
     1981 ctggcataat tcgaaagaat ctacgcctat tattcttgcc aaatcatgtc acgatctcga
     2041 tattttacga tggatcgtgg ttaagccttc taaacgtatt caagcatttg gcgatcttag
     2101 ttggtttaaa aaagaaaatg ctccccgggg tgcaactccg cgctgtatgg atggatgcgc
     2161 catcgaacgc aattgtcctt attctgccct gaagatttac taccgtgaac gaggctggct
     2221 ttatgtattc gatttacccg acgaaaagga aaaagaagga gatgccatac tggaatatct
     2281 gagaagcaca aattatggcc gctgtgtgta tgatatggac aatgaccaat gcgatcatta
     2341 tatcaccaac atcgaatttg aaggaggtat aacggccagc ttcagtatgg aagcccttac
     2401 accctggggt ggacgtcgca cacgtatcat ggggagtatg ggctacatag aaggagatat
     2461 ggaccggctt acactatacg atttccgtac cgataaatcg gaaactttag aatctaaggc
     2521 catagatgtt gaaaattacg aacatgcagg ccatggcgga ggtgattggc gactggtatc
     2581 caattgggtt caagctgtag accagcataa cccaaatttg ctcacttcta ccataagtca
     2641 atccatcgaa agccatatca tgggtttcag agccgaaaaa agccggttga atggtaaagt
     2701 ggaaaagatc gatatgtaaa gaatttgctt cccgatcgat atatttaaac catgatcgac
     2761 catgtccatc acccgaacat catattgtgg aaaagatcgt ttcccgaaaa taattcaaca
     2821 ttttcatcct ctcgccataa ggatcgttta catctgtcag ttttatgaag aatctatatt
     2881 tttgactcat tctaaacaac gatattttca aatttattca ttgttaatta tgaataaaat
     2941 tgacggtcag cgtatagatg agctaataac acaagtcttt tggtcgaagg aaccaaccat
     3001 taattatggg gtattgatga attaaaaaca ttcatagctt tgcattcgga tttctaatct
     3061 atcgaatttc attaccaatg gatttaatca tatttataag tgtttatttg atttcatgct
     3121 ttgtcattgc cttcatggct tataagaaaa agatcaactg gtggcttgct ttaattatca
     3181 gtgtgatagt aacgccagta ggaggtttta tttattatca aagcaacaat ccaatcaagg
     3241 catataaaga agttcgttac aagtgccagc gatgtggttt ttactttacc gaaaaagcca
     3301 ccttttgccc tttttgtgaa catgatgggc acaaaatacc cttgaaaaag atatatgttg
     3361 atatgaccta aaactttttt tcatgcataa gttcacaaga attttctatc taatttttat
     3421 ttatacagtt ttaagtggaa ttcagtcata ttcgcaaaat tccctatccg aaaacctttc
     3481 attgggagtg atgacattca acataaggct ggatactcga gaagatggta agaatgcatg
     3541 gccatatcgc aaggctgctg ttatcgagat gttgcaaaac acacatccag ctattatagg
     3601 atttcaggag gtcttacatc atcaggttga agaaattgca aagggtttac gtggttatgt
     3661 ccattttggc gttggacgcg acgatggaaa gcaaggaggc gagtattgcc ctattttttt
     3721 cgattctaaa gttttttcat taatagatag taagacctta tggttgagtg aaacacctga
     3781 tttacccggt tcattaggat gggatgcagg ctgtaaacga ttagtcagcg tcgttttatt
     3841 gaaggaaaaa tcaagtggaa aagaattgta tgtgctcaat actcatttcg atcatcaggg
     3901 agaaaaagct cgaatagaaa gcgccaaaat gataagcagg ctctgccagg agatgattca
     3961 aaatgcaccg gttattcttc tgggagatct caatgcctcg tcagaaacag aaatctatag
     4021 aattttaaca gagcaaagtt cactgaaaga tgcccaaacc atagcagaac ggaaagatga
     4081 agtcgacaat tgcacattta cgggtttcga tggagattca tgcgagcata tcgactttat
     4141 atggatgccc cagaatgcca aagtaaacct ctacaaaatc attctcaatc aaacaccaca
     4201 gggctggctt tccgatcacc gacccattta ttgcaaaata gagctttaga tttatcgaaa
     4261 atctttcatg gctttttgca aagcatcata ataataatct cttctttcgc ccctaatgag
     4321 ataggcattg atttcattat cgagctgttg catttgcctt tggagttcgg ccaattgttt
     4381 cgaatcatct tcatttcctg tatcggcacc tggcaccaat gcttgttgct tccgtattaa
     4441 ctcgattttt cgggcaataa aatcgtcgag tacctgtttt tcgctctcac tcaaaactcc
     4501 tttaggagaa ctttctaccc cagggctgta atttatctgg cttctttgag ttgcagtttg
     4561 ctgagtggat gaacatgcag ccaataaaag cacaaacatt agagacgaaa atagtttatc
     4621 tataaattgg tgggtgttac tcatttttat attattgaac gattgacatt tcatcgatca
     4681 ggcgggaggc tccggcatat ttatcgataa tgaacaggca atagcgaata tcgagagtta
     4741 tattacgaca taaattagga tcatacatca aatcactcat cgttccttcc caattgcggt
     4801 cgaaattgag accaatgagt tggccctcag catttagtaa tgggcttccc gagtttccac
     4861 ccgaggtgtg attattggca atgaaagcca ccggcatgtt acccgattta tcggcatatc
     4921 gcccaaaatt cttctgttcg taaagctgtt tcagtctgat aggcacctga tagtcggcta
     4981 ttactgagtc ttctttttcg attacacctt tcagagtagt ttggtgaaga taatgcacgg
     5041 catcgcgagg gtaataatcg agaatgtttc cgtaagcaac cctaagcgtg gaattggcat
     5101 cgggatagaa caatcgatcg ggctccattt ccatctgtgc tttcatgtaa gtccgctgga
     5161 ggctgtcgag cgactgctga agaggtttta ctcttgcctg cagttcattt ttatgaaacg
     5221 agctcaatga agaagcaagt tcataaattg ggtcttttag aattctcctt gcatcggagg
     5281 gtttgtaatt tttcagtaag cccagtaaac gttcctgatg ggggaacaaa gatttatcga
     5341 aaatcgccga aaggcggccg ccatatttct ggttccaatt gtccagcgag attgggaaga
     5401 ggatattttg tctattcttc tggtattcat ccagtaaggt tttcatcatt tcaaaatcaa
     5461 ggggtgggta ataatttttg taaaatgatg ggagactggc aagcagttgt tttttggttt
     5521 ttcgaatgaa ggccgtatcg ggtcgaggtt tcaccgaagc atccaccagt gtctgaaatg
     5581 aacgggctat cgaaagacat tctattccaa aggcagcctc cgaaagccat agttcagaga
     5641 cgcgataata ggcatactcc aggtattttt tttcgtaaag cggaagcaat tcgttgtaag
     5701 gtccatgttg ggcccatcga gaaaaattac tttcataatc ctgttttcgt gaaatagcat
     5761 ttattcgggt tatgccgcga ttttcgcccg tccatttttt ccagccattg gctaaacctg
     5821 cacttttgga ggcatattga atgcagatgg ttttatttac ctgcatgtat tcatccataa
     5881 ttcgaagacg aatatcgcgc aggtgaatcc tgacaggatt ttgttgttcg gtaatagacc
     5941 gaatgacgaa agatgggaga tattcccgag tagaaccggg atatccataa acaaaagtaa
     6001 aatcaccttt ctctattcct ttcaaagaga tgggaagaaa acggcgaggt tggtaaggtc
     6061 ggttattggg gctataatcg gctgggagat tatttgcatc ggcatagata cggaaaaccg
     6121 aaaaatcgcc cgtatgcctg ggccacatcc aattgtcagt atcgccgcca aattttccta
     6181 tactcgatgg aggtgctcct acaaggcgaa catccctaaa aatttcataa ataaagagat
     6241 aaaactgatt tccattgtaa aatggttcta tctccgcctg ataatgagtt ccctgaatag
     6301 cttccttggt aatttgcccg ctgatttgcg aaatggtctt ggcacgagtt ttctcattca
     6361 tttcgggcgt taggttctcg agaatggttt gactcacatc acgcatttcg ataaggaacg
     6421 atacctttaa tccagtgcat ggtaactcat cctcaagggt tcgggcccag aatccttctt
     6481 cgagataatt ctgctgtacc gagctatgtc gttgaatgta gccataacca caatgatgat
     6541 tcgttaagag caagccttgg tcggaaacta cttcggcagt acaacctcct ccaaattgta
     6601 cgatagcatc tttcaaactg gaatgattta catcataaat atcgcgggca gttatcttca
     6661 ggcctttcat ctgcatatcc ctctggttga gtttttctaa cagaagagga agccacatac
     6721 cttcatcggc gtatgaatta ccgaatgata acaataaaat ccccaataag ataaaacgat
     6781 ggcgggcaca gatcatcaag aatcgaataa gtttcagcat aagtaatgat ttcttaaaac
     6841 attttttctc tatcgagttt ccatcagatg acggataata tcatccacag aaattccttc
     6901 ggcttcagcc ttataattac gaacgatgcg atggcgcaga acagaagtag caacggcacg
     6961 cacatcgtcc acatcgggtg catatcttcc atcgagggct gcatggcatt ttgcgccaag
     7021 gatgagatat tgcgaagcac gcggaccggc tccccatgta atatatttgg aggtaaacaa
     7081 agatgctccc ggcccaggac gtgtactacg agccagcttc acggcatatt cataaataat
     7141 cggagtaacg ggtaattgcc tgattaaatc ttgataatat aaaatctgtt gggcatcgag
     7201 caattgggaa ggttgttgaa gcccagcacc tgtggttgct tccactatat taatctcttc
     7261 cttcaaggaa gggtaatcca accagatatt gaacatgaaa cggtcaagct gggcttcggg
     7321 tagaggatag gttccctcct gttctatggg attttgcgta gccagcacga aaaagggatt
     7381 atccaggcta taagtggtgc ccgccactgt aacggacttc tcctgcatgg cttccaacaa
     7441 agccgattgg gtttttggag gggtacgatt gatttcatca gcaagtatga catgtgcaaa
     7501 aacagggcct ttcataaaac gaaatcttcg gttttcatcc aaaatttcag tacctatgat
     7561 atcggatggc attaaatcgg gtgtaaattg aatacgattg aacgaaagcc cgagaacttt
     7621 agccacggta ttaaccaaca gcgtcttggc tagcccaggt acaccaatca gtaaacaatg
     7681 cccttggctg aagatgcaag taattacctt ttcgatggtt tcgtgctggc caatgatcat
     7741 tttgccaatc tcggctttca ttttttcata aacatcgtga agctgctgta tggattccgt
     7801 ttcttcgtaa gttttcatga aaaagaatta actttattag atgttgctat ttgtagtctt
     7861 cgccagttct gtcggtccat tgatgttgga actggcaatt ggcataggca ggattaataa
     7921 agatataggt ttgcctgatg cgttctttca cccatttttg cagaacctgg tcctttttat
     7981 actctaaagc ccaattttgg atgcgatcgt aatcgtcttt tagattagcg cggtggggtt
     8041 tgcttttttc cttaagatag agcaagcgat aagcgtcttt cccctcgtcg gttttaaatg
     8101 ggacagggcc tgaaacctgg ccgggttcca gtttgtcgat tacgtaaaat actttgggat
     8161 cgagatgatc gggttcgaaa cgtgtagttc ccgtaacagg attgatcatc aaaccaccat
     8221 tcattttatt gggatcatcc gagaatttat ccacagcttc ttcgaaagtg attttcttat
     8281 tattgataag ctgagccaca ctgtcgagaa atgtggctgc tttttgaagt tcttcgggtg
     8341 agattttcgg ggtcatgagg atgtgtcgtg tattcacata atcgccacgg cgttcaatga
     8401 gttgaataat atgataacct gctttggttt gaacaatatc ggaaatctca ccttctttca
     8461 acttaaaggc aacggcttcg tattcgggat ataattctcc tctgccataa agtcctactt
     8521 cgccacccct ggctgccgta gccgggtctt cactgtaaag aaccgccagg gtagcaaaat
     8581 tttcaccttc gagaattcgc ttgcgcaaag cgcgcagttt ttcctttacc tcgatttcgt
     8641 cggctaaact cgttttgggc ttcttcacaa tttgtccaat aacatactcg gaattcacca
     8701 tgggaatact gtcggagggc atcttcttga aatatgaacg aacttcggta ggtgtaatct
     8761 tcacattttt cgtcaaatta tactgaacca tttccatgcg catctgatca cggatgattt
     8821 ccctgaattc attctttatt tcattcaacg acttcttgaa atattcttct aacttttctt
     8881 tcgaaccaat ttgctgaata aaatatttta ttcgtctgtc gagttccgat tcgacttgtt
     8941 tatcggtaac ctccacgctg tcgatatcag cctgattgag cagcagttta ttaaacagaa
     9001 ggctttcgag aatggcgcat ttggtttgtt cggctccccc tctgatattt ccctgcgctc
     9061 tgtattgtat gtattgattc tcgatgtcgg aaagtaaaat cactttgttt cctactaccg
     9121 ctactatttc atctatcaca gcactttcct gggcaaaaag cccaacgctt gttacgaaca
     9181 aaaacaaatc caaaaaaaat attctaaatt ttttcattat ttatcaaaca gttaataaaa
     9241 ttcgatatca tcgctcttca aagctttttc gtaaaggttt tcgcgcatat tcctgattaa
     9301 ttcctgttta cgagtattga gtatgatatt tttgatgttt tccatctcca tgctcagagg
     9361 tgaaatacca tctttggtta cataggcata tatgcgtacc aggtatctcc attggtcatc
     9421 ggtcaggtcg atcaatcgat tgttcttcaa gaaaagttct tcattgaaag tggtaatggg
     9481 ggccatattc tgcaaatcac gaaacaaaaa ccagttattg atactgatat aataatcact
     9541 ggtacgggtg aggcaaaacc tttcgaatgc gtcgtaatcg agtattgtat ctggctgcag
     9601 aaattttctg gccaattgcg cagctctcaa cgaatctttc gaaatcttca cataaatcat
     9661 tttaacaatg ggctctcgta aaacgaaatt ttgtttgttc tttcgataat aatcttcgat
     9721 ttcttcgata gaaaccattg tatcgagttt ttgccgaatt agatggcttt cgtaagtata
     9781 tataatcaaa gaattacgat aatcgtccaa ctgctctttg aaatttttct cgtcagcgga
     9841 aaggttcttt tcggcatgat gtagctcgac acgattacgt acccagtttt cgataaaatt
     9901 tttcacaata agtatactgt cttcgcgagg ggttccttcg ggcacaattc cttttatgtc
     9961 agaactatac agataatcat catgaactct ggccagaatg gtttcattgg gccggaattt
    10021 ctttttgatg taattgcatc ccgcaaaaca caatatcatt aaacacgaaa acaaaaaaat
    10081 cttacatttc attcgaatca atctatcact tttaaacatc atccacattg tatcataaaa
    10141 aatcaggtct tttattcgac ataacatctc aagcatataa cctcgccatg ctctatatat
    10201 tgcgaatccc ttcgggaatg gtttcgagtc atttgcctta tcttctcatg catgatataa
    10261 ttataagaat cgggttaatt cggcatcgtg atcatcgtga gaaaatgcca aggggttgcc
    10321 ctgatgaatg aagagaaaaa tcctgttcat caaaagcttt caatatcagt ttcgagcaca
    10381 gatcgtctcg aatatcttgt ggtcaacttc cacaggatat tttgagcgca attccttcag
    10441 ccattcattt tccagataat tctggtattc ggcggtaata tgtccacgaa tatcggccag
    10501 gtctttaggt tcagggtcaa gaattcggtg aagtatcacc agattcagat tgaactttcc
    10561 actttcgtcg ggactgacat cctgagtcat ttcgccaggt ttatgaagtc tatcaaccat
    10621 tgggttatcg ccatcgagaa acttttcgcg gttaatatct acaagtagca tagaatcttg
    10681 tagaatttta tcctttatgg tttcggtagt catgtcagcc aaattaatcc attcggtaat
    10741 ttgtttggca agttgtttgg ccgtttgagc atcggcaata ttttttatca ttaccactgt
    10801 agcttcctta cgctgaggcc acatataatt ctgcttaatt ttttcgtagt attgctgaag
    10861 tccggtagta tctcttatgg ctttatccca gacttttttc tgagtgatct cgaataaaag
    10921 gataccatcg tgatattctt tcatcagcag gccaaattca ggatatttgg tttcgagaat
    10981 agcgtcttcg tatgccaaga tttcttgatc cacataagcc ttatacatgt tgttcacata
    11041 agtgatcttg tctttcgcag gttgtttttt ctgattttta tataaatact gtgcaaaatc
    11101 ggcttgtgaa tactttttat cgtcgaaacg gaagagttgt ttttttaatt tctttgctgg
    11161 aggcatttgc catttaccga caaatatact atcgtcaacc aaattataaa aatcggataa
    11221 ggctttagga taatgagaaa gttgatattc tgtttttaat ttattgatca agctttgttt
    11281 acaaaggttg tatctcatgt ctttttcgat tctttggatg agatcgttat agacttcatc
    11341 gaatgatgca acaggtttac ggtcgatcat cttgatgata tgccagccat aagaagtgag
    11401 gacgggtttg gcaatttcgc catttttgag gggatagacc acttcaacga attcaggaac
    11461 cagtcgattg catgtaaacc atggcaaaac accatcatgc atggctgagc ccttgtcatc
    11521 ggaaagggtt ttggccaact tggaaaagtc cgctccatta gcgagaagtt gataaactga
    11581 gtcagcacgt gcttgaatgc gaaggctatc agaatgcgag gctttgggtg gtatttgaaa
    11641 gaagatatga gccacctgag ctttccccaa agccgagcgt ttatcggtta cattgatgat
    11701 gtgataacca aaatccgttc gcacgatatc ggagattttg cctggttgag tgcggtatgc
    11761 acctgtttcg aaaggataaa ccatattgaa aacagtgaag aaacccaaat cgccgcgatt
    11821 gcccttgata aaaggatgtt gtgaagtggc ttcgcgatct cgggccgaag gatcttcgga
    11881 atattgaata gcaagccgct cgaaaggctc gcctgaacag gcttttcgca atatttcatc
    11941 cattcttttc caggtagcaa gcgtgtcgga aggcgaagca tccttgtcgc aacgaagcag
    12001 gatatgactg gcccgcaaat cccattgcat acgttcgtaa gcttcattta ccagtttccg
    12061 gaaaacgaca gaatcgatga aatagggcgc cgataattgt ttcctatatc cagccagctc
    12121 gtctttcaaa acctttaccg tgtcgagtcc attaacttct gcatctctaa ctttcaattt
    12181 gaaattgata aaaagttcga gatattcttc catcgatttt ttatcagcta cttctgactg
    12241 aacattattc tttgaataga tattgagaaa ttcactcact ctgacgggtt gtccgtcaat
    12301 ggtcatcaaa acaggatcac tcgaattttg agcatgtgtt ttacaaatga tcaaggaaag
    12361 acctacaaaa actgcaataa acccaaaagt ttttcttctc atatatatac aattttattt
    12421 attgattttc ttcatgaaga ttaacggcta taattaggag cctcacttat aatagttaca
    12481 tcgtgaggat ggctttcgat gattccggct tgggtaatac ggatgaaggt agcctttcga
    12541 agttccgata tatttttgca accagtataa cccatacccg atctcatgcc tcccaccatt
    12601 tgagtaacca cttcattgag agaaccttta tagggaactc ttcccactat accttcggga
    12661 accaatttct taatatcgtc ttccatatcc tgaaaataac gatccttcga acctttctgc
    12721 atagcctcaa tggagcccat acctctgtaa gtcttatatt ttcttccctc gtaaattacg
    12781 gcttcgccgg gtgattcatc cacgcccgcg aacaaagaac ctgccatgat tgtggacgca
    12841 cctgctgcaa gggcctttac gatatctccc gtaaagcgaa tacctccgtc agcaatcaaa
    12901 ggcacagggg tatcttttat cacatcataa acctccatga tggcagtaag ttgaggaacg
    12961 cctattccgg ctataattct ggtcgtgcag atggaacccg gtccaattcc caccttgatg
    13021 gcatcaatat ctaacttcaa aaggtcttcg gctgcttggc gagttgcaat attacctacc
    13081 acaatgtcga tgtcagggaa agctgatttt acctgacgag ccatttctaa aacggcttta
    13141 gaatgaccat gagccgtatc gatgactaca gcatcgactc cttcggctac aagagcattc
    13201 actctttcga gtgtatctct agcaacacca acggcagcag ctacccgcaa cctgccttta
    13261 tcgtccttac aggcattagg cattgacttg agttttatga tgtctttgta ggtaattaag
    13321 ccaattaaat gtccttgttt gtctactacg ggtaattttt ctattttata ctgattcaaa
    13381 atctctgccg ctgtttcgaa gtcagtaaat tcttcggtgg tgatgacttt ctgggtcata
    13441 acctcactca ccgatttctg gatgttacgt tcgaaacgaa gatcgcggtt ggtaattatg
    13501 cccaccaaga tgagatcatc gtttaccact gggatgccgc cgattttatg ttcacgcata
    13561 atattgatgg catcggcaac cctggcattt acatttaccg taataggctg aagtatcatt
    13621 ccggcctcgg cgcgtttcac cttcctaacc tccaaggcct gctgttcgat ggtcatattc
    13681 ttatgaatca ctccaatacc gccttcgcga gcaatggcaa tggccattgt cgattcggta
    13741 accgtatcca tggcagccgt cacaatggga atctttacag caatattccg actgaaataa
    13801 gaggagatat ccacttcgcg tggtatcacc tccgaataag aaggaacaag aagaacgtca
    13861 tcgtaagtga gcccttcccg ctcgactctc gcagttcttt ctttcatagc tttctttttc
    13921 aattctgcaa aaataggaat tttaaagcat tgctcaccaa gcagtcatgt ttataaattc
    13981 cacactcctc gatactgcac tttccgacga gtatataggc tcagtcgagc aatacgaaag
    14041 ttcccgaccg atcaaaatat ttgccttttc gtgttaaaaa acggatataa tacacataaa
    14101 ccccactttc cacttttttc cccttacaag taccatccca gccttcatcg agcgagtggg
    14161 tttcgaaaat catctgccct attcgactga aaacagcaaa ataatattct gtgggatcga
    14221 tggccatgat aaaaggtttg aaaatatgac cgacagtgct gcgaaaaggg ataaaagcag
    14281 tgggaatata aaaatgataa tctggttcga tgactaccgt attggattgt gaaatctcct
    14341 tgaagaaaaa aggattgcca tgggcttcta tagcgcgtat aaaatacata gcacctgttt
    14401 cacgatcgat atttggatcg ctccatattg tgtcgtcggg atttagtttt gctaccaaaa
    14461 cagtgtctaa aaattctatc ctgtaaactt catacccttc gaggccatta taccaatctc
    14521 tatatgcatt ccactgcaaa tcggagctgt gcggattttt caacaatatg ttcgaagcta
    14581 gatttgatac taaggtctct tcgccacaac tatccaaaac aattaaacga taatgatact
    14641 ggtttttcga aacatttacc gcagaatcta taaaatatat aacagcatct ttttgcgtaa
    14701 actccttcac ttgtgcaaaa tttcccgaat aattggtcat tctttgaaga aagaattttt
    14761 gaataggagc cgcagtatct acatgaaacc cgagggcaat tgattgattg ttcaccacag
    14821 tgccataacg aatataagcg tattcgggtt gagggggtat tcttacatgt aagtaacgaa
    14881 cattactcga tgcggagatt tgtccgttga tcgaatttgc tctgataaaa aaagaatatt
    14941 ttaccttatc ttgaggctga ataaagtcat aagtcaaggt cgaagcattt agatggacga
    15001 aaggataaaa cgtgttgtta ttttcgctca gatacaccgt atacccgccg aggcctcccg
    15061 gcaggttggt ataagaagac caatgaagcc ggatgcgtcc taagcatact tcaaaaatag
    15121 gctcagccaa taaaatcgat ccctgggctt gggaaattcc catgtcctcg ctcacattgc
    15181 cacaataatc gaaggctgca acagaatatt tccatgattt tactgaagga tcattcagcg
    15241 tatcgataaa aaaagtggta tgagcatctg gaattaacgc cagtgtatcc caaatgccct
    15301 gatcttgcaa ataaacaatg taacccgctg catcgggagc attcacctct tgccagccta
    15361 aatgtatccg gccattcaca acacttacac tatcgaaaat gggtgtaggt ggctgttcga
    15421 tatcatgatc aacctttgca atagctgaaa ccgatccaca ataactatca tccacttcaa
    15481 tcttgtaata cctgtccacg cagggacgaa cctcatgtat ataactcaaa tcagttgtac
    15541 tgtctatttt tgtccacttt ccaccctctt cccggcaaaa aattctgtac cacccatcac
    15601 aactcgatgg caaagtatcc atcaaagcat tccattgcag ttgaatataa gtttggtcga
    15661 ggatagaacc aaccggtaaa atggctgcaa tcgtatccga atcaaacgaa aggcccgtag
    15721 tagtaatagt cctaacgaaa taataagcat gaacagcatt ggcatttgct ccaatatgtc
    15781 gataatgcaa tatttccaaa tccggaatct gagcaatgag gtcgaaagaa gaaaaaatat
    15841 cggatgaata gtaaacttca tttcgctgaa aatcaataac actcccccct ggctgccaat
    15901 aaatggtaac atcgccattc gctgccactt cactgcattt tatttctaca ggtaatgatt
    15961 gcacctgtga aaatggcatc gtatataaaa aaagaatgaa aagaatggaa ggaaaggtca
    16021 ttttcatgaa acgaaaacgt aaagttcgtg tcctatattt ttgcaaattt aaagatacag
    16081 caaattatcc tttagtttcc taccgaaata aaacttcgga aaggtcgaaa tgtagcatga
    16141 agcgagtgca attgtcctat gcctgtgcaa cgaatctatt attattcatt attcatcatt
    16201 taaaacaaat catttgaatc gaatttcgat gttatgcatt ctatttaaaa aatttttatg
    16261 aatattgcat tctaacaatc tgcttatgac tttaattgca ccttctattc tgactactga
    16321 tttttccaaa ttatcggata cgatttctat gttgcagctt agcgaagccg attggattca
    16381 tctcgacatc atggacgggg tatttgttcc caatcttact tttggttttc cagtaattcg
    16441 tgcaattagg aaacacacta ccaagccatt ggatgtgcat ttaatgatgg tagaacccga
    16501 gaaatatttc aaagaatttc gagaagccgg ggccgatatt ttaagtgtac acgtcgaagt
    16561 ttctccgcat ctgcatcgcc atctaactac tatcaagcaa ctgggaatga aagccggtgc
    16621 agtactcaat cctcacactt ctctagtaaa tattgaaaat gtacttcacc aagtagacat
    16681 cattctttta atgtcggtga atccaggttt tggtgggcaa gtattcattc ctcaaacaat
    16741 cgaaaaaatc agaaaactaa aaaaaatgct tctcgagtca ggctcgaagg cgttgatcga
    16801 agtggatgga ggcgtaggtt tggacaatgc tcagtccttg atcgatgcag gtgtcgatat
    16861 tttagtggta gggaacagca ttttttctca atccgatccc cagcttgcca tccaccaatt
    16921 gaaacattcg aaaagatgaa gaaacttctc atccttgggc tgattttaat ttgcctactc
    16981 gctcgtgcac aagagaatca gctcattgta atgttttaca ataccgaaaa cctcttcgat
    17041 accgaagatg atccggaaaa aacggatgaa ggctttaccc ccgaaggaaa ctatcattgg
    17101 acgaaagccc ggctatataa aaaattgaat aatctagccc gtgtgattct tgcttcaggt
    17161 gaatgggaca atccttcaat cattggtctt tgtgaagtgg aaaaccgaaa cgttttgcag
    17221 atgttcaccc aatatacggc tctcaaatcg gttggatatc gttttatcca ctacgaaagc
    17281 cctgatccgc gaggaatcga cgttgctcta ttatataatc ccgatgattt tatgcctttg
    17341 atttcaaggc ccatacgggt aaaaaacgaa aaaggagagc ttttgcagac cagagatatt
    17401 ctctatgtga aaggcttggt aagggatata gatactctgc atattttcgt gaatcactgg
    17461 ccatcgaaat atggaggcgt ggcaggttca ctccctcggc gctttgccgc cgcgcttaca
    17521 cttaaacaac aagtagattc tttgcttcat caaggcgaag ccaatatact gattatgggc
    17581 gatttgaatg ataatccttt cgacatttcg gtgaaagacg tgcttcaggc ctgcatagat
    17641 acagcaaact gcgaatcatc tcttatcaat ctgatggctc cattgatgaa aacgcaaaat
    17701 gcaggtagtc ataaatttca aggtcaatgg gatattattg ataatataat cgtttcgaaa
    17761 actttatacg aaggcaaatt gcacctgaga gcccgaaaag caaaaatatt tgctccccct
    17821 ttcatgctga caaaagacaa aaattatact gggaaaaaaa tctatcgtac ttataacggc
    17881 atgaaatacg aaggtggata tagcgatcac ttgcctgtca ttacctttct tgcggtaaaa
    17941 agacctgaga tataaatgat cgaataaata tttcatcaat aagaatcatg aaaaatctat
    18001 tctactcctg caaaatgacg cataaattgg acacgttcgt atgaagcagg attttctata
    18061 tttttcagac tcattttacc aataatctgt tcaagtgatt catatccacg ctggtccatc
    18121 caagaaccaa tttgctggtt agtatcggca atacgtgaaa aaccataacg atagagagcc
    18181 gacaccattt gtacggcttt tgcgccaacc agtaagagtt tcaaagcctc ttcgccacta
    18241 tccacacccg tcgaacccgc caaatcgcaa tgcattctgt cgctcagcat ggcaatccag
    18301 cgcaaaggga gattgagctc accaggttga ctgaacacat gcccgctggt tacttccaag
    18361 gtatctatat caatctcggg actgtaaaaa cgattgaaaa gtaccatccc tttgattcct
    18421 gtccatgata actgtaaaaa cgtcctggtc aaactcgaaa aataccagct cagcttaatg
    18481 cttacaggaa tattcagttc cctgagtaca gcctttgcta tatcgaaata aagtttctcg
    18541 ttttcttcac tactcctgaa agggtcggaa ggaaggataa atatatttaa ttcgagtgca
    18601 tcagcacccg cttgttcgat ctgccgggca aaactcgtcc actgagcagc agtaatacag
    18661 ttaatgcttg caatgatagg gaaagacgta ttttccttag catcaaaaat caatcgaaga
    18721 tatttatcga gttcatgcga ttgagtgtat tgatgaatat aatcccatgc ttcaggataa
    18781 ggcgattgct gaaaagaatg ggcctgatgc gcctcaaacc tgatttgttc ttcgaaaatg
    18841 gatttcaata caaccgcacc cgcgccctgc cgctcgatct cgacgatgtt ctttagagaa
    18901 gaggttaatc ctgaactacc agctataata gggcttctca aattcaagcc catataattc
    18961 gtattcaaat tggccatatc acgaaaattt taatcttgat ttttcgaacc acaaacctaa
    19021 ataattttcc cttcgtttgg atgatttcgg caaacttcat tctcaatgaa tgttcgaacc
    19081 attgaaatta tcattcctgc gaatccatca aataattcag acagacaatc gaattccctt
    19141 atctgatgag atttccgtgc tttctttttg acaagaagat tttcttgatc ccactcgact
    19201 aaatcgaaaa cataaggcaa caaactctta ttcaggaagg taaattcaca ttcaacacct
    19261 cgaagaacct ccacaaatac gtttcttttt caaacaacca ttcctttcga gaaatatgcc
    19321 atgaagcaga atttcaaagt tttcgtgctt cggcaaaact gtaataattg ttgattccaa
    19381 taataagatg atcgagaaga acaatatcga gcaacttacc tgcggcagca atcttttcgg
    19441 taagattaat atcggcggca cttggttgaa gattccctga gggatgatta tgtgcaatga
    19501 tgagagaaga agcaaacttt tcgagggcca accgcatgat gcgcttggga tcaactaccg
    19561 ttccatccgt ccctccttca cctatcaaaa tcggttcgcc taaaggactg ttggccttgt
    19621 tcaagaacaa tgcataaaat cgctcataat gattatcttc taaatatttc ctgaaaatca
    19681 gataagcatc cttgctcgac tgaattttcc cctgttcaaa ggcttcctcg gagagtcggc
    19741 gcttgccaag ctccaaagcg gcctttatgg taacggcctt tgccattcca attcctttat
    19801 atttcatcaa atccgataat tccagtctgg aaagagccgt taaattgttc tggtgatcct
    19861 gtaagatgcg ctttgccaaa tctaaagcac tctcttcaat gttacctgaa gatattaaaa
    19921 tagccagtaa ttctgcattg ctcaatgaat cgggtccctt tttcagcatc ttttcccgag
    19981 gccggtcgtc ctctgcccat tgatgaatac tcttactctc ttccttcttt ttcataagat
    20041 tttcgttttg ccgaagtaaa attaaatact ttactcgatt cctatggtta catctttagg
    20101 ggattaaaaa tttccaaaat cctcatcata ctgaaatctc cgtctataaa aattttcagc
    20161 ctgctcgaat tgtgcattca tatcaatccc aagctacaaa tcctcacatt agctccagaa
    20221 tcaaaatgaa ttgcattctt ttatctttct atttatttga tacttcagaa tcccagatgg
    20281 attcaaatca tcgaaaactt ttcaaatttt aggactcttc cccatgataa attctatctg
    20341 agctccatct ttcaaatcag aatgatgaat aaatggggtt gaaaggggtt tgccgttgag
    20401 gagaacctgc tgtacataag gtatatcaga ccgaaaattt ttcgtaacga tatgaatatt
    20461 atgaccattc tcaagctgga agttaatttc ctcaaaagca ggcctgccta tggtatattc
    20521 ggtgctaccc gggcaaatcg gataaaaacc caatgaacta aagatatacc aggccgacat
    20581 ctgaccgcaa tcatcattgc cacaaaggcc tgatggagaa tttctataga atgttttcaa
    20641 aattttttcg acctgcaatc ctgttttcca aggatgcgaa gtgtaattat acaaataagg
    20701 aatatggtgc ccgggttcat ttccatggac ataatttccc attaccccat ctttagtaat
    20761 atcttcagta ttttcgtaat attcttcggg caacgacatg gtgaacaacg tatcgagata
    20821 ggcttcgagt ttatttgcgc cgcccattag ctggattaag ccatcgatat catgaggtac
    20881 aaagaggctg taagtccagg cattcccctc tataaatccc tgattatgcg tcgacagagg
    20941 atcgaaaggt ttcacgaagt gtccattacg ttttttaggg cgcatcattc cggtttcata
    21001 gtcgaataca ttacggtaat tcaaggaacg attcttgaag atttttgcca gactatcctt
    21061 gccgagggct aaggccattt gtgaaagtgt ccagtcgtcg taagcatatt caagcgtcgt
    21121 tgacaccgaa gttggggatg tttcagaggc aacatatccc aactgcatat attcacccaa
    21181 tccatcgtaa gatttgtagg ttgccgtttg cagcatatcc tttaaggcct ggtcgggcgg
    21241 aaaatctcta atgcccttta cccaggcatc ggcaacgact ggtaaggcat ggtaacctgt
    21301 catacaccaa ttttcgttgg catgatgcga ccaaacggga agcatgccat gcacactctg
    21361 gctggcatga gcaagcatgg agcgaatcat gtcgttggct tgtgatggat gatagagcaa
    21421 aaggaaagga tgcaaggcac gaaaagtatc ccataatgaa aaaaccgtat aattggtaaa
    21481 tccttgggta gtatgaatct catgatcgag gccacgatac cgagcatcta catcctgata
    21541 aatatttgga cacaataaac tgtgatacag cgcagtgtaa aacacaattt tttcctcatt
    21601 cgtagctacg atcttaactc tgttcaattc agcatcccag ctctgtgttg cctgctttcg
    21661 aatctcatcg aaggtaaaag gagctgcttc ggtaagaaga tttttcatgg caccttgctc
    21721 actcactcct gaaataccca tttttacaac caattgttca cccgatcgaa tatcgaactt
    21781 aaaaatggcc tttaatttcc gaccgctacg ttctggaaaa ttatggtcca ttttccatct
    21841 gcgccaaaaa cctgtataga tttccttatc aagattgagc aaattatatt cctgaacggg
    21901 tttagaaaat gtcatggcaa aatagataaa cctctcacgg gcccatccgt tggtttgacg
    21961 ataacccgta accagtgtat cattgactac atgtaatgag ctccacacga ctttgccgtc
    22021 gtaattataa attccatgcg tcatatcgag taaaaggtag cagctgtcgg cacgatgaaa
    22081 agtgtaacga tgtacgccag tgcgctgggt agccgtaagt tccgcatcta tgtcataatc
    22141 ggtaagacaa acccggtaat aacccgcttc ggccttttca cgacttttgt cgtatcggct
    22201 tctatagcca ctccctggtt ttgaagctat accgggattg atttttatct tgcctgaaac
    22261 aggcatcaat aaaaaatcgc ccaaatcgga atgacccgta ccatgcaaat gagtatgact
    22321 aaaccccaca atggtcggat cgttatactg ataacctgca caatactgat aaacctgagg
    22381 attatagaca ttcccatcat tataaggaat tgtatcggta tcgggactca actgcacaaa
    22441 tccaaatgga accgtagccc ctggaaaagt atgccccatt ttgtcggttc caataaatgg
    22501 attcacatat ttcgaatttc cctgaccaaa ggtccaacta aaagccaata aattgataat
    22561 gagtaaaaat tttttcatcg aaaatcttta aaggtttaga tataaaacag cacgaaatta
    22621 cggaaaagtc tctaagggtt ttgaattcct ccataaatgc atctcgaatt cttttcggtt
    22681 atccatagtt ccgtgcgcga attagcctta tctttgcaac aaagggatga aataatgagt
    22741 tcaattatag aatcagcaag ttataatgaa gaaagtattc gctcactcga ctggcgtgaa
    22801 catattcgca tgcggcccgg aatgtatatt ggaaagctgg gtgatggggc ttcacacgac
    22861 gacggcatct atgttctcct gaaagaaatc atggacaacg ccattgatga attcgtgatg
    22921 ggatttggca aaaccataga agtaaacatt caggatcggc gagtgaatgt aagagactat
    22981 ggcaggggta ttcccctggg acgcctgctt gaatgtgtct cgaaaatcaa taccggcgca
    23041 aaatatgatt cgaaggtttt taaaaagtcg gtaggactga acggtgttgg gagtaaagcc
    23101 gtcaatgcct tgtcatcatg gtttcagatt caaagcattc gcgatggaaa gacaaagatt
    23161 gttgaatttc ataaaggatt gctgataaaa gagatagaac aatcctctga tttacgttca
    23221 ggaactcttg ttagttttat acccgatgaa gaaatcttcg gtaattatca ttttctcaac
    23281 gaacatgtgg aacgacagct atggaactat gcttttttga ataacgggct taccattctc
    23341 tttaatggcc agcgacttca ggcaaaaaac ggattggccg atttgctcga agtaaacatg
    23401 gaaaccgaac ctctttatcc catcatccat ctgaaagatg ctgattttga atgcgctttc
    23461 acacatgatt cctcacatta tggagaagag tattattcct ttgtaaacgg gcagcatacc
    23521 attcaggggg gaacgcacca gcaggctttt agagaagctt atgtaaaaac catccgagat
    23581 ttcttcaaga aagattatga tacctccgat attcgcactg gtcttattgc cgctattagc
    23641 attaaaattc aggaacccat cttcgaatct caaaccaaga cacgcctcgg ttctaccctt
    23701 atggaacccg aaggacaaag cgtcagcaag tatattcatg attttctaaa aaaacatctc
    23761 gacgattatc ttcatatcaa tcctgctacg gccgaagcca ttcagcgtaa aattctgcaa
    23821 agtgagaagg aacgcaagga aatggcaggc attaagaagc ttgctcgcga aagtgccaag
    23881 aaagttagtc ttcacaataa aaaattacgc gattgtcgta ttcattacaa ttcgaacgac
    23941 cctcgcaaac tcgaatccac tctttttatc accgaaggag attcagcaag tggttcgatc
    24001 actaaaagcc gcgacgtcaa tactcaggcc gtattcagcc tgaaaggcaa acccttgaac
    24061 agctatgggc tttcaaaaaa aatcgtttac gaaaacgaag aatttaacct cttgcaatca
    24121 gccctcgata tcgaagatgg tttggaaaat ctacgttaca atcagattgt gattgccacc
    24181 gatgccgacg tggatggcat gcacatccga ctgctcctgc tcacattctt tcttcagttt
    24241 ttccccgatc tggtaaaagc cggacattta ttcattttgc aaactccttt gttcagggta
    24301 agaaacaaac aacatacatt ttattgttac aatgaagaag aaaaggaaga agccttggct
    24361 aaaacgggaa agaatcctga aatcaccaga ttcaaaggct tgggagaaat ttctcccgaa
    24421 gaatttcgtc attttattgg cccacaaatg cgcctcgacc ccgtaattct tcgcaaagac
    24481 caagctatca acgaaattct cgcatattac atgggaaaaa atactcccca acgccaggca
    24541 ttcatcatag aaaacctacg ctacgaggcc gatgctattt tcatagattg attcatatcc
    24601 gttcatgagt attttccgaa cagatcaaga aaaatcaatt tctttatatc gaaatttcta
    24661 ttttgttcga aaaatcattc ggacaatttt taaaaaataa aatttactat aattttgtac
    24721 tacattgcct gatattatca aataatcata tatctcattc actgtcaaac atttaaaaat
    24781 ataaaaatta tggatagaga aaaattcaag gaaaatgcaa aaaagaacat agacgatctc
    24841 tttgccaaga tcgacgaaat ggaagccaaa aaggacaagg ttaaagctga aactcgagcc
    24901 gaatacgaag ctaagatcaa cgaattgaag gcaaaaaaag acgagcttca aaaaaaatat
    24961 gaagcactaa gcgaagcaac cgacgaaaaa tgggatgagg ttaaagatac cttttcatcg
    25021 gccatggatt cgtttaagga gggcttctcc aaaatcgctt ctatttttaa atagaaaaat
    25081 agtcgaaact ttctccaaat tttaaggaaa atattggcca gttgccatat taaatagaga
    25141 atttaaccct ttcaaaagaa aaactcgaat atgaggggag caggttttat cgttttatat
    25201 actgctcttc atagtatttt tgatattctc cagaagttac atgagcaagc cattcaggat
    25261 tggagagata ccaatctatg gttttttcga ggccttcctc gaaagttacg ctgggtttcc
    25321 atcctaattg ctcctgcaac ttggatgagt cgatggcata gcgcaggtca tggccagctc
    25381 tgtctttaac aaaagcaatc agcttttccg aggttccgga aggcctttcc agcttacgat
    25441 ccattattct gcataaaagt cttatcaagt cgatgttctt ccattcatta tttccgccta
    25501 tgttataggt ttcgccatta ggtgctcgat gaaaaatcaa atcgatagct gccgcatggt
    25561 cttcaaccca taaccaatct cttatgtttt cgcctttccc ataaactggc aaggtcaaat
    25621 tgaaacgtat attattgatg cacagaggaa taagtttttc aggaaactga taagcgccat
    25681 aattattcga acaattcgaa ataaccgttg gcaaaccata agtatcgtgg taagctctca
    25741 caaaatgatc actgctggct ttgctggctg aataagggga atggggctca tagggagttt
    25801 cttcggtgaa aagtcctatt gaacctaaag aaccatagac ctcatcggtt gaaatatgat
    25861 aaaatcgttt gtcttcgaaa tttcctttcc agataaaacg tgcagcattc aatagattga
    25921 ccgtacccac cacattggtc atgacaaatt cgaggggatt ggagatagaa cggtctacat
    25981 gagactcggc ggccaaatgg atgaccccat cgaaccgatg aagcacaaat agtttcatta
    26041 tagcgtcatt gtcttgaata tccgccctga taaaggaata gttaggttta tcttcgatat
    26101 cgcgcagatt ttcgaggttt ccagcatagg taagtttatc gaggttaaaa atatgatatt
    26161 gaggatattt attcacaaaa agcctcacta catgcgaacc aataaaccca gctccacccg
    26221 taataagtat tttcttttcc atagccaaac aattcaataa taatgttatt catttaattt
    26281 tccggccagt cgaagttccc aacgccaggc tgagagagtc atttcgtcga gggaacgttt
    26341 ggccttccat cctaattcct gatttgcata agaagtatca gcccacactt tttccacatc
    26401 acctggccga cgatcggtaa aaacataatt caatctaaca cccgacactt tttcgaatga
    26461 ccgaatgact tctaacactg tatgtccctg ccctgtaccc agattaaaga tttcgtagtt
    26521 attcttcatt ttattttgga gcatacggtc gagggctacc acatgggctt cggaaagatc
    26581 gaccacgtga atatagtcgc gaatggccgt gccatcgggt gtattgtagt cttttccata
    26641 aacacgcaaa acttcacgtt ttccaatagc ggtttgcgtg ataaaaggca ccaaattact
    26701 gggaataccc aaaggtaatt ccccaatcag tgccgatgga tgggccccaa tgggattgaa
    26761 atatcggagc gaaatagcct ttaattgcgg gaatgcatgt atggtatcac gaatgatttc
    26821 ttccgagatt tgttttgtat tgccataagg agatatggcc tctttgatgg gcgaagtttc
    26881 gcgcacagga agttcatcgg gctggccata aactgtacag gaagaactga atactaaatt
    26941 agcaatccta ttatcgagca ttccttctaa aatattgatc agggattgaa gattgttgcg
    27001 atagtaaagc agtggttttt caactgattc tcccacagcc ttgtaagcgg cgaaatgaat
    27061 aattccagca atatcactgt gttctttgaa aagttggaag gtaggttctt gttcgctcag
    27121 gtctaattga taaaactcag gccgaatgcc agttatttcc tctatattat caagaacttc
    27181 aatccggcta ttggaaagat tgtcaactac aaccacatcg tatccttttt gttgcagttc
    27241 aaccactgta tgtgaaccaa tatatcccgt tccccccgtc actaaaatct tcataagtga
    27301 tgttcaatta agccaattca ttgcattcca tttataccac tcaaacattc ttcgaaaaat
    27361 acagatttta caaaattaca aaattcaact attcttttga cttcatgaaa ttattctgct
    27421 catcgcccaa ttctcaatat tctaatttta tgaatttaca gtttacaatt acaaaattac
    27481 aaatcccaaa ttccagtccc cttgtctcct cgtccccttg tctcctcgtc tcccttcctc
    27541 cattcacctt ttctccgttt tccgttttta actaacaact attaaactcc aaattacaaa
    27601 tcccaaatca caaacaaaca tcgacaaaat ccaactgata actgacaacc aacaacttac
    27661 aactaaaaaa ctccaaatta caagtttcaa atttcaacta aattataaat tcaagtctct
    27721 cagtcgccca gtctccaaat tccagtcacc atccaccatc caccatccac cattctcctt
    27781 tcctcccttc ctccttttta actttataaa cttttaactt tcaaatttta actcactaat
    27841 caccaaccac ccatcaatcc accttcagca aatatggatg aaagtcgcct gtaagtccaa
    27901 agggctttgc attacgaatt tgatagacaa tttccacact ctggcgtgca tcttctaaac
    27961 catagccccc acctgacaaa atatcctcat aaactcgagt atgtaaatcg gcaaaacctt
    28021 cactaaactc aaactcttca ccatctatta atattgaacg ataagtacgc tgacctttag
    28081 cggcaatttc tttaggcaaa tatttcgaat cgatactcaa gaaccatctc actctggctt
    28141 tatcgtactg taaaaaacca gcaacaactg taggtgacga atgatgaacg gccaccaatc
    28201 taatctttcc aaaaataaaa cccagcatat cgaaaaaatg aacgccaata ttggtggcca
    28261 aaccacccga tttttccaca tcacccttcc atgaataatg ataccattta ccccttgagg
    28321 taatataggt aagatcaaga tcatagattt tatcggtagg gccatttttg attttctctt
    28381 ttaatgcaat aatttgagga tgaagcctca attgaagaat attatagatt ctgaatccgg
    28441 tttcattttc aatgtcttct aaggcatcga tgttccaagg attcagcacg atgggttttt
    28501 cacagatagc attagcttga ttacgcagag ccagtcgtat gtgagaatcg tgcagaaaat
    28561 ttggagaaca aatgcttacg aaatctattt tcttttctcc ctgtcggcgc aatttatcca
    28621 aatgacggtc gaaacgctcg ggttgtaaaa agaaatcggc ttccgggaaa aaagaatcga
    28681 taatccccac gctgtcgttg ggatcgagag ctgctaccaa acgattgccc gtgtctttaa
    28741 tggctttcat gtgcctgggt gcaatgtaac cccctgcacc tatcaaggca aaattgtaaa
    28801 ttaaattttc cataaaatta aaaatttaag aaatcttgaa tacctgatta ttttctaatt
    28861 tatatttttc gtgactttca gggcaaatag caatattatc ttcgtcgaaa tgcaaacgat
    28921 gaccatattc actcatccat cctatttgtc gggctgggtt tccaattacg agggcataag
    28981 gtagtacttc gcgagtaaca acggctccag ctcccacaaa ggcatattct ccaatattgt
    29041 gtccacaaac aatagtagca ttagccccaa tggtagctcc tcgttttact atcgtttgag
    29101 cataatgtcc cctgcgattg acagcactcc ttggattgat gacattggta aaaaccatag
    29161 aaggacccaa aaaaacatca tcttcacaaa ttaccccagt ataaatggaa acattgtttt
    29221 gaaccttgac attgtttccc agaactacct tgggcgaaac cactacattt tgtccaaggt
    29281 tgcagttttc tccaattgta caacccgaca tgatatgcga aaaatgccag atgcgagtgc
    29341 cctggccaat cacacatcct ttgtcgatta cagcagtttc atgcgcaaaa taatctttgg
    29401 ccattttcga cagatttttt attattattt gacccgtgaa ataaattcta aaacctgatc
    29461 acaaatatat tgtaattgtt cttcatcgag ctcggtatgc atgggtaagg aaaaaacatt
    29521 ggccgagagc atctctgtta cagggaaatc gcccttgcca tagcgttcgc ttttataagc
    29581 cttttgtaaa tgaaggggga caggataata aaccatggca ggaatatcct tttttgccaa
    29641 ataattcact agatcatccc gagaaatacc attgcaaatc aaggtatatt ggtgaaaaac
    29701 atgcgatgat ttgggatggc gaaccggtaa cttcaggtag ggaatgtctg caaaagccct
    29761 ttcataatat tgagcggcct tttggcgggc tgaaatatat tcatcgagat ggggcaattt
    29821 aaccttcaag atagccgcct gaatgctgtc taagcgtgaa ttcactccaa caaaatcatg
    29881 ataataccgt gcaaacattc catgattggc tatacctctc agcatatttg caagctcgtc
    29941 atcatttgta aagattgccc ctccatcacc ataacaacca agatttttac ttgggaaaaa
    30001 agatgtgcat ccaatatttc ctattgtccc agccttcttt atactgccat ctgttgagtg
    30061 atattcggat cctatagcct gacaggcatc ttctattaca taaagatgat gacggcgggc
    30121 gatttccatg atggcatcca tatcggcaca ttgaccaaaa agatggacag gaacgatggc
    30181 ttttgtatgt ggagttaggg ctttttcgat agcttcggga attatattga aataatcagg
    30241 gtcaacatcc actaaaactg gcgtaagacc aagtaaagct attacttctg cggtggctac
    30301 aaaggtaaaa tcggccgtaa tgacctcatc atctggttta agaccaagag ccatcatgga
    30361 aatttgaagc gcatcggttc cgtttccaca gggaatcaca tgctttactc ccagatattc
    30421 ctcgagatct ttttgaaaaa attttactac agggccctta ataaacgccg tggaatctat
    30481 tacctcctga atggcctggt caatctccgg tttgatcttc acatactgag agtataaatc
    30541 caccatttgt atctttctca tgaatttcta attgttttta cattgcattg cgcaaaggta
    30601 gagaatttat acaacccagc gcgaagccaa tagaatatat atgcattatc atttgaaccg
    30661 gtactgctat ccgatacact cgatttattc ctgccccatt cgatcggatt caggaattcg
    30721 atgtcaagaa tgatatataa taaaattaaa atttcaacca ataaatcatt ctcgtgcatt
    30781 agatacgttt ttacttactt ttgcgtttca ttcgacatag gctgcttatt ttaatcttca
    30841 cagaaaaaaa gaagactgaa tgcaaaaaag gaaaactatt atttttttaa ttctcatcag
    30901 ctcgattttt acttttcaaa aagctgaagg acaagtaaat attgctgatt ctatgattcg
    30961 aatctttgga gtttcggccc gtttgggaag tcatcttcca gctgccgata tggctgagcg
    31021 ttttggaatc agctatacgg tagggcctgc atttacttat aaaacaaaca aaaactggat
    31081 atgggatgct ggcttcgatt atattttcgg aaacgacgtc aagataaagg attcgctatt
    31141 tcgacttatt tcgacctcgc atcaatttat catcgaccag gaaggcgtgc cggccgatgt
    31201 tgcagtccag gaaagagggt atcattttta tgtctcctcg ggaaaattat tccctatatg
    31261 ggggcctaat cctaattcgg gtttgattgt ggaatttggg atgggctatc ttcttcataa
    31321 ggtcaatatt tcggttctca ataattcggc acctcaactg agaggtgatt ataaaaaagg
    31381 ttatgataga ttgaccggtg gtttttcgtt gaatcagttc attggatatc aacatctaag
    31441 ttcgaaaagg atttataatt tttttgtagg tctcgaatta atcgaaggat ttacctcttc
    31501 gcttcgcgaa tacgatttcg accgtatggc tcctgccgag ggaaaccgct tcgatatgat
    31561 gataggcctc aaagcaggtt ggattttacc tatctattcg agagcaccca aagattatta
    31621 ttactactaa gcaatgcatt ctttctataa tgtcgcaatt tattcttatt catttttgat
    31681 taaggtttcc tcaattttca atcctaaggc aaaggaatgg attaagggtc gaaaaaatct
    31741 gttccaaagg cttcaagata aaatcgacag agacaaaccc ctggtttggt ttcactgtgc
    31801 ttctttgggc gaattcgagc aaggaaggcc acttatcgag gcttttcacc aatcctatcc
    31861 atcttttaaa atcttgataa cttttttttc accttcaggt tataatgttc gaaaaaatta
    31921 tataggagcc gattatattt tctatttgcc tgcggatact ccagtcaatg caaaaaaatt
    31981 catcgaaata ataaaaccca aattggtcat ttttattaaa tatgaattct ggttaaatta
    32041 tctaaaagaa atacatgctc gtgatatcgc tctcttgctg gtttccggaa ttttcagacc
    32101 tcaacaggtt ttcttcaaat ggtatggaaa aatcttcaga caacaattaa aggcattttc
    32161 acatttttat gttcaaaacg agacttctgc gcaattatta aaaaatatcg gattcgacaa
    32221 tgtaagtgca tcgggtgata cccgtttcga ccgtgttgaa accattgctg ccaattctca
    32281 gcctctgcac gagattaaaa cattttgtgc cgataaaaaa gtgatcattg gtggtagtgt
    32341 ttggcctgcc gacttgaaaa ttttagaacc ttttatccag caagccagtg aaaattttaa
    32401 atttatacta gctccccatg aagtaaatcg agatattata tcgggattgc ttgaacatta
    32461 tggagataag gcaatcactt atactgctct caagaataga ccacaaaacg atgaaaaaat
    32521 ccttatcatc gatatagtgg gaatactttc ttcggtgtat cagtttggct gggttgccta
    32581 tattggagga ggatttggtg ttggaattca caatattctg gaagctgctg tttatggaat
    32641 tcctgttttc tttggacccc actatcgaaa atttcaggaa gcatgcgatt taatcgaaag
    32701 aggcggagca ttccccataa attcttctga tgatctattg aggattattt taaatgcaga
    32761 tttcgagaaa aataggaata aagtcgctaa aatatgcaaa gaatatgttt tacaaaatac
    32821 aggagccaca aaaattgtaa tgcaaggaat aaataaaata ataaaaaata aaggatagaa
    32881 aacattttaa attgttgaat tattttgaca ctattatagt aaacacataa aaatttatac
    32941 gggaaccatt tataaattaa ttgtttatca ataggagtgt atatattgca gaaaggataa
    33001 agatatttac gggtttgaat gacgattcca ttacctcttg aaatatcatt atgtcttcaa
    33061 aataaggtta atttataaat aaaatttctc caaacaaata tattgattct caatgatatt
    33121 attatacaga gttttttagt tagaacgatt aagagattga aaatgatcga acaaaatggt
    33181 tctttatatt aaatatctga atatatctga tgttaaaaac tcagccattt tcaacccctt
    33241 cagcaaaata ccctaaagct ccaattgcgt actccttttc tcctcaaaat aaattacttt
    33301 tgcaaaattc tttcattata aacagtcatg ggaaaacttt cgcataccga tgacaagggc
    33361 aaagctctta tggtggacat tagtcacaag cctttggaga aacgcatagc cattgcgaca
    33421 ggccaaatct tcctgcagaa ttctaccatt caattaatca aggaaaatct gataaaaaaa
    33481 ggcgatgtat tgactacggc cgagatagca ggcattcagg cagcaaaaca aactggcatg
    33541 cttatccccc tgtgccaccc tttgcctctt tcggacgtcg aggtggcgac aagaattcat
    33601 gaacaatcgg tcgaggtaac atcgaaagtt ataaccatct ggcaaactgg agtcgaaatg
    33661 gaagctctca cggctgtaag tatagccttg ctcaccatat acgacatgtg taaggcagtg
    33721 gacaaaacaa tgtgtatggg aaatattcac ttggtggaaa aaacaaaatc atcccccgat
    33781 gaaaaagagg cctaagttga aaaaatcgaa attttatttc attttatttt ttagttgggg
    33841 tttcatgata tcttcacaag cacaaatcaa cattccccat ttcatctttt cggggatgca
    33901 tcatctttcg gaagggagat ttgtagatgc catcaaaagt ttgaatgtag tcatcaatgc
    33961 ggctcctgac atgttcgaac catattttta taggggtatt gcaaaatata atcttggcga
    34021 ttatcaggga gcggaacagg attttactcg cagcatagaa atcaacccta tgttcagcga
    34081 agcttatcat tacagggcta ttacgcgcaa caggcttcgc aattatcagg ctgctatgga
    34141 tgacttcaag gaagctctcc gaatagcgcc tcttagcgca gaaatttatg taaaccgaag
    34201 catcaccaga atgggtttga aagactataa aaatgctttg atagatgtcg attcggccat
    34261 caaaatcaaa aacgacatcc ctctggctta tcttgcccgc ggtcttatca aacatcaact
    34321 caacgacact ctgggagcaa tcgccgacat caacaaggct attcaactca attacttctt
    34381 taccgaagct tatatacgaa gaggaagcat taaatacgat attggagatt ataagggagc
    34441 tatagatgac ttcactcagg ctatcaaaat ggaacccgac aatcctgcag tttattttca
    34501 aagagccatt gcatggatta aactcgaaaa taaaaatgcc gcctttgacg atctgaataa
    34561 agttctcgag ctcgatccat ataatgcact tacttattac aatcgagcca tcatcaaagg
    34621 agaattgggc gatttaaaag gagcattgga ggattacaac cacgtacttg cactaaatcc
    34681 cgacaatatt ctggcagctt acaaccgcgc cattactcgg catcaactcg aagattatca
    34741 tggagccatc gaagattata ctaaagctat caaccttttc cccgatttct cgaaagccta
    34801 ttttatgcgt gccgaggcca agcgaatgct caaagatgaa aaaggagcct tcgaagatcg
    34861 caataaagcc atacaaattg aagaaaccat cgctaaaacc aatgactcca tacttcgact
    34921 ctacaccgat tctacctatt tagccagcat catcgaattc gaagccgatt ttagaaaaac
    34981 ttccgccgtc gatggaagaa ttcaatatca acctgtttat gtcgatctcg aacagaattt
    35041 catcgtaact tatctttaca acgactccct atatgtagaa caaaaacgag gcgaatactg
    35101 gctcaaaaaa atcgacgaca tcggtaaaaa agtggattat aaactcagct ttggccttac
    35161 caacaaagaa accaagctta cacccgaaca aattgcactt tatctgaaaa atatcgacct
    35221 gagcacaaaa ctcaacccat ccgatccgct tgcttacttt tttatgggta tattaaatgg
    35281 catgattaaa aattaccaaa gtgctctcga tgcctataat aaggccctgg aactcgatcc
    35341 cggtttttca ctggcctatt ttaatcgtgc caatactcgt ttagagatgc tcgaagaact
    35401 gaaggaacaa gaacctctac ctgtggcaat ttccttcgga aacaacatgc agcagcctcg
    35461 tattacagaa aaaaccccac taccagaata tcaggaagtt atcaacgatt ataacaaagt
    35521 gatcgaattg aaccccgact ttagctttgc atggttcaac cgagctaaca taaaagcaag
    35581 aattgcagat tatgaaagcg cactccagga ttattccata gccatccaac tggaacccaa
    35641 tctggctcag gcttactata atagagctct catcctcatc tatcaacata acaaccaacc
    35701 tgcctgccaa gaccttagtc gtgccggaga acttggaatt gccaatgcct ataatctaat
    35761 caaacgttat tgctcactca aaaattaaaa ctttcagaaa aaataaaaaa caaatcgatt
    35821 ctttattttt caattcatta ctaaaactaa atccattctt tgttattcat cactacaaaa
    35881 taacttatca tttaacatta tttataaaat gaaagtttac aaatttttat taatgttaaa
    35941 agaagacgat ttgtatgaaa aaaattagta ttgatgactg ttttgctggg gattaactta
    36001 aagagaaccg aatacctgaa aatatcatga tttatatata caaataaatt ggatacaaat
    36061 cattttttta tttttttgaa ttatgacgga tcagccggcc aataatataa ttgtaatcat
    36121 aggtggagga gctgcaggca tgatggcagc tataattgca gcgcgcaagg gagcacaggt
    36181 aattctttta gaaaaaatga ataaacctgg tcgaaaattg agtattaccg gaaaaggtcg
    36241 atgtaatatc actaataccg attcattgag agaatttctt acacatatcg accccgatcc
    36301 aaatttttta ttcaatattt tttcacgatt tttctcgaag gatattatcg attttttcca
    36361 gcaactcgga atcaatacaa tagaagaacg tggtggaaga atatttcccg aaaatgtatc
    36421 ggccatggag attgtaaaca ccttggtaaa ggaaatgcag cgtttaggtg tgaaaattat
    36481 ccttggagca cctgtcaaac agattatatg taccaataga aatataaagg gagtaagact
    36541 cgaaaatgga acctttatcc aatcgcataa aattattctt gcaaccggag gaagctccta
    36601 tcccgctaca ggttcgactg gagatggtta caaattggcc gccgaattag gtcatcatat
    36661 acgcacacca gttcctgcgc tcgtcccaat agatgtagct ggcagggttg ctcctactct
    36721 tcgaggactt actctgaaaa atataaaagc aactgtttat aataatggca aaataatagc
    36781 cgatgaattt ggtgaaatgc tttttacaca ttttggactt tctgggccta ttatattgag
    36841 cctcagtaga aaattaaacc gtatagattt taaaaacaac aaagtagata ttagcctcga
    36901 tctaaaacct gccctcgacg aaaaaactct cgatcaccgc ctgctacgcg attttaccca
    36961 acatggcaaa atgcaagcca aaacaatatt aaaaggatta ttaccttcga gcttgattcc
    37021 cgtatgcctc gatttgctac agatagaagc taaaaaaccc gctcatcaaa ttacggcaga
    37081 agaacgtaaa aaattaagat tatggcttaa ggattttcga ttccaaatca caggaattag
    37141 aggtttcaag gaagcaatca tcacttctgg aggtgtttca acactcgaaa taaatcccaa
    37201 aacattggag tcgaaacttg tctcggggct ttt
//
LOCUS       DAOORU010000008        34469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_1454_length_34469_cov_6.405387, whole genome shotgun sequence.
ACCESSION   DAOORU010000008 DAOORU010000000
VERSION     DAOORU010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 34469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34469
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1454_length_34469_cov_6.405387"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            697..2091
                     /gene="fumC"
                     /locus_tag="PLF20_01345"
     CDS             697..2091
                     /gene="fumC"
                     /locus_tag="PLF20_01345"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HOX79688.1"
                     /translation="MKTRTEHDTMGPVEVPADKYWGAQTERSRQNFRIGPEGSMPKPI
                     IEAFAILKKAAALANYELGVLPEEKCKLIVTVCDEILDGKLDDQFPLVIWQTGSGTQS
                     NMNLNEVIANRAEVLRGGTLGTGKTFIHPNDDVNKSQSSNDTFPTAMSIAAYKTLVEI
                     TLPGVKKLRDTLRVKANEFKHIVKTGRTHLMDATPLTLGQEFSGYAAQLDYAIENIEA
                     AMPHVSELALGGTAVGTGLNAPKDYDVKVAANIAELTGYPFKTAPNKFEALSAHDALV
                     EASGALKTLAVSLMHIANNIRLSASGPRCGIGELQLPENEPGSSIMPGKVNPTQNEAL
                     TMVCCQVIGNDMAIAVAGTHGHFQLNVFKPVIIANFLQSATLLGQACVSFNDHCAIGI
                     EPNLANIKKNLENSLMLVTALNPHIGYENAAKIAKKAHKEGLTLKQAALELGLVSAEN
                     FDKWVDPSKMTGEL"
     gene            2137..3234
                     /locus_tag="PLF20_01350"
     CDS             2137..3234
                     /locus_tag="PLF20_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HOX79689.1"
                     /translation="MECKELEIIPLRQWIHLDGFLLIAGPCSAESENQLLETAQALRS
                     SGKVQIFRAGIWKPRTHPAGFEGKGEEALKWLRNVKQLMDFMTAVEVGSPRHVELALE
                     NQVDVLWIGARTTVNPFMVSEIAQAVKGSDVAVMVKNPVNPDLDSWVGAIERIHQAGI
                     KKLAAIHRGFFFFHKTPWRNAPMWEIPIELKRICPCLPIIVDPSHICGKTDLIRDISQ
                     KALDLEMDGLMIEVHIHPEQALTDGEQQLNPIEFDDLIKHLIIRQKINNLDDDTLERL
                     RLEVDELDQELIEILARRMAMIDEIGHYKNQNHLTILQIKRWQQMLTDRLESGSQLGL
                     DKEFMHRLFQLVHDESIRRQQRIFNSNKSKE"
     gene            3394..3999
                     /locus_tag="PLF20_01355"
     CDS             3394..3999
                     /locus_tag="PLF20_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX79690.1"
                     /translation="MVLTKYTDKEIIEGCIAGKRYFQNLLYEKYKRSLFSVCLRYAVN
                     KQEAEDILQESFCKIFSRLGDYKAHGSFEGWLKRIVVNYTLSYLKQNKKDPLHFNNAE
                     VVENIADDEEENDNDLFKNISPQHCQTILKFIQELPVGYRTVFNLYAFEGYSHQQIAQ
                     TLQISENTSKSQLSKARRHLKNRIHKWMEKGITTNNPSSSY"
     gene            3999..5300
                     /locus_tag="PLF20_01360"
     CDS             3999..5300
                     /locus_tag="PLF20_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79691.1"
                     /translation="MEEKHNHIDTYIRQSLDGFEPEPSERVWKGIKRKLAWNEFVHFN
                     ITNFTSNIYQISATVLIGAGIIVGLVTQLLLVDKIRQQAQVPLAEKDIPTIIQPESPK
                     PEEKLFDSSSPIRSELSEKPLPSSISFLPSSPSFAPEPTIESLETPGLETSIAKKTAF
                     AIPLLPSHGIDRISLALPPSQLYQPLQKPGDSIAFGFEEPEKYPYRFGLEAGWWYNQM
                     TLPYPGKDFSYYWNAVGVAFEVEHRNFYAKTGLNFSRMFDRLPFDISYKSLDSIRYVY
                     NVHYYVPDPSHPDNIILITSRETLYDSITHNQNIFTTGTYNYLNIPFQLGCKFLKTGS
                     FAFDAYAGCGLQFLISKKEPLPVIFNYMKTGLNLNPSLVDHYNFNYFFFGGLNIRYFI
                     NENLKLELQPVYQYYMKSLGDHEGAGNPYSWGLKIGIGYYF"
     gene            5386..7935
                     /locus_tag="PLF20_01365"
     CDS             5386..7935
                     /locus_tag="PLF20_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOX79692.1"
                     /translation="MYNSKVYIRLIKVAAFLLPYMALIVSNAERLQAQEGPRSECQAS
                     FSYTYDSMNFLGIYFIDQSTGSFGSWQWDFGDGWGSSEQNPFHAYTQAGQYHVCLTIS
                     AEGKVCYDVECKDVRVPRPSNCQSTFTWAADSANPFFIRFTCHPGEDADDYFWSFGDG
                     DTSSLCNPCHVFADTGVYIVTLISSNRLNPSFCYSVYIDTVHIKIEPCISSFFAHPSL
                     FNPLEVQFIATPSGNIDHYYWDFGDGRTSMEPNPIYIYSDTGIFTVCLTVWNGNYMSY
                     CLDQSCQEVKIRISKCQADFSWLQDTIYPLKFRFINQSYGVLNDFLWDFGDGDTSHQI
                     NPFHAFPSPGDYHVKLRVRNFMHPEFCTDSKEKIVATGDLECIADFSWKIDSLKPLGV
                     IFTSLISGSPDYIHWDFGDGTTDSTLHPFHFFPDTGIYRVSLVVRNDFYPEYCADTNV
                     QQVHVYLKHHPKTDFTFVFDSLSLTPNLFHFYGQATSYQLQSWLWTFGDGTSSNLQNP
                     VHIFPQVQTFNVCLKITDFLPPKYYLTDKCCKTISTHEYFNLGGSVYDGNLPINNPQP
                     KGDTALVTLYKIYPGQMIIPWRSGTFHNLGYYWFTSLLEGEYLIKAQLTPKSVQANKF
                     FPTWAPQTLLWQSSNPVLLNENIFNANVHLVQRPYFEQGPGSVYGMVIVTSDPLLPTG
                     NPASNICVFLLDDNHQFLDFTLSDSLGRFHFDKLPWGTYVFIPDATGMSYTTDTAIVN
                     AQKPQVDDILLKMWNTDGMSAPPIEDTPLLSIWPNPASDGIKLKIRLTKPCGVNIELI
                     SATGQILNSHFWHASPSSNEFYLNLQGFSQGIYLLRVITPLQSHTYKILKR"
     gene            8039..8812
                     /locus_tag="PLF20_01370"
     CDS             8039..8812
                     /locus_tag="PLF20_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOX79693.1"
                     /translation="MAILGIDVGGSGIKGALVENGQVITERLRIPTPQPSTPEAIAVT
                     LNEIMKAFNYKGAIGCGFPALILDGVIKTAANIHKSWIDVPAEKLFTKTTGQPVFLIN
                     DADAAGLAEINYGAGKDKMGTIFVITIGTGLGSAIFINGQLVPNTELGHLKMYGKSAE
                     KYCSDAVRTKKNLSWKQWAMRFNEYLAYIEFLFSPQLIILGGGASKKFDQFAPYLKSR
                     TEIVPAELKNHAGIIGAAVWAHSKSQSQTISLIHQDNLL"
     gene            8840..12232
                     /gene="mfd"
                     /locus_tag="PLF20_01375"
     CDS             8840..12232
                     /gene="mfd"
                     /locus_tag="PLF20_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOX79694.1"
                     /translation="MRIPEVVDLYLNHPGVEAICSHLKDSDLPRIHLSGAKGSSLSLI
                     TAAIFKLTFQIHLLIAEDKERAAYLLNDIENLLGDRDKPFEKRRVLFFPTSWRKPYEI
                     EQTDNANVLARAEALNKIMTWGRNLIIVSYPEALAEKVVTKKSLKKNTFTIHAGEAVS
                     LDFFTDMLIEYGFERVDFVVEPGQFATRGGIVDVFTFSHEYPCRIEFDGDITESIRTF
                     DPASQLSIQKISRIDLLPNTEELMLSEKRENLLEYIPQNAMIWIENLEFTSDIIEREF
                     NKVSEIYISLKGPLAHRAPEDLYTVPDEFTASLLKHPLIFTGLNALPGSKPVAFNTIS
                     QSNFNKNFELLLSDLRIYDQQNYSLFIFSDNSRQLERIRQIIGDLSYSQKLPPVIFQG
                     VNYSLHEGFIDHQIRVVCYTDHQIFDRYHRYRLREGFAGKEALTLKELNNLKPGDYVT
                     HIDHGIGRFDGLEIIENNGHKQEVIRIFYQNNDILYVSIHSLHRIAKYTGRDGTPPTL
                     SRLGSNAWNNLKQRTKQKVKDIARDLIVLYAKRKASAGFEFSPDTYLQNELEASFIYE
                     DTPDQYKATVDIKVDMEKSIPMDRLICGDVGFGKTEVAIRASFKAVADSKQVAVLVPT
                     TILALQHYRTFNERLKDFPCRVEYLNRFKSNTEQKKLLAELEKGRIDIIIGTHRILSK
                     DVKFKDLGLVIIDEEQKFGVAAKEKLKALRINIDTLTLTATPIPRTLQFSLLGARDLS
                     VINTPPPNRQPVQTEIRSFSEETIRDAIYYELRRGGQVFFVHNRIQNIMEVAGMLSRF
                     VPDARIAVAHGQMEGHKLEEIMLDFIEGDYDVLVSTTIIESGLDIPNVNTIIINDAQH
                     FGLSELHQLRGRVGRSNKKAFCYLLAPPLASLSDDARRRLRAIEEFSDLGSGFNIAMR
                     DLDIRGAGNLLGAEQSGFIADLGYETYQKILDEAMQELRKTEYSDLFPSEESNTYVNE
                     CTFETDLEMRIPVEYIGNITERLALYKELDGMENEDDLQEFALRLEDRFGPLPAPALD
                     LLDSIRLRKMGKSLGMEKIVLKNGQMVCYFITDPNSPFYKSNDFTHVLQYIQTHQRIC
                     QLKENKNKLLLTIHHVDMVQKAIQILSEFHEKEKNEIREALK"
     gene            12853..14124
                     /locus_tag="PLF20_01380"
     CDS             12853..14124
                     /locus_tag="PLF20_01380"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HOX79695.1"
                     /translation="MKTKKWIILLSLLFMGIIRVYSQDIEYARFIVKTLTSSEYKGRG
                     YIGKGDKKAAEFIVGQFEKYRLQPFGKDYLQKYSFSINTFPEKMEVVVDDRKLMSGKD
                     YVISLASKGVKASYELEWLIDDSIPESSRWKELCSIDLTHKLVVTDEFHKNLVEVNLL
                     GAAGYVFLKDSNEIISWKVSNGRILKNFLVMDIQKEKLGKTTQRISVEFKNKYEENYQ
                     TQNVIGMIRGLGSPDTFLVISAHYDHLGMMGEKTMYPGANDNASGVGMMLDLARHFSL
                     PGNEPYYSLVFIAFSGEEAGLMGSNYYAEHPLFPLAQIKFLINLDMVGSGSEGIGVVN
                     GQHLKKAGLLLQQLNDKGKYLSEVALRGESKNSDHFPFYNKGVSCFFIYTMGKEWPYY
                     HTPEDTCCPSFTAYIGLFDLLNEFIVKVRFL"
     gene            complement(14386..15282)
                     /gene="gldN"
                     /locus_tag="PLF20_01385"
     CDS             complement(14386..15282)
                     /gene="gldN"
                     /locus_tag="PLF20_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HOX79696.1"
                     /translation="MLKMYRKIAVVILGLSLYCWGSEAQVIEPPKVKPMERPWPKIHT
                     PNRKRPVPYAPVREADVIWAKRIWRVIDLREKQNLPLYYPLRPVRDRKSLASVLFEAV
                     ITPQQENPYGSNYLTAYTDEDLAERYTTVDDVKRRLSFEYRDTYIDSLGNETTQEKVY
                     ELKAEDVVKYFIIEDWFFDKRRSQLDVRIHAICPVYYRFIMSNGQKQYLEDPSPTFWI
                     YYPEARFWLVNNEAYNPKNDAEWRTYDDIFTKRKFASYIYKEENVYDRMISDYASGLD
                     ALLESERIKSEILQFEQDLWEY"
     gene            complement(15289..16905)
                     /gene="gldM"
                     /locus_tag="PLF20_01390"
     CDS             complement(15289..16905)
                     /gene="gldM"
                     /locus_tag="PLF20_01390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HOX79697.1"
                     /translation="MAGYKETPRQKMIAMMYLVLTAMLALNVSKDIIQAFVTVNESME
                     QTNNSFDKKLRETYSKFESQATIGGASAKEWYDKALIAKKLSDSLVNYLNHMKNELIV
                     FSDRSIKTLEQADTTPLIRIKAKDNFDDPTRFFLGTDVSKGKAEDLKRVFKDYKEKMT
                     QLVKDEDHPKLQLGLITEGKFRDEYGAVQTWEKHYFYHTILSADLVILNKYIAEVRNA
                     EFDVVSLLSSYIGATDFKFNKIAAKVIPNRKYLFKGEEYQAEVLVAAYDTTSTPNIIY
                     RLGIDKWLPSYASGASNVTSGELGMAHLKISTAGMDYGPKKFAGVITILNPLGVEEQY
                     EFADEFVVQEPVAVISLDKMSVLYIGLDNPITVSVPGIPPDNLDIKISGPAEVKRLSA
                     GKFEIRPTSPGSLKMQVYANIENTSRLMGERTLRLLPVPDPTIVVGGKTAGQSIALAD
                     LQNAKVDAVLKGFLFDDVRFVVTRFEVLASIGGLTQSVTVKGNIMNDEAKSMLRKLGS
                     NQQVIISNISVRGPDGKDRPLSTAVNLKTI"
     gene            complement(16956..18155)
                     /gene="gldL"
                     /locus_tag="PLF20_01395"
     CDS             complement(16956..18155)
                     /gene="gldL"
                     /locus_tag="PLF20_01395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HOX79698.1"
                     /translation="MNLFDLVKSKKYKVFMARLYGIGASVVIIGALFKITHIRGADVM
                     LFVGLITESIIFFFSAFEPPYIDPDWSLVHPELAGLYHTEEEIEMMKQKGLIKEKKEE
                     EASLTGELDKMLKEANIQTDVIASLGQGLNNLNTTLKELNNISEVAVKSTNLVNTIDH
                     VNQNAQALSQSYKKTAEVLDKESELTQEHVTNLGGMTQNSGLASKAYGEIANKLNEES
                     NIRSALNQNIHSASQSAANLVEKYNETAQTLKNAADAIDVGENASVFKEKLSEINTKL
                     TSLNAAYELQMKAAHENASVTSTSIQTINELISKLMGSTEATGQFTQNLSALSKALES
                     QINGSNLQTQKTEEVSQAYKELLDRINAAIAHTENYQKEAKNLAEKMADLTRIYGNML
                     SAMTIKV"
     gene            complement(18202..19617)
                     /locus_tag="PLF20_01400"
     CDS             complement(18202..19617)
                     /locus_tag="PLF20_01400"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOX79699.1"
                     /translation="MKKLLYLVVAIIALASCRNTGSGELTGVPDRPKFYQPDPYGMTF
                     IPLGSFTMGTGDQDVPYANVHHPKTISVAAFYMDETEITNNEYRQFVYWVRDSLAHTI
                     LGNAGSDYGEHFVSDKQGQPIQYGTTQTYYKINWKQKIDWEGKRLKQGEPNPLDEMYL
                     PEEERFFRRKEIDTRKLFYRYYWVDLPKSARPENRYIFNRDQQGKITDEGGTYPQNIV
                     AEGNRRAFVKEEVVPVYPDTLVWISDFTYAYNEPMAKTYFWHPAYDHYPVVGVNWKQA
                     EAFCVWRTEYMNSYLSTRKREALVNDFRLPTESEWEWAARGGNELNPYPWGGPYLRNQ
                     KGCFLANFKPNRGNYVADGGLQTVIVAHYPPNDFGLYDMAGNVAEWTNSAFDESSYNF
                     TWDLNSSYTYHANYDDPAVMKRKVVRGGSWKDIGYFLQVSTRTFEYQDSAKSYIGFRC
                     VQSYLGRNKDDNPSKSSEIYN"
     gene            complement(19694..20650)
                     /locus_tag="PLF20_01405"
     CDS             complement(19694..20650)
                     /locus_tag="PLF20_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOX79700.1"
                     /translation="MKSTTWVNIALLLVFSGGKTLAQQEPQFTHNVFTRMAINPAYAG
                     SSGDISVTGLMRQQWVGFKDMDGEKVAPQTYLLTADMPVSLVHGGLGISITSDQLGFE
                     NNVGVRLNYAYRTVAGNGDLAFGPVIGFLNKSIDFGKLKPTQSGDPVLGSAEESTMMM
                     DVGLGAYYEVKNKYFLGISTSQLIQTSSSIGVDATNYNLKRHYYAHGGYIYTLPNNPL
                     IKLQPSLFIKTDFVSTQFDVNLSALYNERFCGGISYRLQDAIAFLLGLSYKSLIFGYS
                     YDLTTSRLGSAGSSGSHEIFLNYRFKLQLGKNPRSYRNTRYL"
     gene            21063..21968
                     /locus_tag="PLF20_01410"
     CDS             21063..21968
                     /locus_tag="PLF20_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOX79701.1"
                     /translation="MQKRRAIAKEIIVSYGLIVIGAFIMAAGFVLFINPYKIVPGGVY
                     GIGIVVHYITKGLIPFWPEGIPIGLVGLSLDIPLILLGMHLLGTKFGTKTILGSFLMS
                     SFMMVLTYFIGEHDPLGLQEDILLSCIFGGLTIGLGLGLIFRSRATSGGSDIIAMILS
                     KYSKLPVGQLLILVDSTIVLIGLATFKDWRIPLYSWIVIYITGKVIDLVVEGVNFEKS
                     VFIISDHPEAIRQKIIYDLRRGGTFIEGEGMYNGKPRRIIHTVMSRREVSLLLDHVRR
                     IDPDAFITVTDASEILGEGFKSLNE"
     gene            21993..22886
                     /locus_tag="PLF20_01415"
     CDS             21993..22886
                     /locus_tag="PLF20_01415"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOX79702.1"
                     /translation="MAFISVETFPSKRWFAAYGKILLGSLLVAFGYVLFITPYKIVPG
                     GVYGISIIIHYLLGTPVGLTALAFNIPITLLATKVLGPRYGAKTVTGFILTSLFIDGL
                     TYFLGNDPLKISDEMLMSSLYGGLLIGTGVGLIFRARASCGGTDVIAMMISKFIHQPV
                     GQIMIYVDSLIVLGGFIAFKDWKIPLYSLVTIFMVGRMVDVVMEGLNYNKCLLIVSDK
                     QKEISQRIIHDLKRGGTIFQSKGIYTGKEHFLIFTVMSRSEMIKLKSFIRQIDPKAFI
                     TVMDTSEILGKGFRPLNENID"
     gene            23192..23866
                     /locus_tag="PLF20_01420"
     CDS             23192..23866
                     /locus_tag="PLF20_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOX79703.1"
                     /translation="MKTIRKILCFSVLLLIGSSIYAQDNEPGGFRKFSIGFDFFTDFW
                     QNPPSVLNAKWYNRGSNVNILINNKMGESRFDFSYGVSFGSHNLYTDAWIKDVNQKSV
                     FEKIPDTLDHKKYKITLAYCDFPLEFKYRAKNKWRYYAGIKLGFLINHHSKYIGLDPE
                     TYAYNVKIKRDDVRYVNFWRYVVYARVGYKWFNVFGQYSLNHTFKDGQGPDMYPISVG
                     ISLMPF"
     gene            complement(23853..24518)
                     /locus_tag="PLF20_01425"
     CDS             complement(23853..24518)
                     /locus_tag="PLF20_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79704.1"
                     /translation="MKDYWKGIDTVIAQSLSVALHPLIVPLYSILLLYFNESATQFRL
                     HEFSAFQVFTPWIIFTMILPGIFVLFLRQKGKISSIYLKKRMDREGPLLFAAASAFIL
                     NVLYFFIAPNPLLLTLPLILIVSILIDWAVNKFWKISIHMTSLGGLSSIIIFFSWHRH
                     DIFPRIVLILLLIGILLGWARWRLKAHTFMQVILGWINGFVIALACLKYLPDVFSSLF
                     KMA"
     gene            complement(24523..26025)
                     /gene="rpoN"
                     /locus_tag="PLF20_01430"
     CDS             complement(24523..26025)
                     /gene="rpoN"
                     /locus_tag="PLF20_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOX79705.1"
                     /translation="MLHQKLQQKLLQKLSPQQILLMKLLQVPTLALEQRIKQELEENP
                     ALEEVDNEEVYDQEENNDFDADNLNADEVSESAEAESDEPIVDQGDEFDMEDYMSEDD
                     LDSYRYQVNNTSPDDEQHEMPLVSSTTFQENLLQQLSLKNISERTYQIASHLIGNLDD
                     SGYLNRDLESLVDDLAFLQNIQTNVRELENTLEIIHELDPPGIGARSLKECLLIQLRR
                     KPQSESVKNATTLLERYFDEFSKRHYDKILKKTHLTELELKQAIDEILKLNPKPGNIF
                     GDSFTAQQYVVPDFIIYNNDGDLELVLNSKNMPELRVNKAYYKMLESFSDTKSKKTQQ
                     DREALLFVKQKIDSARWFIDALKQRQNTLYITMKAIMDHQKEYFLTGDETKLKPMILK
                     DIAEKVGLDISTVSRVASSKYVQTPFGTFLLKSFFSESMQNAEGEEVSTREIKKILKD
                     LIESEEPQKPLTDEELMDRLKEKGYIIARRTVAKYREQLGIPVARLRKKL"
     gene            complement(26201..27589)
                     /gene="asnS"
                     /locus_tag="PLF20_01435"
     CDS             complement(26201..27589)
                     /gene="asnS"
                     /locus_tag="PLF20_01435"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOX79706.1"
                     /translation="MQRTKIIDLLRSEEIGLKVRVMGWVRTRRGNKNIAFIALNDGST
                     IHHLQIVAELEKFDEETIKRITTGCALQVEGRLVESPAKGQPVELAAEKIEILGEANP
                     ESYPLQKKGHSLEFLRSIAHLRPRTNTFGAVLRIRHALAFAIHKFFNDKGFVYVNTPI
                     ITATDAEGAGEMFRVTTLDINHPPRKPDGGVDFSKDFFGRETNLTVSGQLEAELVALA
                     LSQVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYDINENMDLAEEFLKYLISYTLEH
                     CMDDLKFLNRMCDEDLINRLENVVQSKFERLTYTEAMTILESSGLKFEFPLGWGNDLQ
                     AEHERYLVEKHFGRPVILTDYPKNIKAFYMKQNHDGKTVRAMDVLFPRIGEIIGGSQR
                     EENLEALLDRIHELGMNEKELWWYIDSRRFGTVPHSGFGLGFERLVQFVTGMSNIRDV
                     IPFPRTPQNAEF"
     gene            28066..28368
                     /locus_tag="PLF20_01440"
     CDS             28066..28368
                     /locus_tag="PLF20_01440"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOX79707.1"
                     /translation="MSKQKKLDIARLELAASRLRALAHPMRIAIIDMLTSTPKMNVTE
                     IYEKLGIEQAAASHHLNILKNKGILVSKRDGKKIYYSLKNDALTQIVDCLDRCDSH"
     gene            28753..32223
                     /gene="dnaE"
                     /locus_tag="PLF20_01445"
     CDS             28753..32223
                     /gene="dnaE"
                     /locus_tag="PLF20_01445"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOX79708.1"
                     /translation="MSEFVHLHVHSHYSLLDGAAKISELVKKAKDLGMPAIALTDHGN
                     LYGAFEMHEAASNEGIKPILGAEFYIAKKSRKDHDNRGYHLILLAKNLTGYHNICILS
                     TLAFKEGIYYVPRIDKELLQEYHEGIIASSACLGGEIAKTILQNGIDEAEKVVWEYKE
                     IFGEDFYLELMRHGIPEQETVNSALGILSKRCDVPLIATNDVHFINKSDFEAHKILIK
                     LNTDPKNLETDTNLVYTGNEYLRSPDEMAEIFKDFPEAIENTVSLAEKVEKYKLKRSI
                     LLPRFPLPEEFTDENEYLRYLTFEGSQRKYPEMTDEIKQRLDYELEVIKNMGFSGYFL
                     IVQDFINAARRMDVIVGPGRGSATGSAVAYAIGITNIDPIRYNLLFERFLNPERISMP
                     DIDVDFDDEGRQNVLNYVAEKYGEDHVAQIITFGSMAAKSAIRDVARVIGLPLSEADR
                     LAKLIPDRLGIKLQEAIEEVPELEQAAKGSDEKIQKVIEYARILEGSVRQTGTHACGV
                     IIGPDDLKKYIPMATQKDSQLLVTQFEGKSMESVGMLKMDFLGLKTLSIIREALHNIK
                     LNRSITIDIDNIPLDDPLTFQLYQKGDTIGTFQFESEGMRMYLRELKPEHIEDLIAMN
                     ALYRPGPMQYIPMYINRKKGREKVTYPHDMLRNILEDTQGIMIYQEQIMQVAQKMGGF
                     TLGSADILRRAMGKKDPVVMAQQKEIFIQGALNKGVTVETASEVFETMLKFAQYGFNR
                     SHSTAYSILAYQTAYLKANFPAEYMAAVLTHNLNDIKKITYYIDECQRMGINVLGPDV
                     NESQLNFFVNKKNEIRFGLTAIKNVGENAANSIIKERENGLYTSLEDFIFRLNLKAIN
                     KRTLESLIMAGALDGFQNIHRAQFFHKSREDEPAFIEKLLRHASLFQARKQSSQMSLF
                     GEFEEFALPKIELPACEPWDQLTRLHYEKEVTGFYISGHPLDLFKLEIDNLCTHSIKD
                     FKNDLEAHAGKEIIFAAMVSEAKERYGKNNKIYCSIVLEDFDETYSVTLFSEQYLKYK
                     HLLTPGSNILIRATAKQRFNGSSYELGINQIGLLSDALDNWVEKTQVFIPLPIMDDHL
                     IQQLDQSIRENPGTSTVSFTVFDPADQLSLNFENDNLLINTSGFARELKKIDGIFFKF
                     FKKKYWSDSL"
     gene            32268..32585
                     /gene="trxA"
                     /locus_tag="PLF20_01450"
     CDS             32268..32585
                     /gene="trxA"
                     /locus_tag="PLF20_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009291563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOX79709.1"
                     /translation="MAKILTDQNFEEVVLKSDKPVIVDLWAEWCGPCRNLSPIIEDLA
                     KEYAGKVVVGKLDVDNNPQTTIRLKVRNIPTILFFKNGEIVDKQVGTVPKSVLVNKIE
                     KLL"
     gene            32659..33591
                     /locus_tag="PLF20_01455"
     CDS             32659..33591
                     /locus_tag="PLF20_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOX79710.1"
                     /translation="MPVIDPLRFQPLTGLEFFAREVVEGFITGLHKSPYHGFSVEFAE
                     HRLYNTGEPIRNIDWKLYARTDKLFVKRFEEETNLRCQLVMDTSASMYYPPIQYPDVE
                     HPTKLLFSIYAAAALMFLFKQQRDAVGLSIFNNDISQTIQARSSAIHIHYLFTELEKL
                     IESQPPSRPHESSIADSLHLIAESIHKRSLVIIFSDMFDKAGEPELLFHALQHLRHNK
                     HEVILFYTIDKQLELNFDFENRPYRFVDIENGKEIKLNPYELRERYMTSMQNFLDELK
                     FRCGQYHIDLVEADIHEGFNQILLPYLIKRSRLY"
     gene            complement(33771..34394)
                     /locus_tag="PLF20_01460"
     CDS             complement(33771..34394)
                     /locus_tag="PLF20_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HOX79711.1"
                     /translation="MDEKYYICIGRQIGSGGKKISEKLAQILQLPVYDKELIQMASRK
                     SGLRSEIFEQFDEKGSRSLIGGLLGMSSPIPDEIYAQYYLTNESLFVIESDVIRELAA
                     QKSCIFLGRCADYVLKDCERCLNVFICANFEDRIKTIAETYHYQVNKAKDFIKKMDKK
                     RADYYNYFTNKHWGVADSYHLCINSSTLGIDETVNFIYLFVKKNFGI"
BASE COUNT        10342 a         6798 c         6873 g        10456 t
ORIGIN      
        1 ggatcaggct ttcatgcagc ctgttatatc attctccaga acttcctacc gatcaaggca
       61 gaggttatgt gttaaaacta ttcacgacaa aaaaatacca tctggtattt gaataaaatt
      121 tcttaagcaa cggaacgata aaagggaaaa ttatgtcctt aatcaaaaca taagtatctg
      181 ataatacaca ctttaaaaat aaataggaaa atcaatagtt attttgagac tgtcgttaca
      241 aaaaagtaaa cctcaggaag gttacacttt caaaatttat gagaaagaag agacaaaata
      301 ggataaaaaa cgatcaatat ccacaaaaat tgactcaaaa attgaaatct ttctaaccaa
      361 attttttact ttcggatttt aactatttac aaaaatcgag ctaagatagg caatgctgaa
      421 taaagtcagt ttcgttccat acaaatgaaa gtaattgctg aattttttgc atgcgaagaa
      481 tcaaggaatt taaaatcaaa ggaaatgatt tttgtgctcc ttactctcat ggattcgttt
      541 atagaagatt atttcgaaat attccatcag tgtctttcaa tggtttacga aaagaaccta
      601 tcaaatttat ctgggcatga tatttttctc ttttctatat caagcaaaaa tctctaactt
      661 tgagcttaat gagtttgctt taaaatttta aaaattatga aaacacgtac agaacacgac
      721 accatgggac ctgtagaagt tcctgccgat aaatactggg gagctcagac cgaacgttcg
      781 cgtcagaact tccgtattgg ccccgaaggc tctatgccca agcccatcat agaggctttt
      841 gccatattga aaaaggcggc tgcactagcc aattacgaac tgggtgtttt gcccgaagaa
      901 aaatgcaaat tgatagttac agtttgcgac gagattcttg atggcaagct ggatgatcag
      961 tttccgctgg taatctggca aaccggttcg ggaacgcaga gcaatatgaa tttgaacgaa
     1021 gtcattgcca atcgagcaga agttttgaga ggaggtactt tgggcacagg gaaaaccttt
     1081 atacacccca acgatgatgt aaacaagtcg caatcgtcga acgacacttt tcccacggcc
     1141 atgagcatag ctgcttacaa gactcttgtg gagattactt tgccaggcgt taagaaacta
     1201 cgcgatacct taagggttaa ggccaatgaa ttcaaacaca ttgtaaagac tggccgcaca
     1261 catttaatgg atgccacacc cttaacgctg ggtcaggaat tttcgggtta tgcagcccaa
     1321 ttagactatg ccattgagaa catagaagcg gccatgcctc atgtatctga gcttgccttg
     1381 ggaggaacgg ccgtaggtac aggattgaat gcacccaagg attatgatgt aaaggtagcc
     1441 gctaatattg ccgagctgac gggttatccc ttcaaaacgg ctcctaacaa atttgaggca
     1501 ttatcggctc atgatgcttt agtggaagct tcaggagctc ttaagactct tgccgttagc
     1561 ctcatgcaca ttgcaaacaa cattcgtctt tcggccagtg gccctcgctg tggaataggc
     1621 gaactccaac tgcccgaaaa tgaacccggt tcatctatca tgcccggaaa ggtaaatcct
     1681 acgcaaaacg aagccctgac catggtttgc tgtcaggtaa taggaaatga tatggccatt
     1741 gccgtagcag gcacacatgg tcattttcag ttgaatgtct ttaaaccagt aattattgcc
     1801 aatttcttgc agtcggctac cttacttggc caagcttgcg tatccttcaa cgaccattgt
     1861 gctatcggca tagaacctaa tctggccaat atcaaaaaaa acctcgaaaa ttctttgatg
     1921 ctggttacag ctctaaatcc ccacattgga tatgaaaatg ctgcaaaaat tgccaaaaaa
     1981 gctcataaag aaggactcac gctgaagcaa gctgctttag aactgggctt agtttccgcc
     2041 gaaaacttcg ataaatgggt cgacccctcg aaaatgacag gtgagctata attctttttt
     2101 caacttccat aagccgtcat ggattaactt tgcagcgtgg aatgtaaaga attggaaata
     2161 ataccccttc ggcaatggat tcaccttgac ggctttttgt tgattgccgg gccgtgtagt
     2221 gccgaaagcg aaaatcaatt attagaaacg gcacaagctc ttaggtcaag cggcaaggtt
     2281 cagattttca gggctggaat ctggaaaccc cgcacgcatc cggcaggttt tgaaggcaaa
     2341 ggtgaagaag cactgaaatg gttgcgaaat gtaaaacagc tcatggactt tatgacggcc
     2401 gtcgaagtag gtagccctcg acatgtggag cttgccctcg agaatcaggt agatgtttta
     2461 tggattggag caagaaccac cgtaaatcca ttcatggtaa gtgaaattgc ccaggccgta
     2521 aaaggtagtg atgtggccgt aatggttaaa aatcctgtaa atcctgatct tgactcatgg
     2581 gtaggtgcca tcgaaagaat tcatcaagca ggaataaaaa aactggcagc tattcatcgc
     2641 gggttttttt tctttcacaa gactccctgg cgcaatgcac ccatgtggga aatccctatc
     2701 gaattgaaac ggatctgccc ctgtctccct attatcgttg atcccagcca tatttgtggg
     2761 aaaacagacc ttattcggga tatttctcaa aaagccctcg acctcgaaat ggatggacta
     2821 atgatagaag tacatattca tcctgaacag gctcttaccg atggagaaca acaacttaac
     2881 cccatcgagt ttgatgattt gataaaacat ctgattattc gccaaaaaat caacaatctc
     2941 gacgacgata ccctcgagcg tctgcgcctc gaagtggacg agctcgatca agaacttatc
     3001 gaaatactcg ctcgtagaat ggccatgatc gatgaaattg gccattataa aaatcaaaat
     3061 cacctaacta tactccaaat taaacggtgg cagcaaatgc tgactgatcg gctcgaaagt
     3121 ggtagccaat tgggccttga taaagaattt atgcatcgat tatttcaatt ggtgcatgat
     3181 gagtcaatcc gcaggcaaca gaggattttc aactccaaca aatctaaaga ataacaagaa
     3241 tctcccatcc cggttttccc attttgctaa aatttcatca aaagttagta gatttgaaga
     3301 cgatttagtg tttgtaaact gctttgagaa tgagaatcta aagaaatttt tcctctcttc
     3361 aggcaaccca ccatcaaaat gagtgtctaa gaaatggttt taacaaaata taccgacaaa
     3421 gaaatcatcg aaggctgcat agccgggaaa cggtattttc aaaacctgtt gtacgaaaag
     3481 tacaaacgtt ctttgtttag tgtttgcctg agatatgcag taaacaaaca ggaagctgaa
     3541 gatatacttc aagaatcatt ttgcaagatt ttcagccgtt taggcgatta taaggcgcat
     3601 ggttcgttcg aaggctggtt gaaacgtatc gtcgtcaatt ataccctttc ctatctaaag
     3661 cagaataaaa aggatcctct tcacttcaat aatgccgaag tggtcgaaaa tatagctgac
     3721 gatgaagaag aaaatgacaa tgatcttttc aagaatatat cgccacaaca ctgtcaaacc
     3781 attctgaaat tcattcagga acttcctgta ggctatcgaa cggtgtttaa cctttatgcc
     3841 ttcgaaggct actcacatca gcagattgcc cagacattgc agatctcgga aaatacgagc
     3901 aaatctcaat tatcgaaagc ccgtcgccat cttaagaaca gaattcataa gtggatggaa
     3961 aagggcataa ctaccaataa cccaagttct tcatattgat ggaggagaaa cataaccata
     4021 tcgacacata tataaggcaa tccctcgatg gatttgaacc cgaaccttcc gaaagggtct
     4081 ggaaaggaat caagaggaaa cttgcatgga atgaatttgt acatttcaac atcacgaatt
     4141 tcacctccaa tatataccaa atttcggcga ccgtccttat cggtgcgggt attatcgtgg
     4201 gtctggttac tcaattgtta ttagtcgaca agattcgtca acaagcacaa gtaccgcttg
     4261 ctgaaaaaga tattcctacc atcatccagc cagaatctcc aaaacccgaa gaaaaactat
     4321 tcgattcatc ttctcctatt cgatctgaac tttccgaaaa accgcttcct tcttcgattt
     4381 cttttctccc atcctctcct tcttttgctc ccgaaccaac catagaaagc cttgaaaccc
     4441 caggtctcga aacatcaatt gccaaaaaaa cagcattcgc gattccttta cttccctctc
     4501 atggtataga ccgtatttca ctggctttac caccttcaca attgtaccag cctttgcaaa
     4561 agccggggga ttccattgcc tttggattcg aagaacccga aaagtatcct taccgttttg
     4621 ggctggaagc tggatggtgg tataatcaaa tgactctacc ttatccaggg aaagactttt
     4681 cgtattattg gaatgctgta ggcgtggcgt tcgaagtgga acacagaaat ttttatgcaa
     4741 aaaccggttt aaattttagc cgaatgttcg accgcttacc gttcgatatt tcctataagt
     4801 cgctcgatag cattcgttat gtctataatg tacattatta tgttcccgat ccttcccatc
     4861 ccgataatat tattctgatt acctccaggg agactttata tgattcgata actcataatc
     4921 aaaatatttt cacgacggga acatataatt atttgaacat tccctttcaa ctgggttgta
     4981 aatttctaaa aacaggaagt tttgccttcg atgcttatgc gggatgtggc ctgcaattct
     5041 tgatcagtaa aaaagaacct ttgccggtaa tcttcaatta tatgaagaca ggcctgaatt
     5101 tgaatccttc tttagttgat cactacaatt tcaattattt tttctttgga ggattgaata
     5161 ttcgatactt tattaatgaa aacctcaaac tcgaactgca gcctgtatat cagtattata
     5221 tgaaatcatt gggtgaccat gaaggagcag ggaaccccta ttcgtggggt ttaaaaatag
     5281 gcattggtta ttatttttaa aagaatttac attcttgttc attttttact aaatttgttg
     5341 actgataatt gccttccacc ttcgtgttat atgccatttt aatttatgta taattctaag
     5401 gtttacattc gtcttatcaa ggttgcagct tttttgctgc cctacatggc attaatcgtt
     5461 tctaatgcag aaaggttgca ggcacaagaa ggccccagaa gtgaatgtca ggcttccttt
     5521 agttatactt atgattcgat gaatttttta gggatttatt ttattgatca atcaacgggc
     5581 tcatttggtt catggcaatg ggattttgga gacggatggg gaagttctga acagaatcct
     5641 tttcatgctt atactcaggc agggcaatat catgtttgct tgaccatttc ggccgaaggt
     5701 aaagtttgtt atgatgtaga atgtaaggat gttagagttc caagaccatc gaattgccag
     5761 agcacattta cttgggctgc cgattcagca aatccatttt tcatccgttt tacttgccac
     5821 ccaggagaag atgccgatga ttatttttgg tcttttggag atggtgatac ttcttctctt
     5881 tgcaatccat gccacgtatt tgccgacacg ggtgtttata tagttaccct tatatcgagt
     5941 aatcgcctga atccatcctt ttgttatagt gtttacatcg acacggtaca tattaaaata
     6001 gagccttgca tttcgtcttt ctttgctcac ccttctttat tcaatcctct tgaggttcaa
     6061 tttattgcca ctccctccgg gaatattgat cattactact gggattttgg cgatggccgc
     6121 acttcaatgg agcccaaccc catttatata tacagtgata cgggaatctt cacggtttgc
     6181 ctcacggttt ggaatggcaa ctatatgtcc tattgcctcg atcagagttg tcaagaggtg
     6241 aagattcgta tttccaaatg ccaggctgat tttagctggc tgcaagatac tatttatccg
     6301 cttaaatttc gattcattaa ccaatcttat ggagttttga atgattttct ttgggatttt
     6361 ggagatggag atacaagcca tcaaatcaat ccttttcatg ccttcccttc gccaggtgat
     6421 tatcacgtga agctcagagt aagaaatttt atgcatcccg agttttgtac cgattcaaaa
     6481 gaaaaaattg tagccacagg tgatctggaa tgcattgctg atttttcatg gaagatagat
     6541 tcactcaagc ctcttggagt tatttttact tcactcatct caggttcacc cgattacatc
     6601 cactgggatt ttggcgatgg cactaccgac agtaccctcc atcccttcca ttttttccct
     6661 gatacaggca tttacagagt ctctctcgtc gtaagaaatg atttttatcc tgagtactgt
     6721 gccgatacca atgtccagca ggtccatgtt tacctcaaac accatccaaa gacagatttt
     6781 acctttgttt tcgatagcct ttctctaact ccaaatcttt tccattttta tggacaagca
     6841 accagttatc aactccaatc gtggttatgg actttcggag atggcacttc ctccaatttg
     6901 cagaatccag ttcatatttt tccacaggtt caaactttta atgtatgtct gaagattaca
     6961 gatttccttc caccaaaata ttatctgact gataaatgtt gcaaaacgat tagtacacat
     7021 gagtatttca accttggtgg ttcggtgtat gatgggaatt tgcctatcaa taatcctcaa
     7081 cctaaaggcg ataccgctct ggttacccta tacaagatct atccggggca aatgataatc
     7141 ccatggcgta gtggtacttt ccataattta ggatattatt ggtttacttc gcttctggaa
     7201 ggagagtatc tcattaaggc tcaactaact cctaagtcgg ttcaggcaaa taagtttttc
     7261 cctacatggg cgccgcaaac tctcttatgg caatcgtcca accccgtttt attgaacgaa
     7321 aacatcttca atgcaaatgt gcatcttgtc caacggccat acttcgagca aggtccaggt
     7381 tcggtctatg gtatggtaat tgtgacgtcc gatcctttac ttcccacagg gaatcctgct
     7441 tcaaacattt gcgtttttct gctcgatgac aatcatcaat tcttagattt taccctgtca
     7501 gattctctcg gacgatttca tttcgataaa ctgccgtggg gaacctatgt cttcatccct
     7561 gatgctaccg gaatgtcata taccactgac acggccattg tgaatgctca gaaacctcaa
     7621 gtagatgata tattgttgaa gatgtggaat acggatggaa tgtctgctcc tcctatcgaa
     7681 gacactcctt tgttgagtat ctggcccaat cctgcttcgg atggtataaa gttgaaaatt
     7741 cgactaacta aaccgtgtgg agtgaatatc gaacttattt ctgctactgg tcaaatattg
     7801 aattcacatt tttggcatgc ttctccttcc agtaatgaat tctatttgaa tttgcagggt
     7861 ttctctcaag gaatatatct gttaagggtg ataactccct tacaaagtca tacttacaaa
     7921 attctaaaaa gataggattc cctcccgata aatcccatga taacaaaaac gagaaaaaat
     7981 atttttttaa aaaattcttc acaattataa atttataaat tcgaagaaaa attatcctat
     8041 ggctatttta ggtatcgatg tgggaggcag tggcattaag ggagccttag tagaaaatgg
     8101 acaagttata accgagcgat tacgcattcc tacccctcag ccttctactc ccgaagccat
     8161 tgccgtaact ttgaacgaaa taatgaaagc cttcaactac aaaggagcaa taggctgcgg
     8221 gtttcccgct ttgatcctgg atggagtgat aaaaactgct gcaaatattc ataaaagctg
     8281 gattgatgtt ccagccgaaa aattgtttac caaaacgaca ggacaaccgg ttttcctaat
     8341 caatgatgca gatgccgccg gacttgcaga aataaattat ggagcaggta aagacaaaat
     8401 gggaaccata tttgtcataa ccattggtac cggcttggga agtgctattt tcatcaatgg
     8461 acaattagtg cccaatactg aacttggtca tttgaaaatg tatggtaaga gtgccgagaa
     8521 atattgcagt gatgccgtca gaaccaagaa aaacctaagc tggaaacagt gggcaatgcg
     8581 ttttaatgaa tatctggctt atatagaatt tttattctcg ccacaactca ttattctggg
     8641 tggaggagca agtaaaaaat tcgatcagtt tgctccttac ctgaaatcgc gaaccgaaat
     8701 agttcctgct gaactgaaaa atcatgcagg cattattggg gcagctgttt gggctcattc
     8761 caaatctcaa tcacaaacaa tttctctgat acaccaagac aatctgttgt aattttgcag
     8821 cggttctaaa gttgttcgtt tgcgcatacc cgaagtcgtc gatctttatc tgaatcatcc
     8881 aggtgtggag gcaatttgta gccatcttaa agattcagac ctcccccgca tccatcttag
     8941 cggagcgaaa ggatcatccc tttcccttat tacggcagca attttcaaac taacttttca
     9001 aatccattta ctcattgccg aagataaaga aagagcagct tatttattga atgatattga
     9061 aaatctcctt ggtgacagag acaaaccatt cgaaaaacgc agagtattgt tcttcccgac
     9121 ttcatggcga aaaccctacg agattgaaca aaccgacaat gccaatgtgc tcgccagagc
     9181 cgaagccctt aataaaatca tgacatgggg tcggaatctg atcatagttt cttatcccga
     9241 agctctggcc gaaaaggtgg ttacaaaaaa aagcctgaag aagaatacat tcaccatcca
     9301 tgcaggcgaa gcagtctcac tcgacttctt taccgatatg cttattgaat atggcttcga
     9361 acgtgtcgat tttgtggttg aacccggaca gttcgctacc agaggaggaa tagtagatgt
     9421 tttcacattc agccacgaat atccttgcag gattgagttc gatggggata ttacggaatc
     9481 catccgaact ttcgatcctg cttcccagct ttctattcag aaaatttccc gcatagactt
     9541 gctccctaat accgaggagt taatgctttc agaaaaaaga gaaaacctgc tcgaatatat
     9601 tcctcaaaat gccatgatat ggatcgaaaa tctcgagttt acttctgata tcatcgaaag
     9661 ggaattcaat aaggttagtg agatctatat ctctttgaaa ggaccccttg cccacagagc
     9721 gcccgaagat ttatatactg tacctgatga atttaccgct tctttactga aacatcctct
     9781 gattttcact ggcttgaatg ctttgcctgg ttcgaaaccc gtcgctttca ataccatttc
     9841 tcaatcgaac ttcaacaaaa actttgagct attgctttct gacttgcgca tctatgatca
     9901 gcaaaactat tcattgttta ttttttccga taactcccga caattagaac gtattcggca
     9961 gattatcggt gatttgagtt attcacaaaa actccctccg gttatatttc aaggtgtaaa
    10021 ctattccttg cacgagggct ttatcgatca ccaaattcgg gtagtatgct ataccgatca
    10081 tcaaattttc gatcgttacc atcgttacag acttcgtgaa ggttttgccg gtaaggaagc
    10141 tctcacactg aaagaactga ataatttgaa acctggcgat tatgtaactc atatcgatca
    10201 cggtattggt cgttttgatg gacttgaaat catcgaaaat aacgggcaca aacaggaggt
    10261 tattcgcata ttctatcaaa ataatgatat tctttatgta agtattcatt cattgcaccg
    10321 aattgcgaaa tacaccggaa gggatggcac acctcctact ctgagccgtt tgggcagcaa
    10381 tgcatggaat aatctaaagc aacgcaccaa acaaaaggta aaagatattg ctcgtgacct
    10441 tattgtgctt tatgcgaaac gcaaagccag tgcaggtttc gagttttcgc ccgatactta
    10501 tttacaaaat gaattggagg cttcatttat ctacgaagat acacctgacc aatacaaggc
    10561 aactgtggat atcaaggttg acatggaaaa atccattcct atggatcgcc taatttgtgg
    10621 tgacgttggt tttggaaaga ccgaagtagc tatacgtgct tcatttaaag ccgtggccga
    10681 tagtaaacag gttgcagttt tggtgcctac caccattctg gctcttcaac attaccgtac
    10741 gtttaacgag agattaaagg attttccatg cagggtggaa tatttgaacc gttttaaatc
    10801 gaacacggag caaaagaaat tactggcaga actcgaaaaa ggcagaatag atatcatcat
    10861 tggcactcat cgcattttga gcaaagacgt caagtttaaa gatttgggtc tggtaattat
    10921 agatgaagaa caaaaatttg gtgtagccgc taaagaaaaa ttaaaagcct tacgaataaa
    10981 catcgatact ctcacactca cagctacacc tattcctcgt acactccagt tttccctatt
    11041 gggagccaga gatttatcgg taatcaatac tcctccacct aatcgccaac ctgttcaaac
    11101 cgaaatccgt tcgtttagtg aggaaactat tcgtgatgct atttattacg aactaaggcg
    11161 aggaggacag gtattttttg ttcataatcg cattcaaaat attatggaag ttgcaggtat
    11221 gctttcacga ttcgttcccg atgcaagaat tgcagttgct catggacaga tggaaggtca
    11281 caaactggaa gagatcatgc tcgacttcat agaaggagat tatgatgtct tggtgtctac
    11341 aacgattatc gaatcggggc tggatattcc taatgtgaat acgattatta tcaatgatgc
    11401 tcagcatttt ggcttgagtg aattgcacca gttaagaggt cgtgtaggac gcagcaataa
    11461 gaaggcattt tgttatctat tggcaccgcc tttggcttca cttagcgatg acgcccgtag
    11521 acgtctcagg gctatagagg aattttccga tttaggcagt ggtttcaata ttgccatgcg
    11581 cgatctcgat atccgaggag caggtaacct tctcggagcc gaacaaagcg ggtttatagc
    11641 cgatctgggt tatgaaacct atcaaaagat cctcgacgaa gccatgcaag aacttagaaa
    11701 aactgaatac tccgatctct tcccatccga agaatcaaat acttatgtaa atgaatgcac
    11761 cttcgaaacc gatctggaaa tgcgcatccc cgtcgaatat atcggcaata tcaccgaacg
    11821 cctcgcactt tataaagaac tcgatggaat ggaaaacgaa gacgacctac aggaatttgc
    11881 acttcgtctc gaagaccgct tcggcccact gcctgctcca gccctcgacc ttctcgatag
    11941 tattcgcctg agaaaaatgg ggaaaagcct cggcatggaa aaaatcgttc tcaaaaatgg
    12001 acaaatggtt tgttatttca ttaccgaccc caattcgcca ttttataaat cgaacgactt
    12061 tacccatgtg cttcagtaca ttcaaactca ccaacgaatt tgtcaattga aagaaaataa
    12121 gaataaactc ctcctcacca ttcatcatgt agatatggtt caaaaagcca tacagattct
    12181 ctctgaattt catgaaaaag aaaaaaatga aattagagag gcattgaaat agatcatcat
    12241 aagatgcatg ttatttcgaa aaataaattg ttagcggtaa aagctaaaaa tattttattt
    12301 atggaggaaa gatttttcaa gatcagaaaa atatgatttc acaaagttag atagaaaaaa
    12361 cgccctatgt ttctcaatgt aaaaaaatat taattaatag acgtacactg agtttcataa
    12421 agagaaagtc tcgtggctct ttgtgaatga ttttaacccc taaaatccca atttatacgt
    12481 aattaaataa aggaagattg ctgtatatgt ttgtatacga ccatcttatt ataattttgc
    12541 aattgtgtat ttaggtaatt tttagcattt tacctttaaa atttcagaac tcatgatttg
    12601 gtgttaatca ggagttctga atttataaaa ccaaactcac tatttttgat aaacattcta
    12661 tacattgaat cagtgcctta atatcaagat aagggataga ttggatcata tagcatgatg
    12721 tgacattctt caaatcttga atatattaaa agtttggttt ttcttctttg gtgtttttac
    12781 aaaatgcttt aatttagcca tgttttatga ataaacattg caaaacaact taacactgca
    12841 aacagagagt aaatgaaaac aaagaaatgg attattcttc tttccctgtt attcatgggg
    12901 atcatcaggg tatattcgca agatatcgaa tatgcacggt ttatagtaaa aaccttgact
    12961 tcgtcagaat acaaaggacg agggtacata ggcaaaggcg ataaaaaagc agcggaattc
    13021 attgtaggac agttcgaaaa atataggctg caacctttcg ggaaagatta cttacagaaa
    13081 tactccttca gcatcaatac ctttcccgag aaaatggagg tggtggtaga tgatcgcaaa
    13141 ttaatgtcag ggaaggatta tgtgatatca ttggcatcta agggagtaaa agcctcttac
    13201 gaattagaat ggcttatcga cgacagcatt cctgaaagta gtcgctggaa ggaattatgt
    13261 agtatagatt taacacataa actcgttgtt actgatgaat ttcataaaaa tcttgttgag
    13321 gttaatttat tgggcgcagc tggatatgta ttcctcaaag acagcaatga aattatctca
    13381 tggaaggttt ctaatggtag aattttgaaa aattttttag tgatggatat tcagaaagag
    13441 aagttaggga aaacaactca gagaatatcc gttgaattca agaataaata tgaagagaat
    13501 tatcaaactc agaatgtgat aggaatgata agaggattag ggagtccaga tacttttcta
    13561 gtgatttcgg cccattatga tcatttaggg atgatgggcg aaaagaccat gtatccgggg
    13621 gccaatgaca atgcgagtgg tgtaggtatg atgctcgatt tggcccgaca tttcagtttg
    13681 cctggcaatg aaccatacta ttctcttgta tttattgcct tcagtggaga agaagctgga
    13741 ttgatgggct caaattatta tgcggagcat cctttattcc cattagcaca aatcaaattc
    13801 ctcatcaatc tcgacatggt aggcagtgga agcgaaggca ttggagtagt taatggccag
    13861 catttgaaga aagcaggcct gttgcttcaa caattaaacg ataagggtaa atatttatca
    13921 gaggtggctc tgaggggaga atcgaaaaac agtgaccatt tcccttttta taacaaaggg
    13981 gtttcttgtt tctttattta tactatgggg aaagagtggc cttattatca tactcctgag
    14041 gatacctgtt gcccatcttt cactgcttac ataggattgt tcgatctttt gaatgaattt
    14101 atcgttaagg ttaggtttct ttaataaagg ttgtctatgc aaatagatca tttactttat
    14161 attagatatc caaaagggca tctaccaaaa ttcaaagtct aaaaagtttc gatggtcaag
    14221 gttactctta tggttagaga tttgacataa agtttgcctt gcttcggttt aaggcgcaga
    14281 cagaaggcta tatatgaaaa aaatgaaggg gtaaagggtt tttcctgata ataaaaaagc
    14341 ccggttttaa aaccaggcta ataaaaggga aacacaaatg ggaaattaat attcccacaa
    14401 atcttgttca aactgcagta tctcactttt tattctttcc gattctaaga gagcatctaa
    14461 tccagaggca taatcggata tcattctatc gtaaacgttt tcttctttat agatatagct
    14521 tgcgaatttt cgttttgtga atatatcgtc gtaggtacgc cattctgcgt cattctttgg
    14581 attataggcc tcattgttta ccaaccagaa acgagcttca gggtaataaa tccagaaagt
    14641 aggagaaggg tcttcgaggt attgtttttg accgttgctc ataatgaaac ggtaataaac
    14701 cgggcagatt gcatgaatac gcacatcgag ctggcttcgt cttttatcga agaaccagtc
    14761 ttctataatg aagtatttta ccacatcctc ggctttcagt tcatagactt tttcctgtgt
    14821 ggtttcattt cccaatgaat ctatataggt atcgcggtat tcgaaactaa ggcgtctttt
    14881 gacgtcatcc acagttgtat agcgttcggc taaatcttcg tcggtatatg cagtcaagta
    14941 attcgagcca taaggatttt cctgttgagg agtaataacg gcttcgaaca gtacagaagc
    15001 cagtgatttt cgatcacgaa cgggacgaag gggataatat aggggtaaat tttgtttttc
    15061 ccttaaatcg atgactctcc aaatccgttt agcccatatc acgtcggctt cacgcacagg
    15121 tgcataaggc acaggccttt ttcgattggg tgtatgtatc ttgggccagg gcctttccat
    15181 aggcttcact ttaggaggct caattacttg agcttcgctt ccccaacaat ataatgaaag
    15241 acctaaaata acaacagcaa ttttgcgata cattttcaac attatatttt aaattgtttt
    15301 caagtttacg gccgtactca gtggcctatc cttaccatcg ggtcctctca ctgaaatgtt
    15361 actgatgata acctgctggt tactgcctaa ttttctcaac atgcttttag cttcatcatt
    15421 cattatattt ccttttactg ttacactttg agtaagtcca ccaatgctgg cgaggacttc
    15481 gaatcgtgtt acgacaaatc gtacatcatc gaaaaggaaa cctttgagga cggcgtctac
    15541 tttagcattt tgcaaatcag ccagtgctat ggattgtccg gcagttttcc ctcccacaac
    15601 gatggtagga tcgggtacgg ggagaagacg caatgtgcgt tcacccatta agcgagaagt
    15661 attttcgata ttcgcataaa cctgcatttt taaactacct gggctagtag gtctaatttc
    15721 gaatttaccg gctgaaaggc gtttaacttc agcaggtcca gaaattttaa tatcgaggtt
    15781 atcgggtggg attcctggta cagagactgt tataggatta tctaaaccga tataaagaac
    15841 cgacatttta tcgagggaga taacagccac aggctcttga actacaaatt catcggcaaa
    15901 ttcatattgc tcttccactc ctaaaggatt caagatggta atcactccag caaatttttt
    15961 agggccataa tccattccgg cagtgctgat ttttaagtgg gccatgccca attcaccgga
    16021 agtaacattt gaagctccac ttgcgtaaga aggcaaccat ttgtctattc cgagacggta
    16081 aattatatta ggggtagaag tagtatcata agctgcaact aatacttcgg cctggtattc
    16141 ttctccttta aaaaggtatt tccgattggg gataactttt gcagctattt tattgaattt
    16201 aaaatcagtg gctcctatat agctcgacaa aagagaaacc acatcgaatt cggcattgcg
    16261 tacctcggct atatacttgt taaggattac aaggtcggca gaaagaatgg tatggtaaaa
    16321 gtaatgtttt tcccaggttt gtactgcacc atattcatct cgaaattttc cttccgtaat
    16381 caaacctaat tgaagttttg gatggtcttc gtcttttacc agttgagtca ttttttcctt
    16441 ataatcctta aaaactcttt tcaaatcttc ggctttccct ttcgaaacat cggttccaag
    16501 aaagaatctt gtaggatcgt caaaattatc tttggccttg atacggataa gaggagtggt
    16561 atcggcctgt tctaaagtct ttatactacg gtcggagaaa acgattaatt cgtttttcat
    16621 gtgattcaga taatttacta agctatccga caattttttt gcaatcaatg ccttatcgta
    16681 ccattctttg gccgaagctc ctccaattgt tgcttgactt tcgaatttcg aatatgtttc
    16741 tcttagtttt ttatcgaagc tattgttggt ttgttccatg ctttcattca cggttacaaa
    16801 cgcctgtatg atgtctttcg aaacgtttaa ggctaacata gcagtgagta ccaaatacat
    16861 cattgctatc atcttttgcc gtggggtttc tttgtaacca gccattagaa ataggtttta
    16921 gtgaataaca attattatat agggttagga acagcttaaa ctttaatagt cattgctgaa
    16981 agcatattgc cgtaaatacg ggtaaggtca gccattttct cggctaggtt tttggcttct
    17041 ttttgataat tttctgtatg agctatagcg gcattgattc tgtcgagcaa ttccttgtat
    17101 gcttgtgaaa cctcctcagt cttctgggtt tgcagattag aaccattgat ctgagattca
    17161 agggctttgc tcaaggcaga aagattttgt gtaaattggc ccgtggcttc ggttgaaccc
    17221 atcaacttgc taataagttc gtttatagtt tgaatgctcg tagaagtcac actggcgttt
    17281 tcatgggcag ctttcatttg aagttcatag gctgcattaa ggcttgtcaa ttttgtattg
    17341 atttctgaaa gcttttcttt gaaaaccgag gcattttcac ctacatcaat agcatcggca
    17401 gcatttttaa gagtttgagc cgtttcgtta tacttttcaa caagattagc agccgattgt
    17461 gaagctgagt gaatattctg atttagtgcc gatctaatgt tgctttcttc attcaattta
    17521 ttggcaattt caccataagc cttactggct aaaccagaat tttgagtcat tcctcctaga
    17581 ttagtcacat gttcttgagt caattccgat tctttatcaa gaacttcggc tgtcttttta
    17641 tagctttggc tcaatgcctg agcattttgg ttcacatgat ctattgtatt taccagattt
    17701 gtggatttga ctgctacttc ggaaatgttg ttcaattctt tcagtgttgt attcaaatta
    17761 ttcaagcctt ggccgaggct tgctattaca tctgtttgaa tattcgcctc ctttagcatt
    17821 ttatcgagtt cgcccgtaag gcttgcctct tcttcttttt tttccttaat gagtcctttt
    17881 tgtttcatca tttcaatttc ttcttcagta tgataaagcc ctgcgagttc tggatgcacg
    17941 aggctccaat cggggtcgat ataaggaggt tcgaaggctg agaagaagaa aataattgat
    18001 tcggtgatta aaccaacaaa aagcatcaca tcggccccac gaatatgggt aattttgaag
    18061 agtgctccaa taatgacaac ggaagctcct attccataga gccttgccat gaaaactttg
    18121 tacttcttgc tttttacgag atcgaacaga ttcatgattt ttattgtttt gttttatggg
    18181 gttaatttgg attttctcta attagttata aatttcggat gatttggagg ggttgtcatc
    18241 tttattgcga cccagataag attggacaca gcggaagcca atataagact ttgcagagtc
    18301 ctggtattcg aaagtccgtg tagatacctg aaggaagtaa cctatgtctt tccatgaacc
    18361 gcctcttact actttgcgtt tcatcacggc aggatcatcg tagttggcat gataggtata
    18421 agatgagtta agatcccagg taaaattata ggaagattcg tcgaatgcgc tatttgtcca
    18481 ttcggctacg tttccggcca tatcgtatag gccaaagtcg ttaggtgggt aatgagccac
    18541 gataacagtt tgtaagccac cgtcggccac ataatttcct ctattaggct taaagttggc
    18601 taaaaaacag cctttttggt tgcgtaaata tgggcctccc catggataag ggttgagttc
    18661 gttgcctcca cgggcagccc attcccattc gctttcggta ggcaatctga aatcgtttac
    18721 caatgcttcc cttttacggg tcgaaagata gctattcata tattcggttc tccatacgca
    18781 gaatgcctcg gcttgtttcc agttaacacc aactacgggg taatgatcgt aagcgggatg
    18841 ccaaaaataa gtcttggcca tgggttcatt ataggcataa gtaaaatcgc ttatccagac
    18901 caaagtatcc ggataaacgg ggacaacttc ttcttttaca aaggctcttc tgtttccttc
    18961 agccactata ttctgcggat aggttcctcc ttcatcagta attttccctt gctggtcgcg
    19021 attgaaaata tagcgatttt cgggcctggc agatttgggg agatcgaccc aataataacg
    19081 gtaaaagagc tttcgggtat caatttcctt acgacgaaag aagcgttctt cttcgggcaa
    19141 gtacatctca tcgagcggat tgggctctcc ttgtttgagg cgtttccctt cccagtcgat
    19201 cttctgtttc caattaattt tgtaataggt ttgagtggta ccatattgaa tgggttgtcc
    19261 ctgtttgtcg gaaacaaagt gttcgccata atccgaaccg gcattcccta agatagtatg
    19321 agctaatgaa tctcgtaccc aataaacaaa ctgccggtat tcattgttgg taatttcggt
    19381 ttcgtccata tagaatgcgg ctaccgagat ggtttttgga tgatgaacat ttgcataggg
    19441 cacatcctga tcgcccgtac ccatggtaaa actgcctaaa ggaatgaatg tcataccgta
    19501 agggtcgggt tgataaaact tcggcctgtc gggaacaccg gtcaattccc cgctgcctgt
    19561 attgcggcag ctagccaaag caatgattgc aacaactaaa tacagcagtt ttttcatttt
    19621 tatcaacggt tataatcgat taaaacttaa tttttcaaat caaaaattct gatggcacaa
    19681 aaataaaaaa aactcataag tatcttgtat ttctataact gcgaggattt ttaccgagct
    19741 gcagtttgaa acggtaattc agaaaaattt catgactgcc agaacttccc gcacttccaa
    19801 gacgagaagt agttaaatcg taggagtagc caaaaataag acttttgtaa ctcaacccta
    19861 ataaaaatgc aatagcatcc tgcaatcggt agctgattcc accacagaat ctctcattgt
    19921 aaagggcaga tagatttacg tcaaactgtg tagaaacgaa gtcggttttt atgaagagac
    19981 tgggttgaag tttgattaaa ggattattgg gtaaagtata aatatatccc ccatgagcat
    20041 agtaatggcg tttcaggttg tagttcgttg catcgacgcc gatgctcgag cttgtttgaa
    20101 taagctgtga agtagaaatg cctaagaagt atttgttttt cacttcgtaa taagccccca
    20161 ggccaacatc catcatcatt gtactttcct cagcactgcc caacacagga tcgccactct
    20221 gggtaggttt gagtttgccg aaatctatag atttatttaa aaaaccaatc accggcccaa
    20281 atgccaggtc tccatttccg gcaacagttc tatatgcata attcaaacgc acacctacat
    20341 tattttcaaa gccaagctga tcggaagtga tagagattcc aagacctccg tgaaccaaac
    20401 ttacgggcat atctgccgtg agaagataag tttggggtgc caccttttca ccatccatat
    20461 ctttgaaccc aacccattgc tgacgcatca aacctgttac agaaatatcg ccagaacttc
    20521 ctgcataagc aggattaatg gccatccgcg taaacacatt atgggtaaac tgaggctctt
    20581 gctgggctaa tgttttaccc ccactaaaaa ccagtaagag tgcaatattc acccatgttg
    20641 tgcttttcat tagattcatt tccgcatccc cactgaaatc gggcataaaa taacacataa
    20701 aatttcgact atacaataaa tcttctaaaa tttcatccct ttctccgata acaactttcc
    20761 cgatcgattc ttcattatca agttattata catttcattc cgttcgatca aaaaggtaaa
    20821 atcgttcaac tgaggtttga aaacttcggt acaccacaat taactacaaa tatagtctat
    20881 ttattgtaaa ccaaactaat ttttagcccc ttgaaggaaa aatttttgat atttttatcg
    20941 aagggctcat acaaacattg aacaaatacg tactatttcg gagttcgaaa gatatgttct
    21001 ctttagttta ggttttgtaa ttttgcaaaa tattcaatat ttcaaagatt tagatacttt
    21061 ttatgcaaaa aagaagggca atcgccaagg aaatcattgt tagttatggc cttattgtga
    21121 tcggagcctt tatcatggcg gccggtttcg ttctatttat caatccttac aagatagttc
    21181 cgggaggagt atatgggatt ggtattgtgg tgcattatat taccaaagga ttgattccat
    21241 tctggcccga agggattccc attgggctag ttggattaag tctagatata cctcttattc
    21301 ttttaggaat gcatttgctc ggaacaaaat ttggtacgaa aaccattctg ggctcttttt
    21361 tgatgtcgtc atttatgatg gttttgactt attttatagg agaacacgac cctttaggct
    21421 tgcaggaaga tattctgttg agctgtattt ttggaggact cactatagga ttaggcttag
    21481 gattgatttt caggtcgaga gcgacttcag gaggatcgga tattattgcc atgattcttt
    21541 caaaatattc aaaacttcct gtaggacaat tgcttatatt ggttgactct accattgtcc
    21601 tgataggttt ggcaactttt aaggattggc gtattccatt atattcttgg atagtaattt
    21661 atatcactgg taaagtgatt gacctggttg tggaaggggt aaatttcgag aaatcggtat
    21721 tcattatttc cgatcacccc gaagccatcc ggcaaaagat tatttatgac ctgagaagag
    21781 ggggtacttt tattgaaggc gagggaatgt acaatggcaa accccgtaga attatccata
    21841 cagttatgtc gagacgtgag gtttcgcttt tactcgatca tgttcggaga atcgaccctg
    21901 atgcctttat taccgtaact gatgccagtg agatcctggg tgaaggattc aaatctttaa
    21961 acgaatagaa caattataat tcccaaattt ctatggcctt tatatctgtc gaaacatttc
    22021 ccagcaaacg atggttcgcg gcttatggca agatcctttt agggtcgttg cttgtggcgt
    22081 ttggatatgt tttgttcatc actccttata aaatcgtgcc cgggggtgtc tatggaataa
    22141 gtattatcat tcactattta ttgggcactc ctgtgggttt aactgctctg gcttttaata
    22201 tccccattac tctccttgcc acgaaagttt taggccccag atatggagca aaaacagtta
    22261 ccggattcat tctgacttct ctgtttattg atggattaac ttattttctt ggtaatgatc
    22321 ctctgaaaat tagcgacgaa atgcttatgt cgtcgttata tggcggattg ttgataggaa
    22381 ctggggttgg tttgatattc agagccagag cttcgtgtgg aggcactgac gtgattgcta
    22441 tgatgatttc gaaattcatt catcagcccg tggggcaaat tatgatctat gtggattctt
    22501 tgattgtact gggaggtttt attgctttca aagattggaa gattccactg tattctctgg
    22561 taactatttt catggtgggc aggatggtgg atgtcgtgat ggaaggattg aattacaaca
    22621 aatgtttgct gattgtatcg gacaagcaaa aagaaatttc gcaaagaatc attcatgatt
    22681 taaaaagggg cggaactatt ttccaatcga agggaatcta tacgggtaag gaacactttt
    22741 taatattcac agtgatgagc cgaagtgaaa tgatcaaatt aaagtcgttt attcgtcaaa
    22801 tcgaccctaa ggcttttatt accgtgatgg atacaagcga aattttaggc aagggattcc
    22861 gtcctcttaa cgagaatata gattaatgat actttttcag gcctttaggc ttttgttgat
    22921 ttaaaaaatg attcgatgct aggatgattc tccgaatata taagattaag atcaagacat
    22981 agcatggcaa tccgactcga ttccaatgga aactatgttc tttgcatgtg ttgacttcat
    23041 tctgaaattt ttcgacttgg cagttcttaa aataatcatg gaggaatgcc gtttcttgat
    23101 ttattctata tagattactc attctatcga tgtgtttccg aatagtgaat gtcaataatt
    23161 ttatagtttt gccatataat atcatttatt tatgaaaact atccgtaaga tactgtgttt
    23221 ttcggtttta ttattgatag gttcaagcat ttatgctcag gataatgaac cgggaggctt
    23281 tcgaaaattt tcgattggtt ttgatttctt taccgatttt tggcaaaatc cgccttcagt
    23341 gcttaatgca aaatggtaca accgtggttc caatgttaac atcctcatca ataacaagat
    23401 gggtgaaagc cgctttgatt tttcttatgg agtgagcttt ggatcgcata atttatatac
    23461 ggatgcatgg attaaagatg tgaaccaaaa gagtgtattt gaaaagattc ccgatactct
    23521 cgatcataaa aaatacaaga ttacacttgc ttattgcgac ttccctctcg agtttaaata
    23581 cagggctaaa aataaatggc gttattatgc cggcatcaag ctgggttttt taatcaacca
    23641 tcattcgaaa tacattggcc tcgatcccga aacgtatgcc tacaatgtga aaattaaaag
    23701 ggatgatgta cgttatgtaa atttctggag atatgttgtt tatgcccgtg tcgggtataa
    23761 atggttcaat gtatttggac aatattcctt gaatcatacc ttcaaagatg ggcaaggccc
    23821 tgatatgtat ccaatatcgg tgggaataag ccttatgcca ttttaaaaag cgaagaaaaa
    23881 acatcaggta agtactttag acaggctaaa gcaataacaa aaccatttat ccatcccaga
    23941 attacttgca tgaaagtatg tgccttgagc cgccagcgag cccatcctaa aaggatgcct
    24001 ataagcaata aaattagaac tatccgggga aaaatatcat gtcgatgcca cgaaaaaaat
    24061 attattattg aggataatcc acccaaactg gtcatgtgaa tactgatttt ccaaaattta
    24121 ttgactgccc aatctataag gatactgacg attaaaatca gaggtaatgt cagaagaaga
    24181 ggatttggag ctataaaaaa gtacaagaca ttgaggatga aagcagaagc agcggcgaat
    24241 aagagaggcc cctcacggtc cattctcttc ttgagataaa ttgacgatat ctttcctttt
    24301 tgtcgaagaa ataaaacaaa gataccgggc aatatcatgg taaaaatgat ccatggagtg
    24361 aaaacctgaa aagctgaaaa ttcatggagt cgaaattgtg tagccgattc attaaaatag
    24421 agcaaaagaa tgctatagag gggaactata agaggatgaa gtgctacaga gagggattgg
    24481 gcaataactg tgtcaatgcc tttccagtag tctttcatga atttatagtt tttttctcag
    24541 ccttgccacc gggattccca attgttcacg atatttggct actgtgcgcc gggcaatgat
    24601 atatcccttt tctttcaagc gatccatcaa ttcctcgtcg gtaagaggct tctgtggctc
    24661 ttccgattcg ataagatctt tgaggatttt tttgatttca cgtgtcgaaa cttcttcgcc
    24721 ttcagcattt tgcattgatt ctgagaaaaa tgatttaagg agaaaggttc cgaaaggagt
    24781 ttgcacatat ttactactgg ctactcgcga aacagtcgag atatccaggc ctaccttttc
    24841 ggcaatatct ttcagaatca tgggttttaa tttcgtttca tcgcctgtaa ggaaatattc
    24901 tttttggtga tccataatgg ccttcatggt aatatagaga gtattttgcc gttgtttcaa
    24961 ggcatcgata aaccagcgtg ccgaatctat cttttgtttc acgaataaca gagcttcgcg
    25021 gtcttgctgt gttttttttg attttgtatc ggaaaacgac tcgagcatct tataatatgc
    25081 tttattaacc cttaattcgg gcatattctt cgaattgagt acaagttcaa ggtctccatc
    25141 attgttataa atgataaaat caggaactac atattgttgg gcagtgaaac tatcgccaaa
    25201 gatgtttcca ggtttggggt tcagtttcaa gatttcatcg atggcctgtt tcagctcaag
    25261 ttcagtcagg tgagtttttt tcaaaatctt atcatagtgt cttttgctga attcatcgaa
    25321 atatcgttcg agaagagtag ttgcattttt cactgattct gactgaggtt ttcggcgaag
    25381 ttgaattaag aggcattctt taagcgaacg tgcgcctatg ccaggaggat cgagttcgtg
    25441 aatgatttcc agagtgtttt cgagttctct tacattggtt tgaatatttt gcaggaaagc
    25501 cagatcgtca accagagatt cgagatcgcg attcaaatag cccgaatcgt cgagattgcc
    25561 gattagatgg ctggcaattt gataggtgcg ttcagaaata tttttgaggc taagttgctg
    25621 aagcagattt tcttgaaacg tagtggaaga tactagaggc atttcatgct gctcatcatc
    25681 ggggctggtg ttatttacct gataacggta tgagtcgagg tcatcctcgc tcatgtaatc
    25741 ttccatatcg aattcatcac cctggtcaac aatgggttca tcgctttcgg cttctgcgct
    25801 ttcagaaact tcgtcggcat tcaggttatc cgcatcgaaa tcgttgtttt cttcttgatc
    25861 ataaacctct tcattgtcaa cttcttccaa tgccggattt tcttcaagtt cctgcttaat
    25921 tctttgctcg agcgccaaag taggaacctg cagcaatttc atcagtaaaa tttgctgagg
    25981 cgaaagcttc tgtaagagtt tctgttgtaa tttctgatga agcataattg attatggcgc
    26041 ttatttatac ttttcgaatt gaaatttgtt tatatcattt cgagtgggtc gattcacttg
    26101 atttgaagca tcaacaaaat taatcatttt ttatttatgg gattgccata caggaaataa
    26161 attagaaaac cccagaaata aaagggattg cctctatttg ttagaattcg gcattttggg
    26221 gtgtacgggg gaatggaatc acatcacgta tgttgctcat tcctgttacg aattgcacaa
    26281 ggcgttcgaa accaagtcca aatccactgt ggggtacggt tccaaatcgt cgcgaatcga
    26341 tgtaccacca gagttctttc tcattcattc cgagttcatg aattctatcg agcagagcct
    26401 caagattttc ttcacgctgt gaacctccaa taatttcgcc aatacgtggg aacagaacat
    26461 ccatagcacg cacggttttg ccatcgtggt tttgtttcat gtaaaaggct ttgatatttt
    26521 tcggataatc tgtaaggata accgggcggc cgaaatgttt ctcgacgagg taacgttcgt
    26581 gttctgcctg taaatcattg ccccagccta atggaaattc gaatttcaga cccgaactct
    26641 cgagaatcgt cattgcctcg gtataagtta aacgctcgaa ttttgactga accacatttt
    26701 ctaaacggtt aatcaaatct tcatcacaca tccgattcag aaatttcaag tcatccatgc
    26761 aatgttcgag ggtataggaa atcaagtatt ttagaaattc ttcggcgagg tccatatttt
    26821 cattgatatc gtaaaaggcc atttcgggtt cgatcatcca gaattctgcc aaatgtcgtg
    26881 gagtgttgga attttcggct ctgaaggtcg gtccgaaggt ataaacctgt gacagggcga
    26941 gggctacaag ttctgcttcc aattgccccg aaactgtcag attggtttcc ctgccgaaaa
    27001 aatctttcga aaaatcaaca cctccatccg gttttcttgg tggatgattg atatcgaggg
    27061 tagtcactct gaacatttca cctgcgcctt cggcatcggt agcagtaatg atgggagtat
    27121 ttacataaac gaatcctttg tcgttgaaaa atttatggat ggcaaatgcg agggcatgac
    27181 gaatacgaag aactgctcca aaagtattgg tgcgggggcg taaatgagct atggaacgta
    27241 aaaattccaa actatgacct tttttttgaa ggggataaga ttcgggatta gcttctccta
    27301 agatctcgat tttttcggcc gccaattcta cgggttggcc tttagccggc gattctacaa
    27361 gcctgccttc cacttgtaaa gcacaacccg tagtaatacg ctttatcgtt tcttcgtcaa
    27421 atttttccaa ttccgctact atttgaagat gatgaatagt agaaccatca ttcaaagcaa
    27481 taaaggcaat attcttattt ccgcgacgag tcctcaccca acccatcact cttactttca
    27541 atccaatctc ttccgatctc aatagatcaa taattttcgt tctctgcata taaagggaat
    27601 attttacatc tttttatcga gtttgcaaaa ttaagagcaa tttttgtaac gacagaaaag
    27661 tgatcgatcc tttctcccat aatagaaata atatcattgc ctcaaacgca aagctcaatg
    27721 atcttgcaaa agaatcgatc tgattgttga attttagggc agagtgcctc caagtaagta
    27781 atgatgagca aggatttctt cctcctacat gtaaaaggaa tcatgtcatt taccgacggt
    27841 ttcgaagccg tttcaaaggt ttcccttcag cttccccgac tttatcaacg cccaatttta
    27901 taacaaacca ataaacgact gataatttct taataagaaa acccaataaa tctcttggat
    27961 ttataatgct ggcatttccc ggaagaataa atcgcttatt tatatatcta cttgtttgaa
    28021 atatgggaat gattattttt gcaaaaactt aaagccttaa aaaagatgtc gaaacagaaa
    28081 aaattagata ttgctcggct ggaattggcg gcaagcagat tgcgtgcatt ggctcatcct
    28141 atgcgtatcg ccatcatcga tatgttgacg agtactccta aaatgaacgt taccgagatt
    28201 tatgaaaaat tagggataga acaagccgct gcttctcatc atttgaacat cctgaaaaac
    28261 aaaggaatat tggtttcgaa aagagatggg aaaaaaattt actactcttt gaagaatgat
    28321 gctctaaccc aaattgtcga ctgcctagac cgttgcgaca gtcattaagt caactccgaa
    28381 aagatcgtac ttcctttaga gggttgtagt ctttttgtgt ttcgaaaatg gaaataaaaa
    28441 aagaatctga aaaaataggc ttaagggaaa gaattagggg tatattttgt tgaaatataa
    28501 ttttttcaat aacgaatagt gagcttccct gtaaaggaag gagattatcc cccatcttaa
    28561 ttatactcgc cgtattttta cgtctgagaa gttcttgcct tatcgtacga aggggcattg
    28621 ttccctcaaa ttctaaagaa aagttcgagg atttatggct attttttgat attctgtcat
    28681 tcataatgcc ggggattggc taattttgga aaccacaatt caattcagaa taaataaaat
    28741 attgtcttta taatgagtga gtttgtccac ttgcatgtgc attcccatta ttcgctatta
    28801 gatggggctg caaagatttc tgagcttgta aagaaggcta aagatttagg catgcctgcc
    28861 attgcattga ccgatcatgg gaatttatat ggtgccttcg agatgcatga agcagcctca
    28921 aatgaaggaa taaaacctat tcttggagct gaattctata tcgctaaaaa aagccgtaaa
    28981 gaccatgata atagaggtta ccacttgatt ttacttgcga aaaatttaac aggataccat
    29041 aatatttgca ttttgagtac actggcattc aaagagggca tatattacgt tccaagaatc
    29101 gacaaggaat tgctccagga atatcatgag gggatcatag cttcttcggc atgtctggga
    29161 ggagagattg caaaaaccat attacagaat ggaattgatg aagccgaaaa agttgtttgg
    29221 gaatacaaag aaatttttgg tgaagatttt tatctagaat tgatgcgtca tgggattccc
    29281 gagcaggaaa cggtcaattc ggctcttggc atactttcga aaagatgcga tgttccgttg
    29341 attgctacca acgatgttca tttcatcaat aagagcgatt tcgaagcgca taaaattctc
    29401 atcaagctaa atactgatcc gaagaatctc gaaaccgaca caaatctggt atatacgggg
    29461 aatgaatatt taaggagccc ggatgagatg gcagagattt tcaaggattt tcccgaagcc
    29521 atcgaaaata cggttagctt agccgaaaaa gtggaaaaat acaagttgaa acgaagtatc
    29581 ttgcttcctc gttttccact tcctgaagaa tttactgatg aaaatgaata tttacgttat
    29641 ttgacttttg aaggtagcca aagaaaatac cctgaaatga ctgacgaaat caagcagagg
    29701 ctcgattacg agctggaagt gattaaaaac atgggatttt cgggatattt tcttatcgtg
    29761 caggatttta tcaatgctgc acggcggatg gatgtgattg taggaccagg cagaggctca
    29821 gctacaggct cggctgtagc ttatgccatt ggaattacca acatcgatcc cattcgttac
    29881 aaccttctgt tcgaacgctt tctcaacccc gaacgaatat ctatgcccga tatcgatgta
    29941 gatttcgatg atgaaggccg gcaaaacgtt ttaaattacg tagcagagaa atacggagaa
    30001 gaccatgtag cacaaattat tacttttgga agtatggcag caaaatcggc tatcagagac
    30061 gttgcaaggg taatagggct gcctctttct gaagctgacc gactggcaaa actgataccc
    30121 gacaggttgg gcattaaatt gcaggaagcc atagaggaag ttcctgaact cgaacaggcg
    30181 gcgaaaggat ctgatgaaaa gattcaaaag gtaattgaat acgccagaat cttagaaggc
    30241 tcggtaaggc aaactggaac ccacgcctgc ggtgtaatca taggccccga tgatttgaag
    30301 aaatacatac ccatggctac ccaaaaggac agccaattac tggttaccca gttcgaaggt
    30361 aaatcaatgg aatcagtggg aatgttgaaa atggatttct taggactgaa gaccttgagc
    30421 attattcgag aagcattaca caacatcaag ctcaatcgct ctattactat tgacatcgac
    30481 aacattccgc tcgatgatcc cttgacattt caactttacc aaaaaggcga taccataggc
    30541 acatttcaat tcgaatcgga aggaatgcgc atgtacctga gggaactgaa acccgagcat
    30601 atcgaagact tgattgccat gaacgcttta tatcgtcccg gccccatgca atatatcccc
    30661 atgtatatca atcgaaaaaa aggacgcgaa aaggttacct atcctcatga catgcttcgc
    30721 aacattctgg aagacactca gggaattatg atttatcagg aacagattat gcaggtagca
    30781 caaaaaatgg gaggttttac tctcggaagc gccgatattc ttcgcagagc gatggggaaa
    30841 aaggacccgg ttgtaatggc acaacaaaag gaaatcttca ttcaaggtgc gttaaataaa
    30901 ggagttactg tcgaaacagc ttccgaagtc tttgaaacta tgctcaagtt tgcccaatat
    30961 ggcttcaacc gatcacactc tactgcctat tctattctgg catatcaaac agcttatctc
    31021 aaggcaaatt ttcctgcaga atacatggct gcagtcctaa cccataactt gaacgacatt
    31081 aaaaaaataa cctattatat cgatgaatgt cagagaatgg gaataaatgt actggggcct
    31141 gatgtgaatg agagtcaact aaactttttt gtaaataaaa aaaatgaaat aaggtttggg
    31201 cttactgcca tcaaaaatgt tggcgaaaat gcagctaatt ccatcataaa agaaagagaa
    31261 aatggacttt atacttcact cgaggatttc atttttcggt taaatttgaa agccatcaac
    31321 aaaagaacac tcgagtcatt gattatggct ggtgcactcg atggcttcca aaacattcac
    31381 agagctcaat tctttcacaa aagccgggaa gatgaacccg ctttcatcga aaagctctta
    31441 cgacatgctt ccttgtttca agcaagaaag caatcgagcc agatgagttt gtttggggaa
    31501 ttcgaagaat ttgctcttcc taaaatcgag ctaccggcat gcgaaccttg ggatcagctt
    31561 acaaggcttc attacgaaaa agaagtaacc ggtttttata tctcaggcca tcccctggat
    31621 ttattcaaat tggaaatcga caatttatgc acccatagta tcaaggattt taaaaatgac
    31681 ctcgaagctc acgccggcaa ggaaatcatt ttcgcagcta tggtctcgga agccaaggag
    31741 agatatggaa aaaacaataa aatttactgc tcgatagttt tggaggattt tgatgaaaca
    31801 tactctgtta ccttgttttc agaacaatat ctcaaataca agcacttatt gacgccagga
    31861 agcaatattc tgataagagc tacagcaaag caacgtttca atggttcttc ctacgaattg
    31921 ggaataaacc aaattggttt actttcggat gcgctcgata actgggtgga aaaaactcaa
    31981 gttttcattc ccttgccaat aatggatgac catttaattc aacagcttga ccaatcaatt
    32041 cgagaaaacc ccggtacctc aaccgtatct ttcacggtat tcgatccagc agatcagcta
    32101 tcgctaaatt tcgaaaacga taacttgctt atcaatacat caggctttgc cagagaattg
    32161 aagaaaatcg acggtatttt tttcaaattt ttcaaaaaaa aatactggtc tgattcactc
    32221 tgagagaaag aattaatttt gtcatccatc aacaaaaatt tacaattatg gctaaaattc
    32281 ttacagatca gaattttgaa gaagtggttc tgaaatcaga taaacctgta attgtcgact
    32341 tatgggccga atggtgcggt ccatgccgaa acttgtcccc catcatcgaa gatctggcca
    32401 aggaatatgc cggaaaggtt gttgtcggaa aactcgacgt cgataataat ccacaaacca
    32461 ctatccgatt aaaagtcaga aatatcccta caattctatt tttcaagaac ggagaaatag
    32521 tggacaaaca agttggaacc gttcctaaat cggtcctggt caataagatt gaaaagcttt
    32581 tatgattgtt gtccgaaaaa ttgccgttac gaagcaaaag gatgtaataa gtaaggtcta
    32641 cccacacgaa gagcgaatat gccagttatc gatcctttac gttttcagcc cttaaccggg
    32701 ctggaatttt ttgcccggga agtagttgaa ggctttatca ctggccttca caaaagtcct
    32761 tatcacggct tttcggttga atttgccgaa catcgtctat acaataccgg cgaacctatc
    32821 cgaaatatcg attggaaact ttatgcccgg actgataaat tattcgtgaa gcgattcgaa
    32881 gaagaaacga atcttcgctg tcaactggtc atggatacct cggcctcgat gtattatcca
    32941 cctatacagt atcccgatgt tgaacatccc actaaacttc ttttttcgat ttatgctgcc
    33001 gcagctctga tgttcttgtt taaacagcaa agagatgcag tgggcttgtc gatctttaat
    33061 aatgacataa gccaaaccat tcaggctcgc tccagtgcca ttcatatcca ttatttattc
    33121 accgaattag agaaattaat agaatcgcag cctccatctc gtccccacga aagttccatt
    33181 gccgattcac ttcatttgat cgccgaatcc attcacaaaa gatcactggt gatcattttc
    33241 agtgatatgt tcgataaagc cggtgagcca gaattattat tccatgcttt gcaacatctt
    33301 cgacataaca aacatgaagt cattcttttc tatactatcg acaaacagct ggagttaaac
    33361 ttcgacttcg agaaccggcc ttaccgtttt gtggatatag agaacggaaa agagatcaaa
    33421 ctaaatccct acgaactgag agaacgatac atgacttcga tgcagaattt tctggatgaa
    33481 ttaaaatttc gttgcggtca atatcatatt gatttggtcg aagccgatat tcacgaagga
    33541 ttcaaccaga ttctactccc ttacctaatt aagcgctcga gactttatta ggttcgatgg
    33601 acaaaggata atgattgagg atgaaagttg taagttgttt gaattttact acgagttagg
    33661 tggagtgaat ttcacggtag ctggtattta ttgctgtatc actcaggcat atggctatat
    33721 tccccataag tcacccttcc ccatccacca cgttcatcac ctttcacata tcagattcca
    33781 aaattctttt taacaaacaa ataaatgaaa tttactgttt catcgatacc aagggtggat
    33841 gaatttatgc agagatggta tgaatcggca acgccccaat gtttattcgt aaaataattg
    33901 taataatctg ctcttttttt atccattttc ttaataaaat ctttggcttt attcacctga
    33961 taatgataag tctccgcaat ggtttttatt ctatcttcaa aattggcgca aataaataca
    34021 ttcaaacaac gttcgcagtc tttcaacaca taatctgcgc aacggccaag gaagatacat
    34081 gatttttgtg cagctaattc acgaatcaca tcactttcga tcacaaataa tgattcattg
    34141 gttaaataat attgtgcata gatttcatcg ggtataggac tgctcattcc cagcaatccg
    34201 cctatcagac tacgacttcc tttttcatca aattgctcga aaatctcgct tcgtaaaccg
    34261 ctttttctgg aagccatttg gataagttcc ttatcataga cgggcagttg taagatttgg
    34321 gcaagttttt cgctaatttt cttccccccg cttccaatct gacgtcctat acagatgtaa
    34381 tatttttcat ccataattcg aagatttgaa ctaacaaact tacataaaaa aaataatttt
    34441 catacgccgc acaacaaaaa tcaaggcca
//
LOCUS       DAOORU010000009        33968 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_1489_length_33968_cov_6.316486, whole genome shotgun sequence.
ACCESSION   DAOORU010000009 DAOORU010000000
VERSION     DAOORU010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33968
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1489_length_33968_cov_6.316486"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLF20_01465"
     CDS             <1..358
                     /locus_tag="PLF20_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOX79712.1"
                     /translation="IILAGFLWKIPDFLIQQLAGRILNILPALLPKYGDKVMYGERVH
                     QAVLAAGEMESGISIHRVDEAYDHGEIVFQFKCPVLLEDTPETLANRIHKLEYEYYPK
                     IIEEVIVSDGKRQTTL"
     gene            complement(414..1613)
                     /locus_tag="PLF20_01470"
     CDS             complement(414..1613)
                     /locus_tag="PLF20_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HOX79713.1"
                     /translation="MKTIADQIKEISENIHPSLIQIRRQLHQYPELSGEEFGTSAFIS
                     HTLNEWGIDQRTGIAGTGIMAILRGKIPGNACVALRADMDALPIDEQNEVEYCSKNPG
                     IMHACGHDVHVSSLLGTIKILQQLSDEYPGIIKCIFQPSEEKYPGGAIRMMEDGILQN
                     PEPLAIFGQHVCPSLPYGKVGFKPGKYMASTDEIYITIKGRGGHAAIPHQNIDPVIIG
                     AQILVALQQLVSRESDPTIPTVLSFGKFVANGRTNIIPDEAHMEGTLRTFDEMWRHEI
                     HSRIGRVVSNMAHAFGATCEVFIDHGYPYLQNDESLTQAAIKNAQEFLGNENVVELDL
                     RMTAEDFAYYAQKIPGCFYRLGVATTNLPFPPLHSAHFDVDENCLLTGPALMAWLALK
                     KLNPSLK"
     gene            complement(1625..3463)
                     /locus_tag="PLF20_01475"
     CDS             complement(1625..3463)
                     /locus_tag="PLF20_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX79714.1"
                     /translation="MLKSLKVLRYAIPYWSSALLNIIFNLLSVIFSLVSFALFIPVLQ
                     IIFDRTKRLVPGVDFNPDWHSIHDTDSLKQFFYGSTSQMIEIQGEMKALLYISILIVV
                     LYLLKNLFRYLAMFFLATVRNGVVKDLRNALYYKALILPLSFYTQQRKGDLMARMGGD
                     VQEVEWSIMSSLEMLFRDPITIIVYLITLFIIQPVLTGFVLVLLPISGFLIAQVGKSL
                     KRTSQKSQKKMGYLMSLIEETLSGIRIIKAFNAIDESREHFEKENHAYTRMMTRLYRK
                     RDLASPLSEFLGIVVVVVVLMFGGRLILSQNSSITAEVFLVYIGIFTQLIPPAKSIVQ
                     AFYNIQKGAASVDRINQILKAEEVIVEKPNAFPKKSFEQRIEYRNVSFRYEEELVLRN
                     INLIIEKGQTVAIVGQSGSGKSTLVDLLPRFYDCTEGCILLDGIDIRDYRIDDLRGLM
                     GIVSQETILFNDTVFNNIALGMKKVSQHEVEKAAKIANAHEFIMQMPEGYQTLIGDRG
                     TKLSGGQRQRLSIARAVLKNPPILILDEATSALDTESERLVQQALDKLMENRTSIVIA
                     HRLSTIRFADMIVVLHQGEIVEKGKHETLYTQGEFYRKLCDMQSFL"
     gene            3771..5003
                     /locus_tag="PLF20_01480"
     CDS             3771..5003
                     /locus_tag="PLF20_01480"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOX79715.1"
                     /translation="MTENKPGKENPGGFSDQNDSGNGHEKRLRKPLFSTSYFHEENNN
                     STSSESEDESKLRKRPPRKRIIVKPASKSTSEQIHTTQGEEKSFEDPYYGRESSGGGW
                     TGHPYEPQTQYPEKKKPLRGKAFQPASKNKSPYSKGKFSVRKSKIKKSSPAHTRLLKK
                     PKRERAPFIPAKFEGPVRLNRFIAAAGVCSRREADRLIEAGEIMVNEQVVTTLGSRVN
                     QGDKVMYKGKLLRTENKYYILLNKPKGYVTTMEDPAQRETVMELVKDACEERIFPVGR
                     LDRNTTGLLLLTNDGELAKKLTHPTHRIRKIYEVELDRPLTKSDMMRIASGIQLEDGV
                     AEVDAIAYVGKGEDRTKIGIELHSGKNRVVRRIFEKLRYHVKKLDRVAFGTLTKKNLP
                     RGHWRYLTEHEVNFLKML"
     gene            complement(5087..6604)
                     /locus_tag="PLF20_01485"
     CDS             complement(5087..6604)
                     /locus_tag="PLF20_01485"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HOX79716.1"
                     /translation="MNHEERFKNIIAHAKEYGFIFPSSEIYDGLAGVYDYGQNGVELK
                     NNIRQYWWKAMVQFHENIVGLDSAIFMHPAIWKASGHLDKFNDPLIDNKDSKKRYRAD
                     NLIEDHMAKIESKIAKEIEKAAKKYGDSFDEEQFVNTNRRVLEYKKQLQEIHHRFNTA
                     MDKNDLIELRQLILDLGIVCPVSGTRNWTEIRQFNLMFNTAMGSTSEDATTVYLRPET
                     AQGIFVNYLNVQKTGRMKIPFGIAQAGKAFRNEIVARQFIFRMREFEQMEMQYFIRPG
                     TELTWYEFWKEERLKWHLSLGFPREKYRFHDHEKLSHYANAACDIEFEFPFGFKELEG
                     IHSRTNFDLNAHQQYSGKKIQYFDPEINESYTPYVVETSIGLDRMFLAIISHAYHEET
                     LDDGSERVVLRIPPILAPYKAAVLPLVAKDGLPEKALEIMDILKYTFMCQYEEKDSIG
                     RRYRRQDAIGTPYALTIDHQTLEDDTVTIRERDTMLQDRVIIDKLPEIIQGKLQI"
     gene            complement(6730..9117)
                     /locus_tag="PLF20_01490"
     CDS             complement(6730..9117)
                     /locus_tag="PLF20_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOX79717.1"
                     /translation="MEKHWPHLHFEVKLPKKWVITCCLLLFTIFLQAQIKYSRVEIEK
                     TYLTELAKQGIAVEDGVFTEDNKLMIELSENELKQLTQIGIPFEVVIENVTEFYIERN
                     EKRIKNIQNIHDIPVPTGFSLGSMGGYCTLSEIYMHLDSMHARYPNLINARQSLGSLT
                     TQQGRQLFWVKISDHPKMDESENRILFTALHHAREPIGMQQMLFFMYYLLENYDSNPY
                     IHQLIDTTEIFFIPCVNPDGYEFNHLVSPNGGGMWRKNRRLNPDNSYGVDLNRNYGYM
                     WGYDDLGSSPVPSSETYRGPSAFSEPEIQMIRDFAQLYDFNLVFNYHAYSNTLLYPWG
                     YITDTTAENPIFKNFAFKLTNYNANAYGPASLMLYLVNGNSDDWYYAGQVNQQRAFSF
                     TPEIGNNMQGFWPSIDQIILLCQDQVEANFLAIRFGSRYGEITQHNKLFFSQNQNFVS
                     FHFKRYGLEEGVTYKVSLLPLSNLIESVGQPVYFNHPELLISYSDSISFSVSKDILPG
                     DEIKILLTLEDDYFTHSDTLTLIFGIPYPIFTDECTTMGNWSSNKWGNNSFVYNSPPS
                     SITDSPVGNYSSNDNTSITSTQEFDLTKAKAAVLSFYALWDTERRYDFVQVFASIDQG
                     QHWTALQGKYSSPSSNPLVMDQPVYQGTNLQWVNEEINLSPFTGQKLKIKFALKSNQF
                     INKDGFYFDDISLQIIDKSTGITPSEQNNQLLYTIFPNPANGALHIRPANPHTAQSVQ
                     VSIYNIFGKLFLRQSFPSSIRRMDVNLENLPSGVYFISIEAGVEQVQWEKLLISH"
     gene            complement(9081..9668)
                     /gene="gldD"
                     /locus_tag="PLF20_01495"
     CDS             complement(9081..9668)
                     /gene="gldD"
                     /locus_tag="PLF20_01495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HOX79718.1"
                     /translation="MFEVLTMAVILTSCHHQPIPKPKGFLRIDLPQKAYKDFDTTFPY
                     AFQYPAYSLIKPARKPVENPYWIDIVFPIFKATVYLSYKPIHDNLQQYTEDSRTFVMK
                     HIPKASSIEEFVVQNPDANVYGLFWLIKGREAASPCQFYLTDSSKHFLRGALYYDFNP
                     NNDSLAPVTNFLISDIQHLAQTLKWKNIGHISTLK"
     gene            complement(9692..11059)
                     /gene="gldE"
                     /locus_tag="PLF20_01500"
     CDS             complement(9692..11059)
                     /gene="gldE"
                     /locus_tag="PLF20_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HOX79719.1"
                     /translation="MELPSIISSFVCGNAIFCRVEVANAPLNVSMGLLAIIILLICSG
                     LISASEAAFFGLSASQIEKIKNSSENYFRRASYLLENPDFLLATILIANNFINVGIVL
                     ISVYVTNLIVSVEDYPLATFIIQSVVVTLIILFFGEISPKIYSTHKKESIIRLMATPL
                     FVLEKLFKPINFVLVKSTGIVDRRLKKREDDISLKDISEAINITKTSSDKHDEARMFK
                     GIVNFGNIQVREIMVPRVDAVAVNDNTPYDELLQIIRQSGYSRIPVYSGSPDSVVGIL
                     YIKDLLKHLNEGKDYPWQRHLRPAYYIPENKKIDTLLTEFQSQKIHIAIVVDEYGGTS
                     GIVTLEDILEEIFGDISDEFDEEEDEKNYVKVDENTYIFDGKILLNDFCRILQIREDI
                     FDEVSGEFDTLAGLILELEGRLPATNEVIFYKNFEFRIESADYKRIKGVRVTYHYDEE
                     EDKKK"
     gene            complement(11267..11683)
                     /gene="ssb"
                     /locus_tag="PLF20_01505"
     CDS             complement(11267..11683)
                     /gene="ssb"
                     /locus_tag="PLF20_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOX79720.1"
                     /translation="MANGINKVILVGHLGRDPEIREFNNGKQATFTLATTESYKKSDG
                     QRVDQTEWHNIVLWRGLAEIAEKYLHKGDMIYLEGKIRRREYEDQGQKKYFYTIQGDN
                     FTILSPKKEGIPQVTEAAEPDIEIPPIEGGTDDLPF"
     gene            11822..13570
                     /gene="aspS"
                     /locus_tag="PLF20_01510"
     CDS             11822..13570
                     /gene="aspS"
                     /locus_tag="PLF20_01510"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOX79721.1"
                     /translation="MLRTHTCGELNTNHIGEEVILCGWVQRTRDFGGMTFLDLRDRYG
                     ITQLVFNMEWDVHLCEAARKLSREYVIRAKGKVTKRESINPKLPTGEIEIIVDEFEIL
                     NESKIPPFTIEDQTDGGEELRMKYRYLDLRRNPLRKNLEQRHALAMETRKYLCEQHFL
                     EIETPYLIKSTPEGARDFVVPSRMNPGQFYALPQSPQTLKQILMVAGYDRYFQIVRCF
                     RDEDLRADRQPEFTQIDCEMAFVDQEDILQVFEGLIIRLFSKVKGIELDSFPRISYDE
                     AMKRFGSDKPDIRFGMEFTEITSRAKGKGFKVFDEAEIIVGINAKKCAQYSRKELDQL
                     TEWVKRPQIGASGLIYIKFSEDGSLRSSIDKFFRQDHLRELSAEFSAKPGDLMLIIAG
                     AKEKTLKALGELRLEMGNRLGLRNPNVYRPLWVIDFPLLEWDEELQRFFAMHHPFTSP
                     KPEDIPLLETNPSLVRANAYDLVINGVEIGGGSIRIHLKELQNRMLRLLGFTQEEAQK
                     QFGFLMDAFEYGAPPHGGIAFGFDRLCAIFGGSDSIRDFIAFPKNNSGRDMMLDSPSE
                     ISQQQLNELYLRINLP"
     gene            complement(13640..14635)
                     /gene="trpS"
                     /locus_tag="PLF20_01515"
     CDS             complement(13640..14635)
                     /gene="trpS"
                     /locus_tag="PLF20_01515"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOX79722.1"
                     /translation="METVVSGIRPTGELHLGNYFGAIKNFVKMQEDNRCFFFIADYHS
                     LTTHPTPSNLHYNVRRVLINYLASGLDPEKSTIYIQSDLHEVAELYLLLNMNAYLGEL
                     QRVTSFKEKARKQPDNINAGLLTYPVLMAADIIIHKAHKVPVGKDQEQHLEMTRTFAN
                     RFNRLYHVDYFTEPQAYSSEKDLIRIPGLDGSGKMGKSEGEGNAIFLSDEPEVIRKKV
                     MRAVTDDGPTRPHQKKSEPVQNLFTLLKVVSSPDTVTYFEEQYNTCRIRYGDLKKQLA
                     EDIIQFTTPFREKILQLDADNDYLHKVVTEGAAKARASAQKTIREVREIIGILQF"
     gene            complement(14707..16257)
                     /locus_tag="PLF20_01520"
     CDS             complement(14707..16257)
                     /locus_tag="PLF20_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOX79723.1"
                     /translation="MTRELIIDANSNEVSIALLEEKSLVELHKEKMNNNYAVGDIYLG
                     KVKKLMTGLNAAFINIGYEKDAFLHYLDLGPQVQSLNKLTRQIMGGKHFHADLSDFQP
                     EADIEKTGKISEVLAPNQPVIVQIAKEPISTKGPRVTSEISFAGRYLVLVPFSNRISV
                     SQKIKSQEERNRLKRLVTSIRPKNFGIIIRTVAEGKLVADLDADLKSLMAKWEMVMKK
                     LSEAKPPQKIISEIDRTSALLRDLLNESFNNIYINDPGLYEETRSYIQTIAPDKVNIV
                     KLYKGKLPIFEYFGIDKQIKNSFGRTVSISSGIYLIIERTEAFHVIDVNSGHRAKKNN
                     DQETNALEVNLEAATEVARQLRLRDLGGIIVVDFIDMVNPQNRKKLYEHLKEEMAKDR
                     AKHTILPPSKFGLIQITRQRVRPETNVEVLELCPACMGTGQVKPSIILVDEIENKIRY
                     LIHEQNEKQIKMSVHPYVYAYLTKGFPSIQLRWVFKFKKWISISANKSFHMIEYHFFN
                     NKGDEIRI"
     gene            complement(16519..16803)
                     /locus_tag="PLF20_01525"
     CDS             complement(16519..16803)
                     /locus_tag="PLF20_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOX79724.1"
                     /translation="MTKAEIVAEIANKTNVEKIAVQQIVEAFMDSIKKAMINGENVYL
                     RGFGSFVIKRRAEKTGRNISKNTTIIIPAHNIPSFKPAKSFVNEVKNNVK"
     gene            16929..18020
                     /gene="mutY"
                     /locus_tag="PLF20_01530"
     CDS             16929..18020
                     /gene="mutY"
                     /locus_tag="PLF20_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOX79725.1"
                     /translation="MNNPTPFAESLLSWYANNARDLPWRIDVSAYTTWISEILLQQTR
                     LNQGIIYYHRFIERFPDISSLAEASEEEVLKLWQGLGYCSRARNLHEGVRQIIENYHG
                     QIPSSFQELKKIKGIGSYTAAAISSIVFHQAVPAIDGNAIRVFSHFMGINDPVDTSSG
                     RISIEKAAYSLMDQQRPGDFNQAVMELGALVCTPKRPACFSCPLINGCIAYREDKIDL
                     IPSKHRKTRVKERYIFYLVILSHAKNNMQVIIKKRDQNDIWKGLYDFPSLEFDGPADK
                     TAVIESENLKIDFPYLSPAELKFTKGPSKHILSHRTLCLNFLVFESTMLPAIKNKKHR
                     VISIDEAIHLPLPRPIEKFLEELIVGQIP"
     gene            complement(18207..19235)
                     /locus_tag="PLF20_01535"
     CDS             complement(18207..19235)
                     /locus_tag="PLF20_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HOX79726.1"
                     /translation="MTSFDPNAIGIPNGNFLGLPYSNEEADIIIIPVPWDVTTSYHPG
                     TCQGPEAIIKASTQVDLFDFFLEQAWKNRIATLPLSEDILQLNEQLRPLAQQVIHLLE
                     TGMNANNPQILPLLEKINLGCEKMNQWVYKTSYYWISKGKTACVLGGDHSTSLGLMRA
                     LTKQYDGFGILHIDAHADLRKAYEGFIFSHASSMYNALELKSLEKLVQVGIRDVCEDE
                     MMLGYHDKRIKQYDGYLLAFETLNGKPFSEICEEIVKHLPSKVYVSFDIDGLNPELCP
                     HTGTPVPGGLSFDQVVLLLHILATSGRQIIGFDLVEVAPGSDDEWDANVGARILQKLC
                     NLTRLSIP"
     gene            19865..20575
                     /locus_tag="PLF20_01540"
     CDS             19865..20575
                     /locus_tag="PLF20_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOX79727.1"
                     /translation="MPAQIISLLDDFLSLIYPRLCYACGNSLYLNEAVICNHCLATLP
                     LTYSHLSKDNPVARTFWGRIPVEAAAARYYFRKGGPVQRLLHNLKYNHAPEIGKFIGL
                     IYGRELLESEVFRSVDLIIPVPLHFEKLKKRGYNQSAMFGQGLSSSMKIPIDDESLFR
                     TYFTETQTRKSREKRWENVKSVFALKENVSLRGKHILIVDDVITTGSTIEACANRLLE
                     MEGVRISVAAIAFPSHIF"
     gene            complement(20739..21239)
                     /locus_tag="PLF20_01545"
     CDS             complement(20739..21239)
                     /locus_tag="PLF20_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HOX79728.1"
                     /translation="MKKNIEQAIIEQIQKEEHSSRLYLQMAIWCEINGFPGAAAFLYK
                     HSDEERLHQLKFIHFLNDREGMALLKPLETPQSDYPSLKEMFEKILEHEKFISASINQ
                     IYGLTLQEKDYTTASFIHWFINEQIEEETLVRTILDKMKLAGDGQNRGLYLIDKELGV
                     MAASGH"
     gene            complement(21276..22154)
                     /gene="atpG"
                     /locus_tag="PLF20_01550"
     CDS             complement(21276..22154)
                     /gene="atpG"
                     /locus_tag="PLF20_01550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HOX79729.1"
                     /translation="MSNLKEVRLRIESVKNTQQITNAMKMVAASKLRKAQNAILSLRP
                     YAQKMSELISTLYSNYSENLSSPFFEQHETDKVLLVSLASNRGLCGAFNSNIIKQIKY
                     LTETSFEQQYGLNHLKLISIGKHVSDILINKKYPVLQIKDNLIEKVTWEGVKLLADEL
                     IELWTKHEFDRIVLVYNQFINSATQVVTTETFLPLSYDENKKTRNIDYIFEPSSETII
                     EELIPHYLHIQLYKTFLDSFASEQGARMTAMMIATDNADVILKDLTLSYNKARQAAIT
                     KEILEIVSGSNALNKV"
     gene            complement(22168..23748)
                     /gene="atpA"
                     /locus_tag="PLF20_01555"
     CDS             complement(22168..23748)
                     /gene="atpA"
                     /locus_tag="PLF20_01555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005787491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HOX79730.1"
                     /translation="MVEIKPAEVSAILRQQLAGHRTTAELNEVGSVLQVGDGIARIYG
                     LKNAQSGELVEFENTGVKAIVLNLEEDNVGVVLLGESNLVKEGDIVKRTGEVTSVEVG
                     EGMLGRVINTLGESIDGKGDIPGPLYRMPLERKAPGVIYRRPVNEPLQTGIKAIDAMI
                     PIGRGQRELIIGDRQTGKSSIALDTIINQKEFYEKGEPVYCIYVAVGQKGSSVANFVK
                     TLEDFGAMPYTIVVMATASDPAAMQFYAPFTGAAIGEYFRDTGRPALIIYDDLSKQAV
                     AYREVSLLLRRPPGREAYPGDIFYLHSRLLERAAKIIDSDQIAAQMNDLPESIRDKVK
                     GGGSLTALPIIETQAGDVAAYIPTNVISITDGQIFLETNLFNSGIRPAINVGISVSRV
                     GGNAQIKGMKKIAGTLKIDQAQYRELEAFSKFGSDLDPATIAILEKGKRNVEILKQGL
                     HQPLPVEEQIAIIYCGTHNLLKNVPVNRIHDFETEYLNLLSEKYPDILQTIQTGQFPD
                     EVANKLEEIVAKLVKRYE"
     gene            complement(23763..24329)
                     /gene="atpH"
                     /locus_tag="PLF20_01560"
     CDS             complement(23763..24329)
                     /gene="atpH"
                     /locus_tag="PLF20_01560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HOX79731.1"
                     /translation="MKIIRHKVLAQRYALALYEIADSSGLLEEVFEDMQLVDRVNIEN
                     PELKRVWISPIIPPSKKRNILIALFKDKINDISLTFLTLVTDKAREIILHEIVLSFIE
                     IYRQIHHIYEVRIKVAAPMNEENKARIIEKLKPVIKGTIELEVEVDPRLIGGFQLFFD
                     DYLYDASLLKQLKELKKEFSENLFVTQF"
     gene            complement(24344..24838)
                     /gene="atpF"
                     /locus_tag="PLF20_01565"
     CDS             complement(24344..24838)
                     /gene="atpF"
                     /locus_tag="PLF20_01565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HOX79732.1"
                     /translation="MELITPEVGLIFWMTLSFGILVFILGKFAWPTILKALRNREKNI
                     EEALQLAEKARQEMNQLMATNERLLNEAKQERDALLADARKLADIILEEARQRAHTEY
                     DHILALARQAIKNEENAALINLKNQIGALSIEVAEKLLREKLSGEEKQNEYLQRLINE
                     ITLS"
     gene            complement(24942..25202)
                     /gene="atpE"
                     /locus_tag="PLF20_01570"
     CDS             complement(24942..25202)
                     /gene="atpE"
                     /locus_tag="PLF20_01570"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HOX79733.1"
                     /translation="MTLLSLILQAARDLSPFAKIGAGLGASIAAIGAAIGIGNIGARA
                     LEAIARQPEAANDIRSSMIIAAALIEGVAFFAVVVCMLILFV"
     gene            complement(25230..26318)
                     /gene="atpB"
                     /locus_tag="PLF20_01575"
     CDS             complement(25230..26318)
                     /gene="atpB"
                     /locus_tag="PLF20_01575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOX79734.1"
                     /translation="MIKKLFFFIFAIFFPFTLVKAENSPVKAAEFKPGEMIIEHITDA
                     HEWHILSIGNTHISIPLPIILWHEGKLHIFCYSKFHHGQSDYGGFRYMTDGAYKNKII
                     YVGNNSLGNENKSPTIPLDFSITKNVASLFLSVIALIIIFLTVARAYKRNEKHAPKGL
                     QSLLEPIIIFVRDDIAKSSLGEKHYERFTPYLLTVFFFIFFNNLLGIVPFFPGGANVT
                     GNITVTMMLALFTFLITNLSGNRHYWQDIFNTPGIPWWLKIPIPILPLIEFLGLFTKP
                     FVLMVRLFANMTAGHIIVLGFMSLIFVFGNINPAFGYGISLISILMSIFISLLEILVS
                     FLQAFVFTLLSALYFGMATEEIENSNHK"
     gene            complement(26478..27902)
                     /gene="pyk"
                     /locus_tag="PLF20_01580"
     CDS             complement(26478..27902)
                     /gene="pyk"
                     /locus_tag="PLF20_01580"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOX79735.1"
                     /translation="MRTKIVATLGPASFDKSVIERMTKAGVNVFRLNFSHGDHNILRQ
                     IIHYIRELGPAYHHRLAILGDLQGPKIRIGEVENNAINLRQGQEIQLGGGSGLSKEDI
                     LWINYPALTEDVKPGDQVLLDDGKLSLIVKEKLPSGFLRAEVLVGGILTARKGVNLPD
                     SHLTLSALSEKDLTDLRFAYQNQLDWIALSFVRSSEDILKLRENIQFLGRNWNPGIIA
                     KIEKPEALKNFDSIIEVSDALMVARGDLGVELPYEQVPAVQKLVIHKSIAQSKPVIVA
                     TQMLEGMINNTRPTRAEINDVANSVMDGADALMLSAETSMGQYPVEAVEAMRRIIDET
                     EKIGEIHNRKPENSFTNPSRLISDAVIEAAIHLSEKTEAKAILTYTLTGYTALRLASY
                     RPKAQIFIFARSPRLIRRLSLVWGITGFVQQNLHYTDDNLDLMVQILLQNNAVNPDDI
                     LVKITGFPMKERATSNMVVVTRVK"
     gene            complement(28198..29190)
                     /locus_tag="PLF20_01585"
     CDS             complement(28198..29190)
                     /locus_tag="PLF20_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HOX79736.1"
                     /translation="MNTENTMKFRKIVIVGSGSIGTAMAQALASNDELDTTILSIEPE
                     VIDEINHFHRNSKYFPGFRLASEVKATADKSVLREADLLFLALPSVAVIPYIRENSSL
                     LPPHVVLINLAKGFDQGGQTISEGLIKEFPFRAGAMKGPSFAKEMINGSPTAFTVGCH
                     DNTLRPLFEELFANTQVYLDFSKDIKGVELLSILKNIYAIAVGIMDAQFNTANMRFLF
                     LTRAFNEMRSIHILFGGKKTTMFKYCGFGDFGLTALNDLSRNRTLGLLIGKGYFTEQI
                     SEKVLLEGKMAVNIFCGQIIHSKHLNTFPIMSGLYHIFSNQAEVPEFVEQMLKK"
     gene            complement(29187..30668)
                     /locus_tag="PLF20_01590"
     CDS             complement(29187..30668)
                     /locus_tag="PLF20_01590"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOX79737.1"
                     /translation="MFDKFRLSVKQVFIYSLGNIFSKLVGFVLLPLYSQHISISDYSS
                     LILIEPIWQLLSAVLSFSLPTALLRWLAPEKDPKRQGSMVFTTLMALVFILLAFNLLA
                     FPFNAWLNHRGVYGDSQFKLYLDISFLLVTFDVLNLLVLSLLRFHEKPWQYIILNTLK
                     LTVNLGLNVYFIVGRGMGVEAILLSQLIASFLLNVFSFPFLLKHLVPKFESGPLKEML
                     RYGFPLIFTSISAIILSLGDRYVVQHFLTRSDTGVYGIGYKFGGVINMFIIQSFQLGF
                     LPIAYKMLEDAEAKRFFSRIFTYYFGILMISAFALSIFSHEIVAIFTLGNVDYMRAYV
                     VIPLISFGFVLKGAQTYFSFGLHYVKKTKFNAWIVMLCAIFSIGMNILLVPKMGIYGA
                     ALIMLVSNGLLAIFFYFFSQKFYYIHFEGIRLAKVLITGLSLFAIGYFVPVGNLLASI
                     LFKLLLLIAFPLILYLLKFFNVDEINRLRQIFRSLYIKKSFKE"
     gene            complement(30669..31445)
                     /locus_tag="PLF20_01595"
     CDS             complement(30669..31445)
                     /locus_tag="PLF20_01595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX79738.1"
                     /translation="MMDNPIIYEKVDWEILTNKQLGPKIKKIIENIPSDVQSIIDIGC
                     GNGVITNELAKKYQVLGVDRSKSALQYVTSEKLEASCDNIPLPSQNFDMVFSSELLEH
                     LPNEIFQKTIQEFKRISKKYIYITVPNRENPDKLMIRCPECKYIFNRPNHLRSFKSED
                     FTVLFPEYKKIAEFTFGKPVRYYHPALLRLKRRWIPSISWIPYFWIDQNNRTAICPQC
                     NHKFYYFYKFHPLGFLIDGINVIISPKRPYWLMVLLEKNS"
     gene            complement(31442..32695)
                     /locus_tag="PLF20_01600"
     CDS             complement(31442..32695)
                     /locus_tag="PLF20_01600"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOX79739.1"
                     /translation="MGWENFKKNSGNNFTSFAALMKILMLLPGEFPPDIRVENEAMAL
                     AKAGHEISIACLTRKELPSDEFFKGKVIIKRFKIPALRYKSSIATLTLPFYFNFWRNE
                     LNKLLLKNKYDAIHVHDLPLAQIGFEVSKKLNAKFVLDLHENWPGLLKISEHTQSFVG
                     KIFSHDQQWRNYEKRMCNLADKVIVVVEEAQKRLENLGIEHNKITVVSNTLNIESFDK
                     LPKYKVNTSNTNNFNIFYGGGINKHRGIQIVIRGIALLNDHFSDFNFDIVGDGKYLEK
                     LIKLSVTLNISSRIKFYGYLPFGEMLAILQNANVAVIPHLKSEHTDTTIPHKIFQYIY
                     AGIPILSSDCNPLRRIIEETHSGIIYKNDSPTDFAENLIELKNLISLGKFNPEYGRTF
                     IVNKYNWDKDAQKLIQLYSEFDQTK"
     gene            complement(32635..33729)
                     /gene="wecB"
                     /locus_tag="PLF20_01605"
     CDS             complement(32635..33729)
                     /gene="wecB"
                     /locus_tag="PLF20_01605"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOX79740.1"
                     /translation="MKKVLSVVGARPNFIKIAPIHKAFREVESLCEHKICHTGQHYDT
                     LMSDIFFDELDIPTPDFFLNAGGGTHAKQTATIMLRFEKILQQYQPDIVLVPGDVNST
                     LACSLTAVKLGIKVGHIEAGLRSFDRSMPEEINRIVTDAISDYLFVTENSGLRNLKYE
                     GVADEKIFFVGNTMIDNLMQNLEKISNKHFFSSLNLNPKEYIVTTFHRPSNVDSLGNL
                     NELVKFLNEVSKEYDVVFPIHPRTLNNFKKFHLLEKINKNVHLIEPLGYINFLSLVID
                     SKGVVTDSGGIQEETCFLKIPCITVRNSTERPVTIEMGTNQLVGSNFDAALKAIEVFN
                     NSLQNTMIPPLWDGKTSKRIVEIILHHLQP"
BASE COUNT         9964 a         6608 c         6850 g        10546 t
ORIGIN      
        1 tatcattctt gcaggttttt tatggaaaat tcctgatttt ctcattcaac aattggctgg
       61 gcgcattctt aatattcttc ctgctctttt gcctaagtat ggcgacaaag ttatgtatgg
      121 cgaaagggtt caccaggcgg ttttggctgc tggagaaatg gaaagtggca ttagcattca
      181 tcgtgtcgat gaagcgtatg atcatggaga gatagtattt cagtttaaat gccctgtttt
      241 gctagaagat acgcctgaaa cccttgcaaa tagaatccat aagctggaat atgaatatta
      301 tccgaaaatt atcgaagaag taattgtctc agatgggaag cgccaaacca cactttaata
      361 ttccttgaga ttcttgttaa ttttttgagg tataattggt taatcaggca catttatttc
      421 aacgaaggat ttaatttttt caaggcaagc catgccatta aagctggtcc agtgagcagg
      481 caattttcat ctacatcgaa atgagcactg tgcaagggag ggaagggaag attggtggta
      541 gcaacgccaa gtctgtaaaa acatccaggt attttttgag cataataggc aaaatcttct
      601 gccgtcatcc ttaaatcaag ttccacgaca ttctcatttc ccaaaaattc ttgggcattc
      661 tttatagctg cttgggttaa tgattcatca ttttggagat agggatatcc gtgatcgata
      721 aatacttcgc aggtggctcc gaaagcatga gccatattgc tgacaactct tccgattcgt
      781 gaatgaattt catggcgcca catttcgtcg aaggttcgca aagtgccttc catgtgggct
      841 tcgtcgggaa tgatgttggt tcggccatta gcaacgaatt ttccaaaaga aagcaccgta
      901 ggaatggtgg gatcggattc tcggcttacc aattgctgga gagctactaa aatttgtgca
      961 ccaataatga caggatcgat gttttgatga ggtatggctg cgtgtccacc tcttcctttt
     1021 atggtaatat aaatctcgtc ggttgaagcc atgtattttc ccggcttaaa tccaactttt
     1081 ccataaggca atgaagggca tacatgctgc ccgaagatgg ccaatggttc gggattttgc
     1141 agtatgccat cttccatcat acgaatagct cctccggggt acttctcttc cgaaggttgg
     1201 aaaatacatt ttataatgcc tggatattca tccgataatt gttgcagaat ttttatggtg
     1261 ccaagtaggg aacttacatg tacatcgtgc ccacaggcat gcattatgcc cggatttttc
     1321 gaacagtatt ccacctcatt ttgttcgtct atgggcaggg catccatgtc ggcacgcaag
     1381 gctacgcaag catttccagg aattttccct cttaaaatgg ccatgattcc tgtgcctgct
     1441 attcctgtgc gttgatctat gccccattca ttcagggtgt ggctgatgaa ggcagaagtt
     1501 ccaaattcct cgcccgatag ttccggatat tgatgaagtt gccttcttat ctgaatgaga
     1561 gaaggatgaa tattttcgga aatctccttt atttggtcgg caatagtctt catgtttcga
     1621 tttattatag aaacgactgc atatcacata attttcgata gaattcgcct tgggtatata
     1681 gcgtttcatg ctttcctttt tccactattt ctccctgatg gagcactacg atcatatcgg
     1741 caaaacgtat ggttgaaaga cggtgggcaa ttacaatcga tgtacggttt tccattagtt
     1801 tgtcgagagc ctgctgaacc agtcgttcac tttcagtatc gagagcggat gtagcttcat
     1861 cgaggatgag gatgggagga ttctttaaaa cggctctggc tatactcagg cgctgccgtt
     1921 gtccacctga aagttttgta ccccgatcac caattagagt ttgatatccc tcaggcatct
     1981 gcatgatgaa ttcatgagcg ttagctattt tcgcagcttt ttctacctca tgctggctta
     2041 cctttttcat cccaagggct atattattga aaaccgtatc attaaataat atggtttcct
     2101 ggctgacaat tcccatcaaa ccccttaaat catctatccg ataatcgcga atgtcgatac
     2161 catccagaag tatgcagcct tcagtacaat cgtaaaaacg aggtaaaaga tcgaccaagg
     2221 tggatttccc actgcccgat tgcccaacga tggcaacggt ttgtcctttt tcgataatca
     2281 agttgatatt acgcaaaacc aattcctctt cgtatctgaa ggaaacgttc cgatattcga
     2341 ttctctgttc aaaggatttc ttgggaaatg cattgggttt ttcgacaatc acttcttctg
     2401 ccttcaaaat ctggttgatt ctatcaaccg aagcagctcc tttttggata ttgtaaaatg
     2461 cctgtacgat agattttgct ggaggtataa gttgagtaaa aattccgata taaaccagaa
     2521 atacttcggc cgtaatggaa gaattttgag aaaggatgag tcttccacca aacatcaaaa
     2581 cgaccaccac tactactata ccaaggaatt cactcaaggg tgaagccaaa tcacgcttgc
     2641 gatacaatcg ggtcatcata cgtgtgtagg catgattttc cttctcgaaa tgttcccgtg
     2701 attcatctat tgcattaaaa gccttaatga tacgaattcc agatagggtt tcttcaatca
     2761 acgacatgag atatcccatt tttttctggc ttttttggga tgttcgtttt aagcttttcc
     2821 caacctgagc aattaaaaat ccgctgatgg gaagcaaaac cagtacgaaa cccgtcagca
     2881 ccggctgaat aataaaaagc gttattaaat aaacgataat ggtaatggga tcgcgaaaca
     2941 acatttcgag cgagctcatg atcgaccatt ccacttcctg aacatcgccc cccattctgg
     3001 ccattaaatc acccttgcgc tgctgagtgt agaacgataa gggaagaatc aaggccttgt
     3061 aatataacgc attgcgaaga tccttcacga ccccgttgcg taccgtggct aaaaaaaaca
     3121 tggccagata gcgaaacaga ttctttaaaa ggtataaaac cacaatgaga atggaaatat
     3181 atagaagggc cttcatctca ccctgaatct ctatcatctg gctcgtactg ccgtaaaaaa
     3241 actgcttgag tgaatccgtg tcatgaatac tatgccaatc gggattgaaa tcaacaccag
     3301 gcaccaatct tttggttctg tcgaaaataa tctgtaatac gggtatgaac agagcaaatg
     3361 ataccaaact aaaaattacg gataaaagat tgaatatgat attaagcaaa gccgaactcc
     3421 aatatggaat cgcataacga agaactttta ggcttttcaa cattgtgctt ataataataa
     3481 attgcaaagg taaaatgaac tttcattcat agatgaacct ttcattttaa ttgccagact
     3541 ggcgggttct gggaaagaaa acagggaatg tcgatggaat tttcatgatg cctctttgcc
     3601 gatgtccaaa ataaacacct ggatttacag ctcttcctag gctttgctta tattcataaa
     3661 ataattatct tttgatcttt ttttactgaa ttatggaatt ttgacgtatc tttgcaaaaa
     3721 attaaggtta ctaagttcgt attattttat tttaataatt taattttcag atgactgaaa
     3781 acaagccggg caaagagaat ccgggaggat tttctgacca gaatgattcg gggaatgggc
     3841 atgaaaagcg tcttcgcaag ccattgtttt ccacctccta ttttcatgaa gaaaataaca
     3901 attcaacatc gtcggaatcg gaagatgaat caaaattacg caaaaggcct ccgcgaaaac
     3961 gtattatagt aaaacccgca tccaaatcta catccgaaca aattcatacc actcaagggg
     4021 aagaaaaatc cttcgaagat ccttattatg ggagagaatc ttcaggaggt ggttggacgg
     4081 gtcatcctta cgagccccag acacaatacc ccgagaagaa aaaacctttg agagggaagg
     4141 cctttcagcc tgcttccaaa aataaatcac cttactccaa aggaaagttt agcgtaagaa
     4201 agtcaaaaat taaaaaatca tctccagctc atacacgctt gcttaagaaa cctaaaaggg
     4261 agagagcgcc ttttatcccg gctaaattcg aagggccggt tcgtttgaat agatttattg
     4321 ctgctgcagg ggtttgctcg cgtagagaag ccgatcgtct aatcgaggca ggtgaaatta
     4381 tggtaaacga acaggtggtt accactctgg gatcaagggt caatcagggt gataaagtga
     4441 tgtataaagg aaaacttcta cgaacagaga ataaatatta catcctcttg aataaaccta
     4501 aaggctatgt aaccacaatg gaagatcccg cacagaggga aactgtaatg gaattagtaa
     4561 aagatgcgtg tgaggaaaga atttttcccg ttggccgcct cgacaggaat acgacaggcc
     4621 ttttgctatt gaccaatgat ggagaattgg ccaaaaaatt aacccatcca actcatcgaa
     4681 tccgcaagat ttatgaagta gaattagaca gaccacttac aaaaagtgac atgatgcgta
     4741 ttgcctcggg aatacagctc gaagatggag tggccgaggt tgacgccatt gcttatgtgg
     4801 gaaagggcga agatcgaaca aaaataggaa ttgaacttca tagcggtaaa aacagggtcg
     4861 tacgccgtat cttcgagaaa ttgagatatc atgtaaaaaa actcgaccgc gtggcctttg
     4921 gtaccttaac caaaaagaac ctcccccgtg gacattggcg ttatctcacc gaacatgagg
     4981 ttaatttttt aaaaatgctt tgatcggtaa tgaaagaata atagtttttc tctttgtttt
     5041 tggctctcaa agaaaataat agaatagccc tcactcattt ataatcttat atctggagct
     5101 tcccctgaat aatttcgggt agtttatcta taataacgcg gtcttgcaac atagtatccc
     5161 gctcccgtat ggttacagta tcatcttcaa gagtttgatg atcgatggtg agggcatagg
     5221 gcgtaccaat agcatcttgt ctgcgatatc tacggccaat ggagtctttt tcttcgtatt
     5281 gacacatgaa tgtatacttt aaaatatcca ttatctcgag ggctttttct ggcaatccat
     5341 ccttggcaac caatgggagc acagccgctt tgtatggggc aagaatagga ggaatacgca
     5401 gaaccaccct ttccgaacca tcatccaggg tttcttcatg ataagcgtgg ctaataatgg
     5461 caaggaacat acgatccaaa ccaatggagg tttcaacgac gtaaggggta taactctcgt
     5521 tgatctcagg atcgaaatat tggatctttt tccctgaata ttgctgatgt gcgttaaggt
     5581 cgaaatttgt gcgcgaatgg atgccttcaa gttctttgaa tccaaaagga aattcgaatt
     5641 ctatatcaca ggcagcattg gcataatgtg atagtttttc gtgatcatga aaacgatatt
     5701 tttcacgggg gaaacctaag gacaaatgcc atttcagccg ttcttccttc cagaattcat
     5761 accaagtcaa ttctgtgcca ggacgaataa aatattgcat ctccatttgc tcgaactcgc
     5821 gcatgcgaaa aataaactga cgtgcaacga tttcgttgcg aaatgcttta cccgcctggg
     5881 caattccgaa aggaatcttc atgcgccccg ttttttgaac attcagataa ttgacgaata
     5941 taccttgtgc tgtctcaggc cgtaaataaa cagtagtcgc atcttcacta gtcgaaccca
     6001 tggctgtgtt gaacatcaga ttaaactgtc tgatctcggt ccagttgcgc gtgcccgata
     6061 cgggacaaac tattcccaag tcgaggatca attgccgaag ttctatcaaa tcgtttttat
     6121 ccatcgcagt attgaagcga tgatggattt cttggagttg ctttttatat tcgagaaccc
     6181 tccgattggt attgacaaac tgttcctcgt cgaagctgtc accatatttt ttggcagctt
     6241 tttctatttc ctttgctatc ttgctctcga tcttagccat gtgatcttcg ataagattgt
     6301 cggcacggta gcgttttttc gaatccttgt tgtcgatcaa gggatcgttg aatttgtcga
     6361 gatgacctga ggctttccat atcgcaggat gcatgaagat ggcagaatcg aggcctacaa
     6421 tattttcgtg aaattgcacc atcgctttcc accagtactg gcggatgttg ttctttaatt
     6481 ctactccgtt ctgtccataa tcatatacac cggccaaacc atcataaatc tcactcgatg
     6541 gaaatataaa cccatactct ttggcatggg ctatgatgtt tttgaatcgt tcttcgtgat
     6601 ttatcatgcc gcaaaaatag agcttttcac tgttctgtta aatttttcaa cacaagctta
     6661 ttcatttcga ctaattaacg aaatgcacgg gttgacaata cgatcgaggg acaataataa
     6721 ttacaaggtt tagtgcgaaa taagaagctt ctcccattgt acttgttcaa cacctgcctc
     6781 gatacttata aaatagactc ctgaaggcag gttttcgagg tttacgtcca ttcggcggat
     6841 tgaagaggga aaagattgtc gtaaaaataa tttgccaaaa atattataaa tgctgacttg
     6901 gacactttga gcggtatgag gattggcggg gcgaatatgc agtgcaccat tggcaggatt
     6961 gggaaatatg gtgtaaagga gttgattatt ttgttctgat ggagttattc ctgtggattt
     7021 gtcgataatt tgtagtgaaa tatcatcgaa atagaatcca tccttgttaa taaactgatt
     7081 tgatttgagg gcgaatttga tttttagttt ttgccctgtg aaagggctta gattgatttc
     7141 ttcattcacc cattggaggt tggttccctg ataaacgggt tggtccatca cgagaggatt
     7201 cgaactaggt gaagaatatt ttccctgtaa agcagtccaa tgttgacctt ggtcaattga
     7261 tgcaaacacc tgaacgaaat cataacgacg ttccgtatcc cacagagcat aaaacgaaag
     7321 cacggccgcc ttggccttgg ttagatcgaa ttcttgagta gatgtaatag aagtattgtc
     7381 attgctcgaa taatttccaa caggcgagtc ggtaatggaa gaaggtggac tgttgtaaac
     7441 gaaactgttg ttgccccatt tgttcgatga ccagtttccc atcgttgtac actcgtcggt
     7501 aaaaattgga tagggaatac caaaaatcag ggtaagcgtg tcggaatgcg tgaaatagtc
     7561 atcttctaac gtcaaaagaa tttttatttc atcacccggt aaaatatcct tcgagacaga
     7621 aaaggaaatg ctatcagaat aagagatgag caattccgga tggttgaaat aaacgggttg
     7681 acctactgat tctatcagat tacttaaggg tagcaggcta actttatagg taactccttc
     7741 ttcgagtcca taacgtttga aatgaaaact gacaaagttt tggttttgag agaaaaacaa
     7801 tttgttgtgt tgagtaattt caccataccg gcttccgaaa cgaattgcca gaaaatttgc
     7861 ttctacctga tcctggcata gaagaatgat ttggtcgatg ctaggccaaa atccctgcat
     7921 attgttgccg atctctggag tgaacgaaaa ggccctttgc tgattcacct ggccagcata
     7981 ataccaatcg tccgaattac cattcaccag ataaagcata aggcttgccg gtccataagc
     8041 attagcatta taattggtaa gcttgaaggc aaagttttta aagatagggt tttcggcggt
     8101 tgtgtctgta atgtagcccc agggatataa cagggtattg ctatatgcat gataattgaa
     8161 aacaagatta aagtcgtaaa gttgagcgaa gtctctaatc atttgtattt ctggttccga
     8221 gaaagccgat ggacccctat aagtttcgga tgaagggaca gggctcgaac ccagatcatc
     8281 atagccccac atatacccat aattgcgatt caaatctaca ccataactgt tatccgggtt
     8341 taggcgccgg tttttcctcc acattcctcc accatttgga ctcaccagat ggttgaattc
     8401 ataaccatcg ggatttacac agggaatgaa aaaaatctcc gtcgtatcga tgagctggtg
     8461 aatataaggg ttggaatcat aattttctaa aagataatac ataaaaaata acatctgctg
     8521 cataccaatg ggttcgcgtg catgatgaag agccgtaaat aaaatcctgt tctcgctttc
     8581 atccattttg ggatggtcgg aaatctttac ccagaaaagt tgacgtcctt gttgagtagt
     8641 aaggctgccc aaactttgtc tagcattaat caaattggga tatctggcat gcatgctatc
     8701 gaggtgcata taaatttccg aaagcgtgca gtagccaccc attgaaccca gagagaatcc
     8761 tgttggaact ggaatatcat ggatattttg aatgttttta attcgttttt catttctttc
     8821 tatataaaat tccgtaacat tctcaatcac tacttcaaaa ggaattccga tttgagttag
     8881 ttgtttcagc tcattttccg agagttctat catgagttta ttatcctcgg taaaaacgcc
     8941 atcctcaaca gcaatgcctt gtttggccaa ttctgtcaga taggtttttt cgatttctac
     9001 tctcgaatat tttatttgag cttgtaaaaa aatggtgaat aaaagaagac aacaggttat
     9061 aacccatttt ttcggcaatt ttacttcaaa gtggagatgt ggccaatgtt tttccatttc
     9121 agtgtttgag ctaaatgttg aatatcggaa atcaggaaat tggtaaccgg tgctaaggaa
     9181 tcattgttgg gattgaaatc ataatacaaa gctcctctca agaaatgctt tgaactatcg
     9241 gtgagataga actggcaggg cgaagctgct tctcgaccct tgatgagcca gaaaagccca
     9301 taaacgttag cgtcgggatt ctgcaccacg aattcttcaa tggaagaggc tttgggaatg
     9361 tgtttcatca cgaaagtacg agagtcttcg gtgtattgtt gcaaattatc gtgtataggt
     9421 ttataactga ggtaaaccgt tgccttgaat atagggaaga cgatgtcgat ccagtaaggg
     9481 ttttcgacgg gttttcgtgc tggttttatt aaactatatg cagggtattg aaaagcatag
     9541 ggaaaggtag tatcaaaatc cttgtaagct ttttgcggaa ggtcgatgcg caagaaacct
     9601 tttggcttcg gaataggttg atgatggcat gaagtcaaaa tcactgccat ggtcaatacc
     9661 tcaaacataa aaagagagaa acgtttaaac attatttttt tttatcctcc tcttcgtcgt
     9721 aatgataagt aaccctgacc cctttaatgc gtttgtaatc ggccgattct attctaaact
     9781 cgaagttctt gtagaaaata acctcattgg ttgcagggag tctgccttcg agttcaagga
     9841 tcaatccagc aagagtatcg aattctccgc tcacttcatc gaaaatgtct tcacgaattt
     9901 gaagaatacg gcaaaaatca ttcaataaga tttttccatc aaagatgtat gtattttcat
     9961 ctactttcac atagtttttt tcatcttctt cctcatcgaa ttcatcgctt atatctccga
    10021 aaatttcctc caggatgtct tcgagggtca caatacccga agttccacca tattcatcta
    10081 caacgatagc aatatgaatt ttctgacttt gaaattcagt aagtagagta tctatttttt
    10141 tattttcggg aatataatat gcagggcgga gatgtcgctg ccagggataa tctttccctt
    10201 cattgagatg tttcaacagg tctttgatat acagaattcc aactactgaa tcgggtgaac
    10261 cactgtacac aggaattcgc gaatatcccg attgtcggat tatttgtaac aattcatcat
    10321 aaggagtgtt atcatttacg gctacagcat ccacacgggg aaccatgatt tctctgacct
    10381 gaatgtttcc gaaatttacg atgcctttga acattctggc ttcgtcgtgt ttatctgatg
    10441 aggttttcgt aatgttaatg gcctccgaaa tatctttgag tgaaatgtca tcttctcgtt
    10501 tcttcaatct tcgatccact atgcccgtag attttaccaa aacaaagtta attggtttga
    10561 atagcttttc taacacaaac aatggagtag ccataaggcg gataatggat tctttcttgt
    10621 gcgtactata tatctttggg ctgatttctc cgaaaaacag tattatcaaa gtgactacta
    10681 ccgactgtat aataaaggta gccagtggat aatcttcgac gctcactata agattcgtaa
    10741 cataaactga aatgagtaca attccgacgt tgatgaagtt attagcgata aggatagtgg
    10801 ccagtagaaa atcgggattc tcgagcaggt atgaagctct gcgaaaatag ttttcactcg
    10861 aattttttat cttttcgatc tgggatgcac tcaacccgaa gaaagctgct tcactcgcgg
    10921 aaataagacc tgaacatatt aataaaatga tgatagccaa taaacccata ctgacattca
    10981 agggcgcatt ggctacctca actctgcaaa aaattgcatt cccacaaaca aaagatgaaa
    11041 taatagaggg gagttccaag catgaatcaa tttattcccg tgtcaaataa ttgcttacgg
    11101 gctgttaact tttgcaaagt tataaaaaat agaacccttt cttatcattt ccattctcga
    11161 tttagggagt aacgttcccc aaacaagaca tgctgaaatg aattggaaaa tttttctaat
    11221 aagagaggaa attcattaac aatgatcgag cagtagagat cgggttttaa aatggtaagt
    11281 cgtcggtacc gccttcgata ggtggtatct cgatgtcagg ttcggcagct tcagtaacct
    11341 gaggtatacc ttccttcttg ggacttagga tggtaaaatt atcaccttga atcgtgtaga
    11401 agtatttttt ttgtccttgg tcttcatatt cccggcgtct gatttttcct tccagataaa
    11461 tcatatcgcc tttgtggagg tacttttcag caatttcggc cagacctctc catagcacta
    11521 tattgtgcca ttcggtttga tcgactcgtt gaccatcgct ttttttgtag gattcggttg
    11581 ttgccaacgt aaaagtggct tgtttcccat tgttgaattc gcggatttca ggatcacgtc
    11641 ctaagtgtcc taccaaaata accttgttga taccgtttgc catagtgttt aaattttaag
    11701 atttgcaggt aaattaccat tgccaaaagt aagaaaagtt ttcattggtg caaccttttt
    11761 caaaaaaaat ttctaatatt gatttttcat aattttgccg cttattttca acattataat
    11821 aatgttaagg acacatactt gcggagaatt gaatacaaat catataggcg aggaagttat
    11881 tttatgtggc tgggtgcagc gtactcgcga ttttggtggc atgacttttc ttgatttgcg
    11941 cgatcgttat ggaattacgc aattggtttt caatatggaa tgggatgtac acctgtgcga
    12001 ggcagcacgc aagcttagtc gcgaatatgt aatcagagct aaaggaaaag taaccaaacg
    12061 tgaaagcatt aatccgaaat tgcctacggg tgaaatcgaa attattgtcg atgaattcga
    12121 aatactgaac gagtcgaaaa ttccaccctt taccatagag gatcaaaccg atgggggcga
    12181 agaattgaga atgaaatatc gttatctcga tctgcgccgc aatcccttaa ggaaaaatct
    12241 ggaacagcgt catgccttgg ccatggaaac ccgcaaatat ttatgtgaac agcatttcct
    12301 cgaaatagag actccctatc ttattaaatc aacacccgaa ggcgcacgtg attttgtggt
    12361 gccatcccgc atgaatccag gccagtttta tgccctgcct caatcacctc aaaccttgaa
    12421 acaaatatta atggtagctg gctacgatcg ctattttcaa atcgtacgct gctttagaga
    12481 tgaagacttg agagccgacc gtcaacctga gtttacacag atagactgcg aaatggcttt
    12541 tgtggatcag gaagatattt tacaagtctt tgaaggtctt attattagat tgttttcaaa
    12601 agtaaaggga atagaattgg attcttttcc cagaatttca tacgacgaag ccatgaagcg
    12661 ctttggttcc gataaacccg atattcgctt cggaatggag ttcaccgaaa ttacttctcg
    12721 ggcaaaagga aaaggattca aggtttttga tgaagccgaa ataatcgtag gaatcaatgc
    12781 caagaaatgt gctcaatata gccgaaagga actcgaccag ctgactgagt gggtaaaaag
    12841 gccacaaatt ggcgcctcgg ggcttattta tattaaattt tcggaagatg gtagccttcg
    12901 gtcttcgata gataaatttt ttcgacaaga ccacctgaga gaattatcgg cagagttctc
    12961 ggcaaaacct ggcgacttaa tgttgataat agcaggtgca aaagaaaaaa cgctgaaggc
    13021 tttgggtgaa ctcaggctcg aaatgggcaa ccgtcttgga ttgcgaaatc ccaatgtata
    13081 tcgccctctc tgggttatcg attttccact cctggaatgg gatgaagaat tacaacggtt
    13141 ttttgccatg catcatccct ttacttctcc taaacccgaa gatattcctt tacttgagac
    13201 aaatccttct ttagtgagag ccaatgctta tgatctggtt atcaatggag tagagattgg
    13261 aggaggtagc atacgtattc atctcaagga gctgcaaaat agaatgcttc ggttgcttgg
    13321 ttttacccag gaagaagccc agaaacagtt tggatttctg atggatgcct tcgaatatgg
    13381 agctccacct catggaggta tcgcttttgg gttcgaccgt ctctgcgcta tctttggagg
    13441 ttccgattcc atcagggatt ttattgcttt tcctaaaaat aattccggcc gtgatatgat
    13501 gctggattcc ccttccgaga tttcacaaca gcaactcaat gaactttatc taaggattaa
    13561 tctaccataa aaaccttacg gggattcaaa aacctgttag ggttaaaatt tgaagctgtg
    13621 tcaaattcga acctttcctt cagaattgta ggatgccaat gatttctctt acttcgcgaa
    13681 tggttttttg cgcgctggca cgtgcctttg ctgctccttc ggttactacc ttgtgcagat
    13741 agtcattatc ggcatcgagt tgcagaattt tttcacggaa aggcgtcgta aactggataa
    13801 tatcttcggc gagttgtttt tttagatcac catagcgtat cctgcaagta ttgtattgtt
    13861 cttcgaaata ggtaacggta tcgggtgaag aaaccacttt tagcagggta aacaggtttt
    13921 ggacgggttc tgattttttt tgatgagggc gggtaggtcc gtcatcggta acggcgcgca
    13981 taactttttt acggattact tcgggttcat cgctcaggaa aatggcgttg ccttcgcctt
    14041 ccgattttcc catttttccg cttccatcca gtccgggaat tcttattaaa tctttctccg
    14101 agctgtaggc ttgaggttcg gtgaaataat cgacatgata aaggcggtta aaacgattgg
    14161 cgaaagtgcg ggtcatttcg agatgttgtt cctgatcttt tcccacagga accttatgag
    14221 ccttatgaat aatgatatcg gctgccatca gcacagggta agtaagtaaa ccggcattaa
    14281 tgttgtcggg ttgtttacgt gctttttctt tgaaggaagt tacccgttga agttcaccaa
    14341 gataggcgtt catgttgagc agaagatata actcggctac ttcgtgaagg tcgctttgaa
    14401 tatagatagt tgatttttca gggtcgaggc ctgaagccag ataattgatt aatacgcgcc
    14461 tcacattata atgcagattg gaaggcgttg gatgcgtagt aagtgaatga taatcggcga
    14521 taaagaagaa acaccgatta tcttcctgca tttttacgaa gtttttgatt gcaccaaagt
    14581 aattaccgag gtgcaattca ccggttggac gaataccact aacgactgtt tccatgccgc
    14641 gaagatacaa aaaagcccgg aaacagttcc gggcaagatg aacttatgca aatggcgaat
    14701 aatgccttaa attctgattt cgtcgccttt attattgaaa aaatgatatt ctatcatgtg
    14761 aaatgattta ttggcagata tagaaatcca tttcttaaat ttaaaaaccc agcgcagttg
    14821 aatagaaggg aacccttttg taagatacgc atatacataa ggatgaacac tcatttttat
    14881 ttgtttttca ttctgttcgt gaataagata acgtattttg ttttctattt catctacgag
    14941 gatgatgctg ggttttacct gtccggttcc catacaggcg gggcagagtt cgagtacttc
    15001 gacattggtt tcgggtctga cgcgctgcct ggtaatttgt atgagtccga atttgcttgg
    15061 agggagtatg gtatgttttg ccctgtcttt ggccatttct tctttgagat gttcatataa
    15121 ttttttacgg ttttgtggat tgaccatatc aatgaaatca accacaataa tacctcccag
    15181 atcgcgaagg cggagttggc gggcaacttc cgttgctgct tccaggttta cttccaaggc
    15241 attggtttcc tgatcgttat ttttttttgc tcgatgaccg ctatttacat cgatcacatg
    15301 aaaggcttcg gtgcgttcga taatcagata aataccgcta ctaatgctta cggtgcggcc
    15361 gaaagagttt tttatctgct tatctattcc gaaatattcg aatataggta atttaccctt
    15421 gtataacttg acgatattca ccttatcggg agctattgtc tgtatatagc tacgggtttc
    15481 ttcataaagg ccagggtcgt tgatgtaaat attattgaag gattcgttga gcagatcgcg
    15541 caataaggca gatgtacggt caatctcact gatgattttt tgtggaggtt tggcttcgga
    15601 gagttttttc atcaccattt cccatttcgc catcagggac tttaagtctg catcgaggtc
    15661 ggccaccaac tttccttcgg caacggtacg aatgattatg ccgaaatttt tggggcgaat
    15721 gctggtaacc aaacgtttaa gccggttgcg ttcttcctgg cttttaattt tttgtgaaac
    15781 agaaatacga ttggaaaatg gaaccaatac cagatacctt ccggcaaatg aaatttcgga
    15841 agtaacccga ggcccttttg tagatatggg ctctttcgca atttgaacga tgacaggttg
    15901 attaggagca agaacttctg aaatctttcc tgttttttct atatcggctt ctggctggaa
    15961 atcagataaa tcagcatgaa aatgcttccc acccataatc tggcgggtaa gtttattgag
    16021 cgattggact tgaggtccaa ggtcgagata atgaagaaaa gcatcctttt cataaccgat
    16081 attaatgaag gcagcattaa ggccggtcat gagttttttt acttttccca agtaaatatc
    16141 gccaacagca taattattgt tcatcttctc cttatgaagt tccacaaggc ttttctcttc
    16201 caaaagcgca atggatactt cattggagtt ggcatcaata attaattctc tggtcacgct
    16261 gttaggaaat taaagtttac aggaaaatct cctgaaaaga aaacttgtat tacatcatcc
    16321 tttcaggtaa tcaattttaa aaaagtatta tacaacaggg ctcacccgca ttgtataata
    16381 ctttgatgtg aaggtaaaat caggctatta ctattttttc ttgtgccgat tctttcttaa
    16441 gcgtttctta cgcttatgcg tcgccatttt gtgccttttc ctttttttac cgcttggcat
    16501 tttttatgga tttgagagtt attttacgtt gtttttcact tcgttcacaa aagacttggc
    16561 tggcttaaac gatggaatat tgtgagccgg aataatgatg gtggtattct tcgaaatatt
    16621 acggcccgtc ttctcagccc ttcttttgat tacaaagctt ccaaatcctc tcaaatagac
    16681 attttcgcca tttatcatgg ctttcttaat ggaatccatg aaagcttcca caatttgctg
    16741 aacagcaatt ttttcaacat tggttttgtt ggcaatttcg gcaacaattt ctgcttttgt
    16801 catatctaat agttttaaag gtttgattat taaattccta atgggctgca aagatagtaa
    16861 catttttatt ttcacaaagt tcttcgaaga ttttataaaa agtttatgag ttacctttgt
    16921 cggagtgaat gaataaccct actccctttg ccgaatctct gttaagctgg tatgctaaca
    16981 atgcccggga tttgccttgg agaatcgatg tttccgctta tacaacatgg atttcggaaa
    17041 tccttctgca gcaaacccgc ttaaatcagg gaataatcta ttatcatcgg tttattgagc
    17101 gtttcccgga tataagtagc ctggctgaag catcggaaga ggaagtatta aaactatggc
    17161 aaggcttggg ttattgttcc cgggcaagaa atttacacga gggagtccgc cagattatcg
    17221 aaaattatca cggacaaatt ccatcttcct ttcaggaatt aaaaaaaata aaaggcattg
    17281 gatcttacac ggctgccgct atcagttcta tcgtttttca ccaagctgtg ccggctatcg
    17341 acggaaatgc catcagagtt tttagccact ttatgggaat aaacgaccct gtggacactt
    17401 cttcgggccg tatttcgata gaaaaagctg cttattcgct gatggatcaa cagcgtccgg
    17461 gtgatttcaa tcaagcggtg atggaattgg gagccttggt ttgtacaccc aaacgtcctg
    17521 cctgtttttc ctgccctttg ataaatggtt gtatagctta tcgtgaagac aaaatcgatc
    17581 tgattccttc aaaacataga aaaacacggg tcaaagagag atacattttt tatctggtca
    17641 tattaagcca tgccaagaat aatatgcaag tgattataaa aaaacgagat cagaacgata
    17701 tctggaaagg tttgtatgat ttcccttcct tggaattcga tggtccggct gataagacag
    17761 cagtaataga atctgagaat ttgaaaatcg attttcctta cctttctccc gctgaattaa
    17821 agtttacaaa aggcccatcg aaacatatcc tcagccatcg taccttatgt cttaatttcc
    17881 tggtctttga atcgaccatg ttgcctgcta ttaaaaacaa gaaacataga gtgatttcaa
    17941 tagatgaagc tattcatttg cctttgcctc gtccgataga aaaattcctg gaagaattaa
    18001 ttgttgggca aataccatga aaacatctat agggaagaac gagaataaaa aaattcgatg
    18061 aacgggatga taattttctt atttccctca caagtgcctg ttgcaaaatg gaatgtgaaa
    18121 tctgtgccct ttaaaaattt tggagagata aggtattatt tcatgaaggt ttcaggattc
    18181 tgattctttg aatttcaatc ggcagctcag ggaatggaaa ggcgggtgag gttacatagt
    18241 ttttgaagaa tgcgagctcc aacgttggcg tcccattcgt catcggaacc aggagcaacc
    18301 tctaccagat cgaaaccaat gatttgccgg cccgaagttg ccagaatatg cagcaatagt
    18361 acaacctgat cgaatgaaag tccacccgga acgggtgttc ctgtgtgggg gcagagttcg
    18421 ggatttaatc catctatatc gaaactgaca tacactttcg aaggcagatg cttcacgatt
    18481 tcttcgcaga tttcgctgaa gggctttcca ttcaaagttt cgaaggctag taaatatcca
    18541 tcgtattgct tgattctttt gtcgtgatat cccagcatca tttcatcttc acaaacatcg
    18601 cgtatcccca cttgaacgag tttctcaagc gatttcaact ccagtgcatt gtacattgat
    18661 gaagcatggc taaagataaa accttcgtaa gcctttctta aatcggcatg agcgtcgatg
    18721 tgaagtattc caaaaccatc gtattgcttc gtcaacgcac gcatcaatcc taatgaagtg
    18781 ctatgatcac ctccgagcac acaggctgtt ttacccttcg aaatccagta ataactggtt
    18841 ttataaaccc attggttcat cttttcacat ccaagattaa ttttttcgag taagggcaat
    18901 atctgggggt tatttgcatt catgccagtt tctaaaagat ggattacctg ctgggcaagc
    18961 ggccgcaatt gttcattgag ctgcaaaata tcttcagata gaggcagagt ggctattcga
    19021 ttcttccatg cctgttcgag aaagaaatca aataaatcga cctgagttga ggcctttatg
    19081 attgcctcag gcccctggca agtaccagga tgataagaag ttgtaacatc ccatggaaca
    19141 ggaataatga tgatatcggc ttcttcgttg ctataaggca aacctaagaa atttccattg
    19201 ggaatgccaa tggcattggg atcgaatgaa gtcatataat tggtattact tatgcaaaat
    19261 tagtgatttc gatataagcc tatattatac ctcttctcat ctgaatattc gaaaattcgt
    19321 gttatttata aattcaaaaa tggatcgtag tttattgact attagcttgt agctttatag
    19381 aaatgatggc aactggcatt ctctcttgct aatttagtcc accgattcac actgatcgtc
    19441 tgcggttatc tgcaagaact ctttattttc ttactgattg acgcagatta aatccgctga
    19501 ttataacaaa caacaaacaa ctacaagatt tcaaattaca agcaaatttc aacgaacaac
    19561 tgacaaggaa caacttacaa ctacaacatt tcaaattaca aattccaaac tccaaatttc
    19621 agtctcccag tctcccagtc tcccagtctc ccagtctccc agtctcttcg tctccttgtc
    19681 tccttgtctc cttgtctcct catctcccag tccccccgtc cccctcgtct cccttcctcc
    19741 cttataaact tttaactttc tactccccat ttcccatttt cgatgaaaag cgaattttta
    19801 tcaattttgt gtgtcgattg aaaattatca tataaattta ctggcccaat acaaggtatt
    19861 attcatgcca gcacaaatca tttcattgct cgatgatttt ttatcgctta tttatccacg
    19921 cttatgctat gcgtgtggga atagccttta cctgaacgaa gcggtaattt gcaatcattg
    19981 tttagcaacg ttgccactta cttatagtca tttgagcaaa gacaatccag tagcccggac
    20041 attttggggg aggattccgg ttgaagctgc cgcagcaaga tattatttca ggaagggcgg
    20101 gcctgttcaa cgattattgc ataatctcaa atacaatcac gcacccgaga tcggaaaatt
    20161 cattggattg atatacggaa gggaattatt agaatcggag gtattccgtt cggtggattt
    20221 aattatacca gttccgttgc attttgagaa attgaagaaa agaggataca accaatcggc
    20281 catgtttggg caagggttga gctcgagcat gaaaattcct atagatgatg aaagtctatt
    20341 tcgtacttat tttacagaga cccaaacccg gaaatcgcgc gagaaacgat gggaaaatgt
    20401 gaagtcggtt tttgctctta aagaaaatgt ttctttaaga ggcaaacata ttttgattgt
    20461 agatgatgta ataaccacag gatcaaccat cgaagcctgt gccaatagat tattggagat
    20521 ggagggagta aggatcagcg ttgccgcaat tgccttcccc agccatattt tttgagaaga
    20581 tgtgacgttt ggtttttcac tttttagtct cctacccgca ctctccggcc gccatccacc
    20641 atttagcagc ttactttgca tagccctttc ggttgtcaga gtagaaataa ttattgtacg
    20701 aaaatctatg cccctgttga ggaggttcta aagccgagtc agtgcccaga agcggccata
    20761 actccgagtt ccttatcaat gaggtataag cccctgttct gaccatcgcc ggcgagtttc
    20821 atcttgtcga gaatagtccg cactaaagtt tcctcttcga tctgctcatt gatgaaccaa
    20881 tgaatgaaac ttgcagttgt gtaatctttt tcttgtaggg ttagaccata aatttggtta
    20941 atagaagcgg aaataaattt ttcgtgttcg agaattttct cgaacatttc tttaagtgag
    21001 gggtaatcgc tttgaggggt ctcgaggggt ttcaatagag ccataccttc tctatcgttc
    21061 agaaaatgga tgaatttcag ctgatgaaga cgttcttcgt cggaatgttt gtataaaaag
    21121 gctgccgctc cagggaagcc atttatttca caccatatag ccatttgaag ataaagcctc
    21181 gaactgtgct cttctttctg tatctgttcg atgatagctt gctcgatatt ctttttcatg
    21241 tttttgtatt ttatcgaaat aataaccatt taaacctaaa ctttgttcaa tgcgttcgaa
    21301 cctgaaacga tttcgaggat ttcttttgta atggcagcct gtcgggcttt attatatgac
    21361 agggtaagat ccttcaaaat cacatcagca ttatcggtag ctatcatcat ggcagtcatt
    21421 cttgcacctt gttcggaagc aaaggaatcg agaaaagttt tataaagctg gatatgcaag
    21481 taatgaggga taagttcttc gatgatggtt tccgatgaag gttcaaagat ataatcgatg
    21541 tttcgggttt tcttattttc atcataagaa agagggagaa aagtttcggt agttacgact
    21601 tgtgtggcag aatttataaa ctggttataa accaacacga tacgatcgaa ttcgtgtttc
    21661 gtccacaatt cgatcaattc gtcggccagt aattttactc cttcccaggt tactttttct
    21721 ataaggttat ctttgatttg caacacaggg tattttttgt tgatgaggat atcgcttacg
    21781 tgttttccaa tactgatgag ttttagatgg tttaagccat attgttgttc aaaagaagtt
    21841 tcggtgagat attttatctg tttgatgata ttggagttga aggcgccaca caggcctctg
    21901 ttggaggcaa ggctcaccag aagaacttta tcggtttcgt gctgttcgaa gaaaggggaa
    21961 gaaaggtttt cactataatt tgaatagaga gtcgaaatga gttcactcat tttctgagca
    22021 taaggtcgga gggaaaggat ggcattctgt gctttgcgca gtttcgaagc cgctaccatt
    22081 ttcatggcat tggtgatctg ttgagtattt ttaaccgatt cgattcttaa cctgacttcc
    22141 ttgagattcg acatgggcta atatttttta ttcgtatcgt ttgaccagtt tggcaactat
    22201 ttcttccaat ttattggcaa cttcatccgg aaactggccg gtttgaatag tttggagaat
    22261 gtcgggatat ttttcgctaa gcagattcag atattctgtt tcgaaatcat gtatgcgatt
    22321 tactggaacg tttttcaaga ggttgtgcgt accgcagtag atgatagcaa tttgttcttc
    22381 aacgggcagg ggttgatgaa gaccttgttt gaggatttcg acatttcgtt ttcctttttc
    22441 cagtatggca atggttgctg gatcgaggtc agaaccaaat ttggagaaag cctctagttc
    22501 gcggtattgt gcctggtcga ttttcaaggt gcctgcaatt tttttcatgc ctttgatctg
    22561 tgcatttcca cctactctcg aaacggagat tccaacgtta attgctggtc ggattcctga
    22621 attgaaaaga ttggtttcga ggaaaatctg tccatcggta atggaaatca cattggtagg
    22681 gatataagct gctacgtcac cggcttgagt ttcgattata ggaagggctg tcagtgagcc
    22741 tcctcctttt actttatcgc ggatagattc gggaaggtcg ttcatttggg ctgcaatttg
    22801 gtcgctatca atgatttttg ctgcacgttc gagcaaacga gaatgcaggt agaagatatc
    22861 tcctggatag gcttcacgcc ctggaggtct gcgcaataaa agtgaaactt cacgataggc
    22921 tactgcctgt ttggagaggt catcataaat aataagagct ggacgtcctg tgtctctaaa
    22981 atattctcct atggccgctc ctgtgaaagg agcataaaat tgcatggctg ccggatcact
    23041 ggcagtagcc attactacaa tcgtataagg cattgctccg aaatcttcga gtgttttcac
    23101 aaaattggcc actgacgagc ctttttgccc cactgctaca taaatacagt aaaccggttc
    23161 gcctttctca taaaattctt tttgattgat gatagtgtca agggcaatag atgattttcc
    23221 tgtctggcgg tcgccaataa taagttctct ttgccctctc ccaataggga tcattgcatc
    23281 gattgcttta atcccggttt gaaggggttc atttacaggc cgacggtaaa ttactccggg
    23341 agcttttctt tcgagaggca ttctatagag aggccctggt atatctcctt tcccatcgat
    23401 ggattcgcca agtgtgttga tcacccgtcc cagcatgcct tctcctactt ctaccgaagt
    23461 aacttctcca gtccgtttta caatgtcgcc ttcctttacc aagttcgatt cccccaatag
    23521 cacaacccct acattatctt cttcgagatt aagaacaatg gcttttactc cagtgttctc
    23581 gaattctacc agttcgcctg attgtgcatt cttcaatcca taaatacggg ctatgccatc
    23641 gcctacctgc aatacacttc ccacttcgtt gagttcggca gtggtcctgt ggccagctaa
    23701 ctgctggcgt aaaatcgcag aaacttccgc cggttttatt tctaccattt ctgtaataat
    23761 ttttaaaact gagttacaaa taaattctcg ctaaattctt ttttaagctc ttttaactgt
    23821 tttaaaagag aagcgtcata aagataatca tcgaaaaata actgaaatcc tcctattaat
    23881 cgagggtcaa cctccacttc gagttcaatg gtgcctttta tgacaggttt caatttctcg
    23941 atgattcggg ctttgttttc ttcgttcata ggcgctgcca cctttattct tacttcatag
    24001 atatgatgaa tttgacgata aatttcgata aaagataaaa cgatttcatg aagtatgatt
    24061 tcacgggctt tatcggttac aagcgtaaga aaagtcagtg aaatgtcgtt gatcttgtcc
    24121 ttgaaaagtg caattaaaat atttcttttt tttgaaggag gaataatagg gctaatccag
    24181 actcttttca actctggatt ttcgatgttc accctatcga ctaattgcat gtcttcgaac
    24241 acctcttcca acaggccaga agaatcggcg atttcgtaca gggcgagggc atacctttga
    24301 gcaaggactt tatgacgaat aatcttcatg aattcagtta ggttcagctt aaggtaattt
    24361 cattgatcaa tctttgcaga tattcgtttt gtttttcttc accactgagt ttttctctca
    24421 aaagtttttc ggctacttct atagagaggg ctccgatctg gtttttcaga tttattaagg
    24481 ctgcattttc ttcatttttg atggcttgac gagctaatgc taaaatatga tcatattctg
    24541 tatgggctcg ctgccgagct tcttctaaga tgatatcggc aagtttgcgt gcatccgcca
    24601 ataaagcatc gcgttcctgc tttgcttcgt tgagcagtct ttcgttagtg gccatgagct
    24661 ggttcatctc ttgacgggct ttttctgcca actgcaaagc ctcctcaatg tttttttcac
    24721 gatttctcaa ggccttgaga atggttggcc atgcaaactt ccccagtata aaaaccagta
    24781 ttccaaatga caatgtcatc cagaaaatca aacccacttc aggagtaatg agttccataa
    24841 gttaatatta taatttaacc ttgagtaaaa cttataatcc cttaacgacc ctcgaataag
    24901 attatttctg aagggtacaa aaagggttaa gattcaaact tttacacgaa caatatcaac
    24961 atacaaacca ctacggcaaa gaaggcaact ccttctatta atgcagcggc tattatcata
    25021 ctagagcgaa tgtcgttggc agcctcaggt tgacgagcga tggcttccag ggctcgagct
    25081 cctatgttgc caataccaat ggcggctcct atggctgcta tgctggcacc taatcccgca
    25141 cctatctttg caaaaggact taaatccctt gctgcctgta atatcaatga aagtaatgtc
    25201 ataattgtat tgttttaaga agatgaatgt tatttatgat tagaattttc aatttcttct
    25261 gttgccatac caaagtataa tgctgataat aaagtaaaca cgaaagcttg taaaaaagaa
    25321 accaggattt cgaggaggct gataaatatt gacattagaa ttgatatcag gcttattccg
    25381 taaccgaagg cgggatttat gttgccaaag acgaagatca gactcatgaa acccagaaca
    25441 atgatatgtc ctgccgtcat attggcaaac aaacgaacca tcaatacaaa aggttttgtg
    25501 aataagccaa ggaattcgat cagaggcaaa ataggaattg gtattttcag ccaccaggga
    25561 atgccgggag tgttgaaaat atcttgccaa taatggcgat tgccactcaa gttggtaatg
    25621 agaaaggtaa ataaggccag catcatcgtt accgtaatat ttcctgttac attggcaccg
    25681 ccgggaaaga atggaacaat gcccaaaagg ttattgaaaa aaatgaagaa aaatacggta
    25741 agtagataag gcgtaaatct ttcgtaatgt ttttcaccca gcgatgattt tgcaatatcg
    25801 tcgcgtacaa aaattataat gggttcgagg agtgattgta gccccttagg tgcatgcttt
    25861 tcatttcgtt tgtatgccct cgcaactgtc aaaaaaatga ttatcaatgc tatgacactt
    25921 aaaaataatg aagccacatt cttggtaatg gaaaaatcga gtggaatagt tggactttta
    25981 ttttcattac ctaggctatt gttccccaca taaatgattt tattcttgta agccccgtcg
    26041 gtcatatatc tgaaacctcc gtaatcggat tggccatggt gaaatttcga gtaacaaaaa
    26101 atgtgcaatt tgccttcatg ccataatata attggcaaag ggatgcttat atgggtattc
    26161 cctatactta aaatatgcca ttcgtgcgca tcggtgatat gctcgataat catttcaccc
    26221 ggtttgaact cagcggcttt tacgggtgaa ttctctgctt ttaccaatgt aaagggaaag
    26281 aaaatagcaa agataaaaaa gaagagcttt ttaatcattt ctttgataat caaataaata
    26341 tcgagttttc ttcctcataa gaatgtaggt gcaaggataa tacttttttt attatcatgc
    26401 tatcatagcc aatggaatat gcatgaattc agcttttcag tagatttaac cattgaattt
    26461 caatttcaag gaatctgtta tttcactctg gtaaccacca ccatattcga cgtggctctt
    26521 tctttcatgg ggaatccggt aatcttcacg aggatatcgt cggggttaac ggcattgttt
    26581 tgaagcaata tctgcaccat gaggtcgagg ttgtcgtcgg tataatggag gttttgctgt
    26641 acgaaacccg taatgcccca aacgagcgaa aggcggcgga tgagtcgggg gcttcgggcg
    26701 aagataaata tttgtgcttt cgggcggtag cttgcgagtc gcaaggcagt ataacccgta
    26761 agtgtatagg tgagaatggc ctttgcttca gttttttcac tcaaatgtat agcagcctcg
    26821 attacagcgt cggaaatcaa gcgggatgga tttgtaaatg agttttcggg tttgcggttg
    26881 tggatttctc caattttttc tgtttcatcg atgattcgcc gcatagcttc gacggcttcg
    26941 acgggatatt gtcccatcga agtttcggca ctgagcataa gagcatcggc tccatccatt
    27001 accgaattgg ccacatcatt gatttcggcg cgggtaggcc ttgtgttgtt gatcatcccc
    27061 tccagcattt gtgtagccac aatcacaggc ttcgattgtg ctatggattt gtggatgacc
    27121 aatttttgta ctgcaggaac ctgttcgtaa ggaagttcta cgcctaaatc accccgagca
    27181 accattaaag catcgctaac ttctatgatg gaatcgaaat tttttaaggc ctcgggtttc
    27241 tctatcttgg caatgattcc tggattccag ttgcgaccta aaaactgaat attttctctc
    27301 aattttaaaa tatcttccga acttctcaca aacgacaagg caatccaatc gagttgattt
    27361 tgataggcaa atcgcaaatc tgtcaaatct ttttcgctca gtgccgaaag agttaaatgg
    27421 ctgtcgggta aattcactcc tttacgagct gttagaatgc cacccactaa tacctctgcc
    27481 cgtaaaaacc ccgaaggaag tttttctttt actatcagac taagcttgcc atcgtcgagt
    27541 aatacctgat cgccaggttt aacgtcttca gtcagtgctg gatagtttat ccaaagaata
    27601 tcttctttag acaaacccga gccgcctcca agctgaattt cttgaccttg ccgaaggttg
    27661 atggcattat tttctacttc tcctatacgg atctttggac cttgtaaatc gcctaaaatg
    27721 gccaaacgat gatgataagc aggtcctaat tctcgtatat aatgaattat ctggcgaagt
    27781 atgttatgat cgccatgcga gaaattcaac ctgaaaacat ttacacccgc tttggtcatc
    27841 ctttcgatta ccgacttgtc gaaagacgca ggacctaagg tagcgactat ctttgtgcgc
    27901 atatctttat ggtatataga gtaaaacaaa actaaatgag gccgaatacg agtattttaa
    27961 ggtttatgcc gtatcaacga tgagaatttt tcatttttat gtaagggggt caaagttcta
    28021 aaaaaacagt catttctgca ccatttatat tttgaacata atcttgaatc ggttactttt
    28081 aatgtgctta atctgcataa ttcgagcgat atagattaac cgaaaaatac attgtattga
    28141 aaggcatatt ccaattcaat aaacccttag agaggatact taacactgtt attgcattta
    28201 tttttttaac atctgctcaa caaattcagg aacttcggct tggttgctaa agatatgata
    28261 aagccctgac ataatcggaa aagtattcaa atgcttggaa tgaataatct gaccgcaaaa
    28321 tatattaacg gccattttac cttcgagcag taccttctcg gaaatctgtt cggtaaaata
    28381 acctttcccg ataagcagac ccagtgtacg gtttcgtgaa aggtcgttca atgcggtaag
    28441 cccaaaatcg ccaaaaccgc aatacttgaa catggtagtt ttcttccctc caaacaagat
    28501 atgaatggaa cgcatttcgt tgaaggcacg agtgagaaac aagaagcgca tattggccgt
    28561 attgaactgg gcatccatta tgcccacggc tatggcatag atatttttca agatactcaa
    28621 tagttcgacc cctttgatgt ctttcgaaaa atcgagatat acctgagtat tggcaaagag
    28681 ttcctcaaaa agaggcctca atgtattatc atggcatccc acggtgaaag ctgtaggact
    28741 tccattaatc atttcttttg cgaaagaagg acctttcata gctcctgctc ggaatggaaa
    28801 ctccttgatc aatccttccg aaatggtttg ccctccttga tcgaatccct tagcaagatt
    28861 tattagtaca acatgaggag gcaaaagaga agaattttca cgaatatatg ggattacggc
    28921 aaccgatggc agagctaaaa acaacagatc ggcttctctt agcacagatt tatctgcggt
    28981 ggctttcact tcactggcca acctaaaacc agggaaatat ttcgaatttc tatggaaatg
    29041 gtttatttca tcaattacct cgggttcaat ggaaagaata gttgtatcca actcgtcatt
    29101 gctggcaagt gcttgagcca tagcagttcc aatagaaccc gagcccacta tgactatttt
    29161 cctgaatttc atcgtatttt ctgtattcat tctttgaacg atttcttgat atagagactg
    29221 cgaaaaattt gccgtaaacg atttatctca tctacattaa aaaatttcaa caaatataaa
    29281 atcaaaggga aagcaattaa caacaataat ttaaagagta tgctcgctaa aagattccca
    29341 accggtacaa aataaccaat ggcaaataag cttaatccgg tgatgagtac ctttgccaaa
    29401 cgaatccctt cgaaatggat ataataaaac ttctgcgaaa aaaaatagaa gaaaatagct
    29461 aacaatccat tggaaacaag catgatgagt gctgctccat aaatacccat tttaggaacc
    29521 agtaaaatat tcattccaat gctaaaaatt gcacaaagca ttacaatcca tgcgttaaac
    29581 tttgtttttt ttacataatg caaaccaaaa gagaagtaag tttgtgcacc cttgagtaca
    29641 aatccaaaac taatgagtgg aataactaca tatgctctca tataatctac attgcccagc
    29701 gtaaatatgg ctactatttc atgcgaaaaa atggaaagag caaatgccga aatcatcaat
    29761 attccaaaat aataagtaaa aatacgagag aagaatcttt ttgcttctgc atcttcaagc
    29821 attttgtagg caataggcaa aaaacccaac tgaaacgatt gaatgatgaa catgttgata
    29881 acccctccaa acttgtaacc tattccataa acccctgtat cgcttcgagt gaggaaatgt
    29941 tgtaccacat agcggtcgcc aagagaaagt atgatggccg aaatgcttgt aaatatcaat
    30001 gggaagccat atctcaacat ctcttttaaa ggacctgatt caaactttgg tacaaggtgt
    30061 tttaaaagaa acgggaatga aaaaacattc aaaagaaaac tggctatgag ctgactcaac
    30121 aaaatggctt ctacccccat tccccggcct acaatgaaat aaacattcaa tcctaaattt
    30181 acggtgagtt taagagtatt aagaatgata tactgccaag gtttctcgtg aaatctcaat
    30241 aaactaagaa ccaataaatt aagtacatcg aaggtgacca gtaagaacga aatatccaga
    30301 tataatttaa actgagaatc gccataaact ccacgatgat ttaaccatgc attgaaaggg
    30361 aaagctaata agttgaatgc cagcaatata aacaccaaag ccatcaatgt tgtaaaaacc
    30421 atacttcctt gccgtttagg atccttctca ggagctaacc atcttaataa tgccgtggga
    30481 aggctaaacg acagaacagc cgaaagaagc tgccaaattg gttctatcaa gatgagggaa
    30541 gaataatcgc taatggaaat atgttgcgaa taaagtggca gtaaaacaaa gcctaccagt
    30601 ttggaaaata tatttccaag actatagata aagacctgtt ttaccgataa tctaaatttg
    30661 tcgaacatct aactgttttt ttccagtaaa accattaacc agtaaggtcg tttgggggag
    30721 attatcacgt tgataccgtc gatgagaaat cctaaaggat gaaatttgta gaaataatag
    30781 aatttatgat tgcattgtgg gcatatagcc gtacgattat tttgatctat ccaaaagtaa
    30841 ggaatccatg agatggaagg aatccatctg cgtttgagcc tcaataaggc tggatgataa
    30901 tatctcacgg gttttccaaa agtaaattcg gctattttct tatattcggg gaaaagaacc
    30961 gtgaaatcct cgcttttgaa agatctgagg tgattgggtc ggttgaaaat atatttacat
    31021 tcagggcagc gaatcattaa tttatcgggg ttttcgcggt tgggtacggt aatatatata
    31081 tatttttttg atattctttt gaattcttgt attgtttttt ggaatatttc attgggaaga
    31141 tgttcgagca attcggatga aaagaccata tcgaagttct gtgaaggcag gggaatgttg
    31201 tcgcacgaag cctccaattt ctcagaggtt acatattgca atgccgactt gctacggtca
    31261 actccgagaa cttggtattt ttttgccagt tcgttagtaa tcactccatt gccacagcca
    31321 atatctataa ttgattgtac atcagaaggg atattttcaa tgattttctt gatttttggg
    31381 ccgagctgtt tattagtaag tatctcccaa tctacttttt catatataat aggattatcc
    31441 atcattttgt ttgatcaaat tcagaataca attgaattaa tttttgagca tctttatccc
    31501 aattatattt attaactata aaagttctcc catattcagg attaaatttt cctaatgaaa
    31561 taagattttt taattcaatt aagttttcag cgaaatctgt tggcgaatca tttttatata
    31621 taattcctga atgagtttct tcaataatcc ttcttaaagg attgcagtca gaagaaagaa
    31681 tgggtatgcc cgcataaatg tattgaaaaa ttttgtgagg aatagtagta tctgtatgct
    31741 ctgattttaa atgagggatt acggcaacat ttgcattttg aagaatagca agcatttctc
    31801 caaacggcag gtaaccgtaa aatttaattc tactggaaat atttaacgta accgaaagct
    31861 ttattaattt ttccagatat ttaccatcgc cgacgatgtc aaagttaaaa tcagaaaagt
    31921 gatcatttaa aagggcaatg ccccttatga caatttgtat acctctatgt ttattgattc
    31981 caccaccata aaaaatgtta aaattatttg tatttgacgt atttacttta tattttggca
    32041 atttatcaaa agactcaata tttaaggtat tactaaccac agtaatttta ttgtgttcaa
    32101 ttcctaaatt ttccaatctt ttctgggctt cttccaccac aacaataact ttatcagcca
    32161 aattacacat tcttttttcg taatttctcc attgttgatc atgtgaaaaa attttcccta
    32221 caaaagactg ggtatgttca gaaattttta atagtcctgg ccaattttca tgcaaatcca
    32281 atacaaattt tgcgtttagt ttttttgaaa cttcgaaacc gatttgggct aatggtagat
    32341 catgaacatg aattgcatca tatttgtttt ttaatagcaa tttatttaat tcgtttctcc
    32401 aaaaattgaa ataaaatggg agtgtaaggg tagcaatact tgatttatat ctcaaagcag
    32461 gaattttaaa acgcttgatt ataactttgc ctttgaaaaa ttcatcagaa ggcaattctt
    32521 tacgagtcaa acatgcaatt gaaatttcat ggccagcctt tgcaagtgcc atagcttcgt
    32581 tttcaactct tatatctgga ggaaattctc ccggaagaag catcaatatt ttcattaagg
    32641 ctgcaaatga tgtaaaatta tttccactat tctttttgaa gttttcccat cccataaagg
    32701 tggtatcatt gtattttgca aggaattatt aaatacttct atggctttta atgctgcatc
    32761 gaaattggat cccacaagct gattcgttcc catctcaata gttacaggcc tttcggtaga
    32821 atttcttact gttatacaag gtatttttag aaaacaagtt tcttcttgta ttcctccact
    32881 atcggttacc acaccttttg aatcaataac taaacttaaa aaattaatat atccgagagg
    32941 ttcaattaaa tgaacatttt tattaatttt ctctaagagg tgaaattttt tgaaattgtt
    33001 caaagttcgt ggatgaatcg ggaaaactac atcatattct ttagatactt catttaaaaa
    33061 tttcacaagt tcatttaaat tacctaggct atccacattg gaaggtctat gaaaagtagt
    33121 aacaatatat tctttagggt tgaggtttaa agatgaaaaa aagtgtttat tgctaatttt
    33181 ttctaaattc tgcattaaat tatcgatcat ggtgttccca acaaaaaaaa tcttttcgtc
    33241 agccactcct tcatatttca aatttcttaa tcctgaattt tcggtaacaa ataaataatc
    33301 gcttatggca tcggtaacaa ttctattgat ttcttcaggc atcgatcggt cgaaacttct
    33361 caagccggcc tcaatgtggc cgacttttat tccgagtttc actgctgtta aggaacatgc
    33421 taatgttgaa tttacgtcgc cgggtactaa tactatgtca ggttgatact gttgaagaat
    33481 tttttcaaaa cgcaacataa ttgtagctgt ttgtttagca tgagttcccc ctccagcatt
    33541 caggaaaaag tcaggtgttg gaatatctag ttcatcaaag aatatatccg acatcaatgt
    33601 atcgtaatgc tgaccggtat ggcagatctt atgttcacaa agggattcaa cttctcgaaa
    33661 agccttatga attggagcaa tttttataaa gttaggcctg gcgcctacaa cggaaagtac
    33721 ttttttcatt ttcgaaattt tgagtttaca gagttataaa tttttattct tccctgacaa
    33781 atccaaaaaa aattttccgg acgcttagtt atctagtttt attaaattcg atgcaaagtt
    33841 aagataagat ggtcttatga gactggacaa tatttttatc aaagttaatt tattttgttg
    33901 aaaagagggg gtgtggggga gactcataac ttgatttttt gttaataaat tatgcagcat
    33961 tctgatat
//
LOCUS       DAOORU010000010        32566 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_1577_length_32566_cov_6.569346, whole genome shotgun sequence.
ACCESSION   DAOORU010000010 DAOORU010000000
VERSION     DAOORU010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 32566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32566
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_32566_cov_6.569346"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            431..1675
                     /locus_tag="PLF20_01610"
     CDS             431..1675
                     /locus_tag="PLF20_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79741.1"
                     /translation="MKQEIIDNINNPENLEKLYRNNKQAFIKSFEEISDDYNSDLVRF
                     WKIRLASEKEPAFKGFLKSDLLVVISLSLITGLLVKLPEIFSQIQSEFFYTRDLAIIV
                     FNGLILYTFWQNRLFKGKPLLIYSSIIVLLTLFVNFLPNTQSDSVMLSMIHVPLFLWC
                     LFGLSFVSFDYKNIQRRIEFIRFNGELIIMTGLILIAGGLLTLITIQLFSVIKMNIEN
                     FYLHYIAVFGSVAAPIVSSYLIKLYPNLTNRIAPVIARVFSPLVLITLAVYLVSLIFS
                     KNKILEDRDLLILLNIMLLAVMAIIVFSVSELDKSKEKNINVLILILLAALAIVVNSI
                     ALIAIISRVTMGLTPNRTVVLISNILIFINLILITKNLFESYIKNEPLDSVESTVAKY
                     LNIYFYWTIIVIFVLPFVFGLK"
     gene            complement(2399..3589)
                     /locus_tag="PLF20_01615"
     CDS             complement(2399..3589)
                     /locus_tag="PLF20_01615"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOX79742.1"
                     /translation="MKTIDDFNFKNKRALMRVDFNVPLDDKFQIADTTRIDVTLPTIR
                     KILNDGGCVILMSHLGRPKDGPSDKYSLRHLLPYLRKVTGVEVKFAPDCVGEDALKMA
                     LELKPGEILLLENLRFYKEEEKGDEEFARKLARLGDVYVNDAFGAAHRAHASTAVIAK
                     FFPQAKMFGYVMGNELAALKKVLEEAEHPFTAILGGAKVSGKIDIIRNLMKRVDNLII
                     GGGMIYTFIKAQGGKVGNSLVEDELISLALETLEKAKQLGIKIFLPVDIVIADKFDNE
                     ANRKVCKADEIPDGWMGLDIGPKTIELFKDVITHSKTLLWNGPMGVFEMSNFETGTVA
                     IAQCIAEATAKGAFSLVGGGDSVAAINKYQLADRVSYVSTGGGAMLEYIEGKELPGVK
                     FVDE"
     gene            complement(3796..5949)
                     /locus_tag="PLF20_01620"
     CDS             complement(3796..5949)
                     /locus_tag="PLF20_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOX79743.1"
                     /translation="MKTFHYPFTTILFTFIFMTHLQLDAQQNLRPLKQYLYPAPPIAA
                     SRPVEFREFGNTRIDPYFWLKDRNNPEVIAYLNAENEYCKLVMQETEPLQEEIFNEIK
                     SRIKEEDMSVKIPDNGYYYFTRTLKGKQYPLYYRQKMTPQAEEELIFDVNAMAESQPA
                     FIFSDYEVSPDNNFAAYSYNTTGSFAEFTLKIRDLRSGEDLPEQIENVQSFTWAADSK
                     TLFYTIGNESLRPYKVLRHIAGTLAEDTPVFEEDDELLNVSVSKTLSNDYILIESASF
                     TTSEVRMLESDQPDGNFQIFLPRKKDVMYGVEHHKNLGFYVYYKSPESKNGMVYLTPF
                     GKNSNPKQWKVIFPHDEKVKIENYEVFENHLVIQTRSNGLTGLRIYDLDGSKFHNVAF
                     PEPVYVVSTYTNPEYSQKVFRYSYSSLNRPTTIYQIDMDSHSVQTLKVQEIPSGFDPD
                     KYAVERRFAKASDGTDVPMAILYRKGLELNGSNPALLYGYGAYGYSTDAWFRTSVFSL
                     VDRGFVYAIAQVRGGSEMGEQWYEDGKLMKKKNSFTDFVACAQFLIDKKYSQPKKLGI
                     MGGSAGGLLVAAALNLQPDLFGAVVADVPFVDVINTMLDKTLPLTTQEYEQWGNPNKK
                     KDYNYILSYSPYDNIKPANYPPILATAGLNDSQVRYHEPAKWVAKLRANNTGDNIILL
                     KTNMESGHGGATGRYDALKEVAFEWVFLLRALTAQ"
     gene            complement(6152..6766)
                     /locus_tag="PLF20_01625"
     CDS             complement(6152..6766)
                     /locus_tag="PLF20_01625"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOX79744.1"
                     /translation="MHIAIAGNIGSGKSTLTALLAKHFQWKPHFENVDTNPYLMSFYE
                     DMQRWSFNLQIYFLNSRFRQIVEIRKSGENVVQDRTIYEDAYIFAPNLHDMGLMDTRD
                     FQNYQSLFELMISFIQPPDLLVYLRATVPTLVRQIQKRGRPYEESIRLDYLKGLNERY
                     EDWISQYKAGKLLIIDVDHTDFASNTEDLASVIEKINASLHGLF"
     gene            complement(6806..8344)
                     /locus_tag="PLF20_01630"
     CDS             complement(6806..8344)
                     /locus_tag="PLF20_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005783832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HOX79745.1"
                     /translation="MSLTNTLISRYLRRRIERINYFELHAYEVQNWVLEKLITSAKNT
                     EWGKVYDYQRIHNYEDFRKKVPVQDYESLKSYIERVRQGENNVLWNTPIRWFAKSSGT
                     TNDKSKFIPLSKESLYHCHFKAGKDMLSIYVNHYPQTRLFSGKSLVMGGSQTLHRVNH
                     HQIVEGDLSAILMQNLPFWAAKRSTPELKVALMKNWEEKIAKMAQITIKENVTSIIGV
                     PSWTLVLLKRILEDTNRQHIGELWPRLEVFFHGGVSFRPYKQEFEKLISLQHLHYVET
                     YNASEGFFGIQDDSSEKAMLLMLDYGIFYEFVPLDKIDQPDEYALPLWQTELGKNYAM
                     LISTNSGLWRYMLGDTIIFTRQNPYKFRVTGRTCHFINAFGEEVIAENVERAFEIACK
                     ECNAQLSEYTGAPVYFSQNRNGAHQWLVEFEKTPEDFECFVETFDKALQSVNSDYEAK
                     RFHDMVLSSPIIQQVPRGTFYQWMKNRNKLGGQNKVPRLANERNIIEEILEMLQQPTT
                     KTNH"
     gene            8776..9207
                     /locus_tag="PLF20_01635"
     CDS             8776..9207
                     /locus_tag="PLF20_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOX79746.1"
                     /translation="MITEESLRQFGVLIHEVRNVSSDEALALLSDGAVILDVRPLFES
                     EYRRFDVKDVIYAPAEGYEYWVKNLPFDRCLIVADASGLKSRTIASRLLADGFRMVVN
                     LSGGLVDWERRGAPVIVDKSKQLMGSCLCQLKPFYYKKRRE"
     gene            complement(10075..10473)
                     /locus_tag="PLF20_01640"
     CDS             complement(10075..10473)
                     /locus_tag="PLF20_01640"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOX79747.1"
                     /translation="MDGIVEIFHHTDFNGICKKFASEESCLEYLALKKWEGGYICRNC
                     GHNNYCKGNTPYSRRCTRCKREESAKAHTIFHYCRLPLPEAFRFANIVCHNPKISSYE
                     LARYFNLRQMTCWKLKKKIVECLESGLKNF"
     gene            10690..11199
                     /locus_tag="PLF20_01645"
     CDS             10690..11199
                     /locus_tag="PLF20_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOX79748.1"
                     /translation="MKKIFLFTGIVFISLSFVSCGKNQKTESTESKSKETTSPVQIIK
                     LDNGLFCKTIWDYTNSPDRWKYRGNKPSVIDFYADWCKPCKIASLILEDLAREYAGEV
                     NFYKIDTDREKELATLFGIRTIPSFLFIPKEGEPRMTAGIAPGEEATRKMFNDYIQQY
                     CLGNFSEAQ"
     gene            11249..11728
                     /gene="trxA"
                     /locus_tag="PLF20_01650"
     CDS             11249..11728
                     /gene="trxA"
                     /locus_tag="PLF20_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOX79749.1"
                     /translation="MKKINLKLILTIFVFTFSILDFFGQASTKTISNEGKVIYLTSAE
                     FKEKVFNWEKSPENWVFEGDRPCIVDFYADWCKPCRMVAPIMEELALKYKGKLTIYKV
                     NTDKEKEVSSVFGIRSIPSVLFCPTKGKPQMATGALPKDTYIQVIEEFLLGNKKQTR"
     gene            11787..12140
                     /gene="trxA"
                     /locus_tag="PLF20_01655"
     CDS             11787..12140
                     /gene="trxA"
                     /locus_tag="PLF20_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOX79750.1"
                     /translation="MENLTKQTFLEKVFDYEKNDQWKFEGKLPCIIDFYADWCGPCKV
                     VAPILEELSKEYAGRVNFYKVDTEAEQELAAAFGIRSIPSILFCPVDGQPQMAVGVLP
                     KDSFKQAIEDVLLKA"
     gene            12319..12972
                     /locus_tag="PLF20_01660"
     CDS             12319..12972
                     /locus_tag="PLF20_01660"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HOX79751.1"
                     /translation="MDVLLSTAYFPCIEYLAVIGAADHIWIENHETYTRQTYRNRCYI
                     AAPNGRQMLIIPVIRPSGKHTFINEVLIDNKQPWQKIHWRSIETAYNRSAFFLYYKDE
                     LETLFIEPQYKLIEFNQKAVYKILKLLHLDKNLFFTETYRHKTGSEVLDLRNRIEPNR
                     QGLLSEYHFIDYFQPFGPKYGFLPNLSVIDLLFNQGPYSSDYLHKMAQYIKKTIENA"
     gene            complement(12977..15046)
                     /locus_tag="PLF20_01665"
     CDS             complement(12977..15046)
                     /locus_tag="PLF20_01665"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOX79752.1"
                     /translation="MRNPLRLFTDHLYYVSKGLLFLCALIIILLFFPREGKFKYEYVK
                     GKPWLHEDLIAPFDFSVLKTDEEYELQKERLLAKSRLYFTYQPEVTANQRVIVAQALI
                     DFLMMSPSDTLSGKPIVEVYRKAIQIADTIFRHGIIQMVPELIALPSGQSIMIVKGNI
                     ATEAYPEHFFTVQSADAYIVKHLSGIDSAYRRKVRQALEMGLAHNIRFDKELTEKSQR
                     QLLANLSRVKGLIQQGEKVISKGEVVDEEKFQVLESLRIETEKRLGTGHARFAVFIGQ
                     LILVSLAMLVLGLFLYFFRPHIFADNHQVGLILLIIISMVGLTSLVLSNKPLLLYAIP
                     VCMVPLIARIFFDTRVALYVHIITIILIGFLVPNSFEFIFLQLISGLITIFSVANFHR
                     RQQFVITAIYVFLAYSAVYFGLYLIQEGHFSGLKSFNFALFGGASALLLLSYPVVYVL
                     EKAFGMLTDITLLEISDVNYKPLKELSSRAPGTFHHSIQVANLAEEVIHQIGGNPLLV
                     RAGALYHDIGKMDMPMYFIENQYTGFNPHEELSPEESAEIILNHVRNGIEKARKYRLP
                     EQIIDFIRTHHGTRRVEYFYRISQQMELESEVNEQAFTYPGPVPFSKETCVLMMADSV
                     EAASRSLHQTDETHIETLVEQLIDSMMKAHQFDNSNITLKDISQAKKIFKQRLLHIYH
                     LRIEYPT"
     gene            complement(15188..16996)
                     /gene="typA"
                     /locus_tag="PLF20_01670"
     CDS             complement(15188..16996)
                     /gene="typA"
                     /locus_tag="PLF20_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006255979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HOX79753.1"
                     /translation="MTEIRNIAIIAHVDHGKTTLVDRILYQGKLFRENQQIGELILDS
                     NELERERGITILAKNVSVRYKGVKINVIDTPGHSDFGGEVERVLNMADGVLLVVDAFE
                     GPMPQTRFVLEKALEMNLKPVVVINKVDKPGCEPELVQEAVFDLMFSLGASNDQLDFP
                     TVFGSAKQGWMSDHWERPTQDVSYLLDTIIRYIPPPHKEKGSLQMMITSLDYSSYLGR
                     IAVGRIHRGTIQARQNVSVVRRDGSVYKSHIKELYVFEGLGKERIKTPVEAGDIVAVL
                     GPEEFEIGNTIADFENPEAIPPIKVDEPTMSMIFAINNSPFYGKEGKYVTSRHLRDRL
                     FLETQKNLALRVEETFQPDTYMVYGRGVLHLSILIETMRREGYEFQVGQPKVIIREMN
                     GEKCEPIEFLSINVPEEFTGKIIDIVTSRKGEIEGVCTRNNRAYLEFSIPSRGIIGLR
                     NAVLTATEGEAVMSHRFKGFETWKGDIPHRRNGVLIAMETGEAITYSLDKLQDRGTFF
                     IEPGQNVYAGQIIGENSRSTDLVVNVIHTKKLTNIRAAGSDEKAILSPPRIFSLEEAL
                     EYIAADEYVEVTPNAIRMRKIILDHLKRKRENKTEE"
     gene            complement(17106..17906)
                     /locus_tag="PLF20_01675"
     CDS             complement(17106..17906)
                     /locus_tag="PLF20_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79754.1"
                     /translation="MNEKKNKVSGIVATIVFHALFLLLMFTMALRTPLPLPEEEGVEV
                     NLGYSDEGFGSIQAAELPAGQRAAVIPLGSIKDEEIVTTNDEEIPSLSHKEKNIKKNK
                     QTTPLEQTGEKKSQEPVLNPNALYTGKLNGEGTVTNQGIAGGIGDQGRPDGTTGSNSY
                     TGTGGTGQGPGVYLSGRTKVFLYQPAYNSMEQGTVVVEITVDRQGNVIRAIPGKKVPN
                     TSIGTNTTDQELWKAAREAALKSKFSSNPNADPEQKGYIVYHFVKLEG"
     gene            complement(17903..18346)
                     /locus_tag="PLF20_01680"
     CDS             complement(17903..18346)
                     /locus_tag="PLF20_01680"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOX79755.1"
                     /translation="MAIQKRNKLNIAFNPAPMSDLVFLLLIFFVISSTLVSPNAIKLL
                     LPSSKSKTMAKQAITVYINDHFEYFVDPDGRGHAVPVNLPQLQQILAENLQKQDIDAS
                     VVLRADQSVPVQYVVSLFDIVNDINTQLNSKYKVILATRPVKEIQ"
     gene            complement(18358..19080)
                     /locus_tag="PLF20_01685"
     CDS             complement(18358..19080)
                     /locus_tag="PLF20_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOX79756.1"
                     /translation="MLFLLAALQDTPVMKSLQQLPQNVGSTVPQAQQISLSVWDLAVK
                     GGWIMVILGIMSIIAVYIFVERYMAISRASKEDKNFMNHIRDFIHNGRVDAAVALCRA
                     QDNPLARMIEKGLLRLGKPLNDINASIENVGQLEVSRLEKNIAGLGTIAGAAPMLGFL
                     GTVAGMIRAFYDMSMAGNNVDLIVLSSGIYEAMVTTVGGLIVGILAYVCYNVLVARIE
                     KLVFNLEARATDFMDLLQEPAA"
     gene            complement(19246..19905)
                     /gene="plsY"
                     /locus_tag="PLF20_01690"
     CDS             complement(19246..19905)
                     /gene="plsY"
                     /locus_tag="PLF20_01690"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOX79757.1"
                     /translation="MLITIVLIIIAYSIGSFSSAVWIGKWFYGIDVRRYGSMNAGATN
                     TLRVLGWKAGIPVLLLDALKGWIAVKLAAWLMPKPLGELQQELLLIACGIAAVLGHIF
                     PLYSRFKGGKGVATLLGIGLALFPYPTMGAVVVFIIFMILSCIVSLSSILAGISFPFL
                     VVFLPYQPRPHWPFLVLSILVAIFIPFTHLSNIRRLLKGEEKKFSIAKNAPRKEINLE
                     K"
     gene            complement(19915..24474)
                     /locus_tag="PLF20_01695"
     CDS             complement(19915..24474)
                     /locus_tag="PLF20_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79758.1"
                     /translation="MKRIVFLVTLLFFQSLCLFPQLNEQEAFWLRVDALFSKIELKNK
                     DKEVIFSGQVFVNELKANWQSHYELDEMIPQVNSLVEKMLERRLKPYEHIAPFLTTIV
                     NLAILNAEQGQVKPFLDGVSYLLDQPGPSQLNDFYHISNSLLRDSLLSRYKNINWKVS
                     QIRFEIINYPTPRFKFKDIDLICQMGSSRRKIYQTLGIYDPFSREWKGVGGQTPWVNN
                     NIPSDSVYVEFDRYGIFLQGTDIQADSAMLYHKTYFPSGVMGKFEDNFKPGNKPGLPR
                     FVSYDRNIKIPGISQNVDFEGGVKLETHQLNGIGESGRPARLFFYTPQKNRVVIKSQQ
                     FFFKNPFISALDANATIRYRFDSIFHPAISFVYDENKRQLNLYQGNSILSSLPFFSSY
                     QRMEIQANTLLWNIDDSLMIFKKGAGLVRENDAVFISENYFRGEDFRTLQGIDPVNPL
                     IRLYQLAKQLHKSQFGLNEYASAIHLSADQAERLALQMAARGFLLYDFEQKEIILRQK
                     LFHWVDSYYGDIDFDNLVILSSKTDTNAILNLHNLDLQVFSVDQVILSDSQKVMVTPY
                     NYTITLKENRNFAFSGRTKAGYFDFYSNRRNLFLYEEFQLRLPEIDSIQFMAIDIPKS
                     SKTGKINPKLVKIESQIEQVSGTLQIDHPQNKSGRKNLKIPYPVFKTDSTPSYVFYDR
                     KGRYKTQYPHKSFYYKVKPFLLNNLDNYYLDSLNLKGTLYSANIFKVMEEPLIIRPDY
                     SLGIDIHTGKSGEAIYLHGGQPKGWFAGRLDLSHQGFRGDGQLNYLQSISITDTTNRT
                     DSTDFVFFPERAQASVLSFSITESSEDIEIPSVKGRRIKEDWYPYKDLMNLKSIKNPF
                     SMYNGMMAFEGNLMLKPDGLDGQGKAGFDKASLNSDFYQFRRRTLKADSSQFLLNTPD
                     EKAVAIRAVNYNAFVDFDSARAVFNAGRIARIDFPVFSCYSNHSQFLWDMNQKKIQLQ
                     SPDFDSQKQYMAFQKPNELITAFEENTLKGPTFVALQPNMDSLQFISFSGSYEIQNNL
                     LNFTDIPFIKSADAYIFLSEKQIQITPQNLPVSFKNARLITSRNDIRREFSHVNGEII
                     SGKKFQAKGTTYFSLDRQEKTSLIFFDQIKPDPFTSITHGSATVNDTSSFILDPWFDF
                     YGQVDFSSDTKPLLFEGYYRILQSGCGLSASWIRFDTLVEPDRVILPLPARCFDPTGI
                     ELVTGIFMNRTGGKVYARFLEPLLDPRDLSVFTASGKILWDQNENKYKVVKNQNSEIF
                     NMDNPLFEYHPEQCKIMVHGLINLHPRFSPVSRIETTGNAIYDLKTDSLRMNLLFAFD
                     FPFFEKVWKIIVNELFSLDNPLVNADDKRYKEQLLALFGEKGYTQMINEVQSGKTIST
                     VVGNSILMTDIHFVWDSVEQVFNYRGPISLLYIKGNYIGRKIPGYIQLKHQENAREDQ
                     LRILLRPDEDHYFYIDYGRGNVLRVYSTNKEFTDLVVKERESFNKQMEKDNPAKNWQP
                     FRVAPVNRAEAIDFRAEMEKVKLFN"
     gene            complement(24847..25683)
                     /gene="prmA"
                     /locus_tag="PLF20_01700"
     CDS             complement(24847..25683)
                     /gene="prmA"
                     /locus_tag="PLF20_01700"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOX79759.1"
                     /translation="MEYTELRIPIIIDDETKELLPSILTELGFEGFIEEEDSLLGYIP
                     SVHFDRHAVEELLQRLDIAPFFTFRTVPEQNWNAVWESQFEPVVIAEKCFVRAPFHFP
                     QPHYPIELIIEPKMSFGTAHHETTAMMMEYLLEIDVMGKSILDVGTGTGILAILSAKI
                     GAVNIVAIDNNEWAYRNALENIQLNNTPRIVVKQGDASLLSTMNEFDVILANINRNIL
                     LNDIPAYMKVLKQNGLLIVSGFYKDDLPVIDDAFHRVGLASCRIKERNSWLAAVYLKT
                     LP"
     gene            complement(25690..26451)
                     /gene="tpiA"
                     /locus_tag="PLF20_01705"
     CDS             complement(25690..26451)
                     /gene="tpiA"
                     /locus_tag="PLF20_01705"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOX79760.1"
                     /translation="MRLKIVAGNWKMNLDFEEAEDLISAIADELENIDLGHAEVVICP
                     PFPYLEMALDIAIESVFSVGAQNISHFDNGAYTGEVSARMLASMDVDYCIVGHSERRK
                     YFNETDEIIAKKIDQAIEHEFITIFCCGELLSERQAGKHFDVVREQIEKAVFPLSEKE
                     FYNIVIAYEPVWAIGTGVNATPEQAQEMHAYIRNLIASRYGKDVADETSILYGGSCNS
                     KNAPEIFAQPDVDGGLIGGASLNADEFIEIVKAIL"
     gene            complement(26906..27730)
                     /locus_tag="PLF20_01710"
     CDS             complement(26906..27730)
                     /locus_tag="PLF20_01710"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOX79761.1"
                     /translation="MRKKVFSLVAALGFIMVIIPAFGQSNVISEIRQVGSFQTIQNNS
                     SVDILLLQGNSFEVKIEADSEIISYIDTEERNSTLYIDVKKRPGLRKIKVMRAIITVP
                     NLNVIRINGSGDVVSMNQLNFPRFSITLNGSGDAELELKCKEFESYLNGSGDLLVEGV
                     NGSCEIHVAGSGDMKGKNFQLSNLKITNRGSGDIKLSGKADEVNIYSNSSGDIHCFEL
                     PCNKAEIHCYGSGDVSLWVIQSITGSIHGSGDIRIKGNPNNRNIQKQGSGDIIWSN"
     gene            27855..28598
                     /locus_tag="PLF20_01715"
     CDS             27855..28598
                     /locus_tag="PLF20_01715"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOX79762.1"
                     /translation="MALRHSFEKTGNFFFRYRGQIPVIIFISLIPAMFFTDYSNLDPS
                     IAKCLTAISIVLAATGFLVRIITIGTTPAGTSGRNTKSQVAEKLNTSGIYSIVRHPLY
                     LGNYLIWAGLTLFVFNFWYFLVISLVFWIYYERIMFAEECYLERKYDQEYIEWTLKVP
                     TFFPSFKNYDPSALPFSFKSVLRREYSGMLSIVFGFAFIDYLRYYLLNRNFLSVRPSL
                     YILVAALIIALVLRTIKHHTKWLEESGRT"
     gene            29212..29376
                     /locus_tag="PLF20_01720"
     CDS             29212..29376
                     /locus_tag="PLF20_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79763.1"
                     /translation="MAMFLKESIFMDRDLLENILKLDNDEIELQLEEYGELEGRLPKK
                     HKRYNIESFI"
     gene            complement(29564..30871)
                     /locus_tag="PLF20_01725"
     CDS             complement(29564..30871)
                     /locus_tag="PLF20_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79764.1"
                     /translation="MTKKILILPFLFLGSFFYSQAQDSIINQLLRTRQKDYSNYIQQR
                     DSIKVRTWEKVVMLNGLAARVIQSDSILIAACLEQLAAGIPSVREQQLLDTITHLKGR
                     IEYMKQSSSPENDLSPRDYVILLGVSLILILLGIFALILLRKLQKANKSGLKTKEELE
                     TFQNTQLEYENKLEEINDELARIYNEREQLLQQITELNRALISEKQNNNQLKDCLETI
                     SNEKQEMQNEMKRLKEKAEKYQNLPDESTINEIITENNLLKSENIQLKQQILELQENI
                     EQLKSELLKVTESSDELLRKSLEEREQKYKMELQNAQQIQLQLEDKIKEIEERLKNIL
                     EEKDILVSEHNFVMESLSSMCDELERSKAQLQSVETEKISLEEQLVNLSPLPQQIDAL
                     NAEIIRWQNENEELKQQIAREIKLRKEIESDIQKIFNRLEGGV"
     gene            30993..32453
                     /locus_tag="PLF20_01730"
     CDS             30993..32453
                     /locus_tag="PLF20_01730"
                     /inference="COORDINATES: protein motif:HMM:NF028437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5106 domain-containing protein"
                     /protein_id="HOX79765.1"
                     /translation="MRNVLFILAVSLFSVLSEVNAQGFQLKVKIKGANDDFLYLAHYF
                     ADKQYMDDTLRKGKGEWFVLEVDSMLTGGLYIIAGEKKNRYFEFLMNNEPRISFETIA
                     DDPVNNMQIKGSSENLAFFRYIHYLGKKQKEIEPYQKIFKRTEGLYPDSASLAKAWMQ
                     KINDEVMSYIKEFIESHEGMFAADFVKASQEPEIPDPPLLPNGRPDSLFAYKYYKAHY
                     FDNLPLNDARLLRTPFYHQRVNTYFTKMVLQHPDSIIAEAKRLIPIASQSADTYRYMI
                     WFLTNFTERSEIMGMDAAFVYMVENYYASGEMDYWINNTVKENLIKKAKKLKPILIGQ
                     TAPELIMLDTFMQPISLHQLKARYTILLFWDPTCGHCKKEIPMLKDFMTRKGKQYDIE
                     VFAVCSDTNMREMKNYIRQYDMDWINVNGPRAYTANYHDLYDIYSTPIIYLLDEKKTI
                     LAKRLTVEQIESFIEHHSRSESGGVLQMKQEYRNNK"
BASE COUNT         9897 a         6377 c         6234 g        10058 t
ORIGIN      
        1 accaatcact aaaaaatcag tatgtacacg catgatgata tttttcgcaa atttactcaa
       61 tttaaacttg aacatggttt aattttcata actaccttgt acattaaagt cctttatttc
      121 tctttggata ccacaaattg acaactaata ctaactcact gtgtgatttg gaatttggta
      181 tattgttagt tgtaaattga aaactttaaa ctgttgataa ctgctaactg aaagcaaata
      241 aactgccatt catttttatt tttaagagga aacaatgatt gcttgatttt gagcgtttat
      301 aaattcgttt aactttgcag ggagttttga agaaagcgtt catgcatata attcaaaatt
      361 ccttgataag attggatttt gttatacata taaataagtt ggaaaataat tgaaaaaaac
      421 agctattttc atgaaacaag agattattga taatataaat aatcctgaaa atttagaaaa
      481 attatatcgg aacaataaac aagccttcat taaatcattt gaagagattt cagatgatta
      541 taattcagat ttggtcaggt tttggaaaat tcgattagct tctgagaaag agcctgcgtt
      601 taagggtttt ttaaaatctg atttattagt cgttatatcc ctttcactta ttacggggtt
      661 attagttaag ttgcccgaaa ttttttctca aatacaaagt gagttctttt atacccgaga
      721 tttggccata attgtattca atggattaat tctttataca ttttggcaaa accggctttt
      781 taagggaaaa ccacttctta tctatagttc tattatagtt ttattaactt tatttgtaaa
      841 ttttttacca aatacccaga gcgattctgt gatgctttca atgattcatg ttccattgtt
      901 tttatggtgt ttattcggtc tgtcattcgt atcatttgat tacaaaaaca tccaaagacg
      961 gattgaattt attcgtttta atggagaact cataatcatg actggtttaa tcttaattgc
     1021 cggcgggctg cttaccttga taaccataca attattctca gtaataaaaa tgaatattga
     1081 aaacttttat ctacattata tcgctgtttt tggaagtgtt gctgctccta tagtttcaag
     1141 ctatttaatc aaattatatc caaatcttac caacaggatt gcacctgtga ttgcaagggt
     1201 attttcaccc ttggttttga ttacgcttgc ggtttatttg gtttcgttaa ttttctcaaa
     1261 aaataaaata ttagaggata gagatttgtt gatattattg aatataatgc ttcttgctgt
     1321 aatggctatt attgtttttt ccgtgtcgga actcgataaa tcaaaagaaa aaaatattaa
     1381 tgttcttata ttgattttat tagccgcttt ggctattgta gtaaattcca tcgctttaat
     1441 tgctattatt tcaagagtaa ctatgggatt aacacccaat agaactgttg tattgatttc
     1501 aaatatcttg atttttatta acttaatatt aattacaaaa aatctctttg aatcatatat
     1561 taaaaacgaa ccattagatt cggtcgaaag cactgttgcc aagtatttga atatttattt
     1621 ttattggacg attatcgtaa tatttgtttt gccttttgta tttggattaa aataagtaac
     1681 atttgctaac cttggctttc acaacattta gagtcaaagt aataaactat gcttcgttct
     1741 tgattgcaag gcttgtgcac ggatgaagga agaatgtcca cggtgtctgc atgttcgcat
     1801 agcctcttca gtttgcgttt aaggtcagaa ctcctgattc ggtgttaatc aggttgtcgc
     1861 agttttgaag acaagtgtca tatttgtacc ggggaaaatg gtttctgtgt cgttcaataa
     1921 ctcacagact ttttgaactg ccaggttatc ttttttagtt gccagagaat aaagtttaac
     1981 ggttagagtt ttatttactt catcgggaat caaatctgct ttcgaaaata ttacactctt
     2041 taccagttaa aacctaacag gtttccaaaa cctgttatgt ttggacaaga ccctatatat
     2101 tcttgacgtc ttagcgagaa aaatattgca cacggaggcg tcaagaatta aattaatttt
     2161 tcttgatgtc gctacaatct gtcaggttaa cctatctaaa aatttttgcc agaaaatacg
     2221 cccaattgaa attcggggtt aattttgtcc cgtcataact aacgggagtg atatgcaatt
     2281 ataggatcag aagttctgag attgataaat aaatagttgc aataaaatag atgggatgac
     2341 agcatcaaaa aaggcagctg tggctgcctt ttatagatgg aagattgaaa ggatgaaatt
     2401 attcatccac gaattttacc ccagggagtt ctttgccttc aatatattcg agcatggcac
     2461 caccgccagt ggaaacataa cttaccctat cggccagttg atatttattg atcgcagcta
     2521 ctgaatcgcc tccacccact aaggaaaaag cgcccttggc agtagcctcg gcaatgcact
     2581 gtgcaatagc cactgtgccc gtttcgaaat tactcatttc gaaaacaccc ataggtccat
     2641 tccaaagcaa ggtcttcgaa tgagtaatca cgtccttgaa gagttcgatc gtcttgggac
     2701 caatgtcgag gcccatccat ccgtcaggaa tttcatcggc tttgcaaacc ttacgattgg
     2761 cctcattatc gaatttatcg gcaattacta tatccaccgg aagaaaaatc tttataccta
     2821 attgttttgc cttttcaaga gtttcgaggg caagagaaat cagttcatct tcgacaagtg
     2881 aatttcctac cttgcctccc tgagccttga taaaggtgta tatcattcct ccgccaataa
     2941 tcaagttatc gacccttttc atcagattgc gaattatgtc gatttttccc gagactttgg
     3001 ctccacctaa aatggcagta aatggatgtt cggcttcttc gagaaccttt ttcagagcgg
     3061 ccagttcgtt ccccatcaca taaccgaaca tttttgcttg tgggaagaat ttggcaataa
     3121 cagccgtaga agcatgcgcc cgatgggctg caccaaaagc atcgttcacg taaacatcac
     3181 ccaatcgagc taattttctg gcaaattctt cgtctccttt ttcttcttcc ttgtaaaaac
     3241 gtagattctc taaaagtaaa atttcgccag gtttcaattc taatgccatc tttaacgcat
     3301 cctctccaac acaatcggga gcaaatttca cctctacacc cgtaaccttt ctcaagtaag
     3361 gcagcaaatg cctgaggcta tatttatctg atggtccatc ctttggcctg cccaaatgcg
     3421 acatcaaaat cacgcaacct ccatcattca gaatttttcg aatcgtaggt aatgtaacat
     3481 cgattcgggt ggtatcggca atttgaaatt tatcatccag cggaacatta aaatcgactc
     3541 gcataagggc ccgcttattc ttgaaattaa aatcatctat ggtcttcata tagtaatatt
     3601 tttaaaatta ggccacaaag ataagatttt tagagcattc aattgttgtt gaatatatgc
     3661 aggccggtgc cttctatgac cacttcgagc tggttttcag aaattgttct catcaccttc
     3721 cggcagaatt aatattgtat tgtctgcatt aattatcgaa aaggaaagat gaagaagaat
     3781 tttcgatact ttttattatt gtgctgtcaa ggctctgagc aggaacaccc attcgaaggc
     3841 aacttcttta agagcatcat atctgccggt agcccctcca tgacctgatt ccatgttggt
     3901 tttcagaagt ataatattat cgccggtgtt gttggctctg agttttgcta cccatttggc
     3961 aggctcatga taacgaactt gagaatcatt gagtccagcg gtagccagaa taggcgggta
     4021 attagccggt tttatattat cgtaaggaga atacgataaa atgtagttat aatctttctt
     4081 tttattggga tttccccatt gttcatattc ctgagtggtc aagggtaagg ttttatcgag
     4141 catggtattg attacatcga caaaaggtac atcggcaacg actgcgccaa agaggtcggg
     4201 ttgcagattg agcgcagcgg ctaccaatag tccaccagca cttcctccca tgataccgag
     4261 ttttttaggt tgggaatatt ttttatcgat aagaaattgt gcacaggcaa caaaatcggt
     4321 aaacgaattc ttctttttca tcaattttcc atcttcgtac cattgttcgc ccatctcact
     4381 tcctccacgc acctgagcaa tcgcatacac aaatccccta tccaccagac tgaaaactga
     4441 ggtacggaac catgcatcgg tgctgtaacc ataagctcca taaccataca acaaggcagg
     4501 attcgaccca ttcaattcga gtcccttcct gtataaaatg gccatgggca catctgtacc
     4561 gtcagaagct ttggcaaatc tacgttccac tgcatactta tcaggatcaa aacctgaagg
     4621 aatttcctga accttgagcg tttgcacgct atggctatcc atatcgattt ggtatatggt
     4681 ggtcggtcga ttgagcgatg aataggaata cctgaatacc ttctggctgt actcaggatt
     4741 cgtataagtg gaaaccacat aaaccggttc ggggaaagcc acattatgaa acttgcttcc
     4801 gtccagatcg taaattctca atcccgtcaa tccattgctg cgtgtctgta ttaccagatg
     4861 attctcgaag acctcataat tctctatctt taccttttca tcgtgaggaa agataacttt
     4921 ccattgtttc ggattggagt ttttgccgaa aggtgttaga taaaccattc catttttact
     4981 ttcgggagat ttgtaataga catagaaccc taaattttta tgatgttcca caccatacat
     5041 gacatccttt tttcgtggca aaaaaatctg gaaattcccg tcgggttgat cggattcgag
     5101 cattcttacc tcagaagttg taaagctagc cgattcaatc aatatataat cgtttgacaa
     5161 ggttttggat acggaaacat tcagcaattc gtcatcttcc tcgaaaacag gagtatcttc
     5221 ggcgagagtt cctgcaatat ggcgtaaaac cttatatggg cggagggatt catttccaat
     5281 ggtatagaat aatgttttac tatcggctgc ccaagtaaag gactgaacat tttcgatctg
     5341 ctcgggtaaa tcttctccac ttcttagatc tcttatctta agggtaaatt cggcaaacga
     5401 accggttgtg ttataggaat aagcggcaaa attattgtca gggctcactt cataatcact
     5461 aaaaataaaa gctggctggc tctcggccat ggcatttacg tcgaaaatta gctcttcttc
     5521 tgcttgtgga gtcatttttt gacgataata caagggatat tgctttcctt taagcgtacg
     5581 cgtaaagtaa taataaccgt tgtcaggaat ttttacgctc atatcttctt ctttgatacg
     5641 cgattttatt tcattgaaga tttcttcctg gagaggttca gtttcttgca taaccagttt
     5701 gcaatattca ttttcggcgt tcaggtaagc aatcacctcg ggattattgc ggtctttcag
     5761 ccagaaataa ggatcgattc gtgtatttcc aaactcacga aattctacag ggcgcgaagc
     5821 ggctataggc ggtgcaggat aaagatattg cttcaaagga cgcaaatttt gctgagcgtc
     5881 gagttgtaag tgtgtcataa agatgaatgt aaataaaata gtggtgaatg gataatgaaa
     5941 tgttttcatg atgaattgaa gattatatgt gatgcattgt tgcagtgagt ttttcactaa
     6001 aagtataagg cgtcgttcgc tttcaaaata ttttgttgca aagaaacgaa aatctataat
     6061 ctataataaa gtttgcagag atgaatcttc gactttcaag gatgccaaaa agttacactt
     6121 tccgattatt ttcttataat tttattgctt atcaaaataa tccatgcaag ctggcattta
     6181 ttttttcgat gacgctggca aggtcttcgg tattagaggc aaaatcggta tgatctacat
     6241 ctatgatgag aagttttccg gctttatatt gtgaaatcca gtcttcatac cgttcattca
     6301 atcctttgag gtaatccagg cgtatgcttt cctcgtaagg gcggcctcgt ttctgaatct
     6361 gacgcacaag agtcggaacg gtagctctta aatacactaa aaggtcaggg ggttgaatga
     6421 atgaaatcat cagttcgaag aggctttgat aattttggaa gtctctggta tccataagcc
     6481 ccatgtcatg cagattgggg gcaaaaatgt aggcatcttc gtatattgtt ctgtcctgaa
     6541 ccacattttc acccgatttt ctaatttcga cgatttgacg aaagcggctg ttgagaaaat
     6601 agatttgcag attgaatgac caacgctgca tatcttcata aaaactcatc aaataagggt
     6661 ttgtatctac attttcgaaa tgaggtttcc attgaaaatg ttttgcaagc aatgcagtga
     6721 gtgtactttt tcccgaaccg atatttcctg caatagctat gtgcataagg cgaaattttt
     6781 aataagataa attctataga atgagttaat ggtttgtttt agtggtaggt tgctgaagca
     6841 tttcgagaat ttcttcgatg atatttctct cgttggctaa gcgaggaacc ttgttttgtc
     6901 cacctaactt gttgcgattt ttcatccatt gatagaatgt tcctcgaggg acttgctgaa
     6961 tgataggact ggataacacc atatcatgga atctcttagc ctcgtaatcg gaattcaccg
     7021 attgaagggc tttatcgaaa gtttcgacga agcactcgaa atcttcgggt gttttttcga
     7081 attctaccag ccactggtga gcgccatttc tattttggct aaaatagacc ggtgcgcccg
     7141 tatattcgct caattgagca ttacattctt tacaagcaat ttcgaaggca cgttcaacat
     7201 tttcggctat tacttcttct ccgaaggcat tgataaaatg gcaggtgcgg cctgtaactc
     7261 taaatttgta tggattctga cgggtgaaaa ttatagtatc gcccagcatg taacgccaca
     7321 atccactatt cgtagaaatg agcatggcat aatttttccc taattcggtt tgccatagtg
     7381 gtagagcata ttcgtcaggt tgatcgattt tatctaaagg aacaaattca tagaatatgc
     7441 cataatccag catcagcagc atggccttct ccgacgaatc atcctgaata ccaaagaaac
     7501 cttctgaagc attgtaggtt tcgacataat ggaggtgttg gagtgagatg agtttttcga
     7561 actcttgctt ataaggtcta aagctgactc ctccatggaa aaaaacttcc aatcgaggcc
     7621 ataactcacc aatatgttgc ctgttcgtat cttcaaggat tcttttcaac aatacaagcg
     7681 tccagctcgg tacaccaatg atgctggtaa cattttcttt gatggtaatt tgggccatct
     7741 tggcaatttt ctcttcccag tttttcatca gggcaacctt aagttctggg gtgctccttt
     7801 ttgcagccca aaatggaaga ttttgcatga ggatagctga aagatcgcct tcgacaattt
     7861 gatgatgatt cacccggtgc aaggtttgcg aacctcccat tactaaactt ttccctgaga
     7921 aaagacgggt ctgaggataa tgattcacat aaatcgacag catatcctta cctgccttaa
     7981 aatgacaatg atacaaggat tctttgctca aaggaataaa cttgctctta tcgttagtag
     8041 tccccgatga tttggcaaac cacctgatag gcgtattcca taatacgtta ttctcgcctt
     8101 gcctaactct ttcgatgtat gattttaatg attcataatc ctgaacaggg actttctttc
     8161 taaaatcttc ataattatgt attcgttgat aatcataaac tttcccccat tcggtatttt
     8221 tggcagatgt aatcaatttc tccaaaaccc aattttgaac ttcatacgca tgaagctcga
     8281 aataattgat tcgctcgatt cttcttctta aatacctcga aattagagtg tttgtcaatg
     8341 acatcctttt atcccgaatt ttcagtcaaa agtaatgatt cctgcgatta tataaaaagt
     8401 aaaatttctg gctcaaccat tcgacaaatt acagttgaac tcattattta ttcgaaatgc
     8461 acagcatggg tacggttctt cactggaaat tactttctta cccaagctac ctgtaaaagg
     8521 gcttcgcttc aacatccata agcctgctat taaatttatg ctgatttgtg attctaaaat
     8581 tcttattttt gcaatggaga ttctttcggt gaattacagc ccagattctg taaaattcaa
     8641 ttgctctaag aaaagagcat caagttagta ttcgacgggc tggcccgaat gaaggattat
     8701 tatacatatc gagtgaaaga tttttacaat tgtaaagcta aacagcaaat tttgatttga
     8761 aatatacatc gaaaaatgat aactgaagaa agtcttcgac aatttggagt tttgattcac
     8821 gaggtgagga atgtaagttc ggatgaggca ctggctcttc tatctgatgg ggctgttata
     8881 ctggatgtaa ggccattgtt cgaatcggaa tatcgtagat tcgacgtaaa ggatgtaatt
     8941 tacgcaccag ccgaaggata cgaatattgg gtaaaaaatt tgcccttcga tcgttgctta
     9001 attgtagccg acgccagtgg cctaaaaagc agaaccattg ccagtcgttt acttgccgat
     9061 ggattccgga tggtggtaaa ccttagtgga ggattggtag attgggaaag aagaggtgca
     9121 ccggtaattg tggataaatc gaagcaactt atgggttcct gcctttgcca gcttaaacca
     9181 ttctattata aaaaaagaag ggaatagttt aaattcatta ttatacagga tagtttttta
     9241 gcaagtattg ttatggttta attgtttcga ggcatgagcc gcgagaagaa caaaagtcaa
     9301 aaaagacgcg aggtgaaggg aatagtgaac agagtttgga aaatacaaga tatgagcgat
     9361 cttctatacc aaatacacaa attatttttc aatattgcca gaaccatttt taaatgtgac
     9421 ggattctttt acatttagga tttttggaga taaaacggag ttccataaag aaactcaaat
     9481 aagtttattt atgattccaa taataaattt tatttcttca agtgatttaa attgtcgatt
     9541 cttttgtatt tatttataaa aaaaatggtt ctggcataag caaactgctc gactataaac
     9601 ccgatgaaat aaaaactcaa attattggca aggtaagaga tacactttta ataacaaaca
     9661 taaaccttat caatttaata attccaacga tatgatgtta tatacagttg atttaaaaca
     9721 tgatgaacaa atagataata ttaaactttc agtaatagct ttttaacaac ctggtcaatt
     9781 gtttcaaaaa taaattgtaa atagaggctc atttatggtt tataatttta agggaaaaac
     9841 tctgccacta aaaattaatt tagcattcaa taatctattt tgataattaa tatattaatg
     9901 tcctaaaata gaattcatta tgcaaatcca aaattaatat agttttcgaa taaataaaat
     9961 aaagtaaaat atttttcaag ataaggatga aaaaatcatt caattatgtt gtgcaatcaa
    10021 tttatacagt cattttcgac gatggaggtt ttgatgaagc ggtttgaaaa cttattaaaa
    10081 attctttaaa cctgattcca ggcattccac aattttcttc ttcagtttcc agcaagtcat
    10141 ttgtcgtaga ttaaaatatc tggccaattc atagctcgaa attttgggat tgtggcaaac
    10201 gatattggca aatcgaaagg cttccggcaa aggtaaacgg caatagtgaa aaattgtatg
    10261 ggccttggcc gattcttcac gtttacatcg cgtacaccgc ctcgaatatg gtgtattccc
    10321 cttacaataa ttgttatgcc cgcaattgcg acaaatatat ccgccttccc attttttcaa
    10381 ggctaagtat tccaagcaag attcctcact ggcaaatttt ttgcagatgc cattaaaatc
    10441 agtgtgatga aatatttcaa caattccatc catgacaaca tgtcatattt aaagttccaa
    10501 atgtaaaatt ttatctactc gcagtttatt caataaaaac tctattcgtt cgaacataaa
    10561 tgcatttaat ttgtttttgg gatgttagtc tcatatcggt ctaatctttg caaagataaa
    10621 aaagtgataa aaatgcgatt tgattacaag aattcgttta attttgctac ctcaaaacca
    10681 agtgtcgata tgaagaaaat ttttctcttt acgggaattg tttttatctc tctgtcattc
    10741 gtttcatgtg gaaagaatca aaagactgaa tcgactgaaa gcaaatcgaa ggaaacaact
    10801 tctcccgtac agataatcaa gctcgacaat ggactttttt gcaagacaat atgggattac
    10861 acgaattcac ccgatcgatg gaaatataga ggtaacaagc cgagtgtcat agatttttat
    10921 gctgattggt gcaaaccctg taaaatcgcc tctctcattc tcgaagattt agccagggaa
    10981 tatgcagggg aagtgaattt ttacaaaatt gatactgata gggaaaaaga attagctact
    11041 ctatttggga taaggaccat accttcgttt ctttttattc ccaaagaagg cgagccccgg
    11101 atgacagccg gtattgcccc aggggaagaa gctacgcgta aaatgttcaa cgattatatt
    11161 cagcaatact gtctgggaaa ttttagcgaa gcacaatagt ttaccaattg aatacaaatt
    11221 tgatatacca atccaaaggc ctggaataat gaaaaaaata aatttaaaac tcatactcac
    11281 catttttgtg ttcaccttct cgattctgga tttttttggt caagcatcaa cgaaaaccat
    11341 ttcaaacgaa gggaaagtaa tttatctaac ttcagccgag tttaaagaaa aggtatttaa
    11401 ttgggaaaaa agccctgaaa actgggtatt tgaaggcgat cggccttgta ttgtagattt
    11461 ctatgccgat tggtgcaagc catgtagaat ggttgctcct attatggaag agttagcctt
    11521 aaaatataaa ggtaaactaa ccatttacaa agtgaatacg gataaagaaa aagaggtctc
    11581 gagcgtattc ggtattcgca gcattccctc ggttcttttc tgccccacga aagggaagcc
    11641 acagatggct acgggtgctt tacccaaaga cacttacatt caggttatcg aggagtttct
    11701 tctgggaaat aaaaaacaga cccgataaca agaatctaaa taattacaat tcattacggt
    11761 aaaatacata aactcatttc tattctatgg aaaatttaac gaaacagacc tttctcgaaa
    11821 aagttttcga ttatgaaaaa aacgatcaat ggaaattcga gggcaaattg ccatgcatca
    11881 ttgattttta tgcagactgg tgcggccctt gtaaggtggt agcacctatt ctggaagaat
    11941 tatcgaaaga atatgctggc agggtgaatt tttacaaggt agataccgaa gccgagcagg
    12001 aactggccgc agcttttggc atcagaagca taccctctat tctgttctgc cccgttgatg
    12061 gtcaaccaca aatggcggtg ggtgtattac caaaagatag cttcaaacaa gccatcgaag
    12121 atgtactttt aaaagcataa aatatctttc tatttgcaac aacccgcata aagattcaaa
    12181 tataaatcgc atgcgggttt tttattgtgt tcgacactga tatggaaata aagagacaca
    12241 ttatggaatt ctctatattt tagcattttc gataatattg cctatcgaga aatatctttt
    12301 taaaaaaaaa ttcgagaaat ggatgtgttg ctgagtactg cttatttccc atgcattgaa
    12361 tatttggctg tcataggtgc ggccgatcat atctggattg aaaatcacga aacgtacaca
    12421 cggcaaactt accgtaaccg gtgttatata gcggctccaa atggccgtca aatgctgatt
    12481 attccggtta tcaggccttc aggaaaacac accttcatca atgaggtttt gatcgacaat
    12541 aagcagccat ggcaaaaaat tcactggcgt tccatcgaaa ctgcctataa tcggtcggct
    12601 ttttttcttt attacaaaga tgaattggaa actttattca tcgaacccca atataaatta
    12661 atagaattta atcaaaaagc agtttataaa atattaaaat tacttcattt agataaaaat
    12721 ctctttttca ccgaaactta taggcataaa acaggatcgg aggttctcga tttaagaaat
    12781 cgaatagaac ccaaccgtca gggcctattg tccgaatatc atttcatcga ctattttcaa
    12841 cctttcggcc caaaatacgg ttttctaccc aatctgagtg tgatagatct cttatttaat
    12901 caagggccat acagttctga ttatcttcat aaaatggctc agtatatcaa gaaaacaatc
    12961 gagaacgcat gaggttttag gtgggatatt ctattcgaag atgataaata tgcaacaggc
    13021 gctgtttgaa gatttttttt gcctgactga tatccttcag ggtgatgttg gaattatcga
    13081 attgatgcgc tttcatcatc gaatcgatca attgttcaac gagggtttct atgtgggttt
    13141 catcggtttg gtgtaaactt ctcgaagcgg cttcgacgct gtcggccatc atcaatacgc
    13201 aggtttcttt cgaaaaaggc acgggacccg ggtaagtaaa tgcctgttca tttacttccg
    13261 attctaattc catttgctgg cttattctat agaaatattc aactcttcgt gttccgtgat
    13321 gggttcggat aaaatcaatg atctgctcag gcagcctgta tttgcgtgct ttttctattc
    13381 catttcgaac atgattcaaa ataatttcag cactctcttc gggtgaaagt tcttcgtgag
    13441 gattaaaacc agtgtattga ttttcgataa aatacatcgg catatccatt ttcccaatat
    13501 cgtgatacaa agcccctgcc ctcaccagaa gaggattccc accgatttga tgaatcacct
    13561 cctcggccag attggctacc tgtatggaat ggtggaatgt tccgggtgca cgtgaggaaa
    13621 gctcttttaa aggtttataa ttcacatcgc tgatctcgag caaggtaata tcggtgagca
    13681 ttccaaaagc cttttcgaga acatatacca ctgggtaaga aagaagtaat aaagcagaag
    13741 ctccgccaaa aagagcaaag ttgaatgact tcaagcctga aaaatgccct tcctgaatga
    13801 gatacagacc aaaatatact gcagagtatg ccagaaatac ataaatggca gtaattacaa
    13861 attgctgccg cctgtgaaag ttggccacac taaatatggt aatcaatcct gagataagct
    13921 gcagaaagat aaattcaaaa ctattgggca caagaaatcc gatcaggata atggtaataa
    13981 tatgtacata aagagctaca cgcgtatcga aaaatattct tgcaatcaat ggcaccatac
    14041 aaaccggaat ggcatagagc agaaggggtt tgttggacag gaccaatgaa gtcagcccga
    14101 ccatcgaaat gatgatgagc agaataaggc ctacctgatg gttatcggca aaaatatgag
    14161 gcctgaaaaa gtacaagaac aagcccaata cgagcatggc aagactaacc agaatcaact
    14221 gcccgatgaa aaccgcaaat cgagcatgcc ccgtgcccaa tcgtttctcg gtttcaatac
    14281 gtaaggattc aagaacctga aatttctctt catcgaccac ttcacccttc gaaattactt
    14341 tttcgccttg ttgtatcaat ccttttactc tggacaaatt agccagcaat tgtcgttgcg
    14401 atttttccgt gagttcctta tcgaaccgaa tattatgagc caatcccatc tcgagagcct
    14461 gtcggacttt tcttctataa gcagagtcaa ttcccgataa atgtttcact atgtaggcat
    14521 cggcagattg taccgtaaag aaatgttcag gataagcttc ggtggctata ttgcctttga
    14581 caatcatgat ggattggccg gaaggcaggg caataagttc gggaaccatc tgaattattc
    14641 catgtcgaaa gatggtatcg gctatttgaa tagcttttcg ataaacttcg acaataggtt
    14701 tgccggaaag agtatcagaa ggcgacatca ttaaaaaatc gatcagggcc tgagcaacta
    14761 ttactctctg attggcagta acttcaggtt gataggtaaa atacaaacgg ctctttgcca
    14821 atagcctctc tttctgcaac tcatattctt catccgtttt caataccgaa aaatcgaatg
    14881 gagcaatcaa atcttcgtga agccagggtt ttcccttcac atattcatat ttgaactttc
    14941 cttcacgcgg gaaaaataac agaataatta tcaaagcaca taaaaaaagc aagcccttgc
    15001 ttacataata cagatgatcg gtgaacaagc gcaaagggtt tctcatgcaa tttcaaattg
    15061 aatttcaaat tctggtggat aaacttgcat agggacaaga gaatttgaga aatagccaaa
    15121 tcgataatcc tgaaaaatag aagacatttt tcaaggatgg atttgccacg aaaataaaca
    15181 aaatctccta ttcttcggtt ttattttcgc gttttcgttt caaatgatcg agaataatct
    15241 tgcgcattct gatggcattt ggggttactt cgacgtattc atcagctgca atgtattcga
    15301 gggcttcttc gagagaaaag atgcgtggag gtgaaagaat ggccttttca tcacttccgg
    15361 ctgctctaat attggtgagt ttcttcgtat gtatgacatt gaccacaagg tcggtactgc
    15421 gactattttc gccgataatt tgacccgcat agacattttg ccctggttcg atgaaaaaag
    15481 ttccacgatc ttgaagttta tcgagggaat atgtgatagc ctcgcctgtt tccatagcta
    15541 taagaactcc gtttcgtcga tgagggatat ctcctttcca tgtttcgaat cccttgaaac
    15601 gatgggacat caccgcttct ccctcggtag cagtcagcac tgcattgcgc aatcctataa
    15661 ttcctcttga aggtatgcta aattcaagat aagcccgatt attacgtgta caaacccctt
    15721 cgatttctcc cttgcgtgaa gttacgatat cgataatttt acccgtaaac tcttcgggta
    15781 cattaataga aagaaattca atgggttcac atttttctcc attcatttca cggataatta
    15841 ccttaggctg gcctacctga aattcgtacc cttcccttcg catagtttcg atgagtatag
    15901 aaagatgaag cactcctcga ccgtaaacca tataagtgtc gggctgaaat gtttcttcta
    15961 cccttaaggc aagattcttt tgcgtttcga ggaacaatct atcacgaaga tggcgagagg
    16021 taacatattt tccttccttc ccgtaaaacg ggctgttatt gatggcaaag atcatgctca
    16081 tggtcggttc atccaccttg atgggaggga tggcttctgg gttttcgaaa tcagcaatcg
    16141 tattgccaat ttcgaattct tcgggtccta aaacagccac aatatctccg gcttcgacgg
    16201 gagtttttat cctttcctta cctaaaccct cgaaaacata taattctttg atatgtgatt
    16261 tataaacgct gccatcacgg cgaactaccg aaacattctg cctggcctgt attgttcctc
    16321 tgtggatgcg gcctacggca atacgaccca gataactgct ataatccaaa gaagtaatca
    16381 tcatttgcaa actccctttt tctttgtgag gtggagggat atatctgata atagtgtcga
    16441 gcaaatagct tacatcttga gtgggccttt cccagtggtc ggacatccag ccctgcttgg
    16501 ccgaaccaaa cacagttggg aaatccaatt gatcgttcga tgctcccaat gaaaacatca
    16561 aatcgaaaac ggcctcctgc actaactcgg gttcacatcc tggcttatct actttgttga
    16621 ttacaacaac aggtttcaaa ttcatctcaa gcgccttttc gagtacaaag cgcgtttggg
    16681 gcatgggacc ttcgaaggcg tccaccacaa gcagaacgcc atcggccata ttcaacactc
    16741 tttccacttc tcccccaaaa tcggaatggc caggagtatc aataacattg atttttactc
    16801 ctttatatct caccgaaaca tttttggcca atatggtgat gcccctttcc cgttccaatt
    16861 cattggaatc gaggattaat tctccaatct gttgattttc acgaaacaac tttccctgat
    16921 aaagaatgcg atcgactaaa gtggttttgc catgatcgac atgggcaatg atggcaatat
    16981 tgcgtatttc tgtcatgcaa tttatcgaat cgaaaaaagt ttgcaaaatt acggtaaatt
    17041 gcaacaagat atattccgaa atatttcaaa aaagggaaaa ggatggagga atttggatga
    17101 agtaattatc cttcgagttt tacgaagtga taaacgatat atcctttttg ctcgggatcg
    17161 gcattagggt tggaagaaaa tttcgattta agggcggctt cccgtgcagc tttccatagt
    17221 tcctggtcgg ttgtattggt tccgatagaa gtattaggaa cttttttccc aggaatggca
    17281 cggattacat ttccctgacg gtctactgta atttcgacca cgactgttcc ctgctccatt
    17341 gagttgtatg caggctgata gaggaatacc ttggttcgtc cactcaaata aactccaggt
    17401 ccttggcctg tgcctccagt tcctgtatag gaatttgatc cggttgttcc gtcgggtctt
    17461 ccctgatcgc ctattcctcc tgcaatacct tgattggtta cggttccctc cccattcaat
    17521 ttcccagtat ataaggcatt tgggttgagc acgggttctt gtgacttttt ttcaccggtt
    17581 tgttctaaag gagtagtttg cttgttcttt tttatatttt tttctttatg gcttaaagaa
    17641 ggaatttcct catcattggt agttacaatt tcttcatcct ttatcgagcc taaaggtatc
    17701 accgcagctc tttggcctgc aggaagttct gcagcttgga tacttccaaa accctcgtcg
    17761 ctatagccca aattcacctc tacgccttct tcctctggta aaggaagcgg ggtacgcaaa
    17821 gccatagtaa acatcagcaa aaggaataga gcgtggaata ctatggtggc cacaatgcca
    17881 gatactttgt tttttttctc gttcattgaa tttccttaac cggacgagta gccaaaatca
    17941 ccttgtattt gctattgagt tgggtattga tgtcattcac tatatcgaag agtgaaacta
    18001 catattggac aggtacagat tgatcggctc tcaaaaccac cgaagcgtca atatcctgtt
    18061 tttggaggtt ttcggccaaa atttgctgaa gttgtggaag attaactggt acagcatgac
    18121 cacgaccatc gggatcgaca aaatactcga aatgatcatt gatataaacc gtaatggctt
    18181 gcttggccat tgtcttactc ttactggaag gcaggagaag tttaatggca ttgggactta
    18241 ccaaggtaga agaaataacg aagaaaatca ataagaggaa taccaggtct gacatgggag
    18301 ccgggttaaa ggcaatattc agcttatttc ttttttggat ggccatattt taggaattta
    18361 tgcggcgggt tcctgaagga gatccataaa atcggtggct cttgcttcaa gattaaacac
    18421 gagtttttcg attcgagcaa ccagaacatt atagcacaca taagcgagaa ttccaacgat
    18481 aagacctccc accgtggtta ccatagcttc ataaatgcca ctcgagagta caatcaggtc
    18541 gacattgttg cctgccatcg acatgtcgta aaatgctcgt atcattcccg ctacggtacc
    18601 caagaatccc aacataggtg cagctcctgc gatcgttccc aggcctgcta tatttttttc
    18661 gagcctcgaa acctcgagct gtcccacatt ttcaatggag gcattgatat cattgagagg
    18721 tttgcccaag cgcaacaaac ctttttctat catacgagca agaggattgt cctgtgctcg
    18781 acaaagagca acagccgcat ctactctacc attatgaata aaatcgcgga tatggttcat
    18841 gaaattcttg tcttccttcg aagctctcga tattgccata tatctttcca caaaaatata
    18901 cacggcaata attgacatga ttcccagaat caccatgatc catccgcctt taacggctaa
    18961 atcccatacc gaaagcgaaa tttgctgggc ttggggaaca gtgctgccca cattctgtgg
    19021 taattgctgc agtgacttca tcaccggagt atcctgcaaa gctgccaata aaaaaagcat
    19081 attcaatttt tttgcgaatt tacactaatg actcgaattg ctatataaaa acaatagaaa
    19141 tgttgttaac atactcgtgt gaataataac ttaaaccctt gcatattatt gtcattccag
    19201 tcatgcaata ttttttaatt cgaaaactac caagactttc tattcttatt tttccagatt
    19261 aatttctttt cgaggtgcat ttttggctat ggaaaatttc ttctcttctc ctttaagaag
    19321 tcttcggata ttggatagat gagtgaaagg gataaaaata gccactaaaa tactcaggac
    19381 gagaaaaggc cagtgtggtc gaggttgata aggaagaaaa accacaagga atgggaaact
    19441 gattcctgcc agaattgacg aaagggatac gatgcaggaa aggatcataa agatgataaa
    19501 gacaacaacg gcgcccatag taggataagg gaataaagcg aggccgattc ccagtaaagt
    19561 agccacaccc ttgccccctt taaatcttga atataatggg aagatatggc ctaaaacagc
    19621 cgcaatgccg caagcaatca ataagagctc ctgttgcagt tcacctaaag gcttaggcat
    19681 aagccatgcg gctaatttca cagctatcca tcccttcagt gcatccaaaa gcaaaaccgg
    19741 aatgcctgcc ttccatccaa gaacccgcaa agtattggtt gctcctgcat tcatgctgcc
    19801 atatcggcgc acatcaatgc cataaaacca tttcccaatc catactgccg atgaaaaact
    19861 cccaattgaa taagcaataa taattaaaac aatggttatc aacattaaaa aatattaatt
    19921 gaatagcttt accttttcca tttcggctct aaaatcaatg gcttcggctc tattcaccgg
    19981 ggccacacga aagggctgcc aattcttggc cgggttgtcc ttctccattt gtttattgaa
    20041 actttctcgt tctttcacca caaggtcggt aaattcctta ttcgtactgt aaaccctcaa
    20101 cacattacct ctgccataat caatataaaa ataatgatct tcatcgggtc tcagcaatat
    20161 cctcaactga tcttcacggg cattctcctg atgcttaagc tgaatgtatc ccggaatttt
    20221 ccgaccaatg taattcccct tgatatataa caggcttatt ggcccacgat aattaaaaac
    20281 ttgctccacc gaatcccata caaaatgaat atctgtcatc aagattgaat taccgacaac
    20341 cgttgaaatg gtttttccgc tttgcacctc atttatcatt tgtgtgtagc ctttctcgcc
    20401 aaataaagca agaagctgtt ccttatatcg tttatcgtcc gcattcacca agggattatc
    20461 cagagaaaac aattcgttga ctataatttt ccatactttt tcaaagaaag ggaaatcaaa
    20521 agcaaataac agattcatcc taaggctatc ggtttttaaa tcataaatgg cattgccggt
    20581 cgtttcgata cgcgaaacag gactgaagcg aggatggaga tttattagtc catggaccat
    20641 aattttgcat tgttctggat gatattcaaa aagagggtta tccatattga aaatctcact
    20701 gttttgattt ttaacgactt tatacttatt ttcattctgg tcccacaaaa ttttccctga
    20761 agcggtaaaa acggacaagt cacggggatc taataaaggc tcgaggaaac gagcatatac
    20821 tttaccgccc gttcggttca taaaaatacc tgtaaccaat tcaattccag tgggatcaaa
    20881 acatctggcg ggcaagggta aaattacacg atcgggttca acaagtgtat caaatcgaat
    20941 ccatgaagca gacaagccgc atccactttg aaggattcga taatagcctt cgaataaaag
    21001 gggtttggtg tctgatgaaa aatctacctg gccataaaaa tcaaaccatg gatcgagtat
    21061 aaaggatgaa gtatcattaa cggtagcact accgtgtgta atgctcgtaa aagggtcagg
    21121 ttttatttga tcgaagaaaa tcaaactcgt tttttcttga cgatccagtg agaaataagt
    21181 tgttccttta gcttggaact ttttcccgga gataatttct ccgtttacgt gagaaaattc
    21241 acgtcgtatg tcgttacgag aagtaataag gcgagcattt ttaaaagaga caggaaggtt
    21301 ttgcggggta atctgaattt gtttttctga gaggaatata taagcatcag ctgattttat
    21361 gaaagggatg tcagtaaagt tcaacaggtt attctggatt tcataagaac ccgaaaacga
    21421 aataaactgc agagaatcca tattgggctg caaggcaacg aatgtaggcc ctttaagtgt
    21481 attttcttcg aaagcggtta tcaattcatt tggtttttga aatgccatgt attgtttttg
    21541 agaatcgaaa tcaggagatt gaagttgtat cttcttttga ttcatatccc acagaaactg
    21601 gctgtgatta gaatagcaag aaaaaaccgg gaaatctatc cgtgcgatgc gtcctgcatt
    21661 aaaaacagcc cgggcactgt cgaaatccac aaatgcgttg taattgacag ctcgaatggc
    21721 aacagctttt tcgtcagggg tatttagcaa aaattgggag ctatcggctt ttaaggttct
    21781 tcgtctaaac tgataaaaat ccgaatttaa gcttgccttg tcgaagccag ctttcccttg
    21841 tccatcgaga ccatcgggtt ttagcataag gtttccttca aaagccatca tgccgttata
    21901 catcgaaaaa ggattcttaa tgcttttcag gttcatcaag tccttatagg gataccagtc
    21961 ttcctttatt cgtcttcctt ttaccgaagg aatttcgatg tcttcggaag attcggttat
    22021 ggagaatgac aacacagatg cttgagctct ttcggggaaa aagacgaagt ccgtcgaatc
    22081 ggttcgattt gtagtatcgg taattgaaat gctttggagg tagttaagct ggccatcgcc
    22141 gcgaaatccc tgatggctaa ggtcgaggcg gcctgcaaac catcctttgg gctggccgcc
    22201 atgcaagtaa atagcctcac ccgatttacc tgtatgtata tctattccaa ggctgtaatc
    22261 aggtcgaatg attaaaggtt cttccataac cttgaaaata ttggccgagt ataaggtacc
    22321 tttcaaattc aggctatcca gataataatt atcgaggttg ttgagcaaaa agggttttac
    22381 tttataatag aaacttttgt gaggatattg ggttttgtat ctccccttgc gatcgtaaaa
    22441 tacatacgaa ggtgtggaat cggttttaaa aaccggataa gggatcttaa ggtttttacg
    22501 ccctgatttg ttttgaggat gatctatctg caaggtgccc gatacctgtt cgatttgaga
    22561 ttctatcttc actagtttcg ggttgatttt tccggtttta gatgatttag gaatatcgat
    22621 agccatgaac tggatagaat caatttcggg cagtcgtagc tggaattcct catataagaa
    22681 taaatttcgt cggttgctgt aaaaatcgaa ataaccggct tttgttctgc cgctaaatgc
    22741 gaaattacgg ttctctttca gagtgatcgt ataattatag ggagtaacca ttactttttg
    22801 agaatcggaa agtatcacct gatcgacact aaaaacctgc aaatcgagat tgtggagatt
    22861 caatattgcg ttggtatcgg ttttactcga taggatgaca aggttgtcga aatcaatatc
    22921 cccataataa gaatcgaccc aatgaaaaag tttttgacgg agtataattt ctttttgttc
    22981 aaagtcgtag agtaaaaatc ctctggcggc catttgaaga gcgagacgct cggcttgatc
    23041 ggccgaaaga tgaatggcac ttgcatactc attcaatccg aattgtgatt tatgaagttg
    23101 tttagcaagt tgatacagac gtatgagagg gtttacgggg tctatgccct gaagggttct
    23161 aaaatcttct cccctgaaat aattttctga gataaaaacg gcatcatttt ctcgcacaag
    23221 tccggctcct tttttaaaaa tcatgagcga atcatcgata ttccataaaa gtgtattagc
    23281 ctgaatttcc attctttgat aggagctaaa aaagggcaaa gatgatagaa tgctattgcc
    23341 ttgataaaga ttcaattgtc gtttgttctc gtcataaaca aatgaaatgg cgggatgaaa
    23401 gatagaatcg aaccgatatc gaatagtcgc attggcgtcg agagccgata taaaaggatt
    23461 tttgaaaaag aattgctgag attttatgac gactctattt ttctgtggag tataaaaaaa
    23521 aagccgggct ggtcttccgg attcaccaat tccgttaagc tggtgggtct ctaattttac
    23581 ccccccctca aaatcgacat tctgagaaat tccggggatt ttaatattcc gatcataaga
    23641 tacaaaacgc ggcaaaccag gtttattacc aggtttgaaa ttatcttcga atttccccat
    23701 tactcccgaa gggaaatagg ttttatgata caacatcgcc gaatcggcct ggatatcagt
    23761 cccttgtaga aatattccat atcggtcgaa ctcaacataa acagaatcag atggtatatt
    23821 gttatttacc cagggagttt gtccacccac acctttccat tcgcgtgaaa agggatcata
    23881 aatgcctaaa gtctggtaaa ttttacgccg tgaagaaccc atctggcaaa tcaggtcgat
    23941 gtctttaaat ttgaaacgag gtgttggata attaatgatt tcgaatcgta tttgactgac
    24001 tttccaattg atgttcttat agcggctaag caagctatct cgaagcaagg aattggaaat
    24061 gtgataaaaa tcgttcaatt gagaaggacc gggctgatca agaagataag aaactccatc
    24121 aaggaatggt tttacctgcc cctgctcagc attgagaatg gcaagattta cgatagtcgt
    24181 aagaaaagga gcaatatgtt cataaggctt caagcgtcgt tccaacatct tttcgaccag
    24241 agaattcact tgtgggatca tttcatcgag ttcgtaatgt gattgccaat ttgcctttaa
    24301 ctcgtttaca aatacctgac ccgaaaaaat tacctcttta tctttgtttt tcagttcgat
    24361 cttcgaaaaa agcgcatcca ctcgcaacca gaaggcttcc tgttcattta gttgaggaaa
    24421 cagacaaagt gattggaaga acaataacgt aaccaggaaa actattcttt tcatcttatt
    24481 aatctaaggc ttacaattca ttcaaacaaa tttacatgat aatttccaat cggcaccccg
    24541 acggataaaa attcgctttt atcgagaatg gggaagagtg agtaaaaagt taaaagttgt
    24601 caatgcttga ataacaatga cggtttttga gccaattatg ggtaaagcga gaagaaaatg
    24661 ccacttgcca tcttctctaa aagctgtaag ctgatagcca ataaacaatg atccatcttc
    24721 ggattcatgg gaagaaataa tcatttgcct taatatgatc cataaggatg agagattcga
    24781 ggataaaatt ggtgaaggac ttcttttttt ataggttttt cgatacgaaa gataagtttc
    24841 caatcattaa ggcagagttt tcaaataaac agcagccagc caactgtttc tttcttttat
    24901 tcggcaagat gcgaggccta ctcggtgaaa agcatcatct ataactggta gatcatcttt
    24961 gtaaaaaccg cttacgatca atagaccgtt ctgtttcaaa accttcatgt aagcgggaat
    25021 atcattcaaa agaatattac ggttaatatt ggccaaaatg acgtcaaatt cattcatagt
    25081 cgaaagcaaa gatgcatctc cttgttttac gacgattcga ggtgtattgt tgagttgaat
    25141 attttcgaga gcatttcggt aggcccattc attgttatca atagcaacga tattgacggc
    25201 tcctatcttt gccgaaagaa tggccaggat tccagtaccg gttcccacat ccagaatgct
    25261 tttccccatc acatcgattt cgagcaggta ttccatcatc atggcggtgg tttcatgatg
    25321 ggctgtacca aacgacattt tgggttcgat gatcagttcg atgggataat gaggctgagg
    25381 aaagtgaaaa ggagctctta caaagcattt ctcagcgata acgaccggtt cgaattggct
    25441 ttcccaaaca gcattccagt tttgttcggg aacagtccga aaggtaaaga aaggagcaat
    25501 atccagtctc tgcaataatt cttctacggc atggcggtca aaatgaactg aagggatata
    25561 tcccagcaaa gaatcttctt cttcgataaa accttcaaat cccagctcag ttaaaatgga
    25621 agggagtaat tctttggttt cgtcgtcaat aattataggg attcgcagct cagtatattc
    25681 catttttaat caaaggattg ccttgacaat ctcgataaat tcatcggcat taaggcttgc
    25741 tccgccaatc aaaccgccat ccacatcggg ttgggcgaaa atttccggag catttttcga
    25801 attgcaactt ccaccataca atatggaggt ttcgtcagct acatctttac catacctcga
    25861 tgcgatgagg ttgcgaatgt aagcatgcat ttcctgagcc tgttcaggtg tggcattcac
    25921 tccagtgcca atagcccaga cgggttcata ggcaatgacg atgttgtaaa actctttttc
    25981 ggacaaagga aagacggctt tctcgatttg ttcacgaact acatcgaaat gtttcccggc
    26041 ctgacgttca cttaaaagtt cgccacagca gaatatagtg ataaactcat gttcgatggc
    26101 ctgatcgatt ttttttgcaa tgatttcgtc ggtttcgtta aaatattttc ttcgttccga
    26161 atggccaaca atacaataat caacatccat cgaagccagc atacgggccg aaacttcccc
    26221 agtataggct ccattgtcga aatgactgat gttttgagct cctaccgaaa aaacagattc
    26281 tatggctata tcgagggcca tctcgagata agggaatggg ggacaaatta caacctcggc
    26341 atgcccaagg tcgatatttt caagctcatc ggctatggcg gaaataagat cttcggcttc
    26401 ctcgaagtct aaattcattt tccagttacc ggctacaatt tttagtctca tgataaaaca
    26461 attttttaaa ttatatattc agaatgaatt aaaacaaaat caaacgtgcg aaaataataa
    26521 atataactgt tcactgccgg gagcaaagta aaattctaaa atctattggt aaacgaatag
    26581 ccgatcgaat ctaccataaa ataagaaacg cgccattcgc tcggatagtt aatttggaaa
    26641 atctattgtc attttatgtt tccaagtaaa tcatcattcc agaattgtga tatggaacct
    26701 gaacaatgag cctgtccgaa ttcgtaactt cagtttcaaa gtccatcaaa tcatcctctt
    26761 tatttcataa caaaaatgga atatttccat cctgtaactt ttccacagga caattacctt
    26821 cgattacagg atgaataggg gaattcgaaa aaaatagtta tagtaaaggc attgaaaggc
    26881 aaaaggaaca tgagaagtaa ttccttcagt tactccagat aatatcgccc gagccctgtt
    26941 tttgaatgtt acgattattg ggattccctt ttattctgat atctcccgag ccatggattg
    27001 agccagtaat ggattgtatc acccaaaggc tcacatcacc tgagccataa cagtgtattt
    27061 ctgctttatt gcaaggcagt tcaaaacagt ggatatcacc cgatgaatta gaataaatat
    27121 ttacttcatc ggctttaccg ctaagtttta tatcgcccga cccccgattg gtaattttca
    27181 ggttcgaaag ttgaaaattc tttcctttca tatcgcccga acctgctaca tggatctcgc
    27241 acgagccatt caccccttcc accaataaat cgcccgaacc attcaaataa gattcaaatt
    27301 ctttgcattt gagctcgagc tcggcatctc cacttccatt gagtgttata ctgaaacgag
    27361 gaaaattcaa ttgattcatg ctcaccacat cacccgagcc attgattcta atcacattca
    27421 aattaggcac agtaataatt gcacgcatca cctttatctt tctcaacccg ggcctttttt
    27481 ttacatcaat gtaaagtgtg gaatttcttt cttcggtatc gatataagaa atgatttcgg
    27541 agtcggcttc aatcttgact tcgaaactat tgccctgtaa aagaagaata tctaccgatg
    27601 aattgttttg aatggtttga aatgaaccca cctggcgtat ttccgaaatt acattcgatt
    27661 gcccaaaagc aggaattatt accataataa aacctaaggc cgccaccaac gaaaaaactt
    27721 tttttctcat agcttttaat ttatccagtt taaacaaaat tacgactcca taaacctgca
    27781 aaagttacac atgagacaaa atattttaca atttgcccag atcgaaatca ttacttttgt
    27841 acaaaattag agttatggct ctgcgacatt ctttcgagaa aacaggtaat ttctttttcc
    27901 gataccgtgg gcaaataccc gtaattattt ttatttcgtt aattccggcg atgttcttta
    27961 ccgattatag taacctcgat ccttcaattg ccaaatgttt gaccgcaatt tcaattgttt
    28021 tagctgcaac cgggttttta gtcagaataa taactattgg cacaactccg gcaggaactt
    28081 cgggtagaaa tactaaatcg caagtagccg aaaaattgaa tacctcaggc atttattcca
    28141 tcgtacgcca tcccttatat ctcggaaact atctcatatg ggccggactc accttgttcg
    28201 ttttcaattt ctggtatttt ctggttattt cgctcgtttt ctggatttat tacgaaagaa
    28261 ttatgtttgc cgaagaatgt tacctcgaac gcaagtatga ccaagaatat attgaatgga
    28321 cattgaaagt tcctaccttt ttcccctcct tcaaaaatta tgatccttct gcattaccct
    28381 tctctttcaa atcggtatta cggagagaat attcgggtat gctatccatt gtgtttggtt
    28441 ttgccttcat cgattacctg cgctattatc tgttgaatag aaatttccta tctgtcagac
    28501 cttcattgta tatcctggtt gctgccctca tcattgcgct ggttcttcga accataaaac
    28561 accacacaaa atggctggaa gaatctggtc gaacataaaa gatgagttcc accatagaaa
    28621 aataaattta ggagggttgt ggaaaaatga acgaaatatg cctactgcct gcaaaagctc
    28681 gagcgagagt aaatactgta aatatttata aaaggcaaaa gacaaatatc ttacccctaa
    28741 actttggttt tctaattacc attggcagga ttggaaacaa gaaaaaatga aatgctttct
    28801 gttataaatg ttttcccgaa tcagttttgt aaaatctttt tgttatgaaa tacccgaata
    28861 atttcagaac tcctgattcg acactaaatc aagttgtcgt ggttttgaag attgcgatat
    28921 agcatcacct attctcaatg attactttca tcacgtggta aaggcgcact cccttcatcc
    28981 gaaacccaca attttttaag tttcctttcc tgacctggta atatctatca gactcactta
    29041 agccaagtta ttttaatgat aaaacctaac aaatatctaa aacctgttag gtttacacga
    29101 aaatacaaca atttgaaatg cgatgctgct tttgtcccgt cataactaag gggattgaaa
    29161 tgcaattata ggataaagaa ttctccattt tatttatcaa aaaggcaggg aatggcgatg
    29221 tttttaaaag aaagtatatt catggaccgc gacctgctcg aaaatattct taaactcgat
    29281 aacgatgaaa ttgaattgca actggaagaa tatggagaat tagaagggag attacccaag
    29341 aaacacaaac gttacaatat tgaaagcttt atataaaaag aacgattcat caacgacatt
    29401 aaatcggatt aactcttttc gagaaaataa tcgaagaact cgagaaacaa aaactggttg
    29461 gaaacgaccc caaggcactg aaactctatg aatgcatcag agcaaaaatc ttaaaaaacc
    29521 tttgaaagag caacctgtta gcaatggaat ttactctgtg agttcaaact cctccttcga
    29581 gtcggttgaa gattttctga atatccgatt caatctcttt acgcagcttt atctctcggg
    29641 ctatttgctg tttaagttcc tcattctcat tctgccaacg aatgatttca gcattcaaag
    29701 catcgatttg ctggggcaaa ggcgaaagat tcacaagctg ttcttcgaga ctgatttttt
    29761 cggtttcaac actttgcaat tgagccttgc ttctttcgag ttcatcgcac atcgaagata
    29821 ggctttccat aacaaagttg tgctccgaaa caagaatgtc cttttcttca agaatatttt
    29881 tcaacctttc ttcaatttcc ttgatcttgt cttccaattg taattgaatc tgctgggcgt
    29941 tctgtagttc catcttatac ttctgttctc tttcttccaa agattttcgc aaaagttcat
    30001 cggaactttc agtgactttc agtaattctg attttaattg ctcgatattc tcctgaagct
    30061 ccaagatttg ttgctttaat tgaatatttt ccgactttag gagattgttt tcggtaataa
    30121 tttcatttat agtcgattcg tcaggcagat tctggtactt ttcagccttt tccttcaatc
    30181 gcttcatctc gttctgcatc tcttgcttct cgttcgatat tgtttctaaa caatccttca
    30241 attggttatt attttgtttt tcagaaatta aagccctgtt cagttcggtt atctgttgaa
    30301 ggagttgttc cctttcatta taaatccgag ccagttcgtc gttgatttcc tcgagcttat
    30361 tttcatattc gagctgtgta ttttggaaag tttcgagttc ttctttggtt tttagtccgg
    30421 atttgttggc tttttgaagt tttctaagca agataagtgc aaaaattcca agcaagatca
    30481 ggatgaggga tacacccaaa agaatgacat aatcacgggg gctcaggtcg ttttcgggtg
    30541 atgaactctg tttcatgtat tcaattcttc ccttgaggtg agtaatggta tcgagcagtt
    30601 gctgttccct aactgaagga atgcccgcag ccaattgctc aagacaagcg gcaattaaaa
    30661 tgctgtcgga ttggatcact ctggcagcaa ggccattgag catcaccact ttttcccatg
    30721 ttcgcacctt gatgctgtcg cgttgctgaa tgtaattcga atagtctttc tggcgagttc
    30781 taagcaactg attgataatg ctatcctgtg cctggctata aaaaaatgag cccagaaaaa
    30841 gaaagggaag aatgagaatt ttctttgtca tatttatgaa ggaataatgg gatttttaat
    30901 gagtttaacc aatgcttctt cacaaaagta atatttttgt ggtgtattta aggtaaaatt
    30961 acattttaat tttattcatt aaggctcagt ctatgagaaa tgtactattc attcttgcag
    31021 tgtctctatt ttccgtgttg agcgaggtga atgcccaagg ttttcaactc aaggttaaaa
    31081 taaagggtgc caatgatgat ttcctctatc ttgctcatta ttttgccgat aagcaataca
    31141 tggatgatac gcttcgaaaa ggaaagggag agtggtttgt tctggaagtc gattcaatgc
    31201 ttacgggggg gttatatatt atagccggag aaaaaaagaa ccgatatttt gagttcctta
    31261 tgaataatga gcctcgtata agtttcgaaa ctattgcaga tgatcctgta aacaacatgc
    31321 aaataaaagg atcatcagag aaccttgcat tttttcgata cattcattat ttagggaaga
    31381 aacaaaaaga gatcgaacct tatcagaaga ttttcaagcg gaccgaaggt ttgtatcccg
    31441 attctgccag tttggccaaa gcttggatgc aaaagatcaa tgatgaagtg atgtcttata
    31501 tcaaggaatt tatagaatca catgaaggaa tgtttgcagc tgatttcgtg aaagcttcgc
    31561 aggagccaga aatccctgat cctcctttac ttcctaacgg aaggcccgat tctctgtttg
    31621 cttataaata ttacaaggcg cattatttcg ataacctgcc tctgaatgat gcaagattac
    31681 tgagaacccc cttctatcat caaagagtga atacgtattt tacgaagatg gtattacaac
    31741 accctgatag catcattgcc gaggcgaagc gtttaatccc catagccagt caatcagccg
    31801 acacctaccg ttatatgata tggttcttga ccaattttac cgaacgcagc gaaattatgg
    31861 gcatggatgc ggcttttgtg tatatggtgg aaaattatta tgcatcgggt gaaatggatt
    31921 actggatcaa taatactgta aaagaaaatt taataaaaaa agccaaaaaa cttaagccaa
    31981 ttttaattgg ccagactgct cctgaactca tcatgctcga tacctttatg caacccattt
    32041 ctcttcatca acttaaagcg aggtatacca tacttttatt ctgggacccc acttgtggcc
    32101 attgtaaaaa agaaattcca atgttaaagg atttcatgac aagaaagggt aaacaatacg
    32161 acatagaagt attcgccgtt tgcagtgata ctaatatgcg cgaaatgaaa aattatatcc
    32221 gtcagtacga tatggattgg attaatgtaa atgggccaag agcctataca gccaactatc
    32281 atgatctata cgatatttac tcgactccta taatatatct gctcgacgag aaaaaaacca
    32341 ttttagcaaa acgtctaact gttgaacaaa tcgagtcatt catagaacat cacagccgtt
    32401 ccgagagcgg tggcgtgctt caaatgaagc aggaatacag gaataataaa taaacatgta
    32461 aacataaaaa cagaaactcg tgttataagc catacctcca gcatatcacc tgccttcaac
    32521 ttttaacttt ttaactcacc attcaccatc ctgcaaactg aagcat
//
LOCUS       DAOORU010000011        29795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_1790_length_29795_cov_5.382078, whole genome shotgun sequence.
ACCESSION   DAOORU010000011 DAOORU010000000
VERSION     DAOORU010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 29795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29795
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1790_length_29795_cov_5.382078"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(122..952)
                     /gene="rhaD"
                     /locus_tag="PLF20_01735"
     CDS             complement(122..952)
                     /gene="rhaD"
                     /locus_tag="PLF20_01735"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: protein motif:HMM:NF002963.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HOX79766.1"
                     /translation="MKISIPELKPLLKEIAEVAYNVWAHGWAERNAGNISMNVTPHIR
                     TMDYISDKKQEQHRLPFLFPNLSEQILLMSATGSRMRELAEFPHKYMMLLRISSDGDS
                     YSIIPLANHSFHLQPSSELLAHLAIHQQLIEEDSLNNTLLHTHITEVIALNHFPNLNE
                     KNALNKVLLSMHPETILFLPEGAGFVPFLPPGSQELARKTLDEIKHHSVVIWEKHGVL
                     SYGTSPANACDQLELIAKAARIYLMCRQASVSPSGLSIQQLRQLKEKNDLPPHLPFTS
                     "
     gene            complement(949..1881)
                     /locus_tag="PLF20_01740"
     CDS             complement(949..1881)
                     /locus_tag="PLF20_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOX79767.1"
                     /translation="MNRIFTIGETVMDIIFRDQHLETAMVGGAMLNTAVSLGRLHMPA
                     FLISEWGNDGVGEITQNFLTNNAVNTSFCKSYHGNTSLSVALIDTKGNASYTFYKNYP
                     EKRFQIDTPDFEPGDFVLFGSFFSIDPGIRTQMTNILKQARNSGALLIYDPNFRKNHL
                     ASLEQLLPFIEENIRYSHIIRGSHEDFHYIYRVDSCQKLFEKVHSLGCEVLIITHGSD
                     EILLFKECTKTRVAAAAVEAVSTIGAGDSFNAGLMYELYRRQISPNNLNMIPSCEWAE
                     IVSVASSFAANTCSHYENYISQEFAQHILSSQAI"
     gene            complement(1997..2692)
                     /locus_tag="PLF20_01745"
     CDS             complement(1997..2692)
                     /locus_tag="PLF20_01745"
                     /inference="COORDINATES: protein motif:HMM:NF040157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HOX79768.1"
                     /translation="MPEESCRICGNQILETKEFHSVRGYYCKQDNQLKKTCKQFHYIC
                     FGSTNQLINEMIFTLCMDTGLSDPDEIDLQAFDHLGIPVHGPQHHFLISMALTVAYLN
                     SIHHNYKKEEHLKEKRERSERIPGGFCWTYDTCGAAIIVGMFISVIIRAQWFSEKEWQ
                     LSNMAITRTLKSIAKVRRPRCCKRVTFISSRNAQNFLEKLLQGKLSVHVYIVCHPHDL
                     NKECFQNRCMFHF"
     gene            complement(2941..4212)
                     /gene="rodA"
                     /locus_tag="PLF20_01750"
     CDS             complement(2941..4212)
                     /gene="rodA"
                     /locus_tag="PLF20_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOX79769.1"
                     /translation="MNTTRYKYHNPFAGIDRTLLWLYFTLVIFGWLNIYAAVYDEIHK
                     NIFDLQTNYGRQMIWIATSFVIAMAILLTDVKLFNSFAFFIYGLVIVSLILVLFVGKE
                     ISGSKSWLQIGPISIQPGEFAKFATNLALASYLGNIKRNFSKAGVKIAAFSIILIPAL
                     IILAQNETGSALVFLSFILVLYREGLSAKVLIAGAIIIALFVLALVLDKYILIGLCAL
                     ILLVYYLTRRNRKKLHWILIGIFIATSSITFSVDYVFHNVLQPHQRSRINVLLGKETD
                     IKGAGYNVYQSMIAIGSGGFWGKGYLKGTQTKYNYVPKQSTDFIFCTIGEEWGFVGSL
                     ILIGLYVGLMLRILQLAERQRSAFSRIYGYGVASIIFAHFAINIGMTIGLLPVIGIPL
                     PFVSYGGSSLLAFTILLFIFIKLDAHRHDII"
     gene            complement(4209..6011)
                     /gene="mrdA"
                     /locus_tag="PLF20_01755"
     CDS             complement(4209..6011)
                     /gene="mrdA"
                     /locus_tag="PLF20_01755"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOX79770.1"
                     /translation="MSQLSSHSQKRYVVSGIIIAVAAIFILKLFYMQVISDKYKILSE
                     SNVRRIVTIYPARGLIYDRNGRKIVSNEEAYDVMVVPRQVRNIDTAELCQLLEIDMQS
                     FTNRLNKARQYSPYRASPFVEQISKTTYGYLQEKLYRFPGFFVQPRTLRTYPYPIAAH
                     TLGYVGEVNQKEIESDPYYRMGDYIGKSGIEKSYEKELRGVKGEKIVLVDVFNREKGS
                     FENGLYDKPQMAGQDLITSLDLDLQLLGEKLMMNKRGSIVAIEPSTGEILAIVSSPSY
                     DPNLLVGRVRGENFKRLSQDPLNPLFNRSIQATYSPGSTFKLVNALVGLQTGAITPES
                     RFYCNGKASLPIACSHSHYSPLELIGAIEQSCNPYFWGVFKTTIEKTGGGDIHKGYNI
                     WRQNVMSLGFGNYFDTDIPNQVKGNIPSSQYYDRYFGEKGWKALTIRSLAIGQGEILI
                     TPLQLANQAVVIANKGYYYPPHVVKSVQGKPTRWNKKQSSIDSQHFETITKGMYRVFQ
                     GVSGTGRWYKLENIDICGKTGTVQNPHGKDHSLCIAFAPAENPKIAISVVIENAGFGA
                     TWAVPIASLMIEKYLTGKISRTELEERMSQANLL"
     gene            6387..8486
                     /gene="recG"
                     /locus_tag="PLF20_01760"
     CDS             6387..8486
                     /gene="recG"
                     /locus_tag="PLF20_01760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOX79771.1"
                     /translation="MKLIETPIEYLKGVGPVRGDLLRKELQIFTFEDLLYYFPFRYED
                     RTIIYRIKSLNPSMNYVQVIGKVEMMETLGSGRNLRLHVYINDGTGRLELVWFQGLKW
                     AKQLFKSDLTYLVYGKLNYFNGTYSIPHPEVEIYDPSNGDDIKALYPVYSSTEKLKSR
                     GLGSKGISKLVKTLLLTLEGQIQESLDEMVIDEMNLISLSKALQFIHFPPDQFSLESA
                     RLRLKFEELFYAQLSVLQLKHQRKSFSHGHTFQKVGKFFNEFYEHHLPFALTNAQKRV
                     IREIRADLRGPRQMNRLLQGDVGSGKTLVALMVMLIALDNNYQTCLMAPTEILAQQHY
                     NTFLEMLSGMEVEVRLLTGSTRQAERQEILNGLLENRVQILLGTHALIEDQVQFANLG
                     LVVIDEQHRFGVEQRAKLWIKNNIPPHVLVMTATPIPRTLAMTVYGDLDYSVIDEMPP
                     GRKPVKTILFTDSDRLMVFSFMKRQIAKGSQVYVVYPMINDSDKLELKFLEDGYISVT
                     REFPLPEYAVSKVHGQMKPQDKEYEMQRFQKGETQIMVSTSVIEVGVDVPNATVMVIE
                     NAERFGLSQLHQLRGRVGRGDNESYCILMASDKITSDARARLDTMVNIHDGFKIADVD
                     LILRGPGDLNGTRQSGIMKFRLADLIEDKDLLDKARNLADKILSYDPLLTQPQHAIVA
                     DQLKNKRKDLLPLSMIS"
     gene            8552..11014
                     /gene="pheT"
                     /locus_tag="PLF20_01765"
     CDS             8552..11014
                     /gene="pheT"
                     /locus_tag="PLF20_01765"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOX79772.1"
                     /translation="MKISYKWLQRYIDTPLSYQEISEILTESGLEVEAIETIEFIKGG
                     LKGVVVGQVVSCQKHPDSDHLHLTKVDIGNQEPLSVVCGAPNIAEGQKVLLAKVGTTL
                     YFKNEPMAIKKAKIRGVVSEGMICAEDELGLGTSHEGILVLPPDAPIGMPAAQYFHLK
                     EDVVFEIGLTPNRTDATSHIGVARDLHASIIARRPEIPSHFQKPSVENFKIDNHDFEI
                     EVEIADAQACIRYSGLTLTNVRVGESPGWLKELLQSVGIRPINNVVDITNFVLMETGQ
                     PLHAFDANKIKGRRVIVKTLPEGTPFTTLDGIERRLDSTDLMICNAEEGMCIAGVFGG
                     LESGISASTQSVFLESACFNPSYIRRTSRRHQLFTDASFRYERGSDFNITIYALKRAA
                     ILLQEIAGANVSSEIKDAYPSEIEPVKVDLRYSQVKRLVGQFIPKRRILSILQNLEYQ
                     ILRESPQSLLISVPSCRVDVTREADVIEDILRINGYNRIAFREELHTSLSYFSHPGPE
                     QVKWEIAGLLAGNGFYEVINNSLSFSQHYTLWPEDQRRTLIKLFNPLSRELNVMRGDM
                     LPGLLENVAFNLNHQIDDLKIFEFGKIYRFDGNLDPNADVSVRYTEPQQLAIAVTGLR
                     ENESWEKDHRRADFYYLHSILRKVLQRVGVNLRLLKIVESQIPFYDQGIELFFGNEPL
                     AWVGIVAKAMLQKFDIRQEVVAAVVNWDKVSSFPREMNIEFEEIPRFPEVRRDLALLL
                     DNHIKFAEIEALAWKTENRLLRQINLFDIYVGENIGPGKKSYAISFILQDKEKTLTDE
                     DIDKTMAKLITAFEQKLGAQLR"
     gene            11214..12842
                     /locus_tag="PLF20_01770"
     CDS             11214..12842
                     /locus_tag="PLF20_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HOX79773.1"
                     /translation="MNNNKTLVYSVSPSGEEFPLPQNDDYIAERKRIESLVGEARSER
                     KEIVVVMGLGFVGAVMAAIVADTRNSMGEYSKFVIGCQRPSKRSYWKIPILNTGVSPI
                     KADDPEVDELIHRTVVEKQTLTATFNDDCLEFADVVVVDVQCDFTKFELGNMRSGTAD
                     MAALEATMKTIGKKIPPQCLVLIETTVAPGTTEFVAWPILKKAFARRGIESQPLLSHS
                     FERVMPGKEYVASIRDFWRVASGCNLEARQRVEKFLREVVNTEKFPLTIMDRPIESET
                     TKIVENSYRATILAYLNEWSLFAERNGVDLIKVIDAIKMRPTHSNIIFPGPGIGGYCL
                     PKDGGLGYWAYKHILGFDDADKLFRITPEAIDINDTRSLHVAELTRDALRNMGRYIAG
                     SDVLVCGASYRQDVGDTRYSGSEIIVRKLTEMGADIRVHDPFVDVWYEFVNQDENAHS
                     WKRFFHYQDGLKNLQILKDLPKAMEGVEAVVLAVPHAPDDAVKWAGGKIAVIDCFGIL
                     PDDKIRRYFQLGCEVKALGRGHIQRIKREVRGLE"
     gene            13167..13877
                     /locus_tag="PLF20_01775"
     CDS             13167..13877
                     /locus_tag="PLF20_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX79774.1"
                     /translation="MITIVLASGIAIAGQYETDTFETKNHHKLTINFIKHGSLMINFD
                     DFIIQVDPVSMFANYSTFPKADLILITHEHDDHLDTEAIKALMKPNTLLIANEASLKR
                     LGKLKTKVMKNGDRLDVSNQISIEAIPAYNTTKGHEHFHPSHRDNGYILTIDGLKIYI
                     AGDTEDIPEMKNLKNIDIAFLPVNQPYTMTVAQAINAAKIISPKVLYPYHFGNTNVKQ
                     IQEGLKNSGIDVRLRKME"
     gene            complement(13980..14657)
                     /locus_tag="PLF20_01780"
     CDS             complement(13980..14657)
                     /locus_tag="PLF20_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOX79775.1"
                     /translation="MKNECIDCPCIVKYFRTLNKCQAEELGNNHVVVRFKKGYPIIKQ
                     GTFSTNVLFLRKGLVKIHITGPYREQIVRLIKRPSYLGLPTTIGDKINQYSITALLDC
                     EVCFIDLNIFNRMLEQNKDFSNYILLELCRSEVESFRHCANRTQKQLRGNLADVLIDF
                     SENIFESNKFLLPLSQSEIGNLVDTTRESVCRVLSEFMSDGIIRMEGKKIEILNTRSL
                     KMISQNG"
     gene            14785..15351
                     /locus_tag="PLF20_01785"
     CDS             14785..15351
                     /locus_tag="PLF20_01785"
                     /inference="COORDINATES: protein motif:HMM:NF025066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HOX79776.1"
                     /translation="MESIDQTTIEKLKAEIEALKSTSKRLESSRKDQLSIAIISGDMD
                     KIMAAMIIALAAAAMDTKVKLFFSFWAIGALRDPAKHPKGKNFISKMFGWMLPKGVDH
                     LKLSKFNMCGMGPWIMKKLMNQQNVLSLREMLKEADELGVEITVCEMTMNLMGFKKEE
                     FIDYPHLRFAGAATFVAEAGESSMQWMI"
     gene            15468..15719
                     /locus_tag="PLF20_01790"
     CDS             15468..15719
                     /locus_tag="PLF20_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOX79777.1"
                     /translation="MTKEELKNLKVAKSVDARGTACPGPLLAAKKAIGDIESGEVLEI
                     LSADEGTKNDIPRWCEKVGHQFLGYFDEDSYSRLFLIKG"
     gene            15809..16180
                     /locus_tag="PLF20_01795"
     CDS             15809..16180
                     /locus_tag="PLF20_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HOX79778.1"
                     /translation="MVFSTDKISDPGIDQAGLRKIHYSPSVYVISLPCSSGVKPKWIM
                     HALENGFDGVFIAADGHECSFSSRCAEYTNKIVEVSQQLMKAKNINPKRIRMAALCSV
                     CAEPFANHMENFGKILANLED"
     gene            16318..19116
                     /locus_tag="PLF20_01800"
     CDS             16318..19116
                     /locus_tag="PLF20_01800"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX79779.1"
                     /translation="MDKQESKYDALVVGGGIAGQEMALSLADMGYQVLLVEKGLSIGG
                     KMIQLSKVFPTLDCAACITTPKMSETSHHPNIRLLLNSEVEEIKKNGTEFSIRMIQKP
                     RFVIQENCTGCGECEAICPEIRNDDYNANLAGRKVAYIPFSLANPRIAAIDRQDHSAP
                     CINECPGGVKAYGYITLARNGQYEEAMKLHLEDIPFPGSLGRACYAPCQNTCTRGKLE
                     GPVDIRKIKRFFSDWYYEKYPEPLAVEIREKSGKRIAVIGSGPAGMTAAYHLALKGHT
                     VKIFEAAPQPGGMLRLGLPEYRAPKHIVDRDLKNITVLGVEIEVNKEVADIKALKEQG
                     FDAVFIAVGTHEAFTSKIEGSELKGVFDCLKFLREANIGKKDDLTGKKVMVIGGGNTA
                     IDAARTSLRLGAEKVTIVYRRSREEMPCFAPEIQEAEEEGVEILILSNPVKYIGENGW
                     LKQVQLIKMRLGEKDSSGRRSPDPIPGSEYIRDVDYVIEAIGMRPHTKPFAGQIELNK
                     NGTIKVDEKTLQTSVEYVFAGGDTVMGATTLIEAAGQGKKAAFYMDKYLRGLNMCDFE
                     YGDKLPAVDKSSILRRYKGSVKPPILGEMLSSNHRIKNWDEIEKGYTETELKASTDRC
                     LDCGNCRECHQCISACPANAIDFSQKTQIVDITAKSVILTTGYKLFPPNGKPNYGYTK
                     YANVIDSLQMDRLIAPTRPFNNVLRPGDGKIPDNIAYILCTGSRDSAIENSVCGDECS
                     NNPICSQICCMYSIKQAQLLMGALPMADVTVYYMDIRAFGKGYEEFFQQTKSMGVNFI
                     KGKVASIRENENGSGDLILRYEDVTRCIVKEAKHDLVVLSVGVIPNKDIQKIFTDQIL
                     QLDDFGFVKQVDELLNPASTNIEGVFVAGAASGPKDIPDSILSAGCAASEVAGYLATM
                     ESVNSVNQMKGNQLSISN"
     gene            19207..21414
                     /locus_tag="PLF20_01805"
     CDS             19207..21414
                     /locus_tag="PLF20_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit A family protein"
                     /protein_id="HOX79780.1"
                     /translation="MKERIGVYICHCGGNISDYVDVEELSKMFYKEDNIVVSKNVMFV
                     CADSSQKAMIADIQEYKLDAIVVCSCSPKLHLHTFRNVAACAGLNPFNYVQVNIREQC
                     SWPHSDHPHEATVKAAGLIRAGINRVSHSESLENIEIEVKKSALIIGAGVAGMKAAIE
                     LARSGNEVFLVEKDYFVGGHIAQKGQLFMSDQEGKDVITSLYHEIKKYPNITLFTGST
                     IEKVSGSLGSFHIELKVKPRYINPRVDKQTVKSVMDECPIEIDDPFNLGLVKRKVIYK
                     NYPEALPDLPVVDAEALKVFPDFVAKYKSVLNLTEEEQIISLMAGSVLVTTGYDDYLP
                     KEGEFGYKTLENVITLPELNRLMELNPNKLVYGGKEIKSIAFIYCVGSRQSKGENRFC
                     SRQCCTSAIYTSLQLKKKYKDIQAYHIYRDIRTYGKQEVLYEQSSKQGDLYFKYEEKE
                     MPVIEREGKSLIVKIKDYLTARKQLEIETDMVVLVTGMMPRKDALQISELFKIPVGSD
                     RFFNEIHPKLKPVETVIKGVYIAGACQGPKNITESVQSSLAATSKIIALLKKGSFSAD
                     PIIARIDMDACSWCDKCAEVCDYSALKMIEMNGKMVAGVNKAMCAGCGICAPVCPENA
                     IEIAQYTDKEIEAMIDGFLAKLEINEKEGGSDSTPKESAIRMEEYPQVWKEILAVMKD
                     GKYTIPQIAENSKLNSELVTYHLMTMNKYGIVVPDGMDDKEMYYYYKENEDSH"
     gene            21527..22588
                     /locus_tag="PLF20_01810"
     CDS             21527..22588
                     /locus_tag="PLF20_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOX79781.1"
                     /translation="MSKVNPKFAIELSKYGSNDFHACFNCGNCTATCSLSNAEDSFPR
                     ELIRYATLGLENELKASLKPWECYYCGDCSIQCPRQAEPGELMMSLRRWLTSKYDWTG
                     LSGVFYRSLSVTILAFVLIFAAVLILADSFAWNLQNMMHVGHLFEMTVIFMVLLVIFI
                     PNLLRMWYFTILKPNVKAHPKIYFIALSDLFVHMFTQKRMKDCETKDNFRWLEHFILV
                     IAYLSLLFTTVFLNWFNTESLFVVILGYVESFFIFIITIDFVSSRINKNKALNKFSQL
                     SDWLFVIWLMIMGLTAFFVRLFIDTGIIENNKWVYIIHFTILAQWALIIVPFGKWTHF
                     LYRSFAMYFSKIKSLAIAQ"
     gene            23077..24321
                     /locus_tag="PLF20_01815"
     CDS             23077..24321
                     /locus_tag="PLF20_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOX79782.1"
                     /translation="MDIESIKQRFGIIGNSPLLNRAIDIARQVAPTDLTVLVTGESGT
                     GKEFFPQIIHYLSPRKHGPYIAVNCGAIPEGTIDSELFGHEKGSFTGALDSRKGYFEV
                     VDGGTIFLDEVAELPLSTQVRLLRVLETGEFIRVGSSKVLKTDVRVVAATNIDVHAAI
                     EKGKFRQDLYYRLNSVPIIIPPLRERKEDIYLLFRKFALDFAEKYRMPPIQLDDEAQQ
                     MLINYRWPGNIRQLKNITEQISIIEQDRLVTAQILAKYLPDPHHHDLPVLYHKEFLSS
                     QEISERDLLYKVLFEMKKDINDLKKLVFEIINQNGITQEMKQNNAPIINKLVRDYKDD
                     PGFIAKSSPFEDVHYEVAGKIDEPIIEPEVIDDSLSLQKKEIELIKRALAKHNGKRKG
                     AAAELGISERTLYRKIKEFNLE"
     gene            24898..25467
                     /locus_tag="PLF20_01820"
     CDS             24898..25467
                     /locus_tag="PLF20_01820"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HOX79783.1"
                     /translation="MKNRSFTSSRSALIRGLIAILIGGLAVFIPGLTLEGIIFSAGLC
                     VLIGGLVSLIFALREKNLGAKNILLLQTVFNLIIGILFIAMPQNIVKFFVIILGIGLL
                     FLGAFQLMVGLSVRSRYGWSWFYFSISLIVIVAGIIMLYDPFKSAQALLIFLGVLLML
                     YGISELVMAWHLRKELKTLTEEGDIIEPE"
     gene            complement(25609..27183)
                     /locus_tag="PLF20_01825"
     CDS             complement(25609..27183)
                     /locus_tag="PLF20_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOX79784.1"
                     /translation="MKTNERNILLILLGSSFLIRFLLSFWLELGNDEVYYYTYALYPA
                     WSYFDHPPMVGWVIRIFSLNLLVQGEWAVRFASVALGTLNTYLIYVLGKFIKNREAGI
                     YAALLYTSSIYCFVIFGLFILPDTPQSTFWLLAIFAFLKSIISSELNKNSRSWLLAAG
                     VFTGLAMLSKYTSAFLWAGAGAYILFFNRKWLKSKELYISILLSALFFLPVILWNVQN
                     DWISFTYHGERVDFSKGRFRIDYLMSELGGQFLYNNPVAVVTVFIAAINFMKGKHYIS
                     KPQGNFLLLISFPIIFIFIIISTFRATLPHWSGPGYHALILMGGCWLAWKRETSRQKK
                     IPFHLRAALSFLILVLIAGIAQIQFSLLIKPDVQKGVFRGKKDFSLDMYGWRQMGKNF
                     SDIQQRDEKQGKIRPNAPIISFRWFPAANLDYYVAKPNQTYVLAMGNLERIHHYAWIN
                     QYRGGFHQGMDAWYIVSGRDYKDPSQLCEDCWATIVPTDTVPIIRNHDTVMYFLIYHL
                     KNLQTLPADPLTTIGN"
     gene            complement(27170..28369)
                     /locus_tag="PLF20_01830"
     CDS             complement(27170..28369)
                     /locus_tag="PLF20_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79785.1"
                     /translation="MIAYLVLFFIGLILLITLWYYTSCPVYTFSPPNPFKGDSLYNPY
                     MNISEKKWWKGNFQVQSRAWGGITSGRGNSNEKIEKIYSSLGYDIIATSDYQKINRFG
                     IRKPSYIPTYEHGYGIHKTHQICLGSQKVLWRDYPFYQGLNHKQHILNCLGIQNEIIA
                     IAHPDLRDGYLKEEMKYLSGYHLMEVFNRARTSLAHWDSALSSGYPAFLLANDDAHNV
                     TNPYEIGRYATFVQSDTLVARHILEALKKGRAYGVRIAYKEGETLEEKKLHALRLPSL
                     KEVSFSNDTLKIKVSPSASEFRFVGQNGVILKNVKETNFAYYVIKPSDTYIRTEISFP
                     SQDIIFLNPIFRYQGGKITWPIRYIINRQASFIHYILWGSGGLILLFMSGLLIKKHHF
                     RKAHENQ"
     gene            complement(28456..29691)
                     /locus_tag="PLF20_01835"
     CDS             complement(28456..29691)
                     /locus_tag="PLF20_01835"
                     /inference="COORDINATES: protein motif:HMM:NF014135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOX79786.1"
                     /translation="MGPFYIALIIFILTYTGIIFTRLPGVNIDRPSAAFFGAIAMVLF
                     GILSFDEAIAAIDFNTIGLLLGMMIIIATLQLDGFFAFVASQTIRIARTPFKLLIAIT
                     LFTGITSAFLVNDAVVLFITPLIINLCKNGQLNPIPFLIAEILAANTGSLMTMTGNPQ
                     NMLIGINSGIPYTEFLLRLLPITLSGLFIIILVIRLLYNKTFSQKHEFTFDKNVFQYN
                     FRSMQWSVIILAGVIVMFFFQHLLHLSIPLISLTGAALILIFGKVKPSIVIKQIDWVL
                     LLFFASLFIVVHGVEKVGLFDRFITGNLLVENSRSLVFIHIISLVFSQLISNVPLTVV
                     MLPMMKTAGSEILWLGLASASTLAGNATIIGAMANLIVIESAQNKGVKISFMDFFKPG
                     IIVSLSTLLISFLVLIAQL"
BASE COUNT         9066 a         5624 c         6182 g         8923 t
ORIGIN      
        1 actcgcttcc ccaaaggcgg tataacttgg gtaggattta taggagaaaa atagggattt
       61 atcatatttt acgtttgcag ctcagatttt ccgggaaatc agcattcgtg tgtaaggatt
      121 attaagaagt gaaaggcaag tgaggaggta ggtcattttt ttcttttaat tgcctgagct
      181 gttgaatgga taatcccgaa ggagaaacac tagcttgacg gcacatcagg taaattctgg
      241 ctgctttggc aattagttct aattggtcgc aggcatttgc cggactcgtt ccataagaaa
      301 gcacgccgtg tttttcccag attactacag aatgatgttt gatttcgtct aaagttttac
      361 gtgcaagttc ctggcttcca ggaggcaaaa aaggcacgaa tccggctcct tcgggtaaga
      421 aaagaatagt ttcgggatgc atggataaaa gaactttatt tagggcattt ttttcgttga
      481 gattgggaaa atgattcaaa gcaatgacct cggtgatatg agtatgtaat agtgtattat
      541 ttagtgaatc ttcttctatt aattgttgat gaatggccag atgggcaagt aactcggaag
      601 aaggttgcaa atgaaaggag tgatttgcca agggaatgat ggagtagctg tcgccatcgg
      661 acgaaatacg caaaagcatc atgtatttgt gcggaaactc agccagttct ctcatgcggc
      721 tgcctgtggc actcatcaat aatatctgtt ccgataaatt agggaacaag aaagggagtc
      781 gatgttgttc ttgtttttta tcgctgatat aatccatggt tcgaatatgt ggagtgacat
      841 tcatggaaat gttacccgca ttgcgttcgg cccaaccatg tgcccatacg ttatatgcta
      901 cctccgctat ttcctttaat aggggtttta attctggaat cgaaattttc atattgcttg
      961 agaggataag atgtgttgtg caaattcttg agaaatgtaa ttttcgtaat ggctacaagt
     1021 gttagcggca aaagatgaag caaccgacac gatctcggcc cattcacacg atgggatcat
     1081 gtttaaatta ttcggagata tttgacggcg atacaattca tacatcaagc ccgcattgaa
     1141 tgaatcgcca gcgccaatag tactcacagc ttcgacggca gcggctgcaa cacgagtttt
     1201 tgtgcattct ttgaataaga gaatctcatc actgccatgc gttataataa gaacttcaca
     1261 tcctagcgaa tggacttttt cgaaaagttt ctggcaagaa tccactcggt agatgtagtg
     1321 aaaatcttca tggctgcctc tgatgatatg gctgtatcgt atattttcct caataaaagg
     1381 taataattgt tcgagcgagg cgagatggtt cttacggaaa ttgggatcat aaatcaataa
     1441 agcaccactg tttctggctt gtttcagaat atttgtcatt tgagttctaa tccccggatc
     1501 gatcgagaag aatgaaccaa acagcacaaa atcgccgggt tcgaagtctg gagtatcaat
     1561 ctggaatcgt ttttcgggat aattcttgta aaaagtataa gaggcattgc ctttggtatc
     1621 tattaaagca accgaaagtg aagtatttcc atgataagat ttgcaaaacg atgtattgac
     1681 agcgttgttc gtcaagaaat tttgtgtaat ttctcccact ccatcgtttc cccattcact
     1741 gatgagaaat gctggcatgt gcaagcgacc gagacttact gcagtattca gcatggcacc
     1801 tcccaccatg gcggtttcca agtgttggtc ccgaaagatg atatccatta ctgtttcacc
     1861 tattgtaaaa attctgttca tttttataaa agtacgattc tatcgaaaag gtcaaaataa
     1921 gaagatggat cttggaggaa gcatataatt ttattgacaa acaatgatca ttgtaggatg
     1981 acttaatttt cataattcaa aaatgaaaca tgcagcgatt ttgaaaacat tctttgttca
     2041 gatcatgtgg atggcatact atatacacat gaacggagag tttcccctgt aacagttttt
     2101 cgaggaaatt ttgtgcattt ctcgatgaga taaacgtaac ccttttgcag cagcgaggcc
     2161 ttcgtacctt tgcaatgctt ttgagcgttc gggttatggc catgttcgaa agttgccatt
     2221 ccttttccga aaaccattgt gctcgtataa tcacgctaat aaacataccc acgattatgg
     2281 ctgccccaca ggtatcataa gtccagcaaa aacctccggg aatcctctct gagcgttctc
     2341 ttttttcctt tagatgctct tcttttttat agttatgatg gatagaatta aggtatgcaa
     2401 cagtgagagc catagaaatc aaaaaatgat gttgtggccc gtgaacggga attccaagat
     2461 gatcgaatgc ctgcaggtca atctcgtccg gatcgcttaa gcctgtatcc atgcagagag
     2521 tgaatatcat ttcatttatg agctgattgg tggagccaaa gcagatgtaa tgaaattgtt
     2581 tacaggtttt ttttagctgg ttatcctgct tacaataata gcctcttacc gaatggaatt
     2641 ctttagtttc taatatttga ttgccacaaa tccgacagct ttcttctggc atcgaatgga
     2701 gagatttatg gcaaacctat attttttctc tgaaatagcg aaatattatt ggtaggagaa
     2761 gaatgttgag gctgccctta tgtttattcg aatatgacgc gaatccttca tgccggttgc
     2821 tttcgacgat ctatgaatcg aagatcgaaa tggaacgact ggagaatagt ttataataat
     2881 ttgctcttcc tgatttcaga aggggaatga atcataacca atgcgggttg tttggtttcc
     2941 tcagatgata tcgtgtctat gagcatcgag cttgataaaa ataaacaata aaatggtaaa
     3001 cgccagtaaa gaagaacccc catagctaac gaagggcaag ggaatcccga ttactgggag
     3061 taggccaata gtcattccaa tgttaatagc gaaatgagcg aaaatgattg aagccacacc
     3121 atatccgtat attctcgaaa acgccgatcg ttgccgttcg gctaattgta atatcctgag
     3181 cattaacccc acataaagcc caatgagaat cagacttccc acaaagcccc attcttctcc
     3241 gatggtacaa aatataaagt cggtgctttg tttgggaacg taattgtatt tggtttgtgt
     3301 gccctttaaa tacccttttc cccagaaacc tccactacca atggcaatca tgctttgata
     3361 gacattatat ccagcgcctt ttatatcggt ttcctttcct aacaacacat ttatccggct
     3421 tcgctggtga ggctgtaaaa cattatggaa tacataatcc acgctaaagg ttatggaaga
     3481 agtagctata aatattccta tcaagatcca gtgaagcttt ttcctgtttc ttcgagtcag
     3541 ataataaacc agcagaatga gagcacatag gccaatgagg atgtatttat cgagaacgag
     3601 tgccagtacg aacagagcaa ttataatggc tcccgcaatg agaaccttcg ccgaaagtcc
     3661 ttcccggtat aatacgagaa tgaaactcaa aaacacgagt gccgatccgg tttcgttctg
     3721 tgctaatata atgagagccg ggatgagaat aatcgagaag gctgcaattt taacccctgc
     3781 cttgctgaaa ttccgtttta tgttcccaag ataggaggct aaggccagat tggtagcaaa
     3841 tttggcaaat tcacccggct gaatggaaat ggggcctatc tgcaaccacg acttcgatcc
     3901 tgaaatctct ttgccaacga ataagacgag aataagcgat acgattacca atccataaat
     3961 aaaaaacgcg aatgaattga ataactttac atcggttagc aaaatagcca tggcaataac
     4021 gaaagaagtg gctatccaaa tcatttgcct gccatagttg gtctgcaaat caaatatgtt
     4081 tttgtgaatc tcgtcgtaaa cagctgcata aatattcaac catccaaaga tgaccagggt
     4141 gaaatagagc cacaagagtg ttctgtcgat cccagcaaaa ggattatgat atttgtatcg
     4201 ggtagtgttc ataataaatt ggcttggctc attctttctt ctaattcggt acgactaatc
     4261 tttccggtga gatatttctc tatcatcaga gaggcaatag gtactgccca ggttgcacca
     4321 aaaccagcat tttcgatcac cacggaaatg gcaatcttag gattttcggc tggagcaaaa
     4381 gcaatacaaa ggctatgatc tttcccatgg ggattctgaa cagtacccgt tttaccacaa
     4441 atatcgatgt tctccagctt ataccagcgc cccgtgcccg atactccttg aaatactcta
     4501 tacattcctt tagtaatggt ctcgaaatgt tgtgaatcga tactgctttg tttcttattc
     4561 cagcgcgtgg gtttaccctg aacagatttt actacatgag gaggataata ataacctttg
     4621 ttggcgatta ccaccgcctg atttgccagt tgcagcggag ttataagaat ctctccctga
     4681 ccaatggcga gcgaacgtat agtcaaagct ttccagcctt tttctccaaa ataacgatca
     4741 taatactgag aacttggaat atttccctta acctgattgg gaatatcggt gtcgaaataa
     4801 ttaccaaacc caagactcat cacgttttgc cgccatatat tatacccttt atgaatatca
     4861 ccccctcccg ttttttctat ggtagttttg aaaacacccc aaaaataagg attacacgat
     4921 tgttctatag cccctatcaa ttcgagagga gagtaatgac tatgcgaaca ggcaatgggt
     4981 agactggctt tcccattgca atagaatcgg ctttctggag taatggcgcc agtttgaagt
     5041 cctaccaacg catttacaag tttaaaggtg ctaccgggtg aataagtcgc ttgaatagat
     5101 cggttgaata gcggattcaa aggatcctga ctcaatcgtt tgaagttttc tcctcttacc
     5161 cttcctacca agagattagg atcatatgaa ggtgaggaaa caatggccaa aatttcaccg
     5221 gtcgaaggtt ctatggcaac tatgcttcct ctcttattca tcatgagctt ttcccctaac
     5281 aactgcaaat cgaggtcgag gcttgtgata aggtcctgac ccgccatctg cggtttgtcg
     5341 tataatccat tctcgaatga acctttttca cgattgaata catccaccaa tacaatcttt
     5401 tcccccttca ctcccctgag ctctttttca taagatttct ctatcccact cttcccaata
     5461 tagtccccca tcctgtaata aggatcactc tctatttcct tttgatttac ctcaccaacg
     5521 tatccaaggg tgtgagcagc aataggataa gggtaagtac gaagcgtacg aggttgtacg
     5581 aaaaaccccg ggaaacggta gagcttttcc tgtaaatagc cataggtagt tttcgaaatc
     5641 tgttccacaa agggcgaagc tctatacggg ctatattgtc tggctttgtt taatcgattg
     5701 gtaaaacttt gcatatctat ctcgagtaat tggcatagct cggccgtgtc gatatttctg
     5761 acttgtcttg gaaccaccat cacatcataa gcctcttcat tcgaaacgat ctttctgcca
     5821 ttcctatcat aaatcaaacc ccttgccgga taaatggtta ctatgcggcg tacattgctc
     5881 tccgacagaa ttttgtactt gtcgctgatt acctgcatgt aaaataattt caaaataaaa
     5941 atagcggcca cggcgatgat gatccccgaa actacatacc ttttctgcga atgcgatgaa
     6001 agctgagaca tattgcaaat ttattgggta ttccctgaac ttttataatg gcgattcgtt
     6061 atgaactttt ttgagccgaa atcttaacta ctgaattttt tgcccatctt gttgcttttc
     6121 atcgtatcca acatttccca gaataaagcc cttctttaca agaagaatgt tacgggaaaa
     6181 tcaatgaatt atttgttgga tgattttatc tctttttacg gatgtatttt tatatatcaa
     6241 tgttaatcag caaagaagaa tgactttcct ttatcggtat ccttttgaaa aaacaataag
     6301 cgattttaaa taaacacctt atgtctacaa tcttttatta atcagcgata agatttaatt
     6361 ttgcttatta gcttatagcc caaaagatga aattgatcga aactcccata gaatatttga
     6421 aaggagtggg tccggttcgt ggagacctct tacgaaaaga acttcaaatt tttacctttg
     6481 aagatttgct ctattatttt ccttttcgtt atgaagatag gaccatcatt tacaggataa
     6541 aatccctgaa tccatcgatg aattacgtgc aggttatcgg taaggtagaa atgatggaaa
     6601 cgctgggatc gggtcgaaac ctacgcttgc atgtttacat caacgatgga acgggtagat
     6661 tggagctggt atggtttcag gggctcaaat gggcaaagca gctctttaaa tcagacctaa
     6721 cttatctcgt ctatggtaaa ttgaattatt ttaatggaac atacagcatc cctcatcccg
     6781 aagtggaaat ttatgaccct tcgaatgggg acgatataaa ggcactgtat ccggtttatt
     6841 cttctaccga aaagttaaaa agccgtggat tgggaagcaa aggaatctca aaactggtta
     6901 aaaccctttt gcttaccctc gaaggacaaa tacaggaaag tctggatgaa atggtaatcg
     6961 atgaaatgaa tcttatctct ttatcgaaag ctttgcagtt catccatttt ccacccgatc
     7021 agttttcatt ggaatctgct cggctacgct tgaaattcga ggagttgttc tatgcacagt
     7081 tatccgttct tcaattgaag catcaacgga agtcgtttag ccatggacat acttttcaaa
     7141 aagtaggcaa atttttcaat gaattctatg aacatcatct tccgtttgca ttgaccaatg
     7201 ctcaaaagcg tgtaattcgt gaaattaggg cagatttaag aggccctcgg caaatgaatc
     7261 gcttgctgca aggagatgtg ggcagtggta aaaccttagt tgccttaatg gtaatgctca
     7321 ttgcgctgga taataattat caaacctgct tgatggctcc caccgaaatt ctcgcacagc
     7381 aacattacaa tacctttctc gagatgcttt cgggaatgga ggtagaagtc cgtttgctca
     7441 cgggcagcac tcgtcaggcc gaacgtcagg aaattttgaa tggtctcttg gaaaacaggg
     7501 tgcagattct tttaggcacg catgccctca tcgaagatca ggtgcagttt gccaatctgg
     7561 gtctcgtagt tatcgacgaa cagcatcgtt ttggtgtaga gcaacgtgct aagctctgga
     7621 ttaaaaacaa tatcccacct cacgttctgg tgatgaccgc cacacctatt ccgcgtactc
     7681 ttgccatgac tgtctatggc gacctcgact attccgttat cgatgaaatg ccacccggcc
     7741 gtaaacccgt gaaaactatt ctctttaccg attccgatcg cctgatggtt ttttccttta
     7801 tgaaaagaca gattgccaaa ggaagtcagg tttacgtggt gtatcccatg atcaatgatt
     7861 ctgacaagct cgaattgaaa tttctggaag atggttatat cagcgttaca cgcgagttcc
     7921 ctttgccaga atatgccgtg agcaaggttc acggacaaat gaaacctcaa gacaaagaat
     7981 atgaaatgca aagatttcag aagggagaaa cccaaatcat ggtatcgact tccgttatcg
     8041 aggtaggtgt ggatgttcct aatgctaccg ttatggtcat cgaaaatgcg gaaagattcg
     8101 gattatcgca attacatcag ttgagaggta gagtagggcg aggagacaat gaatcctact
     8161 gtattttgat ggcaagcgat aaaattacgt ccgatgctcg tgcacgcctt gatacgatgg
     8221 taaacatcca cgatggattc aagattgccg atgttgattt gatattacga ggccctggtg
     8281 atttgaatgg gacacgtcag agcggaataa tgaaatttcg cctagccgat cttatagaag
     8341 ataaggattt actcgataaa gccagaaatt tagccgataa aattttaagc tatgatccct
     8401 tgctcactca accccaacat gccattgtgg ccgatcaact gaaaaacaaa cgtaaagact
     8461 tgctgcccct cagtatgatt agttgagctt ttttcctact tttgcgagct tttattacag
     8521 tcttatatct tgaagctatt ctgcttatct tatgaaaatc tcatataagt ggctacaacg
     8581 ttatattgat actccccttt cttatcagga gatttcggaa attcttaccg agagtggcct
     8641 cgaagtcgaa gccatcgaaa ccatagaatt tataaaagga gggctcaagg gcgttgtagt
     8701 aggtcaggta gtctcttgcc agaaacatcc cgatagcgac cacctccatc ttacaaaagt
     8761 ggatatagga aaccaagaac ctttgtcggt tgtttgtgga gctcctaata ttgcagaagg
     8821 acagaaggtg ttactcgcta aagttggaac tactctttat tttaaaaatg aaccaatggc
     8881 gattaaaaag gcaaagataa ggggagtggt ttctgaggga atgatctgtg ccgaagatga
     8941 attgggtctt ggtacttcac acgaaggcat tctggttttg cctcccgatg ctccgatcgg
     9001 tatgccagcc gcccaatatt ttcatctcaa agaagatgtg gttttcgaaa taggtttgac
     9061 tcctaaccgc accgatgcta cttcacatat aggtgttgca cgtgaccttc acgctagcat
     9121 aatcgcccgc aggcccgaaa ttccatctca ttttcaaaaa ccttcggtcg agaatttcaa
     9181 aatcgataat catgacttcg agatcgaagt ggaaattgca gatgctcaag cttgcattcg
     9241 ttattcggga ctcaccctga caaatgttcg agtaggagaa tcgcctggct ggctcaaaga
     9301 actgttgcag tcggtaggaa tcagacccat taataatgta gtcgacatta ccaacttcgt
     9361 tttgatggaa actggccaac ccctgcatgc ctttgatgcc aacaaaatca agggaagaag
     9421 agtgattgtg aaaaccttac ctgaaggaac tccttttaca actctcgatg gcatcgaacg
     9481 aagactcgat tctaccgacc tgatgatatg caatgccgaa gaaggaatgt gcattgctgg
     9541 tgtattcggg ggccttgaat ctggtatctc tgcttccaca caaagtgttt ttctcgaaag
     9601 tgcatgtttc aatccttcct atatcagaag aacttctaga cggcatcaat tatttacgga
     9661 tgcttcattc cgttacgaaa gaggaagcga ttttaacatt accatctatg cattgaaacg
     9721 cgcggcaata cttcttcagg aaattgcagg ggctaatgtt tcgtctgaaa tcaaggatgc
     9781 ctatccttct gaaattgaac ctgtcaaagt cgatttgcga tattctcaag tgaaacgtct
     9841 tgtgggtcaa ttcattccca aacgacggat actatccata ctccaaaatt tagaatatca
     9901 aattttaaga gaatcgccgc aatcattact gataagcgtg ccaagttgcc gtgtcgatgt
     9961 aacccgcgaa gccgacgtaa tcgaagatat tcttcgtatt aatggttata atcggattgc
    10021 ttttcgagaa gaattgcata cttcactctc ctatttctct catcctggtc ctgagcaggt
    10081 taaatgggaa attgccggcc ttttggccgg gaatggtttt tatgaagtaa taaataatag
    10141 cttgagcttt tcacagcatt atacactttg gcctgaagat caacggagaa ctctcataaa
    10201 attgttcaat cctcttagcc gcgaattgaa tgttatgcgg ggggatatgc tacctggact
    10261 tctcgaaaat gttgccttca atttgaacca tcaaatcgac gatttgaaaa tttttgaatt
    10321 tgggaagata tatcgtttcg atggaaacct tgaccctaat gctgatgtaa gcgttcgata
    10381 tactgaaccc cagcagcttg caatagcagt aactggtttg cgggaaaatg agagctggga
    10441 gaaagaccat cgtcgggccg atttttatta cctgcattct attcttagaa aggtattgca
    10501 acgtgtaggt gtcaatttgc gcttgctgaa aatcgttgaa tcccaaattc ctttttatga
    10561 tcaaggtata gaactgtttt tcggaaatga gcctttggca tgggtaggaa ttgttgccaa
    10621 ggcaatgctc caaaagttcg atatacgtca ggaagttgtt gcagccgttg taaactggga
    10681 taaagtttca tcattcccca gagaaatgaa tatcgaattc gaggaaatac ctcgttttcc
    10741 tgaggtacgt cgtgatctgg ctcttttact cgacaatcat ataaaatttg ctgaaataga
    10801 agctctggct tggaaaaccg aaaatcgcct ccttcgacaa atcaatcttt tcgatatcta
    10861 tgtaggtgaa aacatcggac caggtaaaaa atcctatgca ataagtttta tccttcaaga
    10921 taaggagaaa acgctaacgg atgaggatat cgataaaacc atggcaaaac ttatcacggc
    10981 attcgagcag aagttgggtg cacaactgcg ctaatacccc aaaactcgaa tcctcgattt
    11041 ggttttatac cctaagaccc aatgacgaac ttattcatta ttatccaatt aattggaagg
    11101 atagtatact gttcatctaa aagattttta acataaactt tgtccttttg cgatttttat
    11161 ctactttggt ggccttattc taaaatctat tcttatctaa atttcaataa aatatgaata
    11221 acaacaaaac cttggtttat tctgtcagcc cttcaggtga agagtttcca ctacctcaaa
    11281 atgatgatta tattgctgaa agaaagcgta ttgagtcatt agttggggaa gctcgtagtg
    11341 aaagaaagga aatcgttgtg gtaatgggat taggttttgt aggagccgtt atggcggcaa
    11401 tagttgccga tacccgcaat agcatgggag agtattcgaa atttgtaata ggatgccagc
    11461 gtccaagcaa aagaagctat tggaaaattc cgattttaaa cactggtgtt tcacctatta
    11521 aagccgatga tccggaggtg gatgaattga ttcatcggac cgttgttgaa aagcaaacat
    11581 tgacggctac tttcaatgat gattgtctcg aatttgccga tgttgtcgtc gttgatgtac
    11641 agtgtgattt tactaaattc gaactcggaa atatgcgttc aggcactgcc gatatggctg
    11701 ccctcgaagc caccatgaaa acgattggta aaaagatacc gcctcagtgc ctcgtactta
    11761 tcgaaactac cgtagcgcct gggactaccg aatttgttgc ctggcctata ctgaaaaaag
    11821 catttgccag acgtggaatc gaatcccaac cattgctatc tcatagtttt gaacgtgtca
    11881 tgcccgggaa agaatacgtc gccagtattc gggatttctg gagagtagcc tctggctgca
    11941 accttgaagc tcgccaaaga gtcgaaaaat ttctcagaga agtcgtcaat accgagaaat
    12001 ttcctttaac catcatggat agacctatcg aatcggaaac caccaaaatt gtcgaaaatt
    12061 cctatcgggc taccattctt gcctatctta atgaatggag tctgtttgct gagcgtaatg
    12121 gagtcgacct gatcaaagtg atcgatgcca ttaaaatgag gcctacacac agcaatatta
    12181 ttttccccgg ccctggcatt ggtggttact gtctgccaaa agacggagga ttaggctatt
    12241 gggcttacaa acatattttg ggattcgatg acgccgataa actttttcgc attacccctg
    12301 aagctatcga catcaatgat acaagaagtt tacatgtagc cgaactcacc cgcgacgcct
    12361 tgcgtaatat gggacgttat atagccggct ctgacgtgct tgtttgtggg gcctcctacc
    12421 ggcaggatgt gggcgatacc cgttacagcg gaagtgaaat tatcgttagg aaacttactg
    12481 aaatgggcgc cgatatacgc gtacacgacc ccttcgtgga tgtttggtat gaatttgtca
    12541 atcaggatga aaatgcacat tcatggaaac gtttcttcca ttatcaggat ggattgaaaa
    12601 atttgcagat tctcaaggat cttcctaaag ccatggaagg ggttgaagca gtcgtattgg
    12661 ctgtacctca cgcacctgac gatgcagtaa aatgggcggg aggtaaaatt gcggttatcg
    12721 attgttttgg tattcttcct gatgacaaga ttcgccgcta tttccagctc ggttgcgagg
    12781 taaaagcctt aggaagaggc catattcaga gaattaagcg tgaagtcagg ggactggaat
    12841 aattttgttt cgtacatccg aggaagtctc catttcatca gttagaattt ttatactgag
    12901 agagctttat tttgacaagg tttttaccga gtgtaattcc ttttcaagtc taagacagtg
    12961 gttcttaata tgaagaagat atttttcgta agaagttttt tgtggagatg cttatgatgc
    13021 ggccgaggcg aggcctcggc cgcatcataa aagcagtacc atcgcaacat catagtaaca
    13081 aaaaatctca tccacatctc gtaatttata ccaaatctat ttaaatttac ataaaaattt
    13141 taattatgca attcaaactg tttcttatga ttacaattgt tttagcttca ggcattgcaa
    13201 tagccggaca atatgaaacc gataccttcg agacgaaaaa tcaccacaaa ctgaccatta
    13261 actttatcaa acacggaagt ttgatgatca atttcgatga ttttatcata caagtcgatc
    13321 ctgtgagtat gtttgccaat tattcgactt ttccaaaagc tgaccttatc ctgattacac
    13381 acgaacatga cgatcacctc gacactgagg ctatcaaagc attaatgaaa cctaatacct
    13441 tgctcatcgc taacgaggca agtctaaaaa gactgggaaa attaaagact aaagtcatga
    13501 aaaatggcga cagactcgat gtttcgaatc aaatcagcat agaggctata cctgcttaca
    13561 acaccaccaa aggtcatgag cattttcatc cctctcatcg tgataatggt tatattttaa
    13621 ccatagatgg attgaaaatc tacattgctg gcgacaccga agatattcca gaaatgaaga
    13681 atttgaagaa tatcgatatt gcttttctcc ccgtaaatca gccctatacc atgacagtag
    13741 ctcaagctat caatgccgcc aaaataattt cacccaaagt gctttatccc tatcattttg
    13801 gtaatactaa tgtaaaacaa attcaggaag gcttgaagaa tagtggaatc gatgtgcgat
    13861 tgagaaaaat ggaataatct tctacaaaac tgaaatcttt gcgttgaaat aaatccctat
    13921 ccttttctgt cttttcgaag ctacaagcaa tgagccgctt ataaccaaga cgcaagcgac
    13981 tatccatttt ggcttatcat ctttagagat cgagtattta gaatttctat tttcttccct
    14041 tccattcgta taatcccatc cgacatgaat tctgaaagca cacggcatac gctttcacga
    14101 gtggtatcca ctaaattgcc tatctcggac tgagaaagag gaagcaagaa tttgttggat
    14161 tcaaaaatgt tttccgaaaa atcaatcaat acatcggcaa gattacctct gagttgtttt
    14221 tgtgtacgat tggcacaatg acgaaatgat tcgacctcac ttctgcataa ctctaataga
    14281 atataattgg aaaaatcttt gttttgttcg agcatgcgat tgaaaatatt caaatcgatg
    14341 aagcaaactt cgcaatcaag caatgctgta atcgaatact ggtttatttt atcgcctatg
    14401 gtagttggga ggccaagata ggaaggccgc ttgattaaac gaacaatttg ttcacgataa
    14461 ggcccagtaa tatgaatttt tacaagccct tttctgagga ataacacatt ggtagaaaaa
    14521 gttccctgtt ttattatggg atatcctttc ttgaatctta caacgacatg attgtttccc
    14581 aattcttctg cctggcattt gtttaaggtg cggaaatatt ttacgatgca tggacaatcg
    14641 atacattcat ttttcatgct gcaaatttaa agaaaaagtg atacaaatca caaaatgatg
    14701 ttatatcatt cacattttat ctgtagagac ggtaaagaaa tattaattta ttttgcaata
    14761 attaattcct taaattttct tgaaatggaa agcattgatc agacaaccat agaaaagctc
    14821 aaagctgaga tcgaagcttt gaaatcaacc tccaaacgtc tcgaatcgtc aagaaaagac
    14881 caattgtcga ttgcaattat ttctggcgat atggataaga ttatggcagc catgattatc
    14941 gcattggccg ctgcagcaat ggataccaag gtaaaattgt ttttttcttt ctgggcgatt
    15001 ggtgccctgc gtgatccagc aaaacatcct aaagggaaaa atttcatttc aaaaatgttt
    15061 ggatggatgc ttcctaaggg ggtcgatcat ttgaagctgt cgaaatttaa tatgtgtggc
    15121 atgggtccat ggataatgaa aaaactcatg aatcaacaga atgttttatc gctgagagaa
    15181 atgctgaaag aagctgacga attaggtgtc gaaattactg tttgcgaaat gaccatgaat
    15241 ctgatgggat tcaaaaaaga agaattcatt gattaccccc accttcgttt tgccggggca
    15301 gcaacctttg ttgccgaagc tggcgaaagc agtatgcaat ggatgatatg atcgaaagca
    15361 aatcttgaag tttatcctcg atcaaagtag aaatcaatga atatatcaac cttgataact
    15421 ctatactgta ttgagaaaat tttaaaactt aataaaatca taatcaaatg acaaaagaag
    15481 aattaaagaa cctgaaggtg gcaaaatctg ttgatgcccg tggaacggct tgcccaggcc
    15541 ccctcttggc tgcgaaaaaa gctataggtg atatcgaatc tggtgaagtt ttagaaattc
    15601 tttccgctga tgaaggaaca aaaaatgata tcccgcgttg gtgcgaaaaa gttggccatc
    15661 aattccttgg ctatttcgat gaagacagtt acagccgttt atttttgatt aaaggatgac
    15721 gtataatccc cttatttctg atttctaaca ctctttgctt cagaaataaa ggactatatt
    15781 tttcgctatg gtaatatccc caaaaatttt ggttttttcc actgataaga tttccgatcc
    15841 tggaatcgat caggcgggtt tgagaaaaat ccattattct ccttcggtat atgtaattag
    15901 cttgccttgt tcatcaggtg tgaagcctaa gtggattatg catgcattgg aaaatggctt
    15961 cgatggagtt tttattgctg ccgatggcca cgaatgctcc tttagttccc gctgtgccga
    16021 atatacgaac aaaatcgtgg aagtttctca gcagctcatg aaagccaaaa atatcaatcc
    16081 aaaacgtatc agaatggctg ctctttgttc ggtgtgtgcc gaaccatttg caaaccatat
    16141 ggaaaatttt ggaaaaattc tcgcaaactt ggaagactaa tctccagtta aaactttctg
    16201 aacaaagcaa tgaatccttt ttattgatat ataataataa atatttacat atttaattga
    16261 gattctttta ttgaagatat tctaaacctt aaccattatt tcattattct aaatttgatg
    16321 gataaacaag aaagtaagta tgatgcgttg gtagtggggg gaggaatagc aggccaggaa
    16381 atggccttga gccttgccga tatgggctat caggtattgc tggtcgaaaa ggggctgtct
    16441 ataggaggga aaatgattca attgagcaaa gtatttccaa ctttagattg tgctgcgtgc
    16501 attacaaccc caaaaatgtc agagacgtcc catcatccta atattagact attgctcaat
    16561 agcgaagtgg aggagatcaa gaaaaatgga actgaatttt ctattcgaat gatccaaaag
    16621 ccacgattcg ttattcagga aaactgtacg ggatgtggag aatgtgaggc aatttgtcca
    16681 gagatacgca atgacgatta taacgctaat ttagccggca ggaaagttgc ctatatccct
    16741 ttcagtttgg ccaatccacg tattgcagct atcgatcgac aggatcattc ggctccttgt
    16801 atcaacgaat gtccgggcgg agttaaggct tatggttata ttaccctagc tcgtaatggt
    16861 caatacgaag aagcaatgaa acttcatctg gaagatatac cgtttccggg gagtcttggc
    16921 cgtgcctgtt atgctccttg ccaaaatact tgcacgagag gtaagctcga aggccctgtc
    16981 gatattagaa aaatcaaacg ttttttttct gactggtatt atgaaaaata tcccgaaccc
    17041 cttgctgttg aaattaggga aaaatcgggg aaaagaattg ctgtaatcgg ttctggacca
    17101 gccggaatga ctgctgccta tcatcttgca ttaaaaggac atacagtaaa aatattcgaa
    17161 gccgctccac aacctggagg tatgttgcgt cttggtttgc ccgaataccg tgctcctaag
    17221 catatagttg atcgagacct taaaaacatt actgttcttg gcgttgaaat agaagtaaac
    17281 aaagaggtcg ccgatatcaa ggcacttaaa gaacaaggct ttgatgctgt ttttatcgca
    17341 gtaggaacac acgaagcatt tacttcgaaa atagaaggat ctgaattgaa gggggttttc
    17401 gattgtctta aattcctccg cgaagcaaat attggtaaaa aggatgacct gaccggtaag
    17461 aaagtcatgg taattggagg tggaaataca gctatagatg cagcacgaac ctcgttacga
    17521 ctgggtgctg aaaaagttac catagtttat cgccgtagcc gcgaggaaat gccatgtttt
    17581 gctccagaaa ttcaagaggc tgaagaagaa ggagtggaaa tactgatttt gagtaacccg
    17641 gtgaaatata taggtgaaaa tgggtggctt aaacaagtgc agttgataaa aatgcgtctt
    17701 ggggagaagg attcttctgg ccgtcgaagc ccagatccca tccctggctc cgaatatatt
    17761 cgggatgtcg attatgttat tgaagcaatt ggcatgaggc ctcataccaa gccttttgcc
    17821 gggcaaattg aactaaataa gaacggcacc attaaagttg atgaaaagac cttacaaact
    17881 tcagttgaat atgtttttgc cggcggagat acggtgatgg gtgctactac tttaatcgaa
    17941 gcggctggtc agggcaaaaa agcggccttt tatatggaca aatatcttcg aggcttaaat
    18001 atgtgtgatt ttgaatatgg agataaattg cctgcggttg acaaatcatc catattaagg
    18061 agatacaagg gctctgttaa gcctccaatt ctgggcgaaa tgttgtcttc gaatcaccgt
    18121 ataaaaaatt gggatgaaat cgaaaaagga tacacagaga ctgaactgaa agccagtacg
    18181 gatcgctgcc tcgattgtgg caattgtcgc gaatgtcatc agtgtatttc ggcttgtccg
    18241 gccaatgcca ttgattttag ccagaaaact caaatcgtag atattacggc aaaatctgtt
    18301 attttaacaa ctggctataa attgtttcca ccaaatggca agccaaatta tggttacaca
    18361 aaatatgcta atgtgattga ttccttgcaa atggatcgtc ttattgcccc cacacgtccc
    18421 tttaataatg tacttcgacc gggcgacgga aaaattcccg ataacattgc ctatatactt
    18481 tgtacgggtt cgcgcgattc tgccatagaa aattcagttt gtggggatga gtgttccaac
    18541 aatcctattt gttcacagat ttgctgcatg tattctatca aacaggcaca gcttttaatg
    18601 ggtgccttgc ctatggccga tgttacagtt tattacatgg atatacgtgc ctttggaaag
    18661 ggttatgaag aatttttcca acagaccaag tcaatgggag taaactttat aaaagggaaa
    18721 gtggccagta ttcgtgaaaa tgaaaacggt tccggcgact tgattttgcg atatgaggac
    18781 gtaactcgtt gtatagtaaa agaagccaaa catgacttgg ttgtgctttc agtaggtgta
    18841 attcccaata aagatataca aaaaatattt acagatcaaa tccttcaatt ggatgatttt
    18901 ggttttgtaa aacaggtaga tgaactttta aatccggcat caaccaacat tgaaggagta
    18961 tttgtggcag gagctgcttc tggcccgaaa gatattcctg attctatctt atccgcagga
    19021 tgtgctgctt ctgaagttgc gggatatctc gctactatgg aaagcgtaaa ctctgtcaat
    19081 caaatgaaag gaaatcagct atcgatttcg aattaggaga ttggctacaa caataaaaag
    19141 aaaaaagaaa agatgtcttt caatgttttt aaataattgg ttcaaatgtt taacttttag
    19201 agaacaatga aagaaagaat aggtgtatat atttgtcatt gtggaggtaa tatttctgat
    19261 tatgttgacg tggaagaact cagtaagatg ttctataaag aagacaacat agtcgtttcg
    19321 aagaatgtta tgtttgtttg tgctgattcg agccaaaaag ccatgattgc agatattcaa
    19381 gaatataaac tggatgccat agtagtttgc tcctgctctc ccaaacttca tctgcatacg
    19441 tttagaaatg ttgctgcatg tgctggatta aatcctttca attatgttca agtgaatatt
    19501 cgtgaacagt gttcttggcc acacagtgat catccgcatg aagctacggt gaaagctgca
    19561 gggcttataa gggcaggaat caaccgagtt tcgcattctg aatctttgga aaatattgag
    19621 attgaggtta agaaatcagc tttaattatt ggcgctggag tagctggtat gaaggctgcc
    19681 atcgaattgg caagatcggg aaacgaagtt tttctcgtcg agaaggatta tttcgtggga
    19741 gggcacattg ctcagaaagg tcaacttttt atgtcggatc aggaaggaaa ggatgttatc
    19801 acttcgctct atcatgaaat aaaaaaatat ccgaatatta ctttatttac gggttctact
    19861 atcgaaaaag tatccggcag cctcggtagt tttcatattg agttgaaggt aaaaccacgt
    19921 tatatcaatc caagagttga taaacaaacc gttaaatctg taatggatga atgtcccatt
    19981 gaaattgatg atcctttcaa tttgggtctg gtgaaacgaa aagttatcta taaaaattat
    20041 cctgaagccc tgccggatct acctgtcgtc gatgcagaag ccttaaaggt tttccccgat
    20101 tttgttgcaa aatacaagtc ggtacttaat ttaacggaag aagaacaaat tatatccttg
    20161 atggcagggt ctgttctcgt gacaaccgga tacgacgatt atcttcctaa agaaggagaa
    20221 tttggctaca aaacactcga gaatgtaatt acacttcccg aacttaaccg acttatggag
    20281 ctgaacccaa ataaattagt ttatggggga aaagaaatca aaagcattgc ttttatttat
    20341 tgtgtaggaa gccggcaatc aaaaggcgaa aacaggtttt gttcgcgcca atgctgtact
    20401 tccgcaatat atacctctct tcaattgaag aaaaaatata aggatattca agcatatcat
    20461 atttatcggg atatcagaac atacggtaag caggaggtat tatacgaaca gtcgtccaaa
    20521 caaggcgatt tatattttaa atatgaagaa aaggaaatgc ctgtaattga acgagaaggc
    20581 aaatcactca ttgtgaaaat aaaggattat cttacagcaa ggaagcaatt ggaaattgag
    20641 accgatatgg tagttttggt taccggaatg atgccccgca aagatgcatt gcaaatttcc
    20701 gaattattta aaattccggt aggtagcgac aggtttttca acgaaattca tccaaaacta
    20761 aaacccgtgg aaactgtaat aaagggagtt tatattgcag gagcctgtca aggaccgaaa
    20821 aatatcaccg aatcagttca atcatcgctt gcggctactt caaaaataat cgctctttta
    20881 aaaaaaggaa gtttttcggc cgatcccatt atcgcccgaa ttgatatgga tgcttgttca
    20941 tggtgcgata aatgtgctga ggtatgcgat tattctgcat tgaaaatgat agaaatgaat
    21001 gggaaaatgg ttgctggcgt caataaggct atgtgtgcag gctgcggaat ctgtgcgcct
    21061 gtttgccctg aaaatgctat cgaaattgct caatataccg ataaagaaat tgaggcaatg
    21121 attgatggat tccttgcaaa attggaaata aatgaaaagg aaggtggctc tgattctact
    21181 cccaaagaat cggccatcag aatggaggaa tatcctcaag tttggaaaga aattcttgct
    21241 gtgatgaagg acgggaaata cactattcct cagattgccg aaaattcaaa attgaatagc
    21301 gaattggtta cctatcatct gatgacaatg aataaatatg gcatcgttgt tcctgatggt
    21361 atggatgata aagaaatgta ttattattat aaggaaaatg aggatagcca ttaaaaagga
    21421 ttttctgctc tttgagggat ggattaaaat tcgacaggct tgatttatag gtatgttttt
    21481 taaactgaat atgaattttc attgataaat tgaaagataa attgttatgt caaaggtaaa
    21541 tccaaaattt gctattgagt tatcgaaata tggttcgaac gattttcatg cttgttttaa
    21601 ctgcgggaat tgtacggcca cttgttcttt atctaatgcc gaggattctt tcccacgtga
    21661 attgatacgt tatgcgactt tagggcttga aaatgaacta aaggcttcgc ttaaaccatg
    21721 ggaatgttac tattgtggtg attgttccat acaatgcccg cgtcaggctg aaccaggtga
    21781 actgatgatg tcgcttcgtc gatggctaac ttcgaaatat gattggacgg gactttccgg
    21841 agtattttac cgttcgctct ctgttacaat cctcgcattc gttttaattt ttgccgctgt
    21901 tttaatttta gccgattctt tcgcatggaa tctgcaaaat atgatgcatg ttggccatct
    21961 tttcgaaatg acagttattt tcatggtatt attggtaatc tttataccta atcttctgcg
    22021 catgtggtat tttaccattc tcaaacctaa cgtcaaagct catcctaaaa tatatttcat
    22081 tgcactgagt gacttgtttg ttcacatgtt tacgcagaaa cgcatgaaag attgtgaaac
    22141 aaaagacaat ttccgttggt tggaacattt tattttggta atcgcctacc tttcgctact
    22201 ctttacgaca gtattcctta actggttcaa caccgaaagc ctttttgtcg ttattttggg
    22261 ttatgtagag agctttttta tttttataat tactattgat tttgtttcga gtcgtattaa
    22321 taagaacaaa gctttgaaca aattttctca gctctccgat tggttattcg ttatctggct
    22381 tatgattatg ggattaactg cctttttcgt acgtctgttt atcgatactg gtataataga
    22441 aaacaataag tgggtgtata ttatccattt taccattctt gctcaatggg ctcttattat
    22501 cgtgcctttc gggaaatgga cacattttct ctaccggtct tttgccatgt atttttcaaa
    22561 gataaaatct cttgccatag ctcaatagct aattgccgtc gattctattg ccgaaaaatc
    22621 tcaagagcgg aggtatttgg ttgctttctt actatactgt gggttagact tacttttatt
    22681 ctttagggta ttaatgattt cataaatcac ctcatacaaa gagtagctca aagaagttat
    22741 gtgcttgtgt aaggtgttta taattcagaa ttcatttatt ataatatatg aagagtttat
    22801 gcatttgtaa ctttgacata aaccataaag aatggagatg gattttttaa cctcttggta
    22861 aaatttcgtt caaatttcct caaaatgttt tatctttgtg gaatttcatt cttatggtgt
    22921 tttttcgaat gatgaatatt tcagagcctt tatatttaat ctttacaagg gttttcgaga
    22981 aagaaaatgc agaattggac ttgaggtgtt atattttatt tttctgtttt agtttattat
    23041 atttatttga acaaattaaa tcattctaac tctgctatgg atattgaatc cattaaacaa
    23101 aggtttggca taattgggaa ttctccctta ctaaacagag ccatcgatat tgcgcgtcag
    23161 gtagctccaa ccgatcttac tgtattggta actggcgaaa gtggaaccgg gaaagagttt
    23221 tttcctcaaa ttatacatta cttaagtcca cggaaacacg ggccttatat tgcagtgaac
    23281 tgcggagcta ttcctgaagg cacaatagat tccgaactgt ttgggcacga gaaaggttca
    23341 ttcactggag cactcgattc ccgcaaaggc tattttgagg tggtggatgg cgggactata
    23401 tttttggatg aagttgctga acttccactc tccacacagg taagattgct gagggtgctt
    23461 gaaacaggcg aattcatcag ggttggctca tcgaaagtat tgaaaacaga tgtgagggta
    23521 gtagcagcta ccaatataga tgtacacgct gccatcgaaa aaggtaaatt ccggcaagat
    23581 ttgtattatc gtttgaattc tgtccctatt attattccgc ctttacgcga gcggaaagaa
    23641 gatatttacc tgttgtttag aaaatttgcc ctcgattttg ccgaaaagta tcgtatgcct
    23701 ccaattcaac tcgatgatga agcccagcaa atgcttatca attatcgctg gcctggcaac
    23761 attcgtcaat tgaaaaatat taccgaacaa atcagtatca tcgaacaaga tcgcttagtg
    23821 actgcccaaa tattagctaa atatcttccc gacccccacc atcacgattt gccagtcctc
    23881 tatcacaagg aatttctttc gagtcaggaa atttcagaac gcgacttgct ttataaggtc
    23941 cttttcgaaa tgaaaaaaga tatcaatgat ttgaagaaac tcgtatttga aattattaac
    24001 cagaatggga ttactcagga gatgaaacag aataatgctc ctataattaa taaattggta
    24061 agagattata aggatgatcc gggtttcatc gccaaatctt cgccttttga agatgtgcat
    24121 tacgaagtag ccggcaaaat tgatgaacct attatagaac ccgaagtaat tgatgatagc
    24181 ctttcacttc aaaagaagga aatcgaactc atcaagcggg cattggccaa acacaatggt
    24241 aaacgtaaag gagccgcagc cgaactcggc atctctgaac gcaccctcta tcgtaaaatc
    24301 aaggaattta atcttgaatg attgatgaga taaaaactta ttgatttttc attctctcga
    24361 tttttatcaa tagaccaggt catgctgagg tgtttatttc aactcttcga agtttgactt
    24421 gaacgaacca catacagggc ttttgcgctt attggccaaa tcctgaaatc gaatatttta
    24481 ttaggtttcg tcatagatat gattgcagtt cgttcagata tatttttccc caattatcca
    24541 aaccaaactc aaatggcttt tcaatcgatt cacaagtgca ggcaagagta cccattggtg
    24601 caaccaccag tttgctttat ggtaatcatg aatcagcaaa aggattctta ttttcattcc
    24661 aacatttgca tgaatatcgt cggaaatatt gaggtcgatt actatgatag agagctgaaa
    24721 tttgtgatta attcaaaata aatcaattca ttgaagtctg gttattgaat aaatggatac
    24781 tcatcgaagt cgctaatttt atagctcata tatactgata aatcattatt tggtttatcg
    24841 atcttgttta ttataggttt ttgtataatt tcgctttaaa cttaaaaaac atattctatg
    24901 aaaaacagaa gttttacgag ttcgcgcagt gctcttataa gaggcctaat tgccatattg
    24961 ataggaggat tggctgtgtt tattccagga ctgactctcg aaggcattat cttttctgct
    25021 ggtttatgtg ttttaatagg aggtcttgtc tctttaattt ttgctcttag agaaaaaaat
    25081 ctgggagcta aaaatattct gcttcttcaa acggtgttca atttgattat agggatatta
    25141 tttattgcta tgcctcaaaa tatagtgaag ttctttgtca tcatattggg cattggtctt
    25201 ttgtttttgg gtgcatttca gctaatggtt ggattgagcg ttaggagccg atatgggtgg
    25261 tcgtggtttt atttttccat ttctctcata gttattgttg cgggcatcat catgctatat
    25321 gatccgttta agagtgctca ggccttgctc atttttctgg gtgtattgct tatgctctat
    25381 ggaatttctg agttggttat ggcgtggcac ttgcgtaaag aattgaaaac cctcactgaa
    25441 gaaggcgata ttatcgaacc tgaataagaa aatcttgtcg acagtataga cttttccctt
    25501 tacgtctgct ttatgcatgg cttacctgtt tttcatgtca gaagcatttg gccatacatt
    25561 ttacggttga taacggggga gagaggatct cccttcatag gatgtgtact aatttcctat
    25621 ggttgtaagg ggatcagcag gcagagtttg taaatttttc aggtgatata taagaaaata
    25681 catcacagtg tcatggttac ggataatggg tacggtgtct gtcgggacta tggttgccca
    25741 gcaatcttcg caaagttgtg aagggtcttt ataatctctt cctgaaacta tataccatgc
    25801 atccattcct tggtgaaaac cgccgcgata ttggtttatc catgcgtaat gatgaattcg
    25861 ttccagattg cccatagcca atacataagt ctgattgggt ttggctacat aatagtcgag
    25921 gttggcggct gggaaccatc gaaagctaat aatgggtgca ttgggtctga tttttccttg
    25981 tttttcatct ctttgttgaa tatcggaaaa atttttcccc atctgtctcc aaccatacat
    26041 atcgagtgaa aaatcttttt ttcctctgaa aacccctttt tgtacatcgg gttttatcaa
    26101 caagctaaat tgaatctgag caattcccgc aatcaagact aatatcagaa aggatagtgc
    26161 tgctcgaaga tggaatggaa ttttcttctg tctactcgtt tcgcgcttcc acgccagcca
    26221 gcatcctccc atcaagatga gagcatgata gccaggcccg ctccaatggg gcaatgtcgc
    26281 cctgaaggtt gaaatgatga taaatataaa aatgatagga aatgaaatga gaagtaaaaa
    26341 attgccttgg ggcttgctga tataatgttt acctttcata aaattgatgg cggctataaa
    26401 gacagttaca actgccacag gattgttata gaggaattgc cctcccagtt cgctcatcaa
    26461 ataatctata cgaaatcttc ctttcgagaa atcgactctt tctccatggt aagtaaagct
    26521 aatccagtcg ttttgaacgt tccagagtat tacgggaaga aaaaataagg ctgagagaag
    26581 gatggagata tataattctt ttgatttcag ccatttacgg ttgaagaaaa ggatataagc
    26641 tcctgctccg gcccataaga aggccgaggt atatttggaa agcatggcca aacccgtgaa
    26701 gacgcctgct gcaagtagcc atgaacggga gtttttgttc agttcggatg agataatact
    26761 cttcagaaat gcaaatattg ccagcaacca gaaagtgctt tgaggagtgt cgggcagaat
    26821 aaataaaccg aaaataacaa aacaataaat agaagatgta taaagaaggg cagcataaat
    26881 gcctgcctct ctgtttttta taaatttacc gagaacgtaa attagataag tattgagtgt
    26941 tccaagagct accgaggcaa atcgtactgc ccattcgcct tgaactaaaa gattgagcga
    27001 aaaaattcgg ataacccatc caaccattgg cggatggtcg aaatatgacc aggcaggata
    27061 aagtgcatag gtgtaataat aaacttcgtc attgccaagc tctaaccaaa acgacagtaa
    27121 aaatcttatc agaaatgagc tccctaacaa aatgagaagt atatttcgtt cattggtttt
    27181 catgcgcttt cctgaaatgg tgttttttga ttaataaacc tgacataaaa agaagaatta
    27241 accctccgct tccccagagt atataatgta taaaactggc ttgccgatta ataatatatc
    27301 gaatgggcca tgtgatcttg cctccctgat aacgaaaaat gggattcaaa aagattatat
    27361 cctgagaagg gaagcttatt tcggttcgaa tataggtatc ggagggtttg attacataat
    27421 aggcgaaatt ggtttctttt acatttttga gaattactcc attttgaccg acaaacctga
    27481 actccgaagc tgatggtgat acttttattt ttagggtatc attcgaaaag cttacttctt
    27541 ttagtgaagg caatcgaaga gcatggagtt ttttttcttc taaagtctcc ccttctttat
    27601 aggcaattct tacgccataa gctctgcctt ttttcaaggc ttccaatata tgtcgagcaa
    27661 cgagtgtatc tgattgtaca aaggtggcat atcttccaat ttcgtagggg ttggttacat
    27721 tgtgagcatc gtcgttggca agaaggaaag caggataacc cgaactaagg gcactatccc
    27781 aatgagcaag actggttcgc gctctgttga atacctccat caggtgatac cctgataaat
    27841 atttcatttc ttcttttagg tacccatcac gtaaatcagg atgggcaatg gcgatgattt
    27901 cattttggat gccgaggcag tttaggatat gttgtttatg gttcaaacct tgataaaacg
    27961 ggtaatcacg ccaaagaacc ttctggctac ctaaacagat ttgatgtgtt ttatgaattc
    28021 cataaccatg ttcataggtt ggaatatagg atggttttcg aattccaaaa cgattaattt
    28081 tttgataatc ggaggtagct ataatgtcat aacctaaact ggaataaatt ttttcaatct
    28141 tctcattgga attgcctctc cctgaagtaa ttcctcccca agctctggac tgaacctgaa
    28201 aattgccttt ccaccatttt ttctctgaaa tattcatgta gggattatac aaactgtccc
    28261 ctttaaatgg gtttggagga gaaaaagtgt aaaccgggca acttgtataa taccataacg
    28321 taatcaacaa aatcaggcca atgaaaaata ataccagata tgcaatcatt ctacccgctt
    28381 tttttatata tctaaaaaac ctttgaggtc ttctttttac cataatctac aaaattaagg
    28441 aaaaagtgaa tcctgttaaa gttgagctat caaaaccaga aatgatatca ataaggtgga
    28501 aaggctaact ataatgccgg gtttgaagaa atccatgaat gatattttta cgcctttgtt
    28561 ttgggctgat tctattacga taaggttagc cattgcacca atgatagtag catttccagc
    28621 caatgtagaa gctgaagcca gcccaagcca taaaatttcg ctaccggcag ttttcatcat
    28681 gggcagcatg actaccgtca aaggcacatt gctaattagc tgtgaaaaaa ctaaactgat
    28741 aatatggata aagacgaggc ttctactgtt ttctacaagt aaattccccg taataaaacg
    28801 atcaaaaagg cctacctttt ctactccatg aaccacaatg aacaaggaag caaaaaaaag
    28861 cagaagaacc cagtcgattt gcttgatcac tatggatggc ttgacttttc caaaaatcaa
    28921 tataagtgca gccccagtca atgatatcaa aggaatactg agatgaagca gatgttgaaa
    28981 gaaaaacatc acgataactc cagccagaat aataactgac cattgcatcg agcgaaaatt
    29041 atattgaaag acatttttat cgaaagtgaa ctcatgtttc tgtgaaaagg ttttattgta
    29101 tagtaacctg ataaccaata taatgataaa aaggcctgaa agtgtaatag gcagaaggcg
    29161 aagcaggaat tcggtataag gaatcccaga gtttatgccg atgagcatat tttgcgggtt
    29221 gcctgtcata gtcatcaaac ttcctgtatt agccgccaaa atttcggcaa tcagaaaggg
    29281 aatagggttg agctggccat tcttgcacaa attgataatt aatggagtga taaaaagaac
    29341 tacggcgtcg tttactaaaa aagcacttgt tatacccgta aacagcgtga tggcgatgag
    29401 taatttaaag ggagttcggg cgattcggat tgtctgggag gcgacgaaag caaaaaaacc
    29461 atctaattgc agagtagcaa tgatgatcat catacccagc agtaatccga tggtattgaa
    29521 atcgatggct gctatcgctt catcaaagct cagaatacca aagagcacca tggctattgc
    29581 gccaaagaag gctgctgagg gtctatcgat atttactcct ggcaaacggg taaagatgat
    29641 gcccgtatat gtaagaataa aaataatgag tgcgatatag aaaggtccca tccgtcagat
    29701 ttccatagtt ccgtaaattt gcacatcctt gtttaatcca aaaccctcca aaattaggta
    29761 atataatttc aaaaaagata aaataatcgg gtaaa
//
LOCUS       DAOORU010000012        25939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_2264_length_25939_cov_6.335729, whole genome shotgun sequence.
ACCESSION   DAOORU010000012 DAOORU010000000
VERSION     DAOORU010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25939
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2264_length_25939_cov_6.335729"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..2077)
                     /locus_tag="PLF20_01840"
     CDS             complement(314..2077)
                     /locus_tag="PLF20_01840"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HOX79787.1"
                     /translation="MKLFYHFLSTVICLIFLVLFPFVLLAQDTEFRSIHVIEQQYYKV
                     HPIPSDTSHGKPGRNKTNIPLKKGKMIFGYHPYWMNNSWGDYRWDLITDLCYFSYEVD
                     ASSGMPVTTNDFGNATVIDTALTKGIHVHLCVTLFSGHASFFEDALARKTLNEQIVHL
                     VKSRGIHGVNIDFEAVPSSQASSLTNYIHELRQYLDSTAPDCLLSMATPAVNWNNTFE
                     LSNLAQDVDFFMVMAYDYYWNLSTTAGPVSPLYPMETTYPYNVSQTIEYYFSQGIGKE
                     KILLGVPYYGRIWPVTQLEAPASTRGSGSAVTYRNVRNNPVFFSWSNRKMNFPSRSVY
                     YAYQANGWNHCFIDEAPSLLYKYQRVIASDLKGIGIWALGYDMGYDELWDAIQQAFSE
                     NGGMLCQDTIFDSGGFLMNPSIFITQNMSILPNYPGPYKLKFFDLNLQNSKITIFDRQ
                     QPLGTPLETIWPGNDSTEIISDYGFYITFEATGKHPQANYAMSWSCPSAAVPSENNMS
                     IIEMSYIPFLNQIQIKNSSIPLKLKSIHIYSLTGHEMLSNTFNSPPYIFQLPVKLKSG
                     IYIIQLFSENIILTKKLLITQ"
     gene            complement(2111..2677)
                     /locus_tag="PLF20_01845"
     CDS             complement(2111..2677)
                     /locus_tag="PLF20_01845"
                     /inference="COORDINATES: protein motif:HMM:NF022751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HOX79788.1"
                     /translation="MIIIDGIMVQDEIIRCRFTCDLTRCHGACCIEGDAGAPLFADEI
                     NLIENCLEQVIPYMTEKGVATIKKSGIFEFDQSGQLVTPLIDDGDCAFINFENGTAVC
                     AFELAFQAGKTNFQKPQSCFLYPIRVGRSVTGDFLIYHKWNICKPALKKGKSLGIPLY
                     VFLKKPLIQRYGEEWYNKLIAYAKENPE"
     gene            complement(2849..5932)
                     /locus_tag="PLF20_01850"
     CDS             complement(2849..5932)
                     /locus_tag="PLF20_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HOX79789.1"
                     /translation="MIPVKLTLEGLFSYQQRQEINFRPLVEARLFGIFGPVGSGKSSI
                     TEAILYALFGDIERLNSREARNYNMMNLRSNRLWIDFIFNAGNPSQQYRITVEGRRKP
                     KNYHETESLDRKAYQKSNNTWLPIALEKIDEVIGLSYDNFRRTIIIPQGKFQEFLQLG
                     GADRIRMLKEIFQLEQFDLYNKAKNLFEKNHEILIQVNTRLESLSATPEAITTLQDEL
                     NTITAALLQERQKYSELQKRKDQQALLYEKNQQWQGLKLQFEQLNKEAEVMERNRNFI
                     EKYQMAESSFRSPLDLLDEISNRVNSLEKRCKELSHQIEILQPQRNNIQNQLALWKQK
                     SEELPFRKVFVEKIEELLHYRKIQQQIIEINPKIQALIDQIKSHDETLKSKKEEKSSI
                     LKEYEELETQLPNPNEIKQINQWFTEYDRLLEAKQQTEVRKQSALNDMEIIKQDALQN
                     SHLILCHELLAQKSIPEVLEEIQRNYNLSKNQLDEIRNQIQSLKVTTKLADFAKELRE
                     GMPCPLCGSTSHPHLFSAPEVEAKLKKLESTEIQLKAQQQAIIDAMSELKQYLGKLKA
                     KSDEIEKANRGLEKLTQQIDLHFNTYHFDGLEVGNKKAYQEFSEKIEFLQNRLRKLNL
                     NRNTLEQEIHNLEEKHRKQSTEMNQLQQQINFLSGQAEMLKNKYGGREWEENFIHSDD
                     ELQNIIKTTLEEIKSVKKELQQIEEQAEKIQHHWDQVNAELKSKHIDLEEAKEKLNVQ
                     NRHIEKILTQSPFTSLEQIREILSKQDYFEQINFRFKEWETEYKKIESQLKDLEKDTY
                     VVNFDIDNYEKIKEEVNNLSNIIEELSRKESVKQNQINEMKEHLDEKNRLMSESERLT
                     ARESRLNDLKNLFKANGFINYVSTIYLKNLVRAANRRFLELTGHQLQLQLNDKNEFVV
                     CDMMNDGHLRHIKSLSGGQTFLAAFSMALALADIINGNKPGIGNFFFIDEGFGSLDKN
                     SLRMVFQALSSLQKDNRIVGIISHVEDLQQEIPVSLSVFLDPQNGSQIIPSWLST"
     gene            complement(5929..7161)
                     /gene="sbcD"
                     /locus_tag="PLF20_01855"
     CDS             complement(5929..7161)
                     /gene="sbcD"
                     /locus_tag="PLF20_01855"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HOX79790.1"
                     /translation="MKIMHTSDWHLGKRLGEFSRLEEQKEVLDEICSISDLEKVQMVL
                     IAGDLFDSYNPSVEAMELFYQTLKRLSDEGKRAVIAIAGNHDMPERIEAPNPLAKECG
                     IILSGLPHSIASPFYLSSGLRITDSGPGWVQLKIPQVEVPVNLLLTPYANEFRMRTYL
                     GSENQDETLREILSNKWHRNLHEIANNQSVNILIAHLYMISNEGEPLEEPLDEEKPIL
                     TPGGASAVFTRQLPLDLHYVALGHLHRRHTLDGCAFPVVYCGSPLSYSFSEAFQNKSV
                     EIIEALPNLTTKHYSIGLTKGKRLVRKKFDSPEEAFQWLPHHTDCLLEMTLHTDMYLN
                     AEITNHFHRLHPGIVQIIPEFPEESNLKEKEEIAPDPTGEIMELFKDYFRSIKQVPPD
                     EETLDIFREIIANEGDNQ"
     gene            complement(7492..9777)
                     /locus_tag="PLF20_01860"
     CDS             complement(7492..9777)
                     /locus_tag="PLF20_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOX79791.1"
                     /translation="MESLYIIIVGFLFLLAIVDLIVGVGNDAVNFLNSAVGSKAAPFK
                     IILAVAFVGVVTGTLFSSGMMEIARSGIFNPSMFYFNEVMYIFLAVMITDIVMLDAFN
                     NLGLPTSTTVSLIFELLGASVAVAVMKTIIDPMAVSNVAEYLNTAKVLAIIAGILLSV
                     VIAFFFGTVIQYISRLLFTFRYKQKLPYLGGIWGGISFTIIIYFLILKGIRGAAFIDE
                     TTIHYIESHTATLMLLSIVGFTIIFQLLMWLFKINVLKIVVLTGTFALAMAFAGNDLV
                     NFIGVPVAGYESYRIYIQSGVTDPNSLFMGALAQPIKSPWYFLFIAGIIMGLTLIFSR
                     KARAVTNTEVSLSRQEEGLERFSPNPFSRNIVRMLIRFGNTTDKILPEGMKKFIASRL
                     TPPDAEEMKQAHKAGISYDLVRASVNLVVSSALIAYGTSHKLPLSTTYVTFMVAMGSS
                     FADGAWGRESAVYRVSGVMTVIAGWFLTAFIAFSVAFILATIIFFGKIPAIVILMILA
                     ALMLIHQQKVFVRKEKEAKIAQEILSDDSAISAEKMIARCNNDVLSILKTTDRLYTRI
                     LVGLFKEDRKKLKAVQAEVDEFNKRTKLLKSNVHNVVEKLKEDDVETGHFYVQVVDFM
                     REIAHCINYISEPALNHVENNHKGLLPSQKAELTRISAALSELIHTLQHMIETCDLEQ
                     RDKIVKLQQQVNEYIDKARKDQVKRIKNHETGTKNSLLYLNILAETKNLSLFILNLYK
                     SHRDFVVFSIGHEMQILSDDK"
     gene            10310..10549
                     /locus_tag="PLF20_01865"
     CDS             10310..10549
                     /locus_tag="PLF20_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOX79792.1"
                     /translation="MSDIENRVKAIIADKLNVDESEVTPEASFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAEKIGTVGEAIKFIEGNIKS"
     gene            10572..11825
                     /gene="fabF"
                     /locus_tag="PLF20_01870"
     CDS             10572..11825
                     /gene="fabF"
                     /locus_tag="PLF20_01870"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005645359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOX79793.1"
                     /translation="MGLRRVVISGLGALTPIGNDVNTLWASLVDGVSGAGPITRFNAE
                     KFKTKFACEVKGYDPLNFFDKKEVRKYDLFSQFGIVAADEAINDSGFKQGNYNPERVG
                     VIWASGIGGIMTLTEEIRAYVTGDGTPRFSPFFIPKMISDITSGHIAIKHGFMGPNFS
                     TVSACASSANAIVDAFNNIQRGKADIIITGGSEASISEAGIGGFNAMHALSTLNDDPR
                     AASRPFDKDRDGFVVAEGAAALVLEDMEHALARGARIYCEIAGAGLSADAYHITSPHP
                     EGAGAAIAMRLAIEDAKLQPSDLDHINTHGTSTPQGDIAEVKAIMKVFGEHVYNLNIN
                     STKSMTGHLLGAAGAIEALATVLAVYYDIVPPTINHFTDDPEINPALNFTFHKAQQRT
                     VNAAISNTFGFGGHNASILMQKFFK"
     gene            11893..12573
                     /gene="rnc"
                     /locus_tag="PLF20_01875"
     CDS             11893..12573
                     /gene="rnc"
                     /locus_tag="PLF20_01875"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOX79794.1"
                     /translation="MKNIFGFWPENIFLYKLALRHKSAALEFVNGERLSNERLEFLGD
                     AILDAVVAHYLFQLFPLRDEGFLTQMRSKIVNRQQLNALSHHLGLDNYIVASREINHV
                     RSINGNALEALIGALFLDKGFKFAEKVIINRILKIHFDLDELQNKDFNFKSKLIEWGQ
                     KEKHQVNFLLRGETGKGMNKQYLVEVLIDQKKISEGRDFSIKGAEQIAAEKAILRMKE
                     IFSTSSFN"
     gene            12634..13119
                     /locus_tag="PLF20_01880"
     CDS             12634..13119
                     /locus_tag="PLF20_01880"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HOX79795.1"
                     /translation="MPKTLIESELNENYRIYSLSTDLPDYKLCYSLNRQLKWDFRRRS
                     HLHLTFVKKNSPRQFSLFQYELDQFARFFLLYPFSEQIIVLPNCFFILQGCIHLSTEQ
                     KILETIRWIDGIHSFYFIDIQNPNASSAIHDFFDSLIYDLEYHLLQLDVSRAANAMIR
                     Y"
     gene            complement(13541..14848)
                     /locus_tag="PLF20_01885"
     CDS             complement(13541..14848)
                     /locus_tag="PLF20_01885"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOX79796.1"
                     /translation="MKSLLYITYYWPPSGGPGVQRSLKFTRYLPQFGYIPSVITVDPK
                     WAYYPLLDPSLEKEIPDYIKVYKTKTREPFRFYNITASQNKLPKPGFAGEGKPGLSQV
                     LARTLRGNLFIPDPRKGWNKFLIAQALELIKQDIPAAIVTSSPPHSTQLAGLKLKQRT
                     KIPWMADLRDPWTDIYYYDEFRHLPWIKHKDAQLELSVLENADAILVVSPSIKNLFLS
                     KSAKISEDKIHVIPNGFDDEDFNSISNQPKNESFTITYTGTLSTAYNIDALIHAVKHL
                     KNSNPNIKIHIQFIGKISPEILQSLKNNGLENMLSIIDYLPHREAITQMMAADVLLLA
                     IPKINENKGILTGKLFEYMATGNPILGIGPPDGDASKILDETHSGIMFDYDQNNEVLS
                     FLRSHYEIWQKGDNLRRSSPKVEKYSRKALTAQLAQIIDQFAR"
     gene            complement(14857..17316)
                     /locus_tag="PLF20_01890"
     CDS             complement(14857..17316)
                     /locus_tag="PLF20_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79797.1"
                     /translation="MKKTLFQNLMPYIAAIIIFLLATFLLFTPLFQGERLVQGDVTNF
                     KGMSKEIQDYREKTGEEPLWTNSMFGGMPAYQISVKYKNNLFSYVDKIFRLGLPRPAD
                     YVFLYFIGFFFLLVVMGVNPWIAIIGALAYGFSSYFIIILEAGHNTKAHAIAYMAPLL
                     GSILLTMKGKWLKGGILTAIFACLELYANHLQITYYFLILVLIVWIAFLIKSIREKQL
                     VSLGKATGVLIIAAFLAILPNFTQLWTTWEYSKETTRGKTELSHQKEIQTRGLDKEYV
                     TQWSYGVAETWSLMIPNVKGGETEQLGKSEFALEKIEPNMRRAIAQQNHYWGEQPFTS
                     GPVYVGAIIVFLFILGIFIVEGTLKWALVVATLLSIMLAWGKNFMPLTNFFLDYVPLY
                     DKFRAVSMILVIAELCMPLLGILALWKIYQYPSLAKNEASSFRKKFFIAYCLTAGISL
                     IFYMFPNLFFNFISSSERSQLNELRTQVADPSQIAVFIQSLETARIAIFRHDAIRSFL
                     FITIAAGFIYAYTSKLRFPRPLFLISMGLLIFIDMYVINKRYVTEDKFESSRIVDSPF
                     TPTPADQLILKDTDIHYRVLNLTTNTFNDARTSYFHKSIGGYHGAKLKRYQELIDHQI
                     SKNNMEVLNMLNAKYFIVPDENRKPSVRINMEALGNAWFPKEYKLVNNADEEMEALNH
                     FKAAETAIIDKRFAQYLEEYQSLPDSTAIIQLKEYAPNRLVYHSITNTDRLAVFSEIY
                     YPYGWQAYVDDIPQPHFRANYVLRAMIVPAGEHEIIFKFEPKSYYLGEKISLAGSLLL
                     LIMLIGMIGAEIKKKFIIKRG"
     gene            complement(17574..18719)
                     /locus_tag="PLF20_01895"
     CDS             complement(17574..18719)
                     /locus_tag="PLF20_01895"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOX79798.1"
                     /translation="MMTQSNRAGVYIQQPSGYNAFIPSPLPPNPPIQLEGELQTILSE
                     ADQALGRLDGSITIIPNPDLFVAMYVRKEAVLSSQIEGTQSSLQNLLEAEAEILSETA
                     PKDVAEVINYVKAMNYGLERLNELPLSVRLIREIHSILLQNVRGRHASPGELRQSQNW
                     IGPEGCTLREAVFVPPPPFYVPQALSDWEKFLHAQIDIPLLIKIGLAHAQFETIHPFL
                     DGNGRLGRLLITFILTEQEMLKKPVLYISYYFKQHKQKYYELLQNIRDTGDWESWLSF
                     FLRGITVVSNEAATTARNILNLRENHRAMITQHLGRMAANAFKVLDYLYIRPIITIND
                     IKEIIKTSYPAANELINRLVNIGIIQEITGFKRNKRYSYTSYIALFS"
     gene            complement(18877..21111)
                     /locus_tag="PLF20_01900"
     CDS             complement(18877..21111)
                     /locus_tag="PLF20_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOX79799.1"
                     /translation="MKNLKLKLVVSVILIASVLSGKAAIITPPDEGMWLPMFIERLNY
                     TDMQKLGLRLTPEELYSINHSSLKDAVVGLAAGSQPEGYFCTGEIVSDQGLLFTNHHC
                     GYNYIQEHSTLENDILTNGYWAMSFEEELPNPGLTASLLVRMENVTKAVLQNVTPEMD
                     EDAREQAISEVTDSIVKEASEGKKYNCTVKSFFNGNEYYLFVYQTYEDVRLVGAPPSS
                     VGKFGGDTDNWMWPRHTGDFSIFRIYTAPDGSPAPYSKENVPLKPKHFLPVSIKGVQK
                     GDFAMIWGFPGGTDRYMTSWEVKQAIDQTEPAIVKIRDLKLKIMKEDMQTDPRINLMY
                     AAKYAATSNYWKYYIGQIRGLKRLNVYEKKLKTEEEFLNWVAQDPQRKARYGEALELI
                     QTSLHNQQPYVLPITYVGEAVLQGPEIILWAMRSSNLPQLMEKIEKAKGTEKEELNKK
                     FQNTLEKLSKRAEDFYKDYNPSTDKKLFAQMLQLYYRDISPEMRADIFKDLEKKYKGD
                     FDKWSDDIYKKSIFADEKRFMAFLQNPSLKVLKKDPALEIGTEVRNHFILLYSQVENA
                     QKEEHKGSRLYIAGLREMNPGKLFAPDANSTLRMTYGSILDYYPADAVHYDLQTTLTG
                     VMEKEDPDNPEFIVDKKLKDLYEGKDFGRYGTQIHGRTDIVTCFLSNTDITGGNSGSP
                     VMNANGELIGLAFDGNWEAMSGDIAFEPNLQRTISVDIRYVLFIIDKLGGCHRLIDEL
                     KIVE"
     gene            21234..22343
                     /locus_tag="PLF20_01905"
     CDS             21234..22343
                     /locus_tag="PLF20_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOX79800.1"
                     /translation="MRYQNDTRIVMTLDGGGTNFVFSAMQAEKEIITPFAFPSQASTL
                     SETLYNIETGFRRIKSLLPGEPAAISFSFPGPADYELGIIGDLVNLSVFRGGVPLGPY
                     LEDIFKIPVFINNDGDLFAYGEAISGFLPEVNRWLADAGSPKRFQNLLGVTFGTGFGA
                     GIVLRGELFLGDNSAAGEINRMSNKLYRNMSVEESVSIRGVRRVFSNYSNIPFDQTPS
                     PKEIYEIAIGKRPGNQKAANKAFEELAIVAGNAIADALTLVDGLVVIGGGLSGAWPVF
                     LNCLVEELTLPFTTFSGQNIDRLEIKFFNLEDGAQRELFLKGSTRKIEIPGSARKITY
                     DPEHRSGVGITRLGTSRATALGAYNFALRKLDSMK"
     gene            complement(22387..22971)
                     /gene="nadD"
                     /locus_tag="PLF20_01910"
     CDS             complement(22387..22971)
                     /gene="nadD"
                     /locus_tag="PLF20_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009130834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOX79801.1"
                     /translation="MKNNNNKTGLFFGSFNPIHNGHLMIANYLVEYTDLKQIIFVVSP
                     RNPLKEKELLAEDRHRLRMVELAIQDDRRFSASDIEFHLPTPSYTVHTLEYLRQIYPQ
                     ERFVLVIGGDNLQYFDRWKDPELILEKHEIYVYCRLGFEGGKFANHTSVKIIASPLID
                     ISSSMIRQAYAQGKDLRYFQPLTVYEYIRKFNIY"
     gene            complement(22961..23548)
                     /locus_tag="PLF20_01915"
     CDS             complement(22961..23548)
                     /locus_tag="PLF20_01915"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005682289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HOX79802.1"
                     /translation="MNEAERCPWSLSDERMMKYHDEEWGVPLHDDIRLFEFLVLDIFQ
                     AGLSWRTILHKRENFRKAFHNFDFSKIASYNKKKTDELLLNSGIVRNRSKVEATIHNA
                     KCMLDLVEKRGSFDKYLWSYVDRKPIVNNYRTYNEIPGHTSLSDQISADLRKEGFIFV
                     GSTIIYSFLQASGVVNDHIITCFRHREIQKRHYEK"
     gene            23839..24165
                     /locus_tag="PLF20_01920"
     CDS             23839..24165
                     /locus_tag="PLF20_01920"
                     /inference="COORDINATES: protein motif:HMM:NF025652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4372 domain-containing protein"
                     /protein_id="HOX79803.1"
                     /translation="MGKYTTKNLVGQPVFKQVMKILPREPFDLVVRRCGSDRYYKSFF
                     SWDQLVVMLFGIFSRCDSMGEVCDGMRALAGKFNYLGMDSAPSKSTAGDALRDRDNEV
                     FQVVLF"
     gene            24306..25067
                     /locus_tag="PLF20_01925"
     CDS             24306..25067
                     /locus_tag="PLF20_01925"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOX79804.1"
                     /translation="MAKKGSLKVHMLTDVHADTAVFATISEAKMHDKKFLQHVNPSRG
                     SMLVFDKAYNYYRQFAEWTDEEVYFVCRLKDNAKAEVQEVLHETTLGKEEHGVYRVEH
                     IHLKYTKEKRERTLCLRLVYYRDEQGRSYKFITNSWNITAEEVALIYKYRWTIETVFK
                     KMKQNFQLHFFYSDTENGIKTQVWCTLIAHLLLNVIRLLSRSKKAFSTVAAFIRIHLI
                     SHLDMMWIVAEGRRTYTKHTASRNKSPATVQMSLF"
     gene            complement(25550..>25939)
                     /locus_tag="PLF20_01930"
     CDS             complement(25550..>25939)
                     /locus_tag="PLF20_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX79805.1"
                     /translation="TLYAIVETENQFLASVGQGANAWLLVVDSLGCEAPGVCWVGQEE
                     ISAPPQETQLEVFPNPAGEEVWVKSHGHLAGIEIYAMNGQKMSLPALNAENPLRIDTR
                     TWPAGLYMVRATLQNGRVLMQKVMKCR"
BASE COUNT         7644 a         4913 c         5205 g         8177 t
ORIGIN      
        1 aattaattag ttatcaaaaa gttattaaca atttgagttt atagacagac tctaagtaat
       61 tttgaggtcg agacttttcg gagtggactc aagtttataa agtaaaaaac gaagaaaggg
      121 agaaaaggag aatggataat ggtggctagc gaatggtgat tagtgaatgg tgattgaagt
      181 ttgttcgtaa taaggtgttt gttgtaaggt gtttgttgct tgttttaggt tgtaagttgt
      241 aaatcgtaag ttgttggtta tcagttgtaa gttgttcgtt gtttataatt tatttgagaa
      301 aataataatt gatctattgc gtaatgagaa gttttttagt cagaataata ttttcggaaa
      361 ataattgaat gatgtatata ccacttttta attttactgg tagttgaaaa atataaggag
      421 ggctattgaa ggtgttgctt agcatttcgt gtcctgtaag gctataaata tgaatggatt
      481 ttaatttaag gggaatggag gagtttttaa tttgaatttg atttagaaat ggaatataac
      541 tcatttcgat gatggacatg ttattttcag atggtactgc agccgaaggg catgaccagc
      601 tcattgcata attggcttgt ggatgtttgc cagtagcctc aaatgtaata taaaaaccat
      661 agtcgctgat tatttcagtg gaatcgttgc caggccatat tgtttccaag ggtgttccaa
      721 gaggttgttg cctatcgaag atagtaattt ttgagttctg aagattgagg tcgaaaaatt
      781 tcagcttata gggtccaggg taatttggga gaatactcat attttgagtg ataaaaattg
      841 atggattcat taaaaagcca cccgagtcga atattgtatc ctgacaaagc atacctccgt
      901 tttcagagaa ggcttgttgg atagcatccc ataattcgtc gtagcccatg tcatatccca
      961 gagcccagat gccgattcct tttaggtcgg aagcaattac tctttggtat ttatataaca
     1021 agcttggggc ctcatcaata aaacagtgat tccagccatt ggcttggtag gcatagtaaa
     1081 ctgagcgtga gggaaaattc atttttcggt tactccatga gaaaaataca ggattatttc
     1141 taacattgcg ataagtaact gcacttccac ttcctcttgt tgaagcagga gcttctaact
     1201 gggtcacagg ccatattctg ccataatatg gcactcccag caaaatcttt tctttcccaa
     1261 ttccttgtga aaaataatat tcaatggttt gactaacatt atatggataa gtagtttcca
     1321 taggatagag aggagaaacg gggccagccg ttgtgcttaa attccagtaa taatcataag
     1381 ccattaccat aaaaaaatcg acatcttgtg ccagattact taactcaaaa gtattgttcc
     1441 agttgactgc aggagtggcc atgcttaaaa ggcaatcagg agcagtagaa tcgagatatt
     1501 ggcgcagttc atgaatgtaa tttgttaagg aactggcttg tgaagatggt acagcctcaa
     1561 aatcaatgtt tactccatga attcctcttg actttacaag atgaacgatt tgctcgttca
     1621 aggttttacg cgccaaagca tcttcaaaaa aactggcatg cccgctaaaa agcgttacac
     1681 ataagtgtac atgaattcct tttgtcaaag ccgtgtcgat tactgttgca tttccgaaat
     1741 cattagtggt aaccggcatc ccactgcttg catcgacttc ataagaaaag taacataaat
     1801 cggtaatcaa atcccatcga taatcacccc acgaattatt catccaataa gggtgatatc
     1861 caaaaatcat ctttcctttt ttgagaggaa tgttggtttt atttctgccg ggcttaccat
     1921 gagaagtatc agaaggaata ggatgtactt tataatattg ttgttcaata acatgaatag
     1981 atcgaaattc tgtatcttga gcaagcaata cgaacgggaa aagcacaagg aaaataaggc
     2041 aaattactgt cgataaaaaa tggtaaaata atttcatttt aaaaaatttt caagaaaaaa
     2101 ttcatataga ttattcaggg ttttcttttg cataagcaat caatttgttg taccattctt
     2161 ctccgtagcg ttgaatgaga ggttttttca agaaaacata cagaggaata cccagagatt
     2221 tgcctttttt tagggctggc ttacaaatat tccatttatg ataaatgagg aaatctcccg
     2281 taactgatct tccgactcga ataggataga gaaaacagga ctgaggtttt tggaagtttg
     2341 ttttgccggc ctgaaatgct aattcgaaag cacaaactgc agttccattc tcgaaattta
     2401 taaaagcgca atctccatca tcaatcaaag gagtaaccaa ttgaccactt tgatcgaatt
     2461 caaaaatgcc tgactttttt attgtggcca ctcctttttc ggtcatatac ggaattactt
     2521 gttccagaca attttctatc aagttaattt cgtctgcaaa taaaggagcg cccgcatctc
     2581 cctcgatgca gcatgcacca tggcatctgg taagatcgca agtaaatcga catcgaataa
     2641 tttcgtcctg caccataatg ccatcaataa taatcatgat acaacaagaa tcaatggttt
     2701 aaattataga ttcagttgag gatttttcga atgtatttgt ttgtttaatt tttttgaact
     2761 aagaaaaaat aatgtattgg gatataaaca tgcaaatata aaatgttcat cgaaaaaaag
     2821 gacaagaatg ataggaaatg ataccaattt atgtcgataa ccatgaaggg ataatctggc
     2881 tgccattttg tggatcgagg aaaacggata ggcttacagg aatttcttgt tgtaaatctt
     2941 ctacatgaga gataattccc acaattcgat tgtctttttg gagagacgac agagcttgaa
     3001 agaccattcg cagtgaattt ttatcgagcg aaccaaaacc ttcgtcaata aaaaagaagt
     3061 tacctatgcc aggtttatta ccgttgataa tatcggccag tgcaagagcc atagaaaatg
     3121 cagccaagaa tgtctgtcca cccgataatg atttgatatg acgcaggtgc ccatcattca
     3181 tcatatcgca aacaacgaat tcattcttgt cattgagctg taactgaagc tggtggccgg
     3241 ttaactctaa gaagcgccta tttgcggctc tcacaagatt cttaaggtag atagtggaaa
     3301 cataatttat aaaaccattg gctttgaaca gatttttcaa atcattcaaa cgagattctc
     3361 tggccgttaa tcgctctgat tccgacatca ggcgattttt ttcatcgaga tgctctttca
     3421 tttcatttat ctgattctgt ttgaccgatt cctttctcga gagttcctct ataatatttg
     3481 acaaattgtt tacttcttct ttaatttttt cataattatc tatatcaaaa tttactacgt
     3541 aagtatcctt ttctaaatcc ttcaattgcg attctatctt tttatactcg gtttcccatt
     3601 ctttgaatcg gaaatttatc tgctcgaaat aatcttgctt gctcagaatt tccctaatct
     3661 gttccagaga agtaaagggc gattgggtca gaattttttc gatatggcga ttttgaacat
     3721 tcaatttttc tttggcttct tcgaggtcta tatgctttga ttttagttcg gcatttactt
     3781 ggtcccaatg gtgttgtatt ttttcggcct gttcttctat ttgttgcagt tctttcttta
     3841 cagatttaat ttcttcaagg gttgttttga taatattttg aagctcatcg tcagagtgga
     3901 tgaagttttc ttcccattcc ctgcctccat atttattttt caacatttcg gcctgtcccg
     3961 aaagaaaatt tatttgctgc tgcaattgat tcatttcagt gctttgtttt ctatgttttt
     4021 cttctaagtt atgaatttct tgttctaatg tatttcgatt caagttaagc tttctcagcc
     4081 gattttgtaa aaattctatt ttttctgaaa attcctgata agccttttta ttaccaactt
     4141 ctaagccatc gaaatgataa gtgttgaaat gtaaatcaat ttgttgagtt aatttttcta
     4201 accctcggtt agccttttct atttcatctg attttgcttt cagcttgcca aggtattgtt
     4261 tcagttcact catagcatcg ataatggctt gttgttgagc ttttagctgt atttctgtgc
     4321 tttcgagttt cttcaatttt gcttcgacct caggtgcgct aaaaagatgt ggatgtgagg
     4381 tagacccgca gagtggacaa ggcattcctt cccgaagttc ttttgcaaaa tcggctaatt
     4441 tagtggttac ttttaaggat tgaatctgat tcctaatttc atctaattga ttcttagata
     4501 aattatagtt tctctgtatt tcttccaaaa cctctggaat agatttttga gccaataact
     4561 cgtggcataa aatgagatga gaattttgca gggcatcttg ttttatgatt tccatgtcgt
     4621 tgagcgccga ttgctttcgt acctctgttt gctgtttggc ttccaaaagt ctatcatatt
     4681 ctgtgaacca ttgatttatt tgtttgattt cattggggtt gggaagttgg gtttcgagtt
     4741 cttcatattc tttgagaatg gaggattttt cttctttttt cgacttgagt gtttcatcat
     4801 gcgatttgat ttgatctatt aaagcttgaa tcttaggatt gatttcaata atctgctgtt
     4861 gaatttttcg ataatgcaaa agttcttcta tcttttcaac aaaaactttg cggaatggaa
     4921 gttcttccga tttctgtttc cataaagcaa gctgattttg gatattattc ctttgaggtt
     4981 ggagtatctc tatttgatga gaaagctctt tgcatctttt ttcaagtgaa ttcaccctat
     5041 tcgatatctc gtcgagcaag tccaaagggc tgcggaagga ggattcagcc atttgatatt
     5101 tttcgatgaa attacggttt ctctccataa cctcggcttc cttgttcagc tgctcgaatt
     5161 gaagtttcaa accttgccat tgttgatttt tttcgtaaag aagcgcctgc tgatcttttc
     5221 ttttttgaag ctcagaatat ttttgtcgtt cttgcaatag tgccgcagta atcgtgttta
     5281 attcgtcctg aagagtggta attgcttcgg gagtggcgct taagctttcg aggcgagtgt
     5341 taacctgaat caggatttcg tggtttttct cgaataagtt tttggccttg ttgtagagat
     5401 caaattgttc gagctgaaag atttctttta gcattcttat tcggtcggcg ccgccaagct
     5461 gaaggaattc ctgaaatttt ccctgaggaa taataatagt acgacggaaa ttatcgtaag
     5521 aaagaccgat tacctcatca attttttcta aagcaatggg gagccaagta ttgttggatt
     5581 tttggtaagc cttgcgatcg agagattctg tttcgtgata attttttggc tttcgccggc
     5641 cttccacagt tatcctgtat tgttgggaag ggttcccggc attaaagatg aagtcaatcc
     5701 acagacggtt cgaacgcaga ttcatcatgt tgtaattacg ggcttcgcgt gaattcaatc
     5761 gttctatatc gccgaaaagg gcatataata tggcttcggt tatgctcgat tttccgcttc
     5821 ctactggtcc aaaaatacca aacagtctgg cttcaaccaa tggcctgaaa ttgatctcct
     5881 gcctctgctg ataactgaat aaaccctcta aagttaattt tactggaatc attgattatc
     5941 cccctcgttt gcaataattt cacgaaatat gtctaaagtt tcttcgtcgg gcggcacctg
     6001 tttgatgctt cgaaaataat ctttgaataa ttccattatc tctccagttg gatcgggagc
     6061 aatttcttcc ttttctttca gattggattc ttcaggaaat tcaggaatga tctgaactat
     6121 tccgggatgg agtcgatgaa aatgatttgt gatttctgcg tttaaataca tatcggtatg
     6181 tagagtcatc tcgagaaggc aatccgtatg atgaggaagc cattgaaaag cttcttcggg
     6241 tgaatcaaac ttcttgcgaa caagacgttt ccccttggtc aaaccaatgg aataatgttt
     6301 tgtagttaga ttgggtaagg cctcgataat ttcgaccgat ttattttgaa atgcttcgct
     6361 gaagctataa gacaaagggc tgccgcaata aactactggg aaagcacatc catcgagtgt
     6421 gtggcggcga tgaagatggc ccaaggcaac ataatgaagg tcgaggggca gttgtctggt
     6481 aaagactgca gaagctccac caggtgttaa tatgggtttt tcttcatcca agggttcttc
     6541 gagtggttcg ccctcattgg atatcatgta cagatgggct attagaatat tcacactctg
     6601 attgtttgct atttcatgaa ggtttctgtg ccatttgttg gataagattt ctcgtagggt
     6661 ttcgtcttga ttttcggaac ccagataggt tctcattcgg aattcattgg catagggagt
     6721 aagaagtaaa ttaacaggaa cttcgacttg tggaattttg agttgcaccc agcccgggcc
     6781 cgaatcagta attcgtaatc ccgaactcag ataaaaaggg gaggcaatgg aatgaggcaa
     6841 acccgataaa atgattccac actctttggc aagaggatta ggagcttcga tacgttctgg
     6901 catgtcgtga ttgccggcaa tagctataac ggctcttttc ccctcatccg agaggcgttt
     6961 caaagtctgg taaaacaact ccatcgcctc gaccgaggga ttataagaat cgaataaatc
     7021 tccagcaatc aaaaccattt gtactttctc taaatcggat atactacaaa tttcgtcgag
     7081 aacctctttt tgttcttcaa gacgactaaa ttctcccagt cgtttgccta aatgccaatc
     7141 agaagtatgc ataattttca tatcgggtgg cagtttgttt tttattcgaa gatattaaaa
     7201 atcaaataga tatcatgatt caatagaata cacgcagaac acaaaattat gcattagtaa
     7261 acaatatttt gaccctgctc ttatagccca tgcctcgcta tctcatcttt ccgaaaaact
     7321 taaaataacc caaactatta tggttttttc acttcattgc gcaatctcta atcgccattc
     7381 tatatcactg ctgtcgcact acgtattcct ccatcacgtt cattttaata acttatatac
     7441 gtttaattca ctattctccg ttctggggaa tgcataacag gaaacatgtt cctatttatc
     7501 atcacttaaa atttgcattt catgtccaat agaaaaaacc acaaaatctc tatgcgactt
     7561 ataaagattg aggatgaaaa gtgacaggtt ttttgtttcg gctaagatgt tgagatacaa
     7621 aaggctattc ttagttcctg tttcatgatt ttttatgcgt tttacctgat ctttgcgggc
     7681 tttgtcgata tattcattta cttgctgctg aagttttact atcttgtcgc gttgttcgag
     7741 gtcgcaagtt tctatcatgt gctgcaatgt atggataagc tctgaaaggg cagccgaaat
     7801 tcgtgtcaat tcggcttttt gtgaagggag gaggcctttg tggttgtttt cgacatgatt
     7861 gagtgcgggt tcggagatat aattgatgca atgtgcaatt tcacgcataa aatctaccac
     7921 ctgtacgtag aaatgtccgg tttccacatc gtcttcctta agtttttcca ctacattatg
     7981 aacattactt ttcagaagct ttgttcgttt attgaattcg tcaacttcag cttgcacggc
     8041 ttttagtttt ttacggtctt ctttgaataa acccaccaga attcgagtgt ataagcggtc
     8101 ggtagtttta agaatcgaaa gaacatcgtt gttgcagcga gcaatcattt tctcggctga
     8161 aatagcggaa tcatccgaaa gaatttcttg agctattttt gcttcctttt ccttgcgaac
     8221 gaagactttt tgctgatgaa ttaacatcaa agcagcgaga atcatcagaa taacaatagc
     8281 aggaatttta ccaaagaaga taatagttgc taagataaaa gccacgctaa atgcaataaa
     8341 tgccgtgagg aaccaacctg caataacagt cataacgccc gaaaccctgt aaaccgcact
     8401 ttcccgaccc catgctccgt ctgcaaacga cgaccccatg gctaccataa atgtaacata
     8461 agtagtgctc aagggaagtt tatgggaagt gccataagca atcaaggcac tcgaaacaac
     8521 taaatttacc gaggctctca caaggtcgta agaaattcct gccttgtgtg cctgtttcat
     8581 ttcttcggca tcgggaggag taagtcgtga ggcaataaat ttcttcattc cctctggaag
     8641 tatcttatct gtcgtattgc caaatcggat gagcatcctc acaatatttc tcgaaaaagg
     8701 gttaggagaa aatctttcca acccttcttc ttgcctgctc aaacttactt cggtgttggt
     8761 tacagctcgt gccttacgag aaaaaatgag agtcaaaccc atgataatac cagcaataaa
     8821 taaaaaatac catgggctct tgataggttg agctaaagcc cccataaata gagaattagg
     8881 atcggtaacc cctgattgaa tatatattct atacgattcg tagccagcca caggaacacc
     8941 aatgaaattg accagatcat tccccgcaaa agccatggca agtgcgaaag tgcctgttaa
     9001 aacaacaatc ttaagaacat tgattttaaa aagccacatc aacaactgaa aaattattgt
     9061 gaaacctact atgcttaaaa gcattaaggt agcagtatga gattctatgt aatgaatagt
     9121 agtttcatca ataaaagccg ctccacgaat acctttcaat attaaaaaat aaatgatgat
     9181 ggtaaatgaa attcctcccc agattcctcc taagtaaggc agcttttgct tataacggaa
     9241 agtaaaaagc aaacggctta tatattgaat gactgttcca aagaagaatg ctattactac
     9301 cgataataat atacctgcaa taatcgccag aaccttggca gtattgaggt attctgccac
     9361 attagagact gccataggat cgattatggt tttcatgacc gcaactgcta ctgatgcacc
     9421 taaaagttca aaaattaatg aaacagtagt tgaagtgggc aatcctaagt tattgaaagc
     9481 atccagcatt acaatgtcgg tgatcataac tgccagaaaa atgtacatca cctcattaaa
     9541 ataaaacatg gaaggattga aaattccact acgcgctatt tccatcatcc cactcgaaaa
     9601 tagagttcct gtaacaactc caacaaaagc caccgccaaa attatcttaa agggcgctgc
     9661 ctttgaacct actgccgaat tgagaaaatt tacggcatca ttccccactc caacaataag
     9721 gtccacaatg gctaatagaa acaaaaaccc gactatgata atatatagac tttccatcaa
     9781 atgattaatt tggccacaaa attatattac tcatttgttc tgtagattat ttccatttta
     9841 agaaattgtt aatgtaatcc attaaaatac tatcgacgtg aaatgataga agaaagattg
     9901 gaaaacaaag aattcttttg caattgatag ggtaaaatga gattcgatcg tgttgacctc
     9961 caaccaaata aatagtctta taactcactc cttcaacaag atgataaaca atcaaataga
    10021 agatcaatcc gaaacacttc atattttcag gccggctttt ttaaacttga agtttattat
    10081 ttgggaggaa gattttatgg ataaaacaag ccgaataacg ttcagattgt gaaataaaag
    10141 gagtaaaatg agtggaaaca tcattcattt taaccaaatg gctaaactta tcaatatatt
    10201 gtatttcaat ttattgaatc aaattttagg ttttattaac atttaaaatt tgcacaattt
    10261 gtaaaatcgt attttctttg caaccttgtt taaccaaaaa accacactta tgtcagacat
    10321 tgaaaacaga gtaaaagcta tcattgctga taagctaaat gttgacgaaa gtgaagtaac
    10381 ccccgaggcc agcttcacca atgatttagg ggccgactcc ctcgataccg tggaactcat
    10441 tatggaattt gaaaaagaat tcaatattgc cattcctgac gaccaggctg agaaaatagg
    10501 cactgtaggc gaggcaataa aattcatcga aggaaacata aagagctgag gaacctattt
    10561 aacgttcaat gatgggatta agacgagttg ttattagcgg gttaggcgct ctgactccga
    10621 tagggaatga tgtaaatacc ctatgggcat cattggttga cggtgtcagt ggcgctggcc
    10681 ctattacaag attcaatgcg gaaaagttca aaacgaaatt tgcgtgtgaa gtcaagggct
    10741 atgatcctct gaattttttc gacaagaaag aagttcgcaa atatgacttg ttttcgcaat
    10801 ttggtattgt agctgccgat gaagctatca atgattctgg tttcaagcaa gggaattata
    10861 atcccgagag ggttggggtg atctgggctt caggtatagg tggcattatg accttaacgg
    10921 aagaaatcag ggcttatgtt acaggggatg gaactccccg ttttagtcca ttttttattc
    10981 cgaagatgat atccgacatt acctcagggc atattgccat aaagcatgga tttatgggac
    11041 cgaatttttc tactgtttca gcctgtgctt catctgcgaa tgccatagta gatgctttca
    11101 acaacattca aagaggaaag gccgatatca ttattacagg tgggtcggag gcttccataa
    11161 gcgaagcagg aataggtggg tttaatgcca tgcacgcact ttctactctt aatgatgatc
    11221 ctcgagcggc ttcgagacct ttcgacaaag acagagatgg ttttgtagtt gcagaaggtg
    11281 ctgcagcact cgttctcgaa gatatggaac atgccctagc cagaggagcc aggatttatt
    11341 gtgagatagc tggagctggg ctatcggccg acgcttacca tattacttct cctcatcccg
    11401 agggagcagg agctgcaatt gccatgagat tagccattga agatgcaaaa ctgcaacctt
    11461 ccgacctaga tcatatcaat acccatggta cttctactcc tcaaggtgat atagccgagg
    11521 tgaaagccat aatgaaagtt ttcggtgaac atgtttacaa cctcaatatc aactcgacca
    11581 aatcaatgac cggccatttg cttggagccg ccggagccat cgaagcactt gctacggtat
    11641 tggctgttta ttatgacatt gtgccgccta ccatcaacca ttttactgac gatcccgaaa
    11701 tcaacccggc tcttaatttt acctttcata aggcccaaca aagaactgtg aatgctgcta
    11761 tcagcaatac tttcggattt ggcggtcata atgcctccat cctcatgcag aaattcttca
    11821 aatgatggaa tgagaaaaat ttggttccgc attaggattc atttctccaa cgataaaaaa
    11881 ttttacatcg ctttgaaaaa tattttcggg ttctggcccg aaaatatttt tttatataaa
    11941 ctggccttga ggcacaaatc ggccgccctt gagtttgtta atggtgaacg cctcagtaac
    12001 gaacgactcg aatttttggg cgatgccatc ctcgacgccg ttgtggctca ctatcttttc
    12061 caactgttcc ctttacgtga tgagggcttc cttactcaaa tgcgtagcaa aatagttaat
    12121 cgccagcagc tcaatgcact ttctcatcat cttggcctcg acaattatat cgtggccagc
    12181 cgcgaaatta atcatgtcag aagcatcaat ggaaatgcct tagaagcact tataggggca
    12241 cttttcctcg ataaaggatt caaatttgcc gaaaaggtca ttatcaaccg aatcttaaaa
    12301 atacatttcg atctcgatga acttcaaaac aaggatttta atttcaagag caaactcatc
    12361 gagtgggggc aaaaagagaa acatcaggtg aattttcttt taagaggaga aacgggcaaa
    12421 ggtatgaaca agcaatacct tgtagaagta ttgattgatc agaaaaaaat tagcgaaggt
    12481 cgcgactttt ccattaaggg agccgaacaa attgccgccg aaaaagctat ccttcggatg
    12541 aaggaaatct tttcaacctc ctcatttaac tgattaaatt cttttattag gcaaaactat
    12601 taattttggg cttgctcctc tttttgcttc atcatgccta aaaccttaat cgaatcagaa
    12661 cttaacgaaa attataggat atattctctg agcactgatc tcccggatta taagctgtgt
    12721 tatagcttaa accgtcaatt gaaatgggat ttcagaagac gcagccatct gcatctgacc
    12781 tttgttaaaa aaaattcacc acgtcagttt tctctttttc aatatgaact cgatcagttt
    12841 gctcggtttt tcttgcttta cccattctcg gaacaaataa ttgtgctgcc caattgtttc
    12901 tttattcttc aaggatgcat tcacctcagc acggagcaaa aaatattgga aaccatccgc
    12961 tggatagatg gaattcattc cttttacttt attgatattc aaaaccccaa tgcctcttcg
    13021 gccatccatg attttttcga ctcattgatt tatgatctcg aatatcattt acttcagctc
    13081 gatgtgtcta gggcggccaa tgcaatgatt cgctattaga agtacccttt ttgatgaata
    13141 atgacgaagg gaatgatggt tgattcaaca atgttgctaa gaatatatga ttgattatta
    13201 atgtaataag gtgaggatta gaaattaaca tgggatttct tggtttgggt taaagtattg
    13261 taagctgaat tcatgttgac atattttaat tcgtcgaaaa cctaatggtg gctcagatat
    13321 aggaatgttg cgagtcgtgt aatatacaag aatgaatcta tcaaggtatt ggaatagaat
    13381 agcggttaca ggaatctgac cgcttgaatt ggaagaacag aatgcaggtt gcttttggaa
    13441 ttggctaaat tgaatcgaaa ggatttctct ggattcttat aggtggctaa ttatctatat
    13501 caattcgaat caggatgatg atatagaaca aggaaaattt ttatcgagcg aattgatcta
    13561 tgatttgggc taattgagcg gtcaatgctt tgcgtgaata tttttcgact ttaggggagc
    13621 tccttcgaag gttatcccct ttttgccaga tctcataatg actgcgtaaa aatgataaca
    13681 cttcgttgtt ctggtcataa tcgaacatga ttcccgaatg ggtttcatcg agtatttttg
    13741 aggcatctcc gtcaggaggc cctatgccca gaatcgggtt tccagtagcc atgtactcga
    13801 aaagttttcc tgtcagaatt cctttatttt cgttgatttt tggtattgcc aagagcaaga
    13861 cgtcagcagc catcatttga gtaatggctt cacggtgagg aagataatcg attatggaca
    13921 acatattttc caaaccattg tttttcaaag attgcagaat ttcgggagaa atttttccaa
    13981 tgaattggat atgaatttta atatttgggt tgctgttttt gagatgcttt acggcatgaa
    14041 taagagcatc tatattataa gctgttgaca gagtcccggt ataggtaatt gtaaaagatt
    14101 cgttctttgg ctgattggaa atactgttaa aatcttcatc atcgaagccg ttgggaatga
    14161 cgtgaatttt atcctctgag attttggctg atttggaaag gaaaagattt tttatggatg
    14221 ggctcaccac gaggatggca tcggcgtttt cgagaaccga aagttcgagt tgggcatctt
    14281 tgtgttttat ccacggaaga tgtcggaatt cgtcgtaata gtatatatct gtccatggat
    14341 cacgaagatc ggccatccat ggaatttttg tgcgttgttt gagcttgagg cctgcaagtt
    14401 gagtagaatg cggaggcgag gaggtaacaa tagcggctgg gatatcttgt tttatcagtt
    14461 cgagtgcttg tgcaataaga aatttattcc aaccttttcg agggtcagga ataaaaaggt
    14521 ttccacgtaa tgtacgagcc aagacctgag aaagccccgg tttcccttct ccggcaaaac
    14581 ctggcttagg gagtttattt tggcttgccg tgatgttgta aaatcggaat ggctcgcggg
    14641 tttttgtctt gtaaacttta atataatctg gaatttcttt ttcaagagat gggtctaaca
    14701 aaggataata agcccatttt ggatcgacgg taataacaga ggggatataa ccgaactgag
    14761 gaagatatct tgtaaatttc agagagcgct gaacgcccgg acctccgctg ggaggccagt
    14821 agtaagtgat ataaagcaat gacttcatag aagggtttag cctcgtttaa ttatgaattt
    14881 ctttttaatt tcggccccta tcatacctat taacattatc aaaagaagca gcgagcccgc
    14941 aagagatatt ttttctccca gataatacga tttgggttcg aacttgaaga tgatttcatg
    15001 ttctcctgca ggaacaatca tggctctcag aacataatta gcccgaaagt gcggctgagg
    15061 tatatcgtcc acataagcct gccagccata aggataatat atttcgctga aaaccgctaa
    15121 gcgatcggta tttgttattg aatgataaac cagccgattg ggtgcatatt ctttgagttg
    15181 gattatggcc gtggaatcgg gtaaagattg gtattcttcg agatattgtg caaaacgttt
    15241 atcgatgatg gcagtttcgg cagccttgaa atggttaagc gcttccattt cttcatcggc
    15301 attatttact aatttgtatt ctttggggaa ccaagcattt ccaagtgctt ccatgttgat
    15361 tcgaacgctt ggtttgcgat tttcatcagg gacgataaag tattttgcat taagcatatt
    15421 gagaacttcc atgttgtttt tgcttatctg atgatcgata agctcctgat accgttttaa
    15481 ttttgctcca tgatatccac ctatggattt atggaaataa gaggttcgtg catcattgaa
    15541 cgtgttggtt gtaagattca gtactcggta atgaatgtcg gtatctttca ggatgagttg
    15601 atcggcaggg gtaggggtaa agggggaatc tactatgcgt gaactttcga acttatcttc
    15661 ggttacataa cgcttattga ttacatacat atcaatgaaa atgagtaagc ccattgaaat
    15721 aaggaaaaga ggacgaggga atcgcaattt agaagtatag gcataaataa atcctgctgc
    15781 tatggtaatg aaaaggaagc tgcgtatggc atcatgtctg aaaatagcta tgcgggctgt
    15841 ctcgagagat tgaatgaata ctgcgatttg tgaaggatcg gctacttgtg tacgaagttc
    15901 attcaactgg cttctttcgg atgaagagat gaaattaaag aaaagattag ggaacatata
    15961 gaatattagc gagatgcctg ctgttaaaca ataggctata aaaaattttt tacgaaacga
    16021 tgaggcttca tttttggcaa gagaaggata ttggtatatt ttccacaatg caagaattcc
    16081 gagcaagggc atgcataact cagcaataac gagtatcatg cttactgccc gaaacttatc
    16141 gtaaagtgga acataatcga ggaagaaatt ggtaagtggc ataaagtttt taccccaggc
    16201 taacattatt gaaagtaagg ttgccaccac taatgcccat tttagtgttc cttccacaat
    16261 aaaaattcct aaaatgaata gaaatacgat gatagcccct acataaactg gacctgaagt
    16321 aaatggttgt tctccccaat agtgattctg ctgtgcaatg gctcttctca tgttgggttc
    16381 tatcttttcg agagcaaact cgcttttccc aagttgttcg gtttctcctc cttttacatt
    16441 gggtatcatc aacgaccatg tctctgcaac cccataactc cactgcgtaa catattcctt
    16501 atcgagacct cgggtttgaa tttctttttg atgactcaac tctgtctttc ctctggtggt
    16561 ttccttgcta tattcccatg tcgtccataa ttgagtgaaa ttgggtaaaa tagctaaaaa
    16621 tgcagctata ataagaactc ctgttgcctt acccaatgaa accaattgtt tttctctgat
    16681 agattttatc aaaaatgcaa tccaaacaat taaaacaagt atgagaaaat aataagtgat
    16741 ttgcaagtgg ttggcgtaaa gttccaaaca tgcgaaaatg gctgttaaaa ttcctccttt
    16801 gagccatttg cctttcatgg taagcaagat acttcctaag agtggcgcca tataagcaat
    16861 ggcatgcgct ttcgtattgt gcccggcttc taaaataatg atgaaatatg atgaaaaacc
    16921 ataagctaat gcccctataa tggctatcca tgggttgact cccatcacaa ccaagagaaa
    16981 gaaaaatccg atgaaataga gaaaaacata atcggcagga cgcggcagcc ctaaacgaaa
    17041 tattttatca acatacgaaa ataaattgtt tttgtatttc accgaaatct gataagcagg
    17101 catcccacca aacatgctgt tagtccataa aggttcttca cctgtctttt cgcgataatc
    17161 ctgtatctct ttcgacattc ccttaaaatt ggtgacgtcg ccttgtacca aacgttcacc
    17221 ttgaaacaga ggggtgaaaa gtaaaaaagt ggctaacaaa aaaatgatga tggccgcaat
    17281 atatggcatc aaattttgga acagggtctt tttcatgtgc agaatttttt acttggcaaa
    17341 tttgagttac tcgcaattaa ttcatgcaaa aataacaaat cagacgactt gtgcaaaaat
    17401 gtttctcctc catgccagat cagcattctc taattaatga ggggagaaat aagaagttat
    17461 gaacttaatt tgatataaaa ttcttcaaca actgattgtt ctgagcaaaa tttgaaaaaa
    17521 cttcttgtgg tgaaccattt tgttataaga tacagatttt atcgattggc ctgttatgaa
    17581 aataaagcta tatatgatgt gtagctatac cttttgtttc ttttaaagcc agtgatttct
    17641 tggatgattc caatatttac aagacgattt atgagttcgt tggcagccgg atagcttgtt
    17701 tttattattt cttttatatc atttatagtg ataataggtc gaatgtataa atagtccagt
    17761 actttaaagg catttgccgc cattcttcca agatgttgag taatcatagc ccgatgattt
    17821 tcacgaagat tcagaatatt tcttgctgtt gttgcagctt cattgcttac gacagttatt
    17881 ccccttaaaa aaaaggataa ccatgattcc caatcaccag tatccctgat attttggaga
    17941 agctcatagt atttttgctt gtgttgtttg aaatagtatg aaatgtataa aactggtttt
    18001 ttgagcattt cttgttcagt tagtataaaa gtaatcagta aacgacctaa tcttccattt
    18061 ccatcaagaa aaggatgaat ggtttcaaat tgtgcatgtg cgagtccaat cttaatcaat
    18121 aaaggtatat ctatctgagc gtgaaggaat ttttcccaat cgcttagtgc ttgaggcaca
    18181 taaaatggtg ggggaggaac gaaaactgct tctcttagtg tacaaccctc tggaccaatc
    18241 cagttctggc tttgacgtaa ttctccaggt gatgcatgtc gtcctcgcac attctgaagg
    18301 agtatagaat gaatttctcg gattaaacgc actgaaagag ggagttcatt taaccgttca
    18361 agaccataat tcatggcctt gacatagttg attacttcag ctacatcttt tggtgcagtt
    18421 tcagaaagaa tttcagcttc ggcttctaat aaattttgca atgagctttg agtgccttca
    18481 atttgactcg aaagtacggc ctctttacga acatacatag caacgaataa atcaggattt
    18541 ggaatgattg tgatggatcc atcaagcctt cccaaagctt gatcggcttc tgatagtata
    18601 gtctgcagtt cgccctctaa ttgtatgggt ggattaggtg gaagaggaga tggtataaat
    18661 gcgttatatc cagaaggttg ctgaatatat acgcccgctc gattgctctg tgtcatcatt
    18721 aaacttaact ttaatatgga gtgaaaattt ttccatgtta aaggtacact ttaatatgga
    18781 aagcgttttg atttatggta aaggaatctt taatataatt tatcaggatg atattgtagc
    18841 cccgccgaag gcggagctac aattaataca aataatttat tctacgatct tcaattcatc
    18901 tatcaagcga tgacaacctc ctaatttatc gataataaac aatacatacc gaatatcaac
    18961 cgaaatagtg cgctgtaaat taggctcgaa ggcaatatct ccactcatgg cttcccaatt
    19021 accatcgaaa gccaagccaa tgagctcgcc gtttgcattc attaccgggc ttcctgaatt
    19081 gccccccgtt atatcggtat tggaaagaaa acaggttaca atatcggtac gcccatgaat
    19141 ctgagtgcca tatctcccaa aatcctttcc ctcgtataaa tcctttaatt ttttgtcgac
    19201 tataaattct ggattatcgg ggtcttcttt ttccattact ccagttaaag ttgtttgcaa
    19261 atcgtaatga acagcatcgg caggataata atcaagaata ctgccatagg tcattcgcaa
    19321 agtactattg gcatcgggtg caaaaagttt cccgggattc atttccctaa gacctgcaat
    19381 ataaagtctg cttcctttat gttcttcctt ttgtgcattt tccacttggc tgtaaagcaa
    19441 aataaaatga ttcctcacct ctgtgccaat ttccagtgca ggatcttttt tcagcacttt
    19501 taaagaagga ttttgcagaa atgccataaa ccgcttctca tcggcaaaaa tgcttttttt
    19561 ataaatgtcg tcagaccatt tatcgaagtc acccttgtat tttttctcta agtctttaaa
    19621 aatatccgcc cgcatctcgg gtgaaatatc acgataatac agttgcaaca tttgagcaaa
    19681 aagtttttta tcggttgaag gattgtagtc tttataaaaa tcttcagccc ttttagaaag
    19741 tttttcgaga gtgttttgaa attttttgtt gagttcttct ttttcagttc ccttggcttt
    19801 ctctattttt tccattaact gaggcagatt ggaacttcgc atagcccaaa ggataatttc
    19861 aggtccttgt aaaacagctt ctcccacgta agtgataggt aggacatagg gctgttgatt
    19921 atgaagagat gtttggatta gttcgagggc ttctccatag cgggctttac gttgtgggtc
    19981 ttgagctacc caattgagaa attcttcttc ggtttttaat ttcttttcgt aaacattcaa
    20041 gcgttttaag cctcgaattt gccctatata atatttccag taattacttg ttgcagcata
    20101 tttggccgca tacatgagat taattctggg atcggtctgc atatcttctt tcatgatttt
    20161 taattttagg tcacgaattt ttacgatagc aggttcggtt tggtcaatag cctgttttac
    20221 ctcccaagaa gtcatataac gatcggtacc accagggaat ccccagatca ttgcaaaatc
    20281 acctttttgt acgcctttga tagaaaccgg cagaaagtgc ttgggtttta gaggaacatt
    20341 ttctttgctg taaggagccg ggctaccatc tggagcggta tagattctga aaatactaaa
    20401 atcgcctgta tgacggggcc acatccaatt atcggtgtct ccaccaaatt ttcccaccga
    20461 cgaaggtggt gcacctacca gtcttacgtc ttcataagtt tgatacacaa ataaataata
    20521 ctcattccca ttgaaaaacg atttgaccgt acaattatat tttttccctt cggatgcttc
    20581 cttgacgatg gagtctgtaa cttctgatat ggcctgttct ctggcatctt catccatttc
    20641 gggagtcaca ttttgtaata cggctttagt aacattttcc atgcgaacaa gcaatgaagc
    20701 cgtgagtcct ggattgggca gttcttcttc gaaactcatc gcccagtaac cattcgtcag
    20761 aatgtcgttt tcaagtgtgc tgtgttcttg aatgtaattg tatccgcaat gatgattggt
    20821 aaatagcaaa ccttgatcgg atacgatttc tcctgtacag aaatatcctt cgggttgtga
    20881 accggcagct aaaccaacta ctgcgtcctt caaactcgag tgattgatac tgtatagttc
    20941 ttcgggtgtc agccttaatc ccagtttttg catatcggta tagttgagac gctcgataaa
    21001 catgggaagc cacatccctt catcgggagg tgtgattatg gctgcttttc ctgacaatac
    21061 actggctatc agtattaccg acacaaccag tttaagtttt aaatttttca tatagtttgt
    21121 tttaagtaat tgaatgagat aacagaaatt taccatttac aaaattaaag agaggccaaa
    21181 attaccaatt ttaagaaata tttgatttct ttgcactgcc aaaatctctg acgatgcgat
    21241 atcagaatga tacacgaata gttatgaccc tcgatggggg agggacaaat tttgtattca
    21301 gtgccatgca ggccgaaaaa gaaattatca cgccatttgc ttttccttca caggcttcta
    21361 ctttatcaga gactttatat aatattgaaa cgggttttag aagaataaaa agcttacttc
    21421 ccggcgagcc cgcagccatt agttttagtt ttcccggtcc cgccgattat gaattgggca
    21481 ttattggcga tctggtcaat ttatcggtct ttcgtggagg tgtcccctta gggccttatc
    21541 tcgaagatat atttaagatt cccgttttta tcaacaatga tggggatttg ttcgcctatg
    21601 gcgaagccat atcaggattt ttgcctgaag taaacagatg gcttgccgat gctggcagcc
    21661 ctaaacgctt tcagaattta ttgggagtta ctttcggaac gggtttcggt gcaggaattg
    21721 ttttaagagg agaattgttt ttgggcgata actcggctgc tggtgagatt aaccgaatga
    21781 gcaataaact ttatcgcaac atgagtgtag aggaaagtgt gagcatccgt ggggttagac
    21841 gcgtattttc caattattcc aatattccat tcgaccaaac acctagcccc aaggaaattt
    21901 atgaaatcgc cattggcaag aggccgggca atcagaaggc ggcaaacaaa gctttcgaag
    21961 aattggccat agtagccggt aacgccattg ccgatgccct taccttggtc gatggccttg
    22021 tggtgattgg tggaggcttg tcgggcgcct ggccagtatt tttaaactgc ttggtggaag
    22081 agcttactct cccctttact acttttagcg gacaaaacat cgaccggctc gaaataaaat
    22141 tttttaatct ggaagatgga gcacagagag aacttttcct gaaaggttca acccgtaaaa
    22201 tcgaaattcc tggcagtgcc agaaaaatta cctacgaccc ggaacatcgc tcaggagtcg
    22261 gaattacacg cctgggaact tcgagagcaa cagcactcgg cgcatataac tttgctctgc
    22321 gcaaactcga tagtatgaaa taatttctaa gcctgatttt taattttgat tttcccctga
    22381 aatactctaa tatatattga atttcctaat gtattcatat acagtcaaag gctggaaata
    22441 tcttaaatct ttgccttggg cataagcttg ccgaatcatg ctcgacgaaa tatctatcaa
    22501 aggcgaagca ataatcttta ccgaagtatg attagcaaat tttcctcctt cgaatcctaa
    22561 acggcaataa acataaatct catgcttctc taaaatgagc tctggatcct tccaccggtc
    22621 gaaatattgt aaattatctc ctcctattac caggacgaat ctttcttgtg gatagatttg
    22681 tcttagatac tctaaggtat gaacagtata agagggtgtt ggaagatgaa attctatatc
    22741 acttgcgcta aatcgcctat catcctgaat ggccaattct accatcctta gccgatgccg
    22801 atcttctgcc agcaattcct tctccttaag tggatttcga ggagaaacta caaaaattat
    22861 ctgctttaaa tccgtatatt ctacaagata attggcaatc attaaatgcc cgttgtgaat
    22921 agggttgaac gagccgaaaa ataaacccgt cttgttgtta ttatttttca taatgtcttt
    22981 tctgaatttc tctatgtcga aagcaagtaa tgatatgatc atttaccacc ccagaggcct
    23041 gaagaaagga ataaataata gttgaaccaa caaaaataaa tccttctttt cgcaaatctg
    23101 cactgatctg atcagaaaga gaagtatgac caggaatttc attataagtt ctataattgt
    23161 tcactatggg cttcctatct acatacgacc ataaatattt atcgaaactt cctctttttt
    23221 ctactaaatc caacatacat ttcgcattat gaatagtggc ttcgactttc gaacggtttc
    23281 ttactattcc cgaattcaat agtaattcat ccgttttttt cttattatag ctcgctattt
    23341 tcgaaaaatc aaaattatga aaagccttac gaaaattttc tcgtttatga aggatagtcc
    23401 tccacgataa acccgcttga aaaatatcaa gcactaaaaa ttcgaacaat cttatatcat
    23461 catgcaacgg aacaccccat tcttcatcat ggtatttcat catgcgttca tcactcaacg
    23521 accagggaca acgttctgct tcattcattc ctgcatggtt tctttatctt taggtttcaa
    23581 aattataaat agagtctgtc catacagtca tcaatatttt cagtaaaagt ataaacttct
    23641 tataaaagta tacctttttg aagttattct tggtcagttt tataattttt cagtctaaaa
    23701 ttaagaactt cttttcaaac tcctctaaaa attaagtatt ttttcactac tgaccggata
    23761 aaatttcaat aaaatggggc tactcccgcg aggtttcgcc ccgatgctgt aagtttgttt
    23821 tgctaaaaaa actgcaacat gggtaaatat acaacaaaga atttagtcgg tcagccggtt
    23881 tttaaacagg ttatgaaaat tcttccgaga gagccatttg atcttgtggt gaggcgttgc
    23941 ggaagcgacc gctactacaa gtcctttttc tcatgggatc agctggtggt gatgctgttc
    24001 gggatctttt cccgctgcga ttcgatgggc gaagtatgtg atgggatgcg ggctttggca
    24061 ggaaagttca attaccttgg gatggacagc gcaccgtcca aaagcacggc gggtgatgcc
    24121 ttgcgtgacc gggacaatga ggtttttcag gttgtactat tttgagctga tcgggtattt
    24181 tgagccgttt ttgccggtca gccgaaaaga gaagatcagc tttgagaagt tttatgcctt
    24241 tgattcgacc acgataacgc tgttttcgga cgtcatgaaa gaggttggcc gtaatcccaa
    24301 gggtgatggc aaaaaaaggg agcttgaaag tacacatgct gaccgatgta cacgcggaca
    24361 cggccgtatt tgctacaatc agcgaggcaa aaatgcacga taagaagttc ttacagcatg
    24421 taaacccgtc aagaggcagc atgttggtct tcgacaaagc ctacaactac tacaggcagt
    24481 ttgccgagtg gacagatgaa gaggtctatt ttgtgtgccg gctgaaagat aacgcgaagg
    24541 ccgaagtcca ggaagttctt catgaaacaa cgcttggcaa agaggagcat ggggtgtacc
    24601 gggttgaaca tattcacctg aagtatacaa aggaaaaacg ggaaaggaca ctttgcctta
    24661 ggttggttta ctatcgggac gaacagggac gaagttacaa attcatcacc aacagctgga
    24721 atataacggc ggaagaagtc gccttgattt ataagtaccg atggacaata gaaacggtct
    24781 tcaaaaaaat gaagcaaaat ttccagctgc attttttcta ttccgacacg gagaacggca
    24841 taaaaacgca ggtttggtgt acgctcatcg cccacttgtt gctcaacgta atccggttac
    24901 tatccaggag caagaaagca ttctcaacgg tagcggcatt catacggata cacctgatca
    24961 gccatttaga catgatgtgg atagtagccg agggcaggcg cacctacacc aagcacacgg
    25021 caagccgcaa caaaagccct gcaactgtac aaatgtccct cttttgaaag ggggtaggat
    25081 ttttaaaacc aagaaaccga tttgtaagta attgactgtg aagaaaataa aaacaaaaag
    25141 gataaaaatt gaatttttgt cggttgatag tgaaaaatta tatgtaatac tctactgata
    25201 tcaattaggt gtttatggtt catgttatca ttatccaaga tcaagacata tcattttgcg
    25261 cttgaaagtt cccttcatat atattagtgc attttggcgc caaaaggtaa cccccctttg
    25321 acactttttt tgaaaattta acatatgaat aatctctgca taaaatatgt cgaacgatta
    25381 aataaatggt tatcagtatg ttgttacatg gtattttaat aaaggaacga tgcagcatca
    25441 atagttggta ttaatcttat aaatgtcgcc ttacaatgct gtgaattctt gctttgttat
    25501 aagattcgga gtatgtattc tatttcgggc ttggaatatc atcataatat cacctgcact
    25561 tcatcacctt ttgcataagc acccttccgt tttgcagagt agccctcacc atataaagcc
    25621 cggcgggcca ggtgcgggtg tcgatacgta gaggattttc tgcgttaagg gcgggcaggg
    25681 acatcttttg cccgttcatg gcatagattt caatccctgc aaggtggccg tggcttttaa
    25741 cccatacctc ctccccggca gggttgggga atacctcaag ctgggtttcc tgtggagggg
    25801 ctgaaatttc ttcctggcct acccagcaca cccccggtgc ctcacagccc aagctgtcca
    25861 ctaccagaag ccaggcattg gctccttgac ctacgctggc aagaaattgg ttttcggttt
    25921 ctactattgc atacaatgt
//
LOCUS       DAOORU010000013        24949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_2401_length_24949_cov_5.405078, whole genome shotgun sequence.
ACCESSION   DAOORU010000013 DAOORU010000000
VERSION     DAOORU010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24949
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2401_length_24949_cov_5.405078"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1358
                     /locus_tag="PLF20_01935"
     CDS             264..1358
                     /locus_tag="PLF20_01935"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOX79806.1"
                     /translation="MNRVLVATGGGDCPGLNAVIRAIVKRAAREKNWEVIGSIEAFNG
                     ILREPTEIMVLNEKAVSGIHYQGGTILGTTNKGGPFAWPVRLPDGTWEEIDRSDEMLR
                     KLQYLGVDAVINIGGEGSQTISQALYEKGLNIIGVPKTIDNDLLGTDSTFGFQTAVQI
                     CTEAVDKLVTTAASHNRILILEVMGRYAGWIAMNAAVAGGAEICLIPEIPYDIDKVLQ
                     KIDSRFVKGRGFVNIVISEGARPIGGDFARKETGEVGYHSVKLSGAAERLMQELKEAG
                     CPYDMRATVLGHVQRGGVPIAYDRVLATQFGVKAMELVMEGKFGQMVSYRHPYITSVP
                     LKDAIKRYNFVDMDTALIKTARGVGICFGD"
     gene            complement(1457..2692)
                     /gene="clpX"
                     /locus_tag="PLF20_01940"
     CDS             complement(1457..2692)
                     /gene="clpX"
                     /locus_tag="PLF20_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOX79807.1"
                     /translation="MAKTLEYCSFCGRSKKEVNLLISGINANICDSCIEQARDIVLQE
                     ITSARKKKVHSKKIYKPAEIKAYLDQYIIGQEEAKKVLSVAVYNHYKRISQPISQNNI
                     DDVEIEKSNIIFVGETGTGKTLLAQTIARLLNVPFCIADATVLTEAGYVGEDVESILA
                     RLLQAADYDIATTEMGIVFIDEIDKISRKSDNPSITRDVSGEGVQQALLKLLEGSIVN
                     VPPQGGRKHPEQKFIQVNTQNILFIAGGAFDGIENKIASRLRTNIVGFKKEPFTEIEK
                     DNLLQYVSPPDLRAYGLIPELVGRFPVVCHLSPLDANALKRILTEPKNALVKQYKKIF
                     EMDKIQLSFDDEVFDFVVQKSIEFKLGARGLRSIMESILKDLMFEAPSGKLAPTLHIT
                     LDYAQEKFETSGISRLKVA"
     gene            complement(2742..3449)
                     /gene="clpP"
                     /locus_tag="PLF20_01945"
     CDS             complement(2742..3449)
                     /gene="clpP"
                     /locus_tag="PLF20_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOX79808.1"
                     /translation="MNNMNKDEFYKFATRHVGINPLALQKYIAVHDNYISPTIIEERQ
                     LNVATMDVFSRLMMDRIIFLGVPIDDYVANIIQAQLLFLESTDPRKDIQIYLNTPGGS
                     VYAGLGIYDTMQYIAADVATICTGMAASMGAVILCAGAKGKRTALKHSRILIHQPMGG
                     AEGQASDIEITAREIQKLKKELYEIIAYHTGQPFEKIWQDADRDFWMTSYEAKEYGMI
                     DEVLFRERNTKDSSNQA"
     gene            complement(3442..4872)
                     /locus_tag="PLF20_01950"
     CDS             complement(3442..4872)
                     /locus_tag="PLF20_01950"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOX79809.1"
                     /translation="MEIIREDTGELTATLHLKLSEEDFLPQIVAELNKLQREAVIPGF
                     RKGKVPMEHIKKLYGYSLFFDYINKLLIETVIQYITENQLPILGYPLINQQAGSFNME
                     YYQGEEPEFLFDIAFTPEIEINFSEIQPVKYIKIVPTQTDIDRQIEKFREQFAELVEI
                     ETVDGEAIIEGELFEIDNSGSPKEDGYHQPIRFDIREESLPQEIKDKLLGLRKDEIID
                     FLFGSSEDLAKFIGISLSEAENLYKNLQLKIISISHKKLPELNQDFFHQVFPEQNIET
                     LEEFRAEVAKKINLNVLQEYSDRLFYLASMQMILNQTNIPLPKDFLKRWIATDENKDL
                     EEVDKGIDDYIESIRIDLLINYIIEKADIKIDEEDIRNHLAQKIVSSSFPYLQNNPNI
                     VEKISQYVDAAMKNEKNIENAENELRERKIIEFLKSNLPLEITELDSTTFMASITKDT
                     KKEDKEAQEEKSNEIMENNTENQNNE"
     gene            complement(4981..5274)
                     /locus_tag="PLF20_01955"
     CDS             complement(4981..5274)
                     /locus_tag="PLF20_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79810.1"
                     /translation="MASKKDTKKYIEYAIDEVISDCLNVLHLYPGKKEEEVYHLLREL
                     VNTRNNLVYRVNHIEGKDDPRAVKAHFRSIENDLNHQIEQAIEKLSNIVKSND"
     gene            complement(5329..5410)
                     /locus_tag="PLF20_01960"
     tRNA            complement(5329..5410)
                     /locus_tag="PLF20_01960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5374..5376),aa:Leu,seq:tag)
     gene            5545..5766
                     /gene="yidD"
                     /locus_tag="PLF20_01965"
                     /pseudo
     CDS             5545..5766
                     /gene="yidD"
                     /locus_tag="PLF20_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
     gene            complement(6055..7065)
                     /gene="rlmN"
                     /locus_tag="PLF20_01970"
     CDS             complement(6055..7065)
                     /gene="rlmN"
                     /locus_tag="PLF20_01970"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOX79811.1"
                     /translation="MDNSANLIGKNLEELRNIFMAAGYETKDPSLLAHNLYKKYGKPL
                     SSIEAYPKSLRWFLEDNFSIEVLQPILKLQSSDGTIKGLFESEGGKAFEAAWIPAAKR
                     MTICVSSQAGCRFGCKFCSTGQHGFRGNLSTFEILSQVFGLRKPFNHIVFMGMGEPLD
                     SYQSVKKAVEILNAWWGVSLANRYITISTVGIFPELSKVIEELKCNLAISLHSPFHEE
                     RKSIMPVESIYPIKEVIKMLKTKKFTGKRRLSFEYLLLGGFNDSPLHAHETGRLLSGL
                     RCHINLIAYNSIAGANFRRPCLRTIENFREILNEYGLRVTIRISSGYDISAACGMMVG
                     SK"
     gene            7437..8516
                     /locus_tag="PLF20_01975"
     CDS             7437..8516
                     /locus_tag="PLF20_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOX79812.1"
                     /translation="MKSLKFTVCLFFALLAGQLYAQDASTEQKLEHLMKKVQDLEHEF
                     DILKKLSDDILWFDRVGDMAHIDKVYITGPPPQVVKNPDATGARNPLKFYCYVFIPRQ
                     LDYSKKYPLIILPHGGVHADFTTYHTHIIREMIYQGYIVIAPEYRGSTGYGKSFYEKI
                     DYGGLEIEDVHAARNYMVENYPFVDKNRIGLVGWSHGGLIALMDVFEHPSDYAVIFAG
                     VPVSDLIARMGYQGPSYQELFSADYHIGQTAAENTAEYRRRSPAWNAEKLQTPLLIHT
                     NTSDDDVNVLEVEHLIKSLKAANKKFEYEIFQEVEGGHSFDRIDTKTAKEIRVKIYRF
                     IGTYLKPPRQIQTVDQINKAAYFFE"
     gene            8575..8647
                     /locus_tag="PLF20_01980"
     tRNA            8575..8647
                     /locus_tag="PLF20_01980"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8608..8610,aa:His,seq:gtg)
     gene            complement(8885..9418)
                     /locus_tag="PLF20_01985"
     CDS             complement(8885..9418)
                     /locus_tag="PLF20_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOX79813.1"
                     /translation="MENLEKIKERIEVAQGDITRCEVDAIVNAANTSLRGGGGVDGAI
                     HRAAGPLLLEACKKLDGCPTGDAVITPGFNLKARWVIHTVGPVWRGGKHDEAHLLASC
                     YRKCLSLSFENKFGQVAFPNISTGIYGFPKKDAAQIAIHTVIQFLPSHEFPQKVIFVC
                     YDNENYSIYQEILSSLF"
     gene            complement(9430..10806)
                     /locus_tag="PLF20_01990"
     CDS             complement(9430..10806)
                     /locus_tag="PLF20_01990"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOX79814.1"
                     /translation="MKHLFLIMTFLGMGLFLLAEQTYTVKKKTLPEKVGKVYVIEMKE
                     EIAPPMRRKVQKGFGEAREKGVDLIIIHMNTYGGTLDAADSIRTIILTSPLPVWVFID
                     NNAASAGALISIACDSIYMRPGANIGAATVVDQSGGALPDKYQSYMRSMMRSTAEAKG
                     RDPQIAQAMVDTRIVVPGIDDSTTVITFTTSEAIQYGFCEGETENIQQILKKYGWDDA
                     VIITQKLSGVDKIINFLIHPIVSALLIMLIIGGIYFELQTPGIGFPLAIAILGAILYF
                     APLYLEGLAAHWEILLFIIGLILIAIEIFAIPGFGVTGISGIILVVAGLTLSLVGNFN
                     AIPAGQIVQDILKAFFTVIIAIFISLISSFYGSKRLFSHPVPFLGSLALDTTQDKELG
                     YTIANEDYSSMVGKTGFALTILRPAGKIMIEEEVFDAVAINGYISKGQEVEVVRYENA
                     QLYVKVKN"
     gene            complement(10860..11651)
                     /locus_tag="PLF20_01995"
     CDS             complement(10860..11651)
                     /locus_tag="PLF20_01995"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOX79815.1"
                     /translation="MNFKEIAFEVESIAKEAARYILEQRKSLSESDVETKGLHNYVTY
                     IDKGSESLIIDHLKPLINNAGFIAEENTQNLRGKEFNWIIDPLDGTTNFIHGVPICSV
                     SIALMQNSEIVVGEVLEIFSGESFLAWKDGPALCNGKPVQVSRRSNVKEILLATGFPY
                     YDYDLLDQYLALFKHFMKNTRGLRRLGSAALDLAWVACGRFDGFYEYGLNEWDVAAGS
                     FIVERAGGKCSDFEGKPHHVFGRQILSSNGILHQQLLNQIQMFFP"
     gene            complement(11648..12817)
                     /locus_tag="PLF20_02000"
     CDS             complement(11648..12817)
                     /locus_tag="PLF20_02000"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOX79816.1"
                     /translation="MYPKISDLINDIFGTHINLPIQSYGMMMAIAFVVGGLILNSELK
                     RKEREGILKPHTKKIIEGLPATPIEIALSGFITFILGFKIIGIILDYNFFADHPQEFI
                     LSWKGSWLGGLLAGGFSAYYIWHSKEKKKLPKPKTKFVEIHPHQLTVNILFVAVVFGI
                     IGAKLFDVMEHLDELVRDPLGTLFSFSGLTFYGGLILAAIAVVFYARKYGIAFEHIAD
                     ATAPALILAYAVGRIGCQLSGDGCWGIPNTSPKPHWLSFLPNWLWAFNYPHNVINEGI
                     PIPGCEGQHCFVLQQPVFPTPLYETIICTLFFLVLWGIRKKLRIPGLLFGIYLILNAF
                     ERFFIEEIRVNRNYHIIGPIELSQAQIIAIAIFITGVVFSIVFYRKYKHLQTQRI"
     gene            complement(12844..14151)
                     /gene="mtaB"
                     /locus_tag="PLF20_02005"
     CDS             complement(12844..14151)
                     /gene="mtaB"
                     /locus_tag="PLF20_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOX79817.1"
                     /translation="MNSIIVAFHTLGCKLNFAETSTLIRKFRELGYQITDFDKEADIY
                     IIHSCSVTSVAEKKSRAAIYQAHRRNPKAFIAVIGCYSELEAKKLKAISGVQWVLGNQ
                     EKFRITELISRDFSTNLEVSVTSLDSEPSKNLQEFHPAYSSGDRTRSFLKIQDGCDHF
                     CSYCSIPYARGKSRSPSVQEALQWAQKAAADGVKEIVLTGVNIGDFGRKNGESLYDLL
                     QLFDKGRIVKRVRLSSIEPELLSDDIIRLIAESRVLMPHFHIPLQSGSDTILKRMKRK
                     YTTKVFESRVKYIKQLMPYACIACDIIVGFHGETDELFEETRQFIQSLPLSYMHVFTY
                     SERSNTPAASLDGKVSPQIKHQRSQMLHQYSAAKLDQFYKLCQHQWVNVLFESENTDG
                     MMSGYSENYIRVETPFREDFVNQIIHLPLEQRNVTGNYAYYPR"
     gene            complement(14196..15101)
                     /locus_tag="PLF20_02010"
     CDS             complement(14196..15101)
                     /locus_tag="PLF20_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX79818.1"
                     /translation="MKSQNKAYLFAGLAILAWSTIATAFKITLRYVSFVELLLYASLT
                     STLFFTIYLIINKRLRNVFDRPHKELLRLAFNGILNPFLYYLILLKAYELLPAQEAGT
                     LNYFWPVVLVLLSIPMLGQKISWMSIISVFISFIGIVIISTHGKPWLMQFSNPLGVVL
                     ALTCPFIWAIYWILNMKIAGDEVEKLFIGFLSGTILIIIYSAIKHSLTFPPIKGLAGA
                     IYVGIFEMGLTFIFWLKALKYSKTTAHISNLVFLSPFLSLFYINFILKERIMLSTITG
                     LILVVVGIIMQQYVPDIEKKGRKAQ"
     gene            complement(15112..16095)
                     /locus_tag="PLF20_02015"
     CDS             complement(15112..16095)
                     /locus_tag="PLF20_02015"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOX79819.1"
                     /translation="MFCINNQNTNPYFNLASEEYLLKNYEEDVFMLWRNEPSIIIGKH
                     QNALAEINIEYVKENHIKVVRRITGGGAVFHDLGNLNFTFISNSPNSKPVMNFKKYTQ
                     PIVDVLVNLGVDARFEGRNDIVIDGKKISGNAECVYKNRFLHHGTILYSAHLPNLSSA
                     LKVDPVKFQDKAVKSTCSRVANVSEYLKKTIDIIQFRDLIFTHISQMYPESKIYEFKP
                     EDITYITNLQNSKYETWEWNFGNSPEYNFRKIVKTSGGFVEFDLMVEKGIITSVKIFG
                     DFFSSDEISEIESALKGLPHEYSDVKQKLKEYEIERYFNRASLDEVLEGIF"
     gene            16289..17482
                     /locus_tag="PLF20_02020"
     CDS             16289..17482
                     /locus_tag="PLF20_02020"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX79820.1"
                     /translation="MSKSLSEILFEEGEERERYYNAVSPPLIQTSNFVFSTVEQMRRA
                     LSDEYTHSVYSRGNNPTLAILRNKLAKLEGAEDALVFSSGCGAIAAALFSQLRHGDHL
                     VCIRKPYNWVAHFCRIILPKYGIQTTFVEEGSARAYEAACQPNTKLFYLESPNTFTFE
                     ITDIRAVAEIAKSKGIVTVIDNSYSTPLYQSPLSLGIDLVLHSASKYLAGHSDVIAGV
                     LAGSKEMIGKIYNSEYLAFGAVLPPFEAWLMLRGLRTLEIRLQRSSSNAKALIEAIEN
                     HPRIKRILYPFHSQHPKNEIARSQMKDCSGLFSIELEADTPDEVILFCNSLKHFLKAV
                     SWGGFESLVDPAITVYNFDHPELNELSWRTVRFYCGLESPEILIDDVLQALNILDNGA
                     TNRQK"
     gene            17484..17852
                     /locus_tag="PLF20_02025"
     CDS             17484..17852
                     /locus_tag="PLF20_02025"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HOX79821.1"
                     /translation="MARLLKSFEYAGRGLLEMIRSQMNARIELFILTVVMIAGFVFKI
                     RVNEWITIILVSGLVLAAEAFNSAIENLANAISLRQFDSKIKKIKDLAASGVLISAVC
                     AFVIGLIIFIPYIVRFFVSL"
     gene            17849..18697
                     /locus_tag="PLF20_02030"
     CDS             17849..18697
                     /locus_tag="PLF20_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HOX79822.1"
                     /translation="MILIVDSGATKAEWRLLLENGDVQAANTTGINPYFIRSESIGNI
                     LMNELPDNWIHESKIGKIYYYGAGCGSEIQEKSIEKALKAIFPEANLFISSDLLGAAR
                     ALWQRDSGYTAILGTGSNAGYFNGQQIEKGIKSLGFILGDEGSGADLGKRLLKRYLEG
                     KFNQDLAENFYQQFPFDLADFLDAIYSKPFPNRFMAGFVPFMKENSNEIEIQLIVEEA
                     FDDFFGLVQTIAQSSFRKSIRFTGSVAWAFEEILLGVAAQHHFEVSLVVKNPIDLLVK
                     FHHAQP"
     gene            complement(18701..19198)
                     /locus_tag="PLF20_02035"
                     /pseudo
     CDS             complement(18701..19198)
                     /locus_tag="PLF20_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19640..19714)
                     /locus_tag="PLF20_02040"
     tRNA            complement(19640..19714)
                     /locus_tag="PLF20_02040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19677..19679),aa:Val,seq:tac)
     gene            19909..22272
                     /locus_tag="PLF20_02045"
     CDS             19909..22272
                     /locus_tag="PLF20_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOX79823.1"
                     /translation="MYDKYFIGYILLIVILSGCNPTKKLPERKFLLTENKIEIEPEKQ
                     MKASEIQKYILQTPNKKFLGMFHINLWLYNETANWKASKFRDWLRRLGGKPPAIYDPN
                     LTLSSERNIKNYLYNKGYFNATIKSQEILQDKKAEVVYHIKLNKSFKIRNFLLEAEDT
                     LVSRYANRIMANTPIRPGEIYDYYVLEGERERLTVEMRNAGFYFFTNDYIFYEIDTTI
                     GQKNLDIRMLILNPSNFTGFDPGRANEWLALHHLYKIRNIYVFPQYSSHSETNSFSDT
                     LVYNRTGTDDTSQAKNDILYLIYNEPLKIQPKIISQAIYIHSGDLYCLRNADLSFRAL
                     AEFPIYRSINIEFRLADTAGISLTRHGHLDCYIYLSRAEVQGFTIETEVTNTGGDLGL
                     SGNLVYTNRNVFRGAEMLRLKLRGAAEMQKSFEQTSQNKLLLFNTYESGAELNLTFPR
                     FVWPLKGLRFPSSMRTKSNLLVGYNYQNRPDYIRHITNAAFSYQLKVSDFKSHLLTPL
                     ELNAIKIYATPSFQQLIDSIDDQGIRSQYSDHLIPALRYSYIFTNQEIGKFHNYKYFR
                     FDFESSGNTLYAVDRLTNVSPNIHNEYTFLGIAYAQYLKASSDFRYFYMFNLNNTLVM
                     RIFAGAAIPYGNSNYLPFEKGFFAGGANGMRGWTLRMLGPGHFSSEGGQRYNRMGDLH
                     FETNLEYRFPMYNFFKGAFFLDAGNIWLVKSNPMYPGGLLNARTFLKEIAMDAGIGIR
                     LDFTYFVFRLDAALPLLDPARPEGSRWLLPKSRLSDLVWNFGIGYPF"
     gene            22307..23596
                     /locus_tag="PLF20_02050"
     CDS             22307..23596
                     /locus_tag="PLF20_02050"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOX79824.1"
                     /translation="MTYTEAVDYIFQHLPMFTKIGGVAYKADLSRTLYLCEKLGNPHL
                     NFRSIHIAGTNGKGSVASFLSSIFMESGSKTALFTSPHLRDFRERITINGILIPGDYV
                     AEFIEENRPNFETIQPSFFEMSFALAVKWFSDQKVDVAIIETGMGGRLDSTNVILPEI
                     SIITNIGLDHQQFLGSTLAEIAAEKAGIIKPNIPVVIGEHNQQTDRVFIEKAIQTHSL
                     LFWAEDLSSVEDSFYDVENNYLNLSLMILSGKLIQLQSPLPAEYEIKNIRTVMAAAEV
                     LNQSGYSLSDEILANGIKNVLKNTGLKGRWQILSKNPLIVADISHNEPGIKELMKQLN
                     KLKYNHLHFVLGMVSDKDSFSILQLFPKEATYYFCQPNIPRGKDVEQLADEAHEFGLQ
                     GKIFHSVEEAFRTACASALADDMVLISGSAFVVAEII"
     gene            23803..>24949
                     /locus_tag="PLF20_02055"
     CDS             23803..>24949
                     /locus_tag="PLF20_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HOX79825.1"
                     /translation="MILNYIWFGFFIIAFVIALVHLIFFQDSSVFDAILQSTFDNAKV
                     GFELSLGLTGVMTLWLGVMRIGEKGGMVEILAKLINPLFRRLFPEIPANHPVNGSILL
                     NIAANMLGLDNAATPLGLKAMNQLQELNPEKEKASNAMIMFLVINTSGLTIIPVSIMV
                     YRAQLGAANPADVFLPILLSTFVSTLAGIISVSLVQRINLVQKTVLLYLLGFTAIVGG
                     LVWYLSIVDQQTMNKISTAISSVILFSIIIIFMVIATVKKVRVYDEFIEGAKEGFHIA
                     VRIIPYLVGMLVAIGVFRASGAMDSLVDGISWCAQKAGLDTSFVPALPTAIMKPLSGS
                     GARGMMLEAMKYFGADSFVGRLSCLFQGSTDTTFYIIALYFGSVGIRN"
BASE COUNT         7301 a         4833 c         4996 g         7819 t
ORIGIN      
        1 gtacatagaa gaagaatgcc tgttacaatc gactttttca cggtaaaaat tctcgaaacg
       61 aatttgaatg agagtgcaaa tttaataccg gaataaacac ccggaaacat attccttact
      121 ctatttcctc tataaatctc ataatttttt tataagggtc ttataacgga caattatgaa
      181 ttttttttat ttaagtttgc atatagagta aaaaagttgg aatcaaaaag gaataaattc
      241 ataacaaaac caaattattg aaaatgaaca gagtattagt agctacgggt ggtggcgatt
      301 gccctggatt gaatgcagta attcgggcta tagtaaaaag agcagccaga gagaagaatt
      361 gggaagtgat aggaagcata gaagctttta atggaatttt aagagaaccc accgagatta
      421 tggtactcaa cgaaaaagcc gtttcaggta ttcattatca gggaggtact atattaggaa
      481 ccactaataa aggaggtcca tttgcctggc ctgtaagact acctgatgga acctgggaag
      541 aaattgacag aagcgatgaa atgcttcgca aactgcaata tttgggtgta gatgcggtca
      601 tcaatattgg aggagaagga tcacagacca tctcgcaggc tttgtacgaa aaagggctca
      661 acattattgg agtaccaaaa accatcgaca acgatttgct gggaaccgat tctactttcg
      721 gatttcaaac tgccgtacag atttgtactg aagctgtgga taagcttgtt accactgctg
      781 cgagccataa tcgtattttg attcttgaag tgatggggcg ttatgccgga tggattgcca
      841 tgaatgcagc tgtggctggc ggagctgaaa tatgcctaat tcctgaaatt ccttatgata
      901 tcgataaggt tcttcaaaaa atcgattccc gcttcgtaaa gggacgaggt tttgtgaata
      961 tagtgatttc ggaaggtgct cgtcctatag gaggcgattt tgcaagaaag gaaaccggcg
     1021 aagtgggcta tcacagcgtt aaactttcgg gagcagccga acgcctcatg caagaattga
     1081 aggaagcagg ctgcccttat gatatgcgag ctaccgtttt aggccatgtg cagcgaggag
     1141 gtgtgcctat tgcctacgat cgcgtcctag ctactcagtt tggagtgaaa gccatggaac
     1201 tggtaatgga aggaaaattt ggacaaatgg tgtcgtaccg tcatccttac attacctccg
     1261 ttcccttgaa agatgccatt aaaagataca atttcgtaga tatggatacc gcattaataa
     1321 aaactgcgcg tggtgtgggt atatgttttg gcgattgaat cataggaaaa tattttctcc
     1381 aaatttaaag ccctgagtct taccgatcag ggctttaaat ttctttgtca gaataaacgt
     1441 gaatattcaa tccaattcag gccaccttca atctgctgat gccagaggtt tcgaattttt
     1501 cttgggcata atcaagcgta atgtgcagag taggagcaag ttttccagaa ggcgcctcga
     1561 acattaaatc cttaagaatg ctttccatga tggaacgtaa ccctctggca ccaagtttga
     1621 attctatgga cttttgaacg acgaaatcaa atacctcatc atcgaagctc agttggattt
     1681 tgtccatttc gaatatcttc ttatactgtt ttaccagagc atttttaggt tcggtgagga
     1741 tacgcttcaa agcattggca tctaagggtg atagatggca aaccacagga aaacgaccta
     1801 caagttccgg aataaggcca taagctctca aatcaggagg agaaacatac tgcagcaaat
     1861 tgtctttttc gatttcggta aatggctctt ttttaaagcc tacgatattg gttctaagcc
     1921 tcgaggcaat tttattctcg attccatcga aggcgcctcc ggcaataaag agaatattct
     1981 gagtgttcac ctgaatgaat ttttgctcgg gatgtttgcg acctccctgt ggaggaacat
     2041 tcacaatgct tccttcgagg agtttgagca aagcttgttg aaccccttca cccgatacat
     2101 cacgtgtaat gcttggattg tcgcttttac gagagatttt atcgatttcg tcaataaaaa
     2161 caatacccat ttctgtggta gctatatcgt aatcggcggc ttgcagcaac cgagctaata
     2221 tgctctcgac atcttctccc acataaccgg cttcggttaa aacagtggca tcagctatgc
     2281 aaaaaggtac attgagcaag cgcgctatgg tttgagccag aagtgtcttc cctgtaccgg
     2341 tttcccctac aaagatgata ttcgattttt cgatttctac atcatctata ttgttctggc
     2401 tgataggttg tgaaattctc ttataatgat tgtaaaccgc aaccgagagc actttcttgg
     2461 cctcttcctg cccgataatg tattgatcga ggtaggcttt gatttctgca ggtttataga
     2521 ttttcttcga atgaaccttc ttctttcgtg cggaagttat ttcctgcaaa acaatatcac
     2581 gggcctgttc tatacaacta tcgcaaatat tggcatttat tccactgatg agcagattga
     2641 cttctttttt acttcgcccg caaaatgaac agtattccaa tgtttttgcc atcgatttct
     2701 tccttttgta caaaatatct cgagccaaag aaaaagaatc cttaggcttg atttgaacta
     2761 tctttcgtat tcctttccct gaacaatact tcgtcgatca ttccatattc cttggcttca
     2821 tatgaagtca tccagaaatc acggtcggcg tcttgccaga ttttttcgaa aggttggcct
     2881 gtatgatagg caataatttc atacagttcc ttttttaatt tttgaatttc tctggcagtg
     2941 atttctatat cggaagcttg tccttcggca cctcccatag gttgatgaat caggatgcgt
     3001 gagtgcttga gggctgttct tttgcctttg gcaccagcac ataggattac tgcacccatc
     3061 gaggcagcca tgcctgtaca gatagtggcc acatcggctg caatatattg catggtatca
     3121 taaatcccta atcccgcata taccgaacct ccaggcgtgt tcaaatagat ttgaatgtct
     3181 ttacgtggat cggtactttc gaggaataga agctgtgcct gaataatatt ggccacataa
     3241 tcatcgatgg gtactcctaa aaaaatgatt ctatccatca tcaacctcga gaacacgtcc
     3301 attgtggcta cattgagttg acgttcctca ataattgtag gcgatatgta attatcatgt
     3361 accgcaatgt atttctgcaa agccaaagga tttattccta catggcgagt tgcaaatttg
     3421 taaaattcat ctttattcat attattcatt attttgattt tctgtattgt tttccattat
     3481 ttcattactt ttttcttcct gagcctcttt atcttctttc ttcgtatctt ttgtaattga
     3541 agccatgaat gttgttgaat ccagttctgt aatttcgagt ggaaggttcg attttaggaa
     3601 ttctataatt ttacgttctc tcagttcgtt ctctgcattc tcaatatttt tttcattttt
     3661 cattgctgca tccacgtact gtgatatttt ttctactata ttaggattat tctgaagata
     3721 agggaaactc gaagatacaa tcttctgtgc taaatgattt ctgatgtctt cttcatcaat
     3781 ttttatatct gctttttcaa taatataatt gattagtaag tctatacgaa ttgattcgat
     3841 ataatcatca atacctttat caacttcttc taaatcttta ttttcgtcgg tggctatcca
     3901 tcttttgaga aaatctttgg gcaggggaat attcgtttga ttaagtatca tttgcatgga
     3961 agccaagtag aataatctgt cgctatattc ttgcaaaaca tttaaattaa ttttttttgc
     4021 tacttcggct ctgaattctt ctaacgtttc tatgttttgt tcggggaaaa cttggtgaaa
     4081 aaagtcctga tttagctcag gcaatttttt atgagaaatg ctaatgattt taagttgaag
     4141 attcttgtat aaattctccg cttcgcttag gctaattcca ataaatttgg ctaaatcttc
     4201 ggatgaacca aacagaaagt cgatgatttc atcttttctt aaacctaata gcttatcctt
     4261 aatttcttga ggaagggatt cctctctaat atcaaatctt attggttgat gatatccatc
     4321 ctcctttggc gagcctgaat tgtctatttc gaacagttct ccctcgatga tagcctctcc
     4381 atccacagtt tctatctcca ccaattcggc aaactgttct ctaaatttct cgatttgacg
     4441 atctatatcg gtttgcgtag ggacgatttt aatgtacttc accggttgta tttcggagaa
     4501 attaatttct atttcgggtg tgaatgctat atcgaaaaga aattcgggtt cctcaccttg
     4561 ataatactcc atattaaatg acccagcttg ctggttgatt aacggatatc ctaagatagg
     4621 caattggttt tctgttatat attgaatgac tgtttcaata agtaatttat taatatagtc
     4681 aaaaaagagt gaataaccat acaatttctt tatatgctcc ataggcacct ttcctttgcg
     4741 aaatccagga atgactgcct cacgttgtaa tttattaagt tcggctacaa tttgaggaag
     4801 aaaatcttct tcagataact tcaaatgaag tgttgcggtc aattcacctg tatcttctcg
     4861 aataatttcc atggataatt taagttttta ttttgaaagt ttgctcgtaa atattgatgt
     4921 ttaggtttgc cttttctctg tgtcttccca gaatttaggt aaggcagtta taagcatttt
     4981 tcaatcattc gacttaacaa tgttgcttaa tttttcaatg gcttgttcta tctgatggtt
     5041 taaatcgttt tctatgctcc tgaaatgagc tttaaccgca cgagggtcat ctttgccttc
     5101 gatatgattc acccggtaca caaggttgtt gcgggtattg acaagttctc tcagaaggtg
     5161 ataaacttct tcttcttttt tacccggata aagatgaagt acatttaaac agtcagagat
     5221 tacctcatcg atggcatatt caatatattt tttggtatct tttttactgg ccattgggtt
     5281 ttttaagttt taagtaagga tattaaacta ttagaaaata tgttactgtg cggatgaagg
     5341 gactcgaacc cccacgcctt tcggcaccag atcctaagtc tggctcggct accaattacg
     5401 acacatccgc atagggtttg caaaattaca ataatttata agattggcaa ccgaattaaa
     5461 gagaatggct ttcgttgctt aaatggcttt tgttcgaaga ttttcatcgg aaggtattgc
     5521 ttattttcag ctatttttgc aaacgtgctg aagcaaatac taaaaactcc gttgattatt
     5581 ctgataaggg tgtatcaatt tggtatttca ccatttacac ccgccagttg caggcattgg
     5641 cctagctgca gcacttatgc tatcgaagcc atcaaacggt atggcatatt tcgaggtggg
     5701 atgcttgcca tcaaccgtat tttgcggtgt catccgtggg gaactagtgg catcgaccct
     5761 gtcccacttt tcttattcca tcgcttttat tccaataaaa aactaagttg taacaggttg
     5821 aagcctcatc cggaaaattt gcaataagct gaaaatgtac ccattgaatt gtctcgatga
     5881 ttttctggct tcgaaatatc cttcgtaaga gattggaatt ttaaagaact gagcctccta
     5941 tttcattggt ttagcattgc tcgaatgcga ccaaaatagc acgatgaatc tttgaaaaag
     6001 tataaggagt ttgtcgacaa ttcgtaagtg ttcgaaaagt agagatttta atttttattt
     6061 gcttcctacc atcattccgc aagcagccga aatatcgtaa ccagaagata ttctgatggt
     6121 taccctgagg ccgtactcgt ttaatatttc tctgaagttt tcgatggttc gaagacaagg
     6181 tcttctgaaa tttgccccag caatggaatt gtaggcaatc aaattaatat gacaccggag
     6241 gccgctcaaa agtctgccgg tttcatgagc atgtaagggg ctatcgttga atcctcccag
     6301 cagaagatac tcgaatgaaa gcctgcgttt gcctgtaaat tttttcgttt ttagcatttt
     6361 tatcacctcc ttgatgggat aaatactttc aacaggcata atgctctttc tttcttcgtg
     6421 aaatggagaa tgtaagctaa tggcgagatt gcatttcaat tcttcgatta ccttcgaaag
     6481 ttcaggaaat ataccaactg tgctaatggt aatatatcgg ttggctaatg agactcccca
     6541 ccatgcattc agtatttcaa cggctttctt gacggattgg taactgtcga gaggctcgcc
     6601 cattcccatg aatacgatgt gattgaaagg ttttcgaagt ccaaatacct gcgagagaat
     6661 ttcaaaagta cttaaattgc ctctgaatcc atgctgccct gttgagcaaa atttgcatcc
     6721 aaacctgcaa cccgcttgcg atgatacgca gatagtcatt cgttttgcgg ctggaatcca
     6781 tgctgcttcg aacgctttcc ctccctcact ctcgaataga ccttttatcg taccatcgga
     6841 cgattgaagt tttaaaatag gttgtaaaac ctctattgaa aaattatctt cgagaaacca
     6901 cctcaatgac ttaggataag cctcaatgga actcaaaggt ttgccatatt tcttatacaa
     6961 attgtgcgca agcagcgatg gatctttggt ttcatagccg gctgccataa aaatatttct
     7021 tagttcttcg agatttttac ctatgagatt tgccgaatta tccatgtcat aaagctcagc
     7081 cgcaaagata cctatgtttc aataaaatgg ctggtaagga cataggctta gtttagtcga
     7141 tcgacgcatt cagccctcac ctcccaaaat ttacgacatg tggaagctcc aattactaaa
     7201 tttttttaaa cccaataggc ctctaagacc tgttaggtct gaatttcgaa ggaatattat
     7261 tgaaattttg gaggttttta ccactgagac attcaaggtg atccggaacg gttttcctga
     7321 caatttgcaa aaagtatgcg taaataatcc atagcttatg ttttcgaacg ttttcctttt
     7381 gtcgagtgtg atatttataa atcacttata tttacctcgt ttaaaatttt tgggttatga
     7441 aaagcttaaa attcacagtg tgcttgtttt ttgctttgct cgcagggcaa ttgtatgctc
     7501 aggatgcatc cacagaacag aagcttgagc atcttatgaa gaaggttcag gatctggaac
     7561 atgagttcga tatcttgaaa aaactcagcg acgatatttt atggttcgat cgggtagggg
     7621 atatggcaca tatcgataag gtatatatca cggggccacc accccaggta gtaaagaatc
     7681 ctgatgccac tggtgcgcga aatcctttga aattttattg ttatgttttc attccccgac
     7741 agctcgacta ttcgaagaaa tatccactga ttatattgcc gcatggaggt gttcatgccg
     7801 attttaccac ttatcatacc catattattc gggagatgat ttatcaggga tatatcgtaa
     7861 ttgcacccga atatcgtggt tcaacaggct atggaaaatc attctacgaa aagattgatt
     7921 atggtggcct cgagattgaa gatgttcacg cagcacgaaa ctatatggtc gaaaattatc
     7981 cttttgtcga caagaacagg ataggattgg tgggatggag tcatggaggg cttatagcct
     8041 tgatggatgt tttcgagcat ccatcggatt atgctgtgat ttttgcgggt gtgccggtaa
     8101 gtgatttgat agccagaatg ggatatcaag ggccatccta tcaggaactc ttttcggccg
     8161 attatcatat tggccaaacc gcagccgaga atacagcaga ataccgtcgc aggtcgccgg
     8221 catggaatgc agaaaaactc caaacgccat tacttattca taccaacacc agcgacgacg
     8281 atgtgaatgt tttagaggta gaacacctga taaaatcatt aaaagccgca aacaagaaat
     8341 tcgaatatga aatctttcag gaagtggaag gaggacattc attcgacaga atagatacaa
     8401 aaaccgcaaa agaaatcaga gtaaagatat atcggtttat agggacttat ttaaaacccc
     8461 cccgacaaat ccaaactgtc gatcaaatca acaaagcagc ttatttcttc gaataatttc
     8521 tttcatgaag ttttgaaatc agaaaatttt cttaattttg catccaatta tacggtgggc
     8581 gtagttcagc ggttagagcg tcggattgtg gttccggatg tcgtgggttc gaatcccacc
     8641 gtccacccaa aaaatggccg aaaggccttt ttttattgca tcatgcttag ccttcccgtt
     8701 ttttaactcg tcttccttcc aaaattttag aaaaataaga tcagaagtcg tatccataag
     8761 taaaataaag tttttgcctc atgttgtgta tgcaaaaaac tgatttattg atttatggag
     8821 catcatccta tcttgatgca tggaatcccg ctaaatcctc taaaaaagcc atggagatag
     8881 gtatttaaaa gagtgaggac aatatttctt gatagatcga ataattttcg ttatcataac
     8941 acacgaagat gaccttttga ggaaactcat gagatggtaa gaattggata actgtatgga
     9001 tggcaatttg agctgcatct tttttgggga agccataaat gcccgtactg atattgggga
     9061 aagccacctg cccgaatttg ttttcgaaac tcaggcttaa acatttccga taacatgatg
     9121 ctaaaagatg agcttcgtcg tgcttaccac ctctccaaac tgggcctacg gtatgtatta
     9181 cccaccttgc ctttaagtta aaacctggtg tgattactgc atcacctgtc ggacaaccgt
     9241 ccagtttctt gcaagcctcg agcaagagag ggcctgcggc tcgatgaatt gctccatcta
     9301 ctccgcctcc acctctcaag gaagtattgg ctgcattgac gatggcatct acctcacatc
     9361 gagtaatatc tccctgtgcg acctctatcc tttcttttat tttttctaaa ttttccatgc
     9421 ttaaaatcat tagtttttaa ctttaacata aagctgagca ttttcgtaac gcaccacctc
     9481 cacttcctgt cccttcgaga tatatccatt tatagcaaca gcatcgaata cttcttcttc
     9541 gatcatgatt ttacctgcag gtcgaagtat ggtaagtgca aaaccggttt tcccaaccat
     9601 gctggaataa tcctcattgg caatggtata tccgagttct ttgtcctgag tagtatcgag
     9661 ggccagactt cccaggaaag ggacaggatg actgaagagg cgcttactgc catagaatga
     9721 agagatcaac gaaataaaaa tggctatgat taccgtaaag aatgccttaa gaatatcctg
     9781 aacgatttgg ccggcaggta tggcattgaa atttcccacg aggctgagtg taagaccggc
     9841 taccacgaga ataatcccgc taattcccgt cactccgaag ccgggaatgg caaatatctc
     9901 gatagcaatc aagataagac cgataatgaa aagcaagatt tcccaatgtg ctgccaatcc
     9961 ttccaggtat aatggagcaa aatacaaaat agcaccgagt atggcgattg ccaatggaaa
    10021 gccaatgccc ggggtctgga gttcgaaata gattcctcca attatcagca tgattaaaag
    10081 agccgaaacg atgggatgta tcaagaaatt gatgatttta tccactccgc tcaacttttg
    10141 ggtgatgata acagcatcgt cccatccata ttttttcagt atttgttgaa tattttcagt
    10201 ttctccttcg caaaaaccgt attggatggc ttccgaggtg gtaaaggtaa tgacggttgt
    10261 ggagtcgtct atgccgggga cgacgatacg tgtatccacc atagcctgtg caatttgagg
    10321 atcgcgtccc ttggcttcgg ctgttgaacg catcatcgaa cgcatgtagg actgatattt
    10381 gtcgggtaaa gctccgccag actggtcaac taccgtagca gctccaatat tggctcctgg
    10441 tctcatatat attgaatcgc aggcaatgct gatgagtgca ccagccgaag cagcattatt
    10501 atctatgaaa acccaaacgg gcaatggaga tgtcaaaatt atggtacgta tgctgtcggc
    10561 tgcatccagt gtgcctccgt aagtattcat gtgaataata atcagatcta ctcctttttc
    10621 tctggcttct ccaaatccct tctgtacttt tcgccgcatg ggaggagcaa tctcttcctt
    10681 catttcaata acatagactt tccctacctt ctcgggtaag gtttttttct tgactgtata
    10741 tgtctgctca gccaataaaa ataaacccat gcccaaaaat gtcatgatca aaaataaatg
    10801 tttcattttc gatgatacgg atttcaccac gaaattagtt atatttttta gaaaagccat
    10861 caagggaaaa acatctgaat ttgattcaaa agctgttgat ggaggattcc gttcgagctc
    10921 aggatctggc gtccgaatac atgatgaggt tttccttcga aatcggaaca ttttcctccg
    10981 gctctttcca caatgaagga acctgccgca acgtcccact cgttcaagcc atattcataa
    11041 aatccatcga aacgtccaca ggcaacccag gcaaggtcga gcgcagccga gcccaagcgc
    11101 ctgagacccc gtgtgttctt cataaaatgt ttaaaaagtg ctaaatactg atccagtaaa
    11161 tcatagtcgt aataaggaaa tcctgtggct aataaaattt ctttaacatt cgaacgacgt
    11221 gaaacttgta caggtttacc attgcaaagg gcaggtccat ctttccatgc taagaaactc
    11281 tctcccgaaa aaatttcgag cacttcgcct accacaatct cgctattttg catcaaagca
    11341 atgctcaccg aacagatggg aactccatga atgaaattgg tagttccatc caagggatca
    11401 ataatccaat taaactcctt gcctcttagg ttttgggtat tttcctcggc tataaatcct
    11461 gcattgttaa tcaaaggctt taaatgatcg atgatcaagc tttcggaacc tttatcgata
    11521 taagtaacat agttatgtaa acctttggtt tcgacgtccg attcagataa tgattttctt
    11581 tgctccaaga tgtatcgggc ggcttctttg gcaatacttt ctacctcgaa ggcaatttcc
    11641 ttaaaattca tattcgttgg gtttgtaaat gtttgtattt gcgatagaaa acaatggaga
    11701 acacaacacc agtgatgaaa atggctatgg caatgatttg agcttgactc aattcgatcg
    11761 ggcctatgat atgataattg cggttgacac gaatttcttc gatgaaaaat ctctcgaaag
    11821 cattcaagat taaatagatt ccgaacagta agccgggaat tctgagtttt ttccttattc
    11881 cccataaaac aagaaagaac aaggtgcaaa tgatggtttc gtataaagga gtagggaaaa
    11941 cgggctgttg aagcacgaag caatgctgac cttcgcaacc tgggattgga atcccttcat
    12001 taataacatt atgaggataa ttaaacgccc ataaccaatt gggtagaaat gaaagccaat
    12061 ggggtttggg actagtattg ggaattcccc agcaaccgtc gcccgacagt tggcatccaa
    12121 ttcggccaac ggcataggcc agtattaaag ctggtgctgt tgcatctgca atatgttcga
    12181 atgcaattcc atattttctg gcataaaata ccacggctat cgcagccaga atcaaaccac
    12241 catagaaagt aagaccacta aatgaaaata aagtacctaa aggatcacgg actaactcat
    12301 ccagatgctc catcacatcg aataacttgg ctcctatgat accgaacacc acagctacaa
    12361 ataatatatt gacggttaat tgatgaggat gaatttctac gaatttggtc ttaggtttgg
    12421 gcagcttttt cttttctttg gaatgccaga tgtaataagc agaaaatccg cccgcaagta
    12481 atcctcccaa ccagcttcct ttccatgaga gaatgaattc ttgaggatga tcggcaaaaa
    12541 aattataatc cagaataatg cctatgatct tgaagccaag tatgaatgta atgaaaccag
    12601 acaaagcaat ttcgatagga gtggctggta agccctcgat gatcttttta gtatgaggtt
    12661 ttaaaatccc ctccctttct ttccgcttca attcgctgtt gaggatgagc ccgcctacta
    12721 caaaggcaat tgccatcatc atcccatacg actgaattgg caaattaata tgagttccga
    12781 aaatatcgtt gattagatca ctgattttag gatacatata tgaaaaatga attgaaggat
    12841 gatttatcga ggatagtagg cataatttcc tgtaacattt ctttgttcga gtggtaaatg
    12901 aatgatttga tttacaaaat cttctctgaa aggggtttct acgcgaatat aattttcgga
    12961 ataacccgac atcatgccat cagtgttttc cgattcgaac aacacgttta cccactgatg
    13021 ttggcatagt ttatagaatt ggtcgagttt tgctgctgag tactggtgaa gcatctgact
    13081 cctttggtgt tttatttggg gtgaaacctt gccatcgagg cttgcagcag gtgtgttgga
    13141 gcgttcgctg taagtaaaaa catgcatata cgaaagagga agactttgaa taaactgacg
    13201 ggtttcctcg aaaagctcgt cggtttcgcc atggaagcct acaattatat cgcaggcaat
    13261 acatgcataa ggcattaact gtttaatgta tttaacccga ctctcaaaga ctttggtcgt
    13321 gtattttctc ttcattcttt ttagtattgt atccgagccg gattgcaaag gaatatggaa
    13381 atgtggcatt aaaacccgtg attcagctat taatcgaatg atatcatcgc tcaataactc
    13441 tggctcgatg gacgaaaggc gaactctctt tacaattctt cccttatcga aaagttgcag
    13501 caaatcatat aatgattctc cattttttct accaaaatca ccaatattaa cgcccgtaag
    13561 aacgatttcc tttactccgt ctgctgcggc tttttgcgcc cattgaagag cttcttgtac
    13621 agatggactg cgcgacttac ctcgagcata aggtatggaa caataggaac agaaatggtc
    13681 gcacccatct tgaatcttca gaaaggaacg agtcctatcg cccgaggaat aagcaggatg
    13741 aaactcttgc aagttttttg aaggttctga atctaaagat gtaaccgaaa cctcgaggtt
    13801 tgttgaaaaa tcacgggaaa tcagttcggt aatccgaaat ttttcctgat ttcccaaaac
    13861 ccactgcact ccgctgatcg ctttcaattt ttttgcctcg agctcggagt aacatccaat
    13921 cacagctata aaagcctttg gatttcgccg gtgagcctga tatatggctg cacggctttt
    13981 cttttcagcc accgatgtaa cactgcagga atgtatgata tagatatcgg cttctttatc
    14041 gaaatcagta atttgataac caagttctcg aaatttgcgt atcaaagtgg aggtttctgc
    14101 aaaattcaat ttgcatccca atgtatgaaa agcaactatt attgaattca tgcggcaaaa
    14161 ttaatatatt cctttctatc gattcttctt tattttcact gagcttttct cccttttttc
    14221 tctatatcag gtacatattg ctgcatgatg atacctacta cgaccaaaat caagcctgtg
    14281 atggtactta acatgatcct ttccttcaat ataaaattga tatagaacaa tgaaagaaat
    14341 ggagaaagga aaaccagatt gctgatatga gctgttgttt ttgaatattt taaagctttt
    14401 agccaaaaga tgaaagtgag tcccatttcg aaaattccta cataaatagc tccagccaat
    14461 cccttaatag gagggaaagt caagctatgt ttgatagccg aataaatgat aatgagaatt
    14521 gtaccactta aaaaaccaat aaaaagtttt tcgacctcat cgccagctat cttcatattt
    14581 agaatccaat atattgccca tataaaagga caggtaagag ctagtacaac ccctaaagga
    14641 ttactgaatt gcataagcca aggttttcca tgggtgctga tgatgacaat tcctataaaa
    14701 ctgatgaaaa ctgaaattat gctcatccag cttatttttt gaccaagcat gggaatggaa
    14761 agcaaaacca gaactacagg ccagaaataa tttaaggttc cggcttcttg agctggcagt
    14821 aactcataag cttttaatag aattaaataa taaaggaaag ggtttaatat cccattgaat
    14881 gctaatcgca acagttcttt gtgtggccta tcaaatacat tcctgagtct tttgttgatg
    14941 attaaataaa tggtgaaaaa gagggtagaa gtaagactgg cataaaggag taattctaca
    15001 aaactaacat atctcagagt aatcttgaaa gccgtggcaa tagttgacca ggcaaggata
    15061 gccaatccgg caaaaagata tgctttattc tggcttttca tctggcaaag tttagaaaat
    15121 accttctaaa acttcatcga gcgaggcgcg attaaaataa cgttctattt cgtactcttt
    15181 tagcttttgt ttaacatcac tatattcatg cgggagtcct ttcaaagccg attctatttc
    15241 ggatatctcg tcagaactga aaaagtcgcc aaaaatttta accgatgtta taattccttt
    15301 ttcaaccata aggtcgaatt ctacaaatcc cccagaagtt tttacaattt ttcgaaagtt
    15361 gtattcgggt gagttcccaa aattccattc ccatgtttcg tatttgctat tttgtaaatt
    15421 tgtaatgtag gtaatatctt caggtttgaa ttcataaatc ttgctttcgg gatacatttg
    15481 ggaaatatgc gtgaaaatta aatcacgaaa ctggattata tctatggttt tttttaaata
    15541 ttcactaaca ttggcaaccc gactgcaggt agattttacc gccttgtcct gaaatttaac
    15601 aggatcgacc tttaaggctg aagaaagatt gggcaaatgt gctgaataca atatggtgcc
    15661 atgatgtaaa aaccggtttt tataaacgca ttctgcattt cctgaaattt tctttccatc
    15721 gattacgata tcattccggc cttcaaaacg agcatcgact cctaaattaa ccagaacatc
    15781 cactatgggc tgagtgtatt ttttgaaatt catcaccggc ttgctattgg gtgaattcga
    15841 aatgaaggta aaatttaaat tgcccaagtc gtgaaatact gctcctccac ccgtgatacg
    15901 ccttacaacc tttatatgat tttcttttac gtattcgata ttgatctctg ccagagcgtt
    15961 ttgatgtttt ccaataataa tagaaggctc attcctccaa agcataaaaa catcttcctc
    16021 gtagtttttc agaagatatt cttcgcttgc taaattgaaa taagggttag tattttgatt
    16081 gttaatgcaa aacataatta tttagattaa tttttaataa gcagcgaaat taataaaact
    16141 tcgaaatatt tgagtctaag tcaaaaaact cgtaaataca agaagtaatt gaatgagacc
    16201 ttgataaaac tttgacattc tccgatttta tttggataat tttgtgctcg atattatcat
    16261 tttaagaaat gcttcaaaat tttgaattat gtcgaagtcg ctgagtgaaa ttctgttcga
    16321 agaaggagaa gaacgtgaaa ggtattacaa tgccgtaagc cctccattga tacaaacttc
    16381 caactttgtt ttttcgacgg tagagcaaat gcgtcgtgca ttgagtgatg aatatacaca
    16441 ttcggtatat agcagaggaa ataatccaac tttagccatt ttacgtaata aactggccaa
    16501 acttgagggc gccgaagatg cgctggtttt ttcgagtggc tgcggtgcca tagctgcagc
    16561 tttgtttagc cagttacgcc atggcgatca tctggtttgc attcgaaaac cctataactg
    16621 ggtagcgcat ttttgcagga taattttgcc gaaatatggc attcagacta ctttcgttga
    16681 ggaaggcagt gcgcgagctt atgaagctgc ctgccagccc aataccaaat tattttatct
    16741 tgagtcgccc aatactttta cgttcgagat aaccgatatc agagccgtag ccgaaatagc
    16801 caaatcgaaa ggcattgtga cagtcatcga caatagttat tctactcctc tttatcagtc
    16861 gcctttatcc ttagggattg accttgtatt gcattcggca tcgaaatatc ttgcgggtca
    16921 tagtgatgta atagctggag ttttagcggg tagcaaggaa atgattggca aaatttataa
    16981 ttcggaatat ctggcctttg gtgcagtgtt acctccattc gaggcttggt tgatgctcag
    17041 gggcttacga accctcgaga ttcgccttca gcgaagcagt tccaatgcta aagcacttat
    17101 cgaggccatc gaaaaccatc ctcgcataaa aaggattctt tatccttttc attcgcaaca
    17161 tcctaaaaac gaaatcgctc gaagccagat gaaagattgc agcggtttgt tttcgatcga
    17221 attggaggca gatactcccg atgaagtaat tttgttttgc aatagtttaa agcatttttt
    17281 gaaagcggtt tcgtggggag gattcgaatc tttggtcgat cctgctatca ccgtttataa
    17341 ttttgaccat cctgaattaa atgagctctc ctggcgtact gtgcgctttt attgcggatt
    17401 ggaatcacct gaaatattaa tagatgatgt attacaggcc ttgaatattc ttgataatgg
    17461 agcaacaaac aggcaaaagt agaatggcaa ggctgctaaa aagcttcgag tatgcaggcc
    17521 gaggcctctt ggagatgata agaagtcaga tgaatgcgcg aattgaatta tttatactca
    17581 ccgttgtgat gattgcaggt tttgtcttca agattcgggt caatgaatgg ataacgataa
    17641 tactcgtttc aggcttggtt ttagcggccg aggcattcaa tagcgctata gaaaatctgg
    17701 ccaatgcgat atcgttacgc cagttcgatt ccaagattaa aaagataaaa gaccttgcag
    17761 ccagtggggt tttaatttct gctgtttgtg catttgttat aggtttgatt atttttattc
    17821 cttatattgt tcggtttttt gtatccttat gattttaatc gttgatagtg gagctacgaa
    17881 ggccgaatgg cgtttattgc tcgagaatgg ggatgtgcaa gctgcaaata ccacgggaat
    17941 caatccttat tttattcggt cggaatctat tggtaatatt ctgatgaatg aattgcctga
    18001 taattggatt catgaaagta aaatcgggaa gatttattac tatggtgccg gatgcgggag
    18061 tgagatacaa gaaaaaagta tcgaaaaagc tttgaaagcc atatttccgg aggcgaattt
    18121 gttcatttct tccgaccttt taggagcggc aagagctttg tggcagaggg attctggtta
    18181 taccgctatt ttaggtacgg gttccaatgc aggatatttt aatggtcagc aaatcgagaa
    18241 agggataaaa tcgttgggtt ttatcctggg tgatgaaggc agtggcgctg atttggggaa
    18301 acgcttgctc aaaagatatt tagaaggaaa attcaaccaa gatttagcag aaaattttta
    18361 ccagcaattt cccttcgacc ttgctgattt tttagatgcc atctattcga aaccttttcc
    18421 taatcgtttc atggcagggt ttgttccatt tatgaaggaa aactcgaacg aaatagaaat
    18481 ccagttgatt gtggaggaag cattcgatga tttttttgga ttggtgcaaa cgatagcaca
    18541 atcttctttt cgaaagtcga tacgattcac cggttcggtg gcctgggctt tcgaggaaat
    18601 tttactcgga gttgctgcgc aacatcattt cgaggtgtca ttggtagtaa aaaaccctat
    18661 cgatcttttg gtaaaatttc atcatgctca gccataaata tcaggcattt ctttttaaaa
    18721 gagtagaaaa ccaatcttta tgatatgatt ttacatagag acgcctcgat tgaataagat
    18781 cttcgatttc gagatgcata ttatggttta cttgcaatgc catggggaga ataaactttt
    18841 cgtaaagatt cagattggtt ttaattttat tcaaggaaat attgtcgata tgaagatcgg
    18901 taagattgct gattacgtcg gcacatttca atattttagc ttttttggag ccctggtgga
    18961 gcaacctttc ataaaattct ctttttgatt ctggaaagtg tcgcgttact tccttaacaa
    19021 gccgtatcac ttcttccacg tcttcgtctt gaatctcgcg tatattttct tcccttgctt
    19081 catgaaaatc ttcaagaaga tcgtgaagta tggcagcttt taaaagtaca gaatcgatgt
    19141 aatgataatc gatgaggata ctcatggtag ccatcgcatg cctgaactga ttccctccag
    19201 ttttacgggc tgatgctaat caaggcatag gctttttgaa tatatggggc gagcacaata
    19261 gatgcaagcc tatttttctc atcgttattc atgaattttt tctttgtttc tgataggaga
    19321 aagtactctt caatgttctg aagatgaatg aataaaaaaa gatttaaata gataaaattt
    19381 tttatttcta taacttatct acaaaaaatc tgccatataa tcgatttgtt acatatggtt
    19441 tagaatcagc attcgatata gggaaagggt cgtcgacgca gattgagata aattccgaaa
    19501 tcgaatctgc gattttatca tcttatcttt taaaaggcga tcgaatcatt ccgcttggtt
    19561 atttcaaaga gatgataaaa tatactggta taaaaaaaca ggagaatgcc tatctataaa
    19621 cattctcctg tttttattgt gggagctata ggagtcgaac ctatgaccct ctgcttgtag
    19681 ggcagatgct ctaaaccaac tgagctaagc tccccttcga tattgcgctg caaagataga
    19741 gcataatctt cgttgtttcc aaattttttc gatataattc ttggaatttc cttctatggg
    19801 atgagatttt ttgttaatta gaagaatatc aaattattta tatatcgccc ttcgggttgg
    19861 atttcattat gccataattt ctatctttgc actgttacct tgcaagttgt gtacgataaa
    19921 tatttcatcg gatatatatt actgattgtg attttatcgg gctgtaatcc aaccaaaaaa
    19981 ctacccgaga ggaagtttct acttaccgaa aacaaaattg agatagagcc tgaaaagcag
    20041 atgaaggcat ctgaaataca gaaatatatt cttcaaactc ccaataagaa gtttctggga
    20101 atgtttcata tcaatctatg gctttataat gaaacggcaa attggaaggc atcgaagttt
    20161 cgtgactggt taaggaggtt aggagggaaa ccaccggcta tttacgaccc taatcttacc
    20221 ttgtcgagcg aaagaaacat caaaaattat ctttacaaca agggatattt caatgcaact
    20281 ataaaaagcc aagaaattct gcaagacaaa aaagctgaag tagtttatca tataaaacta
    20341 aataaatcat tcaaaatacg gaattttctc ttagaggcag aagatacact tgtaagccgt
    20401 tatgcgaatc gaatcatggc taacaccccc attcgtccag gagaaatcta cgattattat
    20461 gtattggaag gggaaaggga acgactaact gttgaaatgc gaaatgcagg attttatttt
    20521 ttcaccaacg attacatttt ctacgaaatt gatactacca tagggcaaaa aaatctggat
    20581 ataagaatgt tgattttgaa tccatcaaat tttacgggtt ttgatcctgg ccgagccaac
    20641 gaatggttgg ctttgcatca cttgtacaag attcgaaata tttatgtgtt tccacaatac
    20701 agtagccact ccgaaacaaa ttcattttct gacaccttag tctataatcg tacaggaacg
    20761 gatgatacaa gtcaggcgaa aaatgatata ctatacctta tatataatga acctctgaaa
    20821 attcagccaa aaattatttc tcaggctatt tatatacatt ctggtgactt gtactgctta
    20881 aggaatgctg atttgagttt tcgggcatta gccgagttcc ccatttatcg ttctatcaat
    20941 atcgaattca ggctggccga tacagctggt atttccctga cgcgacatgg ccatctcgat
    21001 tgttatatct acttgtcgcg agctgaagta cagggattca ctatcgaaac agaagttacc
    21061 aatacgggag gtgatctggg attgtcgggt aatttggttt ataccaatcg aaatgttttt
    21121 cgaggagccg agatgctaag gctaaaactg agaggtgcag ccgaaatgca aaaatcgttc
    21181 gagcaaactt cacagaataa gcttcttctg ttcaatactt atgaatcagg agccgaactc
    21241 aatctgactt tcccgcgttt tgtttggcct ttgaaaggat tacgttttcc cagttccatg
    21301 cgaactaagt cgaatcttct ggttggatac aattatcaaa accgacccga ttatattcgc
    21361 catattacta atgctgcctt ttcttatcaa ttgaaagtat ccgattttaa gagtcatctt
    21421 ttaacaccct tagaattgaa cgctataaaa atatatgcaa ctccttcatt ccagcaactc
    21481 atcgattcga tcgacgatca aggcattcgg agtcaatata gtgatcatct gataccagcc
    21541 ttgagataca gctatatttt taccaatcag gaaattggta aattccacaa ttacaaatat
    21601 ttccgtttcg acttcgaatc atcgggcaat accctttacg ctgtggatcg attaaccaat
    21661 gtatcaccga atattcataa cgaatatacc ttcttgggaa ttgcctatgc acaatatttg
    21721 aaagccagtt ccgattttcg gtatttttat atgtttaact tgaataatac cttggttatg
    21781 cgaatttttg ccggggctgc tattccctat ggtaatagta attatcttcc cttcgaaaag
    21841 ggcttctttg ctggcggggc aaatggcatg aggggatgga cgcttcgaat gctgggacct
    21901 ggccattttt caagcgaagg tggtcaaaga tataaccgca tgggcgatct ccatttcgaa
    21961 accaatctcg aatatcgttt ccctatgtat aatttcttta aaggtgcatt ctttctggat
    22021 gcaggaaata tttggctggt aaaatccaat ccaatgtatc cgggaggcct gttaaatgcc
    22081 agaacttttc tcaaggaaat tgccatggat gctggtatag gtatccgttt agattttact
    22141 tatttcgttt ttcgtttgga cgctgcgttg cctcttctcg atcctgcaag gccagaggga
    22201 tcacgatggc tactgccaaa atctcgttta tcggatctgg tatggaattt tgggataggt
    22261 tatcctttct aaatgcacgt gttctgttaa aacgattatt gaatacatga cttatactga
    22321 agcggtagat tatatttttc aacatttgcc tatgtttaca aaaataggag gtgttgccta
    22381 taaagccgat ttgagccgca ctctttattt gtgtgaaaag ctcggtaatc ctcatctaaa
    22441 ctttcgttcc attcatattg caggtacaaa tggaaaaggc agcgtggcct cttttctaag
    22501 ttccatcttc atggagtcgg gttcgaaaac agctttgttt acctcccctc atctgcgcga
    22561 ttttagagaa agaattacca taaatggaat actcatcccc ggagattatg tagctgaatt
    22621 catcgaagaa aacagaccaa atttcgaaac cattcaacct tcattttttg aaatgagttt
    22681 tgctcttgct gtcaaatggt ttagcgacca aaaggtagat gtggccatca tagaaaccgg
    22741 aatgggaggc aggctcgact caaccaacgt aatccttcct gagataagca tcataaccaa
    22801 tattggacta gaccatcagc aatttttagg ctcgacactt gctgagatag ctgccgaaaa
    22861 ggccggaatc atcaaaccaa acattcccgt ggtgataggc gagcataatc agcaaaccga
    22921 tcgtgttttc atcgaaaaag ctatccaaac tcattcatta ctcttttggg ctgaagattt
    22981 aagttctgtc gaagattcct tctatgatgt tgagaataat tacttgaacc tttcgctgat
    23041 gatcctttcg ggaaaactta tccagctgca aagccctctt ccggctgagt acgaaattaa
    23101 aaacataaga accgtgatgg ccgctgccga agttttaaac caaagcggat attccctttc
    23161 cgatgaaatt ctggctaatg gaataaaaaa tgtgctgaaa aacactggtt tgaaaggccg
    23221 ttggcaaatt ctatccaaaa atcctctcat tgttgctgat atttctcaca acgaacccgg
    23281 cataaaggaa cttatgaagc aattgaataa attgaaatac aatcatttgc atttcgtatt
    23341 aggaatggtt tccgataagg acagtttttc tattttgcag ctttttccca aagaggctac
    23401 ctattatttt tgccaaccca atattcctcg gggcaaagat gtcgaacaac tggccgatga
    23461 agctcatgaa tttggtttgc agggcaaaat attccacagc gtagaagaag ctttccggac
    23521 tgcttgcgct tcagccctgg ccgacgatat ggtgctgata agcggcagtg cctttgtagt
    23581 ggctgaaatc atctaaagta aaggctattt cgcttttctt atcaattccc tcttgtttcg
    23641 ttcaccgaaa cttttcatct gaactgaaaa tttgtaggtt tgccagacaa tatgctttat
    23701 gataagtatt cgaatgaatg agaatctatc atgcattaga caaggatttt taatcttatt
    23761 ggtcagtaag atgaaagcct atttaatttc gaattttatc ccatgatact gaattatatc
    23821 tggttcggtt tctttattat cgccttcgtc atagctctgg ttcatctaat ttttttccaa
    23881 gattccagtg tatttgatgc tattctgcaa tctacgttcg ataatgccaa ggtaggtttt
    23941 gaattatcgc ttggtttgac tggagtgatg actctgtggc ttggtgtgat gcgaattgga
    24001 gaaaaggggg gtatggtcga gattttagcc aaactcataa atccactttt caggaggctt
    24061 tttcctgaaa taccggctaa tcatccggta aatggatcta tattattgaa tatagccgct
    24121 aacatgcttg gtctagacaa tgcagccaca cctctgggac taaaagccat gaaccagctt
    24181 caggaattaa atcctgaaaa ggaaaaggct tccaatgcca tgattatgtt tctggttatc
    24241 aatacttcag gcttaaccat tatccccgtg agcataatgg tatatcgagc tcaattgggc
    24301 gccgcaaatc ctgccgatgt tttccttcca attctgctat ctaccttcgt ctctaccttg
    24361 gcaggaatca tttcggtcag tctggtacaa aggattaatc ttgttcaaaa aaccgtgcta
    24421 ttatatttgc ttggattcac tgccattgtt ggaggattgg tgtggtattt gagcattgtc
    24481 gatcaacaaa ccatgaataa aatttcgaca gcaatcagta gcgttattct attttcgatt
    24541 atcattattt ttatggtaat tgctacggta aaaaaggtaa gggtgtacga tgaatttatc
    24601 gaaggagcca aggagggttt tcatattgca gtaagaatca tcccttatct ggtgggaatg
    24661 ttagtagcca ttggtgtatt tagggcatcg ggagctatgg atagtctggt cgatggaatt
    24721 tcgtggtgtg cacaaaaggc aggtctcgat acttcattcg taccggcact cccaactgct
    24781 attatgaaac ctctgagtgg tagtggagca agaggaatga tgctcgaagc catgaaatat
    24841 tttggtgccg attcttttgt tggaaggtta tcatgtcttt ttcagggatc aaccgatact
    24901 acgttttata ttatagcact gtattttggt tcggttggaa tacgcaaca
//
LOCUS       DAOORU010000014        24346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_2499_length_24346_cov_5.347413, whole genome shotgun sequence.
ACCESSION   DAOORU010000014 DAOORU010000000
VERSION     DAOORU010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24346
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2499_length_24346_cov_5.347413"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="PLF20_02060"
     CDS             complement(<1..550)
                     /locus_tag="PLF20_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX79826.1"
                     /translation="MKHFLSIFLIALVVISACKSNSSKKELPKENLTATIHDTTQKSY
                     QPGQDAFKPGTSDTDENSQWTFTKAATLIGLGNYDEALNYMNQYIEKNPEDGNGFFVR
                     GFIYEQKKEYEKAIEEFDEALRLNAAHTYAYMYKGEAHIYLKQFKEAYVCFTKVIQIE
                     PKNMYAYYNRGIALSNMNKYKEA"
     gene            complement(580..1506)
                     /locus_tag="PLF20_02065"
     CDS             complement(580..1506)
                     /locus_tag="PLF20_02065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX79827.1"
                     /translation="MKQYFKIILLLFISWNLIIESSAQNDAEYFIKQGITAARLGYTE
                     QALQNFNTAIYKDPTLPEAWYNRGVLYYKLGNFQKALFDMDQAARINSKQTNVFYQRG
                     LIYHALGNDSLAREDFRKTISLDTTMTDALFNLGLIDFEHQNYEQAFMNFDKLVKSDS
                     KNTQALYHRGLCKHHLGRFFEAIADFDAALALLPNDAEIILSRGVAKAEAGYYYTAIE
                     DFTRVIYLNPNYAEAYFNRAMASFNLYKFAEAESDYNMAYSIDSSAEALTGRGLSRLA
                     QHQMDTGCADLKMAIELGYEPAIKYLMNYCKD"
     gene            complement(1503..2624)
                     /gene="lpxB"
                     /locus_tag="PLF20_02070"
     CDS             complement(1503..2624)
                     /gene="lpxB"
                     /locus_tag="PLF20_02070"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOX79828.1"
                     /translation="MKYYLIVGEASGDLHASNLVKAIQELDSEAEFRGFGGDLMKKAG
                     VNIIKHHRDLSFMGFVEVAGHLPTIIKILNECKSDILNFQPHALILVDYPGFNLRIAK
                     FARKNNIKVIYYVSPQVWAWHQSRVRSIKKYVNRMITILPFESDFYSQHHFEVFYAGH
                     PLLDVISEYIPPSREEFLSRNSLNHQPIIAILPGSRKQEIKVMLPVMLSMVKRFPEFQ
                     FVIGAAPSLPDNFFEPITNHHRIAITKGQTYDLLSHSYAALVTSGTASLETALLNVPQ
                     VICYKGQPLSYFIARRMVKIGFIGLPNLIAGRQVVKELIQNYFTPARLYDEIDKIVKD
                     ERYRNEIRKGYDEIRHLLGGPGASKRAANEIVRVIASQS"
     gene            complement(2621..2911)
                     /locus_tag="PLF20_02075"
     CDS             complement(2621..2911)
                     /locus_tag="PLF20_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79829.1"
                     /translation="MNKNTYWFGLLAGLLGMITGGILFWLIGLLLTAITGLDPFFQPW
                     QMYWLSLIIPIVLIRHFFMRRKFERTGRGVLTMVFVLVLGYFIYVRIKAGTI"
     gene            complement(2904..3674)
                     /gene="surE"
                     /locus_tag="PLF20_02080"
     CDS             complement(2904..3674)
                     /gene="surE"
                     /locus_tag="PLF20_02080"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOX79830.1"
                     /translation="MDNKLILVTNDDGVNAPGIKALIRMMRPMGHVIVAAPDKPQSGM
                     AHSITVNIPLRYQKISEEENYEEYGISGTPVDCVKLAEKFLVPRKPDLLVSGINHGSN
                     ASINILYSGTMAAVLEGCMLDIPSIGFSFCDYSYHANFSCLDEWVTRIARIILENGLP
                     RGVCLNVNIPETGGEPIRGIHITHQGEGRWEEEFDHRLDPRERDYFWIMGNFINYDGQ
                     PGSDLCALENNFISVMPVQYDMTAHYALDYLKKIFNNE"
     gene            complement(3910..4491)
                     /locus_tag="PLF20_02085"
     CDS             complement(3910..4491)
                     /locus_tag="PLF20_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008774130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOX79831.1"
                     /translation="MLSNLQDYILILASRSPRRQQLLAGLDLPFLVFTKETPEDYPLD
                     MHGKDVALMLARRKSLAFNNEDLPENFLLITADTIVWLNNHILNKPSSRQEAIIMLKQ
                     LSNNTHFVYTGICLRSSRKTETFVAESEVRFRKLEKEEIEYYVDYYSPYDKAGSYGVQ
                     EWIGYIGIEAIHGSFYNVMGLPTQILYKHLQTF"
     gene            complement(4586..6223)
                     /locus_tag="PLF20_02090"
     CDS             complement(4586..6223)
                     /locus_tag="PLF20_02090"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOX79832.1"
                     /translation="MKIALIQLNYIIGDFEGNTKKIIQSIQEAKSKGASLAIFSELSV
                     CGYPPKDLLEFDDFIEKCEESVQKISRACRDIAAIVGSPIKNETRKGKPLFNAALLLY
                     EGQVLGIQRKTLLPDDDIFDEYRYFEPNQQFDVFEFRGERIALCICEDLLDTDNNPLY
                     LCKPQDELLKLNPSLMVNISASPFHDNQTELRPKILKENTVRSQIPLIYVNQVGAHTD
                     LIFDGDSMVIDIYGDIVARMKLFAEDMVIVDTKKLHSISNALPHYPLSRIEMIHDALV
                     FGIQEYFGKLNLKNAILGLSGGIDSALVLVLAKEALGNDHVKALILPSHYTAPQSITD
                     AILLSQRLEVDFEIISIDKAYKSLENTFEYFFKKMPSDVTEENLQARLRGIILMAFSN
                     KFGHILLNTSNKSELAVGYGTLYGDMCGGLSVLGDVYKTDVYALAHYINCKSPIIPES
                     TLLKPPSAELRPNQKDSDSLPLYEILDPILRLYIEEQQGPTEIIAQGHDSALVLRILK
                     MVNSNEYKRYQAPPVLRVSKKAFGSGRRMPIVGKYLE"
     gene            complement(6249..6971)
                     /locus_tag="PLF20_02095"
     CDS             complement(6249..6971)
                     /locus_tag="PLF20_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOX79833.1"
                     /translation="MKKFIGLLLVMVAALNIFGQQSEKKYQFGIKVSPNYTWINPETR
                     NYKNDGGKIGFTWGLIADINLRDNYFFSSGFGLSYQGGKICYPVNYPIPKPKTNGIGD
                     TTLILGKVYRNYSYRYLEIPLLFKMKTLPKKNTRFYGDMGLVPAFKLRAHANDIFYSQ
                     QGTTLKNEPDVSSALNAMKLSIFVGGGFEYHFDQSTFIMIGLGFNNSFTDIFSDYNSH
                     PSVNASEDGIANYLELSLALFF"
     gene            7381..9129
                     /locus_tag="PLF20_02100"
     CDS             7381..9129
                     /locus_tag="PLF20_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HOX79834.1"
                     /translation="MTQVRVKVWSDEFNYSGLPDTNLWGYDVGAGGWGNNESQYYTDH
                     RWLNARAESGKLIIEARKENFEDCEYTSARLVTRNKGDWKYGRIEVSARLPHGRGTWP
                     AIWMLPTDWVYGSWVKSGEIDIMEYVGSDPGIVHGTIHTQAYNHIIGTQKTDTFYVPD
                     AETNFHLYAIEWTEEKIDFFVDDEKYFSFYNEHTGYTTWPFDQRFHLLLNIAVGGSWG
                     GQQGIDPDIWPQKMWVDYVRVFQNIPFEEIHIEGPCCLQAGQANAVFEIQDIEGAIFH
                     WTVPEGATIESGQGTHKIKVTWGNDEENVKCEVIHPHAGGIYSKEVFIALAPVYSRYD
                     LTNFAIQDTEGWTITANPPNQVNIFKQDTLMQLNYSIFPNAGKPYLEYTFPTIVDMNQ
                     HPHLNVFLKTFNKSGTAATKIELFDSRKRFTDVDPGFSILPLQLSGEIQAFHFDFTNH
                     WNTNLPFPNSKVDSNCISGLRIYLDTPGSECPCFDSLWISRIEISKENYLGISNRQSE
                     PFIRFYPNPFRQTLYIKSSKNFPDHFYIFDLTGREVFHTIINQPVNLLSLDFLNPGVY
                     LGVLKNSRYRQYIIKQ"
     gene            9499..12447
                     /gene="dnaE"
                     /locus_tag="PLF20_02105"
     CDS             9499..12447
                     /gene="dnaE"
                     /locus_tag="PLF20_02105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOX79835.1"
                     /translation="MLRIAHSYYSLRYGTLSVEDLVELGAEAGLKRLVLADINNTVGL
                     PDFVKACHRADILPIAGMDIFKENHRLYTLIARNANGFREMNEFISQYNSQQIPYPDE
                     APEMKEVIVIYPFSKAFTRPFSPHEFIGIRPSELNRLIFSPLRNQVDHLLAIQYITFA
                     NDEGYEIHRNFRAIDNNILLSKLSSDLLAAKDERLLVPEASQNFLRDHEALVQHTERV
                     SESWQIDFDFMTSKNKKTFTGNREDDKVLLEKLALEGMVYRYGKGNAEAEQRVRSELE
                     IIHRLGFSAYFLIAWDIIRYSMSLGFYHVGRGSGANSVVAYCLRITDVDPIELNLYFE
                     RFINPKRSSPPDFDIDYSWKDRDTVIDYIFKRYGRQHTALLGSMSTFRDRSIYRELGK
                     VYGLPKHEIDELAEHPEFRIQRDHIARHIHEIACKIADFPNIRSIHAGGILISEEPIT
                     AYTALDLPPKGFLTTQWDMYVAESLGFEKLDILSQRGIGHIKECADIVLQNRGLKIDV
                     HQVDQFKADPLVREKIRNGETMGCFYIESPAMRGLLKKLACDNYLSLVAASSIIRPGV
                     ARSGMMREYIYRFHHPNEFSYIHPIMKEQLAETYGVMVYQEDVLKVCHHFAGLDLADA
                     DVLRRAMSGKFRGKKEMARIVDRFFSNCRERGYPEDIIAEVWRQIASFAGYSFSKAHS
                     ASYAVESYQSLYLKAHFPLEFMVAVINNFGGFYSTWVYFNEARRYGARIELPCVNKSR
                     VVTSIEGNTIYIGFIHIENLERDFSQLIVSERERNGLYLDLADFIERTQAKLEQTLLL
                     IRTGALRFTGKTKANLMWEAHIFLNKKNSQASSQPPLFKISARPFQLPALPSNSLEDA
                     YDEIELLGFPVTVSYFDMLQTRFRGEVISRNMIQHVGKTVRMLGYLVTLKYVRTVKNE
                     LMHFGTFVDAEGELFETVHFPNSLKQYPFHGKAMYLLAGTITEEFQHPSLTITKMAKL
                     PFQPDPRHKN"
     gene            complement(12891..14282)
                     /locus_tag="PLF20_02110"
     CDS             complement(12891..14282)
                     /locus_tag="PLF20_02110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX79836.1"
                     /translation="MSEKELPTRHRNLILTLILITSFANPFMGSSINLALPAIGSHFG
                     LHAVMMSWVAMSFLLSSAVLMVPLGKIADIIGRKRVFLSGTILFSLSSIFCAMSPSVG
                     LLLASRVLQGAGGAMMFGTNMAIITDIFPANERGKAIGTNVMAVYLGLSLGPLIAGFL
                     VKNWGWQSIFYLTAIPVAFVAISMPLLVKTEWKSPHSEQFDWKGGILYIIAMSAFMYG
                     FSHLPNFWAIILAFISLIGLYFFVKIEIQSDFPVFDMRLLSHNRLFAMSNLAALLNYA
                     ITFAITFMLSLYLQYVKGLNPRDAGLILVIQPAMMVIVSYFSGRMSDVYDPRILASAG
                     MSTITLGLMALIPLGTNSSLIYLMVILGVIGIGFGLFTSPNTNAIMGSVEKKYLGVAS
                     ATVGTMRLTGQMISMGIATLVINIFLGNSKIHAGNLSLFLHSQRTTFIIFVILGILGV
                     WASMARKSENLAK"
     gene            14527..15747
                     /locus_tag="PLF20_02115"
     CDS             14527..15747
                     /locus_tag="PLF20_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOX79837.1"
                     /translation="MNSKTSSKIYVAMPVMAEYEYLPTLLECLENQSFQNFKLVVCVN
                     QPDDYRFNPNKIHIYEDNQKCLSLLKSSSYSFDIEIIDRSSTDMGWKGKHCGVGQARK
                     TVMDAIAREAKNNDDIIVSLDADTLFGTDYLEVIAQTFLDYSYIAALSVPYYHPLTQD
                     EITNRCILRYEIYMRVYALNMLFIHNPYAFTAIGSAMALRVDIYRKVGGITPHRAGED
                     FYFLQKLRKHGSVLTWCPAKIYPAARFSDRVIFGTGPAMIRGREGHWEAYPIFHHSLF
                     DTVKITFEAFSRLYLEKIKTPMDVFLEDIFRTSDIWGPLRKNSSEIKSFVKACTQKID
                     ALRILQFLKKYHPQIKKSDEICLKEFMMHYFPESHPALIESFDTCNINALQEIRDFLT
                     MKEYSYRQHHPLLQ"
     gene            15770..16687
                     /gene="miaA"
                     /locus_tag="PLF20_02120"
     CDS             15770..16687
                     /gene="miaA"
                     /locus_tag="PLF20_02120"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOX79838.1"
                     /translation="MITILGPTAVGKTRISAMIAARMDAEIISADSRQVFRGMDLGTG
                     KDLQDYFVDGSFIPYHLIDIRQPGEEYSVWDFSCDFKKAYLEIRSRGRQVILCGGTGL
                     YIEAVIGGYYMPQVPEDKNLRNSLEKESIENLITRLASLRPLHNTTDITDRARLIRAI
                     EIETWKLSHPREETFVPPNFILGITLPREELRMRITQRLHKRIEQGMIEEVEQLKKLG
                     ISHENLDSYGLEYRFISRYLKGQMSKNEMVESLNTAIHQFAKRQMTWFRRMERKGFRI
                     HWLDGMQLAEEVVKKAMQVLDSHPDQWKE"
     gene            16705..17712
                     /locus_tag="PLF20_02125"
     CDS             16705..17712
                     /locus_tag="PLF20_02125"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOX79839.1"
                     /translation="MNERKNIIITGATGFIGSFLTEIGVEKGWQVTAWVRETSNTEHL
                     PSGVNLIKAGFSNRQKMMEALQMIERQNGSIDYIVHNAGVTKTTHPEDFDRVNAENTG
                     RFIEAIQESGISIAKFILMSSLAALGPGNPQTLEPLHPDDVPKPDTLYGKSKLKAEKI
                     VRQSSLPWNIMRPTGVYGPRETDYYLMLRTIKKGLAPVTGCKPHFLSFVYVKDLVKAV
                     FLALETPITQQTFHVSDGKTYTDREYRDLCCKILNTNALKVTVPHWVLKAVSFISETF
                     ARPFKITPTLNKDKYLTMRAINWKSDISNTIEMLHYSPEYDLQRGLDESIQWYRKEGW
                     L"
     gene            17959..19473
                     /locus_tag="PLF20_02130"
     CDS             17959..19473
                     /locus_tag="PLF20_02130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX79840.1"
                     /translation="MNLYSRKRKWKWLLGLFALLIFGSTIFYTDHLVRQIKEADQKNL
                     RIWADAVNRKAALVNYTDNFFRQIQEEERRRVELLAEAYKRLIVTDDQADLTFYLNML
                     EKNKTIPVLLTDENYNILSATNIDIDLSTTKKLEGALLQEFTKYPPIEVPYMKGKKNF
                     LFYTDSRLFSELHEVLNDLNQSFISEVVLNSASVPALIIDSTKSQILAFGNIDSILLA
                     DSASRNKIIQELSVANVPIPITLSEHGKCLVIYRNSFLMNQLKYYPFIQLGIIGLFLL
                     VAYSLFSSARKSEEDQVWVGMAKETAHQLGTPMSSMLAWMELMRMDGLEHPAISEMEK
                     DLIRLETITERFSKIGSQPYLEAANILEVISETFDYLEPRTSRKIQFRVNNQTNKDEL
                     YIPLNVPLFEWVIENLVKNAIDAMGGVGTFTITIQEDHKHVILDFTDTGKGIPRSQQR
                     VIFKPGFTNKKRGWGLGLSLARRIVHSYHKGRIFVKSSQPNVGTTFRVILNKKK"
     gene            19470..20612
                     /gene="hemW"
                     /locus_tag="PLF20_02135"
     CDS             19470..20612
                     /gene="hemW"
                     /locus_tag="PLF20_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOX79841.1"
                     /translation="MTGLYIHIPFCKSKCLYCNFYAVASKKLAQGFVEALLQEIETRS
                     HQWKDPHLRTVYMGGGTPSLLLPGDIENILDFIKRYFILEPSCEITLEANPDDISMQN
                     LESWSKAGINRLSIGIQSQNETILKFLGRHHTAEQALEAYAMARKAGFNNISVDLIYG
                     IQGLNNEEWENALLKILSLEPEHFSCYALTLEEKTPYDFQVKKGKLSLPDEELVAQQY
                     EILQEISSHKGYIQYEISNFCLPGYYSRHNTAYWQGIPYLGLGPSAHSYDGIKRWWNP
                     SCIEEWKKLTTLDIGACPCEILTSINQYNEYIITSLRTSWGLSISHINKRFGEKFTRH
                     TLQIANSLTSIYLKKIGTDRIAIKSQAYILADCILERFILMDDSKI"
     gene            20741..21547
                     /locus_tag="PLF20_02140"
     CDS             20741..21547
                     /locus_tag="PLF20_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79842.1"
                     /translation="MHIYRFRITSDSIEDFVREIDISPNQTFEDFQRVFLQAAPLSAE
                     EKTYFYKANARWVKQYEIAEQPLLSISKEKEGDDVRLEEHRLKIPYTHISLARFRDFV
                     DDPHQKFIIEYRGPDIYLFFMEMVKILETDSLANYPKCVTQKGEIPVKPIIIPEEIQE
                     KTRKQREERLTITSLNDDIPEEMIEEQMKELMEDEVFGAIMSGKEVPPSSNISRKMTA
                     REIAEEFGPEFADMMSTDADEKFDDEDITDEDIDDEFADDKSGEDFDDDF"
     gene            21578..22495
                     /gene="miaA"
                     /locus_tag="PLF20_02145"
     CDS             21578..22495
                     /gene="miaA"
                     /locus_tag="PLF20_02145"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOX79843.1"
                     /translation="MKLQKPLLVVIAGPTASGKTELAIELACKYNTEIISADSRQFYK
                     ELPIGSATPSEEQLAKVKHYFIGNRTIHHPCNVAEYEEEVIALLSRLFTSQPMVIMAG
                     GSGLYIDAVCNGLDILPEPDLLLRHHLHALYHEQGIEALRTQLRLLDTEFYHQVDLKN
                     PIRLMRAIEVCMLSGQPYSSLRTGKSKLRSFHILKLALDVPKPLLIERINHRVDEMMQ
                     RGLLEEAQRLLPFRNLQPLRSLGYKELFAYMEGQFTLEKAIEKIKINTRKFAKRQITW
                     FKKDPLYHWIPPNDLQHIDQLIENALQQS"
     gene            22629..>24346
                     /locus_tag="PLF20_02150"
     CDS             22629..>24346
                     /locus_tag="PLF20_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HOX79844.1"
                     /translation="MKKIIINLLRHLTFWLLIFASGRIFFLIYYHGIIAHSHIKFTEI
                     AGLFFHALRLDVATFCYIASLFLFLYVIESITKWRAINQIVKYLNFLLIGIYFLIVAG
                     ETGLYDEWHTKLNMKALAYLKHPAEVIASAQTLTLVIMILFWLVSVFTACWLYNKFIF
                     QASTKQSSPKAWKAVITYIVLQPILIIGSRGGIQQIPINQSQSYFSKHAILNHAAVNP
                     AFNLYISWFENRQFKDRNPFIYMDQAEARHIVDSLFAKPTTNGMDILTIYKPNIVMLI
                     LESWSADLIESLGGEPGITPEFRKLEMEGILFTQAYAGGKRSEQGLAAIFSGFPPHPY
                     TSITVQPDKYQHLPSIVKKINAQGYHSSFYFGGQLIYGNIKSYIIFNQFDRIKEIYDF
                     KLPNVKGKLGIHDEYTLNELLHDLAVEPAPFFSALFTLSSHSPYDQPMEEKLFWGDNE
                     RNYINSAYYTDWCLGKFFEQARHQIWYPNTLFILVADHSHNSYRNWHPNSFEYNRIPI
                     LFYGDVIREDLRGTQIDWLVSQTDLGTTLLEILKISTHEFYWGRNMFHSGFHEFTYLT
                     FDYGVG"
BASE COUNT         7385 a         4812 c         4840 g         7309 t
ORIGIN      
        1 tggcttcttt gtatttattc atattactca atgcaattcc tctattataa taggcataca
       61 tattcttagg ctcgatctgg atgactttcg taaaacaaac ataagcttcc ttgaattgct
      121 tcaagtatat atgtgcctcg cctttgtaca tataggcgta ggtatgggca gcattcaatc
      181 ttaaagcttc atcgaattcc tctatggctt tctcatattc ttttttttgt tcgtaaataa
      241 atccacgaac gaagaatcca tttccatctt ctggattttt ttcaatatat tgattcatat
      301 aattcaaggc ttcatcataa ttacccaagc caatcaaggt agctgccttg gtaaatgtcc
      361 attgggaatt ttcatcggtg tcagacgtac ctggtttaaa tgcatcttgt ccgggttgat
      421 aagatttctg cgtagtatcg tgaatggtag cggttagatt ttccttgggc agttcctttt
      481 tgcttgaatt gcttttacaa gccgaaatta caactaaggc aatcaagaag atagataaaa
      541 agtgtttcat atctggaaag tttaaagaag gcaaaattat caatctttac agtaattcat
      601 caagtatttt attgcaggtt catatccaag ttctatggcc attttcaagt cggcacagcc
      661 agtatccatc tgatgctggg caaggcggct aagacctctt cctgtgagag cttcagcgga
      721 tgaatctatg gaataggcca tgttgtaatc cgattcggct tctgcaaatt tgtaaagatt
      781 aaaactagcc atagcgcggt taaaatatgc ttcggcataa ttgggattta aataaataac
      841 tcgggtaaaa tcttcgattg cagtgtagta ataaccagct tcggctttcg cgacgccacg
      901 gctcaggata atttctgcat cgtttggcaa aagcgccaag gcggcatcga agtcggcaat
      961 ggcctcgaag aaccttccca gatgatgttt gcataaaccc ctatgataca aagcttgagt
     1021 attttttgaa tcagatttga ctaatttatc gaagttcatg aatgcttgct cataattttg
     1081 atgttcgaaa tcgatgaggc ctaaattaaa aagggcgtcg gtcatggtag tgtcaagtga
     1141 aatagtcttt cgaaagtctt ccctggccag ggaatcattt cccagagcgt gataaatcaa
     1201 accacgttga tagaatacat tggtttgctt tgagttgatt ctggctgcct gatccatatc
     1261 gaaaagggct ttctggaagt ttcctaactt gtaatagagc acacctctat tataccatgc
     1321 ctcgggcagt gtaggatctt tatagatggc ggtgttgaaa ttctggaggg cctgttctgt
     1381 atatcccaga cgggctgctg ttattccctg ttttatgaaa tattctgcat cattctgggc
     1441 tgaagattca ataatgagat tccaagaaat aaaaagcaac aagataattt tgaaatattg
     1501 tttcatgatt gtgaagcaat tactcgtacg atttcattag cagctctttt cgaagccccc
     1561 gggcctccca acaaatgcct aatttcgtcg tatcctttgc gaatctcgtt tctatatcgt
     1621 tcatctttca ctatcttgtc tatttcgtcg tataaacgtg caggagtaaa atagttctgt
     1681 atgagttctt ttactacttg tcggccggcg ataagattag gtaagccgat aaatccgatt
     1741 tttaccatac gtctggcaat aaaataagaa aggggctgac ctttataaca aattacctga
     1801 ggcacattca gtaaggcggt ttcgagtgaa gcagttcccg aggtaactaa agctgcataa
     1861 gaatgactca gcaaatcata ggtttgtccc ttagtgattg caattctatg atgattggta
     1921 attggttcga aaaaattgtc gggaagagaa ggagctgctc caatcacaaa ttggaattca
     1981 gggaaacgct ttaccatgct gagcatcacg ggtaacatca cttttatttc ctgttttcga
     2041 ctgcccggca aaattgctat tataggctgg tgattcaaag aatttcgact gaggaattct
     2101 tctcgagagg gaggaatata ttcggaaata acatcgagca agggatgccc tgcataaaat
     2161 acctcaaaat ggtgttgaga ataaaaatca ctttcgaaag gaagtatagt aatcatgcgg
     2221 ttgacgtatt tctttatgct acgtacccgc gattgatgcc atgcccaaac ctgtggtgat
     2281 acataataga tgactttgat gttatttttt cgggcaaatt tggctatcct caaattaaaa
     2341 cccggataat cgacaagaat aagagcgtga ggttgaaagt ttaagatatc ggatttgcat
     2401 tcatttagaa tttttataat ggtagggaga tgaccggcca cttcgacaaa acccataaat
     2461 gagaggtctc tgtggtgttt tatgatgttt accccagctt tcttcatcaa atctccacca
     2521 aagccacgga attcggcttc gctgtcgagt tcttggattg cctttaccag attggaagcg
     2581 tgtaaatcac ccgaggcttc acctacaatg agatagtatt tcatatagtt cccgccttta
     2641 tccttacata aataaaatag cccaatacaa ggacaaagac catggttaaa actcctctac
     2701 ctgtgcgttc gaattttcgt cgcataaaaa aatgtcggat caaaacgatt gggatgataa
     2761 gtgaaagcca atacatttgc catggttgaa aaaatggatc taatcccgta attgcagtaa
     2821 gaagtaagcc gatgagccag aacagtattc ctccggttat cattccgagc agtccggcta
     2881 aaagtccaaa ccaataggta ttcttattca ttgttgaata ttttcttcaa gtaatccaag
     2941 gcataatgtg ccgtcatgtc gtactgaaca ggcattaccg aaatgaagtt gttttccaag
     3001 gcgcaaagat cactacccgg ttgcccatcg taatttataa aattccccat tatccagaaa
     3061 taatctcttt cgcgtggatc gaggcgatga tcgaattctt cttcccatct tccttcaccc
     3121 tgatgtgtaa tatgaattcc acggattggt tcacctcctg tttcgggaat gtttacattc
     3181 aagcagactc ctcttggtaa tccattttcg agaataatgc gtgcaattct ggtaacccat
     3241 tcatcgaggc aactgaaatt ggcatggtat gaataatcac aaaacgaaaa acctatgctg
     3301 ggaatatcta acatgcaacc ttccaataca gctgccatag ttccagaata cagaatgttg
     3361 atagaggcat tagaaccatg attgatgccc gaaaccaaaa gatcaggttt ccggggtacg
     3421 agaaattttt cggccagttt cacacaatcg acaggtgtgc cacttatacc gtattcctca
     3481 taattttctt cctcgctaat tttctgataa cgcaaaggaa tatttacggt gatggaatga
     3541 gccatccccg actgaggttt atcaggagcg gctacaataa catgacccat gggccgcatc
     3601 attcgtatca aggctttgat gcctggagca ttcactccat catcattggt aactaaaatg
     3661 agtttattat ccataaacat tcctcttcaa aaataaaata atctccactt aaaataactt
     3721 taccaaattt tttttcattt aaaaatgaaa ctaatgtcac catcattata aaaaagaaac
     3781 atattaaata acacttcctt cggtaatttc atttttatga attaagtaat tagaaatatc
     3841 gaggttcatc cttttgaatt gacaaagtta acagtattct atgataagga ttaaaattaa
     3901 gttaaacaat cagaaggttt gaagatgctt gtataggatt tgagtaggca agcccatcac
     3961 gttgtaaaac gaaccatgaa tagcttctat gccaatgtat ccgatccatt cctgaacgcc
     4021 atacgaaccg gccttatcat atggagaata atagtcgaca taatattcga tttcttcttt
     4081 ttcaagtttt ctaaatctta cctcactttc ggccacgaat gtttcggttt ttcttgatga
     4141 acgcaagcaa attcccgtgt atacgaaatg agtattgtta gagagttgtt tgagcatgat
     4201 gatggcctct tgtctcgaag agggtttgtt gaggatgtga ttattcagcc aaacaattgt
     4261 atcggccgta atcagaagaa agttttcggg caggtcttca ttattgaaag ccaaactttt
     4321 gcgtcgagca agcatcaaag cgacatcttt cccgtgcata tcgagagggt agtcttctgg
     4381 agtttctttt gtaaagacca ggaagggaag gtcaagcccc gcaagcaatt gctgccttct
     4441 gggtgagcgc gaagctaaaa taagaatgta atcttgtagg ttggatagca tttcgaaatt
     4501 ttacctggat tgtttcaata tgattgaata tcaaaataat ggttgatcat ttctggtgaa
     4561 aaacctcata aatctctcct attggctatt cgaggtattt tccaacaata ggcattctgc
     4621 gtccactacc gaacgccttc ttcgataccc tgaggacagg aggcgcttga tatcgtttat
     4681 actcgttgga atttaccatt tttagaattc gaagcaccaa tgctgaatca tgaccctgag
     4741 cgatgatttc tgtcggtcct tgctgttcct ctatatagag acgaagaatg ggatccagaa
     4801 tttcgtataa gggcaatgag tcactgtctt tctggttagg gcgcaattct gccgaaggtg
     4861 gttttaaaag ggtactctcg gggatgatag ggcttttgca attgatataa tgagcaagag
     4921 cataaacatc tgttttatag acatcgccca aaaccgataa tcctccgcac atatcgccat
     4981 aaagtgtacc ataacccacg gcaagttcgc ttttatttga agtattgagc aaaatatggc
     5041 caaatttatt cgaaaaagcc atcaggataa ttcctctaag gcgcgcctga agattttctt
     5101 cggtgacatc ggaaggcatt ttcttgaaaa aatattcaaa tgtgttttct aaacttttat
     5161 acgccttatc gatgcttatt atttcaaaat caacctctaa tcgttgagat aatagaatag
     5221 cgtcagtaat actttgaggt gcggtatagt gagagggtag aatgagagcc tttacatgat
     5281 cattcccaag tgcttccttt gctaaaacca gcaccaatgc cgaatctatt ccgcccgaaa
     5341 gtcctagaat ggcatttttc aggtttaatt tgccaaaata ttcctgtatg ccaaaaacca
     5401 gtgcatcatg tatcatttct atacgtgaaa ggggataatg aggcagggca ttggatatcg
     5461 aatggagttt tttggtatcg acaatcacca tgtcttcggc aaaaagtttc atgcgtgcta
     5521 cgatatctcc ataaatgtca ataaccatcg aatctccgtc gaatatcaaa tcggtatgtg
     5581 cgcccacctg attgacgtaa ataagaggta tttgggatct tacggtgttt tctttcagta
     5641 ttttcggcct cagctccgtt tgattgtcgt gaaatggtga agccgaaata ttcaccataa
     5701 gcgatggatt taatttaagt agttcatcct gtggtttgca taaataaagt gggttattgt
     5761 cggtatcaag taaatcctcg cagatgcaaa gcgcaattct ttcacctcta aattcaaaaa
     5821 catcgaactg ctggttgggt tcaaagtagc gatattcatc gaatatatca tcatcaggga
     5881 gcaaggtttt gcgttgaatg ccaagaactt gtccttcata taataaaagt gcagcattaa
     5941 acaagggttt ccctttcctt gtttcgtttt ttatgggact tcccacaatg gcagcaatat
     6001 ctcgacaggc tcgactaatt ttctgaaccg attcttcgca tttttctata aaatcatcga
     6061 attctaataa atcttttggg ggatacccac atactgagag ttcggaaaaa atagccagcg
     6121 aagctccttt tgattttgct tcctgtatag attggatgat tttttttgtg tttccttcga
     6181 agtcaccaat gatgtaattg agttgaatca gagctatctt cataagatcc gatctaacgc
     6241 tttgattatt agaaaaataa agccaaagaa agttccaaat aattggcaat cccatcttcc
     6301 gaagcattta ctgaaggatg ggagttataa tcggaaaaga tatccgtaaa tgaattattg
     6361 aaacccaatc caatcatgat aaaggtggat tgatcgaagt ggtattcgaa tccacctcca
     6421 acaaaaatgg agagtttcat agcatttaga gccgatgaaa catcgggttc gtttttcaag
     6481 gtcgttccct gttgggaata aaaaatatcg ttagcatggg ctcttagttt aaaagcaggc
     6541 actaaaccca tatctccata aaatcgagtg ttttttttgg gtagtgtttt catcttgaat
     6601 agtaaaggaa tttcaagata acgatagcta taattgcggt agacctttcc gagaatcaat
     6661 gtagtatcac ctattccatt agtcttaggt ttgggtattg gatagtttac cgggtagcaa
     6721 attttacctc cttgataact taatccaaac ccgctggaaa agaaataatt atctcgaaga
     6781 ttgatgtcag caattaaacc ccacgtgaat ccaatttttc caccatcgtt tttatagttt
     6841 cgcgtttcag gatttatcca ggtgtaattt ggactgactt ttattccaaa ttgatacttc
     6901 ttttcgcttt gttggccaaa aatattcaga gccgcaacca tcacaagcaa taacccgata
     6961 aactttttca tggtagtttg aattttaaac aaagatacag gcatttattg atttataaag
     7021 gttgaaataa aagtattgtt ttgttccctg taatgtaggt ggtgaataag atgcctgtga
     7081 cccggtacga aggtgaaatt caagataaca aatgacaatt gctgatgaaa cattgagggt
     7141 aaataagccc aacgatattg gtgtttttgt ggttccgaca ttttttttct ggaatttcac
     7201 ggcttggcga agagaaagtt cccccaaata cataaaaaaa gctcgccgtt ctttatttcc
     7261 acatcctgta tttccattat ctttatataa attttatggc catttgatat tttatataat
     7321 tcagtgaatt tcaaaacgat aatctttctt tcggctttat tgctggttaa cttttacggc
     7381 atgacacaag tgagggtgaa ggtctggtcg gatgaattta attatagcgg cctgcccgat
     7441 actaatcttt ggggatatga tgttggagct ggtggatggg ggaacaacga atctcaatat
     7501 tacacggatc atcgttggtt aaatgcaagg gccgaaagtg gtaagctgat tattgaagca
     7561 agaaaagaaa attttgaaga ctgtgaatat acctcggcaa ggcttgttac gcgcaacaag
     7621 ggagattgga aatatggtcg catcgaggta tctgctcgat taccgcatgg tcgaggcaca
     7681 tggccagcca tctggatgtt gcctaccgat tgggtttatg gcagttgggt taaaagtggt
     7741 gaaatcgaca tcatggaata cgttggttcc gatcctggta tagttcatgg cactattcat
     7801 acacaagcct ataatcatat tattggcaca caaaagacgg atacatttta tgttcctgat
     7861 gccgaaacca acttccatct ctatgccatt gaatggacag aagaaaaaat agatttcttc
     7921 gtagatgatg aaaaatattt cagtttctat aacgaacata cgggttacac tacttggccc
     7981 ttcgatcagc gctttcattt attattgaat attgccgttg gcggaagctg gggaggacaa
     8041 caaggaatag acccagatat ctggccacaa aaaatgtggg tagattatgt aagagttttt
     8101 cagaatattc cttttgagga aattcatatt gaaggtccat gctgccttca ggccggacaa
     8161 gctaatgctg tttttgaaat tcaagatata gaaggagcta tatttcattg gactgttcct
     8221 gaaggagcta ctatagaatc gggacagggg actcataaaa tcaaggtaac atggggaaat
     8281 gatgaagaaa atgtaaaatg tgaagtcatt catcctcatg ctggcggaat ttattcgaag
     8341 gaggtattca ttgctcttgc tcctgtttat tcgaggtatg atctcaccaa ttttgccatt
     8401 caggatactg aaggatggac aattaccgct aatccaccta atcaagtcaa tatcttcaag
     8461 caagatacct tgatgcaact caattattca atatttccaa atgcgggtaa accttatctg
     8521 gaatatactt ttcctacaat tgtagatatg aaccagcatc ctcatctgaa cgtgtttctg
     8581 aagactttta ataaaagtgg tactgcagcg acaaagatcg aacttttcga ttctcgaaag
     8641 cgattcaccg atgttgatcc cggattttcg attctgcctt tgcaactttc aggcgaaatt
     8701 caagccttcc atttcgattt tactaaccat tggaacacca atctaccctt tccaaattcc
     8761 aaggtagatt ccaactgtat ttcaggcttg agaatatact tggatacacc tggttccgaa
     8821 tgtccctgct tcgattcgct ctggatatcg agaatcgaaa tcagcaagga aaattatttg
     8881 ggaatcagca accgacaatc tgagcccttc attcgttttt atcccaatcc ttttcgacaa
     8941 actctttaca taaaatcgag taaaaacttt cccgatcatt tttatatctt tgacctcaca
     9001 ggtagagaag tttttcatac aattatcaat caacccgtca accttctctc tctcgatttt
     9061 ctcaacccgg gtgtttattt gggtgttttg aaaaattcaa gatatcgcca gtatattatc
     9121 aaacaataat ttattgctcc ttatagactt atagactcat tattgtttat caaccaacaa
     9181 tatggttttc tggtaaataa tattacagct attctttatt aaaataatca aaaccaaata
     9241 cttagataga gatttatctg tgactttctt aaaatgaact tttaaacgag taagctttga
     9301 aatagatttc acgattgaaa aggaggataa tttactcgaa ggacctgcta agccagaatc
     9361 ataaattgat taatttttaa tcaatatttt ttaatcatct caatgaaatt ttggtgaaat
     9421 gatttcaaaa ttgagcaaaa taagaaaatc tgtattaatt tagatgagct caatttgatg
     9481 taaaattaat caaaaaatat gctgaggatt gcgcattctt actatagtct gagatatgga
     9541 actctttccg tagaagattt ggtcgaatta ggtgccgagg cagggttaaa gcgtctagtg
     9601 ctggccgata tcaataacac ggtaggtttg cccgattttg taaaagcctg tcatcgagcg
     9661 gacatattac ccatagccgg tatggatatc tttaaggaga atcatcggct ttacaccttg
     9721 atagcacgga atgcaaatgg ttttcgggag atgaatgaat ttataagcca gtacaacagc
     9781 cagcaaattc cgtatcctga cgaagctccc gagatgaagg aagttattgt gatttatcct
     9841 tttagcaagg catttacccg acctttcagc cctcatgaat ttattggcat acgaccaagc
     9901 gaactgaacc gcctgatatt ttcacctctg cgcaaccaag tcgatcacct gcttgccatt
     9961 caatacatca cttttgccaa tgatgaggga tatgagattc atcgaaattt cagagccatc
    10021 gacaataaca ttttacttag caagctaagt tcagatttat tggcggcaaa agacgagagg
    10081 ttgctcgtgc ctgaggcgag ccagaacttt ttgagagatc acgaagcgct ggtgcaacat
    10141 accgaaagag tctcagaaag ttggcagata gattttgatt ttatgacttc gaagaataaa
    10201 aaaaccttca cggggaacag ggaagacgac aaggttttgt tggaaaaact tgcgctggaa
    10261 ggaatggttt atcgctacgg aaaggggaat gccgaagccg agcagcgagt gcgatcggaa
    10321 ctcgaaatta ttcatcgttt aggtttttcg gcttatttcc tcattgcttg ggatataata
    10381 cgttacagca tgtcgttagg gttttatcat gtagggcgtg gcagtggggc caacagcgtc
    10441 gtagcttatt gcctgcgcat caccgacgtg gatcctatag agctgaatct ttattttgag
    10501 cgttttatca atcccaaacg gagcagtcca cccgatttcg acatagatta ttcgtggaag
    10561 gacagagata cggtgattga ctatatcttc aaacgatatg gtcgtcagca tacggctttg
    10621 ttggggagta tgtccacttt tcgcgatcgc tccatctatc gggaattagg gaaagtttat
    10681 gggcttccca aacatgaaat cgatgagctg gccgaacatc cggaattcag aattcaacgc
    10741 gaccatattg cccgacacat acatgagatt gcctgcaaaa tagcggattt ccccaatatc
    10801 cgctccattc atgcaggagg tattcttatt tcggaagaac ctattactgc ctataccgcg
    10861 cttgatttgc cccccaaggg atttttaaca acgcagtggg atatgtatgt agccgaaagc
    10921 cttggattcg agaagcttga cattctgagc caaagaggta taggacacat taaagagtgt
    10981 gccgatattg tacttcaaaa ccgcggtctc aaaatcgatg ttcatcaagt cgatcaattc
    11041 aaggccgacc ctttggttcg cgaaaaaata cgtaatggag aaactatggg atgtttttac
    11101 atagaaagtc cagctatgcg aggtttgctc aagaaacttg cctgcgacaa ttacttgagc
    11161 cttgtagcag ccagcagcat cattcgtccg ggggttgcac gttcaggcat gatgcgtgaa
    11221 tatatctacc gttttcatca tcccaacgag ttctcgtata ttcatcccat catgaaagaa
    11281 caattggccg agacctatgg cgtgatggtt tatcaagaag atgtattgaa ggtttgccat
    11341 cattttgccg ggctcgacct tgccgatgcc gatgtactgc gcagggccat gagcggaaag
    11401 ttccgcggga aaaaggaaat ggcacgcata gtagatagat tttttagcaa ctgtcgtgaa
    11461 cgtggatacc ccgaggatat catcgcagaa gtttggcggc aaatagcatc ctttgccggt
    11521 tattctttct cgaaggctca ttcggcatcg tatgcggtcg aaagttacca aagcctctat
    11581 ctgaaagccc atttcccctt agaatttatg gtagccgtca tcaacaactt tggcggattc
    11641 tattccacct gggtttattt taatgaagct cgtcgctatg gtgctcgaat tgaactgccc
    11701 tgcgtaaaca agagtcgagt ggttacctcg atagagggaa acaccattta cattggattc
    11761 atccatatcg aaaacctcga acgtgatttt tcgcaactta tcgtaagcga aagagaaaga
    11821 aatggccttt atcttgacct ggccgatttt atcgaacgca ctcaggccaa actggagcaa
    11881 acccttctgc taatccgcac aggagctctt agatttaccg gaaaaacgaa agccaatctg
    11941 atgtgggaag cacatatctt cctgaataaa aaaaattctc aggcttcttc tcaacctccc
    12001 ttattcaaaa tttctgcacg acctttccag cttcctgctc ttccttccaa ttcgcttgaa
    12061 gatgcctacg acgaaataga acttcttggt ttccccgtta ccgtgtctta tttcgatatg
    12121 ctacaaaccc gttttcgtgg agaagtaata agccgaaaca tgatacagca tgtcggcaaa
    12181 accgtaagaa tgctcggata tttagttaca ctcaaatatg tacgtaccgt taaaaacgaa
    12241 ttgatgcatt ttggaacctt tgtagatgcc gaaggtgaac tcttcgaaac cgtccacttc
    12301 cccaactcgc tcaagcaata tccctttcat ggaaaggcca tgtatctgct cgcaggaacc
    12361 attaccgaag aatttcaaca tccctcgctc accattacta aaatggcaaa attgcctttt
    12421 cagcccgatc ctcgacataa aaattaaact atcgaaggaa acccgggttc gcttcagcat
    12481 ccgcctgggt cgaaccaccc tggcaagctg tatcttcatg atttttcgca gctactttct
    12541 tcgcttaaca atagaactat gtgttataac taaaacctga ccttcccaac atttcatatc
    12601 tcttgacggt gtttcaataa tatcattagg tcatctacct gccactatcg aatcccctaa
    12661 caggattaat tatacataaa attttcgcac aactaactga ttgaggaaga atcagtttat
    12721 ttgtcatcta aggacattat agaataaact caacaaattt aagtggaata caatctttta
    12781 gaattaattt gtttttaatg cgatttgcaa ctgctgacat tttgtggcta aatccagact
    12841 ctgagctaaa cttatagaga ataaatctaa tctaatactt ccttgaatcc ttatttggct
    12901 aaattctcag atttacgagc cattgaagcc caaactccga gaatgcccaa aataacgaag
    12961 atgataaatg ttgttcgttg ggaatggagg aataatgaca aattgcctgc atgaatcttc
    13021 gaattgccga ggaagatatt tattactaaa gttgcaatcc ccatgctgat catttgtccg
    13081 gtcaaacgca tagtacctac agtagccgag gccaccccaa ggtatttctt ttctaccgac
    13141 cccataatgg catttgtgtt gggtgaggta aacaaaccaa agcctattcc aatcacccca
    13201 agaatcacca ttaaataaat gagggatgaa tttgttccca aggggatgag cgccataaga
    13261 ccgagtgtga tggtactcat gcctgcagag gccaaaatac gagggtcata tacatccgac
    13321 atacggcccg aaaaatacga cactatcacc atcattgcag gctgaatcac cagaatcaaa
    13381 cccgcatcgc ggggattgag gccttttaca tactgaagat ataagctcaa cataaaagta
    13441 atggcaaaag taatggcgta attgagtaaa gctgccagat tcgacatggc aaaaagccgg
    13501 ttatggctca gcaatcgcat atcgaatacg ggaaaatcgg attgaatttc tatttttacg
    13561 aaaaaatata aaccaattaa agaaatgaaa gccaatatga tagcccagaa attcgggaga
    13621 tggctaaaac catacataaa tgccgacatg gcgatgatat atagaattcc tcctttccaa
    13681 tcaaattgtt ccgaatgtgg gcttttccat tccgttttta cgagaagagg catggaaata
    13741 gccacgaaag ccactggaat agcagttaaa tagaatatgc tttgccagcc ccagttttta
    13801 acgaggaaac cggcaataag aggaccaagc gacagtccca aataaaccgc cattacgtta
    13861 gtaccaatgg ctttgcctct ttcgttggca gggaaaatat ccgtaataat ggccatattc
    13921 gttccaaaca tcattgcacc cccagcaccc tgaagcacac gagaagccaa taaaagcccc
    13981 acactcggac tcatggcaca aaaaatagaa ctcaatgaga acaagatggt cccactaagg
    14041 aaaactctct ttcgtccaat gatatcggct attttcccca aaggaaccat taaaacagcc
    14101 gatgacaata gaaacgacat ggctacccaa ctcatcatca cagcatggag cccgaagtgg
    14161 gaaccaattg caggtaaagc aagattaatg gatgatccca taaaaggatt tgcaaacgag
    14221 gtaatcagta taagcgtaag aatcaaattg cgatgccgag tcggaagttc tttctcactc
    14281 atgacaaagg ttttgataaa attacaagct ttgagccaat ggtttacttt atcttatagt
    14341 ctttaccttt aattttattc gtaatgcatt atttatcaat tcataacaaa aatacaaaga
    14401 agagcatttt tatattatca tagtgcaaat cgatacctgt tatcactatt gtgattaggc
    14461 tgaaaaatca aagttcgaaa tataaagagg cgccgatgta aatttgcaaa aatgatgcat
    14521 aactgaatga attcaaaaac ttcatcgaaa atatacgttg ccatgcctgt tatggcagaa
    14581 tatgaatatc tgcctacttt gctcgaatgt ttggagaatc aaagtttcca gaatttcaag
    14641 cttgtagttt gtgtaaatca acccgatgat tatcgcttca atcccaataa aatccacatt
    14701 tatgaagaca atcagaaatg cttgagttta ctgaaatcca gttcatattc atttgatatc
    14761 gagattatcg ataggtcttc taccgatatg gggtggaaag ggaagcattg tggggtaggg
    14821 caagcccgca aaacagtgat ggatgctatt gcacgggaag ctaaaaataa cgatgatatc
    14881 attgtgagtc tggatgctga tacgctattt gggacagatt atttagaggt tatagcccag
    14941 acttttcttg attattctta tattgctgct ttatcggttc cttattatca tccgctcacc
    15001 caagatgaaa ttaccaatcg ctgtattctc cgttatgaaa tatatatgcg ggtgtatgct
    15061 ctgaatatgt tattcattca taatccctat gcttttacgg caattggttc ggctatggct
    15121 ttaagagtcg atatttaccg aaaagtagga ggcattacac cgcatcgagc tggtgaagat
    15181 ttctattttt tacaaaaact ccgtaaacat ggttcagtgc ttacgtggtg tccagcgaaa
    15241 atctatcctg ctgcacggtt ttccgataga gttatctttg gcactggccc ggcaatgatt
    15301 cgaggcaggg aaggccattg ggaggcctat cccatttttc accattcatt gttcgatacg
    15361 gtgaaaatta ccttcgaggc attctcgaga ttatacttag aaaaaataaa aacgcctatg
    15421 gatgtttttt tagaagatat ttttcgaact tccgacattt ggggaccact gcgtaaaaat
    15481 tccagtgaga taaaatcgtt tgtaaaggct tgtacacaga aaattgatgc cttgcgcata
    15541 cttcaatttc tgaaaaaata ccatcctcaa atcaaaaaat ctgacgagat ttgcctcaaa
    15601 gaattcatga tgcattattt ccctgagagt catccagcgc tgatcgaaag tttcgacact
    15661 tgcaatataa atgccttgca ggaaattcgt gattttctta ctatgaaaga atattcttat
    15721 cgacagcatc atcctttgct tcagtagttg cttttataca cttttataca tgattacaat
    15781 acttggacct acggcagttg ggaaaacacg catttcggct atgatagctg ctcggatgga
    15841 tgccgagatt atttcggccg attcacgtca agtgtttcgt ggaatggatt taggcacagg
    15901 caaggattta caggattatt tcgtagatgg cagttttatt ccctatcatt tgatcgacat
    15961 ccgtcagcca ggcgaagaat atagtgtatg ggatttttca tgtgatttca agaaagctta
    16021 tctcgaaatt cgtagtagag gcaggcaggt aattctatgc ggtggtacag gtttatatat
    16081 cgaagcagtg attggagggt attatatgcc tcaggtaccc gaggataaaa atttaagaaa
    16141 ttctctcgaa aaagaatcta tcgaaaatct tatcactcgg cttgcatccc tgcgaccttt
    16201 acacaatact accgatatta ccgaccgagc tcgtttaatt cgtgccatcg aaatagaaac
    16261 ttggaaactt tcacatcccc gagaagaaac cttcgtacct cccaatttta ttcttggcat
    16321 tacattaccc cgagaagagc ttcgaatgag gattacccaa agacttcaca aacgaataga
    16381 gcaaggcatg atagaagaag tagagcagct caagaagctt ggcataagtc acgaaaatct
    16441 cgattcctat ggattggaat atcgctttat aagccgttac ctgaaagggc agatgtcgaa
    16501 aaatgaaatg gtagaatcgt taaatacggc tattcatcag tttgcgaaac ggcaaatgac
    16561 gtggttcaga aggatggaac gcaagggatt tcggattcac tggttagatg gaatgcaatt
    16621 agctgaagaa gtcgtgaaaa aggctatgca ggttctcgat agccatccag atcaatggaa
    16681 ggaatgatta aaatgacaaa gaatatgaac gaaagaaaaa atattatcat tacaggagct
    16741 acgggtttta ttggaagttt tcttaccgaa attggcgtcg aaaaaggatg gcaggtaacc
    16801 gcctgggtaa gggaaacaag taataccgaa catctccctt ctggagtaaa tctcataaaa
    16861 gcgggttttt ctaatcgaca gaagatgatg gaagcattac agatgatcga gcgccagaat
    16921 ggttccatcg attatatcgt tcataatgct ggagttacaa aaacaactca tcctgaggac
    16981 tttgataggg tgaatgcaga aaataccggc agatttatcg aagctattca ggaaagtggt
    17041 atttcaattg caaaatttat tctgatgagt agtttggcag ccttggggcc aggcaatccc
    17101 caaaccctcg aaccacttca tcctgatgat gttccaaaac ccgatacctt gtatggaaaa
    17161 agcaagttaa aggctgaaaa aattgttcgc caatcgtctt taccctggaa tatcatgcga
    17221 cccacaggag tgtatggccc acgagaaacc gattattacc tcatgctccg cacaataaag
    17281 aaaggactgg ctccagttac tggctgcaag cctcatttcc tttcttttgt ctatgtaaaa
    17341 gacttggtaa aagccgtttt tttagctctc gaaacaccta ttacacaaca aacatttcat
    17401 gtttctgacg gtaaaaccta taccgatcgt gaatataggg atttatgctg caaaattctc
    17461 aatacaaacg ccttgaaggt aacggtaccg cattgggttt taaaagccgt tagttttatt
    17521 tcggaaacct ttgcccgacc ttttaagatt acacctaccc tcaataagga taaatattta
    17581 actatgagag caataaactg gaaatccgat atcagcaata ccatcgaaat gcttcattat
    17641 tctcctgaat acgatctgca acgtggtttg gacgaatcta tccaatggta tagaaaagag
    17701 ggttggctgt aaaatggggg atggggaatg gtgaattata agttaaaata atgaaaatga
    17761 atatgttagt attaaaaaat actccctgaa atccccctcg ctcattctcc ccgtctcttc
    17821 gtctcctcgt cccgtcgtcc cgcaatctct tcataatctt aaaggcatcc tcgcattacg
    17881 tattgcatat caattttcac ttttcatctt ggatgtcgta cttttgtttc actttatctt
    17941 ttttcatgct tacatcttat gaatctctat tcgcgtaaga gaaaatggaa atggttattg
    18001 ggtttatttg ctctgttgat cttcgggagt acaatttttt atacggatca tcttgtaagg
    18061 caaattaaag aagccgacca gaagaatctt cgtatatggg ctgatgctgt gaatcgcaaa
    18121 gctgcattag ttaattatac cgataatttt ttccgacaaa ttcaggaaga agaacgccga
    18181 cgggttgaac ttttagcaga agcttacaaa cgattgatag taaccgacga tcaggccgat
    18241 ctgacctttt acctcaacat gctcgaaaag aataaaacta tccctgtgct tcttaccgat
    18301 gaaaactata acatactgag tgctactaac atagatatcg acctatcaac tacaaaaaaa
    18361 ttagaaggag ccttgctgca ggaatttaca aaataccctc ccatcgaagt cccctatatg
    18421 aaagggaaaa aaaattttct cttttatacc gacagtcgat tatttagtga actgcatgaa
    18481 gtcctcaacg acctcaacca gtcctttatt tccgaagtag tacttaattc cgcttcagta
    18541 ccggccttga tcatcgacag taccaaaagt caaattcttg ctttcggaaa tatcgatagt
    18601 atattgcttg ccgattcagc ttcgcgcaat aaaattatcc aggaactttc ggtagccaat
    18661 gtacccattc ccatcacact ttccgaacat gggaaatgtc tggtgattta tcgtaattcg
    18721 ttcctgatga atcagttgaa atattatcct tttattcaac tgggcattat aggtttgttt
    18781 ctgctggtag cctattctct gttcagttct gctcgcaagt cggaagaaga ccaggtatgg
    18841 gtagggatgg ccaaagaaac tgctcatcaa ttaggaacac cgatgtcgtc gatgctagca
    18901 tggatggaac tcatgcggat ggatggattg gaacatcccg ccatcagtga gatggagaaa
    18961 gacttaatcc gcttggagac tattacggaa agattttcta aaatagggtc acagccttat
    19021 ctggaggctg ccaatattct cgaagttatt tccgaaactt tcgattatct cgaaccacgt
    19081 acttcgagaa aaattcaatt tcgagtcaat aatcaaacca ataaggatga gttgtatata
    19141 cctttaaatg ttcctctttt tgaatgggtt atagaaaatc tggttaaaaa tgccattgat
    19201 gctatgggag gagtgggtac tttcaccatt acgatacagg aggatcataa acatgtaatt
    19261 cttgatttta ccgatacagg gaaaggaatt ccacgttctc agcaacgagt gattttcaaa
    19321 cccggattca ccaataagaa gagagggtgg gggctaggcc tttcgcttgc ccgtcgtatt
    19381 gtacattcct accataaggg aaggattttt gtaaaatcgt cccagcctaa tgttgggacc
    19441 actttcagag tgatccttaa taagaagaaa tgactggcct ctatattcat atccctttct
    19501 gcaaatcgaa atgcctctat tgtaattttt atgctgtggc ttctaaaaaa ttagctcagg
    19561 gttttgtaga agctttgcta caggagatag aaacccgttc tcaccagtgg aaagatcctc
    19621 atctgcgaac cgtttatatg ggaggaggta ccccttcatt gctccttcct ggtgatattg
    19681 aaaatatttt agatttcata aagcgatatt ttattctcga gccttcctgt gaaattaccc
    19741 tcgaagccaa tcccgatgac atcagtatgc aaaatcttga atcctggtcg aaggctggaa
    19801 taaataggct gagcatcggc attcaatctc aaaatgaaac tattttaaaa tttttaggcc
    19861 gacatcacac tgccgaacaa gctttggaag cttatgcgat ggccagaaaa gcgggtttta
    19921 acaatatttc ggtcgatctt atttatggta tccagggttt gaataatgaa gaatgggaaa
    19981 atgctcttct taaaatctta tcacttgaac ctgaacattt ttcttgctat gctcttacac
    20041 ttgaagaaaa aactccttat gattttcagg tgaaaaaagg gaagctaagt ctgcccgacg
    20101 aagaacttgt tgcccagcaa tatgagattt tacaagaaat ttcctcgcat aagggttaca
    20161 ttcagtatga aatttcgaat ttctgtttgc cgggatatta ttcgaggcac aatactgcct
    20221 actggcaggg aattccatat ttgggactag gcccatctgc ccattcgtac gatggcatca
    20281 aacgatggtg gaacccttca tgtatagagg aatggaagaa attgactact ctcgacatcg
    20341 gtgcatgtcc ctgtgagatc ctaacatcta tcaaccagta caatgagtac atcataactt
    20401 ctctccgaac ctcttggggc ttgagcattt ctcacatcaa caaacgtttt ggtgaaaaat
    20461 tcacccgaca cacccttcaa attgcgaatt ctttaacaag tatctattta aagaaaattg
    20521 gtactgacag aatcgcaatt aagtcacagg cctatattct ggccgattgt atcctcgaaa
    20581 gatttattct catggatgat tctaagatat aaactccaga ccaagagatt aataatttta
    20641 tattttgatt ttattgaggt ttgattcatt cccttaactt tgcacgctat tttctataaa
    20701 tgtttttatt gatttttacc ttcttttaaa tcaatcagtt atgcatattt atcgttttcg
    20761 gattacttct gattccatcg aagattttgt aagagagatc gatatttcgc ccaatcagac
    20821 tttcgaagat tttcaaaggg tttttctcca agctgctcct ttatcggcag aagagaaaac
    20881 atatttctat aaagccaatg cacgttgggt aaaacaatat gagatagcag aacaacccct
    20941 gttatccatt tcgaaagaaa aagaaggcga tgatgttaga ctcgaagagc atcgtttgaa
    21001 aattccctat acgcatatca gtcttgcacg ttttagggat tttgtggatg accctcatca
    21061 aaagtttatc atcgaatatc gaggtcctga tatttatttg ttttttatgg aaatggtaaa
    21121 aatactggaa accgattctt tagcaaacta tccaaagtgt gtaacacaaa aaggagaaat
    21181 acccgtaaaa cccatcatca tacctgaaga aattcaggaa aagacacgta aacaacgaga
    21241 agaaaggctt accattactt ctttgaatga tgatattccg gaagaaatga tagaggaaca
    21301 gatgaaagaa ttaatggaag atgaggtttt tggtgcgata atgagtggga aggaagtccc
    21361 tccatcttcc aatatttcac gcaagatgac agccagagaa attgccgaag aatttggacc
    21421 cgagtttgcc gatatgatgt cgactgatgc agatgaaaaa ttcgatgacg aagatattac
    21481 cgatgaagac atagatgacg aatttgccga cgataaatcg ggcgaagact tcgatgacga
    21541 tttctaatgt gtcttcaatt ttctctacat ccaaatcatg aaacttcaga aacctttatt
    21601 ggttgtgatt gcagggccga ctgcttcggg gaaaactgag cttgcgatag aacttgcctg
    21661 taaatacaat acggagatta tttcagccga ttcacggcag ttttataaag aactccctat
    21721 aggttcggct acgccttctg aggaacaatt ggcaaaagtt aagcattatt ttattggcaa
    21781 ccggactatt catcatcctt gtaatgtagc tgaatatgaa gaagaagtta tagctctttt
    21841 gagtagatta tttacatccc agcctatggt aatcatggct ggaggttcgg gtttatacat
    21901 cgatgctgtt tgcaatggcc tcgatatttt gcctgaacct gacctattac tccgccatca
    21961 tttacatgct ttgtaccacg aacaggggat cgaagccctg agaacccaat tacgcctcct
    22021 cgataccgaa ttctatcatc aggttgactt gaaaaatccg atacgattga tgagagcgat
    22081 agaagtgtgt atgttatctg gccagcctta ttcgagcctt cgtactggaa aatcgaaatt
    22141 gcgtagtttt catatcctta agcttgcgct tgatgtaccc aagcctttat tgatcgaaag
    22201 aatcaatcat cgtgtcgatg aaatgatgca aagagggctg ctcgaagaag ctcagagatt
    22261 gttacccttc agaaatcttc agcctttgcg cagcctgggt tataaggaat tgtttgctta
    22321 tatggaagga caatttactc tggaaaaagc tatcgaaaaa ataaaaatca atactcgaaa
    22381 attcgctaaa agacaaatca cttggtttaa aaaagaccca ctctatcatt ggattcctcc
    22441 gaatgacctc cagcatatcg accaattgat cgaaaatgca ttgcaacaat cttgattttt
    22501 taccgaaaat aaattaaaaa tcagtttttt ttatttttaa atgtgatctc catattataa
    22561 gtcgaaaaat atattttttg tgatttcttt ttttatattt attcatatac tttcaatttg
    22621 aatcgattgt gaaaaaaata atcatcaatc ttttacgtca tttaaccttt tggctgttga
    22681 tctttgcttc tggaagaata ttttttctga tttattatca tggaattatt gctcattctc
    22741 acataaaatt tacggagata gccggcctgt tctttcatgc tttgcggctt gatgtggcca
    22801 cattttgcta tattgcttca ttattccttt ttttatatgt aattgaaagt ataactaaat
    22861 ggagggcaat taatcaaatt gtgaagtact tgaatttttt attgattgga atttattttt
    22921 tgattgttgc cggtgaaacg ggtttgtatg atgaatggca tacaaagctc aacatgaagg
    22981 cgcttgcgta tctgaagcat cctgcagaag tgatagcttc agcccaaaca ctgacgctcg
    23041 tgataatgat acttttctgg ctagtatcgg tttttactgc ttgctggcta tataataagt
    23101 ttatcttcca ggcttcaaca aagcaatcat ctccaaaggc atggaaggca gttataactt
    23161 acattgtgtt acaacccatt ctcattattg gttccagagg tggcattcag caaataccta
    23221 tcaatcagag tcaaagctat ttttcgaaac atgctatctt gaatcatgct gccgtaaacc
    23281 cagctttcaa tctttacatc agttggttcg aaaaccgtca gttcaaggat aggaatcctt
    23341 ttatttatat ggatcaggcc gaagccaggc acattgtaga tagcttattt gccaagccca
    23401 cgactaatgg gatggacata ttgacaatat ataaacccaa tatagtgatg ttgattctcg
    23461 agagctggtc agccgatctg atcgagagcc ttggcggaga acctggcata acgcccgaat
    23521 ttcgaaaact cgaaatggaa gggattcttt tcacacaggc gtatgctggt ggaaaacgtt
    23581 ccgaacaagg cttggcagct atttttagcg gttttccccc acatccctat acttcgataa
    23641 ccgttcaacc cgataaatat caacatcttc ccagcattgt caaaaaaata aatgcgcaag
    23701 gctatcattc ttcattttat tttggagggc agttgattta tggaaacatc aaaagctata
    23761 tcatcttcaa tcaatttgac cgtatcaagg aaatttatga tttcaaactt cccaatgtga
    23821 aggggaaact tggcatacat gacgaatata cccttaacga gcttcttcat gatcttgccg
    23881 ttgaacctgc accttttttc agcgctcttt ttacgcttag cagtcattcg ccatacgatc
    23941 agcccatgga agaaaaactc ttctggggtg ataacgaacg caattacatc aattcagcct
    24001 attacaccga ctggtgtttg ggaaagtttt tcgaacaggc acgccatcag atttggtatc
    24061 cgaatacgct ttttatcctt gtggccgatc atagccataa ttcttaccga aattggcatc
    24121 caaactcttt cgaatacaat cgaatcccta ttctctttta tggtgatgta attcgtgaag
    24181 atttacgagg aacgcaaatt gactggttgg tatcacaaac ggatttaggt actactttgc
    24241 ttgaaatcct taaaatttcc acccacgaat tttattgggg gcgcaatatg tttcactctg
    24301 gctttcatga gtttacctat ctcaccttcg attatggggt aggatg
//
LOCUS       DAOORU010000015        23997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_2566_length_23997_cov_5.559059, whole genome shotgun sequence.
ACCESSION   DAOORU010000015 DAOORU010000000
VERSION     DAOORU010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2566_length_23997_cov_5.559059"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(343..2193)
                     /locus_tag="PLF20_02155"
     CDS             complement(343..2193)
                     /locus_tag="PLF20_02155"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOX79845.1"
                     /translation="MNKHLLLIFICLIALASNAQTSDLVFLIPAGEKYEGQDVLKEME
                     RIDPDYFKAYNQLMRGFMAESFSLYDLMQNYRVHQGKISEKEPLYIAFTQHGINQACR
                     GFVLQTENGIIKKDETYYIDFDRDILDENPAKAGSITQVLPQEIGRIILSQLSGRVSQ
                     IVPKEHYFCTQTDRATAFYEGFAEHFRFISVQSEPDERIKRTIQEDLREIGVWLPKYI
                     HGFRRDYNLKGRFGVFRASAPVWYPKLEIMRNHTFIEGRLIQRPPQLSRNDDPWLQIL
                     YKDASVWPDITRYRTMNNAVATEGVIATFFSYLIASNGKKNYYPPLYYRDFLPDDSTF
                     IFERQIFPLRNEYLKIFTVLAKYVRMDVLDSRTQIIDFIEGYSKEFPDEAGLVKGIWR
                     AASGIDYQPDLPEPLWVVNNNAHFTPWVLSQFGPKLKTYPFDINNCDSVELIAVKGVT
                     PTDAVELIQYRNQKGGFQSLAQMASIPKLSPSAREGLAQLQPYQKEKISTKNPSPSWF
                     YTYTLWAFLKTAFLYFIVIGLIYFGVAQLLHYHPKPVQYLWNFLQFFLLSLLGIVCTA
                     ITTRNIMLFMGFVLVILAIQYVFRRKQGMACWFEMGTTLFMSLLLVYSLY"
     gene            complement(2487..3176)
                     /gene="lipB"
                     /locus_tag="PLF20_02160"
     CDS             complement(2487..3176)
                     /gene="lipB"
                     /locus_tag="PLF20_02160"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004306283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOX79846.1"
                     /translation="MTLHHKTLCIDLGLAEYKQSWDYQEQLCRQLQKAVLNGSYANAC
                     NYLLFVEHPHVYTLGKNGKPGNLLVNSKFLNKIGASFCMTNRGGDITYHGPGQIVVYP
                     LFYLEAFRMGVRQYIENLEEAVIRMLNDYQIKAMLMPGAIGVWLDADTPAARKICAIG
                     VRVSRGISMHGLALNVNTRLEYFNYIHPCGFVDKGVTSMEKELHSNLNIHEVKENIRG
                     KLANAFDLEFV"
     gene            complement(3178..4029)
                     /gene="lipA"
                     /locus_tag="PLF20_02165"
     CDS             complement(3178..4029)
                     /gene="lipA"
                     /locus_tag="PLF20_02165"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013760902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOX79847.1"
                     /translation="MEDYRQPKPEWIKVHIKKDQKYRQTASLLKSYSLNTICSSGNCP
                     NIKECWKYGTATFMILGEICTRACKFCNTATGKPMPPDPDEPQRLAKAAVEMGLRYIV
                     VTSVDRDDLEDLGADHWVRTIVAIRECLPQAQIEVLIPDFQGRENLIQRIIKGKPDVV
                     GHNLETIERLTPMIRSRARYNVSLEVIRQLAKSGIPAKSGIMVGLGESFEEILQTMDD
                     LLDAGCSIMTIGQYLRPSPRHYPVMEYIQPEIFSELKKIALEKGFKAAECGPLVRSSY
                     HASEYKI"
     gene            4492..5493
                     /locus_tag="PLF20_02170"
     CDS             4492..5493
                     /locus_tag="PLF20_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79848.1"
                     /translation="MKTNFFTKIVVSAFLLIIIAYGCTTTTYLIVSEGESFNALTKIT
                     DNENPCFDSDGGSEGTNLVFTVREKDGSYNIYMKDNVLSRATIQKTYGTNLNVSPAVC
                     VAKGKIAFQYYDKNNFDIYYVDAYKGKAITQVTYTDENEYNPSWNPEGTIIIFEKGAQ
                     PRSFIAVSEKNNKAAIYNLVTVTKNQIWLKNLVTGELKMIGEGSFPSISPDGNMIAFI
                     KYDLNKQKNAEVGTLWIMTIDGDSPRQLTTSDLGYAIRPKWSPDGTQIVFQLTKKNKT
                     DSDIYTIDVNGENLKQHTNNPADDFSPFWSSDGYIYFSSDRGAKKGEYQIWRFKIEE"
     gene            complement(5535..6878)
                     /locus_tag="PLF20_02175"
     CDS             complement(5535..6878)
                     /locus_tag="PLF20_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOX79849.1"
                     /translation="MKKSYLFYTLLVIIAMVSVWLANHQHNFAANFNGFTVVFCMLFL
                     DFILWLHFFGVIRKYSKKKKCILCVLFWLPFMLFVFGAVYSFLQPFGLWPPTLQTWYL
                     GSIFIVYSAKVLPVFIYVISDIIHWITGLKKLGKQKEKAFLDALLDMISSPLWVKFIA
                     RSGFSVVFIVLMFGAIVWPYRVEIKKLNFPLKDLPASFNGFRIVQISDLHLGSWPSKK
                     SLKKIVSKTNDLNPDLIVFTGDLVTFTTKEAYPFREILGKLHSEEGVITILGNHDYGG
                     YVKWASPNAKNKNLEDLFRLYQEMGWKLLLNESLVINKNGEQIAILGVENWGSQKRFP
                     QRANLEKTLAQVENIPVKILLSHDPTYWEKIVNQRDISILLTLSGHTHGMQIGIETGN
                     FRWSPAKFIYTYWSGLYKDSKTPQYLYVNQGLGCIGYPGRMGIWPEITLIELKVK"
     gene            7164..7250
                     /locus_tag="PLF20_02180"
     tRNA            7164..7250
                     /locus_tag="PLF20_02180"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7198..7200,aa:Ser,seq:gct)
     gene            7286..7360
                     /locus_tag="PLF20_02185"
     tRNA            7286..7360
                     /locus_tag="PLF20_02185"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7321..7323,aa:Pro,seq:tgg)
     gene            7542..7742
                     /locus_tag="PLF20_02190"
     CDS             7542..7742
                     /locus_tag="PLF20_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOX79850.1"
                     /translation="MKFFYQSLAQIVENHFPAVLCLVIETKGSTPRKTDSKMVVMPDG
                     KVIGTIGGGKNRISSHRESTCH"
     gene            7696..7929
                     /locus_tag="PLF20_02195"
     CDS             7696..7929
                     /locus_tag="PLF20_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79851.1"
                     /translation="MEKTEYQVIEKALAIKPGDPPLLLHFNLHPDRGLFYGGEMKIYL
                     ERLDAGTRLFIFGAGHVGKATARLASSSTSKYS"
     gene            7947..8324
                     /locus_tag="PLF20_02200"
     CDS             7947..8324
                     /locus_tag="PLF20_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HOX79852.1"
                     /translation="MFSESDQQNFHCIEGNYSEIITKLDINTNDFVVVVTHEHALDIS
                     LTAAIATLRPAYIGMTGSKSKSREARKTLQEKYYLDKELIIKIDLPIGVPIAAETPEE
                     IALSIVTALVDTIIRLNGIHPKK"
     gene            8620..9801
                     /locus_tag="PLF20_02205"
     CDS             8620..9801
                     /locus_tag="PLF20_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX79853.1"
                     /translation="MDNKELGFNSLLVHAGGVENGMNSAITPIYQTSTFTFENADHGA
                     ACFAGEESGYIYTRLGNPTITDLEKTLAILEKGYGGIATASGMGAVSTIYAGLLGAGE
                     HMVGHEAVYGSSRLIAETIFKRFGVESDWIDTSDLESVEKHIRPNTKLIYLETPANPT
                     MTIVDIAAISQMAHARNIKVCVDNTFCSPYLQNPLELGADVVLHSLTKFINGHADAVG
                     GMIITKTEADYKALRIVMTNMGPNMDPHQAWLIRRGLKTLALRMERSQANAMKVAEFL
                     ENHPKVAWVKYPGLKSHPQYELACKQMRGPGAMISFEVKGGLPAGKIVMDNVKLALLA
                     VSLGGIETLIQHPASMTHSKMSPEARHQAGISDGLIRLSVGIEEAEDIVADLSQALDL
                     I"
     gene            9978..12278
                     /locus_tag="PLF20_02210"
     CDS             9978..12278
                     /locus_tag="PLF20_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOX79854.1"
                     /translation="MRNLTYKAFMVFLLSLFSFSEVYAQKVAVVLSGGGAKGACHVGL
                     LKALEENDIPIDYIAGTSMGAIVAGLYAAGYSPEEMEVLMTSEEFKKWAKGVIDTEYM
                     YYYKKDPNDPSWVSVPVEISPKKFKPKLPTNLVSPYEMDFQFIRIFADAGAAANYNFD
                     SLFVPFRCVAADITANQPYIARSGNLENAIRASMTFPLYFKPITIDGHLMFDGGIFNN
                     FPVDVVLEDFKPDYIIGSKAAGDYSIPNPDDIVSQIEGLVMNKVPYQIPEGVNGIMVA
                     PQLIKVEVNDFSQAKAFIDSGYVATTALMDSIRRNIGRRESKEERIMKRQAFNNRKPD
                     MFIDQVIVTGVNPAQADYIRKLIVHQPRQRLFPEMKEDYYRLITDSRFRSVFPRLKFN
                     PESGYYDLTLEVERATPFELQFGGVITSSPNTEGYLQGTYYLLGENALNISLNGYFGR
                     FYLGGRLRARFDFPIKNPYFIEMDLVSSRYHYFPSGLIFLDDVLSSNLSRYDYNVTGI
                     IGWPVTNKGVFKINGILGDWETRYYHTNAVKRTDVQDKTSLEYFKLGAELDFNTLNQR
                     QYATAGSRLDACLNFGGGTEYHTPGTTSFAKQKAEFYHSFMDVKFTYDNYFAKSGPFT
                     FGMLSEFVFTTLPRFRNYTSTMILMPQFSPFPDANVRFLENLRSQSYLAGGLKILYSF
                     SDAIHLRIEGYYFQQFRKIFQNSKQGAYFGPWLSHHTYTTTSALVAHTFLGPFSLNLN
                     YFGGETSPWSFTVNFGYYLFHKKGLN"
     gene            12636..13553
                     /locus_tag="PLF20_02215"
     CDS             12636..13553
                     /locus_tag="PLF20_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79855.1"
                     /translation="MNADDLENFLNSLEHMHFKDLDELNRYAAKYFQQYNQTPQPNFE
                     GYSPTEMTYLFRNPYDDKSPMKLQKLDESDYKQIPLFNLVKYLANRIASEGELKLTQK
                     GNLPKKIVSELYDQGFIQDYFIESGLSKPYYEEKIKGIHLTRIILQLSGITKKRNNQL
                     SLTLKGNRLLSNNDELLKLIFKTFTQKFNWGYFDLYETETGQMGFAFSLFLLSKYGNS
                     KQLDNFYANKYFKAFPKLLDSFETHYLSLKNEPERCYSTRTFDRFIDYFGLIHIDTDD
                     KIIPKEKFVTKTDLLDKFIHFEPHRAICE"
     gene            13627..15900
                     /locus_tag="PLF20_02220"
     CDS             13627..15900
                     /locus_tag="PLF20_02220"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HOX79856.1"
                     /translation="MKRFISFLFAAFSIIGLSAQDNRVLMIDKGWKFIPGDHPQVFLP
                     DYDDANMKSIEVNKIWEEQGYDPLDGFCWYRLHMVIPSELKTKAILQDSLFIYLGKIN
                     NFDQTYLNGHLIGVNGFKVTDSQKDDLSFIKAPTNYWDRPRAYKLALNDSLIRWDKEN
                     IIAVRVYDEGGQGGMYTGDQFIRLTCFSDYISLECNGTPYHFNGNTLSKQIKIRNVSS
                     AYFIRGKLEIIAINKLTGHETFHQSLKVKLRPADVFDFHLELPQMDHSQVVTYRYSIK
                     SEGSDKIYRDETPYILTPPVKDTPQINGPYITGARPHHPFLFTIPVTGEKPLNFEVKG
                     LPNGLVLNNTSGIISGMVEEPGEYRLSIKVSNALGSDFREMIVKIGPDICLTPPMGWN
                     SWNCWGLAVDQEKIADAANAFVNEGLRDHGWTYVNIDDGWNIHKDAEPKRDDLGNILP
                     NEKFPDMKGLGDAIHSLGLKMGIYSSPGPYTCGQYTGSYQFEQKDAQSYASWGVDYLK
                     YDWCSYDQIARDTSLVERKKPYHVMKEALNQVNRDIAYSLCQYGMSKVWRWGAEVGGN
                     LWRTTGDITDTWGSLKSIGFSQVENQPYAGPGHWNDPDMLVVGWVGWGPNLHPTRLTP
                     DEQYTHISLWCLLSAPLLLGCDLQQLDPFTLNLLTNDEVLAINQDALGYQAKQILVEG
                     DIQVWIKKLSDGTFAIGIFNLGDKTHGYHFDFAKTGLPQQMYLRNVWKNENLGHFIKG
                     MGMKIPSHGVILLRQIP"
     gene            complement(15973..16749)
                     /locus_tag="PLF20_02225"
     CDS             complement(15973..16749)
                     /locus_tag="PLF20_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX79857.1"
                     /translation="MKTYFKYLFPIILLGFIFPTGILRGHSNPQALLDSATSSYTKGF
                     YSDAVVYYEKVLSMGFESAEVYYNLGNAYFKLNELAPAIYNYEKALMLKPGDEDILFN
                     LKIANSRITDKIDVVPEFFFQRWWESFYNFFPTDTWAILSLVFLGLFLLALGIFLLSK
                     RISYRKIGLWSAVVLIVFASFSFAFAWKGQQRQSEKQNGIIFEPAVVVKSSPDNASVD
                     LFVLHEGTKVEIQDKLDNWVKIKIANGNIGWLPASTLREY"
     gene            complement(16779..18653)
                     /locus_tag="PLF20_02230"
     CDS             complement(16779..18653)
                     /locus_tag="PLF20_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOX79858.1"
                     /translation="MKRIKTLIFILSIIVPVFSLKSQNVEFTASAPSAVSQGETFQVT
                     YKVNAKGEKFNGPDFKGFDVVSGPNVSQSSSFQFINGKMSQSNEQIYTYLIHASEVGT
                     FDIKPASIVVEGKTYYSNTLRITVEKSSSGSTSKSPSKPRTTPGRPTEPIQSIDDLSQ
                     EDIFVKAYVSKSNPYVGEQVIITYKLYTKVPLANIRINNLGTFPGFWSQDLIKSDQLQ
                     QSTEIINGQEYVTAEIKKSALFPLKSGTFNIPPLEMEATATLIKQRKRFSTGDPFFDQ
                     FFNDAFFNTGYERVNKKVYSNAITITAKPLPTSGKPSEFSSAVGNFKFSAQVDRTQLK
                     TNEALNLKVTISGSGNLDMIEAPNIKFPTEFDTYDPKVNSNISTSSSGFSGSKTFEYL
                     IIPRTPGKYTIKPFTFSYFNPSTGRYYTETSPEFTIEVLKGAEQPAGITYSGVNQQDI
                     KYIGTDIRYIKLPPFHLRPVGVTFFASKLYYGILIGLIVLFGLLIFGIKTMRKRRGDA
                     GLMRLRKATSVATKRLKTAHTYLKNQQEAQFYNEISAALWGYISDKFNIPMAELSMEN
                     VENKLLQKNVDPQTIKAFIDTLNNCEFARFAPGNKSENMEQVYKEALSTISRIEQELK
                     "
     gene            complement(18740..19423)
                     /locus_tag="PLF20_02235"
     CDS             complement(18740..19423)
                     /locus_tag="PLF20_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX79859.1"
                     /translation="MEKRYLCLLVLLLIHFSTVEAQRENRFIRKGTKEYESNKYNEAE
                     IAFRKALEKNPSSAVSQYNLGNSLYKQNNLEESVKAWSSLANRSDLSSQNSAHVWHNI
                     GNAFLKSKKYNEAIEAYKNALRLNPKDEDTRYNLSYALRMAKKQDQQKQKDQQKQDQQ
                     KQDQQKDQQKQDQQQAQQQPQQGQPQEISKQDAERMLEAMKNAEQKTLEKLKKEKKAI
                     VTEKPEKDW"
     gene            complement(19437..20468)
                     /locus_tag="PLF20_02240"
     CDS             complement(19437..20468)
                     /locus_tag="PLF20_02240"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX79860.1"
                     /translation="MFRFENPEYFYFLLIIPISVALFVVGRIIREKMLARFGEKSLLS
                     RLMPDYSNVRPILKLILWNIAMIILTIAMANPQIGTKLVKAKQKGIDMMICLDVSNSM
                     LAEDVKPNRLENAKLAIRKLVEQLQGDRIGIIVFAGKAYIQLPITSDYAAAEMFINNI
                     NTRLIPTQGTALAQAISMAKASLQSETRSKAIILITDGEDHEGDVLTEAEKAAAEGIR
                     IYTIGIGTPEGSPIPIVNEKGEITGYKKDAQGNTIITRLDEITLQKVASTGQGIYIRA
                     QNTNQALRKIFEEINKLEKTEFDTQLFSEYESWYPYFLWIAFVLLVLEMLIPARKSHW
                     AKRINLFGQ"
     gene            complement(20494..21477)
                     /locus_tag="PLF20_02245"
     CDS             complement(20494..21477)
                     /locus_tag="PLF20_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOX79861.1"
                     /translation="MTFAHPWLLLLLLTIPLLVGWYIYRGKKLHPTLSFPQTGYFSKL
                     PHTPRQRFRHIPFILRILSLIFIIIALARPQSSISRQDVSIEGIDITIALDISSSMLA
                     EDFSPNRLEAAKIVASEFVEGRPNDRIGLVAFSGEAFTQCPLTTDKTVLLELFRELKS
                     GMIEDGTAIGDGLATAINRLRESTTVSKVIILLTDGENNRGNIDPISAAEIAKKYGIR
                     IYTIGVGSRGMAPYPFQTPYGIQYQNVEVKIDENLLQQIAQMTGGKYFRATNNATLRE
                     IYHEIDQMEKSKIDVSEFHKKTDEFRPFVLVAIILLIIEFILSHTFLRVYP"
     gene            complement(21505..22503)
                     /locus_tag="PLF20_02250"
     CDS             complement(21505..22503)
                     /locus_tag="PLF20_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79862.1"
                     /translation="MNKKIPFKEIIILTLSLLLAGIQLRAQTGEAKATVQIDSTRLMI
                     GQQVRMNISFTHPPKAKIGWGIRGDTLSRQIEIIKKSAIDSTVTPDGMVKRSQNLVIT
                     SFDTGYIVVQPIRFYWQLPQDTITQIAETEPILLEVMAMPVDTTQDIRDIKGIQKIGY
                     SWKEILPWIGYALLIAIVAFLIYRYVQYRRGKRKYFLIAPKPKIPAWQIALEALDTLR
                     NKQLWQSGHYKQFYSELTDIFRDYLDNQFQINAPEMVSEEIIIALENAGFDTKLISDC
                     RRMLQTADLVKFARYIPLPDENSFSFDVCRRFVEETMPKPENASEPHPSSPSEMQQ"
     gene            complement(22609..23484)
                     /locus_tag="PLF20_02255"
     CDS             complement(22609..23484)
                     /locus_tag="PLF20_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOX79863.1"
                     /translation="MDTSEIFKKVRKIEIKSRGLSNQIFSGEYHSSFKGKGMEFSEVR
                     EYQYGDDIRNIDWNVTARLNHPFVKVYEEERELTVILLIDVSGSNEFGSRQRFKQELM
                     TEIAAVLAFSAISNNDKVGVIFFSQGIEKFIPPKKGTTHILRIIRELIDFKPKHSNTN
                     IAEALRFFTNAIKKRSIAFLISDFEDQGFDKALEVASRKHDVVALRVYDEREDQLPNI
                     GLVEAKDPETGEITWIDTSNSQIRERYANMRKHHQRYLRDVFTRCGIDNISIHTAQDY
                     VPPLIRLFQIRERRK"
     gene            complement(23529..>23997)
                     /locus_tag="PLF20_02260"
     CDS             complement(23529..>23997)
                     /locus_tag="PLF20_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOX79864.1"
                     /translation="DRFMLKVVITYPDKEEEKLILRQNIRNEEVFTETRLKPEDIIKA
                     RSLVREVYLDEKIENYITDIVFATRFPEHYKIDSFKGLISYGASPRASINLALASKAF
                     AFIRRRGYVIPEDVRAVAHEVMRHRIGLTYEAEAENITTEEIISQILNTVEVP"
BASE COUNT         7312 a         4717 c         4637 g         7331 t
ORIGIN      
        1 ggaaaaaatt tgtgtttccc gaaagttgaa agttaaagat tcaagttgct gtataaaagg
       61 aaattgcatc cctaaggctc tcaagttggg ttaagcggcg aatacgtgat gagcttgttt
      121 ggtgatactg ctaattttga gtattggcac gatttgaata aagtgtacct ggaagataaa
      181 atggatgata tttaaattgg gaaaagactt aacaggtctt ggagacctgt tagatctcaa
      241 aggaacgaac gaaaaagtaa taagagctca caggtcttac aggcctttta aacatcctaa
      301 tactttttgt tcaaatttca ataaaatata acttctataa cttcaataca gtgagtaaac
      361 gagcaacaaa ctcatgaata aggtggttcc catctcgaac caacatgcca taccctgttt
      421 tcgacgaaag acatattgaa tcgcaagaat taccaacaca aaacccataa acaacatgat
      481 gtttcttgta gttatggccg tacaaactat tcccaatagc gaaagcaaaa agaactgaag
      541 aaaattccaa agatattgta caggtttggg atgataatga agaagctgag ccacgccaaa
      601 atagatcaaa ccaatgacaa tgaaataaag aaatgctgtt ttgagaaaag cccagagcgt
      661 ataggtatag aaccagcttg gcgaaggatt tttagtgcta attttttctt tttgataagg
      721 ttgtaattgt gcaagcccct ctcgggccga aggactgagt tttgggatgg aagccatctg
      781 agcgagtgat tgaaaaccgc ccttttgatt tctgtattga atcaattcta cagcatccgt
      841 tggtgtaact cctttcacgg ctatgagttc aactgaatcg caattattta tatcgaaagg
      901 ataagttttt aatttaggtc caaattgtga gagaacccat ggagtaaaat gagcattatt
      961 atttacaacc caaagaggtt caggtaaatc aggttgataa tctatgcctg aagcagccct
     1021 ccatatcccc tttaccaatc cggcttcgtc aggaaattct ttggaatatc cttcgatgaa
     1081 atcgataatt tgagttctcg aatccaatac atccattcgc acatattttg ccaaaaccgt
     1141 aaaaattttc aaatactcgt tgcgcaaggg aaatatctgg cgttcaaaaa taaaagtcga
     1201 gtcatcgggt aaaaaatctc tgtaatacaa aggaggataa taatttttct tgccgttgga
     1261 tgcaatcaaa taggagaaga aagtggcgat gacaccttcg gtagccactg cattgttcat
     1321 tgttctataa cgagtgatat cgggccatac gctggcatcc ttgtataaaa tttgtaacca
     1381 gggatcgtcg tttcggctta attgaggtgg acgctgaatc aacctgccct caataaatgt
     1441 gtggttccgc atgatctcga gttttggata ccacaccggc gcacttgcac gaaacacacc
     1501 aaaacgccct ttcaggttgt aatccctcct gaatccatgg atatatttag gaagccaaac
     1561 accaatttcc cttaaatctt cttgaatggt tctcttgatt ctttcatcgg gttcggattg
     1621 aaccgaaata aatctgaaat gttcggcaaa tccttcataa aatgccgtag ccctatcggt
     1681 ttgcgtacaa aaataatgct ccttgggcac tatctgactc actcttcccg agagctggga
     1741 taaaattatc cgaccaatct cctgaggcaa aacctgagta attgatcccg cctttgccgg
     1801 attctcatcc agaatatcgc gatcgaaatc aatgtaataa gtttcatcct tcttaattat
     1861 tccattctcc gtttgcagaa cgaatcccct acatgcctga ttaatcccgt gctgcgtaaa
     1921 agcaatgtat aaaggttctt tttctgaaat ttttccttga tgaacccgat aattctgcat
     1981 cagatcgtaa agagaaaaac tctcggccat gaaacctctc atcaattgat tatatgcttt
     2041 aaaataatca gggtctatgc gctccatctc cttaagtaca tcttgcccct catatttctc
     2101 tccagccggt atcaaaaaaa cgaggtcgga agtttgggca ttagatgcca aagctattaa
     2161 gcatataaaa attaataaaa gatgcttatt cataacttat tgatttataa taaaataata
     2221 ttcggtaatc attttgcaat tttatgcaaa tttttagcaa cacgaattta aaagaacaac
     2281 tgcttgttca cagaaagact tcaaatccat ttattttagc ataaatattt caaaaccaat
     2341 aaaatacttc atttcgattt gtttactcct gataaaataa tccgatcaat cgtcattcga
     2401 tcgagcgaca caagcagaca aggttcgagc taaaaaactt ccactatatc gcggtaagtt
     2461 taaaattttc gagtaataaa gaaacttcaa acaaattcca ggtcaaaggc attggcaagt
     2521 tttcctctta tattttcttt cacttcgtgg atattaagat tggaatggag ttctttttcc
     2581 atggaggtca cacccttatc tacaaatccg caaggatgaa tgtagttaaa atattcgagt
     2641 cgagtattta cattaagtgc cagcccatgc atgctaattc ctctgctaac cctaacccct
     2701 attgcacaga ttttacgtgc agcgggtgta tcagcatcca gccatacacc aatagctcca
     2761 ggcataagca tggcttttat ttggtaatca ttcagcatac ggatcacggc ttcttcgaga
     2821 ttttcgatat actgcctcac acccatccga aaggcttcga ggtaaaataa agggtaaacc
     2881 acaatttgac ccggtccatg atatgtaata tctcctcccc tattggtcat acaaaaactt
     2941 gctccgattt tgtttaaaaa tttactattt accagcaaat tgcctggttt tccatttttc
     3001 ccgagtgtat aaacatgggg atgttcaaca aacagcaagt aattgcaagc attggcatac
     3061 gaaccattta aaacagcctt ttgcaattgg cggcacaatt gttcctgata gtcccagctt
     3121 tgtttatatt cagccaatcc cagatcgata caaagtgttt tgtgatgcag tgtcatttca
     3181 aattttatac tcagatgcat gataggatga acgcaccaaa ggtccacatt cagcagcctt
     3241 gaatcccttt tcgagagcaa ttttttttag ctcactaaaa atctcaggtt gaatatattc
     3301 catcacagga taatgccgag gagaaggtct cagatattgg ccgatggtca ttatagaaca
     3361 acccgcatcg aggagatcgt ccatagtttg taatatttct tcgaaagatt cgccgagacc
     3421 caccataatt cccgatttgg ccggtatgcc acttttggct aactgacgga ttacctcgag
     3481 gcttacatta tatcgagcac ggcttcgaat cataggagta agccgctcta tagtttcaag
     3541 attatgaccg acgacatcgg gttttccttt aataattctt tgaatcaggt tctccctgcc
     3601 ttgaaaatcc gggatcaata cttctatttg tgcctggggt aggcattcgc gtatggcaac
     3661 aatcgtccta acccaatgat ctgctcccaa gtcttcgagg tcatccctgt caacagaggt
     3721 gacgacgata tagcgtaaac ccatctctac ggcagcctta gctaatcgct ggggttcatc
     3781 gggatctggg ggcatgggtt tacccgtcgc cgtgttgcaa aatttacagg ccctcgtaca
     3841 aatttccccc aaaatcataa aagtagctgt cccgtatttc cagcattctt taatgttggg
     3901 acagtttccg ctgctacaaa tggtattaag tgagtaagat ttcaacaagg aagcagtttg
     3961 gcgatatttc tgatcctttt tgatatgaac ttttatccat tcaggttttg gttggcgata
     4021 atcttccata ataaagttaa atacgtcgtt taaggctgca aaaataaatc aaagtaaact
     4081 ggattgtgaa agaatagatt ttagaatcca agcctaattg taattcagta aaaacaaaat
     4141 tttcaaaacc ccttaaatat gagccgaatc tttagagaat gattaagatt cgcaaatttc
     4201 tctctttaag gaaagaaaga aattcggttt acttcagccc aatgacctga taataatttt
     4261 ggaggtactt taatccctca ctgaaacgtc aacaaaagaa ctatctcaga aataattttt
     4321 tcgatttata attcaaaatc agctttttac gattaaaatt gagaaactta taaaacaaag
     4381 atttagggat tattaaattt ttactacaaa ttactttatg atgataatac tctccaatat
     4441 tgttataaat ttgtttctaa aatcaattca ccaaaaacat taccccaagc catgaaaaca
     4501 aactttttta ctaaaatcgt tgtttctgct tttctcctga taattattgc ttatggatgt
     4561 accactacaa cctatttaat cgttagcgaa ggagaatctt tcaatgccct gacgaaaatc
     4621 actgacaacg aaaacccctg ttttgattct gacgggggaa gtgaaggaac taatttggtt
     4681 tttaccgtta gagaaaagga tggttcttat aacatttaca tgaaagataa tgttttgtcg
     4741 agagccacca ttcaaaaaac ttacggaaca aacctaaacg tatcgccagc agtttgtgta
     4801 gctaaaggga aaatagcttt tcagtactac gataagaata atttcgatat ttactatgta
     4861 gatgcttata aaggcaaagc cattacgcag gttacctata ctgatgaaaa tgagtacaat
     4921 ccttcgtgga atcctgaagg tacaatcatt atttttgaaa agggggcaca accacgttct
     4981 tttatagcag tatctgaaaa gaataataaa gctgccattt ataacttggt tactgttaca
     5041 aaaaaccaga tttggcttaa aaatctcgta acaggagaat tgaaaatgat aggtgaagga
     5101 agtttccctt caatatcacc cgatggaaac atgattgcat tcattaaata cgatttgaac
     5161 aaacaaaaga atgcagaagt gggaactttg tggatcatga ctattgatgg agattctcct
     5221 cgccagctta ctacttcaga tttgggatat gctattcggc ctaaatggag ccccgatgga
     5281 acgcaaattg tcttccaact caccaagaaa aacaaaaccg actccgacat ttataccata
     5341 gatgtaaatg gtgaaaattt aaaacaacat actaataacc ctgctgacga tttctctcct
     5401 ttctggtcaa gtgatggata tatttatttt agctccgacc gtggcgctaa aaaaggtgag
     5461 tatcaaatat ggagattcaa aatagaagaa taacactact caaaatggct aaaataaatt
     5521 agatatttaa atgcttactt aactttcaat tcaattaaag tgatttcagg ccatatcccc
     5581 atcctcccgg gataaccaat acatcccaaa ccttgattaa catagagata ttgcggggtt
     5641 ttactatctt tatataaacc cgaccaatat gtgtaaataa attttgcagg gctccagcgg
     5701 aaatttccgg tttcgatacc aatttgcatt ccatgagtat gtccggaaag cgtaagaaga
     5761 atagatatat ctctctgatt cactattttt tcccaataag ttggatcgtg tgaaagaaga
     5821 atcttaacag gaatattttc aacttgagcc aaagtcttct caaggttggc tctttgggga
     5881 aatcttttct gactgcccca gttttctact cccaaaattg caatctgttc cccattctta
     5941 ttaatgacca aagattcatt aagaagcagt ttccagccca tttcttgata taaacggaat
     6001 aaatcttcca gatttttatt ctttgcatta ggtgaagccc atttcacata tccaccgtaa
     6061 tcatgattgc ctaaaatggt tattacgcct tcttctgaat ggagttttcc caaaatttca
     6121 cggaaaggat aggcttcttt agttgtgaag gtaaccagat cacccgtaaa tacgatgaga
     6181 tcaggattca agtcattcgt cttgcttact atttttttca atgatttctt tgaaggccaa
     6241 ctgcccagat gcaggtccga aatctgtacg atgcgaaatc cattgaatga ggcaggtaaa
     6301 tctttaaggg gaaaattcag tttttttatt tcgactcgat atggccagac gatggcccca
     6361 aacatcaaaa cgataaaaac aaccgaaaaa ccggatcgtg caataaactt tacccataag
     6421 ggcgatgaaa tcatatcgag caaagcatct aaaaaagctt tctccttttg tttgcctaac
     6481 ttttttaatc cggtaatcca gtgtatgatg tcagaaatta cataaatgaa aaccggcaat
     6541 acttttgcac tataaacaat gaaaatcgat cctagatacc atgtttgcag cgttggcggc
     6601 cataacccga aaggttgcag gaatgaataa actgcgccga aaacgaacag cataaaagga
     6661 agccagaaaa gaacgcacag gatacatttc ttcttcttgc tatattttcg aatgacaccg
     6721 aaaaaatgca accagaggat aaaatcgaga aacagcatgc agaaaactac cgtaaatcca
     6781 ttgaaattag ctgcgaaatt atgttgatgg tttgccagcc atacactcac catggcaatt
     6841 ataaccaaaa gagtataaaa taaatatgat tttttcaaat agatatgaga attttgtttt
     6901 tcatataact cccggcacaa aattacaaac cttcccagaa ttataaccgg caaaagagtt
     6961 gaatgagtct ttatctgaga cgaaaagaaa atatgaaatt cactgaagct tttgatataa
     7021 atgaaatttt ttatactttt gcagcttcaa tcagaaacgt ggtctttcta aaaatacaag
     7081 gagaagggga agcgttcttt tgatgaatga agtctgaaag attcgggttt tgaaacttga
     7141 acatcaataa gccaaaaaaa tccggtgagg tgggtgagag gcttaaacca gcagtttgct
     7201 aaactgccgt acgggtagcc gtaccggggg ttcgaatccc cccctcaccg cagttggatt
     7261 catccatcaa aattttaagg gctttcgggg tgtagcgcag tctggttagc gcacctggtt
     7321 tgggaccagg gggtcggtgg ttcgaatcca ctcaccccga ctacattagc cttaatgaac
     7381 aaatcttatc cagtccatcg catggatcct gtacctttaa ataacaatct tcctttattt
     7441 tccgaaaaaa gaatccagta accagaaatt tcctgtattt aaggcttgtc ctaagaataa
     7501 atcacgagcc ctgatattgc ttatttttga taaaaaatgc catgaagttt ttttatcaat
     7561 ctttggctca aatagttgaa aatcattttc cggctgtatt atgtcttgta atcgaaacca
     7621 aaggttcgac gcctcgcaag accgatagca aaatggttgt gatgcccgac ggaaaagtca
     7681 taggaaccat aggaggtgga aaaaacagaa tatcaagtca tcgagaaagc acttgccatt
     7741 aagccaggcg accctcccct cttacttcat ttcaaccttc atcccgaccg aggactgttc
     7801 tacggtggag aaatgaaaat ctatctcgaa cgtcttgatg caggcacccg tctattcatc
     7861 ttcggagcag gccatgttgg aaaagccact gcacgactcg cttcctcttc gacttccaaa
     7921 tattcctaat cgatgaaaga ccaaatattt tctccgaatc cgaccagcaa aattttcatt
     7981 gcatcgaggg aaattacagc gagatcatca caaaattaga tatcaataca aatgactttg
     8041 tagtagtagt tactcatgaa catgccctcg atatttctct cacagcagcc atcgccactt
     8101 tacgaccagc ctatattggc atgacaggtt cgaaatcgaa aagccgggag gcacgaaaaa
     8161 ccttacaaga aaaatattat ctggataaag aactcatcat aaaaatcgat cttccgattg
     8221 gtgtacccat agccgccgag actcccgaag aaattgctct ttctattgtt acagccttag
     8281 tggatactat aatccgcctt aatggcattc accctaaaaa ataacaaaac aatgcattgg
     8341 tcttgttttc gaaaaggcca aaaaatatcg aagtgcgagg tgagacatgg agaatggcgg
     8401 atagaaaact gcctacgaga aatggaaaat ggaagaggct tgagacgaaa agtcaaggag
     8461 acggagaaat gtcaatattc acataatcct atattgagta gatcacatga gaattcttaa
     8521 taaggcgaat atcatcctct tccgtttcat taagttaaat tgacaatgaa tagttatacg
     8581 ggatgaaatc cttacctttg cagcaaaatt cgataattta tggataataa ggaattaggt
     8641 ttcaattctc ttttggttca tgccggagga gttgaaaatg gaatgaatag tgctattacg
     8701 cctatatatc aaacttccac atttactttc gagaatgccg atcatggggc agcatgtttt
     8761 gcgggggaag aatcggggta tatctatacc cgtttgggta accccaccat taccgatttg
     8821 gaaaaaacct tagccatact cgagaaagga tatggaggta ttgccacggc ttcgggcatg
     8881 ggtgctgtaa gtacaattta cgcaggttta ctcggtgcgg gcgagcacat ggtcggtcat
     8941 gaggccgttt atggttcatc gcgcttgatt gccgaaacca ttttcaaacg ttttggcgtc
     9001 gaaagcgact ggatcgacac ttctgatctg gaatctgtag aaaaacatat acggcctaat
     9061 acaaaactta tttatttgga aaccccggcc aatccaacaa tgaccattgt agatattgcc
     9121 gcaataagcc agatggctca tgccagaaac atcaaagttt gcgtggataa cactttttgc
     9181 agcccttatc tgcaaaatcc tctcgaatta ggtgccgatg tcgttttgca tagtctgacc
     9241 aaatttatca atggacatgc cgatgccgta ggaggaatga taattaccaa aaccgaagcc
     9301 gattacaaag ctttacgcat tgtgatgacc aacatggggc ccaacatgga tcctcatcaa
     9361 gcctggctga tcagaagagg tttgaaaact ttagccttac gcatggaacg ttctcaggcc
     9421 aatgccatga aagtggccga atttctggaa aatcacccca aagtagcttg ggtgaaatat
     9481 ccaggtttga aatctcatcc acaatatgaa ttggcttgta aacaaatgag aggtcctgga
     9541 gcaatgatta gtttcgaggt aaaaggaggc ctacccgccg gtaaaatcgt gatggataat
     9601 gtaaaacttg ccttattggc agtgagtttg ggcggaatag aaacgcttat tcagcacccc
     9661 gccagcatga ctcattcaaa aatgagcccc gaggctcgac atcaagccgg aatcagtgat
     9721 ggcctgattc gtctttcggt tggaatagaa gaagcagaag atattgtggc cgatctctct
     9781 caggctctcg atttaatttg aattccccat cttcttcgaa acttcatccc tcgatggctc
     9841 ttgaagatga gaatttttca tgaatttcga gcggacattc aattcgtgtt ttttgaaaag
     9901 catcatatag ttttgtgtaa ctgctctgca tcatcatatc aactatatag cctaattttg
     9961 gaaaagaatt tttttcgatg cgaaatttga catataaggc ctttatggtt tttcttttat
    10021 ccctatttag tttttctgag gtttatgccc agaaagtggc cgtggtatta agtggaggtg
    10081 gagcaaaagg agcatgtcat gtaggattgc tcaaggcttt ggaagaaaat gacattccca
    10141 ttgattatat tgccggaacc agcatgggag ccatagtagc cgggttatat gcagcgggct
    10201 attctcccga agaaatggaa gtccttatga catccgaaga attcaagaag tgggcaaaag
    10261 gagttatcga tactgaatat atgtattact acaagaaaga tcccaatgat ccttcatggg
    10321 tttcagtgcc tgtggaaatt agtcctaaga aatttaaacc caaactaccc accaatctgg
    10381 tttcacctta cgaaatggat tttcagttta taaggatttt tgccgatgcc ggggcagcag
    10441 caaattacaa ttttgacagt ctctttgttc cctttcgttg tgtagctgcc gatattactg
    10501 ctaatcagcc ctatattgcg cgcagtggaa atctcgaaaa tgccattcgg gcttccatga
    10561 cctttccatt atatttcaaa ccgattacta ttgatggcca tcttatgttt gatggaggaa
    10621 ttttcaataa ttttccggta gatgtggtac tggaggattt caaacccgat tacatcatag
    10681 gttcaaaggc tgccggcgat tattccatcc ctaatcccga tgatatcgtt tcgcaaatcg
    10741 aaggtctcgt catgaataaa gtgccctatc aaattcccga aggagtgaat ggaatcatgg
    10801 tagcacctca gctcatcaag gttgaagtga atgactttag tcaggccaaa gcttttatcg
    10861 acagtggata tgtagcaact acggctttga tggacagcat aaggagaaat atcggccggc
    10921 gagaaagcaa ggaagagagg ataatgaaac ggcaggcctt caacaataga aaacccgata
    10981 tgttcatcga tcaggttatt gtaacgggtg tcaatcctgc tcaggccgat tatatacgca
    11041 aattgatcgt tcatcagccc agacagcgcc ttttccccga aatgaaagaa gattattacc
    11101 ggttgataac ggatagcaga ttccgaagtg tatttccgcg actgaaattt aaccccgaaa
    11161 gtggttatta tgatttgacg ctcgaagtgg aaagagctac acccttcgaa ctgcagtttg
    11221 gtggagtaat tacttcatca cccaataccg aagggtattt acagggaaca tattaccttt
    11281 tgggtgaaaa tgccttgaat atttcgctga atggatattt tggacgtttt tatctgggtg
    11341 gccgcctgcg agcacgtttc gatttcccca tcaaaaatcc ctattttatc gaaatggatt
    11401 tggtttcatc tcgatatcat tattttccca gtggattgat ttttctagat gatgttttat
    11461 cgtctaatct gtcacgttac gattataatg tcacgggtat aataggatgg cctgttacca
    11521 ataaaggcgt cttcaaaatc aatggaatat taggagattg ggaaacacgt tactaccata
    11581 ccaacgcagt gaaaagaacg gatgttcagg ataagacctc tctggaatat tttaaattag
    11641 gagccgagct cgacttcaat accctgaatc aacggcaata tgctacagct ggctcgagat
    11701 tggatgcctg cctcaatttt ggcggcggaa ccgaatacca cacacctgga actacttcct
    11761 tcgcaaaaca aaaagcggag ttctatcact cgtttatgga cgtaaaattt acttacgata
    11821 attatttcgc aaaatcagga ccattcacct ttggcatgct ctccgaattc gtgtttacaa
    11881 ccctgcctcg attccgcaac tatacttcta caatgattct catgccacag tttagtcctt
    11941 tccccgatgc caacgttcgt tttctcgaga atttgcgcag ccagtcgtac cttgccggtg
    12001 gattgaaaat tttatattca ttcagcgatg ctattcatct acgaatagaa ggatattatt
    12061 ttcagcaatt caggaaaatt tttcaaaaca gtaagcaagg cgcttacttt ggcccctggt
    12121 taagtcacca tacctatacc acgacttcag ctctggtagc acatacattt ttaggccctt
    12181 ttagcttgaa tctgaattat ttcggcggag aaactagccc atggagtttt acggtcaact
    12241 tcggttatta tctttttcat aaaaaaggat taaattgaga gtatgggagc atgttttctc
    12301 aatataatgc tctaaaagtc gaagttagga aaagttgaaa aatagggaag aaaaattatt
    12361 atctgggtgt tgtggagcgg agctccacaa cacccagatt tatttatcac tcaccaatag
    12421 catatcaagg catatcgaga cgacctgttt ttctcctatt caaaaaatgc ttgaattgaa
    12481 tttcacattt ggtgaaatgt tttaatttta ctcattgaga ttgaagttca tcattatgac
    12541 aaaaaaattc caaaacatgc cattgttgta actcttctct tttccgaaca tattgtttat
    12601 taatgaatta gaaattaatt aaaaattata aaactatgaa tgccgatgat ttggaaaact
    12661 tcctgaactc tttggagcac atgcacttta aagacttaga tgaacttaat aggtatgctg
    12721 ccaaatattt tcaacaatac aaccaaactc cccaacctaa ttttgagggt tattcaccta
    12781 cggaaatgac gtatcttttt cgtaatccat acgatgataa atctccaatg aaattgcaaa
    12841 agcttgatga atcggattat aaacaaatcc cattattcaa tctggtgaaa taccttgcaa
    12901 atcgaattgc ttccgaaggt gaactcaagc tgacgcaaaa gggaaattta cctaaaaaaa
    12961 ttgtaagcga attatacgac cagggtttta tccaagatta ttttatcgag agtggtttat
    13021 cgaaacctta ttatgaagaa aaaattaaag gtattcatct taccagaata atattacaac
    13081 tttctggaat tacaaaaaaa agaaataatc aattaagcct caccctaaag gggaatcgac
    13141 ttttatcgaa taacgatgaa ttgttgaaat taattttcaa aacttttact caaaaattta
    13201 attggggcta ttttgattta tatgaaacgg aaactggcca aatgggattt gctttctcct
    13261 tgttcttact ctccaagtat ggaaattcta aacaacttga taatttttat gcaaacaaat
    13321 attttaaggc atttcccaag ttattggatt ctttcgagac ccactatctt tctttaaaaa
    13381 atgaacctga gcgatgttat tctactagaa ctttcgacag atttattgat tattttggat
    13441 tgattcatat tgataccgat gataaaataa ttccaaagga aaaatttgtt accaaaactg
    13501 atttgttaga taaattcata cattttgaac ctcatcgagc tatatgtgaa tgatcgtttt
    13561 tgaataatta atcaaatctt cttaattctc gaggaaataa atattcaatt ttcaattctt
    13621 taaataatga aacgttttat ttcttttcta tttgcagcat tttccattat cggtctgagt
    13681 gcccaggaca atcgagtttt gatgatcgat aaaggatgga aatttatccc aggagatcat
    13741 ccacaagttt ttttacctga ttatgatgat gctaacatga aatccatcga ggtaaataaa
    13801 atttgggaag aacaaggtta tgacccgctg gatggatttt gttggtatcg tttgcatatg
    13861 gttattccat ccgaattaaa aactaaggct attttacaag atagcttatt catctatctt
    13921 ggcaagatca acaattttga tcagacttat ctaaatggac atttgattgg tgtgaatggc
    13981 tttaaagtta ctgacagcca gaaagatgat ctttccttca ttaaggctcc taccaattat
    14041 tgggaccgac caagagctta taagctcgct ttgaatgatt ctttaatccg atgggataaa
    14101 gaaaatatta ttgcagtgag ggtatacgac gaaggcggac agggtggcat gtacacaggc
    14161 gatcaattta ttcgtctaac ctgtttctcc gattatattt ctctcgaatg taacggtact
    14221 ccttatcact tcaacggaaa taccctttca aagcagatca aaatccgaaa tgtttcatcc
    14281 gcatacttta tcaggggtaa attagaaatc atagctataa ataaacttac gggtcatgaa
    14341 acttttcatc agtcattgaa ggtaaaactc aggcctgccg atgttttcga ctttcatctc
    14401 gagctccctc aaatggacca ttctcaggtg gtaacttatc gttattccat aaaaagtgaa
    14461 ggatccgaca aaatctatcg tgacgaaact ccctatatct tgacacctcc ggtaaaagat
    14521 actcctcaga taaacggtcc ttatatcacg ggtgccaggc cgcatcatcc ttttttattt
    14581 actatcccag ttaccgggga aaaaccctta aattttgaag tgaaaggctt gccaaatggc
    14641 cttgttctga acaataccag cgggattatt tctggaatgg tcgaagaacc cggcgaatat
    14701 cgcttgagta ttaaagttag caacgctttg ggatcggatt ttagggaaat gattgtcaaa
    14761 attggacctg acatttgcct cactccgccc atgggatgga atagctggaa ttgttggggc
    14821 ctggccgtcg atcaggaaaa gattgcagac gctgccaatg cctttgtcaa tgaaggttta
    14881 cgtgatcatg gctggactta tgtgaacatc gacgatggat ggaatataca taaagatgca
    14941 gaacccaagc gtgatgactt gggtaatatt cttcccaacg aaaaatttcc cgatatgaaa
    15001 ggtttgggcg atgcaatcca ttctttgggc ttgaaaatgg gaatttacag ttcgccggga
    15061 ccttatacct gtggtcaata cacaggaagc taccaattcg aacaaaaaga tgcccaaagt
    15121 tatgcctcat ggggagtgga ttatttgaaa tatgactggt gttcatatga tcagattgca
    15181 cgcgatactt cacttgttga acgtaaaaaa ccatatcacg taatgaaaga agcactaaat
    15241 caggtgaata gggatatcgc gtacagttta tgccagtatg gtatgagtaa ggtatggcgt
    15301 tggggggcag aagtaggggg aaatttatgg cgtacgacag gcgatattac cgacacatgg
    15361 ggaagcctga aatcaattgg attctctcag gtcgagaatc aaccttatgc agggccaggg
    15421 cattggaatg atccagatat gctcgtagtg ggatgggtag gatggggacc aaatcttcat
    15481 ccgaccaggc taacacctga tgagcagtac acccatatct cgttgtggtg ccttctttcc
    15541 gctcccttat tgctaggctg cgaccttcag cagctcgatc catttaccct aaatcttttg
    15601 accaatgacg aagttttagc tatcaatcaa gatgctttgg gttatcaggc aaaacaaata
    15661 ctcgtggagg gagatattca ggtttggatt aaaaaacttt cagatggcac atttgccata
    15721 ggaattttca atctgggcga taaaacccac ggctatcatt ttgattttgc caaaacaggc
    15781 ctgcctcaac aaatgtatct acgcaatgtc tggaaaaatg aaaatttagg gcattttatc
    15841 aaagggatgg gaatgaaaat accttcgcat ggagtaattc ttttgcgcca aattccataa
    15901 taataacctt tcctatcatc aagggacttt tcaggttttt gctattctaa tttcaaatga
    15961 tcaacactaa gattaatatt ctctcaacgt agaagcgggg agccatccta tattcccatt
    16021 agcaatctta atcttaaccc aattgtccaa tttatcctgt atctcaactt tagtgccttc
    16081 gtgtaaaaca aataaatcta cactggcatt atcaggcgaa cttttcacca ccacagcagg
    16141 ctcaaagata attccatttt gtttttcact ttgacgttgt tgccctttcc atgcaaaagc
    16201 aaaactaaat gatgcgaaaa caatgagtac aactgctgac cataaaccta tctttcgata
    16261 acttatcctt ttgctgagga gaaaaatgcc aagagctaat aaaaaaagtc ctaaaaaaac
    16321 aagactgagt atagcccatg tatcagtcgg gaaaaaatta taaaatgatt cccaccacct
    16381 ctggaaaaaa aattccggaa ccacatctat cttatcggta atccggctgt tggcaatctt
    16441 aagattgaag agaatatctt catcgcccgg ctttaacatc aaagcctttt cataattgta
    16501 aatggcaggc gccaattcat tcaatttaaa ataagcattc cctaaattat agtaaacctc
    16561 ggccgattca aaacccatgg atagaacttt ctcgtaataa accaccgcat cggaataaaa
    16621 gccttttgta taagacgaag tggctgaatc aagcaatgcc tgtggattgc tatgtcctct
    16681 caagattcca gtcggaaaaa taaatccaag taaaatgatt ggaaataaat atttaaaata
    16741 tgttttcata aaatggtcta tctaatttat tatgaacttt attttaattc ctgctcaata
    16801 cgtgagatag tggaaagagc ttctttgtaa acctgttcca tgttctcgga tttgtttcca
    16861 ggagcaaaac gggcaaattc acaattattg agcgtatcga tgaaagcttt tatagtttga
    16921 ggatcgacat tcttctgaag aagtttattt tcaacatttt ccatcgaaag ttctgccata
    16981 ggaatgttga atttgtcgga gatatagccc cacaaggcag cggatatctc attgtagaac
    17041 tgggcttcct gctgattttt gagataggta tgagccgtct tcaaccgctt ggtagccaca
    17101 ctagtagctt tgcgcaaacg catcaacccg gcatcccctc tgcgctttcg catggttttt
    17161 atcccaaata tcaagaggcc aaacaatact attaagccaa ttaatattcc ataatagagt
    17221 ttggaagcga agaaagttac tccaacaggt cgcagatgaa agggaggtaa tttgatataa
    17281 cgaatatcgg ttccaatata tttaatatct tgctgattta ctccactata ggtgattcct
    17341 gcaggctgtt ctgcgccttt gagcacttct atggtaaatt caggcgaggt ttcggtataa
    17401 tatcttcctg tcgaaggatt aaaatagcta aaggtgaaag gttttatagt atattttcca
    17461 ggtgttcgtg gtataatgag gtattcgaag gttttgctac ccgaaaaccc cgaagaagaa
    17521 gtagagatgt tggaattaac ctttggatcg taagtatcga attcggtagg gaatttgata
    17581 ttgggagcct ctatcatgtc aagattccca ctgccactaa tagtaacctt gagattgagc
    17641 gcttcattgg tttttaactg agtacgatcg acctgagccg aaaatttgaa gttacccaca
    17701 gctgaactaa attcggaagg tttaccgctt gtgggtaaag gcttagcagt gatggtaatg
    17761 gcattactgt aaactttctt attgacgcgt tcatagcctg tattgaaaaa agcatcgttg
    17821 aaaaattggt caaaaaaagg atcgcctgta gaaaaccgtt tgcgctgttt aattaaggta
    17881 gcagtcgcct ccatttcgag tggaggtata ttaaaagttc ctgattttaa agggaaaaga
    17941 gcactctttt taatttctgc ggtaacatat tcctgcccat tgataatttc ggtagattgc
    18001 tgcaattggt cgcttttaat aagatcctgt gaccaaaatc caggaaaggt tcccaaatta
    18061 ttgatgcgaa tattagcaag aggaactttg gtataaagtt tataagtaat aattacctgt
    18121 tcgccaacat aaggattaga tttgctaaca taagccttta cgaagatatc ctcttgtgag
    18181 aggtcgtcaa tgctttgaat gggttcggta ggcctgccag gtgttgtgcg cggtttagat
    18241 ggactttttg aagtgctccc actcgaagat ttttctaccg taattcgcag tgtattcgaa
    18301 tagtaagtct ttccttccac tacaatgctt gctggcttta tatcaaaagt tcctacttcg
    18361 gaagcatgaa tgagataagt ataaatttgt tcgttcgatt gactcatttt accattgatg
    18421 aactgaaaac ttgaactctg tgagacattc gggcccgaaa ctacatcaaa tcctttgaaa
    18481 tcagggccgt tgaacttttc ccctttagcg ttgactttgt aagtaacctg aaacgtttca
    18541 ccttggctta cggcagaagg agccgaagcc gtaaattcaa cattctgact ttttagcgaa
    18601 aaaaccggaa caatgataga caaaatgaat atcagagttt ttattctttt catgtttggg
    18661 tcgagctttc tatttttaat aaaattatat cacaaacgta agtaattcag ccggaaacgg
    18721 tggcacaaaa tttatgcctt taccaatctt tttctggttt ttcggtaact atagctttct
    18781 tttctttttt aagtttttct aaagtttttt gttcggcatt tttcatggct tcgagcatac
    18841 gttcggcatc ttgtttcgaa atttcttgtg gttgaccttg ttgaggctgc tgttgggctt
    18901 gttgctgatc ttgcttctgc tgatcttttt gttgatcttg cttttgttga tcttgcttct
    18961 gctgatcttt ttgcttctgc tgatcttgtt tctttgccat tctgagcgca tacgacaggt
    19021 tatagcgcgt gtcctcatct ttaggattga gccgcagagc atttttataa gcctctatgg
    19081 cttcattata tttttttgat tttagaaatg catttcctat attatgccaa acatgagccg
    19141 aattctggga tgaaaggtca ctacgattgg caagactgct ccatgctttt actgattcct
    19201 cgagattgtt ctgcttataa agtgaattgc caagattata ctgagatacg gcagaagaag
    19261 gatttttttc aagagcctta cgaaaggcta tttcggcttc attatatttg ttcgactcgt
    19321 actcttttgt tcctttacgt ataaaacgat tctcccgctg cgcttcaaca gtgctgaagt
    19381 gaattaataa taaaactaac aaacataaat atcttttttc catcactata aatagtttat
    19441 tgtccaaaaa gatttatccg ttttgcccaa tggctttttc gtgctggaat caacatttcc
    19501 agaactaaaa gcacaaacgc aatccataaa aagtaaggat accagctttc atattccgaa
    19561 aataactgag tatcgaattc ggttttttcg agcttgttaa tttcctcgaa aatttttctt
    19621 aaggcttgat tggtattttg tgccctgata taaatgcctt gtccggtcga tgctactttt
    19681 tgaagtgtaa tttcatcgag ccgtgtaata atcgtattgc cctgagcatc tttcttgtac
    19741 ccagttattt ctcctttttc attgacgatt gggataggtg aaccttcagg tgtaccaatt
    19801 ccaatggtgt aaatacgaat gccttcagca gctgcttttt cggcttctgt caaaacatct
    19861 ccctcatggt cttcgccgtc agtaatcaat ataatggctt tgctacgtgt ttctgattgt
    19921 aatgaagcct ttgccatgct aatggcttga gcaagagccg ttccttgagt aggaatcagt
    19981 cgtgtgttga tgttgttgat aaacatttct gcggcagcat aatcactagt gatgggcaac
    20041 tgaatataag ccttgcctgc aaagacaata atacctatcc tatcgccctg caattgttcg
    20101 actaatttgc gtatggccag tttggcattc tccagccgat taggttttac gtcttcggca
    20161 agcatactgt tcgaaacatc caagcaaatc atcatgtcga tgcccttctg tttggctttt
    20221 acaagttttg ttcctatttg agggttggcc atagctatgg tcaatataat catggctata
    20281 ttccataaaa taagttttaa aataggacgc acattcgaat aatcaggcat caaccggctc
    20341 aaaaggcttt tctctccaaa ccgcgctagc atcttttcgc gtatgattct tcctaccaca
    20401 aaaagagcca ccgaaattgg aattatcaat aaaaaataaa aatattctgg attttcaaat
    20461 ctgaacatat tattcaaaat tttaaaaata actttaagga taaaccctta aaaaagtatg
    20521 cgacaagatg aattcaatga tgaggaggat tatagccact aaaacgaagg ggcgaaattc
    20581 atcggttttt ttatgaaatt ccgaaacatc tattttcgat ttttccatct ggtcgatttc
    20641 atgataaatt tcacgcaaag tagcattgtt agtagctcta aagtattttc ctcctgtcat
    20701 ttgagctatt tgctgcaaaa gattttcatc tattttaacc tctacatttt gatattgaat
    20761 cccatagggt gtttggaaag gataaggagc cataccacgg cttccgacgc caatggtgta
    20821 aatgcgaatt ccatattttt ttgcgatttc agcagccgat ataggatcga tatttccacg
    20881 gttgttttca ccgtcggtaa gcaaaataat tactttgctc actgtagtgc tttcacgcag
    20941 gcgattgata gctgtagcca atccatcgcc aatagctgtg ccatcttcta tcattccact
    21001 ctttaattca cgaaaaagtt ccaataaaac agttttgtcg gttgttagcg ggcactgggt
    21061 aaacgcttct cccgaaaatg caaccagtcc tattctgtca ttaggcctac cttccacaaa
    21121 ttctgaagct acaatttttg ctgcttcgag gcggttgggc gagaagtctt cggcaagcat
    21181 actgctcgaa atatcgagtg ctatggtaat atcaattcct tctatgctta catcttgtcg
    21241 gctaatactg ctctgcggcc ttgctaaggc aattatgatg aaaataagag ataaaattcg
    21301 taaaataaag ggaatatgac gaaagcgctg tctgggtgta tgaggtagct tgctaaaata
    21361 acctgtttga ggaaatgata atgtaggatg aagctttttc cctctgtaaa tgtaccagcc
    21421 cacaagtagg ggaatagtca ataataagag taacaaccac ggatgcgcaa aagtgatatt
    21481 atgaatgaat ccaatcatag tggtttattg ttgcatttca gatggagaag aaggatgggg
    21541 ttccgaagca ttttcaggtt tgggcatggt ttcttccaca aaccttcgac aaacatcaaa
    21601 tgaaaatgaa ttctcatcgg gtaaaggtat gtaacgtgca aattttacca gatcagcggt
    21661 ttgcaacatt cttcggcaat cactgatcaa tttcgtatca aagcccgcat tttctaaagc
    21721 tatgataatt tcttcgctaa ccatttcggg agcatttatt tgaaattgat tgtccagata
    21781 atccctaaag atatctgtaa gttcagaata gaattgctta taatgacctg actgccataa
    21841 ttgtttgttt ctgagcgtat ctaatgcttc cagcgcaatt tgccaagccg gaattttggg
    21901 cttaggagca atgaggaaat attttcgctt gcctcttcgg tactgaacat agcggtaaat
    21961 tagaaatgcc acaatagcaa tcaagagggc ataccctatc catggcaaga tttctttcca
    22021 actatatccg attttctgta tgcctttaat gtcgcggatg tcttgagtcg tatcgactgg
    22081 catagccatt acctccagca aaattggttc ggtttcggct atttgcgtaa ttgtgtcctg
    22141 gggaagttgc cagtagaatc taatgggttg tactacaatg tatcccgtat caaaagatgt
    22201 aatgacaaga ttctggcttc ttttaaccat tccatcgggt gtaactgtcg aatcaatagc
    22261 agatttttta ataatttcga tttggcgact tagtgtatct cctctaattc cccatcctat
    22321 cttagcctta ggtggatggg taaaactgat attcattctc acctgttggc caatcatcaa
    22381 gcgagttgaa tcaatttgga cagttgcttt ggcctcgcct gtttgagctc ttaattgtat
    22441 cccagcaagc aagagcgata atgttagaat aattatttcc ttgaaaggta tttttttgtt
    22501 catctcaaca agttggttgc tgaatataac gactgaattt tttttgacat ctccaatgtc
    22561 tttttctgga ttagtttctt aaaagatttg atggactttt actttcattt atttccttct
    22621 ttctcttatt tggaaaaggc gaataagagg agggacataa tcttgagcag tatgtatgct
    22681 aatgttgtct attccgcagc gggtaaacac atctcgcaga tagcgctggt ggtgtttgcg
    22741 catattggcg tacctttctc taatctgaga attggaggtg tctatccatg taatttcccc
    22801 cgtttcaggg tctttagctt caaccaagcc aatattgggc agttggtctt ctctttcatc
    22861 atacacccgc agagccacaa cgtcgtgctt gcgtgaggct acttccaatg ctttatcaaa
    22921 tccttggtct tcaaaatcgg agattaaaaa tgcaatgctg cgttttttaa tagcattggt
    22981 aaaaaacctc aaagcttcgg ctatattggt attagaatgt ttgggtttaa aatctatcaa
    23041 ttcccgtata atgcgcaata tatgcgtggt tcctttcttg ggtggaataa atttttcaat
    23101 accctggctg aagaatatca cccctacctt atcgttgtta ctgatagccg aaaaagctag
    23161 gactgcagca atctctgtca taagttcctg tttgaacctt tgccgcgaac caaattcatt
    23221 tgaaccactt acatcgatga gcaatatcac agtaagttct ctttcttctt cataaacctt
    23281 tacgaaaggg tgattcaatc gggcggtaac attccaatcg atattacgga tatcatcgcc
    23341 atattgatat tcacgaactt cgctaaattc cattcctttc cccttgaatg aactatgata
    23401 ttctccagag aaaatctgat tggaaaggcc acggcttttt atttctattt ttctgacttt
    23461 tttgaagatt tcagaagtat ccatcgtaaa aaaattaaaa tgtgataaga aaatgataga
    23521 ttcaattatc agggtacttc gacggtattc agtatctggc taataatttc ttcggtagtg
    23581 atgttttcgg cttcggcttc gtaggttagt ccaatgcgat gacgcatcac ctcgtgagca
    23641 acggcgcgaa catcttctgg aataacatag ccacgtcttc taatgaatgc aaaggctttt
    23701 gatgcaagag ccagattaat acttgcacgt ggggaggccc cataagagat caggcctttg
    23761 aaactatcta ttttgtaatg ttcgggaaaa cgtgtggcaa acacgatgtc agtaatgtag
    23821 ttttcgattt tctcgtccag atagacttct cttactaagc tacgtgcctt gatgatgtcc
    23881 tcgggtttga gccgtgtttc ggtgaaaacc tcctcgttgc gaatgttttg ccttaagatg
    23941 agtttttctt cttccttgtc gggataagta atcactacct ttagcatgaa acgatca
//
LOCUS       DAOORU010000016        23461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_2641_length_23461_cov_6.109160, whole genome shotgun sequence.
ACCESSION   DAOORU010000016 DAOORU010000000
VERSION     DAOORU010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23461
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2641_length_23461_cov_6.109160"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            144..557
                     /locus_tag="PLF20_02265"
     CDS             144..557
                     /locus_tag="PLF20_02265"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOX79865.1"
                     /translation="MEEFIEIISQLSHLIGEAEEKAREDYHKLHLTITQMHYLEMINF
                     MHNPNITELAAQLNLSKPSVKAVVDKLIEKSCIYKVRSDADRRSTHLHLTEKGKLIQQ
                     MHDKAHRNIAELFEHKLTEEEIGKLEELLTKVLRE"
     gene            731..3274
                     /locus_tag="PLF20_02270"
     CDS             731..3274
                     /locus_tag="PLF20_02270"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOX79866.1"
                     /translation="MITNLQEYTVRGLTHEEVERRLAMEGYNELPSSRPRNLFQIALG
                     VLKEPMFILLVVCGSLYLILGDLQEGLMLLSFVFVIMGIEFYQEKKTERALDALRNMA
                     SPRALVIREGETIRIPGREVVKDDIVVLQEGDRVPADAIVLSCVNLSADESMLTGESI
                     PVRKREWSEDDVTFSPGGDDVPLVYSGSMIVGGNAIVKVLATGIHTEIGKIGKALESV
                     KEEPTQLKREMGMLVKRLTWMGAILCVMVIVIYTLTRGNLLKGFLAGITLAMAMLPEE
                     FPVVLTVFLALGAWRISKKNVLTRQPAAIETLGSITVLCTDKTGTLTQNKMAIAQLYN
                     GKEFAVLTTDGNFTEEFHQIIEYGILASQTNPFDPMEKAILRVGEKYLQNSEHIHHDW
                     MMEKEYPLSEEMLAMSRVFSNNQTMEKVIATKGAPEAIFDLCHLSGDEIRKHQKAIEK
                     MASKGLRVIGVARSRIAHENLPPQQHDFDFEFLGLIGLMDPIRENIPQAIAQCYKAGI
                     RIIMITGDYPVTAVNIGKEIGIKDPELYLTGQDIEELTEEELSEKIRKVNIFARVVPE
                     QKLKIVNALKRNQEIVAMTGDGVNDAPALKAANIGIAMGEKGTDVAREASSLVLLDDN
                     FASIVEAIRMGRRISDNLQKALAYIFAIHVPIAGLSLIPVLFANLPLILWPVHIVFME
                     LIIDPACSIIFEAEKEEKNVMTRPPRPLDEPFFDTKKILMSCAQGIGLLLMSLLVYFI
                     GLRMGYSENAVRTFTFVTLIVSNVSVILCNRSWSSSIIKIISTPNPTVKWIIGGAIFF
                     LLLVLKIPALQDLFLFEEIGWFEIFICALAGISSIIWFEIYKQLKKEKI"
     gene            4042..6381
                     /locus_tag="PLF20_02275"
     CDS             4042..6381
                     /locus_tag="PLF20_02275"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOX79867.1"
                     /translation="MENANLKSELSNATDLTEETPVELSESPSSNNILPNDESPQILE
                     HTPHQPTEFSVEQNSNETELPLSSEVNLSLETIQPAAEIHIAAKIDETTTEVQDNGQN
                     VEIESMQVEGSGSAPEEEVAEKCISDAPQVETVSEHQTNDSANAVDNDPVKMELVEEE
                     AQDVQSVEIHSQIVEENQRVTESSMVEEEHPDMESEFLKEIDFQGVDYDLLSREKLVE
                     VLKSLVDSDDINGIKTQVGLIKVTFIRKTKERQKQELESYIASGGREDEFQYQPDDIE
                     HQFDAVFDIYKEKKLQFAEELERQKQQNLEAKLAILDELRALINSEETLKKTYDEFRN
                     LQEKWRQIGMVPKSEVNTLWQNYHFLVEKFFDKVKINKELKDLDLKKNLESKIELCEK
                     AEELLLEPNVMKSFKELQKYHEQWREIGPVPSEKRDEIWERFKAATEKINARRREYYD
                     KLQDELRNNYESKQALIEKAREIIETPAETIRQWQENTRAIDELFKIWRSVGPAPRKV
                     NDQIWNQFKELLDQFYKQKSEFFSKIKEQQLHNYNLKLDLCVQAEALRDSTDWKNTTK
                     EMINLQKEWKKVGPVPRKQSEKIWKRFRTACDQFFKAKEEYFSNIHQNEKENLRLKEE
                     LIKQVEEYPYTDDKAENLRVIKEFQRKWVEIGYVPLNEKERLQNSFRKAIDKQLDRLN
                     ISPIEVDAMSYKLRFEQIKDLPDGHKTIIREINFLQNKAAKMTEDVTLWENNLGFLAS
                     SKNADILRQEFERKIHKTKQEIKLIEAKVKFLKEELNKK"
     gene            6718..7743
                     /locus_tag="PLF20_02280"
     CDS             6718..7743
                     /locus_tag="PLF20_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOX79868.1"
                     /translation="MNKILDSFDRKSPLKWLVVIGLIVIIGFIIHAHIEKPLIDEIIG
                     DDFVKQGDMKVVPVPIPKKLSFAGEEVPLNLFYVRESLDRELTVNTYWHSQSLLLFKR
                     AHRYFPVIEPILKKNDIPEDFKYMILAESGFLHNISIAGAAGYWQFIKSTAQEYGLEV
                     NQYVDERYNLEKSTEAMCKFLKKAYEKYGTWTLAAAAFNGGMGRFDRIVQSQKTTNYY
                     EMYMNQETSRYIFRILAFKLIFEHPERYGYYFKKENLYPPIPSKEIVVKEDISNLIEF
                     AQKQGINYRILRELNPWISDTSLIVSPNKRYRIKIPTGNYKNYDEMIREEVNKEASVP
                     DNSYDKD"
     gene            7730..10594
                     /gene="uvrA"
                     /locus_tag="PLF20_02285"
     CDS             7730..10594
                     /gene="uvrA"
                     /locus_tag="PLF20_02285"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOX79869.1"
                     /translation="MTRIEEGIPKDILLQKEDNLEVYGARVHNLRNIDVIIPRDKFVV
                     ITGLSGSGKSSLAFDTIYAEGQRRYLETFSAYARQFIGNLERPDVDKIVGLSPVISIE
                     QKSSGSNPRSTVGTITEVYDFMRLLFARIAKAYSYMTGELMVKYSESNLIEIIRKEFE
                     DCKVDILAPLVRGRKGHYRELIDSLRKQGYSRIRVDGDIKSLSEIRQLDRYKTHDIEL
                     IIDRIKIGGSSEERLTSSVQTALKQGKGTLIVFNESDGSMRYLSRNLMCPTTGIAYED
                     PEPNSFSFNSIYGACPKCSGLGRISEIDLEKIFPDKNKTTRQGGIAPLAEYRDNWIFR
                     QIEGIGQIYGFHLDTPIKDLSEQALNAILYGVDGGFKVRKEYDSGVIQTYTLEFDGIV
                     DFIIRQYQESETASIRKWVGEFLNKVTCPVCNGSRLKKESLWFKVDGKNIAELAMMDL
                     GSLDHWFGQLPERLQGNDRIIGQEIIREIRSRLWFLLELGLGYLTCDRPAYSLSGGES
                     QRIHLATQIGSQLTGVLYILDEPSIGLHQRDNHRLIKALKNLRDIGNSIIVVEHDKEM
                     IVSADYVVDLGPGAGKKGGWVVASGTPQQLLQSNSLTCQYLSGKKKIEVPPKRRLGNG
                     QSLILKGARGNNLKNINLKIPLGKFICVTGVSGSGKSTLIEETLYPVISQALYRSEQK
                     PLPYDSLEGLKNIDKIIQIDQSPIGRTPRSNPATYTKVFDEIRQLFARLPESQVRGYK
                     PGRFSFNVRGGRCEACGGAGVRVIEMNFLPDVTIPCEVCQGKRYNRETLEIRYKGKSI
                     NDVLNMTIDQAVEFFANVPSILRKIQTLKQVGLGYITLGQSSTTLSGGEAQRVKLSSE
                     LSRRDTGKTLYILDEPTTGLHFEDVRVLLDVLQKLADKGNTIIVIEHNMEVIKVADHI
                     IDLGPEGGDQGGYIVAEGTPEEVAQNPKSYTGFYLKQVFS"
     gene            10602..12269
                     /locus_tag="PLF20_02290"
     CDS             10602..12269
                     /locus_tag="PLF20_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOX79870.1"
                     /translation="MMNTIEIICQNCDERREFPKGITLAEVSRQFNVKLKYPILGALV
                     NNKLRELSFDLYKPKTVNFIDFSHNIGYRMYVRSVYFLLYAAVKEVIPQALLRIDHSV
                     SKGWYAELEGENIELNEDLLKRIRERMVEMVAADLPFTRQEILTAEAAQLFMKNGQMD
                     KAELFGRRHEMYTSVYGLNGFINYFYGYLVPSTGYLTLYDIRHYYKGLLIRVPDPENF
                     EIPPAVTHQPKLFKVFREHKKWINILDVPFVGNLNKATSEKRDTDLIKISEALHEKRI
                     ARIADKIYRWYDKVKIVLISGPSSSGKTTFSKRLSIQLMVLGLKPVQISIDNYFVDRE
                     HTPRDEKGEYDFESLQAIDIDLFNENLNDLLIGREVRLPRFDFNSGRRSYYNGTFKMK
                     PDQVLIIEGIHGLNPDLTPHIDPARKFGIFVSALTQISIDAQNPISSTDNRLFRRMVR
                     DYRFRGYSALETLRRWDSVRNGEEKNIFPFQENADVMFNSALLYELAVLKTYAEPLLK
                     TIPEIEPEHAEALRLMKFLSYFQPISSREIPPTSILREFLGGSSFIY"
     gene            12279..13133
                     /locus_tag="PLF20_02295"
     CDS             12279..13133
                     /locus_tag="PLF20_02295"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HOX79871.1"
                     /translation="MKRIEIPFQIFKILEQGYHIAIAVKVNGMPAHWIIDSGASHSVF
                     DKARISCFLPEFQLKESPMMAMGLGNELEPFLLKVSDFEIEKRKISKYQATLLDLSPL
                     NQIYSRYFNDPIHGVLGCDLLVKLKAKLNYKKKTIELKKEKKPMQILSIMPDSEHLMV
                     TLKVRKQKANMLIDTGASSTIFDLNLFKQFYPYEASQLERTDQPSAGIANDAQNFGAA
                     LIPELCIGPVKMTNYEMLLIDLEHINSLYSKLDLPPIDGLLGNDLLFKLKASLSFKSH
                     SLRLPFPH"
     gene            13377..13979
                     /locus_tag="PLF20_02300"
     CDS             13377..13979
                     /locus_tag="PLF20_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79872.1"
                     /translation="MAKKKYNLRKTIRRGLKQGWTKQQVFDHLIETLEVEISNDYRWI
                     KYIAKEISFTPPFRIRNRLRVGIIFLILFVIVSCFISLMQQFPMMKIYGIIEFPLWIT
                     IIWFLGTNFFNILNLFLLGPVLRWNRQALLLLGIINAFLFIRTVFIWKLNIGLMPFIT
                     LAQCVSRFFIFFLSFIIYRFVRDNYIIKQEENGIWKVIFT"
     gene            14064..18488
                     /locus_tag="PLF20_02305"
     CDS             14064..18488
                     /locus_tag="PLF20_02305"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOX79873.1"
                     /translation="MAIMLHPGVQTALGEKVAGKLSQVLSVKVGIGGMYLHPLSGIVL
                     RDVYMEDSCGTVLIRAERLSVNINMIQPHQKILFFNSVKAENAEVNLVKYYGDKKLNI
                     THVFGKFSSGKPKHEQPRKFGVGDLKIIVRRFEFQNSHFMYQDQNKCGKREKGMDYAF
                     IEINGINLEAFSYIFTGDSMTFRVKELQAEERSGLKFEHLQGKFTVSSQHLIIDDFKF
                     KTPYSDVNMKLTFEYNDFDDYNHFVDRMYIQTEFQDSRLNFIDFSPFAVSLEGMNQNI
                     LVKGKVFGKVPDMRGEALTIRTGEKTIFKGDLQMTGMPDIEETFIVLKARQLYADMKD
                     VNNFTLPGNFRLKEKIHLPKQIYELGFVSISGEFLGFYNDFVANARFTTGVGVLITDL
                     KLMALPNGQYEYRGNLATDNFDLGRILEVPQLIGAVSMAGDVEGKGFSLDSANVRLNV
                     MVQKFEFNRYPYKNLTVRGELSKKQFQGEINIDDPNLNLNFDGSIDLSEDIPEYNFTA
                     GVSHAYLQNLGMIDRGDSLCELNFELRANFLGSHLDNLEGSIDLTEVNYTEKGKDYSA
                     NRIFLNTLLTPTGERTLSLLSPFFDASVRGEIQFAQLGYSFSLFFNNYLNSFHNLGQT
                     LSDTTGQNFVYDIRIKKLNELTALFLPWLKVANNSRIKGQFDTKDRILSLEAQTDTLS
                     VYGVKMNNLSLRSQSFQDAITLETNIRKVNFVEMKKDSILYFGLDKFDLTANIAYDSI
                     RYNMKWGLLPEDSSNYGNLKGYLSFSNYPEIFMQFIESSFNLDDNIYVIQPDNKIIFR
                     KQEIDIHRLEITSENESLYIDGAYSSDSTKMLNAQFHNFSLSHADFFLQPFGLQIGGL
                     LQGHIQLHQEDNKPLIQAEMRIDHLAVNQQNLGFMELTSLWNTKSKALFVKAKSTLVN
                     NKFQSHPIDLSGYYYPSDPKQNFDIKLKMENFNLKPIQPFLNIAIAELEGFLSSNIEL
                     EGSVKSPEIHGKLKFIRAKIRPKFFNTTYSFSDEIIVEPSRIIFNKLKINDSLGNVAF
                     CDGYIAHKNFQDMYLDIRLQPENFILFNAEPQQNKYVNGIAFISGQIRVSGSINNLNI
                     EANAITEKGSNIYVPLSSTVDVRSSSFLYFKQPEDTLKLKKVERTNLNVSGFNVFSVL
                     TLTPQARIKVSLPQDAGTIEGTGEGMITLEVNSRGDLNINGQYEIQSGSFLFNLQNIL
                     SRMLEIEKGSTIHFSGDPLDAQINLRASFTTRTTLSGLGLDLDSTVTSARIPVKTIIH
                     LQNKLLNPFITFSIQFPKIDPDIERAVYAKLDTTNQMVMTQQFVSLLVLNNFSFAVTN
                     KSFGNSLGISSFQMLSNQISNLLSQISQDFDIGINYRPGDAISAQELELALSTQLFDQ
                     RVTIDGNLGVVGMQNANKTSNVVGDINVEVMLTRDGKLRLRAFNRSNTTELLTVSAPY
                     TQGIGLFYRKDFNNLAELFRKKRKIIQEESLNISTSAN"
     gene            complement(18485..19498)
                     /gene="holA"
                     /locus_tag="PLF20_02310"
     CDS             complement(18485..19498)
                     /gene="holA"
                     /locus_tag="PLF20_02310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOX79874.1"
                     /translation="MTFEQIYTDLKNKIYKPLYLFYGDESFYIDFLTDYMTGNILKEE
                     EKDFNLSVFYGLNTTGSSIVDRARRYPTMADYNLVIVREAQQLRSELDQLSQYALHPS
                     SSTILTVALKLQSSRKHELLKDFKKAVDKFGVSFESKKLYDDKIPQWIRKYLSARGYT
                     ITDHACQMMADDLGNDLSLIVNECAKLIINLPKGSNITPEIIEKYIGISKDFNPFELC
                     RTISQRNKFGTAQIVLYFASNPKENPPIKISTVLFNYFQKLWIYHQLNDKKASVAAPI
                     LGVNPFFVKEYAAAARNFNLPQCSQVLQLISEYDLKFKGVDIDQADPVEMMKEMIYKI
                     LSV"
     gene            complement(19523..20302)
                     /locus_tag="PLF20_02315"
     CDS             complement(19523..20302)
                     /locus_tag="PLF20_02315"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOX79875.1"
                     /translation="MKTKEEIVKNWLPRYTGVPLEDFGKYILLTNFMDYLNLFSSIMG
                     VKVRGLDRTMPVACAGDISMINFRMGSANAATIMDLLSSIQPKAVLFLGKCGGLKKKN
                     NIGDLILPIAALRGEGTSNDYMPEMVPALPAFALQKAISTTIRDYGKDYWTGTVYTTN
                     RRVWEHDEEFKFFLRKTRCMAIDMETATLFVTGFANHISTGALLLVSDQPMIPDGVKT
                     EEKDREVSLHFTELHLRIGIDSLRQLINNGLTVKHLIFDLE"
     gene            20663..21088
                     /locus_tag="PLF20_02320"
     CDS             20663..21088
                     /locus_tag="PLF20_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HOX79876.1"
                     /translation="MKIMTRTIDGREEIFDEFRGRYVELTPEERVRQWFAHFLVEDCA
                     YPRGRIGIEISIVIQRKTYRSDLVVFDKNRRPWMLVECKSPEIALTEKVFEQVTLYNY
                     TLKADFIVLTNGNSTIIMKLDYAAGKIEFQSELPPYPKD"
     gene            complement(21133..21264)
                     /locus_tag="PLF20_02325"
     CDS             complement(21133..21264)
                     /locus_tag="PLF20_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79877.1"
                     /translation="MNKELIAYKFTGSASGTYKKQASVSVLVDYAARLRRAENFSKL"
     gene            complement(21257..21919)
                     /locus_tag="PLF20_02330"
     CDS             complement(21257..21919)
                     /locus_tag="PLF20_02330"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOX79878.1"
                     /translation="MKNKRVVLAALLMITMVAFSVQTKAQKGYSFNVNAMVQNSWLFN
                     SDDSDMDNFSQKAYIHPALSIGAGYNFTEKLGMGLDVMYSFQGRKQEVGGVETRIKLD
                     YVKIPLYFHFNTNPAQKWMFSLNVGPQFNILTTAKYLDANDNEMDIKHHYENLTFGGF
                     IGAGGEYQINSKFNVFGMVRFDYDFTNPENKDALGYNPDRANSHNSTLGLQVGLRYKL
                     GE"
     gene            complement(22253..22669)
                     /locus_tag="PLF20_02335"
     CDS             complement(22253..22669)
                     /locus_tag="PLF20_02335"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOX79879.1"
                     /translation="MAKLGIVSSPSEIPFEIKPDVLGDVVYDLNFFVERPPTEDKKIT
                     ETPKDTLKVTEIRIPLVQEFYTLQTGAFKHKHNAEQLSETLKRTFELPVWIKYENKLY
                     KVRIGRFSDYIEAKNFRSMLREKGFDAFIIEKKSNN"
     gene            complement(22768..>23461)
                     /locus_tag="PLF20_02340"
     CDS             complement(22768..>23461)
                     /locus_tag="PLF20_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79880.1"
                     /translation="PQWKGFTSRVQSQINFSDNRVISLRAEIEKRILRQGYLSLMGEE
                     NFQSKYRAISLSFRWTVPFSQVNLATRLSNNEFMITEGAQGSFAFGSGKGYVHAEDRP
                     SVGMGGLSIISFIDLNHNYKRDSGERLVKGLAMRINGGRVIQGEKDSIIRVVGLIPYT
                     DYILTIDEKSLEEISYRIAHKVIRIYAEPNQFKKIEIPVLPMGEINGWVLRKDGTGQR
                     GEGHIIIDIIRP"
BASE COUNT         7158 a         4446 c         4867 g         6990 t
ORIGIN      
        1 tgtgagtaat atgcctgaaa taaacccaga ccagaaaagc agtcattatg ttttttgatt
       61 agctaaatga ctgattcgat aaaaaatttc aattgaaact tatttttaaa aattaattag
      121 taaaaactaa ctatttttgt tgcatggaag agttcatcga aatcatatcg caattatcac
      181 atttgattgg agaagccgag gaaaaggcac gcgaagatta tcacaaactt catctcacca
      241 taacgcagat gcattatctg gagatgatca actttatgca taatcccaat attactgaac
      301 ttgctgccca gttgaaccta tcgaaaccca gtgtgaaagc tgtagtggat aagctcatcg
      361 aaaagagttg catttataag gtaaggtctg atgccgacag gcgtagcaca catttgcatc
      421 tcaccgaaaa aggaaaattg attcaacaaa tgcacgacaa ggctcacaga aacatagctg
      481 aattatttga gcataaactc acagaggagg aaattgggaa actggaagag cttttgacga
      541 aggttttgag agaatgaaac gaaatccttg ataaggctga gactacaaga gtatacaaat
      601 catctggaag gaaaattgtt aattgggatt tttcattttg attgcttttt agaatgaata
      661 aatgagatat aagatggttt cattggtttc gaaaaaatag ttagcaaatc ctaacaagat
      721 aaaaaagaaa atgataacga atcttcaaga atatacagta aggggtttaa ctcatgaaga
      781 ggtagagcgg aggcttgcca tggaagggta caatgagctg ccatcatcaa ggccgaggaa
      841 tttatttcag attgcgctgg gagtactcaa agagcctatg tttattctac tggtcgtgtg
      901 tggtagcttg tatctgatat tgggggatct tcaggaaggc ctgatgctgc tgagttttgt
      961 ctttgtcatc atggggatag aattttatca ggaaaagaag acggagagag cactcgatgc
     1021 attgagaaat atggcaagtc cacgagcatt ggtaatacgt gaaggtgaaa ccataaggat
     1081 tcccgggcgc gaagtagtga aggatgatat tgtagtttta caggaaggcg atcgtgttcc
     1141 ggccgatgct atcgttttga gctgtgtgaa tctatcggcc gatgagtcga tgctaacggg
     1201 tgaatctatt cccgtgagga agagagaatg gagcgaagat gatgtgacct ttagccctgg
     1261 aggcgatgat gttcccctgg tttattctgg ttcgatgata gttggaggga atgccatcgt
     1321 taaagttttg gctactggaa tacacacaga gatcggtaaa attggcaagg cgctggagtc
     1381 ggtgaaagaa gagcccacac aattgaaaag ggaaatggga atgctggtaa aacggcttac
     1441 ctggatgggg gctattctgt gtgtaatggt gatagtcatt tataccctca caaggggcaa
     1501 tttgctgaaa ggttttttgg cgggtattac acttgccatg gctatgctgc ctgaggaatt
     1561 tcccgttgta ttgacggttt ttctggcgtt gggagcttgg cgaatctcta aaaaaaatgt
     1621 gttgactcgg cagccggctg ccatcgagac tttaggctcc attaccgtgc tttgtactga
     1681 caaaactggt actctcactc agaataaaat ggccattgcc cagctttata atgggaaaga
     1741 atttgctgtt ttaactaccg acgggaattt tacggaagag tttcaccaga taatagaata
     1801 tggaattctg gccagccaaa cgaatccttt cgatccaatg gaaaaagcca ttctgagggt
     1861 aggggaaaaa tatttacaaa attcggagca catccatcac gactggatga tggagaagga
     1921 atatcccttg tcggaggaga tgttggccat gtcgagggtt ttttctaata accaaaccat
     1981 ggaaaaagtt attgccacca agggtgctcc cgaagctatt tttgatttat gccatttgtc
     2041 gggtgatgaa ataaggaaac accaaaaagc catcgaaaaa atggcatcga agggactgcg
     2101 tgtgattggc gtggctcgct cgagaatcgc tcacgaaaat ctgcctcctc aacaacatga
     2161 tttcgatttc gaatttttag gtttgattgg tttgatggac cctattcgtg agaatattcc
     2221 tcaagccata gcccaatgtt acaaggctgg aataagaatc atcatgatta ctggagacta
     2281 tcctgttacg gctgtcaata taggaaagga aattggaata aaggatcctg aactttatct
     2341 gactggccag gatattgaag agctgacaga agaagaactc agtgaaaaaa ttaggaaggt
     2401 taacatattt gcgcgtgttg tgcccgaaca aaaattaaag attgtaaatg ctctgaagag
     2461 gaatcaggag atagtggcca tgacgggtga tggggtaaat gatgcaccag ctttgaaagc
     2521 cgcaaatatt gggattgcaa tgggtgaaaa aggtacagat gtggctcgtg aagcctcttc
     2581 tttggttttg ctcgacgata actttgcctc tattgtggaa gccataagga tgggacggag
     2641 aatttccgat aatctgcaaa aagcgcttgc ctatattttc gccattcatg tacccattgc
     2701 gggtttgtcg cttatcccag tgctatttgc caatttgcct cttattcttt ggcctgttca
     2761 tattgttttt atggaattga tcatcgaccc tgcctgttcc atcatttttg aagccgaaaa
     2821 agaggaaaaa aatgtaatga cgcgtcctcc ccgaccattg gatgaaccct ttttcgatac
     2881 aaaaaaaata ttgatgagtt gcgctcaagg tattggattg ctccttatga gcctgctggt
     2941 gtattttata ggcctaagga tggggtattc ggaaaatgct gtaagaacct tcacctttgt
     3001 aacactcatc gtttcgaatg tttctgttat tctttgcaat cgttcgtggt cttcctccat
     3061 aatcaaaata atttcaacac ccaatccaac cgtaaagtgg atcataggtg gtgccatctt
     3121 tttcttactt cttgtattga aaattcctgc tttacaagat ttattcctct tcgaggaaat
     3181 tggctggttc gaaattttta tttgcgcttt ggccggaatt tcgagcatta tttggttcga
     3241 aatctataaa caattgaaga aggagaaaat ataaagcaaa gaaaacgaag gtatcgattg
     3301 atctcgctcc tcaaagttga agcgagctta tcgtgctttt gttcatcgac aattttaatt
     3361 gaaatggatt gaaaattgca aggatgggtt tttaaaaact atccctaaag ttagttgatt
     3421 ttacccattt atgtcaatcg ttatggtttt ttatattcat cttcgacttc tgggttcatg
     3481 agccgaggtt aatgtttcgt ttttttgacc aacaggatta cttataagat attttctttt
     3541 attttctcta taaatttcct aaagtttttt tctgaagggg cttataatag acatttatta
     3601 aatatttaga acaaagtaca acatagcgga agagataaga ctttcgcatt aaaaatatta
     3661 atttcctctt cgaactcaaa gtttcttcac ggggttcaat tcggagagtt tgctatagtc
     3721 aatttgcgaa aacgggatat tgatccacca caaatttaaa gactttacaa accatccgac
     3781 acattcctta ttctaccttt attgaggtaa tagaaggaat gtttttcttt aaatgatatt
     3841 catttattac ttatagaaca atcatggcct tgatttttgt tgtgcggcgt atgaaaatta
     3901 ttttttttat gtaagtttgc agcccatatt cgtaaaatgc aaaccctact aattatcata
     3961 ccgtcagttt aaacatccca ccgcgcctgc ggtatcctat cttcgaaatt atttagtaat
     4021 cacttcctta ttctattcac tatggaaaac gcaaatctca agtcagaact ctcaaatgca
     4081 accgacctca ccgaggagac tcctgttgaa ttaagtgaat cgccatcttc taataatata
     4141 ctaccgaacg atgaatctcc ccagattctg gaacatacac cccatcaacc gactgaattt
     4201 tctgtcgagc aaaactcgaa tgaaacagaa cttccactat cttcagaggt gaatttatca
     4261 ttagaaacga ttcaaccggc tgctgaaatc catattgctg caaaaataga tgaaaccact
     4321 accgaagttc aagataatgg ccagaatgtc gaaatagagt cgatgcaggt ggaaggcagt
     4381 ggatcagctc ctgaagagga agtagctgaa aagtgtattt cggatgctcc acaggtcgaa
     4441 actgtaagtg agcatcaaac taatgattcg gcaaacgctg tggataatga cccggttaag
     4501 atggagctgg tcgaagaaga ggctcaggat gttcaatctg ttgaaattca ttctcaaatt
     4561 gtagaagaaa atcagagagt tacagaaagc tcaatggtag aagaagagca tcccgatatg
     4621 gaatctgaat ttttgaaaga aatagatttt cagggagttg attatgatct gctttcgaga
     4681 gaaaaacttg tggaagtgct caaatcgctt gtagatagtg atgatattaa tggtatcaag
     4741 acccaggtag ggcttattaa ggttactttc atacgtaaaa caaaggaacg tcagaagcag
     4801 gaattagaat catatatagc ttcaggaggg agagaagatg aatttcaata tcagcccgat
     4861 gatattgaac atcaatttga tgccgttttc gatatttaca aggaaaagaa gcttcagttt
     4921 gcggaggagc tggaaaggca gaagcagcag aatttggaag ccaaactggc cattctggat
     4981 gaattgaggg ctttgattaa ttctgaagaa acactgaaga agacttatga tgaattcaga
     5041 aatcttcagg aaaaatggcg tcaaatagga atggttccca agtcggaagt taataccctt
     5101 tggcagaatt atcatttttt agttgagaaa tttttcgaca aggtaaaaat caacaaagaa
     5161 ttaaaagacc ttgatcttaa gaagaatttg gaaagcaaga tagaactttg cgaaaaggcg
     5221 gaagagcttt tactggagcc taatgtaatg aagtcgttca aggagcttca gaaatatcac
     5281 gagcagtggc gtgagattgg ccctgttcca agtgaaaaaa gagatgagat atgggaaaga
     5341 ttcaaggcag ctaccgaaaa aatcaatgcc cgacgaagag aatattacga taaactacag
     5401 gatgaacttc gcaataacta tgaatcgaaa caggccttga tcgaaaaagc cagggagatt
     5461 atcgaaacac ctgcggagac tatccgtcaa tggcaggaaa atacacgtgc catcgatgaa
     5521 ttattcaaga tatggcgtag cgttggccct gcacctcgaa aagtgaacga tcaaatctgg
     5581 aaccagttca aagagcttct cgaccagttt tataaacaaa aaagtgagtt ctttagcaag
     5641 ataaaagaac aacaattgca caattacaac ctgaagctcg atttatgtgt tcaagcagag
     5701 gctttaaggg atagcaccga ttggaaaaat acgaccaagg aaatgatcaa tctgcagaaa
     5761 gagtggaaaa aggttggacc ggttcctcgc aagcaatctg aaaaaatctg gaaacgcttc
     5821 cgtaccgcct gcgaccaatt ctttaaggct aaggaagaat atttttccaa cattcatcaa
     5881 aatgaaaaag aaaaccttcg tctgaaagaa gaactcatca aacaggtgga agaatatcct
     5941 tataccgacg ataaagccga aaatttacgg gtaatcaaag aatttcaacg taaatgggtc
     6001 gagataggat atgtacccct gaatgaaaaa gaacgcctgc aaaatagctt cagaaaagca
     6061 atcgataaac aactcgaccg cctcaatatc agccccatcg aggtagatgc catgtcgtac
     6121 aaactgcgat tcgaacagat taaagacttg cccgatggac ataaaaccat catcagagaa
     6181 attaacttcc tgcaaaacaa agcagcaaaa atgaccgaag atgttaccct ctgggaaaac
     6241 aatctgggct ttttggcgag ttcgaaaaat gccgatatcc tccgtcagga attcgaacgc
     6301 aaaattcata aaaccaaaca ggaaatcaaa cttatcgagg caaaggtgaa atttctgaaa
     6361 gaggaactga ataagaaata aatcagttta gcaatttgtt ttcttgttgt gctttcaact
     6421 gaagtgcaaa agagaaaagt cctaaaaatt atggaaaaag atgctcagct ctttattcgg
     6481 tgaatgatct gttcatcagc gcaggttcat atttgagctt tgaattcaaa gccatttcgg
     6541 catgcggtaa actctggcct gaaatttgag ttttgtaacc tgctttcatc actggtttta
     6601 gtattcataa tgaactcctg aaatgggaga taacttggtt acttatcata aaaggagata
     6661 ttgtataact ttgcaagctt tcgtaaaaac aattttggtt tactttgatt cttgataatg
     6721 aataagatac ttgattcttt tgataggaaa tcgcctttaa aatggcttgt ggttataggt
     6781 ttgatcgtga taataggatt tatcatacat gcccatatag aaaagcctct aatcgatgag
     6841 atcattgggg atgattttgt aaaacaaggt gacatgaaag tcgtacctgt cccaattccg
     6901 aaaaaacttt catttgctgg ggaagaagtt cctctgaatt tgttctatgt tagagaaagc
     6961 ctcgacagag agctcacggt gaatacatac tggcattcgc agtcattatt gttgtttaaa
     7021 agagcccatc gttatttccc tgtaatcgaa cctattctaa agaagaacga tattcctgaa
     7081 gacttcaaat acatgatact ggcggagagt ggttttcttc ataatatttc gatagctggt
     7141 gctgctggct attggcaatt cataaaatcc actgctcagg aatatggcct tgaagtcaac
     7201 cagtacgtag atgaaagata taatctggag aaatctaccg aagccatgtg caagttttta
     7261 aaaaaggcct acgaaaaata tggaacatgg actttagctg cggctgcctt caatggaggc
     7321 atggggcgtt tcgaccgtat tgtacagtcg cagaaaacta cgaattatta cgaaatgtat
     7381 atgaatcagg aaacttcgag gtatattttt cggattctgg cttttaagct tatttttgaa
     7441 catcccgagc ggtatggtta ttattttaag aaggaaaacc tttatccgcc aattccaagc
     7501 aaagaaattg tagtaaagga agatatctcc aatcttattg aatttgctca aaaacaaggc
     7561 attaattatc gcattttgcg tgaattgaat ccatggatat cagacaccag tttgattgtt
     7621 tcaccgaata agagatatag aattaagatt cctacaggga attataaaaa ttacgatgaa
     7681 atgatcaggg aagaggtcaa taaagaagca tctgtccccg ataatagtta tgacaaggat
     7741 tgaagaaggt ataccgaaag acatattact gcagaaagag gacaatctgg aggtttatgg
     7801 tgcccgtgtg cataatttgc gtaatatcga cgttattatt ccccgagata aatttgtggt
     7861 gattaccggt ttgagcggca gtggaaaatc ttctcttgct tttgatacca tctatgcaga
     7921 agggcaaaga agatacctcg agacattttc ggcttacgcc cgtcaattca ttggcaatct
     7981 cgaacgcccc gacgtggata agattgtggg cttaagtccg gtgatttcga tcgaacagaa
     8041 gagttcgggt tctaatccac gctctaccgt cggaaccatt accgaggttt atgatttcat
     8101 gcgtctgtta tttgctcgca ttgcaaaggc ttattcctat atgacgggcg aactcatggt
     8161 caaatacagc gaaagcaacc ttatagaaat tattcggaaa gagtttgaag attgcaaggt
     8221 agatattctt gctcccttag ttagagggcg taagggtcat taccgcgaac tcatcgattc
     8281 gttacgcaaa cagggatatt cgagaattag ggtagatggc gatataaaat ctttatcgga
     8341 aattcgtcag ttagaccgtt acaaaacgca tgatatcgag ctcatcatcg ataggataaa
     8401 aattggtgga agctcggaag aacgtctgac ctcctcagtt caaactgcat tgaaacaagg
     8461 caagggaacc cttatcgttt tcaatgaatc tgatggtagt atgcgctatc tcagccgcaa
     8521 tctgatgtgt ccgaccactg gaattgctta cgaagacccc gaaccgaatt ctttctcgtt
     8581 taattctatt tatggagcat gcccaaagtg tagcgggctt ggccgtatca gcgagatcga
     8641 tctggaaaag attttccccg acaagaataa aaccacacgt cagggtggaa tagccccact
     8701 ggccgaatat agagataatt ggattttcag gcagatagaa ggaattggcc agatctatgg
     8761 ttttcatctc gatactccta tcaaagacct ttctgaacag gctttaaatg ctatcctcta
     8821 tggagtcgac ggaggcttca aggtcagaaa ggaatacgat agtggagtta tacagactta
     8881 tacccttgag ttcgatggta tagtcgattt tattattcga cagtatcagg aatcggaaac
     8941 tgcctcgatt cgtaagtggg taggagaatt cctgaataag gtgacctgcc cggtgtgcaa
     9001 tggaagccga ttgaagaagg aatcattatg gttcaaggta gatggtaaaa atattgccga
     9061 attggccatg atggacctcg gaagtctgga tcattggttc ggacaattac ccgaaaggct
     9121 tcaggggaat gatcgcatca taggtcagga aattataaga gagataaggt cgcggttgtg
     9181 gtttcttctg gaacttggct taggctatct tacctgcgac agacctgcct atagtctatc
     9241 gggtggtgaa agccagcgca ttcatctggc tactcaaatt ggaagccagc ttacgggtgt
     9301 tttatacata cttgacgaac ctagcatagg tttgcaccag agagataatc atcgcctcat
     9361 caaagccttg aagaatttgc gcgatattgg caattccatt attgtagtcg aacatgataa
     9421 ggagatgata gtatcggccg attatgtggt agacctcggt ccgggagctg gaaagaaggg
     9481 aggatgggtg gtggcaagtg gtactccaca gcaattgctc caaagtaatt cacttacctg
     9541 ccaatacctc agtggaaaga aaaaaataga ggttccccct aagcggcggc ttggtaatgg
     9601 gcaaagcctc attcttaagg gcgctagagg aaataatctg aaaaatatta atttgaagat
     9661 tcctcttgga aaatttattt gcgttactgg tgtttcgggt agtggaaaat ctacccttat
     9721 cgaagaaact ctttatccgg ttatcagtca ggctctctat cgttccgagc aaaaacctct
     9781 accatacgat agtctcgaag gtttgaaaaa catcgacaag atcattcaga tcgatcaatc
     9841 acctataggt cgaactcccc ggagcaatcc tgccacgtac accaaggttt ttgacgagat
     9901 acgccagctt tttgcacgct tgcctgaatc acaagtgaga ggttacaagc ccggaaggtt
     9961 ttcgttcaac gtgaggggag gacgatgcga agcctgtggt ggtgcaggtg taagagttat
    10021 agaaatgaat tttttacctg atgttacgat tccctgtgaa gtatgccaag ggaaacgata
    10081 caatcgcgaa accctcgaaa tcagatacaa gggaaaatct atcaacgatg tccttaatat
    10141 gacgatcgat caggccgttg aattttttgc caatgttcct tccattcttc gcaaaataca
    10201 aacattgaag caagtgggac tgggttatat tactcttggg cagtcgtcga ctactctttc
    10261 gggtggggaa gcacaacgag tgaagctatc ttccgaactt tcgcgtcgcg atactgggaa
    10321 aaccctttat atcctcgatg aacctactac aggacttcat ttcgaagatg taagagtttt
    10381 actggatgtt cttcagaaat tagccgacaa aggcaatacc attatcgtga tcgaacataa
    10441 catggaagtt atcaaagtag ccgaccatat tatcgattta ggtcctgaag gaggagatca
    10501 aggaggatat atcgtggccg aaggtacacc cgaagaggta gctcaaaatc ccaaaagtta
    10561 caccggtttt tatctaaaac aagttttttc ttaatctatc tatcatgaac actatcgaga
    10621 ttatttgtca gaattgcgac gaaagaagag agtttcccaa agggattaca ctggctgaag
    10681 tgtctcgcca gtttaacgta aagctgaagt atccgatttt gggggcactg gtcaataata
    10741 aattgcgtga actcagcttc gatctttata aacccaaaac agtaaatttt attgattttt
    10801 cgcataatat tggttaccgc atgtatgttc gttcagttta cttcctgttg tatgctgcgg
    10861 taaaggaagt tattcctcaa gccttgttgc gtatcgatca tagcgtgagc aaaggttggt
    10921 atgccgagct ggaaggagaa aacatagaac tgaatgaaga tttactaaaa cggataagag
    10981 aaaggatggt cgaaatggta gcggccgatt taccttttac ccgccaggag atacttacag
    11041 ccgaagccgc ccaactcttc atgaagaatg gccagatgga taaggccgaa ctctttggaa
    11101 gacgacacga aatgtacacc tcggtatatg gattgaatgg ttttatcaat tatttctatg
    11161 gttatctggt gccttcgacc gggtatctta ctctttatga tattcgccat tactataagg
    11221 gtttgctgat acgcgtgccc gatcccgaaa atttcgaaat tcctccggca gttactcatc
    11281 aacccaaact tttcaaggta tttcgtgaac ataagaaatg gataaatatc ctcgacgttc
    11341 cctttgttgg caatttaaac aaagctactt ccgaaaaacg tgataccgat ttgattaaaa
    11401 tttcggaagc cctgcacgaa aaacgtatag cacgcatagc cgataaaatc tatagatggt
    11461 acgataaagt aaagattgtt ctcatttctg gtccttcttc atcgggaaaa actactttca
    11521 gcaagaggct gtccattcaa ttaatggtgc ttggcttaaa accagttcaa atttctatcg
    11581 acaattattt tgtcgataga gagcacactc caagggacga aaaaggtgaa tatgatttcg
    11641 aaagtctcca ggcaatagac atcgatcttt ttaacgaaaa cctcaatgat ttactcattg
    11701 gacgcgaagt gcgccttcct cgtttcgatt tcaatagcgg taggcgttcc tattataatg
    11761 gaacctttaa aatgaagccc gatcaggtgc tgattataga aggcattcat ggactgaacc
    11821 ctgatttgac tccccacata gatccagcac gtaaattcgg gattttcgtt tcggctctta
    11881 cccaaatttc tattgatgct caaaatccaa tctcatctac cgataatcgt ctttttcgca
    11941 ggatggtacg cgattatcgc ttcagaggct attctgccct cgaaacccta agacgttggg
    12001 atagcgttag gaatggagaa gaaaaaaata tcttcccttt tcaagaaaat gccgatgtga
    12061 tgttcaattc ggcactcctt tatgaactgg ccgttttgaa aacttatgcc gagccacttc
    12121 tgaaaactat tcccgaaatc gaacccgaac atgccgaagc cctgagactt atgaaattcc
    12181 tttcttattt tcaaccaatt tcttcccgtg aaattcctcc tacaagcatt cttcgcgaat
    12241 tcttgggcgg cagcagcttc atctattaat cccttatcat gaaacgaatc gaaatccctt
    12301 tccagatttt taaaatcctc gaacaaggtt atcatattgc tatcgctgta aaagtaaatg
    12361 gtatgcccgc tcattggatc atcgattctg gagcctctca ttcggttttc gacaaagcga
    12421 gaatctcatg ttttttaccg gaatttcaac tgaaagagtc ccccatgatg gctatgggcc
    12481 tcggcaatga gctggaaccc tttttactga aagtttccga tttcgaaata gaaaaacgaa
    12541 aaatttcgaa atatcaggct acactcctcg atctctcacc tctcaaccaa atttattccc
    12601 ggtatttcaa tgatccaatt catggagtac ttggctgcga cctgctggtt aagctaaagg
    12661 caaaattgaa ttacaaaaag aaaactatcg aactcaaaaa agaaaaaaaa ccaatgcaaa
    12721 ttctcagcat tatgcccgac agcgaacatc tgatggttac actgaaggtt cgaaaacaaa
    12781 aggcaaatat gctaatcgat accggagctt cttcaaccat tttcgattta aaccttttca
    12841 aacagttcta tccttatgaa gccagccagc tcgagcgaac cgaccagccc tccgctggga
    12901 ttgctaatga tgctcaaaac tttggtgctg ctctgatccc tgaactttgc attggcccgg
    12961 taaaaatgac gaattacgaa atgctgctta tcgatctcga acatattaat tcattatatt
    13021 ccaaattgga cttgcctcct attgatggcc tactgggcaa tgacctgctg ttcaaattga
    13081 aagcctcact tagcttcaaa tctcattcac ttcgattgcc attccctcac tgattttctc
    13141 ccattccctc cccgcaaaat cattttgtcg agttttggct tgtctcgtaa gaattctatg
    13201 ttgaaaaact gtacgacgta aaaaacataa aaattattcg aacttatctt cggaatctaa
    13261 atattttagc tttataagct aatggaaatc attctttttt tttgaaacta cagtgttttt
    13321 ttaattgttg tgcaatcaaa agcaaaaata attgcaacat gcattatttt tgccttatgg
    13381 caaagaaaaa gtacaattta aggaaaacca tcagacgagg gttaaaacaa ggatggacaa
    13441 aacaacaagt attcgaccat ctgatcgaga ctctggaagt ggaaatctcc aatgattacc
    13501 gatggattaa gtacatagcc aaagagattt cgtttacacc tccatttcga attcgtaatc
    13561 gcctgagagt gggcatcatt ttcttgattc tctttgtgat agtcagttgt ttcatcagtc
    13621 tgatgcagca atttcccatg atgaagattt atggcattat cgaatttcct ttatggatta
    13681 caataatctg gtttttaggt acaaattttt tcaatatcct caatttattc ttacttggtc
    13741 cagtgcttag atggaacagg caggcacttt tacttcttgg catcatcaat gcttttcttt
    13801 ttattcgtac ggttttcatc tggaaattga atattggctt aatgcccttt attactcttg
    13861 ctcaatgtgt atcacgtttt tttatatttt ttctgtcatt cattatttac cgtttcgtgc
    13921 gcgataatta tatcatcaag caggaagaaa atggaatatg gaaggttatt tttacttaga
    13981 attcaaaatt ttggcttatt aagaagttaa taaaaatatt attctatttt gtcgtcggga
    14041 taacaattgt tatttcttcg ggtatggcaa tcatgttgca tcccggtgtt cagactgcct
    14101 taggggaaaa ggttgcgggt aaattgtctc aagttttgag tgttaaggtg gggattgggg
    14161 gtatgtatct gcatcctttg tcaggtattg ttttgcgtga tgtttatatg gaagattcct
    14221 gtggaactgt tttaataaga gccgagaggc tttcagtgaa tatcaatatg atacagcctc
    14281 accaaaaaat tctgtttttc aattcagtaa aagccgaaaa tgcagaagta aatctggtaa
    14341 aatattatgg ggataaaaaa ttaaatatta cccatgtgtt tggcaaattc tcttcaggaa
    14401 aaccaaagca cgagcagcct cgaaaatttg gagtaggtga tttgaagatc atcgtacgaa
    14461 gatttgaatt ccagaatagc cattttatgt atcaagacca gaataaatgc ggtaaacgtg
    14521 aaaaaggaat ggattatgcc tttattgaaa taaatggaat aaatctggaa gcattttctt
    14581 atatttttac cggagattcg atgactttta gagtcaaaga actgcaagct gaagaacgat
    14641 cgggcttaaa attcgaacac ctgcaaggaa aatttactgt atcgtcgcag catttaatca
    14701 tcgatgattt taaattcaag actccttaca gtgatgtaaa catgaaactt acctttgaat
    14761 acaatgattt cgatgattat aatcattttg tcgatcgtat gtatattcaa actgaatttc
    14821 aggattcgag gcttaatttt atagattttt cgccttttgc agtttctctg gaaggaatga
    14881 atcagaacat cttggtaaag ggtaaagttt ttgggaaagt acctgatatg cgaggcgaag
    14941 ccttaacgat aagaacaggg gaaaaaacca tttttaaggg tgatttgcaa atgacgggaa
    15001 tgcccgacat cgaagaaact tttatcgtgt taaaagctag gcaattatat gccgatatga
    15061 aagatgtcaa taattttacc ttacctggga attttcgatt gaaagaaaag attcatctgc
    15121 caaagcagat ttatgaattg ggatttgtaa gtatttcggg cgaatttttg ggattttaca
    15181 acgattttgt ggcaaatgca agatttacaa cgggagttgg tgtgttgatt actgatttga
    15241 aactaatggc acttccaaat ggtcaatatg aataccgtgg aaatttggcg acagataatt
    15301 ttgatctggg aaggatttta gaagttccgc aattaatagg ggcggtaagt atggctggag
    15361 atgtcgaggg caagggtttc agtctcgata gcgcaaatgt aagattgaat gttatggttc
    15421 aaaaattcga attcaatcgt tatccttata aaaatctgac cgtgcgtgga gaactatcca
    15481 aaaagcaatt tcagggtgaa atcaacatcg atgatcccaa cttgaatctc aatttcgatg
    15541 ggtcgatcga cttgagcgaa gatatacccg aatataactt tactgcaggg gtttcgcatg
    15601 cgtatttgca aaatcttgga atgatcgatc gcggcgattc gttatgtgaa ctaaatttcg
    15661 agctcagggc aaatttcttg ggctcacacc tcgacaatct ggaaggttct atagatttaa
    15721 ccgaagtgaa ttataccgaa aaagggaaag attattcggc caatcgcatt tttttaaata
    15781 cgttacttac tccgactggt gaacgtactt tgagcctcct ttctccattc ttcgatgcca
    15841 gtgtgagagg agaaattcaa tttgcccaat taggatattc attttcgtta ttttttaata
    15901 attatttgaa ttcatttcat aatttgggtc aaacccttag cgacaccaca ggccagaatt
    15961 tcgtctatga tataagaata aaaaagctga acgaattgac cgcactattc ctgccatggc
    16021 taaaagttgc gaataatagc cgcatcaaag gacaattcga cactaaagac cggattttaa
    16081 gcctcgaagc acaaaccgat accttgagcg tttacggcgt caagatgaat aatctttcct
    16141 tgcgcagcca atcctttcaa gatgctatta cattggaaac caatattaga aaagtaaatt
    16201 ttgtagaaat gaagaaggat agtatcttgt attttggcct cgataaattt gaccttactg
    16261 ccaatattgc atacgatagt atccgataca atatgaaatg gggcctctta cccgaagatt
    16321 catccaatta tggaaacctg aaaggttacc tgtcgttttc gaattatcct gaaattttta
    16381 tgcaattcat agaatcttcg ttcaatttag atgataatat ctacgtaatt caacccgaca
    16441 ataaaataat ctttcggaaa caggaaatcg atatccatcg actcgagatc acttcagaaa
    16501 acgagagttt atacatcgat ggagcctaca gctccgatag cactaaaatg cttaatgcac
    16561 aattccataa tttcagtctt tctcatgccg acttcttttt gcagcccttt ggtttacaaa
    16621 taggaggctt gcttcaagga cacatccagc ttcatcagga agataacaaa cctttgattc
    16681 aggccgagat gcgaatcgat catctggctg tcaatcaaca aaatctgggt tttatggaac
    16741 tcacttcctt gtggaataca aaatcgaaag ccctctttgt aaaggctaaa tcaactctgg
    16801 ttaacaataa atttcaatct catcccatcg atttatccgg atattattat ccttcagatc
    16861 caaaacagaa tttcgacatc aaactgaaaa tggaaaattt caatctgaag cccattcaac
    16921 cttttctaaa cattgctatc gctgaactcg aaggttttct aagctcgaat atcgaactcg
    16981 aaggctcagt taaaagtcct gaaattcatg gaaagttgaa gtttattcgt gccaagattc
    17041 gtccgaaatt tttcaatact acctattctt tttcggatga aattatagtc gaaccttcga
    17101 gaataatttt caacaaactg aaaataaatg attcgttggg gaatgttgct ttttgtgatg
    17161 gatatattgc ccataagaat ttccaggata tgtatttgga tattcgattg caacctgaaa
    17221 atttcatttt gttcaatgcc gagcctcaac aaaacaaata tgtgaatgga attgccttta
    17281 tttcgggtca aattcgcgtt tcgggttcta taaataactt gaatatcgaa gccaatgcca
    17341 ttaccgagaa gggaagcaat atttacgtgc cattgtcgtc gacggtggat gttcgttctt
    17401 cgagtttctt atattttaaa caacccgaag atactttgaa gttgaagaag gtggaacgta
    17461 ccaatttgaa tgtttctggt ttcaatgttt tttcggtatt gacactaact cctcaggcca
    17521 ggataaaagt aagccttcca caagatgccg gaaccataga ggggacgggc gaaggtatga
    17581 ttacgttaga agtaaattcg agaggcgatc tcaatatcaa tggccaatat gaaattcaat
    17641 caggttcatt cctgtttaat ctgcaaaata tcctcagccg aatgctcgaa atcgagaagg
    17701 gttcgaccat acatttttct ggcgatccgt tggatgctca aataaattta agagcttcat
    17761 ttaccacgcg tactaccctt agtggattgg gattagacct cgattctacc gtcacttcgg
    17821 ctcgaatacc tgtaaaaacc attatacatc ttcaaaataa gctgttgaat ccttttatca
    17881 ccttcagcat tcaatttcca aaaatcgatc ctgacatcga acgagccgtt tatgccaaac
    17941 tcgatactac caatcagatg gttatgactc aacagttcgt atcccttctg gtgttgaata
    18001 attttagctt cgcagttacc aataaatcat ttggaaatag cttgggaatc tcttcgttcc
    18061 agatgctttc gaatcaaatc agcaatctat tgtctcaaat tagtcaagat ttcgatatcg
    18121 ggataaatta tcggcctggc gatgctattt cggctcagga actcgaactt gccctaagca
    18181 cccaactttt tgatcaacgt gtaactatcg acgggaattt gggcgtggtg ggaatgcaaa
    18241 atgcaaacaa aacaagcaat gtggtgggtg atatcaatgt agaagtaatg ctcacacgcg
    18301 atggtaaact tcgtctcaga gccttcaacc gatccaatac tactgaatta cttaccgttt
    18361 cggcccctta tacccaaggc attggactgt tttacagaaa agattttaat aatctggccg
    18421 aactgttcag gaaaaaacgt aaaatcattc aggaagaatc tctgaatatc tctacctctg
    18481 ccaattaaac cgatagaatc ttatagatca tttccttcat catttccacc ggatcagcct
    18541 gatcgatatc tacacccttg aatttcaaat catattcgct tataagttgc aaaacctgac
    18601 tgcattgagg gagattgaaa tttcgcgcgg ctgcagcata ttctttcacg aaaaaaggat
    18661 ttactcccag gataggagca gctactgagg cttttttatc atttaattga tgataaatcc
    18721 ataatttttg gaaataatta aataaaactg tactgatttt tataggagga ttttcttttg
    18781 gattgctggc aaaataaaga acgatttgag cagtgccgaa tttgttgcgt tgtgaaatag
    18841 tcctgcataa ttcgaaggga ttgaaatcct tgctaatgcc aatgtatttt tcgataatct
    18901 cgggggttat atttgaccct ttgggcagat tgatgatgag tttggcgcat tcgttaacga
    18961 ttaagctcaa gtcgttgcca aggtcgtcgg ccatcatctg gcaggcatga tctgtaatag
    19021 tataacccct ggcactcaga tattttcgaa tccattgtgg aatcttatca tcgtaaagtt
    19081 ttttcgattc aaaggatact ccgaatttat cgacggcttt tttgaaatct ttcaacaatt
    19141 catgtttcct gctggattgc aatttcaagg caactgtcaa aattgtagaa gagcttggat
    19201 gaagggcata ctggcttagc tggtccagtt ccgagcgtaa ttgctgcgct tctcgcacaa
    19261 taacgaggtt gtaatcggcc atggttggat aacggcgagc cctgtctacg attgacgaac
    19321 cagtagtatt taaaccatag aataccgaaa gattgaaatc tttttcttct tccttgagaa
    19381 tattccctgt catgtagtcg gtcagaaaat cgatgtaaaa ggattcatcc ccgtaaaata
    19441 gataaagggg tttataaatc ttgtttttta aatcggtata gatttgttca aaagtcattt
    19501 tgaatagaaa atcggggaga atttattcga ggtcgaagat cagatgtttc accgtgagac
    19561 cattattgat gagctggcgt agtgaatcta tcccaatgcg aaggtgaagt tcggtaaaat
    19621 gaagagaaac ttcccggtct ttttcttcgg ttttcactcc atcgggaatc atgggttgat
    19681 cggataccaa aagtaaggcg cctgtagaaa tatgattggc aaagcccgtg acgaaaagtg
    19741 tggcggtttc catatcgata gccatacaac gtgttttacg gaggaaaaac ttgaattcct
    19801 cgtcatgttc ccacactctg cggttggtag tataaaccgt acccgtccag taatcttttc
    19861 cataatcacg tatggtagtc gaaatggcct tttgaagtgc aaaagctggc agggcaggta
    19921 ccatttcagg catgtaatcg ttcgaagtgc cttctccgcg taaggctgca ataggaagaa
    19981 taagatcacc aatattgttt ttcttcttca atcctccaca tttgcccaaa aataacaccg
    20041 ctttgggctg tatcgaactt agtaaatcca taatggtagc ggcattggca ctccccatcc
    20101 tgaaattgat catgctgata tcacctgcac aggcaactgg catggtccga tctaatcccc
    20161 ttactttcac tcccatgatg ctggagaata aattcagata atccatgaaa ttggtgagta
    20221 aaatgtattt tccgaaatcc tccagaggaa cccctgtata tcttggaagc cagttcttta
    20281 ctatttcttc tttcgttttc atccttgaaa gatttatgat gatcctcagt ccaaagcaaa
    20341 attaggccat ttttatttag tacttttcaa ttttctcatt tcctgaaaat tcgtcattaa
    20401 ttaatgtctg gcaatgaatt tcggttgaat tgcatttcct ttgcatcttt ttgtattcgg
    20461 ttttgaagaa atcgaacgaa ctctctgtct gattttttcg attgtcgaat aaatcgggat
    20521 tatccgtcga aaacttcgaa atgcaagcgg cctcgatacg ctctgacgat ccacctggcc
    20581 accgaatata ggatttcctg tccctggtta tgatggattt tttattgatg gggaatgaat
    20641 tttaacgaaa tttgtaaagc tcatgaaaat aatgacgcgc acaatcgacg gccgagagga
    20701 gatcttcgat gaatttcgtg gccggtatgt tgaattgacc cctgaagaaa gggtgcgaca
    20761 atggtttgct cattttttgg tcgaagattg tgcttatcca cgaggtcgta ttggaataga
    20821 aatttcaatc gtcatacagc gtaaaactta tcgcagcgat ctggtggttt tcgacaagaa
    20881 caggcgtccc tggatgttgg ttgaatgcaa gtcccctgag atagccttga ccgaaaaagt
    20941 gttcgaacag gtaactctgt ataattatac cctgaaggct gattttattg tgctcaccaa
    21001 tggcaattcg actataataa tgaaactcga ttatgctgca ggaaaaatcg aattccagtc
    21061 cgaattgcct ccctatccca aggattaaat tttttgttta gtgagtatgg taggatttta
    21121 ctttctgatt ttttagagtt tcgaaaagtt ttcggcccgc cgaaggcggg ccgcataatc
    21181 aaccaaaaca ctcacagaag cctgcttttt atatgtaccg gaagcagaac cggtgaattt
    21241 ataggcaatt aattccttat tcacctaatt tataacgtaa ccctacttgc agacctaaag
    21301 tggagttatg cgaattagct cgatcgggat tgtatcccaa tgcatcctta ttttccggat
    21361 tggtaaaatc ataatcgaat ctcaccatgc caaatacatt gaatttggaa ttaatttgat
    21421 attctccccc agctccaata aaaccaccaa aagtcaaatt ttcgtaatga tgcttgatgt
    21481 ccatttcgtt gtcattggca tcgagatact tggcagtggt cagaatattg aattgaggcc
    21541 ccacatttag gctgaacatc cacttctgag caggattagt gttgaaatgg aaatacagag
    21601 gaattttgac gtaatcgagt tttatacgcg tttccactcc acctacttcc tgtttgcgtc
    21661 cctgaaacga atacattacg tctaatccca ttcctaactt ttcggtaaag ttgtatcccg
    21721 cacctatgct taaagctgga tggatataag ctttctggga aaaattatcc atatcactgt
    21781 catccgaatt aaacaaccat gaattttgaa ccatagcgtt tacattgaat gaataaccct
    21841 tttgtgcttt cgtttgtacc gaaaaagcaa ccatcgtaat catcaacaat gctgctaaga
    21901 ccaccctttt gtttttcata gctttttctt gttttagtta gttattaatt ttttaatttc
    21961 tgtctttcaa ccccaattat tgcagaaaaa cttgcaggct tctgctgata tcttgattca
    22021 tcattcctga accatttttt gaaatattct tctgctgtaa ctgatgaaag aaacacgtta
    22081 cgaatttctt ttcatccaga ttggtgccga aaaactttca ctttttgata tttcgctctt
    22141 accgcctcca aatttgtgcc agattcgtag tattcccgtt gaacttcttt tccatattga
    22201 tttccattca gatactatac tcgaactatt ttttgtgata aaagccataa tgtcagttat
    22261 tgctcttttt ttcgatgatg aaagcgtcga atcctttttc tcttagcata cttcgaaagt
    22321 tttttgcttc gatatagtcg gaaaatctgc ctattcttac tttgtaaagt ttgttttcat
    22381 attttatcca gacaggtaat tcaaaggttc ttttcagggt ttcggaaagt tgttcggcat
    22441 tatgcttatg tttgaaagcc cccgtttgaa gagtgtaaaa ctcttgaaca agcggtattc
    22501 ttatttctgt aacttttaga gtgtcttttg gggtttcggt aatttttttg tcctctgttg
    22561 gaggtctttc gacgaaaaag ttcaaatcgt aaaccacatc gcccagaacg tcgggtttga
    22621 tttcgaaagg tatttcggaa ggagatgaca ctatgccaag ttttgccact tgagccgaat
    22681 ctaagcgggc tatgtaatgg ccaggaggta agccaagata actaaatgcg ccatctcttt
    22741 cggtaatcgt agaagctatc tttttcccta tggacgaata atatcgatga tgatgtgtcc
    22801 ttcgcctctt tggccggtac cgtctttcct caatacccag ccgtttattt ctcccatggg
    22861 cagtacagga atctctattt ttttgaattg attaggctcg gcataaatgc gaatgacttt
    22921 atgcgcaata cgataagaaa tctcttcgag acttttttcg tctatggtaa ggatatagtc
    22981 ggtataggga attaaaccca ccacacggat aatagaatct ttttccccct gtatgacacg
    23041 accgccattg atgcgcatgg caagcccctt aaccaagcgt tcgccactgt ctcttttgta
    23101 attgtgattg aggtctataa aagaaatgat ggataatcct cccattccta cggaaggacg
    23161 atcttcggca tgcacatagc ctttcccgct tccaaaggca aaactgccct gagctccttc
    23221 ggtaatcata aattcgttgt tcgacaagcg agtagctaaa tttacctgcg aaaaaggaac
    23281 agtccatcga aaagaaaggc taattgcacg atatttcgat tggaaatttt cctcgcccat
    23341 tagggaaagg tatccctgcc gcaatatccg tttctctatt tcggctctga gagaaataac
    23401 cctattgtcg ctaaaattga tttgcgattg tacgcgggag gtaaatcctt tccattgagg
    23461 c
//
LOCUS       DAOORU010000017        22229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_2849_length_22229_cov_4.824344, whole genome shotgun sequence.
ACCESSION   DAOORU010000017 DAOORU010000000
VERSION     DAOORU010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22229
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2849_length_22229_cov_4.824344"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..442
                     /locus_tag="PLF20_02345"
     CDS             53..442
                     /locus_tag="PLF20_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOX79881.1"
                     /translation="MSQIKSFEDLRKIKEDFGKRIQFREKSFDPSKVVQIKVGFGTSG
                     IASGAKEVMTFLRDELEKRNIEAVVTQTGDMGYSYAEPTIEVKLPGQEPIVFGKVNVR
                     KADEIIERYIKNGELIDGIIPVNYHTI"
     gene            468..2261
                     /locus_tag="PLF20_02350"
     CDS             468..2261
                     /locus_tag="PLF20_02350"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOX79882.1"
                     /translation="MAKYRMHLLVCGGTTCRSSESESIRNALEESLERNGLENEVKVV
                     STGCFGFCDKGPIVKVIPGNTFYIHVKPEDAEEIVKEHVLNGRRVERLLYLEPEKELL
                     ATGSKEIGFQKKQIRIALRNCGFIDPENIDEYIARDGYVALCRCLTEYTPQQVIEMIK
                     KSGLRGRGGGGFPTGLKWEITAKVKSDQKYVICNADEGDPGAFMDRSILEGDPHSVIE
                     AMAINGYCTGASKGLVYIRAEYPMAIKRLRIAINQAKEYGLLGKNIFNSGFDFDIELR
                     YGAGAFVCGEETALIHSMEGKRGEPTFKPPFPSEHGFLGKPSNVNNVETYANIPVIIN
                     KGPEWFASIGTEKSKGTKVFALAGKINNVGLIEVPMGTTLRDVIFKIGGGIRGGKGFK
                     AVQTGGPSGGCLTTRHLDTPIDYDNLLAAGSMMGSGGMIVMDEDDCMVSIAKFYLGFT
                     VDESCGKCSPCRIGNKRLYEILDKITKGEGTTEDLAKLKNLSEVIKDTSLCGLGQTSP
                     NPVLSTLDNFWDEYVEHVIDKKCRASSCKGLMKYVIDPEACTGCTACARACPVNAISG
                     ERKGPHTINQEICIKCGTCMEKCKFNAVLLQ"
     gene            2291..4060
                     /locus_tag="PLF20_02355"
     CDS             2291..4060
                     /locus_tag="PLF20_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOX79883.1"
                     /translation="MELVKLTIDKKSVEVEKGTSILNAARKVGIEIPTLCYFNLEGMN
                     IENKPGGCRICVVEVQGRRNLAPACATEVTEGMVVKTNSTRVLNARRTVMELILSDHP
                     FDCLVCPKSGNCELQSLAHRLGIRDIPYQGEQSTYRKDTSPSIIRDVDKCILCRRCEM
                     MCNDIQTVGALSAINRGFMAVVAPAFEMNLEKSPCTYCGQCVAVCPTGALTEVDNTRQ
                     VISALADPTKTVIVQTAPAVRAALGEEFGLEPGSLVTGKMVSALRELGFDYVFDTDFA
                     ADLTIMEEGSELLDRLTRYLSGDRNVRLPILTSCCPAWVNFFEHNFPELKDVPSTARS
                     PQQMFGSIAKTYFAKKLGIDRNNLVVVSIMPCLAKKYECQRSEFLTDGNPDVDFSLST
                     RELANFIKLANIDFNSLPESDFDRPLGESTGAAVIFGTTGGVIEAAVRTAYELYTKKP
                     LPRVDFEELRGLEGVRAATVNFDGLELRIGIAHGLGNARQLLEEVRDGKSQYHAIEIM
                     ACPGGCIGGGGQPLHHGDASILKARQKALYEEDRNKALRKSHENPYIIQLYEEFLGQP
                     LSEMSHHLLHTQYFDKSTQIVIE"
     gene            4095..4577
                     /locus_tag="PLF20_02360"
     CDS             4095..4577
                     /locus_tag="PLF20_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOX79884.1"
                     /translation="MAVIKLPESKVQQLKNICKSFNNDKGELINVLHKAQEIFGYLPA
                     EVQEVVAKELNVSVAHVYGIVSFYSFFSMVPKGKFSISICLGTACYVRGAEKVLDEFR
                     KQLNIQVGESTPDGRFSLSCLRCVGACGLAPVVMIGEKVYGRVAPEDVKGIINEYANL
                     "
     gene            4723..7983
                     /locus_tag="PLF20_02365"
     CDS             4723..7983
                     /locus_tag="PLF20_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOX79885.1"
                     /translation="MRKIANLLFVFLIFITFGLKAQEEGRLMRFPTIHGNQIVFSYAG
                     DLYTVDKSGGIARKLTSDANGYEMFARFSPNGKWIAFTGQYDGNTEVYVIPAEGGIPR
                     RLTYTATLQRDDISDRMGPNNIVMTWRDNESIVYRSRKQSFNDFKGQLFVVNIQGSLS
                     QEVPLPSGGFCSYSPDKSQLAFNQVFREFRTWKYYKGGMADDVWIYDFKTKKTQNITN
                     NIHQDIFPMWKDDFIYFLSDRDRTMNLFCYNIKTSQTEKLTNYTEFDIKFPSLGDDAI
                     IYENGGYIYVFDLKTRRAQRVNILLGDDFLLSRDTWKDASKSINSWDMMPDGKRAFFD
                     ARGDIFSVPAKEGVTFNLTSTSAIHERDIACSPDGKYIAYISDRNGEDEIYIQPADGS
                     GTAEQLTHLGDTYKYSMLWSPDSKKILWSDKLQRLQYIDVNTKKITLVDQTLEGEFNS
                     FTWSPDNRWIAYTLPSTRTSSRIFVYEVATGEKHFATDRWYDATSPAFSPDGKYLYFA
                     SNREFNPIYSWTEWNHAYIDMQKIYLVPLSKLTTSPFAPTTELNASAIEKKPEPQAKK
                     DETKSPKASPEVKIDFDGIETRSIAIEVDPGRFWNLAPTEKGIYYNRTNSKESKALLC
                     YFDLNNKKETVIGEYHAFVLAPDGKKMMIAARGKYYIIDAPQSKIQPEESLNLSDMKV
                     FVNVKQEWAEAFKESWRQMRDFFYDPNMHGVNWKAIYQKYEPMVKYVNNRNDLNYIIG
                     EMVGELNIGHAYVGGGDKPEPQRIKTGLLGARLSKDASGYFKIDKILSGENWDPSLRS
                     PLTEPGLNIQEGDFILEVNGQPTNQMLDIYTSLVGKAGSLVELSVASTPDPSAARKVI
                     IKPIESESGLYYFNWVRSNIEKVNKATNGQVGYIHIPDMGIAGLNEFAKYFYPQLDKR
                     ALIIDDRGNGGGNVSPMIIERLRREVATFSVQRNGAVTTRPSQMIWGPKVLLIDRYSA
                     SDGDLFPYQFKYYKLGKTVGQRTWGGVVGIRGTLPIIDGGMLNKPEFAPYDVEGKSFI
                     IEGYGVEPDIEIWNDPAKEYEGVDEQLNKAIEVILEELQNWPEQFPPVPPYPDKSK"
     gene            complement(8169..9173)
                     /gene="floA"
                     /locus_tag="PLF20_02370"
     CDS             complement(8169..9173)
                     /gene="floA"
                     /locus_tag="PLF20_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861713.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HOX79886.1"
                     /translation="MSSTLLIIIIGLVALFGLWIIFYFIPVGLWFSALVSGVRISLIQ
                     LILMRWRKVPPSVIVNNLIAATKAGLKINANDLETHYLAGGRVKSVVNALISAEKANM
                     GLDIKTATAIDLAGRDVFEAVQMSVQPKVIDTPPVTAVAKDGIQLIAKARVTVRANIR
                     RLVGGAGEETILARVGEGIVSSIGSAESHKIVLENPDSISKLVLQKGLDSGTAFEILS
                     IDIADIDVGKNIGAVLQMDQANADKNIAQAKAEERRAMAVALEQEMKAKAQEARAKVI
                     EAESEIPKAIAEAFRNGNLGIMDYYRFENLKADTKMRESIATPPAKPKNTKDDDLTQQ
                     "
     gene            complement(9184..9648)
                     /locus_tag="PLF20_02375"
     CDS             complement(9184..9648)
                     /locus_tag="PLF20_02375"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOX79887.1"
                     /translation="MSWSIIIILIVVGLIFMVLEILVIPGTSVGGIIALACVGIAIWQ
                     SFAAHGMIAGSITLGITLAVSFIVLYFSLKSNTWKRLMLTQQNTAKVNVINEDIVKPG
                     IQGLTLSRLAPAGKIEIEGQEYEAHTFGEYVDPGQKVIVVKIEYNKIYVKPI"
     gene            complement(9653..9952)
                     /locus_tag="PLF20_02380"
     CDS             complement(9653..9952)
                     /locus_tag="PLF20_02380"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HOX79888.1"
                     /translation="MAGKIWEDYMEETKEKTIAQEVTGVIGVKDLILFNDDVNTFEYV
                     IECLMEVCQHTLEQAEQCATIAHLKGKCPIKMGCYEELKPICDELIRRKLTVAIK"
     gene            10278..10919
                     /locus_tag="PLF20_02385"
     CDS             10278..10919
                     /locus_tag="PLF20_02385"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOX79889.1"
                     /translation="MKNFTLDIDELQGRLSYHLPGWEAQKNMAPTGRVHIPPADMVPI
                     ESAVLLLLVKNSADYEIVFIQRTEDDGPHSGQISFPGGRREPHDKTLLDTALREATEE
                     IALKCNHLKVLGQLTPLYVPTSNFMIYPFIGFADGCDHFRPQPDEVQKVIRVRLNDIL
                     DRNHRRIEVFTSSAGDSYKAPAYFIEEVRIWGATAMIVSEFCEIISSLDRENR"
     gene            11349..12035
                     /gene="mqnB"
                     /locus_tag="PLF20_02390"
     CDS             11349..12035
                     /gene="mqnB"
                     /locus_tag="PLF20_02390"
                     /EC_number="3.2.2.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR03664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="futalosine hydrolase"
                     /protein_id="HOX79890.1"
                     /translation="MRILLVTATWLEAKPLMDGFTFLRKYSGNNYSFRYGEHRIDVLV
                     TGMGMVATAFGVGKALRNTQYNLVLDAGICSSFRKEFSPGTVLNVSEEFFPEFGMSDD
                     AGFKYLQDMGFDTSLPTACKNTVLQNPHYNQVMGFKNLATARGATVNLVNLTGRRLNT
                     YHSNIEADIETQEGGAFFFSCLASEVPFIELRAVSNYIGPPDEEGWDIEKAIQNLTYS
                     VHEALNVLPR"
     gene            12084..13058
                     /gene="rfaD"
                     /locus_tag="PLF20_02395"
     CDS             12084..13058
                     /gene="rfaD"
                     /locus_tag="PLF20_02395"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HOX79891.1"
                     /translation="MQKKIIVTGAAGFIGSCLVGKLNELGYENLILVDDFSSILKEPN
                     WVNKKYEMCIPREDFLQGVHECSCDIEVVFHLGARTDTTEMNEKVFDALNLTYSKQIF
                     NFCSQYRIPLLYASSAATYGAGELGYSDDHHLIPLLRPLNPYGISKNEFDKWVLQQNI
                     TPPFWAGFKFFNVYGPNEYHKHRMASVVFHSANQIRNTGKVRLFRSHRPDYADGMQKR
                     DFVYVFDVIETLIRFWQHRPVSGIYNLGTGKAEPFLRVAEAVFEALEFSPAIEFIDTP
                     IDIRDKYQYFTQADIRKIQATGLTPDFTSLYDGIIDYVGRFLKYQKYY"
     gene            complement(13090..13503)
                     /locus_tag="PLF20_02400"
     CDS             complement(13090..13503)
                     /locus_tag="PLF20_02400"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HOX79892.1"
                     /translation="MSFYHLLDWFFTIFHSSLMIFNLIGWCFTKLRKLNLITLLLTAS
                     MWTIAGIWYGLGYCPLTDWHYDVLRLLGYQNLPNSYLQFITHRLTGINLSTPVADIIT
                     VSGLILSLVISIILNYRDYQNKRKARKETPKILED"
     gene            complement(13512..13853)
                     /locus_tag="PLF20_02405"
     CDS             complement(13512..13853)
                     /locus_tag="PLF20_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOX79893.1"
                     /translation="MTSQNNQKKKQHLYYTIGEVSRMLDVNPSTLRYWEKEFDGIHPY
                     RNRKGTRFYHTEDVETLRLIKYLLKERGFTIAGARDYLKTSRSHVLRNMDIVETLKNI
                     REFLIQLRNNL"
     gene            complement(13885..14874)
                     /locus_tag="PLF20_02410"
     CDS             complement(13885..14874)
                     /locus_tag="PLF20_02410"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOX79894.1"
                     /translation="MRRKIRYYFDPLSLTYQIIPVRFRDRLKRILYILATGLVFSAVV
                     IALAFKFLSSPKERILQREIEQYKVQVQAMNYELDQMQKILTDLQNRDDNIYRVIFET
                     EPVPASIRQAGIGGANRYQNFEGSDNFALLLETAKKLDRVKSQIYVQSKSFDEVFEMA
                     KKKEAMVTAIPAIQPVSKRDLKYISSYFGYRIHPFYKTVMFHEGIDFTAPKGAKVYAT
                     GDGVVKEASLINLDGFGKKIIIEHGFGYQTIYAHLNSFNVKPGQKVKRGDIIATVGTS
                     GLSTGPHLHYEVIYNHQKVNPAYFMFDLDPATFQEIIRQSSDYKESSNIPVGE"
     gene            15090..17720
                     /gene="alaS"
                     /locus_tag="PLF20_02415"
     CDS             15090..17720
                     /gene="alaS"
                     /locus_tag="PLF20_02415"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOX79895.1"
                     /translation="MKSADIRKTFLDFFAERQHQIVPSAPMVVKNDPTLMFVNAGMNQ
                     FKDIFLGNRPVVFPRVADTQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYFK
                     EEAIAWAWELLTEVFKIDGSRLYVTVFQGDDADYLPPDQESYKIWQHYQTEEKILFGG
                     KKDNFWEMGDTGPCGPCSEIHIDVRSGEERNAVPGYTLVNKGHPKVIEIWNLVFIEFN
                     RMANGQLQQLPARHVDTGMGLERLCMVLQGKQSNYDTDVFQPIIREIEKLSGKYYGSD
                     PRIDVAMRVIADHLRAVAFAIADGQLPSNNKAGYVIRRILRRAVRYGYNFLGFRQPFM
                     VSLVPILRDQMGSFFPELIAHESLISNVMAEEEANFLRTLESGIQKFNSYISNNQSKG
                     IIDGNFAFELYDTYGFPIDLTQLMAAEQGWEVDMPSFYQHLEEQKQRSRADAAINTDD
                     WIEVVSGLGETTFIGYDTLEAEAEIIKYRYVSTAKKQYFQIVFNHTPFYAESGGQVGD
                     RGWIIAGDEKIEIENTIKENNLVIHIVSRLPLDVSQPVLLKVDEVKRRLTANNHSATH
                     LLHAALRKVLGTHIEQKGSLVDENRLRFDFSHFTRLTSDEILKIEAMANGKIWENLPL
                     KEWRNLPMEEAQKMGAMALFGERYGEMVRVVAFDPEYSVELCGGTHVKATGQIGLIKI
                     ISETGIAAGIRRIEAVTGTVAVAFYNQQLQILEEIKTLVKNPVDALKGVKNLLEEKQK
                     LTLEVENLRLYKINSLLQILSENIKKANGINFLSIELDVDVQMAKDLLFDLKRRFSPF
                     VGLIAYINQNKPGFSLIVSDELIETGKVDARELVKVLSPCIKGSGGGQAGFVTAGGSF
                     TKGVSELLDKAKQLFAQIDS"
     gene            complement(17697..19736)
                     /locus_tag="PLF20_02420"
     CDS             complement(17697..19736)
                     /locus_tag="PLF20_02420"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOX79896.1"
                     /translation="MLRENPILKLFIPLITGILLSIYFSRIRFSPILCGIIFLIAAIY
                     LLFFYIRLSYKNQWIYGLMIHILMFLSGVSVVSLRELSFKHEHFSSDKTSEYFQAIIE
                     DYPQAKAKSYKVIVKIYGLYDSARWKPVSGKIMTYLEKTPEAGELKPGTRILFKGNYF
                     EIANSGNPGAFDYKSYLARRGIYHQLYLSQNDWKVIGDAHAFSIKTLSAKARMQLLKM
                     MKNSGVSGDEYSVAAAILLGNSDYLDQELRQKYSGSGTMHILAVSGLHVGIIYLILFY
                     LLGFLSKIPHGRLIRSIIVVFAIWIYALITGLSPSVTRAATMFSFVSMGEIFKRRTPI
                     FNTLAASACFILIFNPFLIQEIGFQLSYLAVLGIVVTQPGIYRMLNFKNKIADKIWGL
                     ICVSIASQIATFPLALFYFHQFPVYFIISNLLVIPISYVIMMLGVAFFMILPFGHAGF
                     YAGKVLSGSIWLMNQSVGWVESLPGAVASGIYITAAQYLVLYFVIIMLILFFSKNIKS
                     YFQAALAGIFLFFFIDTADRLYRSRQQSMYVMNSPRIKGPWLVNGIDGLLFTSPSEGY
                     SESQQIQQSLKIKNIQILSESNLLRWNGKLIYYIPDGTTYADQSPVKPDYCIISGNPA
                     FHVQEFLSHIHPQMIIMDGSVPRWICTEWEKTLKELDVSYHAVPLDGVLRIDLSE"
     gene            19967..20971
                     /gene="gap"
                     /locus_tag="PLF20_02425"
     CDS             19967..20971
                     /gene="gap"
                     /locus_tag="PLF20_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOX79897.1"
                     /translation="MAKARIAINGFGRIGRLTFRALLEKEEVEVVAINDLTDSKTLAH
                     LLKYDSVHRRFPGTIEVDGDYLVVNGNKIRIYAEKDPLNLPWKDLSIDVVVESTGVFR
                     TREKMSKHLTAGAKKVALSVPADNAEDVDITVVIGVNDHQIRPEHKLISNASCTTNCL
                     APVAKILHEKFGIVRGLMNTVHSYTNDQVILDAPHKDLRRARSAAMSIIPTKTGAAKA
                     VALVIPELKGKLDGFAMRVPTPDASIVDLTAELAVKTTKEEINTAMKDAAEGKLKGIL
                     EYCIDPVVSSDIIGNKHSSVFDSLLTQVIEGNFVKIVAWYDNEMGYSQRLADLVIKML
                     "
     gene            complement(21059..21925)
                     /locus_tag="PLF20_02430"
     CDS             complement(21059..21925)
                     /locus_tag="PLF20_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOX79898.1"
                     /translation="MRQLKIAKSITNRENASLDKYLQDIGKEEMITVEEEVKLAQMIQ
                     EGDQKAMEKLCKANLRFVVSVAKQYQNQGLSLPDLISEGNAGLIKAAQKFDPTRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNKIKKEASRLEQKLERPPSAEELAE
                     AMDLSESKVDAVLKVNTRYVSMDAPLQDDEDTKFIDVFVAEDTPATDENLINESLKAE
                     IDRSLSTLSPKEREIICLYYGIGQNYALTLDEIGAKFNLTRERVRQIRERAIRQLKHT
                     SRSRLLKAYLGQ"
BASE COUNT         6594 a         4249 c         4784 g         6602 t
ORIGIN      
        1 agaaattgat tgtgttaatt ttaacatcat aaaccttaaa aatatccacc acatgtctca
       61 gataaagtca tttgaagatt taaggaaaat aaaggaagat tttgggaaaa ggattcaatt
      121 tcgtgagaag agcttcgatc catcaaaggt agttcagata aaagttggat ttggcacaag
      181 cgggattgct tcgggtgcga aagaagtgat gaccttttta agggatgagc ttgaaaaacg
      241 aaacatcgaa gccgtggtta ctcaaaccgg agatatgggt tacagttatg ctgaacctac
      301 gatagaagta aaacttcccg gtcaagaacc gatcgtattt ggcaaggtta acgtcaggaa
      361 agccgatgaa atcatcgaaa gatacattaa aaacggcgaa ctcatcgatg ggatcattcc
      421 cgtgaattat cacaccatat aacataagta gtaaaacacc ataaaaaatg gcaaaataca
      481 gaatgcattt actggtttgc ggagggacaa catgtcgctc gtcggagagt gaatctatac
      541 gaaatgctct tgaggaatcc cttgaacgca atggcttaga aaatgaagtc aaagtagtgt
      601 caacgggctg ttttgggttc tgcgataagg gtcctattgt gaaggtgatt cccggtaata
      661 ctttttatat tcacgtgaag cctgaagatg ccgaagaaat tgtgaaagaa catgttttaa
      721 atggtcgtcg ggtcgaacgt ttgctttatc ttgaacctga aaaagaactt cttgctacag
      781 gtagcaaaga gatcggtttt cagaaaaaac agataaggat agctttacga aactgtggtt
      841 ttattgatcc ggaaaatatt gacgaataca ttgctcgcga tgggtatgta gctctttgca
      901 ggtgccttac tgaatacact ccacagcagg tcatcgaaat gattaagaag tcgggattac
      961 gtgggcgtgg aggtggtggt ttccccacag gtttgaaatg ggaaattacg gcaaaagtaa
     1021 aatcagacca aaagtatgtg atttgtaatg ctgatgaagg cgatccgggt gcatttatgg
     1081 atcgttccat tctggaaggc gatcctcatt cggtcatcga agccatggct atcaatgggt
     1141 attgtacagg cgccagcaaa gggctggttt atattcgtgc agaatatcct atggccatta
     1201 aacgtctcag aatagctatt aatcaagcta aagaatatgg tttacttggt aaaaatattt
     1261 ttaattcggg gttcgatttt gatatagaat taagatatgg ggctggagct tttgtttgcg
     1321 gagaagaaac cgcgttgatt cattccatgg aagggaaacg aggtgaacct accttcaaac
     1381 cccctttccc atctgaacat ggttttctgg gaaagccgag caatgtgaac aatgtagaaa
     1441 cttacgccaa cattccggtt attatcaata aagggcctga atggtttgct tctattggaa
     1501 cggaaaaatc gaaagggacc aaggtatttg cacttgccgg taagatcaat aatgtaggct
     1561 tgatagaagt tcccatggga acgacccttc gagatgtaat ctttaagatt ggtggaggca
     1621 tcaggggagg aaaggggttt aaagccgtcc agacgggtgg tccttcagga ggttgtctta
     1681 cgactcgtca tctcgatacg ccaatcgatt acgataactt gttggctgcc ggctcgatga
     1741 tgggctcggg cgggatgatc gtaatggatg aagacgattg tatggtttcc attgctaagt
     1801 tttatcttgg atttacagtg gatgagtcgt gtgggaaatg ctctccatgt cgaattggga
     1861 ataaacgctt atacgaaatc ctcgataaga ttactaaagg agaaggcacc acggaggatc
     1921 tggccaaact caagaatctc agcgaagtca ttaaggatac ttccctttgc ggattgggtc
     1981 aaacttcacc taatcccgta ctttctaccc tcgacaattt ctgggatgaa tacgtcgagc
     2041 atgtaatcga caagaaatgc agggcaagtt catgcaaggg cctcatgaag tatgtaatcg
     2101 atcctgaagc ctgtacggga tgtacggcgt gtgcaagagc ctgtcccgta aatgctatat
     2161 ctggcgaacg taaggggcct catacaatca atcaggaaat ttgcatcaag tgtggaactt
     2221 gtatggaaaa gtgcaaattc aacgccgttt tacttcaata aaatgttgaa tctttaacaa
     2281 atttcgaaac atggaactcg taaaactaac catagataaa aaatcagtcg aggtagaaaa
     2341 aggaaccagt attttgaatg ctgcccgaaa agtgggcatc gagatcccaa cactttgtta
     2401 tttcaatctg gagggaatga atatcgaaaa caaaccaggt ggttgccgta tttgtgtggt
     2461 tgaggttcag ggccgtagaa atcttgctcc ggcgtgtgct actgaagtaa ccgaaggtat
     2521 ggtggtcaaa accaacagca ctcgcgtatt gaatgctcgc cgtaccgtaa tggaactcat
     2581 cctcagcgac catccttttg attgtctggt ttgtccaaag tcgggcaatt gtgaactaca
     2641 aagcttagct catcgtttgg gaatccgaga tattccctat caaggcgaac aatctactta
     2701 ccgcaaggat acatctccaa gcataattcg cgatgtggat aaatgcatcc tgtgcagacg
     2761 gtgcgaaatg atgtgcaacg atattcaaac cgtaggtgcc ctttcggcca ttaacagagg
     2821 gtttatggca gttgtagcac cggctttcga aatgaatctc gaaaaatctc catgtaccta
     2881 ttgtggccaa tgtgtagcag tttgtccaac gggtgcactt accgaggtgg acaacacccg
     2941 tcaggtaatt tcggctttgg ctgatccaac caaaactgtg atagtacaaa ctgctccggc
     3001 tgttagggcg gctttaggag aagaatttgg cctcgaaccc ggatccctgg tgacagggaa
     3061 aatggtttcc gcactgcgcg aattaggatt cgattatgtt ttcgacaccg atttcgccgc
     3121 tgatcttacg attatggaag aaggcagtga gctcctcgac cgtctcacca gatatttatc
     3181 aggcgacaga aatgtacggc ttcctatact tacttcttgt tgtcctgcat gggtaaattt
     3241 ttttgaacat aatttccctg aattgaagga tgttccttcc acggcacgtt cacctcagca
     3301 aatgtttggt tcaatagcaa aaacttattt cgccaaaaaa ctgggcatcg atcgtaataa
     3361 tctggtagta gtatccatta tgccttgttt ggctaagaaa tatgaatgcc agaggtcaga
     3421 atttttgacc gatggaaatc ccgatgttga tttttcgctt tctactcgtg aactggctaa
     3481 tttcattaaa ctggccaata ttgatttcaa ctcgcttccc gaaagcgatt tcgaccgtcc
     3541 tttgggggaa tcgacgggag ctgcagtcat ttttggaacc actggaggag tgatcgaagc
     3601 ggccgttaga acagcttatg aactttatac gaaaaagcct ttacccagag tcgacttcga
     3661 ggagctgaga gggctcgaag gagtgagagc cgctaccgtg aatttcgacg gtttggaact
     3721 gcgtataggc attgcccatg gattggggaa tgcacgccaa ctgctcgagg aagtaagaga
     3781 cggtaagtcg caatatcatg ccatcgagat catggcttgc ccgggcggat gtattggagg
     3841 tggtggtcaa cctcttcatc atggtgatgc ttcaatactt aaagccagac aaaaagctct
     3901 ctatgaagaa gaccgcaaca aagcccttcg taaatctcac gaaaatcctt atattattca
     3961 attatatgag gaatttctcg gacaaccact cagcgaaatg tcgcatcatc tactgcatac
     4021 gcagtatttc gataaaagta cccaaattgt gatcgaataa atattctctt attcactaaa
     4081 aaacattaca caaaatggct gtcattaaat tacccgaatc gaaagtccag caactgaaaa
     4141 atatctgtaa atccttcaat aacgataaag gtgaactcat caatgttcta cacaaggctc
     4201 aggaaatttt tggttacctt ccggccgaag tacaggaagt agtagctaag gaattgaatg
     4261 tctcggtagc ccatgtgtat ggtattgttt ccttttattc gtttttctcg atggtaccca
     4321 aggggaagtt ttctatttct atatgtctgg gaacggcttg ttatgtgaga ggggcagaga
     4381 aggttctcga tgagtttcgc aaacaactga atattcaggt gggcgaaagt acacccgatg
     4441 gtcgcttttc tttgtcttgc ctcagatgtg tgggagcctg cggtctggca cctgtcgtca
     4501 tgataggcga aaaagtttat ggacgtgtag ctcccgaaga tgtgaaggga atcatcaacg
     4561 aatacgcaaa tctctaagtg ttttcataat tttaggtttt ggttaatcaa aaaggctgta
     4621 tctggagtga tatggccttt tttaatttat tgtcattttt tttgcatact tgaaaggtct
     4681 ttttcaataa ctttatgcct tcaaattttt aaagcttttg aaatgagaaa aattgctaac
     4741 ctgttgttcg ttttcctgat attcatcaca tttggattga aagctcagga agaaggaaga
     4801 ttaatgagat ttccaaccat tcatggaaat cagattgttt ttagctatgc aggcgacctt
     4861 tatactgtag ataaatctgg gggtattgcc cgtaaactta cttccgatgc taatggctat
     4921 gaaatgtttg cacgcttttc acccaatggg aaatggatcg cctttaccgg acaatacgac
     4981 ggaaataccg aagtttacgt tatccctgcc gaaggtggca ttccccgtcg cttgacttat
     5041 acggcaactt tacagcgcga tgatatctct gaccgtatgg ggccaaataa catcgtaatg
     5101 acatggcgcg ataacgaatc cattgtatat cgctcacgca aacaaagctt caacgatttt
     5161 aaaggacagc ttttcgtggt aaatattcaa ggaagcctga gccaggaagt tccactgccc
     5221 tcgggtggct tttgctccta ctctcccgat aagtcacaat tagccttcaa tcaggtattt
     5281 agagaattca gaacttggaa atattataag ggcggcatgg ccgatgatgt ctggatttat
     5341 gactttaaaa ctaaaaaaac tcaaaatatt actaataata tccatcagga tatcttcccc
     5401 atgtggaaag atgattttat ttatttcctc tccgatagag acagaactat gaatcttttc
     5461 tgctataaca taaaaaccag tcaaaccgag aaattgacaa attatacgga atttgatatt
     5521 aaatttccaa gtcttggcga cgatgccatc atatacgaaa atggaggata tatttatgtt
     5581 tttgacttga aaacccgccg ggcacaaagg gtaaatattc ttctgggaga tgattttctt
     5641 ttaagtcgtg atacttggaa agatgcttcg aaaagcatca actcatggga catgatgccc
     5701 gacggaaaga gagcattttt cgatgctcgt ggtgatatat tttctgttcc tgccaaagaa
     5761 ggtgtaacat tcaaccttac ttctacctcg gcaattcacg aacgtgatat tgcttgctct
     5821 cccgatggga aatacatcgc ttatatcagt gatagaaacg gtgaagacga gatatatatt
     5881 caaccggccg atggaagtgg aacagctgaa caacttacac atttagggga tacttataaa
     5941 tattctatgc tttggtcacc cgacagcaaa aagattttat ggtcggataa actgcagcga
     6001 ttgcaataca tcgatgtaaa tacaaagaag attacgctgg ttgaccagac ccttgaaggt
     6061 gaattcaatt catttacatg gtcacccgat aatcgctgga ttgcctatac tttaccttct
     6121 actcgcacat cgtctcgtat ttttgtttat gaagttgcta cgggcgaaaa acattttgca
     6181 actgatcgtt ggtacgacgc cacctctcca gctttcagcc ctgacgggaa atatctgtat
     6241 ttcgcttcca accgtgagtt caatcccatt tattcatgga cagaatggaa tcatgcttac
     6301 atcgacatgc agaaaattta tctggttcct ttatcaaaac ttacaacttc tccctttgcc
     6361 ccaaccacgg aattgaatgc ttctgccata gaaaagaaac cagagcctca ggcaaaaaag
     6421 gacgaaacta aatcgcctaa ggcctcccct gaggttaaga tcgatttcga tgggatcgaa
     6481 acccgttcta ttgccattga agtcgatccc ggtcgtttct ggaatcttgc acctaccgaa
     6541 aaagggatat attacaatcg tactaattct aaggaatcga aagccttgct ttgttatttc
     6601 gacttgaata ataagaagga aaccgttatt ggagagtacc atgcctttgt tcttgctcca
     6661 gatggtaaga agatgatgat tgctgctcga gggaaatatt atattattga cgcccctcaa
     6721 tcaaagattc aaccagaaga atccctcaat ttatcagata tgaaggtttt tgtgaatgtg
     6781 aagcaggagt gggccgaggc ttttaaggag tcgtggcgac agatgcgcga ctttttctat
     6841 gatcccaaca tgcatggcgt aaattggaaa gccatctatc agaaatatga acccatggta
     6901 aagtatgtta acaatcgcaa tgatttgaat tatattattg gtgaaatggt tggtgagttg
     6961 aatattggac atgcttacgt ggggggtggc gacaaacccg aacctcaaag aataaaaact
     7021 gggcttctgg gggcgcgttt gagtaaggat gcttcaggtt attttaaaat tgataaaata
     7081 ctgtctggag aaaattggga tccttctttg cgctctcccc ttaccgagcc gggcttaaat
     7141 attcaggaag gcgattttat tcttgaggtg aatggccaac ctaccaatca aatgcttgat
     7201 atttacactt ctttggtagg taaggcaggg tccttggttg aactgagcgt tgcttctact
     7261 cccgatcctt cagcagccag aaaagtgatt attaaaccca tcgaaagcga atcgggtttg
     7321 tattatttta actgggttag atcaaacatc gaaaaggtta acaaagctac caatgggcaa
     7381 gttgggtata tccatattcc ggatatgggg attgcaggat tgaacgagtt tgcaaagtat
     7441 ttctaccccc aactggataa acgcgccttg attatagacg accgtggcaa tggaggtgga
     7501 aatgtttctc ctatgattat cgaaagattg aggcgagaag tggctacatt cagtgttcaa
     7561 aggaatggag cggttactac acgtccttca caaatgattt ggggtccaaa ggttttgctt
     7621 attgaccgct attcagcctc ggatggcgac cttttccctt atcagtttaa atattataaa
     7681 ttggggaaaa ccgttggaca gcgtacatgg ggaggtgtag tcggaattcg tggtactttg
     7741 cccattatag atggtggcat gctcaataag cccgaatttg ctccttatga tgtggaagga
     7801 aaatccttca ttattgaagg ttatggtgtt gaacccgaca tagaaatatg gaacgatccg
     7861 gctaaagaat acgagggagt agatgagcaa ttaaataagg ctatcgaggt aatactcgag
     7921 gaattgcaaa actggcccga acagttccca cctgttccac cttatccaga taaatcgaaa
     7981 taaataaata aataaatgaa acgcactgcg tgttgtaaat tgataacgcg cagtgcgttt
     8041 ttaataagct aatggaatac gtaaaacgcc tattgacaaa ttgataacca attaacaact
     8101 tataacacac aacttacaac tcacaaactc taaccgccat ccactaatca ccatccaccg
     8161 atctctcatt actgttgtgt caagtcgtca tctttggtat ttttaggttt agcgggtggg
     8221 gtggcaatgc tttcgcgcat tttcgtatct gctttcaggt tttcgaaacg gtaataatcc
     8281 atgatgccca agttgccgtt gcggaaagcc tcggcaatgg ctttagggat ttccgattct
     8341 gcttcgatga ccttagcgcg ggcttcttgt gcctttgcct tcatttcctg ttcgagtgct
     8401 actgccatag cccttctttc ttcggccttg gcttgagcga tgtttttatc ggcattggcc
     8461 tgatccatct gaagaacggc tccaatattc ttccctacat cgatatcggc aatatcgata
     8521 gaaaggattt cgaaggccgt gcctgagtcc agtccttttt gcagcaccag tttagagatg
     8581 gaatccgggt tttcgagcac gattttatgg ctttcggccg agccaatgct cgaaactatg
     8641 ccttctccta ctcgggctag tatagtttct tcgcctgcac ccccaaccag tcgcctgatg
     8701 tttgctctca cggttactct ggctttggct ataagttgaa tcccatcttt cgcaacggcc
     8761 gtaactggag gagtatcgat taccttgggc tgaaccgaca tttgtacggc ttcaaatacg
     8821 tctcttccag ccaaatcgat ggctgtggcc gtcttgatgt ccaaacccat gttggctttt
     8881 tcggcagaga tcaaagcatt gaccactgat tttacgcgtc ctccagctaa ataatgtgtt
     8941 tcaagatcat tagcattgat cttcaatcca gcttttgtgg cagcaatcaa attattaaca
     9001 atgaccgagg gaggtacttt tctccatcgc atcaaaataa gctgtatcaa agagattcta
     9061 actccgctca ccagagctga aaaccataaa cctacaggaa taaagtaaaa aataatccat
     9121 aaaccgaata gagctaccag ccctataata ataattaata gtgtactaga cataaaatgt
     9181 tgattaaatt ggttttacat aaattttatt gtattctatc tttacgacta tcaccttttg
     9241 gccaggatcg acatattctc cgaatgtatg agcttcatat tcctgacctt caatttcgat
     9301 ctttccagca ggtgccagtc gtgaaagggt taacccctgt ataccgggtt ttacgatgtc
     9361 ttcattgatg acattcacct tggccgtatt ttgctgggtt agcatgagcc gcttccatgt
     9421 attcgacttc aaagaaaaat agagcactat gaaactgaca gccaatgtaa tacctaaagt
     9481 gatcgaaccg gctatcattc catgggcagc aaaactctgc catatagcaa tgcctacaca
     9541 tgccagtgca ataatacctc ctaccgaagt tccgggaatc accagaattt cgaggaccat
     9601 aaatataagc cctactacaa ttaaaatgat gataatactc caactcatgg gattatttga
     9661 ttgcgacggt taatttacgt cggatcaatt catcgcatat aggcttaagt tcctcataac
     9721 atcccatttt tatggggcat ttccccttca agtgtgcaat agtagcgcat tgttccgctt
     9781 gttcgagggt gtgctggcaa acttccatca aacattctat cacatattca aaggtgttca
     9841 catcatcgtt gaataggatc agatccttga cccctattac ccctgttacc tcttgagcaa
     9901 tagttttttc cttggtttct tccatataat cctcccaaat cttaccggcc ataaaagtac
     9961 ggaaaaattc aatgtctcga acattttcga taaatgaagt tttatgttcg gatgacttca
    10021 ctatcgattc atgtccgagt ccggcaataa tgaagagaga aatttctgtt ctctcattat
    10081 tcctgatgaa gaagaatagg acagtatttt aaggtcgaga attcttttgt tataaagata
    10141 ccatcctgtt ctattgttta ttgaggatat tgttcgtaat tttacaaata tgtattattt
    10201 gcaacaaaag tgtattttgt gttgttaaaa ataggaatta attactctaa ggttttctgg
    10261 aataaagaaa gtaaatcgtg aaaaatttta cactcgatat tgatgagttg cagggaaggc
    10321 tttcgtatca tttacctggc tgggaagctc agaaaaatat ggcaccaacc gggagagttc
    10381 atattcctcc tgcagatatg gtgcccatcg agagtgcggt tttattgctt ttggttaaaa
    10441 actcagccga ttatgaaatt gttttcattc aacgaactga agatgatggg ccgcattcgg
    10501 gtcagatttc gtttcctggc ggacgtcgcg aacctcatga taaaacattg ctggatactg
    10561 ctttgcgcga agctaccgaa gaaattgctt taaaatgcaa tcatttgaag gttttgggtc
    10621 agcttacccc cttatacgtt cccacgagca attttatgat ttatccattt ataggatttg
    10681 ccgatggctg cgatcatttt cgacctcaac ccgacgaggt acagaaagtg atcagggttc
    10741 gactcaatga tattttggat agaaatcacc ggcgcattga agtttttact tcatcggcag
    10801 gggatagcta taaagctcca gcttatttta tagaagaggt gaggatttgg ggtgctactg
    10861 caatgattgt gagtgagttt tgcgaaataa tctcctcact cgatcgagaa aatagataaa
    10921 tttttgcctt aaatcgatta ttctgcctca aatgatgatt caataaatct tgaagtagct
    10981 aaacttttct tccagaggag cgttgttatg gtaagatatt ttatgacaaa tagttgaatt
    11041 ttgtagtgaa tttgcttttt taaatgcccc caaatgtcat agattgagga tatttctttt
    11101 gtaactgaag cagtggattg tttgccagga agaatttatg caccccgttt tttacaacat
    11161 tattttgcgt agatcgaaga aatatcgtaa ttttgcatgc gaacgaagaa ttttagaatc
    11221 atctcaagaa ttttaaagag aacgagccaa gaataccctt acgataattt tatttttgta
    11281 gttttgcccc cgaaatgggg tttgtaatca gtggagatat attcgagtaa ttttataaag
    11341 cctttacaat gcgtatctta ttagttacag ctacttggct ggaagcaaag cccttgatgg
    11401 acgggtttac ctttttacgc aagtattctg gaaacaatta ttcttttcga tacggtgagc
    11461 atcgcatcga tgtattagta acgggtatgg gcatggtagc cactgctttt ggagtaggaa
    11521 aagctctgcg aaatactcaa tacaatttgg tgctcgatgc aggaatatgc agttcttttc
    11581 gaaaggaatt ttctccgggt acggtgctca acgtaagtga ggaatttttc ccagaatttg
    11641 ggatgagtga tgatgctgga tttaagtatt tacaggacat ggggttcgat accagtttgc
    11701 ctaccgcatg caagaatact gtgttgcaga atcctcatta taatcaggta atggggttca
    11761 agaatctggc aacggctcgc ggagctacgg ttaatttggt aaatcttacg ggaaggcgtc
    11821 tcaatactta tcattccaat atagaggccg atatcgaaac tcaagaagga ggagcatttt
    11881 tctttagctg ccttgccagt gaagtgcctt ttatcgaatt aagagcagtt tcgaattata
    11941 ttggccctcc cgacgaagaa ggatgggaca tcgaaaaagc tattcaaaat ctcacctatt
    12001 ccgttcacga agccctaaat gtgcttccgc gctaaagtgt ataatttttt gatctgatag
    12061 attgattcat ttttttcatc gaaatgcaaa aaaaaattat cgttacggga gctgcaggct
    12121 ttataggaag ctgccttgtg ggtaaattga atgaattggg ttacgaaaat ctgattttgg
    12181 tagatgattt ttcctcaatt ttaaaagaac ccaattgggt aaataaaaaa tatgagatgt
    12241 gcatacccag agaagatttt ttgcaagggg ttcatgagtg ttcgtgcgat atcgaggttg
    12301 tttttcattt gggcgcccgt accgatacta ccgaaatgaa cgaaaaagta ttcgatgccc
    12361 tcaatctcac ttattccaaa cagattttta atttttgcag ccaatatcgg attcctttat
    12421 tatatgcctc gagcgcagct acttatggtg ctggagagtt gggttacagc gacgatcatc
    12481 atcttattcc tcttcttcgg ccattaaatc cttatggcat ctcgaagaac gaattcgata
    12541 aatgggttct tcagcagaac atcactcctc ctttttgggc tggttttaaa ttttttaatg
    12601 tttatgggcc taacgaatat cataaacatc ggatggcatc agtagttttt cattcggcaa
    12661 atcaaatcag gaatacaggt aaagtaaggc tgtttcgttc gcacaggcct gattatgccg
    12721 atggcatgca gaaaagagac tttgtttatg ttttcgatgt gatagaaacc ctgattcggt
    12781 tctggcaaca tagacccgtt tcgggaattt ataatctggg aacaggtaag gctgaaccat
    12841 ttctcagagt tgcggaggct gtattcgagg cacttgagtt ttcaccagct attgaattta
    12901 ttgatactcc tatagatatt cgggataaat atcagtattt tactcaggcc gatattcgca
    12961 aaattcaggc aacaggtctt actccagatt ttacttcttt gtacgatgga attatcgact
    13021 atgttgggag attcttgaaa tatcaaaaat attactgaat cggcccttcg caaatcctca
    13081 ttgggttcct taatcttcta aaatctttgg agtttctttt cgtgctttcc ttttgttttg
    13141 atagtcacga taattaagga tgatagatat gactaaagat aaaataagac cactcacggt
    13201 aatgatatct gcaacgggcg tactgaggtt tatgcctgta agtcgatgtg taatgaattg
    13261 gagataagaa tttggaaggt tttgataacc cagtaatctc aaaacatcat aatgccaatc
    13321 ggtaagcggg cagtaaccca agccatacca aatgcctgca attgtccaca ttgaagcagt
    13381 aagtaacaaa gtaatgaggt tcagcttacg cagtttagta aaacaccatc ctataagatt
    13441 gaatatcatc agagacgaat ggaaaatggt aaaaaaccag tcgagtagat gataaaagga
    13501 catagaacac cttaaagatt attgcgaagc tggataagaa attccctgat atttttcagg
    13561 gtttcgacaa tgtccatgtt tcttaggaca tggcttcgac tggttttcag gtaatcgcgt
    13621 gctcctgcaa tagtaaatcc tctttctttg agtaaatatt taattaatcg aagcgtttcc
    13681 acatcttccg tatgatagaa acgagttcct ttacggttgc gatatggatg aataccatcg
    13741 aattcctttt cccaatacct cagcgtactt ggatttacgt cgagcattct gcttacttca
    13801 ccaatcgtat aataaagatg ctgttttttt ttctgattgt tttgggaagt catgaataga
    13861 gtaagagata aagatgaaag ggaattattc acccacaggt atatttgaag attctttgta
    13921 atccgatgat tgacggataa tttcctggaa ggtagccgga tcgaggtcga acataaaata
    13981 ggccgggttt actttctggt ggttgtaaat gacttcgtag tgcaggtgtg gtccagttga
    14041 aagcccgctg gttcccacag tggcaattat atcaccgcgt tttacctttt gaccaggttt
    14101 tacattgaat gaattcagat gagcataaat cgtttgataa ccaaatccgt gttcgatgat
    14161 aatttttttg ccaaatccat ccagattgat taaagaagct tcttttacca ctccatcacc
    14221 cgtagcgtaa actttggctc ctttgggagc ggtaaagtca atgccttcat gaaacatcac
    14281 ggttttatag aaaggatgga ttctgtaacc aaaatatgaa gaaatatatt tcaaatcacg
    14341 tttgcttacc ggttgtattg caggaatagc cgtaaccata gcttcctttt tcttggccat
    14401 ttcaaagacc tcatcgaagg atttcgactg cacatagatc tggctcttta cccggtcgag
    14461 ttttttagcg gtttctaata gcaaggcaaa attatcactg ccttcgaaat tctgataacg
    14521 gtttgccccg cctattcctg cttgtcttat actggccgga actggttctg tttcgaaaat
    14581 tacgcggtag atattgtcgt cacggttttg aaggtctgtc aaaatctttt gcatctggtc
    14641 gagctcatag ttcatggctt gaacctgaac cttgtattgt tcgatctcac gttgaagaat
    14701 tctttctttt ggtgaagata aaaatttgaa ggcaagtgca ataacaacag ctgagaatac
    14761 cagacccgtt gccagaatgt aaagaattct ttttaaacga tcgcgaaatc tgacagggat
    14821 gatctgatac gttaatgaaa gtggatcaaa gtaatatcgt atttttcgtc tcataatgct
    14881 tcagattttt ttaattattg aatgatttaa aatcatcttc gatcgcaaaa ataatatttt
    14941 cacgtaaatt tgcgcccttt aaagggaaaa tttttatata taaagtattt tgaaggtttg
    15001 attcttatgg atgttcctgt tttggcacat atatttattt atgagttttt caaaagatga
    15061 tggtttaatt gaaaggaacg aattaaataa tgaaatcagc ggatatacgt aaaacttttc
    15121 tggatttttt tgctgagcga cagcatcaaa tagtgccttc ggcaccaatg gtagtcaaga
    15181 atgaccctac cttaatgttt gtcaatgcag gcatgaatca attcaaggat atttttttgg
    15241 gcaatcggcc agttgttttc ccccgtgtgg cagatacaca aaaatgcctt agagtttctg
    15301 gtaagcacaa tgatctggag gaggtgggtc atgataccta tcaccatacc atgtttgaaa
    15361 tgttgggaaa ctggtcgttt ggtgattact ttaaggaaga ggccattgca tgggcatggg
    15421 agctgcttac tgaagttttc aaaattgatg ggagccggct ttatgttacg gtttttcagg
    15481 gagatgatgc cgactattta cctcccgatc aggaatcata caagatctgg caacattatc
    15541 agactgaaga aaagattctg ttcggaggga aaaaagataa tttttgggaa atgggtgaca
    15601 ccggcccttg tggaccatgt tcggaaattc atattgacgt tcgttcggga gaagaaagga
    15661 atgcagtacc cggatatact ttggtcaata aggggcatcc taaggtaatc gagatttgga
    15721 atctggtttt catcgaattc aatcgtatgg ctaatggtca actgcagcaa cttccagccc
    15781 gccatgtcga tacaggaatg ggtctcgaac gattatgcat ggtattgcaa ggcaagcaaa
    15841 gcaactatga taccgatgtt tttcaaccta ttattcgaga aattgaaaaa ttatcgggca
    15901 agtattatgg tagcgatccc agaattgatg ttgctatgcg agtcatagcc gatcacttga
    15961 gggcggtggc ctttgcaata gccgatggtc agcttccatc caacaacaag gcaggatatg
    16021 tgattaggag aattttacga cgcgcagttc gttatggcta taattttttg ggatttcgtc
    16081 aaccatttat ggtttctttg gttcccattc tgagagatca aatgggatcg tttttccccg
    16141 aattaattgc ccatgaatca ctaattagca atgtgatggc cgaagaagaa gccaattttt
    16201 tacgaactct cgaatcggga attcaaaaat tcaattccta tatctcaaat aatcagtcga
    16261 aaggcatcat cgatggcaac tttgcctttg aactttacga tacttatggt ttcccgatcg
    16321 atctaaccca attgatggct gctgaacaag gttgggaagt agatatgcct tcgttttatc
    16381 agcacctcga agagcagaaa caacgttcgc gtgctgatgc tgcaataaat accgatgatt
    16441 ggatagaggt agtttcgggc cttggtgaaa ctactttcat aggatacgat acactggagg
    16501 ccgaagccga aatcatcaaa tatcggtatg tgtccacggc caagaaacaa tatttccaga
    16561 ttgttttcaa ccatactcct ttttatgcag aatcgggcgg acaggttggc gatcgtggat
    16621 ggataatcgc tggagatgaa aaaattgaaa tagaaaacac aatcaaagag aacaatcttg
    16681 taattcatat cgtttcacgt cttccgttgg atgtgagtca accagtgctt ttaaaggtag
    16741 atgaagtaaa acgccggctt acggctaata atcacagtgc tactcatttg cttcatgcag
    16801 ccttacgaaa ggttttagga actcatatag aacaaaaggg atcattggtg gacgaaaatc
    16861 gcttgcgctt cgacttttct cattttacac gcttaacctc cgatgaaatt ctgaagattg
    16921 aagccatggc caacggaaaa atttgggaaa acctgccatt gaaggaatgg agaaaccttc
    16981 caatggaaga agctcagaaa atgggagcca tggcactttt tggagaaaga tacggcgaaa
    17041 tggttagggt ggtggctttc gatccagagt attcggtcga attgtgtggg ggaactcatg
    17101 tgaaagcaac aggacagatt ggattgataa aaattatttc tgaaaccggt atagctgctg
    17161 gtatccgccg aatcgaagcc gttacgggca cagtagccgt ggcattttat aatcaacagc
    17221 tgcaaattct cgaagaaata aaaaccctcg taaaaaatcc tgtcgatgca ttaaagggtg
    17281 tgaagaatct tctggaagaa aaacaaaaat taactcttga agtcgaaaac ctgcgcctgt
    17341 ataaaataaa ttcgcttctg caaattcttt ctgaaaatat taaaaaggcc aatgggatca
    17401 attttttgag catagagctc gatgtggatg tacaaatggc taaagatttg cttttcgatt
    17461 taaaacggcg tttttctccc tttgtaggcc tgattgctta tataaatcaa aacaaacccg
    17521 gatttagctt gattgttagt gatgaattaa tcgagacggg gaaggtggat gccagggaac
    17581 tggtcaaagt cttgtctccc tgcatcaaag gaagtggagg aggccaagct gggtttgtaa
    17641 ctgccggagg ttcgtttaca aaaggcgtgt cagaacttct tgacaaagcc aaacaattat
    17701 tcgctcaaat cgattcgtaa tacaccatcc agaggtactg catgatagga tacatcgagt
    17761 tccttcaatg ttttttccca ttctgtacaa atccaccggg gtacgcttcc gtccatgatg
    17821 atcatctgag gatgaatatg actcaaaaat tcctgaacat gaaaggcagg attccctgaa
    17881 atgatacagt aatctggttt taccgggctt tgatctgcat aagtggtccc atcgggaata
    17941 tagtaaatca atttaccgtt ccatcttagt aaattgcttt ctgaaagaat ctgtatattt
    18001 ttgattttca gtgattgttg aatctgttga ctttcactgt atccttcgga aggagatgta
    18061 aaaagtagac catcaatacc gttgactaac catggccctt tgatgcgtgg cgaattcatt
    18121 acatacatac tctgctgcct actacgatag agtctatcgg cagtatctat gaagaaaaat
    18181 aggaaaattc cggctaatgc tgcttggaaa taagacttga tgtttttgga gaagaataga
    18241 atcagcatga taataacaaa gtagagaaca agatattgag cggctgttat gtagatacct
    18301 gatgcaaccg ctcctggcaa gctttctacc caacctacgc tctggttcat cagccagata
    18361 gagcccgaaa gaactttgcc ggcataaaat cctgcatgac caaatggtag aatcataaag
    18421 aaggcaacgc caagcatcat aataacataa gaaattggga ttaccagtag attagaaatg
    18481 atgaaatata cagggaattg atgaaaataa aataaagcga ggggaaaagt ggctatttgt
    18541 gacgcaatag atacacaaat cagtccccat attttatcgg caattttgtt tttgaaattt
    18601 agcattcgat agattccggg ttgggtaacc acaattccta ataccgccaa ataagaaagt
    18661 tgaaatccga tttcctgaat aagaaatgga ttgaaaataa gaataaagca ggccgaggca
    18721 gcaagagtat tgaagatggg cgttctccgt ttgaagattt ctcccatcga aacaaacgaa
    18781 aacatcgtag ctgctcttgt aaccgaagga cttaaaccag taataagggc gtaaatccaa
    18841 atggcaaaaa ccactataat tgacctaata agcctcccat gcggaatttt ggataaaaaa
    18901 cccagcaagt aaaacagaat caggtagata atcccaacat gtaaacctga aacggctaaa
    18961 atatgcatgg ttcccgagcc agaatatttt tgccttagtt cctggtctag ataatcacta
    19021 ttgcccaata aaatggctgc tgctacgcta tattcatctc cgctcacacc gctatttttc
    19081 atcatcttga gcaattgcat tctggccttt gcagataggg tttttatgct gaaagcatga
    19141 gcatcaccaa tgactttcca atcgttttga cttagataca attgatgata aattcctctg
    19201 cgagccagat aactcttata atcaaaagct cctggattac cagaattagc gatttcgaaa
    19261 taattgcctt tgaataatat gcgtgtaccc ggcttcaatt caccagcttc gggagttttt
    19321 tcgagataag tcatgatctt ccccgaaact ggcttccatc gagcagagtc gtataaacca
    19381 taaattttta caattacttt atacgatttt gccttggctt gaggataatc ttcaattata
    19441 gcctgaaaat attccgatgt tttgtccgat gaaaaatgtt catgtttgaa tgataattcc
    19501 ctaaggctaa ctacggatac tccagataga aacatcaaaa tatgaatcat aagtccatag
    19561 atccactgat tcttgtaaga aagccggatg tagaagaaaa gcagatatat agctgcaatt
    19621 aagaatatta ttccacataa aatgggagag aatcttattc tcgagaaata aatacttaaa
    19681 agaattcctg ttatcagagg gataaaaagc tttaatatag gattctctct tagcatggtt
    19741 tcaggttaat acatcaacga aagtatgaat tttttattta aatattcaga gggcgtaaaa
    19801 ttttgttttt aagagagata ttttttaata aatgaaaagg ttaaatttga aggaacctga
    19861 aattaaggta ttgttaatca tttatttatg aaagagaggc aaaatcacat gaacgataga
    19921 aatgaagttg ttatctttgc agcgaaaaaa ttaaaatcat ataaaaatgg ctaaagcaag
    19981 aattgcaatt aatggatttg gccgtatcgg aaggctcact ttcagagctt tgctcgaaaa
    20041 agaagaagta gaagttgtag ctataaacga cctgactgat tcaaaaacac ttgcccactt
    20101 gttgaaatac gattcagttc atcgcaggtt tcctggtaca atcgaagtgg atggtgatta
    20161 tcttgtagtt aatggaaaca agatacgcat atatgctgaa aaagacccct tgaatttgcc
    20221 atggaaagat ttgagcatcg atgtagttgt tgaatcgaca ggtgtatttc gtacacgtga
    20281 aaagatgtcg aaacatctca ctgcgggcgc taagaaagtg gctttatcgg taccagccga
    20341 caatgcggaa gatgtcgata ttaccgtggt aataggagtg aatgatcatc aaatcaggcc
    20401 tgagcataaa ctcatttcga atgcttcgtg cacgacgaac tgccttgccc ccgtggctaa
    20461 gattctgcat gaaaaattcg gtatcgtaag aggattgatg aatacagttc attcttacac
    20521 gaatgatcag gttattctgg atgcgccaca taaagattta cgaagggcac gttcggctgc
    20581 catgagtatt attcctacaa aaacaggtgc tgcaaaagct gttgcactgg ttattcctga
    20641 attaaaagga aaattagatg gatttgctat gcgagttccc acaccggatg cttctattgt
    20701 tgatttaacg gctgaactgg cggttaaaac cacgaaagaa gaaatcaata ctgccatgaa
    20761 ggacgctgca gaaggaaagt tgaagggaat attggaatat tgtattgatc cggtggtaag
    20821 cagtgatatt atcgggaaca agcattcctc tgttttcgat agcttgctca ctcaagtcat
    20881 cgaaggcaat tttgtaaaga tagttgcctg gtatgataat gaaatgggct attcgcagcg
    20941 tttggccgac cttgtcatta agatgctctg agtcggaact cgaatttggt gaataaaaat
    21001 ggccgttact tgatcaagta acggccattt tcggttttta ccatcccgat ggaaggactt
    21061 attgtcctaa ataggctttt aacaaacggc ttctcgaggt atgcttcaac tgcctgatag
    21121 ctctttccct aatctggcga accctttcgc gggtcagatt gaatttagct cctatttcat
    21181 ccaaagtaag tgcgtaattt tgcccaatgc cgtagtagag acatataatc tcacgctctt
    21241 tgggtgacaa agtcgagagc gaacgatcta tttcggcttt cagcgattca ttgatcaagt
    21301 tttcgtcggt agcaggagta tcttctgcga cgaatacatc aataaacttg gtgtcttcat
    21361 cgtcttgcag aggtgcatcc attgatacat aacgtgtgtt tactttcagc acggcatcta
    21421 ctttgctttc cgataaatcc atggcttcgg caagctcttc cgctgaaggt ggcctttcga
    21481 gtttttgctc taatcttgat gcttcctttt tgatcttatt gagcgaacct acctggttca
    21541 aaggtaacct tactatgcgc gattgctcgg ctaaagcctg aagaatgctc tgtcgaatcc
    21601 accaaacagc atacgaaata aacttgaacc ccctggtggg atcgaatttt tgcgctgcct
    21661 tgattaatcc ggcgttacct tcactaatca gatccggcaa gcttaaacct tgattctggt
    21721 actgcttggc taccgaaact acaaatctta aattggcttt gcatagcttc tccatcgcct
    21781 tctgatcgcc ttcctggatc atttgggcta atttcacttc ttcttccacc gtaatcatct
    21841 cttccttgcc aatatcctga agatatttgt caagtgatgc attctcgcgg tttgtaatcg
    21901 attttgcaat tttaagttgc ctcattgtat atatttacga attacgaatc ctaacgtaac
    21961 aattgacagt tttattggtt caatggttac atctatcctt gaaatttgtt aaaatttatt
    22021 gaaacctcta catgcgccgt ttcttagcaa atattatgcc tgaattcgtt ttcctcacct
    22081 ttgaattaaa tttttaacct cttgaaattc atttctgtat aaaaattcga tgaaaattgt
    22141 attgatttat gattcatatt tcgagtaaga aactatagct aatccctcaa agaaaggaaa
    22201 aatgaaattt gacttatatt tagtgaata
//
LOCUS       DAOORU010000018        21350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_3015_length_21350_cov_5.425687, whole genome shotgun sequence.
ACCESSION   DAOORU010000018 DAOORU010000000
VERSION     DAOORU010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21350
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3015_length_21350_cov_5.425687"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..1286)
                     /locus_tag="PLF20_02435"
     CDS             complement(261..1286)
                     /locus_tag="PLF20_02435"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HOX79899.1"
                     /translation="MNKMIDLRSDTVTRPSAEMLAYMMKAEVGDDVFGEDPTVNALEE
                     YLASMFGMESGLFTPSGTAANQIAIKIHTQPGDEVICDQTAHVYLFEAGGIAFNSGCS
                     VRLLNGDRGRFNIKQLETALNDPDDIHEPVSRLVVAENTSNRGGGSVWDYDDLREISE
                     FTGQHKLAFHLDGARLFNAITWDGTQPENYGRIFDTISICLSKGLGAPVGSVLLGNHE
                     MMKKARRIRKLFGGGMRQAGYLAAAGLFALQHNIPLLKDDLRRAQTIAKSLSQKSFID
                     SILPVESNILIFTLNKDFPSDNFLHILQQNNILALPFGSQTIRFVTHLDFNDEMLEVV
                     VNVLKAI"
     gene            complement(1279..2496)
                     /locus_tag="PLF20_02440"
     CDS             complement(1279..2496)
                     /locus_tag="PLF20_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOX79900.1"
                     /translation="MSNKLLSEREIFFRHLALTSFTPPAIQFSKAAGIYLYTPQNRIY
                     TDLSSGFSVCNIGHNHPAVVQAIHQQADLYLHQTVYGEFVQSPQVRLAETLINLLPTS
                     LDCVYLVNSGAEAIEGAMKLAKRYTGRPHLVTFRNAYHGSTQGALSITGNEILKNHYR
                     PLIPGIIQLQFNQIEDLNQIDETVAAVIIEPVQGEAGVVLPEPEFLYRLRQRCDQTNS
                     LLIFDEIQTGLGRTGSMFAFEKYKTVPDVLCLAKALGGGMPIGAFISSKKMMDTLAYD
                     PSLGHITTFGGNPVSAAAACAVLEILQNEIDISASDEKARRLIDRLENFKVINEVRFS
                     GLLIAVEFHHLNQADRALQLFWENCLISDRFLFCENALRISPPLCITFEQCHEVNKTL
                     QLIFEQLDKEFYE"
     gene            complement(2474..3556)
                     /locus_tag="PLF20_02445"
     CDS             complement(2474..3556)
                     /locus_tag="PLF20_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOX79901.1"
                     /translation="MPAFLYFCNQFFTLLHSSVQISSIINVASVAPHHHWWIITAFSV
                     VIVIMLLIDLGVFNKKTHSIGNREALTWTIVWIVVSLLFGIVVYFESGLNSASDYIAA
                     YLIEKSLSMDNIFVFILIFSYFSVPSEYHHKVLFYGIIGAIIFRAIFIFAGVELIKLT
                     YLPPFEVNGTEVQLNVVLTIFGIFLVYAGLKTFNNHKEKQKDFSKNFVVRAAHRLFPF
                     SNEYDNGKFFTRQNGKRLATPLLLVVLVIESTDILFAVDSIPAIFAISNDPFILYTSN
                     IFAILGLRALYFLLANLMPLFRFLHYGLAVILVFIGTKMLIADFFEIPSPVSLAAVVA
                     ILTISILLSVFIKEEKISTNVEQTSK"
     gene            3598..4407
                     /locus_tag="PLF20_02450"
     CDS             3598..4407
                     /locus_tag="PLF20_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX79902.1"
                     /translation="MELKVVVITGASSGIGRALAQEYGERGWHLALLARNFDKLLQLK
                     DELSGKASSILCLSTDVSKEEECKNAMDEIIATYGRIDVLINNAGISMRALFKDVDLN
                     VIRRLMDVNFWGTVYCTKYALSSLLANKGSVIGIVSIAAYVGLPGRTGYSASKFAIRG
                     FLETLRTENLRNGLHVLIAAPGFTATNIRSTALLADGSQQGQSPRNEGKMMTTTQVAK
                     KIVKAIDKRKKTLILTFWEGKFTVFLQKFFPSLVERLTYLHMAKEPDSPFK"
     gene            4485..4970
                     /locus_tag="PLF20_02455"
     CDS             4485..4970
                     /locus_tag="PLF20_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79903.1"
                     /translation="MKIEIKTEILPLRGLPLINFGDTIEDVESILGKPEETLMLEDIE
                     ERTLPAMVYLYWNSCLTFFFSTLEEKPFLIMIETDDLNAQLLDEKIFKMKPKQLIALL
                     SGQKLFDFEKEQERHEIRYTWDEWNIDFYFDGDKLSTVNWSAQLDDTGHVVIPEEPFK
                     W"
     gene            complement(4982..6802)
                     /locus_tag="PLF20_02460"
     CDS             complement(4982..6802)
                     /locus_tag="PLF20_02460"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOX79904.1"
                     /translation="MIREPNFQYPFLIGTIFLFAYLIVRIILWYIELSKYDKLRFIHF
                     WKTKSVWAALKDAIADGLLHSSIFKKNLLLGYMHMSLAFGWFLLIVIGHLEVMVDRRS
                     LNFPFYYAVFYRYYQPHPDFAGARVFGALMDLLLLIILSGVALAILKRIKKQIFGMKR
                     TTKLKPFDKIALTSLWLIFPLRLLAESLSAAYYHNGSFMSNTLGAWFIQNVRVSAIYD
                     LSWWLYSCSIGFFFIALPFSRYMHIPTEIVFIFLRHAGIMPKKYWNSYAQIQMYSCSR
                     CGICLDACQLYEAGVSNVQSVYFLQYIRNNENVDKSLFGCLICGRCQEACPVHINLND
                     LRTALRIKASKPLTFSYGYTQMALQQKTDIIYFAGCMTHLTPPIKFSMARIFNFLGEK
                     VWYMDEDKSICCGGPLMLAGQYDSASQLIQKNTELIMASGAKTLILSCPICYKAFRED
                     YVLPGISIKFHAVYLNDMIRQGRLPKINLGFKTIYHDPCALGRGSKIYDEPRYVIDSY
                     SQRMRLKKEKAEALCCGAGLGGINLPIETKNKVTFEAMTYFARFKPNVLITACPLCKK
                     TFARDRRIPVIDIAELTARAIDEYVNEQKKSVREARLIES"
     gene            complement(6799..7137)
                     /locus_tag="PLF20_02465"
     CDS             complement(6799..7137)
                     /locus_tag="PLF20_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOX79905.1"
                     /translation="MSTFDFLKVRRLYIDYDNLDQELLNEVIREEPSFQWCISCGCCT
                     ATCSAGNHTTFNIRRISLILRRGEIEGLEEELKKCMLCGKCTLVCPRDIQLRNVVMNI
                     KKIVLKKKTA"
     gene            complement(7144..8169)
                     /locus_tag="PLF20_02470"
     CDS             complement(7144..8169)
                     /locus_tag="PLF20_02470"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOX79906.1"
                     /translation="MKKIAVIGGGIAGLEVSRLLLQEGLPVTLIEKGSRLGGNLRNYA
                     HLFPDFTPSDEILGNYRFLPSNSDLQLLMNTQIESIDFSTQPYTLHSKDGIAIPADPL
                     IIATGYQVFDAKLKEEFGYGIYDGVITSVELEKMIKQHHIVTPYGKIPRRIAFIHCVG
                     SRDAKVGNSYCSRLCCITAIKQAIETKKLLPETEIFCFYIDLRMYGLTFEELYLNAQK
                     EYNIQFVRGRLSETSEMIDKRLIIKAEDTLIGRPLQMKADMLVLMVGMEASDFSKQFA
                     YHNGLEIGDHGFFQPLNQFNARSKSQKEGIFFAGTSIGPMSVAETIEHARAAALSAIS
                     YLKDKEY"
     gene            complement(8311..9375)
                     /locus_tag="PLF20_02475"
     CDS             complement(8311..9375)
                     /locus_tag="PLF20_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HOX79907.1"
                     /translation="MAQNYTEPSKKIYWKDYVKNIPDDDYFFFRSCIRQTFFPGAEQA
                     FLFILKNHLHKNYFDDPRLTTCTGIGYHSDIIPLETLHTLIARHFSIMNESGYSNLII
                     SCVTTFGVMTEVLNTWHEFPETLEKTRKNLLKTTGRTFELPKAIAHASDIIYLYKDEI
                     GKQLVYQLVNKETSEPLKVVDHVGCHYSKIFPHHGIGGAEFPKVLSEIIKAWGGEVID
                     YPERRHCCGFGFRQYLIKESRGYSLSHAQIKFESMQPYHPDFILTNCPGCNMFMDRWQ
                     YVIQETAGKVYSSDGNGIPVLSYEELAALLLGYDPIQIGLFMHQTEVLPLLEKLGIQF
                     DKNEYSKLRAESHHNTKISR"
     gene            complement(9377..10027)
                     /locus_tag="PLF20_02480"
     CDS             complement(9377..10027)
                     /locus_tag="PLF20_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOX79908.1"
                     /translation="MGKDIQMIPMEEIYADPRIRENMNACINCGVCTALCPAADYYDY
                     EPRYVERIILMRNADALEELMSSDYIWYCGQCMSCKARCPRQNAPGMVVSALRYWSQK
                     LGLFVRSPKGRQQLIIKQNIGRNIYDLGYCVHPSRLSLEEHPEQGPVWQWILDNIEKT
                     YALVNANIDKDGPGPMRKIPDEALQEIRSVLQGTGAIEFWKQIETKVLEYNNDDKR"
     gene            complement(11208..12071)
                     /locus_tag="PLF20_02485"
     CDS             complement(11208..12071)
                     /locus_tag="PLF20_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008625412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOX79909.1"
                     /translation="MRQLKITKQVTNRETASLDKYLQEIGKVELLTAEEEVALAQRIK
                     QGDQVALEKLTKANLRFVVSVSKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNKIGSLNKINKAYAKLEQTFEREPNYEEIAQ
                     QLEITENEVKDSLRNSGRHISMDAPLVEDEDNTMLDVLRADDTPSPEAGLIYESLCHE
                     IQRVISTLSPREADVIRLYFGLEGTHPLTLEEIGEKFDLTRERVRQIKEKAIRRLKHN
                     SRSKILKSYLG"
     gene            complement(12135..14273)
                     /gene="pnp"
                     /locus_tag="PLF20_02490"
     CDS             complement(12135..14273)
                     /gene="pnp"
                     /locus_tag="PLF20_02490"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOX79910.1"
                     /translation="MQGITQNIFLPDGREVVMETGKLAKQADGAVVVRMGNTMLLATV
                     VSTKEAKEDTDFLPLTVEYQEKYAATGRFPGGFFKREARPSEYEILTARLVDRALRPL
                     FPDDYHAETQVLITLISGDKNFIPDCLAGLAASAALSVSDIPFNGPISEVKVARVDGK
                     FIVNPVVNDLERSDMDLMVAATLDNIVMVEGEMKEISENELLQALEYAHEVIKVQCLA
                     QIELSKKVGACQNKRQYNHEVNDEELKQRVHDFCYDKIYDLASHPTNKHERTEKFEQI
                     LNDFIETIPVEERTGKELMVKRYYYRTQWEAVRNVILERRVRLDGRQLDEIRPIWSEV
                     DYLPTAHGSAIFARGETQSLTTCTLGTKLDEQTIDGAVLEGKVNFILHYNFPPFSTGE
                     VRTTRGTSRREIGHGNLALRALKPVMPLPEENPYTIRVVSDILESNGSSSMATVCAGT
                     LALMDAGVKIRKPVSGIAMGLITDEQTGNYAILSDILGDEDHLGDMDFKVAGTEVGIT
                     ACQMDIKVSGLSAEILNQALVQARKGRLHILREMRKTISSPRPDYKPHVPRIVQMTIP
                     REFIGAVIGPGGKIIQDIQHETNSTIVIEEVGEFGIIDIVAVDRDSIEAVKKKIKSII
                     AIPEIGEVYTGKVKTIVPFGAFVEILPGKDGLLHISEIDWKRINDIESVLKVGDEVTV
                     KLIDIDRTTGKLKLSRKALLPKPGNNNTKQ"
     gene            complement(14460..14729)
                     /gene="rpsO"
                     /locus_tag="PLF20_02495"
     CDS             complement(14460..14729)
                     /gene="rpsO"
                     /locus_tag="PLF20_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOX79911.1"
                     /translation="MYLTSETKKQIFKEYGGSEINTGSTEAQVALLTHRINHLTQHLK
                     SNPKDLVTQRSLVLMVGRRRHLLNYLRRLDIERYREVIKKLGIRK"
     gene            14956..17061
                     /locus_tag="PLF20_02500"
     CDS             14956..17061
                     /locus_tag="PLF20_02500"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOX79912.1"
                     /translation="MKNNFIMICVMLLTIVACKNEKQRTNPLLAKWDTPFEVPPFDRI
                     DTSDYFPAMMESIRQHEVEIEAIAENNSAPTFENTILAFDTSGKLLTRITNVFFNLLE
                     ANTNDQMQKIAEKISPILSEYQDNIYMNPKLFDRIKAVYDERQQMGLDDQQKRVTEKY
                     YNDFVRSGASLDSTHQTRLKEINQELSLLSLKYGNNVLAETNNFKLIIDNKEGIEGLP
                     QEVIDAAAEAAKVAGLKDKWVFTLAKPSMIPFLQYSERRDLREKIYRAYFMRGDNNNE
                     YDNKKIIADMVKLRAEKAELLGYDNWASYVIAENMAKTPIAVDEFLMNLWNAALPVAK
                     NEASQMQAIIHKEGGNFELEPWDWWYYAEKICREKYNIDENEIKPYFSLQNVRQGMFD
                     VANKLYGITLSRRTDLPMYHKDVEAYEVKDADGSHLGILYLDYYPRAEKRVGAWCTEF
                     RSAGWENGQRVSPVVSIVYNITPSTASTPSLLNWDETTTLFHEFGHALHALFAEGKYR
                     RTAGIVPRDFVELPSQIMENWAQEPEVLKIYAKHYQTGEIIPNTLVQKIEKSGQFNQG
                     FETVEYLAAAILDMKYHELASQNANIEPNTFEKKSMDEIGLIKEIIPRYRSTYFQHIF
                     SGGYSAGYYVYIWAEVLDADAFDAFVKSGDVFNKELASKFRNYCLAHVGEDDAMTQYV
                     KFRGQQPSIGPLLKRRGLK"
     gene            complement(17611..19251)
                     /gene="pruA"
                     /locus_tag="PLF20_02505"
     CDS             complement(17611..19251)
                     /gene="pruA"
                     /locus_tag="PLF20_02505"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOX79913.1"
                     /translation="MNNAIFMYDLPKNEPILGYLPCSLERKELEKEIQRLSSEVIEIP
                     LIIGGKEIYTGNLGEVVMPHNHSHVLGTYHKAGEKEVKMAIDAALEAHSYWTNVSWVE
                     RMSVTLKIAELISKKYRALLNAATMLGQSKNIYQAEIDCVCETIDFLRFNAYYISEVY
                     NHQPFSESGCINRLEYRPLEGFIFTVSPFNFTSIASNLNMSPVLMGNTVVWKPASTAI
                     LSNYVLMKIYKEAGLPDGVINFLPGQGSFIGNIVLNHRDLAGVHFTGSTATFNEFWKT
                     ISHNIGHYRSYPKLVGETGGKDFVFLHSSADIDEAATALIRGAFEYQGQKCSAASRAY
                     VPRSIWPSLKDKMLGMLHEIKIGDPTNYENFVNAVIDEASFNKIMHYIDYAKGSKEAE
                     IIWGGKGDKSVGYFVDPTIIFTSNPHFLTMEEEIFGPVLTVYIYEDDQFDETLRLCDQ
                     TSPYALTGAVFSKDREALNHACRVLRYAAGNFYFNDKPTGAVVGQQPFGGSRASGTND
                     KAGGPINLIRWTSVRTIKENLNPPIDYRYPYMVNKECK"
     gene            complement(19483..19674)
                     /locus_tag="PLF20_02510"
     CDS             complement(19483..19674)
                     /locus_tag="PLF20_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOX79914.1"
                     /translation="MSTKKVFPLRISPEMLDTLQQWANDEFRSINGQIEYILYEALSK
                     ACRLKSSKTQPTDPPEDEF"
     gene            complement(19729..20244)
                     /locus_tag="PLF20_02515"
     CDS             complement(19729..20244)
                     /locus_tag="PLF20_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79915.1"
                     /translation="MRRFEEIQVLRHIALGWVLMAAIIILFFAGIIQPILFFFDPDRG
                     NLSITDFIITLSTSLIVISLILFLFLTMQLKVKIDEKGISIEYLPFLKHKEFRWDDIQ
                     QAYVKKYHPIKDYGGWGYRGFGKKKAYNLSGSWGIQIMMKNGKQILIGTKQPEKVRQV
                     LQHYGFENISE"
     gene            complement(20257..21126)
                     /locus_tag="PLF20_02520"
     CDS             complement(20257..21126)
                     /locus_tag="PLF20_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008764407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOX79916.1"
                     /translation="MNREKFVIPLSGYLGVVVTILTLALGILLFVFFPNSVLAIVIGI
                     ILCLLTIFMVVGLLVVNPNESAVLVLFGKYSGTVVKNGFFWVNPFYVRKKISLRARNL
                     NADPIKVNDKLGNPIMIGIVLVWKVKDTYKAAFEVDDYLQFVDIQSEAAIRKLAGHYP
                     YDNFDDEQRETTLRSGGEEVNHVLEQELTERLQIAGIQVIEARISYLAYSSEIASAML
                     QRQQATAIVAARHKIVEGAVSMVEMALDMLSERQVVTLDNERKAAMVTNLMVVLTSDK
                     AASPVVNAGTLYH"
BASE COUNT         6405 a         4282 c         4183 g         6480 t
ORIGIN      
        1 tataaaaaat gtttaggaac ttttagaatt ttgtcggtta atagtgaaaa aatatgataa
       61 tcataacgaa atcgaagtaa ataattatcc aattttatgc caacaatcac atgtatattt
      121 tctaaattat tgatttagaa tatttagcaa aatatcattc ctatgttcga tttttatgat
      181 aattccaata atgaattatt ccgaaatgaa atgaaaagat aatactgaaa gcggatagtt
      241 tcgcaagttt taactttatt ctaaattgct ttcaagacgt tgaccacgac ctcgagcatt
      301 tcgtcattga aatcgagatg ggtaacgaag cggatagttt gtgagccaaa gggcaaagcc
      361 agaatattgt tttgctgaag aatatggagg aaattatcgg aaggaaaatc tttgtttaaa
      421 gtgaagatca ggatattact ttcaacgggc agaatagagt cgataaaaga tttttgagaa
      481 agtgatttag ctatagtttg tgctcgacgc aggtcgtctt taagaagagg aatgttatgt
      541 tgaagagcaa acagtcccgc agcggctaaa tagccagcct gacgcatgcc tcctccaaag
      601 agtttacgaa tgcggcgtgc ctttttcatc atttcatggt taccgagtaa aaccgagcct
      661 actggcgctc ctaaaccttt cgaaaggcaa atgctgatgg tatcgaaaat tctgccataa
      721 ttttcgggtt gagttccatc ccaagttatg gcattaaaaa gacgagcacc atcgagatga
      781 aacgcaagct tatgctgacc agtgaattcc gaaatttctc ttaaatcatc ataatcccat
      841 accgaacccc ctcctctatt gcttgtgttt tcggctacaa ccagtcgtga aacaggttca
      901 tgaatatcat cggggtcatt cagggcagtt tctaattgct ttatgttaaa ccttcccctg
      961 tccccattta acaaacgaac cgagcaaccc gaattgaagg cgattccacc tgcttcgaag
     1021 agataaacat gggcggtttg gtcacaaatt acttcatcgc caggctgagt gtgtatcttg
     1081 atggcaatct gattggctgc ggttcctgaa ggagtaaaaa ggccagactc cataccaaac
     1141 atgctggcta aatattcttc caaggcattt accgtgggat cttctccgaa aacatcatca
     1201 cctacttcgg ctttcatcat ataggcaagc atctcggccg aaggtcgtgt taccgtatca
     1261 ctgcgaaggt cgatcatctt attcataaaa ttctttatct aattgttcaa aaataagctg
     1321 taaggtttta tttacctcat ggcattgttc gaaagtgatg caaagaggtg gagaaatacg
     1381 cagagcattc tcgcaaaata aaaaacggtc gctaatcaaa caattttccc agaaaagctg
     1441 caaagctcta tcggcttggt tcagatgatg aaactccact gcaatgagta aacccgaaaa
     1501 tcggacttcg ttgatgacct taaaattttc gagcctgtcg attaatcgtc tggctttttc
     1561 gtcggacgca gagatatcta tttcattttg caaaatttcg agaactgcgc atgctgctgc
     1621 agcgctaacg ggatttcctc caaaagtagt aatatgcccc agagaaggat cgtaagccag
     1681 cgtatccatc atttttttgg atgaaatgaa ggctccgatg ggcattcctc caccaagggc
     1741 tttggccaga cacaacacat cgggaacggt tttgtatttt tcgaaggcaa acatgcttcc
     1801 cgtgcgtccc agtcccgttt gtatctcgtc gaaaatcaac aaactatttg tttgatcgca
     1861 acgctgccga agtcgatata aaaactcggg ttcgggaagt acaactcctg cttcgccttg
     1921 cacgggttct attattaccg ccgctactgt ttcgtcaatt tgattcaaat cttctatctg
     1981 attgaactgt aattgtataa taccaggaat aagcggtctg taatgatttt tcaggatttc
     2041 gtttcctgtt atgcttagtg ctccctgagt gcttccgtga taagcatttc gaaaagtaac
     2101 gaggtgaggt cgaccggtat aacgtttcgc cagtttcatg gctccttcta tggcctcggc
     2161 tcccgagttt acaagataga cgcaatcgag cgaagttgga agcagattta tgagtgtttc
     2221 ggctaacctc acctgaggcg attgcacaaa ttctccatag acggtttgat gaagatataa
     2281 atcagcttgt tggtggatag cttgtaccac tgcaggatga ttatgcccaa tattgcaaac
     2341 cgaaaaacca ctgctaagat cggtatatat ccgattttga ggagtatata aataaatacc
     2401 ggcggctttc gaaaattgta tagcaggagg tgtaaaggaa gttagcgcca aatggcgaaa
     2461 aaatatttcc ctttcactta gaagtttgtt cgacattggt tgaaattttt tcttctttga
     2521 taaatacaga gagaagaata gaaattgtga gtatggccac tactgcagca agagacacag
     2581 gcgagggtat ttcgaaaaaa tcggcaatca acattttggt acctataaat accaaaataa
     2641 ctgccaatcc ataatgaaga aagcgaaaca aaggcattag attggccaga aggaaatata
     2701 aggccctcaa tccaagtatg gcaaatatat ttgatgtata aagaataaaa ggatcgttac
     2761 taatagcaaa aatggcaggg attgaatcta cggcaaataa aatatcggta ctctcgataa
     2821 ccaaaacgac taataataaa ggcgtagcaa gtcgtttccc attttgtctg gtaaaaaatt
     2881 tcccgttgtc gtattcattc gaaaaaggga ataaacgatg ggctgcccta acaacgaaat
     2941 tcttcgaaaa atctttttgt ttttccttat gattattgaa ggtttttaat cctgcataaa
     3001 ccagaaaaat tccaaaaatc gtaagtacga cattgagttg aacctcagtt ccatttactt
     3061 caaagggagg cagataggta agcttgatca attcaacgcc agcaaagata aatatggctc
     3121 taaatatgat ggcaccaatg attccataaa aaagtacctt gtggtgatat tctgatggaa
     3181 cactaaaata actgaatatt aagataaaaa cgaaaatatt atccatcgaa agcgactttt
     3241 cgatgaggta ggcggcaatg taatcgctcg cactgttgag ccctgattcg aaatacacca
     3301 ctatcccaaa aagcagcgaa accacaatcc agacaattgt ccatgtaagt gcttcacggt
     3361 taccaatgga atgagttttt ttattgaaga ctcccaaatc aatgaggagc atgatgacaa
     3421 ttacgacact gaaagccgta attatccacc aatgatgatg aggagcaaca gaggcaacat
     3481 ttattataga agaaatttgc acggacgaat gaagaagggt aaaaaattgg ttgcaaaagt
     3541 aaagaaatgc aggcaaaaca ctaattcatt attttcgcat atttattttc gaaaaaaatg
     3601 gaattgaaag ttgtagtgat tactggagct tcgtcgggca ttggcagggc cttagctcag
     3661 gagtatggag aaagaggatg gcatttggct ttattagcac gcaacttcga taaattactc
     3721 caacttaaag atgaactttc cggaaaagct tccagcattc tttgtttatc aaccgacgta
     3781 agtaaggaag aagagtgcaa gaatgccatg gatgaaatta ttgcgactta tggaagaata
     3841 gatgttttga ttaacaatgc tggcatctcc atgcgtgctt tattcaaaga tgtcgatttg
     3901 aatgtgattc ggcgattgat ggatgttaat ttctggggaa cggtttactg cactaaatat
     3961 gccttgtctt cacttttagc aaacaagggg agcgtgatag gcatagtatc cattgcggct
     4021 tatgtgggct tgcccggccg aacgggttat tcggcttcga aatttgccat tcggggattt
     4081 ttagaaaccc tccggacaga aaatcttcgt aatggacttc atgtactgat agccgcacct
     4141 ggttttactg ccaccaatat acgctccacc gctctgctgg ccgatggaag ccaacaggga
     4201 caatctccac gcaacgaggg aaagatgatg actaccactc aggtagccaa aaaaatagtc
     4261 aaagccattg acaaacgaaa gaaaacattg attcttacct tctgggaagg aaaatttacg
     4321 gtttttcttc agaaattctt tccttcactg gtcgagcgtt taacttattt gcacatggcc
     4381 aaggaacccg attcgccttt taaataactt atcatgattt tttcagttct tttaaggtgt
     4441 attaataatt gaacttcgtg aatggtataa aattttatcc caatatgaaa atagaaataa
     4501 aaacggaaat cctgcccttg aggggattgc ccctgattaa ttttggcgat acaatagaag
     4561 atgtggaatc tattcttgga aaacccgaag aaactttgat gctcgaagac attgaagaaa
     4621 ggacactccc tgcaatggtc tatttatatt ggaatagttg tttgactttc tttttcagca
     4681 ccttagagga gaaacccttt ttaataatga ttgaaacaga tgatttgaat gcgcaattgc
     4741 tcgatgagaa aattttcaaa atgaaaccca agcagttgat tgccctatta tcgggccaga
     4801 aacttttcga ttttgagaaa gaacaggaaa gacatgaaat ccgctatacc tgggatgaat
     4861 ggaacatcga cttttatttt gatggggata aactgtcaac agtaaactgg tcggcacaat
     4921 tagatgatac tggccatgta gttattccag aagaaccttt caaatggtag atgccaaaga
     4981 tttaagattc tatcaaacga gcttctctga ctgatttctt ttgttcgttc acatattcat
     5041 cgatagctcg agctgtgagt tcggcgatat ctattactgg aatacggcga tcgcgggcga
     5101 aggttttttt gcataaaggg caagccgtga taagtacatt gggtttaaat cttgcaaaat
     5161 aagtcattgc ttcgaaagta actttatttt tagtctctat aggtaggttt atccctccta
     5221 aaccagctcc acaacataat gcctcggctt tttctttttt aagacgcatt ctttgagaat
     5281 aagaatcgat cacataacgc ggttcatcat agattttact tcctcttcct aaggcacagg
     5341 gatcatgata aatggtttta aaacctaaat taatttttgg taaccgtccc tgacgtatca
     5401 tgtcattcag atagactgcg tgaaacttga tggagatacc cggcaaaaca taatcttctc
     5461 tgaaggcctt gtaacaaata gggcatgaaa gtattagggt tttggctcca gaagccataa
     5521 ttaactcggt gtttttctga atcaattgcg aagccgaatc atattgaccc gccaacatca
     5581 aggggcctcc acaacagatg gacttgtcct cgtccatata ccagactttt tctcctaaaa
     5641 aattaaaaat tcgggccatg gaaaatttaa tcggaggcgt aaggtgagtc atacatccag
     5701 caaaataaat gatatcggtc ttttgttgca aggccatttg agtatatccg taagaaaaag
     5761 taagcggttt gctggcttta atgcgaagag ctgttctcaa atcattaagg ttgatatgaa
     5821 ctggacaggc ttcctgacat cggccacaaa ttaggcaccc aaatagagat ttgtcgacat
     5881 tttcattgtt tctgatatat tgaaggaaat aaaccgactg tacattcgat actccggctt
     5941 cgtataactg gcaggcatcc aaacaaatac cacacctcga acaggaatac atctggatct
     6001 gtgcataact gttccagtat tttttgggca taattccggc atgacgaagg aaaatgaata
     6061 cgatttcggt agggatatgc atgtagcgcg aaaatggaag tgctatgaaa aagaatccta
     6121 tcgagcatga ataaagccac caactgagat cgtaaatagc cgaaactctt acattttgaa
     6181 taaaccatgc acctaaagta ttgctcatga agctgccatt atgataatag gctgcactca
     6241 ggctttcagc caataaacga agaggaaata tgagccatag agaagttagt gcaatcttgt
     6301 cgaatggttt gagcttagtt gtccttttca tgccgaatat ctgtttcttg atacgcttga
     6361 ggatggctaa tgcaactccc gataaaatta tgagaagtaa taaatccatc agagcgccga
     6421 agaccctagc cccggcaaag tcgggatgtg gttgataata ccggtaaaat accgcataat
     6481 aaaaagggaa gtttaaacta cggcggtcaa ccatcacttc gagatgtccg atcacaatca
     6541 gtaaaaacca tccaaaagcc aggctcatgt gcatgtatcc gagtaataga tttttcttga
     6601 aaatgcttga atgcaataat ccgtccgcaa tggcatcctt caatgctgcc caaaccgatt
     6661 tggttttcca gaaatgaatg aagcgtagct tgtcgtattt agatagttcg atataccaaa
     6721 gaatgattct cacaatcaaa tacgcgaaca agaatatcgt ccctataaga aatggatatt
     6781 gaaaattggg ttctctaatc atgctgtttt cttttttaag acgatttttt tgatattcat
     6841 tactacattt ctcagttgaa tatctcttgg acataccaga gtgcatttgc cacagagcat
     6901 gcattttttc agttcctctt cgagtccctc aatttcacct cgtcgtaaaa tgaggctaat
     6961 acggcgaatg ttaaaggtag tatgattgcc ggcactgcat gttgcggtgc agcatccaca
     7021 actgatgcac cactgaaagg aaggttcttc gcgtattact tcgttcaaaa gttcctgatc
     7081 caaattatcg taatctatat ataaacgcct tacctttaaa aaatcgaatg ttgacatttg
     7141 tttttaatat tctttgtctt ttaaatacga aatcgctgat aaagctgctg ctcgggcatg
     7201 ttcaatagtt tctgctaccg acatgggtcc aatgcttgtt cctgcaaaaa aaattccttc
     7261 tttttggctt ttgctccttg cattgaattg attaagaggt tggaagaaac catgatcacc
     7321 tatctccaat ccattatgat aggcaaactg tttcgaaaaa tcggaagctt ccatgccaac
     7381 catgagtacg agcatatcgg ccttcatctg taaaggacgc ccaataagtg tatcttcggc
     7441 tttaattatc aaccgcttat ctatcatttc cgaagtttct gacaatcgac cccttacaaa
     7501 ctgaatatta tattcttttt gtgcatttag atatagttct tcaaaggtaa gtccatacat
     7561 tcttaggtcg atataaaaac aaaatatctc ggtttctgga agtaatttct tggtttctat
     7621 ggcttgcttg atcgccgtaa tgcagcaaag acgtgaacag tatgaatttc ccacctttgc
     7681 gtcacgcgaa cctacacaat gaataaaagc tatacgtctg gggatctttc cataaggagt
     7741 tacaatatgg tgctgtttga tcattttttc cagttctacc gaggtaatta ctccatcata
     7801 gataccgtat ccaaattctt ccttcagttt tgcatcgaaa acttgataac cggtggcgat
     7861 gataagaggg tcggcaggaa tggctattcc gtcttttgaa tgcaaggtat agggctgtgt
     7921 cgaaaaatct atgctttcta tttgggtatt cataagaagt tgaagatcag aattacttgg
     7981 caggaagcgg taatttccca atatttcgtc agaaggtgta aaatcaggaa agagatgagc
     8041 ataatttcta aggttgccgc ccaatcgtga acctttttct atcagggtaa ccggtaaacc
     8101 ttcctgcaag agaagtctcg aaacttccag ccctgctatc cctccgccta ttacagctat
     8161 ttttttcatc tgtcgtttat gaaatttgtt ttccttctaa tttactgaaa tatttttcta
     8221 gttgaaaaat gaacttcatt aattatcgtt ggcttgtttt tcatggcgtc atcatttttt
     8281 cggaacgata ttgggattat cttgatggat ttatcgggaa attttcgtat tatgatggct
     8341 ttcggctctc agttttgaat attcattctt atcgaactga atgcccaatt tttccagcaa
     8401 gggcaagacc tcggtctggt gcatgaaaag gccgatctgg ataggatcat aaccaagcaa
     8461 taatgcagcc agttcttcat agctcaaaac aggtatgcca ttcccgtcgg atgaataaac
     8521 cttaccagca gtttcctgaa tcacatattg ccagcggtcc atgaacatgt tgcagccagg
     8581 gcaattggta aggatgaaat cgggatgata gggttgcatg ctttcaaatt taatctgagc
     8641 gtgagagaga gaatagcctc tgctttcctt tattaagtat tgcctaaaac caaagccgca
     8701 acagtgcctg cgttcaggat aatcgatcac ttcgccgccc caggctttta ttatttccga
     8761 taaaaccttc gggaattcgg cacctcctat gccatgatgc ggaaaaatct ttgaataatg
     8821 gcaaccaacg tggtcgacaa cctttaatgg ttcgctcgtt tctttgttta cgagttggta
     8881 aaccaattgt ttgcctatct catctttgta aagataaata atatcgctgg catgagctat
     8941 ggcctttggt agttcgaaag ttcttccagt agttttcagg agattctttc gagttttttc
     9001 gagcgtctcg ggaaattcat gccaggtatt caaaacttcc gtcattactc caaaagtggt
     9061 aacgcacgag atgatgagat tggaatagcc cgattcgttc ataatggaga aatggcgcgc
     9121 tatgagtgta tgcaacgtct ccagaggaat aatatcgctg tgatagccaa tgcccgtgca
     9181 agtggtaagg cgaggatcat cgaaataatt cttatgaaga tggttcttca aaataaaaag
     9241 aaaagcctgt tcggctcccg gaaaaaatgt ctgacgaata caactgcgga agaagaaata
     9301 atcgtcatcg ggaatgttct ttacataatc cttccagtaa atcttcttac ttggttctgt
     9361 ataattttgt gccatattac cgcttatcgt cattgttata ttctaaaact ttcgtttcga
     9421 tttgcttcca gaattcgatt gcgcctgttc cctggaggac agaacgaatt tcttgtaagg
     9481 cttcgtctgg aatttttcgc atggggcctg gtccatcctt gtcgatattt gcatttacaa
     9541 gagcataagt tttttcgata ttgtcgagaa tccattgcca caccgggcct tgttcgggat
     9601 gttcttcaag agaaagccgc gaaggatgaa cacaataacc cagatcataa atgtttcgcc
     9661 cgatattttg cttgatgatc aattgttgcc tgcctttggg gcttcgcaca aaaagtccga
     9721 gtttttgcga ccaatatcgc aaagcactta ccaccattcc tggagcattc tggcggggac
     9781 agcgtgcctt gcacgacata cactgcccac aataccagat atagtccgac gacatgagct
     9841 cttccagagc atcggcattt cgcatcaaaa tgatacgttc cacatatctg ggttcataat
     9901 cataataatc ggcggcagga cacagcgctg tgcaaacccc acaattgatg caggcattca
     9961 tgttttccct tatccttggg tctgcgtaaa tttcttccat tggaatcatt tgtatatcct
    10021 ttcccattgg gttgaatcat tcttcgaatt aatcctatac aaaattccga ttttcttgtg
    10081 agaaatcgaa caaattcatc ttaacttttg tagattaata aattacccaa ttaaaagtca
    10141 attcctaaat aacgttgcct gtttttaatt cgattttttt accaaaaatg gaagtgctca
    10201 aaagaaagta tcactttgag gaggctttct tcatacgatg aatcaaaatc gaatcatcac
    10261 atcagtttag agctttatct tgaatatctc ataaaagaag cagctcttct atgaataatg
    10321 agagctgcct ttcagatttt gcttctctcg agaagcacag tgcaaaaatc agccactgtt
    10381 aagtgtgttt ttaccacatc atccaaaatg gggattctat ggttttttgt tgctacaaat
    10441 ttaatcaaat atcagtgaaa acaacctctt tcagcaaaat tctcttgtta ataaaagtta
    10501 attggataat caagtaattt atcggatagc accagaatat attaaaacat cgtaaaatga
    10561 tattagcctt ccgaaaagac aggagtttga attcactcga agcgtcattt atcgaaaaga
    10621 taatgggcca tacagaaatc tctttcttat ttatcaatat ttaatattgt taatcagcgt
    10681 attggatgag agtatgaatt ttgtccccta attcatggtt ttgaattaaa gaaaagatgt
    10741 aactttgcaa aaattttatg aactgaccgt gattatatcg ttgttattga ataactgcaa
    10801 attaacggta gtgattatcg ataattgata taaaaaatgg aataggaata gaaatggaca
    10861 attcgatgga attaagcaaa tttttgattt tctgaggaag ctaagggaaa cggcttttgg
    10921 gccacaagag gaaaaacgaa ggacctataa taattgaaga aactaaacaa ataattgaag
    10981 aaactaaaca gaagattgat ttaatggaaa taaactaatg gttgtatgga taggatagtt
    11041 tgattttaat ggttaattca tttggatttt ggttgattac ttggattttg gttgattgat
    11101 tgaatggcag cgggcttcat ggagagcccg ctgtttatta aacaaaaatc ctaaaaggca
    11161 gggaattgca ttaactttca ggattcctca aactggattg ttgtttctta ccccagataa
    11221 gatttcagta ttttgctacg gctgttatgt ttaagccgtc ggattgcctt ttccttgatc
    11281 tggcggacgc gttcgcgagt caggtcaaat ttttcgccaa tttcttcgag ggtaagtggg
    11341 tgcgttcctt ccagtccaaa ataaaggcga atcacatctg cctctctggg tgaaagggtt
    11401 gaaattactc tttgaatttc atgacataga gattcataaa ttagacctgc ttcagggctg
    11461 ggggtatcat cggccctaag aacatccagc atagtattgt cttcgtcttc taccagaggg
    11521 gcatccatcg aaatatggcg gccagaattt cgcaatgaat cctttacttc gttttcggtt
    11581 atctccaatt gctgagcgat ttcctcataa ttgggttctc tttcgaaagt ttgttctaac
    11641 ttggcataag ccttatttat tttattcaac gaacctatct tgttgagagg taggcgcaca
    11701 atacgtgatt gttcggcaag tgcctgaaga atactttgcc tgatccacca taccgcataa
    11761 gagataaatt taaaaccccg ggtttcatca aaacgctggg ctgccttgat caaacccaaa
    11821 ttaccttcat taatcagatc aggaaggctt aaaccttggt tttgatactg tttggaaaca
    11881 gatactacaa aacggagatt ggctttggtt aatttttcga gtgctacctg atcgccttgt
    11941 ttaatccgct gcgccaaagc aacttcttct tcggctgtaa gtagttcaac tttcccaatt
    12001 tcttgcaaat acttgtcgag tgatgcagtt tctcggttag taacctgttt ggtaattttt
    12061 agctgcctca tattaggatt tttttaccat gtttcatttt acgacttatc cctataaagg
    12121 tttcaaaaat tcaattattg tttggtattg ttattccctg gttttggcag gagagccttt
    12181 cgcgagagtt ttaattttcc tgttgttcgg tcgatatcga tgagtttcac ggtaacttcg
    12241 tctccaactt tcagaacact ctctatgtca ttaattcgtt tccaatcaat ttctgatata
    12301 tgtagcaagc catccttacc cggaagtatt tcgacaaaag cgccaaaagg cacaatggtt
    12361 ttcactttgc ccgtatatac ttcaccgatt tctggaatag caatgatgga tttgattttc
    12421 tttttaacag cttcgatcga gtcgcgatcg acggctacaa tatcaataat tccaaattca
    12481 cctacttctt ctattactat tgtagaatta gtttcatgtt gaatgtcctg aattatcttt
    12541 cctcctggac caattactgc accaatgaat tctcttggaa tagtcatttg aacgatacgt
    12601 ggtacatggg gtttataatc gggccgtgga gacgaaatag ttttgcgcat ctcccgaaga
    12661 atatgcaaac gcccttttcg agcctgtacg agtgcctgat tcagtatctc tgccgataaa
    12721 cccgatactt ttatatccat ttggcaggct gtaatgccaa cttcagtccc agcaacctta
    12781 aaatccatgt ctcctagatg atcttcatct ccgagaatat cggaaagaat ggcataatta
    12841 cccgtttgtt cgtcggtaat taatcccata gcgatacccg acactggttt tcttattttt
    12901 acaccagcat ccatcaaagc gagggttcct gcgcaaacag tagccataga ggatgaaccg
    12961 ttcgattcca agatatctga taccactctt atggtatagg ggttctcctc aggtaatggc
    13021 atcacaggtt taagtgctct cagtgcaagg ttgccatgcc caatttctct acggctggtt
    13081 cctcgggtcg ttcttacttc tcctgtcgaa aagggtggaa aattataatg aagaataaaa
    13141 ttgaccttcc cttccagaac tgcgccgtca atagtttgct cgtcgagctt ggtcccaagt
    13201 gtacaagtgg ttagcgactg cgtttcgcct ctcgcaaata tggctgaacc atgtgccgta
    13261 ggcagataat ctacttcaga ccatataggc cttatttcat ccaattgcct tccatcgagg
    13321 cgtactctcc tttctaatat aacattacga actgcttccc attgggtacg ataataatac
    13381 cgtttcacca tcaattcctt accagttctt tcttccacgg ggatggtttc gataaaatca
    13441 ttcagtattt gttcgaattt ttccgtacgt tcgtgtttat tagtaggatg agaagccagg
    13501 tcataaattt tatcatagca aaaatcgtgt actctttgct taagttcctc atcatttact
    13561 tcgtggttat attggcgctt gttttgacaa gcaccaactt ttttcgatag ttcgatttga
    13621 gcaaggcact gcactttaat tacttcatga gcatattcaa gagcctgaag tagttcattt
    13681 tccgaaattt ccttcatctc gccttcaacc atcacgatat tatcgagagt agcagctacc
    13741 ataaggtcca tgtcggaacg ttctaaatcg tttacgacgg gattcacgat aaatttgcca
    13801 tctactcttg ctaccttcac ttccgaaata ggaccattga aagggatatc agaaaccgaa
    13861 agagctgcgg atgcagccag gccggcaaga cagtctggga taaaattctt atcacccgaa
    13921 atcaaagtta tcagtacttg agtttcagcg tgataatcat cagggaataa aggccttaga
    13981 gcacggtcta ctaagcgggc tgtgagtatt tcgtattctg atggtctagc ttcccgttta
    14041 aagaatcctc caggaaaacg tcctgtggca gcatattttt cttgatactc cactgtcaaa
    14101 ggcaaaaaat ccgtatcttc ttttgcttct ttcgtagaaa ccactgttgc cagaagcatt
    14161 gtattaccca tacgaactac cacggctcca tcagcttgct tggctaattt ccccgtctcc
    14221 atcacaactt cacggccgtc gggaagaaat atattttgtg taattccttg cattttttat
    14281 cttctttttt aaaatgaata acttaaaaat attcgacatc atgcagtttg atgtggatac
    14341 gtgaagaatt aattataatc atcattagat gtcctctgtg ttgagtatca gcaagctcct
    14401 aaagtaatga agaacaatcc gtatgaatta ccttcaatta tgcaatattg caaatcaatt
    14461 tattttctaa ttccaagttt ctttatgact tccctgtatc tttcaatatc caggcggcgc
    14521 aaataattca gcaggtgtct tctgcggcca accatcaata ctaatgaccg ttgcgtaacc
    14581 aaatcttttg gattggattt cagatgttgc gtcaaatgat tgatcctgtg cgttaaaaga
    14641 gcaacctgag cctccgtaga accggtattt atctcagaac caccatactc cttgaaaatc
    14701 tgtttcttgg tttcagacgt taaatacata tacttttcct tatttttgca ttcaatttta
    14761 ggggtgcaaa tatacaacat tttccttcag tatgattttt caaaagcttt ttaccaaaat
    14821 ttcgctgcat tccattcccg tacgatattt cttaaatcat cttactttta taacaaattt
    14881 taaacttctc gatgaaaatt cattcatcct ttgggattca tttagttatc atatcaatct
    14941 tcaattaaaa tcactatgaa aaataatttt atcatgattt gtgtgatgtt acttaccatt
    15001 gtagcatgca aaaacgagaa acaaagaacc aatcctttgt tggcaaaatg ggacacacct
    15061 ttcgaagtcc ctccttttga tcgaatcgat acctcagact atttccctgc gatgatggaa
    15121 agtattcggc agcacgaagt cgaaatcgag gccattgccg aaaataattc tgctccaacg
    15181 ttcgagaata ccatattggc attcgatact tcgggtaagc ttcttactcg aattaccaat
    15241 gtatttttta atttactcga agcaaatacc aatgaccaga tgcaaaagat tgcagaaaaa
    15301 atttcaccaa ttctttcgga atatcaagac aatatttata tgaatcctaa actctttgac
    15361 aggataaagg ctgtatacga tgaaaggcaa caaatggggc tcgatgatca acaaaaaaga
    15421 gttaccgaaa aatattataa tgatttcgtc agaagcggtg catctctcga ttcgactcat
    15481 cagaccagac tgaaagaaat caatcaggaa ctttcgctgc tttcgctgaa atacggtaat
    15541 aacgttttag ccgaaaccaa taatttcaaa ctgataatcg ataataaaga gggcattgaa
    15601 gggttgcctc aggaagtaat cgatgcagca gcagaagcag caaaagttgc agggctgaag
    15661 gacaaatggg tatttaccct tgccaaaccc agcatgatac cttttctgca atattcagaa
    15721 cgtagggatc tgagggagaa aatttatcgt gcctacttta tgcggggtga taataataat
    15781 gaatacgata acaaaaaaat tattgctgat atggtgaagc tccgggctga aaaagccgag
    15841 ctgctggggt atgataattg ggcatcttat gtaattgccg aaaacatggc caaaactccc
    15901 attgctgtag atgaattcct aatgaatcta tggaatgccg ctctacccgt ggccaagaac
    15961 gaagcctcac aaatgcaagc gatcatccac aaggaaggag gaaactttga acttgagccg
    16021 tgggattggt ggtattatgc cgaaaaaata tgcagagaga aatacaatat agatgaaaat
    16081 gagataaaac cttatttttc acttcaaaat gtgcgtcagg gaatgttcga tgtagccaat
    16141 aaactttatg ggattactct ttcccgcaga acggacctgc caatgtatca taaggacgtt
    16201 gaggcctatg aagtgaaaga tgccgatggc agtcatttgg gtattcttta tttggattat
    16261 tatccaaggg ctgaaaaacg cgtaggcgca tggtgtacgg aatttcgttc ggcaggctgg
    16321 gaaaacggac agagagtaag tcccgtggtt tccatcgttt acaacattac tccttccaca
    16381 gcctccaccc catcattact caactgggat gaaactacca cgcttttcca tgaatttgga
    16441 catgcattgc atgcactttt cgctgaaggg aaataccgac gaactgccgg aatagttcct
    16501 cgcgactttg tcgaactccc ttctcagatc atggaaaatt gggctcagga acctgaagta
    16561 ctgaaaatat atgcaaaaca ttatcaaact ggggaaataa tccctaacac actcgtccag
    16621 aaaatcgaaa aaagcggcca atttaaccag ggttttgaaa ccgtagaata tcttgcagca
    16681 gctatactcg acatgaaata tcatgaattg gcatctcaaa acgccaacat agagcctaat
    16741 actttcgaaa agaaatcaat ggacgaaatc ggtttaatca aggaaataat cccccgatac
    16801 agaagcactt attttcaaca tatctttagc ggtggctatt ctgctggtta ttatgtgtat
    16861 atctgggctg aagttctgga tgccgatgct ttcgatgcat tcgtcaaaag cggagatgtt
    16921 ttcaataagg aattggcttc gaagttccgc aattattgtc tggcacatgt tggcgaagac
    16981 gacgccatga cacagtatgt gaaattccgc gggcagcaac cttccatcgg gcctttactg
    17041 aaaagaagag gtttaaaata aaaatcaatt ccatatttat ttagccttat tgaaacccgt
    17101 aaatcattac gggttttttt tattttttcg aagcaacttc tattcttatc agatctatcc
    17161 cacatacgaa agagacagaa aagcggcaat ggataaatat agaaccttgt atttcaattg
    17221 aattataaat tttcagaagc tgattttaat attattgtca gaacaagaca gtgatgccca
    17281 tttcttcaaa taactacatt attttacctt gcgtataaaa caccataagt cagaggagag
    17341 attaattatg cccgacgatt actaacttag atttatttta ttattaagat ggaggatgga
    17401 tgggaaagat caggctttta ttgctgtatc atctgaagac cagaaaaact acctgaccga
    17461 taaccatttt gccctttacg agttcttatt taatctggca gtggaatttt tctcattcat
    17521 ccattgaatt gcggctgagc cggagcctca gccgcaataa aagaactctt ctccagtaat
    17581 aaaaatatga tttcgaatcg aaggcattta ctacttgcac tccttattta ccatataggg
    17641 atatctataa tcaatgggag gattgagatt ttctttgata gtacgaacac tggtccagcg
    17701 tatcagattg atgggtcctc cggccttatc gttcgtaccg ctggccctag agccgccaaa
    17761 aggctgttga cctactaccg cacccgttgg tttgtcgttg aaatagaaat tacctgctgc
    17821 atatcgaagg acgcggcaag catggttcag ggcttctctg tctttcgaaa acactgcacc
    17881 cgtgagagca taaggtgaag tttggtcaca aaggcgaagt gtttcatcga attgatcgtc
    17941 ttcgtaaata taaaccgtga gcacgggtcc aaaaatttct tcttccatgg tcaagaaatg
    18001 aggattggag gtgaagataa tggtaggatc gacaaaataa ccaacggatt tatctccctt
    18061 tcctccccag attatttcgg cttctttaga acctttggcg tagtcgatgt aatgcattat
    18121 cttattgaat gaggcttcat cgattactgc attcacgaaa ttttcgtaat tcgtggggtc
    18181 gcctattttg atttcatgga gcattccaag catcttatct tttaaacttg gccatatcga
    18241 tcgcggtaca taagcccgtg aagccgccga acatttttgt ccctgatact cgaaagcgcc
    18301 tcttatgagg gccgtggcag cttcatcaat gtcggcactc gaatgaagaa atacgaagtc
    18361 tttccctccg gtttccccca ctagcttggg ataggagcgg taatgaccaa tattgtggct
    18421 aatggttttc cagaattcat tgaaagtggc agtggaacct gtaaaatgaa ccccagccag
    18481 atcgcggtga ttcaagacga tgttgcctat aaatgaacct tgtccgggca gaaagttgat
    18541 gactccatcg ggaaggccgg cttctttgta aatcttcatt aacacataat tcgaaagaat
    18601 tgcagtggag gcaggtttcc aaactaccgt gttccccatc aaaaccggcg acatgttcaa
    18661 attggaagct atggaagtaa aattaaatgg cgacactgta aagataaatc cttctaatgg
    18721 cctgtattcc aatcggttaa tacaaccgct ttcggaaaaa ggctgatgat tgtaaacttc
    18781 tgaaatataa taggcattaa accgcaaaaa atcaatggtt tcgcaaacac aatcaatctc
    18841 agcctgataa atattcttac tttgtccaag catggtagca gcatttaaga gggcacgata
    18901 ttttttcgaa atcaattccg ctattttcag cgtaacactc atcctttcta cccaagaaac
    18961 gttcgtccaa taactatgtg cctcaagagc agcatctatg gccatcttta cttctttttc
    19021 tcctgcctta tgataggtgc ccaatacatg agaatggttg tgtggcatga ctacctcgcc
    19081 caaattccct gtataaattt ctttcccacc tataataagc ggaatttcaa ttacttcact
    19141 tgataatcgc tggatttcct tctccagttc ctttcgctcg agtgaacagg gtaaataccc
    19201 aagaattggc tcattcttag gcaaatcata cataaaaatt gcgttgttca taaaatttat
    19261 tatttatttt ttcgacttgc aaagatattc aactcgcttt taaaaaacaa acatactcag
    19321 aacttttccc tcattttatt cccaaattga ataaaagaaa attttattcg ttatgggtca
    19381 cttcatcact ctgagtagaa aattcaggat ggaaagagat tttgaattta tgattctcct
    19441 atttatcgta ataacttggc acaacgtatt tttcctgatt ctttaaaact catcttcagg
    19501 ggggtcagta ggctgagttt tacttgattt taacctgcac gccttcgaaa gcgcttcgta
    19561 gagtatatat tctatctgac cgttgatgct tctaaattca tcattagccc attgctgaag
    19621 tgtatcgagc atttcgggcg aaattctcaa tgggaaaacc tttttcgtgc tcatgttttc
    19681 gagtgaatat ttagaacgat tatcttatgt ttattcgatg aattttattt attcggatat
    19741 attttcaaat ccataatgtt gaagtacttg cctgactttt tcgggctgct tggttccaat
    19801 gagaatttgt ttcccgtttt tcatcataat ttgtatcccc cagctaccac ttaaattata
    19861 ggcctttttc ttcccaaaac cccgatatcc ccaaccgcca taatccttaa tgggatggta
    19921 tttcttcaca taagcttgct gaatatcatc ccatcgaaat tctttatgtt ttaagaatgg
    19981 gagatattca atggagattc ccttttcgtc gatttttact ttcaattgca tggtcaaaaa
    20041 caaaaataga ataaggctga tgacgattaa gcttgtacta agggttatga tgaagtcggt
    20101 gattgacaaa ttacctcgat cgggatcgaa aaaaaagaga atgggctgaa taattcctgc
    20161 gaagaatagg atgataattg cagccataag tacccagccc aaagcaatat gacgcaaaac
    20221 ctgaatttct tcgaatcttc tcattatcag agaggattaa tgatagaggg ttccggcatt
    20281 tacgacggga gaagctgctt tatcggaggt aagcactacc atgaggttgg ttaccatagc
    20341 ggcttttcgt tcgttgtcga gagtaactac ctgacgttca gagagcatat cgagcgccat
    20401 ttcgaccatg ctaacggctc cttccacaat tttatggcga gcagctacta tagccgtggc
    20461 ttgctgacgc tgcaacatcg cactggctat ctccgacgaa tacgctaaat aactgatgcg
    20521 ggcttcaata acctgtatcc cagctatctg aaggcgttcg gtgagctcct gctccaaaac
    20581 atgatttacc tcctctccgc ccgagcgtaa agtggtttca cgctgttcat catcaaaatt
    20641 atcataggga taatgcccag ctagcttccg aatggctgct tcactctgaa tatctacaaa
    20701 ctgcaggtaa tcatccacct cgaatgccgc cttgtaggtg tcctttactt tccaaaccag
    20761 tacaattcct atcatgatgg gatttccaag tttgtcgttc actttgatgg gatccgcatt
    20821 caaattacgc gccctgagcg aaatcttctt gcgcacataa aagggattaa cccaaaaaaa
    20881 accattcttt acaaccgttc ccgaatattt gccaaacaga accaaaacgg ccgactcatt
    20941 aggatttact acgagcaatc ctactaccat aaaaatagta agtaggcaaa gtatgattcc
    21001 aattactata gcaagtacgc tattaggaaa gaaaacgaat agtagtatgc ccaaagcgag
    21061 tgttagtatg gtaacaacta ctcctaaata tcccgaaagg ggaataacga atttttctcg
    21121 attcataatg aaatgatatt aaaatgatat tgcaataata ctaactattt cgaaattttg
    21181 aacgatttat aaaaaaaaat ttttagctta taatgcaata atttatattg caatatttta
    21241 gcctttgaat gtcaaaacga cagcatgttt tttcctatcc atgtgtttaa gtgtatctat
    21301 atttttacca ccctcatgct tttctctcct tggtttccag ttactttgaa
//
LOCUS       DAOORU010000019        20706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_3136_length_20706_cov_5.399739, whole genome shotgun sequence.
ACCESSION   DAOORU010000019 DAOORU010000000
VERSION     DAOORU010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20706
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3136_length_20706_cov_5.399739"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PLF20_02525"
     CDS             complement(<1..283)
                     /locus_tag="PLF20_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOX79917.1"
                     /translation="MQNTSNKTLHLISFDMPYPPNYGGIIDVFYKIRALHRVGVNIIL
                     HVFDHGKASSDELKEYCQQIYYYERKTGIDSQLSTLPYIVKSRRQPDLLK"
     gene            complement(264..1388)
                     /locus_tag="PLF20_02530"
     CDS             complement(264..1388)
                     /locus_tag="PLF20_02530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX79918.1"
                     /translation="MAKKRVIVSVINDLSTDQRVNRSCGLLFEMGYEVWLVGRILPHS
                     PPLPSHPWKMHRMHLLFRRGALFYAEFNLRLFLYLIFHPADLLLANDLDTLLANYLIH
                     LLRKTPLVYDSHEFFTETPEVIHRPLVRGIWLTIERSILPRLEIMITVNQSLADIFHH
                     KYKIPVVSSRNVPELKPLEVKTDRTSLSLPDDKNILILQGSGINIHRGAEELIASLRF
                     LDNVLLLIIGGGDVMPQLKIMANNLHVNDKIQFFDRMPAEELQNYTMMADIGLSIDKD
                     ICPNYHFSLPNKLFDYIHAGLPVLASPLPEIRKIVEHYQVGRIIHGHEPEILANDIQS
                     MLDDKAALNFYRHNCLKARDKLNWENEKQILIPIYAKYLE"
     gene            complement(1454..2926)
                     /gene="proS"
                     /locus_tag="PLF20_02535"
     CDS             complement(1454..2926)
                     /gene="proS"
                     /locus_tag="PLF20_02535"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOX79919.1"
                     /translation="MAKDFTKRDENYSQWYNELVIKADLAENSAVRGCMVIKPYGYAI
                     WEKMQQALDKMFKDTGHVNAYFPLFIPKSFFSREASHVEGFAKECAVVTHYRLKNDPE
                     GKGIVVDPEAKLEEELIVRPTSETIIWDTYRGWIQSYRDLPLLINQWANVVRWEMRTR
                     LFLRTAEFLWQEGHTAHETREEAFAEARRMLDVYADFAENFMAVPVLKGVKSPSERFA
                     GAEETFCIEALMQDGKALQAGTSHFLGQNFAKAFEVQFLNRENNLEYVWATSWGVSTR
                     LMGALIMSHSDDNGLVLPPKLAPYQVIIVPIYKKPEQLEQISHKAHQIRESLLKLGIS
                     VKYDDSDNVTPGWKFNEYEFKGFPVRIAIGPRDLENQTVEIARRDTLEKSIIPMDGLE
                     LKIKDLLDDIQKNLYHRALKFREENTFTVDTWEDFCERIEKGGFVLAHWDGTTETEEK
                     IKEATKATIRLIPLNPQAEAGKCIYSGKTSPYRVVFAKAY"
     gene            3057..4778
                     /locus_tag="PLF20_02540"
     CDS             3057..4778
                     /locus_tag="PLF20_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79920.1"
                     /translation="MKKIFRLLPLMTVVWGLAACSSAYLANTPFDEAYDVQRSNITSQ
                     SAKTTPSIAQTTPSAIELQQQKNSLNPSEEESDFSSAEQQYSTRIAEGGEIVSPPDRI
                     LYNEYYDENDEFDFDDYYDYAYSVRIKRFHRPVYGAGYYDDCYTNLYWYTYDPWVWGM
                     SVYLGYDWWWWDWYGGPYFSLGYNWGWGSINWHWGFPYSGWYYYPHFFYHYPWYPWYY
                     GWGAYPYYIGWWNGFWFGHYYNSHDPNSYYYGRRVSRHETNGAASGGTFGEYYENYVN
                     NQRRGGQGTNGVTQPSGQGSESSLTQRRIQAAGVNQQAVKDKPGEEKNLTQGTLNERR
                     ISASESTQVTPKTDQPAVTPARIRSGEENLAAPKPSNEQSEGNVSNRRISGNVNQTAE
                     QSQTQDSRLRQTQENYAKPQASPANPQQYDYNRYRRTSPATNPEQLARPKTQGNQNDR
                     PVRTYTPPTYSRPRSSNEYTTPRRITIPQQSEQPSQMNQGVKRETQKSSERNKSYTPP
                     SSSRPNYSVPSSSNSHQNNSSRPSYNSSSSQERHSSSSFSSPSRSSSNSGGGSSSSSR
                     SGSGRRR"
     gene            4867..6312
                     /locus_tag="PLF20_02545"
     CDS             4867..6312
                     /locus_tag="PLF20_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79921.1"
                     /translation="MRKISLMGILLFATVMVFAQNATDALRYSSVNYFGTTRFMTMGG
                     AFGAVGADFSVLSTNPAGLGLYKSSEVCFTPLIYHGQTKSSFWANKAEDDRYNFAFSN
                     MGAVLTFEFPSQLETPSWRYVQFGFGVNRLLDFHNRIMVEGFNNDNSLLTGYVEYANR
                     YGIGKNSPEELAERANLIFYDEATNRYYCDMPDGGVLQRKSISTEGSMNEVVLSAAAN
                     YSDKLYVGLTFGFPYFRYYESSLYSENDVENRNEFFRSFTLRQQLETTSTGFNLKAGV
                     IVRPVDWLRLGVAYHSPMFFTDMNDVWDSYIESYFDKEVPNKSVGSPLGEFEYELITP
                     MKVIGSVAFQVGKIGLISADYQFVDYRDARFHPTGEFADINDIIQSSYSTQNIIRVGT
                     EWRFGPFSVRGGYGFYSSPFNNNINDGERKSGSLGVGYRSDNFFVDLGFVQTKSSEKY
                     FLYDPMNGDPLPAANTDFTWNSLLMTLGFKF"
     gene            complement(6381..6758)
                     /locus_tag="PLF20_02550"
     CDS             complement(6381..6758)
                     /locus_tag="PLF20_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOX79922.1"
                     /translation="MKRIISSKEAPKAIGPYSQANEANGFLFISGQIPIDSSRGQLVE
                     GGIKEQTHQVMRNIGAILKEASLDYKDVVKCTCLLSNMEHFGEMNEVYAQYFTTDPPA
                     RAAYGVVKLPLGVLVEIECIAAR"
     gene            complement(6888..8825)
                     /locus_tag="PLF20_02555"
     CDS             complement(6888..8825)
                     /locus_tag="PLF20_02555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HOX79923.1"
                     /translation="MHEKKNRILAAILLGIFAIIILLIAFLSPGIGGEGDSFTHYFIA
                     RHAASDPKWLLDLWGRPLFTLLATPFAQFGYSGMKIFSILMALITAWLGYLMADKMKF
                     QHPWLAIPFIILTPLYFNQIFSPLTELAMAMFLMAGIYTYISKKYILSAILFSLLPFI
                     RLEAFVILPVVAIAFLVDRKYPHILLLLTGYLIFSCIGYFLFKDFLWIYHKNPYQSGA
                     TEIYGKGSLLHFIRHTDKIQGYLMASLFLLGLIGWGLQIIKKKGELYLGENHLGVYII
                     TAGSFLIYYAAHSYSWWSGKGNSLGLIRVIAAVLPAASIISHAGYTLLINYLKKFKIP
                     PAITAVLVLILLVRTTLVYPYPIRPYAEEKVIAEAAEWIKENGLANKKLYYYHNLFAL
                     FLNKDPMNDDEGKMMIHFTSIKPDQIPVGALLAWDAHFGPNEGGTPLENILTDSSFKL
                     LKAFEPETPFKVLGGHDFKVYLFEKTSQTQFPVFFDTNSGQIFFMRLGFEGHDAFDKW
                     HSQEESFEGEFSLKMESSDEFTEILNTSCSSIPFQDSLAIIEIKAWLKETSSNSINTT
                     RLVIHATKDNHQLYLPLFPDTLSIQKDNWKPIQGKVSLKKFLPDGHLKIYFWKTNPNE
                     TAFIDNVSIILPKNEDKIHVE"
     gene            complement(9022..10314)
                     /locus_tag="PLF20_02560"
     CDS             complement(9022..10314)
                     /locus_tag="PLF20_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79924.1"
                     /translation="MKKTLFLGATLMLLVFSSYAQDKVLEKSGKKPKWVNGLAKDYII
                     ASGTGADIQSAQQSALTSIKEQIVSAVAENVKSTSTMYKEEANYNNNVNLFLEKYAST
                     TNTQSGKVPFLQGISLSKAEEYYWEKLQRPNKTITYIYHIKYPFSEFELKKLVSDFRL
                     YDRKLTFQLESLLEQIDSVQTVEQIEGNMAQLASLKDYFMDARQDQCNLGISQYRSLL
                     NSIELVELENKLGELKYCLRLGNRPISTSKKPIVKSECARIGSVINNADNVIIKYDYE
                     NCYEDPENNILVKYRFGTTDVQKQFYFDVAANKTSIFVSDPLHFKTILKENEMVTKSI
                     LDLTIHSKYDSPFTIEKVVLEWKGLAPIIIDNIQQNFKGKGSHNLRLNINQPININET
                     SALGKTLPVVSGYIYYRSSATGELMSYRIYNQSYTTDW"
     gene            complement(10420..10998)
                     /locus_tag="PLF20_02565"
     CDS             complement(10420..10998)
                     /locus_tag="PLF20_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79925.1"
                     /translation="MKMKSTILVAGAFLFAVTLAGCKSSKKTVDSTFGKEVIVPCSDE
                     EFHTDSKYFRGTGNGISIDLSTARNKAKMDANTNLAQSINTTLKSVSERYVNERQFDA
                     SSEFEQKFEQLTREVVNQQLNNVEVACYKTTQMKDGKFQVFTAVQVSKDEVLNNIANR
                     VSKDKKLQVDYDKMKFEQVFNEEMKKLESERP"
     gene            complement(11068..12039)
                     /locus_tag="PLF20_02570"
     CDS             complement(11068..12039)
                     /locus_tag="PLF20_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79926.1"
                     /translation="MKKRIIFFIIAFGFIYNLNAQNYLGKTDDLGRLAIAAIVPNQAE
                     GIPLSAHQLLVNKMEQIVVQNGLGATPVNPRFCMIPLINVLEKEITPTAPPMQALTLD
                     ITFYIVDALSQNIFSQTSIQVKGVGQTEDKAYIQAIRGINVKAGQFKGFIERGKEKII
                     EFYNSQCDVVLKNAQALAGQKQYEEALFVLLSVPDVCRECYDKCMDLSIDIYKQYADY
                     KCTEYLSGARASWANMDVDRAAEYLGKITPDMQCYKEASQLADQITQKQIADGANVWQ
                     FKMKKYDDSIEKDKMMIQAVKEVAKSWAYWGAAPYFNWDWSWIYIKK"
     gene            complement(12136..13095)
                     /locus_tag="PLF20_02575"
     CDS             complement(12136..13095)
                     /locus_tag="PLF20_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016270588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6175 family protein"
                     /protein_id="HOX79927.1"
                     /translation="MKKLTFTFIFILSVLTGFGQAKKPVLMVVPSDQYCISRGYKMVF
                     QNQGMTQTLPDYKAALQSDPDLRLVITKMGQIMADRGFPLKDLEQELKNLEQERAESS
                     MLTSSSSGSEMAESPIDALKRVAKADIILDLDFDIKRQGPQKYIVFNLKGLDAYTAKQ
                     VTGVAGAGNPSTAASPELLLEEAVLSHMDNFNAGLMRHFDDMFANGREVKVMVKVWAN
                     WGQNLESEFGPDSKELSEIIEDWFFDNCVQGRFNLSDASENFMKFEQVRIPMMRTDER
                     GRERAVDTRSFVSDLRKYLNELGVPSKLYLRGLGEAWLILGEK"
     gene            complement(13111..13629)
                     /locus_tag="PLF20_02580"
     CDS             complement(13111..13629)
                     /locus_tag="PLF20_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79928.1"
                     /translation="MKTKSLTLSLVLVSLMIVTASAQSRKERKILYSSQYNYEIEPVG
                     VGQDGTKVFKVWGYGKDVNAAVMNAKEAAVAACIFKGLPGGAGSAPTPPICSQPNAEV
                     TYADYFDEFFKIGGKYLQYIAVTNDGDPAAEDRIKLKKGYKVAIYVQVMYDALRKQLE
                     ADGIARRLDSGF"
     gene            complement(13654..15324)
                     /locus_tag="PLF20_02585"
     CDS             complement(13654..15324)
                     /locus_tag="PLF20_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79929.1"
                     /translation="MNSKLLFVLATSLVPLIGVQAQSIDQAKVIPSTYDRSSLTCLYM
                     KFPGENHVTEIASKFQQVVFSDKYYNNNLANVIFEAPYSRTNTEIVPEVAIKDYLTNQ
                     KIAKSVISKWYNRKEDGTMTMDLIFERGMFNASDAEYIKAQTTKRGNALLQDYGNRLI
                     QRSYILVFDFANVKNMSEAKVADQHGWEATVTAYLYKIDFNEEIQAALYDCWIYPEDS
                     PEVKAEKLQKFEQLEIPIEFVTKTTHSLSASQANPDTQLGKLTKQKSDDELLMELVQS
                     GYDETLYYLERKYEDFMVKATIYKVKPIQVKIGKKEGLKCDHRYFVYEYLYDEKTNTV
                     KPVYRGVIRATSKIADNRQLATGEMPTSTFYQTAGRKLQTGYLVRQQNDIGIEILAGY
                     EMGEIGGPYGRLDFRLGRFIGLKAFFIYLEGGGQQKEYEYHYPSYTKATTEDVTFIHY
                     GVGLAQGLMLMRNMELRPYIGIGLESASSDEIDKADKGNLSTLFLKFGGNLAINLRHN
                     IQLTGGINYYALIGNASNKDKSDLGIKWDEIFQDRKGLSGLVGLKIMF"
     gene            complement(15977..17494)
                     /locus_tag="PLF20_02590"
     CDS             complement(15977..17494)
                     /locus_tag="PLF20_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HOX79930.1"
                     /translation="MSPTLVFGILAVYFAILMLVSALTSKNTSNATFFTGNHKSPWIL
                     VAYGMIGASISGVSLISVPGEVGNNGFSYFQFVLGYPLGYLFIALILMPIYYRLGLIS
                     IYSYLQQRFGNVSYHTGATLFFISQSLGASLRLYLAASVLQMAFFDAFGIPFFVTVII
                     ILALIWAYTYRSGIRTIVWTDTLQTTLMLAAVFFSVIAIGKEMNLDLPGLTKTIAKSD
                     YSKIFNWDWRSSQNFFKQFLSGAFICIVMTGLDQNMMQKNLTCPNIKDAQKNMITYSF
                     ILIPVNLIFLSLGALLFLYINSKGLNFHDSSGFYFDKASGIYHNTDQLYPLLALKFFP
                     KIIGISFLMGIVAAALSSADSAITALTTSFCVDFLKIKGEESHTTRRWVHLGVSMLML
                     AIIMVFKALNDKTVINAIYTIAGYTYGPLLGIFMFGLYTSRKLKDNVSPYIAILIPTL
                     CYLISSNSVKWLGGYKFGYELLLLNGLLTFISLWIFSEPGNKTRNLNTVKWNSSK"
     gene            complement(17501..18670)
                     /locus_tag="PLF20_02595"
     CDS             complement(17501..18670)
                     /locus_tag="PLF20_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009038820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HOX79931.1"
                     /translation="MPSKIVFFLFLLPIILLSRSVNGQNIIIPGAWKTDSYFSSLGGD
                     RAIGVVANASSMIGEKHLIDSMISAGINVKKIFAPEHGFRGEAEAGEFIQNTIDEKTG
                     LPIISLYGNQKKPSSKEMSGLDVIVFDIQDIGVRFFTYISTLHYMMESCAKEGIPLLV
                     LDRPNPNGDYIDGPVLDLSFRSFVGMNPIPIVYGMTIGELARMINGEGWLRNGMKCEL
                     TIIPLDGYTHSTPVILDREPSPNLPNTQSIRLYPSLCLFEATDISVGRGTDFPFQVIG
                     FPAPIFGNFTFVPKAIPGKSIHPLHKGDTCYGIDLRRDSLHTRFTLKFILDFYHLSNW
                     GEKFFKRPDFFDKLAGSSLLRSQILDGLDENQIRESWQPQLQEFMLKRKPYLLYP"
     gene            18997..19191
                     /gene="rpsU"
                     /locus_tag="PLF20_02600"
     CDS             18997..19191
                     /gene="rpsU"
                     /locus_tag="PLF20_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006045671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOX79932.1"
                     /translation="MIIVPVKEGDSIDRALKKFKRKFEKTGVMRELRERQKYTKPSIR
                     RREEKTRAIFIQKLRDQELI"
     gene            19280..20167
                     /locus_tag="PLF20_02605"
     CDS             19280..20167
                     /locus_tag="PLF20_02605"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOX79933.1"
                     /translation="MIESFITYLEREKRYSKYTCTSYSIDLQQFESYCQLQYGHSDID
                     TVSSDVIRSYVVWLIEKDYSHRSIHRKIISLRQYYRYLIRLGYIHANPARDVPVPKIP
                     ERIPMFVSEENMKNVGNVASKRKNDYETIRDKLIIEMLYATGMRVSELIHLRICDINL
                     EGMNIKVMGKRSKERLIPIGEKLADHLRQYLEVLRRFGFVASPESWLFSTRKGQKIYP
                     LLVYRVVNEFLSKVTTLEKRSPHILRHSFATQLLNAGADLNAIKELLGHSSLASTQIY
                     THSDFETLKKIYQQSHPKA"
     gene            20184..20483
                     /gene="raiA"
                     /locus_tag="PLF20_02610"
     CDS             20184..20483
                     /gene="raiA"
                     /locus_tag="PLF20_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOX79934.1"
                     /translation="MNVNINSVHFKADKKLESFITEKVGKLNQYFDGIIGSEVVLKLE
                     NTDTPENKVTEIRLQIPGNDLYARKQCATFEEATGEAVDALKVQLKKYKAKLKGN"
     gene            20611..20692
                     /locus_tag="PLF20_02615"
     tRNA            20611..20692
                     /locus_tag="PLF20_02615"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20645..20647,aa:Tyr,seq:gta)
BASE COUNT         6078 a         4033 c         4276 g         6319 t
ORIGIN      
        1 tcttcaataa atcgggttgg cggcggctct ttacgatata tggaagtgtg ctgagttggg
       61 aatctattcc tgtttttcgt tcataataat aaatctgttg acaatattcc ttcagctcat
      121 cgctcgatgc tttcccatga tcgaaaacat gaaggatgat atttacgccg actctatgta
      181 aggccctgat tttatagaac acgtcgatta tgcctccata atttggcgga taaggcatat
      241 cgaaagagat aaggtgaagt gttttattcg aggtattttg cataaatagg tattaaaatc
      301 tgtttttcat tttcccaatt aagtttgtct ctggccttta ggcaattatg tctataaaag
      361 tttagtgcag ccttgtcgtc gagcatcgac tgaatgtcgt tagccagaat ttcgggttca
      421 tgaccatgaa tgattcgtcc cacctgataa tgttcgacaa tttttcggat ttctggcagg
      481 ggactggcta agacaggcaa accggcatgt atgtagtcga acagtttatt aggcaggctg
      541 aagtggtagt ttggacaaat atccttatcg atgctcaacc caatatcggc catcatggtg
      601 taattttgga gttcttcggc cggcattctg tcgaagaatt ggatcttgtc attgacgtgt
      661 aaattatttg ccatgatctt tagttgaggc atcacatctc cgccgccaat aataagcaaa
      721 agaacattat cgagaaatct cagcgatgcg atgagttctt cggctcctct gtgaatattg
      781 atgccactgc cttgtaatat aagaatattc ttatcgtcgg gcaaactcaa agatgttctg
      841 tcggtcttaa cttcaagggg ttttaattca ggaacatttc ttgagctaac taccggtatt
      901 ttgtatttat gatggaatat gtcggctaat gactgattca cggtaatcat gatttcgaga
      961 cgaggaagaa tagatcgttc gatggttagc caaattcctc tcaccagggg ccggtgaatg
     1021 acttcgggtg tttcggtgaa aaattcatga ctgtcatata ccaatggtgt ctttctgagg
     1081 agatgaatta aataattggc taagagtgta tcgagatcat tggccaggag taagtcggca
     1141 ggatgaaaaa tcaggtagag aaagagtcgg aggttaaatt cggcatagaa caaggctccc
     1201 cgcctgaaga gtaggtgcat tctgtgcatt ttccatgggt gagagggcag gggaggcgaa
     1261 tgaggcaaaa ttcttcctac gagccaaacc tcataaccca tttcgaaaag caggccgcag
     1321 cttctattga ctcgctgatc ggtggaaaga tcgttgataa ccgaaacaat gacacgtttt
     1381 ttcgccatga gggccgattc gatgtggtgg taaaacgacc tgataaggca tatattgctt
     1441 aagattcaaa agcttagtag gctttggcaa atacgacccg ataaggcgat gttttacccg
     1501 aatagataca ctttcctgct tcggcttgtg gattcaaagg aataaggcgt atagtagcct
     1561 ttgtggcttc cttgattttt tcttccgttt cggttgtacc atcccaatga gccaacacaa
     1621 aacctccttt ttctattctt tcacagaaat cttcccaagt atctactgta aatgtgtttt
     1681 cttctctaaa ttttagagct ctgtgataaa gatttttttg aatatcatcg agcaggtctt
     1741 taatttttag ttcaaggcca tccattggaa tgatagattt ttctaaggta tcgcgtcgag
     1801 caatttctac cgtttggttt tccagatcgc gggggccaat ggcaatacga accggaaaac
     1861 ccttgaattc gtattcattg aatttccagc ccggagtgac gttgtcgctg tcgtcgtatt
     1921 ttacgctgat tccgagtttc agtaatgatt ccctgatttg atgagcttta tggctgatct
     1981 gctctaattg ttcgggtttt ttatagatag gaacgatgat gacctgataa ggtgctaatt
     2041 taggaggcaa taccaggccg ttgtcgtcgg aatggctcat aatgagggct cccatcaatc
     2101 ttgtagaaac tccccatgaa gtagcccaaa cgtattccaa gttattttcg cggttgagaa
     2161 attgaacttc gaaggctttg gcaaagtttt gaccgaggaa atgagaagta ccggcctgca
     2221 aagcttttcc atcctgcatc aaggcttcaa tgcaaaaggt ttcttcggca ccggcaaagc
     2281 gctcgctggg gctttttaca ccttttaaaa cgggtacggc cataaagttt tctgcaaaat
     2341 cggcataaac gtccagcatt ctgcgagctt cggcaaaagc ttcctcacga gtttcatgag
     2401 ccgtatgccc ttcttgccac aaaaactccg ccgttcgtaa aaacaagcga gtgcgcattt
     2461 cccacctcac cacatttgcc cattgattga tcaacaaggg caagtcacgg tatgattgta
     2521 tccaacctct gtaagtgtcc catataatcg tttcgctggt tggtcttaca atgagttctt
     2581 cttctaattt tgcttctgga tccactacta ttccttttcc ttctggatca ttttttaaac
     2641 ggtaatgcgt aaccacggcg cattccttcg caaaaccttc tacatggctg gcttctctgc
     2701 taaaaaacga cttgggaatg aataatggaa aataagcatt tacatgcccg gtgtctttga
     2761 acattttatc cagcgcttgt tgcatttttt cccagattgc ataaccataa ggtttaatga
     2821 ccatgcaacc tctcactgct gaattctcgg ccaaatcggc ttttattacg agctcattgt
     2881 accattgcga ataattttca tcacgtttgg tgaaatcttt tgccattttc ttttgttatg
     2941 gtataatatt tgaaagagtt ttagtgcttc tcttttgcaa tcacgacttg ttagagtgag
     3001 gcgcaaaatt aaaaaataac tttctggcaa tgatatttac ccaaaaggag ggccttatga
     3061 aaaagatttt cagattatta ccattaatga cagttgtttg ggggcttgcg gcttgctcct
     3121 ccgcctatct ggcaaatact cctttcgatg aagcctacga tgtacaaagg agcaatatta
     3181 cgtcccaatc cgctaaaact actccatcca tagcacaaac aaccccctca gctattgaat
     3241 tacaacaaca aaagaattcg ctgaatcctt cggaagagga aagcgatttt tcttcggccg
     3301 agcaacaata ttccactcga attgctgaag gcggtgaaat cgtttctcct cccgaccgta
     3361 ttctctataa tgaatattat gatgaaaacg atgaattcga cttcgacgac tattacgact
     3421 atgcttatag cgttagaata aagcgtttcc atcgccctgt ctatggcgca ggttattatg
     3481 atgattgtta tacgaattta tactggtata cttacgatcc ctgggtttgg ggaatgagtg
     3541 tatatcttgg atatgactgg tggtggtggg attggtatgg aggcccttat ttctctctgg
     3601 gatataactg gggctggggc agtataaact ggcattgggg attcccctat tcgggctggt
     3661 attattatcc acacttcttt taccattatc catggtatcc ctggtattat ggatggggag
     3721 cttaccctta ttacattggt tggtggaatg gtttctggtt tggacattat tacaacagcc
     3781 acgaccccaa ctcctactat tatggtcgca gagtttcccg tcacgaaact aacggtgcgg
     3841 caagcggtgg aaccttcggc gaatattacg agaattacgt aaacaatcaa agaagaggag
     3901 gccaaggcac taatggagtg actcagccat cgggccaggg atcggaatct tcactgactc
     3961 aaagaagaat tcaagctgcg ggagttaatc agcaggctgt aaaggataaa ccaggcgaag
     4021 aaaaaaatct gacgcaagga acgttgaatg aacgtcgtat ttcggcgagt gaatcaactc
     4081 aagtaacacc taaaaccgat caacccgctg taacacctgc acgcattcgt tcgggcgaag
     4141 aaaatcttgc agcacctaaa ccatccaatg aacaaagcga aggaaatgtt tcgaatcgaa
     4201 gaatctcagg aaacgtgaat cagaccgctg aacaaagtca gacccaggat tctcgcttgc
     4261 gtcaaactca ggaaaattat gcaaaacctc aagcttcacc tgccaaccca caacaatacg
     4321 attacaatcg ttatcgtaga acttcgcctg ccacgaatcc agaacaactt gcccgtccta
     4381 agactcaagg aaatcaaaat gacagacctg ttcgcaccta taccccccct acttattcac
     4441 gcccaagaag tagtaacgaa tatacaactc cccgacgcat taccataccc caacaaagtg
     4501 agcaaccttc acaaatgaat cagggagtta aacgtgaaac ccaaaagtct tctgagagga
     4561 ataaaagcta tacccctcct tccagcagtc ggcctaatta ttctgtccct tccagttcta
     4621 attctcacca gaataattcc agcaggccgt cctataacag ctcatcttca caggaacgtc
     4681 actcttcttc ctcattcagt tcgccttccc gatcgagctc caatagtgga ggaggatcat
     4741 cctcctcatc gagaagtggt agtggccgaa gaagataatc aaatcgaaat aacctcataa
     4801 aatcgtataa aagaattttt tttaccattc gcaaattcat tcaaaccata taaaaaagaa
     4861 tagtctatga gaaaaataag tttgatggga atattactgt ttgccacagt gatggttttt
     4921 gcacagaatg ctacggacgc tttgcgttat tcatcggtca attattttgg tactacccgt
     4981 ttcatgacga tgggtggcgc atttggagcg gttggagccg atttttcggt gttgagcacc
     5041 aatcccgcag gcttaggttt atacaaaagc tccgaagtat gttttactcc cttgatttat
     5101 cacgggcaaa ccaaatcgag tttctgggct aataaggccg aagatgacag atataatttt
     5161 gccttcagca atatgggagc cgtgttgaca ttcgaatttc ccagtcaact cgaaacccca
     5221 agctggcgct atgttcagtt tggatttggg gtgaatcgac tgctcgattt tcataaccgt
     5281 atcatggtgg aaggatttaa caatgataat tcccttctga caggttatgt agagtatgcc
     5341 aatcgatatg gtattggaaa aaacagtccg gaagaactcg ctgaaagagc taacctgatt
     5401 ttttatgatg aagctaccaa tcgctattat tgcgatatgc ccgacggagg tgttctgcaa
     5461 cgcaaatcca tcagtaccga aggttcgatg aacgaagtag tgttgtcggc tgcagctaac
     5521 tattccgata aactctatgt aggattgacc tttggttttc cttatttccg ctattatgaa
     5581 tcatcgcttt attcggaaaa cgatgtggaa aacaggaatg aattttttcg ttcctttact
     5641 ttgaggcagc aattggaaac caccagtact ggctttaatc tgaaggctgg tgtcatcgtc
     5701 cggcccgtcg attggctgcg cctcggtgtg gcttatcatt cacccatgtt ctttaccgat
     5761 atgaacgacg tatgggatag ctacattgaa tcttatttcg ataaagaggt tcccaataaa
     5821 tcggtaggat ctcccttggg cgaattcgaa tacgagctca ttacacccat gaaagtgatt
     5881 ggatcggttg catttcaggt gggtaagatc ggattgatta gtgccgatta ccaattcgtc
     5941 gattatcggg atgctcgctt ccatcccacc ggcgaatttg ctgatatcaa cgatattatc
     6001 caatctagct actctaccca aaatataatt agagtgggaa cggaatggcg ttttggaccc
     6061 tttagcgtaa gaggaggcta tggcttttat agtagcccat tcaacaataa catcaacgac
     6121 ggagaaagaa aatcaggttc tttgggagtt ggttaccgat cagataactt cttcgttgac
     6181 cttggctttg tacagaccaa atcttccgaa aaatactttc tctacgatcc tatgaatgga
     6241 gaccccttac cagcagcaaa tactgacttt acatggaata gcctcctgat gaccctcgga
     6301 ttcaagttct aaatcaggtt tttggttcat ctatcagaag ctgtatctgc cataaatgaa
     6361 gatacagctt ttctttttga tcagcgggca gcaatacatt caatctcgac gagcactcct
     6421 aagggcagct taactactcc ataagccgct ctggctggag gatcggttgt aaaatattga
     6481 gcgtaaactt cgttcatctc cccaaaatgt tccatgttgc ttaaaagaca agtacatttt
     6541 accacatcct tataatccaa gctcgcctct tttaaaatgg cgcctatgtt tctcatgacc
     6601 tgatgagttt gttctttaat ccctccttct actaactgtc ccctggaaga atcaataggt
     6661 atttgacctg aaataaatag gaagccattc gcttcgttgg cctgactata aggacctata
     6721 gctttgggcg cttctttaga tgaaattatc cttttcattg tctatataaa atttattggt
     6781 gtatttgtga aaatatgatg atgaatttta ataaagctaa tgaaacgcat aaaaataaaa
     6841 acataatctg attttaggaa aaggcgaatc gaaatttaaa tcttcaatca ttctacatgg
     6901 attttatctt catttttagg caagatgatg ctaacattat caataaatgc agtttcatta
     6961 ggattagttt tccaaaaata aatctttaaa tgcccgtcgg gtaggaattt tttcaggcta
     7021 actttccctt gaattggctt ccagttgtct ttttgtatgg ataaagtatc agggaaaagc
     7081 ggtagataaa gctggtggtt gtctttggta gcatgaataa ccaagcgggt tgtatttatt
     7141 gaattcgaag aagtttcttt cagccatgct tttatttcga ttatggcaag cgaatcctga
     7201 aagggaatgg agctacaaga ggtatttaat atttctgtaa attcatcgga agattccatc
     7261 ttcaaagaaa attcaccttc gaaggattct tcttgggaat gccatttatc aaaggcgtca
     7321 tgaccttcga agccgagccg cataaaaaat atttggccac tattggtatc gaaaaacacg
     7381 ggaaattgtg tttgtgaagt tttttcaaaa agatatacct tgaaatcatg gccgcccaat
     7441 actttgaaag gtgtttcggg ttcgaacgct ttcaggagtt taaaagagga atcggtaaga
     7501 atattttcca agggagtgcc tccttcgtta gggccaaaat gggcatccca agcaagcaat
     7561 gctcctacag ggatttgatc gggtttgatg gaagtgaaat gtatcatcat tttcccttca
     7621 tcatcattca tgggatcttt gttaagaaac aacgcaaata aattgtgata ataatataat
     7681 ttcttgttgg ccaagccatt ttcttttatc cattctgcag cttcggcaat tactttttct
     7741 tcggcatagg gacgaatagg ataaggataa accaatgtgg tgcgcactaa gagaattaat
     7801 actaaaaccg ctgtaatagc aggaggaatt ttaaattttt tgagataatt gattagtaga
     7861 gtatatcctg catggctaat aatggaggca gctggcaaga cggccgcaat aaccctgatt
     7921 aaccccagac tattaccctt gcccgaccac catgaataac tatgagctgc gtaatatatc
     7981 aagaagcttc cagctgttat aatataaacc cctaagtgat tctcacctaa ataaagttct
     8041 cccttcttct ttataatttg aagtccccat cctatcaatc ctaacaagaa taatgacgcc
     8101 atcaggtacc cctgtatttt gtcagtatgg cgaatgaaat gaagtaaact gcctttgcca
     8161 taaatttctg tggcacccga ctggtaagga tttttatgat agatccacag aaaatccttg
     8221 aaaagaaaat atccgatgca gctaaaaata agataaccgg tcaaaagaag aaggatatga
     8281 ggatattttc tatcgactaa aaatgctata gcaactacgg gtagaataac aaaggcttcg
     8341 agccgtataa aaggaagcaa actgaacaaa atggccgaaa gaatatattt tttacttatg
     8401 tatgtataaa tacccgccat taaaaacatg gccatagcga gttcggtgag aggagagaat
     8461 atttgattga agtacaatgg tgtaagaata atgaacggga tagctaacca tgggtgttga
     8521 aatttcattt tatcggccat caggtatccc agccaggctg taattaaggc cattaaaatt
     8581 gaaaagattt tcatgcccga atatccaaat tgtgcaaagg gggtggccag aagagtaaaa
     8641 agcggtcttc cccaaagatc aagaagccac ttgggatcgc tggcagcatg ccttgcaata
     8701 aaataatgag taaatgaatc tccctctccc ccaatccctg gcgataaaaa tgctatgagt
     8761 aggataatga ttgcaaaaat tcccaacaaa atggctgcaa gtattcgatt tttcttctca
     8821 tgcattttag ataggttaca gatttacaaa tttatgaatg tttcattaaa ccctaaaaat
     8881 tcatttcctt tcgaaatatc agacggtatc gattttataa gagcagaagg agtggtcgag
     8941 aatggaattt tcgtcgaatt tctacataaa atctataaat aaacagagga taagaaggaa
     9001 ttttattaat ttgattttct attaccagtc ggttgtgtag ctttggttgt agatgcggta
     9061 gctcatcaat tcgcctgtgg cggaagatct gtagtaaata tagcccgaaa ccacgggaag
     9121 ggtcttccct aaagccgagg tttcatttat attaattggc tgattgatgt tcagtcttag
     9181 attatggcta cctttcccct tgaaattttg ttggatatta tcgatgataa taggagccaa
     9241 gcctttccat tctaaaacaa ctttctcgat ggtaaaggga gaatcgtatt ttgaatggat
     9301 tgtaagatcg agaatggatt tggtaaccat ttcgttttct ttgaggatgg ttttaaaatg
     9361 taaggggtcg ctcacaaaaa tagaggtttt gttagcggct acatcgaaat agaactgttt
     9421 ttgcacatcg gttgttccga agcggtattt tacgaggata ttgttttcgg ggtcttcgta
     9481 acaattctcg taatcgtatt taatgatgac attgtcggca ttattgataa ctgaaccaat
     9541 tcttgcacat tccgatttca cgatgggttt tttagaagtt gagatggggc ggttgcctaa
     9601 acggaggcaa tacttcaatt cgcccaattt gttttccagt tctaccagtt cgatggaatt
     9661 caaaagtgag cgatattgcg atattcccaa attacactga tcctgacgtg catccataaa
     9721 gtaatctttc agactggcca attgagccat gttgccttct atttgttcca cggtttgtac
     9781 cgaatcgatt tgttccagaa gactttcgag ttggaaggtc aattttctgt cgtacaatct
     9841 gaaatcgctc accagttttt tgagttcgaa ttcggagaaa gggtatttga tgtgatagat
     9901 ataagtgatg gttttattag gtcgttgaag cttttcccaa taatattctt cggccttcga
     9961 gagtgaaatg ccctgcaaaa aaggtacctt tcccgattga gtgttggtag tggaggcata
    10021 tttctctaag aataaattca cgttgttatt gtaattggct tcttctttat acatggtgga
    10081 agttgacttg acattttcgg caactgccga tacgatttgt tcttttatgg aggtaagggc
    10141 actttgttgg gcagactgga tgtcagcgcc agttccggaa gcaatgatat aatcttttgc
    10201 aagtccatta acccatttgg gttttttccc gcttttttcc agaaccttgt cttgtgcata
    10261 cgacgaaaag accaataaca tcaatgttgc tcctaagaat agagtttttt tcatagaaga
    10321 ttgaattttc ataatcaaac aaataaaatt tcaaatgatg atgcaaatgg aaaatcagca
    10381 acccggaatg agctgctgat tttcggtgaa tgaaattatt cagggacgtt cgctttcgag
    10441 ttttttcatt tcttcgttaa agacttgttc gaatttcatt ttgtcgtagt caacctgaag
    10501 ttttttatcc ttcgaaactc tattggcaat gttgttcaga acctcatctt tagatacctg
    10561 cactgcggtg aacacttgaa actttccgtc tttcatttgg gtggttttgt agcaagcaac
    10621 ctccacattg ttcaattgct gattaactac ttcgcgggtg agttgttcga atttttgttc
    10681 gaactccgaa cttgcatcga attggcgttc gtttacatat ctttcggaca cggatttaag
    10741 ggtagtattg atgctttggg ctaaattggt gttggcatcc attttggctt tgttgcgtgc
    10801 agtacttaaa tcaatactga tgccattgcc tgtacccctg aagtatttag aatcggtatg
    10861 aaattcttca tcactgcaag gaacgataac ttccttacca aatgtagaat ctactgtttt
    10921 tttggaactc ttgcatcctg ccagcgttac tgcaaaaaga aatgcaccgg ctactaaaat
    10981 cgtacttttc atcttcatag ttgttaagtt taatttgaaa tgttggttga taaattaatt
    11041 gatttattta ataaaggttt attaaggtta ttttttgatg taaatccagc tccaatccca
    11101 gttaaaatag ggtgctgcac cccaataagc ccagctttta gcaacttcct ttacggcctg
    11161 aatcatcatt ttgtcttttt cgatagagtc gtcgtatttt ttcattttga actgccaaac
    11221 attagcacca tcggcaattt gtttctgggt tatctggtcg gctaattgtg aagcttcttt
    11281 gtagcattgc atatcgggtg ttatttttcc gaggtattcg gcggctctgt ccacgtccat
    11341 attagcccag gaggctcttg cacccgagag atactcggtg cacttgtagt cagcatattg
    11401 tttgtagatg tcgatggaaa ggtccataca tttgtcatag cattcacggc aaacatcagg
    11461 aacggagagg agaacaaaga gagcttcctc gtattgcttt tgtcctgcta aagcttgagc
    11521 atttttaagt acaacgtcac actgtgaatt atagaattcg ataatttttt ccttgcctct
    11581 ttctataaaa cctttgaatt gacctgcttt tacattaatt ccacgaatgg cttgtatata
    11641 agctttgtct tcggtttgcc ctactccttt cacctgaata ctggtttggc tgaagatatt
    11701 ctggctaagt gcatccacga tgtagaaagt aatatcgagt gtaagggctt gcataggggg
    11761 tgccgtaggt gtaatttcct tttcgagtac attgattaga gggatcatac aaaatctggg
    11821 gttaaccggc gtagcgccta gtccattttg gacgacaatt tgttccattt tgtttactag
    11881 aagctgatga gccgataggg gtattccttc tgcctgattg ggaacaatag ctgcaatcgc
    11941 caatctgccc aaatcgtcag tcttccccag ataattttgc gcattcaggt tataaatgaa
    12001 cccgaatgct attataaaaa agattattcg ttttttcatc gtatcatgat ttgttatcct
    12061 aaaaagagtt caaaaaccta cctgattaag ccttttttat tttggagcat tttcaagtag
    12121 gtttattttt attttttatt tctcacctaa tatgagccat gcttctccta agcctctgag
    12181 ataaagtttg gaaggaacgc ccaattcgtt gagatattta cgtaaatcgc tcacgaacga
    12241 acgagtatct acagcacgtt cacggcctct ttcatcggtt cgcatcatag gaattctcac
    12301 ttgttcaaat ttcatgaagt tttcactcgc atcggagaga ttaaagcggc cttggacgca
    12361 gttatcgaag aaccaatctt cgatgatttc ggataattct tttgaatcgg ggccaaattc
    12421 tgattcaagg ttttgtcccc agttagccca aactttgacc atcactttca cttcacggcc
    12481 attggcaaac atgtcatcga aatggcgcat caggccagca ttaaaattat ccatgtggct
    12541 taacacggct tcttcgagca gaagttcggg tgatgccgca gttgaaggat ttcctgctcc
    12601 tgctacaccg gttacttgct tggcagtata agcatccaga cctttgaggt tgaaaacgat
    12661 atatttttga ggtccttggc gtttaatatc gaaatcgaga tcgagaatga tatcggcttt
    12721 ggcaacccgt ttcaaggcat cgataggact ttcggccatt tccgaacccg aactcgagct
    12781 tgtaagcata gaagattcgg cacgttcttg ttccagattt ttgagttctt gttccagatc
    12841 ttttagtgga aaccctctgt cggccataat ctggcccatc ttggtaatga ctaagcgtaa
    12901 gtcaggatct gattgcagag cagccttata atctggaagc gtttgggtca tgccttggtt
    12961 ctgaaaaacc attttgtatc cccggctaat gcagtattgg tcgctgggaa ccaccataag
    13021 cacaggtttc ttggcctgtc caaatcctgt gagtacggaa agaataaaaa taaaagtgaa
    13081 tgttaatttc ttcataaaac gatttgattt ttaaaatcca gaatctaatc tgcgggcaat
    13141 gccatcggct tctaattgtt tacgcaaagc gtcgtacatt acctgcacat aaatggcaac
    13201 cttgtatcct tttttgagtt tgatgcggtc ttcggctgca gggtctccgt cgttggttac
    13261 ggcgatgtat tgaaggtatt tacctcctat tttgaagaat tcatcgaaat aatcggcata
    13321 agtaacttcg gcattgggct gtgaacatat tggaggtgtg ggtgccgaac cggctccacc
    13381 cggcagacct ttgaagatac aggcagcaac tgcagcttct ttggcattca ttactgctgc
    13441 attcacatct tttccataac cccatacctt gaaaactttt gtgccatctt ggccaacgcc
    13501 aacgggctca atttcataat tgtactggct gctgtaaagt atcttacgct ccttgcgcga
    13561 ttgagcactc gcagtaacaa tcattaaact gaccaatact agtgataggg taagtgattt
    13621 tgttttcata agcataatcc gtttatattt aggttagaac atgattttaa gtcccactaa
    13681 gccagaaaga cctttcctat cctgaaatat ttcatcccat tttattccaa ggtcgctttt
    13741 atctttattg cttgcattac cgatgagtgc gtaataattt atgccgccgg ttaattgtat
    13801 gttatgccgt aagttgatgg caagattacc gccaaatttt agaaacaacg tggagagatt
    13861 acccttgtcg gccttatcga tttcatcgct cgaagctgat tctaatccaa tgccaatgta
    13921 aggtcgtaat tccatattgc gcataagcat caatccctgt gccaaaccta caccataatg
    13981 gataaaggta acatcctcag tggtggcctt tgtatagctt ggataatgat attcgtattc
    14041 cttttgttgg ccgccacctt ctaaataaat aaagaaggct ttaagaccaa tgaagcgacc
    14101 cagtctaaaa tcgagccttc cataaggtcc accaatttct cccatttcgt atccagctaa
    14161 aatttctatt ccaatatcat tttgttggcg aaccaaataa cccgtctgaa gttttcggcc
    14221 tgcagtctga taaaaggtag aagtaggcat ttcgccagta gcaagctgac gattgtcggc
    14281 aattttcgaa gtagctctga ttactccacg gtaaacgggt ttaacggtat tagttttttc
    14341 atcataaagg tattcataaa caaaatagcg gtgatcgcat ttgagccctt ctttcttccc
    14401 gattttgacc tgaatgggtt ttaccttata aatggtggct ttaaccataa aatcttcata
    14461 ttttctttcg aggtaatata aggtttcgtc atagccactc tgaacgagtt ccattaaaag
    14521 ttcatcatcc gatttttgtt tggtgagttt gcccagttgg gtatcagggt tagcttgcga
    14581 cgcactgagc gaatgagtag ttttggtgac gaattcgatg gggatttcga gctgttcgaa
    14641 tttttgaagt ttttcggctt tcacttctgg actatcttct ggatagatcc agcaatcgta
    14701 tagagcggct tgaatttctt cattgaaatc gatcttgtat aaataagccg taacagtggc
    14761 ctcccagcca tgttgatcgg ctactttggc ttcgctcatg ttttttacat tggcaaagtc
    14821 gaatactaag atataactgc gctgaataag tctgttgcca taatcttgca ataaggcatt
    14881 accacgtttt gtggtttgtg ccttgatgta ttcggcatcg ctcgcattaa acatgcctct
    14941 ttcaaagata aggtccatcg tcatggtacc atcttctttt ctattatacc atttgctgat
    15001 aaccgacttg gctatctttt gatttgtcag ataatccttg attgctactt cgggaacaat
    15061 ttcggtgttg gttctcgaat aaggcgcctc aaagattaca ttcgcaaggt tgttgttgta
    15121 atatttatca gaaaacacga cctgttggaa ttttgacgcg atttctgtta catggttctc
    15181 gcctggaaat ttcatgtaca ggcaggtaag agagcttctg tcgtaagtcg aagggattac
    15241 tttggcctga tcgatactct gggcttgaac gccaattaat ggcaccaatg atgtggccag
    15301 cacgaataat agctttgaat tcatagttca ttaaggtttt ggtggattga tgttgaaatt
    15361 tttggtaaaa gaacaaaatt tttcgaatga aagataaaat ctggaaaaac ttctgaagaa
    15421 tcatatcctg taatctatca agagaaagcg attctatttt tgctaataaa ctgaatttca
    15481 ttccattgtt tttttcgtaa agaagtcagc tttattaaat ttcaaaatat cagtagaaaa
    15541 tttttcatcc tctccttaaa aatctatact ctccgactct gtttcgaaat acgaaaagtt
    15601 gaattttcaa aagcaaggta aaaatagaaa ttatatttcg aagtttggtt atccactttc
    15661 cgggctatag aataattttt ttgagaaatc tattttgagt cctttcactg ctcccttgta
    15721 ttgatgataa tggtctcgac ctaacattaa aaagggagcg agagagccat tgctttatac
    15781 tttattctgg ctttgaagtt cgtaagaaat ggtttatata gaaaagaatt ggtgtggaaa
    15841 tatgagggat gcctggataa ttcgttcgat tcattgtatt tcgatcgata cattatcggc
    15901 tttatagaca atttgataaa aatatattga aaaccggcac ttcagaaaat tatccttaga
    15961 tcaagaagag atgagtttat ttcgaagagt tccattttac ggtattaagg tttctggttt
    16021 tattgccagg ttctgaaaat atccatagag agatgaaggt gagcaatcca ttgagcaaca
    16081 gtaattcata gccaaattta taaccaccga gccatttcac cgagttagaa ctgatcagat
    16141 agcatagagt agggattaga atagcaatat aagggcttac gttatctttt aattttcggc
    16201 ttgtataaag gccaaacata aaaattccga gcagtgggcc ataagtataa cctgcgatcg
    16261 tatagatagc attgattacc gttttatcgt tcaaagcttt aaaaaccata atgattgcga
    16321 gcatcagcat agatacacca agatgaaccc accggcgcgt ggtatggctt tcttctcctt
    16381 ttattttcag aaaatcaaca caaaaggagg tcgttaaggc agtaatagcc gaatcggctg
    16441 atgagagtgc tgctgcaaca atacccatta aaaaactgat tccaatgatt ttggggaaaa
    16501 atttcaaagc aagtaaggga taaagctggt cggtattgtg ataaatacca ctggccttat
    16561 cgaaatagaa accggatgaa tcgtgaaaat tcaagccttt gctattgata tagagaaata
    16621 gaagagcacc aagggataag aaaatcaaat ttacggggat caatatgaaa ctgtaagtta
    16681 tcatgttttt ctgagcatcc ttgatgttgg ggcaagtgag gtttttttgc atcatgttct
    16741 ggtcgagacc agtcatcaca atacaaataa aggctccaga aaggaattgc ttgaagaaat
    16801 tttggctcga acgccaatcc cagttgaata tttttgaata atcgcttttg gctatagttt
    16861 tggtcaagcc tggcaaatcg agattcattt ctttcccgat ggcaataacc gaaaagaaaa
    16921 ccgctgccag cattagggtg gtttggagtg tatctgtcca gacgatggta cgaatacccg
    16981 aacgataggt atatgcccag ataagtgcaa gaataatgat aacagttaca aaaaaaggaa
    17041 tgccgaaagc atcgaaaaaa gccatttgca ggactgaagc tgccaaatac aggcgaagcg
    17101 aagcccctaa cgattgagat ataaagaata aggttgcccc tgtatgatag gacacatttc
    17161 caaaacgttg ttgaagatag gaatatatag agatgagtcc caaacgataa tatattggca
    17221 tcaatataag agcaatgaat aaataaccca acggataacc aagaacaaac tgaaagtaag
    17281 aaaatccatt attacccact tctccgggaa ctgaaattaa ggaaacaccg gatatggaag
    17341 caccaatcat tccataggct actaaaatcc atggtgattt atggttccct gtgaaaaatg
    17401 tagcatttga tgtgtttttc gaagttaaag ccgaaacaag catcagtatg gcaaaataca
    17461 ctgcaagaat tccaaaaacc aaggtagggc tcataactaa ttaaggatat agcaaatagg
    17521 gtttacgttt gagcatgaat tcctgtaatt ggggctgcca gctttcacgt atctgatttt
    17581 catcgagacc atcgagaatc tgcgacctca ataacgaaga tcctgcaagc ttgtcgaaga
    17641 aatcgggacg tttgaaaaat ttttctcccc aattcgataa atgataaaaa tctaatatga
    17701 atttcagtgt gaatcgagta tgtaaagaat cccgccgaag atctattcca tagcaagtat
    17761 cgcctttatg gagaggatga atagattttc ctggaattgc tttaggtaca aaagtaaagt
    17821 taccaaaaat gggagcaggg aaacctataa cctgaaatgg aaaatcagtt cccctcccta
    17881 ccgagatatc agtggcttcg aacaggcaaa ggctgggata gagacgtatg ctttgcgtat
    17941 tgggaaggtt cggactgggt tcccgatcga gaattacggg tgtggaatgc gtatatccat
    18001 ctaaagggat tatagtgagt tcacatttca ttccgtttct taaccatcct tctccgttga
    18061 tcattcttgc cagttcgcca atcgtcatcc cataaactat gggaatagga ttcattccta
    18121 caaatgaacg gaaagataaa tcgagcacag ggccatctat ataatctcca ttgggattgg
    18181 gcctgtcgag aaccaacaat ggaataccct ctttggcaca gctttccatc atataatgaa
    18241 gagttgaaat ataagtaaaa aatctaaccc ctatatcttg aatatcgaac actatgacat
    18301 cgagaccact catttccttg ctcgaaggtt ttttctggtt accatacaaa gaaataattg
    18361 gtagtcccgt tttttcatca atagtatttt gaatgaattc cccagcctcg gcctctcctc
    18421 tgaatccatg ttcgggagca aaaatttttt ttacattaat tcctgctgaa atcatgctat
    18481 cgataagatg cttttcgcct atcatggaag aagcatttgc aactactcct atggctcgat
    18541 caccgcctaa cgaagaaaaa tatgaatctg ttttccatgc gccgggaatg attatatttt
    18601 gaccattaac cgatctgctt aacaaaatga taggcaacaa aaagagaaag aaaactattt
    18661 tagaaggaat aggcttcatt atgtggcaaa attataaaga acgatacata atgaacaacg
    18721 aatcacggat cgcattcact cctaatgggg ctcgaataat gcgggtttgt cttcgaaaat
    18781 gttgacatgc cgcccaaaat gatgctcgaa cgaaattttt tcatcttcat ataactcagt
    18841 ccccgctatc tctcatcctc cattttgccc attcacaaga agccacgaat aactatagat
    18901 atcatcaatc aacattctca cattgctcaa ccaataaaaa ttgtataact ttgcagccct
    18961 aatttttatt cactaattaa aagcgaggta ttttttatga ttattgttcc tgttaaagaa
    19021 ggcgacagca tcgaccgagc gttgaagaaa ttcaagcgga agttcgaaaa aaccggtgtg
    19081 atgcgtgaat tacgcgaacg ccaaaaatat acgaaacctt ccattagacg acgcgaagag
    19141 aaaactcgag ccatctttat tcaaaaactc agggatcaag agttgattta agattcttga
    19201 aattacagga agaaaactat ccggacagaa attttttcta tccggatttg ttgtaatttt
    19261 atcccaagct aaattttgga tgatagaaag ttttatcaca tatctggaaa gagaaaaacg
    19321 gtattcaaaa tacacatgta cctcttattc gattgattta caacaattcg aatcttattg
    19381 tcaattgcaa tatggccact cggatatcga tactgtaagt tctgatgtga tcagaagtta
    19441 tgtggtttgg ttgatagaaa aggattattc tcatcgaagc attcaccgga agattatttc
    19501 gttgcgtcag tattacaggt atttgattag attgggatat attcatgcta atccggctcg
    19561 agatgttcct gtaccaaaga ttccagaacg aattcccatg tttgtaagtg aagaaaacat
    19621 gaaaaatgtg ggtaatgttg cgtctaagag gaagaatgat tatgaaacca tacgggataa
    19681 attaatcata gaaatgctat atgccacagg aatgcgtgtt tcggaattga ttcatctaag
    19741 gatttgcgat ataaatcttg aggggatgaa catcaaagtg atgggaaagc gaagcaaaga
    19801 aagattgatt cctattggag aaaaattagc agatcattta cgacaatatc tcgaggtgct
    19861 tcgacgtttt ggttttgtag cttctcctga atcgtggtta ttttcgaccc gaaaaggcca
    19921 gaagatttat cctttgcttg tttatcgagt agtaaatgaa ttcttgagta aagtaactac
    19981 attggaaaag aggagtccac atattctgag gcatagtttt gccacacagt tattgaatgc
    20041 cggagccgac ctgaatgcca tcaaggaatt gctgggtcat tctagtttgg cgtccaccca
    20101 aatttataca cattctgact ttgagacgtt aaaaaaaata tatcaacaat cacaccctaa
    20161 agcttaaaag aaaggagtaa atcatgaatg taaacatcaa ctcagtacat ttcaaggccg
    20221 acaagaagct ggaatcattt atcacggaga aggttggaaa attgaatcaa tacttcgatg
    20281 ggatcattgg aagtgaggtc gtattgaagt tggaaaatac cgacacacct gagaataaag
    20341 ttacggaaat caggttgcag atacctggga atgatctata tgcacggaag caatgtgcca
    20401 catttgagga agcaacagga gaagccgttg atgctttgaa agtccagttg aaaaaatata
    20461 aggccaaact caaggggaac taaaaatttt gttcaaaaag tttggttcgt atcgtaataa
    20521 gatttacttt tgcagtccct tttttgggac tgttttttta tagcccgtga tggggggttt
    20581 aaaaattttt taatgataga ataaagctga ggggagattc cagagcggtc aaatggggca
    20641 gactgtaaat ctgttggcag tgccttcgga ggttcgaatc ctcctctccc caccgaatga
    20701 agaaac
//
LOCUS       DAOORU010000020        20522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_3175_length_20522_cov_5.355401, whole genome shotgun sequence.
ACCESSION   DAOORU010000020 DAOORU010000000
VERSION     DAOORU010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20522
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3175_length_20522_cov_5.355401"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PLF20_02620"
     CDS             <1..786
                     /locus_tag="PLF20_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOX79935.1"
                     /translation="YFYAVNSSAITETLFESEFFGHTKGSFTGANENKTGWFEVANKG
                     TLFLDEIGDLPLSLQTKFLRVLEERKISRVGGTQEIGIDVRIIAATNQDLEKLTEEKQ
                     FRLDLYHRLSAFIIHIPPLRERKEDIPLLINYFIDDFSKKMNKKITRIDKEVVDTLID
                     YSFPGNVRELRNMVERAIILSEGDRLVADDFQFDKISKKISRTNNNLEVLDLEILERN
                     AIIKAIHQSNFNKSAAAKLLNISFQALDRRIKKYHLVFEKRLG"
     gene            855..1787
                     /locus_tag="PLF20_02625"
     CDS             855..1787
                     /locus_tag="PLF20_02625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79936.1"
                     /translation="MSEFDFTEYTKCKLCPRNCGVDRSKEASGFCHANDRLAIGSICR
                     HTGEEPPISGQYGIANIFFMHCTLQCQFCQNMQISQNKMVLDPYLYRVHEVTARVMKL
                     LEEGCKAVGFVSPTHYIPHVKAIISELKSNGYHPPFVYNTSGYENTEQLKELEEWVDV
                     WLPDFKYADRSLARRLSMASNYPEVALKAIREMFRQKGSNVIVNKEGQAESGLIIRHL
                     VLPGYIENSIKVLETIADEISTNVYISLMSQYNPQFYQKMDVALQRPIDLQEYEMVVQ
                     RFYELGFHNGWIQELESHKTYNPDFEQEHPFEMS"
     gene            complement(1957..3108)
                     /locus_tag="PLF20_02630"
     CDS             complement(1957..3108)
                     /locus_tag="PLF20_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79937.1"
                     /translation="MNSFVSFTDAPPLRLLRFAADAIDGIEKGLIVFYQMPGNKLIAQ
                     SVERNHDEGWEVRSVLVEDFYEFFRSERQRKGAYQWLGKDDLPFEVHNQAFSHTIFDE
                     FDRTILLLRFLDETNQGYDLLYLYFNPRFKSAFLSSEKQSFSTSEKALTAQILHRLLA
                     TFLVLMREERESFGQLMNNTRQLANRFQNERNKTELLEKQAGQSLVEYSKDILNELAC
                     KYNRKALLSPEAETLIRNFSGKLSHLKELLTNAFHFSLILNEPNQELMIQDWHMDIEK
                     SPLKSDKRRKEKYEHLGRTGDLLNRLEFAARSVLNDRLPLTGKNVGSHCDKPISAPAI
                     SDALKKHRSEIIALFEQYPDQWPIIRKDFAPIRNILIASGEKGKNRRVG"
     gene            3315..3584
                     /locus_tag="PLF20_02635"
     CDS             3315..3584
                     /locus_tag="PLF20_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79938.1"
                     /translation="MRSEFFYEIQHAREKYLDELSKLKDKELDGHENDKSLQNKSIEP
                     LSSNHNYCIHEDFEQRGCIPSLNIFAKLIKAIYIAFLSAKYNHNE"
     gene            3821..5359
                     /locus_tag="PLF20_02640"
     CDS             3821..5359
                     /locus_tag="PLF20_02640"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOX79939.1"
                     /translation="MDATLVEEIKRYISTNDSASLIKAMHNYHLGIDDFLDTEKSETA
                     LSIAIKLKQKDMVAFLLQSGANPNLCVRSETLLILAILSDEPEIVKLLLDHDALSDVS
                     DDNGNTPVALSIKRNQLLILTYLVKAGANLHAKDKFGKRPIDYIHSSTDQNLAIYLQH
                     ATLMQEKALHWPEYTDGPFVEWKDSSSFELKYFYTRDRRLCEKSELFHSVKLPFVLKS
                     SILFHPVEIKRHYDRSPGICDMPERLLAIGDLHGNLQSLLSLMIGNKIIDAAFNWIWG
                     NGQIVFIGDLFDRGCQVTELIWFVQHFESQAQINGGRVHLILGNHEIMALIGDHRYLN
                     EKYRNICEYFSMNYWEFYTPDSELGRFIRSKPAILKIGDILFVHAGIGPEHLSKMLSI
                     NEINDVIYNTLSDNKRKLNELEIFMLLDDGPLWFRDLLHEYSYFQTLSESYIDSVLSF
                     YEVSHIIIGHSEIPEINSRYNGKVIAINVPFYHSMYTPQALLIECNQYFIIGADGEKK
                     LFLK"
     gene            complement(5537..6655)
                     /locus_tag="PLF20_02645"
     CDS             complement(5537..6655)
                     /locus_tag="PLF20_02645"
                     /inference="COORDINATES: protein motif:HMM:NF025115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4163 domain-containing protein"
                     /protein_id="HOX79940.1"
                     /translation="MKKLFLAIMAFSVNLITGFHLEAQNYQHFETPDNSSPKCYVDLI
                     ILENDNAQLVLDMPSSAELDFNSPTIFYGKIDKNTGEASLQSMNEKLWLNFLYTQKQL
                     KNPKLNQGQKQLYLPSFNLTWDRGNLPLKVIHQNKKQPLFSMKETPKAVVDIIFPLPQ
                     YSINPRINDSIMFHLFMSYFGQEYPKGKTILQAIDNQIDKFCKSYITENQEYYDRSLP
                     DAAFNWERTYSFQVMCNSRDIFCIRILNYAFTGGAHGMEVSKYQTFNLKNGSVIHLAE
                     QLDLEKQDLIIDLITKNLAKTYAEETRGNLLEAGFFSDHIELSNEIYITTNGLGFVYQ
                     PYEIAPYSFGSIEVFLEKEKILPFLKQNSFLINWFNKH"
     gene            complement(6769..8166)
                     /locus_tag="PLF20_02650"
     CDS             complement(6769..8166)
                     /locus_tag="PLF20_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX79941.1"
                     /translation="MEKVRQTLRDSAVARWTALALVAFTMLTGYYLTDVMAPLKGLLE
                     QQLSWTSTEYGIFTSAYGWFNVFLLMLIIGGIILDKMGVRFTGLASTIVMITGTAIKY
                     WAVSTHSLDGQVWNFLGLFQIKAQVVMAALGYATFGVGVETAGITVSKIIVKWFKGKE
                     LALAMGLEMATARIGTALALSTSVPIAQKLGHVSKPILICLIMLCIGFISFFIFTFMD
                     KKLDASQEAYDKDNEVVANEESFKVSDILKIISNRGWWFIAILCVLFYSAVFPFLKYA
                     ADMMVNKFGIDQSIAGTIPAVLPFGTILLTPLFGNLYDRKGKGATIMIIGAILLIFVH
                     AIFSLPFINQWIIAVLLIIVLGIGFSLVPSAMWPSVPKIIPEHQLGTAYALIFWVQNW
                     GLMGVPALIGWVLDKYCVTGQKLVEGTLVNTYNYTLPMIIFTTLGVLALIFGFLLKAE
                     DKRKGYGLQLPNIQK"
     gene            complement(8462..8535)
                     /locus_tag="PLF20_02655"
     tRNA            complement(8462..8535)
                     /locus_tag="PLF20_02655"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8499..8501),aa:Arg,seq:acg)
     gene            complement(8600..9514)
                     /locus_tag="PLF20_02660"
     CDS             complement(8600..9514)
                     /locus_tag="PLF20_02660"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: protein motif:HMM:NF000801.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HOX79942.1"
                     /translation="MGFSITFLGTGAATPTLRRGLSATVANVDEKLCLFDCGEATQIQ
                     MKRFKVKALHIQHIFISHLHGDHFFGLVGLLFTYHIGKRKNELHVYGPPLLEEILRIQ
                     MGISVKELNYPLVFHPLSSQNLEMVCEEDNFTVSAFPLQHRLPAYGFLLKEKERPRKV
                     LHQFIEDYHPHYTDIVRIKSGEDYVLPDGRILSNEEITFPDPPISFAYCFDTAFYAPI
                     AEVLKNVDLLYHEATFMHDKAELAAEKGHTTTIQAATIAKKALAKRLMLGHISSRYRN
                     LDSFLNEARNIFSETYLAEDGMTITLRK"
     gene            complement(9953..11752)
                     /gene="rpsA"
                     /locus_tag="PLF20_02665"
                     /pseudo
     CDS             complement(9953..11752)
                     /gene="rpsA"
                     /locus_tag="PLF20_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
     gene            12190..13641
                     /locus_tag="PLF20_02670"
     CDS             12190..13641
                     /locus_tag="PLF20_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79943.1"
                     /translation="MKFSRYSLFFPSVLVIFLVFSFFSCTKDEPSTDSSYQLTFSNDS
                     VVFDTVFTTIGSVTQELRIYNKNNKKIKISYLRLAGGEQSAYRLNIDGQNTWIKNDVE
                     LDKDDSLFIFVRVRINPNDKNSPFLVSDSIEFLTNGNYQNIKLIAYGQNAYYHTPDHF
                     PADLPPYSIIECSQTWTSDKPHLIYGWAIVDSASTLVMEGGTRVYFHHGAGLLVKKEA
                     SLKVNGEAENKVIFRNDRLDQFYKDLPGQWEGIYFADGSPESYIHYAEILNGTTGLMI
                     EQRNNNRRARFEITETLIRNMKKGGLRSTGCNLYSVNCVIVNCGSYALQLEGGIHEFI
                     HLSIGNFWTSGIRMSPSLLLTNYYMDGENQINIEPTSFNFTNSIIFGNQYEEISIDQG
                     EQGTGFPYIFDHCLLKTSLNTTDESIFPSCLINIDPLFVDIEKPKLEPDSLSPVLHAG
                     KSAGVLQDILGRQRDMQRPALGAYEPDYSGGKR"
     gene            complement(13668..15446)
                     /gene="argS"
                     /locus_tag="PLF20_02675"
     CDS             complement(13668..15446)
                     /gene="argS"
                     /locus_tag="PLF20_02675"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOX79944.1"
                     /translation="MFAQLLAQQTGYAIEALYHHIVQPNQVPVNKTKKEFTGDFTIIT
                     FPFAKISKKSPELTAREIGEWLKNALPEIESFNVIKGYLNIILKDDFWMEFLDKNYRN
                     HSFGLNPQKQGQAPVLIEFSSPNTNKPLHLGHIRNNLLGHSVAEILEATGLPVVRVNL
                     VNDRGIHVCKSMLAWKKWSNGETPESSGLKGDHLVGNYYVLFEKKYKEEVNQLKANGL
                     DEETALRTAPLMLEAQEMLRKWEAGDAETHSLWEMMNSWVYEGFNETYKRLGISFDKI
                     YYESETYSLGKKIVEEGFKAGVFYQKEDGSVWVDLTSDNLDHKLLLRSDGTSVYITQD
                     LGCAELRYQEFSPQKMIYVVGNEQNYHFEVLKLILQRLGRTYADKIFHLSYGMVDLPE
                     GKMKSREGTVVDADDLIEEIILTAREITLELGKATDLDEYEAHELFSMIGLAALKYFI
                     LKVDPRKNILFNPTESIDFDGNTGPFIQYTHARIRSVLRKANPWSFTPQKNMTFYEEE
                     KEILKILYDFPSVIKQSAKELDPSQIANFAYELARSYNQFYHNLSILHEPDEQRMLTR
                     LAISQFTAQVIRISMHLLGIDVPERM"
     gene            complement(15486..17174)
                     /locus_tag="PLF20_02680"
     CDS             complement(15486..17174)
                     /locus_tag="PLF20_02680"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HOX79945.1"
                     /translation="MVINILSIIGALCLFLFGMKLMSEALQKMAGQNLRNFISGLAQN
                     PVKGVLSGLVITAALQSSSATTVMIIGLCTANLLTFPQAFSLIMGANIGTTLKAWIIA
                     FIGYNFDLSQFALPIIALCFILLFSKKATIRALGEFFMGFALLFMGMNFLMMQSQSLT
                     THPEWIGWIQSHNHRGFSSILFFVTMGFVLTAVLQSSSAMLVFTFALCASGIVGYENA
                     AAMVIGENVGTTITANAAAIVGNYKARRAALSHFLFNVSLMILALAFFPFIIQMMTSF
                     TKGLTGQNPSSDSQSIPFALSAFHTFLNLITTAIWINFIPQMSAIVNTIIPVKYSTPY
                     QIRYFNPFFISTSEFNLLHLKHEIAVLSSEVIELFRFVISSVVPGDTSNLIQQNEDAE
                     RLYQKIQFHHTEILNYIQTISFDEMSADGQVRYRTMLLTLENFQVIVKVNQKLLQTIQ
                     EKSKEKIWFSPKQHRDLMGLLDEVDSAFQLLVNNLNGEYHKVNIAESQLIEEKINKMR
                     DKIKEKLPRKIEQGKINPLSVDYYKSLVDAAENLGDLMMEINRKLAECGKKTMP"
     gene            complement(17268..17948)
                     /gene="hxpB"
                     /locus_tag="PLF20_02685"
     CDS             complement(17268..17948)
                     /gene="hxpB"
                     /locus_tag="PLF20_02685"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HOX79946.1"
                     /translation="MNSFQAAIFDLDGLLIDSEPLWEEAEINVFARLGVKITSQMCQK
                     VKGFRVDETIRFFCQCTSFDALDPDLIEQQIISEVKRSIYEKARGLPGCVYILHFFQS
                     RDIPLALASASPYDIIDLALRKLSIHQHFKIIYSATEEKYGKPHPGIFFTTAEQLQVE
                     PQNCIVFEDALNGIIAAKAARMKAVAVPADQTSMDLRFVLADLILPSLLQFKEDHLEY
                     LINPHHGG"
     gene            complement(17956..19113)
                     /locus_tag="PLF20_02690"
     CDS             complement(17956..19113)
                     /locus_tag="PLF20_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOX79947.1"
                     /translation="MANFDSIIPREGTSCVKYDLRNQVFGTEEVVPMWVADMDFATPE
                     FILQALHRRLNHPLLGYSFRAQEFNESLANWLFRRHALSIDPQIVSFSPGVVTGLMVL
                     TLAFTNPGDKIIVQPPVYPPFFEVPRAHERIVINNPLKFDGERYSMDFDDLREKAPGV
                     RMLILSNPHNPVGRAWERRELEQLSEICIQNDIMIVSDEIHSDIIFPGYRHTPLLSLD
                     KEIGMRSASCFAPSKTFNIAGLSTSAILIPDPQKRKIYNDTLERFHLQMGNLLGNIAF
                     EAAYNQGEDWLEELLDYLHQSVRIATHYFEEHLSPIHLVQPEATYLLWLDFRGLHIPD
                     DELHAHLIHKAHLGLNRGEEFGIEGKGFMRMNIACPHAILEKALESLKNIL"
     gene            complement(19177..20286)
                     /gene="gcvT"
                     /locus_tag="PLF20_02695"
     CDS             complement(19177..20286)
                     /gene="gcvT"
                     /locus_tag="PLF20_02695"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOX79948.1"
                     /translation="MKNTPFTKFHIALGAKMVPFAGYNMPIQYEGVISEHLTVRKSVG
                     VFDVSHMGEIWVKGPKAFDLLQYITSNDVSKLYDGKIQYSCMPNGRGGIVDDILVYRI
                     TAESYLLVVNAANMEKDWNWITQHASDRGMKIGTDVYNASEETGQLAIQGPLAMKSMQ
                     KLTSESILDMEYYSWKNLEFAGVKNILFSTTGYTGSGGCEIYMPNEAADTIWNAVFEA
                     GKEFGIKPIGLGARDTLRLEMGFCLYGNDIDDTTSPLEAGLGWITKFTDTKNFIDKEY
                     LLQQKKEGVKRKLVGFVLKEKGIPRQHYSICNAAGNKIGEVTSGTMSPSLNIPIGMGY
                     VPAELSQPGSEIFIEIRGKLIKAEVVKLPFYKGEQ"
BASE COUNT         6185 a         3935 c         4052 g         6350 t
ORIGIN      
        1 tacttctatg cagtaaactc ttcggctatc accgaaacac tgtttgaatc ggaattcttt
       61 ggacacacca agggttcgtt tactggcgca aatgaaaata aaaccggatg gttcgaagta
      121 gccaacaaag gcactttgtt tttagacgaa attggcgatt tgcctctgag cctgcagact
      181 aaatttctgc gcgtactcga agaacgtaaa atcagcagag taggagggac tcaagaaatt
      241 ggtatcgacg tcagaatcat tgcagcaacc aatcaagacc tcgaaaaact taccgaagaa
      301 aaacaattcc gcctcgattt atatcaccgc ctcagcgcat tcattatcca tatcccacct
      361 ctgcgagaac gcaaagaaga tatcccattg ctaatcaatt attttattga tgatttttca
      421 aaaaaaatga ataaaaaaat cacccgcatc gacaaggaag tcgtggatac tcttattgac
      481 tattcattcc ctggcaatgt gagagaattg cgcaacatgg tcgaaagagc gatcattcta
      541 tcggaaggag accggctggt tgccgatgac tttcaattcg acaagatatc gaaaaaaatt
      601 tctcgcacaa ataataatct cgaggttctc gatctggaga tcctcgaacg caacgccatc
      661 attaaagcaa tccaccagtc aaatttcaac aaatcagccg ccgcaaaact tttgaacatt
      721 tcttttcagg cattagaccg aagaataaaa aaatatcatc tggtctttga aaaacggctg
      781 ggttgaattt catcatcgta tcgaacatat tttcgccatc acagcatgcc gttttataga
      841 acatcattag aaaaatgagt gaattcgatt ttactgaata tacaaagtgc aaattatgcc
      901 caagaaactg cggagtagat cgttcgaaag aggcatcggg attttgccat gccaacgata
      961 gacttgcaat aggctctatt tgcaggcaca ccggagaaga accccccatc agtggtcaat
     1021 atggtatagc aaatatcttt ttcatgcact gtactttgca atgccaattc tgtcagaata
     1081 tgcaaattag ccaaaataag atggttctgg atccttatct ctatcgtgtt catgaagtta
     1141 cggcaagagt aatgaaacta ctggaagagg gttgtaaagc cgttggtttt gtgagcccaa
     1201 ctcattatat cccccatgta aaggcaataa tttctgagct taaatctaat ggttatcatc
     1261 cccctttcgt ttataatacc agcggttatg aaaacacaga gcagttgaaa gaactggaag
     1321 aatgggttga tgtttggcta cctgacttca aatatgctga tcgatcatta gccagaagac
     1381 tttccatggc gagtaattat cccgaagttg ccttgaaggc catcagggaa atgtttcggc
     1441 agaaaggttc taatgtgatt gtgaataaag aaggtcaggc cgagtcggga ttgatcattc
     1501 ggcatttggt attaccgggt tatatcgaaa atagtataaa agtgctcgaa accatagccg
     1561 acgaaatttc tacgaatgtt tatataagcc tgatgtcaca atacaatcct caattttacc
     1621 agaaaatgga tgtggcttta caacgtccaa tcgatttgca agaatacgaa atggtagtac
     1681 agcgatttta tgagcttgga tttcataatg gctggataca ggaattggag agtcataaaa
     1741 cctacaatcc agattttgaa caagaacatc cttttgaaat gagctgagta gagaaaatgg
     1801 agaatgtgag taccaatgcc agaatagtat tgacgagagg tcaattcaag aaaatcgtca
     1861 atttttgctt gagccctatg agttggtaat acatgataag tattcgatct gctccgtttt
     1921 atggcctgac acttttacgt tgacacagga tatggttcac ccgactcttc ggtttttccc
     1981 tttctcgccc gacgctatga gaatgtttct tataggagca aaatctttgc gtatgatggg
     2041 ccattgatca ggatattgct cgaataaggc gatgatttct gaacggtgtt tttttaatgc
     2101 atcgcttata gccggagccg atatgggttt atcacaatgg ctgccaacat ttttccccgt
     2161 tagaggcaat cgatcattca acacgcttcg agcagcaaat tccaaacgat tcagcaaatc
     2221 acccgtgcgt cccaaatgtt catatttttc ctttcttcgc ttatcggatt ttaatggcga
     2281 cttttctata tccatgtgcc aatcctgaat cattagctcc tgattaggct catttaaaat
     2341 cagtgaaaaa tgaaatgcgt tggttaataa ttctttgagg tgagaaagct ttccggaaaa
     2401 attcctgatt aatgtctcgg cttcaggaga aagtaaggct ttcctattat acttgcaggc
     2461 taattcattc aatatatcct tcgaatattc cacgaggctc tgcccagcct gcttttcaag
     2521 cagttctgtc ttatttcgct cattctgaaa tctattggcc aactgacgag tattattcat
     2581 caattgcccg aatgattccc tttcttctct catcaatact aaaaatgttg ctaacaaacg
     2641 atggaggatt tgtgcagtaa gtgctttctc gctagtgcta aaagattgtt tttcagaaga
     2701 taaaaaggcg gatttgaatc ttggattaaa atacaaatag agcaggtcat atccctgatt
     2761 ggtttcatct aaaaatctga gcaaaagaat ggtgcggtcg aactcatcga atatggtatg
     2821 agaaaaagcc tgattgtgta cctcgaaagg taaatcatcc ttccctaacc attggtaggc
     2881 ccctttacgc tgacgttcac tccggaaaaa ttcatagaaa tcttccacta atacactcct
     2941 cacttcccaa ccttcatcat ggtttctttc gacactttgg gcaataagtt tgttgcctgg
     3001 catttgataa aatactataa ggcccttttc gattccatcg atagcatcag cagcaaaccg
     3061 gagtaaacga agaggaggag cgtcagtaaa cgaaacaaat gaattcatga aaaaaaatga
     3121 cctgatgaga atggcaaaaa tatttcattt acttaattaa gtcaatactt ttccttaatt
     3181 caataaaatt taatttgttt aagttttgct taattattaa attaagcaga tttttttact
     3241 taatttatta taaaacaggt aatttaaact ataaatttgg attttactct ttaagaattt
     3301 aagttattca taccatgcgc agtgaatttt tctatgaaat acaacatgcc agagaaaaat
     3361 atctggacga attaagcaaa ctcaaagata aagagctcga tggacatgaa aacgacaaat
     3421 cactacagaa taagtccata gaaccccttt cttcgaacca taattactgt attcacgagg
     3481 attttgaaca gcggggttgc attccttcat taaatatttt cgcaaaactc atcaaggcca
     3541 tatatatagc cttcctttct gcaaaatata atcataatga ataagttaag acttttatgc
     3601 aagaattcat tctggtttgg tataattcaa ctaacttgaa cctatcgaat acgtggaata
     3661 attgattgat tataaatgat ttatcaatat aaaaggtttt gtatgtattt ttataatctt
     3721 attcgttcga tataagctca tatgagcgtt cagataagaa gaatgttaat taattacagt
     3781 atcctgatta tgaattttat ataatatatc tgttgaagta atggatgcaa cacttgtcga
     3841 ggaaataaaa cgttatattt caaccaatga ttcggcatct ctcataaaag caatgcataa
     3901 ctatcatttg gggatcgacg attttctcga cactgaaaaa tcggaaacag ccttgagtat
     3961 tgccattaaa ctgaaacaga aagatatggt tgcattttta cttcaatctg gtgccaatcc
     4021 taacctatgt gttcgatcgg aaacactatt gattttggcc atactcagcg atgagcccga
     4081 aatagtaaaa ctattactcg atcatgatgc cctatctgat gtaagcgacg ataatggaaa
     4141 tacgcctgtt gcgctttcca ttaaaagaaa tcaactgctt attttgacct atcttgtaaa
     4201 agctggggct aatctgcatg caaaagataa attcgggaag cggcctatag attacattca
     4261 ttcgtcgacc gatcaaaacc ttgcaatcta tctccaacat gctactttga tgcaagaaaa
     4321 agcactccat tggcctgaat ataccgacgg accctttgtg gagtggaaag attcgtcctc
     4381 ttttgagcta aagtatttct atactcgaga tcgtcgcttg tgtgaaaaat cggaattatt
     4441 ccattccgtt aaactccctt ttgtattaaa atcatccatc ctttttcatc ccgttgagat
     4501 aaaacgacat tatgatcgct caccgggtat ttgtgacatg cccgagcgct tactggccat
     4561 tggtgattta catggcaatt tacaatcact ccttagttta atgataggaa acaaaatcat
     4621 cgatgctgca ttcaattgga tatggggaaa tggtcaaatc gtgtttatcg gcgatttgtt
     4681 cgatagaggc tgccaagtta cggaactgat ttggttcgtt caacactttg aaagccaagc
     4741 acaaataaat ggcggacgag tccaccttat tttaggaaat cacgaaatta tggcacttat
     4801 tggcgatcat aggtacttga acgaaaaata tcgtaatatt tgtgaatatt tttcgatgaa
     4861 ctattgggaa ttttatactc ccgatagcga gttgggcaga tttatcagaa gcaaacctgc
     4921 aattttgaaa ataggcgata tactctttgt acatgctgga attgggccgg aacatctttc
     4981 taaaatgctg tcaataaatg aaataaatga tgtcatctat aatactttat cggataataa
     5041 acgtaaatta aatgaattag aaatatttat gctattagat gatggaccat tatggttcag
     5101 agatttactg catgaataca gttatttcca gactttgtct gagtcctaca ttgatagtgt
     5161 gcttagcttt tatgaagttt ctcatatcat tatagggcat tccgaaatac cagagatcaa
     5221 ctcgaggtac aatgggaaag tcattgctat caacgttcca ttttatcaca gcatgtatac
     5281 accgcaagct ttgcttattg aatgtaacca atattttatt atcggtgccg atggggaaaa
     5341 gaaacttttc ttaaagtagt tttgggttct tttcttaaaa gttaatttgt aatataattt
     5401 atattatgtt aaatagacta tttcattata tcgtcccctt atataaactc gtgattaaat
     5461 aaaacattcc ccctctttgt tttcgtcaga aaaaatgaaa ggagcaagaa tcaaagcaaa
     5521 gaaattcaga atgaagttaa tgcttattga accaatttat cagaaaagaa ttttgtttca
     5581 aaaatggcag aattttttct ttttccagaa aaacttcgat ggatccaaac gagtaaggtg
     5641 caatttcata aggttgataa acaaatccca aaccgttagt tgtaatatag atttcgttac
     5701 tcaattctat atgatcgctg aaaaatccag cttccaggag atttccgcgg gtttcttctg
     5761 cataagtttt tgctaaattt ttagtaatca aatcaatgat taggtcttgt ttctcaaggt
     5821 ctaactgttc ggcaagatgg attaccgaac cattttttaa attgaaagtc tgatatttcg
     5881 atacttccat tccatgagct cctcctgtaa aggcataatt taagattctg atacaaaata
     5941 tatctctact gttacacatt acttgaaatg aataagttct ttcccaatta aaagcagcat
     6001 cgggtaaaga tctatcataa tattcctgat tttctgtaat atatgattta caaaatttat
     6061 ctatttgatt atcaatggct tgtaagatag ttttaccttt tggatattct tgaccaaaat
     6121 agctcatgaa caaatgaaac atgatagaat catttattcg tggatttatg gaatactgag
     6181 gcaaagggaa aatgatatcg acaacggcct taggggtttc tttcatgcta aataatggtt
     6241 gcttcttgtt ttggtggata actttaagtg gtaagttacc gcgatcccat gtcaggttaa
     6301 aagaaggcag atacaactgt ttttgaccct ggtttaattt aggattcttc aattgttttt
     6361 gggtatataa aaaattcagc caaagttttt cgttcattga ttgaagagaa gcttcacccg
     6421 tatttttatc tatctttcca tagaaaatgg ttggggagtt gaagtctaat tctgctgatg
     6481 agggcatatc taggacgagt tgggcattat cattctctag aataatcaaa tcaacataac
     6541 atttcggaga tgagttatca ggtgtttcga aatgctgata gttttgggct tcgagatgaa
     6601 atcccgtaat taagttaacg ctaaacgcca taatggcaag aaataatttt ttcattgtcg
     6661 tagcctccat ttggttattt tggaaataag gtttctaaaa aacatataca tgaaaatgga
     6721 gaaagagagg ggtcattcga tgaatacccc tctcctattt tatgatagtt atttttggat
     6781 atttggaagt tggagaccat agccttttcg tttgtcttca gctttgagga gaaaaccgaa
     6841 aataagagct aaaacaccca gagtagtaaa gatgatcatg gggagagtat aattgtatgt
     6901 atttaccaaa gttccttcca ctaatttttg acctgtaacg caatatttat cgagtaccca
     6961 gccaattaat gcaggaacgc ccatcaagcc ccagttttga acccagaata tcaaggcata
     7021 ggctgtaccc agttgatgtt cggggatgat tttaggaacg gaaggccaca tagctgaagg
     7081 caccaaagag aagcctattc caagaacaat gattaaaagc acggcaatga tccattggtt
     7141 aatgaagggc aatgaaaata tggcatgtac aaagataagt aaaattgcac caataatcat
     7201 gattgtagca cctttccctt tacggtcgta tagattgcca aataaaggtg ttagcaggat
     7261 tgtgccaaaa ggtaatactg ctggaattgt gccggcgata gattgatcta ttccgaattt
     7321 atttaccatc atgtcggctg catatttaag gaaagggaaa actgccgaat agaagagcac
     7381 gcagagaata gcaataaacc accatccacg gttactgatg attttcagaa tatcagaaac
     7441 tttgaatgat tcttcgttgg ctactacctc attatctttg tcataggctt cttgagaagc
     7501 atcgagtttt ttatccatga aagtaaagat gaaaaaagag ataaaaccga tgcaaagcat
     7561 aatcaaacag ataagaatag gtttcgaaac atgacccaat ttttgggcaa taggtacgga
     7621 agtggacaaa gccaaagccg ttcctatgcg ggcagtagcc atttccaaac ccatagcaag
     7681 agctaattcc tttcctttga accattttac gatgattttc gatacggtaa ttccagccgt
     7741 ttccactcct actccaaatg ttgcatatcc taacgcagcc ataactacct gagctttgat
     7801 ctggaagagg ccaagaaaat tccaaacctg gccatcgagt gaatgggtcg atacagccca
     7861 gtatttgatg gccgtacccg taatcatgac tatagtagat gcaagccccg taaacctcac
     7921 gcccatttta tcgaggatga ttcctccaat gatgagcatg agtagaaaca cgttaaacca
     7981 gccataagca ctggtaaata tgccatattc agtactcgtc catgataact gctgctcgag
     8041 caaacctttt agtggagcca ttacatcggt gaggtagtag cccgttaaca tggtaaatgc
     8101 cacaagtgcc aaagcagtcc accgagctac ggctgaatcg cgcaaagtct gtcttacctt
     8161 ttccatcctt taatgtttag tggttgataa ttttatgaat tagttgcggc aaatttaaaa
     8221 tgaatcggca ttcaatgttc catgtatctt ccattttaac tttggctttc ggaatgtttt
     8281 catcatttgg ttgatttatt gatggatagt aaattcaaga attcctgaat tttttatctt
     8341 ctcttctgct gaatttccta cccacgatcc attggttgaa tgtttaaatt caattgattg
     8401 aggctaaaaa agaaaactca ggaaacaaaa aaacacacct catggaaaag atgtgttgtt
     8461 gtgactccgg caggattcaa acctgcaacc ttctgatccg tagtcagatg ctctattcag
     8521 ttaagctacg gagcctttgt ataatgcatt tgcaaaatta taaaattttt gaagaagata
     8581 tgatatatca ataagaattt tattttctaa gagtgattgt cattccatct tcagcaagat
     8641 aggtttcgga aaagatgttt cgagcttcgt tcagaaagct gtcgaggttg cgatatctcg
     8701 aagagatatg tcccaacatc aggcgtttag ccaaagcctt tttcgcaata gtagcagctt
     8761 gtatggttgt ggtatgccct ttttcggctg ctaattcggc cttgtcgtgc ataaatgttg
     8821 cttcatgata aagaaggtcg acatttttca atacttcggc tatgggagca taaaatgccg
     8881 tatcgaagca gtatgcaaac gaaatcggtg gatcgggaaa agtaatttct tcgttggaga
     8941 ggatacggcc atcgggcaaa acgtagtcct cacctgattt tatacgtact atatctgtat
     9001 aatgcggatg ataatcttcg ataaattgat gcaaaacttt tcgagggcgt tctttttcct
     9061 tgagtaaaaa gccataagct ggcaaacggt gttggagggg gaaggcagaa acggtaaaat
     9121 tatcttcttc acaaaccatt tcaagatttt gtgaacttaa gggatgaaaa acaagtggat
     9181 aattgagttc cttgacgctg attcccattt gaatcctaag aatctcttcg agcagaggag
     9241 gtccataaac atggagctca tttttgcgtt taccgatgtg ataggtaaaa agcaggccta
     9301 ctaatccaaa gaaatgatcg ccgtggagat gggaaataaa aatatgttgg atatgcaagg
     9361 cttttacttt gaaccgtttc atttgtattt gagttgcttc tccacaatcg aaaaggcaga
     9421 gtttttcgtc aacattggct acagtagcgc tcaatcctct tcgaagcgtt ggagtagcag
     9481 ctccagttcc caagaaagtt attgaaaaac ccatttttga attttacgta aaaaacaatt
     9541 ttcatttaaa aattcaccca aactcacata aaagaaagtt tgggtgaatt atacaaagta
     9601 tttcttccga tcaattctta tcagaaatct ctccagaggg ttcggaagta ttctcttctt
     9661 ttggattgct ggtttgttcc aaatcttcac ctgcagattg ttctatggct tctgcatttt
     9721 cttccttagg tttttggagt tcttctattg tttcggcaac agtttcttcg gaagattttt
     9781 tagtgatttc atcagaaggc agagcgggtg tttcatctga ggatggaggg acagcatgtt
     9841 cttctgtgtc atgagagagt tccggttctg gggtgagttc agaagtttta accttttctg
     9901 tttttttcga tttaggtgat ttttttcgtt tggataagtt ttcgagtttt gctttttcaa
     9961 cttccagcat ttcttcttta aggttggcga gggcttctat atcacccaaa gtactttttt
    10021 cgagggtttc ttgaattttc ttcaaagaag ccttggtgct cttaggggtt ttagctggct
    10081 tttcggtttc ttttgcttct ttggcttttt cggctgcctg agcatcctga taaaccttgc
    10141 tgtgtgaaac tacaattcgt ttattatcct tcgaaaactc ggtaactttt acatcgaggg
    10201 tttcatccag ttgggcttct tcgttatttt ctttaatggc ctgacgtgaa ggcaggtaag
    10261 cttcaactcc ataaggcaaa gaaacctcat atcctttgtc aactttttcg atgatggttc
    10321 ctttatgaat ggagcctggg atgaaaatgg tttcgaatac atcccatgga ttttcttcga
    10381 gctgtttatg cccaaggcta agcctacgtg attcaacatc tacgtctaag actaccactt
    10441 cgatttgttc gccaattttg gtaaactcgg ccgggtgttt tatttttttc gaccatgaaa
    10501 gatcgctgat gtggatcaaa ccgtctacac cttcttcgag ttcgacaaag atgccgaaat
    10561 tggtaaaatt gcgaacattg gcaatatgcc tcgaattgat aggatatttt tccttaatgt
    10621 tgatccaggg atcgggaata agctgtttaa tgcctaatga cattttgcgc tgttcgcgat
    10681 cgaggcttaa aataacggct tctacttcat ctccaacttt catgaaatca tgggcagtgc
    10741 gtaaatgctg cgaccacgac atttccgata catgaattaa accctcgact ccgggagcga
    10801 tttcgataaa tgcaccataa tctgcaatta caactacctt gccttttacc ttatcgccta
    10861 ctttcaggtt tggatccaaa gaatcccacg ggtgaggtgt aagttgtttc aatccaaggg
    10921 cgattctttt cttgttgtcg tcgaaatcga gaataaccac ttttattttt tgatcgagtt
    10981 gaactacttc ttcgggatga ttaattcttc cccatgaaag gtcggtaata tggatgaggc
    11041 catctactcc gcccaagtca acaaagacgc cataagtggt aatattttta actgtccctt
    11101 cgaggatctg acctttttcg agtttggcaa taatttcggc tttttgagct tcgagttcat
    11161 cttcgatgag ggctttgtga gaaacgacga cattcttata ttcctgattg atttttacca
    11221 ctttgaactc catcacctta tccacgaaaa catcataatc gcgtatgggt tttacatcga
    11281 tctgagatcc gggcaaaaag gcctcgattc caaatacatc aacgattagg cctcccttgg
    11341 tgcggcattt tacataccct ttaatgattt cctgattatc gtaagcctgg ttgacacgtt
    11401 cccatgcgcg taaaagcctg gccttcttat gcgaaagaat gagctgcccc gtactgtctt
    11461 cctgattttc gacataaact tccactttat cgcccatctt caagtcggga tcgtaccgaa
    11521 attccgatag aggaattact ccatccgatt taaaaccaat gttaacgact acttcacggg
    11581 gattcatggc cacaatcgtt ccttctacca cttcctgctc cacaacggtg ttaaaagtcc
    11641 ttccataaag ttcttcgagg cgattgagtt cttctttgct ataatgggct tttttgcccg
    11701 tgtcttccca gtcgaaatct tccattggga gaggcttctg tttcttttcc attcttgagg
    11761 gttttatcac agatacttta gactccacaa tgttttcagg gaaatttttt tgctcgggtt
    11821 cgactgttac tggagtttct tcctcaggag tagtttcctc ggttggttgc atgccaattt
    11881 cggcattttc ttcaaaatct gacattcaat tttaattgtg gttaaaccgg tgttaccctc
    11941 cggatggata aacccgtata acctggttag acataatttt taaacttcat ccggaaagaa
    12001 gctgcaaaat tagatatttt taacaaacta aatggattaa agttcgtaaa gagcggtttt
    12061 ttgattttag acgatttatt ttgcccaaga ttggcaatga gatattgaaa aatgagaagg
    12121 aatgaccttt gcaatatttg taaatttgcc cgtttttagt gatcaacatt attaaattcg
    12181 ataagaagaa tgaaattcag tcggtattca ttattttttc catctgtttt agtcattttt
    12241 ttggtctttt cttttttttc gtgcacaaaa gacgaaccct cgacagactc ctcctatcaa
    12301 ttgacttttt cgaacgattc ggttgttttc gatacggttt ttactaccat tggttctgta
    12361 actcaagaac ttagaatata caataagaat aataaaaaaa taaagatcag ttatctacgg
    12421 cttgcaggtg gagaacaatc ggcttatcgt ttaaacatag atggacaaaa tacctggata
    12481 aaaaatgacg tggaactcga caaagatgat agccttttta tttttgttcg tgttcggatt
    12541 aatccaaacg ataaaaattc tccctttcta gtaagcgatt cgattgaatt tctaaccaat
    12601 ggcaactatc aaaacatcaa acttatagct tatggacaaa atgcttatta tcacactcca
    12661 gatcattttc cggccgatct accaccttat tctatcatag aatgttctca aacgtggact
    12721 agcgacaaac cgcatctgat ctatggatgg gctatagtgg attcggcttc tacccttgtg
    12781 atggaaggcg ggacgagggt ttattttcat catggagccg gtttactcgt aaaaaaagaa
    12841 gctagcctaa aggtaaatgg tgaagccgaa aataaagtaa tttttagaaa cgacagactc
    12901 gatcaatttt acaaggattt gcccggtcaa tgggaaggga tttattttgc cgacggaagt
    12961 cctgaaagtt atatacatta cgctgagatt ctaaatggta caacaggact tatgatcgag
    13021 caaagaaaca acaatcgaag ggctcgattc gaaatcacag aaacccttat caggaacatg
    13081 aagaaaggtg gattaagaag cacgggttgt aatctatatt cagtgaactg cgtcatcgta
    13141 aattgtggtt cgtatgcact tcaactcgaa ggtggcattc acgaattcat tcatttatca
    13201 attggaaatt tctggacttc gggcatacgc atgtctcctt ccctattgtt gacaaattat
    13261 tacatggatg gtgaaaatca gataaatatc gaacccacaa gcttcaattt cacgaatagt
    13321 atcatttttg gaaatcagta cgaagagatc tcgatcgatc agggtgagca gggaactggc
    13381 ttcccctata ttttcgatca ttgtttactc aaaaccagtc tcaacacaac ggacgagagt
    13441 atatttcctt catgtctcat caatatcgac ccattatttg tagatataga aaagcccaaa
    13501 ctcgaacccg acagcttatc gcctgtactt cacgcaggta aatccgcagg agttctgcag
    13561 gatattttag gtcgccagcg agatatgcag aggccagctc ttggagctta cgagccagat
    13621 tattctgggg gtaagagata attatcagga gctaattatt cgatgaatta cattcgttcg
    13681 gggacgtcta ttcccagaag atgcatgcta atgcgaataa cctgggcagt aaattgtgaa
    13741 atagcaagac gggtaagcat gcgttgttca tcgggttcgt gcaatatgct caaattatga
    13801 tagaattgat tgtaacttcg tgctagttcg taggcaaaat tggctatttg agagggatcg
    13861 agttctttgg ccgattgctt gattactgaa gggaaatcat ataaaatctt caagatttct
    13921 ttttcttctt catagaatgt catattcttt tggggagtaa agctccacgg attggctttt
    13981 cggagtaccg agcggatgcg ggcatgagta tattggataa aaggtccggt attcccatca
    14041 aaatcgatcg attcggtggg attgaacaag atattcttcc gaggatcgac cttcagaata
    14101 aaatatttca gtgcagccag accaatcatg ctgaagagtt catgagcctc atattcgtca
    14161 aggtcggttg cttttccaag ttcgagggta atttcccggg cagtcaaaat tatttcctct
    14221 atcaaatcgt cggcatccac cactgttcct tcgcgagatt tcatttttcc ctcaggcaaa
    14281 tctaccattc cataagaaag atggaagatt ttgtcggcat aagttcggcc caaacgctgt
    14341 aggatgagtt ttagaacttc gaagtggtaa ttttgctcat tcccaaccac ataaatcatt
    14401 ttttgtggtg agaattcctg atatcgtaat tcagcacagc cgaggtcttg agtaatgtaa
    14461 accgaagtac catcggagcg aaggagcagt ttatggtcta aattatctga agttaaatcg
    14521 acccaaaccg agccatcttc tttttggtaa aaaacgcctg ctttaaatcc ttcttccaca
    14581 attttctttc ccaaagaata ggtttccgat tcataataaa ttttatcgaa tgaaatgccc
    14641 aggcgtttat atgtttcatt gaagccctca tacacccaag agttcatcat ttcccaaagt
    14701 gaatgcgttt cggcatctcc cgcttcccat tttcttagca tttcctgtgc ttcgagcatt
    14761 aatggagccg tgcgaagtgc cgtttcttcg tccaaaccgt ttgccttcag ctgattaact
    14821 tcttctttat attttttctc gaaaagaaca taataattac ctaccaggtg atcgccttta
    14881 aggccactgg attcaggagt ttcaccatta ctccattttt tccatgccag catggatttg
    14941 catacgtgaa tgccgcgatc gtttaccagg tttactctta ctacgggcaa acccgtagct
    15001 tcgaggattt cggcaaccga atgccccaaa agattgttcc gaatatgacc aagatgaaga
    15061 ggtttattgg tattgggcga tgagaattcg atcaatactg gtgcctgccc ttgtttttga
    15121 ggatttaaac caaaactgtg atttcgataa ttcttatcga gaaattccat ccagaaatca
    15181 tctttcagaa taatattgag ataacctttt attacgttga aactttcgat ttcgggaaga
    15241 gcatttttaa gccattcacc gatttcacga gcagtaagtt cgggagattt tttcgagatt
    15301 tttgcaaaag ggaaagttat aatagtaaaa tctcctgtga attctttttt tgttttattc
    15361 acggggactt ggttgggttg aacgatgtgg tgatataggg cttcgatagc atagcccgtt
    15421 tgttgagcaa gaagttgagc aaacatgtat aaaagcttgg agcgacaaaa ttatgaaaat
    15481 aggtcttatg gcattgtttt cttcccacat tcggccaact tccgattgat ctccatcata
    15541 aggtccccta agttttcggc cgcatcgacc agagatttat aataatcgac cgagagagga
    15601 ttaatctttc cctgttcaat ttttcttggg agtttttcct tgattttatc tctcatttta
    15661 ttgatttttt cttcaataag ttgagattcg gcaatattta ccttatgata ttctccattg
    15721 aggttattga caagcaattg gaaggccgaa tctacttcat ctaacaagcc catgaggtct
    15781 ctgtgttgtt tgggtgagaa ccatatcttt tctttcgatt tttcctgtat cgtttgcagc
    15841 aatttttggt tgacttttac aattacctga aaattttcca gtgtaaggag catggttcgg
    15901 taacgcacct gtccatcagc actcatttca tcgaaagaaa tggtttggat gtaatttaaa
    15961 atttcagtgt ggtggaattg aattttctga taaagacgct cagcatcttc attttgctga
    16021 atcagattcg aagtatctcc ggggacgacc gaagagatta cgaagcgaaa aagctctatt
    16081 acttcagaac ttaagacagc tatttcatgc tttaagtgta aaagattgaa ctcagaagtc
    16141 gaaataaaga aaggattgaa atatcttatt tgatatgggg tggaatattt tactggaata
    16201 atagtattta ctatagccga catttgagga atgaaattga tccagattgc cgtagtaatg
    16261 aggttcaaaa acgtatggaa ggctgagaga gcaaatggaa tggactgaga atcagaggac
    16321 ggattctgtc cggtaagtcc ttttgtaaaa cttgtcatca tttggatgat aaaagggaaa
    16381 aaagcaaggg ctaaaatcat caatgaaaca ttgaacaaaa aatgtgaaag tgctgcacgt
    16441 ctggccttgt aatttcccac aattgccgcc gcattagccg ttatggtagt tcctacattc
    16501 tcgccaatta ccatagctgc agcattttca taaccaacga ttcccgaagc acacagggca
    16561 aaagtaaaca ccagcatagc cgatgaagat tgcaaaacag cagtcaatac aaaacccatg
    16621 gtcacaaaaa aaagaataga gctaaatccc cgatgattgt gagattgtat ccaaccaatc
    16681 cattcaggat gagtagttag gctttggctt tgcatcatta aaaaattcat tcccataaac
    16741 aaaagggcaa aacccatgaa gaattctcct aaggccctaa tggtagcttt tttcgaaaat
    16801 agtagaatga aacataaggc aatgataggt agagcaaatt gagaaaggtc gaaattatag
    16861 ccaatgaatg caataatcca agctttgagg gtagtgccga tatttgctcc catgataagg
    16921 gaaaatgcct gagggaaagt caaaagattt gctgtacata atcctataat cattaccgta
    16981 gtagctgatg aggattggag tgctgcggtg attaccagac cagacagcac accctttacg
    17041 ggattctgag ccaaacccga aataaaattc ctcaggtttt gtccggccat cttctgcaaa
    17101 gcttcgctca tcagcttcat gccgaataga aacaagcata aggctccgat gatagacaat
    17161 atatttataa ccatcgaatg aaacggcttt gagaaatcag cctgcaaaac aagttaaaat
    17221 acagttaata aatgttttct aaaagaaaac ttaaagattt attaaactca tcctccatga
    17281 tgtgggttta tcaaatattc gagatgatct tctttgaatt ggagtaagga cgggaggatt
    17341 agatcggcca acacaaatcg caaatccatc gaggtttggt cagcaggaac ggccactgcc
    17401 ttcattctgg cagctttggc tgcaatgatg ccattgagcg catcttcgaa tacgatacaa
    17461 ttttgtggtt caacctgcaa ttgctcagca gtggtaaaaa atattccagg atgaggtttg
    17521 ccatattttt cttccgtggc actataaatg attttaaagt gctgatgaat gctcaatttt
    17581 cttagagcca gatcgataat atcgtagggt gaagccgaag caagagcaag aggaatatca
    17641 cgcgactgga agaaatgcaa tatatatacg caacccggta aacccctggc tttttcataa
    17701 atcgatcgtt ttacctcgga aataatttgt tgttcgatca agtcagggtc gagggcatca
    17761 aaagatgtac actggcaaaa aaatctaatt gtttcatcca cacgaaatcc tttcaccttc
    17821 tggcacattt gtgaagtaat ttttacccca agacgtgcaa atacgtttat ttcggcttct
    17881 tcccataagg gttccgaatc gatcaacagg ccatcgaggt cgaaaatagc tgcctgaaat
    17941 gagttcattc tgttattata agatgttttt taaactttcg agagcctttt caagaattgc
    18001 atgtggacag gcaatattca tgcgcataaa tcctttccct tcgattccga attcttctcc
    18061 ccggtttaat cctagatgag ccttatgaat caggtgtgca tgaagttcat cgtcaggaat
    18121 atgaagaccc cgaaaatcga gccacagcaa ataggtagct tcgggttgta cgaggtgaat
    18181 gggtgaaaga tgttcttcga agtaatgtgt tgctatcctc accgattgat gaagataatc
    18241 gagtaattct tcgagccagt cttcgccttg gttgtatgca gcttcgaatg caatattccc
    18301 taaaaggttt cccatttgca aatggaagcg ttcgagggtg tcgttgtaaa ttttgcgttt
    18361 ttgaggatca gggatcaaaa tggcactggt ggagagtccg gctatattga aagtcttgct
    18421 gggtgcaaag cagctagcag aacgcatgcc aatctccttg tccagcgaga gtaagggagt
    18481 atggcgatag cccggaaata taatatcgga atgaatctcg tccgaaacaa tcatgatatc
    18541 gttttgtata caaatctcgg aaagctgttc taattctcgt ctctcccatg cacgtccaac
    18601 aggattatgt ggattcgaca ggataagcat tctgacgcct ggggcttttt cgcgcaagtc
    18661 atcgaaatcc atcgaatatc tttccccatc gaatttcaat ggattgttta tgacaattcg
    18721 ttcgtgagct ctgggaactt cgaaaaatgg cggatagaca ggaggttgaa ctatgatttt
    18781 atcacctgga ttggtaaaag ccagagttag aaccataagg cctgtgacta ctcctggact
    18841 aaagctcaca atttggggat cgatagatag ggcatgacgg cggaataacc aattagcaag
    18901 gctttcattg aattcttgcg ctcgaaaaga ataacccaat aacggatgat tcaaacgccg
    18961 atgcaaggct tgcaaaataa attcgggggt agcaaaatcc atatcggcta cccacattgg
    19021 tactacctcc tcggtaccaa aaacttgatt gcgtaaatca tatttcacgc atgaggtacc
    19081 ttctcgtgga ataatcgaat cgaaattagc cattgtgaca taatgaatga aaacatgaga
    19141 gcaaaaaaac gctattttgc tctttaaaat tctggtttat tgttctccct tataaaaggg
    19201 tagttttact acttctgcct taataagttt tcctctgatt tcgataaaaa tctcacttcc
    19261 cggttgggat aattcggcag gcacatagcc cattccgatt gggatattga gcgaaggcga
    19321 catggttcca gaagtaactt ctccgatttt gtttccggca gcgttacaaa tggagtaatg
    19381 ttgacgggga attccttttt ctttcaatac aaaaccaact aatttgcgtt tcacgccttc
    19441 cttcttttgc tgaaggagat attctttatc gatgaagttc tttgtatcgg taaattttgt
    19501 aatccatccc agaccagctt ccagagggga agtggtatcg tcgatatcat tgccatatag
    19561 gcaaaatccc atttcgagtc tcaaagtgtc gcgagctccc aagccgatag gttttatccc
    19621 aaattcctta cctgcttcga aaacggcatt ccaaatcgta tcggctgctt cgttgggcat
    19681 ataaatttca catccgccac ttccagtata tcctgttgta gaaaacaaaa tatttttcac
    19741 tccggcaaat tcaagatttt tccatgaata atattccatg tcgagaatgg attcggaggt
    19801 gagtttttgc atggatttca tggccagtgg gccttgtatg gcaagctgcc cggtttcttc
    19861 ggaagcattg taaacatcag ttccaatttt cataccccta tctgaagcat gctgggtaat
    19921 ccaattccaa tccttctcca tattggctgc attaaccaca agaagataag attctgcagt
    19981 aattctgtaa accagaatat catccactat cccacctctt ccgttgggca tgcacgaata
    20041 ttgtatcttg ccatcgtaaa gttttgaaac atcattggaa gtaatatact gaagcagatc
    20101 gaaagcttta gggcctttca cccatatctc acccatgtga gatacatcga aaacacctac
    20161 gcttttcctt acggtaagat gttcggatat aactccttca tattgaatgg gcatattata
    20221 accagcaaaa ggcaccatct ttgctcctaa ggcaatgtga aattttgtaa aaggagtatt
    20281 tttcatgttt ttaaatattt taatatgttt tatagaattt tacaaaaaaa agaaaacgtt
    20341 atgcaaaggt ataaatagcc ttttcaataa atagactaaa aaataaattt ataggttgac
    20401 aatgacaggc cggtagccaa tatattggta aatgttccag aaagtattca ccaaaccatt
    20461 tggttttaac catttatagc gataactgga ttgaatgttg gatatacgca aataaggtag
    20521 gc
//
LOCUS       DAOORU010000021        19681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_3365_length_19681_cov_5.791501, whole genome shotgun sequence.
ACCESSION   DAOORU010000021 DAOORU010000000
VERSION     DAOORU010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19681
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3365_length_19681_cov_5.791501"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="PLF20_02700"
     CDS             <1..858
                     /locus_tag="PLF20_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOX79949.1"
                     /translation="PVNSIATTSSTLISHLSSLNILDHLIGVADTSYIFHPYIRQRIH
                     DRQVQEIARGDRIEAESLIACRPDLSFITLYAGQDYSRFQQMGLQIVPLADYMENHPL
                     GRAEWIRFVGYFVGKEKEADSIFRQIASEYSSVQKKVSHNDRPARIFDGFMNSGVWYV
                     SGGRSYMAQLYKDAGLSYVFSDLNETASKGLGFEEVFSRAHDADYWRIVVSQKENFSL
                     QDLLNLDKRYVLFESARKQKVVVCNASHVPLYEEGVAHPELILSDLVYLTRPELLPDY
                     IPRYYQIIE"
     gene            858..1907
                     /locus_tag="PLF20_02705"
     CDS             858..1907
                     /locus_tag="PLF20_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOX79950.1"
                     /translation="MHTLDISRHTPAFRIFIGIFLLLAFVILFFANLSVGTVKVSFSE
                     IMQIFAGKGRGLNQNALVVYEFRLPQALMAILAGASLAIGGLLMQALFRNPLADPSIL
                     GISSGASLGVALVTMAAGHISGVSIFSSGSWGYIGITLAALIGSLSVLMLLLFFSARL
                     KNIGQLLILGLMLGYLSYSLVSLLKFYSLKDDLQHYVIWGMGSFSEVSMEQLKIITPA
                     VMIGLVFSLLLAKSLNLMLLGDEYAQNLGLKLSLARTLIIFVAGFLTSIITAWCGPIA
                     FIGLAVPHLCRGLLATHDHHILLPVSMLTGAILSLICNFIARLPVFGGALPINAITSL
                     IGAPVMIGVILRLKN"
     gene            1921..2949
                     /locus_tag="PLF20_02710"
     CDS             1921..2949
                     /locus_tag="PLF20_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX79951.1"
                     /translation="MKFNRRVFPIHEDALTTNHLSIGYLNPKSVIASDINLNFRRGRL
                     YCIIGPNGSGKTTLLRTLSGFLKPLAGQITISGKILSQFSPSSLSTCISIVLTKQPAL
                     PLLTVYEVVAMGRQPYTGFWGHLTPSDKAVVHDVLQMVGIHCKAAEFFDHLSDGEKQK
                     VMIAKALAQQTPYIFLDEPAAFLDFPSRIEIMQLLANISHQQNKTILISTHDVPLAFR
                     QADEIILMATDKPIVSGVPNEIFASSLLEDYFEHNGKKFNPFSFDYGVEQTQGLPVFI
                     DVNEELHPLIAAFIKRRGFLPVAKAENSQHWLRIDANWEIRFSLYQQVYTFHNLEELD
                     LFLKNAKK"
     gene            3032..3697
                     /locus_tag="PLF20_02715"
     CDS             3032..3697
                     /locus_tag="PLF20_02715"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOX79952.1"
                     /translation="MDGDDNPATGCRVNGTNAEFLREFSLHDGLHRNFSAIIVGDNHN
                     IAACSIQTDLPYPDTLILIPVHLSCCSLDKERIAETQAIMYYLEKNYSVWRKEDTILR
                     LPVVITGDFNMVGSEIPYYNIITIQLTQAQKPWFTDLAPHQIDRPLAFTWRDYSKAFS
                     PNRLDFIFYSPLLNAVYSFVFHLLYLNENWLEKYGLNPLDLHFCSDHLQLVSDFALAA
                     SPN"
     gene            3710..5356
                     /locus_tag="PLF20_02720"
     CDS             3710..5356
                     /locus_tag="PLF20_02720"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOX79953.1"
                     /translation="MKKIFSLSIFPMIFLVLSSCHEQKQEADLIVIHANVYTLDEHFS
                     QQEAFAIKEGRILSTGKTKEILKKFSARDTLDLHGKTVYPGFMDAHCHFLGLGETMIR
                     WANLKGTRSMDEIIERLKSFIAENPQTWLLGRGWDQNLFPDNKFPDNSLLEKNFPNIP
                     VVIVRVDGHAALANDMALRLACISSDTKIDGGEILISQGKPTGLLLDNAKEKLMEAIP
                     AMDEQLLKHALQKAEAICISYGLTAVNDAGLPTHVIHTIDELQKNGVLKIRIVAMINP
                     DSLTISEFMHHGIYKTDRLLVNAIKLYADGALGSRGALLKSPYSDDPNTHGLLVESPQ
                     ILKEICLKAYNSDFQVCTHAIGDSANRLMLDIYSEILKGKNDRRWRIEHAQVVDPEDL
                     MKFGQFSIIPSVQPTHATSDMDWAEKRLGPQRLQYAYTYHDLLTQNGWIALGTDFPIE
                     EVNPIFTFYAATTRKNLQHRPPGGFLPQNKLSRRETLYGMTLWAAKSTFLENEIGSIE
                     PGKKADFIVTSDDLMQLPDDSIPHIVVEQTWINGKCVFHK"
     gene            5366..6304
                     /locus_tag="PLF20_02725"
     CDS             5366..6304
                     /locus_tag="PLF20_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79954.1"
                     /translation="MHRRLRTILHLLEQRKLTELQLEFDAILLEDHSEEVKYLYHLFR
                     QKLFTELTHEIQDVLQKTQHIVPSDINIQGIKTQIRILETQLSVVQYKKTEILKEIDR
                     FRIRHNSDLGWLIGKILKIQMEIFYLLQDVDSSYQANYQEAKKDYTAYQHQRRSLEKI
                     QPLQILDEKQRESMKKMYREASKLCHPDIVNDDFREEARKVFIELNRAYMINDIQKVE
                     EIHKLLTRSRMFSRLRTPSEERELLEARLARLQADLKILQSEIRELEESQVYHTLMNI
                     RDWDEYFEQTKMKLNAEYQFLKTEHEQLRKVRTEKK"
     gene            6267..>6957
                     /locus_tag="PLF20_02730"
     CDS             6267..>6957
                     /locus_tag="PLF20_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOX79955.1"
                     /translation="MSNLGKLERKRNEGIVKQASMLLDVTQKLIMKAEERNPSFMPMF
                     RGNYSRTGIAKTKSINQYPKVLYTFHELAGVYASPVMAEGKVYFGSDSHSFFCLDAQD
                     GSLIWQFKTGREIRSTAAINDGLLWFGSFDHHVYCLNANNGRMKWSFETKDWVFSSPA
                     VLNGILYISGIDGNIYALDAYSGKLKWNYETEGWVVASPAFTNGKLIVGSRDGWIYAL
                     DIHSGSLLWKTR"
     assembly_gap    6958..6967
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6968..7402
                     /locus_tag="PLF20_02735"
     CDS             <6968..7402
                     /locus_tag="PLF20_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOX79956.1"
                     /translation="AIHATAAIFKGHVFVGTLGNNFYCLNGDQGNIIWQETLASPIFS
                     SAAIDTNHLVFGSRSGILYSLCPLNGEILWQIRFDRAIHSSPVICGENVYFGCDDGKF
                     YCLQLSNGHIEWDYITRDVIRSSACIEEGKIWFGGLKLYCLG"
     gene            8401..8667
                     /locus_tag="PLF20_02740"
     CDS             8401..8667
                     /locus_tag="PLF20_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79957.1"
                     /translation="MCPGGAPGEVYIAAFDTGYCYSLFSSTDYGQHFSFRYRLPFLYI
                     NAIYRLGFQAGRAEGEFYIFIEYDQYYNGDITLREKIAREGVKD"
     gene            complement(8894..9472)
                     /locus_tag="PLF20_02745"
     CDS             complement(8894..9472)
                     /locus_tag="PLF20_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOX79958.1"
                     /translation="MKTCEALKLIEKYYPQDSEASRYYRIHVNAVAKAAELIVLNNPH
                     LNVDIEKVKVMALLHDIGIFMTDAPAIGCYGKYPYLAHGYLGRELLEKEGLLDIAPVC
                     ERHVGIGLSLTDIEKNLLPLPHRDMQPQTIEEKIVCYADKFFSKSSPRPEMPKPVEKI
                     INNLKKYGNDKIEKFLEMEKMFGSGYIYENFR"
     gene            complement(9517..10380)
                     /locus_tag="PLF20_02750"
     CDS             complement(9517..10380)
                     /locus_tag="PLF20_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79959.1"
                     /translation="MNKIKNLFFIVIILFYSSKIFSQQNIEYVQQADSLRKGWYFGIN
                     MGAAFPSNFSANFYNGSSENINKISLILDNQYYKPIIVQQIGYNYLFYELPHKMSYQP
                     NLTVGGYVRYQFSKRSSFLLQANYARLTASDVFLLYLEIPEGYSFDANYLQCQIMGQE
                     ERTYIDLGYRFDLRNHERYFPFIETGVNINNTKVLKNMIRIENQEYSIKYSGEHPIGP
                     YSQDYYDIYEGGIGVGGFFTLGYALNFNANISIDPSLTFYFTSTHLEGYTNMKPGFNL
                     LVRLMFRNFDF"
     gene            complement(10373..12454)
                     /locus_tag="PLF20_02755"
     CDS             complement(10373..12454)
                     /locus_tag="PLF20_02755"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOX79960.1"
                     /translation="MNLTTNIDRRAADNLRALIVAMVEKAKSGHPGGAMGAADFIHIL
                     YSEYLRFDPSDPNCIPNDPNWIARDRFFLDPGHLSALLYAVLTLCGKFSLDDLQNFRQ
                     LGGSTPGHPEKNIQRGIENTSGPLGQGHAMAVGAAIAERFLAARFGKVVEHKTIALIS
                     DGGIQEEISQGAGRLAGHLGLHNLIMFYDANNVQLSTKVDEVSSENTAMKYRAWGWKV
                     LEIDGNDPEEIRQALDAAWAETKSPTLIIGHTIMGKGIKNKEGKSMEGLVSTHGKPIS
                     KAGADVTITLRGLGADPQHPFAIFPDVEEHYLKVLNRKKEEARKAKETFDEWKEQNPK
                     LADELRQYFDKIQPEINWDAIHLSMNVATRVSSAEVLSVFSQTLGNMLVMSADLANSD
                     RTDGFLSHTQPIVLGDFNGAFLHVGVSELTMASLANGMALHGGVIPVCGTFMVFSDYM
                     KPAIRLAALMELRVIYVWSHDSFRVGEDGPTHQPVEQEAQIRLLEQLKNHSNQRSFIA
                     LRPADQAETLEAWKFALQNKNRPTGILLSRQNVKTLPPLPFERRRKEAVNLHHGAYLI
                     RKISDSPDIILLGNGSDVALLLEASDILFNQYQIKTNLISAPSEGLFREQSTEYQHSI
                     LPEEIPIFVLTSGLPSTFQTLAGSKGRIFGLEQFGYSAPAEILETHFGFTPQNIAKEV
                     MNLLDEINNNE"
     gene            complement(12720..14261)
                     /gene="rny"
                     /locus_tag="PLF20_02760"
     CDS             complement(12720..14261)
                     /gene="rny"
                     /locus_tag="PLF20_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOX79961.1"
                     /translation="MSTLYIILLVVIGMALASFITALAIRKNANSNANRILREAEEKA
                     EMLKKEKIVQAKEEILQFKLKNEQEIIEKNNSLLRLETRLKQREQQINQRSEELQKRQ
                     REVEAKNSMLNEKLEWVTQKETELEKALRQKTDILEKISGMTSKEARNQLIESMKEEA
                     RNDAITYIKDIMDEAKITAQNEAKKIVIESIQRTAVENAIENAVSVFNIESDEIKGRI
                     IGREGRNIRTLEALTGVEIIIDDSPDAILLSSFDPIRREIARLSLQKLVTDGRIHPAR
                     IEEVVAKTEKQIEQEIIEVGKRTVVELGIHGMHPELIRLIGKMKYRSSYGQNLLHHSK
                     EVSRLCANMAAELGLNAQKAKRAGLLHDIGKVAENESDLPHAILGMNLAEKYKEKPEI
                     CNAIGSHHEDIEMNTLIAPIIQVCDAISGARPGARREVVEAYMQRLHKLEELALSYPG
                     VVKTYAIQAGRELRVIVGADKVSDDEVNRISYELSRKIQEEMTYPGQIKITVIRETRV
                     INYAK"
     gene            complement(14509..14793)
                     /locus_tag="PLF20_02765"
     CDS             complement(14509..14793)
                     /locus_tag="PLF20_02765"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOX79962.1"
                     /translation="MDEISISLLIGERTYRLTIKKEEEETIRKVAHDINQRLQDYAQI
                     YAYRDKQDLLAMIVLELATQLEANKNLMEYHNLEIQNRIREIEGMIDQYI"
     gene            complement(14815..15123)
                     /locus_tag="PLF20_02770"
     CDS             complement(14815..15123)
                     /locus_tag="PLF20_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79963.1"
                     /translation="MPESETLDMLRSLRIKVEHFVEQYQVMVEKMKVIETINSDLLKK
                     NQEFQKHFEQMNNELKIQNLTELLTQGKGKTEVKAIIDRIVRDIDKCIGLINQPDLKE
                     "
     gene            complement(15196..15891)
                     /gene="trmD"
                     /locus_tag="PLF20_02775"
     CDS             complement(15196..15891)
                     /gene="trmD"
                     /locus_tag="PLF20_02775"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOX79964.1"
                     /translation="MRIDILTLFPGMFEGPFSESILKRATEKKLVEIHVHNIRDYSTD
                     KHRRVDDYPFGGDAGMVMMIEPIYKAIKTLKKERAYDRVIFTTPDGELFDQKMANYFS
                     TLQNIIILCGHYKGIDQRIRDHLIDQEVSIGNYVLTGGELAAAVMTDAIVRLIPGVLH
                     DETSALTDSFQDDLLSPPVYTRPCNFKGWRVPEILLSGDDGKIQQWKLEKAIERTKKR
                     RPDLLDEENTGKD"
     gene            16022..18994
                     /locus_tag="PLF20_02780"
     CDS             16022..18994
                     /locus_tag="PLF20_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOX79965.1"
                     /translation="MKFPIKILYAEDERLFREMMTDFLKDLAEEVHVADNGNSGMQLF
                     HKHGADLVVADIAMPGLSGLKMIRSIKREKPSIRSIITTSYEETSYFIDAIEIGVDKF
                     LLKPFDRDLLNKTIHELANIILNEQKLLKEEEARKRAEAELTESEARFRLLFEKAPES
                     IMVIDANTSKIVDMNASAVKLLGYDVNETRKMSLDTLMSDTSWSKATLEPNAKPYELD
                     LYSKFGESIPVEVSYNRISFTGQNLCLAIFSNLTERKKAEEALNQYKNKLELLVEERT
                     YQLQNTVEKLENEIQQRKAAQIDLAFKVQIEKVLSGISSSFIRLQDEFLEEEIIKSLS
                     QITSTAGMDQACLFILKENSSEIERKYIWSAPRHVKKVIFSDDFDLKDLEFAYSHLIK
                     HQAVVVNDRRLLTDNTREKKFMRLYHLQSFVFVPLVNEEKLAGVIGLASVSCAHINED
                     IVFMLKIAGKVFLDAQYRKDTTEKLIDSEEKARALLNVVPDAIVLLESNGNILDINEK
                     AAELIGGSTVNNQKPNYFDLLPEPIRTARQKIFKKVVSDGRRYSHYDTLLNRHYDLTF
                     YPASNLNKTIKRVAIFSRDITDILANQEAITNHNHFLRALLDNIPNPVFYKDTKGVFL
                     GCNNAFLQAFGKTSTDIIGKKICEIFSIENCRQIIQKDEEIIISKQIQSFELEFQYAD
                     GLIHNIIINQSPFSQSDGTLGGLVGIMIDISEIKQIQHELEQWNIQLNKRVSEELKKV
                     EKQRQMLIQKSKLESLGQLAAGITHEMNQPLTAITMGLENILFHLKSEDITKHYLENK
                     IGTILENIQRINRIIDHVKTFSRNQQNARFTRMNLNEAIQGALLLLKSQFSERNIHFN
                     LQLHPEPLYFLGDRFKVEQVVINLLNNSKDALEQKAHNQSLNFNKTIAIRSFIINSNN
                     IGMEIEDNGTGIPPQLLDHIFEPFFTTKEINKGTGLGLSIVYGIIEELKGNIEVKSEF
                     GEGTCFTCEFPSYMNTNN"
     gene            19065..>19681
                     /locus_tag="PLF20_02785"
     CDS             19065..>19681
                     /locus_tag="PLF20_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX79966.1"
                     /translation="MAEQLNILIVDDEEIIRNELAEYLQNNNFNVFLAEASTEALEVL
                     KANRVDVVFLDIRLPGINGLELLKIIKMEYPEKEVIMISGHGEMQSVIEAMRSGACDY
                     LPKPFSLTEVISAIERTRRFIDLNNRLKEVENSFNLFKDNIRKELGQGFIAQSQAMKK
                     VSELVSKVAKSDPTNVLIMGESGTGKELVARAIHYMSPRNDHYFYA"
BASE COUNT         5752 a         3910 c         3906 g         6103 t
ORIGIN      
        1 cctgtgaact caattgctac aacttcatcc accctcatct ctcatctttc ttctctgaat
       61 attttggacc atctcatcgg tgtagcggat acttcttata tctttcatcc ttatataagg
      121 caacgtatcc atgatagaca agttcaagaa attgctcgag gcgatcgtat cgaagccgaa
      181 tcactgattg cctgccgacc tgatttgtct tttattacgc tgtatgctgg tcaggattac
      241 agtcgttttc aacaaatggg gcttcaaatc gtgcctttag ccgattatat ggaaaatcac
      301 cccttaggaa gagccgaatg gattcgtttt gtcggatatt tcgtgggaaa ggaaaaggaa
      361 gccgacagca tctttcgtca aattgcctct gagtattcaa gtgttcagaa gaaagtttct
      421 cacaatgatc gacctgcgcg tatattcgat ggattcatga attctggggt atggtatgtt
      481 tcgggaggaa gaagttatat ggctcaatta tacaaagatg ccggtttgag ttatgttttt
      541 tccgacctga acgaaaccgc aagcaaaggc cttggtttcg aagaggtatt ctcaagagct
      601 catgatgctg attattggcg gatagtggtt agccagaaag aaaattttag cctgcaggat
      661 ttgttgaatc tcgataaacg ttatgttctt ttcgagtcag ctcgaaaaca aaaagtcgta
      721 gtttgtaatg cttcgcatgt gcctctatat gaagaaggag tagcccatcc cgaactgatt
      781 ctctccgatt tggtctatct tactcgacct gaacttctgc ctgattatat accccggtat
      841 tatcaaatca tcgagtaatg cataccttag acatctcacg tcatactccg gctttccgca
      901 tctttatcgg tatcttttta cttctggctt ttgtgatatt gttttttgct aatctttcgg
      961 tgggtacagt aaaagtaagt ttttcggaaa taatgcaaat ttttgcaggg aagggaagag
     1021 gtttgaatca gaatgctctg gtagtatatg aatttcgatt acctcaggct ttaatggcca
     1081 tattggcagg ggcttcactg gccattggag gcctacttat gcaggctttg ttccgcaatc
     1141 ctttggccga tccctccatt ctgggtatca gttctggagc aagtctgggt gtagccctcg
     1201 taactatggc agccggccat attagcggag tttccatatt ttcatcaggt tcatggggat
     1261 atattggcat tacgctggct gctctaatcg gctctttgag tgtgttgatg cttcttctat
     1321 ttttttcggc acgtctgaaa aacatcggcc aactgcttat tcttggactg atgttgggtt
     1381 atctgagcta ttcgttggtg agtttgctca aattttacag cctgaaagac gaccttcagc
     1441 attacgtgat ttggggaatg ggaagctttt cggaagtaag catggaacaa ttgaagataa
     1501 ttactccagc agtaatgatt ggacttgttt tttcgctttt attagcaaaa tctctcaacc
     1561 ttatgctctt aggagatgaa tatgcccaga accttggatt gaagctaagt ttagctcgta
     1621 cccttatcat atttgtggct ggatttttaa cctcgattat aactgcctgg tgtggtccta
     1681 tagcttttat cggattggct gttccacatc tttgtcgagg tcttctggca acgcacgacc
     1741 atcatatttt gttacctgtg agcatgctaa caggcgcgat tctttcttta atatgtaact
     1801 ttattgctcg tttgccagtg tttggaggtg cactgcccat taatgccatc acttcgctga
     1861 ttggcgctcc agtgatgatc ggggtgattc tgcgtttaaa aaattaaaat caatgtgtga
     1921 atgaaattca acagaagagt cttccccatt catgaagatg ctttaacaac gaatcatctt
     1981 agcattggat atttgaatcc aaagtctgta attgcgtcgg atatcaattt aaatttcagg
     2041 cggggtcgtt tgtattgtat tatcggaccc aatggttcag gcaagactac attgcttcgc
     2101 acgttgtcgg gtttcttgaa gcctctggcc ggccaaatca ccatttcggg caagatatta
     2161 tcgcaatttt cgccatcgag tctatcgact tgcatcagca ttgtactaac caaacaacct
     2221 gcactgcctt tgcttacagt ttacgaagta gtagctatgg gtcgccaacc atataccggc
     2281 ttttggggtc atcttacacc ttccgataaa gctgttgtgc acgatgtact ccagatggtg
     2341 ggtatccact gcaaggctgc cgaatttttt gaccacctca gtgatggaga aaagcaaaaa
     2401 gtaatgatag ccaaagcttt ggctcaacaa acgccctata tctttttaga tgaaccagcc
     2461 gcttttctcg atttcccgag ccgaatcgaa attatgcaac tgcttgcaaa catctctcat
     2521 cagcaaaaca aaaccattct aatttctacg catgacgtgc ctttggcttt tcggcaggcc
     2581 gatgaaatca tacttatggc caccgataaa cccatagttt cgggagtgcc caacgaaatt
     2641 ttcgccagtt ccctgctcga ggattatttc gagcacaacg gaaaaaaatt caaccctttt
     2701 tccttcgact atggggttga acaaactcaa gggcttcctg tttttatcga tgtaaatgaa
     2761 gaacttcatc ctctcatcgc ggcgttcatt aaacgaagag gatttctccc ggttgcgaaa
     2821 gccgaaaata gtcaacattg gctacgaatc gatgcgaact gggaaatacg attttctctt
     2881 tatcaacagg tatatacatt tcataattta gaagaactcg atttattttt gaaaaatgca
     2941 aaaaaataat cttctgagcg tttggctttt tgtcggttcg ttttttacca cttcctggct
     3001 tttttgtcaa agttatagaa acccgattat tatggatggc gatgataatc cagccacagg
     3061 ctgccgtgtg aatggaacaa acgctgagtt cttaagggaa tttagcttgc atgatggcct
     3121 tcatagaaat ttctctgcaa ttatcgttgg cgacaaccat aatattgccg cctgttccat
     3181 ccaaacagat ttgccttatc cggatacact tattttgatt cctgttcatc tttcctgttg
     3241 ttcattagac aaggaacgta tagccgaaac tcaagccatc atgtattatt tagaaaaaaa
     3301 ttattctgtt tggagaaagg aagatacgat attgcggctg ccagtcgtga taacaggaga
     3361 ttttaatatg gtgggctctg aaattcctta ctacaacatc attactatcc agctcaccca
     3421 agctcaaaag ccttggttta ccgatctggc accccatcaa atcgaccgac ctttagcctt
     3481 tacttggcgt gattattcca aggcattcag tcctaaccgc ctcgatttta ttttttactc
     3541 ccctttactc aatgcggttt attcctttgt ctttcacctt ctttatctga atgaaaattg
     3601 gcttgaaaag tatggtttga atccgctgga tttgcatttt tgttccgatc atcttcaatt
     3661 ggtcagcgat ttcgccttgg cagcttcacc caattaataa cttttgtcta tgaaaaaaat
     3721 cttttcttta tctatttttc ctatgatatt tctagtattg agtagttgcc acgagcaaaa
     3781 acaggaagcc gacctcattg tcattcatgc gaatgtttat acactcgatg aacatttcag
     3841 ccaacaggag gcctttgcca tcaaggaagg gcgaattcta tcaacaggca aaaccaaaga
     3901 gattctgaag aagttttcgg cccgtgatac actcgattta cacgggaaaa ccgtttaccc
     3961 aggctttatg gatgcacact gccatttttt gggattgggt gaaaccatga taagatgggc
     4021 taacttaaaa ggcactcgtt ccatggatga aatcatcgaa cgcttgaagt cgtttatagc
     4081 agaaaatcct caaacatggt tgctgggtag gggatgggac caaaatctct tccctgataa
     4141 taagtttccc gataattcct tacttgagaa aaattttccc aatatcccag tagttatagt
     4201 tcgggtggat ggtcatgcag ctctggccaa cgacatggca cttcgactcg cctgtatatc
     4261 ttccgatact aaaatagacg gtggagaaat tttaatttct cagggaaaac ccactggatt
     4321 gcttctggat aatgccaagg aaaaattgat ggaagccatc cccgccatgg atgaacaatt
     4381 gctgaaacat gccctgcaaa aggctgaagc tatttgcatt tcgtatggat tgactgcggt
     4441 aaatgacgca ggactgccga ctcatgtcat tcatacgata gatgaattac aaaaaaatgg
     4501 agttctaaag atcaggattg ttgccatgat caatcctgat tcgctcacaa tttctgaatt
     4561 tatgcatcat ggcatataca aaaccgatcg tttgttggta aacgctataa aactttatgc
     4621 cgatggagct ttgggcagtc gaggtgctct tttaaaaagt ccttattccg atgaccccaa
     4681 tactcatgga cttcttgttg aatctcccca aatactgaaa gaaatctgtc ttaaagcata
     4741 taattctgat tttcaggtgt gtacacatgc cattggcgat tcggccaacc gattgatgct
     4801 ggatatttat tccgaaatat tgaaaggtaa gaatgatcgt cgctggcgta tagagcatgc
     4861 tcaggtagtc gatcctgaag atttgatgaa atttggtcaa ttttccatca ttccttccgt
     4921 tcagccaact cacgcaactt ccgatatgga ttgggctgaa aaacgccttg gaccccaacg
     4981 cctccaatat gcttatactt accacgatct tctcacccaa aacggatgga tagctttggg
     5041 gaccgatttc cccatagaag aggtaaatcc catattcacc ttttatgcag ccactacccg
     5101 aaaaaatttg caacatcgac ctccaggcgg ttttttaccc caaaacaaac tctcacgacg
     5161 cgaaaccttg tatggcatga cgctttgggc tgcaaaatca acattcctcg aaaatgaaat
     5221 cggctcgata gaaccaggta aaaaagccga ttttatcgta acttccgatg atttgatgca
     5281 gcttcccgac gacagcatac ctcatattgt ggtagaacaa acttggatta atggtaaatg
     5341 tgtttttcat aaataacagc tgctgatgca ccgccgtctg cgaaccattt tacatttatt
     5401 ggaacagcgc aaattgaccg aactccagct cgagtttgat gccattctcc tcgaagatca
     5461 tagcgaagaa gtgaaatatc tttaccatct ctttcgtcaa aagctattca cagaattgac
     5521 ccatgagatt caagatgtat tgcaaaaaac ccagcatatt gttccttccg acattaacat
     5581 tcaaggaatt aaaactcaaa taagaattct tgaaacccag ctttcggtgg tccaatataa
     5641 gaaaacagaa atactcaagg agattgatcg gtttcgtatt cgtcataaca gtgacttggg
     5701 atggcttatc ggaaagattt tgaaaataca gatggagata ttttatttac ttcaggatgt
     5761 agattcctca tatcaggcaa attaccaaga agccaaaaag gattatactg cttatcagca
     5821 tcaaaggcgc agcttggaaa aaattcagcc tttacaaatt cttgatgaaa agcagcgcga
     5881 aagcatgaaa aaaatgtatc gtgaagccag taaactgtgt caccccgata tcgtgaacga
     5941 cgactttcgt gaagaagctc gaaaggtttt catagaactc aatcgcgcct atatgattaa
     6001 cgacattcaa aaagtagaag agatacataa attacttaca cggagcagga tgtttagtag
     6061 gctacgtact cctagtgaag aaagggaact tctggaggct cgtttagcac gcctgcaggc
     6121 cgatttaaaa attttgcagt ccgaaatccg tgaactcgaa gaaagtcaag tttatcatac
     6181 tctgatgaat attcgcgatt gggatgaata ttttgagcag acaaaaatga agttgaatgc
     6241 tgaatatcaa tttttgaaaa ccgaacatga gcaacttagg aaagttagaa cggaaaagaa
     6301 atgaaggaat cgtaaaacaa gccagcatgt tgttggatgt aacgcagaag ctcatcatga
     6361 aggccgaaga aaggaatcct tcgtttatgc ccatgttccg cggtaattat tccagaacag
     6421 gaattgccaa aacaaagtct ataaatcaat atccaaaagt cctttatacc tttcatgaac
     6481 ttgccggcgt ttatgccagt ccggtgatgg ccgaagggaa agtatatttc ggctccgaca
     6541 gccatagttt tttctgttta gatgcgcagg atggttcgct catctggcaa ttcaagacgg
     6601 gacgtgaaat aaggtcaacg gccgctataa acgatggttt gctttggttt ggcagtttcg
     6661 accatcatgt ctattgcctg aatgccaaca acggacgaat gaaatggagt ttcgaaacca
     6721 aagactgggt tttttcatcg cctgcagtct tgaatggtat cttatatatc tcaggaatag
     6781 atggaaatat ttatgcgctg gatgcttatt cgggaaaatt gaaatggaac tatgaaaccg
     6841 aaggatgggt agtggcatct ccagctttta ccaatggaaa attaattgtg ggaagccgtg
     6901 atggatggat ttatgcgttg gatatacaca gtggaagtct tctgtggaaa actcgatnnn
     6961 nnnnnnngcc attcatgcta cggcagctat tttcaaaggt catgtttttg tgggtacttt
     7021 gggtaataat ttttattgtt tgaatggaga ccaagggaat attatctggc aagaaactct
     7081 tgcttctccc atattctctt ccgctgctat cgatactaat catcttgttt ttggctcaag
     7141 atcaggaatt ttatattccc tctgtccttt gaatggagaa attttatggc aaatcagatt
     7201 tgatagagca attcattcct ctccagttat ttgtggagaa aatgtatatt ttggctgcga
     7261 cgatggaaaa ttctattgcc tgcagctttc taatggccat atagaatggg attatataac
     7321 ccgcgatgtt atccggagtt cggcctgcat agaagagggg aaaatatggt tcggtggact
     7381 taaactgtat tgtctgggat aaaatttcgt atcagttaac tctttaagcc taacacctct
     7441 ctaatatctg ttattcctaa atcaacaatt cttacatttt tggtaaaagt gtaattttta
     7501 gtattttagt aagactattt atatgattaa ctgaaaaata gaattttcaa tcagactact
     7561 aaattttatg taatgtttag ctgcaaaacg aaataataat gaaaccgtat gttggttata
     7621 ataaatatct ttaatctaaa ctgccataaa aagaatttgt ttacttttta cttctttaat
     7681 aatgggtata ttccccatct atagaatcta tagtcaggct gcatgggttc ctgttcccag
     7741 tagttatcca taacaaatct atcataagct gagggcacta aagggtttaa ataatttttc
     7801 atttaccttc agttcgagaa atttatcttt ctctgcaggt ctcaattttt aaaagttagt
     7861 actccaaatt atcaagtttg ttatccaatt gtaaaacttt aaaatcaaac aaccatgaaa
     7921 aaaatatttt tgttactttt tatcatttgt tattgtatga atcctctctt ttcgcaacat
     7981 tattcaaaag ggacggtgct gggggaattt tattatgtta caacatttat cacctataat
     8041 tttgggtggg aatatggttt aatatacagc aatgattatg gtcagtttat cagttattaa
     8101 aaagattcat ttgatcagaa tatttataaa gataagatta acggattaat ttattctcct
     8161 tgtaagataa ctggaggcac aagagttctc cgttccttat aacaatggag tagattttga
     8221 caccctgttt actcctaatg actttagttt tttatcgggt accattgaag gtgaagtaat
     8281 tggtctgaat ctgcctcaac cgccatacag cccgattgat ttatatgtaa gcagcgatac
     8341 aggcaatagt tttgaataca aaagtcagtt gccacattat aatttttcca tttatcctat
     8401 atgtgtccgg gtggtgcacc aggtgaagtg tacattgctg catttgatac gggttactgc
     8461 tacagccttt tcagcagtac ggattatggg cagcattttt cattcagata ccggctacct
     8521 tttttgtata tcaacgcgat ttaccgttta ggttttcagg ctggcagagc agaaggagag
     8581 ttttatattt ttatcgaata tgatcaatat tataacggcg acattacttt gcgagaaaaa
     8641 atagcacgcg aaggggtaaa ggattgaatc aatttttctt agcgttcttg gcgtctttgt
     8701 gagaaaaaat agcacacgaa gcagagaatt gcatcaaatt tttttaatgt atgtaaaaag
     8761 agtatttgta attgacagga tcgatatgcg atggcaggat aaagcgtgta agatttttta
     8821 tcacattttt ggcaacaatg attttttatt gccaaatttg tgataattta gtttgaagtt
     8881 ttatataaaa attttaacga aaattttcgt aaatatatcc agagccaaac attttttcca
     8941 tttctaagaa tttttcaatc ttatcattac catatttttt cagattattg ataatttttt
     9001 cgacaggttt gggcatttcg gggcggggcg aagacttcga aaaaaattta tcggcataac
     9061 aaacaatttt ttcctctatt gtctgtggct gcatatcacg atggggcaga ggcaataggt
     9121 tcttctcgat atcggttaac gacaagccta tgccaacatg tcgctcgcaa acaggcgcta
     9181 tatcgagtaa accttccttt tctaaaagtt ctcttcctaa atatccatgt gccaggtagg
     9241 ggtatttccc gtaacaccca atagccggag catcggtcat aaaaatccct atgtcgtgta
     9301 acagcgccat aacctttact ttttcaatgt cgacatttaa gtgagggtta tttagtacaa
     9361 tcaattcagc agcctttgcc acggcattta catgaatacg ataataccga ctggcttccg
     9421 aatcttgagg ataatacttc tcgatgagtt tcaaagcttc gcatgttttc ataactcgca
     9481 cttttcttca attcaacaat atttttccca atgtgtttaa aaatcgaaat ttctgaacat
     9541 caatctgaca agaagattga agccaggttt catattcgta taaccttcaa gatgggtgct
     9601 tgtaaaataa aaagttaaac tgggatcaat tgaaatattt gcattgaaat tcaatgcata
     9661 acctaatgta aagaatcctc cgactcctat accaccttca taaatatcgt aataatcttg
     9721 cgaataaggc ccaataggat gttctccaga atattttatg ctgtattcct gattctcgat
     9781 gcgaatcatg ttctttaaca ctttggtatt attgatattc acacctgtct ctataaaagg
     9841 gaaatatctt tcatgatttc tcaggtcgaa ccgatagccc agatcaatgt aagttctttc
     9901 ttcctgtccc atgatttggc attggaggta gttggcatcg aaagaataac cttcgggaat
     9961 ttcaagataa agaagaaaca catcgctggc tgttaatcgc gcataattgg cctgtaaaag
    10021 aaaagatgat cttttcgaaa attgataacg cacatatccg cctaccgtaa gattaggctg
    10081 ataagacatt ttatgtggca attcatagaa aagataattg tatccaattt gttgtacgat
    10141 tataggttta taatattgat tatcgagaat taatgatatt ttattgatat tttcggaact
    10201 tccgttgtaa aaattggccg aaaaattaga agggaaagca gcacccatat taatgccaaa
    10261 ataccagccc ttgcgtaaac tgtcggcctg ttgcacatat tcgatattct gttgtgaaaa
    10321 aattttactt gaataaaata aaataataac aataaaaaat aggtttttta ttttattcat
    10381 tgttatttat ttcgtcgagt aaattcatca cttcttttgc aatattttga ggagtgaatc
    10441 caaaatgagt ctctaggatt tcagcgggtg ccgaatatcc gaattgttcg agtccaaata
    10501 ttctgccttt tgatccggca agggtttgaa aggtagatgg caagccagag gtaagcacaa
    10561 agatcggaat ttcttcaggc aaaatagaat gttgatattc tgtggattgt tcacgaaata
    10621 agccttcgct gggggcagaa atcaggtttg tttttatttg atattgatta aaaagaatat
    10681 cgcttgcctc caaaagcaaa gccacatcag agccatttcc caaaaggatg atatcgggtg
    10741 agtcgcttat tttacgaatc agataagcac catgatgaag atttacggct tctttacgac
    10801 gtctttcgaa aggcagaggg ggtaaggttt taacgttttg ccggcttaac agaatgcccg
    10861 tggggcgatt tttattttga agggcaaatt tccatgcttc gagcgtttct gcctgatcgg
    10921 ccggacgaag agctatgaaa cttctctggt tagaatggtt ttttaattgt tccaacaagc
    10981 gaatctgtgc ttcctgttcc acgggttgat gagtcggacc atcttctccc acacgaaatg
    11041 aatcgtgcga ccaaacatag atgacccgaa gttccattaa ggccgccaag cgaatggctg
    11101 gcttcatata gtcggaaaaa accataaaag tgccacatac aggaattaca cctccatgca
    11161 gagccattcc gttggccaat gaagccatgg tgagttccga taccccaaca tgcagaaagg
    11221 ccccattgaa atctcccagc acaataggtt gggtatgact aagaaatccg tcggttcgat
    11281 cactattagc aaggtcggcc gacattacca gcatatttcc aagggtttgg ctaaaaacag
    11341 agagtacctc ggccgagctg acacgagtag ctacattcat gctcaaatgt atcgcatccc
    11401 agtttatttc gggttgaatc ttatcaaagt attgtctaag ctcatcagcc agtttgggat
    11461 tttgttcttt ccattcgtca aaggtttcct tcgctttgcg ggcttcctct tttttcctgt
    11521 ttaaaacttt tagataatgt tcttcaacgt cgggaaaaat ggcaaaagga tgctgaggat
    11581 cggctccgag tcctctcaaa gtaatcgtaa catcggctcc ggctttgctg ataggctttc
    11641 catgtgtaga tacaagtcct tccatggatt ttccttcctt atttttgatt ccttttccca
    11701 tgatggtatg gccaataata agggtagggg acttagtttc agcccaagca gcatccaatg
    11761 cttgacgtat ttcttcgggg tcattcccgt ctatctcgag cactttccaa ccccaagccc
    11821 gatatttcat ggccgtattt tccgatgaaa cttcatctac ttttgtcgaa agctgaacat
    11881 tattggcatc ataaaacatg ataaggttat gcaatcccag atgcccggcc aagcgacctg
    11941 ctccttgtga aatttcttcc tgtataccgc cgtccgaaat aagagcaatg gttttatgct
    12001 ctaccacttt tccgaatcgt gcggctaaaa atcgttcggc aatagctgct cccacggcca
    12061 tggcatgccc ttgtcccaaa ggacccgagg tgttttcgat gcctctttgt atattctttt
    12121 ctggatggcc tggcgtactg cctcctaatt ggcgaaaatt ttgcaaatcg tcgagtgaga
    12181 attttccgca taaggttaga acagcataca acaaggctga caagtggcca gggtcgagga
    12241 agaaacgatc ccgagcaatc caattcggat cgttcggaat acaattggga tcggaaggat
    12301 cgaaacgcag atattcacta taaagaatat ggataaaatc ggctgctccc atcgctccgc
    12361 ccggatggcc agatttggct ttttcgacca tggcaacaat aagagcccgc aaattgtcgg
    12421 ccgcccgcct gtctatattg gttgtcagat tcatcgaggt tttattttcg tgattgaaag
    12481 acacgggcaa aattagaaat tttgattctt aaaatcctaa aatttgtcgc ccgtagcggg
    12541 ttgggaaaat ataggggaaa tagagacgac aagagagagg aatgaatggg aaaactgtat
    12601 tttttgcaat ctacaagatt agcacgaaag aggattttag ttctccaatt tctcagtctt
    12661 aaccgttgca tgcaatttat ctatcaatca agcaaacagt caatagagaa ataagatgat
    12721 tactttgcgt aattgatgac ccgggtttct cgaataacag tgattttgat ttgccctgga
    12781 taggtcattt cctcttgaat ttttcgtgag agttcataag aaatgcggtt aacctcatcg
    12841 tcagaaacct tatcggctcc cacaatgact ctcaactcac gtccagcttg aattgcgtaa
    12901 gtttttacca caccagggta ggagagggca agttcttcaa gtttatgcag ccgttgcatg
    12961 taggcctcaa ccacttctcg acgagctcct ggtcttgcac ccgaaattgc atcacagacc
    13021 tgtataatag gagcaatcag tgtattcatt tctatatctt catgatgaga accgatggca
    13081 ttgcatattt cgggtttttc cttgtatttc tcggcaaggt tcattcctag aatggcatgt
    13141 ggcaggtcgc tttcattttc ggctactttg ccaatatcgt gaagtaaacc tgcacgtttg
    13201 gccttttgag cattcagtcc tagttcggca gccatatttg cacataaacg tgaaacttcc
    13261 ttcgagtgat gtaaaagatt ctgcccataa gaggaacggt atttcatttt gcctatcagg
    13321 cgaatgagtt caggatgcat tccatgaatc cccagttcaa ccactgtacg tttgcccact
    13381 tcgatgattt cctgttcgat ctgtttttcg gttttagcta ctacttcttc aattcttgct
    13441 gggtgaatac gaccatcagt aactaatttt tgcagcgata aacgagctat ctctctacgt
    13501 atgggatcga aactcgataa aagaatggca tcaggggaat cgtcgatgat gatttctacg
    13561 ccagtgagag cttcgagtgt tcggatattt cttccttcgc ggcctatgat gcggcctttg
    13621 atctcatcac tttcgatatt gaaaaccgaa acggcatttt caatagcatt ttcgactgct
    13681 gttcgttgga tcgactcaat gacaattttc ttggcttcgt tttgagcagt gattttagcc
    13741 tcgtccatga tgtccttaat atatgtaata gcatcgtttc gggcttcttc cttcatgctt
    13801 tcgatgagtt gattacgagc ctctttactg gtcatgcccg aaatcttttc gagtatgtcg
    13861 gttttttgac gtaaggcttt ttctaattcg gtttcctttt gggtaaccca ttccaatttt
    13921 tcattaagca tagaattttt tgcttctacc tctctttgtc gtttctggag ttcctcagaa
    13981 cgctggttta tttgctgttc acgctgcttc aaacgtgttt ccaaacgaag cagactgttg
    14041 tttttctcga tgatttcctg ttcgtttttc aatttaaatt gtaggatttc ttctttggct
    14101 tgaacgattt tttctttctt gagcatttcc gctttttctt cggcttcgcg aagaatgcga
    14161 ttggcgtttg agtttgcatt ctttcgaata gcaagtgcag taatgaaact ggccagtgcc
    14221 atacctatta ctaccaataa gataatatat agcgtactca tgcagatagg tggtttttaa
    14281 acggaaaaga aaaacccgca aacaattcct ggagttttga aaccccattc tataggttta
    14341 gggttgccct gcttctttaa acttccactg ttatcctgaa aggaactccg ccgagcggag
    14401 tcgtggcctg tttgccaaaa cagaaagact ccccttaagt gtttaatgtt gagtttgcaa
    14461 accgaatgga attgctgcgg gtaatatcat ttggatgtca aagaacgttc aaatatactg
    14521 gtctatcata ccttctattt ccctgattct gttttgtatt tcgagattat ggtattccat
    14581 gaggttttta tttgcttcta actgagtggc aagctccaga actatcattg caagcaaatc
    14641 ctgtttatcg cgatatgcgt aaatttgcgc ataatcttgc aacctctggt tgatatcatg
    14701 ggctacttta cgaatggttt cttcttcctc ctttttaata gttaaacgat atgttctttc
    14761 tcctataagt agggatattg agatttcatc cattgttgaa tgtcagttct cgacttactc
    14821 cttcaggtca ggttggttga ttaaaccaat acatttgtca atgtcccgca caatcctatc
    14881 gataatggct ttgacttctg tttttccttt tccttgggtt aataattcgg ttaagttttg
    14941 tattttaagt tcgttgttca tctgctcgaa atgcttttga aattcctgat tcttttttag
    15001 cagatcggaa ttgatggttt ctatgacctt catcttttcg accattacct gatattgctc
    15061 cacaaaatgt tctactttga ttcgaagaga acgaagcata tcaagtgttt cgctttcagg
    15121 catttcggtt taaacatttc gatacgatgt acactttgca aagttaatat aaatcttaaa
    15181 aattatagcc ctattttaat cttttccggt attttcttcg tcgagcaagt caggacgtcg
    15241 ttttttagtc cgctcaatag ccttctcgag cttccattgc tgaatttttc catcgtcgcc
    15301 cgataagaga atttccggta ctctccatcc tttaaaattg caaggacgcg tataaaccgg
    15361 cggcgaaagc aaatcatcct gaaacgaatc ggtaagtgcc gaagtttcgt cgtgcaaaac
    15421 tcccggaatc agtctgacga tggcatcggt cattactgca gcagcaagct ctccaccagt
    15481 caatacataa tttcctatcg aaacttcctg gtcgatcaaa tgatctcgta tcctttgatc
    15541 gattcccttg taatgcccac ataaaatgat gatgttctgc aaggtcgaaa aataattggc
    15601 catcttttga tcgaaaagtt caccatcggg tgtagtaaaa atgaccctgt cgtatgctct
    15661 ttccttcttc aaagttttta ttgctttgta aattggttcg atcatcatta ccatacccgc
    15721 atcaccccca aagggataat catccacacg ccggtgttta tcagtcgagt agtctcgtat
    15781 gttgtgtaca tgaatttcta ccaacttttt ttcggttgct cttttgagaa tggattcgga
    15841 gaaaggccct tcgaacattc cgggaaataa ggttagaata tctattcgca taaaaaaatt
    15901 tttcgaaaaa taaccataat tggaatgaaa gttttatttt tgtaaaaatt ttgctctttt
    15961 agtagaacct gcaaatttat caaacccact catatatgac gaaataattg aaatttagct
    16021 cgtgaagttc cctataaaaa tattgtatgc agaagatgag cgtttgtttc gtgagatgat
    16081 gaccgatttt ctgaaggatt tggccgaaga agttcatgtg gccgataatg gaaattcggg
    16141 tatgcaacta tttcataaac acggagccga cttagtagta gccgatattg ccatgcctgg
    16201 cctaagcggc ttaaagatga ttcgaagcat aaaaagagaa aaaccttcca taagatctat
    16261 cattaccacc tcctacgaag aaacgagtta ttttatcgac gccattgaaa taggagtgga
    16321 taagttttta ttgaaacctt tcgataggga tttgttgaac aaaaccattc atgaattagc
    16381 aaacatcata ttgaatgagc agaagctgct gaaggaagaa gaagcccgta agcgagccga
    16441 agctgaactt accgaaagcg aggctcgttt caggctatta ttcgaaaaag ctcccgaaag
    16501 catcatggtg atcgatgcaa atacatccaa aattgtggat atgaatgctt cagcagtaaa
    16561 gctactgggt tacgatgtca atgaaacccg taaaatgtct ctcgataccc ttatgtcgga
    16621 tacttcatgg tcgaaagcga ctcttgaacc taatgccaaa ccatatgaat tggatcttta
    16681 ttcgaaattt ggagaatcta tacccgttga agtttcctac aataggattt catttactgg
    16741 ccaaaatctt tgtcttgcca tctttagtaa tcttaccgag cggaaaaaag ccgaagaagc
    16801 cctcaatcag tataaaaaca aactcgaact tctggtcgag gaacgtacct accaactgca
    16861 aaataccgta gaaaaactgg aaaatgagat tcaacaacgc aaagctgctc aaatcgatct
    16921 ggcatttaaa gtacagatag agaaagttct ttcgggaata tcgagttcat tcataagact
    16981 tcaagacgag tttttagaag aagaaatcat aaaaagcctt tctcaaatta cttcgactgc
    17041 aggaatggat caagcctgtc tgtttatttt aaaggagaat tcttccgaaa tagaacgaaa
    17101 atatatctgg agcgcacctc ggcacgtcaa aaaagtgata ttctctgacg acttcgattt
    17161 gaaagatctc gagtttgcat atagtcatct aataaaacat caagctgtcg tagtgaatga
    17221 tagaagacta ttgacagaca atacgaggga aaagaaattc atgcgacttt accatttgca
    17281 atcctttgtg ttcgtacctt tggtaaatga agaaaaatta gccggagtta ttggactggc
    17341 ttcagtttcc tgtgcgcata tcaacgagga tattgtattt atgctaaaaa tcgctggtaa
    17401 ggttttttta gatgcacaat accgaaagga taccaccgaa aaattgatcg acagtgaaga
    17461 aaaagcccgg gcattattga atgtcgtacc cgatgcaatt gttttactcg aatctaatgg
    17521 taatatactc gatattaatg aaaaagcagc tgaattgatc ggtggttcca ccgtaaataa
    17581 tcaaaaacca aattacttcg accttcttcc cgaacctatc agaacggccc gtcagaagat
    17641 tttcaaaaag gttgtaagcg atggaagaag atattctcat tacgataccc tgttgaaccg
    17701 acattatgat cttacctttt accctgcatc aaatctcaat aaaaccatta aaagagttgc
    17761 tattttcagt agagatatta ctgatatact tgcaaatcaa gaagctatta ccaaccataa
    17821 tcatttctta cgtgctcttt tggacaatat ccctaatcct gtgttctata aagatactaa
    17881 aggggttttt ctgggatgca acaatgcttt cttacaagcc ttcggaaaaa cttcaaccga
    17941 tattatagga aagaagattt gtgaaatttt ttcgatagaa aattgccgcc aaattatcca
    18001 aaaagatgag gaaataatta tttcgaagca aatccagagc tttgaactgg aatttcaata
    18061 tgctgatgga cttatacaca atataattat caatcaatct cccttttccc aaagcgatgg
    18121 tactttaggg ggcttggttg gaatcatgat cgatatcagt gaaataaagc agatacagca
    18181 cgaactcgaa caatggaata tacaattgaa caagagggtt agcgaagagc ttaagaaagt
    18241 cgaaaaacag aggcaaatgc ttattcagaa atccaaactc gaatcattgg gtcagctcgc
    18301 agctggaatt actcatgaaa tgaatcagcc ccttactgcc ataaccatgg gactcgaaaa
    18361 tattcttttc cacttgaaat cagaagacat cacaaagcac tatctcgaaa acaaaatagg
    18421 aaccatctta gagaatattc aacgcatcaa ccgcataatc gaccacgtaa aaaccttttc
    18481 acgcaaccaa caaaatgccc ggtttacccg catgaatctg aatgaggcca tacagggagc
    18541 cttgttgcta ttgaagagtc aattttccga gcgaaatatt cattttaatc tgcaacttca
    18601 tccagaacct ttatattttt tgggcgaccg cttcaaagtg gaacaagtcg tcatcaattt
    18661 gcttaacaat tcgaaagatg ctttggaaca aaaagcccat aaccaaagcc ttaattttaa
    18721 taaaacaatt gccattcgca gctttataat aaactccaat aacataggaa tggaaatcga
    18781 agataatggt acaggcattc cacctcaact gcttgatcat atcttcgaac cctttttcac
    18841 aactaaagaa atcaataagg gtacaggatt aggtttatcc atcgtttatg gtattataga
    18901 ggaattaaag ggaaacatcg aagttaaaag cgaatttggg gaaggcacat gctttacctg
    18961 tgaatttcct tcttacatga ataccaacaa ttgataagcc agtcggaatt tataactttg
    19021 caaaaattcc ttctcataga acattaaaac gaatttgctt aagaatggct gagcaactaa
    19081 atattcttat tgttgacgat gaagagatta ttcgtaacga attagccgag tatcttcaaa
    19141 ataacaattt caatgttttt ttggccgaag cctccaccga agccttggag gtattgaagg
    19201 ccaatcgcgt tgatgttgtt tttctcgata ttcgtttgcc aggtataaat gggctcgaac
    19261 ttttgaaaat cataaagatg gaatatcctg aaaaagaagt gattatgatt tcgggccatg
    19321 gggaaatgca aagcgttatc gaagctatgc gaagcggagc atgcgattat ttgcccaaac
    19381 ctttttcgtt gaccgaagta atttcggcta tcgaacgaac gcgccgattt atcgatttga
    19441 acaaccgact caaagaagtt gaaaattcat tcaatctatt taaagacaac attaggaaag
    19501 aacttggaca aggttttatt gcgcagagtc aggccatgaa aaaggtatct gaacttgtaa
    19561 gtaaagttgc taagagcgat cctaccaatg tgttgatcat gggtgaaagc ggcacaggca
    19621 aagaactagt cgccagagct atacactaca tgagccctcg caatgatcat tacttctatg
    19681 c
//
LOCUS       DAOORU010000022        19367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_3437_length_19367_cov_5.426315, whole genome shotgun sequence.
ACCESSION   DAOORU010000022 DAOORU010000000
VERSION     DAOORU010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3437_length_19367_cov_5.426315"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLF20_02790"
     CDS             <1..309
                     /locus_tag="PLF20_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOX79967.1"
                     /translation="LGQGRVWSGVDAKNIGLIDDYGGLQYAIDEAARMAGIENYKVIE
                     YPETKHPLTQIMESFGKSKMQKAMKNQVGPYFNHFITWQNLTQIKGPIARLPYDIYFE
                     "
     gene            394..939
                     /locus_tag="PLF20_02795"
     CDS             394..939
                     /locus_tag="PLF20_02795"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOX79968.1"
                     /translation="MNALEEEKQQIRKFIKACKAKLTIEEIRLCSQLILKNIESLPAF
                     QKAETLMAYWALPDEVQNHDFILRWYQHKKIILPAVDGSHLRLKVFSGINNLAQGQCF
                     GIEEPIGEDFAHPEKIELVIVPGIAFDISNNRLGRGKAYYDKFLRSLQTLKIGICFPF
                     QLLPRIPVGPDDIKMDMVISC"
     gene            986..2086
                     /locus_tag="PLF20_02800"
     CDS             986..2086
                     /locus_tag="PLF20_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HOX79969.1"
                     /translation="MYSKEQILTALRQVIDPDLKNDIVTLGMVENLRIEENKVGFQLV
                     LTTPACPLKGKFQKECIEAIHQKVDPNLIVEVEMISRVTSTRKKAVESLSGVKNIIAI
                     ASGKGGVGKSTIAANLAVALARTGASTGLLDADIYGPSQVMMFGLNNIRPGVTQLNGR
                     DLIEPVEKYDVKILSIGFFVDPDKALIWRGPMASGALTQLFTDANWGVLDYLVIDMPP
                     GTGDIHLTLVQQLAITGVAVVTTPQEVALADARKALDMYTQEKINVPILGIIENMSYF
                     TPAELPNNKYYIFGKEGGRRFSERYKVPLLGKIPLVQSICESSDKGNPIALDVDSPEG
                     KAFDLLAANIARAVAIRNDHLPPTHIVEIFGD"
     gene            complement(2352..2567)
                     /locus_tag="PLF20_02805"
     CDS             complement(2352..2567)
                     /locus_tag="PLF20_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOX79970.1"
                     /translation="MSIGVIFAGAIGQTELIIIIAVILLLFGGKKIPELMRGLGKGVR
                     SFKDGMSGKEDEEISNPGNKGDKPEKS"
     gene            complement(2618..3871)
                     /locus_tag="PLF20_02810"
     CDS             complement(2618..3871)
                     /locus_tag="PLF20_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HOX79971.1"
                     /translation="MSITEIHLSDFIYDLPQQKIAMFPAEPRDSSRLLVYNPNNDFLA
                     EDIFENVIEYLPHDSLLIFNETRVVHARMIFRKPTGGQIEVLCLNPVSPSSEIETAFQ
                     QTSPVIWKAYVGNAKRWKSGVLKMDFEYGNQHRGIFQVRQVGRKEGIFFLEFSWEPEE
                     LTFSEVLEAAGKIPLPPYIRRKAIPADDIRYQTVYARCDGSVAAPTAGLHFTEEILQH
                     LPKKDIQPLNLILHIGAGTFKPVDQDDVRQHVMHTEEIIVKCETIERLLISPEKLRIV
                     VGTTGVRTLESLYQTGKKLLQGIPLHRAVHTNQWDPYEGNLPETSMALEAILDALDKE
                     GQDAFRGTTSLMIVPGYQFKFTDILITNFHQPRSTLLMLVAAFCGNSWRKAYNYALDH
                     QFRFLSYGDACLFFKNNVQLNGIAV"
     gene            4834..5550
                     /gene="pssA"
                     /locus_tag="PLF20_02815"
     CDS             4834..5550
                     /gene="pssA"
                     /locus_tag="PLF20_02815"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOX79972.1"
                     /translation="MASIRKNIPNLLTSFNLLSGVIGLALLIRGNLPFASMMVLIAGI
                     FDFLDGMVARLLSVKSLLGKELDSLADVVSFGVLPGFILYHIVISQNLLWPLIAFPPL
                     LIPVFAALRLAKFNIDRRQEEEFRGLPTPAMAIFIASLEAPYGFSTLFLNSILSAFLF
                     HPLTLSLLALLLCFMMISEIPLFSLKMKSFSFKEYPYPMVFLCVSLMLVLIFKTAGLG
                     FSILTYVFLSLVKLLFRKRE"
     gene            complement(5557..6282)
                     /gene="lptB"
                     /locus_tag="PLF20_02820"
     CDS             complement(5557..6282)
                     /gene="lptB"
                     /locus_tag="PLF20_02820"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOX79973.1"
                     /translation="MKLHTQGLYKKYRNRMVVKDVSVEVNQKEIVGLLGPNGAGKTTT
                     FYIIVGLIKPFAGKVFIDDQEITSFPMYKRAQLGVGYLAQEASVFRNLSVEDNIKAVL
                     EFSHFSKNEQKKRLEELLTEFGLNHIRKSKGIQLSGGERRRTEIARALAVDPKFILLD
                     EPFAGIDPIAVEDLQNIVAHLKDKNIGVLITDHNVHETLSITDRAYLLYEGSVMKSGT
                     SEELANDEQVRRVYLGQNFELRR"
     gene            complement(6279..6635)
                     /locus_tag="PLF20_02825"
     CDS             complement(6279..6635)
                     /locus_tag="PLF20_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOX79974.1"
                     /translation="MKDNVDDFKEYRQRMNEKILAAPNNIVLKRIYSVDAMAYKDGAL
                     NAKTKEMLGLATSMVLRCDDCIRYHLIQCHQLGVTTEELLELFGIVNLIGGTIVIPHT
                     RRAFEFWEELNPQNHL"
     gene            complement(6657..7619)
                     /locus_tag="PLF20_02830"
     CDS             complement(6657..7619)
                     /locus_tag="PLF20_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOX79975.1"
                     /translation="MEKENIRKIAEQTLHEEALAIQGLKQHIDDEFVHAIEVILEASG
                     RVIITGIGKSSDIGRKFTATLNSTGTPAFFLHAADAIHGDLGMVRPDDIVVCLSKSGD
                     TPEIKVLLPLIKANGNTLIGIVGNPQSYLGLQSDIVLNVHVEHEACPNNLVPTASTAA
                     QLAMCDALAVALLTARGFTIEDFARLHPGGALGKKLYLRVSDLYLRNEKPQVETHTPI
                     TQVIFEISSKRLGCTAVLDQDRLIGIITDGDLRRMLQKNIELKNIVASDVMTTSPKTI
                     LPSSLVADALDVMRSNNITQLLVTENGIYLGVIHLHDILKEGVV"
     gene            8182..10401
                     /gene="recQ"
                     /locus_tag="PLF20_02835"
     CDS             8182..10401
                     /gene="recQ"
                     /locus_tag="PLF20_02835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HOX79976.1"
                     /translation="MKVELREKVVVKDPLYRALKEVFGFESFKGSQEVIIRNLMAGKD
                     TFVIMPTGSGKSLCYQLPAIISEGTAIVVSPLIALMKNQVDAIRNFGMEIGLAHYLNS
                     SLSRQELAQVKDDLAAGRTKLLYVAPESLTKDDNIELFKSIKLSFYAIDEAHCISEWG
                     HDFRPEYRRIRPMIEAIGQQVPVMALTATATPKVQQDIQKNLAMMNATLFKTSFNRPN
                     LYYEVRPKTENVIKDIIKYIKSQPGKSGIVYCLSRKKVEEVAETLQVNGIRAIPYHAG
                     LDPQTRRENQDSFLMEDADVVVATIAFGMGIDKPDIRYVIHHDIPKSLEGYYQETGRA
                     GRDGGEGNCLAFYSYDDILKLEKFLKNKHVAEQEITKLLLSETVAYAETTICRRKVLL
                     HYFGEQYTQDNCGNCDNCLHPKTRYDGKEAVLTVLDAITQTKQQCKSKHLVHVIIGKL
                     TANVKAAKHHKLEVFGRGEEYDEPYWHSVVRQMLVENLIEKDIENYGILRITNEGKKF
                     LKKPKTIMLTQDREYDNNEDDELIDQNTGAKPGTADATLFALLKDLRKEISRKENLPP
                     FVIFQDPSLEEMAIQYPITLDELKQITGVGTGKASKFGKPFIDLIKNYVEENEIIRPN
                     DMVIKSVINKSGLKIYIIQSIDRKVPLEDIALAKNLSFDELLTEIEHIVASGTKIDIS
                     YYINEYIDEYHQEEVFEYFRTAETDSIEKAREELGEEEFSEEEIRLMRIKFMSEMGN"
     gene            10593..11213
                     /locus_tag="PLF20_02840"
     CDS             10593..11213
                     /locus_tag="PLF20_02840"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOX79977.1"
                     /translation="MKNKFNLSLILSLLSLFGVIVLFILYFTHRPLGREGIPKAAETG
                     GHRIAFFRTDSILHHYDFVKERAEELAQKSRKYGEDLHRRQSEFENEAAYFQESVRKN
                     ALSEKSAQEIYQELMQKQQSIIDLKDRYDMELAGEEAQINLILFDSVTNLLKRFNKSY
                     GYDYILGYNKNGNVFYTNDTFDITQMVIKDLNREYRAVKGKTSGSK"
     gene            11228..11593
                     /locus_tag="PLF20_02845"
     CDS             11228..11593
                     /locus_tag="PLF20_02845"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HOX79978.1"
                     /translation="MKKDKCLFLMITVLLFIGCIQPKEKKPGALITPRKMAEILTDLQ
                     IADAWIAIKQNEGQDIKLLSTQIEDSLFKKHHITRAQFDTSMVWYSAHAEELDKIYDQ
                     VIQQLELLKEQNKTSKKQN"
     gene            complement(11590..12033)
                     /locus_tag="PLF20_02850"
     CDS             complement(11590..12033)
                     /locus_tag="PLF20_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79979.1"
                     /translation="MKSHSLLGQFRLKNIEFVWIVIFSLMFVLQACEDIEISPDGDDE
                     RTPWLGQWICTESPNKATQNAYIVRIMIDPDNSAQVKLYNYFQLGDDVAPFAIVTSST
                     ITVPQQQVAGGSWTVSGFGIMKNNVIEWSSYKANELSFKATFRRL"
     gene            complement(12040..12573)
                     /locus_tag="PLF20_02855"
     CDS             complement(12040..12573)
                     /locus_tag="PLF20_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79980.1"
                     /translation="MDRDRNFAATTHLNNSTSSVFPILLVDFAAIAFICFMPRMVKIT
                     GIPFYLIDPMRIWVLTALVFTNKPNTFILALGLPLLSHLAGGHPHIVKTLLLSTELLI
                     NVSLLYYLEKKWKLLWVVLVSIIISKSIYYSMKFASHQFGWIEGELLSTPLWIQAVMT
                     ILLSFYVTLFYQRKIKK"
     gene            complement(12582..13406)
                     /gene="deoC"
                     /locus_tag="PLF20_02860"
     CDS             complement(12582..13406)
                     /gene="deoC"
                     /locus_tag="PLF20_02860"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOX79981.1"
                     /translation="MKPLNIYPFSEEENKERIEEIKTIASTKEKDAHLLKQLYSFIDL
                     TSLGGKDNRQSIGNLCLLALLLSNKVAAVCVYPVFTRFVKSLLKDSGIRTVAVAGAFP
                     SGQSPFSIRLAEVEWTLQEGANEIDIVINRGKFLEGKTKEVFDEIKAIVDVAKEIPVK
                     VILETGELESTRNIRKAGLLAMEAGAAFLKTSTGKITPAATPEAALVMCDTILEFYHS
                     TGKKIGFKPAGGIQTPSQAILYYTLVEQVLGPEWLKPQLFRIGASHLVKALIESNI"
     gene            complement(13469..14782)
                     /gene="rseP"
                     /locus_tag="PLF20_02865"
     CDS             complement(13469..14782)
                     /gene="rseP"
                     /locus_tag="PLF20_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOX79982.1"
                     /translation="MNILIMTGQLILGLSILVILHELGHFLAARAFKIRVEKFYLFFD
                     AGNFKLFSFKKGETEYGVGWLPLGGYVKIAGMIDESMDKEQLKSPPQPWEFRSKPAWQ
                     RLIVMLAGVFVNLILGILIFGFTLLHYEKSYLPLNEINKDGIYASAKGKEIGFEAGDK
                     ILRINGKPVERFKDVHSVDLLFGSTFEVERNGKIITIEIPEDFYRREASSRGVRFIEP
                     LNFPFRIDSVFPGLPAANAGLTKSDKILKIDSIEIKSYGQFKELITNSAGRLVAIQTL
                     RGNDTLNMQLTVDSSGIIGILASIPYAQAPYSFGKALSYGYKDAMDMLSANIKGLGKI
                     ISGEEKASDSLQGPIGIAQIYGSQWIWAKFWYITGLLSLILAFMNILPIPALDGGHVL
                     FLLIEIVTRRKPSEKVLEYAQIAGMIILLGIMVFAIGNDIMRILR"
     gene            complement(14846..15997)
                     /locus_tag="PLF20_02870"
     CDS             complement(14846..15997)
                     /locus_tag="PLF20_02870"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007214906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOX79983.1"
                     /translation="MNPKHLAILGCTGSIGRQCLDVVREHPQSFVVEILAAQGFSKAL
                     VSQAKEFRPKVVAVAKSSDFEVLRQAFEQDHIDVAAGENALAEVVAMPEIDMVVSAIV
                     GFAGLRSVIAAIEAKKHIALANKETLVVAGEWVMNRLKSLQVDLLPIDSEHSAIFQCL
                     EGEKPNKVEKIILTGSGGPFRGMSLERLQNVKPEDALHHPTWNMGPKISVDSATLMNK
                     GLEVIEARWLFHLNPNQIEVIIHPQSVIHSMVQFVDGSIKAQMSIPDMRLPIQYALSY
                     PERLPSSFQRFDFIRNSPLTFEAPNTHVFRNLILAFEALHQGGNMPCILNAANEIAVE
                     AFLQHRIPFLRMPVIIEKCMNMIDFISSPDLSQLIETDRITREVAQGFL"
     gene            complement(15997..16878)
                     /locus_tag="PLF20_02875"
     CDS             complement(15997..16878)
                     /locus_tag="PLF20_02875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOX79984.1"
                     /translation="MHINVKNIRTVLFRNIKSKYRLVIQDAETFEEKFSFKLSRLNVF
                     ITLGILIIGLIFLTTYLIAFTSLKEYIPGYSNPDLSRQVYELTLRADSLELLLDQRAT
                     YLENIQRVLTGNVPLRKAADSTIQKHDYSKIDDTRIPEDSILRKEVENQDKYGLKPGY
                     STSIIKSSYISSFNFFRPVNGYLTSHFDPGHGHFGVDIATAEGETVKAILDGTVIQAG
                     WNIETGYTITIQHISNVVSIYMHNSSLLRKQGDIVKAGEPIAIAGSTGVLSTGTHLHF
                     ELWYNGTAIDPKNYIAF"
     gene            17479..>19367
                     /locus_tag="PLF20_02880"
     CDS             17479..>19367
                     /locus_tag="PLF20_02880"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX79985.1"
                     /translation="MKSKNFIPKGIIIFLWIVTIIIPTGCSNKKNTFSRRVYHNLTAH
                     YNTWWNGNESLKEAIKDLEKNAKDNYTEVLPVYKLGSKKEATAINSKADRAIEKASKV
                     IQNNSMYFNKKEYNRWIDDSYMMIGKAYFYKQDYASARRTFEFILTRYKDSPLIPEIY
                     LWLGLTNNQMEQFSRSEAELEKFRMLSARKTMPRSMMLFYNKVLADFYLKQNRTEEAI
                     KTLHIATDLSRKKSDKTRLHFILGQLYMGKNDLIKAFQMFQEVIKRTPPYDMQFAAQL
                     NLARCFDVASGEAEKLEKMLQNMLKEEKNKFYQDQIYYALAEIALKKGDRTAAIENLR
                     LSVAKSKDNDFQRVQSSLTLADLYFEKPDYFCSQAYYDTALQALPKDHPTYEALQKKS
                     QVLTELVSNLTVVQTEDSLQRLAQIPENERLKIIDGIIAKYAEEEKKKQEEEMLRQSD
                     LTMLGQGNIQRQMDPSGAWYFYNPSAISLGFTEFKRKWGNRKLEDLWRLSNKQITTAW
                     EEEQTMAFSDSVANDSLLVKISTDPLKRQTYLQNLPLTPEKLRQSNQNIIEALFNAAF
                     IYREGLLDMVNAEKTFEELVERFPDTSLNKHYLLGCYQLVVINEKLENQSKADHYRNI
                     LITNYP"
BASE COUNT         5875 a         3855 c         3920 g         5717 t
ORIGIN      
        1 ctcggacaag gaagagtatg gagtggagta gatgccaaaa acattggatt gatcgatgat
       61 tatggaggtc ttcaatatgc catagatgaa gccgcccgta tggcaggtat cgaaaactac
      121 aaagtgatag aatatcctga gaccaaacat cctttaacac aaattatgga atcatttggc
      181 aaaagcaaaa tgcagaaagc catgaaaaat caagtaggtc cttacttcaa tcattttatt
      241 acatggcaaa atttaactca aataaaaggc cctattgctc gtttacctta tgatatttat
      301 ttcgaatgat catcgacaaa aattatattg accaacaacc atacgaaata ataaatcctt
      361 aattcctcat agtttcgtct tttaaaaaga tttatgaatg cactcgaaga agaaaaacaa
      421 cagataagaa aattcattaa agcctgtaag gcaaaattaa ccatagaaga aatccgactt
      481 tgcagtcaac ttattttaaa aaatattgag tcattgcctg cattccaaaa agctgaaaca
      541 ttgatggcat attgggctct gcccgatgaa gtgcaaaacc atgattttat tcttcgatgg
      601 tatcagcaca aaaaaatcat tctacctgca gtagatggtt ctcatctgag gctaaaagta
      661 tttagcggaa taaacaattt agctcaaggt caatgctttg gcattgaaga acccattggc
      721 gaagactttg cacatcccga aaaaatcgag cttgtcatcg ttccaggaat agcgttcgat
      781 atctcaaaca atcgccttgg acgtgggaag gcttattatg acaaatttct tcgatctttg
      841 caaactctta agattggcat ttgctttcct tttcaattat tgcctcgaat tccggtagga
      901 cctgatgata ttaagatgga tatggtaatt tcatgctgaa ttcagcctaa atctgattaa
      961 gaatgattaa atcaaataaa gttttatgta ttcaaaagaa caaattctca ccgccttacg
     1021 tcaggtaatt gatcctgatc ttaaaaatga tattgtaacc ctcggaatgg ttgaaaacct
     1081 ccgaattgaa gaaaataaag ttggatttca actggttttg acaactccag cctgtccatt
     1141 gaaagggaaa tttcagaaag aatgtatcga agcaattcat caaaaggtag atcccaattt
     1201 gatagttgaa gtcgagatga tttcgagggt aacgagtacg cgtaaaaaag ccgtagaatc
     1261 tttgtcgggt gtaaagaata tcatagccat tgcatcaggg aaaggtggtg tgggcaaaag
     1321 tacgatagca gccaatctgg cagtagctct ggctcgtaca ggagcaagca ccggtcttct
     1381 tgatgccgac atttatggcc cttcacaagt gatgatgttt ggattgaata atataagacc
     1441 tggtgttaca cagctcaatg gacgtgattt gatcgagcct gtcgaaaaat acgacgttaa
     1501 aattctttcc attggttttt ttgttgaccc cgataaggca ttgatctgga gaggacctat
     1561 ggcctcggga gcccttacgc agttattcac tgatgcaaat tggggcgtac ttgattatct
     1621 ggttattgat atgccccctg gaactggcga tatccattta accctcgtac agcaattggc
     1681 tataacgggg gtagctgtgg ttactactcc tcaggaagtt gccctggccg atgcccgaaa
     1741 agccctcgat atgtacaccc aagaaaagat caatgtcccc attttgggaa ttatcgaaaa
     1801 tatgtcgtat tttacaccag ccgaactacc caataataaa tactacattt ttggaaaaga
     1861 gggtggacga aggttctctg aacgttataa ggtacccctc ttgggtaaaa ttcccttagt
     1921 tcaatccatt tgtgaatcga gcgacaaagg caatccgatt gcattagatg tggattcacc
     1981 cgaagggaaa gcctttgacc tgctcgctgc caatatagca agggccgtgg ctatccgaaa
     2041 cgatcatctt ccgcctacac atatcgtcga aatatttggt gattaatagt tgttgaatga
     2101 aaagtgttat gcaatgcgta ttgtgtgata cactttgcag cgcaggagcc gtgcggcgtg
     2161 agaagagctt ggaactaagt ttcgagaaga attttagggt ttgatatggg gagactggga
     2221 gacaaagagg atgtgataat ggggaaaaat caggaccagc aaatgaaaga ccccgccaaa
     2281 acgtctatct ctggcagatt agactaatag aaattttatt cagaggaagg gggaatagtt
     2341 gcccgaagaa atcaggattt ctcgggttta tcgcctttat tccctggatt agaaatctct
     2401 tcgtcttctt ttccggacat accatctttg aaacttctaa cccctttgcc caatcctcgc
     2461 atcaattcag gaatcttttt acctccaaaa agaagcaata tgacggcaat gatgataatg
     2521 agctcggttt gtccgatggc acccgcaaaa ataaccccta tgctcatgat attgattttt
     2581 atttcgttaa tgcaaaggta taggaatttt gctttaatca tactgctatt ccattaagtt
     2641 gcacattgtt tttgaaaaat agacaggcat ctccataact caagaaacgg aactggtggt
     2701 cgagcgcata attataggcc tttctccatg aatttccaca aaatgcagct accaacatta
     2761 aaagggtact acgcggctga tgaaaatttg ttatcaagat atcggtgaat ttgaattgat
     2821 atccgggaac aatcatcaag gaagtggttc cacgaaatgc atcttgacct tccttatcga
     2881 gagcatcaag aatcgcctct aaagccatag aagtttcggg caaattacct tcgtatggat
     2941 cccattgatt tgtatgtacc gctcgatgca aggggattcc ttgtaagagt tttttgcctg
     3001 tttgataaag gctttccaat gttctaacgc ctgtggttcc cacgactatc cggagctttt
     3061 ctggtgaaat tagcagcctt tcgatggttt cgcacttcac gataatttcc tcggtgtgca
     3121 tcacatgttg tcgtacatca tcctgatcaa ctggtttaaa agtgcctgct cctatatgca
     3181 gaatcaaatt cagaggctga atatcttttt tggggagatg ttgaaggatt tcttccgtaa
     3241 aatgcaaacc agcagttggt gccgccaccg atccatcaca acgtgcataa acggtttgat
     3301 atcttatatc atcagcgggt atagctttgc gacgaatgta tggtgggagg ggaatcttgc
     3361 ctgctgcttc taaaacttcc gaaaaagtta actcctcagg ctcccatgaa aactcaagga
     3421 aaaagatgcc ctcttttctt cctacttgcc taacctgaaa gatgcctctg tgttgatttc
     3481 catattcgaa atccattttc aatactcccg atttccagcg ttttgcattg ccaacatacg
     3541 ccttccatat cactggcgaa gtttgttgaa aagcagtctc gatttctgag gaaggactta
     3601 ctggattcag acataatact tcgatttgcc cacccgttgg ttttcgaaag atcatcctgg
     3661 catgtactac acgagtctcg ttaaaaatta gcaaactatc gtgtgggaga tattctataa
     3721 cgttttcgaa aatatcttcg gcaagaaagt cattgttggg attatatacc agcagacgtg
     3781 aactatcgcg gggttcggca ggaaacattg ctatcttctg ctgtggtaaa tcatatatga
     3841 aatcgctgag atgtatttct gtaatcgaca ttcatttatt ccatttagac tatataaaaa
     3901 gctttataaa ggacttcaac aactttgcga ccaataatgt atttcaaaat catatcagat
     3961 tggaacaatt acatacatat agcttcgatt ctcttgagcc gagaaataaa cctgactttc
     4021 attgattcga ttatcctttg atttcgtagc tgtggtttat agcttctcca gcacaaataa
     4081 aaattttgtg caaaaataca ttctacactc gaatctccat tcggcttatt gtcgtaactt
     4141 ttctctacgg ctatatttcg gatttttgat tcttaatttt aaatccgatt caattcgcgt
     4201 aagttcaaat ccctgtgaca aattaatctt caattcttaa tttgtttacg taagccctca
     4261 taaaaaaata cagtttactg tgagaattta acccataacc gtttcaaagg tgctataagg
     4321 gagattgtct cttggaaggg atatcagttt ttcaaatttc tctcaacgtc agggatttta
     4381 cgtgaaaaaa tagtaagtcg gagtgaagtc aaacgtgatg cagattgaga tgaatttttc
     4441 gagccgggga aaatgtttta ttcttacaag gctgcagtat ggttttactt tgatgtgttt
     4501 atgctactaa aaactctggg tttggaaagg tgagaggatc tcgggaacca ggtaatgaga
     4561 agtaaaattc gtataatagt tgatgagctt ttctttttta tgattcagcc catgctttga
     4621 aacagactca tattttcata gccttgggat tttcgatgtt tgttacgaat tatattattt
     4681 cgattccaac ataatacttt tttgaattcg aagaatgctt ccttacgaag cagattatga
     4741 ataggatgag aatagcttgc ctcatatcga attcatagaa tgacgcagca aattatttga
     4801 aacctcagta cttttgccac attattttaa tttatggcaa gtattcggaa gaacataccc
     4861 aatttgctca ctagtttcaa tcttttatca ggggtgatag gattggcttt gttaataagg
     4921 gggaacttac cttttgcttc gatgatggtc ctaattgccg gcatctttga ttttttggat
     4981 ggaatggtgg cgcgcttatt gagtgtgaaa tccctgttag gcaaagagct agattccttg
     5041 gccgatgtag tttcatttgg cgtgcttccg ggatttattc tgtaccatat agtaatttca
     5101 caaaatctac tttggccgct gatagccttt ccccctttgt taattcccgt atttgcggct
     5161 ttaagattag ccaaattcaa catcgataga aggcaggaag aagaattcag aggtttgcct
     5221 acgcctgcaa tggcaatttt tatcgcttcc ttagaagctc cttatggatt ttcgacttta
     5281 tttctcaact caatcctctc cgcctttctt ttccatccct tgaccttgtc gctgctggct
     5341 ttgttacttt gcttcatgat gatttcggaa atccccttgt tcagtcttaa gatgaaaagt
     5401 ttttctttta aggagtatcc ttatccgatg gttttccttt gcgtttcctt gatgcttgtt
     5461 cttatcttta agactgctgg attgggtttt tccattttga cctatgtttt cctttcactg
     5521 gtaaagctct tgtttcggaa gagggaatag aagattctat cgccgtaatt cgaaattctg
     5581 tcccagataa acccttctca cctgttcgtc attggcaagt tcttctgatg ttcccgattt
     5641 catgacactt ccttcgtaaa gcagataggc gcggtcggtt atgctgaggg tttcatgaac
     5701 attgtggtcg gtaattagta cgccaatatt tttatccttt aggtgagcca caatattttg
     5761 aaggtcttcg acagcgatag ggtcgatacc agcgaagggt tcgtctaaaa gaataaactt
     5821 ggggtcgact gccaaagcac gagcaatctc ggttcttcgg cgttcacctc ctgataattg
     5881 aatacccttg cttttgcgaa tatgattgag cccgaactcg gtaagcaatt cttccaagcg
     5941 ttttttttgt tcatttttgc taaaatgact gaattccaac acagccttta tattatcttc
     6001 tacactcaag ttacgaaata ccgatgcttc ctgagccaga tacccaactc caagctgagc
     6061 tcgtttatac atagggaatg atgtaatctc ctgatcatcg atgaatactt tgcctgcaaa
     6121 gggttttatt aaacccacaa tgatgtagaa agtcgtcgtt ttccctgccc cattgggccc
     6181 aagcaatccc actatctctt tctggttcac ttcaaccgac acatctttca caaccatacg
     6241 attgcgatac tttttgtata aaccctgagt gtgcagtttc ataaatgatt ctgagggtta
     6301 agttcttccc aaaattcgaa ggctcttcgt gtatgtggta taacgatggt gcctcctatt
     6361 aaattgacga tgccgaagag ttcgagtaat tcttcggttg taaccccgag ttgatggcat
     6421 tggatgagat ggtagcgaat gcaatcgtca catcgaagca ccatacttgt agctaagccc
     6481 agcatttctt tggtctttgc attcaatgcc ccatccttat aagccatggc atcgacgctg
     6541 taaattcgtt ttaaaactat attattgggt gcagccagaa tcttttcatt cattctctgg
     6601 cgatattcct tgaaatcatc cacgttatct ttcattttta gcaaataatc aatacattaa
     6661 accacccctt ctttcaaaat atcatgtaag tgaattacgc caagataaat accattttcg
     6721 gtaaccagca attgagtaat gttattggag cgcatcacat ccagggcatc agccaccaga
     6781 ctcgagggta ggattgtttt aggcgaagtg gtcataacat cagatgccac aatatttttc
     6841 agttctatat ttttttgaag cattcgccta aggtcgccat cggtaattat gcctatcaat
     6901 cgatcttggt caagaactgc ggtacagccg aggcgtttgg aagagatttc gaaaattact
     6961 tgagtaatgg gcgtatgagt ttcaacctga ggtttttcgt tccttagata gagatcagaa
     7021 accctcaggt ataatttttt ccctaatgca cctccaggat gtagtcgagc aaaatcctcg
     7081 atagtaaatc ctctggcagt cagcaatgct actgccaatg catcgcacat ggccagctga
     7141 gccgcagtac tggctgtagg aacgaggttg ttcggacagg cttcatgctc aacatgtaca
     7201 ttgagaacga tgtcggattg gagacctaaa taggattgag gatttcctac gattccgatc
     7261 aaagtatttc cattggcctt aattagtggc aataagactt ttatttcggg agtatcgcca
     7321 cttttcgaga ggcaaactac tatatcgtct gggcgaacca ttccaagatc accatgaatg
     7381 gcatcggcag catgaagaaa aaatgcaggg gtacccgtcg aattcaaggt ggctgtgaat
     7441 ttccgaccta tatctgagct tttccctatc ccggttatta taactctacc acttgcttcc
     7501 agaatcacct ctatagcatg aacaaattca tcatcaatgt gttgctttaa accctgaatg
     7561 gctagcgctt cttcatgcaa ggtctgctca gctattttcc tgatgttctc tttttccacc
     7621 ctttagcaat ttttaatgtt gcaaagataa gaatatacct tacttctccg ttctaatcga
     7681 gagatgatga tccaataaac cgcttaggaa atgaggatcc aacgcatatt atagaatatc
     7741 atacttacct caaagtcatt gcaaaccctt cactttcttc atcaccccct gaagttaagt
     7801 tgcagaaaaa attattttaa tagattgaat ttcaatgtat tgtaatacgt cgagtgaaat
     7861 aaacgtcgga agaaaacctt attgacctct tgtcagaatg aaaaatggaa aaatttttac
     7921 atgaaaattt aaaagtttca gatttttata ttatttttac aatacagaat gaagatgcat
     7981 ttttgtcgtt ggaaagaaat aaaatgtatc cccaaaaggg gaatggagcg ttggatatgt
     8041 tagaaatatt atatttgcat ttccttttta taaacataaa tatttgatta tcaatgttta
     8101 aattgaatat tcatcattat gagtccatta ctggtcggtt ggccagaatg gataaatata
     8161 aaaaattgtt aaaggagtag aatgaaagtc gagttaaggg aaaaagttgt agtaaaagat
     8221 ccactttaca gagccttgaa ggaagtattt ggctttgaga gttttaaggg ttcacaagaa
     8281 gtcatcatac gtaatttaat ggctggcaaa gatacttttg tgattatgcc gacgggcagc
     8341 gggaaatcat tatgttatca gcttcctgcg atcatatcgg aaggtacggc cattgttgta
     8401 tctcccttga tagctttgat gaagaatcag gttgatgcaa tacggaattt tgggatggag
     8461 attgggctgg cgcattattt aaacagtagc ctgtcgcgtc aggaactggc acaagtgaag
     8521 gacgatttgg cagcaggaag gaccaagctt ctgtatgttg ctcccgaatc gctcaccaag
     8581 gatgacaaca ttgaactttt taaatccatc aagttatcgt tttatgcgat tgatgaagcg
     8641 cactgcataa gtgaatgggg gcatgatttc aggccagaat acaggaggat aagaccgatg
     8701 attgaagcca ttgggcagca agtccccgta atggctttaa cggctactgc tacacccaag
     8761 gtgcagcagg atatacaaaa gaacctggcc atgatgaacg ccactctatt caaaacttct
     8821 ttcaaccggc ccaatctgta ttatgaagtt aggcccaaaa ccgagaatgt aatcaaggat
     8881 atcatcaaat atatcaagag ccaacctgga aagtcgggca tagtatattg cctgagtcgg
     8941 aagaaggtag aagaagtagc cgaaacgctg caagtgaacg ggatcagggc tattccttat
     9001 catgcagggc tcgatcctca aaccaggcgt gaaaatcaag atagttttct aatggaagac
     9061 gctgatgtgg tagtagctac cattgccttt ggaatgggga tcgacaaacc ggatatacgt
     9121 tacgttatcc atcacgacat tcccaagagc ctggagggtt actatcagga aacaggccgg
     9181 gccggacggg atggtggtga aggtaattgc ttagcctttt acagttatga cgacatcttg
     9241 aaactcgaaa agttcctgaa aaataaacat gtagccgagc aggaaatcac taaactcctg
     9301 cttagtgaaa ccgtggctta tgcagaaacc acgatctgcc gacgaaaagt acttcttcat
     9361 tactttggag aacaatacac acaagataac tgtggcaact gcgacaattg tctgcatcct
     9421 aaaacacgtt acgacggaaa agaagccgtt ctaaccgtgt tggatgctat aacacaaacc
     9481 aaacaacaat gcaaatcgaa acatctggtg catgtaatta taggaaaact tactgcaaat
     9541 gtcaaagcag ccaaacatca caaactcgag gttttcggta gaggtgaaga atatgatgaa
     9601 ccttactggc atagcgttgt aagacaaatg ctggtagaaa acctcatcga gaaagacata
     9661 gaaaattatg gcattcttag aattacaaat gaaggcaaaa agtttctcaa aaaaccaaaa
     9721 acgattatgc ttactcagga ccgcgaatac gacaacaacg aagacgacga actaatcgat
     9781 caaaatacag gcgctaaacc tggcacagca gatgcaacac tctttgccct cttaaaagat
     9841 ttgcgcaaag aaatatcgag aaaagaaaac ctgcctcctt tcgtcatctt tcaagaccct
     9901 agcctggaag aaatggctat tcaatatcct attactctgg atgaactgaa acaaattacc
     9961 ggagtaggaa ccggcaaagc ctcgaaattt ggaaaaccct tcatcgatct gattaaaaac
    10021 tatgtcgaag aaaacgaaat tatccgccct aacgatatgg ttatcaaatc agtaatcaat
    10081 aaatcgggtt tgaaaattta tatcattcag agcatcgacc gtaaagtccc cttagaagac
    10141 attgctttag ccaaaaatct ttctttcgac gagctgctta ccgaaatcga acacattgtt
    10201 gcttcgggaa ccaagataga cataagttat tatatcaacg aatatatcga tgaataccat
    10261 caagaagagg tttttgaata tttccgcaca gccgaaaccg attctatcga aaaggcacgc
    10321 gaagaacttg gagaagaaga attttcggaa gaagaaattc gcctaatgcg tatcaaattt
    10381 atgtcggaaa tggggaatta aacgatattc atttcgaaaa tgcaagggtt gctttatgat
    10441 taactttggc caatctcata gcaaacccag ctgtgtttat ttgttcgatc aatcactctt
    10501 cataaaattt ctgtccccta tatgaacttc aacatcaata atacgagcat tctttaactt
    10561 tgctagtcaa acctttcttt attcttataa ttatgaagaa caaattcaat cttagcctta
    10621 ttctcagcct tttatcgcta tttggcgtaa ttgtcctgtt tattctctat tttacacatc
    10681 ggccattggg tagagaaggc ataccaaaag ctgccgaaac gggaggccat cgcattgctt
    10741 ttttccgaac cgattccatt ctccatcatt atgatttcgt aaaggaaaga gctgaagaac
    10801 ttgcgcaaaa atcacgaaaa tatggggaag accttcatcg aagacaatcg gaattcgaaa
    10861 atgaagccgc ttattttcaa gaaagtgtca gaaaaaatgc cctttccgaa aaaagtgctc
    10921 aggaaattta tcaagaacta atgcaaaagc agcagagcat aatcgatctg aaagatcgct
    10981 atgatatgga gctggctggc gaagaagcac aaatcaacct catcttgttc gactcggtaa
    11041 ccaatttgct gaaacgtttc aacaaaagct atggttatga ttatatcctt ggctacaata
    11101 aaaatggaaa tgttttctac accaacgaca cattcgatat tactcagatg gttattaaag
    11161 atttgaacag agaatacagg gccgttaagg gtaaaacatc tgggtctaaa taagaataaa
    11221 gcattttatg aaaaaagata aatgcttatt tctaatgata acggttttat tgttcattgg
    11281 ctgcattcag cccaaagaga aaaaacctgg tgctctcatc acgccccgaa agatggctga
    11341 gattctcacc gatttgcaga ttgcagatgc ctggattgcc ataaaacaaa acgagggtca
    11401 ggatattaaa ttgctttcta ctcaaatcga agattcgctt tttaagaaac accatattac
    11461 ccgggctcag ttcgatacga gtatggtttg gtattcggca catgcagaag agctcgacaa
    11521 aatttacgac caggtaatcc agcagcttga attgttgaaa gagcagaaca agacatcaaa
    11581 aaaacaaaat tagagccgcc gaaaagttgc tttgaagctt agttcattgg ctttgtaaga
    11641 gctccattcg atgacgttgt ttttcattat cccaaatcct gaaactgtcc agcttccccc
    11701 tgccacttgc tgttgaggaa cagtaatagt ggatgacgta acaatagcaa atggagcaac
    11761 atcgtcccct aactggaaat aattatacaa cttaacttgt gccgaattgt cagggtcaat
    11821 cattattctt acgatatatg cgttttgggt ggccttgttg ggagattctg tgcatatcca
    11881 ttgacccagc caaggcgtgc gctcgtcgtc accatcgggt gagatttcga tatcctcgca
    11941 agcttgaaga acaaacatca ggctaaaaat cactatccaa acaaattcta tattttttaa
    12001 tctaaattgc cctaacagag aatggctttt catggttatt tactttttta tttttcgctg
    12061 gtaaaacaaa gtaacataaa aactcagcaa gatggtcatc acagcttgaa tccacaaagg
    12121 ggtggacagg agttctccct ctatccatcc aaattgatga cttgcaaatt tcatactata
    12181 gtaaatagat ttgctgatga ttatgctcac caatactacc cataacaatt tccatttttt
    12241 ctcgaggtaa tatagaagtg aaacattgat taacaattcg gtagacagaa gcaaggtttt
    12301 tacgatatga ggatgtccac cagccaaatg cgaaagaagt ggcaagccta aggctaaaat
    12361 gaaggtattg ggtttattgg taaaaacaag agccgtgaga acccatatac gcatagggtc
    12421 gatcaagtaa aaggggatgc ctgttatttt gaccatacgg ggcatgaaac agataaaggc
    12481 tatggccgca aaatctacca ataatattgg aaagactgaa gacgttgaat tgtttagatg
    12541 ggttgttgct gcaaaatttc gatccctatc cataaatgtt tttatatgtt ggattcgata
    12601 agtgctttaa caaggtggct tgcacctatt ctgaatagct ggggttttag ccattcaggg
    12661 ccgagaacct gttctactaa ggtataatat aaaattgcct gactgggtgt ttgaattccc
    12721 cccgctggtt taaacccaat ttttttacct gtagaatgat agaattcgag gatagtatcg
    12781 cacattacta aggctgcttc aggggttgct gcaggcgtga tttttcctgt gcttgtcttc
    12841 aaaaatgcgg ctcctgcttc catagctagc aagccagctt ttcgtatgtt cctcgtgctt
    12901 tcgagttcac ccgtctcgag gattactttt acggggattt cttttgctac atcgactatg
    12961 gcttttattt cgtcgaatac ttccttggtt ttcccctcta aaaattttcc gcgattgatg
    13021 acgatgtcga tttcattggc cccttcctgg agagtccatt ccacttcggc caagcggatc
    13081 gaaaaggggc tctgccctga aggaaatgcg cctgctacgg caactgtgcg aatgccagaa
    13141 tcttttaata aggattttac aaatcgggta aatacaggat aaacacaaac agcagctact
    13201 ttattagaca ataaaagagc taaaaggcaa aggttaccta tactttgcct attgtccttt
    13261 ccacctaaag aggttaggtc gataaacgaa taaagttgct ttaagaggtg tgcatctttt
    13321 tcttttgtac tcgctattgt ttttatctct tcgattcttt ctttgttttc ttcttcagaa
    13381 aaggggtata tattcaatgg tttcattttt cgaggttttt agtaatttca atttccaact
    13441 ttaagaaatg tcatcgattg gaatcagatt atctaaggat tctcatgatg tcgttaccta
    13501 tagcaaatac cataatcccc aaaagaataa tcataccagc gatttgagca tattcgagga
    13561 ctttttcgct gggtttgcgg cgagttacta tttcgatgag caagaaaagt acatgtccgc
    13621 catcgagagc ggggataggg aggatattca tgaaggcgag gataagcgaa agcaggcctg
    13681 taatgtacca aaattttgcc cagatccatt gagagccata gatttgggct atgccaatag
    13741 gcccttgaag gctgtcgctg gctttttcct caccgctgat gattttccca aggcccttga
    13801 tattggccga aagcatatcc atagcgtcct tatagccata agaaagggct ttcccaaaac
    13861 tataaggtgc ctgtgcataa ggtatggaag ccaaaatacc aataataccc gatgaatcga
    13921 ctgtgagttg catgttcaga gtgtcgtttc ctcttagtgt ttgaattgct accagacgac
    13981 ctgcagaatt agtgatcagt tccttgaatt gcccgtaaga ttttatttct atagagtcga
    14041 ttttgagaat cttgtcagac tttgttaaac ctgcatttgc cgcaggcaat ccggggaaaa
    14101 cgctatctat acgaaacgga aagttgagag gttcgatgaa tcttacgcca cggctactgg
    14161 cttcacgacg gtagaaatct tccgggatct cgatggtaat aattttaccg tttcgttcta
    14221 cttcgaaagt gctgccgaag agcaagtcga ctgaatgtac atctttgaag cgctcaacgg
    14281 gtttgccatt gatgcgtagg attttgtcgc cggcctcgaa accaatttct ttgccttttg
    14341 ccgaggcata tatcccatct ttatttattt cattcaaagg gagatatgat ttttcataat
    14401 gtaaaagtgt gaagccaaat atgaggatac ccaagataag attgacgaaa acacctgcga
    14461 gcattacaat gaggcgctgc caggcaggtt ttgaacgaaa ttcccagggt tgtggaggag
    14521 atttcagttg ttccttatcc attgattcgt cgatcatgcc ggcaatcttt acatagccac
    14581 ccaaaggaag ccatcccaca ccatactcgg tttccccttt cttgaaactg aataatttga
    14641 agtttccggc gtcaaaaaac aaataaaatt tttctactct gattttgaaa gcgcgggccg
    14701 caagaaaatg acctaactca tgaaggatca ctaaaatact gagacctaaa atgagctgac
    14761 cggtcatgat aagaatattc ataaaatttt gtggtattga tttttaattt attacattca
    14821 attaacaaac atcgagccta atggtttata aaaatccttg agctacttct ctggttattc
    14881 tgtccgtttc gatgagttgc gaaagatcgg gcgacgaaat aaaatctatc atgttcatac
    14941 atttttcgat gattactggc attcgcagaa aagggatgcg atgttgaagg aaagcttcaa
    15001 cggctatttc gttggcagca ttgagaatgc aaggcatatt tcctccctga tgcaaggctt
    15061 cgaaggcgag tattagattt ctgaaaacat gggtattggg tgcttcgaaa gtgagtggag
    15121 aatttctgat aaagtcgaaa cgctgaaatg acgagggtag ccgttcaggg taggatagag
    15181 cgtactgaat gggcaaacgc atatcgggta tgctcatctg agcctttatt gaaccatcca
    15241 caaattgtac catagaatgg attacgcttt ggggatgaat gattacttcg atttggtttg
    15301 gattcagatg aaacagccat ctggcttcga ttacttcaag gcctttattc atcaatgtag
    15361 ccgaatcgac cgaaatttta ggacccatgt tccatgtggg atggtgtaag gcatcttcgg
    15421 gtttcacatt ttgcaacctt tcgagactca tccctctgaa tgggccacca gaacccgtaa
    15481 ggatgatttt ttcaacctta ttcggttttt cgccctcgag acattgaaaa atagccgaat
    15541 gttcagaatc gatagggaga agatctacct ggagagattt taggcggttc ataacccatt
    15601 cgcctgccac cacaagcgtt tccttattgg ccaaggcaat atgttttttt gcttctatgg
    15661 ctgctatcac cgaccgaagg cctgcaaaac cgacgatagc tgatactacc atgtcaattt
    15721 cgggcattgc aacaacttct gccagcgcat tttcaccggc agccacatcg atgtgatcct
    15781 gttcgaaggc ctgtcgcagc acttcaaaat cacttgattt ggcaacagca accactttgg
    15841 gtctgaattc ctttgcctgc gataccagag ccttcgaaaa tccttgagca gccaatattt
    15901 ccaccacaaa actctgagga tgttcccgaa ctacatcgag gcattgacga ccaatgcttc
    15961 ctgtacaacc aagtatagcc aagtgtttgg gattcattag aatgctatgt aatttttagg
    16021 gtcaattgcc gtaccattat accagagctc gaaatgaaga tgtgtccccg tgctgagtac
    16081 acctgtagaa cccgcaattg caatgggctc acccgccttg acaatatcac cttgcttgcg
    16141 cagtaaagat gaattgtgca tataaataga aactacattc gatatatgtt gtatagtaat
    16201 ggtatatcca gtctctatat tccatcctgc ctgaatgact gtgccatcga gtatagcttt
    16261 tactgtttca ccctcggcag ttgcaatatc tacaccaaaa tgcccatgcc ccggatcaaa
    16321 atgtgaagtt agataaccat ttacaggtct gaaaaaattg aacgacgaaa tatatgaact
    16381 ctttattata gatgttgaat atccaggctt caacccatac ttgtcctgat tttccacctc
    16441 cttgcgcaat attgaatcct caggtattct ggtatcgtct atcttcgaat aatcgtgctt
    16501 ttgaatagtg ctgtcggcag ccttacgtaa aggaacatta cccgtaagca ctctttgaat
    16561 attttccagg taagttgctc tttgatcgag taaaagttcg agtgaatcag cccgtaaagt
    16621 aagttcgtaa acctgacgac taagatcggg attgctgtat ccaggaatat attctttgag
    16681 agaagtaaag gcaattagat aggtggtgag aaaaataaga ccaatgataa gaatacccaa
    16741 agtaataaac acattgaggc gcgatagctt gaagctgaat ttctcctcga aggtttcggc
    16801 atcctgaata actaatctat actttgattt aatattccgg aaaagaacag tccttatgtt
    16861 tttaacattg atatgcataa ttcaatctgc taccaggaat attccctgaa tcctcaaagc
    16921 catcagtttt aaagcaatga ataacttaat tctatctgta aatacttgta tgagaatgca
    16981 agtgatttaa ttggccgcaa atttaatgat aaacactcta cgttccattt tgtgatttat
    17041 cagcatgaat tcccccaatc gacacggaag gcaaactgga attctgaagc attgaatttc
    17101 ttatcgatga tgttattatt ggtgataacc tacttctgtt atgtacaatg ttttttatca
    17161 atcagataac attgtaattc gtccagaaaa ctagcaagct aattctcatt gccgataatt
    17221 tagccttcaa cgatggttta tcgatctttc ggtagatatc acggatggtt tttactgttt
    17281 taacagtcga ctttgagggt tttccctttt ggttagtaaa gagaggtagc ggttttacaa
    17341 taaagaatca ccatttatcg gccaaggagg taaaattgag agttaaagtg gcaatgtttc
    17401 accaaaccaa ggaaatgaaa taattttgca tcttcgaagt tattcaatat acgttaattt
    17461 ccagaaaaac cgattatttt gaaaagtaaa aacttcatac ccaaaggtat aatcattttt
    17521 ctttggattg ttacaattat tattcctacg ggctgttcga ataagaaaaa cacgtttagc
    17581 cgcagagttt atcacaatct tacggctcat tacaatacgt ggtggaatgg gaatgagagt
    17641 ttgaaagaag ccatcaagga tctggaaaag aatgcaaagg ataattatac cgaggtgctt
    17701 cccgtttata agttgggatc aaagaaggag gcaactgcta ttaattcgaa agccgatcga
    17761 gccatcgaaa aggcttcaaa ggttatacag aataacagca tgtatttcaa taaaaaagaa
    17821 tataaccgat ggatagacga cagttatatg atgataggga aggcatattt ttacaagcag
    17881 gattatgcct cagctcgaag gactttcgaa tttatcctca cccgttataa agattctcct
    17941 ctgattccag aaatttatct ttggttagga ttaaccaaca atcaaatgga gcaattttcg
    18001 cgctctgaag ctgaattgga gaaatttcgt atgctttcgg ctcgaaaaac catgccacgc
    18061 agtatgatgt tattctacaa caaagtttta gccgatttct acctaaaaca gaatagaaca
    18121 gaggaggcca tcaaaaccct tcatattgcc acggatttaa gccgaaaaaa atctgacaag
    18181 acacgtcttc atttcatttt gggacagttg tatatgggaa agaacgatct tatcaaggcc
    18241 tttcaaatgt ttcaggaagt cattaaacgg actcctcctt acgacatgca gtttgcggca
    18301 cagctcaatc ttgcccgctg tttcgatgta gcttcgggtg aagccgaaaa gctcgaaaag
    18361 atgcttcaaa acatgttgaa ggaagagaaa aataaatttt atcaagacca aatttattat
    18421 gctctggctg agatagcctt aaaaaaggga gaccgtactg cagcaatcga aaatcttcgc
    18481 ctttcggtag ctaagagtaa agataatgat tttcaaaggg ttcaatcctc attgacttta
    18541 gccgacctgt atttcgagaa gcctgattat ttttgctcac aagcttatta tgacactgct
    18601 ttgcaggcct tgcccaaaga tcatcccact tatgaggctt tgcaaaagaa atctcaggtt
    18661 cttaccgaat tagtaagcaa ccttacagta gtacaaaccg aagatagcct ccagcgtttg
    18721 gctcaaatac ccgaaaatga acgtttgaaa atcatcgatg gcattattgc aaagtatgcc
    18781 gaagaagaaa agaaaaaaca agaagaagaa atgcttcgcc agagcgacct taccatgctg
    18841 gggcaaggca atattcagcg ccagatggac ccttcgggcg cctggtattt ttataaccct
    18901 tcggctataa gcctcggttt tactgaattc aaaaggaaat ggggcaaccg aaagctcgaa
    18961 gacctttgga gactcagcaa caaacaaata accactgcgt gggaagaaga acaaactatg
    19021 gccttttccg attcagtggc taatgacagc ctgttggtaa agatttctac cgatcccttg
    19081 aagcgccaaa cttatttgca aaaccttcca ttaacacccg aaaaacttcg tcaaagcaac
    19141 cagaatatta tcgaagcgct tttcaacgca gccttcattt atcgcgaagg attgctcgat
    19201 atggtaaatg ccgaaaaaac cttcgaggaa ttggtagaac gtttccctga taccagttta
    19261 aacaaacatt atttgcttgg ttgttaccaa ttggtagtga ttaacgaaaa gcttgagaat
    19321 cagtcaaaag ccgatcatta ccgtaatatt ctgattacga actatcc
//
LOCUS       DAOORU010000023        19339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_3443_length_19339_cov_7.393020, whole genome shotgun sequence.
ACCESSION   DAOORU010000023 DAOORU010000000
VERSION     DAOORU010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19339
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3443_length_19339_cov_7.393020"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            466..2391
                     /locus_tag="PLF20_02885"
     CDS             466..2391
                     /locus_tag="PLF20_02885"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HOX79986.1"
                     /translation="MIKKLFNKYYSHRFISRWITFTFDLSVAVAAYFMALFVRYSISP
                     HAYPWFIFEIVLILLSYSFAFLTTRSYVGIIRHTGIKDITRILQATSFAFILLITGEY
                     IVQRFQLIHNFTIAAGVIIVQFALCNLFLIGSRIIIKVIFMEITEQEHSNRTFVLIYG
                     AGSAGMLTRSALQQDSNHNYKIIGFIDDNESKVNKSIDGTKVYSPTQVFNEKFINRHH
                     ITQMILAILNIDNEKRRAIIEKALEVNLQVKNIPPAENWINGQLSVNQIREIKIEDLL
                     GRPPIKLDNSHVKEDLLSKVVLITGAAGSIGSEIVRQVLEYQPTRVILVDQAESALFD
                     LQSELRYSKKYSDILQQVVFIVANIKEKTRMKQIFETYKPQIIYHAAAYKHVPLMEDN
                     PYEAVMVNVFGTKNIADLAVEYKVDKFVMVSTDKAVNPTNVMGASKRIAEIYTQALSN
                     GTTRFITTRFGNVLGSNGSVVLLFKKQIEKGGPVTVTHKDIRRYFMTIPEACSLVLEA
                     GAMGQGGEIFVFDMGQPVKIYDLAIKMIQLSGLRLGKDIQIEEVGLRPGEKLFEELLN
                     DGENVIPTSHPKILGAKVKMYHPEEIEKMMFELGQTVIENDSYKLVKKMKEYVPEYIS
                     NNSIYSILDAQPQPFVQ"
     assembly_gap    2676..2775
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2969..4591
                     /locus_tag="PLF20_02890"
     CDS             2969..4591
                     /locus_tag="PLF20_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79987.1"
                     /translation="MVICPKNFKTFLLLLLSISTQILSSQPGIERSINPDYLLPDRYL
                     YHPDANFHTSVRPYLRNEVNEVINYDSILLEQPLKSPWKGKTGEWIWKKVFHEDLIQI
                     TGKDFHFAVNPLVNFEIGRDFKNDDNAWVNTRGFMAEGNIGSKFFFATRFYENQATFI
                     SYIDKDIQSKLVIPQQGAYKRFKTDGYDFSRSEGYISFSPNKYFNFRLGHGRNFFGDG
                     YRSLLLGDAHFPYPYFLISTQIWRIKYINLYAEFMDRKAPHSESLGYKKKYGTFHYLT
                     TNITNRLKVGLFEAIIWQAEDSTGYRGFDFNYLNPIIFYRPVEFSLGSPDNALMGLNI
                     SYTIGKKTAFYGQFLLDEFQLKEITSGKGWWANKQAFQLGMKWFDIFHVAGLDFQSEV
                     NVIRPYTYSHSDPITNYGHYSEPLAHPLGANLVESQNFVRYHHGRWHLTYQLQRVQYG
                     ADTAGLNFGKDIFISYSNHPFDYGNKIGQGLKTILWRHDFKASWVINPAYNLNLSTGI
                     VYRQLKTDLTNDQTLFIYIGLSTSLEKLYYDF"
     gene            5079..5435
                     /locus_tag="PLF20_02895"
     CDS             5079..5435
                     /locus_tag="PLF20_02895"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOX79988.1"
                     /translation="MMRKLIFFIYLGLITTLSLIPSSDLPSVKLFSGADKIIHFLMYT
                     GFTFLLMWAWPNFFRGSLLLLPLFAVMFYGLGLEILQDLGHEGRAFEIYDILANTLGY
                     FPGLALFYIVRKYENH"
     gene            complement(5432..8764)
                     /locus_tag="PLF20_02900"
     CDS             complement(5432..8764)
                     /locus_tag="PLF20_02900"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOX79989.1"
                     /translation="MNTPRFEIYRASAGSGKTFLLSIHFLSLVLEDPEVFRRILGLTF
                     TNKAAKELKERILYFLGLLAKLPNCNDGKLKVSILENLKNEGWNSSDEQTQQKARKAL
                     ENILHYYGYFSISTIDAFMYRVVNSFTFDMKIAHDFEVELNEDVLIDEAIQRLLDRVG
                     REKVITELLQNILLEMVDDERGWNIEREIREMAEIIFLEKSNEPLSELGTYTPEQIQE
                     ISSNVREYSKCIKKEAQRIAKDVLQKIASTGLSESDFKGGSKGSCLVWVKKFTEEDIK
                     NITTFKSVNEALENDVWFTGKNDTKQSNFRPIKAEIKKNLEELIRLKREYLLTSIVYD
                     SLFRLPLYSNLYRTLEEIKLDNNVLHISDFNRLISKVIRSEPVPYIYVRLGMRFKHFL
                     LDEFQDTSVQQWHNLVPLLHNALSGGEDGYARIVIVGDGKQSIYRWRNGELEIFEKLP
                     QIFRKPEQQNFDEAEHLFASFSSQKPKVLKENFRSLKSIVDFNHKFFSHIKESLGEEA
                     RRIYRDTKQETDKSRQGGLVQWEWVTKNDPKDDSDNHLKRISQIINELLNHSDKKYSL
                     NEMAILVRKNDEAQRIADFLIQKNIPVVSPDSLMLNQSESVRRVISWLKLIAQPDDRL
                     ALATAIELNQGMKDVPEEKELLHLTQNNREKNLLQKSGEEILKNVFGSYSSTLFSNDL
                     YTLTQKIMKDCQLNDPPDIFCQAFLEKIKGQNRYTPFSLEEFLDEWDQNQDTWAVSFP
                     EDMDAIRIMTIHKAKGLEFPVVIRPYLFSRPNYNSTEWVATSEILDGIDRDFWSNFNI
                     PWLLLDSSSKFKDSGIQGLEKFYNDLIEKQLIEDLNLMYVAFTRAKERLYIFAYPPSS
                     SDSDSNLLPKESKKVLEEHFCFKDGQIFIYSEDATKKQQDEVTGKLGEEETKRLENFK
                     TIETHCEGHEEKDKTMDEDIERLGDKEIEENVISTRMHIRPLAPQKWEASSPDTEREL
                     GKMIHLLLDHSQESEVLKNIPEFHQMTKKMGETFGSALIENLRILKNDPLYSAVFERQ
                     GEKLTEREILLPDGKWLRPDLIILGDGFTVVADYKTGQPHPQYEKQMHKYIETLLAAG
                     YPSVEGYLVYLGNPPHIERAGI"
     gene            8856..10049
                     /locus_tag="PLF20_02905"
     CDS             8856..10049
                     /locus_tag="PLF20_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOX79990.1"
                     /translation="MSKFATIMVALEEAIEKVMNEARVLPMEEISLTEAQGLVLAEDV
                     FSDLDMPPFRKAAVDGFACRREDLDKPLKVIETIAAGEVPQKSIGVQECTRIMTGAMV
                     PEGADCVVMIEHTIGEDEGTIRFIGDKTSDNIAQKGEDIRKGEKVLSAGTVLEAQHIA
                     VLASVGRFTPKVYRRPLVGIIATGDELVEPWEQPKAGQIRNSNAWQTITQVKKTGAEY
                     IYGGIVKDNPQALSEKINLFRQKVDVLLFSGGISMGEYDYVPQVMKDAGLRLIFKDID
                     IQPGRPTLFGILDHIAVFGLPGNPVSSFVQFELMVHPFIYTSMGKTFHAHIRHLPIGE
                     DFYRTKANRRSLIPVRIENDWIFPVKYHGSAHINAYTFASGWIDIPKGKKELKKGEMI
                     DVRLL"
     gene            10033..10833
                     /locus_tag="PLF20_02910"
     CDS             10033..10833
                     /locus_tag="PLF20_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX79991.1"
                     /translation="MLDSYRREINYLRISVTDRCNLRCRYCMPSEGFRWIPHENILRF
                     EEIIEIVREAVKLGIHKIRVTGGEPLLRKGIVNLIEMLASIEGVQDLSMTTNGTHLEK
                     FARELKQAGLQRINISLDTVNAERYAYLTGGGNIERVLRGIDAALEAELTPIKLNCVI
                     QHNSNEIDARTVTKFANQKGLEIRYIHQMNLAEGIFSAVEGGDGGHCEQCNRLRLTAD
                     GFIKPCLFSDLRYEVRQLGARNALLETIRNKPKEGTTNRINYFYNIGG"
     gene            10894..11463
                     /locus_tag="PLF20_02915"
     CDS             10894..11463
                     /locus_tag="PLF20_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79992.1"
                     /translation="MQDLEPYKSWHYLYNAARDIRSPFFGRSYHKTLCTHTIYNYYIH
                     PQWDEIGSPTLYIKILFADYIRRFCVIEMLGEWNDCINNDIMYLKRNVVDALTSEGIR
                     YFILIGENVLNYHSDTDDYYQEWFDDIEDGYIIGLNFREHVVQEFIHARLDNYMLFGG
                     RFSEIPWRKFSPQHLFDLTKKMITRRLSE"
     gene            11654..11929
                     /locus_tag="PLF20_02920"
     CDS             11654..11929
                     /locus_tag="PLF20_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOX79993.1"
                     /translation="MVKHYDITLLYESQIEGFRFSMMEKALRLNITGYVQRQLNNSVF
                     IEAEGEKTNLEKFLEWCKKGPLGVNILRVEVTEGEVKGYSTFDIQHR"
     gene            complement(11926..12408)
                     /locus_tag="PLF20_02925"
     CDS             complement(11926..12408)
                     /locus_tag="PLF20_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOX79994.1"
                     /translation="MRKNFSARSIQQFNVRVYGIYMNATSVLVTDEFCLGMRMTKFPG
                     GGLEFGEGTLDGLRREIKEELHQEPVILKHFYTTDFFQRTLLVEPPSQLLSIYYLIDL
                     PFPRKISLVDKPFDFSVEQEGAQTFRMLNLHESDEVMTFPIDRHVWKLLQKTFLQELP
                     "
     gene            complement(12405..13073)
                     /gene="mnmD"
                     /locus_tag="PLF20_02930"
     CDS             complement(12405..13073)
                     /gene="mnmD"
                     /locus_tag="PLF20_02930"
                     /inference="COORDINATES: protein motif:HMM:NF033855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD"
                     /protein_id="HOX79995.1"
                     /translation="MTEVVVTEDGSPTLWIEEYSEPYHSLHGAVQEAMHVFIHTGLGS
                     IASARQTIHILEVGFGTGLNALLTWDYAQLHKLPVSYTGTDPFLPPPSLLKGLNYPVY
                     CRSPYAEQAYAKILSSFDGSRVIKIDELFSLQVIQEDFRKISYPDESYDCVFFDAFRP
                     EIVPELWEKQAMQFLYNALSRGGILVTYSTKGQVRRNLSACGFLVEKVPGPPGKREIT
                     LARK"
     gene            13182..13880
                     /locus_tag="PLF20_02935"
     CDS             13182..13880
                     /locus_tag="PLF20_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOX79996.1"
                     /translation="MIADKILFLDIETVPRWPDYDAMPEHFRVLWNHKANHIKNLPDD
                     TARSLYPRAGIYAEFGKIICISTGFFHQDCFRIKSFYGDDEKIMLEDFKTMLERMNST
                     QPYLLAAHNGKEFDFPYISRRMLVNGIMLPDVFDNSGKKPWETSFIDTMEMWKFGDYK
                     HYTSLALLAALFDIPSPKEDIDGSQVAIVYYESNDLKRIVRYCQKDVITVARLYCKFK
                     GLPAPADGQIVIIE"
     gene            13898..15334
                     /locus_tag="PLF20_02940"
     CDS             13898..15334
                     /locus_tag="PLF20_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79997.1"
                     /translation="MAKTELSKSTFIRALQCLKSVYLNKNRPFLRDHLEARQLNKFER
                     GHEIGSLAWKLFPGGIDCSPKHPAAYAKAAAKTTELIRRGAKILYEAVFEYDGIRVLP
                     DILVLNDSQIRMYEVKGSLNISQTYILDAALQYYVISGAGYTPSQTFILHVNKNYIYE
                     GGEIHPEDFFVFQDVTAEVIQQQNFIELKIPEAKAAVNADKSPEIPIGPHCYLPYPCD
                     FMGLCWKSIPADSPLYLNSIDIHSRFRLVSEGYSSISRIPLDSLDNELLKTEVEARIN
                     GKPIYKASELQKLLQPQKHQVYLKILFIENAVPRLAGTRPFEPLPIAWIAVKENGSLI
                     MGDSENQYQVFSEIINLLRNFLTEDDQLVFWGKPMELLIHQVEFDLPFLNLESVFQSE
                     NLYLPFYLEEKTPENIASQLGIINDEITTMNDDEAIRAWSGESSKGKFNSREIENHLK
                     MQLFLLRELYRYAAGFIETDHSIEAKKY"
     gene            15394..15669
                     /locus_tag="PLF20_02945"
     CDS             15394..15669
                     /locus_tag="PLF20_02945"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOX79998.1"
                     /translation="MTALQITISGRVQGVGFRYYIFRAAQELRIKGIIKNLPDGKVYI
                     EAEGDPQNIEIFLEKCREGPPLAYVTHIHITECLPSGYNDFRVAYGY"
     gene            15825..16502
                     /locus_tag="PLF20_02950"
     CDS             15825..16502
                     /locus_tag="PLF20_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX79999.1"
                     /translation="MKKEWKNKWDSIVKKVFSVESLPVQPLWLNFQRKQDEEEFTNQY
                     YKNILTRVRVWMLISTSGILLLQFIDYLLTGKFMNDAFAIRFEIFLPFSLLFILITFT
                     NLYIDFFQYLNLLWIFMTSLGGIITAILCPEASLPFILASMALFFIASFVLFGLKPYF
                     ALIGNTILAIGLLWILINQKILHPSYTWPIIILLFIFLIVGYYAGWKIELLERKLYWS
                     VKKQKSF"
     gene            16857..18212
                     /gene="purB"
                     /locus_tag="PLF20_02955"
     CDS             16857..18212
                     /gene="purB"
                     /locus_tag="PLF20_02955"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOX80000.1"
                     /translation="MFSNPLENISPIDGRYAGITWPLSNYFSELSLIKYRTRIEIEYF
                     IALCRLPLSPLKNFPRAGFQSLRKIYQNFNQKDAERVKEIEKTTNHDIKALEYFLRET
                     FDRLGFAEYKEFIHFGLTSQDINNTALPLALKEAISNVIRQPLIDILSQLHTAARSWK
                     NIPMLSHTHGQPASPTTLGKEIFVFVERLEKQITHLDYQSISAKFGGANGNLNAHKVA
                     YPEIDWITFANSFVNDVLGLERQQTTTQIEHYDDLASLFDNFARINTILIDLCRDLWL
                     YISMGYFLQKINEQEVGSSTMPHKINPIDFENAEGNLGLANSLFEHLARKLPISRLQR
                     DLTDSTVTRNLGVPLAHSLIAYHSILKGFDKIYPNETKLKEDLDTNWAVITEAIQTIL
                     RREGYPDAYETLKKLSRTGKPIDEQTIKEFIDSLEIAEPVKVELRQITPHNYIGIISF
                     E"
     gene            18209..19081
                     /locus_tag="PLF20_02960"
     CDS             18209..19081
                     /locus_tag="PLF20_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80001.1"
                     /translation="MKVSGLTIVRNAVQFDYPVVEAILSILPLCDEFIVGVGNSQDNT
                     LELVQQIASPKIRIFQSIWDENLRQGGRVLAVETDKAFQQISPDTDWCFYIQADEILH
                     ENDLDKVYENMRKNLSDRNVEGLLFRYLHFYGSYDYIGDSRKWYRHEVRIVRNSPKIH
                     SFRDAQGFRWDNRPIKVKDSGGTIFHYGWVRPPEVQAKRQKAFHRFWHDDQWIKTHLT
                     SNDFFDYSQIDSLSLFRGIHPRVMQDRIRSMNWKFDFDPTQKNFGFKAKILYIIEKIF
                     GWRPFEYRNYRLLK"
BASE COUNT         5942 a         3931 c         3604 g         5762 t
ORIGIN      
        1 cagttatctg ctgtcagtga tcagttgaca gttatcagtt aacagttatc agttaacagt
       61 tatcagttaa cagttatcag ttatttgttt taagttgtca acattaagtt ataagttatc
      121 agttgatatt ctgcgtgaat cagcgagaaa aaatacatct cccacagatt atcgcagaat
      181 aaaactcgca gaagaacgca gaaatgcatc tgcggaaatc agcgagaaag aaatagttag
      241 tgcagatcgt cttcgcagat attagtttaa ccccctatcg tgagcaaata caaattttta
      301 cgaaagtttt tctcattcag aaacattttt accaaaagca acgtatattt gttgaatgat
      361 taccgtaata acatatcaaa ggtgttttac gataagagag tctcatgtaa agaacaggtt
      421 tttggtatat tacagctaat gattttttat taaaaaacaa cagcaatgat taagaaatta
      481 ttcaacaagt attactctca tcggttcatt tctcgatgga ttacttttac ttttgactta
      541 agcgtagcgg tagcagctta ttttatggct ctattcgttc gctactctat ttcacctcat
      601 gcttacccat ggttcatatt tgaaatcgtt ctcatcttat tatcttattc atttgccttt
      661 ttaacaaccc gttcttatgt aggcatcatc cgccatacag gcataaaaga cattacacga
      721 attctgcaag ccacctcctt tgcttttatc cttcttataa caggtgaata cattgttcaa
      781 cgatttcaat tgattcacaa ttttacaatt gcagctggag taataattgt tcaatttgcc
      841 ttatgtaatc tttttctcat tggaagcagg attattatca aggttatttt tatggaaata
      901 accgaacaag agcatagtaa ccgtactttt gttcttatct atggagctgg ttcggcagga
      961 atgcttacac gtagtgccct tcaacaagat tcgaaccata attacaaaat aataggattt
     1021 attgatgata atgaaagtaa agtcaataaa agcattgatg gaacgaaagt ttattctcca
     1081 acacaagtct tcaatgaaaa atttatcaac aggcatcata ttactcagat gattcttgct
     1141 attctcaata tagataacga aaaaagaaga gcaatcatcg aaaaagcctt agaagttaat
     1201 ttacaggtaa agaatatacc tcctgctgaa aattggatta atggtcaatt gagtgtaaat
     1261 caaattcgtg aaataaaaat cgaggattta ttaggacgac ctcctataaa actcgacaat
     1321 agtcatgtaa aagaagatct tttaagtaaa gtggttctta tcactggtgc agcgggttct
     1381 ataggcagcg agatagtacg tcaggtatta gaatatcaac caacaagagt aattttggtg
     1441 gaccaagccg aatcagcctt gttcgatctt caatctgaac taaggtattc aaaaaaatac
     1501 agcgatattt tgcaacaagt ggtatttatt gtggccaata tcaaggaaaa aacaagaatg
     1561 aaacaaattt tcgaaactta taaaccacag ataatttatc atgctgcggc atacaaacat
     1621 gttccactta tggaagacaa tccttacgag gccgtgatgg taaacgtatt tggtacgaaa
     1681 aacatagccg atttagccgt ggaatataaa gtcgataaat ttgtgatggt ttcgacagat
     1741 aaggcggtta atcccaccaa tgtgatgggg gcaagcaaaa gaatagcaga aatttatacc
     1801 caggcgctta gcaatgggac aactcgtttt atcaccacac gttttggtaa tgtattagga
     1861 agcaatggtt cagtggtgct ccttttcaag aaacagattg aaaagggtgg acccgtcact
     1921 gttacgcata aagacattag gcgttatttt atgacaattc ctgaagcctg tagtttagtc
     1981 cttgaagcag gtgccatggg tcaaggaggc gaaatttttg tctttgatat gggtcagcca
     2041 gttaaaatat atgatctcgc cattaaaatg attcagttat ctggattacg tctcggtaaa
     2101 gatattcaaa tagaggaagt tggattacga cctggcgaaa aattgttcga agaactcctc
     2161 aacgatggcg aaaatgtaat tcctacctct catcccaaaa ttttgggtgc caaagtaaaa
     2221 atgtatcatc cagaagaaat cgagaaaatg atgttcgaac ttggccaaac cgtaattgaa
     2281 aatgattcat acaaattggt gaaaaaaatg aaagaatatg ttccggaata tatcagtaat
     2341 aattccatat actctatact cgacgctcaa cctcaaccct tcgttcagtg aaatagaatt
     2401 ccttttcatc gaataatttc tcaaaaaatt ttatccctaa gggttaataa aggaatttta
     2461 tcccgttttt atatgtctat tccgcaatat taatcaaaga agatataaaa agcatttacc
     2521 cctcttaact catcatcaag tctcctcgtc tctaatcccc tattctctat tctctattct
     2581 ctattctcta ttctctattc tccattccct attctccatc ctccatcctc cattctccat
     2641 cctccattct ccatcctcca ttctccatcc tccatnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnctcca tcctccattc tccatcctcc attctccatc ctccattctc
     2821 catcctccat tctccatcct ccattctcca tcctccattc tccatcctcc attctccatc
     2881 ctccatcctc cattctccat cctccattct ccattctcca ttctccatcc cccaaaaacc
     2941 tcatctctat tcactaaaat ttacctatat ggtgatttgc ccgaaaaatt tcaaaacatt
     3001 tctacttctg ttactatcga taagtactca aatactttcc tctcaaccag gcatagaacg
     3061 cagtataaat cctgattacc tcctccccga ccgttattta tatcatccag atgcaaattt
     3121 tcatacttca gtaagacctt atcttaggaa tgaagtcaat gaagtgatca attacgatag
     3181 catattgctc gaacaaccat taaaatctcc atggaagggc aagactggtg aatggatatg
     3241 gaaaaaagtc tttcatgaag atttgattca aatcacgggc aaagattttc attttgcagt
     3301 aaatccattg gtcaacttcg agataggtcg tgatttcaaa aatgatgata atgcttgggt
     3361 aaacacgaga ggatttatgg ctgaaggcaa tattggttca aaattcttct ttgctacaag
     3421 attttatgag aatcaggcaa catttatttc ctatatcgat aaggatattc aatcaaaatt
     3481 ggtgattcca cagcaaggtg cctataagag attcaagact gatggctacg atttttcgag
     3541 gtctgaagga tatatttcat ttagtcccaa taagtatttc aattttcgct tgggacacgg
     3601 taggaatttc tttggagatg gctatcgctc actgctttta ggcgacgcac atttccccta
     3661 tccatatttc cttatctcaa cccaaatctg gagaattaaa tacatcaacc tgtatgcgga
     3721 atttatggac agaaaagcac ctcattctga aagtttagga tacaagaaaa aatatggcac
     3781 tttccattat ttaaccacca acatcaccaa tcgattaaaa gttggattat tcgaagccat
     3841 catctggcag gcagaggata gcacaggcta tagaggattc gatttcaatt atttaaatcc
     3901 cattattttt taccgtcctg tggaattttc tttgggttcg cccgacaatg ctcttatggg
     3961 attgaatatc agttatacaa ttgggaaaaa gacagctttt tacggacagt ttttgctcga
     4021 tgaatttcag ttaaaggaaa taaccagtgg taaaggttgg tgggcaaata aacaagcatt
     4081 ccaattagga atgaaatggt ttgatatatt ccatgttgct ggacttgact ttcagtcgga
     4141 ggtgaatgtt ataagaccct atacttatag ccattctgat cccattacaa attacgggca
     4201 ttacagtgaa cctttagctc atcccttagg ggccaatttg gtggaatctc agaatttcgt
     4261 gcgttatcat catggacgct ggcatttgac ttaccaatta caaagagtcc aatatggagc
     4321 cgataccgca ggacttaatt tcgggaaaga catttttatt tcttatagta atcatccttt
     4381 tgattatgga aacaagatag gacaaggact taaaaccatt ctttggcggc atgatttcaa
     4441 agcttcgtgg gtcattaatc cggcatacaa tctaaatctc tcgacgggaa ttgtttaccg
     4501 ccaattaaaa acagatctca ccaatgatca aactttattt atttacatcg gactttccac
     4561 ttccttagag aaactttatt atgatttttg aagaaaaaat agagtggagg agacaccgaa
     4621 agacctttga gatggggaga tgcatgagac ttaaagaggt tgaaacgaga agacgaggtg
     4681 attgggaacc aatgcaaata gaaattcatt ttcctcaatt tacgatgggg agaagattat
     4741 ttttcttcat cactgattta catcgattta atccacagat ttatgctgat atttttctgc
     4801 gaatatctgc gagaactatt tttttctcgc tgattcacgc agatttaaaa acgctgattt
     4861 ccgctgattt tctgcgtcta tctgcgagtt ttattctgcg ataatctgcg agagatgtat
     4921 tttttctcgc tgatttaccc agattatcaa ctgataacta ataacttaat gttgacaact
     4981 taaaacaaat aactgataac tgttaactgt taactgacaa ctgtcaaact ccgatcccca
     5041 tcccccagcc accatcctct aaataccttc cctctcgcat gatgagaaaa cttatctttt
     5101 ttatttattt aggtttaatc actactctaa gtttaatacc atcgagtgat ttacccagtg
     5161 taaaattatt tagcggggcg gataaaatca ttcattttct gatgtacaca ggttttactt
     5221 tcttgttgat gtgggcttgg ccaaattttt tcagaggcag cttgttactg cttcccttgt
     5281 ttgccgtgat gttttatggt ttaggactcg aaattctgca agaccttggg catgaaggac
     5341 gagcatttga aatatacgat atccttgcca acacacttgg ctatttccct ggattggctt
     5401 tattctacat cgtacgtaaa tacgaaaatc attaaatacc ggctctttcg atatgtggag
     5461 gatttcctaa atataccaga tacccttcta ctgaaggata tccagctgcc aataaagttt
     5521 ctatatattt atgcatttgc ttttcatatt gaggatgagg ctgaccggtt ttataatcgg
     5581 ctactacagt aaaaccatct ccgaggatga taaggtcggg gcgaagccat ttcccatcgg
     5641 gtaaaagaat ttcccgttcg gttaattttt ctccctgacg ttcgaaaaca gctgaataca
     5701 gcggatcatt ctttaaaatt cgtaaatttt ctatgagggc ggaaccaaaa gtttcgccca
     5761 ttttttttgt catttggtga aactcgggaa tatttttcaa aacttcagac tcctgcgaat
     5821 ggtccaaaag cagatgaatc atcttaccca actctcgttc ggtatctggt gaagaagctt
     5881 cccatttttg tggcgctaaa ggcctgatgt gcatccttgt cgaaatgaca ttttcttcaa
     5941 tctctttatc tccaagtctc tcaatatctt cgtccattgt tttatctttc tcttcgtgcc
     6001 cctcgcaatg cgtctcaata gtcttaaaat tttccaatct cttcgtctct tcctctccca
     6061 atttcccagt cacctcatct tgttgctttt tagtggcatc ctcagaatat ataaaaattt
     6121 gtccgtcttt aaagcaaaaa tgctcctcca aaaccttctt gctctccttg ggcaaaagat
     6181 tagaatcaga atcagaggaa gatggagggt aggcgaaaat ataaagtctt tctttcgcgc
     6241 gggtaaaggc cacatacatc aaattgaggt cttctattaa ctgtttttct attaggtcgt
     6301 tataaaattt ttcaagacct tgaattccag aatccttgaa tttagatgaa gaatcgagga
     6361 gtagccaggg gatgttgaaa tttgaccaaa agtctctgtc aatgccgtct aaaatttcag
     6421 aggttgcgac ccattccgtt gaattataat taggtcgaga aaacaaataa ggacgaatca
     6481 ctaccggaaa ttcaagtccc ttagctttat ggattgtcat gattcgaatg gcgtccatat
     6541 cctcaggaaa gcttactgcc catgtatcct ggttttgatc ccattcgtcg agaaattctt
     6601 ccaatgaaaa tggtgtgtat cggttttgtc cctttatctt ttcgaggaat gcctgacaaa
     6661 atatatccgg aggatcgttc aattgacagt ccttcatgat tttttgggta agagtataaa
     6721 gatcgttaga gaaaagagta gaagaatacg atccaaaaac atttttaaga atttcctcac
     6781 cacttttttg caacaaattt ttctccctat tattttgggt caaatgcaaa agttcttttt
     6841 cttctggcac gtccttcatt ccttggttca attcgattgc cgttgccagc gcaagccgat
     6901 cgtcgggctg agctattagt ttgagccaag aaataactcg acgaacactt tctgattgat
     6961 taagcatgag agaatcgggt gaaaccacgg gaatgttttt ttggatcaaa aaatcagcga
     7021 tgcgttgggc ttcgtcattt ttccgaacca aaattgccat ctcattcaga gaatattttt
     7081 tatcggagtg attaagcaat tcattgataa tttgagaaat ccttttcaaa tgattatccg
     7141 aatcatcttt cggatcattc tttgtcaccc attcccattg taccaaacct ccctggcgac
     7201 ttttgtcggt ctcttgtttt gtatctctat aaattcttcg ggcttcttca cccaaacttt
     7261 cttttatatg actaaaaaat ttatgattga aatcaactat actcttcaaa gaccgaaagt
     7321 tctctttcaa gactttgggt ttttgagaag aaaatgaggc aaatagatgt tctgcttcgt
     7381 cgaaattttg ttgctcaggt tttctaaaaa tttgaggcag cttttcaaaa atctcgagtt
     7441 caccattacg ccagcgataa atactttgtt tcccatcgcc cacgattacg attcgcgcat
     7501 atccatcttc gcctcctgat aatgcattat gtagtaaagg gactagattg tgccattgct
     7561 gaacggaagt atcctgaaat tcatcgagta aaaaatgctt gaaacgcatg cccaacctca
     7621 cgtaaatata aggaacaggt tcagaacgaa tgactttaga tatcaatcgg ttgaaatcac
     7681 tgatatgcaa aacgttgtta tcgagcttga tctcctccag ggtacgatat aaattggaat
     7741 acaagggcaa acgaaacagt gaatcataaa caatgctggt gagtaagtat tctcttttca
     7801 aacgaatcaa ctcttcgaga tttttcttta tctctgcctt aatgggccgg aaattgcttt
     7861 gttttgtatc gtttttaccc gtaaaccata catcattttc aagagcctca ttcactgact
     7921 taaaagtagt aatattcttg atatcttcct cggtgaattt ctttacccat accaggcaac
     7981 tgccttttga gccacccttg aaatcacttt ctgaaagacc agtacttgca attttttgta
     8041 gaacatcctt tgctatgcgt tgggcttctt tttttataca tttggaatac tctctcacat
     8101 ttgaagatat ttcctgaatc tgttcaggag tataagttcc caattcactc aagggttcat
     8161 tcgatttctc gagaaagatg atttcggcca tttctcggat ttctctttcg atgttccagc
     8221 ccctttcatc atccaccatt tcgaggagaa tattttgcag taattccgta ataacttttt
     8281 ctctcccaac tcgatcgagc aagcgttgaa tggcctcatc gatcaataca tcctcgttaa
     8341 gttctacctc gaaatcatgt gcaattttca tgtcgaaagt aaatgaattt accactctat
     8401 acatgaatgc atctatggtg gaaatggaga aatagccata ataatgtaaa atattttcta
     8461 aggccttcct ggccttttgt tgggtttgtt catctgaact gttccatccc tcatttttta
     8521 gattttccaa aatgctgact ttgagcttgc catcattaca atttggcaac ttagccagca
     8581 aacccagaaa atacaatatg cgttccttta attctttagc cgccttattg gtaaatgtta
     8641 agccgagaat tcgacgaaaa acttcgggat cttccaaaac aagcgagaga aaatgtatgc
     8701 tcagcaaaaa ggtcttaccc gaaccggccg aggcgcgata aatttcgaat ctgggtgtat
     8761 tcatgagtca aaagtaagga gaattgttca tgaaacttag tgagaatacg aaagaatgaa
     8821 aaattttcga gatgaacgta tcgcaaccat tttcaatgag taaatttgcg acgattatgg
     8881 tagctctcga agaagcgata gaaaaagtga tgaatgaagc cagggtgctt ccaatggaag
     8941 aaatctccct taccgaggcg cagggtctcg tactggccga agatgtattt tcagacctcg
     9001 atatgccgcc tttcagaaaa gcagccgtag atgggtttgc atgccgaaga gaggatttgg
     9061 ataaaccctt gaaggtgatc gagaccatag ctgcaggaga agtgccacaa aaatccatcg
     9121 gagttcaaga atgtacgcga attatgacgg gagccatggt tcccgaagga gccgattgcg
     9181 tcgtcatgat cgaacatacc attggagaag atgagggtac gattcgcttc attggcgata
     9241 aaacctccga caacatcgcc caaaaaggag aagacattcg aaagggagaa aaagtattga
     9301 gtgctggaac agttctcgag gcccagcata ttgccgtact ggcttcggtg ggtagattta
     9361 ctcctaaggt gtatagaaga ccactggttg gcataattgc tactggcgac gagttagttg
     9421 aaccatggga acaaccgaaa gccggtcaga ttagaaatag caatgcatgg caaaccatca
     9481 cacaggtaaa gaaaaccgga gcagaatata tttatggcgg catagtcaag gataatccac
     9541 aggcactaag tgaaaaaatc aatctgttca ggcagaaagt cgatgtgctc ctgttttcgg
     9601 gtggaatctc tatgggtgag tacgattacg ttcctcaggt gatgaaagat gccggattgc
     9661 gtcttatttt caaagatatc gacatacaac ctggtcgtcc tactctcttt ggcatattag
     9721 accatatcgc cgtatttggc ttaccgggaa atccggtttc cagcttcgtg cagttcgaac
     9781 ttatggttca tcctttcatt tatacctcca tgggtaagac ctttcatgcc catatcaggc
     9841 atttaccaat aggagaagac ttttacagaa ccaaagccaa tcgtcgctca ttgattcccg
     9901 tgaggataga aaatgattgg attttcccgg tgaaatatca tggctcggct catatcaatg
     9961 catacacatt tgcttcagga tggatcgata tccctaaagg aaaaaaagaa ttgaaaaaag
    10021 gagaaatgat agatgttaga ctcttataga cgcgaaatta attatttacg tatttctgtt
    10081 accgatcgat gcaaccttcg ttgtcgttat tgcatgcctt cggaaggttt tcgttggatt
    10141 ccacacgaaa atatcctaag attcgaagaa attatagaaa ttgtgcgaga agctgtaaaa
    10201 ctgggtattc acaagataag ggtgaccggt ggagagcctt tacttcgaaa gggaattgta
    10261 aacctaatag agatgttagc cagcatagaa ggggtacaag acctttccat gacgaccaat
    10321 ggaactcatc tggaaaaatt tgcccgagaa ttaaaacaag ctggattgca gcgtatcaat
    10381 atcagcctcg atactgtcaa tgctgaacgt tatgcatatc tcacaggagg aggcaatatc
    10441 gaaagggttc ttcgcggcat agatgctgcc ctcgaagctg agctaacccc gattaaactc
    10501 aattgcgtaa tacaacacaa cagcaacgaa attgatgccc gaacggttac aaaatttgca
    10561 aatcaaaaag gcttagaaat tcgttatatt catcaaatga atctggcgga aggaatcttc
    10621 tcggccgtcg agggaggcga tggagggcat tgcgagcaat gtaaccgcct tcggcttacc
    10681 gccgatggtt tcataaaacc ttgcctgttt tccgacttac gttacgaggt tagacaatta
    10741 ggcgcacgca atgctctttt ggaaaccatc cgcaacaaac caaaagaagg tacaaccaat
    10801 cgaataaatt atttttacaa tattggagga taaactcaag gcacaaatct tgtttaaaat
    10861 cgcatttaga atatattttc gaggagtttt agaatgcagg acttagaacc ttacaaaagt
    10921 tggcattatc tttataatgc agcgcgcgac atacgaagcc cgttttttgg gagaagctat
    10981 cacaagacat tatgtaccca tactatttac aattattata tacatccaca atgggatgaa
    11041 attggctcac ccacactcta catcaaaatt ttatttgccg attatatcag aagattttgt
    11101 gtaatcgaaa tgttgggcga atggaacgat tgcatcaaca acgatatcat gtatttgaaa
    11161 cggaatgtgg tcgacgcact cacatccgaa ggaatcaggt attttatttt gataggagaa
    11221 aatgtgctca attatcatag cgatactgat gattattatc aggagtggtt cgatgacatt
    11281 gaagatggtt atatcattgg actgaatttt cgtgagcacg ttgttcagga atttatccat
    11341 gcccggctcg ataattacat gctttttggc ggtcggttca gtgaaatacc ctggcggaag
    11401 ttctcgcctc agcatttgtt cgatttaact aagaaaatga ttacacgaag actgagtgaa
    11461 tgaaaaacgc ttttaactcc tattcttcat tccctaaatc ccattctcta aaattcaacg
    11521 cattatacaa cacctaacat gtaagggcaa tactgtaaaa aaatcttaca atcctcagct
    11581 tcagtataga attttcgaat cataattcgt acctttgtat tatcagaatt ttaatttaat
    11641 tcattttcat cttatggtta aacattacga catcaccttg ctttatgaga gtcaaataga
    11701 aggttttcgt ttttccatga tggaaaaagc cctgcgattg aatattaccg gttatgtaca
    11761 acgccaactg aataacagtg tatttatcga agctgaagga gaaaaaacca atctggaaaa
    11821 atttttagaa tggtgtaaaa aaggaccact gggagtcaac attctaaggg tagaagttac
    11881 cgaaggagaa gtgaaaggct acagcacttt cgatattcaa catcgctaag gtaattcttg
    11941 caaaaaggtt ttctgcaaga gcttccaaac atgacgatct ataggaaaag tcatcacctc
    12001 gtcagactca tgcaggttta gcattctgaa agtttgggct ccctcttgtt caacagaaaa
    12061 atcaaaaggt ttatctacaa gagaaatttt ccgggggaaa ggtaaatcga tgagataata
    12121 aatggaaaga agctgtgaag gcggttctac cagcaaagta cgctggaaga aatcggtagt
    12181 ataaaaatgc tttagaatga caggctcctg atggagttct tctttaattt cgcggcggag
    12241 gccatcgagg gttccttcac caaattcgag cccaccaccc ggaaacttag tcatcctcat
    12301 gccgaggcaa aattcgtcgg taaccaaaac agaagtggca ttcatgtaaa ttccatacac
    12361 cctcacgtta aactgctgta tagatcgagc agaaaaattc ttcctcattt tcttgccagt
    12421 gttatttctc tttttcccgg tggccccgga actttttcca ctaaaaatcc acatgcactc
    12481 aaattccttc tcacttgtcc cttggtgctg taagtaacca gaattccacc ccttgacaag
    12541 gcattgtaca aaaattgcat ggcttgcttt tcccataatt cagggacaat ttcaggccga
    12601 aaggcatcga aaaatacgca atcataagat tcatcgggat aagaaatctt ccggaaatct
    12661 tcctgaatca cctgcaacga aaataattca tctatcttta ttactcgaga accatcgaaa
    12721 gaagacaata ttttcgcata agcttgttct gcatagggag atcgacaata aacaggataa
    12781 tttagccctt taagtaagga aggaggcgga aggaaaggat cggttccagt ataagaaaca
    12841 ggcaatttat gaagttgagc atagtcccaa gttaacagtg cattaaggcc tgtaccaaat
    12901 ccaacttcta atatatgtat agtttgcctt gccgatgcta tacttccaag gcctgtatga
    12961 atgaaaacgt gcatcgcttc ctgcacggct ccatgcaagg aatggtaagg ttctgagtac
    13021 tcctctatcc ataatgtagg tgaaccatct tcagtaacaa cgacctcagt cataaaaatc
    13081 ttattcgaat catagatact catattacaa agataggctg cttttatgag gtaaaaattc
    13141 atcaaaatgc tgtaatttta ttcccataaa atttcattca agtgatagcc gataaaattc
    13201 tttttctgga tatcgagact gtgcctcgat ggccagacta tgacgcaatg cctgaacatt
    13261 tcagagtttt atggaatcat aaagcaaatc atatcaagaa tctccctgac gacacggctc
    13321 gttctctcta ccccagggct gggatctatg ccgaatttgg aaaaatcatc tgcatctcta
    13381 ccgggttttt tcatcaggat tgttttagaa taaaatcgtt ttatggagac gacgagaaaa
    13441 ttatgctcga agatttcaag accatgctcg aacggatgaa cagcactcaa ccctatctgc
    13501 ttgccgctca caatggcaag gagttcgatt tcccttacat ttcaaggcgt atgctagtaa
    13561 atggaatcat gctaccagat gttttcgaca attcgggtaa aaaaccctgg gaaaccagtt
    13621 tcatagatac catggagatg tggaaatttg gcgattataa acattatacc agcctcgctc
    13681 ttctggcggc tctcttcgac attccttcac ccaaggaaga tattgacggc agtcaggtag
    13741 ccatagtata ttatgaatcg aacgatttga aacgcatagt gcggtattgc cagaaggatg
    13801 taattactgt tgcgcgactt tactgtaaat tcaaaggttt gccagctcct gccgatggcc
    13861 agatagtcat tatcgaatag cccgaaacct caattatatg gcaaaaacgg aattatcgaa
    13921 gtcaacattt atcagagcat tacaatgtct caaatcggtt tatttgaaca agaacaggcc
    13981 atttttgaga gaccatctcg aagcacgtca actaaacaaa ttcgaacggg gtcatgaaat
    14041 cggttcatta gcctggaaac ttttccctgg tggcatagat tgctctccaa aacatcctgc
    14101 ggcttatgcc aaagcagcag ctaaaaccac tgaactcatt cgcagaggag caaaaattct
    14161 ttacgaagct gttttcgaat acgatggcat acgggttctc cccgatatcc tcgtgttgaa
    14221 cgattcacag atacgaatgt atgaggtgaa aggatcgttg aacatttcac aaacatatat
    14281 cctcgatgcc gcattgcaat attatgtcat cagtggagcg ggttatactc cttcacagac
    14341 ttttattctt catgtcaaca agaattatat ttatgaggga ggtgagattc atccagagga
    14401 tttttttgtt tttcaagatg ttactgccga agttattcaa caacaaaatt ttatcgaatt
    14461 aaagattcca gaagctaaag cagcggttaa tgctgataaa tcacctgaaa ttcccatagg
    14521 acctcattgt tatttacctt acccctgcga tttcatgggc ttgtgctgga aatcgatccc
    14581 agccgacagc ccactctatc tcaacagcat cgacattcac tcccgttttc ggcttgtctc
    14641 agaaggatat tcttctattt ctcgaatccc tttggatagt ttagataatg aactgctgaa
    14701 aactgaagta gaagcaagga taaatggcaa gcctatatac aaggcatctg aattgcagaa
    14761 attattgcaa cctcaaaaac atcaggttta tttgaagatt ctgttcatcg aaaatgcagt
    14821 gccacggctt gcaggaacgc gtcctttcga gcctcttcct atagcttgga tagccgtgaa
    14881 ggaaaatgga agtcttatca tgggcgattc cgaaaatcag tatcaagtct tctcggaaat
    14941 catcaacctg ctcagaaatt ttctgactga agatgatcaa ctcgtgtttt ggggaaaacc
    15001 tatggagctt ctcatccatc aggtagaatt cgatctccca ttcttaaatc tcgaatctgt
    15061 ctttcaatcg gaaaatcttt atctgccatt ttatctcgaa gaaaaaacac ccgaaaatat
    15121 tgcttcacag ttgggaatta tcaacgacga aatcacaacc atgaacgatg acgaggcgat
    15181 aagagcctgg tcgggtgaat catcgaaggg aaaattcaac tcccgggaga tcgaaaatca
    15241 tttgaaaatg caattatttt tattgagaga attgtatagg tatgctgctg gtttcatcga
    15301 aacagatcat tctattgaag ccaaaaagta ttaacaatga acttgaacaa aatttcgatt
    15361 attcgctatc attattcgac cagatcatag agaatgacag ccttgcaaat caccatttca
    15421 ggacgagttc agggagtagg gttcaggtac tacatcttta gagcagctca ggaacttcga
    15481 attaaaggaa tcattaaaaa ccttcccgat ggtaaagttt acatcgaagc agaaggcgac
    15541 cctcaaaaca tagaaatatt tctggaaaaa tgcagggaag gccctcccct ggcttatgtt
    15601 actcatattc atattaccga atgcctaccg tctggttata acgatttccg cgtcgcttat
    15661 ggatattgaa aaattaacat ttccaacgaa agttgcgaga aatgaaaaaa aaaataatat
    15721 ttttgttcaa aatatcttag atatatccgc gggctgcaag agccgataga cctcacaggt
    15781 cataaataca gtctgaacgt ttatcaaatg taaagagaga aatcatgaaa aaagaatgga
    15841 agaataaatg ggattctatc gttaagaagg ttttctctgt cgagagcttg cccgttcaac
    15901 cactctggct gaatttccaa cgcaagcagg atgaagaaga gtttaccaac cagtattaca
    15961 aaaatattct gacacgggta cgagtatgga tgctgatttc aacctcagga atactcctcc
    16021 tgcaatttat tgattattta ctcaccggta agtttatgaa cgacgctttt gccattcgct
    16081 tcgagatatt tttgcctttt tccctgcttt ttattctcat tacttttaca aatttatata
    16141 tcgatttttt tcagtatctc aacttgctct ggatttttat gacgagtttg ggaggaatca
    16201 taaccgccat cttatgtcct gaagcttcat tgccatttat tttagcctct atggctctat
    16261 tttttatagc ttctttcgtc ctgtttgggt taaagccata ttttgctctt ataggaaata
    16321 ctatattggc aattggtttg ttgtggattt tgatcaatca aaaaattctc catccttcct
    16381 atacatggcc aattattatt ctcctattca tctttcttat cgtaggttat tatgcaggct
    16441 ggaagataga actactggag cgaaaactct attggtcggt taaaaaacaa aagagctttt
    16501 aatgaaatcg catgcctcga atattgaaca tcctcattag ccaatctacc ctatatctga
    16561 ggatcaacca caggataatg ccaggttttg tcagctaaaa aaacaaaata agaatagata
    16621 ttccttagca tttgccataa catttccata gataaagcac gagttgaatt tgtatatgat
    16681 ttttgcatag agggtcttca gggataatct ttcccagcta ttatctgcat cgaagggata
    16741 cgatagacga actgaagcag aacgatgtat tatttaaccc aattgggatg ctttaccatc
    16801 ataaattaaa aattttcata gaactttgct tctcaattta ccaaaaagca aacacagtgt
    16861 ttagcaatcc tttagaaaat atttctccca tagatgggcg atatgcaggg attacctggc
    16921 ctctgtcgaa ttatttcagc gaactatctc tgataaaata ccggacaaga atcgaaatag
    16981 aatattttat tgcactttgc cgactaccct tgtctccact caagaatttt ccgagggcag
    17041 gattccaatc tctgagaaaa atttaccaga acttcaatca aaaagatgcc gaacgggtga
    17101 aggaaatcga aaaaaccacc aatcacgata taaaagctct cgaatatttc ctgagagaaa
    17161 ctttcgaccg attgggtttt gccgaatata aggaattcat tcactttggg cttacctctc
    17221 aggatatcaa caataccgct cttcctcttg ctctgaaaga agccatttcc aatgtcatca
    17281 gacaaccttt gatagatatc ttatctcaac tacatacggc agcccgaagc tggaaaaaca
    17341 ttcccatgct ttcacacact cacggtcagc cagcttcacc taccacactg ggcaaagaaa
    17401 tctttgtatt tgtcgaacga ctcgaaaaac aaatcactca tctcgattat caatctattt
    17461 ctgcaaaatt cggcggcgcc aacggtaacc ttaacgccca caaggtggct tatcccgaaa
    17521 tcgactggat aacttttgcc aacagtttcg tgaatgatgt tcttgggctc gaacgccagc
    17581 aaaccactac acaaatagaa cactatgatg atctggcctc tcttttcgat aattttgccc
    17641 gaatcaatac aatacttatt gatttatgtc gtgatctctg gttgtatatt tcgatgggat
    17701 attttcttca gaaaataaat gaacaggaag taggctcgtc aaccatgcct cataaaatca
    17761 atccgataga tttcgaaaat gccgaaggca atttgggatt ggccaattcg ctatttgaac
    17821 atctggctcg taaactgcca atctcacggc ttcagcgcga tctcaccgat tcaacagtta
    17881 cccgaaatct gggcgttcca ctggcacatt cactcattgc ttaccattcc atcctcaagg
    17941 gattcgataa gatttatccc aacgaaacaa aattgaagga ggacctcgat accaactggg
    18001 ctgtgattac cgaggccatt caaaccatat tacgccgcga aggatatcct gatgcttatg
    18061 aaaccttgaa aaaactcagc cgtaccggga aacccatcga tgaacagacc atcaaggaat
    18121 tcatcgattc gctcgaaata gccgaaccgg taaaagtcga actgcgacaa attactcccc
    18181 ataactatat cggaatcatt tcattcgaat gaaagtcagc ggactcacca ttgtcagaaa
    18241 tgccgtacaa ttcgactatc ccgtagtaga agccatactt tcaatcttac ctctatgcga
    18301 cgagtttatt gtaggtgttg gcaatagcca ggataatacg ctcgaattgg ttcaacaaat
    18361 agcttcacct aaaatcagaa tatttcaaag catctgggat gaaaacttac gtcagggagg
    18421 gcgagtattg gccgtggaaa ccgataaggc tttccagcaa atcagtcctg atactgactg
    18481 gtgtttttat attcaggccg atgaaattct gcacgaaaat gatcttgata aagtttacga
    18541 aaacatgcgg aaaaatttat ccgatagaaa cgtggagggg cttttgttcc gatatctcca
    18601 tttttacgga tcctacgatt atataggaga ttcacggaag tggtataggc acgaagtgag
    18661 aattgtcaga aactcgccca aaattcattc atttcgtgat gctcaaggtt ttagatggga
    18721 caaccgtccg ataaaagtca aagattcagg aggtacgatt ttccattatg gatgggtgag
    18781 acctccagag gttcaggcta agcggcaaaa agcctttcac cgattctggc acgatgacca
    18841 atggataaaa acacacctga cttcgaacga tttcttcgat tattcacaga tcgattctct
    18901 ttctttattt cgcggaattc atccccgagt aatgcaagat cgcattcgca gcatgaattg
    18961 gaaatttgat ttcgacccta cacagaaaaa tttcggattc aaggcaaaaa ttctttatat
    19021 catcgaaaaa atcttcggct ggagaccatt cgaatataga aattataggc tgctgaaata
    19081 aaaaaatgct acacagcaaa gggattgcct cggcaaaata tgcatttgcc ctttcttcta
    19141 aatagttaag gcaagtagcc tgctgactat ccaataaaga tatttattca ttacactgtc
    19201 gaaacatttc aacgtcaact tattgtatta cgccgtccaa tgaggcttga atgacatcgc
    19261 gcccatattt gttcaaagtg caaccgtgaa caagtgaaca aagtttgtcc ggatcccgtt
    19321 cgccacctat gatggccat
//
LOCUS       DAOORU010000024        18771 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_3584_length_18771_cov_5.538416, whole genome shotgun sequence.
ACCESSION   DAOORU010000024 DAOORU010000000
VERSION     DAOORU010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18771
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3584_length_18771_cov_5.538416"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLF20_02965"
     CDS             complement(<1..200)
                     /locus_tag="PLF20_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HOX80002.1"
                     /translation="MKKILPFIIAVIITLSGSAQQDNLFEISKNLELFFSVVKELQIN
                     YADQIDPGKLTSTAIKSMLNTL"
     gene            complement(309..710)
                     /locus_tag="PLF20_02970"
     CDS             complement(309..710)
                     /locus_tag="PLF20_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOX80003.1"
                     /translation="MRTNFRKYERLCSKKYIENLFKKGESFVVYPLRIYLTTAHPVAY
                     TGVQVVFAVPQRNFRKATQRNLLRRRLRESYRNNKQIISNLLYSTHFTIHIGFLYIAK
                     EIENYSSIEEAMIKSLQLIAEKYKNSPIEEK"
     gene            complement(742..1698)
                     /locus_tag="PLF20_02975"
     CDS             complement(742..1698)
                     /locus_tag="PLF20_02975"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOX80004.1"
                     /translation="MKIKKVLISQPAPAEGEKSPYNLLAQKYNFEIVYQKLFKVEGVS
                     SKEFRRQQIRLLDYDAIVLTSRQAVDHLFRLAKEIRVEIPETMKYFCATENIALYLQR
                     YIQYRKRKILHGHQSLSDLADLMKKHKDDKFLIPCSDSSKNEVPSFMDELEINYKKAV
                     IYKTVPRDLHSLDIKSFDLIVLFSPMGIKSLFKNWPNYKQQKQLIGTFGDNTTKAALE
                     NGLRVDIQAPSATCPSMTMAIEYYILNGNKKTKSKNRSTAKKKEIVETCENSNNDEIA
                     PSAENFDDEICNSENIDEGNADDINVNPEPPLKKNHESPAMN"
     gene            complement(1757..2614)
                     /locus_tag="PLF20_02980"
     CDS             complement(1757..2614)
                     /locus_tag="PLF20_02980"
                     /inference="COORDINATES: protein motif:HMM:NF025459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4271 domain-containing protein"
                     /protein_id="HOX80005.1"
                     /translation="MPPDSVRAILIPFADTFAAINPLDTLKGFDAICVSKLSVISDTT
                     SDKPISSFFTSHTLISQNYGEQKPIAGLPPWISLVFLLSILLLIAAKLIHGQNLMQIL
                     KALFSRKKFNQLEKDIVSVVNPFKNTLLFNAVLMLGLLVFMILQEGNFRKMVFEWDIK
                     LFGSLLVFLLIFFLFKSLFISLIGSLIHRSQFSKKLLLYFSLILSIIGVSLFPILALA
                     IYLNEDLRRILLMISIAIVSILYLFQIIKWTQTAIETKGAFGIYFFSYLCTVEFLLQL
                     ILLKAYLLV"
     gene            2977..3981
                     /gene="pta"
                     /locus_tag="PLF20_02985"
     CDS             2977..3981
                     /gene="pta"
                     /locus_tag="PLF20_02985"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOX80006.1"
                     /translation="MNSFLEGVRQRARQLNKCIVLPEGEEERTLIAANEVLKENLARI
                     ILMGYPEVIRQKSKDLNLEHISKAEIVFPDAYEKRQYYADLLYEIRKNKGMTPEQAYR
                     LAADPLYLASLFIKDGKADGEVAGAINTTGNVLRPAFQIVKTLPGVSVVSGAYIMIFK
                     DSHWGENGMMIFADCAVNPDPTECQLAEIAVMSARTARILAGIEPRVAMLSFSTKGSA
                     THTLIDKVSHATEIAKEIDPSLMIDGELQADAAIVEEVAKRKCPNSPVGGKANVLIFP
                     DLQSGNISYKLVQRLGGADAIGPVLQGMAAPVNDLSRGCSSQDITNLIAITVIQAGGL
                     "
     gene            4527..5324
                     /locus_tag="PLF20_02990"
     CDS             4527..5324
                     /locus_tag="PLF20_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HOX80007.1"
                     /translation="MKALIFDIRRFCVNDGPGIRTTVFFKGCPLRCLWCHNPESQLNK
                     PEPFLRMDKLEGKFFEHEEQIGYEIDLDELLEILFRDRAFMEESGGGVTLSGGEPLWQ
                     WKFSAALAQKLSESGIHVAVDTCGYVQLRVFEAILPYTHLFLYDLKGYDNHRHKENTG
                     ASNSLILENLEYLIHQRANIILRYPVIPGYTDSEDDVRGILSIMKRNSDILREIHLLP
                     YHKLGQNKYRGLQASNPMPENIPDADESVGHIESIFKSNHFIVKIGG"
     gene            5328..7703
                     /locus_tag="PLF20_02995"
     CDS             5328..7703
                     /locus_tag="PLF20_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011965172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HOX80008.1"
                     /translation="MNTKITQRIEKLREKGLRAVNRISAERALLITQFYRTAEPYRVS
                     VPVARAMAFDYILSHKKIYIDDGELIVGERGPEPKAVPTYPEVSLHSLEDLRILNTRP
                     KVSFRVDEDTFAAYRDVIIPFWKGRSNRERIMGLMDEDWLRSYEAGIFTEFQEQRAPG
                     HTVGGSKIFNRGMLDLIEDIHRVQLKLDLYEDYLAFEKNEELKAMEIAARALIHFANR
                     HADLLEELSKQEEDLQRKSELKELAQISRRVPAFAPSTFHEALQHYWYIHLGVITEVN
                     PWDSFNPGRLDQHLFPFWQKDRNEGRLSDEKACELLEAFWIKFNNHPAPPKVGVTAQE
                     SNTYTDFALINVGGVKVDGSDAVNPLSYLILDVVEEMRILQPSSMVQISRKNPDQLLH
                     RAFQIIQTGFGQPSLFNTDAIIAELVRQGKSIEDARNGGASGCVEAGAFGTEAYWLTG
                     YLNLPKILEITLNNGFDPRTKVQLGPNTGTAESFENYNQLLNAFQTQLNHFIQIKIKG
                     NNIIEKLFAHYLPVPFLSLIIDDCIENGMDYNAGGARYNTSYIQGVGIGSLTDSLTAI
                     KYHVFDKKTLSMNNLIKVLENDFEDDLSLRHLLVYQTPKYGNDDDYADIEAQRVFEMF
                     FEAVDGRPTARGGKFRINLLPTTSHIYFGSVIGALPDGRRAGQPLSEGISPVQGADHH
                     GPTAVIRSCAKLDHLRTGGTLLNQKFTPSFFKNHEAIQKLTALVRTYFRLDGHHIQFN
                     VVNAEILRDAQREPEKYRDLIVRVAGYSDYFNDLTPELQEEIIRRTEHETM"
     gene            7789..9966
                     /locus_tag="PLF20_03000"
     CDS             7789..9966
                     /locus_tag="PLF20_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOX80009.1"
                     /translation="MEFEEIKTKADAKYLALVKVIKPYLHRNDTAVLRKAYETSLVHL
                     GEIINEARQPLVLQQLDIALILAHRMGMGPQTVLPAFVVEMLRFGKIEPTQISSTFGE
                     VTLKITQGLLSIEKLNLSKVSTGSENFRELLISLSNDIRGIFYLMARNLYDLNHYSSL
                     PTASQQLIIQLARDVFIPFAHRLGLYNIKTQLENDVMRYCEPEIYRDIAQKMEELRPS
                     QQSFFTDFIIPIREALSEAGIECEFKWRFKTIPSIYQKIKKQNVDIQHVYDLFAVRII
                     LTGQLSDEIAECWRVYSIVSNIYPPDTSRLRDWITRARPSGYESLHTTVMTPEGKFVE
                     VQIRTRRMDEIAEKGDAAHWHYKEAGRKSEIDTWLREIREVLESYPGEGADPLHTHQP
                     LPSSTHIFVLTPAGDVKELPQGASVLDFAFEVHSSLGASCTGARVNGKVVPLKHRLSN
                     GDKIEILSSKNQFPKIDWLQYCVTSKARNKIRRALSEDKLAEAETGKSALLRHCRKWK
                     VNYNDEMVEKLLKHFKMKTALELYASFSHSDIDWAEVKRILTENTLSEIHIVPPVKRK
                     KPVKEEPAPNAIIIGDESKNIHYSLARCCSPIYGDDVFGFITVNRGITIHRKNCPNAL
                     TLFSKHAYRVVEARWFEKAGEGPYQALLRITGDDRIGLLADITHVLTNELNVNILSVN
                     LDSKDGFFDGKMKVQVTNVAHLDSVIHRLMKVKSVARVKRFYD"
     gene            10029..10637
                     /locus_tag="PLF20_03005"
     CDS             10029..10637
                     /locus_tag="PLF20_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80010.1"
                     /translation="MKKINLAVCILCLGALSICSSSCKQNPKANSSGEVEKTNQQKSP
                     EFKYDDLDRIVQRKDFSYNRDGSVRYKNQYDYKYDDRGNRIEEITQSWDLSGKRVVYT
                     IARFHFNDLNLKIEAIFQSFDPEGREILWVKNVYDYDDNGYEIRDQQFDKQGRPLSLV
                     TRKRDEKGRILEETTTIYNPDGSIKEHKGAQYDINGRILKTW"
     gene            11095..11697
                     /locus_tag="PLF20_03010"
     CDS             11095..11697
                     /locus_tag="PLF20_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HOX80011.1"
                     /translation="MNYGSITILMAIESSFIPFPSEVIIPPAAYIASKPGSELNIILI
                     VIFGTLGALLGAFVNYFLALWLGRPLMYKLADSRLGRMLLLSSAKIQKAENYFNQHGN
                     ISTFIGRLIPGIRQLISLPAGLARMNLLSFTLFTALGATTWNIILALLGYIAHGQADL
                     IEKYNKELSYIILAIVGLVALFYILKYVRKRMKSTKQLPL"
     gene            11845..13380
                     /locus_tag="PLF20_03015"
     CDS             11845..13380
                     /locus_tag="PLF20_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HOX80012.1"
                     /translation="MIQKNNLRFSIFIVFIIDLILLFSYSSSAQQKRRILIKKADILI
                     SEEWHDTTVNLLRGHVVLEHEGALMYCDSAFMYEQLNSFDAFGNIHIDVSDTLDLFGD
                     VLFYDGNTRIAEVHYNVRLIDKTTTLYTEHLFYDRNLEIGHYPDSGRIIDKDNILTSR
                     NGYYFSRPREYRFRENVVLRNPDYVLVTDTLNYDTKTRIAYVKGPSTITGKERYVYAE
                     DGWYDTQKEMVSLKTHVFMRNKDRTLKADSVFYDDKISFGRAFRNITGHDTTYRVILN
                     GDFAEYSDSLGYVFFTRRARVLYYEKKDTDTIYIHADTLRLTFDTAQQAKFFYAFNHA
                     KFYRHDIQGMSDSMLYNYPDSMFVLYKIPVLWQDSNQLTADSIKIWVANQKVDSMTMY
                     NTAFIIQRDDSGTFNQMRGRDMFAYFVDGEMRKVFVKGNSETVYYIREEDGSLIGINL
                     AVSSNMVLLIEDNELTDIIYLIKPEGGTYPEKQFPADRLKLKGFEWHEQRRPRKPDDI
                     FIW"
     gene            complement(13384..14331)
                     /locus_tag="PLF20_03020"
     CDS             complement(13384..14331)
                     /locus_tag="PLF20_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOX80013.1"
                     /translation="MKISKEFKTGLTFIGVIIIFIWGYSFLKGNGIFHNKKFYYVVYD
                     QAVGLAPTSPVFIRGLQVGKVSDVFFHKNMNGSVVVKLNIVNQFPIPNNSVATIINRD
                     LMGAKAIEIILGDSPHRAQPGDTLAGAIDAGLLEGLNRQLTPVMQKAELALNSIDSTM
                     KVLQVSLNHENRENISKSLQSIQFSLRNIKQITATLDTLILSNKSRVSFIIQNIESIS
                     ANLESNNEQLTAVISNIHTISDTLAQVHIEQTFKKLDYNLELLSEILIKIERGEGSAG
                     LLLSSDSLYDNLSRSTKELELLIRDIRENPSRYLKFSVF"
     gene            complement(14412..15668)
                     /locus_tag="PLF20_03025"
     CDS             complement(14412..15668)
                     /locus_tag="PLF20_03025"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOX80014.1"
                     /translation="MKKNKLPKKYWASKSKNLISTFRGLFCLVMVASMIISLNDEVLA
                     LSKDGKIRTVVIDAGHGGKDPGALGKTSAEKNITLSVALKLGQYITKNHPETKVIYTR
                     SDDSFVELYKRADIANKNKADLFISIHCNASKSRIHYGAETFVMGLHRSQANLEVAKL
                     ENAAVLYEENYQTRYDGFDPNTPETEIIFSMYQNLYRQQSLLLASNIQNQLNTVASRF
                     DRGVKEAGFLVLYHTTMPSVLVELGFISNENEENFLKSKAGQEKLATSLASAFNAYKM
                     EVENIPAISSMAPVKPDITVDSELLSSTADQQNKTLQTSSFAQQKNTAQAISSSPTET
                     IASTSSPIIYRVQITASTQPLSKNHSFFKGLKDIWEYKHKGYYKYTSGHFFNEDDAIQ
                     YRDSLRKQGFKDAFVVPFRGDQRLNP"
     gene            15748..18483
                     /locus_tag="PLF20_03030"
     CDS             15748..18483
                     /locus_tag="PLF20_03030"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOX80015.1"
                     /translation="MKVFPKDINNLVHILVGTHFLGDLSSTFWKNIFIFIVFILILGN
                     SLSPQNIVRDTLKNDSLILFDTKNFISPIGDTLPERDSLIIDTNQVVPKPKSSKNVLE
                     DIVNYKCNDSIRFRVKEQKVYLYKDVEIAYQKMGLTADYVEVNFRLNEIFASGWPDST
                     GKIRGTPVFTEGDQSFKASKIRFNFKTNKGLIENVITQDGEGFLHGTIVKRMPDDVIY
                     QKGGMYTTCNLDHPHFAFQFNKGKVIPGDKIVTGPAYLTIEDVPTPLFVPFGLFPNKK
                     GQRSGVIIPSYGESANRGFYLERGGYYWAINDYMDFTLTGDIYTRGSWKINPQLRYAK
                     RYKYSGNIDFARACNISGEPGDPGYFNSRDFQFRWSHRQDPKANPNSNFSANVNIVSN
                     KYNHFNPNQMNDYFSNTFQSSISYQTKIGQNYNLTMNANHSQNTLNKTVNISLPQITL
                     TGNRFNPFKKKNRIGKVKWYENITMSYSMNAENRINTYDSLLFKHETLKNMQNGIKHS
                     IPISSSIKVLKYFNLTNSINYTERWYFRTIHKAWVDTTDWSHDTVASGYVRTDTAYGF
                     KCARDFTFSSSLNTRIYGMFTFMKGPIVAVRHVVSPSVSFSYNPDFSSSFWGYYKPYQ
                     KNAKGTIDKYSIFEGGIYGSPIGSKAGRISFSISNNLEMKVRNRKDTLTGVKKIPLID
                     NLTISTSYDFMRDSLRWSKVQMSGRSTLFKNITLNYSSSWDPYILDSTGNKNLNQYEW
                     KVNRRPLRLDNTNWNLSLNWNFSSKSKGSGGKPPLNPNDPEYQDYLLNPDSYIDFDIP
                     WKLNLAYTLNYTANHKYPYYKEEVIRKIVQTLNVSGDISLAPRWKIGFSTGYDFETNK
                     ISQASLNIYRDLHCWEMRFNWIPIGYLKSWNFQINVKSPMLQDLKLTKKKDFRDR"
BASE COUNT         5727 a         3530 c         3827 g         5687 t
ORIGIN      
        1 tccagagtat ttagcatgga tttgatcgca gtagaggtca gcttaccggg gtcgatctgg
       61 tcggcatagt tgatctgaag ctccttgacc acagaaaaga aaagttcgag gttcttcgaa
      121 atctcgaaaa gattgtcttg ttgggctgat ccacttaaag ttattataac cgcaatgatg
      181 aaaggtagaa tttttttcat attacttgga gtaaatatat ttttttcttg agtgatgaaa
      241 ctgaggaaat atgaaatttc ctttgggata acgttgtaaa tgttgtttga ttataaggag
      301 agttgatttt atttctcttc gataggggag ttcttatatt tttctgctat gagttgcaga
      361 ctcttgatca tagcttcttc gatagaggaa taattttcga tttcttttgc aatatacaaa
      421 aaacctatgt gaatagtaaa gtgagttgaa taaaggagat tggaaatgat ttgtttgtta
      481 ttccgatagg attctcttaa tcggcgtcga agaagatttc gctgagttgc ttttcgaaaa
      541 ttgcgttgag ggactgcaaa aactacttga actcctgtgt aagcaacagg atgagcagta
      601 gttaaataaa ttcttagagg ataaacgaca aaggattcgc ctttcttaaa tagattttcg
      661 atatattttt tcgagcagag ccgttcatat ttacggaaat tggtgcgcat ttaagtacgg
      721 ttaaaaaccg ttggcaaagt ctcagttcat ggcaggagat tcatgattct tcttaagggg
      781 aggctctgga ttgacattta tatcatcggc attcccttca tcgatgtttt cactgttgca
      841 aatttcatca tcgaaatttt cggcagaagg agcaatttca tcgttattgg agttttcgca
      901 ggtttcgact atctcctttt ttttagcggt tgaacgattc ttggatttgg tttttttatt
      961 tccatttaga atataatatt caatagccat agtcatagaa gggcaagtag cagatggtgc
     1021 ctgaatatca actcttaacc cattttcaag ggctgctttg gttgtgttat ctccaaatgt
     1081 accaatcagt tgtttttgct gtttataatt aggccaattc ttaaataagg atttaatacc
     1141 cataggactg aaaagcacga tgagatcgaa gcttttaata tcgagagaat gtaaatcacg
     1201 aggaacggtt ttgtagataa cggccttttt gtaattgatt tccaattcat ccataaaaga
     1261 aggaacctca ttttttgatg aatccgagca tggaataaga aatttgtcgt ctttatgttt
     1321 cttcatcaga tcggcaagat cgctcagaga ctgatgccca tgtaagatct ttcttttcct
     1381 gtattgaata tatcgttgta aataaagtgc aatattttcg gtagcacaga aatatttcat
     1441 ggtttcgggt atctcgaccc ttatttcttt agcaagcctg aatagatggt caacagcttg
     1501 gcggcttgtc aaaacaatgg catcataatc gagcaatctg atctgttgcc gtctgaattc
     1561 tttcgaactt accccttcta ccttgaaaag tttttgatat acaatttcaa agttgtattt
     1621 ttgggcaaga aggttgtaag gtgatttttc tccttcggct ggggccggct gtgagataag
     1681 aacttttttt attttcaatt cttttaagtg tttaagtgaa taaaacgaat attttattaa
     1741 cctattcttc ttaaaattaa actaataaat aggcctttaa tagaatcagc tgcaacaaga
     1801 attcgacggt gcaaaggtac gaaaaaaagt aaatcccaaa tgcgcctttc gtttctatag
     1861 ctgtttgtgt ccattttatt atctgaaaca aataaagtat agatactatt gctatagaaa
     1921 tcatcaataa gattcgtcgt aaatcttcat ttaaataaat ggccagtgct aagatgggaa
     1981 acagggaaac accaataatg gagagaataa gactgaaata taaaagcaat ttctttgaaa
     2041 attgactcct atggattaag ctgccgatca agcttatgaa taatgatttg aaaagaaaga
     2101 agataagtaa aaaaaccagc aaggaaccaa acaactttat atcccattca aataccattt
     2161 ttctaaaatt tccttcttga agtatcatga agaccaacaa gcctaacatc agcacagcat
     2221 taaaaagcag ggtgttttta aacggattca ctactgatac gatatccttc tcgagctgat
     2281 tgaatttttt ccgagaaaag agagctttga gtatttgcat taaattttga ccatgaatca
     2341 attttgctgc aataagcaat agaatgctca aaaggaaaac gagagaaatc cagggaggga
     2401 ggccggcaat tggcttttgt tctccataat tttgggaaat gagtgtatga gaagtgaaaa
     2461 acgaagaaat tggtttatcg ctggttgtat cgcttataac acttaatttc gaaacacaga
     2521 tggcgtcgaa tcccttcaac gtatctaagg gatttatggc tgcaaaagtg tcagcaaaag
     2581 gaataaggat tgctcggact gaatctggcg gcatagtgaa gtttaccagc taaggtttgc
     2641 aaaattactt cgaaattgga aaggctgccc ttctatatgc aaatagtttg tttaagatta
     2701 ccccaggcag ccggtaaaat gaacataatg gaaaattcgg ttaataacaa gcgtcaaaat
     2761 atttgaaaat atggaatggt aataaagtat ttttacttct ggatatctgg attcatcgat
     2821 acatttcgga tattggagta gggaaatagg tgagtttgac tttttttcag aaaacattat
     2881 gggggaaatg agagtcgttt ttttaaagtt tctaattttg ctttaaaatt tttggagcag
     2941 atttttcatc tattattaca caataaatca gcaataatga attcattttt agaaggagtc
     3001 aggcaaaggg cccgccagtt gaataaatgc atagtgttgc ccgaaggaga ggaggaacgc
     3061 actttaattg cagcgaatga agttttaaaa gaaaatttgg ctcgaattat actcatgggc
     3121 tatcctgagg taattcgtca gaaatcgaaa gacttaaatc ttgagcatat atcaaaggcc
     3181 gagattgttt ttccggatgc gtatgaaaag aggcagtatt acgctgacct tttgtatgaa
     3241 atccggaaaa ataagggaat gacacccgaa caggcatatc gtttagctgc tgacccatta
     3301 tatttggcaa gtttatttat aaaagatggg aaagccgacg gtgaagttgc gggtgccatc
     3361 aatactacag gcaatgttct gcggcctgcc tttcagattg ttaaaactct cccaggtgtt
     3421 agtgtagtat cgggagctta tataatgata tttaaagaca gccattgggg tgaaaatggg
     3481 atgatgattt ttgccgattg tgccgtaaat cctgatccta cagagtgcca gttggccgag
     3541 attgccgtca tgtcggctcg cactgcaaga atcctggctg gcatcgaacc gcgggtagcc
     3601 atgttgagtt ttagtacgaa aggaagtgcc acgcatactc tcatcgataa agtctctcat
     3661 gcaaccgaga ttgctaagga aattgacccg tccttgatga tcgatggaga gcttcaggcc
     3721 gatgctgcaa ttgtagaaga agtagcgaaa aggaaatgtc ccaatagtcc tgtgggcggg
     3781 aaagccaatg ttctgatatt ccccgatctt caatcgggaa atatctcata caaattggta
     3841 caacgactcg gcggggctga tgcaattggc ccggttttgc aaggaatggc cgctccagtg
     3901 aatgaccttt cccgaggttg cagctctcaa gatattacaa atctcatcgc tattactgtt
     3961 attcaggcag gtggtttata gcctttacaa agagatgcaa tggctatgaa tttatcctat
     4021 aaaattcttc caaaatcaca atttatatga atctttcgtt tcaatgcaac tggtattttc
     4081 tctgaactgc ataatacgac gaactctcat tcttttcttt ctcgatcgga attattttta
     4141 tagaaatgat tcggttaata caaaatgcca acgtccaaat ttgtttcgaa aaatttgatt
     4201 tttgattttt tagcaatata accaataaat ttgtaatttt gctacgtatt tcttttttta
     4261 tctatgtggc agaagtattg gccgataagc acattatatc acaataacgg gaaaggtttg
     4321 tattcctgtt ggatgctctg aaaaatcatc aattacgatt gtcagggtct gctatcattg
     4381 attccttttc aatttacggt tttttttaca agactttgtt cgacctttct attcttgaag
     4441 cagacttatc tcattttccg aattattata tcattcaatt tgtaaatatt ccttagtttt
     4501 tctcgatcta ttctcgataa attccaatga aagctttgat attcgatatt cgacggtttt
     4561 gtgtcaatga tgggccgggc attcgtacga cggttttttt caaaggatgc ccattgcgat
     4621 gtttatggtg tcataatccc gaaagccagc ttaacaagcc ggagccattt ttacggatgg
     4681 ataaactgga aggtaaattc ttcgagcatg aagaacaaat cggttatgaa atcgatctgg
     4741 atgaattatt agaaatttta tttagagata gggcttttat ggaagaaagt ggaggtgggg
     4801 ttactctgag tggaggagaa cctttgtggc aatggaagtt ttcagcagcc ttagcacaga
     4861 aattgagcga gtcaggtatt cacgtagcag tagatacttg tggttatgtt cagttacggg
     4921 ttttcgaagc catattgcct tatacccatc tttttctcta tgatttaaaa ggctatgata
     4981 accacaggca caaagaaaat actggagcaa gcaattctct catcctcgaa aatcttgaat
     5041 atctcattca ccagagagcc aatatcatct tgcgatatcc tgtaattcct ggttataccg
     5101 atagcgaaga cgatgtgaga ggaatactat cgatcatgaa gcgaaactct gatatactga
     5161 gagaaataca tcttttgcct tatcacaaat taggacagaa taaataccga ggcttacaag
     5221 cctcgaatcc catgcctgag aatattcccg atgcagatga aagtgttggt catattgaat
     5281 cgatctttaa atcaaatcat tttattgtaa aaattggagg ataaaccatg aacacaaaaa
     5341 taacacagcg gatcgagaaa ttaagagaaa aaggtcttag agccgtaaac agaatctcag
     5401 ccgaacgggc tttattgatc acccagtttt atcgaacggc tgagccttac agagtatcgg
     5461 ttcctgtagc ccgggctatg gctttcgatt atattttaag tcacaagaag atttatattg
     5521 atgatggaga attaatcgtg ggtgaacggg gtccagaacc aaaggctgta cctacctatc
     5581 ccgaagtaag cttgcattct ctcgaagacc ttcgaattct gaataccagg ccgaaggtat
     5641 catttcgagt ggatgaggat acttttgcag cttatcgaga tgtaatcatt cctttttgga
     5701 aaggaagaag caacagagaa aggataatgg gattaatgga tgaagactgg ctcagaagtt
     5761 atgaagctgg tatttttacc gaattccagg aacaaagagc acctgggcat accgttggag
     5821 gttcgaaaat tttcaatagg ggcatgctcg atctcattga agacatccac cgagtgcagt
     5881 tgaaactcga tttatatgaa gattacctgg cttttgaaaa aaatgaagag ttgaaggcaa
     5941 tggaaatagc cgcaagggct ctgattcatt ttgctaatcg tcatgccgat ttactcgaag
     6001 aattatcaaa acaagaagaa gatttacagc gaaaatcaga gttaaaggaa ttggctcaaa
     6061 ttagccgacg cgttcctgcc tttgctcctt ctacctttca tgaagccctt caacattact
     6121 ggtacattca tctcggcgtc atcacagaag taaacccatg ggacagtttc aatccaggtc
     6181 gcctcgatca acatctgttt cctttttggc aaaaagatag aaacgaagga agattaagcg
     6241 atgagaaagc atgtgaacta cttgaagcat tttggataaa attcaataat cacccagcac
     6301 ctcccaaggt tggggttacg gctcaagaga gcaacactta taccgatttt gctcttatca
     6361 acgtcggtgg tgtgaaggtc gatgggagcg atgcagtgaa tcctctcagc tatttgattc
     6421 tggatgtagt tgaagaaatg cgaattttac agccaagcag tatggtccag atcagcagga
     6481 aaaatcccga ccagttgctt cacagggcat ttcaaattat tcagaccggt tttgggcagc
     6541 ccagtctttt caacaccgat gccattattg ctgagttagt ccgtcagggg aaaagcatag
     6601 aagatgcccg caatggaggt gcaagcggat gtgtggaagc cggtgccttc ggaacggagg
     6661 cctactggct gacaggctat ttgaatcttc caaagattct cgaaattaca ctcaacaatg
     6721 gtttcgatcc tcgaaccaag gttcagcttg gcccaaacac cggaactgct gaatcattcg
     6781 agaattacaa ccagcttctg aatgcctttc aaacccaact caatcatttt atccagatta
     6841 aaataaaagg caataatatc atcgaaaagc tttttgctca ttatcttccc gtaccttttc
     6901 tttctcttat tatcgatgat tgtatagaaa acgggatgga ttacaatgcc ggaggagcac
     6961 gctacaacac aagttatatt cagggagttg ggataggcag tcttaccgat agtctcacag
     7021 ccatcaaata tcatgtcttc gataagaaaa ctctttcgat gaataatttg ataaaggttt
     7081 tggaaaacga tttcgaagat gatctgtctc ttcgtcactt attggtttat caaaccccaa
     7141 aatatggaaa tgatgacgat tacgctgaca ttgaggctca aagggtgttc gaaatgtttt
     7201 ttgaggctgt agatggaagg cctacagcaa gaggaggcaa attcaggatc aatttacttc
     7261 ctacaacttc tcatatttat tttggaagtg ttattggagc tctacccgat ggacgtaggg
     7321 ctggccagcc tctttcggag ggtatttcgc ccgtgcaggg agccgatcat catggtccaa
     7381 ccgccgttat ccgttcctgt gccaaattag accacctgcg aaccggcggt actctcttga
     7441 atcaaaaatt tactccctcc ttcttcaaaa accatgaagc catacaaaaa ctaactgccc
     7501 tagtaagaac atatttccga ttggatggtc atcatataca atttaatgtg gtgaatgccg
     7561 aaattttgag ggatgctcag cgtgaacccg aaaaataccg cgatttgata gtgcgcgtag
     7621 ccggttattc cgattatttc aacgacctga ctcccgaact ccaggaagaa atcatcagaa
     7681 gaactgaaca cgaaaccatg taattcattt tgtcgtttta attattgacc tgatgacagc
     7741 tttttcgggg ctttcgtatt ttctttattt ccaaactaat tttgcatcat ggaattcgaa
     7801 gagataaaaa ccaaagccga tgcaaagtat ctggcacttg tcaaggtaat caaaccttac
     7861 ctgcacagaa acgacactgc agtattgcgc aaagcatacg aaacctcact cgtgcatctg
     7921 ggagaaataa tcaacgaagc acgtcaaccc ttggttcttc agcaactcga tattgcactc
     7981 attcttgccc atcgaatggg aatggggccg caaaccgtgc tacctgcgtt tgtggttgaa
     8041 atgttgagat ttgggaaaat agaacccact cagatttctt cgacctttgg cgaagttacc
     8101 ctgaaaatta ctcagggatt gttatccatt gagaagctta acctatcgaa agtttccacc
     8161 ggttcggaaa atttccgtga acttctcatc agcctttcca atgatataag aggcatcttt
     8221 tatctaatgg ccagaaacct ctatgatttg aaccattatt cttctttgcc tacggcatct
     8281 caacaattga tcatacaatt ggcgagagat gtttttatac cctttgcaca tcggcttgga
     8341 ttgtataata tcaagactca gttagagaat gatgtgatgc gatattgcga gccagaaatc
     8401 tatagagata ttgcacaaaa aatggaagaa cttcgtccga gccaacaatc cttttttacc
     8461 gattttatta ttcccattcg cgaagcttta tcggaagcag gaatcgagtg tgaattcaaa
     8521 tggagattca agacaattcc cagtatatat cagaaaataa aaaagcaaaa tgttgatatc
     8581 caacatgttt acgatctctt tgccgttcgc atcatactta caggccaact cagcgatgaa
     8641 atagccgagt gctggagagt ttatagcata gtaagcaata tttatccacc cgatacttca
     8701 cgcctgcgtg attggataac acgcgcccga ccgagtggct acgagagtct tcatactact
     8761 gtaatgactc ccgaaggaaa gtttgtggaa gtacagattc gcacccgtcg tatggacgaa
     8821 atcgctgaaa aaggtgatgc tgcccactgg cattataagg aagcaggtag aaaatcggag
     8881 atagatacct ggttacggga aattcgtgaa gttctggaat cttatcctgg agagggagcc
     8941 gatcccttac atactcatca accattgccg tcttctactc atatttttgt gcttactcct
     9001 gcaggtgatg taaaagaatt gcctcagggt gcaagtgtcc tcgattttgc cttcgaagta
     9061 cattcgagcc ttggagccag ttgtacagga gcccgggtaa atggcaaagt agttccctta
     9121 aaacatcgat tgtccaatgg cgataaaata gaaattctaa gctcaaaaaa tcaatttcct
     9181 aagatagatt ggctgcaata ttgtgttaca tcgaaagcca gaaacaagat cagaagagca
     9241 ttgagcgagg acaagcttgc cgaagcagaa acagggaaaa gcgcactgct tcggcactgc
     9301 agaaaatgga aggtaaatta taacgatgag atggttgaaa agctgcttaa gcattttaaa
     9361 atgaaaacgg ctctcgaact ctatgcttca ttttcacatt ctgatatcga ctgggcagaa
     9421 gtcaaaagaa ttcttaccga aaatactttg tcagaaatcc atattgttcc accggtgaaa
     9481 aggaagaagc ccgtaaagga agagcctgcc cccaatgcca tcatcatagg agatgaatcg
     9541 aaaaatattc attattcgct ggcacgttgt tgttcgccta tttatggaga cgatgtattt
     9601 ggttttatta cggtaaatcg cggtattacc attcatcgca aaaattgccc taatgccctg
     9661 accttgtttt cgaaacatgc ttacagggta gttgaggctc gctggttcga aaaagctggt
     9721 gaaggccctt atcaggcttt gcttcgtatt acgggcgacg atcgaatcgg ccttctggcc
     9781 gatatcaccc atgttctgac caatgaactg aatgtcaata tcctctctgt aaacctcgat
     9841 tcgaaagatg gattcttcga tggaaagatg aaagtgcagg tgacaaatgt tgctcatctc
     9901 gattcggtta ttcatcggct tatgaaggta aaaagtgtgg ctagggtgaa acgcttctat
     9961 gattaataat tttaaaaggc ttaatttttt aattttgcgc cttcaaacac ttatattgta
    10021 tgtgggctat gaaaaaaata aacctggctg tatgtatttt gtgcttaggt gcattaagta
    10081 tttgttcttc ttcatgtaaa caaaacccta aggcaaactc atcgggtgaa gtagagaaaa
    10141 cgaaccaaca aaaatctccc gaattcaaat acgacgacct tgaccgtatt gtgcaaagaa
    10201 aggatttttc ctataaccgt gatggttctg tgcgttataa gaaccagtac gattataagt
    10261 acgacgatcg aggcaaccga atcgaagaaa ttacgcaatc gtgggatttg agtggtaaac
    10321 gcgtggtcta taccattgcc cgttttcact tcaatgactt gaatctgaaa attgaggcca
    10381 tttttcagtc attcgatccc gaaggccgtg aaatcttatg ggtgaaaaat gtatatgatt
    10441 atgatgataa tggatatgaa attcgtgacc aacaattcga taaacaaggg cggcctcttt
    10501 cattagtgac ccgaaaacgc gacgaaaagg gcagaattct cgaagagact actactattt
    10561 ataatcctga tggaagcata aaggaacata aaggtgccca gtacgacatc aatggtagaa
    10621 ttttgaaaac ttggtaacat aaattttttt acacctgttc ctaaataact gcaaaaatca
    10681 aacgaaatga aatattcaac atcaatgaat gaattaattg acttttcttg tggttatgtt
    10741 gtatttcagg aaatgaaaat attaacttca ttaaattacc tatcataaaa ataaatttat
    10801 gattctattt ttgattcatt ttgttgaatt acctgctaat ctggtatgag aagcatggca
    10861 tgttccttca ttatcattcg aacaacatgg tcgtttgaat tcatgatgat tgatgcttga
    10921 aatggcatga aagatttttt ccgataacaa aataatttca tgaataaaaa acttttgatt
    10981 tttgttttcc tcacttttca gattattgtt cttcgggcaa attcccagat cgagccagtg
    11041 aagcccgaaa atttgtcact tctcaaacga atcgaatttt ggtacgaaaa aaatatgaat
    11101 tatggaagta ttacgatttt aatggctatc gaaagttcat tcattccctt tccatctgag
    11161 gtaattattc cgccggcagc gtatattgcc agcaagcccg gaagtgagct caatataata
    11221 ttaatagtca tttttggtac tctgggagcc ctcttggggg ctttcgtcaa ttatttcttg
    11281 gctttatggt taggaaggcc tttaatgtat aaattagccg acagcagatt agggaggatg
    11341 cttttattaa gtagtgcaaa aattcagaag gcggagaatt atttcaacca gcatggaaat
    11401 atttctacct tcattggtcg attgattcca ggcataaggc aattgatttc attgcccgca
    11461 ggcctggccc gaatgaacct tctgagcttc actttattca ctgcacttgg agctactaca
    11521 tggaatatca tccttgccct actgggctat attgctcacg gtcaggccga tttaatcgaa
    11581 aaatacaaca aagagctcag ttacattatt ttagcaatcg taggattggt cgccttattc
    11641 tatatcctca aatatgttag aaaacgaatg aaatctacca aacaattacc tttataaagg
    11701 cattcaactc gagcacgatt ttttcgaata ttcaccttcg ccgaaagact tgaaaaatga
    11761 acatcctcca tcatactatt gttttgctat ctttgctcga tttatccgaa tcttttcgcg
    11821 attgcggctt ttcgaacttt cgttatgatc cagaaaaaca acttacgttt tagtattttt
    11881 atcgttttta taatagactt aattcttctt tttagctatt cttcatcggc tcagcaaaaa
    11941 aggaggattc tgataaaaaa ggcggatatt cttatcagtg aagaatggca tgatactacc
    12001 gtcaacctct tgcgtggcca tgtggtctta gaacacgaag gggccttgat gtactgcgat
    12061 tcggctttta tgtatgaaca actcaatagc ttcgatgcct ttgggaatat ccatatcgat
    12121 gtaagcgaca ctctggatct ttttggtgat gttctatttt acgatggtaa tacccgaatt
    12181 gccgaagttc actataatgt ccgtttaatt gacaagacaa ctaccttata taccgaacat
    12241 ttgttttatg atcgcaatct cgagataggg cattatcccg atagcggaag aattatcgat
    12301 aaggacaata tacttaccag tcgcaatgga tattatttta gtcgtccacg tgaatatcgc
    12361 tttagagaaa atgtagttct ccgaaatccc gattatgtgc tggttacaga taccttgaac
    12421 tatgatacta aaacaaggat tgcttatgtg aaaggacctt ctaccattac ggggaaagaa
    12481 agatacgttt atgccgagga tggttggtac gacacccaaa aggagatggt aagcctgaaa
    12541 acgcatgttt tcatgagaaa taaggatcgc accctgaaag ccgatagtgt tttctatgat
    12601 gacaaaattt cgtttggaag agcctttcga aatattacgg gccacgatac cacttacaga
    12661 gttattttga atggtgattt tgcagaatat tccgattcat taggttacgt tttttttact
    12721 cgaagagcca gagtacttta ttacgaaaag aaggataccg atactattta tatccatgca
    12781 gataccttgc gtttgacctt cgacactgcc caacaggcca aattttttta tgccttcaac
    12841 catgcaaaat tttaccgcca cgatattcag ggaatgagcg attcgatgct atataactat
    12901 ccggattcta tgtttgtctt atacaaaatt ccggttcttt ggcaagattc caatcagctt
    12961 actgccgaca gcataaaaat ttgggtagcc aaccagaagg tcgacagtat gaccatgtat
    13021 aatacggcat ttatcataca acgtgatgat tctggcacct ttaatcaaat gcgaggccgg
    13081 gatatgttcg cttatttcgt cgatggcgaa atgcgaaaag tattcgtgaa aggcaattca
    13141 gaaacagttt attatatacg cgaagaagat ggaagcctta ttggcattaa tctggctgtt
    13201 tcgagcaata tggtcttgct tatcgaagat aatgaactta cagatatcat ttatttgata
    13261 aaacctgaag gtggcacgta tcccgagaaa cagtttccgg ccgatagatt gaaactgaaa
    13321 ggctttgaat ggcacgaaca acgtcgcccc cgaaaacctg atgatatctt catctggtga
    13381 gtttcaaaaa actgaaaatt taaggtaacg actcgggttt tcgcgaatat cgcgaatcaa
    13441 tagctccaat tccttggtag agcgcgaaag attgtcgtaa aggctgtcac tgcttagcaa
    13501 taatcctgcc gatccttctc ctcgttcgat ctttattagt atttcggaaa gcaactccag
    13561 attataatcg agttttttga aggtttgctc aatgtgtact tgtgccagcg tatcgcttat
    13621 ggtgtgaata ttcgagatga cggctgtaag ctgttcgttg ttgctttcga gatttgccga
    13681 aatagattct atgttttgaa taataaaaga aacccttgat ttattggaaa ggattaaagt
    13741 atcgagtgta gcggtaatct gcttgatgtt tcgtaacgaa aattgaatgc tttgaagact
    13801 tttcgaaata ttttccctgt tctcatgatt caatgaaact tgtagaactt tcattgttga
    13861 atcaattgaa tttaaggcca gttcggcttt ttgcatcacg ggtgtcaatt gcctgttcaa
    13921 tccttccaat aaacccgcat ctatagcacc agcaagcgta tcacctggct gagcacgatg
    13981 tggagaatct ccaagaatga tttcaatggc ttttgcaccc attagatcac gattgataat
    14041 tgtagccact gaattattgg gtatgggaaa ttgatttacg atgttgagtt ttactactac
    14101 cgaaccattc atatttttat gaaaaaacac gtcggaaacc ttacctacct gcaaccctcg
    14161 aatgaagacc ggtgatgttg gtgcaagccc aacagcctga tcataaacta catagtaaaa
    14221 ttttttatta tgaaatattc catttccttt tagaaaagaa tatccccaaa taaatataat
    14281 gatgacccca ataaaagtta atcccgtttt aaattctttc gatattttca agacagggta
    14341 atggttttaa ttttaattct ggtgcgaaat tacaacaaat tcgatcccga ataatttcat
    14401 taactttttc atcagggatt caatcgttga tctcctcgaa atggaaccac gaaagcatct
    14461 ttgaaaccct gttttcgtaa tgagtcacgg tattggattg cgtcatcttc gttgaaaaaa
    14521 tggcccgaag tgtatttgta gtagccttta tgtttatatt cccagatatc ttttaatccc
    14581 ttgaagaagg aatggttttt actaagaggt tgtgtagagg cggttatttg tacccggtaa
    14641 attatgggcg aacttgtgga tgcgattgtt tcggttggac tggaagatat agcttgtgca
    14701 gtattttttt gctgagcgaa gctcgaagtt tgcaaggttt tgttctgctg gtcggccgta
    14761 gatgaaagaa gctctgaatc gaccgtaata tcaggtttaa cgggggccat gcttgagatg
    14821 gcaggaatat tttcgacttc cattttgtaa gcgttgaaag cgctggcaag cgacgtggcc
    14881 aatttttcct gtccggcttt cgatttgagg aaattttctt cgttttcgtt gctgatgaat
    14941 cctaattcaa ccagcactga gggcatggta gtatgataaa gcaccaaaaa accagcttct
    15001 ttcaccccac gatcgaaacg tgaagctact gtattgagct gattttgaat gttcgaggct
    15061 aataaaaggc tttgttgcct gtagagattc tggtacatcg aaaaaatgat ttcggtttct
    15121 ggggtattag ggtcgaaacc atcatatcga gtttggtaat tttcttcgta caatacggca
    15181 gcgttttcga gtttagccac ctcgaggttg gcctggcttc gatgaaggcc catcacaaaa
    15241 gtctcagccc cgtagtggat acgcgatttg ctggcattgc agtggatgct gataaacaaa
    15301 tcagccttat ttttattggc aatatcggcc cgtttgtata attctacaaa actatcgtcg
    15361 cttcttgtgt aaataacttt cgtctcggga tgatttttgg taatatattg ccccagtttg
    15421 agagctaccg acagggtaat gttcttttcg gcgcttgtct ttcctaaagc gccggggtct
    15481 ttaccgccat gcccggcatc aatgaccaca gtacgaattt tcccatcttt cgataaggct
    15541 aaaacttcat cattcagaga aataatcatg cttgcaacca tcaccagaca aaacaagccc
    15601 ctgaaagttg aaattaaatt tttacttttg gaggcccaat attttttagg caatttgttt
    15661 tttttcacaa tcgttttatt caaaatacac tttcagcctt tagattccaa aaatacactt
    15721 actcatccgc tttttaatca caaaagattg aaagtttttc caaaagatat taacaatttg
    15781 gtgcatatcc tcgtcggcac acactttttg ggagatttgt catctacttt ttggaaaaat
    15841 attttcattt ttattgtatt tattcttatc ttagggaact ccctttcacc ccaaaatatt
    15901 gtcagggata ccttaaaaaa cgattcattg attttattcg atactaagaa cttcattagc
    15961 cctattgggg atactttgcc agagagagac agcctgatca tagacaccaa tcaggtcgtt
    16021 ccgaagccca aatcgtcgaa aaatgtcctc gaagatattg ttaattacaa atgcaatgat
    16081 agtatccgat tcagagtgaa ggagcaaaag gtttatctat ataaggatgt agagattgcc
    16141 tatcaaaaga tgggacttac tgccgattat gtagaggtga attttagact caacgagatt
    16201 tttgcctcgg gatggcccga ttctactgga aagataaggg ggactcccgt ttttaccgaa
    16261 ggagaccaaa gtttcaaggc aagcaaaata cgattcaact tcaagaccaa taaaggattg
    16321 atcgagaatg taataactca agatggggag ggttttcttc atggtaccat cgtaaaacga
    16381 atgcccgacg atgtgattta tcaaaagggt ggaatgtaca caacctgtaa tctcgatcat
    16441 ccgcattttg cctttcagtt caataagggg aaagtgatac cgggagataa aatcgttacc
    16501 ggccctgctt atttaaccat agaagacgtt cctacaccac tgttcgttcc ttttggtttg
    16561 tttcccaata aaaaaggcca acgttcgggt gtaatcattc ccagctatgg agaatcggcc
    16621 aataggggat tttatctgga aagagggggt tattattggg ccataaacga ttatatggat
    16681 tttactctca caggcgatat ttatactcgt ggctcatgga aaataaatcc acaattacgc
    16741 tatgccaaac gatataagta tagtggtaat atcgattttg cgagagcatg caatatcagt
    16801 ggagaacccg gtgacccggg ctatttcaat agtcgcgatt ttcaatttcg ctggtctcat
    16861 cgccaggatc ccaaagctaa ccccaacagt aatttttcag ctaacgtgaa tatcgtcagc
    16921 aataaataca atcatttcaa tcccaatcaa atgaacgatt atttttcgaa caccttccag
    16981 tcgagtatat cttatcaaac aaaaattggg cagaattata accttaccat gaatgccaat
    17041 cattctcaaa atactctgaa taaaacagtg aatatcagtt tgccacaaat cacactaacg
    17101 ggtaaccgct tcaatccatt caaaaaaaag aatcgaattg ggaaagtaaa gtggtatgaa
    17161 aatattacca tgtcgtattc tatgaatgcg gaaaaccgta tcaatactta cgattcgttg
    17221 ctttttaagc acgagacatt gaaaaatatg caaaatggca ttaaacatag tatacccata
    17281 agcagttcta taaaggttct caaatatttc aacttgacta attcaatcaa ttataccgaa
    17341 agatggtatt tcagaaccat tcacaaagcc tgggtcgata ctaccgactg gagccacgac
    17401 acggttgctt caggatatgt tcgtaccgat acggcctatg gattcaagtg tgctcgtgat
    17461 ttcactttta gcagcagttt gaatacccgt atttatggta tgtttacttt catgaaggga
    17521 cctatcgttg cagtgcgtca tgtggtgagc cccagtgtga gtttttcgta caatcccgat
    17581 tttagcagtt cattctgggg ttattacaaa ccttaccaga aaaatgcgaa aggtactatc
    17641 gataaatatt ccatatttga gggcggaata tacggaagcc ctataggatc aaaggcaggc
    17701 cgaatttcat tttcaattag caataacctc gaaatgaaag ttagaaaccg taaagatacc
    17761 cttacggggg taaaaaagat acccttgatc gataatctta ccatcagtac ttcttatgat
    17821 tttatgcgag atagcctgcg gtggtcgaaa gtgcagatga gcggccgcag tacacttttt
    17881 aaaaacatta ccttgaatta ttccagttcg tgggaccctt atattctgga tagtaccgga
    17941 aacaaaaatc ttaaccaata cgaatggaaa gttaatcgaa gacccttacg actggataat
    18001 acaaactgga atttatcatt aaactggaat ttttcttcca aatcgaaggg tagtggagga
    18061 aaacctccgt taaatcccaa tgaccccgaa tatcaggatt atttgctcaa tccagatagt
    18121 tatatcgatt tcgacattcc atggaaactc aatttggctt atacgctcaa ttatacagct
    18181 aaccacaagt atccttatta caaggaagaa gttatcagaa aaattgttca aaccttgaat
    18241 gtttcaggtg atatcagcct tgctccacgc tggaaaatag gtttttcaac tggatacgat
    18301 ttcgaaacta acaagatatc gcaagcttca ctcaatattt accgcgacct ccattgttgg
    18361 gaaatgcggt tcaactggat tcccataggc tatctgaaaa gctggaattt ccaaattaat
    18421 gtcaaatcgc caatgcttca ggacttaaaa ctcacaaaga agaaagactt ccgcgacagg
    18481 tgatgcatag aatctataat cgagaataga atcctatttc ttgcccaatt ttttgagaaa
    18541 cattgtaatt ccattggcaa aagtatgcca gggcgataag atccatacta ttctggaaat
    18601 tcgagtaaaa ccgaaatctc tctttatacc acatgtaatg gatgacccta tccgaattat
    18661 gaaaaaaacc ttcggatggt gaaaccgtag tattgacccc ctcttatgat tgtgacagcc
    18721 atagcctgct gatctctcca attcttcacg cagttttttg tcgaagtgat g
//
LOCUS       DAOORU010000025        18292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_3709_length_18292_cov_5.069748, whole genome shotgun sequence.
ACCESSION   DAOORU010000025 DAOORU010000000
VERSION     DAOORU010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18292
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3709_length_18292_cov_5.069748"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1556)
                     /locus_tag="PLF20_03035"
     CDS             complement(12..1556)
                     /locus_tag="PLF20_03035"
                     /inference="COORDINATES: protein motif:HMM:NF019187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80016.1"
                     /translation="MQNKGIVKFFAILFVLVCLFQLSFTFVSKHIESKGKSYATSPKA
                     QALAKQIAKGDKFRELLVYDSLVKAREKFFFDSIADQVVYNIGVRKYTYKEVKERELN
                     LGLDLKGGMNVTMEVSVPDILKALSNYSTDTTFNEALRLAREKQKNSNADFVDLFYES
                     FKELDPNGSLAAIFATVELKDKVKFNSSNEEVLQVLREETKAAVDRTFNILRTRIDRF
                     GVAQPNIQKLQTTGRILIELPGIKDPDRVRKLLQGSARLEFWPTYNFDEVYQFFDEAD
                     KRLMAIYKEEGLITPEDTIASAKNKEGGITDSLQTTEADTGLLAKLADSARLQKSKQT
                     SFEEYAKEHPLYAYLIPSYYPDENNQFHPAKTARVGSAAIKDTARVNAMLKRVKDVFP
                     SNMRLHWTVKPDPQRPGILDLVAIKVTRPDGQAPLGGEVVIDARQDVNPTTGGVEVSM
                     TMNREGAAEWKRITAENIGRQVAIVLDGYVYSYPVVQTEISGGRSAISGGGMTEMADL
                     PPEISV"
     gene            complement(1672..3009)
                     /locus_tag="PLF20_03040"
     CDS             complement(1672..3009)
                     /locus_tag="PLF20_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX80017.1"
                     /translation="MSKISLIIEREYITRVKKKTFIIMTILGPILIAALWIVPIYVTT
                     LSQGKKTVEILDETGLFFDQFQSDEHFRFVPIFTDLETAKKGLIASGDDALVYIPRTE
                     LNVPVTAILYSTSQTGIDIKTVVKENLRKQVEALKLKAKNIDPEVLTSIKTSIIINSI
                     KIGKDGSEVKSSEEISIALGLLSGIIIYMLIFMFSSQVMRGVIEEKANRIVEVIVTSV
                     KPFQLMMGKIIGVGLVGITQFVLWIVFTGVIIFGFQITHPAIQQNVSQIENFSSTSIV
                     SSGQSVDLNEDMVLESNIITQALEGIRAINIPLLLFAFILFFLGGYLLYSAMFAAIAA
                     AVDNEADTQQFVLPITIPLLITIILSGFIINNPQSQLAVWLSIIPFTSPVSMMMRIPF
                     GVPVWQLILSILLLVATFIIATWIAGKIYHTGILLYGKKITWADLIKWISMKL"
     gene            complement(3019..3957)
                     /locus_tag="PLF20_03045"
     CDS             complement(3019..3957)
                     /locus_tag="PLF20_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX80018.1"
                     /translation="MDLIRTEEVTKRYGNYVALDNINISVPKGSIFGLLGPNGAGKTT
                     LIRIINQIIAPDKGKVFFNGQVLQPNHTDKIGYLPEERGLYKKMKASEQAIYLARLKG
                     VSQHEAVSRLKYWFRKFEILSWWDRKVEELSKGMQQKIQFIVTILHQPELLIFDEPFS
                     GFDPINVDLIKNEILKLRDEGASIIFSTHSMESVEQLCDNIALIHQSKVVLSGAVNEI
                     KSRFSQNLFEVESYKADLSTITPNLPQGIHYQKTEPNSSLYKHYFKLDASLAPNDLLA
                     SLLPYMQITGFRELLPSMHDIFVSTIQNGSLPANPS"
     gene            complement(3972..5138)
                     /gene="dnaJ"
                     /locus_tag="PLF20_03050"
     CDS             complement(3972..5138)
                     /gene="dnaJ"
                     /locus_tag="PLF20_03050"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOX80019.1"
                     /translation="MAKRDYYEVLEVSRNATGEEIKKAYRQKAMKFHPDRNPGDKDAE
                     EKFKEAAEAYEVLSDPEKRSRYDQFGHEGVGGAAGGGFSGHGMTMEDIFSNFGDIFES
                     FGGFGGFGGFGGSSRTRSRKTASHGTNLRVKVSLTLEEIAKGAHKKLKVNKYIKCKAC
                     NGTGARAGSSPHVCPTCRGTGQIIQTTNTFLGRMQTTSTCPQCGGEGRIITDKCPECY
                     GDGIVKGEEIVEVDIPAGVTDGMTLSLSGKGNAGPKGGLSGDLLIVIEELPHPDFQRQ
                     GNHLLYELYISIPDAILGTTVEVPTLEGKARIKIEPGTQAGKILRLKGKGLPEVNSYK
                     KGDLLVTLNIWIPKNLTSSEREMIEKLRLSENFKPHPDKKEKTFFARMREFFNS"
     gene            complement(5169..5846)
                     /locus_tag="PLF20_03055"
     CDS             complement(5169..5846)
                     /locus_tag="PLF20_03055"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOX80020.1"
                     /translation="MQPQDLKKPEEKNSMNNDHLDENEEQTNNDLHEDRSMQELNDKV
                     TRGKRRNKHGKTSTNESEVIELEAKMKELEQANEKSEEEIAEWKDKFLRLFSEFDNFK
                     KRNARERLELIKSANSSLIVKLLPVLDDFERALDSLNQNDDEVLKPYHDGIELIYNKF
                     YQILEKEGLKPIGNVGETFDTDYHEALTMIPAPNEEMKGKVVEVIEKGYMLHDKVIRF
                     AKVIVGS"
     gene            complement(6002..7327)
                     /gene="murA"
                     /locus_tag="PLF20_03060"
     CDS             complement(6002..7327)
                     /gene="murA"
                     /locus_tag="PLF20_03060"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOX80021.1"
                     /translation="MDYNNKNRQVYRIKGNKTLSGEITPQGAKNEALQVICATLLTPE
                     EVIIHNVPHILDVLRLIELLEGLGMKAKRIGEHSWSFQADDIHPEYMQSREYHQKARA
                     LRGSMMVVGPVLARFGKAYSPRPGGDKIGRRRVDTHFNGLQKLGAKIGENAATHIPFL
                     EASKLKGTYILLDEASVTGTANIVMAATLAEGTTTLFNAACEPYVVQLCKMLNRMGAR
                     ISGIGSNLLTIEGVEHLGSTTHTLMPDMIEVGSFIGLAAMTQSQLIIKNTNYRSLGII
                     PEIFRRLGVETFPDNNDIIVPTKEHYEIESFCDGSIMSISDAPWPGFTPDLISIALVV
                     STQAKGSVLIHQKMFESRLFFVDSLIEMGARIILCDPHRATVIGLDWKYRLRGICMSS
                     PDIRAGVALLIAALSAKGESIIENIEQIDRGYENLDARLQVLGADIERI"
     gene            complement(7332..7955)
                     /locus_tag="PLF20_03065"
     CDS             complement(7332..7955)
                     /locus_tag="PLF20_03065"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HOX80022.1"
                     /translation="MEYNTTRGPLKMAEYGRNIQKMVEYIIQIPDREERTRAAQALVK
                     CMEQITPEQKDYTDYKRKLWDHLHIISDFRLDVDAPYPLPSKEQVELKPQPIPYSDNK
                     IKYRHYGRNVQKMIDYVSTLDTDHPDRKAMIRVIGNSMKMAYLTWNRDNVADDVILEQ
                     MKELSDGKIDTEGIKLAPTNELLQIIRQQQKLQPVAKPGNKKKRKHK"
     gene            complement(8093..10384)
                     /locus_tag="PLF20_03070"
     CDS             complement(8093..10384)
                     /locus_tag="PLF20_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOX80023.1"
                     /translation="MEKDILSHLNEAQREAVRCIEGPVMVIAGAGSGKTRVLTYRIAY
                     MIQQGIDPFHILALTFTNKAAREMKERMLQLVGKSEAVNVWMGTFHSIFARILRVEGH
                     LIGYPAAFAIYDAEDSKRLMKSIIDEMHLDLKIYSPSWVLTRISAAKSRLISVQEYLD
                     STEIQTADREANRPETGILYKIYQDRLFRSQAMDFDDLLFNMNRILQTFPEILFKYQN
                     RFRNILVDEYQDTNFSQYIIIKQLAARFENICVVGDDAQSIYSFRGANIQNIFNFKND
                     YPDYKVFKLEQNYRSTKIIVEAANKVIANNREQIYKKVWTDNEAGEKIKIIRASSDNE
                     EGQLIADTIFELKMNRQLESDAFAVLYRTNAQSRAIEEALRRLNIPYRIYGGLSFYKR
                     KEIKDLLAYFRLVVNPNDEDALRRVINYPARGIGEGTMDKAMVCSIERNVPLWEVISN
                     PLEYGLQLRSNIVEKMSNFVAMIKKFHYQIKTHNAYELARQIAHASGILKELDDDKSP
                     EGVARLDNIEELLSAINDFSQNDEVIPNIDQEDQTGIRSLDLFLQQIALLTDADEKDD
                     GVPKVSFMTIHSAKGLEFPYVFVAGLEENLFPNIQALGTRIELEEERRLFYVAITRAQ
                     KKLFLSHAQTRYHHGSLNYCEPSRFLDEIEEEFIESGAKKVSWNPPFASVKSPKTIPT
                     KQPDFSKLKKISHEDVNSPLNPDTEELIRCLQTGMQVRHERFGQGKVISLEGEGANKK
                     ATVFFPGIGQKQLLLKFAKLKII"
     gene            complement(10697..12043)
                     /locus_tag="PLF20_03075"
     CDS             complement(10697..12043)
                     /locus_tag="PLF20_03075"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX80024.1"
                     /translation="MKHSKFSMESNINTGVLKNIFRSLHYRNFRLFFIGQSISLIGTW
                     IQRIALPWLVYDLTKSAFLLGLVGFAGQIPMFFIAPFAGVITDRLNRYHLLVATQVFA
                     MLQAFILAFLILFGVINIWLILVLNILLGCINAFDTPVRQSFFVEIIDNKDDLPNAIA
                     LNSSMVNAARLLGPSIAGVLIAFTGEGMCFLINALSYIPVVTSLLMMRIPLKTHEKNS
                     SHVLIELKEGFFYTFGHKVLKHIILLLALVSLMGMPYTVLMPVFAKEILHGGSYTFGF
                     LMGASGLGALAGALYLASKKNVLGLGRLIAYSAALFGTSLLIFSFSRIFELSLLLMAF
                     IGLGMMMQMASSNTLLQTLSDDDKRGRVMSFYTMAYMGTAPFGSLLAGGLADLIGVTF
                     TIAVGGICCMIGAALFWQKLPQLRQILHPIYVDMGIIPEVSSGLQTASDPHLPPEE"
     gene            complement(12156..12851)
                     /locus_tag="PLF20_03080"
     CDS             complement(12156..12851)
                     /locus_tag="PLF20_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOX80025.1"
                     /translation="MKFIIIGLGNFGTSLAIKLTDMGHEVIGVDKQMEKVDEIKDKIT
                     MAFCLDCKYQEAIASLPLKNTDVAIVGIGEDEGADLLTTALLKKAGVARLISRAVSPI
                     HATILEAMEISEIVRPEEETAERWAKKLSTSGIVDSFELTDKYSIIEVKAPKSFWGKT
                     IEQIGFSKNYNVIVLTIMKNVKEKNILGIFRRVTKPQIQGIAKADSVILEGDILVLYG
                     HNDDIHNLLKISR"
     gene            complement(12865..14649)
                     /locus_tag="PLF20_03085"
     CDS             complement(12865..14649)
                     /locus_tag="PLF20_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOX80026.1"
                     /translation="MNNSFIFRKNKKFSSFEKLLRIPFWLSSISILVFIYDFGFQRQP
                     NELEYLFLVYIVTIIAGCFSLIGRYILKATRPRLKSLPFDLILLVLLLCMIIQYCGIT
                     SIELPIFLQNSFWLYFATAAVFLREFSALNLSFKRTIVNPVQLFIFSFLFIIVIGTFL
                     LMLPNATYSRISFLDALFTSTSAVCVTGLTVQDTATYFTEFGQIIIIFLIQIGGLGIM
                     TFASYFSYFFRGSSTYENQIMLHEMTDTEKMAEVFGTLKRIILITFIVEATGALIIYE
                     CVGKSLIMFQSDRIFFSIFHAISAFCNAGFSTLTNNLYDPALRYNYSMQMTIALLVIL
                     GGIGFPILFNLLRFIALKLKFWFLKIIGKKPAKTAPWIINLNTRIVLNTTLLLLLVGT
                     ILFFVLEYHNTLYEHSFGGKLAISFFSAVTPRTAGFNVVNVSALHISTLLITILLMWI
                     GASPASTGGGIKTSSIAVAWLNILSIIRGKQRVEAFKREISPVSIHRAFAVIFLSIFV
                     IFLSIFFVSIFETDKGILNIAVECISAFGTVGLSTGITLHLCGASKIVLILTMFVGRI
                     NMLTILIAFFKKSSTWVGRYPSESILIN"
     gene            complement(15005..15829)
                     /locus_tag="PLF20_03090"
     CDS             complement(15005..15829)
                     /locus_tag="PLF20_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80027.1"
                     /translation="MKSLSKLVMILLGGMTLAGCSPYRELAQQYVDQSSKRAVVYLIP
                     TGYVDKVNLKRYPTLDLSAYTQYQRDSIRFEKSIFLKNLSDSIFLTRFVNSYMEELRA
                     LGVRVCLDGPDSLTCLPAEDSWIVIMDHLELREFNIPITDEQYIYPALYQKDIDLGAV
                     SLHALFSINRLNEDKKEQQLYAENYVSDEITESGFGFEKDKDKVVHYRKVQPLTLEMI
                     YNMAEALGRKYASYTYDYLLNEYIFKSSKSVPSTYYHYNRFKNKLEEAGDDRFIAK"
     gene            complement(16507..17097)
                     /locus_tag="PLF20_03095"
     CDS             complement(16507..17097)
                     /locus_tag="PLF20_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOX80028.1"
                     /translation="MKENHLLLPSPQKAKQQYVQFTQPLPSEPFEKIKMDIKFIYIRG
                     QRKNALLLTFLDTFTRLPLAWDFQYSIRHQNVAMLFDRIIEDWLQPYRPCIDGKVRVC
                     LRSDNDSRFVAKDLQDYLKSNFVDQEFILPATPQQNAHIESFNHVVEELVCKKYEFDD
                     ISQGRQVLNRFFYTYIFAGLSVQYYTCRRQCFLMNG"
     gene            complement(17426..17686)
                     /locus_tag="PLF20_03100"
     CDS             complement(17426..17686)
                     /locus_tag="PLF20_03100"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOX80029.1"
                     /translation="MKRTWNLEEKVSILKEAETNGVVETFRKHGIYATTYYEWKRKYN
                     EGGESALLLGYAKRGRKDIKKLEKENEWLKKLLVDKELELEM"
     gene            17848..18291
                     /locus_tag="PLF20_03105"
     CDS             17848..18291
                     /locus_tag="PLF20_03105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX80030.1"
                     /translation="MQIVITGISRGLGLALTKSLLQRGHSVIGISRSGKVFNELESLS
                     LQFGNAGFIGITADITQEVEWQRIYEIIQNSIDTIHALINNAGILINKPFTQMTADDF
                     DRMFSVNVRAFFFGIRTLIPLMRSGSHILNLNPPKLGNIISKKIK"
BASE COUNT         5265 a         3674 c         3695 g         5658 t
ORIGIN      
        1 tatcccgttg ttcagacgga aatctcagga ggcagatcag ccatttcggt cattccgccg
       61 cccgaaatgg ctgatctgcc tcctgagatt tccgtctgaa caacgggata ggaataaaca
      121 tatccatcca gcacgatagc cacttgccga cctatatttt cggctgtaat tcttttccat
      181 tcagcggctc cctcgcgatt catggtcatg cttacttcca cacctccggt ggtaggattt
      241 acgtcttgtc gtgcatctat aacgacttcg cctcccagag gagcctgacc atcaggtctc
      301 gttaccttta tggctactaa atcgagtatg ccagggcgct gaggatcggg tttcaccgtc
      361 cagtgaagtc gcatgttcga aggaaaaaca tccttaactc tttttagcat agcatttacg
      421 cgtgcagtgt cttttattgc agcacttccc acgcgggcag tcttcgcagg atggaactga
      481 ttgttctcgt caggataata agaagggatt aaataggcat ataaaggatg ttctttagcg
      541 tattcttcaa aagaagtctg ttttgatttt tggagtctgg cactgtcggc tagtttggca
      601 agcaagcccg tatcggcttc cgtggtttgc agggaatcag tgattccgcc ttctttgttt
      661 ttagccgaag cgattgtatc ttccggtgta atcagacctt cttccttata aattgccatc
      721 agtcgtttat cggcttcgtc gaagaattga tagacttcat cgaaattgta ggttggccag
      781 aattcgagcc gggcacttcc ctgaagtaat tttctcacac gatcggggtc tttgatgcca
      841 ggcaattcga taaggatacg ccctgtagtt tgtaattttt ggatattggg ttgagcaacc
      901 ccaaagcggt cgatacgagt gcgaagaata ttgaaagtac ggtcgacggc agctttcgtt
      961 tcctcgcgca aaacctgaag aacttcttcg ttgctggaat tgaacttcac cttgtctttc
     1021 aactcaaccg tagcgaatat ggctgcaagg cttccattag ggtctaattc cttaaatgat
     1081 tcgtagaata agtcaacaaa atcggcattg ctgttttttt gtttttcacg agctaagcgc
     1141 aaggcttcgt taaaagtcgt atctgtgctg taattcgaca gggctttcaa gatgtcgggt
     1201 acagaaactt ccatggtaac gttcatgccg cctttgaggt cgaggccaag gttgagttcg
     1261 cgttccttta cttccttata ggtgtatttt ctaactccga tgttgtaaac tacctgatcg
     1321 gcaatagaat cgaagaaaaa tttttctctt gcctttacaa gcgagtcata tacaagaagt
     1381 tcacgaaatt tatcgccttt agctatttgt ttggcaagtg cctgggcttt tgggctggtg
     1441 gcatagctct ttcccttgga ttctatatgt ttcgaaacaa aagtaaacga aagttgaaat
     1501 aagcaaacca gcacaaaaag aattgcgaaa aacttaacga tgcctttatt ctgcattctt
     1561 tgagtagatt tatagttaat gtttttttcg aaggggcaaa attagaagat tatttcattc
     1621 ctcccaattt taaattattc aaaaagatga tgtatagagt tgaattgtgt tttatagttt
     1681 catagaaatc catttgatca gatcggccca ggttattttc tttccataga gcaatattcc
     1741 ggtatgatag attttcccgg caatccaggt ggcaatgata aaagtagcca caagtaaaag
     1801 aatagacaga atcaattgcc agactggaac tccaaatgga attctcatca tcatactaac
     1861 gggtgaggta aaagggataa tagagagcca tactgccagc tggctttgag gattattgat
     1921 gataaacccc gatagaataa tggtaattaa caacggaatg gttataggaa gcacaaactg
     1981 ctgagtatct gcttcgttat ctacagctgc tgcaatagcg gcaaacatgg ccgaataaag
     2041 aagataacct cccagaaaga atagaatgaa ggcaaatagt agcaagggaa tattaattgc
     2101 tcgaatccct tcgagtgctt gggtgataat attcgattcc agcaccatat cttcattcaa
     2161 atcaaccgat tgaccagaag acactatact tgtgctggaa aaattttcta tttgtgaaac
     2221 attttgttgg atagcaggat gagttatctg aaatccaaaa atgataaccc ccgtaaacac
     2281 tatccataga acgaattgag taatgcccac cagccctacg ccaatgatct tacccatcat
     2341 caattgaaaa ggcttaaccg aagtaacgat gacttcgaca atacgattgg ctttttcttc
     2401 gatcacacca cgcatcactt gcgaactaaa cataaaaatc aacatataga tgatgattcc
     2461 tgaaagtagt cccaacgcaa tgctgatttc ctccgacgat ttgacctctg aaccgtcttt
     2521 gccaatcttt atcgagttaa tgatgatgct cgtcttgata gaagtcaata cttcagggtc
     2581 gatatttttg gccttcagtt ttaaagcttc gacctgttta cgcagatttt cctttaccac
     2641 ggttttgatg tctataccgg tttgggaggt agaatataaa atagccgtta cgggtacatt
     2701 cagctctgta cgagggatat aaaccaatgc atcgtcgccc gaagctatca atcctttttt
     2761 agcggtttcc agatcggtga agataggaac aaatcgaaaa tgctcgtcgg attgaaattg
     2821 atcgaaaaac aatcccgttt catcaagaat ctcgacggtt tttttgccct ggctcaaagt
     2881 ggttacataa ataggaacta tccagagtgc agctattaaa ataggtccca gaatcgtcat
     2941 gatgataaac gtcttttttt tgactctggt aatgtattct cgttcgatga tcagggatat
     3001 tttactcata ctcgaaaatt aggatgggtt tgccggtaaa gagccatttt gaatggtaga
     3061 aacaaagata tcgtgcatag aaggaaggag ttcgcggaat ccggtaatct gcatataagg
     3121 caaaagagaa gccaaaagat catttggagc taaagaagca tccaatttga aataatgctt
     3181 gtataagctg gaattgggtt cggttttttg ataatgaatt ccttgcggca aattcggtgt
     3241 tatagtgctt aggtcagctt tgtaagattc tacttcgaac agattctggc tgaagcgact
     3301 tttaatttcg tttactgcac cgctaagcac cactttcgac tgatgaatca atgcaatatt
     3361 gtcgcatagt tgttcgaccg attccatact gtgagtcgaa aaaatgatac ttgctccttc
     3421 atctcttaat ttcaaaattt cgttctttat caaatccaca ttgataggat cgaatccgct
     3481 aaagggctca tcgaagatca ataattcagg ttgatgaagt atagttacga tgaattggat
     3541 tttttgctgc atacccttgg aaagttcttc tacttttcta tcccaccagc tcaggatttc
     3601 gaatttcctg aaccagtatt ttagacggga tacggcttca tgttgtgaaa ctcccttgag
     3661 gcgagccaga taaatagctt gttcgctagc tttcatcttc ttatacaatc ctctttcttc
     3721 ggggagataa ccgattttat cggtatgatt aggttgcagt acctggccat tgaaaaacac
     3781 tttcccctta tcgggggcaa taatctgatt gataatccgg attagagtgg ttttaccggc
     3841 tccattgggc cccaacaagc cgaaaatgct acctttgggg actgaaatat tgatattatc
     3901 gagtgccaca tagttgccat atcgtttagt aacttcctcc gtacgtatta aatccatttc
     3961 gaggagatga cttaagagtt gaaaaattcg cgcatacgag caaagaaggt tttttccttt
     4021 ttatcgggat gaggtttgaa gttttcagaa agccgaagtt tttcgatcat ttcgcgttcg
     4081 gatgaagtaa gattctttgg tatccagatg tttagagtta ccagcaaatc gcctttttta
     4141 taggagttta cttcaggcaa tccttttcct ttgagccgga ggatttttcc tgcttgggtt
     4201 cctggttcga tctttatacg ggctttccct tcgagagtag gcacttcgac ggttgtaccg
     4261 agaattgcat cgggtatgct gatatataat tcgtaaagca ggtgattccc ttggcgttga
     4321 aaatcggggt gagggagttc ttctatcacg atcagcaggt cgcctgacag gcctccttta
     4381 ggacctgcat tgcctttgcc gctgaggctc aaagtcattc catcggttac gccagctgga
     4441 atatccactt ctacaatttc ttctcctttt acgatgccat cgccatagca ttcggggcat
     4501 ttgtcggtaa taattcggcc ttctccgcca cactggggac atgtagaagt ggtttgcata
     4561 cggccaagaa aagtattggt agtttgaatg atttgccctg tacctcggca ggtaggacaa
     4621 acatggggcg agcttccggc tctcgctcct gtgccgttgc aagccttaca tttaatgtac
     4681 ttattgactt tcagtttctt atgagcccct ttggcgattt cttccaaagt aagagaaacc
     4741 tttacccgca aattggttcc atgactggca gttttacggc tacgtgtacg cgagcttcct
     4801 ccgaatcccc caaaaccgcc aaaaccaccg aacgattcaa aaatatcacc aaagtttgaa
     4861 aaaatatcct ccatcgtcat accatggccg ctgaaacctc cgccagcagc tccgccaacc
     4921 ccttcatgcc caaactgatc gtatctactt cttttttcgg gatcgcttaa aacctcataa
     4981 gcttctgctg cttccttgaa tttctcttcc gcatccttat cacctgggtt gcgatcagga
     5041 tgaaacttca tggctttctg cctgtaggct ttcttgattt cctcccccgt agcattgcgc
     5101 gaaacttcta aaacttcata ataatctctt ttggccatat ataatctacc ttcctttcct
     5161 tccattcttt aactgccaac tatcaccttg gcaaagcgta ttactttgtc atgaagcata
     5221 tagccttttt cgattacttc gaccaccttt cccttcattt cttcattggg cgcaggtatc
     5281 atcgtcaggg cttcatgata atctgtatcg aaggtttcac ccacgttgcc gataggtttc
     5341 aatccttcct tctccaatat ttgatagaat ttattataga ttaattctat accatcgtga
     5401 tagggcttca aaacctcatc atcgttttga ttcaaagaat cgagagcacg ttcgaaatca
     5461 tccagcaccg gcaataattt gactataagg ctgctattag cgcttttaat gagttccaat
     5521 cgctcacgtg cattacgctt cttgaaatta tcaaactcgg aaaataatct taagaactta
     5581 tctttccatt cggcaatctc ttcttccgat ttttcatttg cttgttccaa ttctttcatt
     5641 ttagcctcca attctattac ttcggattca ttggttgaag tcttgccatg tttattcctc
     5701 cgtttacccc gtgttacttt atcatttaat tcctgcatcg accgatcctc atggaggtca
     5761 ttgttcgttt gctcctcatt ttcgtcgaga tggtcattat tcatagaatt tttttcttcg
     5821 ggtttcttta gatcttgtgg ttgcatatta tctggattga tcatacacat tatctgcttt
     5881 tcaacattaa tttgactctt tattcaaagt aatttcactg gtttatcttt gataaccaca
     5941 ggttgtttgc aaaaataatg cctttggaga gtaaaggtca aaatggcaga acgaagcgga
     6001 tctaaatgcg ttcaatgtca gctccgagga cttgcaggcg ggcatccaaa ttttcgtaac
     6061 ctctgtcaat ttgctctata ttttcgataa tgctttcacc tttggcagaa agggcagcta
     6121 taaggagggc gactcctgcg cgaatgtcgg gtgagctcat gcagattccg cggaggcggt
     6181 atttccagtc gagtcctatc accgtagctc ggtgggggtc gcaaagaata atgcgtgcac
     6241 ccatttcgat aagactatcc acgaagaata aacgactctc gaacattttc tgatgaatca
     6301 acacacttcc tttggcttga gtggaaacga ccagagcaat gctgatcaaa tcgggtgtaa
     6361 atccaggcca gggtgcatcc gaaatactca tgatggatcc atcacagaaa gattcgattt
     6421 cgtaatgttc cttagtaggt acgatgatgt cgttattatc ggggaaggtt tcgactccca
     6481 aacgtcgaaa aatctcggga atgattccta atgatcgata attggtattt ttaatgatca
     6541 attgggattg ggtcatggca gcaaggccaa taaagcttcc cacttcgatc atgtcgggca
     6601 tcagggtatg ggtggtactg ccaagatgtt caactccctc gatagtcagt aagttggatc
     6661 ctatgccact gatgcgtgca cccatgcgat tgagcatttt gcaaagctga actacgtaag
     6721 gttcgcaagc tgcattgaaa agtgtggtag taccctcagc cagtgtagct gccatgacga
     6781 tattggcggt tccggttacc gaggcctcgt cgagcagaat ataagttccc tttagtttac
     6841 tggcttcgag gaaagggata tgagtggcgg cattttctcc aatctttgct ccgagttttt
     6901 gaagtccatt aaaatgtgta tccactctac gacgtcctat cttatcgcca ccaggcctgg
     6961 gagaataagc cttgccaaat cgtgcgagta caggcccaac caccatcatc gaacctctca
     7021 aggccctggc tttttgatga tactctcggc tttgcatgta ctcaggatgt atatcgtcgg
     7081 cttgaaaact ccatgaatgt tctccaattc ttttcgcttt catcccgagt ccttcgagca
     7141 gttcgataag cctcagcaca tcgagaatat gcgggacatt atggatgatc acttcttcgg
     7201 gagtaagcag ggtagcacag atcacctgaa gagcctcatt cttggctcct tggggtgtta
     7261 tttctcctga gagtgtttta ttgcctttta ttctataaac ttgtcggttt ttattattat
     7321 agtccattgt tctatttgtg ttttctcttt ttcttatttc ctggtttggc aacaggttgt
     7381 aatttttgct gctgcctgat tatttgtagt aattcattgg taggtgccaa tttaatgcct
     7441 tcggtgtcaa tttttccatc cgatagttct ttcatttgtt caagaatcac atcatcggcc
     7501 acattgtccc tattccatgt taagtaagcc attttcatac tgtttccaat cacacgtatc
     7561 atcgctttac ggtcgggatg gtcggtatca agggtgctca catagtcgat cattttctgc
     7621 acattacgac cataatgcct gtatttgatc ttgttatctg aatagggaat aggttgtggt
     7681 tttagctcaa cttgttcttt cgaaggcaaa ggataaggag catccacatc gaggcggaaa
     7741 tcggaaatga tatgcaaatg atcccagagt ttccgcttgt agtcggtata atctttttgt
     7801 tcaggagtaa tttgttccat gcattttacc agagcctggg ctgcccgcgt gcgttcttcc
     7861 ctgtcgggaa tctgtatgat atattcgacc atcttctgga tgttgcggcc atattcggcc
     7921 attttgagcg gacctcgagt agtattgtat tccatgttgt tttattcagt gaaacgaaaa
     7981 aaattgattc aaatgaggct aatagatgta aaaaatttga gttgttctta ataaagggaa
     8041 cgaataatgt ctttcttatt gttcggaaga tagtgagaga aatataatat aatcagatga
     8101 ttttcagttt tgcaaattta agtaaaagtt gtttttgacc aattccgggg aagaagactg
     8161 ttgccttttt attggcacct tctccttcga ggctaattac ctttccttgt ccaaagcgtt
     8221 cgtgtcgaac ctgcatgccg gtttggaggc aacggatcaa ttcttcagta tcggggttca
     8281 agggtgagtt cacatcctca tgagaaattt ttttaagctt cgaaaaatcg ggttgtttag
     8341 tagggatggt ttttggtgat tttacggatg caaaaggtgg attccatgaa actttttttg
     8401 cacccgattc gatgaattct tcctcgattt catcgagaaa gcggcttggt tcgcaataat
     8461 tgaggctgcc atgatgatag cgtgtttgag catggctgag gaagagtttt ttttgtgccc
     8521 gtgtaatggc tacgtaaaat aagcggcgtt cctcttccag ttcgatgcga gtacccaagg
     8581 cttggatgtt agggaagagg ttttcttcaa ggccagccac aaacacataa ggaaattcca
     8641 ggccttttgc tgaatggata gtcatgaatg aaaccttggg aacgccatcg tctttttcgt
     8701 cggcatccgt cagaagggca atctgttgca aaaacaggtc gagtgagcgg ataccggttt
     8761 gatcctcctg atcgatattc gggataacct cgtcgttttg agaaaaatcg ttgattgcgc
     8821 tgagcagttc ttcgatatta tcgagccggg caactccttc gggggatttg tcgtcatcga
     8881 gttcctttaa aattcctgaa gcatgcgcaa tttgacgggc cagttcataa gcattgtggg
     8941 tttttatctg ataatgaaat ttttttatca tagccacgaa gttgctcatt ttctctacta
     9001 tattgctcct caattgcaag ccatactcga gtggattgga aattacttcc caaagcggga
     9061 catttctttc gatactgcaa accatcgcct tatccatagt gccttcgcca attcctcgag
     9121 ccggataatt gatcactctg cgaagggcat cttcgtcatt cggattgacc accaaacgga
     9181 aataagccaa aaggtctttg atttcctttc ttttataaaa tgatagtcca ccatagattc
     9241 tgtatgggat attcaagcgc cgcaaggctt cttcgatggc gcgcgactgt gcattagttc
     9301 gatacaacac ggcaaaggca tcactttcga gctggcggtt cattttcaat tcaaaaatag
     9361 tgtcggctat aagctggcct tcttcattat cactacttgc cctgattatc tttatctttt
     9421 cgcctgcttc attatctgtc caaacttttt tatatatctg ctctctgttg ttggcaatca
     9481 ctttatttgc ggcttctaca ataatcttcg tggaacgata attttgttcg agtttgaaga
     9541 ccttgtaatc aggataatcg ttcttgaaat tgaatatatt ctggatattg gctcctctga
     9601 atgaataaat gctttgggca tcatctccca caacacaaat attctcgaag cgagctgcca
     9661 gttgtttgat gatgatatat tgcgagaaat tggtatcctg atattcatct acaagaatat
     9721 tcctgaagcg gttctggtat ttgaatagaa tttctggaaa agtttgtagg atacggttca
     9781 tattgaacag caggtcatca aaatccatgg cttgagaacg gaaaagcctg tcctgatata
     9841 ttttatagag tattccggtt tcagggcgat tagcttcacg gtcggcggtt tgaatctcgg
     9901 tagaatcgag atattcctgc acagaaatca atctggattt agctgcagag attcgtgtga
     9961 gaacccatga aggggaatag atcttgaggt cgaggtgcat ctcgtcgata atggatttca
    10021 ttaggcgttt cgaatcttcg gcatcataaa tagcaaaagc cgccggatat ccgatgagat
    10081 ggccttctac tcgaagaata cgggcaaata tggaatgaaa tgtgcccatc catacattca
    10141 cagcttcgct cttacccacc aactgcagca tacgttcctt catttcacgg gctgccttat
    10201 tggtgaaagt aagtgccaag atatgaaagg ggtcgatgcc ctgttgaatc atatacgcta
    10261 tacgataagt aagtacacgg gttttgcctg aacctgcccc tgcaattacc atcacaggcc
    10321 cttcgatgca tcgaaccgct tctcgctggg cttcattgag atgtgataaa atatccttct
    10381 ccacctaaat attatttcgt aaatgcaaaa ttagatgttt taagtctttt acaattaaga
    10441 attccttaat aatttacaaa attcaatttt ataaaaaata ttcgtaaaat agtctttcct
    10501 gagaatattt aaatttctca aataaaaaat aatgtggatt gaaatatttg tggtcattta
    10561 atataaaaca ttgaaataca ataaataata attcctctaa atatgccatt tagattttgg
    10621 aaagataaaa aaattatttt ttaaatacaa taaaattagg atggatttca agaaaatttt
    10681 tccaaggcaa atggaatcat tcttcagggg gcaggtgggg atcggaagcc gtttgaagcc
    10741 cggaggatac ttccggaatg atgcccatat cgacataaat aggatggagt atttggcgaa
    10801 gttgaggcaa tttttgccaa aaaagtgccg cacctatcat gcagcatatt ccgcccactg
    10861 ctatggtgaa ggtaactcca attaaatcgg ccaggccgcc ggccagcaag cttccaaatg
    10921 gggcggttcc catataggcc atagtataaa agctcattac cctccctcgt ttgtcatcat
    10981 cagataatgt ttgtaacaat gtattgctcg aggccatttg catcatcatt cccagtccaa
    11041 taaaggccat cagtaaaagc gaaagttcga atatacgcga aaacgagaat atgagaagac
    11101 ttgtcccaaa taatgcagct gaataggcta tcaaacggcc taatcccaac acgttcttct
    11161 ttgaagccag gtaaagtgca ccggctaatg cccccaaacc cgaagccccc atcaagaatc
    11221 caaaggtata agatcctcca tgaagaattt cttttgcaaa tactggcatc aatactgtgt
    11281 aaggcattcc cattaggctt accagagcta ataataaaat aatatgtttc aatactttat
    11341 gtccaaaagt atagaagaat ccttccttta actctattag aacatgagat gaattctttt
    11401 catgcgtttt tagaggaatt cgcatcatta gtaaggaagt aactacagga atgtaactta
    11461 gtgcattgat gaggaaacac atcccctcgc ctgtaaacgc aattaacacg ccagcaatcg
    11521 aaggtcccaa taatcgagcc gcattcacca tagaagaatt caaagcgata gcattgggca
    11581 agtcgtcttt attgtcgata atctcgacga aaaacgattg acggacagga gtgtcgaatg
    11641 cgttgataca tcccagcaag atgttcaaaa caaggatgag ccatatattg atcactccga
    11701 ataaaatgag aaaggctaaa ataaaggctt gaagcattgc gaagacctga gtagcaacca
    11761 atagatggta acggttcaaa cgatcggtga ttacgccagc aaacggagcg ataaaaaaca
    11821 taggaatctg tccggcaaaa ccaactaatc ccaataggaa agccgatttg gtcagatcat
    11881 aaaccagcca gggcagggca attcgctgaa tccatgttcc aatcaaggag atgctctgtc
    11941 ctatgaaaaa caaacgaaaa tttctatagt ggagcgatcg gaaaatattc ttcaaaactc
    12001 ccgtgttaat atttgactcc atcgaaaact tcgaatgctt catcgtcatt tttttgttcg
    12061 attttttcct cttacaaaac ttctgggaag tttgacatcg gaaataaaga aaggattaaa
    12121 gggcgatgca cggaggggat cgccttttga tttgtttatc gactgatttt tagcaggttg
    12181 tgaatatcat cattatgtcc ataaagaaca agaatatcgc cttccaaaat taccgaatcg
    12241 gctttagcaa tcccttgaat ctgaggcttg gtgacacgtc tgaaaatacc cagaatgttt
    12301 ttttctttca catttttcat aatggtaagg acaataacgt tataattctt gctaaaacca
    12361 atttgttcta tcgtttttcc ccagaagctt ttaggagctt ttacttcgat gatactgtac
    12421 ttatctgtta attcgaatga gtcgactata ccgctggtgg aaagtttttt agcccagcgt
    12481 tcggcagttt cttcttctgg tctgacaatt tctgatattt ccattgcctc cagaatagtt
    12541 gcatgtatag gagatacagc ccggctgatc aaacgtgcaa ctcctgcttt ttttaataga
    12601 gcggttgtaa gcaaatcggc cccttcatct tctccaatgc caacaatcgc cacatctgta
    12661 tttttaagag gtaggctggc tatggcttcc tggtatttac aatccaaaca aaaagccatg
    12721 gtaattttat ccttgatttc gtctactttt tccatttgct tatccacacc aattacttca
    12781 tgacccatat cagtaagttt tatagccaga gaagttccaa aatttcccaa tccaatgatg
    12841 ataaatttca taatttaaac gcatttagtt gattaagata ctttcagaag ggtaacgtcc
    12901 aacccatgta gatgattttt tgaagaatgc gatcaaaata gtaagcatat ttattctacc
    12961 aacaaacatg gttaaaatta atacaatttt gctagctccg cacaaatgaa gcgtaattcc
    13021 ggtactgagg ccaacagtgc caaaggccga tatgcattcg acggcaatat tcaggatgcc
    13081 tttatcggtc tcgaatattg atacaaagaa tatggatagg aaaataacaa aaatggataa
    13141 gaaaatgacg gcgaaggctc gatggatcga aacaggagaa atttcacgtt taaaagcctc
    13201 cactcgttgt ttccctcgaa taatacttaa aatattcaac catgccacgg caatagaact
    13261 cgttttaatt ccaccgcctg tcgaagctgg cgaagctcct atccacatca acaaaatggt
    13321 aatgaggaga gtagaaatat gtaaagctga tacattcact acattgaatc cagcagttct
    13381 gggagttaca gcgctaaaga atgagattgc caattttcct ccaaaactgt gttcatacaa
    13441 ggtattgtga tattctaaaa caaaaaataa aatagtgcct actaatagca acaacaaggt
    13501 agtattcaat acaattctgg tgtttaagtt aatgatccag ggagcggttt tggctggctt
    13561 tttgccgatg atttttagaa accaaaattt caatttcaat gcaataaatc tcaataaatt
    13621 gaaaagaata gggaacccaa ttccacctaa aataaccaat aaagcaatgg tcatctgcat
    13681 ggaataatta tatcgcagcg ccggatcgta aagattgttg gtcaatgtag aaaaccctgc
    13741 attgcaaaac gcagaaattg catgaaatat ggaaaagaat atacggtcgg attggaacat
    13801 aataagagat ttaccgacgc actcataaat aattaaagcc cctgtggctt caacaataaa
    13861 cgtaattaaa ataattcttt ttaaagtgcc gaaaacttcg gccatttttt cagtatcggt
    13921 catttcatgc agcatgatct gattctcgta tgtggaagat cctctgaaaa aataactaaa
    13981 ataactggca aatgtcataa tccctaatcc accaatctga atcaagaaaa tgatgatgat
    14041 ttgtccgaat tcggtaaaat aagtagccgt atcctgaaca gtaagtcctg ttacacaaac
    14101 tgcactggtt gacgtaaaca gggcatctaa gaaacttatt cgtgagtaag tggcattggg
    14161 caacattaat aaaaatgtcc ctataacgat aatgaaaaga aaactaaaaa tgaataattg
    14221 tacgggattt acgattgttc gtttaaaact gagattcaat gccgaaaatt ccctcaggaa
    14281 taccgctgcg gtggcaaaat ataaccaaaa tgaattttgt aaaaatatgg gcaactctat
    14341 ggatgtaatt ccacaatact gtataatcat gcataaaagc agaaccagta aaattaggtc
    14401 gaaaggcagg gattttaacc ttggacgagt tgcctttaga atatatcttc caatcaaaga
    14461 aaagcatcct gctatgatag taacaatata aaccaggaat aaatattcga gttcattggg
    14521 ttgtcgctga aatccgaaat cataaataaa tacaagaatt gatatcgaac tcagccaaaa
    14581 tggtatgcgc aggagtttct cgaaacttga aaattttttg ttttttcgaa aaatgaaaga
    14641 attattcact gaaacaaaat cttttcatga tcacatcagt cgtgataata ttgcataata
    14701 ttcttcttgc cgaaaaaaga atgtaaacca ttgcaatatt acgaaattta tccttcgatt
    14761 ttatgatggt tcatatttac tctctgtttt catcgatatc ggagtaattg cccttctgtc
    14821 ccttgttact cgagaatatg gcaaatttgc agtgtcatgt tataagtgta ttcttagtct
    14881 gcgtaaaagt gcacatccat acttcgggga aaaatcaacc ttcttttaaa cgagaaacag
    14941 cagtaaaatg ggcgaatagc gctctttctt cttcttcaat gttcaggtgt cgagaggcag
    15001 aggtttattt tgcaatgaag cgatcatcgc ccgcttcttc gagtttattc ttgaacctgt
    15061 tataatgata ataagtggaa ggcactgatt tgctgctttt gaagatatat tcattcaaca
    15121 ggtaatcata ggtataagaa gcgtatttac gacccagagc ctcggccatg ttgtagatca
    15181 tttcgagagt caagggttgt actttgcgat aatgaactac tttatcctta tctttttcga
    15241 acccaaagcc actctcggtg atctcatcgc ttacgtaatt ctcggcatat aactgttgct
    15301 cctttttgtc ttcattcaaa cggttaatcg aaaacaaggc atgcaaggag accgccccca
    15361 gatcgatatc tttctgatat aaagcaggat aaatgtattg ttcgtccgtt atggggatat
    15421 taaactctcg taactcgaga tgatccataa ttactatcca tgaatcttct gctggaaggc
    15481 aagtgagtga atcgggacca tcgaggcaga ctcgtacgcc taaagctcta agctcttcca
    15541 tgtaagaatt tacaaatcga gtcagaaaaa tcgaatcgga taaattcttc aagaaaatac
    15601 tcttttcgaa tctaatgcta tcacgctggt actgtgtata tgccgataaa tcgagagtcg
    15661 gatatcgttt cagatttact ttatcgacgt atcctgtagg gattaaataa acaacagcgc
    15721 gtttcgacga ttgatccacg tattgttgag ccaactcccg gtagggactg caccctgcaa
    15781 gtgtcatccc tccaagcaat atcataacca atttcgatag acttttcata gcactatcct
    15841 tttgttttat ggatttgaaa taatgcactt caagtaataa aaagaacctt ctaatttcgt
    15901 gtcaaatatg caaaaaattt gccagagatg tcagaatggg gacagtggga ctgaaaatag
    15961 ggaatgaagg aatggaggga tggagacgag gggatgagag gatggtgaat ggtgaatggc
    16021 gattagtgag ttaaaagttg aaagtttata aaggtaaaaa cgaaagaagg gagaaaggga
    16081 ggaagggagg aagggaggaa gggaggaagg gaggaaggga ggaagggaga cgtggggact
    16141 aggagactga gagacgaggt ggattggtga tggtgattgg taagttaaaa tttataaagt
    16201 taaaagtgga gaaaggggga cgaggagaca gagagactgg aatttgggat ttcgaatttg
    16261 taatttatag tcgtaagttg tatgttgtta attgtaagtt gttggttgtg gcttaacccc
    16321 ctattgttag aactaaatta gtgatgtttt aacaaattta tcatcttttt tagtaatttt
    16381 gttattaagc tcttcataaa attcttcaga cagggcagag agccattttg gcctctgtcc
    16441 tctgaagaaa aatgtttgtt ttgttctgtt ccctttcttt ttgtttatta ccccgatatt
    16501 tccttttcag ccattcatta aaaaacactg ccggcggcaa gtataatatt gaactgatag
    16561 tcctgcgaaa atataggtgt aaaagaatct attcagtacc tgccttcctt gtgagatgtc
    16621 atcaaattca tattttttgc ataccagttc ttctactaca tggttaaaac tttcgatatg
    16681 ggcattctgt tgaggggttg caggcaaaat gaattcctga tccacgaagt tactctttag
    16741 ataatcttga agatcctttg caacaaatct tgaatcattg tcgctacgca ggcatactct
    16801 tacctttcca tcaatacaag gtcgataagg ttgaagccaa tcttcaatta tcctgtcaaa
    16861 aagcatagcc acattttgat gccgaatgga atattgaaaa tcccatgcaa gaggtaaacg
    16921 ggtaaaagta tccaagaagg taagtagaag agcattttta cgttgtccac gtatgtaaat
    16981 aaatttgatg tccattttaa ttttttcaaa tggttctgaa ggcaagggct gagtaaattg
    17041 tacatattgc tgtttagcct tttgaggtga aggaagcaat agatgatttt ctttcatcaa
    17101 acggtatgtt ttgtttttac caatggcata gcccctatct ttaagtagga aaccttaaaa
    17161 tagccatttt ccataaattt tccactcagt gtagtgcgaa tatcttctat tacttcttca
    17221 tttgaaatca accacatacc atcttttaaa caatgtgtag aagctgtcct accccccttt
    17281 acccccacgg ggtcgctagt aataggtgct tttactcata cctgctattg ctgatgcttt
    17341 atctgctttc atacctcttg ctataaattt cctgattatc gcttcacgtt cttccattgt
    17401 tccatctttt ttttaaaatt tccgtctaca tttctaattc aagttccttg tctacaagta
    17461 gcttttttaa ccactcgttc tccttttcaa gttttttgat gtcttttctt ccccgcttag
    17521 cgtaacccag caaaagtgca gactctccgc cttcgttgta tttccttttc cattcataat
    17581 aggttgtggc atatatgccg tgctttcgaa aagtttccac aacaccatta gtttcagctt
    17641 ccttaagaat cgaaactttt tcttctaaat tccatgttct tttcatattc taaaattagt
    17701 gtttttaatc actaatcgag tccaaagatt taaagggcta agacaagaca acatacaaat
    17761 caactaacat tctttcagag attcaccaaa taaccatatc acagtaactt tggtctaact
    17821 ttattcattc attaaatttt tcaaaacatg cagattgtta tcacgggaat aagcagggga
    17881 ttgggactgg ctttgactaa atctttgctg caaagaggtc atagcgtgat tggaatttct
    17941 agatcgggaa aagtttttaa tgaactcgaa agtctttctc tccaattcgg caatgccggt
    18001 tttattggaa ttacggccga tatcactcag gaagttgaat ggcagcggat ttatgaaatc
    18061 attcagaata gcattgatac tatccatgcg ctaatcaata acgctggaat attgattaat
    18121 aaacctttca ctcaaatgac ggcggatgat tttgatcgta tgttttcggt caatgtaagg
    18181 gcttttttct ttggcattcg aaccttaata cccctgatgc ggtcgggctc gcacatatta
    18241 aacctaaacc ctccaaaatt aggtaatata atttcaaaaa agataaaata at
//
LOCUS       DAOORU010000026        18070 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_3783_length_18070_cov_6.496420, whole genome shotgun sequence.
ACCESSION   DAOORU010000026 DAOORU010000000
VERSION     DAOORU010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18070
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3783_length_18070_cov_6.496420"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..626)
                     /locus_tag="PLF20_03110"
     CDS             complement(153..626)
                     /locus_tag="PLF20_03110"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HOX80031.1"
                     /translation="METLQKPKGSPSYNIQDELNKFNAWLLYRRYSHNSIKTYIDALK
                     NFLLFLQPKTEADATNDDMVRFVNDYILKNGYSLSFQNQVVNACKLYFKEVAHSELDV
                     NNLKRPRREHKLPNVLSKEEVKVILDAPTNLKHRAMLSIIYACRLRRSELLNLKL"
     gene            complement(639..872)
                     /locus_tag="PLF20_03115"
     CDS             complement(639..872)
                     /locus_tag="PLF20_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80032.1"
                     /translation="MKVLGQKIIILYELSTHRNNDVVFLNFEFDKTLNEIVRRLGCAR
                     WSQTNLKCYLPCQEFNLARLRKAMDGVADIDVS"
     gene            complement(887..1231)
                     /locus_tag="PLF20_03120"
     CDS             complement(887..1231)
                     /locus_tag="PLF20_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80033.1"
                     /translation="MVLIPGYVFMRTNEVAICSDEFHLPGSRGLLIQDEKPALLSHDN
                     AMNLNRLCNLDLAPEITTIFGCEQQITIDSGPLKGISGKVIRTDGKRYLIVESGIKGI
                     LLKVNLEQNAMF"
     gene            complement(1603..2964)
                     /gene="tilS"
                     /locus_tag="PLF20_03125"
     CDS             complement(1603..2964)
                     /gene="tilS"
                     /locus_tag="PLF20_03125"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOX80034.1"
                     /translation="MNSLVEKTKKFLKDIVEHPENYRYLLGVSGGPDSMAMAKIFQEL
                     GYQMGIAHCNFLLRKAEAQEETEFVRSWANQNNFPLFINYFETAQYAEQQKISIQMAA
                     RALRYQWFEKIRHEQGYDFICTAHQLDDQKETFFINLLRGTGLNGLRGLEPLSGKILH
                     PLLFASRNEIMEFIAQEQLPYRIDSSNLHHDYLRNKIRLKVFPLLDKTYPGWSTHFEY
                     TLQRLAFAADVFEDYLRTNLKDRIIEHDDEVRVDFSGFENSKHAVAFLYEIIKGFNFN
                     YPQAIHLAEALKNSPGKKFLSPTHCIYTKGSTFCIQQLREEQRTTFSISSLEDITDYP
                     VPFKFRLYNKDNFPGFQKNPRFACFDYDRLNFPLQLRPWRQGDYFQPLGMKGRKKVSD
                     LLIHQKIPLYRKKDVWLLCDKDDEVIWVVGLRTSEHAKITDDTQNILQIEIPQEHVDV
                     LHE"
     gene            complement(2961..3548)
                     /locus_tag="PLF20_03130"
     CDS             complement(2961..3548)
                     /locus_tag="PLF20_03130"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOX80035.1"
                     /translation="MFLSLQNIDIDLLFLINGLHAPWLDNLMWQISHTLIWLPLYAIF
                     ILLVIKLFKKDTWKILIMAALLITVTDQTTNLLKNTVKRPRPTHQPGIMENLHVVNDY
                     HGGNYGFVSGHAANTMSIAVFLTLLLKKNYRFMKWLPLSWSFIVGFSRIYLGVHYPSD
                     VLAGFALGAILGYLFFRLAFRIPHFISRPQLSETP"
     gene            complement(3570..4553)
                     /gene="obgE"
                     /locus_tag="PLF20_03135"
                     /pseudo
     CDS             complement(3570..4553)
                     /gene="obgE"
                     /locus_tag="PLF20_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109231.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
     gene            complement(4632..5801)
                     /locus_tag="PLF20_03140"
     CDS             complement(4632..5801)
                     /locus_tag="PLF20_03140"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF001381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOX80036.1"
                     /translation="MLNIVLFGPPGAGKGTQSKKLIEKYNLTYVATGDLLRQEITEGT
                     ALGMEARNLIEKGRLVPDDMILQIIEKHINANADTNGILFDGFPRTYVQAYILEGLLM
                     RMHTSLTCLLSLEVPREELIARMLKRAKMENRNDDTLEVIEYRLLEYETKTIPVIEFY
                     KERNIYFPIDGVGTVDEIFERLCQVIDNSLTKQWMNVVLLGPPGAGKGTQGEIIAKRH
                     ALGYISTGTLLRKEIKEGTEIGQRAKPYMEKGEIVPDEIAVKLIERELHLESKVRGFI
                     FKGFPRTIVQTYILDGILKKINSSVTVAIELKVPTLECFKRLTMRAKTDKACSYDKDP
                     ELIIHHLEEYKQKSILVKKYYKNQNKLVKVDGTGDVAEVSDRISEVIDKVARMSR"
     gene            complement(5816..6364)
                     /gene="hpt"
                     /locus_tag="PLF20_03145"
     CDS             complement(5816..6364)
                     /gene="hpt"
                     /locus_tag="PLF20_03145"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOX80037.1"
                     /translation="MEKTIQLHDKTFTIYIKASQIEEAIEKIAQQINEDIGHKKPIFL
                     VILNGAFLFAADLIRKYSGECEVSFVKLSSYYGTLTTTRVREIIGLENDVKGRTVVIV
                     EDIVDTGITMDNIVKKLIGLGAGEVKITALLFKPDAFRKNFKIDYLGFEIPNDFIVGY
                     GLDYNGLGRNLPDIYQIVKTNS"
     gene            6478..6984
                     /gene="purE"
                     /locus_tag="PLF20_03150"
     CDS             6478..6984
                     /gene="purE"
                     /locus_tag="PLF20_03150"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOX80038.1"
                     /translation="MKPLVSIIMGSTSDWMVMEGAANFLNEIQIPFEIEALSAHRTPE
                     KVNEFSRTAQARGIKVIIAGAGMAAHLPGVIASCTALPVIGVPVKASLEGLDALLSMI
                     QMPPGIPVATVGIDASRNAAILAAQIIALSDPEVAHRLAAFKEGLKEKVIKADDELKK
                     IKLPYKVD"
     gene            complement(6990..8168)
                     /locus_tag="PLF20_03155"
     CDS             complement(6990..8168)
                     /locus_tag="PLF20_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HOX80039.1"
                     /translation="MELDAYFNPLDKTEFESIKRGRPHFGNLLRLHTPEKGISDLSDI
                     HIALIGIPESRNAPHNAGCELGPDIIRNYLYRLFPPKSPLPMADLGNLKQGNTPEDTY
                     AGLAEVYAYLRNQDIVPIVLGGSHDMTYAMYLGYEKTGININMVNVDPFFDLGEAPEE
                     INHTTWLSKLFLRQPSVLFNYTHLGYQTYFVDSAAVTLMKNMLFDVYRLGQINPNIDN
                     IEPLVRNADLISIDISAVRASDAPGCAYATPNGFFGDQICQITRFAGMSDKVSSLGIF
                     EYNPKYDCNGRTAYLISQMIWYFVEGYLGRLNDFPGINPSLENHFRYFVKIENMEEEI
                     IFYKSKKSDRWWLQVPCPEYLQTKYYRHIIVPCSYQDYQSACNNELPDRYWQFYQKMM
                     "
     gene            complement(8257..10632)
                     /gene="topA"
                     /locus_tag="PLF20_03160"
     CDS             complement(8257..10632)
                     /gene="topA"
                     /locus_tag="PLF20_03160"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOX80040.1"
                     /translation="MAKNLVIVESPAKAKTIEGFLGKDFTVKSSFGHIRDLDKNKLSV
                     DIQNDFAPQYEISEEKEKIIEELSKVAQKAEIIWLANYEDREGEAIAWHLYEVLELEK
                     ERTKRIVFHEITKKAIQQAIENPRFIDFNLVDAQQARRVLDRLVGYELSPLLWKKVKP
                     ALSAGRVQSVAVRLIVEKEFEIKNFKPSTYFRVTGSFELLNKKDDKAQLLAELDHRFP
                     TKEAALEFLNHIMGSTYTIKNVEKKPGKRTPAPPFTTSTLQQEAARKLGFSVARTMMI
                     AQQLYEDGLITYMRTDSVNLSDLALSMAKDEIIKLYGKEYSKTRQYTTRTKGAQEAHE
                     AIRPTNMKLHQAGRNEAQLKLYELIWKRTMASQMSDALLEKTTIAISISKTNRYYFLS
                     KGEVVIFDGFLKIYSESADEEKEEMNAGILPSVNVGEEIKEKYISANQKFTTPPLRYT
                     EASLVKKLEELGIGRPSTYAPIISTIQKREYVEKKDHVGQEKTALILTSKNGKIKEES
                     KIEKWGFEKGKLTPTDIGILVTQFLMHNFENIMDYQFTARVEKEFDEIADGKLKWNQM
                     IQRFYWPFHETVVKTFETQEKVKGERLLGVDPASGKKVYAKIGRYGSIVQLGETKDPT
                     GQKPRFAGLKKNQSIETITLEEALSLFKLPRTVGFYMGKEIIVSTGRYGPYLLYSNTF
                     YSIPKDEDPLMINQEKAIQIIEEKNQKEAAKIIKTFPERPDVVIQNGRYGPYIKIGNE
                     NIPIPKKIDPQSLELQQCLELQKQYLESKASRAESEESPLTKKKTKPRGKK"
     gene            10782..12119
                     /gene="miaB"
                     /locus_tag="PLF20_03165"
     CDS             10782..12119
                     /gene="miaB"
                     /locus_tag="PLF20_03165"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOX80041.1"
                     /translation="MFENRLFIETYGCQMNFSDSEIVASILSSEGYSLVNDPHQASLI
                     LINTCSIREKAEERVWKRLRELKSLKKKNPELKIGIIGCMAERLYEELLFAGHGVDLV
                     AGPDAYRFLPELLSNTLQGAQAANIQLSFEETYEDIEPLRLDPSGVSAFISIMRGCEN
                     FCAYCVVPLTRGKERSRHPETILNEARNLFQRGYPEITLLGQNVNSYYFKNAHLSMNF
                     SDLLAKVAEIHPSLRVRFTTSHPKDLSDKLISTIASYPNICKSVHLAVQSGSDAILKR
                     MNRKYTRNEFLDRVRSIRKMIPESTISTDIIAGFCGETEEDHRQTLSLMEEVGFDSAF
                     MFRYSERPGTLAARKYPDDVPSEVKARRLQEIIDLQHWHSLRSNRQDIGKTFEVLVEG
                     SSKRSRAMLSGRNSQNKMVVFEPKKATRGQYVRVRITGCTQATLIGEEMEDDL"
     gene            complement(12225..13289)
                     /locus_tag="PLF20_03170"
     CDS             complement(12225..13289)
                     /locus_tag="PLF20_03170"
                     /inference="COORDINATES: protein motif:HMM:NF037527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80042.1"
                     /translation="MDTLDKIKNTHADVCVTIIMNTHQTRPAIQQDMLTLKNLTREAE
                     ERILSLCDPKTAESILHQLKNTINSIDFSRNDKSLILFVCHEFGQYLTLPVSVKNRVV
                     IDKTFATRDVYRAMHETAAYYVLALSRKKARLIKAINNQGEELKIQPFPLYNEGFNEQ
                     KAKLSPDEIQESLIEEFFNNVDKALWEVIRNSDLPVVIASEIRNYNIFLKLSRHKKKI
                     IGRVPLLHEDAKTHQIVAEAWQLVKKNLEDQTRKQLSRLEKAINNQNLLTDYTEVWNA
                     IQQGRGRTLFVKKGFHQPAVLLNGHVALVEKVMKDAPSVIEDIIDEMIEKMIEQQGEV
                     VFIDGDDLDRFNGIALIVKS"
     gene            13597..15756
                     /gene="rnr"
                     /locus_tag="PLF20_03175"
     CDS             13597..15756
                     /gene="rnr"
                     /locus_tag="PLF20_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOX80043.1"
                     /translation="MAKKGRDKRNKADSLSTHILAVFSADPFRSYNYKQVAAAMGIKD
                     KATRDLIFSTMLEMLKEGILQEVKRGKFRLDLSRFDQIVPASRYIIGTVDMKQTGKAY
                     IVPREPGEDIYISPSHTGKAFHGDTVKVFLFPRRPGRKLEGQVVEVLKRAKTHFVGKL
                     QLSDKYAFVIPDNPSMAVDFFIPREYLNKAKDGEKVVVELMEWPEYARNPFGRIIKVL
                     GKPGEHQVEMQSILVEWDFPIGFSREAEKEAKAIPKAIPPEEIEKRRDFRKVFTITID
                     PADAKDFDDALSFRQLEGGLYEVGIHIADVAHYVRPGTAIDQEAERRGTSVYLVDRVI
                     PMLPEKLSNFVCSLRPNEDKLCFSAVFVMDREAHITSQWFGKSIIRSDYRFNYDEVQQ
                     IIEGGQHAVRDEIIALYKLSAKLRAERMKKGTINFETKEVKFDLDDKGKPLGVYIKEQ
                     KEANWLIEDFMLLANRRVAEWVGHTKGKTRSKTFVYRVHDEPPAEKINQFAQFVSKLG
                     YSIKLGSRASFARSLNRLFTEVAGKGEENLIETIAVRTMAKAYYSTDNIGHYGLAFDF
                     YTHFTSPIRRYPDMMVHRLFWDYLNGAGSADKEFYEERCIHASEMERKALEAERSSVK
                     LKQAEFMKDKVGQIFDALISGVSKYGIFAEIVDNYCEGMIRLRDLTDDFYYLDEDNYQ
                     VIGQRYGETYRLGDPVKVIVKNVDIQKKQIDFKLVKS"
     gene            complement(15766..17205)
                     /locus_tag="PLF20_03180"
     CDS             complement(15766..17205)
                     /locus_tag="PLF20_03180"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOX80044.1"
                     /translation="MKNTIRFCRAFLIVMACIFSIGISFSQTEYGAFTATGSGMPVIV
                     VSDYQCLGINPANLGWNADNHKWHFGIVGLSVSGYTDAVSKSDLNLLYEDHEYTLAEK
                     QEAARRFSDSRLSFDVGMNGIGVSYQDPDIGGFAFTMRERGSFNMHLNSTAAELLWLG
                     FNSDYFDYKVIEQGLTIAGISTNPKPVAELFRGSSFSSAWLRDFILGYGRQIVEIKDW
                     SLYGGLSMKYTMGFAWVDIFVNKDKFFGYSSMSPVFKFEYPQPTPSLDTNGKLRPSGH
                     GFAFDIGLSGRYKNLWRFGLAINDIGTMTWNKNVYSASFEATIRKIETPGLGNENINE
                     VFDAIVIEDDLMQWEGLNDKKASYPSQMRLGAAYTGVPHIIIGSEVMIPLNKVPGAQE
                     RPVYSLGMQASPLNKFFISAGFQTGGNYDFHIPIGFSIKLVPGWEIGLATQDISVLFR
                     NVSPYLSLSFGFMRFSFTQYERSKEVPQE"
BASE COUNT         5042 a         3645 c         3831 g         5552 t
ORIGIN      
        1 actttttggc cttcgaaaag ccaggtaacc ggcttgtatg ccacaaagta gttgcacagc
       61 atggttatgg ttttctccga aataggtaca accctgtcct tccttccctt tgcctttcta
      121 atgagaagca ggtgccgttt cgaatcaaca tcctatagct tcagattcaa caattcactt
      181 ctacggagcc tacaggcata tataatgctc agcatggctc tatgcttcaa atttgtaggt
      241 gcatctaaaa taaccttcac ctcttctttg ctgagcacgt tgggaagttt atgctcgcgt
      301 cgtggtcgct tcaaattatt aacatccaac tcgctatgcg cgacttcttt aaagtaaagc
      361 ttgcacgcat ttacaacttg attttgaaaa gaaagactat atccgttttt taaaatgtaa
      421 tcgttaacaa agcgcaccat gtcgtcattg gttgcatctg cctctgtttt gggttggagg
      481 aatagtagaa agttttttag tgcatcaata taagttttaa tcgaattatg gctataccgc
      541 ctgtacaaaa gccatgcatt gaatttattc aactcgtcct gaatattata cgaaggggag
      601 ccttttggtt tctgaagtgt ctccatgttt tcatttaatt aggaaacatc tatatctgcc
      661 acaccgtcca tcgccttccg aagcctggca aggttaaatt cctggcaggg aagatagcat
      721 ttaaggtttg tctggctcca tctggcacag ccaagccttc gcactatttc attcagagtt
      781 ttatcaaatt cgaaattcag aaatactaca tcgttatttc tgtgggtact taattcgtaa
      841 aggattatta ttttctgccc taatactttc attgtatgct aatttcttag aacatcgcat
      901 tctgttccag atttactttc agcagtatgc ccttgatacc gctttccact atcaaatatc
      961 gcttgccatc ggtacgaatg accttgcccg aaattccctt caaaggcccg gaatcaatcg
     1021 taatctgttg ttcacaaccg aaaatagtgg ttatttcagg ggctaaatcg aggttgcata
     1081 aacgattcag attcattgca ttatcgtgag acaacagtgc aggtttctca tcctgtatca
     1141 acaggcctct gctgcccggc agatggaact cgtcggaaca aatagccacc tcattagtcc
     1201 tcataaatac atagcccgga atgagtacca taataatatt ttctttcttt cccgcacgaa
     1261 aacgccaatg tttaatttcg ggtagttagg catcgaaacc tttcgtagaa gctgtactac
     1321 caaaattctc tctttctttg ccagagaacg caggatgagc catgactttg gcacagaatc
     1381 atcggggtat gtgccgggct tataactccg ctttatgttg gaattgtaca tcccaacaag
     1441 aggatatatt tttgcctgct cacaatgtga tacatcttca acgaggaaat ataaatagaa
     1501 ttttcaaatc aaactgtgat gaaaattttt cttccatcaa tggagaaaaa gggcaaggga
     1561 taaaacatca caaaaggaag aacctcaaca ttgtatgggt gtttattcat gcaacacatc
     1621 cacatgctcc tgaggaatct ctatttgcag aatgttttgg gtatcatcgg taattttggc
     1681 atgttccgaa gttcgcaagc caactaccca aattacttca tcatctttat cacacaataa
     1741 ccaaacatct tttttacgat acaggggtat tttttgatga atgagcaggt cgcttacttt
     1801 tttgcgtccc ttcattccta ggggttgaaa ataatcgcct tgccgccatg gtctcaattg
     1861 caatggaaaa ttcaagcggt cgtaatcgaa acaggcaaaa cgggggtttt tttgaaaacc
     1921 cggaaaatta tccttgttgt atagtcgaaa tttgaatggg actgggtaat ctgtaatatc
     1981 ttccaatgaa gagatggaga aagttgttcg ttgttcttcc cttaattgct ggatgcaaaa
     2041 ggtgcttcct ttggtataga tacaatgggt tgggctcagg aattttttcc ccggactgtt
     2101 tttcaaagct tcggccagat ggatggcttg agggtagtta aaattgaagc ccttaataat
     2161 ttcatagaga aaagccacag catgtttcga attttcgaaa cccgaaaaat ctactcttac
     2221 ttcatcatca tgttctataa tcctatcttt caaattcgtg cggagataat cctcgaagac
     2281 atcggcagca aatgcaagcc gctgaagtgt atattcgaag tgggtgctcc agccgggata
     2341 tgttttatct aacaatggaa aaactttcag tctgatttta ttacgcagat aatcgtggtg
     2401 gaggttggac gaatcgattc gatagggaag ctgttcttgc gcaataaatt ccatgatttc
     2461 gttgcgagaa gcaaaaagca agggatgaag aatttttccc gatagtggtt ccaatcctct
     2521 taaaccgttg aggccggtac ctcgtagcaa gttaataaaa aaggtttcct tttggtcgtc
     2581 gagctggtga gcagtacaaa taaaatcata gccctgttca tggcgtattt tttcaaacca
     2641 ttgataacgc aacgctctgg cggccatttg gatggaaatt ttttgctgct cagcgtattg
     2701 agctgtttca aaataattaa taaacaaggg aaaattattc tgatttgccc aagacctcac
     2761 aaattcggtt tcttcttggg cctcagcttt tctgagtaaa aaattgcagt gtgcaatacc
     2821 catttgatat cccagttcct gaaaaatttt ggccatggcc atactgtcgg ggcctccgct
     2881 gacacctagt aaatagcgat agttttcagg atgttcaact atatccttta gaaatttttt
     2941 tgttttttcg acgagtgaat tcatggggtt tcagacaatt gaggtcgaga aataaaatga
     3001 ggtatcctaa aagctagccg aaaaaacaaa taacctaaga tggctccaag ggcaaaacct
     3061 gctagaacat cgctgggata atgaacgccc agataaattc tgctaaaacc cacaataaat
     3121 gaccatgaca gaggcaacca tttcatgaag cgataatttt tttttagcag caatgttaaa
     3181 aaaactgcta tactcatggt atttgccgca tgtcccgata caaaaccata atttccacca
     3241 tgatagtcgt tcacgacatg aagattttcc attatacctg gttgatgggt cgggcgagga
     3301 cgtttcacgg tgtttttcaa taaattagtg gtttggtcgg taaccgtaat caataaggct
     3361 gccataatca agattttcca tgtatctttc ttgaataatt tgataaccaa caagataaaa
     3421 attgcataaa gcggcaacca gatgagagta tgactgattt gccacatcaa attatccagc
     3481 catggagcat gtaagccatt gataaggaaa agcaaatcaa tatctatgtt ttgaagagaa
     3541 agaaacattt aatgaatggg gtttgaggtg agttgtttcc agatgaggtc tttcagttct
     3601 tccaatcctg tttgcgctac tgatgaaatg aaaacatggg gtaagtcggc agggatattt
     3661 tttttaagat tttcgagtga atgttcatca acgaggtcgg ttttggtgat ggcgagaatt
     3721 ctgtccttgt cgagaagttc ggggttatat tgttttagct cgttgagcag gatatgatat
     3781 tcgttggaaa tatcttggct tgtaacgggg atcatgaaaa gaaggagtga attgcgttcg
     3841 atatggcgta ggaagcggag gccaaggcct tttccctgat gagctccttc aatgataccc
     3901 ggaatatcgg ccataacgaa ggaatgatgg tctctataat ttaccagtcc cagattaggt
     3961 tgcagagtag tgaaaggata atcggcgatt tcgggtttag cagccgatat gaccgaaagt
     4021 agggtagatt ttccggtatt gggaaaaccc acaaggccga catcggccag cagcttaagt
     4081 tctatgatat accaaccttc tattccggct tctccttcct gtgcaaaacg aggcgtttgc
     4141 tgtgtagccg ttttgaagaa atcatttccc ataccacccc ggcctccttc taaaagaata
     4201 acttcctgcc catcttcaac gagttcggcc agaatttcac ctgtttgagc atcttttatc
     4261 gcgcaaccta cgggaacttc gataatttta tcttcaccgt cggctcccgt acgacgattt
     4321 ccagcaccag gattgccatc tcccgcaaag acgtgtttag tatatttcaa aggcaacaaa
     4381 gtccataatt gtgcattagc gcgcaggatg atatgacccc ctcttcctcc attaccccca
     4441 tcaggcccac ctttgggaat gtatttttcg cgtcgaaaat gtacagaacc accccctccc
     4501 ttacctgagc gaagataaat ctttacataa tctacaaaat taggcgatga catgataaat
     4561 tcttaaccca atgcaaagtt aatcgaaagc tagtgaacct ctatgctatc tataaactaa
     4621 accacaaaac cttatctact cattcgggct actttatcaa tcacctctga tatgcggtcg
     4681 gacacttcgg ccacatcacc tgtgccatct acctttacca atttgttctg attcttgtag
     4741 tattttttaa ccaggatgga tttttgttta tattcttcga ggtggtggat aattagttcg
     4801 gggtctttgt cgtaggagca tgccttgtcg gtttttgccc gcatggtaag acgtttgaag
     4861 cattcgagtg tagggacctt cagctcgatg gcaacggtaa cgctcgagtt gatttttttc
     4921 aatattccgt cgagaatata ggtttgaacg atggttcgtg ggaacccttt gaaaatgaaa
     4981 cctcttacct tcgattcgag atgaagctca cgttcgatga gttttacggc aatctcatcg
     5041 ggaacgattt ctcctttttc catgtagggt tttgccctct ggccaatttc ggttccttcc
     5101 ttaatttctt ttctaagtag ggtacctgtg gagatatatc ccaaagcatg gcgtttggca
     5161 ataatctcgc cctgagtgcc tttgcctgct cccggaggac ctaacagcac tacattcatc
     5221 cattgtttgg tgagagaatt atcgatgacc tggcacaatc tctcaaaaat ttcatccaca
     5281 gtacctactc catcaatagg aaagtagata tttctttcct tatagaactc aatgacgggt
     5341 attgttttgg tttcgtactc gagcaagcga tattctatga cctcgagggt gtcatcgttg
     5401 cgattttcca tcttggctcg cttaagcatt cgggcaatta attcctcacg tggaacttcg
     5461 agacttaaaa ggcaagtcaa gctggtatgc atgcgcatga gcaagccttc gagaatataa
     5521 gcctgaacat aggttcttgg aaaaccatcg aacagtatcc catttgtatc ggcgttggcg
     5581 ttgatatgtt tttcgatgat ttgaagaatc atatcatcgg gtactaaacg acctttttcg
     5641 ataagatttc tggcttccat acctaaggca gtgccttcgg taatctcttg acggagcaag
     5701 tcaccggtgg caacataagt caaattgtat ttttcgatga gttttttcga ttgtgttcct
     5761 ttccctgcac ccggagggcc aaataataca atgttgagca tagggcttaa ttaatttacg
     5821 aattggtttt aacaatttga tatatatcgg gcagattccg tcctaatcca ttgtagtcga
     5881 ggccatagcc aacaatgaaa tcattgggaa tttcgaatcc cagataatcg attttgaagt
     5941 tttttcggaa agcatcgggt ttgaaaagca aagccgtaat tttgacttct ccagctccca
     6001 atcctatgag cttcttcact atattgtcca tggtaattcc tgtatccaca atatcttcta
     6061 ctatcacaac cgttcgtcct ttcacgtcgt tttccaggcc tatgatttcg cgcactcgag
     6121 tcgtggttag tgtaccataa tatgaagaaa gttttacaaa tgatacttca cattctcctg
     6181 aatatttcct tattaaatct gctgcaaaaa gaaaggctcc gttcaaaatg accagaaaga
     6241 tgggtttttt atgaccaatg tcttcgttga tctgctgggc aatcttttcg atagcctctt
     6301 cgatttgtga tgctttgatg tatatggtga aggttttatc gtgaagttgt atagtttttt
     6361 ccatggttgc agaatttttg tttgaaattt gttctacatt tggacattaa ggaactcctt
     6421 atgcaaaata acgataaaat tctctgattc ggttcatttt taacaaattc attcattatg
     6481 aaacctttag ttagtattat tatgggaagc acttccgact ggatggtaat ggaaggggct
     6541 gcaaattttt tgaacgagat tcagatccca ttcgaaatcg aggctttatc tgctcacaga
     6601 actcctgaga aggtgaatga gttttctcgc acagctcagg caagaggaat aaaagtgatt
     6661 attgctggag ctggcatggc ggctcatttg cctggtgtaa ttgcatcctg caccgcttta
     6721 ccggttattg gagttcctgt aaaagcttcg ctcgagggat tggatgctct gctttcgatg
     6781 attcaaatgc ctccgggaat ccctgtagct actgtaggaa tcgatgcatc ccgcaatgct
     6841 gctatactcg ccgctcagat cattgcgctt tccgatcctg aagtggctca tcgcctggct
     6901 gcctttaagg aaggtctgaa agaaaaagtg ataaaagccg atgacgaact caaaaaaata
     6961 aaactccctt ataaagtgga ttaaaaaacc tacatcattt tttggtaaaa ttgccagtag
     7021 cgatcgggaa gttcgttgtt gcaagccgat tgataatcct gataagaaca aggcacaatg
     7081 atatggcggt aatatttggt ttgaagatat tcagggcaag gtacttgtag ccaccagcga
     7141 tcgctcttct tgcttttgta gaaaataatt tcttcttcca tattttctat tttgacaaaa
     7201 tacctgaaat gattttcgag tgaagggttt atacccggaa aatcgttcaa tctaccaaga
     7261 tagccttcca cgaaatacca gatcatctga gaaatgaggt aagccgttcg tccattgcag
     7321 tcatatttag ggttgtattc gaaaattccc agcgacgata ccttatcgct cattccggcg
     7381 aatcttgtaa tctggcaaat ctggtctccg aagaatccat tgggtgtagc ataagcgcag
     7441 ccaggtgcat cggaagctct gacagctgaa atgtcgatcg aaataaggtc ggcattacgt
     7501 acgaggggtt caatgttatc gatgttcggg ttgatctgcc ccaggcgata aacgtcgaag
     7561 agcatatttt tcatcaaagt aactgcggct gagtcaacaa aataggtttg gtatcccaga
     7621 tgagtataat tgaacagtac gctaggttga cgcaggaata gtttcgagag ccatgtggta
     7681 tggttgattt cttcgggagc ttctccaaga tcgaagaagg gatctacatt taccatgttg
     7741 atattgatgc cggttttttc atatcctaga tacatagcgt aagtcatgtc atgcgaacct
     7801 ccaagaacaa tgggaactat atcttgattt ctgagataag cataaacttc ggccaatcca
     7861 gcataagtat cttcgggggt attgccttgt ttcaaatttc ccaggtcggc catgggaaga
     7921 ggtgattttg gtggaaagag cctgtagaga taatttctga tgatatcggg tcccagctcg
     7981 caaccggcgt tgtgaggagc atttctggat tctggaatcc ctataagtgc aatatgaatg
     8041 tcggaaagat cagaaattcc tttttcagga gtatgtaatc tcaacagatt cccaaagtga
     8101 ggccttcctc tttttattga ttcgaattca gttttatcta atgggttgaa atatgcatcg
     8161 agttccatgg ttcgagagtt cttttctgaa agtattatac tgagataatt taaaaaaggt
     8221 tatagcttgg cgtaaaataa tccaaaaaag atcgaattat tttttacctc ttggttttgt
     8281 tttcttcttt gtaagagggg attcttccga ttcggctcga ctggcctttg attcgaggta
     8341 ttgtttttgc aattcgagac attgttggag ctcaagcgat tgagggtcga tttttttagg
     8401 aatgggaatg ttttcgtttc cgattttaat ataagggcca taacggccat tttggatgac
     8461 gacgtcaggc ctttcgggga aagtctttat gatttttgcg gcttcttttt gatttttttc
     8521 ttcgatgatt tgtatggctt tttcttggtt tatcatcaga gggtcttcat ctttaggaat
     8581 ggaatagaat gtgttactgt agagcagata aggtccataa cgaccagttg acactatgat
     8641 ttcctttccc atatagaatc ctacggtgcg gggtaattta aaaagagata gtgcttcttc
     8701 gagtgtgatg gtttctatgc tttgattttt cttcagaccg gcaaagcgag gtttctggcc
     8761 cgtaggatcc ttggtttctc caagttgcac tatcgaccca tatcttccta ttttggcata
     8821 gactttctta ccacttgctg ggtcgacgcc aagcaaacgt tcacctttga ctttttcttg
     8881 tgtttcgaaa gttttaacca cggtctcgtg aaagggccag taaaaacgct gaatcatctg
     8941 attccatttg agtttgccat cggctatctc gtcaaattcc ttttcaaccc ttgccgtaaa
     9001 ttgataatcc atgatattct cgaaattatg catcaagaat tgagtaacaa ggattccaat
     9061 gtcagtcgga gtgagttttc ctttttcgaa cccccatttc tcgattttcg attcttcttt
     9121 tatttttccg tttttcgaag taaggatgag ggcggttttt tcttgtccga catggtcttt
     9181 cttctcgacg tattctctct tttgtatcgt agagatgatg ggtgcatagg tcgaaggtcg
     9241 gccaattcca agttcttcga gtttttttac cagcgaagct tcggtatatc gtaatggagg
     9301 agtggtaaac ttttggttag ctgaaatgta tttttctttt atctcttctc caacgttgac
     9361 cgacggcaat attccagcat tcatttcttc cttttcttca tccgccgatt cgctgtatat
     9421 tttcaaaaat ccgtcgaaaa taacaacttc tcctttcgat aagaagtaat aacgatttgt
     9481 tttggatata gatatggcga tggttgtttt ttcgaggagg gcatcgctca tttgcgatgc
     9541 catagtgcgt ttccagatga gttcgtagag ttttagctgt gcttcgtttc ttccagcctg
     9601 atgtagcttc atgttggtag ggcgaatggc ctcgtgagcc tcctgagctc ctttggtacg
     9661 cgttgtgtat tgccgcgtct tgctgtattc tttcccatag agttttatta tttcgtcttt
     9721 tgccatcgat agagccagat cggaaagatt taccgagtcg gtacgcatat atgtgatcaa
     9781 accatcttcg taaagttgtt gagctatcat catggttcga gctaccgaaa agccaagttt
     9841 ccgagctgct tcctgttgca gggtagaagt ggtaaatgga ggtgcaggtg tgcgcttgcc
     9901 aggctttttc tctacatttt ttatcgtgta ggttgaaccc atgatgtggt tcagaaattc
     9961 gagagcagcc tctttggtcg gaaaacgatg atcgagctcg gccaacaact gtgctttgtc
    10021 atctttctta ttgagtaatt cgaacgaacc tgtgacacga aaataagtgg atggcttaaa
    10081 attctttatt tcgaattctt tttccacgat caatcgtacg gccaccgatt gtacacggcc
    10141 tgccgatagt gcgggtttga cctttttcca aagtaagggc gaaagctcat aacctactaa
    10201 acggtcgagg acgcgacgcg cttgttgggc atccacgagg ttgaaatcaa taaaacgagg
    10261 attttctatg gcttgctgga tagccttttt tgtgatttcg tgaaatacga tgcgttttgt
    10321 acgttccttc tccaattcta aaacttcata aagatgccag gcaatggctt ccccttcacg
    10381 gtcttcatag ttggctaacc aaatgatttc ggccttctga gcgacttttg aaagttcttc
    10441 gattatcttt tccttctctt ctgaaatttc atactgagga gcaaaatcgt tttgaatatc
    10501 aacgctgagt ttgtttttat cgaggtcgcg tatatgaccg aaactcgatt ttaccgtaaa
    10561 atcctttccc agaaaccctt ctatcgtctt ggccttggca ggagactcaa caataaccag
    10621 attttttgcc atgctttgaa gaatgtttga tatcgttgtt tgaaatgtct gcaaaagtaa
    10681 tattttgaaa ttattggttt tgtttttgta acctgctgta acactctgta ttgaatcttt
    10741 ctggatatta tggctaattt tgcattcgat ttcaatgcgt gatgttcgaa aaccgtttat
    10801 ttatcgaaac ttatggctgc cagatgaatt tctctgacag tgaaatagtt gcctcgattt
    10861 tgtcctctga aggttattct ctggtgaatg accctcatca agcaagcctt attcttatca
    10921 atacatgctc gataagggaa aaggccgaag aaagggtctg gaaaagatta agggagttga
    10981 aaagtttgaa gaagaagaat ccggagctta agattggaat aattggttgt atggccgaac
    11041 gcttgtatga ggagttgctt tttgccgggc atggtgtaga tttggtggca ggccccgacg
    11101 catatcgttt tttacccgaa ttattatcca atactcttca aggagcacaa gctgccaata
    11161 tacagctttc attcgaggaa acctatgaag atatagaacc cctccggctc gatccttcgg
    11221 gggtgagtgc ctttatttcc attatgcgag gttgcgagaa tttttgtgcc tactgcgtgg
    11281 ttcctcttac gcgcggaaaa gaacgaagcc gccaccctga aactattctg aatgaagcaa
    11341 ggaatctttt tcagagaggc tatccagaaa ttacattatt aggtcagaat gtcaattctt
    11401 attattttaa gaatgctcat ctaagcatga atttttccga tttgcttgct aaagtggcgg
    11461 aaattcatcc atctttgaga gtcagattca ccacctcgca tcctaaagat ctttccgata
    11521 aactcatttc tacgattgca tcttatccca atatttgcaa gtcggttcat ctggctgttc
    11581 aatcaggcag cgatgctatt ttgaaaagaa tgaaccggaa atatacacgc aatgagtttc
    11641 ttgatcgcgt acgttccatc cgtaaaatga ttcctgaatc gaccatttcg accgatatta
    11701 ttgcaggttt ttgtggtgaa acggaagaag atcacaggca aactctttca ttgatggagg
    11761 aagtaggttt cgattcggct tttatgtttc gatattcgga acggccagga accttagccg
    11821 cgagaaaata tcccgacgac gttccttctg aagtgaaggc aagacgcttg caggaaatca
    11881 tcgatcttca gcattggcat tcattacgaa gcaaccgtca ggatattgga aagactttcg
    11941 aagtgctggt agaaggttct tcgaaacgca gtcgagcgat gctttcagga cgaaattccc
    12001 agaacaagat ggtcgtattc gagccgaaaa aggctactag gggtcaatac gttcgggtac
    12061 gcataacggg ttgcactcag gctaccctca ttggcgaaga aatggaagat gatttataga
    12121 ggaaactctc aggcaaaaca aaacagctaa acctcgtgat gtaaggcttg gtgcagttag
    12181 cgcagaaagt acatgaaaaa tcagaagaaa gaatctttct ctttttacga tttcacaatc
    12241 aaggcaattc cattgaaacg gtccagatca tcgccatcta taaagaccac ttctccttgc
    12301 tgttcgatca ttttttcgat catttcatct ataatatctt caataaccga aggagcgtct
    12361 ttcattactt tttcgaccag agccacatgc ccgttgagta atactgccgg ttgatgaaaa
    12421 ccttttttca cgaacagggt tctgcctctg ccttgctgta tagcattcca aacctcggta
    12481 taatcggtca gtagattttg attattgatg gctttttcaa gtctcgagag ttgttttctg
    12541 gtttgatctt caaggttttt cttgaccagt tgccatgctt cagccacaat ttgatgggtt
    12601 ttggcatctt cgtgcaacaa gggcactctt cctattattt ttttcttatg ccgtgataat
    12661 tttaggaaga tattgtaatt gcgtatttca gaagctatta caacgggcaa atcgctgttg
    12721 cgaatcacct cccaaagtgc cttatccaca ttattgaaaa attcttcgat aagggattcc
    12781 tgaatctcat caggcgacaa tttagctttc tgctcattga acccctcgtt gtataggggg
    12841 aaaggttgaa ttttcaattc ttctccctga ttgttgatag ctttaatcaa tctggctttc
    12901 ttacggctca aggcgagtac gtaataagct gccgtttcgt gcatagcccg gtaaacatct
    12961 cttgtggcaa aagtcttatc gattaccaca cggttcttga cgctaaccgg cagagtgaga
    13021 tattgcccga attcgtggca aacaaaaaga attaaacttt tatcgttgcg agaaaaatca
    13081 atagaattga tggtgttttt caattgatga agaatggatt cggctgtttt gggatcacat
    13141 aacgacagaa tcctttcttc tgcttctctg gtgaggtttt taagagtcag catgtcttgc
    13201 tgaatggctg gccgggtttg atgtgtattc atgatgatgg taacacagac atcggcgtgt
    13261 gtgtttttaa tcttatcgag tgtatccata attcagcata ttaaaactct gtttgttttc
    13321 aattttatag ccttatgaga taaccgaaaa tctttgggtt tgttttatct ttgccttcaa
    13381 aggtacgaaa tttattcatt aaagtcaagt tttcaataag caaagttgat cctaaaattt
    13441 cggtatgaaa gatttggctg gtaatggcct ttatactgta tatttctaca tattggactt
    13501 ttaaaagtga gactgcaaat ttgggctgaa ggatttttga actttgaaga aagaagcaaa
    13561 aagattttgt ttactcgact ataaatcaat cgataaatgg caaagaaagg gagggacaag
    13621 cgtaacaagg ctgattcgct gagtactcat attctggcgg ttttttcagc cgatccgttt
    13681 cgtagttaca attacaagca ggtggctgct gcgatgggga taaaagacaa ggccacacgc
    13741 gatctaattt tttcgaccat gctcgaaatg ctgaaagaag gcatattgca ggaggtaaaa
    13801 agggggaagt tcagactcga tttatctcgc ttcgaccaga ttgtaccagc tagccgttat
    13861 attattggaa cggtagatat gaaacagact ggcaaagctt atatagttcc tcgggaacca
    13921 ggagaagata tatatatcag cccatcgcat acaggcaagg cttttcatgg tgataccgta
    13981 aaagtgtttc tttttccccg aaggcctggg cgtaaactcg aaggccaggt tgtcgaagtg
    14041 ctcaaacgcg ccaagacaca tttcgtgggg aaattacaac tgtccgacaa atatgccttt
    14101 gtcatccccg ataatccttc tatggcagtt gacttcttta tcccccgcga atatctcaat
    14161 aaggccaagg atggtgaaaa agtggtggtt gagttgatgg aatggcccga atatgcccgc
    14221 aatccctttg gccgtattat aaaagtttta ggtaaacctg gcgagcacca ggtcgaaatg
    14281 caaagtattc tggtggaatg ggatttccca ataggatttt cacgcgaagc cgaaaaggaa
    14341 gccaaagcca ttcccaaggc aatccctccg gaagaaatag agaaacgtcg tgatttcagg
    14401 aaggtgttta ccataactat cgatcctgcc gatgccaagg atttcgatga tgcactttcg
    14461 tttcgacagc tcgaaggcgg tctttatgag gtagggatac acattgctga tgtagcacat
    14521 tatgtaaggc caggaaccgc catagaccaa gaggccgaac gaagaggaac atctgtctat
    14581 cttgtggatc gcgtcattcc tatgttaccc gaaaaactgt cgaatttcgt ttgttcgctc
    14641 aggcccaacg aggataaact ctgcttctca gcagtattcg tgatggatcg tgaagcccat
    14701 ataacaagcc agtggtttgg gaaaagcatc atacgttccg attatcgttt caactatgat
    14761 gaggtacagc aaattattga aggaggccag catgccgtgc gcgatgagat tattgctctt
    14821 tataaacttt cggctaaatt gagagcagaa cgaatgaaaa aaggcacaat taatttcgaa
    14881 accaaggaag taaagtttga tctggatgac aaagggaaac ctctgggggt ttacatcaag
    14941 gagcaaaagg aagccaattg gctgatcgag gactttatgt tacttgctaa tcgccgagta
    15001 gcagaatggg ttggccatac caaaggtaaa accagatcca aaacttttgt atacagggtg
    15061 catgatgagc ctccagctga aaaaatcaac caatttgctc aatttgtaag caagttggga
    15121 tattccatta aactgggttc acgtgcttcg tttgctcgtt cgctaaaccg tctgtttacc
    15181 gaagtagctg gaaaagggga ggaaaacctc atcgaaacca ttgctgtgcg cactatggcc
    15241 aaggcttatt attcgaccga caatatcggt cattatggtt tggctttcga cttttacacc
    15301 cattttactt ctcctattcg tcgttatccc gatatgatgg tacatcgtct gttttgggat
    15361 tatctaaacg gagctggctc ggccgataag gaattctatg aagaacgttg catacatgcc
    15421 tcggagatgg agcgcaaggc actcgaagcc gaacgctcat cggtaaaact gaaacaggct
    15481 gaatttatga aagataaggt gggacaaata tttgatgcat tgatctcggg agtgagcaag
    15541 tatggtattt ttgcagagat tgtggacaac tactgcgaag gaatgatacg cctgcgcgac
    15601 ttgacggatg atttttatta cctcgatgaa gataattatc aggtgattgg acaaagatat
    15661 ggtgagactt atcgtttggg agaccccgta aaagtgatcg taaaaaacgt cgatattcag
    15721 aagaaacaga tcgattttaa actggtaaaa tcctgatcgt atttattatt cctgaggaac
    15781 ttctttgctg cgttcgtatt gggtgaagct aaatcgcata aatccgaagg aaagtgagag
    15841 atagggactt acattacgaa acaacacact aatgtcctga gtagccagtc cgatttccca
    15901 tcccggcaca agtttaatgg aaaatccgat tggaatatgg aaatcataat tgcctccggt
    15961 ttgaaatcct gccgagataa agaatttatt taatggacta gcctgcatac ccaaactgta
    16021 aacagggcgt tcttgagccc caggcacctt gttgagcggt atcataactt ccgaaccaat
    16081 gatgatatgt ggcactccgg tatatgctgc gcccaatcgc atttgagaag ggtatgatgc
    16141 ttttttatca ttcagtcctt cccattgcat caaatcatct tcaatgacaa tggcatcgaa
    16201 tacttcgttg atattttcgt ttcctaaacc aggagtttcg atttttcgaa tagtagcctc
    16261 gaacgaagcc gaatacacat tcttgttcca tgtcatcgtc ccaatgtcgt tgatagctaa
    16321 accaaagcgc cataaatttt tataacgacc cgataatcct atatcgaatg caaaaccgtg
    16381 tcccgaaggc cgtaattttc cattggtatc gagcgatgga gtaggttgtg ggtactcaaa
    16441 cttgaatacg ggcgacatgg acgagtatcc aaagaacttg tccttattca cgaaaatatc
    16501 cacccaggca aatcccatag tgtatttcat gcttaaccca ccatacaagc tccaatcctt
    16561 gatttccaca atttggcggc cataccccag aataaaatca cgcaaccagg ccgatgaaaa
    16621 ggatgatcct ctaaaaagtt ccgcaacggg ttttggattg gtcgaaatgc cagctatagt
    16681 caatccttgt tcgatcacct tgtagtcgaa ataatcactg ttaaaaccca gccaaagcag
    16741 ttcggccgca gttgaattca aatgcatgtt gaatgaacct ctttcgcgca tcgtaaaggc
    16801 aaaaccacca atatcagggt cttggtaaga aaccccaatg ccgttcatgc ctacgtcgaa
    16861 cgaaagacgt gaatcggaaa aacgtcgggc tgcctcctgc ttctcggcaa gcgtatattc
    16921 gtgatcttca tacaataaat tcaaatcact tttcgatacg gcatccgtat atcccgaaac
    16981 acttaatcct acaataccaa aatgccattt gtgattatcg gcattccatc ctaaattagc
    17041 cggattgatg cccaaacatt gataatcgct cacaacgatg accggcattc ccgaacccgt
    17101 tgccgtaaaa gcaccatatt ctgtttgaga aaaacttatt cctatgctaa agatacaggc
    17161 cattacaatg agaaacgccc tgcaaaatcg aattgtattt ttcatggtga tcgatatggt
    17221 taaaggatgg attggaatta ttattgaaca aatatatgaa cgtttcataa ttttaggtaa
    17281 tgtttctcga aaaatatttg aagaatacaa ccaaacattg tatcgaacta agatggaatg
    17341 gattcgggaa ccaaaacaaa gttatgaaat aagaagaaaa atggtgaaat gagcaaagaa
    17401 gctaaaaaaa aatatggcca gagtaaattc ctctccgatt cctcgttctg agatacagat
    17461 agattgaccg atcgtttcgt tacaagaact gagggagcta tttcaacctg ttcaggattt
    17521 tacctatact atctattact gccattgctt aataattttt tgatttttga taattcggtt
    17581 tccaccaagt agttgcagag tatatattcc tttcttcgaa gctgagaaat cgatggtcat
    17641 tcaatttcta tcgaacttat cgaattttag gagaatattt ctgcccggca tattataaaa
    17701 tggtatttcg tcaattcgca attggggtac atattcaata tactctggaa gctgaacatg
    17761 gaattgagaa atattttgac ttcatcagct ttattcaatt ccggtatctt catttccaaa
    17821 tcgcatggat ctccataatt gggataccaa gtagtatcat tgagtgtata acattttaat
    17881 cgggtaggga attcagtaaa aagaggacat tgctcaataa acccgccttt aaaacccatg
    17941 ccttctatta gaaaataaaa tttgacgaaa acggtctccc attaaacaat aaaccctgcg
    18001 ataaggtatc gagcacctga cttcgacaat gcgtcaccct aaaaattttt ccaaaacttt
    18061 tcatcaaaaa
//
LOCUS       DAOORU010000027        17825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_3851_length_17825_cov_6.680529, whole genome shotgun sequence.
ACCESSION   DAOORU010000027 DAOORU010000000
VERSION     DAOORU010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17825
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3851_length_17825_cov_6.680529"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /gene="pyrH"
                     /locus_tag="PLF20_03185"
     CDS             complement(<1..372)
                     /gene="pyrH"
                     /locus_tag="PLF20_03185"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004583568.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOX80045.1"
                     /translation="MTKYRRILLKLSGESLMGEQNYGIDPIKLADYASEIEEIVKMGV
                     QVAIVMGGGNIFRGLQGVSKGIDRVQGDYMGMLATIINCMALQAELEKNGIGTVLLGG
                     LEVRPIVMPMSRLQAIAYLEAG"
     gene            complement(390..3680)
                     /gene="secA"
                     /locus_tag="PLF20_03190"
     CDS             complement(390..3680)
                     /gene="secA"
                     /locus_tag="PLF20_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOX80046.1"
                     /translation="MLNFFKKFLGSKSERDIKAIMPIVNQIHVAYEEIQKLSNDELRA
                     KTKEFKTRIASYIAEEESKIAELKARIENEIDMDVEEKAEIYKQIDALSKLSYQKSQK
                     ILDDILPEAFAVMKDTARRFYENEQVIVTATQMDRDLAAHHENIRIEGDKAIYNKQWM
                     AGGNMITWDMVHYDVQLIGGIVLHQGKIAEMATGEGKTLVATLPVYLNALTGKGVHVV
                     TVNDYLAKRDSEWMGVLFQFHGLKVDCIDKHEPNSEARRLAYLADVTYGTNNEFGFDY
                     LRDNMTSSPDELVQREHIYSIVDEVDSVLIDDARTPLIISGPTPKGEDQLFDELKPPV
                     SRLYNLQRTLVTKILNEAKTLLSGNPTPDQEKKGGELLLRAYRGLPKHGPLIKFLSEP
                     GMKTLLQKTENFYLQEQAKNMHLIDDELYFVIDEKNNTIDLTEKGIDALTESYQDPEF
                     YLLPDVGSVIAEIEKMNIPESEKEERKSELMRDYAIKSERVHTVNQLLRAYALFEKDV
                     EYVIIDNKVKIVDEQTGRILEGRRYSDGLHQAIEAKENVKVEAATQTYATITLQNYFR
                     MYKKLAGMTGTAETEAGEFWDIYKLDVVVIPTNKPVIRTDREDMVYKTKREKYNSVIE
                     EIENLIKIGRPVLVGTTSVETSELLSRMLSLRKIKHNVLNAKQHAREAEIVAHAGLAG
                     MITIATNMAGRGTDIKLGPGVKEAGGLAIIGTERHESRRVDRQLRGRAGRQGDPGTSQ
                     FFVSLEDDLMRLFGSERIARIMDRLGIQEGEVIQHPMITRSIERAQRKVEENNFGIRK
                     RLLEYDDVMNAQREVIYSRRRNALHGERLSLDIRNMMEDVCEHLIEEYTDTRDYQGFR
                     MELLRIFGMDSPVNEDEFHSKSEKELHTLLFEYTYRRYKQRIQALAEKTYPVIKDVFE
                     NHSQFEYIAVPLTDGRKQINLIVNLKKAYQDKSREVVLTMEKTITLAVIDNAWKEHLR
                     EMDDLKQSVQNAVYEQKDPLLIYKFESFELFSKMLLRINKEITSFLMKASIPGAETQV
                     KEASLPKHTDRSRLKEQREDLLSQASPTQTSTKPAPVKVEKRVGRNDPCPCGSGKKYK
                     NCHGRIEN"
     gene            complement(3828..4049)
                     /locus_tag="PLF20_03195"
     CDS             complement(3828..4049)
                     /locus_tag="PLF20_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004362633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HOX80047.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDYAIRTGAPQEVIENLMEIE
                     DEGDVYESIEDIWPDYPTKEDFFFHEDEY"
     gene            complement(4129..4689)
                     /locus_tag="PLF20_03200"
     CDS             complement(4129..4689)
                     /locus_tag="PLF20_03200"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOX80048.1"
                     /translation="MGKGKKIYTHTGDQGETSLIGGQRVPKSHEKIEAYGTLDELNAF
                     TGLVIDLCNDMPTKEFLMLIQQKLFAVESLFAAASSEFQKDLLGVYENDIEMLEREID
                     RMNEVLPQLNSFILPAGHPSISSAHVARTVCRRAERCMVRAFSGSEQELAALKFINRL
                     SDFFFVLARKFAFDLNIPEIPWPANK"
     gene            complement(4809..5540)
                     /locus_tag="PLF20_03205"
     CDS             complement(4809..5540)
                     /locus_tag="PLF20_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005786909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX80049.1"
                     /translation="MMNEEIIRLINIKKYYKVGAEIVKALQSITMSVNRNEYLALMGA
                     SGSGKSTLMNIIGCLDTPTDGQYFLEGRDVSKMDDNQLAEIRNKKIGFVFQTFNLLPR
                     SNALDNVVLPLIYAGVSKEERIARGKEVLEQVHLADRMMHKPNELSGGQRQRVAIARA
                     LVNRPSIILADEPTGNLDSKTSLEILALFQEIHEAGNTIILVTHEEDIAKHAHRIVKL
                     RDGLIESDYPNLEIHRLPSTFASAL"
     gene            complement(5599..6372)
                     /locus_tag="PLF20_03210"
     CDS             complement(5599..6372)
                     /locus_tag="PLF20_03210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX80050.1"
                     /translation="MRFRAYIHYLLHAKSRYSVHSPFVFDLMENVFRDRHYHEYYYPA
                     ETVRKLMLHSDTTIQMMDYGMGGKKAKDYQVKVSQIARYSAKGKKNGRLLARISSYFS
                     PAVIVELGTSLGISALYLKAASPSSIVHTIEGSTEVAALARQNFQQAGFSDIHIHTGL
                     FEEVLPNLLKNGLKPDLVFIDGNHNKESTLNYFGGILPSLTPQSILIFDDIHWSKPME
                     ESWENVQQNPLVRVCIDLYWMGLVFVNTALSKQNYILRI"
     gene            6678..7181
                     /locus_tag="PLF20_03215"
     CDS             6678..7181
                     /locus_tag="PLF20_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80051.1"
                     /translation="MKNSNHQLEAIGLRLHKRIFVYEAILLILFIGSLFPYYIGKLWA
                     GTLILAITLILAIIYFFRGFRPCENNKDLMLYQKFTGLAEAMAILGIASRILYMQSDL
                     LFLGISGFIIVSLLIIHFIDVKKQNQKIRLERQDLIRLSILLVLTVIFMVWWPPLPRL
                     FYGEMIL"
     gene            complement(7215..8243)
                     /gene="pheS"
                     /locus_tag="PLF20_03220"
     CDS             complement(7215..8243)
                     /gene="pheS"
                     /locus_tag="PLF20_03220"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOX80052.1"
                     /translation="MKELVLRYMEEVDQATASSADEVEKFRIKFLGKKGILNELFAEF
                     NNIPPSEKRELGFLLNQLKEKVQQKVEQFRSILEMSKPFQKLDDLTFSADFYPMGSRH
                     PLSIVERRIIDIFARIGFTIAEGPEIEDDWHNFSALNFTPDHPARDMQDTFFIEKRAD
                     GNDVLLRTHTSSVQVRIMEKTKPPIRMIFPGRVFRNEAISARAHCIFHQVEGLYVDTN
                     VSFVDLKQTLLYFAREMYGESTRIRFRPSYFPFTEPSAEMDISCPLCKGEGCNICKHT
                     GWIEILGCGMVDPAVLENCDIHSETYTGFAFGLGIERITMGLYQIKDLRLFFENDTRF
                     LHQFKAAF"
     gene            complement(8303..8941)
                     /locus_tag="PLF20_03225"
     CDS             complement(8303..8941)
                     /locus_tag="PLF20_03225"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX80053.1"
                     /translation="MRKTFLLFILLMSIGIGSLMAQSKEKVTYFIIKTDYGQMKGFLY
                     NETPKHRDNFVKLAKQGWYNGSPFHRVIKDFMIQGGQNADGRVDPGYRIPAEINSKFI
                     HKKGALAAARMGDQVNPAKESSGSQFYLVQGTPLDEKTIQAYAARNGMKYTPQQLEIY
                     KTLGGTPHLDGAYTVFGEITEGLDVIDKIASVKTLQGDKPAKDLKMTIRIIE"
     gene            complement(9020..9607)
                     /locus_tag="PLF20_03230"
     CDS             complement(9020..9607)
                     /locus_tag="PLF20_03230"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOX80054.1"
                     /translation="MFLELPLVKKQPSGWIEVITGSMFSGKTEELIRRLRRAHFAKQN
                     VEIYKPDIDTRYHEQDVVSHDANAIPCTPVVSSQQILLYVGDVDVIGIDEAQFFDMEI
                     VNVCQQLADEGKRIIVAGLDMDYKGQPFGPLPALMACAEFVTKVHAICMDCGNLAHYS
                     FRTVPDKKLVLLGETESYTPLCRNCFNRRMKNQNQ"
     gene            9680..10465
                     /locus_tag="PLF20_03235"
     CDS             9680..10465
                     /locus_tag="PLF20_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80055.1"
                     /translation="MTIAEKNLIIDEIFDMIEVLKEQLNSFKTDEERIPQIELDLCME
                     NVRRLYEKLKLLDSLNMGTRPKSQPAPSVSKQPASIRIRFEGVEKNIPEKREPPSPTE
                     TSISHPSSTNFKEPEPFAKPQPSEEPAKKQHSKPKTVPFTPDLFSQEIPIIADKLAEE
                     KPTLIDKLAVHTDDKSIVDNLTNRPLADLRAAIGINEKFLFVNELFGGSLQEYNQAVQ
                     ALNQAGNLDNALEIFNSYQLRYRWNPKLPGVNKLKELIQRRYL"
     gene            complement(10468..11028)
                     /locus_tag="PLF20_03240"
     CDS             complement(10468..11028)
                     /locus_tag="PLF20_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOX80056.1"
                     /translation="MNYFDIIVGLILIFFGIRGFLKGFLIGVASFAGLLAGLYAAIHF
                     SNYMGNVLSQWLKFSEQSIKIFSFLITFAVVIFLVHILARVLEKVVSASGLSILNKLG
                     GLILGAAKALLIVSAFFYLFTLLGGLKLMDDSIKEKSLFYKPVSKVAKFIAGEVLEQL
                     EENKKSKQIEPEEVNAIWTGFKMNAQ"
     gene            complement(11036..11788)
                     /locus_tag="PLF20_03245"
     CDS             complement(11036..11788)
                     /locus_tag="PLF20_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX80057.1"
                     /translation="MITVSHISKSFGKTKVLSDISAQFETGRVHQVIGQSGSGKTVLM
                     KCLVGLLEVDEGEVLYDGRNFSQMTANQRKEIRKEMGMLFQGGALFDSMNVAENVAFP
                     MLMHSNLTTAEMKKRVDFCLERVNLAHSHKLIPSELSGGMTKRVAIARAIALNPHYLF
                     CDEPNSGLDPQTANMIDNLIAEITHEYNMITIVNTHDMNSVLEIGEEVNFIYKGKLWW
                     KGDRNSILNSNNAELNQFVFATGLMRRLKPCQ"
     gene            complement(11785..12534)
                     /locus_tag="PLF20_03250"
     CDS             complement(11785..12534)
                     /locus_tag="PLF20_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX80058.1"
                     /translation="MRFIRIIEDIGHYVHLMKRVLTKPDKSLVLKRQFFVNVQSLGFD
                     SIGIVAIISLFIGAVATIQVAYNIDNPLIPLSLIGFTARQIIILEFSPTVVSLILAGK
                     IGSRIASEIGTMRVTQQIDALDVMGINSANYLILPKILASVFFNPILIVMSIAISLVG
                     GWIVSLTTSLLTPHDYIEGIRLFFDGFTVFYALIKTVVFAFIIASVSGFHGYRITGGA
                     HEVGQASTRAVVDSSIAIIISNLILTQLLLT"
     gene            complement(13187..14140)
                     /locus_tag="PLF20_03255"
     CDS             complement(13187..14140)
                     /locus_tag="PLF20_03255"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HOX80059.1"
                     /translation="MHVCEILSKTNKTLFSFEILPPLKGGSFNDIFNSIEPLLEFKPA
                     YINVTYHQEEVVYKDRGNGLVEKMVVRKRPGTVGIAAALQYTCNIQVVPHIICGGFTR
                     EETENALIDLSFLGINNVLVVRGDPDRSQKTFIPEPDGHIHSLDLVKQIVDLNHGKYL
                     EEDLQNSNPTNFCIGVAGYPEKHPEAPNKASDLRYLKMKIDAGASYIVTQMFFDNRKY
                     FDFVDNCHEIGINVPIIPGLKPVAICQHLHLLPRTFGIDLPDDLAKELEKCKTSQEAR
                     NLGIEWTIHQTRELIAAHVPAVHFYTMGRSDNIYRIASACF"
     gene            complement(14150..17608)
                     /gene="metH"
                     /locus_tag="PLF20_03260"
     CDS             complement(14150..17608)
                     /gene="metH"
                     /locus_tag="PLF20_03260"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF007024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HOX80060.1"
                     /translation="MSQADYKLEHLVYQMNYEAARLAIEVARQYSTPTKPRFVAGSIG
                     PTNKTLSLSPDINNPANRAISFEQMEEAYVTQVEALLDGGVDILLIETIFDTLNAKAA
                     LLACKRVLQAKGKDIVMTARGLKRRFPIMVSGTISDASGRTLSGQTPEAFLISLSHAE
                     LLSIGFNCSMGALQLRPYIEEIASKAPFYISVYPNAGLPNQFGEYEQSPDEMAHDLAP
                     LLHASKVNIIGGCCGTTPEHIRKLVEMVNLSQPHLPSPRHEALMLSGLDPLVQFPGSN
                     FINIGERTNVSGSKKFARLIREERFEEALQVARQQVENGAQILDINMDDALLDSERCM
                     KIFLHHLMSDPEIARIPLMIDSSKWSVIDTGLQCSQGKCIVNSISLKEGEKVFIQHAR
                     TILDYGAAVVVMAFDENGQADTFERRISICQRAFRLLTHKVGFHTSDIILDANVLAIA
                     TGMSEHNQYAVEFIRAVKWIKENLHGVRTSGGISNLSFSFRGNDKIRQAIHSVFLYHA
                     IQAGLDMGIVNAGALPVYEEIPEPLLTLVEDVILNRRKDATSRLVMYANQYGKKAEID
                     SLITHNWRSDSLEKRLEHALIKGISDFLEEDMKEALETYPSALQIIEGPLMEGMNLIG
                     ELFGSGKMFLPQVIRAARVMKKAVDFLTPYLEKEKETNGNNQTSAGKILLATVKGDVH
                     DIGKNIVKVVLSCNNYEIIDLGVMVPAKTILETAIENKVDMIGLSGLITPSLEEMCLV
                     AEEMEIRGMTQPLLIGGATTSELHTALRIAPKRSYPVIHVKDASLAVNVANQLINHSQ
                     QQKYWADIQLKYENLREKYQATKNVSQTYITINQARSNRLKMDWDKEPICQPAFIGTK
                     YFHEYDLSEISPFIDWTFFFHAWRLTGKYPSLFDDPVKGEEANKLYADAQEMLYKIIQ
                     NKWLQANAAFFVLPANSLGDSIEVYHPDGRVWKILHFLRNQEKKPDGIPNLCLSDFIA
                     PKESQRQDYLGAFAVTTGIGIETQLDRFDKNHDDYSGIMLKILANRLAEAFAELLHKR
                     VRREFWAYAPDENLSMEDILKEKYQGIRPAPGYPACPDHSGKVTLFEMLDAEKQIGIS
                     LTSHYAMYPAASVCGYYFSHLQAKYFNVGKINPDQIQDYALRKQLSIREVEKLLRTHL
                     NYEPLT"
     gene            complement(17563..>17825)
                     /locus_tag="PLF20_03265"
     CDS             complement(17563..>17825)
                     /locus_tag="PLF20_03265"
                     /inference="COORDINATES: protein motif:HMM:NF019158.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="virulence factor SrfB"
                     /protein_id="HOX80061.1"
                     /translation="TSCQLVFLYSLLTKKIKNNPSVIADILLPNKKMKIASIDIGAGT
                     SDLVINEYVTNLDGSNISLKPTPSMQMPSHKPIINSNILFTR"
BASE COUNT         4981 a         3662 c         3723 g         5459 t
ORIGIN      
        1 acctgcttcg aggtaagcaa tggcttgaag acggctcatg ggcataacga tgggtctcac
       61 ttcgagcccc cctaataata ctgttccaat gccattcttt tcgagttcgg cttgcaaagc
      121 catacaattg atgatagtag ccaacatacc catatagtct ccctgaacac ggtctattcc
      181 ctttgagact ccctgcaatc ccctgaagat atttcctccg cccataacta tggctacctg
      241 aacccccatt tttactattt cttcaatttc agaagcataa tcggctaact tgatgggatc
      301 aattccataa ttctgttctc ccataagtga ttctccacta agtttcagta atattcgcct
      361 atatttggtc atttatcgat ttgtttgttt taattctcaa ttctaccgtg gcagttctta
      421 tattttttac cacttccaca agggcagggg tcgtttcgtc caacgcgttt ttctactttc
      481 accggtgcag gtttcgttga ggtttgggtt ggactggctt gcgataacaa atcttcgcgt
      541 tgttccttca accggcttct atcggtatgt ttaggaagac tggcttcttt tacttgtgtt
      601 tcggcacctg gaatacttgc tttcataagg aaggaagtaa tttctttgtt gattctgagc
      661 agcatcttgc taaaaagttc gaacgactcg aatttataga taagcaaagg gtctttttgt
      721 tcgtaaacgg cattctgaac cgattgtttg agatcgtcca tttcgcgtag atgttctttc
      781 catgcattat caattactgc cagggtaata gttttttcca tggtaagtac tacttcacgg
      841 cttttatcct gataggcttt ttttaaattg actatcagat tgatttgttt gcggccatcg
      901 gtaaggggaa cggctatgta ttcgaattgg ctatggtttt cgaaaacatc cttgatgacg
      961 ggataggttt tttcggccaa agcctgaatt ctttgtttgt agcgacgata tgtgtattcg
     1021 aagagaaggg tatgcagttc tttttctgat ttgctatgaa attcatcttc gtttaccggg
     1081 ctatccatgc cgaatattct aaggagttcc atacggaagc cttgatagtc gcgggtgtcg
     1141 gtatattctt ctatgagatg ttcacataca tcttccatca tgttgcggat atcgagtgaa
     1201 aggcgttcgc catggagggc attgcggcgt ctgctgtaga tcacttctct ctgggcgttc
     1261 attacgtcgt cgtattctag cagacgttta cgaattccga agttattttc ctctacttta
     1321 cgttgtgcac gttcgatgga gcgggtaatc ataggatgtt gaatcacttc tccttcctgg
     1381 ataccaaggc ggtccatgat acgcgcaatg cgttccgagc cgaagaggcg catcaggtcg
     1441 tcttcgagcg agacgaagaa ttgggaggtg ccagggtcac cctgacggcc agcccgtccc
     1501 cgaagctgac ggtcaacacg ccttgattcg tgacgttccg taccaatgat tgctaaacct
     1561 cctgcttcct ttacgcccgg tcctaatttt atatccgtac cacgaccagc catgtttgtt
     1621 gctatggtaa tcattccggc caaaccggca tgtgctacga tttcggcttc tcgggcatgt
     1681 tgcttggcat taagtacatt atgctttatt ttacgcaacg ataacatcct gctaagcaat
     1741 tcggaagttt ctaccgaagt agtacccacc aaaacaggcc gtcctatctt gattaaattc
     1801 tcgatctctt cgatgactga gttatatttt tcacgtttgg tcttgtaaac catgtcttcc
     1861 ctatcagtac ggatgacagg cttatttgtt ggaatgacta ctacatcgag tttgtagatg
     1921 tcccagaatt cgccggcctc cgtttcggct gtacccgtca tacccgcaag tttcttgtac
     1981 atgcggaagt aattctgaag agtgatggta gcgtaggttt gagtagctgc ttccactttc
     2041 acattttctt tggcttcgat agcttgatga agaccgtcgg aataacgtcg gccttcgagg
     2101 atacgtcccg tttgctcgtc aacgattttt actttattat cgatgataac atattccacg
     2161 tctttttcga aaagtgcata agctctcaat aattgattta ctgtatgaac gcgttctgac
     2221 ttgatggcgt agtccctcat aagttcgctc ttgcgctctt ctttttccga ttctggaatg
     2281 ttcattttct cgatttctgc aattaccgag cccacgtcgg gaaggaggta gaattcaggg
     2341 tcctggtaag attcagtcaa agcgtcgatt cccttttcag taaggtcgat ggtattgttt
     2401 ttttcatcga ttacaaaata gagctcatcg tctatgaggt gcatattctt ggcttgttcc
     2461 tgaaggtaga agttttcggt tttctgcagt aaggttttca tgccaggttc actcagaaat
     2521 ttgattagcg ggccgtgttt ggggaggccg cgataagcac gcaacaaaag ttcgcctcct
     2581 tttttttcct gatcaggggt ggggtttccc gagagtaatg tttttgcttc gttgaggatt
     2641 ttggttacaa gggtacgctg caagttgtag agacggctaa cgggtggttt tagttcgtcg
     2701 aagagctggt cttcgccttt gggggtaggg cctgaaatga tgagaggggt gcgtgcgtcg
     2761 tcgattaaga cggaatccac ttcatccact atggaataga tatgttcgcg ctgcacgagt
     2821 tcgtcgggtg aggaagtcat gttatcgcgg agatagtcga agccaaattc attgttggta
     2881 ccataggtaa catcggctaa gtaagccaaa cgacgagctt cgctattggg ttcatgtttg
     2941 tcgatacagt ccacttttaa accatggaat tggaaaagca cacccatcca ttccgaatcg
     3001 cgtttggcca aataatcatt taccgtaacc acatgaaccc ccttacctgt aagcgcattc
     3061 agatagaccg gcagggtagc tacaagggtt tttccttcgc cagtagccat ttcggcaatc
     3121 tttccctgat gcaaaactat acctccgatg agttgtacat cgtaatgcac catgtcccaa
     3181 gtgatcatgt ttccgccggc catccattgc ttgttgtaga tggccttgtc tccctcgatt
     3241 cgaatatttt cgtgatgagc tgccagatcg cgatccattt gtgtggcagt gacgattacc
     3301 tgttcgtttt cgtagaatct tctggcggta tctttcatca cagcaaaggc ttcgggcaag
     3361 atatcatcca gaattttctg gcttttttga taggaaagtt tcgaaagagc atctatttgc
     3421 ttataaatct cggctttctc ttctacatcc atgtcaattt cgttctctat gcgagccttc
     3481 agttctgcaa ttttcgattc ttcttcggca atatagctgg cgatcctggt tttaaattct
     3541 tttgtcttgg ctctcagttc atcgttagat aatttttgaa tttcttcata agccacatga
     3601 atctgattta cgataggcat tatggcttta atatcgcgct cggatttaga accgaggaat
     3661 ttcttgaaga agtttaacat ttttatttag ttgataataa ttcaatggat tataaaaaga
     3721 taatttgcaa agttagccaa aatcatctac acatagaggg gaatgcaggc gttatgaaga
     3781 tgaaaaaagc agggtagaac cctgccatgt agatgggtcg aaaaaaatta gtactcgtct
     3841 tcatgaaaga aaaagtcttc tttagtaggg tagtcaggcc aaatatcttc tatgctttca
     3901 taaacatcgc cttcgtcctc aatttccatg agattctcga taacctcttg tggagcgcct
     3961 gtccgaatag cgtaatcaat caattcgtcc ttggtggcag gccagggagc atcctctaat
     4021 ttcgatgcca gttcaagcgt ccagtacata ttgaaataat ttatttataa ctaaaaaagt
     4081 ttgcaaaaat ataacaaact ttgtttctat aagtaaaatt cgaaaaaatt atttattagc
     4141 aggccaggga atttcgggta tattcagatc gaaagcaaat ttgcgggcaa gtacgaaaaa
     4201 gaaatccgat aatctattga tgaacttcaa agccgcgagc tcctgctcgc tgcccgaaaa
     4261 cgctcttacc atgcaacgtt cggctcggcg acaaactgta cgtgccacat gggcagacga
     4321 aatcgaagga tgccctgcgg gtagaataaa tgaattcaat tgaggcaaaa cttcattcat
     4381 gcggtcgatt tctctttcaa gcatttcgat atcattttcg taaaccccga gaagatcctt
     4441 ttgaaattct gatgaagctg cggcaaaaag actttctact gcaaataatt tctgctggat
     4501 tagcatcaaa aattcttttg taggcatatc attacataga tcgatgacga gtccagtaaa
     4561 ggcatttagc tcgtcgagtg taccgtaagc ttcgattttt tcgtgcgatt taggtactcg
     4621 ctgtccgccg atgagagatg tttcaccttg gtcgcctgta tgtgtataaa tttttttccc
     4681 ttttcccatg gaataatttt atttaaggca atcgagaaat ctttcgatgt cagattattt
     4741 catgcatgtt tcatttgaaa aacgagtctt cagttcattt attagaatta taatctacga
     4801 tatagaaact ataaagccga ggcgaaggta gatggcaggc ggtgtatttc gagattggga
     4861 taatcggatt cgatgagtcc atcgcgcagt tttacgatac gatgggcgtg tttggcaata
     4921 tcctcttcgt gggtaaccaa aattatcgta ttgcctgctt cgtgtatttc ctggaaaaga
     4981 gccaaaattt cgagagaggt tttcgaatcg aggtttccgg tgggttcgtc ggcaagaata
     5041 atagaaggcc tgttgaccag agctctggcg atggctaccc tttgtcgttg tcctcccgaa
     5101 agctcgttgg gtttatgcat catacggtcg gccaaatgta cctgttcgag cacttccttg
     5161 ccgcgggcaa ttcgttcttc tttcgaaacg cctgcataaa tcaaaggcaa tacaacatta
     5221 tcgagtgcgt tgctacgagg caaaagattg aaagtttgaa acacaaaacc aatcttcttg
     5281 tttctgattt cagcaagctg gttgtcgtcc atcttgctta catcgcggcc ttccagaaaa
     5341 tactgaccat cggttggcgt atcgaggcat ccgattatgt tcatcaaggt tgatttacca
     5401 gaacccgaag cacccatcaa ggctaaatat tcgttcctgt tcacactcat ggtgatggat
     5461 tgcaatgcct taactatctc ggctcctacc ttgtagtatt tctttatatt gattagtctt
     5521 ataatttctt cgttcatcat ttaagcggat taagctacaa aagtaaccat tatcattgtg
     5581 aaagaaacaa tcgaaaaact atatccggag aatataattt tgtttactaa gagctgtatt
     5641 aacaaaaacc aatcccatcc aataaaggtc gatacaaact ctaaccagag gattttgttg
     5701 gacgttttcc caggattctt ccataggttt cgaccagtgg atatcatcga aaattagaat
     5761 gctttgtgga gtgagcgaag gcaaaattcc accgaaataa ttcagggttg attctttgtt
     5821 gtgattcccg tcaatgaata cgaggtcggg tttcaatccg tttttcaata agttgggtaa
     5881 gacttcttcg aataatcctg tatgaatgtg aatatcggag aacccagcct gttgaaaatt
     5941 ctgccgtgca agtgccgcca cttcggtact tccttctatg gtatgaacaa tggatgatgg
     6001 agaggcagcc ttcagatata aagcactaat tcccagcgaa gtgcctaatt ccacgatcac
     6061 agccggagaa aaatatgagg atatccgtgc caataaacga ccattctttt tcccttttgc
     6121 agaataacga gctatttggc taacttttac ctgatagtcc ttggctttct tgccccccat
     6181 tccataatcc atcatctgaa tggttgtatc ggaatgcaac atgagtttac gaactgtttc
     6241 ggctggatag taatattcgt ggtaatgtct gtctctgaat acattctcca ttaaatcgaa
     6301 gacaaagggc gaatgcaccg aataacgtga ctttgcatga agcaaataat gaatgtatgc
     6361 tctgaatctc acgacaaaaa tttgatgaag caaaactaaa aagcttgaat gaattattaa
     6421 tttctttctc caggtcaatc gtccaatttc aatattacat cgagatatgt catttaattt
     6481 atgactcgat gaaaagcaaa agataatttt ctcatttggc gaattttttc atacacagat
     6541 aggtttattt aattcgcgat tagaattatt ggataaaaag caaggcaaaa acgggatgtt
     6601 attattatag gtgattttga atgttttggg taaaatttaa atcaaatgag taatttcgcc
     6661 aattgaaaat tttgtttatg aaaaattcga atcatcagct cgaggcgata gggctcaggt
     6721 tacacaaaag aatttttgtt tacgaagcca tactgctcat tttattcata ggttcattat
     6781 tcccttatta tattgggaaa ttatgggcag gaactttgat attagccatt acgcttattc
     6841 ttgccattat ttattttttc agaggattca gaccctgtga gaataataaa gacctcatgt
     6901 tatatcaaaa atttacgggt ttggccgagg ctatggcaat tcttggaatc gcatcgagaa
     6961 ttctatatat gcaaagtgat ttactttttt taggtatttc ggggttcatt atagtcagtt
     7021 tgcttataat tcactttatt gatgtaaaaa agcaaaacca gaagatacga ctcgagcgcc
     7081 aggatttgat acggttgagt attttattgg tgctcaccgt aatttttatg gtgtggtggc
     7141 cgccattgcc tcgacttttt tacggtgaaa tgattttata gaaaagcaga aagatttcga
     7201 ggctgacttg taacctaaaa agccgctttg aattggtgta aaaaacgcgt atcattttcg
     7261 aagaacaggc ggaggtcttt aatttgatag aggcccatgg taatgcgttc gatgccaagg
     7321 ccaaaagcaa atcccgtgta agtttcgctg tggatgtcgc aattttcaag aactgccgga
     7381 tcaaccatgc cacagcccag aatttcgatc catccggtat gtttacaaat attgcatcct
     7441 tcgcctttgc aaagagggca gctaatatcc atttcggccg atggctcggt aaaagggaag
     7501 taggaaggac gaaatctgat gcgggtcgat tcgccgtaca tttcacgggc aaaatatagc
     7561 agggtttgtt tgagatcgac gaaagaaaca ttcgtatcta catacaatcc ttcgacttga
     7621 tgaaatatgc aatgagcacg agccgaaatg gcctcgtttc gaaaaactct gccaggaaaa
     7681 atcattcgaa tgggaggttt tgttttttcc atgattctaa cctgtaccga agacgtatgg
     7741 gttcgtaaga ggacatcgtt gccatcggct cttttttcga tgaagaaagt gtcttgcata
     7801 tcgcgtgccg gatgatcagg ggtaaaatta agagccgaaa aattatgcca gtcgtcttca
     7861 atttcagggc cttcggctat agtaaatcct atgcgggcaa aaatgtcgat gattcgtctt
     7921 tcgacaatag ataatggatg acggcttccc attggataaa aatctgccga aaatgtgagg
     7981 tcgtcaagtt tttggaaggg cttactcatt tcgaggatag aacgaaactg ttcgactttt
     8041 tgctgaactt tttccttcag ttgattcaat aaaaaaccta attcgcgttt ctcggaggga
     8101 ggaatattat tgaattcggc aaataattca ttcagaattc ccttttttcc taaaaatttg
     8161 atacggaact tctccacttc atcggcagaa ctggctgtag cctgatctac ctcctccata
     8221 tatctcagaa caagttcttt catagggaga atcattgaat ttattggcta aaagaatgaa
     8281 agtacgaata agaaattgaa tattattcga tgatacgaat ggtcattttc aagtctttgg
     8341 cgggtttatc gccctgaagg gtttttacac tggcgatttt atcgatgaca tcgaggcctt
     8401 cagtaatttc accaaacaca gtataagcac cgtccaaatg tggcgtgcct ccaagggttt
     8461 tatagatttc gagttgttga ggagtgtatt tcattccatt acgtgccgca tacgcctgaa
     8521 tggttttttc gtctaaaggc gtaccttgca caagataaaa ctgagaaccc gaagattctt
     8581 tggctggatt gacttgatcg cccatgcgag cagctgccaa tgcacctttt ttatgaatga
     8641 atttagaatt gatttcggct ggaatcctat agccggggtc gactcttcca tcagcgttct
     8701 gacctccctg tatcatgaaa tctttgatta cacgatgaaa aggcgaacca ttataccacc
     8761 cttgtttcgc caattttaca aaattatccc ggtgtttggg ggtttcatta tataaaaacc
     8821 ctttcatctg gccataatcg gttttaataa taaagtaagt aaccttttct ttggattgag
     8881 ccatcaaaga acctattcct atactcatca gcagaataaa taagagaaaa gtttttctca
     8941 tttttttatt ttttttatca ttaaacttag tgctggcaaa ggtaagaaaa attgcccgag
     9001 cattctgaat tgttaagaat cattggtttt ggtttttcat tcttcgattg aaacagttgc
     9061 ggcacagggg agtgtagctt tcagtttcac ccagtaggac gagttttttg tcaggaacgg
     9121 ttctgaagga ataatgggcc aaatttccgc aatccatgca aatggcatgc accttggtta
     9181 caaattcggc gcaggccatc agggcaggca acggaccaaa tggttgcccc ttatagtcca
     9241 tatccaaacc tgccacaata atcctcttgc cttcgtcggc aagctgttgg catacattta
     9301 caatttccat atcgaaaaac tgggcttcgt ctatgccaat tacatccaca tcgcctacat
     9361 acagcagaat ttgctgagac gaaacaaccg gtgtgcatgg aatagcatta gcatcgtgag
     9421 atacgacatc ctgctcatga taacgagtat cgatatctgg tttatatatc tcgacatttt
     9481 gtttggcaaa atgcgcccgt cttagacgac ggatcaactc ttcagtcttt ccagaaaaca
     9541 tggaaccagt gatcacttcg atccatcctg aaggttgttt ttttacaaga ggtaattcta
     9601 agaacatttc gattaaaaat aaaatttctt gagtttttcg tgtaatttta cctacaaatt
     9661 tatttcacct ttcatgaata tgacgatagc agaaaagaat ctaattatcg atgaaatttt
     9721 cgacatgata gaagtgctta aggaacaatt gaattcattc aaaacggatg aagaaagaat
     9781 tcctcaaatc gagctcgatc tttgcatgga aaacgtacgc cggctttatg aaaaattaaa
     9841 attgctcgat agcctcaata tgggcacaag gccaaaatca caacccgcac cctccgtatc
     9901 gaagcaaccg gcttccattc gtatccggtt cgaaggagta gaaaaaaata ttcctgagaa
     9961 gagggaacca ccttctccta ctgaaaccag tatatcgcat ccctcatcta caaattttaa
    10021 agaacccgaa cctttcgcca aacctcaacc ttccgaagaa cccgccaaaa aacagcattc
    10081 caagccaaaa accgtgcctt ttactcctga tctgttcagt caggaaatac ccattattgc
    10141 cgataaactt gccgaagaaa aaccgacatt gatcgacaag cttgctgtcc ataccgacga
    10201 caagagtata gtagacaatc tgacaaaccg gcctttggct gatcttcgag ctgctatcgg
    10261 tatcaatgag aaattcttgt ttgtaaacga gcttttcgga gggagtttgc aggaatacaa
    10321 tcaagccgtt caggctttga atcaggcagg aaatctcgat aatgcactcg aaatatttaa
    10381 ttcgtatcaa ttacgttacc gttggaatcc aaaacttccg ggagtcaata aactaaaaga
    10441 actcattcag cgaagatatt tataaaacta ttgagcattc attttgaatc ctgtccagat
    10501 agcattcacc tcctctggtt cgatttgttt tgattttttg ttttcttcca actgttccaa
    10561 tacttcacct gcaataaatt ttgctacctt tgaaaccggc ttatagaaaa gacttttttc
    10621 ttttatactg tcatccatca gtttaagccc tcccaataga gtaaataaat aaaaaaatgc
    10681 acttacaata agcaaggctt tggctgctcc caagatgagc ccccccagtt tattcaaaat
    10741 gcttaaccct gatgccgaaa ctaccttttc aagaaccctt gccagaatgt gaaccagaaa
    10801 aataaccaca gcaaaggtga tcaagaatga aaatatcttt attgactgct cggagaactt
    10861 taaccactga gaaagtacat tgcccatgta attggagaaa tgtatggctg catataagcc
    10921 agcaagaaga cctgcaaacg aggccacgcc aattagaaat cctttaagga atcctcgtat
    10981 tccgaagaat attaaaatca agcccactat aatatcgaaa tagttcatgg taaactcatt
    11041 gacagggctt tagacgtcgc atcagtccag tggcaaaaac aaattgattc aattccgcgt
    11101 tatttgaatt cagaatggag ttcctatcgc ctttccacca taatttacct ttgtagataa
    11161 aatttacctc ttcgccaatt tcgagaaccg aattcatatc atgagtattg acgatagtta
    11221 tcatgttgta ttcatgtgtg atctcagcta tcagattatc tatcatgttg gcggtttggg
    11281 ggtcgagacc cgagttaggt tcatcgcaaa aaaggtaatg agggttaagg gctatagcgc
    11341 gcgcaatggc cactcttttg gtcattcctc cacttagttc ggatggtatt aatttatgag
    11401 aatgagcgag attgactcgt tccagacaga aatccactct ctttttcatt tcggcagtcg
    11461 tcagattgga atgcataagc attgggaagg ccacattttc ggctacattc atcgaatcaa
    11521 acagggctcc accctggaac aacatcccca tttccttcct gatttctttt ctctggtttg
    11581 ctgtcatttg cgaaaaattt cttccatcat acaatacttc accttcatct acttcgagca
    11641 agcccactaa gcacttcatc aaaaccgtct tgcccgatcc actctggcca ataacctgat
    11701 gaacccgccc ggtttcgaac tgggcagaaa tatcactcaa taccttggtt ttaccaaatg
    11761 atttcgaaat atgagagact gtgatcatgt aaggagaagt tgggtaagaa tcaaattgga
    11821 aataataatg gcaatgctcg aatcaactac tgctctcgta cttgcctgcc ccacctcatg
    11881 tgcacctcct gtgattctgt aaccgtgaaa acccgaaaca ctcgcaatga taaaggcaaa
    11941 taccactgtc ttaattaatg cataaaaaac tgtaaaacca tcgaaaaaaa gacgtattcc
    12001 ttctatgtaa tcgtgaggag taagaagaga agtagtaaga gatacaatcc agccaccaac
    12061 cagacttatg gctatgctca tgacaatgag gataggattg aaaaaaacgc tggccagtat
    12121 cttaggcaaa atcaaataat tggctgagtt gatacccatt acatcgagcg catcgatttg
    12181 ttgtgtaacc cgcatggtgc caatttcgga agcaattcgt gaaccaattt tcccggccag
    12241 gataagactg actacagtgg gagaaaactc taaaataatg atttgccgtg ccgtgaaacc
    12301 gatcaatgat aatggaatca atggattatc gatattataa gctacctgaa tagtggctac
    12361 tgctccgata aacagactga tgatagctac tataccaata gaatcgaatc ctaatgattg
    12421 aacattgaca aagaactgac gcttaagtac caatgattta tcgggtttgg tgagaaccct
    12481 cttcatcaag tgaacataat gccctatgtc ttcgatgatt ctgatgaatc gcatgccacc
    12541 cagctttggt gcgaaattac gctttttctc gattgtcgga atgcaataat ttcgtataaa
    12601 aatgaaaatg ggggatggtg aatggagatt agtggtgtgg tgatcagggg acggggagag
    12661 gggggactgg tggataatga ttagtgatta gaatttggaa ttggaaacgg aggaagggag
    12721 actgcgggac gatgagaacg ggagattggt ggatagtgga tagtggatag tgaatggaaa
    12781 ttttgagagt gggggacgaa tagacgaggg gactggaatt tgtaatttgg agattaaagg
    12841 ctgggagacg aggagacggg ggggacccgg tagagatcac atgcgcgaag attttgagta
    12901 tggtttataa ttcagaactc tactctgcga aactttgcat gtagcttggt gtactctgcg
    12961 gttaaaataa tgaacgtggg tttggagact taaaagttaa aaattaaaag gctgaggttg
    13021 tatatgttag tattaaaaat acttcacaaa atcttattcg tactttcttc ctatttccca
    13081 ttctctcaag tcccccttcg actccttttc catcaatcgc ccagcattat agtctcccat
    13141 ctaaccttct tctaataaca tgcacaatga ttccttcgac atttttttag aagcaagcgc
    13201 tggctattcg ataaatattg tcggatcggc ccatggtata gaaatgaaca gcaggaacat
    13261 gagctgctat caactctcgg gtttgatgaa ttgtccattc tattcctaaa ttccgcgctt
    13321 cctgactggt tttgcacttt tccaattctt ttgccaagtc gtcgggaagg tcgatgccaa
    13381 aggttcgggg caataaatgc agatgttgac aaatggctac gggtttcagc cctgggatga
    13441 tgggaacatt tatacctatc tcatggcaat tgtcgacaaa gtcgaaatat ttccgattgt
    13501 cgaagaacat ttgcgtaacg atataacttg ctccagcatc tattttcatc tttagatatc
    13561 taagatcgga tgccttattg ggggcttcgg gatgtttctc gggataacct gccacaccaa
    13621 tacaaaaatt ggttggatta gaattttgca aatcctcttc gagatatttt ccatgattta
    13681 aatcaacgat ctgttttacc aagtcgaggg aatgaatatg accatcaggc tcgggaatga
    13741 aggttttttg gctacggtcg ggatcccccc tcactacaag tacattatta ataccaagaa
    13801 aactaaggtc gatgagtgca ttttcggttt cttcacgtgt aaaaccacca caaataatat
    13861 gaggaactac ctgaatgtta caagtatatt gtaaggcagc agcaatacca actgtcccgg
    13921 gccgctttct tactaccatc ttttctacca agccattgcc acgatcttta tagaccactt
    13981 cttcctgatg ataagtaaca tttatgtaag caggtttaaa ttccaataaa ggttcaatac
    14041 tattaaaaat atcattgaat gaacctcctt taaggggagg tagaatttca aatgaaaaaa
    14101 gtgttttatt ggttttcgag agaatttcac aaacatgcat gggtaaaaat caagttaagg
    14161 gttcataatt caaatgggtt cgcagcaatt tttccacttc acgtatgcta agttgttttc
    14221 gcaaggcata gtcttgtatc tgatcgggat ttatttttcc cacattaaaa tattttgctt
    14281 gaagatgcga aaaataataa ccacaaaccg aagctgctgg gtacatagca taatgggaag
    14341 taagacttat acctatttgc ttttcggcat cgagcatttc gaaaagagtg acttttccgg
    14401 aatggtcagg acaggcagga tagcccggcg ctggtctaat tccctgatat ttttctttca
    14461 ggatatcttc cattgaaaga ttttcatccg gagcgtaagc ccagaactcc cttctcactc
    14521 ttttatggag taattcggca aaggcttcag ccaaacggtt ggccaaaatt ttaagcatta
    14581 taccactata atcgtcatga tttttatcga atctgtcaag ctgagtttct atgcctattc
    14641 cagtggttac cgcaaatgca ccaaggtaat cttggcgctg gctttctttg ggagctataa
    14701 aatcggacag gcataaattg ggaattccat cgggtttttt ttcttgattt cttaaaaaat
    14761 gcaatatttt ccaaacccta ccgtcgggat gataaacttc tatactatcg cccaaactgt
    14821 tagccggtaa aacaaaaaat gccgcattgg cctgaagcca tttattttga ataatcttat
    14881 agagcatttc ctgggcgtcg gcatataatt tattggcttc ttctcctttt acagggtcat
    14941 cgaaaaggga aggatatttt cctgtaagac gccatgcatg gaaaaagaat gtccagtcga
    15001 taaagggaga aatttcgctc agatcatatt catgaaaata ctttgtacct atgaatgcag
    15061 gttggcaaat aggctcttta tcccaatcca ttttcaggcg attacttctt gcttgattta
    15121 tagtgatgta tgtttgagaa acattctttg tggcttggta cttttcacga agattctcat
    15181 attttagttg aatgtcggcc caatattttt gttgctgtga atgatttatc aattgattag
    15241 cgacatttac ggcaagtgag gcatctttaa catgaatgac aggataggag cgttttgggg
    15301 ctatgcgcaa agccgtatgt aattcacttg tagtagcccc tccaatcagt aaaggttgag
    15361 tcattcctct gatttccatt tcttcggcca caaggcacat ttcttcgagt gaaggggtta
    15421 tcaagccgct gagccctatc atatccacct tattttcaat tgcagtctcg agaatagtct
    15481 tggcaggtac cattactcct aaatcgatga tctcataatt attgcacgat aagactactt
    15541 tcacgatatt tttgccgata tcatgaacat ctcccttcac tgtggccaaa agtatctttc
    15601 cagcagaagt ctgattattt ccattggttt ccttttcttt ctccagatag ggtgtaagaa
    15661 aatcgacggc ttttttcatc actcgggcag ctctaatcac ctgaggaaga aacatttttc
    15721 ccgaaccaaa cagctctcct ataaggttca tcccttccat caaaggtcct tcgatgattt
    15781 gaagcgcaga gggataagtt tcgagagcct ccttcatatc ttcttccaga aaatcactta
    15841 ttccttttat caaggcatgc tcgagacgtt tctccaatga atcgcttctc cagttatgag
    15901 ttattaagct atctatttcg gcttttttgc cgtattggtt cgcatacatc accagacggc
    15961 ttgtggcatc tttacggcgg tttaatatta catcttccac caaagtaagt aggggttcgg
    16021 ggatttcttc atacacgggc aaggcccctg cgttcacgat gcccatatcg agtccggctt
    16081 gtatggcatg ataaaggaaa acagaatgta tggcctggcg tatcttgtcg ttgccacgaa
    16141 aactaaaaga aagattacta attcctccac tggttcttac accatgcaaa ttttccttta
    16201 tccatttcac ggctctgata aattctacag catattgatt gtgttcgctc atgcccgtag
    16261 cgatagccaa tacattggca tcgagtataa tgtcgcttgt atggaaaccc actttatggg
    16321 tgagtaaacg aaaggctctc tggcaaatgg agattcttcg ttcgaaagta tcggcttgtc
    16381 cgttttcgtc gaaagccatc actacaaccg ctgcaccata atccagaata gtccgtgcat
    16441 gttgtataaa gactttttct ccttctttga gactgatgga attgacgata cattttccct
    16501 gagaacattg cagcccggtg tctattacac tccatttgga agaatctatc atcaaaggaa
    16561 tccttgcaat ttcgggatca gacatgagat ggtgaagaaa tatcttcatg caacgttcgg
    16621 aatcaaggag agcgtcgtcc atattgatat cgagaatctg agctccgttt tcgacctgtt
    16681 gacgagccac ctgaagggct tcttcgaatc tttcttctcg aataaggcgg gcaaatttct
    16741 tagaaccgct cacattggtt cgttctccaa tattaatgaa gttgcttccc ggaaattgaa
    16801 ccagagggtc aagtccactg agcataagag cttcatgacg aggagatgga agatgtggct
    16861 gggaaagatt taccatttcc acgagttttc gaatgtgctc gggggtcgta ccacaacatc
    16921 caccaataat attcactttg gaagcatgaa gaagaggtgc gagatcatgg gccatttcat
    16981 cgggcgactg ctcatattcg ccgaattggt tgggcaggcc ggcattggga taaacactta
    17041 tgtaaaaagg tgccttggag gcaatttctt ctatatatgg cctgagttgc agggctccca
    17101 tcgaacaatt gaaaccaatg ctcagcagct cggcatgtga aagagaaatg aggaatgctt
    17161 cgggtgtttg gccagagagg gtacggccgc tagcatcgct aattgttcct gaaaccatga
    17221 tgggaaatct gcgttttaac cccctggcag tcatcacaat gtctttccct tttgcctgaa
    17281 gaaccctttt gcaagccaac aaagccgctt tggcattcaa ggtgtcgaat atcgtttcga
    17341 ttaacagaat atctactcca ccatcgagca aagcctcaac ctgagtaaca taagcctctt
    17401 ccatttgctc gaaacttatg gctcggttag ctggattatt tatgtcggga gaaagtgaga
    17461 gagttttatt ggtggggcca atagatccgg caacgaatcg tggtttcgtt ggtgtggaat
    17521 attgtctggc tacctcaata gctaggcggg cagcctcata attcatctgg taaacaagat
    17581 gttcgagttt ataatcggct tgtgagatgg catttgcatt gaaggtgttg gtttcaatga
    17641 tatattgctt ccgtctagat tcgttacata ttcattaatt accaaatctg aagttcctgc
    17701 gccaatatca atggatgcaa ttttcatttt tttatttggc aataagatat cagcgataac
    17761 agatggattg tttttgattt tttttgtcaa taagctatac aaaaatacca attgacatga
    17821 agttg
//
LOCUS       DAOORU010000028        17541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_3941_length_17541_cov_5.523962, whole genome shotgun sequence.
ACCESSION   DAOORU010000028 DAOORU010000000
VERSION     DAOORU010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17541
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3941_length_17541_cov_5.523962"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLF20_03270"
     CDS             complement(<1..575)
                     /locus_tag="PLF20_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80062.1"
                     /translation="MYKSLLFTLAVWLILILPGFAQNEVVYPTSITKAVYFDVSLPLR
                     DIIPIPPEEADRTWKSGVVKNFLNLRQPDTTHIVDMVAQKFPGKWISSGIGVNINGIG
                     NINNVMPPDTDGDVGPNHYFQMINLSFQIFNKSGISIYGPAANSTIWSGFPGPWSGTN
                     DGDPIILYDEFADRWIASQFALPNYPNGPFWV"
     gene            complement(1181..2455)
                     /locus_tag="PLF20_03275"
     CDS             complement(1181..2455)
                     /locus_tag="PLF20_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HOX80063.1"
                     /translation="MKQKLLFFGLFIFIGLARVAAQDEFRVDAELRPRFEVRRGYLKL
                     AQPDDAASVLFSQRTRLKFSYLSKKLDIVVSPQDVRVWGDEENASIAGVSGNDASLDL
                     FEGYADLKFSSSVSLAVGRTVLAYDNEWLLGRSNWNQNGISSDALLLKINPQGWKFHL
                     GWSWNSLKETLKDNFYPPDRYKSMGFIWINRQSNQNFTFSFLQLATGQTPSDNTNKQY
                     FRNTSGFYLNANAGRLGGSLNLYYQYGRNNRNEKVSAMLCVAEASYKTLRWRPVAGLV
                     YSTGNRAGTSTDHLFDLAYSARHNILGNLDYFKNLITDTHQGGLTDVYAKIEYASSKS
                     LKFSNTSHYFWLAQTNELTPAQKKLGFENDFVAAYNLKEMGSLEAGYLFFLPTHGYRE
                     MQSVDGNAFSHFFYVQLTIFVKMFHSGNKKEG"
     gene            complement(2616..3497)
                     /locus_tag="PLF20_03280"
     CDS             complement(2616..3497)
                     /locus_tag="PLF20_03280"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HOX80064.1"
                     /translation="MNDSTSRDTSKQYNALSPEEERIIVHKGTERPFSGKYYDFWEEG
                     TYVCKRCNASLFRSAAKFDAGCGWPSFDEEIPGAVIRQPDTDENRTEILCAKCGAHLG
                     HVFTGEDFTPKNIRHCVNSLSLRFIPQKSSQTSDTAIFAGGCFWGVEYHFKNVKGIIS
                     TRAGYTGGTLSHPTYQQVCSGSTGHAEAVEVIFDPEQLSFEDLAKLFFEIHDPTQLNR
                     QGPDVGLQYRSAIFYFDDEQKAITEKLISILKSKGFDVLTELKPASTFWPAEDYHQDY
                     YEKTSKKPYCHIYTKRF"
     gene            3853..4221
                     /locus_tag="PLF20_03285"
     CDS             3853..4221
                     /locus_tag="PLF20_03285"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOX80065.1"
                     /translation="MHKANISKELRFAVGLVVIFTLSCEKDKPVNPYPNVFSPIASFD
                     YTMNVLENVVRVSFINTSQNADAYLWNFGDGTTSTLVNPVKDYPRPVAKPKSYNVVLT
                     AFDTVNDTYNRTSTTLLFEP"
     gene            4248..4727
                     /locus_tag="PLF20_03290"
     CDS             4248..4727
                     /locus_tag="PLF20_03290"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:TIGR00589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOX80066.1"
                     /translation="MIFKTYLESPIGLLEITGDKNGIHSVYFENKFEKPHNLSLTCQG
                     CPDPLLECRRQLREYFEGWRKTFNLPFILNGTDFQKKVWTQLSKIPYGSLISYSALAS
                     QLNEPMTVRAVAGAVAKNRINIILPCHRVVGAHGHLTGYGGELWRKKWLLGFENPHV"
     gene            5091..6557
                     /locus_tag="PLF20_03295"
     CDS             5091..6557
                     /locus_tag="PLF20_03295"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX80067.1"
                     /translation="MRKTLIGLLVFFSLSASAQEVLTLEVCKQIALSNNRILRIDSSN
                     LQSAKYSSKSAFANYFPDISFSGTYLRLNKQFQMFKNDLLLPVIPYQAIEAANMQVSI
                     SSLNNPQIAPLAFALDPATGMPFTDANGHPVFKNYAWLPASQSKLGEKDNFLFNLSLT
                     QPIFVGGKIYFANKMAQNAVEIAICKTEIDRQSLLYEVEDLYYKVWITEKKYRLALKY
                     LDLLKEIENDVENYLKEGLITSNQLLMIRSKINQAQVDIFKAENGLSVQKQLLNQKIG
                     RDIKQDFKVEDSLKADFEIIQNILNTQLSIDNRPEVKLLEQKVQLNRNLMRVTQADMM
                     PQLGLTANYFYINPNPYKGLEDEFGDDYALGLSLKIPLFHAFERYNKRQSALLQYKSA
                     QLEMEDSRNLLNIEATYVTNQLKEQLMQIEAARKNVELARENLRIQTDMFHEGMLKLS
                     DLTEAQTMWLKAETDYYEAYASLLSYHAKLRKSYGILQ"
     gene            6571..7629
                     /locus_tag="PLF20_03300"
     CDS             6571..7629
                     /locus_tag="PLF20_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX80068.1"
                     /translation="MKKSIVSLGFIGIILLGGCNDKNRQNPSNHGGEKPKVEIYRIHT
                     QDVPLNLSYLGSFKPFKEATLGTTIPGKIEKIYTQEGEAVKEGQLLVQMSSELLLQSE
                     IEYKTLEKDFQRVKNLVEKQSISQQDFDHVKARYEAAEAKYNLLRSNTEIRAPFDGIV
                     AKHLAHEGENYFFSASIDVGTSLSTGILQLVKINPIYFEFEVGEKDLSYISKNMVVKA
                     IANTYSHDTIAGWVDFISPTLNSTRATANIRIKINNPNLKFRPGMTGKAFINVISHQV
                     MMIPLQAMMKELNHDAYFVYAVDNEGKIIKKPIKVFQYAGENVVVEGVSEGDEVVISS
                     KKNLAEGIEVEIIPTSND"
     gene            7641..10739
                     /locus_tag="PLF20_03305"
     CDS             7641..10739
                     /locus_tag="PLF20_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOX80069.1"
                     /translation="MRITDTSVKNPVAIYMIFAGILLFGLLAIFRLPRDLFPDIELPT
                     LTVVTVYPGANTEQVEKEITKELEVVLASTENIKKIQSFSKDNVSTISLQFDWGTDIT
                     EASANARDLIELVKYKLPRDAQQPIIMKINSSVMPVVILGLQTTNEETDISDLIEKVI
                     SPRLQHIDGVGTVMTIAEPENEIAVEIDPLKAEKYHLTIDYIATLMKSLNLNVPAGSL
                     KSGIWDLNVTVKGQVPSIDAIKDLPLTTYMGQVIRLADIAIVHKQFKQKDEIARTHQQ
                     RATALLVQKQSQANALQVYQQVMKEVSKIPMPKETSLYEVFNTTEVIQGSINNITQTL
                     LWGGLWVILVVWLFLRAWRSSFIISLSIPFSMFIALITIIVSGYTINVFTLMSMVVAI
                     GMVVDNSIVVVENITKYLEKGIKKKEASIFATGEMGRAITASTLTTIAVFFPLIFAGG
                     IVGVFFEQFAIIGAATMLGSLLIALTLTPTLSSQFLLEESKQKKHPQIYDTTEKWYVK
                     LEEAYGRLIKWSLHNKILVTIIVAAFIALSVFLINNHIGTDYIPNIDGGDLITVVQMP
                     EGYSANETEKVAMRIEKYIESNVSGIVFGFTVAGQTEQGILSSVGFKENKNLASIVYH
                     LVPSNQRTMSTAEVAKMVEKELEKMPEIEQYHVTGGSIIGSALLGTTKPIEIVISGTD
                     LEILRKYAKMVEDSLMQLPDLKNVQNSLANTKPEFQITLDKERMADLKINPALASMQI
                     RQSLYGTEAGNFDDQGKQIPINVRYLRNEVQSINDIMNIRISSVLGNSYPLREVAEVN
                     LNNGYVEIMHDNQQRVAIVGADINSGTDLGVMVKRVQNVLSHLDTDNQVRIELKGQAE
                     EQAKSFADLRLILIISVLLVYMIMAAQFGHLLQPFIIFLSSPFAVVGSIIAFYLTGTT
                     LNLISFVAIILLMGVVVNNGIVLVDYTNMLVKRGYSLYDALIAAGRSRLRPVLMTTLT
                     TIMGMLPMAINRGLLHEVWAPFGITSIGGLMFATLITLIWIPVLYAIFYAHIQPKKLL
                     NV"
     gene            10736..11041
                     /locus_tag="PLF20_03310"
     CDS             10736..11041
                     /locus_tag="PLF20_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80070.1"
                     /translation="MKHVNIHCDVAQANEIIEILEECLISAFQILDNVKVKNRIGDPR
                     MNNPIWPGYNVLIMTQIREQAKFEQLMHKIKDYNIKVTHLDQLIMLEAWDIDTLIFE"
     gene            11041..11622
                     /locus_tag="PLF20_03315"
     CDS             11041..11622
                     /locus_tag="PLF20_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX80071.1"
                     /translation="METKKNKTRNLILKKAAEVFTAKGIQNTTIEDVTKHLGKAKSLI
                     YYYFSDKETLYKEVLENEINSLRNKVNEKLNSTVDPISKLRMYFELRIQKTKELINLL
                     PFKNHLDENLPFIKEMRKKYDDEETTIIKKILEEGAKRNIFYLTDLNNTTNAILTALK
                     IAELPYLISEYIDNYDEMMDSLIDIVLYGIVKR"
     gene            complement(11755..13710)
                     /locus_tag="PLF20_03320"
     CDS             complement(11755..13710)
                     /locus_tag="PLF20_03320"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOX80072.1"
                     /translation="MNNKKIHTVCSLIFTFLFAGLNIFEYSFVHTLEAQNLHLMARGK
                     IFPTQNSLDTLETLPPPLPGDKYDIVFMKLELELDPSIHYVKGKAKIYIIPKEDSDSI
                     SLQLHDSLQVEYVNFHGNFLNYSRPGIHALIIKFPEFLHKGELDSLEIMYQGDPPEYL
                     GENAFFTGFHNGKPILWTLSEPYGAFTWFPCKITLDDKIDSTDIVIQCHLDYQAASIG
                     MPVKTDTLEGWVKTSWKHRYPVASYLIGVAVGDYQTYIEEVPTQYGTITTYNYVYSGD
                     ISQQQAATSGLFPVYALYSELFGPYPFIEEKYGHLECGMGGGMEHQTLTFAGQFNHHI
                     LSHELAHSWFGNKITTGSWTDIWLNEGLASYATGLTYQYMFDGFYWKKWKTETIAAVC
                     QQPNGSVIVDDTTFAGRIFDARLSYHKASLVLHMIRFLIGDSAFFRALQNYAANPLLS
                     YGYARTFDLIGQFETSSGINLSEFTAQWLYGEGYPTYTIDWGISGNDTLELSIWQQTS
                     SPSVAFFDMPVEIGIKGAMNDTLIRLNPSFSGEQFQIPLTFTPDSLVFDPNLWLISAN
                     NRVNGIKYEPHETLRVYPNPATSFFYLPVTFSLPIQLTVFDLSGRIIRQCQVIQRSVP
                     VDDLEAGIYIIALSNGKTTAYTKLIKQ"
     gene            complement(13804..14472)
                     /locus_tag="PLF20_03325"
     CDS             complement(13804..14472)
                     /locus_tag="PLF20_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80073.1"
                     /translation="MDGISNEELFSTLNDKGNLKKEIYQNTERAFELINQAARKISQI
                     SPMKDEHLRIEFNRISPFQSQLFFAGDALIFFMQTDMYEFSRDHDVMRTAYIRQDKTR
                     SYVGIIHIYNFLTDSFKYNRENDLGYLIGRLFINKNMHYFIDGKREVGILYPQFGTLL
                     FDETAAENLIKSSMLYTINFDLLTPPFDEVKVITAGAVIHYAENCRLVIGKRLGFRFQ
                     ADND"
     gene            complement(14487..15263)
                     /locus_tag="PLF20_03330"
     CDS             complement(14487..15263)
                     /locus_tag="PLF20_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOX80074.1"
                     /translation="MMRQIRKIHFLGFLFLLISVMVYSQEGPSVMNLPKFDRASRHFG
                     FYVSGNQMFFTIQPADKFQQKVWNRDQIPDLVADSARVIGIESSPVPGFSIGLVSNLR
                     LGEYFDLRFLPGLQFGERELHYSVMQFSHADSSIVNISKNINSTFIDFPLYVKYKSKR
                     LNNFRAFVTAGASYKIDLASSTKDKAENPSNVVVKLKKHDFTGDLGVGFDFYTVYFKF
                     STEIKMSYGINDMIYREGNIYTDSIKKLSAKMFQISFTFE"
     gene            complement(15313..16008)
                     /gene="ubiE"
                     /locus_tag="PLF20_03335"
     CDS             complement(15313..16008)
                     /gene="ubiE"
                     /locus_tag="PLF20_03335"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOX80075.1"
                     /translation="MENHRSTQPILFDEIAPRYDFLNHLLSVGIDISWRRKLVNRLPE
                     NNQPYILDVATGTADLAIMAAKRIPCRIIGYDIADRMLEIGERKIAQNSLTHKIQLIR
                     GSVEDLPFADNSFDAVMVAFGVRNFKNLPKGLSEMYRVLKPDGISLILEFSIPENKIV
                     RNLYHFYFFRIFPLIGGWLSKNRKAYTYLPESVQQFPQGKHFERMLKGMGFRRTTFQS
                     LTFGIAILYEAIK"
     gene            complement(16026..17045)
                     /locus_tag="PLF20_03340"
     CDS             complement(16026..17045)
                     /locus_tag="PLF20_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOX80076.1"
                     /translation="MNDSQIETDLFDKFRNLRVLVVGDVMVDSYIWGRVERLSPEAPV
                     PIVDVLDRSNRLGGAANVAINIKSLGAEPILCSVIGKDSPSRTFVRLMKESGLDQDGI
                     LQSNNRITSTKYRIFGNNTQLLRIDEETNCPILPSDQSRLLQIIEFQVQTNHPDIIIF
                     QDYDKGVLTQGIIRDIMQLATEAKIKVCVDPKRINFWAYDDVLLFKPNLKELKEGLKM
                     NHIYQTDEFILKDAIIALQNKINAEIVLVTLSDEGMVIRYSVESEGFQYQSIPAHLRH
                     IADVSGAGDTVISVASLCLAAGFSPLLSAEIANVAGGLVCEEVGVVPINAKRLSEEIE
                     RLNLY"
BASE COUNT         5558 a         3434 c         3427 g         5122 t
ORIGIN      
        1 acccagaaag gaccattggg ataattagga agggcaaatt gcgaagcaat ccagcgatcg
       61 gcaaattcat cgtaaagaat gattggatct ccatcgttgg tgcccgacca tggacccgga
      121 aatccactcc aaatggtact attggcagct ggcccatata tcgatatacc actcttgtta
      181 aatatctgaa acgatagatt gatcatctgg aaataatgat ttgggccaac atcaccatcc
      241 gtatcgggtg gcattacgtt gttgatattg cctattccat tgatgttgac acctattcca
      301 ctgcttatcc atttccctgg aaatttctgt gcaaccatgt cgactatatg tgtggtatca
      361 ggctggcgca ggttgagaaa attctttact acaccactct tccatgtcct gtcggcttct
      421 tcaggtggaa tgggaataat atcacgtaag ggtaaactga catcaaaata tactgccttt
      481 gtgatagaag ttggataaac cacttcattt tgggcaaagc caggcaaaat aagaatcaac
      541 cagacggcta aggtaaaaag taatgatttg tacataattg atttataaat taattggcct
      601 tttctcttga attacttaat acaatcttga caatgaaatt gttggaattc aacaacaaaa
      661 atcgaggcac caaaactacc gattttcgaa aacattggct attatcctga aagttcattc
      721 caattcattc tttgttttga tcctgcgaat gtgtccattt aatatcattt attcctttct
      781 ttttgataaa gaattcattg ccttttactt gaaattcgat ttaaaatccg aaattttctg
      841 ataaccaaat taatcaaaaa agaaaagagt gggtaaaggt ataaaatatt cgtttgcacc
      901 acaattgttt tataacagta tgctaaaatt ttttatcaca taacttacct tcttatttat
      961 ttgcttgttc ctctatctat tagcctctta taaattagga tgaaagaaat acacctcaac
     1021 aaggtgatca aagcgatcat tgagaacagg taacgctata acgccatctt caaaatcatg
     1081 acaacctaat taatgctgaa tcaagagttc agaatttatg tggctatcat caacgacgac
     1141 aatgccatca ttactactaa tttttacata taaattgcat tcatccttct ttcttatttc
     1201 ccgaatggaa catttttaca aaaatagtga gttgaacata aaagaaatga gagaatgcat
     1261 ttccatccac agattgcatc tctctatagc catgagtagg aaggaagaac agatatccag
     1321 cctcgaggct gcccatctct tttaaattgt aagcagccac aaaatcattt tcgaaaccca
     1381 attttttttg tgcaggagtc agttcattag tctgagcaag ccagaagtag tggctggtgt
     1441 tggagaattt caacgatttg gaggacgcat attcaatttt tgcgtaaaca tcggtcaatc
     1501 ctccctggtg agtatctgta attaggtttt taaaatagtc caaatttcct aatatattat
     1561 gccgtgcgct ataggcaagg tcgaataaat ggtcggtaga agtgccagcc cgattgccgg
     1621 tactataaac caaaccagct actggccgcc atcgaagggt tttgtaactg gcttcggcaa
     1681 cgcatagcat agccgaaact ttttcatttc tattgtttct gccatattga tagtagagat
     1741 tgagagaacc acccaagcgt ccagcgttgg cattgagata gaagcccgaa gtgttcctga
     1801 aatattgctt attggtatta tcggaaggtg tttgccctgt ggcaagttgt aaaaaggaaa
     1861 atgtaaaatt ttgattgctt tggcgattga tccatataaa acccatggat ttataccgat
     1921 cggggggata gaaattatct ttcagggttt ctttcaggga attccagctc cagcccaaat
     1981 gaaatttcca tccctggggg tttattttca ataagagagc atcgctcgaa atgccattct
     2041 gattccaatt gcttcgaccc aataaccatt cgttatcgta agccagcacc gttcgaccaa
     2101 ccgccaatga aacagaagaa gaaaacttca aatcagcata tccttcgaac aaatccaacg
     2161 aggcatcatt gccactgact ccggcgatac ttgcattttc ttcatcaccc cataccctca
     2221 catcctgagg tgaaaccaca atgtcgagct ttttgcttaa ataactgaat ttcaggcgtg
     2281 tacgttgaga aaatagaaca gaagcagcat catcaggctg ggctaatttc agataacccc
     2341 tccttacctc gaatcttggc cttaattcgg catcgaccct aaattcatct tgggctgcaa
     2401 ctctcgctaa tccaataaaa ataaataatc cgaaaaacaa gagtttttgc ttcatacaga
     2461 tcgagtatta atgaatattg tcctcattta tagacaagat atctcacctt tccttactat
     2521 atcgggcaag aaattgtgat ttatagtcaa aatgagataa tagaatcaat agggctcaaa
     2581 aatacgggaa aattgtcagg ttattcccat ctacttcaaa atcgtttggt ataaatatga
     2641 caataaggct ttttgctggt tttttcgtaa taatcctgat gataatcttc ggctggccag
     2701 aaggttgaag caggtttcag ctctgtaagc acgtcaaatc ctttcgattt cagaatagag
     2761 ataagttttt cggtaatggc tttttgttcg tcatcgaaat aaaaaatagc cgagcggtat
     2821 tgaagtccca catctggtcc ctgtcggttc agttgcgtgg gatcatggat ttcgaaaaat
     2881 aatttggcta agtcttcaaa agaaagctgt tcaggatcga agattacttc aacagcctcg
     2941 gcatggccag tacttcccga acatacctgc tggtaggttg gatggctgag tgtacctcca
     3001 gtgtatccag ctctagttga aattattcct tttacatttt tgaaatgata ttctacgccc
     3061 caaaagcagc caccagcaaa aatggctgtg tcagaggttt gagacgattt ttgaggaata
     3121 aatcgaagcg aaagactatt gacacaatga cggatatttt taggcgtaaa atcctcaccc
     3181 gtaaatacat gcccgagatg cgctccacac ttcgcacata aaatttcggt acgattttca
     3241 tcggtatcgg gttgtcgaat gacggctccg ggaatttctt catcaaagct tggccagcca
     3301 caaccagcat cgaattttgc tgccgaacga aacaaagaag cattgcatct tttgcataca
     3361 taagtccctt cttcccaaaa atcgtaatat ttcccactga agggtctttc ggtgcctttg
     3421 tgcactataa ttctttcttc ctcgggagaa agggcgttat actgttttga agtatcccta
     3481 ctggtagagt cattcatatt ggtatggcta gagttacaag atagtgagag taagaacata
     3541 gacaaaatta ctggaaatga attcatttcg aggttttcta aaagaacaaa aaagttggca
     3601 aaatgtttat gacttttcaa aatgaatgga tataatatga ggcacatgat ctttttaaaa
     3661 gatttcatca taagattgat tgggataatc gatagaaatt tggaaatttt tcgacatcgg
     3721 ctgttcatgt ttgctctttg gttatatttg caaactcctt ccttttttta tgatgccaat
     3781 gaacattatg catcgctcag aatttgtgag gattgaagat gaaaatagtt tagaaaatta
     3841 aaatttcgtg atatgcataa ggcgaacatt tcaaaggaat tacgatttgc agtgggtttg
     3901 gtggttatct ttactctatc gtgtgaaaag gataaacccg tgaaccctta tccaaatgta
     3961 ttcagtccca ttgccagttt cgactatact atgaatgtat tggaaaatgt tgtaagggtt
     4021 agttttatca acacctcgca aaatgccgat gcctatttat ggaattttgg tgatggaacc
     4081 acaagcacat tggtaaaccc ggtgaaggat tatccaaggc cagttgcaaa accaaagagt
     4141 tataatgtag tgcttacagc tttcgatacg gtgaatgaca cctacaaccg aacatctacc
     4201 accttgctat tcgaaccctg aagtattctt ttgttagcta atcttttatg attttcaaga
     4261 cttatctcga atcgcccata ggacttttag aaatcacggg cgataagaat ggaatccatt
     4321 cggtgtattt cgaaaataaa tttgagaaac cccacaattt gagcttaacc tgccagggtt
     4381 gtcctgatcc tttattggaa tgtcgccgtc aattacgcga atatttcgaa ggctggcgca
     4441 aaaccttcaa tcttcccttt attctgaatg gaacggattt tcaaaaaaaa gtttggaccc
     4501 aactttcgaa aattccgtat ggcagcctca tcagctattc cgccttggcc agccagctga
     4561 atgagcccat gactgtgcgt gcagtggctg gagccgtggc aaagaataga atcaacatca
     4621 ttctgccttg ccacagggta gtgggagcac atggccacct tacgggttac ggaggcgaat
     4681 tgtggcgtaa aaaatggttg ctcgggttcg aaaaccctca tgtttagctc gaattataat
     4741 gaatgggtta aaataagtaa tacaatagct tgggtttcga tcgctatcta tcaaatcttc
     4801 agttcttttt catgcgctta ttccaataaa tttggtagat caccatatct tcttttaact
     4861 ctcctgcggt ttctgacgaa atttttttat ccttgaatcg attccacttt ggataataac
     4921 agcattttaa cgcagggagt agaatagata ttgaactttc ttcaatattc atataaaaat
     4981 catttaaata taattatcta actatcaatg atttcaattt taaaataaaa attttttagg
     5041 gtgaacatca ttatattaga attttctact aattttgttc aaaaaaaatt atgcgtaaaa
     5101 cactcatcgg tttgctggta ttcttctcac tatcagcatc agcgcaggaa gttcttacgc
     5161 tcgaagtatg caagcaaatt gccctgtcga acaaccgtat actaagaata gacagcagca
     5221 atcttcaatc ggctaaatac agcagtaagt cggcctttgc aaattatttc cccgacatta
     5281 gtttctcggg aacatatttg agattgaaca aacagtttca aatgttcaaa aatgatctcc
     5341 ttcttcccgt aattccttac caagccatag aggcagctaa tatgcaggtt tcgatatcca
     5401 gtctgaataa tcctcaaata gcacctcttg cttttgcctt ggatcctgcc acgggcatgc
     5461 catttactga tgcaaatggc catcctgttt tcaaaaatta tgcatggctt ccagccagcc
     5521 aatcaaaatt gggcgagaaa gataattttt tgttcaacct ctcattgact cagcccattt
     5581 ttgtgggagg taagatttat tttgcgaata aaatggctca aaatgccgtt gaaatagcca
     5641 tatgcaaaac cgaaatcgac cggcaatcct tattgtacga agtagaagat ttgtactata
     5701 aggtttggat taccgaaaaa aaatacagat tggcacttaa gtatctcgat ttactcaaag
     5761 aaattgaaaa cgatgtggaa aattatttaa aggaaggctt gattactagc aatcagctac
     5821 tcatgatacg atcgaagatt aatcaagccc aggtagatat atttaaggca gaaaacgggt
     5881 tatcggtcca aaagcaactg ttgaatcaaa aaataggcag agatatcaaa caggatttta
     5941 aggtcgaaga ttctttgaag gcagattttg aaataattca aaatattttg aatactcaac
     6001 tcagtattga caaccggcct gaggtaaaac tgctcgaaca aaaagtacaa ttaaacagaa
     6061 atctcatgag ggttacacag gctgatatga tgcctcaact tggtttgact gccaattatt
     6121 tctatatcaa tcccaatccc tataaaggac tcgaggatga atttggagat gattatgcct
     6181 tggggttatc gctcaaaatc cctttatttc atgcttttga acgttacaat aagcgacagt
     6241 cggcattgtt acaatataaa tctgctcagt tggaaatgga agacagccgc aatttattga
     6301 acattgaggc cacttatgtt accaatcagc tcaaggaaca attgatgcag atagaagctg
     6361 ccagaaagaa tgtagaactg gcaagagaaa atttgagaat tcaaaccgat atgtttcacg
     6421 aaggcatgct aaaattatca gatttaactg aagcacagac aatgtggtta aaggcagaaa
     6481 ccgattatta tgaggcttat gccagtttac tctcctatca tgccaaatta cgtaaatctt
     6541 atggaatact tcaataaaaa cagaagaaat atgaaaaaaa gcatagtatc tctcggattt
     6601 ataggaataa tcctcttggg aggctgtaac gataaaaatc gacaaaatcc ttcaaatcat
     6661 gggggtgaaa agccaaaggt agaaatttac aggattcata cccaagatgt tccattgaat
     6721 ctttcttact tggggagttt taaacctttt aaggaggcta ctttgggtac tactatccct
     6781 ggaaaaatcg aaaagatcta tactcaagaa ggtgaagccg taaaagaggg gcaattgctg
     6841 gtgcaaatgt cgtctgaact cctgcttcag tcggaaatag aatataaaac tctcgagaaa
     6901 gattttcaga gagtaaaaaa cctcgttgag aagcaatcca tcagtcagca ggattttgat
     6961 catgtaaagg ctcgttacga agctgccgaa gcaaagtata atttattgcg gtcgaacacc
     7021 gaaattagag ctcctttcga tggcatagtg gcaaagcact tagcccacga aggcgaaaat
     7081 tacttctttt cggcatctat tgatgtgggt acttccttat cgacaggaat attgcaattg
     7141 gtaaaaatca atcccattta cttcgaattc gaggtgggcg aaaaagatct ctcctatatc
     7201 agtaagaaca tggttgtaaa ggccatagct aatacttatt ctcatgatac cattgcgggc
     7261 tgggtcgatt tcatcagtcc tactttgaat tcaacccggg ccacggctaa tattcgaata
     7321 aaaataaata atccaaacct caaatttcgc cctggaatga caggaaaagc ttttatcaat
     7381 gtaatcagcc atcaggtgat gatgataccc ttgcaggcca tgatgaaaga acttaaccat
     7441 gacgcctatt ttgtatatgc agtcgataac gaagggaaaa tcatcaagaa acccatcaaa
     7501 gtttttcaat acgcaggtga aaatgttgtg gtggaaggag taagcgaggg cgatgaagtt
     7561 gtaatttcca gcaagaaaaa tttggctgag ggcattgaag tagaaatcat tcccacatcc
     7621 aatgattaaa atctaaggct atgagaatca ccgacacttc agtcaagaat ccggttgcaa
     7681 tctatatgat ttttgcggga atccttctat ttggattatt ggctattttt cgtttaccaa
     7741 gagatttgtt ccccgacatt gagcttccta cgcttacggt tgtaaccgtt tatccgggtg
     7801 ccaataccga acaggtagaa aaagagataa ccaaggagtt agaagtagtt ttggcgagta
     7861 ccgaaaacat caaaaaaatt cagagttttt cgaaagataa tgtttccacc atttccttac
     7921 agttcgactg gggaactgat attacagaag cttcggccaa tgcacgcgac ttgatagaat
     7981 tggtgaagta taaactgcct cgtgatgctc aacaacctat catcatgaag atcaattcct
     8041 cggtaatgcc agtggtgata ctaggtcttc agaccaccaa tgaggagaca gatatcagcg
     8101 acctgatcga aaaggtaatt tctccgaggc ttcagcatat agatggagta ggcacagtga
     8161 tgaccattgc tgaacctgaa aatgaaatag ccgtagaaat cgatccttta aaagccgaga
     8221 aatatcattt aaccatcgat tatattgcca ccttaatgaa aagtctcaat ttgaatgttc
     8281 cggcgggttc tttgaaatcg ggtatttggg acttaaatgt tactgtaaag ggtcaggtgc
     8341 cttccatcga tgcgataaag gatttgccgc ttaccactta catggggcaa gtaatccgat
     8401 tggctgatat tgcgatcgta cataaacagt tcaaacagaa agatgaaata gcccgaactc
     8461 atcagcaaag ggcaaccgct cttcttgtcc aaaaacaatc tcaggctaat gcgcttcagg
     8521 tttatcagca ggtaatgaag gaagtaagca aaatccctat gcccaaagag actagtctgt
     8581 atgaggtttt taatactacg gaagttattc aaggatcaat aaataatatc actcaaacat
     8641 tgctttgggg aggattatgg gtaattctgg tagtatggct cttcttacga gcctggcgtt
     8701 cgagcttcat catcagcctc tcaatacctt tttctatgtt tatcgctctc attaccatta
     8761 ttgtgagcgg ctacacgatt aatgtgttta ccttgatgtc gatggtggtg gctattggga
     8821 tggtggtcga taatagtata gtagtggtcg aaaacattac taaataccta gaaaaaggca
     8881 tcaaaaaaaa ggaggcttca atatttgcta cgggcgaaat gggtcgagct attacagctt
     8941 ctacactaac taccatagcg gtgtttttcc ctttaatttt cgcaggcggt attgtgggtg
     9001 tattcttcga acaattcgcc attataggag ctgctacaat gcttggatca ttgctcatcg
     9061 ctctgacatt gacacctaca ctgagcagcc aattcctatt ggaagaatcc aaacaaaaaa
     9121 aacatccaca aatttatgat actacggaaa aatggtatgt taaactcgag gaagcttatg
     9181 gaagattgat aaaatggagc cttcataaca aaattcttgt aactatcatt gttgcggctt
     9241 ttatcgccct ttcggtattt ttaatcaata atcacatagg caccgattat attcccaaca
     9301 tcgatggagg tgatctcata actgtagttc agatgcccga aggttatagc gctaatgaaa
     9361 ccgaaaaagt cgcaatgagg atcgaaaaat atatcgaatc gaatgtttcg ggaattgtgt
     9421 ttggctttac tgtagcagga cagaccgaac agggtattct ctcatccgtt ggattcaagg
     9481 aaaataaaaa tcttgcctcc atagtctatc acctcgttcc ttccaatcaa cgcacgatga
     9541 gcaccgccga agtagccaaa atggtcgaaa aagaattaga aaaaatgccc gaaatagagc
     9601 aataccatgt aaccggaggt tctatcatag gttcggcatt acttgggaca accaaaccca
     9661 tcgagattgt catttcagga actgaccttg aaattttacg caaatatgca aaaatggtgg
     9721 aggattcact aatgcaatta cctgacctca aaaatgttca gaacagtttg gccaatacca
     9781 agccagaatt tcaaataact ctggataagg aacgcatggc cgacttgaag atcaatcccg
     9841 ctttagcctc catgcagatt cgtcaaagcc tttatggcac cgaagcagga aatttcgacg
     9901 atcaaggcaa acaaattcca atcaatgtca gatatttacg caatgaggtt caatctatca
     9961 acgatattat gaatatcaga atttcttcgg ttttaggcaa ttcttatcct ttacgcgaag
    10021 tagccgaggt gaacctcaat aatggatatg tcgaaatcat gcacgacaat caacaacgcg
    10081 tagccatcgt tggagccgat ataaattcgg gcaccgacct tggagttatg gtcaaacgag
    10141 ttcaaaatgt tctatctcat ttagatactg ataatcaggt aagaattgaa ctcaaaggac
    10201 aagccgaaga acaggcaaag agtttcgccg atcttagatt aatattgata atcagcgtat
    10261 tattggttta catgatcatg gctgcgcaat ttggccactt gcttcaacct ttcatcatat
    10321 tcctgtcttc tccctttgcc gtggtgggct cgatcattgc attctacctc acaggcacaa
    10381 ccctgaatct gatatcgttc gtagctatta ttttgctgat gggagttgtg gtgaacaatg
    10441 gcattgtatt ggtcgattat actaacatgc tagtcaagcg tggttattcc ctctacgacg
    10501 ctttgattgc agccggaaga tcacgactgc gacccgtatt gatgactacc cttactacaa
    10561 ttatgggaat gctgcccatg gccataaata gaggcctatt gcacgaagtt tgggctccat
    10621 tcggcataac gagcattggc ggcctgatgt ttgctacact tattacactt atctggattc
    10681 ctgtattgta tgctatattt tatgcgcaca ttcaacccaa aaaactcttg aacgtatgaa
    10741 acatgtgaat attcattgcg acgtggccca agcaaatgaa atcatcgaaa tactcgaaga
    10801 atgccttatt agcgcatttc aaattttaga caatgttaaa gtaaaaaatc gcataggaga
    10861 tcctcgtatg aataacccta tatggcctgg atacaatgta ttgattatga ctcaaataag
    10921 agaacaggca aaattcgaac aattaatgca taaaatcaaa gactataaca ttaaagttac
    10981 tcacttggat caactcatta tgttagaagc atgggatata gatactttga tttttgaata
    11041 atggaaacaa agaaaaacaa gacacgcaat ctgattttga aaaaagccgc tgaagtgttt
    11101 acggctaaag gcattcaaaa cactacgatt gaagatgtta ccaaacacct tgggaaagcc
    11161 aaaagcctga tttattatta tttctccgat aaggaaactc tttataaaga agttctggaa
    11221 aacgaaatca attctctgcg caataaagtg aacgaaaaac tcaattcaac cgtagatccc
    11281 atttctaaac tgcgaatgta ctttgaatta cgcatacaga aaacaaagga attaattaac
    11341 ctcttgcctt tcaaaaacca tctcgatgaa aacttacctt ttattaagga aatgcgaaaa
    11401 aaatacgacg atgaagaaac cactattatc aaaaaaatac tcgaagaagg tgctaaacgc
    11461 aatattttct atcttactga cctaaacaat accaccaatg ctattctcac agctctcaaa
    11521 atcgcagaat tgccctactt gatctctgaa tatatcgata attacgatga aatgatggat
    11581 agcctgatcg atatcgtact ttatggaatt gtgaaacgtt gaagaattgc acaattcttc
    11641 gtctcatttc aaatgcactt ctgcatataa tcctcgctta acaataagat ttaataacaa
    11701 agagctcatt gtaggaataa tcgaatccga aaaaatcaag gcatattgtt cagatcattg
    11761 ctttatcaat tttgtataag ctgtggtttt gccatttgat agtgctatga tataaattcc
    11821 cgcttcgagg tcatctacgg gtacagaccg ttgtatcact tggcattgtc ggatgattct
    11881 tccagatagg tcaaaaacgg ttagttggat tggcaatgag aatgtaactg gaagatagaa
    11941 aaaagatgta gccggattgg gataaactct cagggtttca tgcggttcat actttattcc
    12001 attcacccgg ttattggccg aaatgagcca tagattcgga tcgaaaacga ggctatcggg
    12061 tgtgaatgtg agtggaatct gaaattgttc gccggaaaaa gaaggattca agcgaataag
    12121 tgtgtcattc attgcgcctt ttattccaat ttccacgggc atgtcgaaaa aagcgaccga
    12181 gggagaagaa gtttgttgcc agatggaaag ctcgagtgta tcgttacccg aaattcccca
    12241 atctatggta tatgtaggat atccttcacc atagagccat tgggcagtaa attcggaaag
    12301 attgataccc gaactggttt cgaattgtcc aattagatca aaagttcggg cataaccata
    12361 agaaagcaag gggttagctg cataattttg caatgcccga aaaaaagcgc tatcgccaat
    12421 taaaaatcga atcatgtgaa gcaccaatga ggctttgtga tacgacaggc gcgcatcgaa
    12481 aatccggcca gcgaaggtag tatcatctac gatgacagaa ccgttaggtt gctggcaaac
    12541 ggcagcaatc gtctcggttt tccatttctt ccagtaaaat ccatcgaaca tgtattgata
    12601 agtcaagccc gtggcatacg aagccaagcc ctcatttaac caaatatcag tccaggaacc
    12661 cgttgttatc ttgttcccga accatgaatg tgccagttcg tgcgataaaa tatgatgatt
    12721 gaattgaccg gcaaacgtca aagtttgatg ttccatacct ccacccatgc cacattccag
    12781 atgtccatac ttttcttcga tgaaaggata aggaccaaat aattctgaat aaagcgcata
    12841 caccgggaaa agccctgacg tagcagcttg ttgctggctt atatctccgg aataaacgta
    12901 gttgtaagtt gttatggttc catattgagt agggacttcc tctatataag tttgataatc
    12961 acccacggca acacctataa gataagaggc aactgggtaa cgatgtttcc atgacgtttt
    13021 tacccatcct tcgagggtat cggttttgac gggcattcca atgcttgctg cctgataatc
    13081 taaatggcat tgaatcacaa tatccgtaga atcgatttta tcatcgaggg taattttgca
    13141 tgggaaccaa gtaaatgctc catagggttc ggatagtgtc cacaaaattg gcttgccatt
    13201 atgaaaacct gtaaaaaacg cattttctcc gagatattca ggtggatcac cctgatacat
    13261 gatttccaag ctatctaatt ctcctttatg cagaaattct ggaaatttga tgataagcgc
    13321 atgtatccca ggtctcgaat aattcaaaaa atttccatga aaattaacat attccacttg
    13381 aagcgaatcg tgtaattgca aagaaatgct atcgctatct tcttttggaa taatgtaaat
    13441 tttggctttc ccttttacgt aatgaatcga ggggtctaat tcgagttcca acttcataaa
    13501 aacaatgtcg tatttatcgc cgggcaatgg tggcggcaat gtttcaagtg tatcgagcga
    13561 gttttgagta ggaaatatct ttcccctggc cataagatga aggttttgag cttcaagagt
    13621 atgaacaaaa gaatattcga aaatattcag gccagcaaag aggaatgtaa aaatcaatga
    13681 gcagacggta tgaatttttt tattgttcat ggcttacaaa tagaagataa aatcttcctt
    13741 aaaaataaat catgggacga gaaaacgaaa aattgaataa aaaatcaaag agatcggaaa
    13801 acattagtca ttatcggcct ggaatctaaa ccccaaacgt ttgccgatga cgaggcggca
    13861 gttttcggca taatgaatga cagctccggc tgtaatcact tttacttcgt cgaagggtgg
    13921 agtcaacaga tcgaaattaa tggtataaag catgctcgat ttgattaggt tttcagccgc
    13981 agtttcatca aacaaaaggg ttccgaattg tggataaaga ataccgactt ctcttttacc
    14041 atcaataaaa taatgcatgt tcttattgat aaaaagccgt ccgataaggt agcccagatc
    14101 gttctcacgg ttgtatttga aagaatcagt caggaagtta tagatatgaa taatgcccac
    14161 gtagctccgg gttttgtctt ggcggatgta agccgttcgc attacatcat gatcgcgcga
    14221 aaactcgtac atgtcggttt gcataaaaaa tatcaaggca tcaccagcga aaaataattg
    14281 gctttggaat ggacttatcc ggttaaattc aattctcaaa tgctcatcct tcataggtga
    14341 aatctgagaa atcttgcgtg cagcttgatt gatcaattca aaagcccgtt cggtattctg
    14401 atagatttct tttttaagat tgcccttatc gtttaatgtc gaaaaaagtt cttcgttcga
    14461 aattccgtcc atagtgtgta tattagttat tcaaaggtaa aagaaatttg gaacattttt
    14521 gcagataatt tcttaatgct gtccgtatag atgttccctt ctctatatat catatcgtta
    14581 atcccatacg acattttgat ctcggtcgaa aatttaaaat aaacggtata gaaatcgaaa
    14641 cctactccta aatcacccgt aaaatcatgt tttttcaatt ttaccacgac attggaggga
    14701 ttttcggcct tgtcttttgt tgaagaagca aggtcgattt tataagacgc tccggcagtc
    14761 acaaaggctc ggaaattatt taaacgcttc gatttatatt tgacataaag cggaaagtcg
    14821 atgaaagtag aattgatgtt tttcgaaata ttgacgatag acgaatcggc atgtgaaaat
    14881 tgcataaccg aataatgcag ctctctttct ccgaactgaa gtcccggaag gaaccgaagg
    14941 tcgaaatatt cacctaagcg taagtttgaa accaaaccaa tggaaaagcc cggcacagga
    15001 gaactttcga taccaataac ccgggccgag tcggccacaa ggtcgggaat ttggtcgcga
    15061 ttccaaactt tttgttgaaa cttatcggcc ggttgaatcg taaaaaacat ctgattacca
    15121 gatacataga atccaaaatg tcgagatgcg cgatcgaact ttggtaaatt catcaccgat
    15181 ggaccttcct gagaataaac cattaccgag atcagaagga ataaaaaacc gagaaaatgt
    15241 attttgcgta tctggcgcat cattcgattt gatggataaa cgaaagcgat agagctttat
    15301 ttttgatttg ttttatttta tagcttcata aagtatggct atgccgaagg taagcgattg
    15361 aaatgtggtg cgacgaaatc ccattccttt caacatcctc tcaaaatgct taccttgagg
    15421 aaattgttgt accgattcgg gcaaataagt gtatgccttg cggtttttcg acaaccaacc
    15481 acctattaag ggaaatattc taaaaaaata aaaatgatac agattcctta cgattttatt
    15541 ttctggaata gaaaattcaa ggataagtga aattccgtcg ggttttaata ctcggtacat
    15601 ttcagaaaga cctttcggca gatttttgaa gtttcttacg ccaaaagcca ccattacagc
    15661 atcgaatgaa ttatcggcaa agggaaggtc ttcaacagaa cctcgaatga gttgtatctt
    15721 atgcgtgagg gaattttgtg caatttttct ttcccctatt tcaagcattc tatcagcaat
    15781 atcgtaaccg atgattcggc agggtattct ttttgcagcc ataatagcca gatcggccgt
    15841 gccggtcgct acatcgagta tataaggttg gttgttttca ggtaaacggt tgaccagttt
    15901 tctacgccat gaaatatcta ttcctaccga aagaagatga ttgagaaagt catatctggg
    15961 tgcaatttca tcaaaaagaa taggttgagt actcctatga ttttccatgc ttcgttatcc
    16021 agttttcaat atagattcaa tcgttcgatt tcttccgaca agcgttttgc attaattgga
    16081 actacaccca cctcttcgca aaccagaccc ccggcaacat tggcaatttc ggccgaaaga
    16141 agcggtgaaa atccagctgc caaacaaaga cttgccacac taattacagt atcgcctgca
    16201 cccgaaacgt cggcaatgtg tctcaaatgt gccggaatac tctgatattg aaatccctca
    16261 ctttcaaccg aatatcttat caccatcccc tcatcggaaa gcgtaaccag aacgatttcg
    16321 gcatttatct tgttttgtag ggctatgatg gcatctttca gaatgaattc atcggtctga
    16381 tagatatgat tcatcttcag gccttctttc aattccttca gattgggttt gaaaagcaat
    16441 acatcatcgt aagcccagaa atttattcgt ttgggatcga cacaaacttt gattttggct
    16501 tcggtagcaa gttgcattat atcccgaatt atcccctgag tgagtacgcc cttatcgtaa
    16561 tcctgaaaaa ttataatatc gggatgattg gtctgaacct ggaattcaat aatttgaagt
    16621 aaacgagatt ggtcgctcgg taaaataggg cagttggttt cttcatctat tcttaaaagc
    16681 tgtgtattat ttccaaatat tcgatatttg gtcgatgtaa tcctattgtt gctctgcaaa
    16741 ataccgtctt ggtcgagccc ggattctttc atcagtcgaa caaaagtcct cgatggggaa
    16801 tccttaccaa tcaccgaaca taatataggt tcggctccca acgatttaat attgatggct
    16861 acattagccg ctcctcctaa cctgttactc ctatccaaaa catctactat gggaaccgga
    16921 gcctctggcg ataaacgctc tacccttccc catatatagg aatctaccat cacatcacca
    16981 actacaagta ccctgagatt tcgaaattta tcaaagagat ctgtttctat ctgtgaatca
    17041 ttcatcttta agcattataa ttacaatgca aagataaact ttgcctttca gttttaccat
    17101 gataaacatg aacgcctcca tcaaggtttt ttgagcatga taaatagaaa gttattttcc
    17161 tcagttttcg ataaagaatt ttcccttgcc cgtattcgat ttaaaacatt aatgattatt
    17221 cacctgaatt cattccgaat ttgttattaa ggaagaaatt ccgatggcga tccttagaga
    17281 aaattcaata taatgatttg gactacatta taatataaca agagaaaagc atttcttctg
    17341 gccctgaatt tagagaaaga gaaaagcaat gagatggaga aagtgaaaga actcaaaaac
    17401 agcgaaattt tttacggata atttagacct atggataatg aatcgagagc cttgaaaaat
    17461 cgagtaggta gggagattgc tcccctgccc tctcacccca cccagcatgc tctcgcaccg
    17521 gacggtttca tctaagagtg t
//
LOCUS       DAOORU010000029        17236 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4036_length_17236_cov_6.272219, whole genome shotgun sequence.
ACCESSION   DAOORU010000029 DAOORU010000000
VERSION     DAOORU010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17236)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17236)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17236
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4036_length_17236_cov_6.272219"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(19..162)
                     /locus_tag="PLF20_03345"
     CDS             complement(19..162)
                     /locus_tag="PLF20_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80077.1"
                     /translation="MLTKGRENRGMEEGRIEIGGWRLGIGDWRLGVGDWRLLVVRCQF
                     KNT"
     gene            complement(433..906)
                     /locus_tag="PLF20_03350"
     CDS             complement(433..906)
                     /locus_tag="PLF20_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HOX80078.1"
                     /translation="MDWIWITIGAILLVLGFIGSFAPFLAGPPLAYLALPALQLTSFS
                     PFSQKTLILWGIATILITLLDYVIPIIGTRKFGGTKYGTRGSAIGLVIGFLFGPLGII
                     IGPFLGALIGELLGGKNLNYGLRSGLGSFFGFITSTLMKMIFVIALAIVFIRAIL"
     gene            966..2195
                     /locus_tag="PLF20_03355"
     CDS             966..2195
                     /locus_tag="PLF20_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX80079.1"
                     /translation="MGAELFIARRLIKPKKESLSGPSVRIAIASVSISLTVMISSVCI
                     LRGFQQEIREKVSGFAAHIQVHSFVSGVSQSENPPMTYDTGLIMKLRNYPGIRHVQAV
                     AYKTGMIQANQQILGITLKGGGPDLDMQLIKDKLVKGRLPLMGDTALRYEILISQNMA
                     DKLLLDTGQDIRMYFLNEGERSPRARKMKIVGIYNTGLEDFDQSFAFTDIRHIQRLNG
                     WTHKEIGMIEIILRNIDELDMISRKVYEDLPSHLNVNNIRQIYPQIFDWLELQDVNVL
                     VILILMIIVSAISMISALFIMIVEKTQLIGILKALGATNLSIRKIFIQTSSFIALKGM
                     VYGNVIGIGLMWLQKHLHLVKLPVESYFMEYVPVSLSLTNILIINITTLILCISSLLI
                     PTYTVTRVSPLRAIRYH"
     gene            2291..2851
                     /locus_tag="PLF20_03360"
     CDS             2291..2851
                     /locus_tag="PLF20_03360"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80080.1"
                     /translation="MSEIEMKGKKKHGKAIYYYPNGVIQQEIKYNNGKPDLSRRFDFM
                     GRLEMEENYKDGLLNGYVRQYTSYQKKIMEAFYVDDTLHGDYFEWHENGIPKVIGHYD
                     KGLFDGKWEFFDENGLKLGEGNFYKGDGVQKSWYPNGAMQKIIHYKNNRKEGLEQEFS
                     LEGKPIREINYHHDSIIDVKNFEINP"
     gene            3008..4342
                     /gene="gdhA"
                     /locus_tag="PLF20_03365"
     CDS             3008..4342
                     /gene="gdhA"
                     /locus_tag="PLF20_03365"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOX80081.1"
                     /translation="MNLEKIMFDLEKRNPGQPEFLQAVHEVLESIKEFVNKNPKYDSY
                     KIIERLTEPDRILQFRVTWVDDNGEIQVNRGYRVQFNNAIGPYKGGIRFHPSVNLSTL
                     KFLGFEQIFKNSLTTLPMGGAKGGSDFNPKGKSENEIMRFCQAFMMELWHNIGPEMDV
                     PAGDIGVGGREVGYMYGCWKKLTREHNGVFTGKGRNWGGSLIRPEATGFGVVYFANEM
                     LKLMGKDIKGKTIAISGFGNVAWGAVTKANHLGAKVVTISGPDGYIYDPDGISGDKID
                     YMVELRASNNDIVKPYAEKYGVEFFEGRRPWEVKCDVAMPCAIQNELDEKDARNLINN
                     GVTIVCEAANMPCTPEAINLFHQHRIPFGPGKAANAGGVATSGLEMSQNSMKLNWSAA
                     EVEERLREIMINIHDNCVKYGKEPDGYINHVKGANIAGFIKVADAMIDQGVY"
     gene            4578..6038
                     /locus_tag="PLF20_03370"
     CDS             4578..6038
                     /locus_tag="PLF20_03370"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOX80082.1"
                     /translation="MNILLKQQYTSEDFNTHCAFLFPSLEDIPEKWLSTQELNFVKRV
                     NTNSPDETVIPVHRANYSWFCILPKPNENPAKKLENIRLAGYQLTQFLHKERIDELHI
                     SSFHASSLDLFALAEGIVLSSYAFQKYQNNEKKRKHQLQTLDILSENFDYKQLKELEI
                     ITEAVYLCRDLVNEIPSELNAKSLAEILKQKAEEVGIRAEVMNKKKIESLKMGGLLGV
                     NKGSTIPPTFTILEWKPFNFINEKPIILIGKGITFDSGGMNIKTGTYMENMKSDMAGA
                     ATVASVVIAAARLSLPLNVMAFVPATDNRINSDAMVSGDILTMYNGKTVEVVNTDAEG
                     RLILADALSYANKFDPFLTITAATLTGAAARAIGKYGIVAMESHARTQFDLLKQSGEE
                     VYERLVEFPLWEEYFDLIKSDVADIKNSGPAEAGAITAGKFLEYFTTYPFIHLDIAGT
                     SFTNKNDGYRGIGATGIGVRLLISFLQNVIQNHQNS"
     gene            6040..7164
                     /gene="pdxA"
                     /locus_tag="PLF20_03375"
     CDS             6040..7164
                     /gene="pdxA"
                     /locus_tag="PLF20_03375"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOX80083.1"
                     /translation="MDNDILNLDPYENSDLQEPEMIRIGFTQGDINGIGYEIIMKALS
                     EIGTPYSFIPILYGSSKIASYHRKTLSIPDLPFNHVRKASQALPHKVNIVNITDKEIR
                     VNLGQSTPEAGEMAYLSLHQACRDLEDGLLNVLVTAPINKYNIQSPEFHFPGHTEFLA
                     NRFRAANPIMTMVYNNLRIGFVTNHVPVRQIADNITTDLVYNKIKALNLSLFQDFGIS
                     HPKIAVLSLNPHAGDRGLLGSEENEIISPAIHQAFEEGILALGPFPADGFFGMDHYRK
                     FEGILAMYHDQALPVFKILAQDYGVNFTAGLPFVRTSPAHGTAYDIAGKDKASHHSMR
                     EAIYLAIDVFKNRLEYARITANPLRSPIQQNSEETEEEEI"
     gene            7179..9656
                     /locus_tag="PLF20_03380"
     CDS             7179..9656
                     /locus_tag="PLF20_03380"
                     /inference="COORDINATES: protein motif:HMM:NF008897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     UDP-N-acetylmuramoyl-tripeptide:D-alanyl-D-alanine
                     ligase/alanine racemase"
                     /protein_id="HOX80084.1"
                     /translation="MDHTISSLATIVTPIWVSLTKPDDMIRFLLTDSRRLVEAKSTLF
                     FAITTSRNDGHHYVEDLFKKGVRNFVVTNTQLFPNLLGHANFIQVENTINALQKIAAA
                     HRAEFQIPILGITGSNGKTIVKEWLTQMILTKLCVCASPDSYNSQIGVPLSVWQLNDY
                     HQLGLFEAGISQPHEMESLQKIIKPNYGIFTNIGQAHSENFGTLEEKTIEKTKLFRET
                     EVLIYCRDHELIHKVVKENLKSKTKLITWGENPESTIRIIQKTRNGKTTSLTLVNNQQ
                     KIQLELPVSDMASIENAMHCYTFLDCLGWDDKTKAEAILQLHPIPMRLEMMEGINQCT
                     LINDTYNSDINSLEVALDYLNRQAGVRPTAVILSDILQSGQNPAELYDHVAYLLNLRN
                     VGRVIGIGSEISKFGGLFPMQKNFYVSTQQFIDEFPFSSLQNDVILLKGARSFEFENI
                     TRILRRRSHQTVLEINLENLIHNFNFYKQKTKPGTLTLAMVKAFGYGSGMHEIASILQ
                     QNSVDCFGVAYPDEGAQLRRAGIQTPIMVMNPENDNLDIITQFHLEPEIYSQTSLTQL
                     AHYLSSRRPDYLPFPIHLKFDTGMHRLGFLPEQLDEALMFLKQHPNIKVKSVFSHLAA
                     SEDPLMDDFTRKQAEIFEEMAQKTIRTLEYPVIRHLVNSAGIIRFPEYHFEMVRIGLG
                     LYGIGHDEKTQRSLQLVSKFSSVISQIKTIRKGEGIGYNLKWRAPADTRIGIVPVGYA
                     DGLRRELSSKGFCFHVRGHLAPIIGAISMDMCAIDLTGIAATEGDPVIIFDNADTIHQ
                     LSRLLNTIPYEILTSISQRVRRVYLMG"
     gene            9669..11969
                     /locus_tag="PLF20_03385"
     CDS             9669..11969
                     /locus_tag="PLF20_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOX80085.1"
                     /translation="MDQITLSIIIPAYNEEKTIVKVIHQVWETPLQENVSREIIIVDD
                     GSKDATVKVVKEFLDQNSEKNIRLIECGKNRGKGAAIREGIKQACGKWIIIQDADMEY
                     NPRDYNILLQPALEGYADVVYGSRFVGGNPHRILFFWHSIGNKFLTFISNAFTNLNLT
                     DMETGYKLFRADIIKSINLEENRFGFETEVTAKIARIPGIRIYEVGIAYYGRTYAEGK
                     KIKAKDGFRALYTILKYNLQSKNPRRPIALVITLLLLFIVGLKINDYYKDKPFNFTRN
                     DAGEYYQYLPALFIHHDLGFGFLEKTTPDKYEHYYKFSAYRFENGRSTGKMSLGMAMI
                     LTPGFLIAHGIAILTGLPADGFSPPYQWAILLTNLLFLGLGIYFTLKTLFRYFDSITS
                     VITIVMLVLATNVLAYVYLHPGFSHIFTFGLMGMLLYVTDTFYRKPSIGLAMKAGLLM
                     GFISLIRPSNTIVGLLFLLWGTFSVSDLKKRFQFFIKNYHLTLIIAAGIVIVWLPQMI
                     YWKWVSGRFFLYTYGLKGERFFFGNPQIVNVLLSYRNGWLVYSPLFFLALLGFIFMIK
                     NKNRQVLAIGFYFIGLIYIISSWWCWWYGGSFGARSLIDAYPALAFPLAAFFEKAGKT
                     PWWLQILLIIVCGYLIHLNYYQSYQYSKGIIHWDGMSKEAYWRSIKEKHPPKDYYDLF
                     VIPHTPYAKKGIYFEHDTLNIQRPPSLQEKEFIEKIKTNIRINKEWMQVIEEKAKRKG
                     ISVDSMLTLDARHIYKIRYHEKVPKN"
     gene            11989..13398
                     /locus_tag="PLF20_03390"
     CDS             11989..13398
                     /locus_tag="PLF20_03390"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOX80086.1"
                     /translation="MTFNSLPFLIFLPIIVITYYLLSPKWRWIMLLAASYLFYMWWNP
                     AYIILILVSTLISFYSSQAIERQTSKKGKKKYLYLCLIINLGILFTFKYYGLFSQAFT
                     SIADIFGVKAQLPFLNLLLPVGISFYTFQTLSYTLDVYFGKQKAETHLGYFALFVTFF
                     PQLVAGPIERYDRLSPQLKSPHHPSYENFSNGLRLILFGLFIKMVIADNLAPYVEEIY
                     SQPSAYNTYHLLAGLGFYSFQIYSDFYGYSTIALGSALLLGVNLMDNFKTPYLSANIR
                     EFWRRWHISLSTWFRDYVYIPLGGNRVKWSRWALNIFIVYTLSGLWHGANWTFLIWGT
                     IFGLMFIIERFCYERFGITSTQKWGIRRIFGVIITFTIVTLAWIFFRSSSLSNALTMF
                     EALWNNTALADSWRPDPFIMFWLSLFILLDILLFNNRFDKWCSKKPLWLRWTIYTLLI
                     FGITAFSGIDNQPFIYFQF"
     gene            13398..14927
                     /locus_tag="PLF20_03395"
     CDS             13398..14927
                     /locus_tag="PLF20_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80087.1"
                     /translation="MNSLATKILPRLFVLAIIAFLLNWIYSVAFYPSDLKKIDLIDSL
                     QKVIPRSDVIYFGESSNFSFVFEDDDQRRISDFISDYYPGLQFGTVNKGALHAGIYLK
                     LLKQIPTEAPVNTIILSMNLRSFSADWIYSKLESLLQRDMVLISDGPPLWNRFRLGLK
                     AYDNKTQKQRSMQVKNALKKDLLFLEPPCDFPNAVAWIENINNSPTSPWIEDSLAKSL
                     AISFIINYGFRIDTVNNPRIRDFDKIIEYARQRGWKVVLNILPENTQQASLLIGPCLR
                     KLMISNAKLLTKRYESQGAIVVNNLELVDSSHYIDKKWPTEHYDEYGRKMVARQIALA
                     MRNIYPAHFSDVFMKSDFTCDFEAPSPFFVTLGRDSTCSHSGKFAEKLDTNNAFGARF
                     VRHYFEVKTLNPRKITASGWFTGIYRPREVKLIISIENDKKQPKLWTATVSKDIIEYG
                     KWDSLQVNANIPENFGDNDVIRIYFWYTGKNKVWIDDVNILLQTENLNAASDTILLIK
                     N"
     gene            14997..16850
                     /locus_tag="PLF20_03400"
     CDS             14997..16850
                     /locus_tag="PLF20_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80088.1"
                     /translation="MKKIKHPISAASLLLCVIVLISVRLSHPPKNILSYDTFGYYIYL
                     PARHIYHDPGIHNFEWVKEINQKYNNTPTFYQFSEGINGQKVIRFNRGISYLLAPGFY
                     VGHAWAKLSGAPQDGFSKPYQQAIWIWGMIFNLLGFYLLKKILLRYFNDLTTGVTLIV
                     LLLISNLYFFYGYGSDIPHVYLFTLNAALIWFTIHWHHHLKIWDAALIGLTLGMIAIS
                     RISEVLIVFIPLLWGVKNKETLKSKFSLFLNKWPHVLVAIIAGIIPLLSQIIYWKSVS
                     GEYLYQTYNDPGSTLDLMNPRFFHTLLSFRKGWLIYAPVMILALWGIYKAFRKKEEWA
                     WAILVYIALDIYVISSFTSLLSYGWRAFLQSYAALVLPMGVFIQWMLSKKPLTIVGWC
                     MILVILGVINIFQAWQINMGIIDGSRMTMKYYVSVFMKKHPPENAKKYLLVQRSATGR
                     EDLKETESYFNHVLKFYDFETPNPKLQSHIDSIVSFSGRYCLRLDSTIEFTPGLECTY
                     GDISNRKWVWIRLSARVYPAQPLEGSSVLLVTHAIRNGQAYKYTARKIADTLFHLKPN
                     QWNYVWHDYMTPEPISAEDGIKSYIWNRNKNPVFVDDIRIEVFEPLSSIIE"
BASE COUNT         5345 a         3584 c         3303 g         5004 t
ORIGIN      
        1 ggataatttt tctatttgtc aagtattttt aaactgacaa cgaacaacca acaatctcca
       61 atctccaacc cccaatctcc aatctccaat ccccaatctc caacctccaa tctctattct
      121 cccttcctcc attcctctgt tttcccttcc tttagttaac atcaggaaaa ttttttctcg
      181 ctgatttccg cagattaatc cgctgattta cgtaaattac aactaacaac tgataactta
      241 gaaattttaa attacaaaca acctccaatc cccaaccccc aacctccaat ctccaacctc
      301 caaattccaa tccccaaccc cattctctat tctccctttc tccgttttta tctttttctg
      361 acttatttta atcggcatga ttcctaatgt cattttgcag aaatgatgat cagagactcg
      421 tgaaatgcaa tctcataaaa tagctctaat aaatacaatg gcaagtgcta tgacgaaaat
      481 catcttcatt agagtactgg taataaaacc aaaaaaagag cccagacccg agcgcaggcc
      541 atagttcaaa ttcttaccac ccaaaagttc accaatcaat gctcctaaaa aggggcctat
      601 gatgattccc agaggcccaa ataaaaaacc gatcaccagc cctatggcac tgcctcgggt
      661 tccatatttc gtgcctccaa attttcgggt tcctataatg ggtatgacat agtccaacag
      721 agtaatcagt atggtggcaa ttccccataa aatcaaggtt ttttgtgaga aaggagaaaa
      781 agaggtgagt tgaagtgcag gtaaagccaa ataggctaaa ggcgggccgg ctaaaaaggg
      841 agcaaaacta cctataaaac ctaaaaccaa taaaatagct ccgatagtta tccaaatcca
      901 atccattttt taacagtata caaagattca aatgtaatac ttttgcgtaa gaaaaaataa
      961 aatctttggg tgctgaatta tttattgctc gtcgtttaat taagcctaaa aaggaaagtc
     1021 tttcagggcc ttcggtgagg attgccatag caagtgtaag cataagtctt acggtgatga
     1081 tttcttcggt ttgtatatta agagggtttc agcaggagat aagggaaaag gtaagtggat
     1141 ttgcggctca tatacaggtt cattcctttg ttagtggagt cagccaaagt gaaaatccac
     1201 ccatgactta cgacaccggg ctcataatga aactaagaaa ttatcctgga ataagacatg
     1261 ttcaggcagt agcttataaa actggcatga ttcaagctaa tcaacaaatt ttaggtataa
     1321 ccctgaaagg cggaggtccc gatctcgata tgcaattgat caaagacaaa ctcgtgaaag
     1381 gaagattgcc tttgatgggt gatactgctt tgcgatatga aattctcatt tcgcaaaata
     1441 tggcagataa gttacttctc gacactggac aagatatccg catgtatttc ttgaatgaag
     1501 gagaacgttc accccgagca cgcaagatga agattgtggg catttataat accggcctcg
     1561 aggattttga tcagtcgttt gcctttaccg atattcgcca cattcaaaga ctcaatggat
     1621 ggacccacaa agaaatagga atgatagaaa tcatccttcg aaatattgat gagttggata
     1681 tgataagccg taaggtttat gaagatttac cttctcatct caacgtaaac aacattcggc
     1741 agatctatcc tcaaattttc gattggctcg agctgcaaga tgtaaatgta ctggtcatcc
     1801 tgattttaat gataatagta tccgccattt cgatgataag cgctcttttt atcatgatag
     1861 tagaaaaaac tcaactcatt ggtattttaa aggctttagg agcaactaat ttaagcatta
     1921 gaaaaatttt cattcagact tcctcgttta tagccttaaa agggatggtc tatggaaatg
     1981 tgataggcat tggccttatg tggcttcaaa agcatcttca tctggtaaaa ctgcctgtag
     2041 aatcgtattt tatggagtat gtacctgtaa gtcttagttt gacaaacatt ctcatcatca
     2101 acataacaac tttaatttta tgcataagtt cacttcttat acccacttat accgttactc
     2161 gagtctctcc cctaagagcc attcgatatc attgaaaccg ttcctaaaaa tttgtgtgat
     2221 tttttgcata gttctcctat taggaagctg taaaaaaaaa gaaacgaatt attaccacga
     2281 tggcaaattg atgtcggaaa tagaaatgaa ggggaaaaag aaacacggga aggctattta
     2341 ttactatcct aatggagtga tccagcaaga aataaaatac aacaatggga agcccgactt
     2401 atcgagacga ttcgacttca tggggaggtt agaaatggaa gaaaattaca aggatggatt
     2461 gctcaatgga tatgtgcgac agtacacttc ctaccagaaa aaaattatgg aagcctttta
     2521 cgtcgatgat accctgcatg gagactattt cgaatggcac gaaaatggaa tacctaaagt
     2581 aatagggcac tacgacaaag gcttatttga tggaaaatgg gagtttttcg atgaaaacgg
     2641 tttaaagctg ggtgaaggaa atttttacaa aggtgatgga gtgcagaaat catggtatcc
     2701 taatggggcg atgcaaaaaa tcattcatta caaaaataat cgtaaagaag gtttagagca
     2761 agagttttct ttagaaggaa aacctataag agaaataaac taccaccatg atagcataat
     2821 cgatgtcaag aatttcgaaa taaatccata aagactactc gtgaagatac atatatatta
     2881 gaacccttct aaaatacgat aaaagtttga cggaagaaat ataagccata attttgcatt
     2941 gtgaaataat taacaatgtt tattcacaaa cttgaaattt ttaaaaccct aataaaaaca
     3001 aattgatatg aatttagaaa aaattatgtt cgatctggag aagagaaatc caggacagcc
     3061 tgaatttttg caggcagtac acgaagtgct cgaaagcata aaggaatttg taaataagaa
     3121 cccaaagtat gattcttata aaatcatcga gcgtttaacc gagcccgatc gtatccttca
     3181 gttccgtgtt acttgggttg acgataacgg agaaattcag gtaaacagag gatatcgtgt
     3241 gcagttcaac aatgccatag gcccatacaa gggaggaatt cgttttcatc ccagtgtgaa
     3301 cctgagtact ttaaaattct tgggtttcga gcagattttc aagaatagcc tcactacctt
     3361 accgatgggt ggagcaaaag gtggcagcga tttcaatccc aagggcaaga gtgaaaacga
     3421 gattatgcgt ttttgccagg cctttatgat ggaattatgg cacaatattg gccccgaaat
     3481 ggacgtacct gctggtgata ttggcgtagg tggccgtgaa gtcggataca tgtatggctg
     3541 ttggaaaaaa ttaaccagag aacacaatgg agtattcacg ggtaagggcc gcaactgggg
     3601 tggctcgttg atccgtcccg aagctactgg atttggtgtc gtttattttg ccaatgaaat
     3661 gttaaaactc atggggaaag acattaaagg gaaaacaata gcaatttcgg gatttggcaa
     3721 tgttgcctgg ggagctgtaa ccaaagccaa tcacttgggt gcaaaagtcg ttaccatatc
     3781 tggaccagat ggatatattt atgatcctga cggaatttcg ggagataaaa tcgattatat
     3841 ggtcgaactc agggcttcta acaatgacat tgtaaagccc tatgctgaaa aatacggagt
     3901 cgaattcttc gaaggtcgtc gtccatggga agtaaaatgt gatgtagcta tgccttgtgc
     3961 cattcagaat gaactcgacg aaaaagatgc tcgtaatttg atcaataatg gtgtgaccat
     4021 tgtttgcgaa gctgctaaca tgccatgtac acccgaagcc atcaatcttt tccatcagca
     4081 cagaattccc tttggacctg gaaaagctgc caatgctggt ggcgtagcta cgtcgggatt
     4141 ggagatgagc cagaacagta tgaaactcaa ctggtcggct gccgaagtag aagaacgcct
     4201 ccgtgaaatc atgatcaata tccacgacaa ttgtgtaaaa tacggcaagg aacccgatgg
     4261 ttatattaac cacgtaaaag gtgccaacat tgcaggattc atcaaagtgg ccgatgcaat
     4321 gatcgaccag ggagtttatt gataatagaa ttttcttttt ctccgtggga agccgcctcg
     4381 aaatttttcg gggcggcttc ccacattttc cccagaacac ctcccctccg ttaattcatt
     4441 aaaataactg tatttatcct tccactgaaa tcaaccaccc actcattcat attcagcctc
     4501 tgccagggat cggaaaaaga aattatgaaa gtttatccga attcctttaa ctttgcgcac
     4561 tagaagcaat aaacccaatg aatatcctgc taaaacaaca atatacctct gaggatttca
     4621 atactcattg tgcatttctt ttcccatcac tcgaagatat tcccgaaaaa tggctgagta
     4681 cgcaagaatt gaactttgta aaaagggtaa atacaaactc acccgatgaa acggttatcc
     4741 ctgttcaccg agccaattat tcatggttct gtatcttgcc taagccaaat gaaaacccag
     4801 cgaaaaaact cgaaaacatc cgactagcag gttatcagct cactcaattt ttacataagg
     4861 aaagaatcga cgaactgcat atttcttctt ttcatgcttc ttcacttgat ctatttgcat
     4921 tggccgaagg catcgtatta agtagctatg cttttcagaa atatcaaaat aacgaaaaaa
     4981 agagaaagca ccaattgcaa accctcgata ttttatcaga aaatttcgat tacaaacaac
     5041 tgaaagaact cgaaatcatt actgaagctg tttacttatg tcgcgatttg gtgaatgaga
     5101 ttcccagtga attgaacgcc aaaagtctgg ctgaaattct aaaacaaaaa gccgaagagg
     5161 ttggcataag agccgaggtt atgaacaaga aaaagataga atctttgaaa atgggaggct
     5221 tactgggagt aaacaaagga agtacgatac cgcccacatt caccatactc gaatggaaac
     5281 cttttaactt tataaacgaa aaacccatca ttctaatcgg caagggaatc actttcgata
     5341 gtggaggtat gaacatcaag actggcactt atatggagaa tatgaaatcc gacatggcag
     5401 gtgccgccac tgtagcttcc gttgtaattg ccgcagccag actatcattg cccctgaatg
     5461 tcatggcatt cgtcccagcc accgacaatc gcatcaattc cgacgccatg gtctcgggag
     5521 atattcttac gatgtataac ggaaaaacag tagaggtggt gaacaccgac gccgaaggaa
     5581 gattgattct ggccgatgcc ttaagctatg ccaataaatt cgatcctttt cttacgatta
     5641 ctgcggccac actcacaggt gcagccgcca gagccattgg taaatatgga atagtggcaa
     5701 tggaatcaca tgccagaacc cagttcgact tgctcaagca aagtggagaa gaggtatacg
     5761 aacggctggt agaatttccc ctatgggaag aatatttcga cttgataaaa tccgacgttg
     5821 ccgatataaa aaattcaggg ccagccgaag ctggagccat caccgcaggc aagttcctcg
     5881 aatattttac cacttaccct ttcattcacc tcgatattgc aggaacttcg ttcaccaata
     5941 aaaacgatgg atatagggga attggagcca ccggaatcgg tgtgagactg cttatatcct
     6001 tcctgcaaaa tgttattcaa aaccaccaaa attcttaata tggataacga tatcttgaac
     6061 ctcgaccctt atgaaaattc cgatcttcag gagccggaaa tgattcggat tggatttact
     6121 caaggcgata tcaatggaat tggctatgaa ataattatga aagctctttc cgagattgga
     6181 acgccctata gttttattcc tattctgtat ggctcatcca aaatagcttc ctatcaccga
     6241 aaaaccctca gcatacccga tctgccattc aatcatgtac gtaaggcttc tcaagccctt
     6301 cctcacaaag taaatatcgt caatattact gataaggaaa tcagggtaaa tttaggacaa
     6361 tcaacacctg aagcaggtga aatggcctat ctctcactcc atcaggcctg ccgtgacttg
     6421 gaagatgggc tattgaatgt tttggttact gctccgatta ataagtacaa tattcaatct
     6481 ccagagtttc atttcccggg acatacggag tttctggcca accgctttcg tgctgccaat
     6541 cccatcatga ccatggtata caataatcta aggatagggt tcgttaccaa tcacgttcct
     6601 gtgcgtcaaa ttgccgataa tatcactacc gacctggttt acaataaaat aaaggcattg
     6661 aatttatcct tatttcagga ttttggcata agccatccta aaattgctgt attaagcctt
     6721 aatccccatg ctggtgatcg cggattactc ggcagcgaag aaaatgaaat cattagcccc
     6781 gccattcacc aagcattcga agaaggtatt cttgctcttg gaccttttcc tgcggatgga
     6841 ttttttggga tggaccacta tagaaaattc gagggaatat tggccatgta tcatgatcaa
     6901 gcattacccg tattcaaaat tctagcccaa gactatggag taaattttac ggctggactt
     6961 cctttcgtaa gaacatcacc cgcccatggc acagcttacg acattgcagg gaaagacaaa
     7021 gcctcgcatc attccatgag agaagcgatt tatctggcta ttgatgtttt caagaaccgc
     7081 ctcgaatatg cccggataac tgccaatcca cttcgttcac ctattcagca aaattccgaa
     7141 gaaaccgaag aggaagaaat ataactcaca ggtcagcaat ggatcatacc atttcatcac
     7201 ttgcaaccat agttactcct atctgggttt ccttgacaaa gcctgatgat atgatacgat
     7261 ttctgctgac agatagccga cgattggtag aggcaaaatc tacacttttt tttgctatta
     7321 ctacatcacg aaacgatggc caccactatg tagaagatct tttcaaaaag ggagtaagaa
     7381 atttcgtcgt caccaatact caactcttcc caaatttatt aggacatgcc aattttatac
     7441 aggtagagaa taccataaat gcattacaaa agattgctgc tgctcaccgg gccgaatttc
     7501 agatccctat acttggaatc acaggaagta acggaaaaac aatagtgaaa gaatggctca
     7561 cccagatgat tttgactaaa ctttgcgttt gcgctagtcc cgatagctac aactctcaaa
     7621 ttggtgtacc tctttcggtt tggcaattaa acgattacca tcaactggga ttattcgagg
     7681 cagggatttc tcaaccccat gagatggaat cacttcaaaa aattattaaa cccaactatg
     7741 gaatttttac caatattgga caagctcatt ccgaaaactt cggcaccctc gaagaaaaaa
     7801 ccatagaaaa aacaaaactc ttccgggaaa cggaagtgct gatctattgc agagatcatg
     7861 aactgattca taaggtagtg aaggagaatc tgaaatcaaa gacaaagctc attacctggg
     7921 gagaaaatcc ggaatcgaca attcgaatca tccaaaaaac aagaaacggc aaaactactt
     7981 cccttacatt ggtcaataat caacagaaaa tacagttgga attaccggta agcgatatgg
     8041 cttcaataga gaatgccatg cactgttata ccttcttaga ttgtttagga tgggacgata
     8101 aaacaaaggc cgaggccata cttcagcttc atcctattcc catgcgactg gagatgatgg
     8161 aaggaataaa ccagtgcacc ttgattaacg acacttacaa cagcgatatc aattctcttg
     8221 aagttgccct cgattatttg aaccggcaag caggcgtccg tcctacggca gttattcttt
     8281 cggacatatt acagagcgga caaaatcctg ctgagcttta cgaccatgtt gcttatctac
     8341 tgaatttgag aaatgtggga cgggtaatag gtataggttc tgagatttcg aaatttgggg
     8401 gtttattccc aatgcaaaaa aatttttacg tttccactca acagtttatt gatgaatttc
     8461 ccttctcgag cctacaaaac gatgtcattt tgttgaaggg tgcacgatcg ttcgagtttg
     8521 agaacatcac ccgaatatta cgtcggcgtt ctcatcaaac tgtgctcgaa atcaatctgg
     8581 agaaccttat tcacaatttc aatttttaca aacagaaaac caagccgggt acattaacct
     8641 tggcaatggt caaagccttt ggatatggca gtgggatgca cgagatagcc agtattctcc
     8701 agcaaaatag tgttgactgt tttggtgtgg cctatcccga cgaaggtgct caacttagaa
     8761 gagctggcat tcaaaccccg ataatggtta tgaatcccga gaacgataat ctcgatatta
     8821 tcacccaatt tcatttagaa ccagaaattt atagtcagac ctcattgacc caactggcac
     8881 attacctgtc ttctcgacga cccgattatc tgccattccc aattcatttg aaattcgata
     8941 caggcatgca cagattggga tttttacctg aacaactcga cgaagccctc atgtttctta
     9001 aacagcaccc taacataaaa gtaaaatcgg tattttcgca ccttgccgcc agcgaagacc
     9061 cacttatgga tgacttcacg cgaaaacaag ccgaaatttt cgaagaaatg gctcaaaaaa
     9121 caataaggac tttggagtat cctgtaatca ggcatttggt caattcggcc ggaattattc
     9181 gatttccgga atatcatttc gagatggtac gcatagggct cggattgtat ggaataggcc
     9241 atgatgaaaa aactcagcgc tcactccagt tagtaagcaa attctcttca gttatctctc
     9301 aaattaaaac cattcgaaaa ggcgaaggaa tagggtacaa tctgaaatgg agagcaccag
     9361 ccgacacccg catcgggatt gtccctgtgg gttatgccga tggcttacga agagaattat
     9421 catccaaagg attctgcttc cacgttcgtg gtcatttggc tcctattatt ggtgccatct
     9481 ctatggatat gtgtgccatc gacttaaccg gcatagccgc aacagaaggc gacccggtta
     9541 tcattttcga caacgcagat acgattcacc aactttcccg tttactaaat actattccct
     9601 acgaaatatt gacttctatc tctcaacgag ttagaagagt atatttaatg ggataaaatt
     9661 aatttgatat ggaccaaatt acactgagca ttatcatccc tgcttataat gaagaaaaaa
     9721 ccatagtaaa agttattcat caagtttggg aaacacctct acaagaaaat gttagccgcg
     9781 aaatcatcat agtggatgat ggaagtaaag atgcaacggt aaaagttgta aaagaattct
     9841 tggatcaaaa tagtgagaag aatatccgac tgattgaatg tggtaaaaat cgtgggaaag
     9901 gcgctgccat tcgtgaaggc ataaaacagg cctgtggcaa atggatcatt attcaggatg
     9961 cagacatgga atacaatcct cgcgattata atatcttgct tcaacctgcg cttgaaggat
    10021 atgcagatgt agtttacggg tcgagatttg tgggaggcaa cccacaccgc attttatttt
    10081 tctggcattc tatcggaaat aaattcctta ccttcatttc gaatgctttt accaacctaa
    10141 atctcaccga catggaaacc ggctataaac ttttcagagc tgatatcatt aaaagcatta
    10201 acttagaaga aaatcgtttt gggtttgaaa cggaggttac cgcaaaaatt gctcgaatac
    10261 ctggcattcg aatttatgag gtagggattg cctattatgg ccgaacctat gccgagggga
    10321 aaaaaataaa ggcaaaggat ggattccggg ccttatacac tattctaaaa tataacctcc
    10381 aaagcaaaaa tcctcgacgc cccattgcct tggttattac cttgcttttg ttattcatcg
    10441 ttggtttgaa gatcaacgat tattataaag acaaaccctt caattttacg aggaatgacg
    10501 caggtgaata ttatcaatat ttacccgctc ttttcattca tcatgatctt ggatttggat
    10561 tcctcgaaaa aaccacaccc gacaagtacg aacattatta taaattttcg gcctatcgct
    10621 ttgaaaatgg gagatccaca ggcaaaatga gcttgggcat ggctatgatt cttacgccag
    10681 gttttctcat cgcccatggc atagccatac ttacgggctt gcctgccgat ggattttcac
    10741 ctccctatca atgggcaatt ctcctgacta atttactttt cttgggactg ggaatttatt
    10801 ttacactcaa aaccctgttt cgttacttcg acagtattac ctcggtgata accatcgtta
    10861 tgttagtgct tgccactaac gtattggcgt atgtttatct gcatcctggc ttttcgcaca
    10921 tctttacttt cggactgatg gggatgctct tgtacgtaac cgataccttc tatcgcaaac
    10981 catcaattgg attggcgatg aaggcaggct tgctcatggg ttttatcagt ttgatacgac
    11041 ccagtaacac cattgttgga ctgcttttcc tactttgggg gacattttcg gtgagcgacc
    11101 tgaaaaaaag attccagttc ttcataaaaa attatcatct aacattgata atcgctgctg
    11161 gaattgttat agtatggctg cctcaaatga tttactggaa atgggtaagt ggcaggtttt
    11221 tcttatatac ttatggattg aagggtgaaa gattcttttt tggtaatcct caaatcgtca
    11281 acgttttact aagctatcgc aacggatggc tggtttattc acccctgttt ttccttgctt
    11341 tgcttggttt tatttttatg ataaagaaca aaaaccgaca ggttttagcc ataggttttt
    11401 attttattgg acttatatac attatttcat cgtggtggtg ctggtggtat ggaggttcat
    11461 ttggagctcg tagcttgatc gatgcatacc ccgctctggc tttcccatta gccgccttct
    11521 tcgaaaaagc agggaaaacc ccctggtggc ttcaaatatt gctcattatt gtttgcggtt
    11581 atctcatcca tctgaattac taccagtctt atcaatattc aaaaggaatt attcattggg
    11641 atggaatgtc gaaagaagca tattggagat ccataaaaga aaagcatcct ccaaaagatt
    11701 actacgattt atttgtaatt ccccataccc cttatgccaa gaaaggaatt tatttcgaac
    11761 acgatacgct gaatattcaa agacccccat ctttacagga aaaagaattc atcgaaaaaa
    11821 taaaaaccaa tatccggatt aataaagaat ggatgcaagt tatagaagaa aaagccaaac
    11881 gaaaaggaat atctgttgat tctatgctca ctttagatgc tcgacacatt tacaaaatca
    11941 ggtatcacga aaaagttcct aaaaattaaa aaaaattagg aattcaacat gacatttaat
    12001 tcccttcctt ttcttatttt tctgcccatt atcgtcatca catattatct tctatcacca
    12061 aagtggcgat ggataatgct acttgcggcc agctacctgt tttatatgtg gtggaatcca
    12121 gcttatatca ttttaattct tgtttcaaca ctgattagtt tttattcgag ccaagccatt
    12181 gaaagacaaa cctcaaagaa agggaaaaaa aaatatcttt atctatgctt gattatcaat
    12241 ttaggaattt tatttacttt taaatattac ggattatttt ctcaagcctt cacgagtatt
    12301 gccgatattt ttggggtgaa agcccaactg ccttttctga atttattgct tccggtagga
    12361 atttcatttt acaccttcca aaccttaagc tatacattgg atgtatattt tgggaagcaa
    12421 aaggcagaaa ctcatctggg gtatttcgct ctttttgtga cttttttccc tcaactggtg
    12481 gccggaccaa ttgaacgcta cgacaggctt tcacctcaat taaaaagccc acatcatcct
    12541 tcttacgaaa acttttctaa cggcttaaga cttatccttt ttggattgtt cattaaaatg
    12601 gtcattgccg ataatctggc tccttatgta gaagaaattt acagtcaacc ctcggcttac
    12661 aatacatatc accttttggc aggacttgga ttttattcat ttcagattta ttctgatttt
    12721 tatggatatt cgaccatagc ccttggctct gctttacttc tgggagtcaa tttaatggat
    12781 aatttcaaga ccccctattt atcggcaaat attcgggaat tctggagacg ctggcatatc
    12841 agcctaagca catggttccg agattatgta tatattcctc ttggaggcaa tcgtgtaaaa
    12901 tggagcagat gggcacttaa tatctttatc gtttacacac taagcggatt atggcacgga
    12961 gctaactgga cattcttgat ctgggggact atcttcggat tgatgttcat catcgaaaga
    13021 ttttgctacg aacgctttgg aattactagt acgcaaaaat ggggcatcag aagaattttt
    13081 ggtgtaatca taacttttac catagttacg cttgcctgga tttttttccg cagcagctct
    13141 ttatcgaatg ccctgaccat gttcgaggca ttatggaaca acaccgcatt agccgattct
    13201 tggcgacccg atccttttat tatgttctgg cttagcctgt ttattcttct cgatatactc
    13261 ctcttcaata accgttttga caaatggtgt tcaaaaaaac cattatggtt gcgatggact
    13321 atctatacct tgctcatttt cggcattaca gcctttagtg gaatcgataa tcaacccttt
    13381 atatatttcc agttctgatg aattcgcttg ccacgaaaat attgccccgc ctgttcgttt
    13441 tagccattat tgcctttcta ttgaactgga tctattctgt tgcgttctat cccagcgacc
    13501 tgaaaaaaat cgacctcatc gattctttac agaaagttat acctcgatca gatgtcattt
    13561 atttcggaga atcctccaat tttagttttg tttttgaaga cgacgaccaa cgccgcataa
    13621 gtgattttat atccgattat tatcccggcc tgcaatttgg cacagtcaac aaaggagcct
    13681 tgcacgccgg tatttacttg aagctgctga aacaaattcc cacggaggct cccgtaaaca
    13741 caatcattct gagcatgaac ctccgttcgt tttcggccga ttggatctat tcgaaactcg
    13801 aaagcctgtt gcagcgcgat atggtattga tcagcgatgg gcctcccctt tggaatcgct
    13861 tccgactggg actcaaagcc tacgataata aaacccagaa acaacgtagc atgcaggtta
    13921 aaaatgcctt aaaaaaagac ctactcttcc tcgagcctcc atgcgatttt cccaatgcag
    13981 tcgcatggat cgaaaacatc aataattcgc caacttctcc atggatcgag gatagtttag
    14041 caaaatcact ggcaatttca ttcatcatta attatggttt tcgaatcgat acagtcaata
    14101 atccaagaat acgcgatttc gataaaatca tagaatatgc ccgccaaaga ggctggaaag
    14161 tagtgttgaa tattttaccc gaaaacaccc agcaggcttc tttattgata gggccgtgcc
    14221 ttagaaaact tatgatcagc aatgcaaaac tgctcaccaa aagatatgaa agccaaggcg
    14281 caatcgtagt caataatctc gagttggtag attcttcaca ctacatcgac aaaaaatggc
    14341 ctaccgagca ttacgatgaa tatggtcgaa aaatggtagc ccgtcagata gctctggcca
    14401 tgagaaatat ttatcctgcc catttttccg atgtattcat gaaatcggat ttcacttgcg
    14461 atttcgaagc cccatcacct ttttttgtta cacttggaag agattcgact tgctctcact
    14521 caggaaaatt tgccgaaaaa ctcgatacca ataacgcatt tggagccaga ttcgttagac
    14581 attatttcga agtgaagact cttaaccctc gaaaaattac cgccagcgga tggtttacgg
    14641 gcatctatcg gcctcgtgaa gtaaaactca tcatcagcat cgaaaatgat aaaaaacagc
    14701 ccaaactctg gactgcaacc gtttcgaaag atatcatcga atatgggaaa tgggattcgc
    14761 tgcaagtgaa tgctaatatc cccgaaaact ttggcgataa tgacgtaata agaatatatt
    14821 tctggtacac gggaaaaaat aaagtctgga tagatgacgt gaacattctg ttgcaaactg
    14881 agaatctgaa tgctgcatcc gatacaattt tattgattaa aaattaacta ctctaaatat
    14941 atttcctatc tttcgaaaat aaatccttat cattatttga gttaaaactt catcctatga
    15001 aaaagataaa acatcccata tcggcagcaa gtctgctttt gtgcgtgatc gtattaatta
    15061 gcgttcgatt gagccatcct ccaaagaata tattatcgta cgacactttc ggctattata
    15121 tttatttacc cgcccgccat atataccacg atccggggat acacaacttc gaatgggtaa
    15181 aagaaataaa ccaaaaatat aataatactc caacattcta tcagttttca gaaggaataa
    15241 atgggcagaa agtcatacgt tttaaccgtg gtatttccta ccttctggct cccggattct
    15301 atgtcggtca tgcctgggca aaattaagcg gagcccctca ggatggcttc tccaaaccct
    15361 atcaacaagc catttggatt tggggaatga tttttaatct cttaggattt tatctattaa
    15421 aaaagattct gcttcgctat ttcaacgatc taactacagg agtaacgctt attgtccttc
    15481 tcctgataag caatctttat ttcttctatg gttatggtag cgatattcca catgtctatt
    15541 tgttcactct caacgctgcc ctcatctggt ttactattca ttggcatcat catctgaaga
    15601 tatgggatgc cgcccttatt ggtcttaccc ttggaatgat cgcaatttca cgtattagcg
    15661 aggtactcat cgttttcatt ccattactgt ggggagttaa gaataaagaa actctcaaat
    15721 cgaaattcag cttgttcttg aataaatggc cacatgttct ggtagcaatc atcgctggaa
    15781 ttattcccct attgagccag atcatatact ggaaaagtgt gtccggagaa tatttgtatc
    15841 aaacttataa tgatcccggc tcaacactcg atttgatgaa tccacggttt tttcatactc
    15901 tacttagttt ccgcaaggga tggctcatct atgcgcctgt aatgatactt gcgttgtggg
    15961 ggatttacaa ggccttccgc aaaaaggaag aatgggcatg ggcaatactg gtgtatattg
    16021 cgctcgatat ttatgtaatt tccagcttta cgagcttgtt aagctatggc tggagggctt
    16081 ttcttcaatc gtatgctgca cttgtccttc caatgggagt atttatacaa tggatgcttt
    16141 ccaaaaaacc tctaaccata gttggttggt gcatgatcct tgtgatttta ggtgtgataa
    16201 atatatttca ggcatggcag atcaatatgg gtattattga cggttcgcgc atgacgatga
    16261 aatattacgt ttcggttttt atgaagaaac atcctcctga aaatgcaaag aaatatcttc
    16321 tggttcagcg ctctgccacc ggcagagaag atttgaaaga aaccgaaagc tatttcaatc
    16381 atgtcttgaa attttacgat ttcgaaacac ccaatcccaa acttcaatca catatcgatt
    16441 ccatcgtttc tttctcggga agatattgtc tgagactgga tagtaccatc gaatttaccc
    16501 ctggcctcga atgtacttat ggcgatataa gcaatcgcaa atgggtatgg ataaggctca
    16561 gtgcgagagt ctatcctgcc caaccgctcg aaggttcttc ggttttacta gtaacccacg
    16621 ccattcgaaa cggacaggct tataaatata cagcgagaaa aatcgcagat acccttttcc
    16681 atctcaaacc taatcagtgg aattatgttt ggcacgatta catgacaccc gaacctattt
    16741 cagccgaaga tgggattaaa agctatatct ggaaccgaaa taaaaatccc gtttttgtcg
    16801 acgacatccg catcgaagta ttcgaacctc tttcaagtat catcgaataa attctgagac
    16861 ctgctataga ttttattgta atggtgatcg aaaatatgga atttattttt cgataatttc
    16921 aaaacatgag gtgatcttcc taccccaagg ggactcgcat tatctttcaa ttttagtttt
    16981 cattctacga catccgccta cctccatttt ttcttcacac tttcagatat taaatttttt
    17041 gaaatttttg ttataaaata aaaaattcta atttcgtaaa cattcacttt tgaattcgag
    17101 taaaatggtt tcctgcacga atactttaaa tcgctgaaat atacttcatt tattatatcc
    17161 aattccattt tcctgttttc tgcattggga caccctaaaa atttttacaa acaattcggt
    17221 acagttcagc ttgctc
//
LOCUS       DAOORU010000030        17181 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4053_length_17181_cov_7.051384, whole genome shotgun sequence.
ACCESSION   DAOORU010000030 DAOORU010000000
VERSION     DAOORU010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17181
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4053_length_17181_cov_7.051384"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..375)
                     /locus_tag="PLF20_03405"
     CDS             complement(142..375)
                     /locus_tag="PLF20_03405"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80089.1"
                     /translation="MAYSSIILKKGKQEPLLRKHPWIFSGAIHHHEGEVNVGDIVAVY
                     SFDRQLMGYGLFEEGSLAVKMISFETSPDEEDF"
     gene            complement(403..654)
                     /locus_tag="PLF20_03410"
     CDS             complement(403..654)
                     /locus_tag="PLF20_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80090.1"
                     /translation="MLSFFDILLIIILGFFLLGYLMRWFLPMIMRWWTRHFFNQMGMQ
                     DAPSKKKKNKVEIKSAPRKIPNKSIMDEIGEYTDYEEIE"
     gene            complement(739..897)
                     /gene="rpmH"
                     /locus_tag="PLF20_03415"
     CDS             complement(739..897)
                     /gene="rpmH"
                     /locus_tag="PLF20_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOX80091.1"
                     /translation="MKRTFQPSRRKRVNKHGFRNRMSTANGRKVLSARRAKGRYKLTV
                     SDEKLDKR"
     gene            complement(1117..2577)
                     /locus_tag="PLF20_03420"
     CDS             complement(1117..2577)
                     /locus_tag="PLF20_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HOX80092.1"
                     /translation="MQQDIRILKPTALWKNFFSLTQVPRPSKNEERVRQFLIDFGKKL
                     GLETLTDEVGNVIIRKPATKGMENRKGIILQGHMDMVPQKNSNNPHDFNHDPIEAWVD
                     GEWVRARGTTLGADNGIGVAAAMAILESTDIPHGLIEVLITTDEETGMTGAQNLKPGL
                     LHGDILLNLDSEDEGELYVGCAGGVDGTFLFPYSKETLTSGMKAFKLSVTGLRGGHSG
                     LDINLGRGNANKILFRFLKHAQNHHQLRLACVEGGSLRNAIPREAFANVVIPETETEK
                     FLHCLDQYTEIIKNELESAEPNLSFTCENAPMPSHLIGQDTQWRLINAIYGCPNGVMR
                     MSNTFSGVVETSTNLANVKMNDNEVSVLCLLRSLVDSAKEDLSEMMASVFELAKANID
                     FSGSYPGWKPNPDSTILQLAKNIYKDKFGKEPEVKVIHAGLECGILGAAYPHWDMISF
                     GPTIRNPHSPDEKVNIPSVKRFWEFLLEILKTAPLK"
     gene            complement(2677..3294)
                     /gene="rsmG"
                     /locus_tag="PLF20_03425"
     CDS             complement(2677..3294)
                     /gene="rsmG"
                     /locus_tag="PLF20_03425"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOX80093.1"
                     /translation="MELISKYFPGLSEKQLNQFDMLKELYSSWNQRINVISRKDIEQL
                     EMHHILHSLSIARIIRFEPDTQILDAGTGGGFPGIPLAIYFPDVNFTLVDSIGKKIKV
                     VDEIILALGLENIETRKERIENLHEKYDFIVSRAFGQLSDLLRFSENLIRRDDINFLQ
                     NGILYLKGGKIEDELNRLGRPFHVYRLSNYFYEPWFETKKIVHIY"
     gene            3397..4467
                     /locus_tag="PLF20_03430"
     CDS             3397..4467
                     /locus_tag="PLF20_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HOX80094.1"
                     /translation="MNEIRNWAKAAVLVFIFSSGFYFSIEAQDTSNAGFKIREAYIQK
                     YKALAIREMVEYGIPASIKLAQAVLESGAGNGELAKIANNHFGIKCHKEWTGDKFFKD
                     DDSVKECFRKYESVEESYRDHSLFLRSRERYSNLFRFDICDYKAWAYGLKNAGYATNP
                     AYPQLLIKIIEEHQLTRYDHPGSDTLQLANIETDSSITRGGTADNNLTSERGPVDVSP
                     VKITKEGRKIYKNNDSKYIIYRQDDTPIRIAQEFDLYTWQIYDFNDLELDNPIKPGDM
                     VYIEKKKRKASEEFYIVKPGDDMHAISQKFGIRLQNLYELNRMNFGTEPQQGDTLWLN
                     KKKPADMQNRSPGILRIFKSKI"
     gene            4481..4762
                     /locus_tag="PLF20_03435"
     CDS             4481..4762
                     /locus_tag="PLF20_03435"
                     /inference="COORDINATES: protein motif:HMM:NF018563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOX80095.1"
                     /translation="MFEITEYYGGLVKCISFEVDGGQAVIGVMAPGEYEFTSSRTGYF
                     TVTTGVIFLMLPSHLVWKPYKPYETIIIPPDIKFKLKCTANTSYKLIYQ"
     gene            complement(4773..7559)
                     /locus_tag="PLF20_03440"
     CDS             complement(4773..7559)
                     /locus_tag="PLF20_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80096.1"
                     /translation="MNRSKTWLIVILLILGVPLLGGGIYWFLVKGPAREFKLIDAVPQ
                     NTSLIFITQDASQIWQKMKDKNEVWDALRMTETFSRLQDGIFAIDSLLKSQTNVNDFL
                     NDREVIISLHSVSRGKTEVLLLLPLSSNIEEAMLSDLLGNNAMDKVLNESMLHRYKRI
                     KATRLDKDGQVIYYTYRHRVLLASFSPEILAMSLQQLNKPHWISNNPQIENLLKTAGN
                     HVDGTLIFNFDYLPEIFKKWPASDFIPGFGKLKKFAHYASLDLYFQKKKIILNGYSLT
                     RPGDFLRLFRNQAPQEPTALNLLPEHTASYIYLCFDNFDNFIDSYNDYFASFSSPQQP
                     SQQLSFSAIQNLKESQIAEIASGLINTGSETALDNSLVILHSLNPQHFERMIKETLPS
                     TETGLSFTINQREFRKIDFNRFFGNFLSSILPDFQKAFFFRIGDYFLFSSKPQILHDY
                     IVQYLCGKTLVNTQGFKALSEISEERTNLWFYYNPPEAVAFHHFLFAAEAADNFDANL
                     PVLTLFDAVSVQVSGEGQFPYISTIIKHRIANEEILALYSSTILSSDTLADSHSRDIR
                     ADERLLWKNQLDHPAAQPVVIVNDLVKGKSFLLAFDQRQNTYLFDAAGSLKWKKQIEG
                     SGEAFCRVECIKINNQPVLFFITRSRLYAIDASGRMLAGYPIKLNIPLAGPASIFPQK
                     NSSELRIYYPGTDSLLYCINIKGKPINGWNPPKLTGQVVQAVKRFEFEGKTYFAVPYS
                     SGQVTMIQGNGKKAELFEQAFTNSSNSEFYLNETNRKGALLTTDQSGNLVYLSPKGPI
                     EKTVFDNFSKNHFFIYEDFDNNGSRDFIYLDGKQLVIYDRFKNILLQHQFREPVLQKP
                     LTLKIPGGKILLIINAGLKDGIFVFNDHGLISEKAFPGGNFFSGGILRNQKGINQPVL
                     VVTFNREIALWSIR"
     gene            complement(7715..8314)
                     /locus_tag="PLF20_03445"
     CDS             complement(7715..8314)
                     /locus_tag="PLF20_03445"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOX80097.1"
                     /translation="MLLRKYNLKLTEAGCDEAGRGSLVGPVFAAAVILPENFKAEGLN
                     DSKKLSAKKREYLREIIENTAICYAVATASREEIEKINVLNASTLAIHRALDQLKVRP
                     DMILVDGNRFKPYQQIEYACIVKGDGIYESIAAASILAKTYRDSFMNRLHEEFPQYGW
                     NHNKGYATREHLQAIQKFGLTPYHRRTFKISLQTRLSFD"
     gene            complement(8315..9298)
                     /locus_tag="PLF20_03450"
     CDS             complement(8315..9298)
                     /locus_tag="PLF20_03450"
                     /inference="COORDINATES: protein motif:HMM:NF040411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6427 family protein"
                     /protein_id="HOX80098.1"
                     /translation="MLRKILCTGQFRPFIVVAITTGLLWLNPLLNPPSSVSFDVFPLQ
                     KCLWGGLLNNRLGAGIIALILTIILAVGYYLILRAKKFVPYRSTITPFLFIVIISHNP
                     NLCTIHPLTLPLLCLFLSLLFALSFSPSEPNFQKSFWAGFFTGLASLFYPPAIFFLFY
                     LWIIVLLYSSSRGRDFLLVLLGFLSPLALIFEFMFLLDYPVLEFIQSVVVFYDLPVIS
                     ASISWLEWMFILIFTITILYIFFVYMSRLLQKPIQFRRTSWILAWFLFFSVISSVFSG
                     SVFIYHLTLVILPLTAFASAYFSEPLGTWRSDMTLCLLFLIALLQNLQIIK"
     gene            complement(9292..10641)
                     /locus_tag="PLF20_03455"
     CDS             complement(9292..10641)
                     /locus_tag="PLF20_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOX80099.1"
                     /translation="MGNSGDRLLIKIPKYSKRLQYTFQFLLKTLWGIEHEFTLNEDEF
                     NAWQGPKMIYGGSFQENEVWFDAVSLLFERNIAGFDTGKYSPYTGLFPVYGSDSALPF
                     DAFASTFYMLSRYEEYLPNRKDTHGRFPATESIAYREGFLDKAVVNRWAEMIVAILLK
                     RFPDLHFHKTSYQYVPTIDVDLAYSYLHKGFLRTLGGYVKSLIHADFEDVRSRTHVLM
                     GKERDPFDSFDALHHLHSSYYLKPVFFFLFSDYGQYDKNLSIYNPAFRNLIKKVADYA
                     TVGIHPSYACYENPQKLKKEIEDLSQCLHRQITHSRQHFLRLNFPETYRNLIHCGITD
                     DYSMGFADQPGFRAGTCNSFPFYDLDLDTATSLLIHPFAFMDGTLKDYLKIPKDQVVD
                     FVKPYIEEVKRYSGTLITLWHNETHGGSGRWEGWPEIYETIVKMGASSSIETSTKPC"
     gene            10783..12576
                     /locus_tag="PLF20_03460"
     CDS             10783..12576
                     /locus_tag="PLF20_03460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF011531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOX80100.1"
                     /translation="MDNFIVSARKYRPSTFDEVVGQQSIVQTLKNAIRTRQIAQAFLF
                     TGPRGVGKTTTARIFAKTINCQNLTPNFEPCNHCDSCKDFNNNTSLNIFELDAASNNS
                     VDNIRSLIDQVRIPPHHGKYKVYIIDEVHMLSQNAFNAFLKTLEEPPDYVKFILATTE
                     KHKVMATILSRCQVYDFKRITTEDIVHQLQHVAQNEGISYEVEALHVIAEKADGAMRD
                     ALSIFDQLVTYSGCHLTYQKVAESLYVLSYEDYFKMSEDLLGGKISDALILLNDIIER
                     GFEGQMFINGLGKHFRNLWLAKDPASVGLLEVSETTRNFYLTQAQKCSLQFLAKALNL
                     INQCDANYRMSLNKRLAIEITLINICQIENPFTNSKPKAPTTPSLTTEPNAQYSPKSS
                     PAATAAPSSSLSQKSSSLSPQVPKTSASPSQLPKSSPNNGNDSPKNGLSHFGLQTANL
                     RKISNPVEEKKSEKTEENQKHEQQIHDFTEEELLIKWTSFLDALNEKEGALVCSLRMA
                     KPRKMGNNTIVFRVFNSVIENDILSNQDRILDFIRSELSNSQIKLITEITASSENNIK
                     AYTPLERLKELTQRNPAVNEFMQKFNLQPEL"
     gene            complement(13020..13469)
                     /locus_tag="PLF20_03465"
     CDS             complement(13020..13469)
                     /locus_tag="PLF20_03465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80101.1"
                     /translation="MESEHCYQFALYDAGGNGLCCANGLGFFTLFDDKNVTIAEGTTF
                     GSEVLAQFYSQSGIGIEDLSKQYLSIIPNPVSHLSMIYFNMNTDGKVNLNIYNLNGSL
                     IFQKVSETLNKGEQKMKLNVERMNSGIYLIEIIMPDKKVLRQRFVVQ"
     gene            complement(13916..14719)
                     /locus_tag="PLF20_03470"
     CDS             complement(13916..14719)
                     /locus_tag="PLF20_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOX80102.1"
                     /translation="MKNLRILSLSLLLLLVLPLIAQQPKAQRLVLLEEFTSSTCGPCA
                     SVNPTIVQRLQQNPDKFTAIFYHVSWPSPGNDPMYLANTQENNARVNYYGVNSVPYSV
                     IDGNYYTGHPNGWTMTTINNRYAMPSPAEIQLQHYLNAAQDSIFVNMLVILTDMMTGS
                     QLVAQNVIIEKHIHFNTAPGTNGEKDFYNVMKKCFLEPVELVCPLLFRLVIMLLCNIH
                     GNWPMFMITTNLPPLASFRIIPRKRFFKHPTALLHLLLRFIAMMERFSP"
     gene            complement(14979..16077)
                     /gene="prfB"
                     /locus_tag="PLF20_03475"
     CDS             complement(join(14979..16016,16018..16077))
                     /gene="prfB"
                     /locus_tag="PLF20_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157754648.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOX80103.1"
                     /translation="MMNEDLKVLESRIEALRRFLDIDKKLKDVKEEEDITHANDFWND
                     PKSAEKILKSIQAKKVWIERFEKVKNLYEDLRVLVDFWDMGEATEEEVNRHYQETLQE
                     VEELEFMNMLSAEEDRMGAIMEINAGAGGTESQDWASMLMRMYIRYGERQNFKVTELD
                     LHEGDVAGIKNVVLEFDGEYAFGYLKAENGVHRLVRISPFDSNARRHTSFASVYIYPI
                     VDENIEIDINPADITWETYRSSGPGGQNVNKVETAVRLKHEPTSIVIECQVTRSQQQN
                     KEKALQMLKSKLYEIELRKKREKIDAIEAAKKKIEWGSQIRSYILHPYKMVKDHRTNY
                     ETGDTQAVLDGDIQDFIKAYLMQNGNIARKQ"
     gene            complement(16098..16589)
                     /locus_tag="PLF20_03480"
     CDS             complement(16098..16589)
                     /locus_tag="PLF20_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOX80104.1"
                     /translation="MKNPSSKIHSSDEGLFEKAKRWCAFEERCSYDLRLKLEQWGVTE
                     DKISEIIQQLTREGFIDDGRFARMFVQGKFHNLKWGKIKIKAELQRHHISNELTEQAI
                     ALLDDDEYKKSLLKILNNKRKSLSKELPDVARQKLIRFALSKGYEAELIYSLLGKTNL
                     IEE"
BASE COUNT         5124 a         3368 c         3364 g         5325 t
ORIGIN      
        1 aaaacttaat cttcatctcc tctgaaaaag ttgcatttat tagttgaatt tagtattaat
       61 aaaacggtaa gcattggtag ttggattatc gatcaaaccc aaagcttttc ttaaagagaa
      121 ggctgtttcg aatttttttt gctagaaatc ttcttcatcg ggcgatgttt caaaagagat
      181 catttttacg gcaagcgaac cctcttcgaa tagtccatat cccataagct gccggtcgaa
      241 tgaataaaca gcaacaatat cacccacatt tacttcaccc tcgtgatggt gaatggcacc
      301 cgaaaaaatc catggatgtt ttcgcaataa aggctcttgt tttccttttt tcaaaatgat
      361 cgacgaatag gccatttccc cagttttctt attggatagc ttttactcta tctcttcata
      421 atctgtatat tctcctattt catccatgat agatttatta gggatctttc ggggagctga
      481 ctttatttcc actttatttt tcttcttttt tgaaggagca tcttgcatgc ccatttggtt
      541 aaaaaaatgc cttgtccacc agcgcattat cattgggagg aaccaacgca tgagataccc
      601 cagtagaaag aatcccaaaa tgatgatgag caggatatcg aaaaaactaa gcattttata
      661 gggtaatagg atcaaaaaca aataaggagg caggattttc cccctccttc tcttagctaa
      721 atatttgcta cgaagaagtt atctcttatc gagtttttcg tcagaaactg taagtttata
      781 gcggccttta gctctacgag ccgagagaac tttccgccca ttagctgtgg acattctgtt
      841 tctgaacccg tgtttgttta ctctttttct tcttgaaggc tgaaaagtcc ttttcatttc
      901 attaaatttt tatttcattg atgttcaagg gaacaactat caattacact gttttagaaa
      961 tggggtaact ttatataatt ttaaaaaaat cgaagtgcaa aggtacaaag atttattcaa
     1021 taaaaaagca aagggatata agataaaaaa taatgctcca tataaaaaaa gcccctgtac
     1081 aggggctgat taagtaaata tttaacttat aaatttctac ttaagggggg cagttttaag
     1141 aatttcgagc agaaattccc agaagcgctt cacgctgggg atattcactt tttcgtcggg
     1201 gctatgaggg ttacgaatag taggcccaaa cgagatcatg tcccaatggg gataagctgc
     1261 gcctaatatt ccacattcga ggccggcatg aatgaccttt acctcgggtt ctttcccaaa
     1321 tttgtccttg taaatgttct tcgccagttg caatattgta ctatcaggat taggcttcca
     1381 ccctggatac gaacccgaga agtcgatatt tgccttggct agttcaaaaa ccgaagccat
     1441 catttctgaa agatcttctt ttgcagaatc gactaagctg cgtaacagac atagaacaga
     1501 aacttcatta tcgttcattt tcacatttgc taaattagtc gaggtttcga ctacaccgga
     1561 gaatgtattg ctcatcctca tcactccatt gggacagcca taaatggcat tgatcagtcg
     1621 ccactgagta tcttgaccta tcaaatgtga aggcattggg gcattttcac atgtaaagct
     1681 cagattaggt tcagctgatt cgagttcatt tttgataatt tctgtgtatt gatcgaggca
     1741 atgaaggaat ttttcggttt ctgtttctgg aataaccaca ttagcaaatg cttcgcgagg
     1801 aattgcgtta cgtaagcttc ctccctcaac gcatgctaag cgcagttgat gatggttctg
     1861 agcgtgttta aggaaacgga agagaatttt attggcattt cctcttccta aattgatgtc
     1921 aaggccagag tgcccacctc ggagcccggt aaccgaaagt ttgaatgctt tcatgcctga
     1981 ggtgagagtt tctttcgaat aggggaaaag gaatgtgcca tccactcctc ccgcacaacc
     2041 aacataaagt tcgccctcat cttccgaatc gagattcagc aagatgtcgc cgtgcaataa
     2101 tcctggtttt agattctgag cgcccgtcat tcctgtttct tcatcggttg taattagtac
     2161 ctcaatgagg ccatgcggta tgtcggtgga ttcgagaatc gccatagctg cggctactcc
     2221 aataccgttg tcggcaccca gagtagtccc tctagctctc acccattcgc catcaaccca
     2281 tgcttcgatg gggtcgtggt tgaaatcatg aggattgttg ctgttctttt gcggaaccat
     2341 gtccatatgg ccttgcagaa ttatgccttt gcgattttcc atgccttttg ttgcaggctt
     2401 gcggataata acgttaccaa cctcgtcggt cagggtttct aaacctagtt ttttgccgaa
     2461 atcaatcaag aattgtctga cgcgttcttc attcttcgaa ggccgtggaa cctgtgtcaa
     2521 gctgaaaaaa ttcttccaga gggcagttgg ttttaaaatt ctaatatctt gctgcataaa
     2581 tagtttggtt taattacaat aaattgaact tgcgtagctg caaaattaga gaaatattat
     2641 ccttgaaaga aaaagaatat ggatatggag aacaagttaa taaatatgaa caattttttt
     2701 tgtctcgaac caaggctcat aaaaataatt ggacagacga taaacgtgaa aaggtcttcc
     2761 caatcgattc agctcatctt ctattttccc tcccttcaga tagagaatgc cattttgaag
     2821 aaaattgata tcatcgcggc gaatgaggtt ttctgagaac ctcaataagt cggaaagttg
     2881 tccgaaagct ctgcttacaa taaagtcata tttctcgtgc agattctcga tgcgttcctt
     2941 gcgagtctct atgttttcga gtcccaaggc aagtatgatc tcatccacca cctttatctt
     3001 ttttccgatg gaatctacca gagtaaagtt aacgtcggga aaatatatcg ccaaaggaat
     3061 tccggggaat cctcctcctg tgccagcatc aagaatttga gtatcgggct caaaacgtat
     3121 gattcgggca atacttaagg aatgaagaat atgatgcatc tcaagttgct caatgtcctt
     3181 gcgtgaaata acgttgatac gctgattcca ggatgaatag agttctttga gcatatcgaa
     3241 ttgattcagt tgcttttcgc ttagccctgg gaaatatttc gaaatgagct ccattaaatg
     3301 ctcgattgag ttgatgggtg caaaactatg caaatcgggg gttgcggcca aatgctttca
     3361 tggttgattc tttcgggcaa ttattaattt tgccgtatga atgaaattag aaattgggcg
     3421 aaagccgctg tgttggtttt tattttttct tccggcttct atttctctat cgaggcacaa
     3481 gatacaagta atgcaggatt taaaatacga gaggcctata tccagaaata taaggctctg
     3541 gccatacgcg agatggtaga atatggcatt ccggcaagca taaaattggc tcaggctgtt
     3601 cttgaatcag gcgcaggtaa tggagaatta gccaaaatag cgaacaatca cttcggtata
     3661 aaatgccata aagaatggac tggtgataaa tttttcaagg acgatgacag tgtaaaggaa
     3721 tgtttcagga aatatgagtc ggtagaggaa tcctatcgcg atcattcttt gtttttgcgt
     3781 tcacgtgagc gctattccaa tctgtttaga ttcgatattt gcgattacaa ggcctgggct
     3841 tatgggctga aaaatgccgg gtatgccacc aatccagcct atcctcaact cttgatcaaa
     3901 attattgagg aacaccagct cacacgttac gaccatcctg gttccgatac cttgcaactg
     3961 gcaaatattg aaaccgattc atcaataacc agaggtggga cggcagataa taaccttact
     4021 tcagagcgag gtcccgtgga tgtttcaccg gtaaaaatta ccaaagaagg tcgaaagata
     4081 tacaagaata acgacagtaa gtatattatt tatcggcaag atgatacacc cataagaata
     4141 gctcaggaat tcgacctcta tacatggcag atatacgatt ttaatgacct cgaactggat
     4201 aacccgataa agcccggaga tatggtgtac atcgaaaaga aaaaacgaaa ggccagtgag
     4261 gagttttata tcgtgaaacc tggtgatgac atgcacgcca tttcgcaaaa atttggcatc
     4321 cgtctgcaaa atctctatga actgaaccga atgaactttg gtaccgaacc tcagcaagga
     4381 gatactttat ggttgaacaa aaaaaagcct gcagatatgc aaaatcgttc tccgggaatt
     4441 ttacgtattt ttaaatccaa aatttaatga ttgttattag atgtttgaaa taacagaata
     4501 ttatggtgga ttagtgaagt gcatctcttt tgaagtggac ggcggccaag ccgttattgg
     4561 tgtgatggca cccggtgaat acgaatttac ttcctcccgc acgggatatt ttaccgtaac
     4621 cacaggagta atatttctca tgctgcccag ccatctagtg tggaaacctt ataaacctta
     4681 cgaaaccatc attatccctc ctgatatcaa attcaaattg aagtgcactg ccaatacttc
     4741 atataagttg atttaccaat gatgtcgtgt tattatctaa tcgaccaaag agctatctca
     4801 cgattgaagg taactacgag aaccggctga ttgattcctt tctgattcct taatatccct
     4861 ccactaaaaa aattccctcc ggggaaagct ttttctgaaa tgagcccgtg gtcgttaaaa
     4921 acaaatattc catctttcaa acctgcattt atgataagga gtattttgcc gcctggaatc
     4981 ttcaatgtca gtggcttttg caacacaggt tctctgaatt gatgctgaag caatatattc
     5041 ttgaaccggt cataaataac cagttgtttc ccatcgagat agataaaatc gcgactgcca
     5101 ttattatcga aatcctcata aataaaaaaa tgatttttcg aaaaattgtc gaatactgtt
     5161 ttttcgatag ggccttttgg gctcaaatag accagatttc ccgactgatc ggtagtgagc
     5221 aaggcgcctt tccgattggt ctcgttcaaa taaaattcag agttggaaga gttggtaaaa
     5281 gcctgttcga aaagttccgc ctttttgcca ttgccttgaa tcatcgtgac ttgccccgat
     5341 gagtaaggaa cagcaaaata ggtctttcct tcgaactcga aacgtttcac agcctgaaca
     5401 acttgtccgg ttagttttgg gggattccat ccgttgatag gtttcccttt tatgttgatg
     5461 cagtacaata aactgtcggt tccgggataa taaatacgaa gctctgatga atttttctgg
     5521 ggaaaaatag atgccgggcc tgccagagga atgtttaatt tgataggata tcctgcaagc
     5581 atcctaccgg aagcgtcgat ggcataaagc cggctgcggg taataaagaa cagtaccggt
     5641 tgattattga ttttgataca ttctactcta caaaatgctt ctcccgatcc ttcgatttgt
     5701 tttttccatt tcaaagagcc cgctgcatcg aataaatagg tattttgtct ttggtcgaat
     5761 gccagaagga agcttttgcc tttgaccaaa tcattgacaa tgactactgg ctgtgcggct
     5821 ggatggtcaa gctggttttt ccataataag cgttcgtctg ctcgaatatc tctcgagtgc
     5881 gaatctgcga gagtatcact cgataggatg gtggaagaat aaagtgccag aatttcttcg
     5941 tttgctatgc gatgtttgat aatggttgag atgtagggga attgtccttc acccgagact
     6001 tgaaccgaaa ctgcatcgaa aagagtgagt acgggtaagt tagcgtcgaa attgtctgct
     6061 gcttctgctg caaataagaa atgatgaaaa gccacagctt cagggggatt ataatagaac
     6121 cacagattgg ttctttcttc cgatatttct gagagagcct taaatccttg cgtgttcacc
     6181 agagttttcc cgcacagata ttgaacgatg taatcatgaa gaatttgagg tttagaacta
     6241 aaaaggaaat aatccccgat tctaaaaaaa aatgcttttt gaaaatcagg caaaatactc
     6301 gataaaaaat tgccaaagaa gcgattgaag tctatttttc ggaattctct ctggttaata
     6361 gtaaatgata gtccagtttc ggtcgatggc aaggtttctt ttatcattct ttcaaaatgc
     6421 tgaggattga gtgaatgaag aatgactagt gaattatcca gagccgtttc gcttcccgta
     6481 ttgatcagtc cagaagcaat ttcggctatt tggctttctt ttagattttg tatggctgaa
     6541 aagcttagtt gttgagaagg ttgttggggg cttgagaaag aagcaaagta atcattatat
     6601 gaatctataa aattgtcgaa attgtcgaaa cacaggtaaa tatagcttgc tgtatgttcg
     6661 ggaagaagat taagtgctgt aggttcttga ggtgcctggt ttctgaacag tcgtaaaaaa
     6721 tctccggggc gtgtaagtga ataaccattc agaatgatct tttttttctg gaaataaaga
     6781 tcgagtgaag cataatgggc aaatttcttg agtttgccaa atcccggaat aaaatcgctt
     6841 gccggccatt tcttgaaaat ctcgggcagg taatcgaaat tgaagatgag tgtaccgtcc
     6901 acatggtttc ccgcagtttt caaaaggttc tctatctgag ggttgttgga aatccaatgg
     6961 ggcttattga gttgttgcaa gctcatagct agaatttcgg gcgaaaaaga agctaataaa
     7021 accctatgcc tgtaagtata ataaatcacc tgtccatctt tatccaatct agtggctttg
     7081 attcttttat atctatgaag catagattcg tttaaaacct tatccatagc attgtttccc
     7141 agaagatcgg ataacattgc ttcttcgatg ttcgaagaca aaggtaatag gagaagaact
     7201 tcggttttcc ccctcgaaac ggaatgtaaa gatatgatga cttccctgtc attgagaaaa
     7261 tcgttgacat tggtttgaga cttgagaagc gagtcgattg cgaatattcc atcttgaagt
     7321 ctcgaaaagg tttctgtcat tcggagagca tcccaaacct cattcttatc tttcatcttt
     7381 tgccaaatct gagaggcgtc ttgggtaata aaaataaggc tggtattttg aggtacagca
     7441 tcaataagtt tgaattcacg agccggacct ttcactaaaa accaataaat gcctcccccc
     7501 aataaaggaa ctcccagaat cagcaagata acgattaacc atgttttcga tctgttcatt
     7561 tttttacttc agaatgtttg cgctgctaaa cccttagagt tttcataata taatccagtt
     7621 tctctctatt cgagagtatt ataattttaa ttgcaaaatt acatttaaac ctgaagccat
     7681 ttgtattttt cgaatttgct ctgaagagcg gctcttagtc gaacgataaa cgtgtttgga
     7741 ggctgatttt gaaagttcgc cggtgatagg gagtaagtcc gaatttctga atggcctgta
     7801 agtgttccct tgtggcatag cctttattat gattccaacc atactgagga aactcttcat
     7861 gtaagcgatt cataaaggaa tcgcgatagg ttttggcaag gatagaagct gcggctatgc
     7921 tttcgtaaat cccgtcacct ttcactatac aggcatattc gatttgctgg taaggtttaa
     7981 accggttacc gtctacaaga atcatatcgg gtcgcacttt gagctgatcg agtgccctgt
     8041 gtatagccaa agtgctggca ttcaatacgt ttattttttc gatttcttct ctcgaagcag
     8101 tagcaacggc ataacaaatg gctgtgtttt cgataatttc ccttagatat tctctttttt
     8161 ttgcgctgag ttttttcgaa tcgttcaagc cttcagcctt aaaattttcg ggcaaaataa
     8221 cggcagcagc aaataccggc cccacaaggc ttcctcgtcc ggcttcatcg cagccggctt
     8281 ccgtcaactt caggttatac tttcgcagca acatttattt tataatttgg agattttgca
     8341 ataaagctat taaaaatagt aaacacagag tcatatcact tcgccatgtc cccaaaggtt
     8401 cactaaagta agccgaggca aacgccgtca aaggaagaat taccagagtt aaatggtaaa
     8461 tgaataccga acccgaaaaa acagaagaaa tcaccgaaaa gaataagaac cacgccagta
     8521 tccagctcgt acgccggaat tgtatcggct tttgcaataa gcggctcatg taaacgaaaa
     8581 aaatatatag tatggtgatg gtaaaaatga gaataaacat ccactctaac catgaaatgc
     8641 tggctgagat aacgggtaaa tcataaaata ctaccacaga ctgaatgaat tccaatacag
     8701 gataatcgag cagaaacatg aactcaaaaa ttaaagccag aggactgaga aaacccaata
     8761 aaactaataa aaaatctctt cctctcgacg aactgtataa aagtacgata atccataaat
     8821 agaaaagaaa gaatatggcc ggaggataga acagggaggc taatcctgta aaaaaaccag
     8881 cccaaaacga tttttgaaaa ttcggttcag agggagaaaa actgagtgca aaaagtaggc
     8941 tgagaaataa acagagtaaa ggtaaggtga gcggatgaat agtgcaaagg tttggattat
     9001 gactaataat tacgataaag agaaacggag ttattgtgct tcggtaagga acaaattttt
     9061 ttgccctgag gataagataa tatcccactg caaggataat cgttagaatc agagctataa
     9121 ttcctgctcc caaccggttg ttcaataggc ctccccataa acatttttgc agagggaata
     9181 catcgaaaga aactgatgat ggtggattca ataaagggtt caaccacagt aatcctgtcg
     9241 ttatggccac aacaataaat ggccggaatt gacccgtaca taaaattttc ctcagcatgg
     9301 ttttgtggat gtttcaatgg aagatgaagc tcccattttc acaatggttt cgtatatttc
     9361 gggccagcct tcccatcgtc cactgccccc atgagtttca ttgtgccata gagtaatcaa
     9421 tgtacctgaa taacgcttta cttcttctat atatggctta acaaaatcaa ctacctggtc
     9481 ttttggaatc ttcaaataat cttttagagt tccatccata aaggcaaaag gatgaatcaa
     9541 caaagatgtg gccgtatcca gatcgaggtc gtagaaagga aaggaattgc aggtaccggc
     9601 tctgaatcca ggttgatcag caaaacccat tgaataatca tcggtaattc cgcagtggat
     9661 gaggttgcga taggtttcag gaaaattcaa tctcaagaaa tgctgacgac tatgtgtaat
     9721 ttgcctatgc aagcattggg ataggtcttc gatttctttt tttaatttct gaggattttc
     9781 atagcaagca tacgaaggat gaattcctac cgtagcataa tcggccacct tcttgatcaa
     9841 gtttctgaaa gctgggttat aaatcgacaa attcttgtcg tattgtccat aatcactaaa
     9901 caaaaagaaa aatactggtt ttaagtaata ggaggaatgc aagtgatgca aggcatcaaa
     9961 actatcgaaa ggatcacgtt ctttgcccat taatacatgc gttcggcttc ttacgtcttc
    10021 aaaatctgca tgtatcaaac tttttacata tcctccgaga gttcttagaa atcctttgtg
    10081 caagtaacta taagctaaat caacatctat agtgggaaca tattggtagg aagttttatg
    10141 gaaatgcaaa tcaggaaatc tttttaataa gatagctact atcatttctg cccaacgatt
    10201 cacaaccgct ttgtcgagaa atccttcacg ataggcaata ctttctgtag ctggaaaacg
    10261 tccatgtgta tcttttcgat tgggaaggta ttcttcataa cggctcaaca tgtaaaaggt
    10321 cgaggcaaat gcatcgaaag gcaaagccga gtctgaacca taaacgggga aaagaccggt
    10381 atagggcgaa tattttcccg tgtcgaaccc tgcaatgttt cgctcgaata ataaggaaac
    10441 cgcatcgaac caaacttcat tttcctgaaa acttccccca taaatcatct tcggaccttg
    10501 ccatgcattg aattcatcct cattgagcgt aaattcatgc tcgatccccc ataacgtctt
    10561 caacagaaac tgaaatgtat attgaagtcg cttgctgtat tttgggattt ttatcagtaa
    10621 tctgtcgcca gaattgccca taaatattct aaaataaaat gttttcgatt aaaaacttgc
    10681 gaaagttaca ctgaatgaaa accacaaagg tacaaaggcc caacatatat tcatttggta
    10741 aaaaagcatt aagtttgacg ccagattctt tttatttaca gcatggataa ttttattgtt
    10801 tcagcaagga aatacaggcc aagcactttc gacgaagtag tggggcaaca aagtatagta
    10861 caaaccttga aaaatgctat acgtactcgg caaattgctc aggctttttt atttacaggc
    10921 ccgcgtggcg taggtaaaac aaccactgca cgaatctttg ccaaaaccat caactgccag
    10981 aatctcaccc ccaacttcga accatgcaat cattgtgatt cgtgtaaaga tttcaataat
    11041 aatacctcat tgaatatttt cgaattggat gccgcttcca acaattcggt agataatatc
    11101 cgttcactga tcgatcaggt gcgtattccc ccacatcatg gaaaatataa ggtgtacatc
    11161 atcgatgaag tacacatgct gtcgcagaat gccttcaatg cattcttaaa gacacttgaa
    11221 gaacctcccg attatgtaaa gtttattcta gctacaaccg aaaaacacaa ggtaatggcc
    11281 actatacttt cacgttgcca ggtttatgat ttcaagcgta ttactactga ggatatcgtt
    11341 caccagctcc aacatgttgc tcaaaatgaa ggcataagtt atgaagtgga ggccttgcat
    11401 gtgattgccg agaaggccga tggagctatg cgcgatgctc tttccatctt cgatcagctg
    11461 gttacctatt cgggctgtca tctcacctac cagaaagtgg cagaaagcct ttatgtgctt
    11521 agttatgagg attatttcaa aatgagtgag gatttgttag gcggaaaaat cagcgatgcc
    11581 cttattttat tgaatgatat catcgaacgt ggcttcgaag gccaaatgtt tatcaatggg
    11641 ttgggcaaac attttcgtaa tctttggttg gctaaagacc ccgcctcggt tggattgctt
    11701 gaggtttcgg aaacgacaag aaatttttat ctgacacagg cccaaaaatg ttctcttcaa
    11761 tttctggcca aggcactcaa tctcatcaac caatgcgatg ctaactaccg catgagtctc
    11821 aataaaagac tcgccatcga aattactctt attaatattt gccagatcga aaatcctttt
    11881 accaactcga aacccaaggc gccaaccacc ccttccctta ctaccgaacc taatgctcaa
    11941 tattccccta aatcttcacc cgctgccact gctgcccctt cttcttctct ttctcaaaaa
    12001 tcttcctccc tatctcccca agtgccaaaa acctcagcct ctccctccca acttcccaaa
    12061 tcttccccaa acaacggaaa tgattctcca aagaacgggc tctcccattt tgggctacaa
    12121 accgctaact tgagaaaaat ttcaaatccc gtcgaagaaa aaaaatcgga aaaaactgaa
    12181 gaaaatcaga aacatgaaca acaaattcat gacttcacag aagaagaact cttaataaaa
    12241 tggacatcct ttctcgatgc tttgaatgaa aaagaaggag cattggtttg ttcgcttaga
    12301 atggccaaac cgcgcaagat gggaaataat actattgtat ttcgggtgtt taatagtgta
    12361 atagaaaatg atattctcag taaccaagac cgaatcttag atttcattcg atcggaactc
    12421 tctaattctc aaataaaact catcaccgaa ataaccgctt catccgagaa taatattaaa
    12481 gcatacactc ctttggaaag attaaaggaa cttacgcaac gaaatccagc cgtcaacgaa
    12541 ttcatgcaaa aattcaacct tcagcctgaa ctgtaacttt catcgaaatc gatttttttt
    12601 cgaagtttta attaatttgt ttgatgcaag ttatcgggaa acagccggac ttatttctta
    12661 tggccctgtt ctgtcttttt tttatcgttt tttggggaat tcatagggta tttccttaaa
    12721 gtaagattta ttgcatgtat ttttgaaaat cgaaacatat aataacactt ttcagtggcg
    12781 gcaagggttc cagagagttc gaactgatga tataaacgta agatattggt taataataat
    12841 ataaaattaa ttatgattat tttcttttag tattaaaccc tggcatatta attgtttcca
    12901 actaaatggt tttggttaac atttaatgga ttttgtttag taataaagga caggctactt
    12961 ttgagggcag cctgttttta ttataagagt catgaaaaaa atgagatgag tattaggact
    13021 tattgcacga caaaacgctg ccgcaacacc tttttatcgg gcattataat ttctatcaaa
    13081 taaataccag aattcattcg ttcaacattt aacttcatct tttgctcgcc tttgttcaat
    13141 gtttctgaaa ccttttgaaa gatgaggctt ccgttcaagt tgtagatatt gagatttact
    13201 tttccatctg tattcatatt aaagtagatc atggataaat gactcaccgg attaggaatg
    13261 atagatagat attgtttcga taaatcttcg attcctattc cgctttgcga atagaactga
    13321 gcgagtactt cactcccaaa ggtagtacct tcggcaatgg ttacgttttt atcgtcaaaa
    13381 agagtaaaga agcccaatcc gttggcacag cacaaaccat ttcctccagc gtcgtaaaga
    13441 gcaaattgat agcaatgttc ggattccact ttaatagttt ctttgatcag ggtgctggct
    13501 tgtgcataag gcccgcctga gcttaccaga ctgccatcgc ttgtcttgat gctccatgtg
    13561 atttcttcgg gtttattgtc ggttttaatc cataagttca gaaccgttgt tgcagtttta
    13621 ggttctgaaa gatggaaggt aagggtgtca ttcttacgat attcatcctg taattgattg
    13681 accttatcga tataaatttt aagagtattc gaattttggg gtgcaaacaa atattcaggc
    13741 aaagcaatat ttttcgattg tagaaatcca atgttgcccg tccatgtata ttcctgttca
    13801 ggctggttgt ctattctata ttttaatgta atagaactga gggaattgga accattattt
    13861 cgaacgcgaa taacaggagc aacctttccc gtacaatttt cgggcgctac attgcttaag
    13921 gagagaatct ctccatcatt gctataaagc ggagtaagag gtgcaggaga gctgttggat
    13981 gtttgaagaa cctctttcga ggaattattc tgaatgaagc caatggcggc aagttcgttg
    14041 ttatcataaa cattggccag tttccatgaa tattgcataa taacataatc accaggcgaa
    14101 agaggagtgg gcaaactagt tccaccggct ccaggaagca ttttttcatc acgttgtaga
    14161 aatctttttc gccattagtt cccggagctg tattaaaatg aatatgtttt tcgatgatta
    14221 cattttgtgc aaccaattga ctgcctgtca tcatatctgt aagaatcacc agcatattca
    14281 cgaagatact gtcctgggca gcattaagat agtgttgaag ctgaatttcg gcaggtgagg
    14341 gcatggcata acgattattg atggtagtca tagtccatcc attgggatgc cccgtataat
    14401 aatttccatc gatcaccgaa taagggactg aatttacccc ataatagttt actcttgcat
    14461 tattttcctg ggtattggcc agatacatag gatcgttccc cggtgaaggc cagcttacat
    14521 ggtaaaatat ggcggtaaac ttgtcggggt tctgttgcag ccgctgaaca atagttggat
    14581 tcaccgaagc acatggtccg caggttgatg aggtaaactc ttcaagcaat acgagccgtt
    14641 gagctttggg ctgctgtgct atcagcggaa gcactaaaag cagcaataga ctgagactga
    14701 ggattcgtaa atttttcatg ttttggtgtt tggtctttga atttgataaa taatagttta
    14761 ttcgattttt tgaattttat ttaaaaatta ttctttattt attattttaa aataattttt
    14821 ttaaatattt catcttattg ttaggtgata aaagttccgg taaaaatagg tttttttaat
    14881 gacaattggc atttattatt ccgattttat ggttaaagga tgattcggac attttaaaaa
    14941 aggggtgtaa agctgatgca tacttcgaat tttgtatatt attgttttcg agcaatgttg
    15001 ccattttgca tcaggtaggc cttgatgaag tcttgaatat caccatcgag aactgcttgt
    15061 gtgtctccgg tttcataatt ggttcggtga tctttcacca ttttataagg atgcaggata
    15121 tagctacgta tttgtgatcc ccattcaatt tttttctttg cagcttcaat ggcatctatt
    15181 ttttctcttt tcttgcgcag ttctatttca tagagtttcg atttcagcat ttgcagggct
    15241 ttttccttgt tttgctgctg tgagcgtgta acctgacatt cgatgacaat gctcgtaggt
    15301 tcatgtttaa gcctgacagc ggtttctacc ttgttaacat tttgtccgcc cgggccgcta
    15361 ctgcggtagg tttcccatgt aatatcggcc ggattgatat cgatttctat attttcatca
    15421 acgataggat atatataaac tgatgcaaaa gaagtatgac gtcgtgcgtt cgaatcgaaa
    15481 ggtgagatgc gcacaagacg atgcacccca ttttcggcct tcaaatatcc aaaagcatat
    15541 tcaccatcaa attcaagaac tacatttttt attcctgcta catcgccttc atgtaaatcg
    15601 agttcggtca ccttgaagtt ctgcctctcg ccatatcgaa tgtacatgcg cataagcatg
    15661 gaagcccagt cctgactctc cgttcctcct gccccagcat tgatttccat gatggcaccc
    15721 atacggtctt cctcggccga aagcatattc ataaattcaa gttcttctac ttcctgaagg
    15781 gtttcttgat aatgcctatt cacctcttcc tcggtggctt cgcccatatc ccaaaaatca
    15841 accaaaaccc gtaaatcttc atataaattc tttactttct caaaacgctc tatccatact
    15901 ttttttgcct gaatggactt aagaattttt tctgcagatt tgggatcatt ccaaaagtcg
    15961 tttgcatggg ttatgtcttc ctcttccttt acatctttga gttttttatc tatgtcaaag
    16021 aaacctcctc aaggcttcta tccttgattc gagaacttta aggtcttcat tcatcatatt
    16081 ttgagtcatt taaaaaatta ttcttctatg aggtttgttt tacccagtaa gctgtaaatc
    16141 agttcagctt cgtatccttt tgacagggca aaacgtatga gtttctgacg ggcaacatct
    16201 ggcagttctt ttgatagcga ttttcgtttg ttgttcaaga ttttcaataa gcttttttta
    16261 tattcatcgt catccagcaa ggcaatggct tgctctgtca attcattcga tatgtggtgc
    16321 cgttgaagct cggccttgat ttttatcttg ccccatttta aattgtgaaa ttttccttgg
    16381 acaaacatgc gggcaaaacg tccatcatct ataaaccctt cacgggtaag ctgctgaata
    16441 atttctgaga ttttgtcttc agttactccc cattgctcga gtttaagcct gagatcgtag
    16501 ctgcatcttt cctcaaaggc gcaccatcgc tttgccttct cgaacagacc ctcatcgctg
    16561 gaatgaattt ttgaagaagg atttttcata tagattaaga aactgcaaaa ttatgtaact
    16621 tcttgttcat atagaaagaa tgttgtgttt aaatttatta gatgtattgg gctgggattt
    16681 tttacttctc ttaaaaaatt cagaaaaact tttctaatct acattttatg cttacctttg
    16741 aataaaatat tccatctcgg atgaaaatct ttgaaatctt cacaaaacac tttaaaccat
    16801 caagatgtat cttgataaaa gacttctttg gttttcataa aaagataaaa aaatatttct
    16861 atgaattctt catttctcca catctatggg tattaaagca atttaagaaa atgaacagag
    16921 cacctactga ttttacttca tttcttatca tcttgttact ttatcttctt tatctctgat
    16981 aagaatgaaa ttactttata attttacttc ctttttgtat tactacacag ttgataccat
    17041 ttcaaacagt ctcttgacta aattttcaat ttatagaaat ctatctttgt aaaatttaaa
    17101 atttttaaac cgatgaagaa aaagaggttt tattattata gcagcgatac tacttatggc
    17161 tgcaggtggt attttattgg g
//
LOCUS       DAOORU010000031        16991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4109_length_16991_cov_5.983408, whole genome shotgun sequence.
ACCESSION   DAOORU010000031 DAOORU010000000
VERSION     DAOORU010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16991
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4109_length_16991_cov_5.983408"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PLF20_03485"
     CDS             <1..793
                     /locus_tag="PLF20_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOX80105.1"
                     /translation="KEDKGRYGISIHSGTPPYQIDWGDGFLMPGIDTVVLGVSPGQHK
                     ITIVDGNGCIYRDNYFVKVLKAPPIHFTFDPDTVYLQKPSVQFYSNIDSLLNVGIDVY
                     NFQWNFGEPDSSRSVEKNPLHTFTSAWQFFQQQIEEYRVRLYAINNDNCDTSIAKFLP
                     LRRPKVEVPNVFTPNADGINDVLIIKVDGKTETEQPSLLRYYERMELVILNRWGRKLY
                     ESKDYRNDWDGGKLADGTYFYVLKCIGRFGEEVYKGSIAIMGSKN"
     gene            806..1822
                     /gene="mnmH"
                     /locus_tag="PLF20_03490"
     CDS             806..1822
                     /gene="mnmH"
                     /locus_tag="PLF20_03490"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HOX80106.1"
                     /translation="MEIPVKNIENFLRIDAPCVDVRSPSEYTHGHIPDAINIPLLDDE
                     VRKQVGIAFVQQGRVSAVKIGISLTSYWLPVIIDEMIRVSLDRQLKIYCWRGGMRSAA
                     AAFLARLAGLNPVLLVGGYKSFRRYVHQSFIFPWKFIVIGGMTGSGKTELLFRLAEMH
                     HQVLDLEGLANHKGSVFGGLMQPPQPTTEQFENLIWQQLSSFNPQFPVWVEDESISIG
                     RCFIPKPIFDRMQASPLIFLDIPSEQRAERLTKEYGQANPEELAAMVWKIRKRLGLQQ
                     TQTITQLILQYRFKEACLMILQFYDKAYSIGLSRRDPHSIIYLPTLEKIDPDKVLNFV
                     DKIS"
     gene            2570..3589
                     /locus_tag="PLF20_03495"
     CDS             2570..3589
                     /locus_tag="PLF20_03495"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX80107.1"
                     /translation="MNSSKSLAGIFLSIIILFSISCTQKKEKASDNQPDTIPMALKSL
                     NERLKKNPQNIESYYERANWFIQNARYGEALKDLHAVMKKDSLNPKLYSLLGDVYLNL
                     GKPIQAEEYYSKALHRDPTYNDALVGLARNYLVLKDYDQTFALTAKAITHNALNPKAY
                     YLAAWAFAEKGDTLSAIKNYLKAIEQDQDFFDAYVQLGILYGKKQPQMAEGFFKNALR
                     IKPNHIHTMYLLALLYQNTGQTDKALSAHQSVLAIDPNYSPSMFALGYIYMADILDFQ
                     TALTWFDRVIQLDEKNAAAYLNRGYCYEQLGIKNKASSDYHRALNLQPNFQKAVEALQ
                     RIGEK"
     gene            complement(3803..4006)
                     /locus_tag="PLF20_03500"
     CDS             complement(3803..4006)
                     /locus_tag="PLF20_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HOX80108.1"
                     /translation="MIQKILTFIIVISAVFWVVYRFYRFSKQNVGPCGGCISKNCSSC
                     PAIDLKAEAERRKNAKSEKSAER"
     gene            complement(4003..4683)
                     /locus_tag="PLF20_03505"
     CDS             complement(4003..4683)
                     /locus_tag="PLF20_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOX80109.1"
                     /translation="MRKESFIFYAMLCAAILLARVPCIGPFFRVINKMVHETGHALIV
                     LLFSGKVYKIELFADLSGTTVSSTKSRFSRIIVSLTGYLFSVLMAWVMFYLLYHQQQR
                     AVIILFTSMSLFCLVFFVRNIYGVIWLLAFTVLNMAALYFLSYEMIYWIAFVFSVLLL
                     GDALASVLFLLWLSIRTPRKSGDAHSLRQLTGIHEAVWALIFVAFALWITYDIIIKFF
                     PAFPSLKV"
     gene            4869..6911
                     /gene="gatE"
                     /locus_tag="PLF20_03510"
     CDS             4869..6911
                     /gene="gatE"
                     /locus_tag="PLF20_03510"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatE"
                     /protein_id="HOX80110.1"
                     /translation="MSKQRFQNQENNKSTLHFTFSAIDAKANYEETRRQVGYVNRKQA
                     TQEDYDRIGFKSGLEVHQQLFTEKKLFCNCPAGLYHADDDYDAEVIRHMRPTLSELGE
                     YDGTALMEFKTRKNIIYRIKYDSACTYEVDDTPPFPMNRQALRIAIEISLLSKLNIVG
                     EVHITRKQYLDGSIPTGFQRTAIVGVEGEIPLRHKKIRLIQLSVEEDACREISDIGHF
                     RTFKTDRLGMPLIETVTYPDCTNPDEVMEACNYIRFLNRSTGHVRTGSGAGREDVNVS
                     CRGGHRVEIKGVSHTRWIPELTHNESFRQWALLRIRDKLQSRIQKENWKMTYKRLNFF
                     DFHFDLPALILARDNRYRIIAVNLSQFRGILSHFTQPEHCFANEISDRLKVIACIEKP
                     NMTHSESFTPEINEEDWSKICQILQAEENDAQIVFWGPEDDISTALETIEERCWMAFD
                     GVPPETRKSFEDGTTIFERVLPGADRMYPDTDSAPIPLTDEYIEEIQKDLPTEVMVRY
                     NQLVQWKVPQDTFTYIFKKNLYPLIERIYTELDVPARFTGSFLGHTVKYIEGQKGPSS
                     TFQFDRIYDLFEYLKNNHLDLRLAQKMFPEVYDHPGMDFDSVLTILKFKRVSKEDLLS
                     KINFLSKKFDEIAINPQPENKINWIMGQLQETALGNISMFELRNLLESVVQLPEKE"
     gene            7093..8478
                     /gene="gatD"
                     /locus_tag="PLF20_03515"
     CDS             7093..8478
                     /gene="gatD"
                     /locus_tag="PLF20_03515"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF003217.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-tRNA(Gln) amidotransferase subunit GatD"
                     /protein_id="HOX80111.1"
                     /translation="MSEDIFQGYKGRALEILKQFNVRVWGQAEIKTSRGDFKGTVLPR
                     AENDDDLHIVIKIATGYNIGIDVRTIIEMKEIGYKKANYKIPEKQFPFTPGLPKVKLL
                     GTGGTIASRLDYRTGAVIPAFTPGELYGAVPELADICNLETEKLFAVFSENMGPTQYK
                     ALAIAIGKEIEKGVDGIVIGHGTDTLSHTGAALSFMVQNSPVPIILVGSQRSSDRPSS
                     DAALNLMHAMTAAGHGDIAEVLVCMFGPTSDEYGLLHRGTRVRKMHSSYRSTFRTISD
                     TPVAMVTRKGVTPIKKKYNPRRKDRQVIIKPYYDERVTMLYYYPGMHPDILNGIIDMG
                     YKGIIFVGTGLGHVNKELYPAIERAHKLGIHMFMTVQTIWGYVHMFVYDTGRDMMAKG
                     IIPAGNMLPETAYIKLSWVLGQTDDPEEVKRLMLTPINDEITEREPYNGYLVFQGGVP
                     EIEQFLKRVHK"
     gene            complement(8782..8853)
                     /locus_tag="PLF20_03520"
     tRNA            complement(8782..8853)
                     /locus_tag="PLF20_03520"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8818..8820),aa:Glu,seq:ttc)
     gene            9412..10809
                     /gene="lpdA"
                     /locus_tag="PLF20_03525"
     CDS             9412..10809
                     /gene="lpdA"
                     /locus_tag="PLF20_03525"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOX80112.1"
                     /translation="MDCQYDVIVIGSGPGGYVAGIRAAQLGLKTAVVEKAELGGVCLN
                     WGCIPTKALIKSAQVYNYISQASDYGIKIEGSTRLDFEGMVKRSREVAATMSNGIQYL
                     FRKNKVEHIKGFGKINPGHEVEVISEDGTLKNYNARHIILATGARSRELPNLRQDGKF
                     IIGYREALTLPIQPQSMVIIGSGAIGSEFAFFYNSIGTKVTLVEFMPNVVPLEDEEVS
                     KQLERNFKKAGIKVMTESTVEQVEVKDHHCVVTINTKKGEETLDTDIVLSAVGIAPNI
                     EGIGIEETGIRTEKGKIVVDEFYRTNVEGYYAIGDIIPTPALAHVASHEGIICVEKIA
                     GHEPTPLNYGNIPACTYTQPEVASLGITEKVAREAGYEIKTGKFPYSASGKATAAGHR
                     DGFVKVIIEAKYGKILGVHMIGDNVTEVIAEAVVARKLESTGREIIDSVHPHPTMSEA
                     FMEAMAAAYGEVIHI"
     gene            10988..11488
                     /locus_tag="PLF20_03530"
     CDS             10988..11488
                     /locus_tag="PLF20_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOX80113.1"
                     /translation="MKTLIVARHAKSSWDFPQLKDEDRPLLSKGKKRISKIILSLQKE
                     KIARPGLIISSHAVRAFETSRIFASFFQYPENQIQVSQTVYYGNKDHITDLFYSIDDD
                     IDSVMIVGHNPNILAFINQFLLDPLDGLPTSGVVILELPIESWTELAGAKASTIQKIF
                     PWESNK"
     gene            11526..13679
                     /gene="ppk1"
                     /locus_tag="PLF20_03535"
     CDS             11526..13679
                     /gene="ppk1"
                     /locus_tag="PLF20_03535"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HOX80114.1"
                     /translation="MSKKNEYIKSTDNLEDGLFINREISWLQFNERVLQEASDPTTPL
                     LERIKFLGIFSNNRDEFFRVRVATIHRMNMLMQKKELDYRISFDPRKLLSEIYSIVEN
                     QEKRFTAVYQDLTRQMKENGIFIVNEQELTHEEGKYVRSFFNQNIRSYVFPIMLNNVK
                     NLTFLKDENFYLAVEMADTAKKSKTRFALLKVPVHITGRFISLQPPEDKKDSHFIILL
                     DDVIRYCLPDIFSVFGYDHFEAYTVKFTRDAELDIDNDVSKSFIERMEESLKQRKKGV
                     PVRFVYDEKMPLALLDKLKQKMRITEKDTLRAGSRYHNFKDFMDFPKINNPAFYFEPF
                     PPLAHPLLPPNHSILAIMRERDIMLHYPYHSFQYLIDLLREASIDPQVKEIKMTFYRT
                     ARDSSVINSLINAARNGKKVTVFLELQARFEEETNIYWVNRLQEEEVEIIPTIPDYKV
                     HAKLLVIKRMEDDGNELLYCNVSTGNFNESTATVYADDSLFTTDPRVTKDLDKVFTLF
                     KSRYIPPTFENLCVSPFNIRSFIIRQLNNEIKNARSGKEAWAILKANSLVDEEVAIKI
                     YEASQAGVSIKLLIRGICVIKPGIPNLSDNIEALSIVDRFLEHSRVFIFCNGGRNKYF
                     ISSADLMPRNLDHRIEVVCPIFDKGIQKELMQMIQIELNANVKARSLHDGHINEYRKV
                     NSDQRIRSQYEKYYMFRKMLKGNHFKNKTHDRSTE"
     gene            complement(13762..15363)
                     /locus_tag="PLF20_03540"
     CDS             complement(13762..15363)
                     /locus_tag="PLF20_03540"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOX80115.1"
                     /translation="MKKVLLFVVAIIMGFNLQVFAQGMEDFTNFPETGNSYQDGSFVG
                     LDGSTWTYTQCRGDIAIALPTPTLGKNRTPTSSVESGLIPGGIGVLSFDFMQAFSSSV
                     NLDVYVNGVYITSVTSNNEVNIVKNSGDITVNVPGDFRLQFIQHNTSAGQVSIDNMVW
                     TGYTITALPEPTNYPENFSANVGPFSIILNWDDATTGAQVPQAYLIKASTSNNITAPI
                     DGTPVPDDLDLSDGSGAKNIVQGVETFTFTGLANNQVYYFRIYPYTNTGSLIDYKTDG
                     NAPFASASTLYMSLIHSQDFNDRTFGSWTAVSVIGDQQWTIDTIHGIGNTPCARISGY
                     ATTSNANEDWLISPKLNFANYTSEIIQFYSAKNYTGPDIQVMVSTDYSGLGNPNLAYW
                     ENLDAVLSPGGWAWTFSENVDIANFASGNLYTAFKYTSTATESATWEIDDIKIWGHQE
                     IGIEQTEIHQISISPNPAREFVKISIQHPSELLLYSLTGEKLLQIHLSQGTNSLNLQD
                     YPSGIYIIKLVPEGYPPEVYKLIIR"
     gene            complement(15599..>16991)
                     /locus_tag="PLF20_03545"
     CDS             complement(15599..>16991)
                     /locus_tag="PLF20_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOX80116.1"
                     /translation="QITDLEPITTYYVRAYATNTYGTAYGNEETFTTLTAPYPPIIDF
                     VASSTNINVGNTVNFTDLSTNGPTSWSWSFVGGIPPSSNQQNPSGILYSYPGEYTVCL
                     TASNQYGSNTLCKDAYINVHAPIEAKIVITEIMYNPPETGTDSLEFIELFNNGASAID
                     LEGFYFSEGVTYTFPSYLLAPGAYAVVAVNSQAMQNTFGISTLQWSSGALNNSGELIR
                     LKDKYGYTVDSVYYDDALPWDTLADGWGPSLTLCNPDLDNSLAENWTHSMEFVTVNAN
                     GDSIWATPGGPCIIPVLPVADFVGNPLSLMTGESVQFTDLSTGPNITNWEWIFDGGTP
                     ATFSGQNPPPIYYYESGLYDVTLKVTNEFGTSTAFKPEYITVLIDAIGDDISLIPISI
                     QPNPSTGKVNIKHNQGKLLLEVFNLVGEKVYSQELNPLTQDVDLTPLQKGIYFMRFTN
                     DNGVKLTHKLILK"
BASE COUNT         5411 a         3406 c         3405 g         4769 t
ORIGIN      
        1 caaggaggat aagggacgtt atggcatttc cattcattcg gggactcctc cctatcaaat
       61 cgattggggc gatggcttct tgatgcctgg aatagatact gtggttctgg gcgttagccc
      121 aggtcagcat aaaataacca ttgtagatgg gaatggttgt atttatcgcg acaattactt
      181 cgtaaaagtc ctaaaagcac ctcctattca ttttactttc gatcccgata cggtctatct
      241 gcagaaacct tctgtgcaat tctattctaa tatcgattcg cttcttaatg ttgggataga
      301 tgtatataat tttcaatgga attttggtga acccgattcg agccgctctg tcgaaaaaaa
      361 tcccttacat acctttacca gcgcttggca attttttcag caacaaatcg aagaataccg
      421 tgttcgactc tatgctatta ataatgataa ttgcgataca agtattgcga aattcttgcc
      481 gctgcgacgc cctaaagtgg aagtgcctaa tgtttttact cccaatgccg acggaatcaa
      541 tgatgtttta attattaaag tggatgggaa aacggaaacc gagcaacctt cattgctgcg
      601 atattatgaa cgaatggaac tggtgatcct gaacagatgg ggaagaaaac tttacgaaag
      661 taaagattat aggaacgatt gggatggcgg aaagctggcc gatggtacgt atttttacgt
      721 gttgaaatgt atcggcaggt ttggcgaaga agtgtataaa ggcagtatcg ctatcatggg
      781 gagtaaaaat tagcaggtta aggggatgga aatcccggta aaaaatattg agaatttttt
      841 aaggattgat gcaccttgcg tggatgtacg atcaccgagc gaatatactc atggccatat
      901 tccggatgcc attaatattc cattgctcga cgatgaagtt cgcaaacaag tgggcattgc
      961 ctttgttcaa cagggtagag tttctgcagt aaaaattggt atatctctta cttcttactg
     1021 gcttcctgta ataatagacg aaatgataag ggtttcgttg gaccgtcaat tgaaaatata
     1081 ttgttggcgt ggtggaatgc gtagcgcagc agccgctttc cttgctcgat tggctggttt
     1141 gaatcccgtt ttgcttgtcg gaggatataa atcctttcgt cgatatgtcc atcaatcttt
     1201 catattccca tggaaattca ttgtaatagg cgggatgact ggaagtggga aaactgaact
     1261 tttatttcga ttagccgaga tgcatcatca ggtacttgat cttgaagggc tggctaatca
     1321 caaaggatcg gtttttggtg gattaatgca gcctccacag cctactacag aacaatttga
     1381 aaatctaatc tggcaacaac tttcatcatt taatccgcaa tttcctgtat gggtggaaga
     1441 tgagagcata agtataggac gttgtttcat tcccaagcct atctttgacc gaatgcaggc
     1501 ttcaccttta atttttttgg atattccttc tgaacaaaga gccgaaaggc tcaccaaaga
     1561 atatggccag gccaatccag aagaactggc cgctatggtt tggaagatta gaaaacgact
     1621 cggtcttcaa caaacccaaa ccatcactca actcatcctg caatatcgtt tcaaggaagc
     1681 ttgcttgatg atactgcaat tttacgataa ggcttattca attggccttt cacgacgtga
     1741 tcctcatagt attatctatc tgccaacttt agaaaaaatt gatcctgaca aggttttaaa
     1801 ttttgttgat aaaatatctt aaaaatcctt cgccaaactc tgaccttttg agtctctaat
     1861 tgccgacaaa tattatttaa agttctaatt tagaaacgct aatcaaattc tttgctattt
     1921 tttggtcttc gcatccttta atttccactc caaaacgcta acaacaccaa gaaagcaagt
     1981 tcaaattttt gcggcctttg atctctacgt gctaattttt tcttgctaag acactaataa
     2041 tgtaaaagaa atttcaaaaa aagagagatt ttaataatat aaggaaggga gaacggagga
     2101 aaggagaata gagaatggtg atgggggttg gtggttggag atggtacttg ggaagcaagg
     2161 tggattggtg gatggtgatt agtgattgtt gattagaatt tgaaatttga ttgtaatttg
     2221 ggaattggtg gttgttagtt gttaaaagag ggtggatcag cgagaaaaat aaaaatgtta
     2281 tcgcagatat cgcagaaaca agccttcgca gatgctcgca gaaaaatatc aacatgaatc
     2341 agcgatataa atctgcgtca atcagcgaga aaaaatatag ttctttcgac tttggcggcc
     2401 cccaagtctc cccgtctcac catctccccc tcccctcgtc tcccagtccc caagtctcaa
     2461 aatctcccta ccttcaaatc tcatcattga ttgaataata accaaatgat gttttcatta
     2521 cctgttacaa aaactctaat tttgctggtt caaatcacat tctgccttta tgaattcgtc
     2581 gaaaagcctt gcaggaattt ttctttccat aataatcctt ttttcaattt catgtacaca
     2641 aaaaaaggag aaagcatcgg ataaccagcc cgatacaatc cctatggctt tgaaaagttt
     2701 aaatgaacgc ttgaagaaaa atccacaaaa tatagaatca tattacgaaa gagctaactg
     2761 gttcatacaa aatgcaagat atggcgaagc tcttaaagac ctccatgcag tgatgaaaaa
     2821 agattctctg aatccaaaac tatacagttt gttgggtgat gtgtatctta accttgggaa
     2881 acccattcag gctgaagaat attattctaa ggctcttcac cgcgatccta catacaatga
     2941 tgctttggta ggattagcca gaaactatct ggtactgaaa gattacgatc aaacattcgc
     3001 ccttactgct aaggctatta cgcataatgc cttgaatcca aaggcttact atctggcagc
     3061 ctgggcgttt gccgaaaaag gagatacatt gagcgccata aaaaattatt tgaaagccat
     3121 agaacaagat caggattttt ttgatgccta tgtgcaatta ggaattcttt atgggaaaaa
     3181 acaaccccaa atggctgagg gattttttaa aaatgccctg cgcatcaaac ccaaccatat
     3241 tcatacgatg tatttactcg cactgcttta tcaaaataca ggacaaaccg ataaggctct
     3301 atctgcccat caatcggttc tggccatcga tcctaactat tctccttcca tgtttgccct
     3361 tggttatatt tatatggctg atatactcga ttttcaaacg gcacttacgt ggttcgaccg
     3421 cgtgattcaa ctcgacgaga aaaatgctgc agcatatcta aaccgtggat attgctatga
     3481 acaacttggc attaaaaaca aagcttcgtc cgattatcac agggctctaa accttcaacc
     3541 caactttcaa aaggcagtag aagcccttca acgaattggt gaaaaataac cttaagaatt
     3601 agagataact ctcacgtacc tcggtcagtt cggcgattaa atgctcacag actttctcca
     3661 atagaaataa atcttcatct aagccagaac cattcgatga tcttcctttt ttctcgagcc
     3721 gaaaagccaa gcagcgagca ttttcatcat aaaaattgac ataattccct tgatgctatg
     3781 cgcatttctg gcaagatcga ttttatcgtt ctgcagattt ttccgatttg gcgttctttc
     3841 ggcgttcggc ttctgctttc aaatcaatgg ctgggcatga gctgcaattt tttgaaatgc
     3901 aacctccaca ggggcccaca ttttgtttag aaaatctata gaaacgataa accacccaaa
     3961 agactgccga aataactatg atgaaggtaa gaattttctg tatcatactt tcaatgaagg
     4021 aaaagcagga aaaaatttga taatgatgtc gtaagttatc cacaaggcga atgccacaaa
     4081 gatcaaagcc catactgcct cgtgaattcc cgtaagctgc ctcaggctgt gggcatcgcc
     4141 gcttttacga ggcgttcgaa tactgagcca gaggagaaac aacactgagg ccaaggcatc
     4201 acccaacaaa agtacactaa aaacgaatgc aatccagtaa atcatctcat acgacagaaa
     4261 atataaggcc gccatattaa gaacagtaaa cgcgagtaac cagattactc cataaatatt
     4321 cctgacaaaa aataccaaac aaaacagact catggatgta aaaagtatga ttacagccct
     4381 ttgctgctga tgatacaata aatagaacat tacccatgcc atcaaaacag aaaaaagata
     4441 tcctgtcagc gacacaatga ttcgtgaaaa cctactcttt gtgctcgaga cggtagttcc
     4501 cgataaatct gcaaataatt cgatcttata gactttgcca ctgaataaaa gcactatcag
     4561 ggcatgaccc gtttcgtgta ccatcttatt gataactctg aaaaaaggtc cgatgcaggg
     4621 aacacgagcg agaaggatgg ctgcgcaaag cattgcataa aaaatgaaag attcttttct
     4681 catgatcgaa ctttctatac gcaaaattaa ggagagctca aaaaaaatct cgaggaaaag
     4741 gtaaaagaaa tgaaattcat aatgtatttt ctgtgagtgt tttcgatcgg cttctcatgc
     4801 ttttcattgc gaattccttg tggtcagatt gagaattcct acatttgaag tctcaaacgt
     4861 cttttctaat gtcaaaacaa cgttttcaga atcaagaaaa taacaaaagt acgctgcatt
     4921 ttactttttc agccatcgat gcaaaagcca attatgaaga aactcgtcga caggttggtt
     4981 atgtaaaccg taagcaagcc actcaggaag actacgatcg cattggattc aaatcgggtc
     5041 tcgaagttca tcaacaattg ttcaccgaaa aaaaattatt ctgcaattgc ccggcaggcc
     5101 tttatcatgc cgatgatgat tatgatgccg aagtgattcg gcacatgcgg ccaaccttga
     5161 gtgaacttgg tgaatatgat ggtactgcct taatggaatt caaaacgcga aaaaatatta
     5221 tttatcgaat caaatatgac agtgcctgta cgtatgaggt ggacgatact cctcctttcc
     5281 caatgaaccg tcaggctctg agaattgcca tcgaaatttc acttctatcc aaattaaata
     5341 tcgttggcga ggtacatatt acacgaaaac aatatctcga tggttccata cccacaggat
     5401 ttcagcgcac ggccattgtg ggagtggaag gcgaaattcc tttacgtcac aagaaaatca
     5461 ggcttataca actgagtgtc gaagaagacg cctgccgtga aatttccgat attggccatt
     5521 ttcgtacttt caaaaccgat cgtttgggaa tgcccctgat agaaacggta acctatcccg
     5581 attgcaccaa tcccgatgaa gttatggagg cctgcaatta tattcgattt ctcaaccgta
     5641 gcacgggaca tgtaagaacg ggcagtggcg cgggacgtga agatgtaaat gtaagctgca
     5701 gaggaggtca tcgtgtagaa atcaaaggtg tttcgcatac ccgctggatt ccagaattaa
     5761 cgcataatga atcttttcgt caatgggcat tgttgcgcat tcgagataaa cttcaatctc
     5821 gtatccaaaa ggaaaactgg aaaatgactt ataaacgtct gaattttttc gatttccatt
     5881 tcgatttgcc cgccttgatt ttggcccgcg acaatcgtta tcggattatt gcagttaacc
     5941 tttctcaatt tcgcggcatc ctttctcatt tcactcaacc agagcattgc tttgccaacg
     6001 aaatttctga ccgacttaag gtaattgcct gcatagaaaa gcccaatatg acgcattctg
     6061 agtcctttac accagaaatt aatgaagaag actggagcaa aatctgccag atattgcaag
     6121 ctgaagaaaa tgacgctcag attgtttttt gggggcccga agatgatatc tctaccgcat
     6181 tggaaactat tgaagaacgc tgttggatgg cattcgacgg agttccaccc gaaacccgga
     6241 aatccttcga agacggtact actatatttg aacgcgtttt acccggcgcc gaccgtatgt
     6301 accccgatac cgattctgca cctatacctc tgaccgatga gtatatagaa gaaattcaaa
     6361 aagatttgcc cactgaagtc atggttcgtt ataaccaatt ggttcaatgg aaagtacctc
     6421 aagatacatt tacttacatc ttcaagaaga atctttatcc tctcatcgaa cgcatctata
     6481 ccgagcttga tgttccagcc cggtttacag gaagttttct gggacatacc gtcaaatata
     6541 tcgagggaca aaagggtccg tcatccacat tccaattcga ccgcatctac gacctcttcg
     6601 aatacctgaa gaataatcat ctcgatttga gactggcgca aaaaatgttt cccgaagttt
     6661 acgaccaccc tggaatggat tttgactcag tacttacgat tcttaaattt aaacgtgtat
     6721 cgaaagaaga tctcctttcc aaaataaatt tcttatcgaa aaaattcgac gaaatcgcca
     6781 tcaaccctca acctgaaaat aaaattaact ggatcatggg acaacttcag gaaactgctt
     6841 tgggcaatat ttccatgttt gaattgagaa atttactcga atccgttgtt caattgcctg
     6901 aaaaggagta aaacatgaag aactattcat gtattttgct aaattctaag attattaaag
     6961 atatatataa acagtttaaa aatgctaatt tagaaataaa aaaaataata ttcgattttt
     7021 tattttttga aataattgaa aaatggaaaa gctgtcaata ataatcggat caactaaatt
     7081 taattactta caatgagtga agatattttt cagggctata aaggcagggc actcgaaata
     7141 ttgaagcaat tcaacgttag agtatgggga caggctgaaa taaaaaccag tcggggtgat
     7201 ttcaagggta cggtattacc cagagcagaa aacgacgatg atttacacat cgtcataaag
     7261 atagccaccg gctacaatat tgggatagat gttcgtacca ttatcgaaat gaaagagata
     7321 ggttataaaa aagccaatta caagataccc gagaagcaat tcccttttac acctggtctc
     7381 cccaaggtaa aacttttggg tactggcggt acaattgcat caaggctcga ttatcggact
     7441 ggagcggtta taccggcttt tacgcctggt gaactttacg gagctgtgcc tgaattagct
     7501 gacatttgta atctcgaaac cgaaaagctt tttgctgtat tcagtgagaa tatgggccct
     7561 acccaataca aagcgcttgc tatagctata ggaaaagaaa tcgagaaagg agtagatggc
     7621 attgtgatag ggcatggcac agacacgctt tcacatacag gagccgcact ttcgttcatg
     7681 gtacagaatt ctcctgtacc aatcatcttg gttggttcgc agcgttcatc cgacagacca
     7741 agcagtgatg ccgcattgaa cttgatgcat gccatgactg ccgctggcca tggcgacata
     7801 gccgaagttc tagtttgtat gtttggacct accagcgatg aatatggctt acttcatcgt
     7861 ggaaccagag ttagaaaaat gcactcgagc taccgctcca ctttccgcac gattagcgac
     7921 actcccgtag ctatggttac acgtaaaggc gtcactccca tcaagaaaaa atataatcct
     7981 cgtagaaaag atcgccaagt aattatcaaa ccctattatg acgaaagggt taccatgctc
     8041 tattactacc ctggcatgca tcccgacata ctcaacggaa tcattgatat gggctataaa
     8101 ggaattatat ttgtaggaac tggcctggga catgtaaaca aggaacttta tcccgccatc
     8161 gaaagagcac ataaattggg tattcacatg tttatgaccg ttcaaactat atggggctat
     8221 gttcacatgt tcgtttacga tactggacgc gatatgatgg ccaaaggaat cattccagca
     8281 ggcaatatgc tacccgaaac tgcttacatc aaactgagct gggttttagg tcaaaccgac
     8341 gatcccgaag aagtaaaacg tcttatgctt actcctatca atgatgaaat taccgaacga
     8401 gaaccttata atggctatct tgtttttcaa ggtggcgtac ccgagataga gcaattttta
     8461 aaaagagtgc ataaataaaa attcccttcg aataaattcg aatgaagata ttcaaacaac
     8521 tgacaacaaa caactatcaa actccaaatt acaaattaca aacaaattcc aaaatccaac
     8581 taacaattac aaaattccaa attctaatca ccatccacta tccaccaacc accttgtctc
     8641 ttcgtctccc agtcctcatt caccaatcac tatccaccat tctccgttca cctaaattaa
     8701 aacaaaaaca ctcaacacca ttgaagcatc gacaacagaa aacatcaatt atttcagatt
     8761 tctctcttca ccttcatttt gtagcccgta ggggaatcga acccctgtta caaggatgaa
     8821 aaccttgcgt cctaacccct agacgaacgg gccttatatg aaagaactta cttgtttttc
     8881 gggctgcaaa attagaggtt ttatacgaat cccacaatat tcggctaata attttcaaaa
     8941 tattacgaaa cacgcctgaa aactaattca ttagaaattc atccccattt tttcggggga
     9001 atggatcatt ccaaacaatt tattgataat gaatatttta tatttacaaa atttctcttt
     9061 ctattccatt cggctattat attcatttac aaatatttcc tttaatgcct gaaaataagc
     9121 ctttacacag tttttatgaa ttctactgaa atgatttaca cttcgttttt atcggcattt
     9181 cagaaatctc cagccaatga cgatgctttg gtattcttct tttctggctg aaaatataaa
     9241 aactcagagc agtcacaccc taataaaaaa atttacaaac caaatctatt taaagatcgt
     9301 tatattaaaa catatcattt attctagata gcttaatcat ccgatgaaat ttcgcaagga
     9361 tatataaatt tgcaataaaa aacgaactat ctcaaataaa atcaacatat tatggattgt
     9421 caatacgacg taatagtaat aggcagtggg ccaggtggtt atgtggctgg aattcgtgcg
     9481 gctcagttag ggttgaaaac ggcagtagtc gaaaaggcag aattgggagg ggtgtgtctc
     9541 aactggggat gcattcccac gaaagcactt attaaaagcg ctcaggtata taactacatt
     9601 tcccaggcat cggactacgg aatcaaaata gaaggcagta ccaggctcga tttcgaaggt
     9661 atggtgaaac gttcgcgtga ggttgctgct acaatgagca atggcataca ataccttttt
     9721 aggaaaaaca aagtcgaaca cataaaaggt ttcggaaaaa taaatccagg gcatgaagta
     9781 gaggtgattt ccgaagatgg aacattaaaa aattataatg caagacatat tattttagca
     9841 acaggtgccc gttcgcgtga gcttcctaat cttcgtcagg atggcaaatt cattattggc
     9901 tatcgcgaag cattaacttt acctattcaa cctcagtcga tggttattat aggatcagga
     9961 gcaataggtt cggagtttgc ctttttctat aactcgattg gcacgaaagt aactctggtt
    10021 gagttcatgc ccaatgtagt accattagag gatgaagaag tttcaaaaca gctggaacgt
    10081 aatttcaaga aagcaggtat caaggtgatg accgaatcga ccgttgaaca ggtagaagtg
    10141 aaggatcatc attgcgtcgt aaccataaac accaaaaagg gcgaagaaac cctcgacact
    10201 gacattgtat tgtcggcagt agggattgca cctaatattg aaggaattgg catagaggaa
    10261 accggaatta gaacagaaaa agggaaaata gtggtggatg aattttaccg taccaatgtc
    10321 gaaggctact atgccatagg agatattatt cctaccccgg cattagccca tgttgcatca
    10381 cacgaaggca tcatttgtgt ggagaagatt gcaggccatg agcctacacc tttaaattat
    10441 ggaaacattc cagcttgtac atacactcag cccgaagtag cctcgttagg gataacagaa
    10501 aaagtagccc gtgaagccgg atatgagatc aaaacaggaa agttccccta ttcggcctct
    10561 gggaaagcca ccgcagctgg tcatcgtgat ggattcgtaa aagtaatcat agaagcaaaa
    10621 tatggcaaaa tattaggcgt tcacatgatt ggcgacaatg ttactgaagt gattgccgaa
    10681 gccgttgtag ccagaaagct cgaaagtact ggcagagaaa tcatcgacag cgtacatcct
    10741 catcctacca tgtcggaagc ctttatggaa gctatggcag cagcctatgg ggaagtcatt
    10801 catatttgat aaaacatccg tattaatatg ctctgagcat taccttcgtg tggtccactt
    10861 gtcgagcttg ttaaggcaat cattcgtctc tcgaagtttt aaagagtaaa aattttctaa
    10921 agattaattc tttggggcat ttactaaatt tgtaaagatt ctaacattta agaaaaactt
    10981 atagcccatg aaaacactta ttgttgcccg gcatgcaaaa tcctcatggg attttcctca
    11041 gctcaaggat gaggacaggc cattgttgag caaagggaaa aaacgaatca gcaagatcat
    11101 cctatcctta caaaaggaaa aaattgccag acccggtctg atcatttcga gtcatgcggt
    11161 aagagccttt gaaacgtcca gaatatttgc ttcattcttt caatatcccg aaaatcagat
    11221 tcaagtcagc caaaccgttt actacggaaa taaggaccat attaccgatc ttttttattc
    11281 catcgacgat gacatcgaca gtgtgatgat agtcgggcac aaccctaata tccttgcatt
    11341 catcaaccag tttcttctcg atcccttgga tgggttgcca acctcggggg tcgtcattct
    11401 tgaattgccc atcgagagtt ggaccgaatt ggcaggagca aaagccagta caatacagaa
    11461 aatattcccc tgggaaagca ataaataggt ataaacataa ttttatattt tacatcagca
    11521 tggcaatgag taaaaaaaat gagtacataa agtctacaga taatttggag gatggattat
    11581 ttatcaatcg ggaaataagc tggctgcaat tcaacgaaag agttttgcaa gaagcttccg
    11641 atccaaccac ccccttactg gaaaggataa agttcctggg cattttttcc aataatcggg
    11701 atgaattttt tcgggtgagg gtagctacga ttcatcgaat gaacatgctg atgcaaaaaa
    11761 aagaactcga ttatagaatt agtttcgatc ccaggaagtt attgtcagaa atatacagta
    11821 tagtcgaaaa tcaggaaaaa aggtttaccg ctgtttatca ggatttaacc cgtcaaatga
    11881 aagaaaatgg gattttcatt gtaaatgaac aggaacttac gcacgaagaa gggaaatatg
    11941 taagaagctt ctttaaccag aacattcgat cgtatgtttt tcctatcatg ctgaacaatg
    12001 taaagaatct gacttttttg aaggatgaga atttttatct ggcagtggaa atggccgata
    12061 cggccaaaaa gagcaagacc cgatttgctt tactcaaagt ccccgttcat attaccggaa
    12121 ggtttatttc tttgcaaccg cctgaagaca aaaaggattc ccattttatc attcttctgg
    12181 atgatgtgat acggtattgt ttacccgata ttttttctgt cttcggatac gatcattttg
    12241 aggcttatac ggtgaaattt acgcgcgatg ccgagctcga catcgataat gacgtatcga
    12301 agagttttat cgaacgaatg gaagaaagcc tgaaacagcg taaaaaaggc gttccggtga
    12361 gatttgttta cgacgaaaaa atgccgttag cacttctcga taagctaaag caaaaaatga
    12421 gaattaccga gaaggacaca ctaagagctg ggagcagata tcataatttt aaagatttta
    12481 tggattttcc caaaatcaat aatccggcct tttatttcga acctttcccg cccttggctc
    12541 atccgctgct gcctcccaat cacagcattc tggccattat gcgagaacgc gacatcatgc
    12601 tgcattatcc ttatcattcg tttcagtact tgatcgattt gttgcgcgaa gcttccatcg
    12661 atcctcaggt aaaggaaata aaaatgactt tctaccgcac ggcgcgtgat agttcagtga
    12721 ttaattccct gatcaacgcc gcacgcaacg ggaaaaaagt cacggttttt ctcgagttgc
    12781 aggcacgctt cgaagaagaa accaatattt attgggtgaa cagattacag gaagaagaag
    12841 tcgaaattat tcccaccatt cccgattata aggtacatgc caaactcctg gtcataaaaa
    12901 ggatggaaga tgatgggaat gaactgctat attgcaatgt tagtacagga aacttcaatg
    12961 aatcaacggc cacagtttat gcagatgata gccttttcac cactgacccg cgtgttacga
    13021 aagacttgga taaagtattt acacttttca aaagccggta cattcccccc accttcgaaa
    13081 acctctgtgt ttctccattt aatatccgca gttttatcat tcgacagtta aataatgaaa
    13141 taaaaaatgc aagatcgggt aaggaagctt gggctattct gaaggcaaat agccttgtag
    13201 atgaagaagt agcaattaaa atttacgagg caagtcaggc aggtgtcagt atcaaacttt
    13261 taataagagg tatttgtgtg atcaaacccg gtatcccaaa tttgagcgat aacatcgaag
    13321 ctctgagcat tgtagatcgt tttctggaac attcacgcgt atttattttc tgcaatggtg
    13381 gccgcaacaa atacttcatc agctcagccg acctgatgcc ccgtaacctc gaccatcgta
    13441 tcgaagtggt ttgccccatc ttcgacaaag gcattcaaaa ggaactcatg caaatgatcc
    13501 agatcgaact gaacgccaat gtaaaagctc gatcattgca tgatggacat attaacgaat
    13561 accgcaaagt aaattccgat cagagaataa gatcgcagta cgaaaaatat tatatgtttc
    13621 gaaaaatgct caaagggaac cattttaaaa ataagacaca cgaccgcagt acagaataaa
    13681 acagccatta ttacgcacgt atcgtaccag aaagaaaaac cgacctagtt tatcacaaag
    13741 atcggcttcc cttcaaatat tttatcgaat gattaactta tatacttcgg gtggataacc
    13801 ttccggaaca agtttaataa tgtaaatgcc cgatggataa tcttgcaaat tcagagagtt
    13861 tgttccctga gataaatgga tttgcagtaa tttttctcct gtaagagaat ataaaagaag
    13921 ttcggaagga tgttgtattg atatttttac aaattctcta gcagggttag gcgaaatgct
    13981 aatttggtga atttcagtct gctctattcc gatctcctga tgaccccata tcttgatgtc
    14041 atcaatttcc caagttgcac tttcggtagc cgtactggtg tatttaaagg cggtatatag
    14101 attacctgag gcgaaattag caatatcgac gttttctgaa aatgtccatg cccatcctcc
    14161 aggcgagagc actgcatcga gattttccca ataagcgaga ttgggattac ccaaacccga
    14221 ataatcggta gataccatta cttggatatc agggcctgtg taatttttcg cactataaaa
    14281 ttgtatgatc tcgctcgtgt agtttgcaaa attcaatttc gggctgataa gccaatcttc
    14341 attagcgttg cttgtagtag catatccact tattctagca caaggggtgt tcccaattcc
    14401 atgaatggta tctatggtcc attgctgatc acctataaca ctaaccgccg tccaagagcc
    14461 aaaagtacga tcgttgaaat cttgagaatg aataagggac atataaagag ttgaagccga
    14521 agcaaagggt gcattaccat ccgttttgta atcgataagt gaacccgtat tggtataagg
    14581 gtagatgcgg aaataataga cttggttgtt agctaaacct gtaaaggtaa aagtttctac
    14641 tccctgaact atatttttag caccacttcc gtcggatagg tccaaatcat cgggtacggg
    14701 tgttccatct ataggtgcag taatattatt ggatgtagag gcttttataa gataagcctg
    14761 tgggacctga gctcctgtgg tggcatcgtc ccaattgaga attatgctga aagggccaac
    14821 gtttgccgaa aaattctcgg gataattcgt aggttcagga agtgctgtaa tagtatatcc
    14881 tgtccatacc atattatcga tactcacttg accggcgctg gtgttgtgct gaatgaattg
    14941 aagtctgaaa tcacccggaa catttacagt gatgtctcca gagtttttaa cgatatttac
    15001 ttcattattg gaagttaccg atgtaatata gactccgttt acataaacat cgaggtttac
    15061 actggaggaa aaagcctgca taaaatcaaa gctcaaaacc ccaattccac ctggaattag
    15121 tcctgattct accgaagacg tgggggtgcg gtttttccct aaggttggtg taggtaaggc
    15181 aatggcaata tcacccctgc attgcgtgta agtccaagtg cttccatcca agcccacaaa
    15241 gctaccatcc tgataactat tcccagtttc tggaaaattt gtgaaatctt ccataccctg
    15301 agcaaagact tgaagattga agcccataat tattgccacg acgaataata ggactttttt
    15361 cataatctaa ttttttaatt acattacaaa aataaaacga aaagctgaat tcttttacag
    15421 cttaaccaaa atttaactca gtagatgata ctattcagag tttattcact taaagataaa
    15481 tttcatcaaa atgtaatgaa cagaaaatag tatttctgga atttaaaaga ttaaatgaat
    15541 aaaatagcca gagttccatc cgaaccccgg ctatttttat aaataaatgg aatttgattt
    15601 atttaagtat gagtttgtga gtaagtttaa ctccattgtc attagtgaat cgcataaaat
    15661 agataccctt ttggagtgga gtaagatcga cgtcttgagt tagcggattc aattcttgag
    15721 aataaacttt ttctcctacc aggttaaata cttccaataa gagtttgcct tggttatgtt
    15781 taatatttac ttttccagta gaaggattag gctgaattga aatcgggata agagaaatgt
    15841 catcgccgat agcatcaatt aacacagtga tatattcggg tttgaaagct gtacttgttc
    15901 cgaattcgtt agttacttta agggtaacat cataaaggcc agattcgtaa tagtaaatgg
    15961 gtggagggtt ttgacccgag aaggtagcag gtgttcctcc atcgaaaatc cattcccagt
    16021 ttgtaatatt ggggccagtt gaaagatcgg taaattgaac actttctcct gtcatcaatg
    16081 aaagtgggtt accaacgaaa tcggcaacag gaaggacagg tatgatgcac ggcccacccg
    16141 gtgtagccca gatgctatct ccattggcat ttacggtaac gaattccata ctatgcgtcc
    16201 aattttctgc caaggaattg tcgaggtctg gattacagag agtaagagat gggccccagc
    16261 catcagcgag ggtatcccat ggcagggcgt cgtcataata aactgaatcc acggtatacc
    16321 cgtatttatc ctttagccgg atcagttcgc ctgaattgtt caatgcacca gaactccatt
    16381 gcaaggtcga aataccaaat gtattctgca tggcttgaga gtttacagcc acaactgcat
    16441 aggcacccgg agcaagcaag taagaaggga aagtataagt tactccttcc gagaaataga
    16501 atccttcgag atctatggca ctggctccat tattgaagag ttcgataaat tcgaggctat
    16561 cggtacctgt ttcgggagga ttatacatga tttcggttat cacaattttt gcctcaatag
    16621 gggcatgtac attaatatag gcatccttgc acagagtatt gcttccatat tgattcgaag
    16681 cggtcaaaca aacggtatat tctccaggat aagaataaag aattcctgaa ggattttgct
    16741 ggttagacga gggtggaatg cctcccacga aagaccatga ccacgaagta ggtccatttg
    16801 tgcttaaatc ggtaaaatta accgtattcc ccacattgat gttagtgctg gaagccacaa
    16861 aatcaataat aggaggataa ggagcggtga gggttgtaaa tgtctcttca ttcccataag
    16921 ccgttccgta ggtattcgta gcataagctc ttacataata agttgtaatt ggctccaaat
    16981 ctgtaatctg g
//
LOCUS       DAOORU010000032        16906 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4131_length_16906_cov_5.971396, whole genome shotgun sequence.
ACCESSION   DAOORU010000032 DAOORU010000000
VERSION     DAOORU010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16906
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4131_length_16906_cov_5.971396"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="PLF20_03550"
     CDS             <1..620
                     /locus_tag="PLF20_03550"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOX80117.1"
                     /translation="ELLESEGCNMVFAPSVEEMYPQVPTESYSFGLLEQVMEGFYRPG
                     HFNGVAIVVRRLFDILRPHRAYFGEKDYQQLVIIRKMVEQFQLPVETIGCPIVREPDG
                     LAMSSRNRHLAASERAQASFIYQNLVFAQGNAGKLTVKDLKNEIIRRFQNHPSFRLEY
                     FEFSDSSSLQPVDDWNDAANIRAFIAVYLGEVRLIDNIVLKETRV"
     gene            679..1029
                     /locus_tag="PLF20_03555"
     CDS             679..1029
                     /locus_tag="PLF20_03555"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004354715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOX80118.1"
                     /translation="MMIEILKSKIHRATVTAADLNYIGSITIDEDLMDAANIIENERV
                     FIYNINNGQRFETYVIKGKRGSGVIAINGAAARMVAVGDLVIIVSYATIDFEEAKNFK
                     PTIIFPGKNNQLNW"
     gene            1029..2069
                     /locus_tag="PLF20_03560"
     CDS             1029..2069
                     /locus_tag="PLF20_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOX80119.1"
                     /translation="MRKKLLNIFRFLFFLSIGIFFIWIFVRNLTAQERSEIIASFRRA
                     NYFWIAISICIGLASHASRARRWVILMEPMGYKPKYSNVLMAVFVGYFANLALPRLGE
                     LGRCGVLARYEKIPFNKSLGTVITERTLDLIVFILLFILNLVLQIDLIRQYVNEKIYT
                     PLMHSLGSVENVGLLKWAILGAFVLLIVLLIVFRKKFYRFRFVRKLKDMGKGVWDGIW
                     SILKLNRPIEFIFHTLFIWVAYFFMSWIVFLSLPETSGLGLGAGLAVLVFGSIGIILV
                     QGGIGVYPAIVAETLVIYGISSIIGYAMGWLLWATQTIMLIIAGLSSLILLPVKNKVS
                     YGFRIFNTEESI"
     gene            2032..2532
                     /gene="rfaE2"
                     /locus_tag="PLF20_03565"
     CDS             2032..2532
                     /gene="rfaE2"
                     /locus_tag="PLF20_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOX80120.1"
                     /translation="MDLEYLIQKKVFDADSIELQRLLAYWRFKNFKIVFTNGCFDLVH
                     LGHIDYLIRAADLGDILVIGLNSNESARRIKGEGRPIMDQKSRSSLLASFIFVDAVII
                     FEEDTPIELIRTIQPDILVKGADYKPEKIIGSDIVTENGGEVVTLPLVEGYSTSLIEQ
                     KIRQSS"
     gene            2573..3925
                     /gene="radA"
                     /locus_tag="PLF20_03570"
     CDS             2573..3925
                     /gene="radA"
                     /locus_tag="PLF20_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOX80121.1"
                     /translation="MRIKSAFFCSNCGTESPKWLGRCPVCGEWNTYVEEVIHRHSDSI
                     EKKSSSHGAIHIDSIQNIEQPRIPTHYPEFDRVLGGGFVPGSIILIGGEPGIGKSTLL
                     LQVALDMKQYKIMYVSGEESELQLHIRAERLQKNGSQCYIFTDSDLEEVFHQAEQLQP
                     EILIIDSIQTLQTDVIDSPAGSISQVRECTAQLQKYAKTTNVPVVLIGHITKDGALAG
                     PKVLEHIVDTVLQFEGDRNHGYRILRTMKNRFGSTNELGIFEMNANGLRQIINPAELL
                     ISQHPDDISGIAVATIIEGSRPMLAEVQALVATSTFGVPQRSSTGFDYRRMNMILAVL
                     EKRLGFRFSNKDVFLNVAGGIRLDDTAADLAIAAALLSSSEDIPLPIHLCFAAEMGLS
                     GEIRPVTRLDARIAEAQKAGFKKMIISRFALKNSYDKNKEIEIITVDRMDEMIRYIFG
                     "
     gene            3951..6215
                     /locus_tag="PLF20_03575"
     CDS             3951..6215
                     /locus_tag="PLF20_03575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HOX80122.1"
                     /translation="MQTEKLLLIGYDVQAERKEILKRYKRLLTAFHLKPEQAKEDLAL
                     IRKAFEVALDAHKDMRRKSGEPYIYHPLEVARICAEEIGLGATSIAAALLHDVVEDRS
                     DLYSVEDIHRLFGDKIARICEGLTKIKEIFDHTQPSDQAEHYRKLLLTLSDDVRVILI
                     KLADRLHNMRTLQYMRPEKQLKIAAETTYLFAPLAHRLGLYSIKSEMEDLALKFTEPE
                     IYKEISRRMAESDPVRANFAEEFIKPIEKDLKEKGFRYRIMRRNKSVYSIWTKMKKKH
                     IPFDEVYDVFAIRIILDVPINDERSACWQAYSIVTSHYRPKPDRLRDWISIPRANGYQ
                     ALHTTVMSKEGKWVEVQIRSERMDEIAEKGYAAHWKYKGQYYDDKNIDNWLDRIRDML
                     QNGEAGALDFLEDVQGYLFSSEIFVFKLDGELRILPAGSTVLDFAYAINPDEGNHCIG
                     ANVNHKLQNRFYELKSGDQIEILTSKIQEPSEDWLDHVKTSRAKTRIREYIREKKKKF
                     YNVGEQKVLQIFNKLNIPFTDEKLNELLQKTNRANTLDLYYDVAQGILDPKYISSIMA
                     PPSRSWFSLNPFHRSKHNPNSSLSFEIIHHLRKNSELQLLDSNLDQVKYIVADCCKPI
                     PGDDVIGYIDHHQGVVIHRAQCIEAVHLMSTFSHNVVKVKWTEELNLSVMTGIKLDAI
                     DRKGLLHELSELISNEMDINIKSIHFESDNGVSEGIFFLYVVNVDQLNNLINKLKKVN
                     AVQSVTRIDNYSPL"
     gene            6335..6802
                     /locus_tag="PLF20_03580"
     CDS             6335..6802
                     /locus_tag="PLF20_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HOX80123.1"
                     /translation="MNQKNANKATLETVQNFFAEYLQQKGHRRTPERFAILREIYLTD
                     GHFDIESLYLHMKRNKYQVSRATLYNTMDLLLDCGLVSKHQFGSASAVYEKSFKFQQH
                     DHVICYQDGEVKEVLEFCDPRIIEIQKDVERLLDVEIDGHSLIFFGKRKQAEN"
     gene            6988..8286
                     /locus_tag="PLF20_03585"
     CDS             6988..8286
                     /locus_tag="PLF20_03585"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOX80124.1"
                     /translation="MKVDVLVGLQWGDEGKGKIVDVLSPEYDIIARFQGGPNAGHTIE
                     SEGYKFILHTIPSGILNSNTTNIIGNGVIIDPYILRKEIDILKEKADVDIFRNLLVSR
                     RSHLILPTHRLLDAVYESAKGSTKIGSTLKGIGPTYTDKTGRNGIRIGDLASPNFYQK
                     YEDLKNRHLQIASNYSVDLNAITIEGLSFDEYEGLWMESLTTLKQLRVIDCEYYIQRA
                     LWNGKRVLAEGAQGSLLDIDYGAYPYVTSSNTVAPAVCIGLGVPTSYIDKVFGVFKAY
                     CTRVGNGPFPTELNDEIGDFLRKEGHEFGSTTGRPRRCGWLDIPALRYSVMLNGVTHL
                     FLTKTDVLNKLETIRVCDKYRIEDEEIEDFPYDYSEAAPIYVQRKGWTRSLANVSCVS
                     GLPKELGDYLHLIETRVGIPIVMVSTGPDRHETIMVRPIR"
     gene            complement(8367..11531)
                     /gene="infB"
                     /locus_tag="PLF20_03590"
     CDS             complement(8367..11531)
                     /gene="infB"
                     /locus_tag="PLF20_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOX80125.1"
                     /translation="MSEEKLSVRLSKIATEFNVGISTIVEDLAKKGHKIENNPNTRIP
                     AELYDLLRKLYESDKKIKESVEKKSISSIKKEIPSTETRKIKEETSADTESESIIIKE
                     NVIRPPTPPSVKSTIVQPETGSTAFTEKPPIQETIPTTPELETEENSIRSTVQEIIPG
                     PKIVGKIDLEKQKPSKKSTKTATPKVSGKKSPKLQTETPVIEEKKIEEVSTIKEEDEG
                     PPPSLEGKTIQPPTLEQPAEIQTKAEIEEQAGVLESIQKDEPEVQRKIKTETEILEAQ
                     KTRQEVEKIPSSMVKVEPEITEPIPVKKIEPEEKTTKPDNFIPTKVKKLPGPTIIGKI
                     ELEQVKGGKLTGKEPVATSSEVVGKKSKKKKRKRISGESLLEKATTPKSQTPVETHKP
                     VVHKARKRKDKSKDYRGVKEEVKPELTEEEIQKKVKETLSALKSQTSSTSKTAKIRRA
                     KRAAQLEKNARRLEEEEEQKKVLRVTEFVTANELASLMNVPVNEVIKTCLDLGLFVSI
                     NQRIDAETISIIASEFGYEVAFSEEEILEDSEQRFEDRPEDYLPRHPIVTVMGHVDHG
                     KTKLLDYIRNSNVIAGEYGGITQHIGAYEVTLSNGKKITFLDTPGHEAFTAMRARGAK
                     ITDIAIIVVAADDGVMPQTKEAINHALAACVPLVFAINKMDKPTANPTRVKEELASLN
                     ILVEDWGGKYQCQEISAKTGQNVDLLLEKVLLEAELLDLKANPKRPAIGTVIESSLDK
                     GRGYIAKILVQNGTLHQGDIIVAGNVYGKIKAMYNERNQFVKEVGPSSPILILGLSGA
                     PQAGDKFNVFLDDKEAKAVANKRQQLQREMEIRTQKHITLDEIGRRIAIGDFKELNLI
                     VKGDVDGSVEALSDALLKLSTDEVQVNVIHKSVGQVTETDVLLASASNAIIVGFQVRP
                     SANAKKLAEQEQIDIRIYSIIYQAIDEIKAAIEGMMAPKIEEKIVCNLEVKEIFNISK
                     IGTVAGCLVLDGSINRHTKIRLIRDGIVIYTGRLGSLKRFKEDVKEVKTGQECGLNVE
                     GFNDIKIGDIIEGFEEIEVKKSLK"
     gene            complement(11662..12894)
                     /gene="nusA"
                     /locus_tag="PLF20_03595"
     CDS             complement(11662..12894)
                     /gene="nusA"
                     /locus_tag="PLF20_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010261107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOX80126.1"
                     /translation="MEHNLITSFTEFKDYKNIDRETLMRILEDVFRHTLIKKFGTDEN
                     FDIIVNIDKGDLEIWRTLTIVEDGNLTDPNKEIEYSEAIKLEPDFEIGEQVYQEIKLE
                     DFGRREILTIRQNLISKVQEYEKDNIYRKYKDKVGEIITGEVYQIWKKEILVLDDEGI
                     ELSLPKSEQIPHDHYHKGDTIRAVVAKVELRNSQPVIILSRTSPLFLEKLIEQEVPEV
                     YDGLITIKKIVRVPGERAKVAVESYDDRVDPVGACVGMKGSRIHGIVRELRNENIDVI
                     NYTSNPSLFIQRALSPAKVSSIVIDEENKRAEVYMKPDQVSLAIGKGGYNIKLAGKLT
                     GYEIDVYRDSDAEYEDVDLQEFADEIDQWIIDTFKSIGLDTAKSVLNESVEELIRRTD
                     LEEETILEVRKILQAEFE"
     gene            complement(12923..13387)
                     /gene="rimP"
                     /locus_tag="PLF20_03600"
     CDS             complement(12923..13387)
                     /gene="rimP"
                     /locus_tag="PLF20_03600"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HOX80127.1"
                     /translation="MIDSFKILEIIDLYLEGSDKFLVDLKIKPGNKILVFIDGDHGIN
                     IEDCATLSRFIESKLNRDEEDYSLEVSSTGADAPIRLKRQYKRHIGRKFKIILTDKHQ
                     VRGELQRVEPEGIVLNIQETIITEKGKKKIHFIEKFFPFENIEEARIELSFK"
     gene            13616..14767
                     /locus_tag="PLF20_03605"
     CDS             13616..14767
                     /locus_tag="PLF20_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOX80128.1"
                     /translation="MPRILVVDDERAIRNTLREILEYEKYMVDEAQNGQEAIEKVQSN
                     DYDLVLCDIKMPGIDGIEVLDKILEQGETPVIMISGHGTIDTAVEAIKKGAFDYISKP
                     LDLNRLLVSVRNALDRSRLLTETRNLRKRVLKNNQIIGESNAIKNVLAMLERVAPTNA
                     RVLITGENGTGKELVARALHENSPRAHNPFVEVNCAAIPSELIESQLFGHEKGSFTSA
                     IKQRKGDFELANGGTLFLDEIGDMSLSAQAKVLRSLQENKITRVGGEKEIPVDVRIIA
                     ATNKDLKAEIAKGNFREDLYHRLSVIIIHVPSLAERTEDIPLLAKHFMEIISAEMGKP
                     PLEFTPDSLEALKKRKWTGNVRELRNMVERLAILCDRVITAEDVIKYSN"
     gene            complement(14764..15435)
                     /locus_tag="PLF20_03610"
     CDS             complement(14764..15435)
                     /locus_tag="PLF20_03610"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOX80129.1"
                     /translation="MLQRKFEDNYRHKGLRKKLVEEIRRKGITSENVLKAIEEIPRHW
                     FLDVAFLEFAYQDKPFPIGYQQTISQPYTVAFQTQLLDIQPMEKVLEIGTGSGYQACV
                     LSKLGAKVFTIERQKKLYQQTKEFLQAAGFSDIRCFYGDGYKGLPAYAPFDKIIITAG
                     APEIPQALIEQLKPGGRLVVPIGKNSMQIMTLLIKNADGSLTKEEHGFFAFVPMLRNK
                     ANDDL"
     gene            15576..16553
                     /locus_tag="PLF20_03615"
     CDS             15576..16553
                     /locus_tag="PLF20_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOX80130.1"
                     /translation="MNKLKIGVLGAGHLGKIHIRCIREIETYRLMGFYDPDGQTAAKV
                     SEDFSLKSYPDIDSLIEDVDVVDIVTPTVEHFKCASKALRESRHVFIEKPIVATPDEA
                     RELMKLAYEANVKVQVGHVERFNPAFLTAKPFFNRPMFIEAHRLSMFNPRGLDVPVIL
                     DLMIHDLDIVLSLVKSDIKRIHASGVAIISDTPDIANARLEFENGCVANLTASRMSLK
                     NMRKIRFFQHDAYISVNFLDKKVEIVRIKDHDPERQGPFDMVLDMGPGKPAKQFVFEN
                     PPIVPVNAIKMELESFATALIYDDIPPVTIQDGINALNLAYMIMEKINM"
     gene            16683..>16906
                     /locus_tag="PLF20_03620"
     CDS             16683..>16906
                     /locus_tag="PLF20_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80131.1"
                     /translation="MFSIAFLSCEDFEGEQTIPAYLKIDSIALKIVSPSQGSASSKIT
                     DAWVYIDEQLIGAFELPATIPLLYTGKHRLT"
BASE COUNT         4691 a         3377 c         3514 g         5324 t
ORIGIN      
        1 tcgaacttct tgaatctgag ggatgtaaca tggtttttgc accttcggtc gaagaaatgt
       61 atccccaagt tccgaccgaa tcttattcgt ttggattgct tgaacaagtg atggaaggtt
      121 tttataggcc agggcatttt aatggagttg ccattgtagt caggcgttta ttcgatattc
      181 ttcggcctca tcgagcttat ttcggagaaa aggattatca gcaactggtc atcatccgaa
      241 agatggtcga gcaatttcaa ttacccgtcg aaactatagg atgccccatc gtaagagagc
      301 ccgatggatt ggccatgagt tcgagaaatc ggcaccttgc ggcttccgaa agggctcagg
      361 cttcatttat atatcaaaat ctcgtttttg ctcagggaaa tgctggcaaa ttgacggtca
      421 aagatttaaa aaacgaaatc atccgccggt ttcagaatca cccatctttc aggctcgaat
      481 atttcgaatt ttctgattca tcgagccttc agcctgtcga tgactggaat gatgctgcta
      541 acatcagggc atttattgca gtttatctcg gtgaggttcg tctaattgac aatattgtat
      601 taaaagaaac tcgagtttga gttgaatagg gttgaaaaac cttaaaagat ccagaattaa
      661 attttgcttg ttaatcttat gatgattgaa atattgaaat cgaaaatcca tagagctacg
      721 gtaacggctg ccgacttgaa ctatattgga agcattacca ttgatgagga tttgatggat
      781 gcagccaata ttatcgagaa tgaaagggtt tttatttata atatcaataa tggccaacgt
      841 ttcgaaacct atgtaataaa agggaaaaga ggcagtggtg taatagccat caatggtgct
      901 gctgcacgta tggtagcagt gggtgatctg gtaatcatcg tttcctatgc gactatagat
      961 tttgaagaag caaagaattt caaacctacc attatttttc ctggtaagaa taatcaattg
     1021 aattggtaat gaggaagaaa ttactgaaca ttttccgttt tctgtttttc ctcagtatag
     1081 ggattttttt tatctggatt ttcgtacgca accttactgc tcaggaacgc agcgaaatta
     1141 tcgcttcatt tcgtagagcc aattacttct ggattgcaat ttctatttgt ataggattag
     1201 ccagtcatgc ttcgagggct cgacgttggg tcatcctgat ggaacccatg ggatacaagc
     1261 ccaaatattc caacgtttta atggcagtct ttgtgggcta ttttgccaat ctggcacttc
     1321 ctcgcttggg tgaactcggc cgttgcggtg tgctggcacg ttacgaaaaa atccctttca
     1381 ataaatcgct cggcacagtc atcaccgaac gaaccctcga tctgatcgtt tttattcttt
     1441 tgttcattct aaaccttgtt ctgcaaatcg acctcattcg tcagtatgtc aacgaaaaaa
     1501 tttatactcc attgatgcat tcattaggat cggtcgaaaa cgttggtttg ttgaaatggg
     1561 ctattttagg cgcttttgtg cttttaatcg ttttgctgat tgtttttcga aaaaaattct
     1621 atcgttttag atttgtaaga aaactcaagg atatgggtaa aggggtatgg gatggcattt
     1681 ggtctatctt aaaattaaac cggcctatcg aatttatttt tcatactctg tttatctggg
     1741 tagcatattt ttttatgtcg tggatagttt ttctaagctt acccgaaact tccggtttag
     1801 gcttaggtgc tggtttggca gtgctggtat ttggttcgat aggtattatt ctggtacagg
     1861 gcggcatagg tgtatatcca gctatcgtag ccgaaactct ggtaatttac ggcatcagtt
     1921 ccattattgg ttatgccatg ggctggttat tatgggcaac gcaaactatt atgctcataa
     1981 tagctggttt atcatcattg attctattac ccgttaaaaa taaagtatcg tatggattta
     2041 gaatatttaa tacagaagaa agtatttgat gctgactcga tcgagctgca gcgtctatta
     2101 gcttactggc gattcaagaa ctttaagatc gtttttacta atggctgttt cgatctggtt
     2161 catctgggcc acatcgatta tcttatccgg gccgccgatc tgggagatat tctcgttatt
     2221 ggcctcaact ccaatgaaag tgcccggcgt attaaaggcg aaggccgacc cattatggac
     2281 cagaaaagca ggtcatcgct cttggcgagt tttatattcg tcgatgctgt aattattttc
     2341 gaggaagata ctcctatcga acttatacga acgattcaac ccgatattct ggtaaaagga
     2401 gccgactata aacccgaaaa aattattggt tccgacatag ttaccgaaaa tggcggtgaa
     2461 gtggttacgc ttcctcttgt cgaaggatat tctaccagct tgatcgagca aaagattcga
     2521 caaagctcct gaattttttg taatttcggt ggcagatcgc catagggctg ttatgagaat
     2581 caaaagtgca tttttttgca gcaattgtgg aacggaatct ccgaaatggc tcgggcgctg
     2641 cccggtttgt ggagaatgga atacttatgt ggaggaagtg atacatcggc atagtgattc
     2701 aatcgaaaaa aaatcttctt ctcatggggc aattcatatc gactctattc aaaatatcga
     2761 gcagccaaga attccgacac attaccccga attcgacagg gtattaggtg gaggtttcgt
     2821 ccctggttca attatcctca ttggaggtga acctggcatt ggtaaatcta cattgttgct
     2881 gcaagtcgca ctcgacatga aacaatataa aatcatgtac gtatcgggtg aggaatcgga
     2941 gcttcaattg catatacgtg ccgaacggct tcagaaaaat ggttcgcaat gttacatttt
     3001 taccgattca gacttggaag aggtttttca ccaagcggaa caacttcaac ccgaaatcct
     3061 catcatagat tctattcaaa ccctacaaac cgatgtaatc gacagcccag caggcagtat
     3121 ttcgcaggtg cgtgaatgca cagcccaatt acagaaatat gccaaaacca ccaatgttcc
     3181 agtggtattg attggtcata ttacaaagga tggagcactt gcaggaccta aggttctgga
     3241 acatattgta gatacggttt tacaattcga aggcgaccgc aatcatggct atcgcatttt
     3301 acgaaccatg aaaaatcgtt ttggctcgac caatgaactg ggaattttcg agatgaatgc
     3361 taatggactt cgccagatca tcaatccagc cgaactgctt atttctcagc atcctgacga
     3421 tatttcaggg attgctgtcg ctaccatcat cgaaggatca agacctatgt tagccgaagt
     3481 tcaggcactc gtggctactt cgacttttgg tgttccacag cgttcatcga ccggattcga
     3541 ttatcgccgt atgaacatga tattggcagt tcttgagaaa cgtcttggat ttaggttttc
     3601 gaacaaggat gtctttttaa atgtagccgg aggcatccgc ctcgacgata cagccgccga
     3661 ccttgctata gccgccgcct tgctttcctc ttccgaagat attcccttgc ccattcattt
     3721 atgctttgct gccgaaatgg gcctttcagg cgaaatcaga cccgttacac gactcgatgc
     3781 cagaattgca gaggctcaaa aagctggctt caaaaaaatg atcatctccc gctttgcctt
     3841 gaaaaattct tacgataaaa ataaagaaat agaaattatt acagtggata gaatggatga
     3901 aatgattcgg tatatttttg gatgatggta gttttacaag ggggaaaatt atgcaaaccg
     3961 aaaaactctt attgatagga tacgatgttc aggccgaacg gaaagaaata ctgaaacgct
     4021 acaagcgtct tttgactgct ttccatctta aacctgaaca agcaaaagaa gatcttgccc
     4081 tcatcagaaa agccttcgaa gtggcgctgg atgcccataa ggacatgagg cggaaaagtg
     4141 gcgagcctta tatttatcat cccttggaag ttgcccgcat ttgtgccgaa gaaatcggac
     4201 tgggagctac ttctattgct gctgccttgc tccacgacgt tgtggaagac cgctccgacc
     4261 tatattccgt tgaagatatc catcgactct tcggagataa aatagctcgc atctgcgaag
     4321 gtttaaccaa aatcaaggaa attttcgatc acacccaacc ttccgaccaa gccgaacatt
     4381 atcgcaaact tctgcttact ctttccgatg atgtaagagt aatcctcatc aaattggccg
     4441 accggcttca taatatgcgc acccttcaat atatgcggcc tgaaaaacaa ttgaagattg
     4501 ctgccgaaac cacttacctc tttgcaccat tggctcatcg actaggcctt tacagcatta
     4561 agagcgaaat ggaagacctc gccctcaaat ttaccgaacc cgaaatatat aaagaaattt
     4621 cacgacgcat ggccgaatcc gaccccgtcc gagccaactt cgccgaagaa tttatcaaac
     4681 ccatcgaaaa agacctcaaa gaaaaagggt tcagatatag aatcatgaga cgaaacaaat
     4741 cagtctattc tatctggacc aagatgaaaa aaaaacatat ccccttcgat gaagtttacg
     4801 atgtattcgc catacgcatt attctggatg tgcccattaa tgacgaaaga tctgcatgct
     4861 ggcaggctta ttccattgtt acttctcatt accgtccgaa acccgaccgt ttgagagact
     4921 ggatttccat tccacgcgcc aatggctatc aagcattgca tacaacagtg atgagcaaag
     4981 agggcaaatg ggtcgaagta cagattcgta gcgaacgtat ggacgaaatt gctgaaaaag
     5041 gatatgccgc acattggaaa tataaaggac agtattacga tgataagaac atcgacaatt
     5101 ggctcgatcg tatacgtgat atgcttcaaa atggagaagc aggagctctt gattttctcg
     5161 aagatgtgca aggttacctt ttttcgagcg aaatcttcgt gttcaaattg gatggagaac
     5221 ttcgtatttt gcccgccggc agcaccgtcc ttgactttgc atatgcaatc aatcccgatg
     5281 agggaaacca ttgcataggg gccaatgtaa atcataaact ccagaaccgt ttttatgagc
     5341 taaagagtgg cgaccaaatc gagattttga cttcgaaaat tcaggaacca tcggaagatt
     5401 ggttagacca cgtaaagact tcaagggcaa aaacaagaat tcgggaatat atccgagaaa
     5461 aaaagaaaaa attttataac gtgggcgaac aaaaagtcct ccaaatattt aacaaactca
     5521 atatcccttt tacagatgaa aaattgaacg aattactgca aaaaaccaac cgggcaaata
     5581 cactcgatct ttattatgat gtcgcccagg gaatactcga tcccaagtat atttcgtcta
     5641 tcatggcacc ccccagtcgt agctggttct ctcttaaccc gtttcatcgc agcaaacaca
     5701 atcccaatag ctccttgtcg tttgaaatca ttcatcactt acgtaaaaat tccgaacttc
     5761 aacttctcga cagtaatctc gatcaagtta aatatattgt ggcagattgc tgtaaaccaa
     5821 tccctggcga cgatgtcatc ggttatatcg accatcatca gggcgtagtg atccacagag
     5881 cacaatgcat cgaggctgtc catttgatgt cgacatttag ccataacgtg gtgaaagtaa
     5941 aatggacaga agaattgaac ctttcggtga tgacaggcat taagctcgat gccatcgatc
     6001 ggaaaggcct tttacatgaa ttatctgaac ttatttccaa cgaaatggat atcaatatta
     6061 aatcaattca ctttgaaagc gataatggag tttcggaggg tatcttcttt ctgtatgttg
     6121 tgaatgtaga tcaactcaat aatttaataa ataaattgaa aaaggttaat gcagttcaaa
     6181 gcgttacacg aatcgacaat tattcacctt tatagtcaat ctgaaccagg atgaaaatta
     6241 tttaaaatta gttttaataa aaatagtaac tttggttttt taatgaattt gtaattgatt
     6301 aagatagaac atccattgta atatttatcg ggttatgaat caaaagaatg ctaataaagc
     6361 cactttagaa acagttcaga atttctttgc ggaatattta cagcagaaag gacatcgaag
     6421 gactcccgaa cgttttgcca ttttgaggga aatttattta accgatggtc atttcgacat
     6481 agaatcgctc tatctgcaca tgaagcggaa taaatatcaa gttagccgtg caactctcta
     6541 taatacaatg gatttattac tcgattgcgg cctggtttcg aagcatcaat ttggctctgc
     6601 ttctgcagta tatgaaaaat ccttcaaatt tcagcagcat gaccatgtaa tttgctatca
     6661 agacggcgag gtgaaagaag tactcgagtt ttgcgaccct cgtatcatcg aaattcagaa
     6721 agacgttgag cgtctattag acgttgaaat tgatggacat tcccttattt ttttcggaaa
     6781 acgcaaacaa gcagaaaact aatcttaact tttcttattt ttgtgagtat tgttgcggga
     6841 tgatctcatc attatccaat aaaatcttcc cgatttcaaa aaaatgaaag aaaggaagtt
     6901 atattctttt tgtccttact aactttaaaa aaaatcggaa tagtattttg catatacaaa
     6961 cattattcaa cctataaatt atcgatcatg aaagtggatg tattagttgg attacaatgg
     7021 ggggacgaag gaaagggaaa gatagtggac gtattgtctc ccgaatacga catcatagct
     7081 cgttttcagg gcgggcccaa tgcgggacat actatcgaaa gcgaaggata caagttcatt
     7141 cttcatacca taccttccgg aatcttgaat tctaatacga ccaatattat tggcaatggc
     7201 gtaatcatcg atccttatat acttcgaaaa gagatcgata tattgaaaga gaaagccgat
     7261 gtagatatat ttcgcaatct gctggtttcg cgtcgcagcc atcttatttt gcctactcat
     7321 cgacttctgg atgcggttta tgaatcggct aaaggctcca ccaaaattgg ttccactctg
     7381 aaaggcatcg gcccaacata tacagataaa acaggacgca atggaatacg cataggagac
     7441 ttggcatccc ccaattttta ccagaaatat gaggacttga aaaaccggca tcttcagatt
     7501 gcctcgaatt attcggtcga tttgaatgct attaccatcg aaggcttatc gtttgatgaa
     7561 tacgaaggtt tatggatgga gtcattgact acactgaaac aacttcgggt gatcgattgc
     7621 gaatattata tacaaagagc cttatggaat ggcaagagag ttttggccga aggagctcaa
     7681 ggctcacttt tagatataga ctacggagcc tatccttatg taacttcttc caatactgtt
     7741 gcacctgccg tttgtattgg tctgggtgta cccacttcat atatcgacaa agttttcggg
     7801 gttttcaagg cttattgtac aagagtaggg aatggacctt tcccaaccga actaaatgac
     7861 gaaataggcg attttttgag aaaagaaggc cacgagtttg gttcgactac gggcagaccc
     7921 cgtcgctgtg gctggctcga tattccggct ttacgctatt cggtgatgct aaatggagtt
     7981 acacatttat tcctcaccaa aacagatgtc ttgaataaac tggaaactat acgagtttgt
     8041 gataaatacc gcatagaaga tgaagaaatc gaagattttc cttacgatta cagtgaagca
     8101 gctcccattt atgtgcaaag aaagggatgg accaggagtt tggccaacgt ttcatgtgtg
     8161 agtgggctcc ccaaagagct tggggattat ttgcacctga tcgaaactcg tgtgggaatt
     8221 cccatagtaa tggtttcgac tggccctgac cgccatgaaa ccatcatggt aaggccaatt
     8281 cgttaattgg aatgtcggtt aaagaaaaaa tgctcctgac tgttttaccg ggagcatttt
     8341 tttgtttatc ggaggacaag gtttttttat ttcaatgatt tttttacttc gatttcttcg
     8401 aacccttcga taatatcgcc tattttaata tcgttgaacc cttcgacatt taggccgcat
     8461 tcctgacctg tcttcacttc ctttacgtct tctttgaatc gtttgagtga acccagacgg
     8521 cctgtataaa tcacgatgcc atcccggatt aatctgattt tcgtatgccg gttgatgctg
     8581 ccatcgagga cgaggcatcc ggctaccgtc cctatcttgc tgatattgaa aatttccttt
     8641 acctcgagat tacaaacgat tttttcttcg attttgggtg ccatcattcc ttcgatagcg
     8701 gcttttattt catctatagc ttgatagata atggaataga tcctgatgtc tatttgttcc
     8761 tgctcggcta attttttagc attggccgaa ggacgtacct gaaatcccac aataattgca
     8821 ttggaggccg acgccagcaa aacgtcggtt tcggtaacct gacctaccga tttatgaatg
     8881 acatttacct gcacctcatc ggtagataat ttcaacaagg cgtcagaaag agcttcaacc
     8941 gagccatcta catcgccctt tacaatgagg ttgagttctt tgaaatcgcc aatggctatt
     9001 ctacggccaa tttcatcgag ggtaatatgt ttctgtgtcc taatctccat ttcacgttga
     9061 agttgctgac gtttattagc aactgcttta gcttccttat catcgaggaa cacgttgaat
     9121 ttgtcgcctg cctgtggggc accactgagt ccaagaatga gaatgggaga ggagggccca
     9181 acttctttta cgaactggtt gcgttcgttg tacattgcct ttatttttcc atatacatta
     9241 ccggcaacaa taatatcgcc ctgatgaagt gtgccatttt ggacaagaat tttggctatg
     9301 tagccacggc ctttatcgag gctggattca atgaccgtac caatagccgg ccgtttagga
     9361 ttggccttca gatcgagtaa ctcggcttca agcaagactt tttccagaag caagtcaaca
     9421 ttttgaccgg tttttgcaga aatttcctga cactgatatt tccctcccca atcttctaca
     9481 agaatattca aactggcaag ttcttcttta actctggtag gattggctgt aggtttatcc
     9541 atcttattga tagcgaacac caaaggaaca catgctgcta gtgcatggtt gatggcttct
     9601 ttggtttgag gcattacccc gtcgtcggca gctacaacta taatggcaat atcggtaatt
     9661 tttgcaccac gtgcacgcat ggctgtaaag gcttcatgac caggagtatc gagaaaggta
     9721 atttttttcc cattgcttaa tgttacttca taagccccaa tgtgttgagt tataccgcca
     9781 tattctcctg caattacatt tgagttgcgg atatagtcga gaagtttggt tttgccgtgg
     9841 tcaacatggc ccatcacagt aacgataggg tgtcgtggca aataatcttc gggtcgatct
     9901 tcgaatcttt gctctgaatc ttctaagatc tcttcttccg aaaaggctac ttcatatcca
     9961 aattcagaag ctatgatact gatagtttct gcatcaatcc tttgatttat cgagacaaac
    10021 agacctaagt cgaggcaagt tttaattact tcattcaccg gaacattcat caagctggcc
    10081 aattcattgg ctgtaacaaa ttcggtaact ctcaatactt ttttctgttc ttcttcttct
    10141 tcaagccttc tggcattttt ctcgagttgg gcagctcttt tagctcgtct gatcttggcc
    10201 gtttttgatg tagaactggt ttgcgattta agagctgaaa gcgtttcctt tacctttttt
    10261 tgaatttctt cttctgtcaa ttcaggcttg acctcttctt ttactcccct gtaatccttt
    10321 gacttgtctt tcctctttct tgccttatga accacaggtt tatgagtttc gacaggagtt
    10381 tgagattttg gtgttgttgc cttttccagg aggctttcgc cgctgattct tttccttttc
    10441 ttctttttcg atttcttgcc tacgacttct gatgaggtgg caacaggctc cttgccggta
    10501 agtttgcctc ccttgacttg ttcaagttcg atcttcccta taatagtagg gccaggtaac
    10561 ttttttactt tggtaggaat gaaattgtcg ggtttggttg ttttttcctc tggttctatc
    10621 tttttcaccg gaataggttc tgttatttcg ggttcaacct ttaccatgga gctgggaatt
    10681 ttttcgactt cttgcctggt tttctgtgct tcaagaatct cggtttcggt ttttatcttt
    10741 ctttgaactt ctggttcatc tttctgaatg gattccaaaa ctccggcctg ctcctctatt
    10801 tcagccttgg tttgtatttc ggcgggttgt tccagtgtag gtggttgaat tgttttgccc
    10861 tctaatgaag ggggaggccc ctcgtcttct tccttgattg tagaaacttc ctcgattttt
    10921 ttctcttcta tgactggagt ttccgtctgt agttttggag attttttccc tgaaaccttg
    10981 ggtgtagctg ttttagtgct ttttttagat ggtttttgtt tttcgagatc aattttgccc
    11041 acgatttttg ggcctggtat aatctcttga acagttgatc gaattgaatt ttcttcggtc
    11101 tctaactctg gagtagttgg tatggtttct tgtattggtg gcttttcggt aaaagcagta
    11161 gaaccagttt caggttggac aatagtagat tttaccgaag gaggtgtagg aggtctgata
    11221 acattttctt taataatgat actttcactt tcagtgtcgg ctgaggtttc ttcttttatc
    11281 ttcctggttt ctgttgaggg aatttctttt ttaatggagg agatactttt tttctctacg
    11341 gattctttta tttttttgtc agattcatac aatttgcgca ataaatcata taattcggca
    11401 ggaatcctgg tgttgggatt attttcgatt ttatgaccct tcttagccag gtcttcgacg
    11461 atggtactaa tccctacatt aaattccgtc gcaattttac tcagcctcac tgatagtttt
    11521 tcttctgaca taaattttat ttatgatacg ctataatttt tgcaaaatta gtcgattaat
    11581 atgaaatatc gggttattat catatttcta tgggttttgg tgaataggag tgattattac
    11641 taaacatatt cgtaaatttt attattcgaa ttcggcttgc agtatttttc taacttcgag
    11701 aatagtttct tcttcaaggt cggtgcggcg gatcagttct tccacacttt cgtttagaac
    11761 gcttttcgca gtatcgaggc caatagattt aaaggtatcg atgatccatt ggtcaatttc
    11821 atcggcaaat tcttgcaaat caacatcttc gtactcagca tcgctatcac gataaacgtc
    11881 tatttcatag cctgtaagct ttcctgctag tttgatgtta taaccgcctt tcccgatggc
    11941 aagcgaaacc tggtcgggtt tcatgtaaac ttcggctctt ttgttttctt cgtcgatgac
    12001 gatggatgag actttggctg ggcttaaggc tctttggatg aaaaggcttg gattggaagt
    12061 ataattgatt acatcgatgt tttcgtttcg gagttcacgt acaattccgt gaatacgcga
    12121 gcctttcatc cccacacatg cgcctacagg atcgacgcgg tcgtcgtatg actctacggc
    12181 cacttttgcc ctttctccag gtactctcac aattttcttg atcgtaatca gtccatcata
    12241 aacttccggt acttcttgct cgataagttt ttcgagaaac aggggcgatg tacgtgaaag
    12301 gattataact ggctggctat tgcgcagttc cacttttgca actacagcac gtatggtatc
    12361 gcctttatga taatggtcgt gaggtatctg ttcgctttta ggcaacgaaa gctctatccc
    12421 ttcgtcgtcc aacaccagta tttccttttt ccatatctga taaacttctc cggtgataat
    12481 ttctccaact ttgtccttat attttcggta aatgttatcc ttttcgtatt cctgcacttt
    12541 gctgatcaga ttctggcgaa ttgtaagaat ttcacggcgt ccgaaatctt cgagcttgat
    12601 ttcctgatac acctgttctc caatttcgaa atccggctct aattttatgg cctccgaata
    12661 ttcgatttcc ttgttgggat cggtaagatt cccatcttcg actatggtca gagtcctcca
    12721 tatttcaagg tctcccttgt cgatatttac aatgatgtcg aaattctcgt cagtaccgaa
    12781 ttttttaatc aaagtatgac ggaaaacatc ttccaggatg cgcataagcg tttctcggtc
    12841 gatattctta taatctttga actctgtaaa agaggttatt aaattatgtt ccataaactc
    12901 aaaattttat ttcgttgtaa agttatttaa agcttagttc gatgcgggct tcttcgatat
    12961 tttcgaaagg gaaaaatttc tcgatgaaat ggattttttt ctttcctttt tcggtgatga
    13021 tggtttcttg aatgttcagg acaatgccct ccggttcaac tctttgaagt tctcctctaa
    13081 cttgatgttt gtcggtcaag atgattttga attttcggcc gatgtgtcgt ttgtattgcc
    13141 gtttaaggcg aatgggtgca tcggcgcctg tcgacgaaac ttccaaagag tagtcttctt
    13201 cgtcacggtt tagtttcgat tcgataaacc gacttagtgt ggcacaatcc tcaatattta
    13261 tgccatgatc accatcgata aatacaagga ttttattccc cggtttaatt ttcaaatcaa
    13321 ccaggaactt gtcgctccct tcgaggtaca aatcaatgat ttccaatatt ttgaaactgt
    13381 caatcacttg tgaattatta ttttagaaat aaaaattggg gattgaatcc ccacctttat
    13441 tttgcatttt ccatgcgcat aaatcgtttg caaaattagt atattttaaa attaatcaaa
    13501 attattagta tagaatgaca tgaaattttt gcaagtataa attcttatta aacagtgaat
    13561 aattaaacgg atattgatag attgagtaag tttgccatga aaattgactt gagaaatgcc
    13621 aaggatttta gttgtagatg atgagcgtgc catccgcaat accttgaggg agattcttga
    13681 atatgagaaa tatatggtgg atgaagcgca aaatggacag gaagccattg aaaaggttca
    13741 atccaacgat tatgatttgg ttttgtgcga tattaagatg cccgggatag atggcataga
    13801 ggtattggat aagatattag aacaaggtga aacgcctgtt atcatgatat cgggtcatgg
    13861 cacaatcgat actgcagtag aagcgattaa gaaaggtgcc ttcgattata tcagcaaacc
    13921 tctcgatttg aatcgattgc ttgtcagtgt aaggaatgcg ttggatcgct cgagattgct
    13981 caccgaaact cgaaatctca ggaagagggt tttaaagaat aatcaaatca taggcgaatc
    14041 gaatgccata aagaatgttc tggccatgtt agagagagta gctcctacca atgctagagt
    14101 attaattacg ggagaaaacg gtactggtaa agaacttgta gccagggcat tacacgaaaa
    14161 tagtcccaga gctcataacc catttgtgga ggtgaattgt gctgctattc cttctgaact
    14221 tatcgaaagc caattatttg gacatgaaaa gggatctttc acttcggcca tcaagcaaag
    14281 gaaaggagat ttcgaactgg caaatggagg aactttgttt ctggatgaaa taggcgatat
    14341 gagcttgtcg gctcaggcca aggtgttgag gtcgctacag gaaaataaaa ttacacgtgt
    14401 aggaggagaa aaggaaattc cggtagatgt acgaataata gcagctacca ataaggattt
    14461 gaaagcggaa attgctaagg gaaactttag agaagatctt tatcaccgtt tgagtgtcat
    14521 catcattcat gtaccttcgt tagccgaacg aacagaggat attcctttgc tagcaaagca
    14581 ttttatggaa attatttcgg ccgaaatggg taaaccaccc ctcgaattta cccctgattc
    14641 cctcgaagct cttaaaaaac ggaaatggac tgggaatgtg cgtgaattga gaaatatggt
    14701 agaaaggctg gctatattgt gcgatcgtgt gattacggct gaagatgtaa taaaatattc
    14761 caattagaga tcatcgtttg ccttatttct cagcatagga acaaaggcaa aaaaaccatg
    14821 ttcttctttc gtaagacttc catcggcatt cttgataagc aatgtcatga tttgcatgga
    14881 atttttacct atgggtacca ctaaccttcc tccaggcttc aactgctcga taagtgcctg
    14941 gggtatttcg ggagctccag ccgtaataat tatcttgtcg aatggagcat aagcaggtaa
    15001 gcctttatat ccatcgccat aaaaacaccg tatatcagaa aatcctgccg cttgcaagaa
    15061 ctccttggtt tgttgataaa gtttcttttg gcgttcgata gtaaaaactt ttgctccaag
    15121 tttcgataaa acgcaggctt ggtatccaga acccgtccca atctccagaa ccttttccat
    15181 gggctgtata tcgagaagtt gagtttgaaa tgccactgta taaggttgcg atatggtttg
    15241 ctggtatcca atcggaaagg gtttatcctg ataggcaaat tcgagaaatg ccacatcgag
    15301 aaaccaatga cgtggaattt cttcgatagc ttttaataca ttttcactgg taatgccttt
    15361 tcttctgatt tcttcgacca gttttttcct caaaccttta tgacgataat tatcttcgaa
    15421 ttttcgctgg agcatatttt actttgttcg cactgcgaaa ttacgttttt agaatgaatt
    15481 taagcattcc ttattcgtta attcttgggc tattttggag atgagatgat ttttcctacg
    15541 tttgcaggtt ttatcttcga taggggaaat aattcatgaa taagttgaaa ataggtgttt
    15601 taggtgcagg tcatctgggg aagattcata ttcgttgcat acgagaaata gaaacttatc
    15661 ggttgatggg tttttacgat ccagatggtc aaaccgctgc gaaggtttcg gaagattttt
    15721 cgttaaagtc gtatcctgac atcgattcgt tgatagaaga tgttgatgta gtcgatattg
    15781 taactcctac ggtcgaacat ttcaaatgcg cttcgaaagc gctaagagag tcgcggcatg
    15841 tatttatcga aaaacccatc gtagccactc ccgacgaagc acgcgaactt atgaaactgg
    15901 catacgaggc caatgtgaag gtgcaagtag gacatgttga gcgttttaat cctgcctttc
    15961 tgacggctaa accttttttc aaccgtccta tgtttatcga agctcaccgg ctttcgatgt
    16021 tcaatccccg cgggctcgat gtcccggtca tactcgacct gatgattcac gacctcgata
    16081 ttgttttaag cttagtaaaa agcgatatca aacgcataca tgctagtggg gtcgccatca
    16141 ttagtgatac acccgatatt gctaatgccc gattggaatt cgaaaatggc tgtgtagcca
    16201 atcttactgc cagtcggatg agcctgaaaa acatgcgtaa gattcgtttt tttcagcatg
    16261 atgcttatat ctcggtaaat tttttagata aaaaagtcga aattgtacgt atcaaagacc
    16321 atgatcccga acgtcaaggt cctttcgaca tggttctcga tatgggcccg ggtaaacctg
    16381 caaaacaatt tgtgttcgaa aaccctccca tcgtaccggt aaacgctatt aaaatggagt
    16441 tggaaagctt tgctaccgca ctcatttatg acgacatacc accggtaact attcaggatg
    16501 gtatcaatgc ccttaatctg gcctatatga tcatggagaa aataaatatg taacccgata
    16561 ttttctcaat ttttttattc acatcaataa tattcagcct acaatctgcg ttttcaaaac
    16621 gaacccaagt ttctttttgc ttttttatga gcgttaaatt cctcgatata ttttgtttaa
    16681 tcttgttttc aatcgcgttc ttgtcatgcg aggattttga aggcgaacaa accattccgg
    16741 cttatttaaa aattgacagc atagctttaa aaatcgtatc accttcacag ggaagtgctt
    16801 cgagcaaaat tacagatgca tgggtttata tcgatgaaca gctaattggt gctttcgaac
    16861 tgcctgcaac tattcccttg ctttataccg gcaaacaccg tcttac
//
LOCUS       DAOORU010000033        16744 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4179_length_16744_cov_5.787285, whole genome shotgun sequence.
ACCESSION   DAOORU010000033 DAOORU010000000
VERSION     DAOORU010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16744
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4179_length_16744_cov_5.787285"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(530..1051)
                     /locus_tag="PLF20_03625"
     CDS             complement(530..1051)
                     /locus_tag="PLF20_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80132.1"
                     /translation="MKTLHVIDEILIETTNYLQEIWDLTTQNSELTNNLAQCQENLKS
                     TLETLKKREESLREKQAYIENLEKIKADLESQLLQSQNLCSDLRSQLLEKEASLNQIR
                     KENKNLSNEIAVLQNKIEQAENRIQELNSKNLELESLLHETQENLKNARFEIENLQQS
                     LPPEQLNNDETNE"
     gene            complement(1064..1576)
                     /locus_tag="PLF20_03630"
     CDS             complement(1064..1576)
                     /locus_tag="PLF20_03630"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOX80133.1"
                     /translation="MECPVCHRQGIDSLLQKCPECGADLTPLGRIQKLRQRVKRNYTL
                     MLSLIVILVIANLIGIFQYHSSTSLTKTKLLSDSVTQLIQVNQELSDSLNILKDSLQK
                     LFVAQKEYSKTEESTTLEHIVVKGESLWKIAEKYLGDGSLYSKIARDNFLSDPSQIAE
                     GQKIIIHKNP"
     gene            complement(1783..2790)
                     /gene="amrS"
                     /locus_tag="PLF20_03635"
     CDS             complement(1783..2790)
                     /gene="amrS"
                     /locus_tag="PLF20_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOX80134.1"
                     /translation="MQQALFYERLEDMKVKCLLCPHECLLSEGKIGVCRVRKNISGEL
                     YALNDGIISSANLDPIEKKPLYHFYPGSLIFSIGSYGCNMHCRFCQNWEISQPTDNIF
                     SLRPIVPASEIIKAALNHKENIGIAYTYNEPIVSYEYVIKMATLAKEKGLKNVMVSNG
                     FINQEPLLRILPLIDAWNIDLKAFDESFYKRLTGAQLAPVLETLKTVRKSKAHLEITT
                     LVIPNENDEASKFKKMVNWLADELGEDTILHLSRYFPRYHHQSTITPAHKLVEFYNIA
                     RQRLYWTYVGNIELDIGNNSICPKCKNIVVWRKGYHTAAKGLDESGNCRFCKTSLPFV
                     V"
     gene            2861..3778
                     /locus_tag="PLF20_03640"
     CDS             2861..3778
                     /locus_tag="PLF20_03640"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX80135.1"
                     /translation="MEKTARKKVLGGVAISIAAIMWGLDGIVLTPRLYNLDVAFVVFM
                     LHLIPFILMQFFLFREYTYFKNLSAQSWLALILVALFGGAIGTLAMVKALFLVHFQKL
                     TIVVLLQKLQPVFAITLATFLLKEKLKPNFFIWAAIAIIAGYFLTFGFQLPTISRDPN
                     MLYAAGFSLLAAFSFGSSTVFSKWALKEVRFETATFIRYGLTALILLPYILLTGRFSE
                     LSRVTTLNWILFVIIGLTTGSGAIFLFYFGLRHVKASVSTICELFFPLSAVIFDYLFN
                     HVTLSLVQWISAIVMIFAIVILSLQEKTD"
     gene            complement(3881..5437)
                     /locus_tag="PLF20_03645"
     CDS             complement(3881..5437)
                     /locus_tag="PLF20_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80136.1"
                     /translation="MKSLKLAFILLICALSCFSQNIMISSQNYPEETTIKLNPKNPAI
                     IVAGANLNNYYYSSDTGRTWNRGQLTSNHGVWGDPVIEVDTTGNFFFFHLSNPPSGNW
                     IDRIVCQKSTNNGKTWSDGTFVGLNGKKAQDKQWSVVDRQTNTIYLTWTQFDSYGSSS
                     PSDSSIILFSKSVDEGMSWETPKRINKIAGDCIDSDNTVEGAVPAVGPLGELYVAWAG
                     PGGIWFDRSSDRGQTWLENDIFVDAMPGGWDYDIPGISRTNGFPITKCDLSNSPYHGT
                     IYINWSDQRNGTDDTDVWLSKSIDGGFTWSTPIRVNDDEMGHQQFFTWMDIDQTTGWL
                     YFVFYDRRNYTDNKTDVYLAISADGGETFLNRKISESPFVPVSGIFFGDYTNISVHNN
                     IIRPIWTRLHNGQLSIWTDITPLENLLASTQNTYPTKENGTVEGYPNPSKGIFYVSFK
                     LHAPALVSLDIYDQNGRLAHSIFSQCQFDYGKHIIPIDTKKIGLKPGSYICSLKSKEW
                     KEKQIVMIVL"
     gene            complement(5527..6669)
                     /locus_tag="PLF20_03650"
     CDS             complement(5527..6669)
                     /locus_tag="PLF20_03650"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX80137.1"
                     /translation="MKRFLLTIFIACSVSIAWSQTAKRTTAYNFLRNGKLDKALENIE
                     PCLTHPKTMNEAKTWFYRGNIYLQIGLSEKPEYKALSDNPFQIAYESYQKALTLEGAN
                     EFKLDIIQNMNVIAEGFYVKGVERFNGQDYSSAANLFIEAAKVKEEIGVFDTAAVYNA
                     GLCAELSNDNKLALTQYRKVYEAGLRKPNIYTSLINVSLKSGDTTTAMNYATEGLQLF
                     PEDFSILIAETNIFLAKNMTEQALKNLEIAKSLDQTNPSIFFAVGVSYDRLGNFQKAE
                     EAYLKAIELKPDYFDAIYNLGALYVNKAATTLDEANLLPLEKEKEYNEMKNLAESHLD
                     KAIPFLEKAHQLDPKDRNTIASLKEIYARKGNVEKAKEMDEKLKMQ"
     gene            complement(6754..9330)
                     /gene="gyrA"
                     /locus_tag="PLF20_03655"
     CDS             complement(6754..9330)
                     /gene="gyrA"
                     /locus_tag="PLF20_03655"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOX80138.1"
                     /translation="MEEKQGKVIAVNIENQMKSAYIDYSMSVIVSRALPDVRDGLKPV
                     HRRILYGMYDLGILSNRPYKKSARIVGEVLGKYHPHGDSSVYDAMVRMAQDWSLRYPL
                     IDGQGNFGSIDQDPPAAMRYTEARLRKIAEEMLVDIEKDTVDFQNNFDDSLQEPTVLP
                     AKLPNLLLNGASGIAVGMATNMPPHNLKEVIDGCIAYIDNKDITIAELMNYIKGPDFP
                     TGGIIYGYDGVREAYETGRGRIILRGTADIEEDNDRERIVVTSIPYQVNKAEMIKRTA
                     DLVNEKKIDGISDIRDESDREGLRIVYELKREANANVVLNQLYQHTPLQTSFNVNNIA
                     LVKGRPFTLNLKELIVYFIDHRHNVVIRRTKFQLNEAEKRAHVLEGLLIALDHIDEVI
                     ALIKSSADTEEAKNGLMEKFGLTEIQAKAILAMRLSTLTGLEREKLQQEYQELRRMID
                     YYKEILSNESIRMDIIKKELFELKEKYGDEPKTEIEYTSQDFRVEDTIADDSVVVTIS
                     HLGYIKRTPLREYRRQKRGGQGAKGSDIREEDFVEHLFVATNHNYLLLFTEKGKCFWL
                     RVFEIPEGVKTAKGRPIQNLINLESDDKIRAYINLKNLSDPEYINNNYIILCTRKGII
                     KKTSLEAYSRPRINGINAINIREGDHLLEARLTDGQSEIIMALRSGRAIRFSETKVRP
                     MGRTAMGVRGVRLAHKNDEVIGMICLDSHDYDILVVSEKGYGKRSSLDDYRETNRGGK
                     GVKTLNITDKTGDLIAIINVKDSDDLMIINKNGILIRIPVAELRIQGRATQGVKLINL
                     KGNDEIAAVTRVEPEEEDKNEEEEETSRIHTAIDEMPTPEPEFESGPIESIEDEDNTA
                     ED"
     gene            9655..12213
                     /locus_tag="PLF20_03660"
     CDS             9655..12213
                     /locus_tag="PLF20_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HOX80139.1"
                     /translation="MEAKFSQRVKDVLSYSREEAIRLGNDYIGVEHFFLGMVREGEGM
                     AVKIMQYYGADLNEMKAMIESSILNTSSRPVNTDNIPLIRQAERALKLTYLEAKDLSS
                     EVIGTEHLLLAILKVGGNLVEKALQKYDIGYDEIKAEVKLLSTSDENHLSRKDLPYSD
                     LRRGGQSDDDSDDDREFTNLKRNADPKSKTPVLDNFGRDITKAAEEDRLDPIVGREKE
                     MERIAQILSRRKKNNPVLIGEPGVGKSAIAEGLAMKIIKREVPRSLFNKRIVSLDLGS
                     LVAGTKYRGQFEERMKAVLNELEKNPDVILFIDEIHTLVGAGNASGSLDASNMFKPAL
                     ARGDIQVIGATTLDEYRQYIEKDGALERRFQKVMVDPTTPEETVEILMNIKEKYEDHH
                     LVKYTDEAIHACVYLTMRYITDRYLPDKAIDALDEAGARVHIKNIHVPEEILNIEKNI
                     EEVKQRKSDAINAQKFEEAANFRDLERKLTIELEKAKKRWEENSRIHREIVSEENVAE
                     VVAMMTGIPIQRVAQNEGARLLNMEEDLKRSIIGQDEAIRKVVRAIRRNRAGLKDPNK
                     PIGNFIFLGPTGVGKTHLAKVLAKYLFDSEDALIRIDMSEYMEKFAVSRLVGAPPGYV
                     GYEEGGQLTEKVRRKPYSIVLLDEIEKAHPDVFHLLLQVLDDGVLTDSLGRRVDFKNT
                     IIIMTSNIGSRQLKDFGQGVGFATSAKISQQNENTRSVIENALKRTFSPEFLNRIDDI
                     IIFNNLERDDIHKIIDIEMKQLYERVRNLNYEIVVTTEAKDFLVDKGWDPQFGARPLK
                     RAIQKYVEDALAEEIIRTKLNPGDVIFIDHDKDKDEIVIKTHQVVQPESATGLS"
     gene            complement(13120..13596)
                     /locus_tag="PLF20_03665"
     CDS             complement(13120..13596)
                     /locus_tag="PLF20_03665"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HOX80140.1"
                     /translation="MIQRIQTIYLLIAVIATPILLFFFPLAGYFSDMAVIKLFLTHVQ
                     NLVPSGEMPLKLNPFLQYTGTFIAIIAGAFILYALFTFKNRIKQVKLVRISLFLIILM
                     IAWQFFITDLIKKNLGIIPNYELGIYMPIISLIFLLLAQRAILKDERKVHAADRLR"
     gene            13993..15792
                     /gene="rho"
                     /locus_tag="PLF20_03670"
     CDS             13993..15792
                     /gene="rho"
                     /locus_tag="PLF20_03670"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOX80141.1"
                     /translation="MYNLQELNEKFLPELREIAQSLHIEDIDKLSKMDLIYKIIDHQA
                     ANPSQETLAAEQSRPRRGRPPKNPVLNSPQLNAKEPTKGKRRGRPPKKTEEETTTLPS
                     APVEHTDPPKEPQNTIPPLPDKEAPRRPHVVTMSMKRGEHLNVDFLPEKLQVFDEENG
                     NDNDKEENIASEERLEPEAAVESSLTDKLDYKEIIARERAQREINLRYQRTPFEKYER
                     KRDDFYPFSMEGIVSSEGVLELMPEGHGFLRSSDYNYLPSPDDIYMASNQIKMLGLKT
                     GDTIKGMIRPPRDNEKFFPLVKVELINGLQPEKIRDRVPFDFLTPLFPYKKFKLTGHP
                     DATLTSRIIDMFAPIGKGQRGLIVAQPKTGKTMILKEIANAIAFNHPDVYLIVLLIDE
                     RPEEVTDMQRSVKAEVISSTFDEPAERHVRISGIVLEKAKRLVECGHDVVILLDSITR
                     LARAYNTVSPASGKVLSGGVEANALQKPKRFFGAARQIENGGSLTIVSTALIDTGSKM
                     DEVIFEEFKGTGNMELQLDRKLSNKRIFPAIDIVASSTRRDDLLLPKEVLQRVWILRN
                     HLADMTSQEAMEFLQDKMKFTQSNEEFLASMNG"
     gene            16145..16741
                     /locus_tag="PLF20_03675"
     CDS             16145..16741
                     /locus_tag="PLF20_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80142.1"
                     /translation="MKNAIRISLGIVIIVLCYLIYDSIMTPLRFEKEKNYRYGVIIQK
                     LKDIREIEKMYKNANKKYCGSFDTLFVFAKTAKIPVVRMIPDPTDTTFTRSISDTIGY
                     TLVYDSLFGKREGFRIEDIAINPFNPEVKIKLEAGFIDKGGLKVPVFMASALNVDILK
                     GLDEQLIRNYNASLEALNKFPGLKVGSMTEVSIDGNWE"
BASE COUNT         4970 a         3221 c         3322 g         5231 t
ORIGIN      
        1 gatttcaaag attttcatcc aagatggaat attttattca aaggtaagca taaaaagaga
       61 actatggaaa ggtttcggct aaatttttca gaggaagcaa gcaaaagttt agaaatagaa
      121 atagggagaa tataaaaagc aatattcatt caaaatacaa tttcactgtt cgtttaagga
      181 ccttgctaac attcctttcc ttttcactac cttaacgcaa acaagtattt taaaatggct
      241 ttcttaaaaa agttatgctc aacatcattc aaataaatct gaatttagtt tcgtttgaac
      301 gttgttttta aagctaaggt ttatttgtct atgaagggtt tacattttac aatttaaaca
      361 ttaaaatttt cacttccaat tggttttcac ttcaacaaaa atttattttt gaaacaaaaa
      421 ttccacctat tattgtcctt ttgattaaac tcggatagaa taaccggctt ttttaataat
      481 gattctatcg attgttttct atgaaacctc aattaataac aagaataaat tattcatttg
      541 tttcgtcatt atttaattgc tctgggggaa gagattgctg gagattttct atttcaaacc
      601 gagcattttt gagattttct tgagtttcgt gtaataatga ttcgagctct aaatttttcg
      661 agtttaattc ttgaatcctg ttttcggctt gctctatctt gttttgtaaa actgcgattt
      721 cattggatag atttttgttc tcttttctta tttgattcaa tgatgcttcc ttttcaagaa
      781 gttgtgatcg caaatcggaa cagaggtttt gcgattgcaa gagttggctt tccaagtcag
      841 ctttaatttt ttcgagattt tcaatataag cttgtttttc tcgcaggctt tcttctctct
      901 tttttaaggt ctcgagtgtg gatttcaggt tttcctgaca ttgagccaaa ttatttgtaa
      961 gttcactatt ctgcgtggtt aaatcccata tttcctgcaa gtaatttgta gtttctatca
     1021 gtatctcatc tattacatgg agagttttca tggttattag attttacggg tttttatgaa
     1081 tgatgatttt ttgcccttct gctatttgtg aagggtcact caagaaatta tcgcgggcta
     1141 tttttgaata aagactgcca tctcctaaat atttctctgc aatcttccat agactttcgc
     1201 ccttgactac gatgtgttcg agggtagtgg attcttcagt tttagagtat tctttttggg
     1261 caacaaaaag tttttgtaac gaatctttga ggatgtttaa cgaatcactt agttcttggt
     1321 ttacctgtat taattgggta acagaatcgc tcagtaattt tgttttggtt aaggaggttg
     1381 atgaatgata ttgaaaaatt ccaatgaggt tggctattac taatattaca attagcgaca
     1441 gcatcaaggt gtagtttctt tttacccttt gcctgagctt ttgaatacga cccagagggg
     1501 taaggtcagc tccgcattcg gggcattttt gcaaaaggga gtctatccct tggcgatgac
     1561 aaaccgggca ttccatggta aattttttta gttgaacatc aggaagataa aattaaattc
     1621 ggctataaag atatagatta tttatcaata tatctaagaa attactactc gggaaaaatt
     1681 ctttttttaa tgagtgcttt tctaaaaata agattgttaa ggaggttgga agatttagat
     1741 agaagaatga ttgccgttag tgggaagatg catttttcat cattaaacta cgaaagggag
     1801 agatgttttg cagaacctgc aatttcctga ttcatccaaa ccttttgcag ccgtatgata
     1861 acctttacgc cataccacta tatttttgca tttcgggcat attgagttat tgcctatgtc
     1921 aagttcaata ttgcccacat acgtccagta caaacgttgg cgggcgatgt tgtaaaattc
     1981 gaccagctta tgtgctggtg taatggttga ttgatgatga taccgaggga aatatctact
     2041 aagatgtaaa atagtatctt ctcctaattc gtcggccaac cagttgacca tttttttgaa
     2101 tttagaggct tcgtcatttt cattggggat gaccaaggtg gtaatttcga gatgagcttt
     2161 tgattttcta acggttttaa gggtttcgag cacaggtgca agctgtgcac cggttaaacg
     2221 cttgtagaac gattcatcga aggctttcaa gtcgatattc catgcgtcga tgagaggaag
     2281 aattcgaagt agaggttcct gattgatgaa accgttactc accataacgt ttttcagtcc
     2341 tttttccttg gccagggtag ccattttgat gacatattcg tatgaaacga taggttcatt
     2401 ataggtatag gcaatgccaa tattttcttt atgattcaaa gccgctttga taatctcgga
     2461 ggcaggaaca atgggtcgca aactgaaaat gttatctgtt ggctgagata tttcccagtt
     2521 ttggcaaaac cgacagtgca tattgcatcc ataagaacct atgctaaata tcaatgagcc
     2581 aggataaaaa tgatagagag gttttttttc tatgggatcg agattggctg aagaaataat
     2641 cccatcattt aaagcataga gttcacccga tatatttttt ctgacgcggc atacgccaat
     2701 ttttccttca ctgagcaaac attcatgagg acaaagcaaa cactttactt tcatgtcctc
     2761 gagtctttca taaaataagg cttgttgcat atattttcga atttcaacaa ggctcaaatt
     2821 tatatgaaat tatcgaaata gagtaatttt gctgccagaa atggagaaaa cagcccgaaa
     2881 aaaagtttta ggaggagttg ccatcagcat agctgctatc atgtggggat tggatggaat
     2941 tgtgctaact cctcgccttt ataatctaga tgtggctttt gtagttttca tgcttcatct
     3001 gattcctttt attctcatgc aatttttttt atttcgcgaa tatacatact ttaagaatct
     3061 ttcagctcaa agttggcttg ctcttatttt ggtagctttg tttggagggg ctattggcac
     3121 actggctatg gtgaaagctt tatttctggt tcattttcag aaactaacta tagtagtgct
     3181 attacaaaag ttacagcctg tttttgctat taccttagct accttcttac tgaaagagaa
     3241 acttaaaccg aattttttta tctgggcagc aattgctatt atagcgggtt attttttaac
     3301 atttggtttc caattgccta caataagtag ggatcctaat atgctctatg ccgctgggtt
     3361 ttctttgcta gctgcatttt catttggcag cagcacagta tttagtaaat gggcacttaa
     3421 agaagttcgt ttcgaaaccg ctacttttat ccgttatgga ttaacagccc tcattcttct
     3481 tccttatatt ctgttaacag gtcgcttctc tgaactttcc agagttacaa ccctcaattg
     3541 gattcttttc gtcatcattg gactgacaac gggttcggga gctatcttcc tcttctattt
     3601 tggcttgcgt catgtaaagg cttccgtatc tactatatgt gagttgtttt tccctctatc
     3661 ggcagttatt ttcgattatt tatttaatca cgttactctt tcacttgttc aatggattag
     3721 tgccattgtc atgatttttg ccattgtcat actcagcctg caagaaaaaa cagattaaaa
     3781 aatctgtatt aaaattgcga taacaaaaaa taattttatt ggatattttt ctcctattaa
     3841 ttttatgccg aatctagagt tctggagtta ttggtggttt ttaaagcaca atcatcacaa
     3901 tttgtttttc tttccattcc ttcgatttta atgaacaaat gtatgagcca ggtttaaggc
     3961 cgattttttt ggtgtcaata ggaatgatat gtttgccata atcgaattgg cattgggaaa
     4021 aaatggagtg ggcgaggcga ccattttggt cgtaaatgtc gagtgaaacc aatgcaggag
     4081 catgaagttt gaaactaaca taaaaaattc ccttgcttgg attaggataa ccctctactg
     4141 ttccattttc ttttgttggg taggtgtttt gtgttgaggc aagaaggttt tcaaggggag
     4201 taatatcggt ccagatgctg agttgtccat tgtggagtcg agtccagata ggtctaatga
     4261 tattattatg taccgaaatg ttggtataat ctccgaagaa gattcccgaa acgggtacaa
     4321 aagggctttc gcttattttc ctattaagga aggtttcgcc tccatcggca gaaatggcca
     4381 aatacacatc ggtcttatta tcggtatagt tcctgcgatc gtagaaaaca aaataaagcc
     4441 atcctgttgt ttgatcgatg tccatccatg taaaaaattg ttgatgtccc atttcatcgt
     4501 cattcactct tataggggtt gaccatgtga atccaccgtc gatagatttt gatagccaaa
     4561 catcggtatc gtctgtgccg ttgcgttggt cggaccaatt gatataaatt gttccgtgat
     4621 atgggctgtt actgagatca catttggtaa tcgggaatcc gttggtgcgg ctgatgcctg
     4681 gaatatcgta atcccagcca ccgggcatgg catcgacgaa tatgtcattt tcgagccagg
     4741 tttggcctct gtcgctcgag cggtcgaacc aaataccacc ggggccagcc catgccacat
     4801 aaagttcgcc taatggtcct acagcaggta cagcgccttc tacagtatta tcactatcta
     4861 tacaatcacc tgcaatcttg ttgattcttt tgggcgtttc ccaactcatc ccttcatcta
     4921 ctgattttga aaacagtatg atgctgctat cggacgggga gctacttcca tagctgtcga
     4981 actgagtcca tgtcagataa attgtatttg tttgtcgatc tactacgctc cattgtttgt
     5041 cttgagcttt tttcccgttc aatcccacaa aggtaccgtc gctccaggtt ttcccattgt
     5101 tggtagattt ctggcaaact atgcggtcga tccaattgcc tgatggtgga ttggaaagat
     5161 gaaagaagaa gaaatttccc gttgtatcaa cttcgatgac tgggtcgccc caaacaccat
     5221 ggttcgatgt aagttgaccc cgattccatg tacgccccgt atcagagctg taataataat
     5281 tattcagatt ggcacctgcg acaataatgg ccggattttt tggattcaat tttatggtag
     5341 tttcttcggg ataattctgg ctgcttatca tgatgttttg gctaaagcag ctaagggcac
     5401 aaattaacag tataaaagca agtttaagcg atttcataat gaggtactat gataaaaagc
     5461 gaaattaaga aaaaagcctc tatactcaca gaggcttttt aataaaaaag gattttaatg
     5521 gaggggttat tgcattttca atttttcgtc catttctttg gctttttcta catttccttt
     5581 gcgggcgtag atttctttga gtgaggcgat ggtatttcta tctttagggt caagttgatg
     5641 ggctttttcg aggaaaggga ttgctttatc gagatgtgat tcggcgagat tcttcatttc
     5701 gttgtattcc ttttcttttt cgagaggaag cagattagcc tcgtcgagtg tagttgctgc
     5761 cttgtttaca taaagcgctc ctaagttata gatagcgtca aagtaatctg gtttaagttc
     5821 aatggctttt aaataagcct cttctgcttt ctggaagtta ccaagccggt cgtagctaac
     5881 ccccacagca aagaaaatag aggggttggt ttggtcgagg ctttttgcaa tttcaaggtt
     5941 cttcagtgct tgttctgtca tgtttttagc caaaaaaata tttgtttcgg ctatcaaaat
     6001 ggagaaatct tctggaaata attgaaggcc ttcggtagca taattcatag cagtagttgt
     6061 atccccactt ttcagagaaa catttatcaa tgaagtataa atattaggct tacgcaaacc
     6121 ggcttcataa actttccggt attgtgtgag ggctaatttg ttgtcattag acagttccgc
     6181 acagaggcca gcattataga cagctgcggt atcgaaaact ccaatctctt ccttcacttt
     6241 tgcagcttcg ataaacaaat tggctgccga ggagtaatct tgtccattga atctttctac
     6301 tccttttaca tagaatcctt cggctatcac gttcatattc tgaatgatat cgagtttgaa
     6361 ttcattggct ccttccagtg tcagagcttt ctgataactt tcgtaagcta tctgaaatgg
     6421 attatcgctt aatgctttgt attcgggttt ttcgctcaat ccaatttgaa ggtagatgtt
     6481 tccacgataa aaccatgtct ttgcttcatt catggttttt ggatgggtca aacagggttc
     6541 gatgttttcg agagctttat cgagcttgcc attgcgaaga aaattgtaag ctgtcgttct
     6601 tttggcagtt tgactccaag caatacttac actgcaagct ataaaaatcg tcaaaaggaa
     6661 tcttttcatg gtaattaaga attttgtatc aattttcctc tgacaaaatt atgaatttta
     6721 ttcatttcaa atcaaatcgt agaattagaa aaattaatcc tcagcagtat tgtcttcatc
     6781 ttctatcgat tctataggac ctgattcgaa ttcaggttcg ggggttggca tttcatctat
     6841 tgctgtgtgt atcctcgagg tttcttcttc ttcttcgttt ttatcttctt cttcgggttc
     6901 aacgcgggtt actgcagcaa tttcgtcgtt tcccttcaga ttgatgagtt tcacgccttg
     6961 agtagctctg ccttgtattc tcaattcagc aacaggaatg cgtataagta tgccattttt
     7021 attgatgatc atcaggtcgt cggaatcttt gacattgata atagcgatca agtctcccgt
     7081 tttatcggtg atattcagtg ttttaacgcc tttgccacct cggttagttt ccctataatc
     7141 gtcaaggctc gatcgttttc cataaccctt ttccgatacg accagaatat cataatcatg
     7201 gctatcgagg caaatcattc cgattacttc gtcatttttg tgagccagtc gaacacctct
     7261 cactcccata gccgtgcggc ccattggcct tacttttgtt tcggaaaatc gaatggcacg
     7321 acccgaacgc aaggccatga taatttcact ttgcccgtcg gtaagtctgg cttccaacag
     7381 atgatcacct tccctgatgt tgatggcatt gataccattg attcgtgggc gggaataggc
     7441 ttcgagcgag gttttcttga taatgccctt tcttgtacat aaaatgatgt aattgttgtt
     7501 aatatattca gggtcggaga gatttttcag attaatgtaa gcccttattt tatcgtcaga
     7561 ttccagattt atcagattct gaataggcct gccctttgct gtttttactc cctccgggat
     7621 ctcgaaaacc cttaaccaga aacattttcc tttttcggta aacagcaata aatagttatg
     7681 attggtagca acaaacaaat gctccacaaa atcttcttca cgaatatcac ttcctttggc
     7741 tccctgtcct cccctttttt ggcgtctgta ttctcgcaaa ggagttcgtt tgatataccc
     7801 cagatgggat atggttacta ctacgctgtc gtcggctatg gtatcttcta ctctaaaatc
     7861 ctgggaagtg tactctattt cggttttagg ttcgtctcca tatttttcct tcaactcgaa
     7921 cagttccttc tttataatat ccatgcgaat gctttcgttc gagaggattt ccttgtaata
     7981 atcgatcatg cggcgtagtt cctgatattc ttgttgaagt ttttcgcgtt ccaaaccggt
     8041 gagagtcgaa agtctcatgg caaggatagc tttggcctgt atctcagtta gcccgaattt
     8101 ttccataagt ccatttttag cctcttcggt atcggccgaa cttttaataa gggcaattac
     8161 ttcatcgatg tgatcgagag caatgagcaa accttcaagg acgtgtgcac gtttttcggc
     8221 ttcattcaac tggaatttcg ttcttcttat cactacatta tgacgatgat cgataaaata
     8281 gacaataagt tccttaaggt tcaaggtaaa aggtctgccc ttgaccagtg caatattatt
     8341 tacattgaac gaagtctgaa gtggagtatg ctgatataat tgattgagaa ccacattggc
     8401 attagcttca cgtttgagtt catatacaat acgcaatcct tcgcgatccg attcgtcgcg
     8461 tatatcgcta attccatcta ttttcttttc gttaaccaaa tctgcagttc tcttgatcat
     8521 ttcggcctta tttacctgat aagggataga tgtaaccacg atgcgttctc tgtcattgtc
     8581 ctcctcgatg tcggccgtgc ctctcaaaat gattctaccc cgtccggttt cgtaagcttc
     8641 tcttactccg tcataaccat aaataattcc tccggtagga aaatccgggc ctttgatata
     8701 gttcatcagc tcggctattg ttatgtcttt attgtcaata taagctatac agccatctat
     8761 tacttccttg agattatgag gaggcatgtt ggtagccatc ccaacggcaa ttcccgaagc
     8821 accattcaag agtagattag gcaattttgc gggcaaaacc gtgggctcct gcaaagaatc
     8881 gtcgaaatta ttttgaaaat caacggtatc tttttcgatg tcgaccaaca tttcttcggc
     8941 aatcttgcga agccgggctt ctgtataacg catggcggct ggtggatcct ggtctatact
     9001 tccgaaattt ccctgaccat caatcagtgg ataacgaagc gaccaatcct gagccatcct
     9061 caccatggcg tcgtaaaccg acgaatctcc atgaggatga tatttcccca atacttcacc
     9121 gactatacgg gccgattttt tatatggtcg gttggagagt atgcccaaat catacattcc
     9181 atataatatt ctgcgatgaa cgggcttcaa tccgtctctc acatcgggta aggctcttga
     9241 aacaatgacg gacatcgaat aatcgatgta ggccgatttc atctggttct caatattaac
     9301 cgcaataacc tttccttgtt tttcttccat taattttcta aacttttcat ttaatgattt
     9361 ttatcctcta aaaaaaggat gtgcaaaatt agtcattttc ttcgtatcga agaggcccgt
     9421 acgaaacata cttacctatc tgtttgcctg ataaatagtt ccaaactgag aaataaaatc
     9481 tttcacaatg aaaaccagag atcaaaatga ttaattttga aaaaattttt gaaatactga
     9541 aagtttggta gaaaatttgc agttaaccat tacgatttta aaagaacaag ctataatttt
     9601 gcgaaacaat tgcttatgaa tattgttaaa agaaagtaga ggtttaagtt ttatatggaa
     9661 gcaaaatttt cacaaagagt taaagacgtt ttgtcatata gtcgtgagga agcgattcga
     9721 ttaggtaatg actatattgg ggttgaacat ttttttcttg ggatggtacg tgagggcgaa
     9781 ggaatggcag ttaagatcat gcaatattat ggggctgatc ttaacgagat gaaggccatg
     9841 atagaatcat ccatcctaaa tacttcttca aggcctgtga atacggataa tattccattg
     9901 atcagacaag ccgaacgcgc tttgaaactc acctatctcg aggctaagga cttaagcagc
     9961 gaagtgattg gcacagagca tctattactg gccattttga aagttggggg caaccttgta
    10021 gaaaaggctt tacaaaaata tgatattgga tatgatgaga tcaaggctga agtaaagttg
    10081 ttatcgactt ccgatgagaa tcatctatcc aggaaagatt taccttattc cgacttacgg
    10141 cggggtggcc aatcggatga cgattccgac gacgacagag aatttacgaa cctgaaacgg
    10201 aatgccgatc ctaaaagcaa gactccggtt ctcgacaatt ttggacgtga tattaccaaa
    10261 gctgccgaag aagatcgtct cgatcccata gtagggcgtg aaaaagaaat ggaaagaatt
    10321 gcccagatct tgagccgtcg caagaaaaac aatcctgttt tgattggtga gccaggagtt
    10381 gggaaatctg ccattgccga aggattggcc atgaaaatta tcaaacgtga agtaccacgt
    10441 tcgctgttca acaaaagaat cgttagtctc gacctgggtt ctctcgtagc gggtactaaa
    10501 tacagaggac aattcgaaga gcgtatgaaa gccgttttga atgagttgga aaaaaatccc
    10561 gatgtcattc ttttcatcga tgaaatccat accctggtgg gagctggaaa tgcttcgggt
    10621 agcctcgatg cttcgaatat gttcaagcct gctcttgcca gaggtgatat acaagtaatt
    10681 ggagccacta ccttagatga atatcgtcaa tatatcgaaa aagatggggc tctcgaaaga
    10741 cgtttccaaa aagtgatggt cgatcctacc acacccgaag aaaccgtcga gattttaatg
    10801 aacatcaaag aaaaatacga ggatcatcat ctggtaaaat acaccgatga agctattcac
    10861 gcctgtgttt atcttaccat gcggtatatc accgaccgtt atcttcccga taaggccatc
    10921 gatgccctcg acgaagccgg tgcacgcgtg catattaaaa acatacacgt gcccgaagaa
    10981 attctcaata tcgaaaagaa catcgaagaa gttaagcagc gtaaatcgga tgccattaat
    11041 gcccaaaagt tcgaagaagc ggccaatttc agagatctgg aacgcaaact aactatcgag
    11101 ctcgaaaaag ctaaaaaacg ctgggaagaa aatagccgca tccaccgtga aattgttagc
    11161 gaggaaaatg ttgcagaagt cgtagccatg atgacgggca ttcctattca aagagtggct
    11221 caaaatgagg gtgcgcgtct tttaaatatg gaggaagatc tcaagagaag catcatcgga
    11281 caggatgaag ccatacgaaa ggtagtgcgt gccatccgtc gcaatcgcgc cggactcaag
    11341 gatccaaata aacctatagg aaactttata ttcctgggtc ccacgggagt tgggaaaacc
    11401 catctggcaa aggttctggc taaatatctg ttcgattcgg aagatgctct catccgtata
    11461 gatatgagtg aatatatgga gaaatttgcg gtatcacgct tggttggtgc gcctccgggt
    11521 tatgttggtt atgaagaagg aggtcagtta accgagaaag ttcgtcgtaa accttattcc
    11581 attgtcctgc tcgacgaaat cgaaaaggct catcccgatg tatttcatct cctgctccaa
    11641 gttttagatg atggcgtgct caccgacagt ttgggtagaa gagtcgactt caagaatact
    11701 attattatca tgacttctaa tattggttcg cgtcagttga aagattttgg ccagggagtg
    11761 ggatttgcta ccagtgctaa aatttctcag caaaacgaga atacacgcag cgtcatcgaa
    11821 aatgcactga aaagaacatt ctcacccgaa ttcctcaacc gtatagacga tatcatcata
    11881 ttcaataatc ttgaacgcga tgatattcac aagattatcg acatcgaaat gaaacagctc
    11941 tatgaaaggg ttcgtaactt gaattatgag attgtggtta caaccgaggc taaggatttt
    12001 ctggtcgata aaggctggga tcctcaattc ggtgctcgac cactgaaacg cgctatacaa
    12061 aaatatgtgg aagatgcctt agcagaagaa attatacgca ccaaactcaa tccaggcgac
    12121 gtaatcttta ttgaccacga caaggacaaa gacgaaattg ttatcaagac tcaccaagtg
    12181 gttcaacccg aaagtgccac cggattgtcg taattcttca aaacttcaga aagttttcgc
    12241 ttaatagtaa attcgcataa tttttcatcc cccttcggtt tgaatggaat ttcatcaaca
    12301 ctcaaaattc agaaagggga tttttaattg atcacaaatt tttaaaaagc cttcactcga
    12361 tattatatcc cgaaatactc ctttttgccc taattttaga atgaaacccg caagctcttt
    12421 tcttatatcg aataaactat cagctcgatc tgaatccaag cactttcccc atgccagccg
    12481 aaatccccaa cgctcagaca aacatgtaga aagttttaca aaaattgacg taaaattcga
    12541 aaacaaaata aattcagaat ttcgaattga gatgatttga atgaatacat acaggtaagg
    12601 atcaatgata tgttttaaat tcgataataa agaagcataa gataaattat ccacatagat
    12661 ataacaaaag aagtaaaatc tcatgtagat aagaccaatt tcagtaaaac tgacttaacg
    12721 agtgaatgca gggaaaaaac aagacttccg tagaaactat ttttcacgct gtgccatgta
    12781 taaagcaagt ctcccctcaa aatagatcaa tatggctgaa agtgtttcat aacgatttca
    12841 aaaatttcac tgtagatgga acattcagct caaaggcaag catataacga aacttcacag
    12901 ggttatctaa aaatttatta aaaagggatc attagatgtt tcaaacgaag ataattttcg
    12961 tgatatgaat taaaatagac ttgataaagg aaggagttta ttccacaatc tacctgtttc
    13021 tgtgaaagga aaagcaggtt attcgaaaca aggagcgaga aataatgatt cgaaaactcc
    13081 tgtcttgcca gacgatgaaa ttcatttcga agtaaataat cagcgaagcc tgtcggcagc
    13141 atgaactttc ctttcgtctt tcaaaattgc gcgctgcgcc agtaaaagga aaatcagaga
    13201 aatgatgggc atataaatgc ctaattcata attgggtatg atgccaagat ttttcttaat
    13261 caaatcggta atgaagaact gccaagctat catcagaatg atgagaaata aagagattct
    13321 taccaatttt acctgtttga tacggttctt gaaagtaaat aaagcataaa gaataaaagc
    13381 tccggcaata attgcgataa atgttccagt atattggaga aaaggattca attttaacgg
    13441 catttcacct gaaggaacga gattctgaac atgcgtgaga aataatttta tgacggccat
    13501 gtcactaaaa tatcctgcaa gcgggaaaaa gaaaagcagt ataggtgtgg caattactgc
    13561 tatcaacaga taaatggtct gaattcgttg aatcattggt tcaggttttt tgcaaaatta
    13621 aaacgaagat tcaaaagtac tgaatttttg ttgaattact taaaaattct cttgagcatt
    13681 catcagaaaa aatcctattt gatttgaatg gggtatctgt ttaaattttt ttcaaaaaaa
    13741 ggttcaaaat gctgaaaagt ttataaaaac ttcttacctt tgcagcatta aaatttgcct
    13801 cctgcacatc tcggtaaaat ttctaatgat tttttgatat aatatggagg atagaaatat
    13861 attcgattga agcagtggag gttataaata gtttacttct aatatcagtt tttaaatagt
    13921 tttgaagaag agaaggtggt aatatccact gaagaagaaa atatttggct atatactttc
    13981 aacactctta acatgtataa tcttcaagag ctaaatgaaa aatttttgcc cgaattgagg
    14041 gaaatcgctc agtcgttgca tattgaggac atagacaaat tatcgaaaat ggatttgatc
    14101 tacaagatta tcgatcatca ggctgccaat ccatctcaag agactcttgc agccgagcaa
    14161 agccgacctc gacgaggccg gcctcccaaa aatccggttt tgaattcacc tcaattaaat
    14221 gctaaagagc ccactaaagg caaacgaaga ggccggcctc ctaaaaaaac ggaagaagaa
    14281 acaacaactc ttccatccgc tccggtcgaa cataccgacc cacccaaaga gccacaaaat
    14341 acaattccac ccttgcctga caaggaagct ccccgaaggc cccatgtcgt taccatgtcg
    14401 atgaaaaggg gtgagcacct caatgtcgat tttctaccag aaaaacttca ggtttttgat
    14461 gaggaaaacg gaaatgataa cgataaagaa gaaaacattg caagcgaaga aagactcgaa
    14521 ccagaagctg cggttgaatc ttctttaacg gataaactgg attataaaga aattatagcc
    14581 agagaaaggg ctcaaagaga aataaacctg cgttaccagc ggacaccttt cgaaaaatac
    14641 gaacgtaaac gcgatgattt ctacccattc agcatggaag gaatagtcag cagcgaaggt
    14701 gtactcgagc taatgcccga aggacacgga tttctgcgtt cttccgatta caattatctt
    14761 ccttcacccg atgatatcta tatggcttcc aatcaaatca aaatgctggg tcttaaaacg
    14821 ggagatacca taaaaggaat gatcaggcct ccccgagata acgagaagtt ttttcctctg
    14881 gtgaaagtag agttgatcaa tgggttgcaa ccggaaaaga tccgcgaccg tgtccctttc
    14941 gattttctga cacctttgtt cccttataaa aaattcaagc tcactgggca tcccgatgct
    15001 acacttactt cgagaattat cgacatgttt gcccccatcg gcaaaggaca aagaggattg
    15061 atcgtagccc agccaaaaac aggcaagaca atgattctga aagaaattgc caatgccatt
    15121 gccttcaatc atcccgatgt ttatctgatt gtacttttaa tcgacgaacg ccccgaagaa
    15181 gttaccgata tgcaacgaag cgtaaaagca gaagtaatta gctctacttt cgatgaacct
    15241 gctgaacgcc atgtcagaat ttcgggtatc gtcctcgaaa aagccaaacg tttggtagaa
    15301 tgtggacatg atgtggtcat tctgctcgat tcgattactc gtttagcaag agcttacaat
    15361 accgtatcgc ctgcttcggg caaagtactt tcaggaggag tcgaagccaa tgccctgcaa
    15421 aaacccaaac gcttcttcgg tgctgcacga caaatcgaaa atggtggctc actcactatt
    15481 gtgagcactg cactcatcga tacaggttcg aaaatggacg aagtgatctt cgaagaattc
    15541 aaaggcaccg gaaacatgga attgcaactc gaccgtaaac tctccaataa acgcatcttc
    15601 cctgctatcg acattgtcgc ttcctcaacc cgccgcgacg atttactgct gcccaaagaa
    15661 gttctgcaaa gagtgtggat tctacggaat catcttgcag atatgacttc tcaggaagcc
    15721 atggaatttt tgcaggataa aatgaaattt acccagtcca acgaagaatt cctggcttcg
    15781 atgaatggtt gatgactttc gacttattac ctaaatcgta ttccacgaaa tttatcgaag
    15841 aacattcact tctacaaata gaattgcttc atcgaaattt tcgagatgac ttcctacaaa
    15901 acatcaatca cgatgaagtc gatagataga cactcaattt aatcaagcaa aaattcgatt
    15961 gcaattggct tctttggtat cattaaaata agtgttcgaa attttatgtt gataatcaat
    16021 aagttttgaa ttgctaaaaa tcgaattgtc ttctctcaat aaaataattc ttttatcgtt
    16081 atatatcgag tatttttttt tataaaaaac ttctattttt gcatcgcata aaaatttaaa
    16141 tcctatgaag aatgccatca gaatctccct cggaatagtc atcatagttc tatgctactt
    16201 gatctatgac agtataatga ctcctctccg tttcgaaaag gaaaagaatt accgatatgg
    16261 agtgatcatt caaaaactaa aagatatacg tgagatcgaa aaaatgtata agaatgctaa
    16321 taaaaaatat tgtggcagtt tcgacaccct ctttgttttt gctaaaactg ccaagattcc
    16381 cgtagttcgt atgatccccg atcctaccga tactaccttt actcgctcca ttagcgatac
    16441 cattggttat acactggttt acgactcttt attcgggaaa cgggaaggat tcagaataga
    16501 ggatatcgct atcaatcctt tcaatcctga ggtaaagata aaacttgaag ccggttttat
    16561 cgacaaggga gggcttaaag tacctgtgtt tatggcttcg gcgctgaatg tagatattct
    16621 caaaggatta gatgaacagc tcattcgtaa ttataatgca tccttagaag ctttgaacaa
    16681 attcccaggt ctgaaagtag gttcgatgac cgaagtatcc attgatggaa actgggagta
    16741 gacc
//
LOCUS       DAOORU010000034        16724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4190_length_16724_cov_5.295639, whole genome shotgun sequence.
ACCESSION   DAOORU010000034 DAOORU010000000
VERSION     DAOORU010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16724
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4190_length_16724_cov_5.295639"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1899)
                     /gene="groL"
                     /locus_tag="PLF20_03680"
     CDS             complement(262..1899)
                     /gene="groL"
                     /locus_tag="PLF20_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005941147.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOX80143.1"
                     /translation="MAAKEIVYGFEAREALKRGVNALADAVKITLGPKGRNVVLEKSF
                     GAPAITKDGVTVAKEIELKDKIENLGAQMVKEVASKTNENAGDGTTTATLLAQSIYVN
                     GLKNVTAGSNPMDIKRGIDKAVAEVVKSLKSQTQQIGDSLEKIEQVAAVSANNDLFIG
                     KLIAEAMSKVGKEGVITIEEAKGIETSVKVVEGMQFDRGYISPYFVTNTEKMEVEYEN
                     PWILIYDKKISAMKDFLPVLERVIQTGRPLLVIAEDLEGEALATLVVNRLRGTLKVVA
                     VKAPGFGDRRKEMLEDIAILTGGTVISEEKGYKLEDADLSYLGQAARITIDKDNTTIV
                     SGGGEKKQIEARINQIKAQIKATTSDYDREKLQERLAKLAGGVAVIEVGAASEVEMKE
                     RKDRFDDALHATRAAIEEGIVPGGGVAFIRAIEEIRNLKGENEDENFGINIIIRALEE
                     PLRQIAENAGLEGSVVVQKVRDGKDDFGYNARTEQYENLMQTGVIDPTKVARIALENA
                     ASIAGMLLTTECIVSEIKEDKPAPMGGGMPGGMGDMY"
     gene            complement(1955..2224)
                     /gene="groES"
                     /locus_tag="PLF20_03685"
     CDS             complement(1955..2224)
                     /gene="groES"
                     /locus_tag="PLF20_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOX80144.1"
                     /translation="MTLKPLADRVLVEPAEAEQKTAGGIIIPDTAKEKPQKGTVIAVG
                     NGKKDEPLTVKVGDVVLYGKYAGTEINIDGKDYLIMRESDILAIL"
     gene            complement(2449..2817)
                     /gene="secG"
                     /locus_tag="PLF20_03690"
     CDS             complement(2449..2817)
                     /gene="secG"
                     /locus_tag="PLF20_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOX80145.1"
                     /translation="MSTYIFVAVLILIVCLLLTLVVLVQNSKGGGLASNFAGSNQFLG
                     VRRTTDFLEKSTWTLAIALLVLSLFSVAVIPRNVQNKTTKSTAAEALKAQQEAAKKQI
                     DYRPAPGMENFNQPVQPDEQ"
     gene            complement(2821..3741)
                     /locus_tag="PLF20_03695"
     CDS             complement(2821..3741)
                     /locus_tag="PLF20_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80146.1"
                     /translation="MTRTEFIEFLRNPEKISLSSIHELSELTILYPYCQTAQMLLIAS
                     LKAKNDIRYFDRLKVSAAYAGDRSVLRKLISIIETFIPLTCETKSSENQPAKTFSENI
                     NPNLPFIPLRPIETFVPYDAAIDKKQALLASIERKLEQIRQSKIQADKLAREALTYDE
                     KFHSSSSGSPSHTSAISEITSNSSEELVNEPLENNSLSSNLETSSREENWTAPTINEG
                     LQSKMDLIDRFIRDEPRIERKRTSFFNPDDLSEKSNTFTEDIVSETLAKIYAQQGKKE
                     KAIEIYKKLSLLNPEKSAYFAGQIQNLEKE"
     gene            complement(3747..4193)
                     /locus_tag="PLF20_03700"
     CDS             complement(3747..4193)
                     /locus_tag="PLF20_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOX80147.1"
                     /translation="MSYSFTGASIPIEAKTISIDYFPNQASLVVPSLSQTLTEALRNR
                     FITQTPLQLIRRNGDLNIEGSIISYITQPVAIQGTEQAALNRLTISVRVKFTNKFDEK
                     QNFEQTFTRFEDYPFTANLNAVQDNLIQLIVEALVDDIFNKAVVNW"
     gene            complement(4276..5775)
                     /locus_tag="PLF20_03705"
     CDS             complement(4276..5775)
                     /locus_tag="PLF20_03705"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOX80148.1"
                     /translation="MSAAFHKISSIEKYKVKWLYLIMAAFILLNTYLISRDTYWALAI
                     PILVALALLFVFAFDIVILLVAFLTPLSIVLQDFDLGVSISVPSEILLIGLMLFFIIK
                     IIYDSDIDRSFFRHPLSILIIIHLAWMLITCLSSELPLVSFKYLLSQLWFIVPMYFMG
                     ILYFKNEKNIRRFIRVYIASLLIVIVYTTIRHAKVGFDEEIGHWVMYPFYNDHTAYGA
                     ITALLIPVSFSLSLDKSHGRFWQWFAIISTIMLSIGLYLSFSRAAWIGLLGGVIVFIL
                     VLLKIPFKWILTTLILVIGLFFAFQQQIIDRLEKNKQDSSANFVEHVKSIANIRTDAS
                     NLERINRWQAALRLYKERPIVGWGPGTYQFIYAPYQLSKEKTIISTNAGDKGNAHSEY
                     IGPLAERGLLGTILIVLIFSFSIYYGFRVYYKASSRHHRQLAIGIIIGLITYYIHGFM
                     NNFLDTDKLSVPFWAFYAMIVSLDMYYTNKKNSPRSDISTPPLEKPTDS"
     gene            complement(5778..6737)
                     /locus_tag="PLF20_03710"
     CDS             complement(5778..6737)
                     /locus_tag="PLF20_03710"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOX80149.1"
                     /translation="MENYFRNLKLIEILIKWKWHLSIIVVATIVLSVIFSSPSFIKPR
                     YKSSAVLYPSNIKEYSDESLTEQMLQIMQSTDIRDSLIQKFSLIKHYKIDTTKKYWKS
                     NLYWNYNHNVKINKTQYEAVLIEVYDFDPYQARDMVNSIINFYNHKIASLHNEKFNEV
                     VAMYERYLTRKSHELDSLKDLMRDLGVNYNLLDFSFQTQQVVKGYLGTTDFGGGRINT
                     NEVQRIRKNMEEKGGDLIMLQELIRQEAANYALIKKDYDRAYMDHDRQFTYSNIISSP
                     QVPDKKATPVRWIIVLVSTLSVLLLSVIVIGIIENSRLKKENA"
     gene            complement(6830..7717)
                     /locus_tag="PLF20_03715"
     CDS             complement(6830..7717)
                     /locus_tag="PLF20_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80150.1"
                     /translation="MYPVSTSSISKALLSNTYGIQQDVLEFGAEEQAEQMLQILNSNL
                     IRDRIIAKYDLANHYGIKPREKYRLTKLYKEYENNITFRRTENMAIKVSVLDHDPQTA
                     ADIANDIAALFDSTKTAMQRERALKAFHIVEGEYLKKQAEIQQMEDSLVKLRELGVFD
                     YETQTEMINQQLAVEIARGNTRGIQALQAQLDVLARYGGAYVSIRNQLEFEKKQLSDL
                     KAKYEEARVDAFEFLPQKFVVTTAYKAEKKSYPIRWLIVTLTVISSLFLATIIIIVLE
                     NYSSLKAKKKISNPIILSP"
     gene            complement(7968..9458)
                     /locus_tag="PLF20_03720"
     CDS             complement(7968..9458)
                     /locus_tag="PLF20_03720"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOX80151.1"
                     /translation="MQKKFISNLWLLLFLNLLIKPFWILGIDRSVQNLVGFEEYGFYT
                     TILNFSFLFNILLDFGITNYNNRNIAQNRHLLTKHFSNILVLRFLLAALYFVVIMLTG
                     LIIGYNSRQMSLLAVVGFNQFLLALILYLRSNISGLLMFRTDSILSVLDRFLMIVFVG
                     YLLLNQHYRLHFRIEWFVYTQTLAYLLTCLVALMIVWRKAGKTKLHWNFPFFVMIIKQ
                     SIPFAILALLMATYNRIDPVLLERLIPDPQGNHQVGIYAAAYRLLDAFNMVAYLFSVF
                     LLPTFATLIKHKGDIIPFIKFSFSALFVFSILLAFTALYYSPELMQLLYPQHTDESLA
                     EFQARLNQSSLIFTLLMFAFISISSSYVFGTLLTANGNLKQLNIIAAICVAINLSINL
                     LLIPRIMATGSAIASLSAQTFSAIAQVILVVKIFKIRVNKRYLTTLVVFTLVSLIAGF
                     VAKQIGLSWLHGMCLLWVMGLITAMGLNLINVKMLMKAMTEKANNI"
     gene            9545..10678
                     /locus_tag="PLF20_03725"
     CDS             9545..10678
                     /locus_tag="PLF20_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOX80152.1"
                     /translation="MRILVNTRLLLYDKLDGIGWFAFETLKRITAEHPEYEFIFVFDR
                     PFHPDFVFSSNVRAVVAPPPTRHPVLIFLWFELSIPFLIRKFRPDVFLSPDGFLSLSA
                     KVKSLTVIHDLNFEHNPQDLPWKYRVFYRFFFKRYAKKASRIATVSEFSASDISQHYS
                     VIRDKIDVVYNGVAEGFKPLPPERIREIREEFSEGDPYFIFVGSIHPRKNIINLLKAF
                     DLFRLRTSYPMNLLIVGKKQWCSSSIEETHRKMKYQKDVIFTGRLDVDKLQQVVASAF
                     ALTYVSLFEGFGIPLLEAMKCNVPVITSNITSMPEVAGDAALFVDPYRPDEIAQAMEQ
                     LATNEQLRKELIRKGAVRCENFSWELSANLLWQSIMKTIHQTQ"
     gene            10686..11969
                     /gene="purD"
                     /locus_tag="PLF20_03730"
     CDS             10686..11969
                     /gene="purD"
                     /locus_tag="PLF20_03730"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOX80153.1"
                     /translation="MVDVLVMGSGGREHALAWKIAQSSLVKHLYIAPGNPGTSLCGTN
                     VPIDAMDFEGIANFIVENSINLLVVGPEAPLAAGITDFLQSDPRLPHLKIMGPNKLGA
                     LLESSKDFAKKFMIRHHIPTARYKAFRSDDWEEALQFLKSIDPPYVLKADGLAAGKGV
                     VICSTITDATAELEKMLLEKKFGEASQKVLIEEFLDGIELSVFAITDGKHYCMLPEAK
                     DYKRIGEGNTGPNTGGMGAVSPVPFATGDFMQKVEERIVGPTIRGLNEENIPYLGFIF
                     FGLMNVKGEPYVIEYNVRLGDPEAEVVLPRLNSDLVELMMAAVDHRLSDFHLDVEPKN
                     AVVVMLVSGGYPGKYKSHLLIEGLEDISDALVFHAGTMIEKGKYYTASGRVLAITAMG
                     NSLEEAFRNTYRQVNKIFFLDMYFRKDLGKDLLNL"
     gene            11966..12901
                     /locus_tag="PLF20_03735"
     CDS             11966..12901
                     /locus_tag="PLF20_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80154.1"
                     /translation="MINYLENHEINKQMWDACIQKAFNGNIYAYSWYLDVVANGWDAL
                     VEGDYQTVFPLPVRRKFGISYLYQPLFVQQLGIFSQIELTSRIVQNFFEALPSRIKFG
                     RIQLNYHNKFEPSPFIEVTYKPNIELALTQSIEHIRNHYSENTRRNLRKAHHAGLIVI
                     DHQTPDIIVKMFRENRGKHDILWTDNDYKKLIHLFYLLIHKGVGLVRGVFDQNHNMIA
                     GAFFAFSHQRVILLLSAVNEEGRKHGAMHLLIDDTIEKYLNHALVFDFEGSTDINLAR
                     FYRSFGSDTYYYPLIEMNRLPKCIIGFYKKLKRFE"
     gene            12954..13613
                     /gene="upp"
                     /locus_tag="PLF20_03740"
     CDS             12954..13613
                     /gene="upp"
                     /locus_tag="PLF20_03740"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOX80155.1"
                     /translation="MVMVYVLGNQHSVLNQFIYEMRDASIQIDSLRFRTNMERASIIF
                     AYEISKKFRYKKKEVTTVLGTAEVSVPIDTLVITSILRAGLPMHNGMLHVFDHAENAF
                     ISAYRKYDRDGGFHVEVEYVSSPDLTHKILILCDPMLATGSSMVLCYKELLNHGKPLH
                     THFVSLISSVEGIEFLKQQIPPDNYTLWTGAIDDELTAQAYIVPGLGDAGDLAYGKKV
                     K"
     gene            13635..14876
                     /locus_tag="PLF20_03745"
     CDS             13635..14876
                     /locus_tag="PLF20_03745"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX80156.1"
                     /translation="MKLKNAVFYENIRIALASVRSHLVRSFLTISIIAFGIMALVGIL
                     TSIDAIKNFFEENFMHMGANTFTIRNLRIQSESERGGRIGEPIRYNQAISFKERFEYP
                     AVVSVSVNASFSTTAKYKNIKTHPNLRVLGIDENYLITSGEELRVGRSFTSNEVQSGS
                     FVCIIGNDVANKLFKGINDPTGSVITIGPGKYTVVGVLAQSGSSFGFSGDRNIYLPLN
                     NVRQRYSLNNVSYLINIWVSSATELEAAIGETTGFFRQIRNLRLPESNNFDIVRSDSF
                     VNMMLENTRLISLAATIIGLITLCGAAIGLMNIMLVSVTERTREIGIRKAIGANKKTI
                     RNQFLAEAIVIGQLGGLFGIVLGIAIGNLVAIIIGSKFIIPWAWIFLGVFLCFIVGIL
                     SGYFPANRAAELDPIEALRYE"
     gene            complement(15335..15985)
                     /gene="elbB"
                     /locus_tag="PLF20_03750"
     CDS             complement(15335..15985)
                     /gene="elbB"
                     /locus_tag="PLF20_03750"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOX80157.1"
                     /translation="MKRFAIFLSGCGVSDGAEIHEAVMTMLAIDKFDCSYDIFAPDIP
                     QAHVINHLTGEVSNEKRNVLVEAARIARGKIINAAEFEAGKYDALIFPGGFGVAKNFC
                     TYAFDGVKCSVNPIIEKAIKDMHMAGKPIGAMCISPVLITRVLGQVTTTIGTDPSTAH
                     DLEKMGGYHIESSIAEVVSDRVNKIFTTPCYMLNSRISEIALGAENLIKTILNFIQ"
     gene            16095..>16724
                     /locus_tag="PLF20_03755"
     CDS             16095..>16724
                     /locus_tag="PLF20_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80158.1"
                     /translation="MKYKPGDIAEFEVINKKSFPDDREYFVLQDASAMRFLLPAKRYS
                     HYSIECGIYIKCRIDRINCNGRIFLEPFHPYLHKGSSYVFEVYSLFQSNLINIASLKD
                     QLGYDYMVMLRPDYRPSEKILCRVKKISKAKVHLVSEVANPQWLSPPQEGLYTMKNIG
                     IYSHPSGNRFCLAYHPIFGVDLIPLNSVVDAGFEKSPFLQCFVYTDDYEI"
BASE COUNT         4771 a         3152 c         3580 g         5221 t
ORIGIN      
        1 ggaaaaaatt tgtgtttccc gaaagttgaa agttaaagat tcaagttgct gtatacaaag
       61 aaattgcatc cctaaggctc tcaagttggg ttaaattttt gtagttgctt cgaaagtaaa
      121 atcgtggctt ttgtttcctc aagaagagag aattattgtt tatcccgtga tatgagaagg
      181 gagtataggc aaacacaaaa aactgctcct ttgcccgggg cgaaaggagc agttatagaa
      241 caaaggaaca atatccgacg cttagtacat atcacccatt ccaccgggca taccgccgcc
      301 catgggagcg ggtttatctt cttttatttc tgaaactatg cattcggtag taaggagcat
      361 tccggcaata gatgctgcat tttcgagggc gatgcgggct actttagttg gatcgattac
      421 acccgtttgc ataaggtttt catattgttc agtacgggca ttgtaaccga aatcatcttt
      481 tccatcgcga actttctgaa cgacgaccga accttcgagg ccggcatttt cggcgatttg
      541 acgtaaaggt tcttcgaggg cgcggataat gatgttgatt ccaaagtttt cgtcttcatt
      601 ttcgcccttc aggttgcgga tttcttcgat agctctgatg aaagctactc cgccacctgg
      661 tacgatacct tcttcaatag ctgctctagt ggcatgcagt gcgtcatcaa agcggtcttt
      721 gcgctctttc atttcgactt cgctggcagc gcctacttcg attactgcaa cacctcctgc
      781 cagtttggca aggcgttcct gaagtttttc cctgtcgtaa tcactcgtag tggcctttat
      841 ctgtgctttt atttgattga tgcgggcttc gatttgtttc ttttcgccac caccgctaac
      901 aatagtggta ttgtctttat cgattgtaat acgagctgcc tggcccaaat aagacaaatc
      961 ggcatcttcg agtttgtagc ctttttcttc gctaataacc gttccacccg tcaagatggc
     1021 tatgtcttcc agcatttcct ttctgcgatc gccaaaacca ggagctttga cggcaaccac
     1081 cttcaatgtg cccctcaaac ggttgacgac taaagttgcg agagcttcac cttcgaggtc
     1141 ttccgcaatc accagtaatg gacggccggt ttgaatcact ctttccagta cgggcaggaa
     1201 gtctttcatg gcactaatct tcttgtcgta aatcagtatc catggatttt cgtattctac
     1261 ttccattttt tcggtgttgg taacaaaata cggagagatg taaccgcggt caaattgcat
     1321 tccttctact actttgacgc tggtttcgat tcctttggct tcttctatgg taataactcc
     1381 ttctttgccc actttactca tagcttcggc aatgagtttg ccgatgaaaa ggtcgttgtt
     1441 ggccgatacg gcggctactt gttcgatttt ttcgaggctg tcaccaattt gttgggtttg
     1501 gctcttgaga gatttcacta cctcagccac ggctttgtcg atgccgcgtt tgatatccat
     1561 ggggttggaa ccagcggtta catttttcaa accattgaca taaatggatt gtgccaagag
     1621 agtagcagtg gttgttccat ctcctgcatt ttcatttgtt ttggaagcta cttcttttac
     1681 catttgagct cccaaattct cgatcttgtc ttttaattcg atttcttttg ccacggttac
     1741 accgtccttc gtaattgcag gggctccaaa tgacttttca agtaccacat tgcgcccttt
     1801 aggaccaaga gtaattttta ctgcgtcggc cagagcgttt acacctcttt tcagagcctc
     1861 gcgtgcttca aaaccatata cgatttcttt tgctgccata ttctaaatga atttaatgat
     1921 ttaatgtgtt gaattgtatt gttatgagtt ttcatcacag aattgccaga atatccgatt
     1981 cgcgcataat cagataatcc tttccatcga tattgatctc agtacctgcg tacttgccat
     2041 ataaaactac atctcctact tttacggtaa gaggttcgtc ttttttgcca ttaccaacgg
     2101 caattacagt tcctttttgc ggtttttcct tggcagtatc ggggataata attccacccg
     2161 ctgtcttttg ttcagcttct gctggttcta ccagcactct gtcagctaaa ggttttaaag
     2221 tgatatttgc cataatactt gatgttttaa aatttcctgt ggcggcaaag tcaaatatta
     2281 tgccagaggg gaattagcac ggatttatta gatatcatct gaattcgaca aactgaaatt
     2341 ttctcgatag actgtgtttt agtgctttgc gtatatccct gaaataaaaa aaagtgtcag
     2401 aaaaaaaatg acagtctgac actttttcac gtatctattc tatcattgtt actgttcgtc
     2461 aggttgcacg ggttgattga aattttccat tccgggagca ggtcgatagt cgatttgttt
     2521 cttggcagct tcctgctggg cttttaaggc ttcagcagca gtgcttttgg tggttttatt
     2581 ctggacattt ctgggaatta ctgcaacaga gaataaacta agcacgagta atgcaattgc
     2641 aagagtccat gtgcttttct cgagaaaatc ggttgttcga cgtacaccta agaattgatt
     2701 ggaacctgca aaattggagg ccaatccacc tcccttgcta ttttgaacca aaacaaccag
     2761 cgtcaacagg aggcagacta tcaatataag aactgcaaca aatatatatg tactcatagc
     2821 ttattctttt tcgaggtttt gaatttggcc tgcaaaataa gcactttttt cgggatttaa
     2881 caagctcagc tttttataaa tttcaatggc tttttctttt ttcccttgtt gcgcataaat
     2941 ttttgcaagt gtttcactaa cgatatcctc agtaaatgtg ttgctttttt cagagagatc
     3001 gtcgggatta aaaaaactgg ttcgttttcg ttcaatacga ggttcatcac gtataaaacg
     3061 atcgatgaga tccatcttcg attgcaatcc ttcgtttata gttggtgctg tccaattttc
     3121 ttccctactg gaagtttcta aattagatga gagagaattg ttctcaagag gttcattaac
     3181 taattcttca gaagaatttg atgttatttc tgaaattgca gaagtatggg agggtgagcc
     3241 agaggatgat gagtgaaatt tttcatcata agttaatgct tcgcgggcga gtttgtcggc
     3301 ctgtattttg gactgtctga tttgttcgag tttgcgttcg atacttgcca aaagggcttg
     3361 ttttttatct atagcagcat catatgggac gaaagtttcg ataggtcgaa gaggtatgaa
     3421 tggcaggttg gggtttatgt tttccgagaa tgttttggcg ggttggtttt cgcttgattt
     3481 agtttcgcaa gtaaggggta tgaaagtctc gataatactt ataagttttc tcaatactga
     3541 acggtcgccg gcataagcgg ccgaaacttt taaacggtcg aaatagcgga tatcgttttt
     3601 tgctttcaga gaagcaataa gcagcatttg agcggtttgg caataaggat aaagaatggt
     3661 taattccgat agctcatgga tgctggataa actgattttt tcggggttac gtaaaaattc
     3721 tataaattct gtccgggtca tcgaatttac cagttaacca ctgctttatt gaaaatatcg
     3781 tcaaccagag cttcgacaat gagttgaatc aggttatcct ggactgcatt caggttagcc
     3841 gtaaaaggat aatcttcgaa acgggtaaat gtttgttcga agttttgttt ttcatcgaat
     3901 ttattggtga atttaactct cacgctgatg gtcaggcgat taagggcggc ttgttcggta
     3961 ccttgtatgg caacgggttg agtgatataa ctgatgatgc taccttctat atttaaatcg
     4021 ccgtttcgtc gtatgagttg taggggagtt tgagtaatga agcggtttct cagagcttcg
     4081 gtcaatgttt gactgagtga gggcacaacc agtgaagcct gattagggaa ataatcgata
     4141 ctgatagttt ttgcttcgat agggatagag gctcctgtaa atgaataact cattctgcag
     4201 cttgaggata cgagggcgat caaaaacaaa aggatggttt tttttacggg cataaggttt
     4261 acagagagat aattcttaag aatcggtagg tttttccaaa ggaggggtcg agatatccga
     4321 tcgaggggaa ttttttttat tggtataata catatcgagt gaaacgatca tagcatagaa
     4381 ggcccagaat ggcacgctca gtttatcagt atcgaggaaa ttattcatga agccatgaat
     4441 ataataagta atcaagccga tgatgatgcc gatagccagt tgtcggtgat gtcgtgagct
     4501 agctttgtag taaactctga aaccatagta gatagaaaaa gaaaatataa gaacaatgag
     4561 aattgtacct aagagtcctc tttcagcaag tggcccgata tattcgctgt gggcattgcc
     4621 tttatcgcca gcattggtcg aaatgatggt tttttcttta gaaagttgat aaggagcata
     4681 aataaactga taagttcccg gaccccatcc aacgattggc ctttccttgt acaaacgaag
     4741 tgcagcttgc caacgattga tacgttcgag attggatgca tcggttcgga tattggctat
     4801 ggatttaacg tgttctacaa aattggccga agaatcttgt ttgttctttt cgaggcgatc
     4861 gatgatttgc tgctgaaagg cgaagaagag cccgatcacg aggatgagtg tggttaaaat
     4921 ccatttgaac ggaattttta aaagaaccag aataaaaacg ataactccgc ctaaaagtcc
     4981 aatccatgca gcgcggctaa acgaaaggta caatccaatt gaaagcatga tggtggagat
     5041 aatggcaaac cattgccaga agcggccatg gctcttatcg agtgagaggg aaaaacttac
     5101 agggattagt aaagcagtaa tggctccata ggccgtatgg tcattgtaaa aaggatacat
     5161 cacccagtgc cctatttctt cgtcgaaacc caccttggca tggcgaatgg tcgtataaac
     5221 tatcacaatc aataaagaag cgatatatac tcgaatgaat cttcgaatat tcttctcgtt
     5281 cttaaagtat agaattccca taaaatacat gggcacaata aaccacaatt gagagagaag
     5341 gtatttgaat gatactaaag gtaactcgct cgacaaacag gtaatcaaca tccacgccag
     5401 atggataata atgagtatgc tcaaaggatg acggaaaaac gagcggtcaa tgtcgctatc
     5461 ataaatgatc tttatgatga agaaaagcat tagaccaata agtaaaatct ccgaagggac
     5521 agaaatgcta actcccaggt caaaatcttg taaaacaatc gacaaaggag ttaagaaggc
     5581 tactaaaaga ataacgatat cgaatgcaaa aacgaataaa agagcaaggg ctactaagat
     5641 gggaatggca agagcccagt aggtatcgcg tgaaataaga taagtattca gaaggatgaa
     5701 agcggccata atgaggtata accacttcac cttgtatttt tcgatagagc tgattttatg
     5761 aaaagcagct gacataatta ggcattttct ttttttagcc ggctgttctc aataatgcca
     5821 atcactataa ctgataagag cagaacactc aaagtagata cgagaacgat gatccatctt
     5881 actggagttg cctttttatc gggtacctga gggcttgata tgatgttcga ataggtaaat
     5941 tgacggtcgt gatccatgta agctcgatcg taatcttttt taattaaagc ataattggcg
     6001 gcttcctgtc ttatcaattc ctggagcata ataagatctc cacctttttc ttccatgttt
     6061 ttgcgaatac gctgcacttc gttcgtattg attcttcctc ctccgaagtc ggttgttccc
     6121 agataaccct tcaccacttg ctgcgtttga aagctaaagt cgagcaggtt gtaattcacg
     6181 cccaagtcgc gcatgaggtc tttgagggaa tccaactcat gtgattttcg tgtaagatag
     6241 cgttcgtaca tggccaccac ttcgttgaat ttttcgttat gaaggctggc aatcttatga
     6301 ttatagaaat tgataataga attcaccatg tctctggcct gatagggatc aaaatcgtac
     6361 acctcgataa gaacggcttc gtattgtgtt ttgttgattt ttacgttgtg gttgtaattc
     6421 caataaagat tcgatttcca atatttcttg gtggtatcga ttttataatg tttgataaga
     6481 ctgaattttt gaattaagct gtcgcgtata tcggtggatt gcatgatctg caacatctgc
     6541 tcggtgaggc tttcatcgga atattctttg atattggaag gatacaatac ggctgaagat
     6601 ttgtatcggg gtttgatgaa tgaaggactc gaaaagatta ctgagagcac gatggtagcc
     6661 acgactatta tacttaaatg ccatttccat ttaatcagta tttcaatcag tttgaggttc
     6721 ctaaaataat tttccatggt cagcaaatta aaggtaattg gttcaggtat gaagttttta
     6781 agaaaaatga gaaatttatg agatttattg tgcagataag tatcaaaatt tagggggaaa
     6841 gtattattgg gtttgaaatt ttttttttag ctttcaaaga ggagtagttt tcaaggacga
     6901 tgatgatgat tgtcgccagg aataaggatg aaataacagt aagtgtaacg atgagccatc
     6961 ggatgggata ggattttttt tcggccttgt aagctgtagt aaccacgaat ttttgtggca
     7021 ggaattcgaa ggcatctaca cgggcttctt cgtatttggc cttgaggtca ctcaattgtt
     7081 ttttttcgaa ttcgagctga tttcgtatcg atacataagc tcccccataa cgtgcaagga
     7141 catcaagctg ggcttgtagg gcttgaatcc cgcgagtatt tcctctggca atctccacag
     7201 ctaattgctg attgatcatt tcggtttggg tttcatagtc gaacactcct aattctctga
     7261 gtttaaccag cgaatcttcc atttgctgaa tttcggcttg ctttttaaga tattctcctt
     7321 ctacaatatg aaaagccttc agggctcttt cgcgttgcat ggcggttttt gtactgtcga
     7381 acaaggctgc aatatcattg gctatatcag ctgctgtctg agggtcatga tcgagaacgg
     7441 aaacctttat ggccatattt tcagtgcggc gaaaagtaat gttgttttca tattctttat
     7501 aaagcttggt gagcctgtat ttttcgcggg gctttattcc ataatggttg gctaaatcat
     7561 attttgcaat gattctgtct ctaatgagat tcgagttgag tatctgcaac atttgttcgg
     7621 cttgttcttc ggcaccgaat tcgagtacat cctgttgaat tccataagtg ttggataaca
     7681 atgccttcga aatactgctc gtcgaaacag gatacatgat cagggtcgac ttatactttg
     7741 gcttgataaa atatgaggaa gaaaatatga gtgaagcaaa gaatgcaata attgttacaa
     7801 ttagcaatgg tttcccccat atcgaaagga atagaaataa cttttccgaa ctgtagtctt
     7861 ttgtcacttt tgttgtatca tctgtcatat atggccttaa ctaggcaatg ttaaatgagg
     7921 ggtcaaaatt aagaaaaaaa gaatataaat cgataaagaa ttgatgctta tatattattt
     7981 gccttctcgg tcatggcctt catcaacatt ttaacattga tcaaatttaa gcccatggcg
     8041 gttataaggc ccatcaccca taaaaggcac atcccatgta accatgaaag ccctatttgt
     8101 ttagcgacaa aacccgctat aagtgaaacc agtgtaaaaa caaccaatgt ggtgagataa
     8161 cgtttattta cacgtatctt gaatattttg actaccagaa taacttgagc aatcgcagaa
     8221 aaggtttgtg ccgagagact tgcaattgcc gaacccgtag ccatgatacg aggaataagt
     8281 aatagattga tagaaagatt tatagctacg catatggcag ctattatatt cagttgtttt
     8341 agatttccgt ttgctgtgag taaggttcca aaaacgtaag aagacgatat ggaaatgaat
     8401 gcaaacataa gaagagtgaa gatcagggaa gattgattca gacgtgcttg aaattctgcc
     8461 aaactctcgt ccgtatgttg tggatacaac aattgcatta attcgggaga gtaatagagt
     8521 gcagtaaagg ctaacaagat tgaaaagacg aataatgcag aaaatgaaaa ttttataaac
     8581 ggtataatgt ctcctttatg tttgattaaa gtggcaaagg tgggcaaaag aaaaactgag
     8641 aagagataag ctaccatatt gaaagcgtcg agcaaacgat aggcagcggc ataaattcct
     8701 acttgatggt tgccctgggg atcgggaatg agcctttcga gcaacacggg atcgattcga
     8761 ttgtaagtag ccattaaaag agccaggatt gcaaagggaa tgctctgctt gataatcatc
     8821 acaaagaatg gaaaattcca gtgtagcttg gtttttcctg cttttctcca aacaatcatc
     8881 aaagctacga ggcatgtgag caaataagct aacgtttgcg tatatacgaa ccattctatt
     8941 cggaaatgta acctgtaatg ctggttgagt aataaataac ccacaaaaac gatcatcaga
     9001 aatctgtcga gtaccgaaag gatgctgtcg gtgcggaaca tgagcaaacc tgaaatattg
     9061 ctacgcaagt aaaggatcaa tgcaagtaga aattggttga atccaacgac tgccaataaa
     9121 ctcatctgtc ggctgttgta cccaatgatc aaccccgtga gcatgattac tacaaagtag
     9181 agagccgcta aaagaaatct caatacaaga atattggaaa agtgtttggt gagtaaatgt
     9241 cggttttggg caatatttct attattataa ttggttatgc caaaatcgag cagtatgtta
     9301 aaaagaaagg aaaaatttaa aatagtggta taaaacccat actcttcaaa acctacaagg
     9361 ttctgaacac ttctgtcgat tcccaatatc cagaaaggtt tgattaggag attgagaaaa
     9421 agtaaaagcc acaggttgga aataaatttt ttttgcatcc ctgcaaaagt aggaaatacc
     9481 ctttcttctt gtcagaattg tctaaaattg ttaagattcc tatttttgca aaaatgaaaa
     9541 tcctttgcgt attctggtca atacaagatt gcttctgtat gataaactcg atggtattgg
     9601 atggtttgct ttcgaaactc taaaacgtat tactgccgag catcctgagt atgagtttat
     9661 ttttgtgttc gacaggcctt ttcatcccga ttttgtcttc tcatccaatg taagagccgt
     9721 ggttgctccc cctcccacac gccatcctgt tttgatattt ttgtggttcg agctaagcat
     9781 tcctttcctg attcgtaaat tccgtcccga tgtatttctg tcgcccgacg gttttttatc
     9841 gctttcagct aaagtgaaat ctcttacagt gattcatgat ttgaattttg aacataatcc
     9901 tcaggattta ccttggaaat atagagtttt ctatcgtttc ttttttaaac gctatgcaaa
     9961 aaaagccagt cgtattgcta cagtatccga attttcggcc agcgacatca gccagcatta
    10021 ttccgtcatt agagataaga tcgacgtggt atacaatggt gtagccgaag gattcaagcc
    10081 tttgccgcca gaaagaatca gagaaattag agaagagttt tccgaaggag acccctattt
    10141 tattttcgtg ggttctatcc atccgcgaaa aaatatcatt aatcttctga aggccttcga
    10201 tttattcagg ctgcggacat cttacccaat gaatctgttg atcgtaggga agaagcaatg
    10261 gtgtagttct tccatcgaag aaacccatcg aaagatgaaa taccaaaaag atgttatttt
    10321 tacgggaagg ttggatgtgg ataaacttca gcaagtggtt gcttctgcct ttgccttgac
    10381 ttatgtgtct ttgttcgagg gttttggcat tcctttgctc gaggccatga aatgcaatgt
    10441 acctgtgatt acttccaata ttacatcgat gcctgaagta gctggtgatg ctgccttatt
    10501 tgttgatccg tatcgacccg atgaaattgc tcaggccatg gaacaacttg ctactaacga
    10561 acaattgagg aaagaactca tcagaaaggg cgccgtacga tgtgaaaatt tttcatggga
    10621 gctttcggca aatttgttat ggcaaagtat tatgaaaacg attcatcaaa cacaatagag
    10681 aataaatggt cgatgttttg gtaatgggtt caggcggacg tgaacatgcc ctggcatgga
    10741 aaattgcaca aagctccctg gtgaagcatt tgtacattgc tccgggcaat cccggcacca
    10801 gtctctgtgg gactaatgtt cctatcgatg caatggattt cgagggaatt gccaatttta
    10861 tagtggaaaa ttctatcaat ctgcttgtgg ttgggcccga agctccattg gctgccggaa
    10921 ttactgattt tctccagtct gatcctcgct tacctcatct taagataatg gggcccaata
    10981 aattaggagc cttgctcgaa tcatcgaagg attttgccaa aaaattcatg attcgccacc
    11041 atatccctac tgcaagatat aaagcctttc gaagcgatga ttgggaagaa gccttgcaat
    11101 tcttgaaatc catcgatcca ccttatgtat tgaaagcaga tggcctcgct gcggggaagg
    11161 gtgtggttat ttgctccact attaccgatg cgactgctga actcgaaaaa atgttgttgg
    11221 agaaaaaatt tggagaggct tcacaaaaag tattaatcga agaatttttg gatggtatcg
    11281 agttgtcggt atttgcgatt accgatggga agcattattg catgcttccc gaagctaaag
    11341 attacaaacg gataggtgag ggaaatacag ggccaaatac agggggaatg ggggccgttt
    11401 caccggttcc tttcgcaaca ggcgatttta tgcaaaaagt ggaagaacgg attgtcggcc
    11461 caaccattcg aggcttgaac gaagaaaata taccctatct tggatttatt ttctttggtt
    11521 tgatgaatgt gaaaggcgaa ccttatgtca tcgagtataa cgtgagatta ggagatcccg
    11581 aagccgaagt tgttctcccc cgcctcaata gtgaccttgt cgaattgatg atggctgctg
    11641 tcgatcatcg gttgtcggat tttcatcttg atgttgaacc aaagaatgca gttgtggtta
    11701 tgcttgtatc gggcggatat ccaggtaaat ataaaagcca tctcctaata gaaggattag
    11761 aagatatttc ggatgccctt gtttttcatg ccggaaccat gatcgaaaaa ggcaaatatt
    11821 atactgccag tggtcgtgtg ttggctatta cggctatggg aaattctctc gaagaggcct
    11881 ttcgaaatac ctatcgacag gtcaataaaa tattttttct cgatatgtac tttcgcaaag
    11941 atcttggaaa ggaccttctg aatttatgat aaattatctc gagaatcatg agattaacaa
    12001 gcaaatgtgg gatgcctgca ttcaaaaggc attcaatgga aacatctatg cctattcatg
    12061 gtatcttgat gtagtggcca atggatggga tgcactcgtg gagggcgatt atcaaactgt
    12121 gtttcccttg cctgtcagaa gaaaatttgg catctcttat ctttatcaac ctttatttgt
    12181 gcagcagttg gggatttttt cgcagataga gcttacttcc cgaatcgttc aaaatttttt
    12241 cgaggccttg ccctcgcgaa tcaagttcgg tagaatccaa ctcaattatc ataacaaatt
    12301 cgaaccttct cccttcatcg aggtgaccta caaacccaat atagaacttg cactcacaca
    12361 atccatcgag catattagaa accattacag cgagaatacc cgacgcaacc tccgcaaggc
    12421 gcaccatgct ggtttaatcg taattgacca ccaaactccc gatatcatcg taaagatgtt
    12481 tcgtgaaaat cgtggtaaac acgatatttt atggactgac aatgactata agaaacttat
    12541 acatttattc tatttattga tacataaagg tgttggtttg gtgcgtggag tttttgatca
    12601 gaatcacaat atgatagcag gagccttttt tgctttttcg catcaaagag tgatcctttt
    12661 actgtctgcg gttaatgaag aaggccggaa gcatggagcc atgcatcttc tgattgatga
    12721 taccatagaa aaatatttga accatgccct ggtcttcgat ttcgagggaa gtacggatat
    12781 caaccttgca cgtttttaca gaagttttgg ttcagatacc tattattatc cacttatcga
    12841 aatgaatcgc ctgcccaaat gtattattgg gttttataag aagttgaaaa ggtttgaata
    12901 aaaacaaaag tctgcttcgt catgaaaaaa taattacttt taccgcaaac tccatggtta
    12961 tggtctatgt gctgggaaat cagcattcgg tgctgaatca gtttatctac gagatgcgtg
    13021 atgcttccat acagattgat agtctgcgtt ttcgtaccaa tatggaaaga gcttcgataa
    13081 tttttgccta tgaaatcagt aagaaattca ggtacaaaaa gaaagaggtt acaactgttt
    13141 tgggaactgc tgaagtttcg gtgccaatcg atacattggt tataacgagc atactgaggg
    13201 ccggcttgcc aatgcataat ggcatgctgc atgttttcga tcatgccgaa aatgccttta
    13261 tttctgctta tcgcaaatat gatcgtgatg gtgggtttca tgtcgaagtg gagtatgttt
    13321 catcacctga tctgacgcat aagattctta ttctttgcga tcccatgctg gccacaggat
    13381 cgagcatggt attgtgctat aaagaactgc tcaatcatgg caaaccattg cacacccact
    13441 ttgtctctct catttcgagc gtggaaggga ttgaatttct aaagcagcaa attccgcccg
    13501 ataattatac tctatggaca ggtgcaatag atgatgagct tactgctcaa gcctatattg
    13561 tcccaggatt aggcgatgca ggagatctgg cttatggcaa gaaagtaaaa taacccggta
    13621 attttacctg aagcatgaaa ttgaaaaatg ctgtattcta tgaaaatata aggattgcgc
    13681 ttgcttctgt acgcagccat ttggttcgat cgttccttac catcagcatt attgcctttg
    13741 gcatcatggc tttggtcgga atccttactt cgatcgatgc catcaagaat ttttttgagg
    13801 aaaatttcat gcatatgggc gctaatactt ttacaatacg aaatctgaga attcaatcgg
    13861 aaagcgagcg tggaggccgt attggagaac ctattcgcta taatcaggcc ataagtttta
    13921 aagaaaggtt cgagtatcca gccgtggtct ctgtttcagt aaatgcgtcc ttcagcacga
    13981 ctgccaaata taagaacata aaaactcatc ctaatctgag agttttggga atcgacgaga
    14041 attatctcat tacttccggc gaagaactgc gagttggaag aagttttacc agtaatgaag
    14101 tacaatctgg aagttttgtt tgtattattg gaaatgacgt ggctaataaa ctttttaaag
    14161 ggattaacga tcctacaggg agcgtgatta cgataggacc tggcaaatac acggttgtgg
    14221 gggttctagc gcaaagtggt tcgagctttg ggttttcggg cgatcgaaat atttatctcc
    14281 ctttaaataa tgtccgccag cgttattcct taaataatgt ttcttatttg ataaatatat
    14341 gggtttcgag tgctacagaa ttagaggcag ctattggaga aactacaggg tttttccgtc
    14401 aaattcgtaa cttaaggctg ccggaaagca acaatttcga cattgttcgt agcgacagtt
    14461 ttgtcaatat gatgctcgaa aatacacgcc ttatctccct ggccgctacc attattggcc
    14521 ttattacctt atgtggggcc gccatcggat tgatgaatat catgctggtc tccgttaccg
    14581 aacgaactcg cgaaattggc atccgcaaag ctattggagc aaataaaaaa acaatacgca
    14641 atcagttcct cgccgaggca atcgttatcg gacaactggg tggtttgttc ggaatagtgc
    14701 ttggtattgc cattgggaat ctggtggcta taataatcgg aagtaagttc attatcccat
    14761 gggcatggat tttcctgggt gtgttcctgt gctttattgt cggaatttta tcaggatatt
    14821 tcccggctaa tcgtgctgct gaactcgatc ccatagaagc gctgagatat gaatgaatcc
    14881 aaacgtcaat cgaattgccc tattcctatt taattttatt ttctgtgttt cgacttcgat
    14941 cttgaaatac cgaaggataa gaatagatgt tcttttgtta tagtattaat atacaattga
    15001 ttggatattt aataaaaaaa attttcttta actattattt gacgttcccg taatttcact
    15061 ctacgttgaa aaaggctttt acgacgtttg tcaagttaat cctaacgttt gtcctttatt
    15121 aaccacttga caaactgaaa atcattatat tatatttgtg ctgtattatt tagaaaaggc
    15181 acatggctta agtgaaaatt tttggggtta ctttcgaagt aaccttgcta agtgggttat
    15241 tcatttggtt tggtacagtc tgagtcccgg aatttcggga ctcagttttt gacaagacaa
    15301 tggtcgtcga aagtttactg cttggcaaag gcttttattg aatgaaattc aaaatggttt
    15361 tgatgaggtt ttctgctcca agggcaattt cgctaattct cgaattcagc atgtaacaag
    15421 gagtagtaaa tattttattt accctgtcgg aaactacttc cgcaatggaa gattcgatgt
    15481 gataaccacc cattttttcc agatcgtgtg ctgttgaagg gtctgttcct attgtggtag
    15541 ttacctgacc taaaactctg gtaattaaaa ccggactgat acacattgct ccaatgggtt
    15601 tccctgccat gtgcatgtct ttgatggctt tttctattat aggattcaca ctgcatttta
    15661 ctccatcgaa ggcatacgta cagaaatttt ttgccactcc aaagcctcca gggaaaataa
    15721 gagcgtcata ttttcctgct tcgaattctg ctgcattaat aatttttccc cgcgcaatgc
    15781 gtgcagcctc caccagtaca ttgcgtttct cgttggaaac ctctcccgta agatgattaa
    15841 ttacatgggc ctgtggtata tccggtgcaa atatatcgta gctgcagtcg aatttgtcaa
    15901 tcgctagcat cgtcatcact gcttcatgaa tctcagctcc gtctgatacc ccacatccag
    15961 aaagaaaaat tgcaaatctt ttcatacatt tattcattat tggttttgct tcaaagatag
    16021 tgctttttat tgaggttttt ttattcagac agagaaacct tttataaatt tgttccactt
    16081 actctatcct aagcatgaag tataaacccg gcgatattgc tgaatttgag gtaattaata
    16141 aaaaatcatt tcccgatgat agagagtatt tcgttttgca ggacgcctct gcaatgagat
    16201 tcttacttcc tgccaaaagg tatagccatt attccatcga atgtggaata tatataaaat
    16261 gccgtataga cagaatcaat tgcaatggga gaatttttct cgaacccttt catccttatc
    16321 ttcacaaagg ttcttcctat gttttcgagg tttattctct cttccaatcg aacctcatca
    16381 acatagccag tctaaaagat caattgggct acgattacat ggtgatgctt cgccccgatt
    16441 atcgaccttc agagaaaatt ttatgtcgag taaagaaaat ttcgaaggcg aaagtacatc
    16501 ttgtttctga agtggcaaat ccacagtggc tatcccctcc tcaggagggt ttatatacca
    16561 tgaagaatat aggaatctat tcccatcctt ctggaaatcg attttgtttg gcttatcatc
    16621 caatttttgg tgtagattta atccctttaa actcagtagt agatgcaggc tttgagaaat
    16681 cgccattttt acaatgtttc gtatataccg atgattatga aatt
//
LOCUS       DAOORU010000035        16593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4243_length_16593_cov_5.957552, whole genome shotgun sequence.
ACCESSION   DAOORU010000035 DAOORU010000000
VERSION     DAOORU010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16593
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4243_length_16593_cov_5.957552"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="PLF20_03760"
     CDS             complement(<1..1199)
                     /locus_tag="PLF20_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80159.1"
                     /translation="MKKPITLLFVLLLVSGSLWSQLSGTYTVGSGGNYATIAAAIDAL
                     NTSGVVAPGVTFNVLAGHTESATTTLTITATGTSTAPIVFQKSGTGANPLITRMDAGT
                     ISTSSLSADGDAIIKLSGTDYITFNGIDLTANNQGIEYGYYTFKPSGTDGCQYVTIKN
                     STVTMTKGTSAYVIGIYISNGPTSPSAVTGVTVTSTSGINSNIVISGNTITNVHAGIY
                     IRGSSATGFYDSDITIGQLGAENTITNFGGGNVSATYGIYFIYVNNPTVEYNTITSAT
                     HGSTLYGVFYSTVSGIVKGNYNAFTLANNSASSLTYFIYNANTVTSEAFNNNTFAAGT
                     LSSTGTVYLIYASNGTPEVNINNNSISGSINRTGASGSFYCYYNLGSPTSGSENIQNN
                     NFSNITVT"
     gene            complement(1680..3839)
                     /locus_tag="PLF20_03765"
     CDS             complement(1680..3839)
                     /locus_tag="PLF20_03765"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HOX80160.1"
                     /translation="MIFIKKLYLTLIACCVFCTGLYADEGMWLPYLLDQLQIVRMQSM
                     GLKLSAEDIYSINHSSLKDAIVIFGRGCTGEVVSADGLVFTNHHCGFGAIQSSSSVEN
                     DYLKEGFWARSYKEEIPISGLTVQFLMFVQDVTHDMLEGVDEEMPETQRDSVLKINEK
                     HILEAIPAQDGLGGVVKEFYAGNQFYLFVYQSYKDIRLVGAPPTAIGKFGGDTDNWMW
                     PRHTGDFSVFRIYTSPSGKPTTYQVNNIPLKTNRFLTINAGGIKEGDFTMIMGYPGRT
                     DRYITSWGVDQALNITNPSIVKIRDKKLEIIRNSMATSDEIRIKYAAKYASISNYWKY
                     YIGQNKGLKRLKIYEKKKQQEKEFAEWIAADTYRFNRYGEVLDSFEQAYRLLNQTAKP
                     SVYIREAAFGPEIIAFASRFASLASTEMKDSKDINAANLHTNAKGMGDSFFKNFDLAT
                     DKKLFAAMMNMYYNDIDPGFIPNILSNANKKYKGQWDKYADEVYEKSMFSSMEAFSKF
                     LLNPSASSLKDDPVYQLALAFREISRKIEAMNIQAKDQLKRSKRLYMEGIQQMGAGNP
                     FYPDANQTMRLTYGQVLDYQAADAIEYSYFTTHFGILEKEEPSNPDFQIPQRLKEVFN
                     RKDFSPYYKNDTVVACFLTNNDITGGNSGSPVLNGKGELIGLAFDGNWEAMSGDIAYE
                     PELQRTINVDIRYVLFLIDKYARSNHIMRELKIEYER"
     gene            complement(3855..4949)
                     /locus_tag="PLF20_03770"
     CDS             complement(3855..4949)
                     /locus_tag="PLF20_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOX80161.1"
                     /translation="MNPNDYCVIMAGGIGTRFWPISRADHPKQFIDILGTGQTLIQQT
                     FQRFKPLCLTQNIFVVTNEEYFSMVREQLPELPPENILCEPSGRNTAPCIAFASYHIA
                     SINPSANIVVAPSDHIIQDMQRFHEVIGTALRTVREHNWLLTLGIQPSRPDTGYGYIQ
                     FVDEIIPGVDSRIKKVKTFTEKPNLELARAFLASGDFLWNAGIFIWNLQTILQALEAY
                     LPEVASLFNEGKEIFNTPEEKAFIEHVYTHCKSISIDYGVMEKADNVYTLMSDFGWSD
                     LGTWASLYEVRQKDSDGNSIVGNQVMSYDTHNCIIHVPDEKLVVIQGLNNCIVVEDEG
                     MLLICNKNDEQQIRSIVNDLKSRKYINLKR"
     gene            complement(5004..5633)
                     /locus_tag="PLF20_03775"
     CDS             complement(5004..5633)
                     /locus_tag="PLF20_03775"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HOX80162.1"
                     /translation="MTIRERSSIEKNLAPLLPEAALHQVVDLLFRFQVDLVVTYPRSG
                     RLGDYLYNTKNNRHRISININLNRYQFLITLLHEFAHLLVQERFKTEVRPHGKEWHAA
                     FCEISMPFLRDDIFPLDVKAAFGAHLKSRYGSAYADKKLSKILERYNGIEQNRYKVEL
                     GTLPVSSKFFLSGRSFQCISRQGNVILCQELKTGTIYRMSSSVFVKPYL"
     gene            complement(5906..6736)
                     /locus_tag="PLF20_03780"
     CDS             complement(5906..6736)
                     /locus_tag="PLF20_03780"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOX80163.1"
                     /translation="MCGRYILVQKLENIEKRFNVTAAFDTLLKPQYNISAGAWVPIIG
                     SDDPHHLQLMRFGITPFWSKTDKFFINARAEGDHNSENLPHYKGAKGIIEKPAFRRPI
                     RSQRCLVIADAFIEGTIQEKLNKPFVVYLRNKVRPFAMAGIWDEWKNPETGEIVRSFA
                     IITTVANELLLKLPHSRMPVILTPAEERKWLDLSLPLSDMTAMLEPYDASLMNAYPIS
                     PVIKSPKAEGAKLISPIGPPVVPEDDLQVKSYLQRSGMGNPKQNFSDHLPEDEISSSD
                     "
     gene            6839..7387
                     /locus_tag="PLF20_03785"
     CDS             6839..7387
                     /locus_tag="PLF20_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOX80164.1"
                     /translation="MMSFLDLVKARHSTRNFENRPVEREKLECCLEAARLAPSASNSQ
                     PWTFIVVDDPGLKEEIARETYSPLVSFNKFVLKAGAIVVIVIEKPKMITQIGESIKKK
                     EFPLIDIGIAAEHFCLQAQEEGLGTCILGWFNEAPIRSLLRIPQSKTIGLLVAVGYPG
                     NETLKEKKRKPMEEFRKYNSYH"
     gene            complement(7391..8341)
                     /locus_tag="PLF20_03790"
     CDS             complement(7391..8341)
                     /locus_tag="PLF20_03790"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOX80165.1"
                     /translation="MEIYRYSDHLPAFNKPVVTVGTFDGVHLGHRRIIDRLKEIARQI
                     QGETIVITFEPHPRLVLAQYGTPVKLINSTYRKYQLLSDLGIDHLIILPFSVEFSMTP
                     WEVFIREYIINQIHTSYLVVGYDHRFGHDRQGDRSKLQAFAHELGLNIEEVPPLFIEG
                     QVVSSTRIRNALHAGNIRLANLLLGYEYSISGKVIRGNRLGHRLGFPTANILADDSLA
                     LIAANGVYVSRVEYKGKIYGGMSNIGIRPTIAHHSFAIEVHIFDFNEEIYDEMINISF
                     IDRLRDEIRFDSLDQLKDQLQEDRILARKILQEVGYGVDL"
     gene            complement(8345..9343)
                     /locus_tag="PLF20_03795"
     CDS             complement(8345..9343)
                     /locus_tag="PLF20_03795"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOX80166.1"
                     /translation="MKIAIIGATGLVGRKMVEKLGSSFISRDAEVFLAASEKSYGKEF
                     FLQGKTLKVSSIENVLEQKPHIALFSAGNWVSEKYAREFVRQGAFVIDNSSYWRMHDD
                     VPLVVPEINSENITSKTRLIANPNCSTIQMVLVLSGLHKAWCIRRIVVATYQSVTGTG
                     VRALNQLMNERTGMIVSEPAYPHTIDLNLFPHGGNFLPNGYTTEEMKLVNETRKILHD
                     NQLQVTATVVRVPVKGGHSEAVNVEFVEDYDIDDALEILRNTPGVVVQDSPSEDIYPM
                     PLTAEGKDEVFVGRIRHDESCRKALNLWIVADNLLKGAATNAVQIAEYIVNRKYIQ"
     gene            9488..12910
                     /locus_tag="PLF20_03800"
     CDS             9488..12910
                     /locus_tag="PLF20_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOX80167.1"
                     /translation="MKIINRAHKKVSMLRKLMLVTFCLGSIQILEAQFTDDFSDGDFS
                     VNPSWTGTSNSFIVNSAKQLQLNAPVEGIAWLSTASSSVEATEWRFWIKLAFSPSANN
                     FARVYLISDEEDLTGPLNGYYLQFGETGSNDAIELFKQQGTNTTSICRGTNGSIASAF
                     AIRIKVVHKPDGEWLIYSDPAGNENFQLQAIGSDVSITAGNRFGIFSKFTSSNITKMY
                     FDDFYTGPIIIDTIPPVLQLLTITSDSSLLLKFSEPLEPSSAQNTTNYLLSPGDIHPF
                     QANFLPNDASSVQLIFSHIFQSGVNYTLSINNLTDLSGNNTGMIQHHFCLYSVKPYDI
                     VINEIMADPEPSVGMPLWEYIELFNTTSVEISLKDWKLKIGNTLKILPEVSMGPQSYL
                     ILCRPEAQTELSAYGITAPITSFLLNNTGQDLQLLDKSNVLVSKVSYTDKWYRDVTKS
                     NGGWSLEQIDPLSPCLGSKNWIASINISGGTPGGINSVYSTNQLFPLPEMVIPIPPHQ
                     LNVVFNQSMDSLSLTNLANYSLLPKFGNPSSIELVDSDFTTLILYYSESFDLSGSYRL
                     VFSTQLFNCIGQAIPENIYWDFNIPSPSKIFDVLITEVMADPDPPIHLPNAEYVEIYN
                     CCDYTVDLSGWSFSIENTDHVFDRYYLKANEYLILAHEKYASDFATYGNFYGFSSFSL
                     PNTGQSLVLKNGKGEAIHWVDYDQNMYGKSPKKDGGWSLEMIDTENPCTIESNWIPSI
                     DPHGGTPGRKNSVSASLPDTISPRFSRIYPLSPKKIWLYFSEPVKQFEATNPENYVLS
                     DGLTFLKIQAIDHRNKVFEAELSSELKPAIIYTVSAKNGITDCAGNCLSNNKEIIFGL
                     PLRPARGKLVINEILTNPLEGGSDYLEVYNSSDTLSDLGELALSYISSSGSSTTIFLP
                     PFLLLPSEYYCLTRNPLSVINQYPLHQKDNFVQVKNFQDLSSSGGTLILYLKEDSTKV
                     IDRFSYSSSMHLPILKNFDGVSLERINPLRPTDDPTNWTSASEASGFGTPAHENSQYS
                     SLPVAKGSLTIEPEIFSPDGDGIDDIVNITIQPPAEGYITNVTLFDSRGRLVKHLVKN
                     LIVGNKCTISWNGMRYDNSRAPVGFYIIYCELFDTNGSTEKHKATVVVGAKL"
     gene            complement(13513..14997)
                     /locus_tag="PLF20_03805"
     CDS             complement(13513..14997)
                     /locus_tag="PLF20_03805"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOX80168.1"
                     /translation="MGKVKQLAGQTAVYGLSSILGRILNYLLVPLYTRVFLPHESAVY
                     VELYAYMAILAVILTYGMETAFFRYSETHSKPKMVYSTAFNSIVITTILFLLFTLTFR
                     DSIASWLGYEGKTEYIIYFLLILGFDALSAIPFAWLRAQQKAWTFAGIKLANIFINIA
                     LNLILILLLPYLYNKYGWSWLGIFTGGKPEVSAIFISNMVASFATLLLLWPAIRKASL
                     TIDLGLWKEMFLYAFPLLVYNLAGVVNETIDRILLKHFLPPETAIHDLGVYGMCFKFS
                     VFIVIFIQAFRYAAEPFFFKQAKEKDAPETYAMVMNYFIIVCFGMFAGIMLFIDVIKY
                     FVGPMYYAGLPIVPLLLMGHVFLGIYYNLSVWYKITGKTMYGALFSLIGTAVTIGLNI
                     FLIPRIGFMGSAWANFSCYLLMMLLSFIYGQKHYPIPYRLDKFFLYLTLSIALFLIGS
                     KIHLPWLGLDLVIKGILFVLFFIIVLRKEPELRQALILAVPKRR"
     gene            complement(15125..15484)
                     /locus_tag="PLF20_03810"
     CDS             complement(15125..15484)
                     /locus_tag="PLF20_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX80169.1"
                     /translation="MNNFLSSARDFSYSGGGIVVKDGKNKENILSSLTDSGIGIYEEN
                     LENLSRIDFTRSSAGTHGEEPMGLGLIFLKNSWVIVKLRFWSKARKIRALPWRFTFRP
                     CDSMKNITRNERFILPI"
     gene            15696..16136
                     /locus_tag="PLF20_03815"
     CDS             15696..16136
                     /locus_tag="PLF20_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOX80170.1"
                     /translation="MRTVSGLDGHKDSVFVCFLDEQDKKILEKHYGTLTPDLTTLRDA
                     LIEHDCGSIAMESTSIYWMPVWHVLESDFSLKLVNPYFIKQLPGRKSDVKDAQWIATV
                     LHKGLLRGSLIPCKEIKKLLKYNPVTWVALFRSGNTMHHLLNGL"
     gene            16349..>16593
                     /locus_tag="PLF20_03820"
     CDS             16349..>16593
                     /locus_tag="PLF20_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80171.1"
                     /translation="MVSISALLTAWAVAAGTAWPFNIPAAFKIALCLSWFSRYMIKSI
                     TSRLLPWVLQLEIKQYAVWVLVVNLHARGLIFVEEAA"
BASE COUNT         5091 a         3481 c         3169 g         4852 t
ORIGIN      
        1 gttacggtga tattgctgaa attattgttt tgtatattct ctgaaccaga agtgggagat
       61 cctaagttgt aataacaata aaacgaacca cttgcaccag tccgattgat gctcccactt
      121 atggaattgt tattgatatt cacttcggga gtaccattac tggcgtaaat gagatacaca
      181 gtccccgtag aagaaagcgt tccggcagca aaggtattat tgttgaaggc ttcactggta
      241 actgtatttg cattataaat aaaataagtt aacgatgaag ccgaattatt tgccaaggta
      301 aaggcattat aattcccttt gactattcca cttactgtag aataaaatac cccataaagg
      361 gttgatccat gcgtggcact ggttatcgtg ttgtattcaa ccgtcggatt gtttacataa
      421 ataaagtaaa taccataagt tgccgaaaca ttcccacctc caaaattggt aatggtattc
      481 tccgcaccca attgcccaat ggtaatgtcc gaatcgtaaa aaccagtagc acttgaacct
      541 ctaatgtaaa ttcccgcatg tacattggta atggtattcc cacttataac aatattggaa
      601 ttaatacctg aggtggaagt aaccgtaacc cctgttactg ctgaaggaga ggttggccca
      661 ttgcttatat aaatgccaat gacataagca ctcgttccct tggtcatcgt aactgtagaa
      721 ttcttaatgg ttacatattg acacccatcc gtccctgaag gcttgaatgt gtaataacca
      781 tactctattc cctgattatt agccgtaagg tcaattccat taaaagtgat atagtcggta
      841 ccgcttaatt tgatgatggc atcaccatca gcacttaaac ttgaagtaga tattgtacca
      901 gcatccatcc gtgtaattaa aggattagcc cctgtaccgc tcttttgaaa cacaataggc
      961 gcagtagacg ttcctgtcgc agtaatcgta agcgtagttg tcgcactttc tgtgtgtcca
     1021 gccagtacat tgaatgtaac cccaggagca acaactcctg aagtattgag tgcatcaatt
     1081 gcagccgcaa tagtggcata gttgcctcct gaacctacag tataagttcc tgataactga
     1141 ctccagagag aaccagatac caacagcaac acaaaaagaa gagtaattgg ttttttcata
     1201 agaggttgat tttaaaatga ttatggaatg gaatgctatt aagttaaagg gaaagaaaaa
     1261 gtaaattatt ggatagttat actaaaaaat tggcgataaa agtaagccat taaattggat
     1321 ttgtcaatag ttttagaaaa aaatttataa aaaaacaaga ttttgaagat gaaatatcag
     1381 aacattggaa aataataaat taacgaaagt tgcttacagg atatcaaata atagcagaca
     1441 ataagcacat ccacaccagg catccctctc ggcgtccgaa ataagttagt cattattgcc
     1501 ggctttcctc ttttaatcac catccatttt taatcaccat ccatcttgca ctatatatca
     1561 cctgatcaca gaactcttat cccgccatac ttttttcttt tcgggaatca ccattccccc
     1621 atctcacaaa ttagcaaaga tgataaaaaa atcacaccat tgtaatttat cgacttggct
     1681 taccgctcgt actcaatttt cagctcacgc attatatgat tactccgggc atatttatcg
     1741 ataagaaaca atacgtagcg aatatccaca ttaatggtac gttgaagttc aggttcataa
     1801 gcaatatcac cgctcatagc ctcccaattc ccatcgaatg ccagcccaat taattctcct
     1861 ttcccattta aaactggact cccactgttt ccacccgtaa tatcattatt ggtaagaaaa
     1921 caagcaacaa cagtatcgtt tttataataa ggcgaaaagt ccttacggtt aaaaacctct
     1981 ttcaatcgtt gaggaatttg aaaatcagga ttcgaaggtt cttctttttc caaaatcccg
     2041 aaatgtgtcg taaaataact atactcgata gcatcggccg cctgataatc caatacctga
     2101 ccataggtaa gacgcatggt ttgattggca tcgggataga acggattacc agctcccatt
     2161 tgctgaatgc cttccatata tagccgtttg cttcttttta attgatcttt agcctgaatg
     2221 ttcattgctt cgattttcct gctgatttct ctaaaagcca gggcaagctg atagacagga
     2281 tcatccttaa gggatgaagc tgagggattt aacagaaatt tcgaaaaagc ttccatactc
     2341 gaaaacatcg atttttcata cacttcatct gcatatttgt cccactggcc tttatatttt
     2401 ttattggcat tcgaaagaat gttcggaatg aaaccagggt ctatgtcatt ataatacata
     2461 ttcatcatag cagcaaaaag tttcttatcc gttgcaaggt cgaagttttt gaagaaagaa
     2521 tcacccattc ccttggcgtt agtatgaaga ttggctgcgt taatgtcttt actatctttc
     2581 atctcagtag aagcaaggga tgcgaatcga gaagcaaagg caatgatttc agggccaaag
     2641 gcagcctctc tgatgtaaac gctaggtttt gccgtttggt tgagtagtct gtaggcctgt
     2701 tcaaagctat cgagaacttc accatatctg ttaaatcggt aagtatcggc cgctatccac
     2761 tcggcaaatt ccttttcctg ttgttttttc ttttcataga tttttaatcg tttcagaccc
     2821 ttgttttggc cgatgtaata tttccaataa ttagaaatgc tagcgtattt ggcagcatat
     2881 tttattctga tttcatcgct cgttgccatg ctgtttcgaa tgatttcgag tttcttgtcg
     2941 cgtattttta ctatggaagg atttgtgata ttcaaagcct gatccactcc ccaagaagta
     3001 atgtatcggt cggtacgacc tggatagccc ataatcatgg taaaatctcc ttctttaatc
     3061 cctccggcat taatagtaag gaatcgattg gttttcagag gaatattatt tacctgatag
     3121 gtagtaggtt ttccgcttgg gctagtataa attctaaaga cggaaaaatc gcccgtatgc
     3181 cgaggccaca tccagttatc ggtatctcct ccaaattttc ctattgcagt aggaggagct
     3241 cccaccaggc gaatatcttt ataagattga tatacaaaaa gataaaactg attcccggca
     3301 taaaactctt tcacaactcc acccaacccg tcctgagccg gaatagcctc gagaatatgt
     3361 ttctcattga ttttcaaaac ggaatcacgc tgagtttcgg gcatttcttc atcaactcct
     3421 tcgagcatat catgtgtaac atcctgaaca aacataagaa attggaccgt gagaccagat
     3481 attgggattt cttccttgta acttcttgcc cagaaacctt ccttcaaata atcgttttca
     3541 acggaactcg aactctgaat cgccccaaaa ccacaatgat gattggtaaa aaccaaacca
     3601 tctgccgata cgacttcgcc cgtgcatcca cggccaaaaa ttactatggc atccttcagg
     3661 ctcgaatggt taatgctgta aatatcttcg gccgaaagct taagccccat ggattgcatg
     3721 cgtacaatct gaagctgatc gagtaaataa ggaagccaca ttccttcatc agcataaagg
     3781 ccagtacaaa aaacacaaca agctatcagg gttaaataca gttttttaat aaaaatcata
     3841 ttcaagatct ttgattaccg tttaagatta atatattttc tcgattttaa atcattcact
     3901 atgctacgga tctgctgttc atcattctta ttgcaaatca gcaacattcc ttcgtcttcc
     3961 accacaatac aattgttcaa tccctgtatt actacaagct tttcatctgg tacatgaatg
     4021 atgcaattat gtgtatcgta gctcataact tgatttccaa cgatggagtt tccatctgaa
     4081 tctttctgac gtacttcata caatgaagcc caggttccca aatcagacca gccgaaatcc
     4141 gacatcagcg tataaacgtt atcggctttt tccattacgc cataatcaat ggagatgctt
     4201 ttgcaatggg tataaacatg ttcgatgaat gctttttctt cgggggtatt gaaaatctct
     4261 ttcccctcgt tgaataggga ggcaacttcg ggcagataag cttcgagggc ttggagaatc
     4321 gtctgcaggt tccagataaa gatgcctgca ttccacagaa aatcaccgct ggcaaggaaa
     4381 gctcttgcaa gttcaagatt gggtttttcg gtaaaggttt taactttctt gatacgagaa
     4441 tctactccgg gaataatttc atcaacaaat tgaatataac catagcccgt atcgggcctg
     4501 cttggctgaa tacccagcgt aagaagccag ttgtgttctc ttacagtcct taatgcagtt
     4561 ccaatgactt cgtgaaacct ttgcatatct tgaataatat gatcggaagg agcaaccaca
     4621 atattagccg agggattgat acttgcaata tgataactgg caaaagcaat acatggagcg
     4681 gtattgcgtc ccgatggttc gcacaaaata ttctcgggag gtagttctgg aagttgttcc
     4741 cttaccattg aaaaatattc ttcgttcgtg accacaaaaa tattttgcgt taagcataag
     4801 ggcttaaacc tttggaaagt ttgctggata agtgtttgcc cagttccaag aatatctatg
     4861 aattgcttgg gatgatcggc acgactaatg ggccagaaac gcgttccaat gccacctgcc
     4921 ataattacac aataatcatt tggattcatt tcgcaataat tttatttttt ggctgtttgt
     4981 gcaaatgtaa ccatttcatt tttctacaaa taaggtttta caaaaacgga agaactcatc
     5041 ctataaatcg ttcctgtttt aagttcttga caaagtataa cattgccctg acggcttata
     5101 cattgaaacg acctcccaga gagaaaaaat ttgcttgaaa ccggcaaagt acccagttcg
     5161 accttatagc gattttgttc gattccatta tacctttcaa gaattttact caatttttta
     5221 tcggcataag cggaaccata acggctcttc aaatgtgcac cgaaagctgc cttgacatct
     5281 aaggggaaaa tatcatcacg aagaaaaggc atcgaaattt cgcagaaagc agcatgccat
     5341 tccttcccgt gaggtcttac ctctgttttg aatctttcct gaaccagcaa atgggcaaat
     5401 tcatgtaaaa gagtgattaa aaactgatag cggttgagat tgatatttat gctgatgcga
     5461 tgcctgttat tctttgtatt gtataaataa tcgcccaatc ttccactgcg gggatatgtt
     5521 accaccaaat cgacctgaaa acggaacaat agatcaacaa cctgatgaag ggcagcttca
     5581 ggcaataaag gcgccagatt tttctcgatt gaactccgtt cccttatcgt cataaatcat
     5641 tgggtttaca actctcactt gagtaaacga cgggcagtcg caatctcctg atttcttttt
     5701 tgtctttctg tgctgggcct tgctcgaagt ctggcaagaa acaacccata cgaaaagaaa
     5761 aatcatacct aaaaacctta gccctaatac tattgtatgt gttttcaaat aatttcgcaa
     5821 cggtttcttc atgaaaaata tttcctttat agtaaatttc catttaatta accacaaacg
     5881 ccgaaaatta ttatgatcgc aagaattaat cgctggatga aatttcatct tctggcaggt
     5941 ggtcagaaaa attctgtttg gggtttccca tacctgagcg ctggagataa gatttcacct
     6001 gcaggtcatc ttcgggtacg acaggcggcc caattgggga aatcagtttg gcgccttcag
     6061 ccttgggaga tttaataacc ggacttatag gataagcatt catcaaagaa gcgtcatagg
     6121 gttcaagcat ggcagtcatg tcggaaagtg gcaagctaag atcgagccat ttcctttctt
     6181 cagcaggggt tagaataacc ggcatgcgac tatggggaag cttcaataaa agttcgttag
     6241 ctacagtggt aatgatggca aaagaacgca caatttctcc agtttccgga tttttccact
     6301 cgtcccaaat acctgccata gcaaaaggtc gaactttatt ccggagatat actacgaatg
     6361 gcttattcaa cttttcctgg atagttcctt cgataaaagc atcggcaatc accaagcagc
     6421 gttgtgagcg gataggcctg cgaaaggcag gtttttctat aattcctttc gcacccttgt
     6481 aatgtggtaa attttcgcta ttgtgatctc cttcggctct ggcattgatg aaaaatttat
     6541 cggtcttcga ccagaacggc gttataccaa aacgcatcaa ttgcaaatga tgaggatcat
     6601 cgctgccgat aatgggaacc caagcacctg cagaaatatt gtattgaggc tttaaaagag
     6661 tatcgaatgc agcggttaca ttgaaccttt tttcgatatt ttcgagtttc tgcaccaaaa
     6721 tataccttcc acacattctt tccgaagaat tgaatttgaa aaagtaaaat taaaccaaaa
     6781 gccaaaagtt tatgattaaa tttgtatttt tattcgataa tcattaaaaa ttaaaaacat
     6841 gatgagtttt ttggatcttg taaaggcgag acatagcact cggaatttcg agaatcgccc
     6901 tgtggaacgt gaaaagctcg aatgttgcct cgaagctgca aggctcgctc cttcggcaag
     6961 taattcgcag ccctggacct ttattgttgt ggatgatcct ggtttgaagg aagagattgc
     7021 aagagaaact tatagccctc tggtatcatt caataaattc gtcttgaagg cgggagctat
     7081 tgtggtgatt gtaatcgaaa agccgaaaat gataactcag ataggtgaat ctattaaaaa
     7141 gaaagaattt ccgctgatcg atataggaat tgcagccgaa catttttgct tgcaagcaca
     7201 ggaagaagga ttaggaactt gcattttagg atggttcaac gaagctccca taagaagtct
     7261 tttacgcata cctcaatcga aaaccattgg acttcttgta gctgtgggtt acccagggaa
     7321 tgaaactctt aaagaaaaga agagaaagcc tatggaggag ttcagaaaat acaattcgta
     7381 ccactaaatt ctaaagatca acgccatatc caacctcttg cagtattttt cgggccagta
     7441 ttctatcttc ctgcaattga tctttgagtt gatcaagaga atcgaacctg atttcatccc
     7501 ttagcctgtc aatgaaacta atattgatca tttcatcata gatttcttca ttgaaatcaa
     7561 aaatatggac ttcgatggca aaggagtggt gggcaatggt aggtcgaatt ccgatattgc
     7621 tcatacctcc ataaatttta cctttatatt cgactcgcga aacataaact ccattggccg
     7681 caatcagagc caagctatcg tcggccaaaa tattggccgt aggaaaaccg agccgatggc
     7741 ccaggcgatt tccacgaatc acttttcctg aaatggaata ttcatatccc aagagtaaat
     7801 tagccagccg aatattcccg gcgtggagag cgtttcgtat tcgtgtagag ctcactacct
     7861 ggccttctat aaataaagga ggaacttctt cgatattcaa acccagttcg tgggcaaaag
     7921 cctgaagttt cgaacgatcg ccttgccggt catgcccgaa acggtgatca taaccaacta
     7981 cgagatagga tgtgtgaatc tggtttataa tatattcgcg tataaaaacc tcccaaggag
     8041 tcatcgaaaa ttccacgcta aagggaagaa taatcaaatg atctataccc aagtcggata
     8101 aaagctggta tttccgataa gtggaattga tcaattttac cggggttcca tattgggcca
     8161 atacaagcct cggatgaggc tcgaaggtaa ttactatagt ttcaccctgt atttgccggg
     8221 caatttcttt caaacgatcg atgatccgac ggtggccaag atgaactcca tcaaaagtac
     8281 ccacagtaac taccggttta ttgaaagcgg gtaaatgatc agaataccta tatatttcca
     8341 attcttattg aatatatttt cgattgacaa tgtattcggc tatctgaact gcattagtgg
     8401 cggctccttt caataagtta tcggctacaa tccatagatt gagtgctttt cgacaagatt
     8461 catcatggcg tatgcgccct acaaaaactt catcttttcc ttcagcggtc aatggcatgg
     8521 gataaatgtc ttcagaagga ctatcttgaa ccacaacgcc tggcgtattt ctcaaaatct
     8581 cgagagcatc atcaatatcg taatcctcaa caaattcgac attcacggct tcgctgtgcc
     8641 ctccctttac aggcaccctc accaccgtag ccgtaacctg tagctggtta tcgtgtaaga
     8701 tttttcgggt ttcgttcacc agtttcattt cctcggtagt atatccattg ggcagaaaat
     8761 tacctccgtg tggaaaaaga ttgagatcga tggtatgagg ataagcaggt tcggaaacaa
     8821 tcatcccggt acgttcattc atcaactggt tcaaagccct aacaccagtg cccgtaacag
     8881 actgataggt agctaccaca atcctgcgaa tacaccacgc tttatgaagc cctgataaga
     8941 ccaaaaccat ttgtatggtc gagcaattgg gatttgctat caagcgggtt ttggaggtaa
     9001 tgttttcgga attgatttcg ggtacaacca atggaacgtc atcatgcatc cgccaataac
     9061 tgctgttatc aatcacaaat gcaccctgcc tgacaaattc tctggcatac ttctccgata
     9121 cccagtttcc agccgaaaac aaggcaatat gcggtttttg ttctaaaaca ttttctatgc
     9181 tgctaacttt caaagttttc ccctgcagaa aaaattcttt cccgtacgac ttttcactcg
     9241 cggctaaaaa tacctcagca tcacgagaaa taaatgaaga cccgagtttt tcaaccattt
     9301 tccggcctac aagcccggta gctcctataa ttgctatctt catgagttgg ttctgtctat
     9361 tgaaaatata ttccttccga agagagatta ttatttattt tgtgaaagcc aaatgataca
     9421 aatgggcaaa attactatat ttaccgatgt aaagagaacc caagatttat caaacttttc
     9481 tcaagcaatg aaaataataa acagggcgca taagaaagta agcatgctaa gaaaactcat
     9541 gctggtaacg ttttgtcttg gatccatcca aatattggaa gcacaattta ccgatgattt
     9601 ctccgacggg gacttttcag taaatccatc atggacagga acttctaatt cttttattgt
     9661 aaattccgcc aaacagttgc agctgaatgc acctgtggaa ggtattgcct ggctctcgac
     9721 tgcttcttcg agcgtagaag ctaccgaatg gagattttgg atcaaactgg catttagtcc
     9781 ttctgcaaat aacttcgcca gagtttatct gatttccgat gaggaagacc ttactggacc
     9841 cttgaatggt tattatttgc agtttggaga aacaggttcg aatgatgcta tcgaactctt
     9901 caaacaacaa ggcacaaata ctacctccat ttgtagggga accaatggta gcatagcatc
     9961 ggcctttgct atccgcataa aagtagttca caagcccgat ggtgaatggt taatatattc
    10021 tgaccccgca ggaaatgaaa actttcagtt acaggctatt ggctcagatg ttagtatcac
    10081 tgcaggaaac aggtttggaa tattctcaaa atttacctcg agcaatatca ccaaaatgta
    10141 tttcgacgat ttttatactg gaccaatcat catagatacc attcctcctg ttttgcagtt
    10201 gttaacaatc acttccgaca gcagccttct gctcaagttt tctgaaccct tagaaccatc
    10261 atcggctcaa aacacgacga attatttgct ttctcccggc gatattcatc cgttccaggc
    10321 aaattttcta cctaacgatg cgagttcagt tcaacttata ttttcacata tttttcagtc
    10381 aggcgtgaat tatactctat ctatcaataa tttaacagat ttgtcgggaa acaatacggg
    10441 catgattcaa caccattttt gcttgtattc ggtaaagcct tacgacatcg tcatcaacga
    10501 aattatggca gaccccgaac cttcggtagg aatgcctctg tgggaataca tcgaattatt
    10561 caataccact tcggttgaaa tctctcttaa agattggaaa ttaaaaattg gtaatacctt
    10621 gaaaatactt cccgaggtta gtatgggccc tcaatcttat ttgatattgt gccggccaga
    10681 ggctcaaact gaactatcgg cctatggtat caccgcaccc attacttcat ttttactcaa
    10741 taatacgggg caagacctgc agcttttaga caaaagcaat gttttggtat cgaaagtaag
    10801 ttataccgac aaatggtatc gcgatgtcac caaaagcaat ggcggatggt cgctcgaaca
    10861 gatcgatcct ttatccccgt gtctgggaag taaaaactgg atagcatcaa taaatatttc
    10921 aggaggaaca ccaggcggga tcaattcggt ttattccacc aaccagttat tccctttgcc
    10981 cgaaatggtt atacctatcc ctccccatca gcttaatgtg gttttcaacc agagcatgga
    11041 cagtctttct ttgacgaatc ttgcaaacta ttcccttttg cccaaattcg gaaatccttc
    11101 cagtatagaa ttggtagact ccgactttac cacgttaatc ctctactaca gtgaaagttt
    11161 cgatttatca ggctcttacc gtctcgtttt ttccacccaa ttgttcaatt gcataggcca
    11221 ggccataccg gaaaatatct attgggattt taatatccct tctccaagta agattttcga
    11281 tgtattgatt accgaagtta tggctgaccc cgatccaccc attcatctgc ccaatgcaga
    11341 atatgtcgaa atctacaatt gctgtgatta tacggtcgac ctttcgggtt ggagctttag
    11401 tatagagaat accgatcatg ttttcgatcg atattattta aaagccaacg aatatctcat
    11461 actcgcccac gaaaaatatg cttctgattt cgcaacttat ggaaatttct atgggtttag
    11521 tagcttttcg ctacctaaca ccgggcaatc gttggttttg aagaatggaa agggagaagc
    11581 cattcactgg gtcgactatg accagaatat gtatggtaaa tcacctaaaa aagatggagg
    11641 gtggtcgctc gaaatgatag ataccgaaaa cccctgtact attgagagca attggattcc
    11701 ttccattgac ccacatggag gaactcccgg acgaaaaaat tcggtttcgg cttcattgcc
    11761 cgatacgatt tctcctcgtt tttcacgaat ctatcctctc agtccgaaaa aaatatggct
    11821 ctatttttca gaaccggtaa aacaattcga agccactaat ccagagaatt atgtcttgag
    11881 tgatggactt actttcttga aaattcaagc catagatcac aggaataagg ttttcgaagc
    11941 cgaactttcg agcgagctta aacccgcaat tatttatacg gtttctgcta agaatggcat
    12001 taccgattgt gctggcaatt gcctttcaaa taataaggaa ataatatttg gtctacccct
    12061 gcgaccagcc agagggaagc ttgtcatcaa cgaaattttg acaaacccat tagagggtgg
    12121 aagcgattat ctcgaagtat ataattcctc cgatactttg agtgatcttg gagaactagc
    12181 cttaagttat atttcttcat caggaagctc aaccaccata tttcttccac cttttctttt
    12241 gttgccttcc gaatattact gtcttacacg caatcctcta tcagtaatca atcaatatcc
    12301 tctccatcaa aaagataact tcgtacaggt gaaaaatttt caagatctgt caagctcggg
    12361 tggtacgctg attttatatt taaaggaaga tagtacaaag gtgatcgatc gattttcgta
    12421 ttcatcgagc atgcatctcc caattcttaa aaacttcgat ggagtatcct tggaacgcat
    12481 caatccctta cggccgactg acgacccaac caactggact tcagcttcag aggcctcggg
    12541 atttggaaca cctgctcatg aaaactcgca atattcatct ttacctgttg ctaaaggcag
    12601 cttgaccata gaacccgaaa tattctcacc agacggcgat ggaattgacg atatagtgaa
    12661 tattaccatt caacctcctg ctgaaggtta tattacgaat gtgacacttt tcgattccag
    12721 aggcagatta gtaaaacatc tagtaaaaaa cctgattgta ggcaacaaat gtacaatttc
    12781 atggaacggc atgcgctacg ataattcgag agcacctgtg ggattttata tcatttattg
    12841 cgagttgttc gatactaatg gaagtaccga aaaacacaag gcaactgtgg tggtaggagc
    12901 taaactctga gaagctttat ccaaatctta cttcttacat aaaaatgaaa tttatacatt
    12961 cgaagaccaa ggttaatttt tatcagaaat tgtctgatat ttataataaa atgtcctgcc
    13021 atgataagaa tattttttga aaaacatact catgcttaaa ttcagaactc ttgatcccgt
    13081 aattgcctat cgatatttta caaaagtatt atcttagaaa cgctaacaaa aattatttca
    13141 aatcgttagt tcactttgcg cttatgcata caattctttt cccgctaaca ggctaagaac
    13201 cttttagatt ttaacgtcgg gaaaagaaat tcaaaaaaga attagcgctt gcagatgatg
    13261 ggagtcaacc tcaagaggat gaaaagaggg attatttaga gcaggtaatc ctttaacgcc
    13321 atcttcaaaa ccacgacaac ctgaataaaa ctgaatcagg atttttgaaa ctgacttggt
    13381 ggtttactgt ggtttgtcca acgaaccatt tggtgtcggg caagtcgaca tttaaaaaaa
    13441 tatatttaga tgcgcttgtt cgaagagagg cagagtaata gaaaaacata agaattatcg
    13501 aaaattccga cttcatcttc ttttcggtac agccaaaatc aaagcctggc gcagttcggg
    13561 ttcctttctc aaaacaataa tgaaaaacaa tacgaatagt atccctttta ttaccagatc
    13621 gagtccgagc caaggaagat gaatttttga acctatcaga aaaagggcaa tggaaagagt
    13681 tagatacagg aagaatttat cgagacgata cgggatgggg taatgttttt ggccataaat
    13741 aaacgacaaa agcatcatca aaagataaca gctgaagttt gcccaggcgc tgcccataaa
    13801 tccaattctg ggtataagaa aaatgttcag cccaatggtt actgcagttc caatcaggct
    13861 aaacaaagcc ccatacatcg ttttaccggt aattttatac caaactgaca gattatagta
    13921 aattcccaga aagacatgcc ccatgagtaa aaggggaact atgggcaggc ctgcgtaata
    13981 cataggcccg acgaaatact taatgacatc tataaacagc ataatgcctg cgaacattcc
    14041 aaaacagacg atgataaagt aattcatcac catagcgtaa gtctcgggag catccttttc
    14101 ctttgcctgc ttgaaaaaga aaggctcggc cgcataacga aaggcttgaa taaaaattac
    14161 aataaacaca ctgaatttga aacacatgcc ataaacacca agatcatgta tagcagtctc
    14221 gggaggaaga aaatgcttca atagtatccg gtcgatagtt tcgttgacaa ccccggccag
    14281 attatagaca aggagcggaa aagcatacag aaacatttcc ttccagagcc ctaagtcgat
    14341 agttaaactt gccttgcgga tagccggcca aagcagcagc aatgtggcaa agctcgccac
    14401 catgttcgaa ataaaaatcg cactcacctc gggtttccct cctgtaaata taccaagcca
    14461 gctccatcca tacttattgt ataaatatgg taacaacagg ataagaatga gatttagggc
    14521 aatattaatg aaaatattcg ccagttttat tccagcaaaa gtccatgcct tttgctgggc
    14581 tcgtagccaa gcaaacggaa tggcactcaa tgcatcgaat ccaagaataa gtaaaaaata
    14641 aatgatgtat tctgttttac cctcgtaacc aagccacgaa gcaatggaat cgcgaaaagt
    14701 aagggtaaac aacaggaaca gaatggttgt tatgacgatg gaattgaatg cagtactata
    14761 aaccatcttt ggttttgaat gggtttcgga atagcgaaag aaagccgttt ccatcccata
    14821 cgtaagaata acagccaaaa tggccatgta agcatatagt tcaacataaa cggccgattc
    14881 gtggggtaga aatactctgg tatataaggg aaccagaaga taattcaata tccttcctaa
    14941 tatgctacta agtccatata ctgcagtctg ccctgccaat tgctttactt ttcccattta
    15001 tcaggctgat atgaataatc cttcgatctc gaataatccc agaacgcaga tagaaaaatg
    15061 caaaaatggt attccatctg aaatatctat atttttctgt gatctggcca agtttctcat
    15121 caaactatat aggtaatata aatctttcat ttcgcgttat gtttttcatg ctatcacaag
    15181 gccgaaatgt aaaccgccaa ggtagtgccc ttatttttct ggctttcgac caaaatctca
    15241 acttgacgat aacccacgaa ttcttcagaa aaatcaaacc caaacccatt ggttcttcgc
    15301 catgagtgcc ggcggaagat cgagtgaaat caatccttga caaattttcc aagttttctt
    15361 cgtatatccc aatgccagaa tcggtaagtg acgaaaggat attttccttg tttttcccgt
    15421 cttttacgac gattcctcct cctgaatatg aaaaatccct agcactcgat aaaaaattat
    15481 tcatttcagt gggaatagaa ggttgattgt atcccacaaa agtaaggaca attaaacttt
    15541 attgcctggt tttttaaaag aagaaatgtt ttacaaactt acataaaaaa ataattttca
    15601 tacgtcgcac aacaaaaatc aaggccatga ttgttctata agtaatcaac gtactcaata
    15661 agtagtacaa ttgtttaatt taaaactatt tatatatgag aacagtcagt ggccttgacg
    15721 ggcacaaaga tagtgtattt gtatgctttt tggacgaaca agacaaaaaa attttagaaa
    15781 aacattacgg gacgctcact cccgatttaa cgaccttgcg tgatgcactg atagaacacg
    15841 attgtggaag cattgcaatg gagagtacca gcatttactg gatgcctgta tggcatgttc
    15901 ttgaatcaga cttttcgtta aaattggtca atccctattt catcaaacag ttacccggcc
    15961 gcaagagcga cgtaaaggat gctcagtgga tagctacagt tttacataaa gggcttttac
    16021 gcggcagtct gatcccctgc aaggagataa aaaagcttct aaaatataat cctgttacct
    16081 gggtcgcttt gtttcggagc ggaaacacaa tgcaccattt gctaaatggc ctgtaagttt
    16141 tattgcctgt tttgctgtaa gccctgcctt ctgtaataaa ccgacctata aacactgttt
    16201 tattaatgtt tcgtcctttg tttccttgtg gtggaactgg tcggagttat agccgatttc
    16261 gagtttttaa taatccagct tgcggctttc aatatactgt tttgctggcg ggctgttatg
    16321 cacggcgttt ttaaagtagg tgaacatctt ggttagtata tcagccttgc ttaccgcctg
    16381 ggctgtggct gccgggacgg catggccgtt caacattccg gctgctttta aaattgcatt
    16441 gtgtttatca tggttctccc gctacatgat aaaatcaata acatcaaggc ttttgccgtg
    16501 ggtcttgcaa ttggagataa aacagtatgc cgtgtgggtt ttagtagtaa acctacatgc
    16561 tcggggtctt atcttcgtgg aagaggcagc aga
//
LOCUS       DAOORU010000036        16528 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4272_length_16528_cov_5.216233, whole genome shotgun sequence.
ACCESSION   DAOORU010000036 DAOORU010000000
VERSION     DAOORU010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16528
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4272_length_16528_cov_5.216233"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            248..2635
                     /locus_tag="PLF20_03825"
     CDS             248..2635
                     /locus_tag="PLF20_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80172.1"
                     /translation="MKKKHCLFIIFIAVFFPQLVFSQQMTVKRAAYFRKSQPLSEMAA
                     VLPGARDRSWKDSLVRNLAFDEHHPIMHQLTPAQLDPVRQISAPSIPNAGPILNFAGI
                     GNVNSVYPPDTEGDVGRDYYFQMINLSFAIYDKQGNLLFGPVNNSTLWNGFIGPWTGT
                     NDGDPIVLYDELANRWIASQFAVNTSNGTYWQLIAISETSDPLGSYYQYAFEFPAFND
                     YPKFGIWPDAYYASFNLFGSYVRVAAAAFERDAMLVGDSNARMILFDLPPNAGAWSML
                     PSDFDGNPPPTGAPNYFAYFTDDGLGALQDAFKIWEFHVDWNNVNNSTFQPVTSLAVA
                     PFDAYFCDAPRGACIPQPNTNQGLETLSDRLMYRLQYRNFGTYQTMVACHSVDFDGQA
                     HAGIRWYEFRKTDSNPWEIYQQGTWAPDDLNRWMPSIAMNTIGDIALGYSVSSESYSP
                     SIRYTGRTANAPLGQMNLQEAEIITGSYAQSNFGRWGDYAMMAIDPSDDTSFWFTTEY
                     IKTGWKTRIAKIQFDTSFSVSAFAGPDTLICNIIPYNNLHSSAQNYRTVLWTTSGDGH
                     FNQPNEPHAIYFLGPQDKQSDSVKLTLTAFSYDLISVSSDSMTLFLHPSPYSYAGKDT
                     LICVGDPAYLHGTAYNTDSIVWSTYGDGYFSEPNLLNTIYFPGTNDINKGHVNLRLTA
                     MPTAPCFVPIESMMKVNIHVCTGTNEISKGNPVIEINPNPAHHIVKIHLKGFSHKTLS
                     LRVMDMNGNILFSRNFNSISSEIIQTIDLNLVPTGTYIIQVIQDQQVTQSKLIKN"
     gene            2753..2826
                     /locus_tag="PLF20_03830"
     tRNA            2753..2826
                     /locus_tag="PLF20_03830"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2787..2789,aa:Pro,seq:ggg)
     gene            complement(3102..3986)
                     /locus_tag="PLF20_03835"
     CDS             complement(3102..3986)
                     /locus_tag="PLF20_03835"
                     /inference="COORDINATES: protein motif:HMM:NF020476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HOX80173.1"
                     /translation="MTRRRAKADFPLVISASRMCDLPQWYPQYLIHEIKQRLETGRTL
                     HSLVIWTKHPLALLKSPLYDELSYLKSIGIQLYFQITITGMGNRVCGYDKFQRPWRPE
                     PIAPSKEESVKILPQLISLTEHPLRIRLRIDPILQVKDIKGEFYSNYEECKDTIEKCS
                     ALGIRKFSFSFVEPGIYRKVDRRFQQLGITLIKFDEILRNKIRYEFSDLEKKLGVDIS
                     GCCIEDWPVSSCINGRMLQQLHPQKLDVSLCQPHSRPLCGCTASIDIGGWPPKTCPTG
                     CDYCYARPFYRDLKEKEK"
     gene            complement(3994..6633)
                     /locus_tag="PLF20_03840"
     CDS             complement(3994..6633)
                     /locus_tag="PLF20_03840"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOX80174.1"
                     /translation="MKKTGKIILIVLIFLFILLLAIPILFKGQIKEAILKSASSMIDA
                     KLDFGKVNLSLIKNFPNLTVNVNKISLSGIKNFENDTLAFVGKASVTVDVFSAIHGKT
                     YEIKSVTIRHPRIYLKVLPDSSANWNIYHPSEDTTAPRNFTLKLKKIYIEDASLIYDD
                     RLLNTLVKAEGINSELSGDLTAESTSLQTFTLIKDIMVDYGGIRFLNKTECELKAEVD
                     ANLKDMIFTMKDNEVRINQLFLGFDGKVQMMEEGYGLDLSFSSRQTSFKNLLSMVPSI
                     YAKDFEKLTASGTMKFHGKIKGIYADTSFPSYDIVLEVNEGRFQYPSLPSAVENVAIR
                     ANVVNPDGQPNNLTLDLSRFHLEMAHNPIDLKVLLKNPISDPWIKAVILGRLDLAEIK
                     NVYPLGINLKGSIRADLSLEGNISTLEKGNFENFDAKGSLVIDDLFLPDTSTKKDLNI
                     STARLNFSPAYLELANCKINIGANDIAANGKVTNYLGYLLRNTDLKANLNVSSNRLNL
                     DEFVTSGSKSQNDKTQLGIIEVPKRIDFALNANLNQVVFGKLEMNDIRGEIQVKNQQA
                     FLDNLSLKTLGGLITLKGSYNTINPTHPCMELNLKIENIDIPLTASTFVTVQKITPIL
                     ENVKGLASASLSLKTDLDPNMIPDLNTLSSSGTIQTGNLKISNVAALQKLDDILKLNR
                     FKEFETNPLSLFYLIENGKLNIKPFDLKSGNLMGTLGGNVLIASQELDLDFIIQMPRS
                     EFGGAANSVLEGLVKQANQKGAKIKLGETVTIHAKITGQANNPKISTNLVESSKTLAS
                     DMKKQAEDEFNKKKIEIEQKAQAELEAQKQAAQARMKQEEERLRQETEQKKKLLEEKA
                     KRQADSLEKKGKDELKKRLKKVF"
     gene            complement(6685..7785)
                     /gene="aroC"
                     /locus_tag="PLF20_03845"
     CDS             complement(6685..7785)
                     /gene="aroC"
                     /locus_tag="PLF20_03845"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOX80175.1"
                     /translation="MTGNSFGRFFRLSTFGESHGEAIGGIVDGCPAGVEWNEKFIQKE
                     LDRRKPGTGFSSSPRQEPDHFEVLSGIYEGKTTGQPIAFLIRNIDQRPQDYEQAEQMI
                     RPGHADFAWKQRYGIFDPRGGGRASARETAARVAAGATAKLFLKKYGVHINAYVLAIG
                     PIQMTEKNWHPVEDIEADPLRCPHKETSSKMQDYLQQLNDEGDTAGGIIRCFVRNCPA
                     GIGNPVFDKLQAALAYAMMSIPAAKGFEYGEGFSAASMKGSQHNDTPEFKNGSIHFVT
                     HHAGGILGGISTGDDIVFNVAFKPVSSIDKPQLTVTYEGKPAIYQPGGRHDITVVPRA
                     VPVVEAMAALVIADQLIEWFALGNYLPENHSD"
     gene            8377..9651
                     /locus_tag="PLF20_03850"
     CDS             8377..9651
                     /locus_tag="PLF20_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008762539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HOX80176.1"
                     /translation="MVNSAPTTTNKSSYRLSISIIGALFFIFGFVTWLNGILIPYLKI
                     ACNLSDFQALFVTFSFYISYTIMALPSSWVLKKTGFRNGMMVGLWVMAIGTLIFIPAA
                     LTRTYGIFLTGLFIMGAGLALLQTASNPYITIIGPVESAASRISIMGIANKLAGAIAP
                     LILAYFILNDGDEFIRNLSTLDVQERIAALDGLARRVINPYIVMTVVLVLMGFMIRFS
                     PLPEIETEQEDEGTSILNSNKTNILQFPNLVLGVLTLFFYVGAEVIAGDTIIRYGLSL
                     GIKMEVAKAFTTYTMLSMILGYILGIILIPKYITQEKALQVSGLLGLAFSILILLTDG
                     YVSIIFVALLGLANALVWPAVWPLALHGLGRYIKTGSALLIMAIAGGAILPLAWGKLS
                     DMFDPNFAYIILIPCYLIIWLYALRLHKIKNW"
     gene            9698..10612
                     /locus_tag="PLF20_03855"
     CDS             9698..10612
                     /locus_tag="PLF20_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOX80177.1"
                     /translation="MKTKPTLLVLAAGIGSRYGGLKQLDPLGPHGETIIDFSVFDAMR
                     AGFGKVIFIIRKQIEKEFTNFFHDRYKGKMEVDYIYQELENLPPGYTVPVGRNKPWGT
                     GHAVLMAKDKINEPFAVINSDDFYGPGAYQAVIDFFSKPRSSSIPEYALVGYYLSRTL
                     SDYGGVSRGICQIDSNGYLKKIIETHQIHKTHGGYAYPNSGGDLVFLPGDTIVSMNTW
                     AFCPEIFQDFERLFLEFLKENHHSTSAEFYIPSVADHLISTGKARFKVLTVNEQWFGI
                     TYQEDRSKVVENLRKLVSEGYYPEPLWD"
     gene            10603..11679
                     /locus_tag="PLF20_03860"
     CDS             10603..11679
                     /locus_tag="PLF20_03860"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HOX80178.1"
                     /translation="MGLIKIPGEVFVAFGLDKSCSMVRPFGSGHIHQTFRVDTDNQCF
                     IFQCINHKVFPDVAALMDNIFKVTRYISKNSSPEDLTVRIIQTLEGKSFYHDDQGQYW
                     RVMERVPNSKTYDMALSPSMAFEAGKVLGDFHYLLKDFPVEQLHTILPNFHNLDSRWR
                     EFLIAKGKASTHRLSQANALIQIVDIACDDELIFSRKIYSPHTRKRIVHNDPKINNVL
                     FDNHLRGICMIDLDTMMPGSFLHDFGDALRTTANPFYEDEPDSTSVHCDLDLLKAYCQ
                     GYLPCVKKIMNTDEIMGLPHAPAHMTFIIALRFLTDYLNDDIYFKIDHPQHNLQRAKA
                     QFALYYDFKEREPEIEQIISQYVK"
     gene            complement(12021..13301)
                     /locus_tag="PLF20_03865"
     CDS             complement(12021..13301)
                     /locus_tag="PLF20_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HOX80179.1"
                     /translation="MIKRIPLYVKILAGLAIGIFVGIIAVITNSGGFIIDYVKPFGTI
                     FLNLLKLIAIPLIFTSLVSGIAGLSNIKKFSTLGVQTFVLYIVTTVIAVCIGLGLVNL
                     IRPGDYFSEGKRSEMMAKYGQSISIDEKNVQNLGHRSPLQFLVDMVPENIVDAASSNT
                     SMLQIIVFAVLFGLALISINEQFSEPVKKFIVGLNEVILKIVDFIMLIAPIGVLALMA
                     GLIVDVIGKDAAEGIQLFVSLGLYFLTVLVGIFIIIILIYPSILRIFVKVSFRQFYKA
                     IFPAQLVAFSTSSSAATLPVTMEQVEKELGIDREITSFVLPLRVTINMDGTSLYQAIA
                     SIFIAQVMGIDLSLSQMLTVLIMAVMASIGSAGVPGAGMVMLIVILNSIGIPAEGLAL
                     IIAIDRPLDMFRTVVNVTGDAMIATLLGKNRKIT"
     gene            complement(13625..14431)
                     /locus_tag="PLF20_03870"
     CDS             complement(13625..14431)
                     /locus_tag="PLF20_03870"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOX80180.1"
                     /translation="MNKKISSIVLNLACIAFLGSCSSTKKTNAPSDIFQTPSGVEIQI
                     IQHGKGKRAENGNIVRLHYTGKLTNGTIFDSSIQRDKPIQITLGKGQIIKGWEEALPY
                     LHEGDKAILSVPPELAYGNQDLGIIPPNSTLIFEIDLLEVVQSTPPEPFVTAGLDTLV
                     LDSGLKIIIVKKGNGPKASFGRQVVAHYTGYLPNGKVFDSSYLHGQPITFHPGSGQVI
                     KGWDEAFPLLPKGTKARLIIPPYLAYGDHDTGPIPAGSTLVFDVEVVDIK"
     gene            complement(14424..15728)
                     /locus_tag="PLF20_03875"
     CDS             complement(14424..15728)
                     /locus_tag="PLF20_03875"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOX80181.1"
                     /translation="MSIRHLRFILFIFVIGCIVYGCSRFSGYKKTSDGLYYKFYRQNE
                     GPHPDTSNIVQINLSYRYGDSVLFSSNILREPMNMMVNPPEFRGDLNQALMMMSPGDS
                     ASFLIRADSFFFCTLKYNRLPKISTPDSYVTLEVGMTKMWTKQEQRQKEQEWIIARKN
                     SEKEEIQRFIKEIRFEGDTTPEGLYFKITRKGEGPTPKPGDQVTLKFSVNLIDGTKLY
                     SSWDKGIPMTIEFGKKFDTEGIDIALSRMNKGSVARLIIPSKLAFGEKGRPPFIPPYS
                     PLLYDVEIVDIKSKIDKQKTNQEQVEKLRLSEQKRIEEYLVKNNLKVKPQANGLYFIE
                     LKKGQGVPPKTNQTVRVHYTGWLLDGTKFDSSYDKGKPFEFILGRGNVIEGWGKAVAQ
                     MQAGSKVKIILPSHLAYGERGAPPKIPPFAPLVFEIELLDYE"
     gene            complement(15750..>16528)
                     /locus_tag="PLF20_03880"
     CDS             complement(15750..>16528)
                     /locus_tag="PLF20_03880"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOX80182.1"
                     /translation="KTDSGLYYKFYVKSGDTAKPKVNDILKLYMSYGTKDSLLMDGKE
                     EFLLQLTNPQFKGDINEALAMMSKGDSASFIMPADSFFKKIVHVPLPDFIDSTEMLYF
                     NVKMIDFFSQEEFIKRMNEENALKEQKELSDLESYLSANNITVQPTESGLIYIEIVKG
                     TGPQATQGKKAKVHYTGTLLDGTKFDSSYDHPDKQPLEFIIGEQRIIPGFEEGVSLMK
                     KGGKAKLIMPSKLAYGSRPAGPSIPAFSTLVFEVELIDVQ"
BASE COUNT         4747 a         3437 c         3146 g         5198 t
ORIGIN      
        1 acatagcttc cgaaaaggcc tataatcatt actgcctgaa aaataagatt cgatagagca
       61 agcaaagatg aatcggaata tacattcgat gatatcaatt gaatctatcc atgcttagaa
      121 agaatctcct ttggaaaggt aaataataaa cttctccacc tttttgaaat caacctaact
      181 ttattatatc ttactgaaat gtttatgtaa atttgtgccg gataagaatt taccaatttt
      241 actactcatg aaaaagaaac attgcttatt catcattttc attgctgtct tttttcccca
      301 actggttttt tcacaacaaa tgacggtaaa gagagcagca tatttcagaa aatcgcagcc
      361 tttgtctgaa atggcagctg ttttgcccgg agcgagagat cgttcctgga aagattcact
      421 ggtgcgcaat cttgcttttg atgaacacca tcccatcatg catcagctca cacctgctca
      481 gctcgatccg gtaaggcaga tatctgcccc ttcgattccg aatgcgggtc ccatattgaa
      541 ttttgctgga ataggcaatg taaatagtgt ttatccacct gacaccgaag gagatgtagg
      601 ccgcgattat tatttccaga tgataaatct ttcatttgct atttacgata aacaaggtaa
      661 tttgcttttt ggtcctgtaa acaattcgac cctatggaat ggttttattg ggccttggac
      721 tggtaccaat gatggcgatc ctatagtcct atacgatgaa ttggccaata gatggatagc
      781 ctcacaattt gccgtgaaca catcgaatgg aacttattgg caacttattg ccatttcaga
      841 aacttccgat cctttaggtt catattatca atatgcattt gaattccctg ctttcaacga
      901 ttatccaaaa tttggcatct ggcctgatgc ttattatgct tctttcaatt tgtttggaag
      961 ttatgtaaga gttgcagctg ctgcttttga acgggatgct atgctggtgg gggattccaa
     1021 cgctcgaatg attctttttg atttgccacc taatgctggg gcatggagca tgcttccctc
     1081 cgatttcgat gggaatcctc ctccaacggg cgctcccaat tattttgctt atttcaccga
     1141 tgatggtttg ggagctcttc aagatgcctt caagatatgg gaatttcatg tcgattggaa
     1201 taatgtgaat aacagcacct tccaacctgt aacctctttg gctgtagcgc ctttcgatgc
     1261 ctatttttgt gatgcacccc gtggagcttg cattcctcaa cctaatacga atcaaggcct
     1321 cgaaacactt tccgaccgcc tgatgtatcg tctgcaatat cggaactttg ggacttatca
     1381 aactatggta gcttgtcatt cggttgattt cgatggacaa gcacatgctg gtataaggtg
     1441 gtacgaattc cgtaaaactg actccaatcc ttgggagata tatcaacaag ggacctgggc
     1501 tcctgatgat ctcaaccgat ggatgccaag tatagccatg aacacaatcg gagatattgc
     1561 cctcggatac tccgtcagca gtgagtcata ttccccttcc atacggtata ctggccgtac
     1621 ggctaatgct cctttaggac aaatgaacct ccaggaagca gaaatcatta ccggctccta
     1681 tgcacaaagc aactttggcc gatggggaga ttatgctatg atggccatcg acccctccga
     1741 tgatacttca ttctggttca ctaccgaata tataaaaacg ggctggaaaa ctcgcatcgc
     1801 caaaatccaa ttcgacacct ccttttcagt atcggctttc gccggacctg atacattaat
     1861 ttgcaacatt ataccttaca ataatctcca tagttctgca caaaattacc gtactgtact
     1921 atggacgact tctggcgatg gccattttaa tcagcccaat gaaccacatg ctatctattt
     1981 tttaggacct caagacaagc aatccgattc cgttaaacta accctcactg cctttagtta
     2041 tgacctcatt tcggtttctt ccgactcgat gacccttttc ctccacccta gcccttactc
     2101 ctacgctggc aaagacaccc tcatctgtgt gggagatcct gcctatttac atggaacagc
     2161 ttacaacacg gatagtattg tttggtcaac ctatggcgac ggctattttt ccgaacccaa
     2221 tctactcaac actatttatt tcccgggaac caatgatatc aataaaggac atgtaaatct
     2281 acgtttgact gccatgccaa ctgcaccctg tttcgtccca atcgaaagca tgatgaaggt
     2341 gaatattcac gtttgtacgg gtacaaatga aatctcgaaa ggcaatcctg tcatagaaat
     2401 caatccaaat ccagctcacc atattgttaa gattcatttg aaaggatttt ctcataagac
     2461 attatcattg agagtaatgg atatgaatgg caatatcctt ttttcaagaa atttcaattc
     2521 tatttctagt gaaatcatcc agacaataga tttgaactta gtgcccaccg ggacttatat
     2581 cattcaagtt attcaggatc agcaagtcac tcaatcaaaa cttataaaaa actaaataca
     2641 ccatcaattt tgtggtaaaa tttagagatt ttgtgaaggg gaaaagtttt cttttataaa
     2701 aatcatctaa atttgcaggc gcattcagga aggtcaatac tgaaatatag atcggggtgt
     2761 ggcgcagttg gctagcgtgc ttgcatgggg tgcaagaggt cgtgagttcg agtctcacca
     2821 ctccgacaaa ataagatcga taaaaaccgg ttcctgcttt aatgagaacg ggttttttta
     2881 tatttgatga ttacataata tacacggtaa caggtaattc aagattgaaa attggagatt
     2941 ggtgagttga aagttaacat actaaaaatg agcattttat atactttata gtgaagcctc
     3001 cttgcatctt gctctccttg tctcccagtc ccccttactc tcctcgtcct gctgtatcaa
     3061 tgtctgtata tttctatgca actcgcacca ggtatcgttt tctacttttc cttttccttc
     3121 aaatcccgat aaaatggtcg ggcataacaa taatcgcagc cagtaggaca ggttttagga
     3181 ggccagccgc ctatatcaat ggaagcagta catccgcaca acggacggga atgaggttga
     3241 cagagcgaca catcgagttt ctgaggatgt aattgctgaa gcatccttcc gttgatacac
     3301 gacgaaacag gccaatcttc tatgcaacat cctgaaatat ctactccaag ttttttttct
     3361 aaatcggaaa attcatatct gattttgttt cttaaaattt catcaaattt tataagtgtg
     3421 attccaagct gctgaaagcg ccggtctacc tttcggtaaa tacctggctc tacaaaactg
     3481 aacgaaaatt ttctaatacc cagggcggaa cacttttcaa tagtatcctt acattcctca
     3541 taattgctgt aaaattctcc tttaatatcc ttaacctgta gaataggatc gatgcgaagc
     3601 cttatgcgca agggatgttc ggtcaaggaa attagctgtg gtaaaatttt tacactttct
     3661 tcttttgatg gtgcaatagg ttcaggtctc catggtcgct gaaatttatc atatccacaa
     3721 acacgattac ccatcccggt gatagtaatc tgaaaataga gctgaatccc gatacttttc
     3781 aaataactca attcgtcgta tagaggggac ttcaagagcg ctaaaggatg ttttgtccag
     3841 ataacgaggc tatgcaaggt tcggccggtt tcgagtcgtt gttttatctc gtggattaag
     3901 tattgcgggt accattgtgg gagatcacac atccgtgaag cactgataac aagcgggaaa
     3961 tcagctttgg ctcttcttct ggtcataaat ttttcaaaag actttcttca atcgtttttt
     4021 tagctcgtct ttcccttttt tctcgagcga gtcggcctga cgtttggcct tttcttcgag
     4081 tagttttttc ttttgttcgg tttcttgtcg aagtctttct tcttcctgtt tcattctggc
     4141 ctgggcagct tgtttttggg cctccaattc tgcctgagct ttttgttcta tttcgatttt
     4201 ctttttattg aattcatctt cggcttgttt tttcatatcg ctggccagcg tcttcgaact
     4261 ttctaccaaa ttagtactta tttttggatt attggcttgt ccagttattt tggcatgtat
     4321 cgtgacggtt tctcccaatt tgattttagc tcccttttga ttggcttgtt ttaccaatcc
     4381 ttcgagaacg ctattggctg cacctccaaa ttcacttctt ggcatctgta taataaaatc
     4441 aagatcgagt tcttgagatg caataagaac atttccgccc agcgttccca tcagatttcc
     4501 cgattttaaa tcgaaaggtt ttatattgag ttttccattt tcgatgagat agaacaatga
     4561 taaggggttg gtttcgaatt ctttgaaacg gtttaatttg agtatgtcat ctaatttttg
     4621 caaagctgca acgttcgaaa tttttaggtt tcctgtttga atggttccac tcgaagaaag
     4681 tgtattcaaa tcaggaatca tattggggtc taaatcggtt ttcaaactga gtgaagcact
     4741 ggctaatccc ttcacatttt cgagtatagg ggtgattttc tgcacagtca caaaagtcga
     4801 agccgtgagt ggaatatcga tattttctat cttgaggttc aattccatac atggatgggt
     4861 aggatttatc gtattgtaag aacccttcaa tgtaatgagg cctcccaatg tttttaaaga
     4921 aagattatcc agaaaagcct gttgattttt aacctgaatc tcacctctta tatcattcat
     4981 ttcgagttta ccgaacacga cttgattcag attggcattc aatgcaaagt caatgcgttt
     5041 tggcacttct atgattccta attgggtttt atcattctga ctttttgagc ctgaagtcac
     5101 gaattcatca aggttaaggc gatttgaact gacgttcaga ttggctttca agtcggtatt
     5161 tcgaaggagg tatcccagat agttggttac ctttccattt gcggcaatgt cgttagctcc
     5221 aatatttatc ttacagttgg caagctccag ataggcgggt gaaaagttaa gccgtgccgt
     5281 tgagatattg agatcttttt tggtagacgt atcgggtagg aataaatcat ctattaccaa
     5341 actgccttta gcgtcgaaat tttcgaagtt tcctttttcg agagtagaaa tatttccttc
     5401 gagcgataga tcggctctaa tactaccttt aagattgatg ccaagtggat aaacattctt
     5461 tatttctgcc agatcgagtc ttcctaagat gacggctttt atccatggat cgctgatagg
     5521 gttttttagt agaactttca ggtcgatagg gttatgagcc atttcaaggt gaaaacggct
     5581 gagatcgaga gtcaggttgt tgggttgccc gtcgggattg accacattgg ctcgaatagc
     5641 tacgttttca acagcagaag gcaagctggg gtattgaaat ctgccttcat ttacctcgag
     5701 tacaatatca taagagggga atgatgtatc agcgtaaatt ccttttattt ttccatgaaa
     5761 tttcattgtg cccgaagcgg tcaatttttc aaaatccttg gcataaatag aaggcaccat
     5821 cgaaagcagg tttttgaaac tcgtttgccg gctcgaaaaa ctgaggtcga ggccataccc
     5881 ttcttccatc atctgaactt taccatcgaa tcctaaaaac agttgattta ttcgaacttc
     5941 attatctttc attgtaaaaa tcatgtcctt gagattggcg tcaacttctg ccttcaattc
     6001 gcattctgtt ttatttagga aacgtatacc gccataatct accataatgt ctttaatcag
     6061 ggtaaaagtt tgtaatgaag tgctttcggc agtcaaatca cccgaaagct cactatttat
     6121 tccttctgct ttgaccaaag tattcagcag gcggtcatca tagattaaac tggcatcttc
     6181 gatgtaaatc tttttcagtt tcagggtaaa atttcgggga gcagtcgtat cttcactcgg
     6241 gtgataaata ttccagttgg ccgaactatc gggtaaaacc ttcagataaa ttcgaggatg
     6301 acgaatcgta accgatttta tctcgtatgt tttcccatga atagccgaaa atacatccac
     6361 agtaactgaa gcctttccga caaaagccag agtatcattt tcaaaattct ttattcccga
     6421 aagacttatt ttattgacat ttacggttag attaggaaaa ttttttatta atgatagatt
     6481 taccttaccg aaatcgagtt ttgcatctat catcgaagaa gcactcttaa gaatagcctc
     6541 ttttatttga cccttgaaaa gaataggaat tgcaaggagc aaaataaata ggaaaattaa
     6601 aacaataaga attatttttc ctgtcttttt cattgtgaga agtgtttaat gttttcttaa
     6661 tctgattaat taatattgaa tttattaatc ggaatgattt tctggtaaat agttaccaag
     6721 tgcaaaccat tcgatgagtt gatcggcaat aaccagagca gccatagctt cgactacagg
     6781 tacggctctt ggcacgacag taatatcatg gcgtccgcca ggctgataga tagccggttt
     6841 accctcgtaa gttacggtca attgaggttt gtcaatgctc gaaacaggtt tgaaagcgac
     6901 attgaaaact atatcgtccc ccgtactgat tccgcccaaa attccgccag catgatgagt
     6961 aacgaaatga atacttccat tcttaaattc tggagtatca ttatgttgcg aacctttcat
     7021 gcttgcagcc gaaaatcctt cgccgtattc gaatcctttc gcagcaggaa tgctcatcat
     7081 ggcataagcc aaagcagcct gcagtttgtc gaataccgga ttgccaattc ctgcaggaca
     7141 attcctcaca aaacatctta taatacctcc tgcggtatct ccttcatcgt tcaattgttg
     7201 cagataatcc tgcattttcg aggaggtttc cttgtgtggg catcgcaagg gatcggcttc
     7261 gatatcttcg acaggatgcc agtttttctc tgtcatttga ataggtccaa tggctagtac
     7321 gtaagcattg atatgcacgc catatttttt cagaaataac ttggctgtgg cacccgcagc
     7381 tacacgggct gcagtttctc tggccgaagc tcttcctccg cctcgaggat cgaaaatgcc
     7441 ataacgctgt ttccatgcaa aatcggcatg ccccggacgt atcatttgct cggcctgttc
     7501 gtaatcttgt ggtctttgat cgatattacg gataagaaat gcaatgggct ggcccgtagt
     7561 tttcccctca taaattcccg ataatacctc aaaatggtca ggttcttgcc ttggagaaga
     7621 tgaaaaacca gtacctggct ttcttctatc gagttctttc tgaatgaatt tctcattcca
     7681 ttcgacaccc gccgggcatc catcaacaat acctccaatg gcttcaccat gcgattcgcc
     7741 aaaggtcgac aaacgaaaaa atcttccaaa cgaatttcct gtcatatttg caaagatata
     7801 tattgttaag ccctaatttt tattctatga ctcccctctg cctaactccc ccatttcatt
     7861 ctttcacttt caacgtcaat tgtttccaaa agattcgagt taaacctcat gatgagtact
     7921 gaaaaatttt cgacaaagca tattctcgtt tttaaaactt acataaattg caacgttttt
     7981 cccaataaag cgaaatatta ttctaataaa ttcattttca ataatcaaag caaaaatcat
     8041 atttattgat aaaaccgatt catttgaaaa ctactcatct ttatcgaaat acaaaaccac
     8101 ctcattatga atattcccaa ccgcaggttt cccgatttat tccaatgaaa ttcaatatga
     8161 tatacccggt tcatattctc attgccattc ttctattatt tccagcattt ccagattctt
     8221 ctttcaactt tatgagatta gttttggaca gtataattgt ttcgatttta tatttctctt
     8281 tgcactcgaa agttcatttt tataaatgaa ttgattctca tactattcag aatatcaata
     8341 aatcaatata aatcaacccc atcgatatca taaaatatgg tcaattctgc tcctacaaca
     8401 acaaacaaat cgagttatag attgagcatc agtattattg gtgcattgtt ttttatattt
     8461 ggtttcgtta cctggctcaa tgggatatta ataccctatt tgaaaattgc ttgtaatctc
     8521 agtgattttc aagccttatt cgtcactttc agtttttata tttcctacac aatcatggct
     8581 ttgccatcgt cgtgggtact caagaagact ggtttccgca acggaatgat ggtggggttg
     8641 tgggtaatgg cgattggaac tcttattttt attcctgcag ccttaactcg cacttatggt
     8701 atttttctga caggattgtt tattatggga gccggtttgg ccttgttgca aacggcttct
     8761 aatccttaca ttaccatcat tggacctgta gaaagtgctg ccagtcgcat aagtatcatg
     8821 ggaattgcca ataaacttgc cggagccatc gcaccactga ttcttgccta ttttattttg
     8881 aatgatgggg atgaattcat cagaaacctt tccacattgg acgttcagga acgtattgct
     8941 gcactcgatg gtttagctcg tcgggtgatc aatccttaca tcgtaatgac ggtagttttg
     9001 gtgttgatgg gctttatgat acgattttct ccactacccg agatagaaac cgaacaggag
     9061 gatgaaggaa cttccatact taattcaaat aaaaccaata tcttacaatt tcccaatctg
     9121 gtattagggg tactaacttt atttttctac gtaggagcag aagtgatagc cggtgatact
     9181 atcattcgat atggcctgtc gctcggcatt aaaatggaag tggcaaaagc ctttacaacc
     9241 tatactatgt tatcgatgat attggggtat atcttaggta ttatattgat tccaaaatat
     9301 ataacacaag aaaaggctct gcaggtatcg ggtcttttag gattagcctt cagtatcctg
     9361 attttactta ccgatggtta tgtttctatt attttcgtag ccttgctggg gctcgccaat
     9421 gcattggttt ggccggcagt ttggccactt gcgttgcatg gtcttggacg ttacattaaa
     9481 actggttcag ctttattgat aatggctata gccggcggag caattttgcc tttagcctgg
     9541 ggtaagttgt ccgatatgtt cgatcctaat tttgcttaca tcattctgat cccttgttac
     9601 ctcattatat ggttatatgc attgcgtttg cataaaatca aaaattggta ataagttttt
     9661 tattaatttc gaaccatcat tttcaatatc catagcgatg aaaacaaaac caacattatt
     9721 ggttcttgcg gcaggaatag gaagccgcta cggtggttta aaacaactcg atccgcttgg
     9781 gcctcatggt gaaactatta ttgacttcag cgttttcgat gccatgagag cgggttttgg
     9841 gaaagtgatc ttcataattc gtaaacaaat cgaaaaagag tttactaact ttttccacga
     9901 cagatataaa ggaaaaatgg aagtagatta catatatcaa gaactcgaaa atttacctcc
     9961 aggttatacg gttccagtag gaagaaataa accttgggga actggtcatg cggttttgat
    10021 ggctaaagac aaaataaacg agccttttgc cgtaatcaat tccgatgatt tctatgggcc
    10081 aggagcttat caggccgtaa tagacttttt tagcaaaccc cgaagttctt ctattcctga
    10141 atatgcactt gttggctact acctctcaag aacattatcg gattatggcg gtgtttccag
    10201 aggaatttgc cagatagatt ccaatggcta tctgaaaaaa attattgaaa ctcaccaaat
    10261 tcataaaaca catgggggtt atgcctaccc caattcggga ggtgatttag tgtttttacc
    10321 gggcgatacc attgtttcga tgaatacatg ggctttctgc ccggagatat ttcaagattt
    10381 cgaaaggctt ttcctcgaat tcttaaagga aaaccatcat tcaacaagtg cggaatttta
    10441 tattccctca gtagccgatc atcttatctc gaccgggaag gctcgattca aagtactcac
    10501 ggttaacgaa cagtggtttg gtatcactta tcaggaagat cgctcaaaag tcgtcgaaaa
    10561 tctccgaaaa ctggtaagtg aggggtatta tccagagcct ctatgggatt aataaagata
    10621 cccggcgagg tattcgttgc ttttggcctc gataaaagct gctcgatggt tcggcctttt
    10681 ggttcgggcc atatccacca gacttttcgt gtggatacgg ataatcaatg ctttatattt
    10741 caatgcatca accataaggt tttccctgat gtggctgccc ttatggataa tattttcaaa
    10801 gtaactcgat atatttcgaa aaattcctca cccgaagatt tgactgttcg gataattcaa
    10861 actttggaag gaaaatcctt ctatcacgat gatcaaggcc aatactggag ggtaatggaa
    10921 agagtgccca attccaagac ttacgatatg gcattatctc cctcaatggc ttttgaagcc
    10981 ggtaaagtac tgggagactt ccattatttg ctcaaggatt ttccggttga gcagcttcat
    11041 acgattttac cgaattttca taatctcgac agtcggtgga gggaattcct gattgcaaag
    11101 gggaaagcat ccactcatcg tttgagccag gccaacgctt tgattcaaat cgtcgacatt
    11161 gcctgcgatg atgaacttat attctcaaga aagatctaca gtccccatac tcgaaaaaga
    11221 attgtgcaca atgaccctaa aatcaataat gttctctttg ataatcattt aagaggaatc
    11281 tgcatgatcg atctagatac aatgatgcca ggtagttttt tacacgattt tggcgatgcc
    11341 ttgcgcacca cagccaatcc tttctacgaa gatgaacccg acagcacatc ggttcattgc
    11401 gatttggatt tattgaaggc ttactgtcaa ggttatttgc cctgtgttaa aaaaattatg
    11461 aataccgacg aaatcatggg tttgcctcat gccccggctc acatgacatt tattattgca
    11521 ttgcgttttc ttaccgatta tctgaatgac gatatttatt ttaagataga tcatcctcaa
    11581 cataaccttc aaagagcaaa ggcacagttt gcgctgtatt atgattttaa agaaagagaa
    11641 cctgaaatag agcaaataat atctcagtat gtaaaatgat tttactgaat taggggtatt
    11701 aaactgttaa tcaattgctt cattcaaatc tttaggaaaa ctattatagg gagcgacctt
    11761 caaaacccat caccattttt gtcttcataa ttaaaattca gaactacaaa ttattttatt
    11821 ctttctttgc tttggctttt gaggtaaact cacatttgtc aaacaaccgg tgttttttca
    11881 taagaggaac accagagaag gtataaagaa ttacagagaa taaataccta aagcaaaaag
    11941 atcatcaaag aatcttgccg tgagctccat aatcctcatt ccgcaatggg taccgctcaa
    12001 cttgcatttc gattacatat ttaggtaatt tttctgttct ttcccagaag agtagctatc
    12061 atggcatcac cggtaacgtt cacaacggtt ctgaacatat cgagaggacg gtctatcgcg
    12121 ataattagtg ctaaaccttc tgccgggatt cctatactgt tcaatatcac aatcaacatc
    12181 accattcctg ctcctggaac tcctgcagag cctattgaag ccattacagc catgatgaga
    12241 acggtcagca tttgacttag agagagatct attcccatga cttgtgcaat aaatatggaa
    12301 gcgattgctt gatacaggct tgtaccatcc atgttgatag ttacccgaag gggaagcaca
    12361 aaactcgtta tctctctatc tatgcccaat tctttttcga cttgttccat agttacaggc
    12421 agagtagcag cactcgaact cgtggaaaat gcaaccagtt gagccggaaa aattgctttg
    12481 taaaactgcc tgaatgacac cttgacaaag atccttaaaa tggaagggta tatcaagatg
    12541 ataataatga aaatgcctac cagcaccgtc aaaaaatata ggccaagtga aacaaaaagc
    12601 tgaattcctt cggctgcatc ttttcctata acgtctacta tcaagccggc cattaaggct
    12661 aagaccccta tcggggctat cagcatgata aaatcgacaa ttttaagtat gacttcattg
    12721 agcccaacaa taaacttctt tacaggttcg gaaaattgct cattaatgct gatcaaggcc
    12781 aatccaaaaa gcacggcaaa tacgatgatt tgcaacatgg aagtattgct tgaagctgca
    12841 tccacgatgt tttcaggtac catatccact aaaaattgta aaggggatct gtgtccgaga
    12901 ttttgaacat tcttctcatc gatggaaata gactggccat attttgccat catttcagaa
    12961 cgtttgcctt ccgaaaaata atcaccagga cggatcaaat tcaccaatcc caacccgata
    13021 catactgcaa taacagtagt tacaatatat agaacgaagg tttgtactcc taatgtagaa
    13081 aacttcttaa tattggataa tccggctata cctgaaacca atgaagtaaa aatcaatgga
    13141 atggcaatca atttcaaaag atttaaaaag atcgttccaa atggttttac ataatcgata
    13201 ataaacccac cgctattggt aattacagca ataataccga caaaaattcc aatggcgaga
    13261 cctgctaata ttttgacata tagaggaatt cgttttatca taactttttc ctgatcaata
    13321 caatccgata atacttatta tagaattttc aatgcttcaa cccaaaaaat taaggttcga
    13381 atcagtttct gtcctattca tccgcaaaag taggaacttg gccaaactat aacaaaagtg
    13441 gatttgtttt acatagaaac gagaggatga agatcggtga aagataaata tgaaattgaa
    13501 agttgtacaa atattgagca cggttaactt ctttcgagca ccgaaattat tattgaatat
    13561 tttacaagaa aacatcgaat cgaatgacaa gctgcaaagc gattcaatat gaacagggtt
    13621 atccctattt tatatccact acttctacat cgaagaccag agtcgaaccg gctggaatag
    13681 gtccagtgtc atgatcacca taggccagat aaggagggat gattaatcgt gcttttgttc
    13741 ccttgggcag taaaggaaaa gcttcatccc aacctttgat aacttgaccg cttcctggat
    13801 gaaaagtaat gggttgacca tgaagataac tgctatcgaa tacctttcca ttgggtaaat
    13861 aaccagtata atgtgcaacg acctgacgac cgaaactcgc cttcggccca ttgccttttt
    13921 ttacgatgat gatcttcaat cctgaatcca gcaccaaagt atccaatccc gcagtaacaa
    13981 aaggctcagg aggcgtagac tggaccactt cgagcaagtc gatttcaaaa attaaagtag
    14041 aatttggagg gattatccct aaatcctgat tcccataagc cagttcggga ggaacagaaa
    14101 ggatcgcctt atctccttcg tgcaaatatg gcaaggcttc ctcccatcct ttgataattt
    14161 gacccttgcc caaggtaatt tgaatcggtt tatcgcgttg aatgctgctg tcgaaaatag
    14221 ttccattggt aagtttacca gtgtaatgca atcggacgat atttccattt tctgctctct
    14281 ttcccttgcc atgctgtata atttgaatct ccactcctga aggggtttga aaaatatcgg
    14341 agggagcatt tgtttttttt gtgctgctgc atgagcccaa aaaagcaatg caagccaggt
    14401 ttaatacaat ggaggatatt tttttattca taatcgagca attcaatttc gaagactaaa
    14461 ggcgcaaaag gaggaatttt aggaggtgct ccacgttcac cataagcaag atggctcggc
    14521 aaaataattt ttacctttga gccggcttgc atttgagcaa cggccttccc ccagccctcg
    14581 ataacatttc cacgccctaa aataaattcg aaaggttttc ctttatcata tgaagaatcg
    14641 aacttcgttc catccagtaa ccagcctgta taatgcaccc gaaccgtttg attggttttg
    14701 ggaggaaccc cctgcccttt tttcaactct ataaaataaa gtccattggc ctgtggcttc
    14761 actttcaaat tgttcttaac caaatattct tctattcgtt tctgctcact cagacgaagt
    14821 ttttccactt gctcctgatt ggttttttgt ttatcgattt ttgattttat gtcaactatt
    14881 tcgacatcat aaagcaaagg agaataaggg ggtatgaaag gagggcgacc tttttctcca
    14941 aatgctaatt tgctcggaat tatcaatcga gcgacagaac ctttattcat tctcgataag
    15001 gcaatgtcga ttccctcagt atcgaatttc ttcccaaatt caatggtcat tggtattcct
    15061 ttatcccacg acgagtacaa tttggttcca tcgatcaaat ttacactgaa tttcaatgta
    15121 acctgatcac cgggtttagg agttggccct tcaccctttc gagtaatttt aaaatacaaa
    15181 ccctcgggag tagtatctcc ttcgaatcga atttccttaa taaatctctg aatttcctcc
    15241 ttttcggaat tttttcgagc tatgatccat tcttgttcct tttggcgttg ctcttgtttt
    15301 gtccacatct ttgtcatgcc tacttcgagt gtaacatacg agtcgggggt gcttatcttt
    15361 ggcagacgat tgtatttcag agtgcaaaag aagaacgaat cggctcgtat aaggaatgaa
    15421 gcactatcac ctgggctcat catcattaaa gcctgattga ggtcgccccg aaattcagga
    15481 ggatttacca tcatattcat gggttctctt aaaatgttgc tgctaaataa tacggaatcc
    15541 ccgtagcgat aacttagatt tatctgtacg atattactcg tatctggatg cgggccttca
    15601 ttttgtcggt aaaacttgta atatagaccg tcgctggttt tcttataacc actaaatcgg
    15661 ctgcacccat aaacgatgca gccgatgaca aaaataaata aaatgaaacg taaatggcga
    15721 atgctcatat catgcaaaaa cgttcaaaat tattgaacat caatgagttc aacctcaaat
    15781 accagagtag agaatgcagg aatactaggc ccagccggac ggctgccata agcaagcttg
    15841 gaaggcataa tcaatttggc tttgcctcct tttttcatca aacttacacc ttcttcgaaa
    15901 ccaggaataa tacgctgttc acctattata aattctaaag gttgtttatc aggatgatcg
    15961 tatgatgaat cgaacttggt tccatcgagc aaggttcctg tataatgaac cttggccttc
    16021 ttcccttggg ttgcttgtgg accagttccc ttaacgatct cgatgtagat aagcccgctc
    16081 tcggtaggtt gcaccgtaat attattagcg ctaagataac tttccagatc gcttagctcc
    16141 ttctgttctt tgagcgcatt ttcctcattc atacgcttga tgaattcttc ctgactgaaa
    16201 aaatcgatca tttttacatt aaagtaaagc atttctgtgc tgtcgataaa gtcaggcaac
    16261 ggaacatgta ctatcttctt gaaaaatgaa tcggcaggca tgatgaaact tgcactatcg
    16321 cctttcgaca tcatggcaag ggcttcattg atatcaccct tgaattgtgg gttggttaac
    16381 tgcagaagaa attcctcttt gccatccatc aaaagactgt ctttagtacc atacgacata
    16441 taaagtttca agatgtcgtt aaccttaggt ttggcagtat cgcccgactt gacgtaaaac
    16501 ttgtaataca aacctgaatc ggttttct
//
LOCUS       DAOORU010000037        15965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4453_length_15965_cov_5.470459, whole genome shotgun sequence.
ACCESSION   DAOORU010000037 DAOORU010000000
VERSION     DAOORU010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15965
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4453_length_15965_cov_5.470459"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="PLF20_03885"
     CDS             complement(<1..255)
                     /locus_tag="PLF20_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80183.1"
                     /translation="MNLIEIVSAAIPLVIAGGLIGIIKYKFPKGNISSLKYAFIWGII
                     AGLASVFIQYLAHWMNLDSLRSLKRMTFFAFIVIGGGQEFF"
     gene            617..1573
                     /gene="rsgA"
                     /locus_tag="PLF20_03890"
     CDS             617..1573
                     /gene="rsgA"
                     /locus_tag="PLF20_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOX80184.1"
                     /translation="MNHICIMEQDGSVIRSTGSHYTVMFADGRTMECRLKGKFRTKGL
                     KNTNPVVVGDKVRVVTDEKHQIGIITEIYPRTNYIIRKAPRSSSPISILAANIDQALL
                     VVTLHRPPTSTGFIDRFLLTAEAYEIPVYLVFNKMDLYNDDDLELLDTFAKYYASCGY
                     GIIFTSVVMHQGLDELKEVLHNRTTLLAGHSGVGKSALIHAIEPSLNIKIGEISAYHK
                     KGKHTTTFAQMHPLSHGGFIVDTPGIKEFGIIDFKKEEVALYFPEMRKLLPQCQFYNC
                     THDHEPGCAVKQAVKDGEIASWRYHNYLSVIHGEEIENAFSS"
     gene            1585..2247
                     /locus_tag="PLF20_03895"
     CDS             1585..2247
                     /locus_tag="PLF20_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOX80185.1"
                     /translation="MLAKNILNSLILPLEIHQTGYDALNRMDDYHIGQLPVISNGEYF
                     NLIREDEIFEYNLPDTPLKEFTFSSRIPPALSNQHLLEVLEIMAEYNLEIIAVVNEKN
                     MYQGCISLYDILKALYHLDMIQNPGGILVLELNLHDYSLTQIAQILEANDAKVLGLYT
                     ISPPDSTKVEVTLKINLLDLSPIIATFNRYDYIIKASFGQSDWDDLLRDRYDSLMNFL
                     NI"
     gene            2255..3646
                     /locus_tag="PLF20_03900"
     CDS             2255..3646
                     /locus_tag="PLF20_03900"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOX80186.1"
                     /translation="MKRIVLFIACLSLSGYCIHAQSVTHKWIVDTILIEGNHITKASV
                     IKREIVVKPGDSVAETHIPLILERSRDNLRNISLFNFVDIDTMNLGINHFALKVSVVE
                     RWYVWPYPIFEIADRNFNTWWRARNFDRLNYGIKILWDNFRGSNQTLSICTRLGYDHK
                     ITFEYIVPFIDKKRRLGLSFGFSTGGIHEAAALTNLNNEVDFIKTDERYIKTDHQAFV
                     AFTYRRNFNISHRLEASYNHYHAEDTLVKFNPMYGPCNVSYLSVSYLFKADYRDYKHY
                     PLKGWYMDLEVDKYGLGILDDGNIDLWKFSANYRIFSPISNKLYLAFGLYSSFQPDIL
                     PPYPLNAGLGYGRYFVRGYEYYVIEGYWQSLAKTTLKYTLIPQKITRLHGIKSEKFSK
                     LHYAFYLNLFSDLGYSKPWSELTPTRLNNQLLYSIGIGLDFVTYYDKVLRLECTLNHK
                     GETGIYLHFMAAI"
     gene            3897..4448
                     /locus_tag="PLF20_03905"
     CDS             3897..4448
                     /locus_tag="PLF20_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOX80187.1"
                     /translation="MAFILSVNGISPVIGKNVFLAPNATIAGDVVIGDCCTVWFNAVV
                     RGDVHSIRIGNNVNIQDGAIIHCTYKKSPVNIGNNVSIAHGAIVHGCTIHDNVLIGMG
                     AIVMDDAIIESNSIVAAGAVISKNAIVKSGTVWGGVPARQIKEIDTELLEGDVLRIAN
                     AYNMYASWYAENWETDGKPRNRI"
     gene            4737..5606
                     /locus_tag="PLF20_03910"
     CDS             4737..5606
                     /locus_tag="PLF20_03910"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HOX80188.1"
                     /translation="MKIALFGKNIIENHHSFLEKLLQRLHDLDCHILIYEQWKSPIET
                     IRKSGIPYDTFATYHDLRSADYLLSIGGDGTLLDTLPLVHNTGIPVLGINIGRLGFLS
                     AVSRDEIETVLDCIINKKIMLEPRTLLEIKSDDYDGYKFALNEISILKQLPASMLSIK
                     TFIDGEFLNTYWADGLIVATPTGSTAYSLSCGGPILMPSSENFVITPVANHNLTVRPV
                     VVPDSSKIDIEVDKKAGKFLLGLDSRITSFSAGGKIILKCADFKLNLGRLPDRNFCTT
                     LRAKLTWGHDIRN"
     gene            5650..6525
                     /locus_tag="PLF20_03915"
     CDS             5650..6525
                     /locus_tag="PLF20_03915"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HOX80189.1"
                     /translation="MSNEEYRFWKKSIDAMYLVKRNFITLLLLWMVVGTNAQYLESGI
                     TAGGSLYTGDLNPSPALSLTQPAAGILLRYNFTTRWVWKNSLLFGKLKGDDTKGENLW
                     GRNLQFESNLTELSSQIEFNFLNYFTGSHRENFSPYLFAGLSLFMFNPKAPFEGNWTE
                     LQPLNTEGQDTKAFPDRKPYSKISMAMPFGLGIKYGIGKRLCIGLETGYRKTLTDYID
                     DVSTTYYLEGDKIDATIPEEILSDPLRNHSPGMQRGNSRNKDWYAYGGIFITYKFNLY
                     KAPPCNDFKNIQRFE"
     gene            6658..7422
                     /locus_tag="PLF20_03920"
     CDS             6658..7422
                     /locus_tag="PLF20_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HOX80190.1"
                     /translation="MSETSRLQEIKASLDMGKLPRHIAIIMDGNGRWARQRGMDRVMG
                     HQHAITAVRETAEAAAELGIRYLTLYTFSTENWKRPQCEIDAIMSLLVETIHNETDTL
                     TKNNIRLRAIGDLDQLEKSTRQALENAIEITSSFTRMDLVLAISYSARWEITEMVKKI
                     AREVENGTLLPSQITQEIISAYLNAPFLPDPELLIRTSGEYRISNFLLWQIAYTELYF
                     TPVLWPDFRKEDLFLAIADYQRRERRFGKISEQLSS"
     gene            7466..10105
                     /locus_tag="PLF20_03925"
     CDS             7466..10105
                     /locus_tag="PLF20_03925"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOX80191.1"
                     /translation="MKSNRFILPIAFTLFIYFVPFYVQAQITIGSPQVSIDYDNPKEY
                     EIGGITITGVQYLDASVLTMLTGLKIGDRIKVPGEEITLAIQKLWRQGLFDNVRIIAQ
                     RIEDNLIFLEIQLVERPRLSKFTFIGIKKGEADDLRDKLKLASGEVVTRSSLVRAEET
                     IKKYFTDKGFLNVQVDIQEIRDTTKLNNVILKFTIDKKQKVKINEIIIHGNQQVKTST
                     IKNALKETKEKSAFRPLGGLDQLVWNTVKNAAKLDMAGLTDNFIDYYYTNLKLRIFKS
                     SKFIREDFETDKENIIKKYNELGYRDAEIVKDSIYFVDNNNLNIELWINEGHKYYFRN
                     ITWVGNTKYSSDVLNAYLKIRKGDVYNQQMLQTNLSYNPNGFDVSSLYLDDGYLFFNI
                     QPVEVNVENDSIDLEIRIYEGKQATLNRVTVKGNDRTSDHVIIRELYTKPGQLFSRND
                     IIRTQRELAQLRYFNPEKLGVDYTPNPEDGTVDLEYTVEETSSDQIELSAGWGYGRLV
                     GTLGLSFNNFSMRRIFDKNAWKPIPSGDGQKLSLRFQTYGVGYTSYSASFTEPWLGGK
                     KPNAFTVSYYHSKYTNGLKSSDTLYASFIIDGLGFSLGKRLEWPDDYFQIYHSINLQR
                     YRLINYPNIFTFGTGNGEYNNFSYTISFGRSSDDSPIFPRRGSTVNLSLELTPPYSLF
                     SNKDYSSMSAEEKYKWIEYHKWKFYGNWYSQLVGNLVLATKYRFGFLGYFNKEIGVTP
                     FERFYVGGDGLSGYGSLDGREIIALRGYSNESITPEYFENNGVGGTIFSRYVFELRFP
                     LSLNPNSTIYALAFMEGGNTWNKFTSFNPFKVYRSAGLGLRIFLPMFGVLGLDYGYGF
                     DEVPGIPSANGGQFHFSINQSID"
     gene            10164..10700
                     /locus_tag="PLF20_03930"
     CDS             10164..10700
                     /locus_tag="PLF20_03930"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOX80192.1"
                     /translation="MIRKRLALLFTAIMLAIGVSAQKFAYVDSEYILQNIPEYNDALA
                     QIDEFAAQWQQELEAKFAAIDKMYKDYQAEAVLLPEEMKRKREDEIMKAEKAAKDLQK
                     QRFGKDGDLFKKRQELVKPIQDKIYSAIERIAQAKNYAMIFDKAGNVTVMYADAKYDI
                     SDEVLEEMGYGFNTRKNK"
     gene            10852..11364
                     /locus_tag="PLF20_03935"
     CDS             10852..11364
                     /locus_tag="PLF20_03935"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOX80193.1"
                     /translation="MKKTFVKFFVLATCVFAFGGVSAQKFGHIDFAKLFVVMPGQDTI
                     QEKYRQYADGLRVQMEAMQTEWETKITDYQAKSSSMTDLIRRTKEKELQDLQSRIEAF
                     QQQASIDLQNKETELTSPLIERAKKAIQDVAKENGYTYVFNSAEGLILVAPPSDDILP
                     LVKKKLGIKE"
     gene            complement(11430..12524)
                     /gene="dprA"
                     /locus_tag="PLF20_03940"
     CDS             complement(11430..12524)
                     /gene="dprA"
                     /locus_tag="PLF20_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOX80194.1"
                     /translation="MSLLYEIALTLIPGVGDINGKKLVNYCGSAEAVFKEKKSALMRI
                     PGIGEYTANAILNTKPFDRAQQEIEFIKKYKVNVFYYKDKNYPLRLRNCEDAPILIYY
                     KGTESLNPPLSLSIVGTRRATDYGKWYIDKLMEALSSYSVVIISGLAFGIDTAAHRSA
                     LEHGLPTIAILGHGLDRIYPSQNRQLAKKILDHGGLLTDFPSQTKPDRENFPKRNRII
                     AGLCDALLVIETGIKGGSLITADIANSYNRDVFALPGRFGDQMSEGCNWLIRTNRASL
                     LDSPQVLLDDLGWAQVEGGTRTAQSKLFINYTEDEKKIVEILNRDSTVSIDKICIESQ
                     LPSTKVVAALLNLEFQGIVKCLPGKVYQLI"
     gene            12856..13926
                     /locus_tag="PLF20_03945"
     CDS             12856..13926
                     /locus_tag="PLF20_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80195.1"
                     /translation="MKRVLIAPLNWGLGHATRCVPLIEAFLKRNVEIILAGEGLVFDF
                     LRHRYPFLPIINLPGQRITYQIRGSLILALAKSFPGFLRNIELEHHIIKDLIEKYRLG
                     AIISDNRYGLWDENIPSFMITHQINVRGKGLWRIGEPFAHAGIRRFLNHFHEIWIPDF
                     PQFPGLAGDLSHDKSPRLKYRYLGPLSRFEHAAPIYEHPQNDIMAIISGPEPQRTIFE
                     NILTRTFEGTNLKTIILRGLPSHQESIVKINRITLYSHLQDDDFIEVLKNSRHLVCRS
                     GYSSLMDLTQLERTALLVPTPGQPEQEYLARLSSHSGYFTTIPQNQFHLDNFPDMPSL
                     PCFHTINREYLDKACEMVIGLL"
     gene            complement(14074..15843)
                     /locus_tag="PLF20_03950"
     CDS             complement(14074..15843)
                     /locus_tag="PLF20_03950"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HOX80196.1"
                     /translation="MEIMDTGSKKIRMIFYRFTMLMISIIVMVHSLWSQDTLLTYCNP
                     LNLDYVYCPIPDFTQQGRHRTTADPLIVPFQGKYFLFSTNQHGYWWSDDLARWNFIPR
                     KFLLPRHRVYDELCAPAAFVLGDSLLLIGSTHNPDFPIWMSHHPTGDEWKIAIDSFPV
                     GAWDPAFFADDDGKLYLYFGSSNFYPIYGCEIDRKTLKPRAEPEPLIRLNDSLHGWER
                     FGEHNDNTFLRPFIEGSWMNKHLGYYYLQYAAPGTEFSGYADGVYVGLHPLGPFQYQD
                     LNPFSYKPGGFARGAGHGATFCDYNQQWWHISTITIGVKNNFERRIGLWPAYFDPDGI
                     LYAETAFGDFPHYYNGKFTGWMLLNYQKPVTVSSTLGGYYPNFAVDEDIRTYWSAKSG
                     KAGEWICTDLSDTCVVYAFQINYADQDVPFLGKTSGLYYQYRIWGSLNGKDWTMICDK
                     SDNRTDVPHDYVALESPVELRYLKLENLHIPGGKFALSGLRIFGKANILPPTQVEYFT
                     ILRGHSEPRNAWLKWNRKDDATAYLIRFGLEPDKLYGSIMVFGENEYYFTGMDRNKTY
                     YFTIQAFNEGGCGPQSQTVESSP"
BASE COUNT         4961 a         3327 c         3109 g         4568 t
ORIGIN      
        1 gaaaaattcc tgtcccccac ctattacaat gaaggcaaaa aaagtcattc ttttgaggct
       61 tcgcagcgaa tcgagattca tccaatgagc cagatattga atgaacaccg aggcaaggcc
      121 ggctattatt ccccagataa atgcatactt caatgaagaa atattgcctt tgggaaattt
      181 atatttaatg ataccaatca gtccccccgc aatgacgagt ggtatagctg cagaaacgat
      241 ttcaattaaa ttcatgagtg ctcctcctgt ttgttgttca atttatttta tcactcaaag
      301 ttatgaaata ttttttccat gtaacatttc tcgaaaaatt tttaaaaata attttttaac
      361 ctcaaaactt tgacgatttg tgcaaatttt gtaacttcag tgttgtgttt caaagaattc
      421 atcaaaggtt gctatttgcc ctgatgatgg ataaaagcca aaacccgaat attatttcca
      481 tgcatgagat gggaaaattt agcgagaagc aataccgcag aacagcgtca tttaacaaat
      541 tggacttcta aatttatatt gataatcaac aagatatatg attattgaca atttatatgg
      601 caaaagttta gaataaatga accatatttg catcatggaa caagatggca gcgtcatacg
      661 gtcaacagga agtcattaca cggtaatgtt tgcagatggt cgaacaatgg aatgcaggct
      721 gaaaggaaaa ttcagaacaa agggattgaa aaacaccaat ccagttgttg taggtgacaa
      781 agtacgcgtt gtaacagacg agaagcatca aattggcata attaccgaaa tctatccacg
      841 tacaaattat atcatacgca aggccccaag gtcgtcatca cccattagca ttcttgctgc
      901 caacatcgat caggctcttc tggtagtaac gcttcatcga ccccctactt ccactggatt
      961 tatcgaccgt ttccttttga cggcagaagc ctatgagatt ccagtttatc tggtttttaa
     1021 caaaatggac ctatacaacg atgatgatct cgagctattg gatacttttg caaaatatta
     1081 tgccagttgc ggttatggga tcatttttac ctctgtcgtt atgcatcaag gattggacga
     1141 actcaaggaa gtgcttcaca atagaaccac tttactggca gggcattctg gtgtagggaa
     1201 atcggcactt attcacgcca tcgaaccctc gttgaacata aagattggcg aaatatcggc
     1261 ctatcataaa aaagggaaac acactacaac ctttgcccag atgcatcctc tttctcatgg
     1321 tggctttatt gtcgacaccc caggcattaa agaattcggc atcatcgatt tcaagaaaga
     1381 agaagtggct ttgtattttc ctgagatgcg taaactattg ccgcaatgcc aattttacaa
     1441 ctgtactcac gatcatgaac ccggctgtgc cgtaaaacaa gccgtcaaag atggtgagat
     1501 tgcctcatgg cgctaccata attatctcag cgtgattcat ggcgaagaaa tcgaaaacgc
     1561 cttttcatct taaaaccctt gtttatgctt gccaagaata tactcaattc actcattctc
     1621 cctctcgaaa tccatcaaac cggatacgat gctctcaatc gaatggatga ttatcacata
     1681 gggcagcttc ccgtaatttc taacggagaa tatttcaatc ttattcgtga agatgaaatt
     1741 tttgaataca acctgcccga tacaccactc aaagaattta ccttttcttc gcgtatccct
     1801 cctgcattat ccaatcagca tctcctcgaa gtcttggaga tcatggccga atataatctc
     1861 gaaattattg ccgtggtcaa cgaaaaaaat atgtatcaag gctgcatcag tctgtacgac
     1921 atcctaaaag cactctatca tctcgacatg attcaaaacc caggaggaat tcttgtgctc
     1981 gaacttaatc tgcacgatta tagcctgaca cagatagctc aaatcttgga agccaacgat
     2041 gcaaaagtcc tcggacttta taccatttca cctcccgata gcaccaaagt ggaagttacc
     2101 ctcaaaatca atctgcttga cctcagccct ataatcgcta cttttaatcg ttacgactac
     2161 atcatcaaag catcgttcgg acaaagcgat tgggatgatt tactgcgcga tcgatacgac
     2221 tcgctcatga attttctgaa catctaacag cggcatgaag cggatcgtgc ttttcatagc
     2281 ttgtctgtca ctttcaggat attgcattca tgcacaatcg gttactcata aatggatagt
     2341 tgataccatc cttatcgaag gaaatcatat caccaaagcg tcggtaataa aacgtgaaat
     2401 cgtagttaaa ccaggcgata gtgtggctga aactcatatt cctcttattc tggaaagaag
     2461 cagagataat ttacgcaaca tttctctttt taattttgtc gatattgaca ctatgaattt
     2521 gggcattaat cacttcgccc tgaaagtatc ggttgtcgaa agatggtatg tatggcctta
     2581 tcctatcttc gaaatcgccg accgtaactt caacacatgg tggagagcca gaaatttcga
     2641 caggttgaat tatggtatca aaatcttatg ggataatttt agaggaagca atcagactct
     2701 ttctatctgt acccgattgg gatacgacca taaaattaca ttcgaatata tagttccatt
     2761 tattgataaa aagcgaagac ttgggttatc gtttggattc tctacaggag gcatacacga
     2821 agccgcagcc cttacaaact tgaataatga ggtcgacttc atcaaaaccg acgaaagata
     2881 tatcaaaacc gatcatcagg catttgtagc attcacttat agaagaaatt tcaacatttc
     2941 gcatagactg gaagcctcat ataatcatta ccatgccgaa gatactctcg taaaattcaa
     3001 tcccatgtat ggaccatgca atgttagcta tctttcggta tcttacctgt tcaaggccga
     3061 ttatcgcgat tacaagcact atcctctcaa aggatggtat atggatttgg aagtcgataa
     3121 gtacggtctg ggaatcctcg acgatggaaa tatagattta tggaagtttt ctgccaatta
     3181 tagaatcttt tcgccgattt ccaacaaatt atatttggct tttggcttgt attcgagctt
     3241 tcaacccgac atattgcccc cttacccttt gaatgcaggt ttaggttatg gacgatattt
     3301 tgtaagagga tacgaatatt atgtaatcga aggctattgg cagtctctgg caaaaaccac
     3361 cttgaaatac acactcatac ctcaaaagat tactcgattg catggaatca aatccgaaaa
     3421 attttcgaag ttgcattatg cattttactt gaatctattc agcgacctgg ggtattccaa
     3481 accatggtca gaacttacac ctactcgact taataatcaa ttgctttaca gtatagggat
     3541 aggactcgat tttgtaacct attacgacaa ggtgcttcgg ctggaatgca ccttgaatca
     3601 caaaggagaa accggaattt acctccattt tatggcagct atttaaagaa atttcaatga
     3661 tataatgaac tgtatgtcct tagtatgttt aaaaatcatc tatacgaatt tctcaccaaa
     3721 acaaaaacca atatttcaaa tcaattccat aaaagccttc aaacaaccat tgacaaaaaa
     3781 tgcactacgg aagacgctgg agaaagttta ttgaataatc ggaccattca aaagaaatac
     3841 ttgttacctt agaggaaaaa tcaattaatg gtagaattaa caatttttgt ttttttatgg
     3901 cattcatact ctcagtaaat ggaataagcc ctgtgatagg caagaatgtt tttttggctc
     3961 ctaatgccac tattgcaggc gatgtagtaa taggagactg ttgcactgta tggttcaatg
     4021 ccgtggtaag aggcgatgtt catagcattc gaatcggcaa taacgtgaat atacaggatg
     4081 gagccattat tcattgcact tataaaaaat cacccgtcaa tatcgggaat aatgtatcta
     4141 ttgctcacgg agctattgtt catggctgta cgattcacga caatgtcctg attgggatgg
     4201 gagccatagt gatggatgat gcgatcattg aaagcaacag catagttgct gccggagctg
     4261 tgatttcgaa aaatgccatc gtaaaatcag ggacggtgtg gggcggtgtg cctgcccgcc
     4321 agatcaagga aatagatact gaattgctcg agggcgatgt attgcgaatc gccaatgcct
     4381 ataatatgta tgcgagttgg tatgccgaaa attgggaaac cgacgggaaa cctcgaaatc
     4441 gtatttgaaa tttttagata gccaacagag ggtcctgttg aggaatttgc atccataagt
     4501 ttcatcgcta aagaacgagc ttatgtactt atgtaatggc gacagccttt ttacgggtca
     4561 tatttataat actttcttca gtttactatt atttcacgaa ataggtaatc aggcaggatt
     4621 ggttctataa gtcagaaaat tctctgcacg acattgattt tgagctctgc ctgaagttat
     4681 cgggaaaaat aaacattata tttgcagcct aaggttaagg gcgaaatata aagaagatga
     4741 aaattgcact ctttggaaag aacatcattg aaaatcatca ttcatttctg gagaaattac
     4801 tccagcggtt acatgattta gattgtcata ttctgattta tgaacaatgg aagagtccga
     4861 tcgaaaccat cagaaaatca ggaatacctt atgatacttt tgccacttat cacgacttac
     4921 gatcggccga ttatctgctt tcgataggag gagatggcac tttgctcgac actttacctt
     4981 tggtgcacaa cactggtatt ccagttttag gaataaatat cgggagatta ggattccttt
     5041 cggccgtttc ccgcgatgaa atagaaaccg ttcttgattg catcatcaat aaaaagatta
     5101 tgctcgaacc acgaaccctt ctcgagataa aatccgatga ctacgatgga tacaaatttg
     5161 ctttgaatga aattagcatt ctcaaacagc ttccggcttc catgctttca atcaagactt
     5221 ttatcgacgg agaattttta aatacatact gggccgacgg tttgatagtg gctacaccga
     5281 ccggttctac agcctattcg ttgagttgcg gaggccccat actaatgccg agttccgaaa
     5341 attttgtcat tacgcctgta gccaatcata atcttacggt acggcctgtg gtcgttcctg
     5401 atagcagtaa aattgatata gaagtggata aaaaagccgg taaattttta cttgggctcg
     5461 attcgcgtat cacctccttt tctgctgggg gaaaaattat tttaaagtgt gccgatttta
     5521 aactgaatct gggacgcctt cccgatcgga atttctgtac cacgctacga gccaagctta
     5581 cctggggaca cgacatcaga aattgagcat ttgccgtgtg caatttccag aaaaatttac
     5641 gttatctctt tgtctaatga agaataccgc ttctggaaga agagcattga tgcaatgtac
     5701 ctggtgaaac gcaattttat tactttattg ctactatgga tggtagtagg cacgaatgca
     5761 caatatctcg aaagtgggat tactgcggga ggttctctct atacgggtga tctgaaccct
     5821 tcgccggctc tctcgctcac tcaacctgct gcaggtattc ttttacgata taactttact
     5881 actcgctggg tatggaaaaa tagtctgctc ttcggcaaac tcaaaggtga cgatacaaag
     5941 ggagaaaacc tctggggacg aaacctgcaa ttcgaaagca atctcacaga attaagctca
     6001 caaattgaat tcaacttcct aaattatttc accgggagtc atcgcgaaaa tttttctcct
     6061 tatctttttg ccggcttatc gctctttatg ttcaatccca aagcaccttt cgaagggaac
     6121 tggacagaac ttcaaccttt gaataccgaa ggacaggaca ctaaggcctt ccctgatcga
     6181 aaaccctatt caaaaatcag catggcaatg ccctttggtt taggaataaa atacggaata
     6241 gggaaaagat tatgcatagg acttgaaacg ggttaccgga aaacgcttac cgattatatc
     6301 gatgatgttt caacgactta ttatctcgaa ggagacaaga tcgatgctac catccccgaa
     6361 gaaatacttt ccgacccctt gcgtaatcat tcaccgggta tgcaaagagg caactcgcgc
     6421 aacaaggatt ggtatgctta tggagggatt tttatcactt acaagtttaa tctatacaaa
     6481 gcaccaccct gcaatgattt taaaaacatt cagaggtttg aatgaacatc tatgatttat
     6541 gaaatcagaa gctaaataat agtatagatc gttattacct tcttcgataa attgattatt
     6601 gtaaatttgc caaaaattca tattcaaccc ttgatgaagg gaaaaatgcc ttaaggaatg
     6661 tcagaaacat ccagattaca ggaaatcaaa gcttcactcg acatgggaaa gcttcctcga
     6721 cacattgcca ttattatgga tgggaatgga cgctgggcga ggcaaagagg catggaccgc
     6781 gtgatggggc atcagcatgc tattactgcc gttagagaaa ccgctgaagc agccgccgaa
     6841 ttgggcatac gttatctaac tctttatact ttttccaccg aaaactggaa aaggcctcaa
     6901 tgtgaaatag atgcaattat gagtttactg gttgaaacca ttcataatga aaccgatacc
     6961 ctcacaaaaa ataacatccg actcagagcc attggagatc ttgaccaatt ggaaaaatcg
     7021 acccgtcaag ctttggaaaa tgccatcgaa attacttctt cttttacacg aatggatttg
     7081 gtactcgcca tcagctatag tgcaagatgg gaaattactg aaatggtgaa gaaaattgcc
     7141 cgtgaagtag aaaatggcac tttacttccc agccaaatta ctcaggaaat tatatcggca
     7201 tacttgaatg cgcctttcct tcccgacccc gaactcttaa ttcgtaccag cggcgaatat
     7261 cgcatcagca atttcctcct ttggcaaatt gcttacaccg aactctattt tacacctgtt
     7321 ctttggcccg attttcgtaa agaagacctt tttttggcca tagccgatta tcaacgccgt
     7381 gaacgccgct tcggcaaaat aagcgaacaa ttgtcgtcat gaccaaagta aaattcttac
     7441 tccattaatc ggaaaaatat tctccatgaa gtccaacaga ttcattctgc ctattgcttt
     7501 taccctgttt atctatttcg tcccatttta tgttcaagca caaattacaa taggttctcc
     7561 tcaagtatcc atcgattacg acaatcccaa agaatacgag ataggaggaa ttacaataac
     7621 cggtgtgcag tatctcgatg catccgtgtt aactatgctt accggcttga agataggcga
     7681 caggataaaa gtgcccggag aagaaattac cttggcgata caaaaattat ggagacaagg
     7741 attgttcgat aatgtcagaa taattgcaca acgcatcgaa gataacctca tcttcctcga
     7801 aattcaactc gttgaacgac cccgattgag caaattcaca tttatcggaa tcaagaaagg
     7861 cgaagccgac gatctgcgcg ataagctaaa actcgcctca ggtgaagttg ttacccgcag
     7921 cagcctcgtc agagccgaag aaactattaa aaaatatttt accgataaag gatttttgaa
     7981 tgttcaggtt gatatccaag aaattagaga taccacaaaa ctcaataatg tcattctgaa
     8041 atttactatc gataaaaaac aaaaggttaa aatcaacgaa attatcattc atggaaacca
     8101 acaggttaaa acctccacca ttaaaaatgc cttgaaagaa accaaggaaa aaagcgcgtt
     8161 ccgccctttg ggaggcctcg accagctcgt atggaatacc gtaaaaaacg ccgccaagct
     8221 cgatatggca ggccttactg ataattttat cgattactac tacaccaatc tgaaattaag
     8281 aatatttaag tcgtcaaaat ttattcgcga agattttgaa accgacaagg aaaatatcat
     8341 aaagaaatac aatgaacttg gctatagaga cgccgaaatc gtgaaagatt ccatctattt
     8401 tgtagacaac aataatctga atatcgagct atggatcaat gaaggccata aatattactt
     8461 ccgcaatatt acctgggtgg gtaataccaa atattcctct gatgtattga atgcttacct
     8521 gaaaattaga aagggtgatg tttataacca gcaaatgctg caaaccaacc tttcttataa
     8581 tcctaatgga ttcgacgtga gctcgttata tctcgatgat ggttacctgt tttttaatat
     8641 tcagccagta gaagtgaatg tagaaaacga ttccatcgac cttgagatca ggatatatga
     8701 aggcaagcaa gccacattga acagggtaac cgtgaaaggt aacgatagaa ccagcgatca
     8761 tgtcattatc cgtgaactct ataccaaacc tggtcagcta tttagtcgta acgatatcat
     8821 tcgtacacaa cgtgaattag ctcaattgcg ctatttcaat cctgaaaaac tgggcgtgga
     8881 ctacactcct aatcccgagg atggaacagt tgacctggaa tataccgtag aggaaacatc
     8941 gagcgatcag atcgaacttt ctgctggctg gggttatggc cgattggttg gaacactcgg
     9001 tttgtcgttc aataactttt ccatgaggag aattttcgat aagaatgcat ggaaaccaat
     9061 accatcagga gatggacaaa aactcagtct tcgatttcaa acctatggcg ttggatatac
     9121 cagctattct gcttcattta ctgaaccctg gctaggaggg aaaaaaccta atgcctttac
     9181 cgtttcctat tatcactcca aatataccaa tggtctcaaa agtagcgaca cgctttatgc
     9241 ctcctttatt atagatggac ttggctttag tttgggtaaa cgactcgaat ggcccgacga
     9301 ttattttcag atttatcact ccatcaatct gcaacgttac agattaataa actaccccaa
     9361 tatttttact tttggaaccg gcaatggcga atataataat tttagctata ccatttcgtt
     9421 cggaagaagt agcgatgatt ctcccatatt cccgcgacga ggttcgaccg taaacctcag
     9481 cctcgaactc actcctccat atagcctctt tagcaataag gactatagca gtatgagcgc
     9541 cgaggaaaaa tataaatgga ttgaatatca taaatggaaa ttctatggta actggtactc
     9601 tcaactggta ggcaacctgg tattggcaac gaaataccgc tttggatttc tcggctattt
     9661 taacaaagaa ataggcgtta ctcctttcga aagattctat gttggtggag atgggctctc
     9721 aggatatggc agtttggatg gacgagaaat cattgctctg aggggttatt ccaacgaaag
     9781 cattactcct gaatatttcg aaaacaacgg ggtaggaggc acgattttca gccgctatgt
     9841 tttcgagttg cgttttccct tatcgctcaa ccccaactca accatctatg cccttgcttt
     9901 tatggaagga ggcaatacat ggaataaatt caccagcttc aatcctttca aggtttaccg
     9961 aagtgctggt cttggattga ggatatttct gcctatgttt ggcgttcttg gactcgacta
    10021 tgggtacgga ttcgacgagg tacccggaat ccccagcgca aatggaggtc aattccattt
    10081 ctccatcaat cagtccattg attaatgaat ataaggcata aaatttgtaa attcgtttca
    10141 aaattcaaat caaaggagga attatgataa gaaaaaggct tgctcttcta tttacggcca
    10201 taatgctggc tattggagtc agtgctcaaa aatttgccta tgtagattcg gaatatattc
    10261 ttcaaaatat ccccgaatat aatgacgccc ttgctcaaat cgatgaattt gccgcccaat
    10321 ggcagcagga actcgaagct aaatttgcag ccatcgataa gatgtataaa gattaccagg
    10381 ccgaggctgt tttattgccc gaagagatga aacgcaaacg tgaagacgaa attatgaagg
    10441 ccgaaaaagc cgcaaaagat ctgcaaaaac aacgcttcgg taaagacggc gacttattca
    10501 agaaacgtca agagctggtt aaacccattc aagataaaat ctattcggct atcgaaagaa
    10561 tcgctcaagc gaaaaattat gccatgatat tcgataaggc tggaaatgtt acagtgatgt
    10621 atgccgatgc caaatatgat atcagcgacg aagtactcga agaaatgggt tatggcttca
    10681 atacacgaaa gaataagtaa aaaccacttt gtaacacaat aaatattaca tgctatctgt
    10741 cctgaacttt tttgcgaaaa gaagaatgtt tttattaaaa taggaactcg aggaaatagt
    10801 ctgctcgaaa ttatttaatt ttggcgcata atcttaaaaa gaacattaag aatgaagaag
    10861 acttttgtta aatttttcgt acttgctaca tgtgtatttg cttttggagg agtttcggct
    10921 caaaaattcg gccacattga ttttgccaaa cttttcgtgg tgatgccagg acaggacacc
    10981 atacaggaaa agtatcgcca atatgccgat gggttgaggg tgcagatgga agccatgcaa
    11041 acggaatggg aaaccaaaat aacagattat caggcaaaat cttccagcat gactgatttg
    11101 atccgcagga caaaggaaaa ggaacttcaa gacctgcaaa gccgtatcga agcttttcag
    11161 cagcaagcca gcatcgattt acagaataaa gaaaccgaac ttacttctcc tctgatcgag
    11221 agagcaaaaa aagccattca ggacgtggct aaagaaaacg gatacactta cgtattcaat
    11281 agtgccgaag gcttaatcct tgttgctcct ccttctgacg acatcctgcc tttggtaaaa
    11341 aagaaactgg gtatcaaaga ataagaagta ttctattgct taatttaaaa actgcttccg
    11401 aatgaaatca agcggttttt ttaggaaact tagataagtt gataaacttt tcccggcaaa
    11461 cattttacga taccctgaaa ttcgagattg agcaaagcag caactacctt ggtggatggc
    11521 aattgagatt cgatgcagat tttatctata ctaaccgtgg aatctcgatt caggatttcg
    11581 acgatcttct tttcatcttc tgtataattt ataaaaagtt tcgattgtgc cgttcgagtg
    11641 ccaccctcca cttgtgccca tcccaaatca tccagcaata cttggggaga atcgagtaat
    11701 gaagcacgat tggtgcgtat caaccaattg cagccttcac tcatttgatc tccaaaacgg
    11761 cccggcaggg caaaaacatc acgattatat gaatttgcaa tatcggccgt gattaatgaa
    11821 cctcccttta ttcccgtttc tattaccagc aatgcatcgc ataaaccggc aataatacga
    11881 ttgcgtttcg ggaaattctc acgatcaggc ttggtttgag atggaaaatc cgttaatagc
    11941 ccaccgtggt cgagaatttt tttagctaat tgtcgatttt ggctgggata gattctatca
    12001 aggccatgac ctaaaatggc aatcgtcggc aaaccgtgtt ccagagccga tctatgggcg
    12061 gccgtatcta tcccgaacgc caatccgctt ataatcacca ccgaataaga tgaaagagct
    12121 tccatcagct tgtcgatgta ccatttcccg tagtccgttg cacgtcgagt gcctactata
    12181 ctcaaagaca aaggcggatt aagggattcg gtgcctttgt agtaaatcaa aatcggagca
    12241 tcttcacaat tcctcagtct caagggataa tttttatcct tgtaatagaa aacattaacc
    12301 ttgtattttt tgatgaattc gatttcttgt tgtgccctgt cgaaaggttt tgtattcaaa
    12361 atggcatttg ccgtgtattc accaattccc ggaatccgca ttaaagcaga tttcttttcc
    12421 ttgaaaaccg cttcggcact cccgcaataa ttcaccagtt ttttcccatt gatatcacct
    12481 acacctggaa taagcgtcaa ggctatttca taaagtaacg acataagctg ttgtttggta
    12541 agcgaaatta aacattataa ccaaaatcgg cctatactaa taaaaactcg aaaccatcct
    12601 ccactcttat attttcacct atatgttatc ggaatcatcg gcttatgtca aattttattt
    12661 ctaaattaat gtttccaatt tcattatttt gattcgttca aagcaaattc ttccgacttg
    12721 aatgcctacc tttgccacat tctcgaatga aagatcttgg aaaaattcag aattcggaat
    12781 gtttttcgta atccatcaat atcgacgtcg ggaattccgg aaaggaattg caaaacgttc
    12841 gagcgggaat gaagtatgaa aagggtgttg atagcgccat tgaattgggg ccttggtcat
    12901 gccacacgtt gtgttcctct gatagaggct ttcctgaaac gtaatgtcga aattatactt
    12961 gcgggcgaag gtctggtttt cgattttctt cgacatcgtt atcctttttt acccattatc
    13021 aaccttccgg gacaaagaat tacctatcaa attcgcggca gtttgatcct tgccttggca
    13081 aaatcttttc cagggttcct acggaatata gagctcgaac atcatatcat caaagatttg
    13141 attgaaaaat atcgattggg agctattatt tcggataacc gatatggttt atgggacgaa
    13201 aatattccat catttatgat cactcatcaa ataaacgtta gagggaaggg attgtggagg
    13261 attggcgaac catttgccca tgccgggatt cgtagatttt tgaatcattt tcatgaaata
    13321 tggatacccg attttcctca attccccgga ttggccggag atttatcgca cgataagagc
    13381 cctcggctaa aataccgcta tttaggtcct ttatcaagat tcgaacatgc ggcaccgatt
    13441 tacgagcatc ctcaaaatga tattatggcc attatttctg ggccagaacc tcagcgaacg
    13501 atctttgaaa atattcttac tagaaccttc gaaggtacta atctgaaaac gattattctc
    13561 agaggattgc cttcacatca agaatctatt gtaaaaatca atagaattac cctttattcg
    13621 catttgcaag acgatgattt tatcgaagtg ctgaaaaata gccgacatct tgtgtgccgt
    13681 tcggggtatt ccagtcttat ggatctaact cagctcgagc gaactgccct gctcgtccca
    13741 acccccggcc agccggaaca ggaatacctt gcaagactca gctcccactc aggatatttt
    13801 acaactatcc ctcaaaacca attccatctt gataattttc ccgatatgcc ttctttacct
    13861 tgctttcata ccataaacag ggaatatctc gataaggctt gtgaaatggt aataggcttg
    13921 ctttaataaa gggaaaaccg ccttttcgat ttttcatttc tccagagttg gattcaatac
    13981 ctttctcgca gcttcatatc gaatagataa acaagattta tacgaagcga tcgaaagttt
    14041 cggcagaaga accttgaaaa gtaatcttta tctttaggga gatgattcta ccgtttggct
    14101 ttgtggtcca caacctcctt cattaaaggc ctgaatcgta aaataatagg ttttgtttct
    14161 gtccatcccg gtgaaatagt attcgttttc accaaatacc atgatgcttc catataattt
    14221 atccggttcc agtccgaaac gaatgagata agctgttgcg tcatccttgc gattccattt
    14281 caaccatgca ttgcggggtt cggaatgacc ccttagaatt gtaaaatatt ctacctgagt
    14341 gggtggtaaa atattagcct tcccaaatat tcgcaagcca ctcaaggcaa attttcctcc
    14401 aggtatgtga agattttcga gcttgagata tcgtagttcg acgggcgatt cgagtgcgac
    14461 ataatcgtga ggcacatcag ttcgattatc agatttatcg catatcatcg tccaatcttt
    14521 tccattcagg ctgccccata ttctatactg ataatacaag cccgaagttt ttcctaaaaa
    14581 tggtacatcc tgatcggcat aatttatttg aaaggcataa accacacagg tatcgctcaa
    14641 atctgtacag atccattctc ctgcttttcc gcttttggca ctccaatagg tacgaatgtc
    14701 ctcatcaacg gcaaaattgg gataatagcc gcctaaagta ctcgatacgg tcacgggttt
    14761 ctgataattc aacaacatcc atccagtaaa ttttccattg taataatgag gaaaatctcc
    14821 aaaagctgtt tcggcataaa ggattccatc ggggtcgaag taggctggcc ataagcctat
    14881 tcttcgttcg aaattgttct ttaccccgat ggtgatggtc gaaatatgcc accattgctg
    14941 gttataatcg caaaatgtgg ctccatgccc ggctcccctt gcaaaacctc ctggtttata
    15001 actaaatgga ttgagatcct gatactgaaa tgggcccaag ggatgcaatc ccacataaac
    15061 tccatcggca taaccgctga attcagttcc aggggctgca tattgcagat agtaatatcc
    15121 gagatgtttg ttcatccatg aaccttcaat aaatggccgt aaaaatgtat tgtcgttgtg
    15181 ttctccaaac cgttcccaac cgtgtaaact atcatttaaa cgaatgagtg gttcaggttc
    15241 tgcccgtggc tttaaagttt tgcgatcgat ttcacatccg taaatcggat agaagttact
    15301 cgaaccaaaa taaagataca atttcccatc gtcatcggca aaaaaggccg gatcccaagc
    15361 tccaaccgga aaagaatcta tggctatttt ccactcatcg cccgtaggat gatgcgacat
    15421 ccagatagga aaatcgggat tgtgagtcga acctatcaat aggagactgt ctcccaaaac
    15481 aaaagctgcc ggcgcacata actcatcgta aaccctgtgc cgtggcaaaa ggaatttccg
    15541 tggaataaaa ttccatcgag ccaagtcatc gctccaccaa tatccatgtt gattggtcga
    15601 aaacagaaaa tattttccct gaaagggcac aatcaaggga tcggctgtgg tacgatgcct
    15661 tccttgctgg gtaaaatcag ggattgggca ataaacataa tcgagattca acggattgca
    15721 ataagtaagc agagtgtctt gtgaccatag tgaatgaacc atcactataa tcgaaatcat
    15781 cagcatagtg aatctataaa atatcatccg tatttttttc gagccggtat ccattatttc
    15841 catcgaaaaa aaaatattaa tgaataatgt gaaatttatt attccaggtg atttttatgt
    15901 ttcggcattt caaaattaca tgaaatttcg ctgaattcct tgcgctgaaa taagaatcaa
    15961 gaccg
//
LOCUS       DAOORU010000038        15794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4518_length_15794_cov_5.570430, whole genome shotgun sequence.
ACCESSION   DAOORU010000038 DAOORU010000000
VERSION     DAOORU010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15794
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4518_length_15794_cov_5.570430"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1263)
                     /locus_tag="PLF20_03955"
     CDS             complement(145..1263)
                     /locus_tag="PLF20_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase"
                     /protein_id="HOX80197.1"
                     /translation="MKSQIPTIVADEKIPYLDFLGEFPIHLKSYPASKITKNLLIEQN
                     AEALLIRTRTLCNEQLLDGTEIKFIGTATSGVDHIDQNYCSQAGIVVFSAPGSNSGAV
                     FQYISCAILEYAEVKRKNLKGLIMGVIGRGHVGEKIVHLGSLLGMEVLINDPPLFEKG
                     ILRVQNSLDEIMANSDILTLHVPYTTTGFYPTHYLIHSQNILKMKPDACLINTSRGSI
                     VDESALYRFALAHEKFSYILDVWENEPEIELRLMERALIATPHIAGYSLDGKRNASQQ
                     IFTRLAEFYHWDKQPVLPEIGPPPHARLLVEHLIEAVRATYDIFQDDEALRKNSGLFE
                     QLRSSYNFRHEFPHFEVKIKNESEKNIAMRLGFKEWNH"
     gene            1252..1533
                     /locus_tag="PLF20_03960"
     CDS             1252..1533
                     /locus_tag="PLF20_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80198.1"
                     /translation="MAFHIQNNIERESKGMNKNYFFSVFGKSLSPLFEEEFSLGWGKI
                     QHKGFVVLLNPIKLREPMKTQLKEGMMCQKIKIIEKMQRHPCYEQKNLL"
     gene            complement(1533..1605)
                     /locus_tag="PLF20_03965"
     tRNA            complement(1533..1605)
                     /locus_tag="PLF20_03965"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1570..1572),aa:Gly,seq:ccc)
     gene            1783..2364
                     /locus_tag="PLF20_03970"
     CDS             1783..2364
                     /locus_tag="PLF20_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80199.1"
                     /translation="MDFTKARDFILNELRTGLRPKFHYHSTDRTLDVYDSAMCLARLE
                     NLDALESDLLKTATILNDAGMMNTYKGHEETSAELARLWLPRFGYTKQKTEWICEMIM
                     ASKTPHKTSTLSSKILCDADLDYLGRDDFFMILHKLRIEWIQLNLINLNLKEWHHLQL
                     EFLSTHQYFSNAARESRQKGKEENIWQISNLLS"
     gene            2510..3934
                     /locus_tag="PLF20_03975"
     CDS             2510..3934
                     /locus_tag="PLF20_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80200.1"
                     /translation="MTGAVVIRLIAAYFSVGYVFAFEHFDVIEPTYNLHPGFDVQKPY
                     PFYFMLHQGLFSVMSSMNILTPRTQMLIIRIIHAIFSIITVLFGYSLAKASGNKRGAY
                     LAFALLAFAWIIPFISVRNYAFNLAMPFIVMAVWLARVSLQKYYYLSFLGGLAGGLSI
                     AIYPHAAVYWLIFILFTLFIKKPIRFLLLALGTWISLLLSLWYIHQVPISSPLTVWTY
                     LWQNLYPEQIALNTSGWIGPLILALVLGPPLGFFVLFGFLRLRKETIRIAAPALVCFL
                     IGTFLQNRYHQLFIAFPFIVLTGSHGWTDFIEKSKSWSSRYRSLYYAMLGYFWTANSI
                     LLIWISTVAPHQPLIDALEYIGKNPKVNVIVAVEPTSDFFEPVPVYYAAKSIQVKYIS
                     NAKDIDSLRNHIRMHSRDSLPGYVLFLGEKNLKQRIKTVSEIMPSLSVNAIFKAGFTE
                     RNIRRILHQPTSGSVIVYKNDYFY"
     gene            3950..6010
                     /gene="ligA"
                     /locus_tag="PLF20_03980"
     CDS             3950..6010
                     /gene="ligA"
                     /locus_tag="PLF20_03980"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008157930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOX80201.1"
                     /translation="MELEHARTRIEELTEMIEQHNYNYYVLNAPTISDYEFDKLMAEL
                     IKLEKQFPQLASPYSPTQRVGGEITKEFQTVRHRTPMLSLSNTYTDTEIRDFDQRVRK
                     TIGNEVEYVCELKFDGVAISLIYEDGKLIRGVTRGDGTQGDDVTANVKTIRSIPLRLK
                     GDFPSQLEMRGEIYMTHAEFNRLNQEREEIGEIPFVNPRNAAAGSLKLQDSTEVAHRH
                     LECFVYYVLSDSLKYDNHYDSLMAACNWGFRISPYISRCKNIDEIFDFIEEWNKGREH
                     LPFDIDGVVIKVNSFRHQQILGTTAKSPRWAIAFKFQPESVSTRLLNISFQVGRTGAI
                     TPVAELEPVFLAGTTVKRATLHNADIIASLDIRVGDYVFVEKGGEIIPKITGVDFSKR
                     PPSSQPFTFIEYCPECGTHLRKNPGEATWYCPNETGCPPQIKGRLEHFISRKAMNIES
                     LGEGRIEILYENRLVRKISDFYKLTYNQLIGLKKVYPATDKTPARVVSFREKTVENIL
                     KGIEQSKSVPFPRVLYALGIRYVGETVARKLALHYHSIDRLMQASFEELILVDEIGEK
                     IAESIVKTLHQPEMKEIIEELKKAGVQMSLSVEEKQTQGNILAGKTFVVSGDFGSAER
                     RREIEQMVVNNGGKLGTSVSSKTSFIVAGSQMGPSKKQKAIELGIPLLNEQEFLDLLG
                     KSHP"
     gene            complement(6073..8223)
                     /locus_tag="PLF20_03985"
     CDS             complement(6073..8223)
                     /locus_tag="PLF20_03985"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOX80202.1"
                     /translation="MNIEDFRKTHRLLKEYVHEWHPIYRGYNNRTLYINVDTLEFKEK
                     PVNEEMKEKFTGGKGFGLKLLWEGTLPTTKWNDPENEVVISPGPIAGITQYSGTGKSL
                     VVSISPTNFVMDSNVGGFFGPFLKFCGFDALELQGISKQDIIIYFDGINHKIEVFDAN
                     GLEFNSHLLAAQLHEIFASDENDKFNVSVVSTGQAAEHSLIGMLNFSFYDKRRKEVRL
                     KQAGRGGIGTVFRHKNIRALVAKVPGISGNLNNVVDLEAIQECGRRFNREMRELDDKQ
                     NRMRKVGTAHLMEIMNDYDLLPTHNFKFGSHPEAYKIDSNVWTRKFTQNIPDGCWVGC
                     NMACAKGVDGFELQTGPYKGTKVLVDGPEYENAAGLGSNSGIFDPDIIIELNFYCDTY
                     GVCTISWGTLTAFLMECWENGILNKKRTGGLDLSWGNGLDALELLHQVARSEGFGKIA
                     GLGIAGCKRYFVEHFGADQQFLDDIGMINKNLEYSQYVSKESLAQQGGYAMTNKGPQH
                     DEAWLIFMDMVNNQIPTFTDKAEALHYFPMFRTWFGLNGLCKLPWNDVEPENNHLTDE
                     PAKVPEHVDNYVTIFNAVTGRKIDKHELIRMSERVYNYQRIFNIRRGYGIRKYDAQPY
                     RAAGPVTIEEYESRQERYDNQLREILHLDPNVMTTKEKMAALRKYREAQYEKLLDAVY
                     ERRGWNKNGVPTIEHLKKINMDLPELIEVITPLQ"
     gene            8531..8710
                     /locus_tag="PLF20_03990"
     CDS             8531..8710
                     /locus_tag="PLF20_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOX80203.1"
                     /translation="MDTEEKLIETLTRAGKPMRSGEIAQATKLDKKEVEKAIKVLVKQ
                     GKLFSPVRCFYDIKK"
     gene            complement(8720..9211)
                     /locus_tag="PLF20_03995"
     CDS             complement(8720..9211)
                     /locus_tag="PLF20_03995"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOX80204.1"
                     /translation="MNRLNLLFFIFLVLSIPSYSQHRVQIYHPEANALADLQQAIQKA
                     AKENKHVFVQIGGNWCPWCIKFHSFIHENAKVDSLLKADYIICLVNYSKENKNQEVLK
                     LLEYPQRFGFPVFVILDKNGQRIHTQDSGLLESGEGYDEGKVIHFLRNWSPRAFDPSL
                     YTL"
     gene            9331..9933
                     /gene="thiF"
                     /locus_tag="PLF20_04000"
     CDS             9331..9933
                     /gene="thiF"
                     /locus_tag="PLF20_04000"
                     /inference="COORDINATES: protein motif:HMM:NF006395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOX80205.1"
                     /translation="MDVSQQISKYLKKARVGIAGVGGLGSNAAVALARVGVGTLIVAD
                     FDVIEIGNLNRQYYFFHQVGLPKVMALKENIHQIDAQIRVVSHHVKLNQQNSILIFRD
                     CDVLIEALDNAQMKQMFIETALSEWPYRPLIVGSGLAGWGKNEIIKVQQYDNLAICGD
                     EMFEVSDSLPPLAPRVAVVANLQANAALEFLLKDFKFKNE"
     gene            9945..10148
                     /gene="thiS"
                     /locus_tag="PLF20_04005"
     CDS             9945..10148
                     /gene="thiS"
                     /locus_tag="PLF20_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOX80206.1"
                     /translation="MKIILNHKEETIDADTLTLAELIRLKKFTFRILVTKVNGRVVKA
                     EDRDNIWIKDGDNVEVIHLISGG"
     gene            complement(10219..10884)
                     /locus_tag="PLF20_04010"
     CDS             complement(10219..10884)
                     /locus_tag="PLF20_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOX80207.1"
                     /translation="MKLKLLAMFLSCQLSVFSQMESAKFYERIDSVLAYWPEEKVANC
                     NTAIDSDELSDTEKRMVLYINLARIDGKRFAKEIIPLYIHYNPYVNMESEYFRSLLRE
                     LVLLEELPPFLVHPLLNQLAKEKTLSLKNESYISHSGADERFDEIFKAGGLSAGENIQ
                     AGDDDPFIVVMSLLIDEGVADYGHRRNLLDRSFTHIGLNLGSQKIFDYITVMEFAGFP
                     ASD"
     gene            11037..11222
                     /locus_tag="PLF20_04015"
     CDS             11037..11222
                     /locus_tag="PLF20_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80208.1"
                     /translation="MFEKLDLLYQNYFPYEQLIVGKQLIKIYSRRHAVGLSFKLVIAC
                     RLMPFDKCFYDLSFQLV"
     gene            complement(11532..12116)
                     /locus_tag="PLF20_04020"
     CDS             complement(11532..12116)
                     /locus_tag="PLF20_04020"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOX80209.1"
                     /translation="MNESDKILLPPSHMIYPLPAVLVSCGTLQHPNAITIAWTGTICS
                     KPPMCYISVRPERYSHHLIKETGDFVINLCDESMLEMLDWCGIHSGRKVDKFKEMNLS
                     ALRAVKVEAPLVKEALINIECKVKEIKTLGSHDMFIAEIVQVHSDRSLVDEKGKFQPL
                     KAKWFVYNHDLYQTVGPEIGEYGFTHHLRKNSGE"
     gene            12219..13748
                     /gene="guaA"
                     /locus_tag="PLF20_04025"
     CDS             12219..13748
                     /gene="guaA"
                     /locus_tag="PLF20_04025"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOX80210.1"
                     /translation="MHETIVILDFGSQYTQLIARRIRELNVYCEIFPFHIIPKLNPSI
                     KGVILSGSPYSVNQSGAPSPDLSGIRGKVPILGICYSAQYISKISGGCVEVCDTREYG
                     RANLHYINPNNPLMRGLSPNTQVWMSHGDTILEVPDNFEIIASTADVKVAGFEIRNEK
                     IWGIQFHPEVIHTVEGKTLLKNFVVDICHCSQDWTPASFVDETVQYLKNLIGNDEVIM
                     GLSGGVDSTVAGMLLHHAIGNHLHCIFIDNGLLRKNEFGHVLESYKRMHLNVKGVDAS
                     GRFLRALEGISDPEAKRKIIGRTFIDTFDEEAAKLEGIKWLGQGTIYPDVIESVSVNG
                     PSATIKSHHNVGGLPDMMNLKIVEPLRNLFKDEVRLVGRELGIDKEFLSRHPFPGPGL
                     GIRVIGEITRERLEILREADAIFVDALKQAGLYDKVWQAFVVLLPVKAVGVMGDERTY
                     ENVLAIRSVQSTDGMTADWTHLPYDFLAQVSSSLINHVNGINRVVYDISSKPPATIEW
                     E"
     gene            13760..>15794
                     /locus_tag="PLF20_04030"
     CDS             13760..>15794
                     /locus_tag="PLF20_04030"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOX80211.1"
                     /translation="MKQRFFIIAFLLGFFFITSKAQEPAEVVVSQQKITENGMTFWLH
                     TVEAGQTLYRISLAYKVPISDILQYNPEAAKVLKTGQQLKIPATPSEISPYTNIDFIY
                     HVTKAGETLQSISNIYSVPIETIQKLNPGIGKDKLPADQVIKIPLDYSALLKNQPIPD
                     TSRRTSKGGFLTYTVKEKETLYAISRKFGVTIADIIEWNPQVKEGLKAGEEILIGKPG
                     IKPQERGFIEYKIQKHESLYGIARKNRISIDSLKMFNQGLTENIKEGQIILIPKRRAA
                     SYITHVAEPKENLQKIADKYEVGEEELKKANPHIDKKIEENAPIKIPIQSENKKEHEM
                     AQLPAETENIASTSLCNPDYKFKHEEFKIAIIVPFMLNTPLNVRSQSSAYGKKPGESP
                     LFKYLPFYEGALLALDSLKKIGFNAKVYVYDLGNDMSGTHRLLQKPEMKSMDLIISLA
                     FSKNFELIADFAKRHKIPTINAISKRDEILSNNEYIIKPYPSEDFQAEAVAQFLSSRP
                     GKQNIIILRNNKNLYTNTIDKLLTMLDQHLQEGHFTKGSRIHFLNDSVTKVKSAIKPG
                     YSNYLIVLSENEAFNINLLRNLRTLNDTLRFSLIGMPSWANMKNAEISSLIQNNVHFL
                     SPFMVDYYDEKVQDFLYKYRDRYLAEPNELAFTGFDITWFFCNALMNYGKDFRE"
BASE COUNT         4797 a         3155 c         3080 g         4762 t
ORIGIN      
        1 aggatggaaa gtacccgaag gagaatacat ctgggacgat catctaaaaa ttccttttaa
       61 aatgacaatt cccggtgaga tacatttaaa acatgaaaaa aacgccaaag cttttcttca
      121 ggttttgttc gatgaatacc tgaatcaatg attccattcc ttgaaaccaa gcctcatggc
      181 tatatttttc tctgactcgt tcttgatttt tacctcgaaa tgaggaaatt catgacgaaa
      241 attataagaa ctcctcaact gttcaaataa gccggagttt tttctcaagg cctcatcatc
      301 ctgaaaaata tcgtatgtcg ctctaacggc ttcgataaga tgttctacca gcagcctggc
      361 atgaggagga ggtccaattt ctggcaaaac aggctgctta tcccaatgat aaaactcagc
      421 aagccgtgta aagatttgtt gtgaggcatt gcgtttccca tcgagactat aacccgcaat
      481 atgtggagtg gcaatcaaag ctcgttccat taagcgcaat tcaatttcgg gttcattttc
      541 ccaaacatcc agtatgtagc taaatttttc atgagccaat gcgaatcgat aaagagccga
      601 ttcatccact atgcttcctc tagaagtgtt gatgaggcaa gcatctggtt tcattttcag
      661 aatattctgg gaatggatga gataatgggt tggataaaaa ccagtggttg tgtaaggcac
      721 gtgtaaggta agaatgtcgg aatttgccat gatttcatcc aaagaattct gaaccctcag
      781 aatgcctttc tcgaagaggg gaggatcgtt aataagaacc tccattccaa gcaaagagcc
      841 caaatgaacg attttttcgc ctacatgtcc tctgccgata actcccatga ttaatccctt
      901 caaatttttc ctttttacct cggcatattc aaggatggcg cagcttatat attgaaaaac
      961 agcacccgaa ttacttccgg gtgcactaaa aaccacgatt ccagcctgtg aacaataatt
     1021 ctggtcgata tgatcgactc cactggtagc cgtacctatg aattttatct cagtcccatc
     1081 gagaagctgt tcgttgcata gcgttcgtgt gcgaataaga agagcttccg cattctgttc
     1141 aatcaataga ttttttgtaa tcttagaggc aggataagac ttcagatgga tgggaaattc
     1201 acccagaaaa tccaaataag ggattttttc atctgcaacg atggttggga tttggctttt
     1261 catattcaaa acaatataga gagagagtcg aaaggtatga acaaaaatta ttttttctca
     1321 gttttcggaa aatcactttc cccacttttc gaagaagaat tttccttagg ttggggtaaa
     1381 atacaacaca aaggctttgt ggttttatta aatcctataa aacttcgaga gcctatgaag
     1441 acacaattaa aagaaggaat gatgtgccaa aaaattaaaa ttatcgagaa aatgcaaagg
     1501 catccgtgct atgagcagaa aaatttgcta tgagcggaaa acgggattcg aacccgcgac
     1561 cctcagcttg ggaagctgat gctctgccaa ctgagctact tccgcatttt tttgttccgc
     1621 aaaattatat ctttcgtcat ttttatgcaa atttgcctaa aaacgaatta taaactattt
     1681 ttactccgtg aagttgaatt atcagaattc ggaaaagaaa gtcattcgca agatcgaaca
     1741 tcattttgtg attcattaaa tttcaacatt atagaaatca ttatggattt tacgaaagca
     1801 cgcgatttta ttctcaacga gctacgaacc ggtctgaggc ctaaatttca ttatcatagc
     1861 accgatcgca ccctcgatgt ttatgattcg gccatgtgtt tagcaaggct cgaaaatctc
     1921 gatgccttgg aaagcgattt attgaaaaca gccaccatat tgaatgatgc tggaatgatg
     1981 aacacctata aaggtcatga ggaaacttca gctgaactcg ccaggttatg gttgcctcga
     2041 ttcggttata caaaacaaaa aaccgaatgg atttgcgaaa tgattatggc tagcaaaaca
     2101 cctcacaaaa cctctacttt gtcttccaaa atcctttgcg atgccgatct cgattacctt
     2161 ggccgtgatg atttcttcat gattttgcat aaattacgga tagaatggat tcaattgaac
     2221 ctcatcaatc ttaacctgaa ggaatggcat catcttcaat tagagttttt atccactcat
     2281 caatatttta gcaacgcagc gcgcgaatcc cggcaaaagg gcaaagaaga aaatatctgg
     2341 caaatcagta atcttttgtc gtaattttcc ataaaaatca gtttatcagt aagttgacta
     2401 caacgacata acgaataact ttaaattttt taccaccata ctacccattg taagattttt
     2461 tcgtatcaag atccacagtt actttcgtcg caatcctttt ggatttattt tgacgggagc
     2521 ggtggttatc aggctgatag cagcttattt ctccgtaggt tatgtgtttg cctttgaaca
     2581 cttcgacgtg atagaaccga cttataattt gcatccgggt tttgatgtac aaaaacctta
     2641 tcctttttac tttatgctgc atcaaggttt attttcggtg atgagttcga tgaatattct
     2701 tacaccccgg actcaaatgc tcatcattag aatcattcat gcaatttttt ctatcattac
     2761 cgtgttgttt ggatacagcc tggcaaaggc atcgggtaac aaaagaggag catatctggc
     2821 ttttgctctt ttggccttcg cctggattat tccttttatc tcggtaagaa attatgcatt
     2881 taaccttgct atgcctttta tcgtcatggc tgtctggttg gctcgggttt ctttacaaaa
     2941 atattactat ttgtcgtttt tggggggatt ggccggcgga ttgagtatag ccatctatcc
     3001 acatgcagca gtttattggc tcatttttat cttattcact ctgtttatta aaaaacccat
     3061 tcgtttttta cttcttgccc taggtacatg gatctcccta ttgcttagct tatggtatat
     3121 tcaccaggtt cctataagtt ctcctctaac ggtttggact tatttatggc aaaatcttta
     3181 ccccgaacaa attgcgttga atacttccgg atggattgga ccacttattc ttgcccttgt
     3241 tttgggtcct cctttgggtt tttttgtatt attcggattt ttacgcctca gaaaggaaac
     3301 tattcgtatc gctgctcctg ctttggtttg ttttctgata ggcacctttc ttcagaatcg
     3361 ataccatcaa ctttttattg ccttcccatt tattgttcta accggcagtc atggctggac
     3421 tgattttatc gaaaagagca aatcatggtc gagccgttac cgatccttat actatgccat
     3481 gctaggatat ttctggacag caaacagtat tttattgatc tggatttcga cggttgcacc
     3541 tcatcaacct cttatcgatg ccttagaata tattggaaaa aatccaaaag tgaatgttat
     3601 tgtcgccgta gaaccaacat ctgatttctt cgaacctgtt ccagtgtatt atgccgctaa
     3661 gtctattcaa gtaaaatata tttcgaatgc caaagacatt gattcgttga gaaatcacat
     3721 tcgaatgcat tcgagagatt ctttgcctgg ctatgttttg tttttaggag aaaaaaattt
     3781 gaaacaaaga attaaaaccg tttctgaaat aatgccttca ttgagtgtta atgcaatttt
     3841 caaggcagga tttaccgaac gaaatattcg ccgaatttta catcaaccta cttcgggaag
     3901 cgttatcgtt tataagaatg attattttta ttaaaaattc gaaattatca tggaactcga
     3961 acacgcacga actcgtatcg aagagcttac agagatgatc gaacagcata actacaatta
     4021 ttatgtattg aatgctccaa ccatcagtga ttatgagttt gacaagctaa tggccgaatt
     4081 gatcaaactc gagaagcaat tccctcaatt ggcctctccc tattcgccca cacaaagagt
     4141 gggaggagag ataaccaagg aatttcaaac agtaaggcat cgcacaccta tgctgtcgct
     4201 ttccaataca tataccgata ctgaaattag ggattttgac caaagagtta gaaaaacgat
     4261 aggcaatgaa gtggaatatg tttgcgagtt aaaatttgat ggagtggcaa tcagtttgat
     4321 atatgaagat gggaaactga taaggggagt aacccgtggt gatggtactc agggagatga
     4381 tgtaactgca aacgtaaaaa ccattcgcag cattcctttg cgattaaaag gggattttcc
     4441 ttcacaactc gaaatgcgtg gcgagatcta tatgacacat gccgaattca accgtttgaa
     4501 tcaggaaagg gaagaaatag gagaaatccc gtttgtcaac ccgagaaatg cagctgcagg
     4561 cagtttgaaa ctgcaggatt cgactgaagt tgcccaccgc catctcgaat gctttgtcta
     4621 ttatgtctta tcggattctc tgaaatatga taatcattac gacagcctga tggcggcttg
     4681 caattgggga tttcgtattt ctccctatat atcccgttgc aaaaatatcg atgaaatatt
     4741 cgattttatc gaggaatgga acaaaggtcg tgaacacttg cctttcgata tcgatggagt
     4801 tgtgattaag gtgaattctt ttcggcatca gcaaattctc ggtactacag cgaaatcacc
     4861 acgttgggct attgctttca aatttcagcc agaaagcgtt tcgacacgtt tgttgaatat
     4921 tagttttcaa gtgggccgga caggtgctat tactccagta gctgaactcg aaccggtttt
     4981 tttggccggt actacggtaa aacgagctac acttcacaat gccgatatca ttgcttcact
     5041 cgatattcga gtgggtgatt atgtctttgt cgaaaaagga ggtgaaatca tccctaaaat
     5101 caccggagta gatttttcga aacgcccacc ctcctcccaa ccttttactt tcatcgaata
     5161 ctgcccggaa tgcggaacac atttgaggaa aaatcctggt gaagctacat ggtattgccc
     5221 taatgaaacg ggttgtcctc cgcaaataaa aggtcgtctc gaacatttta tcagtcgcaa
     5281 ggctatgaat atcgaatccc ttggcgaagg acgtatcgaa attctttatg aaaatcgact
     5341 ggtacgtaag ataagcgatt tttacaaact gacttacaat caattgatcg gtttaaagaa
     5401 agtttatcct gctaccgata aaacccctgc ccgcgtggtc tctttcaggg aaaaaaccgt
     5461 cgaaaatatt ctgaaaggaa tagaacaatc caaatcggtt cctttcccga gagtgttata
     5521 tgcccttggt attcgctatg taggcgaaac cgtagctcga aaactggctc ttcattatca
     5581 ttctatcgac cgtctcatgc aggccagttt cgaagaatta attcttgtag atgaaatagg
     5641 agagaaaatt gccgaaagta tagttaaaac tcttcaccag cccgaaatga aagaaattat
     5701 cgaggaactg aagaaagctg gtgtgcagat gagtttatcg gtcgaagaaa aacaaaccca
     5761 gggaaatata ctggctggaa aaacttttgt agtgagtggt gatttcggtt ctgccgaacg
     5821 acgcagagag atcgaacaaa tggtagtaaa taatggaggt aaattgggca caagtgtaag
     5881 ttcgaaaacc agtttcatag tggcaggcag ccaaatgggc ccttctaaga agcagaaagc
     5941 cattgagttg ggaatccccc ttctcaacga acaggaattt cttgatttgc ttgggaaatc
     6001 tcatccttaa ttcatcctta tcccattcac ttcaattcct atacaataat tcctctgtag
     6061 aaagaatgtg ttttattgta aaggagttat tacttcgatg agttcgggta gatccatgtt
     6121 gatttttttt agatgttcga tggtaggcac gccattcttg ttccaacctc tccgttcata
     6181 cactgcatcg agcagctttt cgtattgtgc ttcacgatat ttgcgaagag cggccatttt
     6241 ttcttttgta gtcattacgt taggatcgag atgaagtatt tcacgaagtt gattatcata
     6301 acgttcttgt cggctttcgt attcttcgat ggttactggc cctgcggcgc gataaggttg
     6361 agcatcgtat ttacggattc cataacctcg ccgaatatta aaaatccgtt gatagttata
     6421 cactcgctct gacatgcgga tgagctcgtg tttatcgatt ttgcgaccag taacggcatt
     6481 gaagattgtt acataattat ctacatgctc tggaaccttg gcaggttcgt ctgttagatg
     6541 gttgttttcg ggttccacat cattccatgg aagtttgcat aaaccgttta ggccaaacca
     6601 tgtcctgaac ataggaaaat agtgtaaggc ttcggccttg tcggtgaatg tagggatttg
     6661 attattaacc atatccatga aaattagcca ggcctcatca tgttgggggc ctttattggt
     6721 catagcatag cctccttgct gagctagaga ttctttcgag acatattgag aatattcgag
     6781 gtttttgtta atcatgccaa tatcgtcgag gaattgttga tcggcaccaa aatgctctac
     6841 aaagtagcgt ttgcagcctg ctatgcccaa tcctgcaatc tttccaaatc cttcactgcg
     6901 agctacttga tgaagcagtt caagggcgtc gagaccgttc ccccatgata aatccaatcc
     6961 tcccgtacgt tttttgttga ggataccatt ttcccagcat tccatcaaaa aagccgttaa
     7021 ggtgccccag ctaatcgtac aaacaccata agtatcacaa taaaaattca attcgataat
     7081 aatgtcaggg tcgaaaatac ctgagttgga acccaaacct gcggcatttt catattcagg
     7141 tccatccaca agaactttgg tacccttgta aggtccggtt tgtagttcaa aaccatctac
     7201 tccctttgcg caggccatat tacagcctac ccagcatcca tctggaatat tttgagtaaa
     7261 ttttctcgtc catacgttcg aatcgatctt ataggcctca gggtgcgaac cgaacttgaa
     7321 attatgcgtg ggaagcagat cataatcatt cattatctcc ataaggtggg cagttcctac
     7381 tttgcgcatg cggttttgct tgtcgtccaa ctcgcgcatt tcacgattga atcttctgcc
     7441 acattcctga atagcctcta aatcgactac attgttcaaa tttccactga taccaggcac
     7501 tttcgccacc aaagccctga tgttcttatg gcgaaaaaca gttcctatgc cacctctgcc
     7561 agcttgcttc aaacgtactt cctttcgtct tttatcgtaa aaactaaaat tcagcatgcc
     7621 aattaaactg tgttcggccg cctgacctgt cgaaacaacc gaaacgttga atttatcgtt
     7681 ctcatcactg gcaaaaattt catgcaactg agctgccaat aaatgactgt tgaactcgag
     7741 accattggca tcaaagactt caattttatg attgatgcca tcaaaataaa tgataatgtc
     7801 ttgttttgaa atcccttgaa gctctaaagc atcgaagcca caaaatttga gaaagggacc
     7861 gaaaaaacct cccacattgc tgtccattac aaaattcgta ggcgaaatgc ttaccaccag
     7921 tgatttcccc gtacctgagt actgtgtaat acccgcaatt gggccaggtg aaataacgac
     7981 ttcattttca ggatcattcc attttgtggt aggtaaagtg ccttcccata agagttttaa
     8041 accaaatccc ttaccaccgg taaatttttc tttcatctcc tcgtttacag gcttttcctt
     8101 gaattcgaga gtatctacat tgatatacaa agtgcgattg ttgtaacctc tataaatggg
     8161 atgccattcg tgaacatatt ccttcaataa tctgtgcgtt ttccgaaaat cttcaatatt
     8221 cataactcga tattttttta aagaactttt atcctaaaaa ttttacaaat atataagcaa
     8281 ttttcctgtt ttaaaataga ataattaatt aaagcattta tttttttgaa aaattataat
     8341 ttaaaatatg gcatagtgaa ctaaaacagt tagttataaa tttcgatttg aaaaattttt
     8401 caacttgctt tttttaattt tatcattgaa aaacctgatt cgacttaaca tattttattt
     8461 cgaaagaact ttcagcttta aaaccataaa aacttatacc tttgcattaa acaataaata
     8521 agaagactat atggatacag aggaaaaact cattgaaacc ttaactcgag cgggaaaacc
     8581 catgcgttct ggtgaaattg cccaggctac caaactcgat aaaaaagaag tagaaaaggc
     8641 catcaaggta ttggtgaagc aaggcaagct attctctccg gttcgttgct tctatgatat
     8701 aaagaaatag aatcatcgac taaagagtat agagagaagg atcgaatgcc cgcggcgacc
     8761 agtttcggag aaaatggatg accttcccct catcataacc ttcacccgat tctaaaaggc
     8821 cggagtcttg cgtatgaatt ctctgaccat tcttgtcgag aatcacgaac accggaaatc
     8881 cgaaacgctg agggtattca agaagcttta atacttcctg atttttgttt tccttgctgt
     8941 aattcaccag acaaattatg taatcagcct tcagaagtga gtcaaccttg gcattttcat
     9001 gaataaagct atgaaatttt atacaccatg gacaccagtt tccaccaatt tgaacaaaaa
     9061 catgtttatt ctctttggct gccttttgaa tagcttgctg aagatcggcc agggcattgg
     9121 cctcgggatg atatatctga actctatgtt gggaataaga tgggatagac aatactaaaa
     9181 aaataaaaaa taacaggttc aatctgttca tgcgtaagat ttttcgagtt tgttattggc
     9241 cacaaaatta ggacttcggt ttgaacgaag agatgatgca aaggaatgca attcatgcaa
     9301 tgaagaattc aattgttttt tcgaatgaaa atggatgtaa gtcaacagat tagtaaatac
     9361 ttaaagaaag ccagagtcgg gattgctggc gtaggtggct taggttctaa tgctgctgta
     9421 gcattggcca gagtaggggt gggtacactc atagtagccg attttgatgt gatagaaatt
     9481 ggcaacctca accgccaata ctactttttt catcaggttg gattgccaaa ggtaatggcc
     9541 ttgaaagaaa atatccatca gatcgatgct caaattcgcg tagtttccca tcatgtgaag
     9601 ttaaatcagc agaactcgat tctcattttt cgtgattgtg atgttctcat agaagccctc
     9661 gataacgccc agatgaaaca gatgttcatt gaaactgctc ttagcgaatg gccttacagg
     9721 cctttgatcg ttggttcggg acttgctgga tggggaaaaa atgaaattat caaagttcaa
     9781 caatatgata atctggcaat ttgtggcgac gaaatgttcg aagtaagtga tagcttgcca
     9841 cctttagcac cacgtgtagc tgtggtggca aatttacaag ctaatgccgc cctcgaattt
     9901 ttactgaaag actttaagtt taaaaatgaa taagatatac aatcatgaaa ataattctca
     9961 atcataagga agaaaccatc gatgccgaca ctctgacttt agccgaactc attcggttga
    10021 aaaaattcac ctttcgcatt ttggtcacca aagtgaacgg ccgggtggtg aaagctgaag
    10081 atcgcgacaa catttggatt aaggatggag ataatgtaga agtaattcac ctgattagcg
    10141 gaggataaaa acctacctcc cttaaaatcg tttcttcaat catttcttca ggtattaact
    10201 gaaatagatc gacaaagctt aatctgaagc tggaaatcct gcaaactcca tcacggtaat
    10261 gtagtcgaaa attttctgac ttcccaggtt taagccaata tgggtgaaag acctgtccag
    10321 caaattcctg cggtgtccat aatctgctac tccttcgtct ataagaagcg acatcacgac
    10381 gatgaaggga tcatcgtcgc cggcttgaat gttttcaccc gccgacagtc ctcctgcctt
    10441 gaatatctca tcaaatcttt catcagctcc cgaatgcgaa atataagatt cattcttcaa
    10501 tgaaagcgtt ttttctttag ccagttggtt taataaagga tgaaccagaa aaggaggcaa
    10561 ttcttcgagt aatacgagtt cacgcaataa acttctgaaa tactccgatt ccatgtttac
    10621 ataaggattg taatggatat aaagtggaat gatctccttt gcaaagcgtt tcccatctat
    10681 tcgtgccaga ttgatataca acaccattcg tttttccgta tcgctcagtt cgtcgctatc
    10741 gatggcggta ttgcagttcg ctactttttc ctcaggccaa tatgccagaa cagaatctat
    10801 tctttcataa aactttgcag attccatctg gctgaaaact gaaagctggc acgaaagaaa
    10861 cattgccaat agtttgagtt tcatttcgat gaattttaat ggtcaaaaaa ataaatctgt
    10921 tacaatacaa tcattacgtt tttagaatag aaatattgct tcccaatgta aggatatttc
    10981 attcttaaaa atcaggttat tctgggttta tatcattcag tcgagtgctg atcaacatgt
    11041 ttgaaaagct ggatttactt tatcaaaatt attttcctta cgaacaactt atcgtcggta
    11101 agcaattgat aaaaatatat tcccgcaggc atgctgttgg cctttcattc aagctggtga
    11161 tagcctgcag gctgatgccc ttcgataaat gtttctacga tttgtccttt caattggttt
    11221 agtttagatg gaaattatta ttcgatgaga tgatggtaaa taatataaat tcctgaactc
    11281 caatttataa tatctataca aatgaataaa ttttttatgc attcgtcaag acctgaaggt
    11341 tttttttaga aatagttcaa attttgagaa atagttttaa gcagttttga cttttaaact
    11401 tttatgtttg cttagaaatt ctcattccaa gccattctga taaattttct ttttatgaca
    11461 gggaaaaggt gttaaaccag aaatttctga tttaaagcaa caataagcaa ttaaccgaat
    11521 attattggaa atcattcccc tgaatttttc cttaagtgat gtgtaaagcc atattcccca
    11581 atttcgggac cgacagtctg gtacaaatca tgattataaa caaaccattt ggctttcaag
    11641 ggctggaatt ttcctttttc atccacaagt gacctatctg aatgaacctg aacaatttct
    11701 gctatgaaca tatcatgaga acccagagtt tttatttctt tcaccttgca ctcaatatta
    11761 atgagagcct ctttcactaa aggagcttca accttgacgg ctcgtaaagc acttagattc
    11821 atttccttga acttatctac ttttcgtcct gaatgaatgc cacaccaatc cagcatttcc
    11881 agcatggatt catcgcaaag gttgatcaca aaatcccctg tttccttaat taaatggtga
    11941 gaatatcttt cgggtcgcac agagatataa cacattggtg gtttagagca aatcgttcct
    12001 gtccatgcaa tcgtaatcgc atttggatgt tgcaatgtac cacatgaaac caaaactgca
    12061 ggaagaggat agatcatgtg cgaaggtgga agtaagattt tatcagattc attcattgca
    12121 tcgaagtttt agatagtaca atataaaatg aattggtttt tcaaccacaa gatatgtaat
    12181 tttgcgcaaa tttcgggttt ctcaattaat tattttacat gcacgaaacc atagtcattc
    12241 tggattttgg ttcacaatat actcaattga ttgcgaggcg aatacgtgaa ttgaatgtat
    12301 attgtgaaat ttttcctttc catataattc caaagctgaa cccttctata aaaggcgtaa
    12361 ttctcagtgg tagtccatat tcggtgaatc aatctggtgc accctcaccc gatttatcgg
    12421 ggatcagagg caaggttcct attctgggaa tatgttatag tgctcaatac atctcaaaaa
    12481 tctcgggagg ctgcgtcgaa gtatgcgata ctcgtgaata tgggagggca aatcttcatt
    12541 atatcaaccc gaataatcct ttaatgcgtg gcctaagccc aaatactcag gtctggatgt
    12601 cgcatggaga cacaatactc gaagtccctg acaatttcga aatcattgca tcgacagccg
    12661 atgtaaaggt agctggtttt gaaataagga atgaaaaaat ttggggcata cagtttcatc
    12721 cagaagttat tcatacggtt gaaggcaaaa ctttgctgaa aaattttgta gttgatattt
    12781 gtcattgctc ccaagattgg actccggcgt cctttgtcga tgaaaccgta cagtatttga
    12841 agaaccttat cggcaatgat gaagtcatca tgggtttgtc gggcggagtg gattccacag
    12901 tagccggaat gcttttacat catgcaatag gcaatcatct gcattgtatt tttatcgata
    12961 atggattatt gcgaaagaat gagtttggtc atgttttgga atcctataag cggatgcatc
    13021 tcaatgttaa aggtgtagat gcttcgggaa gatttttacg agctcttgaa ggaatctccg
    13081 atcctgaggc aaaacgcaaa atcattggaa ggacttttat agacactttc gacgaagaag
    13141 ccgctaagtt agaaggaata aaatggttag gacaaggaac gatttatccc gatgtaatcg
    13201 agtcggtttc ggtgaatggt ccttcggcca ccattaagtc gcatcataac gttggaggcc
    13261 ttcccgatat gatgaatctg aaaatagtcg aacctctgcg aaaccttttt aaagatgaag
    13321 ttcgtttggt aggcagagag cttggcattg ataaagaatt tctgagtcgc catcctttcc
    13381 ccgggcctgg actgggtatt agagttatag gtgaaattac ccgtgagagg cttgagattt
    13441 tacgtgaagc cgatgccatt ttcgtcgatg cgcttaaaca agctgggctc tacgacaaag
    13501 tttggcaggc ttttgtggta ttactccccg taaaagccgt tggcgtaatg ggagatgagc
    13561 gaacatacga aaatgtgctg gctattcgtt cagtacagtc aaccgacggt atgaccgccg
    13621 actggacgca tcttccctac gattttctgg ctcaggtctc gagctctcta atcaatcacg
    13681 taaatgggat caatcgcgtg gtctacgata ttagttcgaa accccctgct accatcgagt
    13741 gggaataatt atcgaaatca tgaagcaaag atttttcatt atcgcttttc ttctgggatt
    13801 ctttttcatt acttcaaagg ctcaggaacc tgctgaggtt gtggtatctc aacagaaaat
    13861 cactgaaaat ggcatgactt tctggctgca taccgtcgaa gccggtcaaa cactttaccg
    13921 gatttcactt gcttataagg tgccaattag tgatattctt caatataacc ccgaagcagc
    13981 caaagtattg aaaacagggc aacaattgaa gattcctgcg acgccttcag aaattagccc
    14041 ttacacgaat attgatttta tatatcatgt aaccaaagca ggcgaaacct tgcaaagtat
    14101 atcgaacatt tatagcgttc ccatcgaaac catccaaaaa ttgaaccccg gtattggtaa
    14161 agacaaactt cctgcggatc aggtaatcaa aattcccctc gattattcag cattgctgaa
    14221 aaatcagcca attcccgata cgtcaaggag aacctctaaa ggaggttttc ttacctatac
    14281 agtaaaagaa aaagaaacat tatatgccat cagccggaaa tttggagtaa ctattgctga
    14341 tatcatcgaa tggaatcctc aggtgaagga aggactcaag gcaggtgagg agatattgat
    14401 tgggaagcca ggcataaagc ctcaggaaag aggattcatt gagtataaga tacagaaaca
    14461 cgaaagtctt tatggaatcg cccgtaaaaa ccgcatcagt atagattcgt tgaaaatgtt
    14521 taaccagggc cttaccgaaa atatcaaaga aggacaaatt atcctcattc ctaaacgaag
    14581 agcagcttct tatattactc atgtggccga accgaaggaa aatttacaaa aaattgccga
    14641 caaatatgaa gtaggagagg aggagcttaa aaaagccaat cctcacatcg ataaaaaaat
    14701 tgaagaaaat gctcctatca aaattcctat ccagtccgaa aacaagaaag aacatgaaat
    14761 ggctcagctt cctgccgaaa ccgaaaatat tgcatcaaca tcattgtgca atcccgatta
    14821 taaattcaaa cacgaggaat ttaaaattgc catcattgtc cctttcatgc tgaatactcc
    14881 cttgaatgtt cgtagccaga gctcagctta tggtaaaaag ccaggtgaat cacccttgtt
    14941 caagtatctg ccattttacg aaggtgctct tctggctctt gattcactca agaagatagg
    15001 ttttaatgca aaagtgtatg tgtacgatct gggaaacgat atgagtggaa cccataggtt
    15061 attgcaaaaa cccgagatga aatccatgga tttgataatt agcctggctt tcagcaagaa
    15121 tttcgagcta attgccgact ttgcaaaacg ccataaaatt cctactatca atgctatatc
    15181 taaaagagat gaaatattgt cgaacaacga atacatcatt aagccttatc catccgaaga
    15241 ttttcaggcc gaagcagttg cccaattctt aagctcgagg cctggtaaac aaaacatcat
    15301 cattctgcgc aacaataaaa atttatacac gaataccatc gataaactcc tcacaatgct
    15361 cgatcagcat ctccaagaag gccattttac caagggaagt cgaatacact ttctcaacga
    15421 tagtgtaaca aaagttaaat cagctatcaa gccgggttat tccaattatt tgatcgtact
    15481 ttcagaaaat gaagcattca atatcaatct tttacggaat ctgcgtactt tgaacgatac
    15541 cctgagattt tccctcatcg gtatgccttc gtgggccaat atgaaaaatg ctgagatttc
    15601 ttcactcatt cagaataatg tgcattttct ttctcctttt atggtagatt actacgatga
    15661 aaaggttcaa gattttttat ataaataccg tgaccgttac ttggccgaac ccaacgaact
    15721 cgcctttact ggattcgaca tcacatggtt tttttgcaat gccttgatga attatggaaa
    15781 agatttccgc gaat
//
LOCUS       DAOORU010000039        15560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4628_length_15560_cov_6.193550, whole genome shotgun sequence.
ACCESSION   DAOORU010000039 DAOORU010000000
VERSION     DAOORU010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15560
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4628_length_15560_cov_6.193550"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1412
                     /locus_tag="PLF20_04035"
     CDS             <1..1412
                     /locus_tag="PLF20_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80212.1"
                     /translation="RLAARRKSKIFGFAVNTWLFIPASLDINHSTYSKNSFYHDLKSN
                     IRLITPVYLLRDIADGENSPLEFLTTAFRKVASLPTRTLAAEYEYHIKMFLSILKSSL
                     REEIQHILNNKLPADTSYLIDEFCKNVLHITRRYRDLRYIINAPTISEDLMNYYSFGD
                     EFMSNLIEEHAFKLLSGLKESHPSFSKSWQRQLLAIIEEEIKYKKEHNYLVVEEKSPT
                     RNRELVFRLNLLKKFAESELFLTGEKKKEGIWVEQIYFSLAAGLSMVFATAVAFTFQI
                     KYGNLTMPLFVALVVSYMLKDRIKELFRYYFATRLGHRYFDHKTEISLNNIKIGVSKE
                     GMDFIPEKKVLPNVMKMRGRSPILEVNNRYDTEKIILYRKIIHLDREKLDEVSPYSTL
                     GINEILRLNVDSFMQKMDDPAVPLFIPDENKGYAIINGERIYYINMIMQMTYENETTY
                     RRYRIVLNRKGIKAIESFS"
     gene            complement(1409..2107)
                     /locus_tag="PLF20_04040"
     CDS             complement(1409..2107)
                     /locus_tag="PLF20_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018464587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX80213.1"
                     /translation="MKKLKILLAEDDKNLGTVLKAYLDAKGHDTILAIDGEEALKLFY
                     AEDFDFCILDVMMPLKDGFAVTKQIRKSNKTIPILFLTAKSQPHDIITGFNTGADDYI
                     TKPFRMEELMARIKAIMRRTTSGLQDDLTHQVYHIGNYSFDFTSQILKIGDTEFNLTA
                     KEAELLKILVENANEVIDRSIILQNVWKDDSYFTARSMDVYISRLRKYLQEDRRVQII
                     NSHKVGFRLVVPED"
     gene            complement(2160..3794)
                     /locus_tag="PLF20_04045"
     CDS             complement(2160..3794)
                     /locus_tag="PLF20_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOX80214.1"
                     /translation="MNRRILAIIIFLMGFVLVVLLFIQVYWIRNALMLREAAFTAGVE
                     EAVSNVVLSLERIESGKRLRSFKESARLYRKIDSLNYLIDKRYHELTGEDGSSAADVK
                     ARRKIIQDRKSWRTLRDLDTTSEFRENRNIQEELDSLIEPKQIQTIQTRSVNIEDDPV
                     YKRLQKQRESVVEELMSRSLIYDAALGRFDDIIEMPLEKRIQPGVVDSMLAVELQNKG
                     IKTEFEFGIYDTRRDSLIYQKTGKFTQELLNKAFAVNLFPRQPGKDKAILLVYFPYER
                     QYMVQQIWWLLLLSILLVGTFIAIFYKTISTILYQKKLSDMKNDFISNITHEFKTPVS
                     TISLACEALNDPDVVKTPAMASNYIQIITDENRRLGTMAEKILIASVLEQGKLELNMV
                     PCAMHEIITECVQKVGIQAETAGGSIQTSLQALHDMVRGDRLHLTSVVVNLLDNAIKY
                     SPIRPTIRINTYNRDQNFVFEVIDNGIGISKENQGMIFDKLYRVPTGNIHNVKGFGLG
                     LSYTKAIVEAHQGNISVSSELKKGTTFTVTLPNFEP"
     gene            3979..4539
                     /locus_tag="PLF20_04050"
     CDS             3979..4539
                     /locus_tag="PLF20_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80215.1"
                     /translation="MKRVVCFLGMALCGVILLGGLQSCDKVENLAKFDVKMDLPSLNF
                     NLVASAKNSSTQEYYKDFNVYLNLDSIQEAHSLNSFSIENGKLTKATLRIIYPAGANF
                     QFLINSRLVLFNSSNQEMQVAHTGNINANATEIELILDTADLTSLFKMKHYTGRIYYT
                     IEPSLLPAQNVTIQLNQSIQFTVHPL"
     gene            4653..6308
                     /gene="pgi"
                     /locus_tag="PLF20_04055"
     CDS             4653..6308
                     /gene="pgi"
                     /locus_tag="PLF20_04055"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOX80216.1"
                     /translation="MLPTIDPTTTKSWKKLSNYFTSIGKQSLNQLFESDNNRAQKFSL
                     QWQNLFVDYSKTHIDEQTMRLLIELARECHLAEAIEQMFRGEKINQTQKRAVLHFALR
                     NLGDDPVFLDGRDVMPEIRTVRNQMKIFSTQVRNGDWKGYTGKPIRNIVNIGIGGSDL
                     GPAMVCRGLQFYGHERLRVFFVSNVDGGHIYETLRHLNAEETLFLIVSKSFTTQETLA
                     NAKTARQWFINQGGKAEDISKHFVAISTNTPAAMEFGIDPENIFHFWDYVGGRFSLWS
                     AVGLSIALQIGYESYEELLKGAEAMDHHFRETPFENNLPILLALLSIWYTNFFDCATE
                     AILPYDQYLERFPAYLQQTIMESNGKSIDRSGLPVSYKTSPVIWGEPGTNGQHSFYQL
                     IHQGTQMIPCIFMAAAKPLTPLSEHHQLLLSNFFAQPEALMKGKTAEEVRAEMLSKGF
                     NEEEINKLLPFRVFRGNIPSISLLYQQLTPYNLGAITALFEHRTFVQGIIWNIFSFDQ
                     WGVELGKSLANNIIPELAHDDPIQTHDGSTRQLIHKVKQMRKS"
     gene            6364..7239
                     /locus_tag="PLF20_04060"
     CDS             6364..7239
                     /locus_tag="PLF20_04060"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HOX80217.1"
                     /translation="MSQEETLTRKRLRSAYITTIVSLTLVLFMLGLWGVIGLHAHKIS
                     RYVKENLGLTIYFQKNARETEILRLKKSLDTTRWVTSTHYISPEEAEQILKKELGEDF
                     IGFLGYNPLLPCLQIRLNADYTHPDSIQYIEKHLKQNPKVAQIEYQRDLINSVNKNLN
                     IISIIIAVLSFLMFLISIGLINNTIKLSVYSKRFIIRSMMLVGATRGFIRAPFVKKGL
                     WHGILSAIFAIILLSILLYAAYLKIPELKTLADFHILMIIAGAMIVTSALISVISNYI
                     AVTRFLNMNIDKLYM"
     gene            7253..7540
                     /locus_tag="PLF20_04065"
     CDS             7253..7540
                     /locus_tag="PLF20_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HOX80218.1"
                     /translation="MEKKTIVTSKKAKEEEKPQAGFAFERQNYILMVIGIVVLIIGFL
                     LMIGGGSDDPQVFNYDLFNFRRLTLSPILIIAGFIIEIFAIMWNPGKKVKK"
     gene            7537..8325
                     /locus_tag="PLF20_04070"
     CDS             7537..8325
                     /locus_tag="PLF20_04070"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOX80219.1"
                     /translation="MTWLQALIIAIVEGITEFLPISSTGHMIITEAVLGIEPSEFSKT
                     FIVNIQFGAILSVIVLYWKNFFASLDFYYKLFVAFLPAAVVGYLAGDLIDRMLGSVLV
                     VAISLVVGGIVLLFIEEWFKNTAEKGKNDVTYSSALKIGFFQCIAMIPGISRSAATII
                     GGMTQKLNRKAAAEFSFFLAVPTMFAASAYKLIENYKSINIHNIPILVFGNVIAFIIA
                     LLAIRTFINYLTRHGFKLFGWYRIFLGGLILVLMALGINLNIIG"
     gene            8331..9014
                     /gene="truB"
                     /locus_tag="PLF20_04075"
     CDS             8331..9014
                     /gene="truB"
                     /locus_tag="PLF20_04075"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOX80220.1"
                     /translation="MEEDNILLFDKPYGWTSFDVVNSVRYTLKKFTGKKHLRVGHAGT
                     LDPLATGLLIICSGKETKNIEKYQALEKEYTGTFHLGATTPTFDLESEPGNFKDISFL
                     KPLHIHEAALRFTGEQQQIPPLFSAIKIEGKRAYQYAREQEEINLDHKTIIIYQFEII
                     RTEFPEVDFRIVCSKGTYIRAIARDFGEYLGCGAYLKSLRRTRIGNFDVKDAFDIETF
                     KSSLYHKLE"
     gene            9090..10850
                     /gene="gldG"
                     /locus_tag="PLF20_04080"
     CDS             9090..10850
                     /gene="gldG"
                     /locus_tag="PLF20_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR03521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     substrate-binding protein GldG"
                     /protein_id="HOX80221.1"
                     /translation="MNAPPKDNTTSSSRKKIARQAISKLLVGILIVILVNIIGSYSFT
                     RFDLTSEKRYTLSESTKKLIKEVDDYVYFRIYLEGDFPAGFKRLRNETREMLDEMRAY
                     NKFINYEFINPNASSNPKDRQNIYRQLIEKGLNPTDLQVKDKEGESRQIIFPGAIASY
                     RGKETPVQLLTGQMGARPEEILNLSVENLEYNLANTIRKLIVSYKPKVAFIYGHGELE
                     EAFIYDISQSMAEYYTVERIKLDGQINALTEHKTDSGGISIFNKYKAIIIAKPDSAFT
                     EKDKFIIDQFLMRGGKILWFIDGVNAEMDSLQNKSSTLGLAHDINLEDMLFRYGVRIN
                     NDLVLDANCLPIMMVTGTIGNQPRFSFVPWIFSPLANPATKHPIVNNLNPIKTQFISS
                     IDTIAIKGVKKTIMLSSGQYSRKIIAPIIIDLNIARQEPDLKLFNQPYIPLAVFLDGK
                     FPSLYEHRIPANISDSPEIGFLAESKPTQMLVVSDGDVIRNQLERKNAYPLPLGYDQD
                     TRQTFGNKEFILNTLNYMLDDSKLVTIRSREIKMRLLDPLKLQNERLRWQTLNIVSPV
                     ALVLLFGLIKYSLRKRRYAH"
     gene            complement(10978..12738)
                     /locus_tag="PLF20_04085"
     CDS             complement(10978..12738)
                     /locus_tag="PLF20_04085"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80222.1"
                     /translation="MKRTKNLIDLLVVAILISVPYLVNSQNFVPYSTTLPNVAQGSCD
                     WADIDNDGYLDLAMMGANSIGQPLTKIFRNNAGVFIDIDANLIGLKNGSLRFGDYDRD
                     GDYDLLVCGSDFSSKPHSLLYKNDNGSFSQSSNTFQGIYNGMAIFVDYDNDGLLDIFL
                     SGDTLSYKGFTRIYHNLGNDNFEEVDFGFPGVISSSLSYGDYDNDGDFDLLLSGDIGG
                     AYYTKLFRNDMGLFIEIPTNIEGVGAGFSLFMDFDKDGDHDILLMGNDLTLTPIFKIY
                     RNDGNGSLQEVFVGITGLALGNISIADYNSDGYPDFATTGKAPGCGAMMSVLFYNNQI
                     GSFWPSSQNLVDLSYSYSSWADFDNDGDSDLLLMGISTSGNPETVLYSNTLNSNGFVP
                     NLPPFAPIDLSSEMNGNELILKWKPSPYNTNGKNIFYNVRVGLNPGSSEIMSTASNVL
                     SGQMLLPSFSNVTQDTFWILQGLREADYFWSVQTIDPSYSASEFAPEETFSIAYTGIA
                     PIQEKKQFFDYRVYADRIELISQNQGKWSLFSVDGIEIKNGNLGFGKSNVSLLGIARG
                     IYILRIETEDNHAALKFLKR"
     gene            complement(13078..14280)
                     /gene="pcaF"
                     /locus_tag="PLF20_04090"
     CDS             complement(13078..14280)
                     /gene="pcaF"
                     /locus_tag="PLF20_04090"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: protein motif:HMM:NF006551.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HOX80223.1"
                     /translation="MKEAFICDAIRTPIGRYGGTLASIRTDDLAVIPIKALMERNASV
                     NWEEVDDVIFGCANQAGEDNRNVARMALLLAGLSERIPGVTVNRLCGSGMEAIGQAMR
                     MIRTEDADLIIAGGVESMSRAPFVMGKASEAFSRDVQIYDTTIGWRFINKKMEQLYGV
                     DSMIETAENVAGMYHISREDQDIFAYHSQLKAHQAIQSGAMEREITPVEIVRPKSEPF
                     VFSMDEHPRLSSLEKIGTLRPVSRPGGTVTAANSSGVNDGAAALLIASENALKKYKLR
                     PMARIVGVQAAGVPPRIMGIGPVPATQKLLFKLHFSLEDFDLIELNEAFASQSLACMR
                     LLGLKEDDPRINPQGGAISLGHPLGMSGARLITTAVHQMSNNNLHRALCTMCIGVGQG
                     IASIIEKV"
     gene            complement(14295..>15560)
                     /locus_tag="PLF20_04095"
     CDS             complement(14295..>15560)
                     /locus_tag="PLF20_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX80224.1"
                     /translation="PGGISRNVIFRQPHPHYVASAHGCYVEDVNGITRIDFVNNIASL
                     VHGHAHPAIVKAVYEQLQKGTAYSMGTEAEIRHAETLCHRVPGFEKIRFMNSGTEAVM
                     SLIKASRAYTGKHKIAKAEGAYHGSYDFAEVSESASPDNWGPVEQPNSVPHVRNTPPS
                     VLDEVIVFPYNDVERTLAILDRHADEIACVLIDPVPHRIGMIRATDEFVEAVYDWTRK
                     HNTLMAFDEVVCFRINYEGAQALYRVKPDLTALGKLIGGGFPVGALTGKAEIMDVLNP
                     LQTPLRFSLSGTFSANPITMTAGRVAVEMFDKQAVDKVNHLAQEAKEQLMQAAKIAGI
                     PLSVAGAGSMMKMHFRETLPSNYRECYEDANHKKTVNTFLDFMYDHGIILVYSNSCFF
                     STVMKQEHIDKLSETALEGFKFIKPLLNV"
BASE COUNT         4623 a         3276 c         2996 g         4665 t
ORIGIN      
        1 tgcggctggc tgcaaggcgc aaatccaaaa tttttggctt tgctgtaaac acatggttat
       61 ttattcctgc atcacttgat attaatcact ctacctatag taaaaacagt ttttatcacg
      121 atttgaaatc caatatacgg ctgattactc cagtgtattt attacgagat atcgccgatg
      181 gtgaaaattc tccattggaa tttctcacga cggcttttcg aaaggtggct tctttgccta
      241 cccgcactct ggccgctgaa tatgaatatc atatcaaaat gtttctttct atcttaaaaa
      301 gctcattaag agaagaaatt caacatattc tcaacaataa acttcctgcc gatacctctt
      361 acctgataga tgaattttgt aagaatgtcc tgcatatcac tcggcgctat cgcgatttgc
      421 gttacatcat aaacgctcct accataagcg aagacctcat gaattattat tcctttggag
      481 atgaattcat gagcaactta atcgaagagc atgccttcaa attgttgtct ggcttgaaag
      541 aaagccatcc tagcttcagc aaaagctggc agaggcaatt actggcgata atagaggagg
      601 agattaaata taaaaaggaa cacaattacc ttgtagttga ggaaaaaagc cctacgcgaa
      661 atcgagagtt agttttccgt ttaaatttat tgaagaaatt tgccgaaagt gaactcttcc
      721 tcacaggaga aaaaaagaaa gagggtattt gggtcgaaca aatatatttt agcctggcgg
      781 cagggctttc gatggttttt gcaactgcgg tggcttttac ttttcaaatc aaatatggca
      841 atctgactat gcccttgttt gtggctttgg tggtgagcta catgctgaaa gacaggataa
      901 aggaactttt caggtattat tttgctacgc gcctgggaca tcgctatttc gatcataaaa
      961 ccgaaatttc cctcaacaac ataaagattg gcgtaagtaa agaaggcatg gattttattc
     1021 ccgagaaaaa ggttctgcct aacgtaatga agatgagagg tagatcacct attctggagg
     1081 ttaacaaccg ttatgatacc gaaaaaatca ttctgtaccg taaaataatt cacttagacc
     1141 gtgaaaaact cgatgaagta agcccttatt ctactttggg tatcaacgaa atattgcgat
     1201 tgaatgtaga tagtttcatg cagaagatgg atgatcctgc cgtgcctctt tttatacccg
     1261 acgaaaataa gggttatgct atcattaatg gcgagcgaat atattatatc aatatgatca
     1321 tgcaaatgac ctatgaaaat gaaactacct accgcagata ccgtatcgta ttgaatcgca
     1381 aagggatcaa agccatcgaa agtttttcct aatcttcggg aaccacgagc cgaaaaccca
     1441 ctttatggct attaatgatt tgcacacgac ggtcctcctg taaatatttc ctcaatcgtg
     1501 aaatgtatac atccatgctg cgagcagtaa aataagaatc atctttccag acattttgca
     1561 agatgatgct tctatcgata acttcattgg cattttcgac caggattttc agcagttctg
     1621 cttccttggc cgtaagattg aattcagtat ctccaatttt tagaatctga ctggtaaagt
     1681 cgaaactata gttacctata tgatatactt ggtgggtcag gtcgtcctga aggccagaag
     1741 tagttcttcg catgatggct tttatgcgtg ccatcagttc ttccatccgg aatggcttgg
     1801 taatataatc gtccgctcct gtattgaaac ccgttataat gtcgtgtggt tgagatttgg
     1861 ccgttaaaaa taaaataggg atggttttgt tggatttgcg gatttgtttt gtcacagcaa
     1921 atccatcttt caaaggcatc atcacgtcca atatgcagaa gtcgaaatcc tctgcataaa
     1981 acaatttgag ggcttcttcg ccatctatgg caaggatagt gtcatgaccc ttggcatcca
     2041 gataggcctt caatacggtt cctaaattct tgtcgtcttc ggccagcagt atctttaact
     2101 ttttcatttt cggataaaat tgacattttc aaagcaaaag tcttctaagg gtggtgtagt
     2161 tatggttcaa aattgggcaa ggttaccgta aaggtagttc cttttttcaa ttcactgcta
     2221 actgaaatat ttccctgatg ggcctccacg atggcttttg tataactcaa gcccaatcca
     2281 aatcctttca cattgtgaat atttcctgta ggaacacggt ataatttgtc gaaaatcatt
     2341 ccttgatttt ctttactaat tccgatgcca ttatcaatca cttcgaatac aaaattctga
     2401 tcgcggttat aagtatttat cctgatggta ggccttatgg gagaatattt aatggcatta
     2461 tcgagtaagt ttactacgac gcttgtaagg tgcaggcggt cgccccgcac catgtcgtgt
     2521 agggcttgga gggatgtttg tatgctgcct ccggcggttt ctgcctgtat accaaccttt
     2581 tggacacatt ctgtaattat ttcatgcatc gcacaaggta ccatgttcaa ttcgagtttt
     2641 ccctgctcga gtaccgatgc tatgagaatt ttttcagcca ttgtacctaa acgtctgttt
     2701 tcgtcggtaa ttatctgaat atagttcgaa gccatagcgg gtgtttttac cacatcggga
     2761 tcgttcaagg cttcacacgc aagcgaaatc gttgaaaccg gagtcttgaa ttcgtgtgtg
     2821 atattgctaa taaaatcgtt cttcatatcc gataatttct tttgatacag aatagttgaa
     2881 atggttttat agaatatggc aataaatgta ccaaccaaaa gaatggaaag taaaagcaac
     2941 caccatatct gttgaaccat atactgtcgt tcgtagggaa aataaaccag caggatggct
     3001 ttatctttgc caggttgacg gggaaacaga ttaacagcaa aggctttatt cagaagctcc
     3061 tgagtaaact tgccagtttt ttgataaatg agactatcgc gtctcgtatc gtatatacca
     3121 aattcaaatt ccgtcttgat gcctttattc tgaagctcta ctgccagcat agaatctact
     3181 acaccgggtt gtattctttt ttcgagtggc atttcgatga tatcgtcgaa acgaccaaga
     3241 gctgcatcat agataaggga acggctcatc agctcctcca ctacgctttc tctttgtttt
     3301 tgcagtcttt tgtaaacagg atcatcttcg atatttacag atcgagtttg tatagtttga
     3361 atttgttttg gctcgattaa agaatcgagt tcttcctgaa tattcctatt ttcacgaaat
     3421 tctgaagttg tgtctaaatc acgcagagta cgccatgatt tacgatcctg aattattttt
     3481 cgacgtgctt ttacatcggc tgccgaactg ccatcttccc cagttagctc atggtaacgc
     3541 ttatcgatta aataattcaa cgaatcgatc tttcggtaaa gtcgggcact ttccttgaag
     3601 ctgcgcaaac gcttgccaga ttcgatcctc tctaaagaaa gcacaacatt cgatacggct
     3661 tcttcaaccc ctgccgtaaa tgcagcttca cgcagcatga gagcattcct gatccagtaa
     3721 acctgaataa acaataggac caccaataca aagcccatta aaaagatgat aatagcgaga
     3781 atacgtctgt tcattttcgt tacaaaatta actgaaaatc aaatgaccat cgaaattcta
     3841 tgcctttttc gcaatcttct aaaaaaatcg aaaaatttta aaaaatttag tctgtttgtt
     3901 ataaaagatg ataaaaagag tgagatatcc tgtaattttg taagattaaa atcctgcaat
     3961 caaataatcc aaaatcccat gaaaagagta gtgtgttttt taggaatggc cttgtgtggt
     4021 gtcattttat taggtggact gcaatcgtgc gataaagtgg aaaatcttgc caaattcgat
     4081 gttaagatgg atttaccatc ccttaatttt aatttagtgg catctgctaa aaactcctca
     4141 actcaggaat actacaaaga tttcaatgtt tatttaaatc tcgatagtat tcaggaagct
     4201 cattcattga attcgttttc catcgaaaat gggaaactta cgaaagcaac cttgcgcatc
     4261 atttatccag caggagcaaa ttttcagttt ttgatcaact ctcgtttggt attgttcaac
     4321 agcagcaatc aggagatgca agtggctcat actggcaata tcaatgccaa tgccactgaa
     4381 attgaattga ttctggatac tgccgatctt acttcgttat tcaaaatgaa gcattatact
     4441 ggcagaatct attacaccat agagccctcg ctgttgcctg ctcaaaatgt taccatacag
     4501 ttgaatcaaa gtatccagtt tactgtacac cccctgtaga atcccacatc tttgtccgaa
     4561 ggcattgaaa atcccggtgt gaatagcgct gggatttttt atttttgcat catgataaat
     4621 gttattctaa ctcatcaata tattttttcg atatgttacc aacgatagat cctacaacaa
     4681 ccaaaagctg gaaaaaatta agtaattatt tcacgagcat cggcaagcaa agtctgaatc
     4741 aattattcga aagtgataac aacagagcac aaaaattttc tcttcaatgg cagaacttgt
     4801 tcgttgatta ttcgaaaacg catatcgacg aacaaactat gcgattgctt atagaacttg
     4861 ccagggagtg ccatctcgcc gaagccatag agcaaatgtt tagaggggaa aaaatcaatc
     4921 aaacgcaaaa aagagctgtt ttacattttg ctttacgcaa ccttggcgat gatccagttt
     4981 ttcttgacgg cagggatgta atgccagaaa tacgaaccgt acgtaatcag atgaaaattt
     5041 ttagcactca ggtaagaaat ggtgattgga aaggatatac cggtaagccc atacggaata
     5101 tcgtcaatat tggaattggt ggtagcgatc ttggcccggc tatggtttgc agagggttgc
     5161 agttttatgg tcacgaaaga ttaagggtat ttttcgtatc gaacgttgac ggaggtcata
     5221 tttatgaaac tcttcgccat ttgaatgccg aagagacttt atttcttatc gtttcgaagt
     5281 cgtttacgac tcaggagaca ctggctaatg caaaaactgc ccgacaatgg ttcattaatc
     5341 agggcggtaa agcggaagac atcagcaaac attttgtggc tatctcaaca aataccccgg
     5401 cggcaatgga atttgggatc gatcctgaaa acatatttca tttctgggat tatgtaggcg
     5461 gtcgattttc attgtggtca gccgtagggt tgagcattgc attgcaaatt ggttatgaaa
     5521 gttacgaaga actattaaaa ggtgctgaag ctatggatca tcacttcaga gaaactccct
     5581 tcgaaaacaa tctcccaata ttgctggctc ttttgagcat ttggtacacc aacttcttcg
     5641 attgtgccac agaagccatt ttgccttacg accagtatct cgaaagattt cctgcttatt
     5701 tgcaacaaac cattatggaa agtaatggca agtcgatcga ccgcagcgga ttgcctgttt
     5761 cctacaaaac aagtccggta atttggggtg agcctggtac aaatggtcaa cattcgttct
     5821 atcaactcat tcatcaggga actcaaatga ttccttgtat ttttatggct gccgccaaac
     5881 ctttaactcc attaagtgaa catcatcagc ttttgttatc aaatttcttt gctcagcccg
     5941 aagctttgat gaagggcaag acagccgaag aagtgagagc tgaaatgttg tcgaaaggtt
     6001 ttaatgaaga agaaattaat aaattacttc cattccgcgt ttttagagga aatattcctt
     6061 cgataagctt gctttatcag caacttacgc cttataatct cggagccatt acagctcttt
     6121 tcgagcatag aactttcgta cagggcatca tttggaatat cttcagtttc gaccagtggg
     6181 gtgttgaact cgggaaaagc cttgccaaca atatcattcc cgaactggcc cacgatgatc
     6241 ccattcaaac acatgatggt tctactcgcc aattgattca caaggtgaaa caaatgagaa
     6301 aatcctaatc cttttctaaa ggctttaatt cctaaatttg cagaatcttt aaacattatt
     6361 tctttgtcgc aggaagaaac acttacacgc aaacgcttac gctcagcata tattactacc
     6421 atcgtgagtc taacactggt acttttcatg cttgggcttt ggggagtgat tgggcttcat
     6481 gcacacaaaa tatcgaggta tgtaaaagag aatcttggtt taaccattta tttccagaag
     6541 aatgcacgtg aaactgaaat tctgcgttta aaaaaaagct tggataccac gaggtgggtt
     6601 acctcgacac attatatatc gcctgaagaa gccgagcaaa tactaaaaaa ggaacttgga
     6661 gaggatttta ttggtttcct cggctataac cccctcttgc cttgcctgca aataagatta
     6721 aatgcggatt atacccatcc cgattccatc caatatatcg aaaagcacct taagcaaaat
     6781 cctaaagtgg cccaaatcga atatcaaaga gacctcatca acagtgtaaa taaaaatctg
     6841 aatatcatca gtatcattat tgccgttcta tcgtttttga tgtttctcat ttcaattggt
     6901 ttgatcaata ataccataaa actttcagtc tattctaaac gctttatcat tagaagtatg
     6961 atgctggtgg gagccactcg cggtttcata agagctccat ttgttaaaaa gggtttgtgg
     7021 catggtatac tcagtgcgat ttttgctatc atcttgcttt caattttgct ctatgctgct
     7081 tatttgaaaa ttcccgaact taaaacattg gccgactttc atattctaat gataattgct
     7141 ggagctatga tagttaccag cgcattgatc tcggtaattt cgaattatat cgccgttacg
     7201 cgtttcctca atatgaatat tgacaagctg tatatgtaat aacttttctc ttatggaaaa
     7261 aaaaacgata gttacctcta aaaaagcaaa agaagaagaa aaacctcaag ccggatttgc
     7321 ttttgaacgt cagaactata ttctgatggt tattggcata gtcgttctca tcattggctt
     7381 tttattgatg ataggcggag gaagtgatga ccctcaagta tttaattacg atttattcaa
     7441 tttcaggcga cttacacttt cacccatctt gattattgct ggattcatca tcgaaatctt
     7501 tgccatcatg tggaaccctg ggaaaaaagt taaaaaatga cctggctaca agctctaatt
     7561 atcgctattg tagaaggcat taccgaattt ttacccatca gttccactgg ccacatgatt
     7621 atcaccgagg ccgtcttggg cattgaaccc agtgagtttt cgaagacttt tatcgtcaac
     7681 atacaatttg gagccatact gtcggtaatt gttctgtatt ggaagaattt tttcgcttca
     7741 cttgattttt actacaagtt atttgtggct tttttacctg ctgcggtagt tggatatctg
     7801 gctggcgacc tcatcgaccg catgctggga agtgtattgg tagtggctat ctcattggtt
     7861 gttggaggaa tcgtgctttt attcattgaa gaatggttca aaaatactgc cgagaaggga
     7921 aaaaatgatg ttacctattc ttcggccctg aaaatcggat tcttccagtg tatagccatg
     7981 attcctggaa tatcgcgttc agcagctact atcattggag gaatgaccca gaaactgaac
     8041 cgaaaagccg cagccgaatt ttcattcttt ctggctgttc ctactatgtt tgcagcttcg
     8101 gcttataaac tcatcgaaaa ttacaagtca atcaacattc acaatattcc tatacttgtt
     8161 tttgggaacg taatagcctt cattattgct cttctggcca tacgaacctt tatcaactac
     8221 ctcacgcgtc atggtttcaa actctttgga tggtaccgca tctttttagg cggtcttatt
     8281 cttgtcctta tggccttggg catcaatctc aatatcatcg gatgattcga atggaagaag
     8341 acaacatact ccttttcgat aaaccttacg gatggacttc gttcgatgtt gtaaacagtg
     8401 tgcgttacac cctcaaaaaa tttacgggaa agaaacatct gagagttgga catgctggca
     8461 ctctcgaccc tttggccact gggctgctga taatatgctc tggaaaagaa accaagaata
     8521 ttgaaaaata tcaagcactc gaaaaagaat atacaggcac ctttcatctt ggagctacta
     8581 ctcctacttt cgatctcgaa tcggaacccg gaaatttcaa agatatctcc tttcttaaac
     8641 ctttacatat acacgaagcg gctttacggt ttaccggtga gcaacaacaa ataccgcctt
     8701 tattttcagc cattaaaatc gaaggaaaac gagcctatca atacgctcga gaacaggaag
     8761 aaatcaacct tgaccacaaa accatcatta tttatcaatt cgagattatt cgtacagaat
     8821 ttcccgaagt cgacttcagg atcgtttgta gtaaaggaac ctatatacgt gctatagcac
     8881 gcgattttgg cgaatatttg ggatgtggag cctatctgaa aagtctgcgc cgtacccgaa
     8941 tcggaaattt cgatgtaaaa gatgccttcg atatcgaaac cttcaaatca tcgctctacc
     9001 ataagttaga ataagtctat cgatgattga gtcttgcact tcggcctaaa ctactggaat
     9061 tttttaaatt tgccattcgt ttttgtttta tgaacgctcc acctaaagac aatacaacca
     9121 gcagttcgag aaagaaaatt gcacggcagg caatttcgaa gctcttagtg ggaattctca
     9181 tcgttatcct tgtcaacatc attggttctt attcatttac gcgcttcgat ctaacttcgg
     9241 aaaaacgtta cacactttcc gaatctacta aaaagcttat aaaagaagta gatgattacg
     9301 tttattttag aatttatctg gaaggcgatt ttccagctgg attcaagcga ttgagaaacg
     9361 aaactcgtga gatgctcgat gaaatgaggg cttataacaa attcattaat tatgagttta
     9421 ttaatcctaa tgcaagtagt aatcccaaag accggcaaaa catatacagg caattgatcg
     9481 agaaaggact taatcctacc gatttacaag ttaaagataa ggaaggcgaa agtcggcaga
     9541 tcattttccc aggtgccatt gcctcatatc gggggaaaga aactcccgtt cagctcctta
     9601 cgggtcaaat gggggcaagg cctgaagaaa tcttgaatct ctcggtcgaa aacctggaat
     9661 ataatttagc caataccata cgaaaactca ttgtatccta taaaccgaaa gttgccttta
     9721 tctatggtca tggcgaactc gaagaggctt ttatctacga tatttctcaa agcatggccg
     9781 aatattacac cgtcgaaaga atcaaactcg atggccagat caatgcgctt accgaacaca
     9841 aaaccgattc aggaggtatt tccattttca ataaatataa ggccatcatc atagccaagc
     9901 ccgactctgc attcaccgaa aaagacaagt ttatcatcga tcaatttttg atgcgaggtg
     9961 gaaaaattct gtggtttata gatggagtaa atgcagaaat ggacagcctc cagaacaaaa
    10021 gcagtacttt gggcttggca cacgacataa atctcgagga tatgcttttt cgctatggag
    10081 tacgtatcaa taatgatctt gttttagatg ccaactgcct tccgataatg atggttacag
    10141 gaactattgg caatcagcct cgctttagct ttgtaccatg gattttttcc ccattggcta
    10201 atccagctac aaaacatccc atcgttaaca acctaaatcc tattaaaaca cagtttatta
    10261 gcagtataga tacaatagcc atcaagggag ttaaaaaaac catcatgctt tcgagcgggc
    10321 aatattcgcg taaaattatt gcgccgatta tcattgatct caatatagcc cgtcaagaac
    10381 ccgacctgaa gttgttcaat caaccttata ttcctttggc cgtgtttctc gatggaaaat
    10441 ttccttcatt atatgaacat cgtatcccag ctaatattag cgatagcccc gaaataggat
    10501 tccttgccga aagcaaacca actcaaatgc ttgtggtgag cgatggagac gttatccgta
    10561 accagctcga acgtaaaaat gcatatccct tacctttggg ctacgaccaa gatacgcgac
    10621 aaacttttgg aaataaggag tttattctga ataccttgaa ttatatgctc gacgactcga
    10681 aattggttac cattcgttcg agagaaataa aaatgcgcct tctcgatcct ctaaaactgc
    10741 aaaacgaacg tttgcgctgg caaaccctca atattgtgtc ccctgtagcc ttagtcctgc
    10801 tttttggcct catcaagtat tccttgcgga aacgtcgcta cgcgcattga tatctaattc
    10861 aatatttttt tattctccct cgaaaagaaa gaaaaattat cgatataacc tgaatttccc
    10921 aaggaaaaca tcagggttct tgccatgaat cgatcgataa ttctttcaga atttatttta
    10981 tcttttcaaa aatttcagag cagcatggtt atcttcagtt tctattctta atatataaat
    11041 gccccgcgcg atgcccaaca agcttacgtt cgattttcca aaccccaaat tgccattctt
    11101 tatttctatg ccatcgacag aaaagagcga ccacttcccc tgattttgtg aaatcaactc
    11161 gattctatcc gcatagaccc tatagtcgaa aaactgtttc ttctcttgga ttggagcaat
    11221 gcctgtatag gcaattgaaa aagtttcttc tggggcaaat tctgaagccg aataggaagg
    11281 gtcgatagtc tgtacgctcc agaaataatc cgcttcgcgt agcccttgca aaatccagaa
    11341 tgtatcctga gttacattgc tgaaacttgg caaaagcatt tgcccgctca gtacattgct
    11401 cgccgtcgac atgatttcgg agctacccgg attaagccct acccttacat tataaaatat
    11461 attcttacca ttagtattat atggagatgg tttccatttc aatatcaact catttccatt
    11521 catctccgat gacaagtcaa taggagcgaa ggggggcaaa tttggaacga atccattcga
    11581 attcagggta ttagagtaaa gaacggtttc aggattcccg ctggtagaaa ttcccatcaa
    11641 caggaggtcg ctatcgccgt cattatcgaa atcggcccaa ctagaatagc tatatgacaa
    11701 atctacaagg ttttgagagg aaggccaaaa agaaccaatt tgattattgt agaacaagac
    11761 gctcatcatg gctccacaac cgggcgcctt gcccgttgtg gcaaaatcag gatatccatc
    11821 gctattgtaa tcggcaattg aaatgttacc caatgccaaa cccgtaattc caacaaacac
    11881 ctcctgtaaa ctaccatttc catcatttct gtatattttg aaaattggag tcagcgtcaa
    11941 gtcattgccc atcagcaaaa tatcatggtc tccgtccttg tcgaaatcca tgaacagtga
    12001 gaaccctgcg cctacacctt ctatattggt tgggatctca ataaatagac ccatatcgtt
    12061 gcggaatagt ttggtataat aagctcctcc aatatctcca cttagcaaaa gatcgaaatc
    12121 gccatcattg tcataatctc cataactaag cgatgaactg atgactccag gaaaaccaaa
    12181 atctacttct tcgaagttat catttcccaa gttatgataa attcgtgtaa atcctttgta
    12241 agaaagcgta tctccactta agaatatatc aagaaggccg tcattgtcat aatccacaaa
    12301 gatggccatt ccattataaa tgccttggaa tgtattggat gattgcgaaa agctaccatt
    12361 atcgttttta taaagcaaac tatgaggctt tgagctgaaa tcgcttccac aaaccaacag
    12421 gtcgtagtcg ccatctctat cataatcacc aaatcgtagc gagccatttt tcaatccaat
    12481 taggttggca tcgatatcaa taaacacacc ggcattgttt ctaaagattt ttgttaaagg
    12541 ttggccaata gaatttgcgc ccatcattgc caagtcgaga tagccatcat tgtctatatc
    12601 tgcccagtca caactgccct gagcaacgtt agggagggta gtcgaatagg gaacgaaatt
    12661 ctgtgaattc actaaatacg gaactgaaat taatattgca acgactaaaa gatctatcag
    12721 gttttttgtt cttttcataa aaattctcga tgaaatttaa taaaatttta aatctataag
    12781 tccaaccgaa gaggaaacac ccgatagggg caatttattg ctatcgcgca tgcaatttat
    12841 tattttgttt cgtatgttaa atcgatttac agacagatag ttcggtaaac agggcagcag
    12901 ttttctgttt tcatggtaat cgttgaacat gcggaagttg acataagtgg taatgcccat
    12961 tattttttca ttcgaatcga tcgtatctga aaagtccatg ataggtctcc attctttgtt
    13021 ttttataata gatttttcat gaaccgtctt ctaaaaaagt atttaaagaa ttttcgttta
    13081 tactttctcg ataatcgaag caattccctg ccccacacca atgcacatag tgcacaaagc
    13141 tctatgaaga ttgttgtttg acatttgatg tacggctgta gtaatcaggc gggcgccact
    13201 cattcctaaa ggatgcccta aggatatggc tcctccctgc ggattaatcc ggggatcatc
    13261 ttcctttagc cctaacaatc gcatgcaggc aagcgattgt gaggcaaacg cttcgttcag
    13321 ctcaataagg tcgaagtcct cgagagaaaa atgaagtttg aaaaggagtt tttgcgtagc
    13381 tggtacagga ccaattccca taatacgagg aggaaccccg gcggcttgca cgccgacgat
    13441 tctggccatt ggacgcagct tatatttttt cagagcattt tcagaagcaa tcaataaggc
    13501 ggcggctcca tcattcactc cagatgaatt ggctgccgta actgtcccac caggcctcga
    13561 tacaggtctt aaagttccta ttttttctaa ggaagataaa cgtggatgtt cgtccattga
    13621 aaaaacaaaa ggttcagatt taggccgaac aatttcaact ggcgtaattt ccctttccat
    13681 agctccactt tgaatggcct gatgtgcctt aagctgacta tgataagcaa aaatatcctg
    13741 atcttcccgg ctaatatgat acattcccgc tacattttcg gcggtttcaa tcatggaatc
    13801 caccccatag agttgttcca tttttttatt gataaatctc cagcctatag tagtatcata
    13861 aatttgaaca tctctcgaaa aggcttccga tgccttaccc atgacaaatg gagctcttga
    13921 catgctttct actccaccag ctattatcaa atcggcatct tccgttcgta tcatgcgcat
    13981 ggcctgtcct atggcctcca ttcccgaacc gcacaatcta ttgactgtta cgccgggaat
    14041 tctttcgctc aagcctgcca gaagcaaggc cattcgcgcc acattacgat tgtcttcacc
    14101 agcctggttg gcacatccaa aaattacatc atctacctct tcccagttca ccgaagcatt
    14161 tctctccatc agggccttga tgggaatcac ggccaaatca tcggttctaa tggaggccaa
    14221 tgttcctcca tagcgtccta taggcgtgcg aatagcatca cagatgaaag cttctttcat
    14281 gcttcgtcgt ttaatcaaac attcaacagg ggcttgatga acttaaagcc ttcaagtgca
    14341 gtttccgaaa gcttatcgat atgttcctgt ttcattaccg tgctaaagaa acatgaattg
    14401 gaatagacaa gaataattcc atgatcatac ataaaatcga gaaatgtatt cacggttttt
    14461 ttatggttgg catcttcata acactcgcga taatttgagg gtagtgtttc tctaaaatgc
    14521 attttcatca tagagcctgc accggcgacg gataatggaa ttccggcaat tttagccgct
    14581 tgcatcaatt gttctttggc ttcctgagcc agatgattta ccttatctac agcttgtttg
    14641 tcgaacattt ccactgctac gcggccagcg gtcatggtga tgggattagc cgagaaggtg
    14701 ccagaaagag aaaaccgcaa gggggtttgt aaaggattaa gaacatccat aatttcagct
    14761 ttaccagtta atgctccaac ggggaaacca ccccctatca attttcccaa agccgttaag
    14821 tcgggtttta ctctataaag tgcctgagct ccttcgtaat ttattctaaa acacaccact
    14881 tcatcgaaag ccatcaatgt attgtgtttt cttgtccaat catacactgc ttccacaaat
    14941 tcatctgtag cccgaatcat tcctatacgg tgaggcacgg gatcgattaa tacacaggca
    15001 atttcatcgg catgccggtc gaggatggcc aaggttcttt ccacatcatt atagggaaag
    15061 acaattacct cgtcgagcac actgggcgga gtattcctta cgtgaggtac actgttgggt
    15121 tgctcaacag gtccccaatt atcaggagaa gccgattcac taacttcagc gaaatcgtag
    15181 cttccatggt aagctccttc ggctttagca atcttatgtt ttcctgtgta agctcttgat
    15241 gcttttatca acgacatcac agcctcggtt ccgctattca tgaacctgat tttttcgaat
    15301 cctggaactc gatgacaaag tgtttcggca tgacgaatct ctgcttcggt tcccatcgaa
    15361 tatgcagttc ccttctgaag ctgctcataa acagctttta caatggcagg atgtgcatga
    15421 ccatgaacga gagaggcaat attgttgaca aaatcgatac gtgttattcc attgacatcc
    15481 tcgacatagc agccatgagc cgaagccaca tagtgcggat gtggctgacg aaagattaca
    15541 tttcggctga tacctcctgg
//
LOCUS       DAOORU010000040        15543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4634_length_15543_cov_5.846720, whole genome shotgun sequence.
ACCESSION   DAOORU010000040 DAOORU010000000
VERSION     DAOORU010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15543
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4634_length_15543_cov_5.846720"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /gene="menA"
                     /locus_tag="PLF20_04100"
     CDS             complement(<1..808)
                     /gene="menA"
                     /locus_tag="PLF20_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HOX80225.1"
                     /translation="MKETILKEWIDAARPRTLPLSLSSTLMGSFMALSFHQFSWSVFI
                     LAMLTTVMLQILANLANDYGDTLSGVDNKERIGPKRGLQRGAISLQAMRNAIIINVLL
                     SVIFGIWLILEGLHYRISIMALIYLLMGFAAILAAIKYTVGRNPYGYVGLGDLFVFIF
                     FGPVGVAGTYYLHTNTWDPCVWLPSISLGLFSVAVLNINNIIIHSFVNISIIIRTLMS
                     RYMSFNNSLSTYSFNLWDTLSIIMSLKADYFTAFSFCGTFCPARCPAKPLF"
     gene            complement(805..1125)
                     /locus_tag="PLF20_04105"
                     /pseudo
     CDS             complement(805..1125)
                     /locus_tag="PLF20_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
     gene            1612..1983
                     /locus_tag="PLF20_04110"
     CDS             1612..1983
                     /locus_tag="PLF20_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOX80226.1"
                     /translation="MKNDHLILRMNYKIWMETEDGTPILGEGKCKLLKEIQRTGSLKI
                     AIENLGLSYRKTWNNLNRIEKKIGFHLVEKQRGGKEGGHTALTPQGEKVVEAFDRYYQ
                     EVDPLVKDLVGKVVLKFPHAG"
     gene            2073..4463
                     /locus_tag="PLF20_04115"
     CDS             2073..4463
                     /locus_tag="PLF20_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX80227.1"
                     /translation="MKQTKITQEKPLSFDRFKKMVLKAIGRLGNPLPSIMLVCMAILL
                     GPAQVYSQNIVKGKVLDANSHAAIRGANVIEESSKVGTTTSTSGEFSLEISKDVQKPV
                     LIISYLGYKTQKFAVYVEGKKFIEVFLEPEVYMVPELIVLGQRLSSKQNENAARIYVV
                     PEEEIHAMPKLSVDEILAELPGVVVSRGSGILSSSATVSMRGMGSEQGRTLVLLDGIP
                     LNKADGGSVQWNNIDLNSIKQVEVLKGPGSSYFGGNAMGGIISMVTAMPQKKIEGQAA
                     LEYGTYNSMGAHLNVGGKTGKFSWNAAGRGRKSDGYIVQPPDEIDSTVIKTNMEEIGV
                     NLKARYDIDTKQYVQVSYSYFDDKRGGGNNFHPFNEMAEPDGTYTSHTNNYINVNYGF
                     RADKILADVRAYYNDEDYVTISESLKGTQFTKYDVDSKRTDMGLMSSATLPFQNGHSL
                     TFGLDLKSGKVDGNDIYKTSTDKVINQGTNFNSGLFLMGNIQLLKGKLMLTPGLRWDY
                     AHFYDGVFVVENPTKVTAFLVGFQDTMPTADWNAFSPRLGLQYNFNPDTRIYAQASSG
                     FRPPILDDLCRTGRMRLGLKLANPNLKPERIYNFELGADYRYKLITLSLSGYYSRGTD
                     FIYYLATGDSAVVSNKLRPVLIKDNISDVDIYGLEFITKLQLPAGFNLKANCAYTSTE
                     IGNYIVKFPGREEDLTGKELVMVPKFQVGAGLYYSYKFVNAAIVYRFTGKQWIDDSNK
                     EELQETNLFDANLTLHLHRYIDASVAVYNIFDRLYYDTKGLMTPGRFIMGRLTFKL"
     gene            4502..5521
                     /locus_tag="PLF20_04120"
     CDS             4502..5521
                     /locus_tag="PLF20_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80228.1"
                     /translation="MNKTIYSSFILLFFICMQNFCVFSQTNDFYDDQPTIPLGDVQIK
                     VEGEIEQHYHVDFTKLPLRSVIVKEAVLDSEGDHFIGAYRYDGYSLYDILNEAVLKKA
                     NQAEFPPVIDLFVEVEGVNGDKAIFSWGEIYYPIHRHEIIIATQISRIVPSKTKDYWP
                     LPGSPRVVAAADLHSHRYIENPKSIKVRSWNRPFVKHEEAKFTNKISLEIDGEKKMEI
                     VPSSLKPPISKYSTVFYGRGRGIHGISTFEGKPLKNLLSTFFKINPQILMEGLLIVYG
                     IDGYRSVFSCAEVMDRNDQEEILLVFNDNLDNPEAAGFRIFPACDFFSDRAVKAIEKI
                     EVVFK"
     gene            5804..6856
                     /gene="thiL"
                     /locus_tag="PLF20_04125"
     CDS             5804..6856
                     /gene="thiL"
                     /locus_tag="PLF20_04125"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HOX80229.1"
                     /translation="MAIENEKSKRTELSELGEFDLIDRLTEGLTPLNHSTIKGVGDDA
                     AVMQYDNHSLILLANDILIEGVHFDLTYTPLKHLGYKAGVVNFSDIYAMNGAPKQLVI
                     GLGVSNRFSVEALDEFYEGLRLACHHYGVDLVGGDISSSKTGMFISISVVGEVSKDEV
                     VYRNGAQLDNLICVSGDLGAAYMGLLLLEREKRVYLANPNMQPDLEGNDYVLERQLKP
                     EARKDIIRLLHEACIQPTAMIDISDGLASEILHICKQSNKGCVIYEDKIPIDPATLLL
                     CDEFKIIPAIAALHGGEDYELLFTIPLSEYEKIEKVENISLIGHITDSSEGCQLITND
                     GKQIPLQAQGWNHLRK"
     gene            6876..8693
                     /gene="uvrC"
                     /locus_tag="PLF20_04130"
     CDS             6876..8693
                     /gene="uvrC"
                     /locus_tag="PLF20_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOX80230.1"
                     /translation="MTKSDEQINEHLSQILQTLPHSPGVYQFFDENQKIIYIGKANDL
                     KKRVSSYFTKETGVSGKVLVMVRRIRSIEVIVVDTELDALLLENNLIKEHQPRYNINL
                     KDDKTYPWLCIKNEPFPRIFPTRNPVKDGSKYYGPYASGRIMNNLLELIHQIYPLRNC
                     NLKLTFRNIEKGKFKPCLEYHIGNCKAPCIGLQTEEEYDANIASIKDIIRGNLSSVAS
                     SLKREMLAHVENLEFEKAQRVKEKLELLEKYQSKSAVISNTDLHADVFSIVSDEKIAC
                     VNFMKVANGAVIQSHTIEIRKKLDESDEEILSFAITELRLRFESQVREIIVPFKLTID
                     FPGILITVPKKGDKRQLLELSLSNAEYYRLEKLKRIELVDPERHSKRILHQIMKDLRM
                     NQLPEHIECFDNSNLQGTEAVAAVAVFRNAKPSKSEYRHFNIKTVEGQNDFASIEEIV
                     YRRYKRLLEEGEGLPQLIVIDGGKGQLASALFSLEKLGLKGKITIIGIAKRLEEIYYP
                     GDSLPMYLDKKSETLRLLQHIRDEAHRFGITHHRKRREKAFIQTELTSIRGIGEATAK
                     NLLKAFGSLENIKKAEAAEIEKVIGKAKTKLLLQHFNQS"
     gene            complement(8680..10497)
                     /locus_tag="PLF20_04135"
     CDS             complement(8680..10497)
                     /locus_tag="PLF20_04135"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HOX80231.1"
                     /translation="MSNSWIDRVRERINIFLYPHKETLLWVLRILSIIVSLVAVCAIV
                     YYHGFPRTPHTDDIVRIITIFSLAFYALKYFLNFFYDFHPLAYLRRTWFEGILVLFII
                     LMGLFYLIVGQQRIYGFFAQRGMEQFPDLSILFIQFYVFIMVLLETAKAGQYIDRIKI
                     GPAGLLALSFLILIAFGTAMLLLPEMTVDHHIQPVDALFISTSASCVTGLSTVDISNY
                     FTFKGQVVILILIQLGGINIISFATFFALFYTGAEGLKQQSLLKELLDTSGLSDARRI
                     LKRIILYSFTIEIAGSIILCQLWEKDLSIASPGGVGFAALFHAVSSFNNAGFSIFPNG
                     LMFEPVRMNLASHWIIAILIVLGGIGFSVLQEIFNFKLLLTKLRTAWKHFQVQTKIVL
                     ATTVCLIILGALGVFLLEYNHSLYGLKLSDKITTVFFQSITARTAGYNTVNIGALSQA
                     TLFLLIILMFIGASPGGTGGGIKTTTFLTLIKASIATIRGKKRLEAFKHTISFETVDK
                     AYSVALLSLILIVTGTFVLTVLQPDFSFTSILFEEVSAFGTVGLTMGITPFLTSASKM
                     VIIITMYAGRVGTLTLAIAFLKKASFKDYAYPKANIMIG"
     gene            complement(10525..11220)
                     /locus_tag="PLF20_04140"
     CDS             complement(10525..11220)
                     /locus_tag="PLF20_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOX80232.1"
                     /translation="MVHRKIAVIGIGRFGESIAKNLSKKGAEVLAIDKLQENIERVSD
                     DVSLAVAIDATDKKALLSQNIQEFDAVVVSIGESFEQRLLCVALLLDLGVKRIIARFG
                     GKSQRIILEKLGVKDLWSPEEEVGAIVAERLLNPSVVSYLQLPDDYRIVEMLTPKKVA
                     GRMLGDLDLRDRYKLSLITIKEETTTKVNNMEMSIYHVAGVPDSKYVIKLTDRLVLFG
                     KNKDIDRFIQINE"
     gene            11728..13491
                     /locus_tag="PLF20_04145"
     CDS             11728..13491
                     /locus_tag="PLF20_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOX80233.1"
                     /translation="MKPFIGIVFCVFSILPSRIFAKDQNWTTYCESTDFRRTPRYKET
                     IHFCKQLAENSHIIAYEEFGISPQGRALPLLIMDKDRNFSPELARRAGKAILMIQACI
                     HPGEPEGKDAGLILFRNLGILGMDTSLLRHVTLVFIPIFNVDGHERFSPYHRINQNGP
                     EEMGWRTTAQNLNLNRDFMKADAPEMQAWLRLFNYWNPDFFVDTHTTDGADYQYVLTY
                     SLETGPNTDSSLGKYLRENYIPTMEAYMSEKGYKVFPYVNFKQWHDPRSALYSLPASA
                     MLSHGYVAARNRPALLIETHMLKPYKLRVSSTLELLFFTLKHLNDHYRDYLQINRQAD
                     TYTSSADFRPFPLKFNFITSNTDSVMVDFEGYDYRIEKSDLTGGNWFIYLPRPKTFRL
                     PNFNHAIAQDSILLPDYYIIPPEWTTIIERLRLHGIEMNFLTYDTILKVESYRFSNPQ
                     WANFPFEGRNRLSVKTEKYVERRAYPAGSALISLQQPTARIIALLLEPQSSDSFLTWG
                     FFNAIFEQKEYAETYVMEKIARQMIEKRPDLLPQFQAWKEKNPQMDKDQWMQLNWFYQ
                     HSPYFDQNLNKYPVGRIFLYQ"
     gene            complement(14554..15342)
                     /locus_tag="PLF20_04150"
     CDS             complement(14554..15342)
                     /locus_tag="PLF20_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOX80234.1"
                     /translation="MMNIAVMQLDLKWEEPLMNRHKVENLLTSLPNETDLILLPEFFT
                     TGFSVKNIGLAEPMNGPTVSWMQKIAKEMNCILAGTIPIIDHGKYYNRALYVSREGVM
                     ASYNKRHLFSLGSEHQLYTPGNKNVIVEYKNAKIQPQICYDLRFPVWSRNRYSNFQFA
                     YDILIYHANWPDPRGCVWEQLLIARAIENQAYLVGINRCGQDGEGLSYSAPSMIINPK
                     GNIIAQMEPAKEGVLSANIDLGELKRFREKFFIAPDWDNFDVSP"
BASE COUNT         4977 a         3129 c         2990 g         4447 t
ORIGIN      
        1 aaaatagtgg ctttgcggga catcttgcag gacaaaatgt cccgcaaaaa gaaaacgctg
       61 taaaataatc agctttaaga gacattataa tggacaaagt gtcccataaa ttgaagctgt
      121 aagtggaaag tgaattgtta aaggacatgt atctggacat caatgtccgg ataataatgg
      181 agatattgac aaaagaatga atgattatat tattgatatt caatacagca acactaaaca
      241 atcccaatga gatggaaggc agccaaacac aaggatccca tgtattggtg tgtaagtaat
      301 acgtaccagc aaccccaacg ggtccaaaaa atatgaaaac gaaaagatca cccaatccaa
      361 cataaccata agggttgcga ccaaccgtat atttgatggc tgctaaaatg gctgcaaagc
      421 ccattaataa ataaattaaa gccataatgc ttatgcgata atgcagacct tctaaaatga
      481 gccagattcc aaaaataacc gacagaagaa cattaataat aatagcattt cgcattgcct
      541 gaagagagat ggcacctctt tggaggcctc gttttggtcc tatgcgttct ttgttatcta
      601 ctccgctcaa ggtgtctcca taatcattgg ccagattggc cagaatttga agcataacgg
      661 tggtgagcat ggcaagaatg aatacgctcc acgaaaattg atggaacgac agcgccataa
      721 agctgcccat caaggtactg gaaagagaca gaggcaatgt cctgggtcgg gcggcatcta
      781 tccattcctt taatattgtt tctttcataa aaatgatgac ttaagaaatt taggaaattt
      841 cgaaaaatcg agtccgcatt tttcaagaaa agctttttta tcttcctgtg attcttcgat
      901 gcaataataa agcaaggtag ccttatcggt cagtttccgt atgccagcct gtccatcaag
      961 ttcgacattc aacgacattt tgatcatcct tagtgcctgt tgactgtgtt tcatcatggt
     1021 tttaaaccaa ttcactactt tatctttaag cttattgaaa ggtactacct tatttataag
     1081 cccaaattcc aaagcttttt gaacagtata ttgacggcaa agatagtcac ttaacctcag
     1141 gaacgacggg gaatggtaac tctttcttcc ttgttcttga aaatttagtt ttttcttttg
     1201 ccccttaaac taaccatccc aaaagtttgc atttgcaata aaaatctaag tacaaataat
     1261 tcattgaaac tgtaatatcc gaaaaatcaa ccatcgagag tcattgtggg ttcctctgcg
     1321 cggcagtgta gttcgtttat ccgcttcatt ttcatattat cgtggtttgg ataagcatac
     1381 agattcgaaa tcatttttct ctcccttatc aatttattgg caaacatttc agtaattcga
     1441 gcatatagtt cggttcagac agtcaaataa aaggtcttct gagcacttgt acaagataaa
     1501 aggcgataat tattttcgac gaaattttat ttaaatacca aaatttcgat tcatatcttg
     1561 gatttcagta tatatcataa atttgctcaa ctgataaaag tatcaaccat catgaagaac
     1621 gatcatctta tcttaagaat gaattacaag atctggatgg aaactgagga tggtacaccg
     1681 attttgggcg aaggcaagtg taagttactg aaagaaattc aaaggacagg ttctttgaag
     1741 attgccatcg aaaatttagg cttatcctat cgtaaaacat ggaacaacct aaaccggatc
     1801 gagaaaaaaa tcggctttca tctagttgaa aaacaaaggg gtgggaaaga aggcgggcac
     1861 acggctctta cccctcaagg cgaaaaagtt gtagaagcat tcgacaggta ttaccaggaa
     1921 gttgatccac tggttaaaga tttagtgggg aaggtagtat taaaatttcc ccatgcagga
     1981 taacgtggat aaatttcaat tatatccgat tcgtatattt gctgacgaat tgcaatattt
     2041 ttcaactcca aaatctacta aaacttaaaa tgatgaaaca aacaaaaatc acccaagaaa
     2101 agccattgag ctttgacaga ttcaaaaaaa tggttttgaa agcaatagga cggcttggta
     2161 atcctttacc atccatcatg ttagtttgca tggctatttt attagggcca gcccaagttt
     2221 attcacaaaa catcgtcaag ggcaaagtat tggatgcgaa ctcgcatgca gccattcggg
     2281 gtgctaatgt cattgaagaa agcagcaaag ttggaacaac cacaagtacc tcaggcgaat
     2341 tttcgctcga gatttcgaaa gatgttcaaa aaccagttct cattatcagc tatttgggat
     2401 ataaaaccca gaagtttgct gtatatgtcg aggggaaaaa atttatcgaa gtatttctcg
     2461 aaccggaagt ttatatggtg cctgaattaa tcgttttagg gcagcgcctt tcgagcaagc
     2521 aaaacgaaaa cgctgcgagg atctatgttg ttcccgaaga agaaattcat gccatgccaa
     2581 aattgagtgt tgacgagatt ttagcagaac ttccgggcgt agtggtttcg agaggttcag
     2641 gaatcttatc atccagtgct acagtttcga tgcggggaat gggaagcgag caagggcgta
     2701 cactggtttt actcgatgga attccattaa ataaagccga tggaggttca gttcagtgga
     2761 acaacatcga tttgaattcc ataaaacagg tcgaagtctt gaagggaccc ggttcatctt
     2821 attttggtgg taatgcaatg gggggcatta tcagtatggt aaccgccatg cctcaaaaga
     2881 aaatagaagg acaagccgct ctagaatatg gtacgtataa cagcatgggt gcacatttga
     2941 acgttggagg caagacaggg aaattttcgt ggaatgccgc aggtcgtggc cgtaaaagcg
     3001 atggttacat cgttcaacct cccgatgaaa tagattcaac cgtcataaaa accaatatgg
     3061 aagaaattgg ggtaaacctg aaggctcgat acgatattga cacaaaacag tatgttcagg
     3121 tgtcgtattc ttattttgac gacaagagag gaggaggcaa taatttccat cccttcaatg
     3181 aaatggccga acccgacgga acctatactt ctcataccaa caattacata aatgtaaatt
     3241 atggtttcag agctgataaa atccttgccg atgtcagagc atattataat gacgaagatt
     3301 atgttaccat ttcggaaagc cttaagggaa ctcaatttac caaatacgat gtggactcga
     3361 aaagaacgga tatgggcctt atgtcgtcgg ccaccctgcc cttccagaac ggccattcgc
     3421 tcactttcgg tttagacctg aaatcgggaa aagtagatgg caacgatata tacaagactt
     3481 caaccgataa ggtaattaat cagggaacca actttaatag tggtttgttt ttaatgggga
     3541 atattcaact cttaaaggga aaattgatgt taacacctgg cttgcgatgg gattatgccc
     3601 atttttacga tggcgtattc gttgtcgaaa acccaaccaa agttacagca tttctcgtcg
     3661 ggtttcaaga cactatgcct acggcagatt ggaatgcctt tagcccaaga ttgggtttac
     3721 agtataattt caatcccgac acaagaatat atgctcaggc atcgagtggt ttcagacctc
     3781 caattctcga cgacctttgc cgtaccggcc gaatgagact cggactgaaa ctggccaatc
     3841 ccaacctgaa acccgagcgt atttacaact tcgaattagg agccgactat cgttataaat
     3901 tgattactct ctccctttcg ggatattatt cgagaggaac cgacttcatt tattatctgg
     3961 ctaccggcga ctcggccgtt gtcagcaaca aattacgacc tgttttgatt aaggacaaca
     4021 tcagcgacgt tgacatttat ggtctcgagt tcatcaccaa attgcaactt ccggctggtt
     4081 tcaatttgaa agccaattgt gcctatactt ccaccgaaat tggaaattat atcgtaaaat
     4141 ttcccggtcg cgaagaagac cttacaggaa aagaactcgt catggtacca aaattccaag
     4201 tcggggcagg tttgtattac agttacaaat ttgtcaatgc agccatcgtt tatcgcttta
     4261 cagggaaaca atggatcgac gacagcaaca aggaagaatt acaggaaacc aacttgttcg
     4321 atgctaactt gactcttcat cttcatcgct acatagatgc aagcgtagcg gtttataaca
     4381 ttttcgaccg actttattac gatacaaaag gattgatgac tcccggtaga tttatcatgg
     4441 gaagattaac cttcaaatta tagttaatta aaatcattgc aaaatcaatc atctgaaaaa
     4501 tatgaacaag acaatttatt cgagctttat tctcttgttt ttcatttgca tgcaaaactt
     4561 ttgtgttttc agccaaacca acgatttcta tgacgaccag cccacgattc ctttgggcga
     4621 tgttcaaata aaagtagaag gtgaaataga gcaacactac catgtcgatt tcactaaact
     4681 gccacttcgc agtgtaatcg tgaaggaagc cgttttagac tctgaaggcg accattttat
     4741 cggagcatac cgttatgatg gttattctct ttatgacatc ctgaacgaag ctgttctaaa
     4801 aaaggcgaat caggcagaat ttcctcccgt catcgatctg tttgtggaag tggaaggagt
     4861 gaatggtgac aaggccattt ttagttgggg cgagatatat tatcccattc accgacatga
     4921 aatcattatt gctacacaga tttcacgaat tgttccgtca aaaacaaagg attactggcc
     4981 tttacctggt tctccacggg tagttgctgc tgccgatttg cattctcatc gttacattga
     5041 aaaccccaaa agcataaagg tacgttcgtg gaatcgacct tttgttaaac atgaggaagc
     5101 aaaatttacc aataaaattt cccttgaaat cgatggagaa aagaagatgg aaatagttcc
     5161 ctcgagcctg aaaccaccaa taagtaaata ttctactgtt ttttacggca gaggaagagg
     5221 aattcacggc atatccactt tcgaagggaa accattaaaa aatttgcttt ctaccttctt
     5281 taagattaat ccacagatac ttatggaagg attactgata gtgtatggca tcgacggcta
     5341 ccgaagcgtt ttctcctgtg ctgaggtgat ggatagaaac gatcaggaag aaattttgct
     5401 cgtttttaac gacaacctcg ataaccctga agcggccggc ttcagaatct tccctgcctg
     5461 cgactttttc agcgatcgtg cagtgaaggc tatcgaaaaa atcgaagttg tttttaaata
     5521 agaattcaac tcttgaaaga aaaatatcgg attttttaaa atgaatttct caaaactcca
     5581 ttaaatacaa ggcgaataga agaacccaac ctctgaacat ctgaacattt gaatgatgca
     5641 aagtggtgta tagcctgggc tttggctcag gcttttttaa tcttttacta agactattat
     5701 tttattttag ccgtcaacta agcaatgtat tcaatattcg aataagttta ctccattatc
     5761 gctctatccg atgtaatttt gcatttataa aaaattcttt ttcatggcaa tagaaaacga
     5821 aaagtcaaaa cgcaccgaac tatcagaatt gggtgaattt gatttgatcg accggttgac
     5881 cgagggtttg actcccctca accatagcac tataaaaggg gttggagatg atgcagccgt
     5941 tatgcaatat gataatcatt cgcttattct tcttgctaat gacattctaa tagaaggtgt
     6001 acattttgat ttaacttaca ccccattaaa gcacttagga tataaggcgg gagtagtaaa
     6061 tttttcggat atatatgcta tgaatggtgc acctaagcag ttggtcattg gcttgggtgt
     6121 atcgaatcgc ttttcagtag aagctctcga tgagttctat gaaggcttgc gtcttgcttg
     6181 ccaccattat ggagtggatt tggtgggagg cgacatttcg tcgagcaaaa cagggatgtt
     6241 tatttcaatt agtgtagttg gcgaagtttc aaaagatgaa gtggtttacc gcaatggagc
     6301 ccaacttgac aatcttatct gtgtttcggg ggatctggga gcagcttata tgggattatt
     6361 gctcttagaa cgcgaaaaac gggtttatct ggccaatccc aacatgcaac ccgacctcga
     6421 aggcaatgat tatgttttgg aacgtcaact caaaccagag gcacgtaaag acatcattcg
     6481 tcttttgcat gaagcctgta ttcaacctac agccatgatc gacatctcag atggtttagc
     6541 aagcgaaatt cttcatattt gcaagcagtc caataaaggt tgcgtaatct acgaagacaa
     6601 aattcccatc gatccggcta cactactatt atgcgatgag ttcaaaatca ttcctgctat
     6661 tgctgccttg catgggggag aggactatga actgttattt acgattcctc tgtcggagta
     6721 tgaaaaaatc gaaaaagtgg aaaacatctc acttattggc catattaccg attcatcgga
     6781 aggatgccag ttaattacca atgatggcaa acaaatcccc cttcaggctc agggttggaa
     6841 tcatttgaga aaataattcc ttcaaatgtt gcagcatgac aaagagtgac gaacaaatca
     6901 acgagcattt atcgcaaatt ctacaaaccc tccctcatag ccctggagtg tatcagtttt
     6961 ttgatgaaaa tcagaaaatt atctatatcg gtaaggcaaa tgatttgaag aaaagagttt
     7021 cttcatattt tactaaagaa actggggtaa gtggtaaagt attagtgatg gtgaggcgta
     7081 tcagaagcat cgaagtgatt gtggtagata ccgagttgga tgcacttcta ctcgaaaaca
     7141 atctgatcaa ggagcatcag cccaggtata atatcaatct gaaagacgac aagacatatc
     7201 cctggctttg tataaaaaac gagcctttcc cgagaatatt tcctacaaga aatccagtaa
     7261 aagatgggtc gaaatattat ggtccttatg cctcgggacg aatcatgaac aacctcctgg
     7321 agttgattca tcagatttat cctttacgca actgcaacct gaaacttacc tttcgaaata
     7381 tcgaaaaagg aaaatttaag ccctgtcttg agtatcatat cggaaattgc aaagctccct
     7441 gtattggact gcaaaccgaa gaagaatacg atgccaatat tgcttcgatt aaggatatta
     7501 ttcggggaaa cttatcatct gtggcttcgt ctttgaaaag agaaatgtta gcccatgtcg
     7561 aaaatctcga gtttgaaaag gctcaaagag taaaagaaaa attggagctg ctcgaaaaat
     7621 accagtcgaa atcggctgta atcagcaata ctgatttaca tgcagatgtg ttttcgattg
     7681 taagtgacga aaaaatagct tgtgtcaatt ttatgaaagt agccaatgga gcagtcatcc
     7741 agtcgcacac catagaaatt cgcaagaaat tggacgagtc cgatgaggaa atactgagtt
     7801 ttgccattac tgaactcagg ttaagattcg aaagccaggt tcgggaaatt attgttcctt
     7861 tcaaattgac cattgatttt cctggcattc tgattacagt gcccaaaaaa ggagataaaa
     7921 ggcagcttct cgaactttcg ctgtcgaatg ccgaatacta tcgtttggag aaactcaagc
     7981 gcattgaact ggtcgaccct gaaagacata gcaaacgcat tctgcatcaa ataatgaaag
     8041 atttgcgtat gaatcaattg cccgaacata tcgaatgctt cgacaacagc aatttgcaag
     8101 gcactgaagc cgttgctgcc gtggcagtat tccgcaatgc aaaaccctct aaaagcgaat
     8161 atcggcattt caacatcaaa accgtggaag gtcaaaacga cttcgcttcg atagaggaaa
     8221 tcgtttaccg aagatacaaa cgccttttag aagaagggga agggcttccc cagctgattg
     8281 tgatagatgg tgggaaaggg cagcttgcat cggcactctt cagtcttgaa aaattgggac
     8341 tcaaaggcaa gatcaccata ataggtattg ccaaacgttt agaagaaatc tattatcccg
     8401 gagattcgct tccaatgtat ttagataaaa aatcggaaac tctgaggctg ttgcagcata
     8461 tccgcgacga agcccatcgt tttggaatta cacatcatcg caaacgaaga gaaaaggcat
     8521 tcatacagac tgaactcaca agtatcaggg ggattggtga agccacggca aaaaacttat
     8581 taaaagcatt tggctccctc gaaaacataa agaaagccga agctgcagaa atagaaaaag
     8641 tgattggaaa agccaaaaca aaattgcttc ttcaacattt caaccaatca taatattggc
     8701 tttgggataa gcataatcct taaaactggc ctttttcaaa aaggcaatgg ctaaagtcag
     8761 agtgcccact ctgccagcat acattgtaat aataattacc attttactag ctgaggtgag
     8821 aaatggggta attcccattg tgagccctac tgttccaaaa gctgagactt cctcgaataa
     8881 tatgctagtg aatgaaaaat cgggttgtaa aacagtaagg acaaaagttc ctgttacaat
     8941 cagaatcagg gataataaag ccacagaata ggctttatct actgtttcga aagaaatggt
     9001 atgcttgaag gcttccagcc gttttttgcc tcttatggtc gcaatgcttg ctttgatgag
     9061 agttaggaag gtagtggttt ttattccacc tcctgtacct ccgggacttg ctccaataaa
     9121 catcagaatg atgagtagaa acagcgtagc ctggctcaaa gctccaatgt tcaccgtgtt
     9181 ataaccagca gtacgagccg taatggattg aaaaaaaacg gtagttatct tatccgaaag
     9241 ttttaatcca tagagtgaat gattgtattc gagcaggaaa acaccaagag cacccagaat
     9301 gataaggcag acagtagtag ctagtacgat cttagtttgc acctgaaaat gtttccatgc
     9361 cgtccgcaat ttagtcaaaa gcagtttaaa gttaaatatt tcctgaagaa cggagaatcc
     9421 aattccaccc aatacgatta aaatggcaat aatccagtgt gaagccagat tcattcgtac
     9481 cggctcgaac atcagtccat ttggaaatat cgaaaacccc gcattattga atgacgaaac
     9541 cgcatgaaac agggcagcaa aacccactcc tccgggtgat gcaatgctta aatctttctc
     9601 ccagagctga cataaaatga tgctccctgc aatttcaatg gtaaagctat atagaataat
     9661 tcgtttcaag atccggcgag catccgaaag tccgctggta tccaaaagtt ctttcaataa
     9721 tgattgctgt ttcagccctt cggcacccgt ataaaataat gcaaaaaaag tagcaaacga
     9781 aatgatattg attcctccta attgaataag gattaagatc accacctgtc ccttaaaggt
     9841 aaagtaattg gaaatatcca cagtggataa gcctgtaaca caagaagcgc tggtgctgat
     9901 aaataaagca tccacgggtt gaatgtgatg atcgacggtc atttcgggta acaataacat
     9961 agccgtaccg aaagcaatca atataagaaa agacagggca agtaagcctg cagggccaat
    10021 tttgatccta tcgatatatt gacctgcttt ggccgtttcg agcagcacca tgataaaaac
    10081 ataaaactga atgaaaagaa ttgaaagatc ggggaattgt tccattcctc tttgggcaaa
    10141 aaagccatat attctttgtt gacccacaat gagatagaaa aggcccatga ggatgataaa
    10201 taaaaccagt attccttcaa accaggttct tctcaaatag gccaaaggat ggaaatcata
    10261 gaaaaaattc aagaaatatt tcaaagcata aaaagccaaa ctaaagatgg taataattct
    10321 gacaatatca tcggtgtgag gagtacgggg aaatccgtga taataaacta tggcacaaac
    10381 tgcaacaagt gaaactataa tgctgagaat tctcaagacc catagcaggg tttccttatg
    10441 aggataaaga aagatattta ttctttcccg cacacgatct atccaggaat tagacataat
    10501 tctcagtaaa acttaatggt ttaattattc gttgatctga ataaaacggt cgatgtcttt
    10561 atttttacca aataatacaa gcctgtcggt gagtttaatt acatatttcg aatcgggcac
    10621 tcctgccaca tgatatatgg acatctccat attgttcact tttgtggtgg tttcttcctt
    10681 aatggttatc aagcttagtt tataacggtc gcgaagatcg aggtcaccaa gcattcgccc
    10741 ggcaactttt ttaggtgtca gcatttctac gattcgataa tcgtcgggaa gctgcaaata
    10801 cgacactacg gatggattga gcagacgctc ggctacaatg gctcctactt cttcctcggg
    10861 tgaccacaag tcctttacac ctaatttttc gagtattatt ctttggcttt tccctccaaa
    10921 acgtgcaata atacgtttca cgcccaagtc gagtaataac gccacacaaa gcaatctttg
    10981 ctcgaaactc tcccctatgg aaactactac tgcatcaaat tcctgtatat tctgactcaa
    11041 aagtgccttt ttatctgttg catcaatagc cactgccaga gaaacatcat ccgaaacacg
    11101 ttcgatgttt tcctgaagct tatcaatagc cagtacctct gcacccttct tcgacagatt
    11161 cttcgcaatg gattccccga accggcctat gcctataacg gctattttcc tatgtaccat
    11221 ataaatgaat ttagatggct tgcaatttgg aggcgcaaat atatttcaat tttatgcatt
    11281 ttacacattc cagaccaaac tcgaaaaata ttcataaaaa atttcggtgt ttttgtgata
    11341 tggcttacag ctttaaacaa aagttttaaa aaatggcatt aagcctgctg gcatggtaaa
    11401 cttaaatcat gcctttaaat ggaaaggagc agatcggttt ttccagaaat ttattgagct
    11461 cgagcaaagc caaaacccat ccttacatta aagcagatga tgattacgat aaatcgaaag
    11521 gtgaaatctt caacttcatt ctgcatttta tatatcgata ttacgatcgt tagagatatt
    11581 ttagccacct cttttcgttg taataaattt actgccacag catatcatcg agtacataac
    11641 aggcaaagct tgaggtaaac gaggccgaac ataattgaat attgcgcact tgtcataatt
    11701 ttgcgccgga tgaatctttt tgaagaaatg aaacctttta tcggaatagt tttttgtgtc
    11761 ttttccattc ttccttccag aattttcgct aaggatcaga attggactac ttactgcgaa
    11821 tcgactgatt ttaggagaac gccgcgatat aaagaaacca ttcatttttg taaacagctt
    11881 gccgaaaatt cccatatcat tgcttatgaa gaatttggaa ttagtcctca gggccgagca
    11941 ctccctcttc tcattatgga taaggataga aatttttcgc cagaactagc ccgtcgggct
    12001 ggaaaggcca ttttgatgat tcaggcatgt attcatcctg gagaacccga agggaaagat
    12061 gctggattga ttctgtttcg caacttaggt attttaggga tggatacttc tttactccgg
    12121 catgttacct tggttttcat cccgattttc aatgtagacg ggcatgagag gtttagccct
    12181 tatcatcgta tcaatcaaaa tgggcccgaa gagatgggct ggcgtaccac ggctcaaaat
    12241 ctcaatctca accgagattt tatgaaggct gatgcccccg aaatgcaggc ttggctgcga
    12301 ttattcaact attggaatcc cgattttttc gtcgatacac acactaccga tggtgccgac
    12361 tatcaatatg tcttgaccta ttcacttgaa accggaccca ataccgattc ttctctgggt
    12421 aaatatctcc gggaaaatta tattccaact atggaagcct acatgagcga aaaaggatac
    12481 aaagtctttc cttatgtgaa tttcaaacaa tggcatgatc cacgctcggc gttatactct
    12541 ttgccggctt cggctatgtt atcgcacgga tatgtcgcag cccgcaatcg ccccgctctg
    12601 ctcatcgaaa cccacatgct gaaaccctat aaattacgag tatcgagcac cctcgaactc
    12661 ttatttttta cattgaaaca tctgaacgat cactatcgcg actatttgca aataaaccgc
    12721 caagccgata cgtatacatc ttcggccgat ttccgtccgt ttcctctgaa attcaatttt
    12781 ataaccagca ataccgatag tgtgatggta gatttcgaag gttatgacta cagaattgaa
    12841 aagagtgatt taaccggtgg aaactggttt atctacttac ctcgacctaa aacatttcgc
    12901 ttgccaaatt tcaaccatgc catagctcag gattctatac tgctgcccga ttattacatc
    12961 attccgcccg agtggactac aatcatcgaa agacttcggc ttcatggaat tgaaatgaat
    13021 tttttaacct acgacacaat tcttaaagtt gaatcatatc gcttcagcaa tcctcaatgg
    13081 gcaaacttcc cattcgaagg gcggaacagg ctttctgtaa aaacggaaaa atatgtcgaa
    13141 agacgagctt acccggctgg cagcgcactg attagtcttc agcagccgac tgctcgcatt
    13201 atagctctct tgctcgaacc ccaatcttcc gattcattcc ttacctgggg tttcttcaat
    13261 gccattttcg aacagaaaga atacgccgaa acttatgtta tggaaaaaat agccaggcaa
    13321 atgattgaaa aacgacctga cttattgcct cagtttcaag cttggaaaga aaaaaaccct
    13381 caaatggata aagatcaatg gatgcagtta aactggtttt atcagcatag tccctatttc
    13441 gatcaaaacc tgaataagta ccctgttggc cgaatttttc tttaccaata agtatttaag
    13501 ccctcaaacc ttcaaaactc aacctattgt tttcgagaac actacagaga aaacctttca
    13561 ggatttcgag tttatttggt gccttgatgg gccgcatcat gtcttttcat ttcatagctt
    13621 cattaatacc tcgaatcctg catcatttac aatttaataa actgttttat atcagtaatt
    13681 tatgtaagaa tagaataagg ttataaggtt taagagtagg cttttatcta ttttgttcta
    13741 ataaggttgc cgaattcaaa ttacaaatta caatctctcc gtctctcggt ctcatcgtca
    13801 cttatgtcaa actcggaaaa cttttttctc gctgattgac gcagatttaa tccgctgatt
    13861 tacgctgata tttttctgtg tgctcctgcg aaagcttttt tctgcgatat ccttagacat
    13921 cgacacacac acactaatca ccatccacta tccactatct aacagtcccc ttgtcccctc
    13981 gtctcccagt ctctccgtcc cctcgtcccc tcgtcccctc gtcccctcgt ctcccttctt
    14041 ccgtttttaa ctttataaac tattaacttt caacttttta ctcaataacc ccaataccta
    14101 aaaccattaa caatgcgcaa tatattatct caaaaaaata tttttcattt ttttactaaa
    14161 agcttaatga atagaaaaat tatccttaca tttggacttt taaactgaat gagttctttg
    14221 ataaatataa aaacatgata gtactataaa acataaaaga atatttagag tctgtctata
    14281 aactccattt agagcatctg cggtttccat cattaaaaac gaaaatgaac aaactactat
    14341 ttttcgacca tttcttataa aaaacacaaa agtaataatt tatcgaatga atgtttaatg
    14401 tttttgaaat aattctctca ataattattt gtattatagc attaacataa tttgtcaaat
    14461 gataagattt aaagtcattt tgttaacgaa ccaaatttta gaagattaga ttcttttccg
    14521 ttgttctaaa aaatgtcttt aactaaaaaa tcttcaaggg gaaacatcaa aattatccca
    14581 atcgggtgcg atgaagaatt tttcgcgaaa acgtttcagc tcacccaaat cgatattagc
    14641 cgacaaaaca ccttccttgg caggttccat ttgagcaata atattcccct tggggttaat
    14701 aatcatagaa ggagctgaat acgaaaggcc ctcaccatct tgtccacaac gatttatgcc
    14761 caccaaataa gcctgatttt ctatagcacg cgcaattaat aattgttccc aaacacatcc
    14821 tcgtggatct ggccaattgg catgataaat caaaatatca taagcaaact ggaaatttga
    14881 atatcggttg cgactccaaa caggaaatcg aaggtcataa catatctgcg gctgaatctt
    14941 cgcattttta tattcaacaa tcacattttt attgccaggg gtatataatt ggtgttcact
    15001 ccctaaactg aaaagatggc gcttgttata actggccata acaccctctc tagatacata
    15061 aagagcccta ttgtagtatt ttccatgatc tataatggga atagtgcctg ccagtataca
    15121 attcatttct tttgctattt tctgcatcca tgaaacagta ggtccattca tgggttcagc
    15181 caaaccgata tttttaactg aaaatcccgt agtaaaaaat tctggtaata aaatcaaatc
    15241 agtttcatta gggagacttg taagtaaatt ttctacttta tgcctattca ttaaaggctc
    15301 ctcccatttc aaatctaatt gcattacggc aatattcatc atggatattt cctttagagg
    15361 tttgtatgtg agatatcttg gggcaaactt acataaaaaa ataattttta tacgccgcac
    15421 aataaaaatc aaggccataa ttgttctata agttatcaac gtactcaaaa ggtacaattg
    15481 tttaacacta ctgaccgaca aaaaaattgg ataaaaagaa ggagggacaa aaccgaagtt
    15541 tca
//
LOCUS       DAOORU010000041        15434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4683_length_15434_cov_5.571429, whole genome shotgun sequence.
ACCESSION   DAOORU010000041 DAOORU010000000
VERSION     DAOORU010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15434
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4683_length_15434_cov_5.571429"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1384
                     /locus_tag="PLF20_04155"
     CDS             <1..1384
                     /locus_tag="PLF20_04155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HOX80235.1"
                     /translation="IDQSSPSLIKDDSKCIRCQRCVRTCSDLQAVSALSAAYKGSEMK
                     ISSFYDRPMHEVICTNCGQCINRCPTGALTERNYIDEVWDAIYDPGKHVVVQTAPAVR
                     VALGEELGYDPGARVTGKLVTALRRLGFDSVLDTDFTADLTIMEEGTELLTRLKKVLV
                     DKDTSMALPMFTSCSPGWIKFIEHTFPDLLPHLSTCKSPQQMFGALAKTYYAQKKGIN
                     PKDIVSVSIMPCTAKKFEATRPEMRDSGYQDVDYVLTTRELAIMIKQAGIEFMKLDDE
                     DYDRFMGESSGAGVIFGATGGVMEAALRTAYEIVTGREVPFQNLNITPVRGMEGIKEA
                     EVKIENVKPDWSFLEGVTLRVAIAHGLANAKRLMTAIRDGKVVYHFIEIMACPGGCLG
                     GGGQPIPTNLEIRKKRAEAIYAEDENLPIRKSHENPEIIQIYQEFLGEPLSHTSHELL
                     HTHYVPRKRY"
     gene            1490..1882
                     /locus_tag="PLF20_04160"
     CDS             1490..1882
                     /locus_tag="PLF20_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX80236.1"
                     /translation="MKDIKILLVDDDIDFVFIQKAFLEKEGFQVITAGDKFAGFERAR
                     AEKPDLAILDVMMTTDQEGFEMAREMRKDPQLKDIPIIMLTSVDSVMGLNLKEMANDP
                     KWLPVDSYLEKPVQPQKLLDEIKRLLKL"
     gene            2470..3618
                     /locus_tag="PLF20_04165"
     CDS             2470..3618
                     /locus_tag="PLF20_04165"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HOX80237.1"
                     /translation="MKYKFWFVIICCSFFVFQGLAQTNISLVSGKNVKPEKNGVFFSL
                     PRKIIKVSVSVREIENLRGPYAEYAQQLMGISNPVTQNTKEYEIADISMEGLVEPDPE
                     RIYFLEVDDKLSKEEKNFSVCLTSNGIIRSINGISSSKEAQLIKEEKKVIGQEDLFSM
                     SMSPSLYRKVDTIIRMVTVDTNTVKKRFFNTYFEEKPTEIKAREAADMVSRIRESRFN
                     LLTGYQETSYSPETMKYMDEKLQLMAQEYISLFKGLKTENLHTYTYYIVPDQNKSNQV
                     ICKFSKETGISDATDSKGKEIVLSIKRSGTMIQMPAWASAPVEVKSLLVPYRIPEMVR
                     LSVNYVGKVYDEQVIALPQFGVVSVVPFVKSKIEFDPQTGCIVKIYYE"
     gene            complement(3609..4436)
                     /gene="kdsA"
                     /locus_tag="PLF20_04170"
     CDS             complement(3609..4436)
                     /gene="kdsA"
                     /locus_tag="PLF20_04170"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOX80238.1"
                     /translation="MIPDGLPLLRHKNSDNFFLIAGPCIVENPQMPLEIASVIYDICE
                     RLEIPFIFKASYKKANRTSIHSFTGIGDILALKAIADVRNELRIPVLTDFHTAEEAEL
                     AAAYVDVLQVPAFLCRQTELLVAAALTQKVINVKKGQFISPEAMQFVVEKLKSQGNNR
                     IILTERGTTFGYNDLIVDFRSIPIMQSFDVPVVVDITHSLQQPNTSAGVSGGSPQFIS
                     TIGKAAVAASADGIFIETHPEPSRALSDGANMLQLSLLENLLKQLVQIRKALNRNYS"
     gene            complement(4452..5717)
                     /locus_tag="PLF20_04175"
     CDS             complement(4452..5717)
                     /locus_tag="PLF20_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOX80239.1"
                     /translation="MTPPLAEILRPQSLSEFIGQEHIIQPNSILYNSIQAGKVPSMIL
                     WGPPGTGKTTLAYIISRTLNCHFFSLSAIHSGVKEVRTVIEEAEKNPSRTILFIDEIH
                     RFNKSQQDSLLGAVEKGIITLIGATTENPSFEVITPLLSRCQVYILKPLERKHLEILI
                     EKAVRYYDTQGIHLQIAENEALLRISGGDARKLLNAIELIVNNRKTGTNSLLIDNGIT
                     QKIIQQNMAIYDKGGEQHYDIVSAFIKSLRGSDPNAAVYWLARMIEGGEDPLFIARRM
                     LILAAEDIGIANPNALLLANTCFDAVHKIGWPESRIILSQTAIYLATCPKSNSAYMAI
                     EKAQEAVKSSGDVSVPLHLRNAPTQLMKQLGYSKGYQYAHDFPDNFVAMDFLPSSLQG
                     NIFYEPGNNPRENEIKNRLKILWKNKYKY"
     gene            complement(5724..6350)
                     /locus_tag="PLF20_04180"
     CDS             complement(5724..6350)
                     /locus_tag="PLF20_04180"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA-like putative outer membrane lipoprotein
                     chaperone"
                     /protein_id="HOX80240.1"
                     /translation="MKKYLILLLAFTPVFVQAQYSSEKAQNQLQSLAQTLKSAQNIRI
                     QFTLSQTADKASASKPNQGTLWISGNKFRLMLDGQIIVNDGKTLFTYLPEANEIQIND
                     ANSSHNELNPLTLLTSYQQNYRSKYIREEDNRMIIDLLPLNENRFYKIRIEILTSNNY
                     PSKISLYERNGTTLTYQIDRFELNKDVNSSIFSFNPADYKNAEVVDLR"
     gene            complement(6369..8906)
                     /locus_tag="PLF20_04185"
     CDS             complement(6369..8906)
                     /locus_tag="PLF20_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005775772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOX80241.1"
                     /translation="MAAKVNHKTEKEGIEEKGKPSNKPHKKKQKSTAWWRQKEKSRAV
                     IGFIITAIGIYLLLAFISFFINWHIDFDKTTNISFYDIASGKTNFKNTTGSIGAAISY
                     YIIYKGFGLASFVFPFIFILIGLRIALGIRIFPLHKSFSISFFILIWLSVTLGFAFHS
                     TNNLSNILGGVFGNQISRYMMVVMGKTGLVILLLALLLVFTTIYMNFPFSQIQNYRRR
                     KQEEKKNKLQPSPSTVPAPSVSYNLHEFAVDEPATTESKIIDKPSPKKAENNSPSNPQ
                     PNSKSAREEVEEGTPGAQPKNVDKPVSSHDEVELIIVNSKNKEQTNKEPEITEEKGNP
                     ITHYTLDTLYDPTLELSDFKLPTADLLQTYPNENANVYKEEIEDNKKRIVETLNNYQI
                     DIQQIKATIGPAVTLYEIIPAPGIRISKIKNLEDDIALSLSALGIRIIAPIPGKGTIG
                     IEVPNLNPEIVSMRSLITSEKFITTRFDLPIALGKTISNECYIADLSRMPHLLMAGAT
                     GQGKSVGLNVIITSLLYKKHPAEVKFILVDPKKVELSLFSKIERHYLAKLPDCEEAII
                     TDIRQVVRTLNSLCIEMDDRYALMKSAQVRTIKEYNHKFIHRRLDPNEGHHYMPYIVL
                     IVDEFADLIMTAGKEIELPLTRLAQLARAIGIHLIIATQRPSVNIITGTIKANFPARI
                     AFRVSSKIDSRTILDTGGADQLVGRGDMLLSLGSDLLRLQCAFVDLSEIESITGYIGS
                     QRGYPEAYALPEYYESEDEIPAITEPGERDELFEEAARIIVIHQQGSTSLLQRKLKLG
                     YNRAGRLIDQLEAAGIVGPFEGSKAREVRIPNEQALEQYLRELNNKQ"
     gene            9339..9806
                     /locus_tag="PLF20_04190"
     CDS             9339..9806
                     /locus_tag="PLF20_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOX80242.1"
                     /translation="MEEQLIQEVIDLAFQNVMEGKGGPFAAMVVRGGHIIAIATNTVT
                     STNDPTAHAEVNAIRQACKQLNTFQLDDCELYTSCEPCPMCFGAIYWARLRKVYYAAR
                     QEDAAQAGFDDSFIFEQLTLPSSERKIPFVCMHHHEAIKPFVLWNRMEEKIKY"
     gene            9828..10220
                     /locus_tag="PLF20_04195"
     CDS             9828..10220
                     /locus_tag="PLF20_04195"
                     /inference="COORDINATES: protein motif:HMM:NF022769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HOX80243.1"
                     /translation="MALTLKGKVVKILPIQQGEGRNGVWKKQSFIIETFGYYPRKICL
                     IAWNERTDVVSSLEIDEEVSVECSIESREFNDRWYTDVRALNISKSTPSSISSSSDSE
                     EIHSQPESPSEPPAIEYNEIQDDNDLPF"
     gene            10344..11162
                     /locus_tag="PLF20_04200"
     CDS             10344..11162
                     /locus_tag="PLF20_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOX80244.1"
                     /translation="MDDKITEATQIDENIFSQGLTVYEVIRVIKGKLLFLEDHLKRFE
                     NSARNMGFQLSLSQKEITYRLYEMLHANGSLDGNIELDVNYRPDSFSPKQTFVAWYIP
                     HHYPTEEEYRQGVKTGLFYYERKDPNIKYRNIDLKEKVKIFIEKNKIYEALLVDDGGA
                     VTEGSKSNVFFIKENQFFTPPLSKVLPGVTRQKIIYLINQQNIVLNEGNIYVKTLNTY
                     EASFLTGTSPKVLPIAFINEQSYNVNHPLLHFIMKSYDDIIESYLNEGINGLNQ"
     gene            11180..12085
                     /gene="ppk2"
                     /locus_tag="PLF20_04205"
     CDS             11180..12085
                     /gene="ppk2"
                     /locus_tag="PLF20_04205"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF015909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOX80245.1"
                     /translation="MGNNSNCNELNELFGEEFRKYIQPRDESEQIKFGEDADNGHEVS
                     KLGKKFYEKELRKLQIELVKLQEWVKAKGLRVVVIFEGRDAAGKGGVIKRITECLNPR
                     ICRIAALPVPTEKERTQWYFQRYVSYLPSAGEMVLFDRSWYNRAGVERVMGFCTEEEY
                     WEFFRSCPLFEEMLIRSGIILIKYWFSVSDHEQEQRFQQRLADPTKRWKLSPMDIASR
                     EKWEDYSKAKDEMFAFTDTKISPWWDVNADDKRRARLNCISHLLSMIPYEELPSPLIK
                     FSPRKESTGYVRPPIKRQNFVPERY"
     gene            12104..13942
                     /locus_tag="PLF20_04210"
     CDS             12104..13942
                     /locus_tag="PLF20_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOX80246.1"
                     /translation="MKKLLIFMFLLSLIVANPVKQLLAQSPPSWTSNVIWYQIFVERF
                     YNGNPNNDPTPEDISVSSSTFPLPDDWTITPWTMDWWKREPWAEKTGKSHRETVQHRR
                     YGGDLQGVIDKLPYLHELGITAIYFNPLNDAPSLHKYDARNYHHIDVNFGPDPKGDKK
                     IIASEIPSDPSTWKWTAADRQFLQLIEQCHQMGIRVIMDYSWNHTGVDFWAWKDVLQN
                     LQKSPYRDWYEIISFDNPLTPENEFQYRGWANVASLPEWKKVNLPGPRKSNGPYEGNL
                     NPKVKEHIFAVTQRWLAPNGDKTKGIDGFRLDVAEQIPLGFWRDYRKWVKSIKPEAYL
                     VGEIWWEKWPDKLMDPAPYVNDEIFDAVMFYKIYAPARSFFVHGKEALTSSQLADSLQ
                     KEWTNLPKNFRYSQMNVNATHDSPRLLTCFANKGKYKYHVNPYENPRCITGKPDKDTY
                     NRVRLYLMHQFTIPGSPSIWNGDEMGMWGSDDPDNRKPLWWPDTKFEPETRTNIQPGF
                     KLYDEVKFDADHYHFYKQIISLRKNNPVLATGEIEFISSEDRVLAYRRWNSSDEIFVC
                     LNTDTTSHRIKFASKGKFVDLLSGRIIKGKSIPILPLEGLILRRVE"
     gene            complement(14159..14893)
                     /locus_tag="PLF20_04215"
     CDS             complement(14159..14893)
                     /locus_tag="PLF20_04215"
                     /inference="COORDINATES: protein motif:HMM:NF040157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5714 domain-containing protein"
                     /protein_id="HOX80247.1"
                     /translation="MSDNKNGCLICGEDLVYNDILEKTMCFYCKKIFETNVICKNQHY
                     VCDGCHSLPAYSAIETFCLMNMLKDPLEIAILLMQHPSIKMHGPEHHFLVAASLLTAY
                     YNTINEPSKKSVILTEAQKRAKNILGGFCGYYGNCGAAVGTGIFLSLITQATPLSKKE
                     WELCNLMTSNSLFSIAHAGGPRCCKRNTYLAVIEAARFVNENFQISLKTGKSLQCSFS
                     SFNRECKKEDCKFYKGSRINVLIEKN"
BASE COUNT         4648 a         2820 c         3259 g         4707 t
ORIGIN      
        1 tatcgatcaa tcttcgcctt cgctcataaa ggatgatagc aagtgcatcc gctgtcagcg
       61 ctgcgtgcgc acgtgttctg acttacaggc tgttagcgct ttatcggcag cttacaaagg
      121 ctcggaaatg aagatttcga gtttctacga ccgacctatg catgaagtga tttgtaccaa
      181 ttgtgggcaa tgcatcaatc gttgcccaac gggtgctctt accgaacgta attatatcga
      241 cgaggtatgg gatgccatct atgatccagg taaacatgtg gtggttcaaa ctgctcctgc
      301 cgtgcgtgta gctctgggag aagaacttgg ttacgatccg ggtgcacgcg ttacaggaaa
      361 acttgttaca gctcttcgta gactgggatt cgattcggtg ttggataccg attttacggc
      421 tgatttaacg attatggaag aaggaacaga actgcttact cgtttgaaaa aagtattggt
      481 tgataaggat acatcgatgg ctctacctat gtttacttcc tgttcgcctg gatggatcaa
      541 gtttatagaa catactttcc ccgaccttct gcctcatctt tcaacctgca aatctcccca
      601 gcagatgttt ggcgcattgg ctaaaactta ctatgctcaa aagaaaggga ttaatcccaa
      661 ggacattgta tcagtttcca ttatgccctg tacagccaag aaattcgagg ctacgaggcc
      721 cgaaatgcgc gatagtggct atcaggatgt agattatgtc ttaaccaccc gcgagctggc
      781 tattatgata aaacaagccg gcatagaatt tatgaagctc gatgacgaag attatgatcg
      841 ttttatgggt gaatcatcgg gagctggtgt tatttttgga gcaactggtg gagtgatgga
      901 ggcagctttg cgtacggctt acgaaattgt cacaggtcgt gaagtcccat ttcaaaattt
      961 gaatattacc cctgtaaggg gcatggaagg cattaaagaa gccgaggtca aaatcgaaaa
     1021 tgtaaaacct gactggagtt tcctcgaagg agttacgttg agggttgcta ttgctcatgg
     1081 tttggccaat gccaagaggc tgatgactgc tatacgtgat ggcaaggttg tttatcattt
     1141 tatcgaaatc atggcatgtc ctggaggatg cctgggtggt ggtggccaac cgataccaac
     1201 caatctggaa atcagaaaga aacgtgcaga ggcaatttat gcagaagatg aaaacctgcc
     1261 tattcgtaaa tcccacgaaa atcctgaaat tattcaaatc tatcaggaat ttttgggtga
     1321 acccttgagc catacatcgc atgaattact gcatacgcat tatgttcctc gaaaacgtta
     1381 ttaatttaga caataactgg atttagtaat gaagtatcgt gaaatttctt aattttatcg
     1441 agaaaataag aattcaacaa aagtcgaaac atttaaaatt tcagtcatta tgaaagacat
     1501 taaaatattg ttggtagatg atgatataga ttttgtattt atccagaaag cttttttaga
     1561 gaaagaaggt tttcaggtga ttacggcagg tgataagttt gcaggctttg aaagagcaag
     1621 agccgagaaa cccgatctgg ccatactcga cgtgatgatg actactgatc aggaagggtt
     1681 tgaaatggca cgcgaaatgc gtaaagatcc ccagctgaaa gatattccca tcatcatgtt
     1741 gacaagtgtg gattcagtta tgggtctcaa cttgaaagaa atggccaatg atcccaaatg
     1801 gcttcccgtg gattcctacc tcgaaaaacc tgtccaaccg caaaaactcc tggacgaaat
     1861 aaaacgcttg ctcaagcttt gaaaagctta tatttcaaat tcaatagccg gtctgcttag
     1921 gccggctatt gaatgttacg cgcatactca gattgtccct ttctcactac gttgccttag
     1981 attatgatct tttgctcaga atagcaagca tgcacgttga ccaagctgga tataataaag
     2041 caatcaactg agccgctttc actatgtata gctccgtgta tatctttgga aacctttgtt
     2101 ttatgtaaaa tcgaagaata ggattatatc aaaacgggat ctgattcaaa cggatttatc
     2161 atatagtttt actatttcta ttattttgag aataatgatt ctatttctga agtggagaat
     2221 caaaataaat tttatccgat gattggaata aaaataagga atgtatgtat ttaaacgctc
     2281 tgtttcttat ttaatacatg agtttggaag tgtatccggg taatgtccga cttatattga
     2341 atagctaaat gtgaatattt aaaattatcg aacaaataca acaaatcaac gaaagaatta
     2401 attttgtgaa aacttctcgt agacgaagaa gaaaatttgt tgaaatttat tccaaaaaaa
     2461 ttgatcgata tgaaatataa attttggttt gttataattt gctgttcttt tttcgttttt
     2521 cagggtttgg cacagaccaa catcagtttg gtgagcggta aaaatgtgaa gcccgagaaa
     2581 aatggagtat ttttttcctt accacggaaa atcatcaagg tttcggtaag tgttagagag
     2641 atagaaaatt tgagagggcc ttatgccgaa tatgcccagc agctcatggg cataagcaat
     2701 cctgttactc aaaatacaaa ggaatatgaa attgccgata taagtatgga gggccttgta
     2761 gaacctgatc ccgaaaggat ttacttcctt gaggtggatg ataaattgag caaagaggag
     2821 aagaattttt cggtatgttt gacttctaac ggaatcattc gtagtattaa tggaatttct
     2881 tcgtctaaag aagctcaact catcaaagaa gaaaaaaaag tcattggtca ggaagattta
     2941 ttttccatga gtatgagtcc ttcgctttac aggaaagtcg ataccatcat aagaatggtt
     3001 accgtagata ccaatacggt taagaagcgt tttttcaata catatttcga agaaaaaccc
     3061 acagaaatta aagctcgaga agcagccgac atggtgtcaa gaattcgtga aagccggttt
     3121 aatttgttga caggttatca ggaaaccagt tattcgcctg aaactatgaa gtatatggat
     3181 gaaaaacttc aattaatggc tcaggaatat atttctcttt tcaagggatt gaaaacagag
     3241 aacctgcaca cctataccta ttatattgta cccgatcaga ataaatcgaa tcaggtcatt
     3301 tgtaaatttt cgaaggaaac aggtatttcc gatgccaccg atagcaaagg gaaagaaatc
     3361 gtacttagca taaaaagaag cggaactatg attcaaatgc ctgcctgggc ttctgcaccc
     3421 gttgaggtaa aatctctttt agttccttat cgtattcctg aaatggtgag gttatcagta
     3481 aattatgttg gaaaggttta tgatgaacaa gtgatagctc tccctcaatt tggtgtagtt
     3541 tcggttgtgc cttttgtaaa gagcaaaata gaattcgatc cccaaacagg atgcattgta
     3601 aaaatctact atgaataatt tctgttcaag gcttttctga tttgaacgag ctgtttcaag
     3661 agattttcga gcagggagag ttgcagcata ttggctccgt ctgataaagc tctggatggt
     3721 tcaggatggg tttcgatgaa aataccgtca gcgcttgcgg caacagctgc ttttccaata
     3781 gtagaaataa attgaggcga gcctccgctt acgccagctg aggtgttcgg ctgctgcaac
     3841 gaatgcgtta tgtcaactac aacgggcaca tcaaaacttt gcatgatggg aatggagcga
     3901 aaatcaacta tcaaatcgtt ataaccaaag gtagttcctc tttcggtaag gattattcga
     3961 ttattaccct ggcttttgag cttttcgacg acaaattgca tggcttcggg cgaaatgaac
     4021 tgccctttct ttacgttgat tactttttgc gtaagtgcgg ctgcaacgag taattctgtt
     4081 tgccggcaaa gaaatgcagg aacctgcaat acatctacat aagcagcagc cagttcggct
     4141 tcctcggccg tatgaaaatc ggttagaact ggaatcctta attcattacg cacgtcggcg
     4201 atggctttca aagccaaaat atctcctatt cccgtgaagg aatgtatgct tgtgcgattg
     4261 gcctttttat aggaagcttt gaaaatgaaa ggtatttcga gacgttcaca gatatcatag
     4321 attactgagg caatctcgag gggcatttgt ggattttcga caatgcaagg ccctgctatc
     4381 aggaagaaat tatcggaatt cttatgccgg agcaagggta aaccatcagg tatcataatt
     4441 ttaattgggt ttcaatattt atatttgttt ttccagagaa ttttaagacg atttttaatt
     4501 tcattttcac gagggttatt gccaggttcg taaaatatat ttccttgcag cgaagaaggg
     4561 aggaaatcca tagcaacgaa gttatctggg aaatcatggg catattggta acccttgctg
     4621 tatccaagtt gtttcatcaa ttgagtggga gcattgcgta agtgaagcgg aaccgaaaca
     4681 tcgccacttg atttcactgc ttcctgggct ttttcgatgg ccatataggc cgaattgctt
     4741 tttggacatg tagccagata aatagcagtt tgcgacaata tgattcgact ttcaggccat
     4801 ccaattttgt gaactgcatc gaagcaagtg ttggcaagta aaagagcgtt gggatttgca
     4861 ataccaatat cttcggcggc taaaatgagc attcggcggg cgataaataa aggatcttca
     4921 cctccctcga tcatgcgtgc cagccaataa actgctgcat tggggtcact accacgcaat
     4981 gacttaataa aggcggatac aatatcgtaa tgctgctcgc ctcccttgtc gtaaatggcc
     5041 atgttttgct gaatgatttt ttgagtgatt ccattgtcga tcaaaaggct gttggtacca
     5101 gtttttcgat tattaacgat cagttcgatt gcattcaaca acttacgggc atctccgccc
     5161 gatatcctta atagcgcttc attttcagca atttgtaagt gaattccctg agtgtcgtaa
     5221 taccgaactg ccttttcgat taaaatttcc agatgttttc tttccaacgg cttcagaata
     5281 tagacctgac atctcgaaag caaaggtgta atgacttcga aggaaggatt ttcggtagtg
     5341 gctcctatta gcgtgataat gcccttttcg accgctccta acaaagaatc ctgctgcgat
     5401 ttgttaaaac gatgtatttc atcaataaac aatatggttc tgcttgggtt tttttcggct
     5461 tcttcgatta ctgtacgaac ctcctttact cccgaatgga tagcgcttaa cgagaaaaag
     5521 tggcagttca atgttctcga aatgatataa gcaagtgtgg ttttacctgt gcctggaggg
     5581 ccccacaaaa tcatcgaggg cactttccct gcttgaatag aattatatag aatggaattg
     5641 ggctggataa tatgttcttg gccgatgaat tcggaaagcg attgtggacg caggatttcg
     5701 gcaaggggag gagtgatcat ggtttatcgc aaatcgacta cttctgcatt cttataatcg
     5761 gcagggttaa atgaaaagat ggaagaattg acatccttgt tcaattcgaa tctatcgatc
     5821 tgataagtga gagtagttcc attacgctca taaagcgaaa ttttcgatgg atagttattg
     5881 gatgtcagaa tttcgatcct gattttgtaa aacctatttt cgttcaaggg caataaatcg
     5941 atgatcattc ggttatcttc ttcacgaatg tattttgatc tgtagttttg ttgatagctt
     6001 gtcaagagcg tcaatggatt caattcatta tgggacgagt tggcatcatt gatctgaatt
     6061 tcgttggctt cgggcaaata ggtaaatagg gttttcccat cattaacgat gatctgacca
     6121 tccagcatca ggcgaaattt gtttcccgaa atccagagtg ttccctgatt tggttttgac
     6181 gcactggctt tatcagcggt ttgcgaaaga gtgaattgta ttcggatgtt ttgagccgat
     6241 ttaagagttt gagctaagga ttgaagctga ttttgagcct tttcgctact atattgggcc
     6301 tgaacaaata caggtgtaaa agcaaggaga aggatgaggt attttttcat gttttaatag
     6361 attgtgcatt attgcttatt gttgagttct ctcaaatatt gttccaacgc ttgttcgtta
     6421 ggaattctta cttcgcgggc cttacttcct tcgaaagggc ctacaatgcc agcggcttcg
     6481 agttggtcaa tcaagcggcc tgcccggtta tatccgagtt ttaattttct ttggagcaag
     6541 gaagtactac cttgctgatg gatgactatg attctggcgg cttcttcgaa gagttcgtct
     6601 cgttcgcccg gctcggtaat agctggtatt tcatcttcac tttcgtaata ttcagggagg
     6661 gcataggctt cgggatagcc tctttggctg cctatgtatc cagtaatact ctcgatttcc
     6721 gataaatcga caaaagcaca ttgcaaacga agcaaatcac tcccaagtga aagcaacata
     6781 tctccccgac ccactaattg gtcggctcct cccgtatcaa gtatagttcg tgaatcgatc
     6841 ttcgaactca ctctaaatgc gatgcgagcc ggaaaattgg ccttaatggt gcccgtgatg
     6901 atatttaccg aaggacgttg agtggcaatg atgaggtgaa taccgatggc ccgggctaat
     6961 tgagccaaac gagtgagggg taattcgatt tctttgcctg ccgtcatgat caaatcggca
     7021 aattcgtcta caataaggac gatgtatggc atgtaatgat gtccttcgtt tggatccaat
     7081 cgtctgtgga tgaatttatg attgtattcc ttgatggttc ttacctgagc agatttcatc
     7141 aaggcatagc ggtcgtccat ctcgatgcat aagctgttga gtgtgcgtac gacctgacgg
     7201 atatcggtaa tgatggcttc ttcacaatcg ggtaatttgg ccaaataatg acgctcgatt
     7261 ttcgaaaaga gagaaagttc tactttttta ggatcgacca ggataaattt tacctctgca
     7321 ggatgtttct tatacagaag acttgtaata ataacattca agccaaccga tttgccttgt
     7381 ccggtagctc ctgccatcaa taaatgtggc attcggctca aatcggcaat atagcactcg
     7441 ttggaaatag ttttgcccaa tgcaatgggc aggtcgaaac gcgtagtgat aaatttttcg
     7501 ctagttatca ggctgcgcat cgaaacgatt tcagggttca gattaggaac ctctatgcct
     7561 atggtgcctt ttcccggaat gggagctata atcctgattc ccaaggccga tagacttaag
     7621 gcgatatcat cttctaggtt cttgatcttc gagatacgga ttcctggagc agggataatt
     7681 tcatagaggg tgacggctgg cccgatcgta gcttttattt gttgaatatc gatctgataa
     7741 ttattgagcg tttcaacaat acgtttcttg ttgtcttcga tttcttcctt gtaaacattg
     7801 gcattttcgt tgggatatgt ttgcaaaaga tcggccgtag gaagtttaaa atcggaaagt
     7861 tctaaggttg gatcataaag cgtatcgaga gtgtaatgag taatggggtt tcccttttct
     7921 tcagttattt ctggttcttt gttggtttgc tccttattct tggaattcac tataatcaat
     7981 tcgacttcat catggctgct tactggctta tccacgtttt taggttgagc gccaggtgtt
     8041 ccttcttcaa cttcttcccg agcagatttg gaatttggct gcgggttgga tggagaattg
     8101 ttttctgctt tttttgggct gggtttatcg attatctttg attctgtagt tgcaggctca
     8161 tccaccgcaa actcatgaag gttataactt acagaaggtg ctggaacggt cgaaggagag
     8221 ggttgcaatt tatttttctt ttcttcctgc ttgcgacgcc tgtagttttg aatctgcgaa
     8281 aaagggaaat tcatgtaaat ggtagtaaat accagtaaca gcgccagtaa aagtatgacc
     8341 aatccggttt ttcccattac aaccatcata taccgactta tttgattgcc gaatacccct
     8401 cctaaaatat tagacaggtt gttggtacta tgaaaggcaa aacccagagt taccgataac
     8461 cagattaaaa tgaaaaatga aatactaaag gatttgtgga gagggaaaat tctaatacct
     8521 aaagcaatac gtaatccaat cagaataaaa ataaatggga aaacaaaact ggccaaacca
     8581 aatcctttgt aaataatgta ataggaaatt gccgcaccta tcgaacctgt cgtgtttttg
     8641 aaattggttt tccccgatgc aatgtcataa aatgaaatat tggtagtctt atcgaaatcg
     8701 atgtgccagt tgatgaaaaa tgaaataaaa gctagaagca aataaatgcc tattgctgtt
     8761 attataaaac cgataactgc tcttgatttt tctttttggc gccaccatgc cgtagatttt
     8821 tgttttttct tatgcggttt gttagaaggt tttcccttct cttcaatccc ttctttctcc
     8881 gttttatgat tcactttcgc tgccatccta tctccgaatc aggttgatta ttgcaaaaat
     8941 aatcaatttt aactttcgtc ttttcggcat ttgaaatgtt ctttctttgc taaggtcgtg
     9001 ttatctcgtt cgaagccttt cttctcttcg aatatctgcg aaaaaacttt tcggcttatg
     9061 attagatcga tatttttggt acgttagcca tggcattatc ttcacgaaga ttgataaaaa
     9121 cctgaaatat aagacttcga gtaatttcat gaataatgac tttatttgat tttcaaccat
     9181 ttgccaactg gaatattttt gttggagatg cgatggtaaa cgttttcatc gatttctcat
     9241 ccctgcagaa tattttgtta aatgaatctg tttttttgaa tgatgttcga aaattctcgt
     9301 gtatttttga aagaagaaac gaatgaatac atgattagat ggaagaacaa ttgatacagg
     9361 aagttattga tttggccttt cagaatgtaa tggaagggaa aggaggtcca ttcgcggcta
     9421 tggttgtacg aggcggccac ataatagcca ttgccaccaa tacagtgact tccacgaatg
     9481 atccaacggc tcatgccgag gtgaatgcca taaggcaagc ctgcaagcaa ctaaatacct
     9541 tccagctcga cgattgcgag ctgtatacca gttgtgagcc ttgtccgatg tgttttggag
     9601 ctatttactg ggctcggctt aggaaagttt attatgctgc ccggcaggaa gatgcagctc
     9661 aggcaggttt cgatgattcg tttatttttg aacagctaac tttgccatct tcggaaagaa
     9721 aaataccttt cgtttgcatg catcatcacg aagccataaa accctttgta ctatggaatc
     9781 gaatggaaga aaagatcaaa tattgaaatt taaaaatata ttcagctatg gcgctaacac
     9841 ttaagggaaa agtcgtaaaa attttgccga ttcaacaagg cgaaggtaga aatggggtat
     9901 ggaagaaaca gagtttcatc atcgaaacct tcggttatta tcccagaaaa atatgcttga
     9961 tagcatggaa tgaacgcaca gatgtagttt cgagccttga gatcgacgaa gaagtttccg
    10021 tggaatgctc gatagagtct cgtgaattca acgatcgttg gtacaccgat gtaagagcct
    10081 tgaatatttc gaaaagtacc ccttcttcta tttcatcttc atcagactcg gaagaaatac
    10141 attcacagcc tgaatctccc tctgaacctc cggctataga atataatgag attcaagatg
    10201 acaatgattt accattttaa agatataccc caatgctttt ctaatgatat gatcattgaa
    10261 tttctcggga gtacaggatt aagaatctaa acataacatc taatttttcg aaaatgatta
    10321 ttccgttgag aaaatatatt tggatggatg ataaaatcac cgaagccact caaattgatg
    10381 aaaatatctt ttcacaagga cttacggttt atgaagtcat cagggttatt aaaggcaaac
    10441 ttctatttct ggaagatcat ttgaaaagat tcgagaattc tgctcgaaac atgggatttc
    10501 agctttcatt gagccaaaag gaaattacat atcgacttta tgaaatgttg catgccaatg
    10561 gttccctcga tggcaatatt gaactcgatg tgaattaccg gcctgatagt ttttccccaa
    10621 aacaaacttt cgtagcatgg tatattcctc accattaccc tacggaagaa gaataccgtc
    10681 aaggagtaaa aaccgggctg ttctattatg aacgaaagga tcccaatatc aagtatagaa
    10741 atatagattt gaaggaaaag gtaaaaattt ttatcgaaaa gaataaaata tatgaagctt
    10801 tacttgtgga tgatggcgga gcagttacag aagggagcaa gtcgaatgtg ttctttataa
    10861 aagaaaatca attttttacg ccaccacttt cgaaagtact gcctggtgtt actcgtcaaa
    10921 aaatcattta cctaatcaat caacaaaata ttgttttaaa tgagggaaat atttatgtaa
    10981 aaacgctgaa tacttatgag gcttcttttt taactggaac ttcaccaaaa gtactaccca
    11041 ttgcatttat caatgaacaa tcatataatg taaatcatcc gcttttgcat ttcattatga
    11101 aatcttatga cgacatcatc gaaagttatc tcaatgaggg gataaatggt ttgaatcaat
    11161 gatttaaaat caataattta tgggaaacaa ttccaactgt aatgaattga atgaattgtt
    11221 tggggaagaa tttcggaaat atattcagcc tcgagatgaa tctgaacaaa taaaatttgg
    11281 agaagacgct gataatggcc atgaagtttc gaaattagga aagaaattct atgaaaaaga
    11341 attacggaaa ttacagatag aactggtcaa attacaggaa tgggtaaagg cgaagggatt
    11401 gagggtagtg gttattttcg aaggtcgtga tgcagcagga aagggtggcg ttatcaagcg
    11461 tattaccgaa tgtctaaatc cacgtatttg tcgaattgca gccttgccag ttccaaccga
    11521 aaaggaacgc acacagtggt attttcagag atatgtttct tatttgccca gtgcgggcga
    11581 gatggttctt ttcgatcgct catggtacaa cagggcaggt gtggaacgtg tgatgggttt
    11641 ttgtactgaa gaagagtatt gggaattttt ccgttcatgc cctcttttcg aagaaatgct
    11701 cattcgtagt ggaattattc ttattaaata ttggttttcg gtaagtgatc atgaacagga
    11761 gcaaaggttt cagcaacgtc ttgccgatcc gactaaacgt tggaaactca gtccaatgga
    11821 catcgcctcg cgcgaaaaat gggaagatta tagcaaagca aaagatgaga tgtttgcctt
    11881 taccgacacg aaaatatctc cctggtggga cgtgaatgcc gatgataaac gcagagcccg
    11941 tctcaattgc atctctcatt tactaagcat gatcccttat gaagaattac cttcacccct
    12001 catcaaattt tcacctcgaa aagaatcaac gggttatgtt agacctccaa taaagaggca
    12061 aaattttgta ccagaacgat attaagaatt ttttgcaatg aaaatgaaaa agttattaat
    12121 ttttatgttt ttattgagtt tgattgttgc aaatcccgta aagcaacttc tggcacaaag
    12181 ccctccttca tggacttcca atgtgatttg gtatcaaatt ttcgtagaac gcttttacaa
    12241 tggaaaccct aataatgatc ctactcccga agacatctcg gtgagctcgt caacctttcc
    12301 cctccccgat gactggacaa ttacgccatg gacgatggac tggtggaagc gagaaccttg
    12361 ggcagaaaaa acgggaaaat cccatcggga aaccgttcag catcgccgtt atggaggcga
    12421 tctgcaaggc gttatcgata aacttcccta tctgcatgaa ttgggaatta ccgctattta
    12481 tttcaatcca ctaaatgatg caccttcttt gcataaatat gatgcgcgca attaccacca
    12541 tatagatgtg aattttggtc ccgatcctaa gggtgacaaa aaaattattg cttccgagat
    12601 cccatctgat ccttctactt ggaaatggac ggcagccgat aggcaattcc tccaattaat
    12661 cgaacaatgc caccaaatgg gcataagagt cattatggat tattcgtgga atcatacagg
    12721 tgtggatttc tgggcatgga aagatgttct gcaaaattta cagaaatcac cttaccgtga
    12781 ttggtatgag atcatttctt tcgataaccc ccttactcct gagaatgagt tccaatatcg
    12841 tggttgggct aatgtggcca gcctcccgga gtggaaaaaa gttaacttac ctgggccaag
    12901 aaaatccaat ggtccctacg aaggaaattt gaatcctaag gtaaaggaac atatttttgc
    12961 cgttactcag cgatggcttg ctcctaatgg agataaaacc aagggaatag atggttttcg
    13021 cctcgacgtg gccgaacaaa ttcctctggg tttttggaga gattaccgta aatgggtaaa
    13081 atctattaaa cccgaggctt accttgtggg agaaatctgg tgggaaaaat ggcccgataa
    13141 gcttatggat cctgcacctt atgtaaatga tgagattttc gacgcagtaa tgttctataa
    13201 gatttatgca ccggctcgta gcttcttcgt tcacggaaaa gaagccttaa cttcttcgca
    13261 attggccgat agtttacaaa aagaatggac aaatctgccg aaaaatttcc gttactctca
    13321 aatgaatgta aatgctacac acgatagtcc acgtctgctg acttgttttg ccaacaaagg
    13381 aaaatacaaa tatcatgtca atccttacga aaatcctcga tgcataacag gtaaacccga
    13441 caaagatacc tacaatagag ttagattgta tctcatgcat caatttacta ttcccggttc
    13501 tccttctatt tggaatggtg atgaaatggg aatgtggggc agcgacgatc ccgataatcg
    13561 taaaccttta tggtggcccg acacgaaatt cgaacctgaa actcgaacca atattcagcc
    13621 tggctttaaa ttatacgatg aagtgaaatt cgatgccgat cattatcact tttataagca
    13681 aatcatcagt ttacggaaaa ataaccctgt tctggctacg ggcgagattg aatttatttc
    13741 ttcagaagat cgagtactgg catatagacg ctggaattct tcagatgaaa tatttgtttg
    13801 tttgaatacc gataccactt ctcatcgcat caagtttgca tcaaaaggga aatttgtgga
    13861 tttactttct ggaagaatca ttaaaggcaa atcaattcct attttgcctc ttgaaggatt
    13921 gattcttcgg agggtggagt aagttaatat gatgcaagtc cccaggttcg aggagacctt
    13981 taaattcttg aggcgacacc atgaggagac gaagagacgc aaaatttacg taatagatag
    14041 tactgatttt tcaaaaagtc agtagttttc tttgtaattt tggtggtatg ccatttattg
    14101 ttgattttca ctcgttttgt ttcggttaag aaacatttta ataaaaagat gaaaaaggtt
    14161 aatttttctc aattagaaca ttgatgcggg aacccttata gaacttacag tcttcttttt
    14221 tgcattctcg attgaacgaa gagaatgaac actgaagaga ttttcctgtt ttgagtgaaa
    14281 tttggaaatt ctcatttaca aaacgagcag cctcgataac agccagatag gtgttgcgtt
    14341 tgcagcagcg aggacctccc gcatgagcaa tcgaaaaaag actgttggat gtcatcaggt
    14401 tgcaaagttc ccattctttt ttggataaag gagttgcctg ggtaatcaag cttaaaaaaa
    14461 tgccggtacc cacggcagca ccacaattac cataatatcc acaaaaacct ccaaggatat
    14521 ttttagcacg tttttgggct tcggtgagta taaccgactt ttttgacggt tcattgatgg
    14581 tattatagta agctgtaagc aaagatgctg ccacaagaaa gtgatgttct ggtccatgca
    14641 ttttaataga tggatgttgc atcaataaaa tagctatctc gagtggatct ttcagcatat
    14701 tcatcaagca aaatgtttcg atcgctgaat aggctggcaa actatgacat ccgtcacaaa
    14761 cataatgctg gtttttgcaa attacgttgg tttcgaaaat tttcttgcaa tagaagcaca
    14821 tggttttttc gagtatatca ttgtaaacta agtcttctcc acaaatcaaa cagccatttt
    14881 tattatccga catgatatct cgagagatgc aatttattat tgagcgaaat taataaattt
    14941 atttattcaa tattgacttt aagcttgact atcttttttt tcttctacca aagctggccg
    15001 aaaaattcga attaatgaag aaaattcaga taagatatgc aaataaatta atttttgtag
    15061 tttcattctg attttttatg gggattactt ggtaaacttt agttttccat gaatgatgca
    15121 aagggctatt tttatactat agtagaattt tttaaattat ttatcattac tgaccgacaa
    15181 aaaaattgaa taaaaagaag gagggacaaa accgaagttt catctctaat actgtatcaa
    15241 taaaacataa ttgaggatca tagaaccaat caggttgttc gaataaaatc ttaaaatctt
    15301 caaataaacg catggcgtta tttttttaat aatttccaac aaaattagaa taaaaaataa
    15361 tgccaataac agttttaatt aattagttat caaaaagtta ttaacaattt gagtttatag
    15421 acagactcta acta
//
LOCUS       DAOORU010000042        14747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_4975_length_14747_cov_5.692213, whole genome shotgun sequence.
ACCESSION   DAOORU010000042 DAOORU010000000
VERSION     DAOORU010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14747
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4975_length_14747_cov_5.692213"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..1340
                     /locus_tag="PLF20_04220"
     CDS             339..1340
                     /locus_tag="PLF20_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system membrane protein
                     PorP/SprF"
                     /protein_id="HOX80248.1"
                     /translation="MRRKSYIFSMILVLSIIGIDMKGQDPTFSQFYANPLYLNPSLTG
                     SAFSPRLTLNYRNQWPSLADNFVTYNASYDQYVPDINSSFGIIFNTDRAGSGMITTTT
                     VSGLYSYNLKISRNSYINAGLQGSYYQRKLDWLNLTFGDMFHPVYGQIYPTSEPSPDK
                     YSISFLDFSAGLTYTYRDVFFGGLSVNHLTQPYDGWYNDNSTVLPRKWTLHAGGIFDL
                     EPRTRGRRSMEAMTLSPNVLYQQQQNFKQLNVGLYLNKYPFVVGTWFRHNFENPDAFI
                     FLLGFQQSSFKLGYSYDITVSKLKNVTGGAHEISFTFQFTSPEQSRRKNEPMPCPSF"
     gene            1387..3018
                     /gene="gldJ"
                     /locus_tag="PLF20_04225"
     CDS             1387..3018
                     /gene="gldJ"
                     /locus_tag="PLF20_04225"
                     /inference="COORDINATES: protein motif:HMM:TIGR03524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldJ"
                     /protein_id="HOX80249.1"
                     /translation="MVKFYKIAALFVIVILVAGFSSCSMFRKNKEQSTATGWNYNDPT
                     QGGFEKFDFKGQPNGPGLILIEGGTFVMGATQDNVMGEWNNTPRRVTVRTFYMDQTEV
                     SNLHYLEFLYWVKKVFYQTNNQQYQEYYVKQLPDTLVWRDRLAYNEPMVNLYLRHPAY
                     RDYPVVGVSWLQANEYCRWRTDRVNEMLLYKAGVYHLDTDYDVNDFFTTESYLGGFFT
                     KGDTLKGDTLGLPDLSTNKKGAKRRVRMEDGIVLPNYRLPTEAEWEFAALGLIGNTEF
                     ENIKERRIYPWNGNFTRINSPKEADYGQFAANFRRGHGDYMGVAGSLNDAADIPAPVI
                     SYYPNDYGLYNMAGNVAEWVLDVYRPSSFEDFSELEPFRGNIFTAPELAGQGKFQIDR
                     EVIKPDGTPNENFGRFKRRPVDSQIDPTLPFRRNYRTADNRNYLDGDWASLSQASNVD
                     AAQRWKEVGNNEQATNKMYYNGEWNPAENKFGEGATSLITDRSRVYKGGSWKDGAYFL
                     SPSVRRFLEENLSTDYIGFRCAMDRVGTTSENIEY"
     gene            3683..4972
                     /gene="murF"
                     /locus_tag="PLF20_04230"
     CDS             3683..4972
                     /gene="murF"
                     /locus_tag="PLF20_04230"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HOX80250.1"
                     /translation="MENMEELYRLYLSSKGICTDSRKLEVGQLFFALRGENFDGNDFA
                     EIALENGAIAVVIDDPEKIALRNFYYVPDALTTLQQLAQFHRKHFQIPVIAITGSNGK
                     TTTKELISSVLSGKYRIKATSGNLNNHIGVPLTLLSIQADTEFAIIEMGANHLGEITF
                     LCEIVQPDYGLITNIGKAHLEGFGNLEGVIRAKTELYKFLALSNGLAFVNGKDELLLT
                     KSSNLRRILYNSDSSAVKGDIVDTTGYLKVSLRILNRDYLLHTQLVGAYNLPNILAAA
                     CIGNYFGVEDDKICEAIKNYRPSNFRSQFIKTQHNKLVMDAYNANPSSMEAAITNFVS
                     RFESPKAAILGEMLELGLESKKEHQAIVDKIASANIQKLFFVGKNFSDIALPPTAMHF
                     ENATLLKDYLRKEPLQQYFILIKGSRGNKLETLLDVL"
     gene            4962..6044
                     /locus_tag="PLF20_04235"
     CDS             4962..6044
                     /locus_tag="PLF20_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOX80251.1"
                     /translation="MFYNGKNHYKGLGMMSGTSLDGVDLAACDFNFENDNWNFEILQA
                     ETIPYPSFWVDRLNRVMEASAAELASLNMDLGKYYGKIAFEFLNHHKLEVDYIASHGH
                     TVFHDAEKGFTFQAGNGVAIAAATHQPVICDFRTADIVRGGQGAPLVPIGDELLFNSY
                     SACINLGGFANISYNKDGKRIAFDLCPANMALNFLAQKANLPFDYNGELAASGRIIQP
                     LLFELLHLEYFSKNPPKSLGKEWFLQSFLPILNKYEGEQIQDLSRTVCELIAIVIMRS
                     LFDVQPKEKGVLITGGGAHHHSLLQRIKELSTLPIAIPSPCIIDFKEALVFAFLGLLF
                     LEQKPNILSSATGAETDSIGGCLYLP"
     gene            6230..6850
                     /locus_tag="PLF20_04240"
     CDS             6230..6850
                     /locus_tag="PLF20_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80252.1"
                     /translation="MDSKLQELTERIYHEGVEKARADAAAILGKANKEAEEILQNARH
                     EASRIIEQARREAEELKRNVTNEIKLSSRQAISSLRQQITELILTEMVDQSVKEAFND
                     KDFIKRMIETILTRWQPDNSGKGQSVQLFLPPEDEEKLGKYFEARAKELMNKGLKVIY
                     DEKIGRGFRIGPADGSYVISFTEEDFENFFKNYLRPRTNHLLYGGK"
     gene            6850..7674
                     /locus_tag="PLF20_04245"
     CDS             6850..7674
                     /locus_tag="PLF20_04245"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOX80253.1"
                     /translation="MSGRNYYYLVAGLPDLVLEQSKLALDFAEFKLELRHHLSAFDYK
                     LVELILLPIDNKNLLHLLLKTNEEFIPNGKYSLEILEEEMKNPKILPDYLRNFILHFK
                     EEQPVYPELSWEDQLTAIYLEYAAQTKNKFVSNWIKFERNVRNIMAALTARKFKLNAR
                     SFIIGNDEFSQTLIKSQARDFGLAQQFPYIEKLIQIFETNNLLEREKSLDLLRWSYLD
                     ELNTFHYFSIEIILAFILKFQMVQRWLRLDKEAGLAMFRRLLEDLENSYEFPKEFS"
     gene            7677..9449
                     /locus_tag="PLF20_04250"
     CDS             7677..9449
                     /locus_tag="PLF20_04250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005638776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOX80254.1"
                     /translation="MNNTKKTTGKVSGIIANLVIVQVEGPVAQNEICYISHDGTDLMA
                     EVIKIIGNKAYTQVFESTRGLKIGSPAIFEGHMLEATLGPGILSSVYDGLMHDLHEMT
                     GIFLKPGEYTRALDPEKIWDFKPLAKGGDQVIAADWLGEVIENSIPHRIMVPFVFEGR
                     YTIKSIVPRGLHKIYDTIAVLTDESGKDHPVTMVQYWPVKQAIKAYKEKPRPFKLFQT
                     GVRTMDTLNPMAEGGTGFIPGPFGSGKTVLQHALSRQAEADIVIVAACGERANEVVEL
                     FVEFPQLEDPRTGRKLIERTTIIANTSNMPVAAREASVYTAMTIAEYYRSMGLKILLL
                     ADSTSRWAQALREMSNRMEELPGPDAFPMDLPAIISNFYSRAGYVYLNNNQTGSITFI
                     GTVSPAGGNLKEPVTESTKKVARAFYALSQQRADSKRYPAIDPIESYSKYLEYPEVQE
                     SLRNTIADDWVDKVMLVKNIMIRGREASEQINILGDDSVPLDYHLTFWKSEVIDSIIL
                     QQDAFDKIDFNTPMTRQKYMLDKVINIYETNFDFDSFEDVNPYFKRIINQLKQMNYSE
                     FQSEQFKHFENELYKIIEERKLKK"
     gene            9446..10768
                     /locus_tag="PLF20_04255"
     CDS             9446..10768
                     /locus_tag="PLF20_04255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOX80255.1"
                     /translation="MSPLAFQKIYTHISNITKATCTLKASGVGNEELALVHGRLSQVV
                     KIIDNEVTLQVFGGTEGIPSNAEVIFYNRPPTLKVSDDLAGRFFNAYGQPIDGGPEVE
                     GEERPIGGPSVNPVRRRQPSELIATGIAGIDLNNTLVTGQKIPFFADPDQPFNQVMAM
                     VALRAEADKIILGGMGLTNDDYLYYKNVFENAGALDRIISFVNTTEDPPVERLLVPDM
                     ALTAAEYFAVDKNEKVLVLLTDMTLYADALSIVSNRMDQIPSKDSMPGSLYSDLAKIY
                     EKAVQFPSGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLRRDTDIGKVIVDPFR
                     SLSRLKQLVIGKKTREDHPQVMNTAVRLYADAANAKTKLENGFDLTDYDQRALDFARE
                     YSEYLLAIDVNIDIDKMLDTGWELFARHFNQAEVGIKQELVDKYWPVK"
     gene            10832..11434
                     /locus_tag="PLF20_04260"
     CDS             10832..11434
                     /locus_tag="PLF20_04260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOX80256.1"
                     /translation="MAVKFQYNKTSLQEMNKQLRIRLRALPTLKNKESALRAEVKRAK
                     DYATSLDEKLSAAMAEYNEMVRLWNEFNPGLVVIHDVDLGIKKIAGVRIPILQQIHFE
                     IKPFSLFNQPVWYMDGILILEKLASIAIEREFYYAKMKLLDHARKKTTQKVNLYEKVQ
                     IPGYEEAVIKIKRFLEDEENLSKSSQKILKNRFDQKEVAV"
     gene            11443..13236
                     /locus_tag="PLF20_04265"
     CDS             11443..13236
                     /locus_tag="PLF20_04265"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOX80257.1"
                     /translation="MTKFIFLVYHEDYERFLKGLRSVGVIDIVKRKKELDKDAAERLA
                     LQKQVAETIKLLKKHKKPEITAEAPSEGDGMKIFEHIRDLQAEVESLNQQLATLKKEI
                     EYTEPWGDFDPQIIEKLRKAGLRISFYIIPSRKFDPQWLSKYNIGVVNEQPGVIYFIL
                     VNEGQEEIDLPAEEVKAPERPLSELLKKQEAIQTRLSEIEEEFARLAASSIPLLQKTL
                     HQIASEVDYHVVVNNTLSEAEGKLKILEGFAPVETKETVEKYCEENGIFYIETQPDPN
                     EKVPILLRNNSFAKLFEPIGHLYSLPKYGELDLTPFFAPFFMMFFGFCLGDAGYGLFI
                     FIAATLFKKFKAKPSFKPYVTLAQYLSLATFIFGAISGTFFGISLIDADIPIVENIKS
                     YFFDSNKMLVLSLILGAVQIVFGMCIKVVNITHQEGFRNALSLVGWLILILGLLIRQG
                     LVSAGVLPKGETIVLYSILGVSCLLIFFLNDLKTNVFVRIGKGLWDVYGMATGIFGDL
                     LSYIRLFALGISSSILGIVINNVAMSILGIPYIGPLLFVVFLVIAHLGNIAISSLGAF
                     VHPMRLTFVEFYKNAGFKGGGKQYKPFAEKR"
     gene            13300..13761
                     /locus_tag="PLF20_04270"
     CDS             13300..13761
                     /locus_tag="PLF20_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOX80258.1"
                     /translation="MTTAVILAYVGLAMMLGLAGIGSAIGVTICGNATIGALKKNEEA
                     FGPYMLLSALPGTQGLYGFGGFFIINAKLTPQFIASMTLGQGMAIFAAGLVLGGVGLY
                     SAIRQGQVCANGIAGIGSGYDVFGKTMVLAVFPELYAIISFAATFIISAAL"
     gene            13843..14208
                     /locus_tag="PLF20_04275"
     CDS             13843..14208
                     /locus_tag="PLF20_04275"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOX80259.1"
                     /translation="MEIKPTVVLGVSRNPERMANQAVEALLNHGHPVYAIGKEDFIWG
                     KIVVHSTWDQAVDIHTVSIYLRPELQKVHQQRILSVNPQRIIFNPGTYNPELAIAASE
                     RGIEILEECTLVLLSSGQF"
     gene            14294..>14747
                     /locus_tag="PLF20_04280"
     CDS             14294..>14747
                     /locus_tag="PLF20_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOX80260.1"
                     /translation="MKRILIILFLTCCALRLPAQILISFKTDSTCKTDTIIVPLKVQE
                     LRQCASFRIEFSFQDDIVHYDSLYFRNPALAGLTAFLNGNNRLVLEWTSASALPFGTG
                     NIAKIQFTALQSGTSLLSFDAAQTYFKDEQGNPLTYLYNDGILKVHPRG"
BASE COUNT         4451 a         2969 c         2965 g         4362 t
ORIGIN      
        1 aactttcgaa aatgagtcca cacacacaaa caaaaatcaa taggttgaat cttaatgtca
       61 tgggcaaata agtttgggaa agtatccctt cgaaagttta aacaaaatcg agacaaaatt
      121 gtgcatttcc acctcaaaat tttatcgatc gaaagaaatt cttctctaaa aatctttcca
      181 gtagggatac tattcaggtt ttttaacgtt ctttacagag ccttattgtt gaaaaaaagt
      241 ttgaattttt tttaacaaat gatgcttata aaaaaaattc gtaatattgt tggatcgttt
      301 tgtgtaccga agaaatcact ttttatatca taaagattat gagaaggaaa agctacatct
      361 ttagcatgat tctggttctc agtataatag gtattgacat gaagggccaa gatccgacgt
      421 tttcacaatt ttatgccaat cctttgtatc tcaatccctc tttgactggt tcggccttca
      481 gtccaaggct aactttgaat tatcggaatc agtggccatc tttggctgat aattttgtaa
      541 cctataatgc ctcttacgat caatatgttc ccgacatcaa tagcagcttc ggtattattt
      601 tcaataccga cagagctgga tcgggcatga taaccactac aacggtgagt ggcctttatt
      661 cgtacaacct gaaaatatcg cgaaattcat acattaatgc aggcttacag gggagttact
      721 atcagcgcaa gctcgattgg ttgaatctga catttggaga tatgtttcat ccggtatatg
      781 gccagatata tcccacaagt gagccctcac ctgataaata cagcatttcc tttctcgatt
      841 tttctgcagg tttgacctat acctaccgag acgtattttt tggtggctta tcggtaaatc
      901 accttacaca accttacgac ggctggtata acgacaacag tactgtatta ccccgcaaat
      961 ggacgcttca tgccggggga atcttcgatc ttgaaccccg cacccgcggc cgaagaagta
     1021 tggaagccat gacgctttca cctaatgttc tgtaccaaca gcaacaaaat ttcaaacagc
     1081 tcaatgtagg attatacctc aataaatacc cattcgttgt tggaacatgg tttcgacaca
     1141 atttcgaaaa ccccgatgcg tttattttcc tgttaggatt tcaacaaagt tcgtttaaac
     1201 tggggtatag ttatgatatc accgtttcaa aactcaaaaa tgttacagga ggagctcatg
     1261 agatttcgtt tacttttcaa ttcactagtc ctgagcaaag cagaaggaaa aacgaaccta
     1321 tgccgtgtcc aagtttttaa tctaattttg taaccctatc aaaaaaagaa gaaaatcatt
     1381 ccaaaaatgg ttaagtttta taaaattgca gctttattcg tcattgtgat cctagtggct
     1441 ggattttcat cgtgtagtat gtttcgtaaa aataaggaac aatctacagc aactggctgg
     1501 aattacaacg acccaaccca aggtggattc gaaaaattcg attttaaagg gcaacccaat
     1561 ggcccgggcc ttattctgat cgaaggtgga accttcgtca tgggtgctac tcaggataat
     1621 gtaatgggcg aatggaacaa taccccgcga cgagttaccg tccgtacttt ctatatggac
     1681 caaaccgaag taagtaacct tcattacctc gaatttctct actgggtgaa aaaagtcttt
     1741 tatcaaacca ataatcaaca gtatcaggag tattatgtaa agcaactgcc tgatactctg
     1801 gtttggcgcg accgactggc ttataacgaa cctatggtaa acctttacct gcgtcatcct
     1861 gcctatcgcg attatcctgt ggtaggtgtt agttggctgc aggccaatga atattgccgc
     1921 tggcgaaccg accgggtaaa cgaaatgttg ctttataaag cgggcgtcta tcatctcgat
     1981 accgattacg atgttaatga cttttttact actgaatctt atctgggagg tttctttact
     2041 aaaggagata ctttgaaagg agatacactg ggtttacccg atctcagtac gaacaagaaa
     2101 ggagctaaac gcagggtaag aatggaagat ggcatcgttt tgcccaacta ccgcctcccc
     2161 acggaagccg aatgggaatt tgccgcttta ggcctcatcg gaaatacgga attcgaaaat
     2221 atcaaagaga gaagaattta tccttggaac ggaaatttta cccgcattaa ttctcccaaa
     2281 gaagccgatt atgggcaatt tgcagccaat ttccgccgtg gacatggcga ttatatgggt
     2341 gttgccggaa gtctcaacga cgcagccgat attcccgctc ctgtcatctc ttactatcct
     2401 aatgactacg gactctacaa catggctggc aatgtagccg aatgggtgct cgatgtttat
     2461 cgtccgtctt cttttgaaga ctttagcgaa ctggaacctt tccgtggaaa tatctttacc
     2521 gctcctgaac tggctggcca gggcaagttc cagatagata gagaagttat caaacccgat
     2581 ggaaccccca atgagaactt tggacgtttt aaacgccgcc ctgtggattc tcaaatcgat
     2641 cctacattac cattccgtcg taattatcgt accgccgaca atcgaaatta cctcgatggc
     2701 gactgggctt cattgagcca agcttccaat gtggatgcag cacagcgatg gaaagaagtc
     2761 ggaaataatg aacaggccac caacaaaatg tattataatg gagaatggaa tcctgccgaa
     2821 aataaattcg gagaaggtgc tacaagcctc atcaccgacc gttcacgagt ctataaaggc
     2881 ggatcgtgga aggatggagc ctattttctc agcccaagcg tgcgccgctt cctcgaagaa
     2941 aacctcagca ccgactacat aggcttccgc tgcgctatgg atagagtcgg aacaacttcg
     3001 gaaaacatcg aatattaatg caataattat ccattgagat gattcctcat ctgtaatgct
     3061 gctgattttt tctgcatcat taaatgccat gatataattt catggcattt ttttattgga
     3121 taaaacgaat gttaatgaca aaaaatgtga tgcatatcat cccccttccc tgaatgttct
     3181 tccctcggaa aacaatttac tctctaatca ttccatgttt aaatttttaa attcatgctt
     3241 gagaattttc aacataaatt atttattaac aaaaacattc caacggccat ttatatggtg
     3301 ccgtttgtag aacttaaaaa attctgaatg ctgaaaaatt tcttctaacc aattaacatt
     3361 tcctgatgga aagttttata cccgtaaata actccagtaa attgctcaaa cttaaaccga
     3421 atgttaatca gctcaaattc accataagta actaaatttc attattttat gagttaatca
     3481 tctttaaaat ttaacctttc atgaattact cttaaatgaa taaatcaaat ccaacaaaaa
     3541 caatttcttt gaagattaaa ataaattcat caaccttttt tattttgagc agcttaacat
     3601 tccaatatca tttctctttt aaataattta tcattattct aaacatcata ttcatccaat
     3661 gtattctaaa tatatttgcg gcatggaaaa catggaagaa ctctatcgct tatatctgtc
     3721 ttcgaagggt atttgtaccg actcgagaaa attggaagtg ggtcaattgt tttttgcctt
     3781 gaggggagaa aacttcgatg gtaacgattt tgccgaaatt gctcttgaaa atggtgccat
     3841 tgctgtagtt atagatgatc ccgaaaaaat agctcttcga aatttttatt atgttcccga
     3901 tgccttaacc accttacaac aacttgcaca gtttcaccgc aagcattttc agattccagt
     3961 aattgccatt acgggttcta atgggaaaac aaccaccaaa gaattgattt cgagtgttct
     4021 atcgggaaaa tatcggataa aggctacctc cgggaattta aataatcaca ttggggttcc
     4081 attgacttta ttatccattc aagccgacac agaatttgcc atcatcgaaa tgggagcaaa
     4141 tcatttaggc gaaattacct ttttatgtga gatagtgcag cccgattatg gccttatcac
     4201 gaatattggc aaggcacatc tggaaggatt tgggaattta gaaggggtta ttagggcaaa
     4261 aaccgaatta tataaatttt tagccctatc caatggattg gcttttgtaa acggcaagga
     4321 tgaactgctt ttgaccaaaa gcagcaatct tcgtcgaata ttgtacaatt ctgactcaag
     4381 cgctgtaaaa ggagatatag tagataccac aggttatctg aaagtgagcc tgagaatttt
     4441 aaacagggat tatttgctgc acacccagtt agttggtgcc tacaatcttc caaatatcct
     4501 cgcggcagct tgcattggaa attattttgg agtggaagac gataagatat gtgaggccat
     4561 caagaattat cgtcctagta atttccgttc tcagttcatc aaaactcaac acaataagtt
     4621 agtgatggat gcctataatg ccaatccttc gagtatggag gctgccatca ctaatttcgt
     4681 ctctcgcttc gagtcgccta aggcagcaat tttgggtgaa atgcttgaac tgggattgga
     4741 atcaaagaaa gaacatcagg caatagtaga taagattgct tcggctaaca ttcagaaatt
     4801 attttttgtg ggaaagaatt tctcggatat agccttgcca cccacagcaa tgcatttcga
     4861 aaatgcaaca ctcttgaaag attatttaag aaaagaaccc cttcaacaat attttatact
     4921 tattaaagga agtagaggaa ataaattaga aaccttactc gatgttttat aacggcaaaa
     4981 atcattacaa aggtctggga atgatgtcgg gtacatcctt ggatggcgtt gacctggcag
     5041 cctgtgattt taattttgag aatgacaact ggaatttcga gattttacag gccgaaacca
     5101 taccatatcc ttctttctgg gtcgatcgcc tgaatcgggt aatggaagcc tctgctgccg
     5161 aacttgccag tctgaatatg gatctgggaa agtattatgg aaaaatagcc tttgaatttt
     5221 tgaatcatca taaattagaa gttgattata tagcctctca tggtcatacc gtctttcatg
     5281 atgcagaaaa aggctttact tttcaggccg gtaatggagt agccattgca gcggccactc
     5341 atcaaccggt aatttgtgat ttcagaacag ccgacattgt gagaggaggg caaggtgcac
     5401 ctctggtacc cattggcgat gaattgcttt ttaattccta ttccgcttgc atcaatctgg
     5461 gagggtttgc caatatttcc tataataaag atgggaaacg catagctttc gatttatgtc
     5521 cggccaatat ggctttgaac tttcttgctc aaaaagcgaa tctgcccttc gactataatg
     5581 gagaattggc cgcctctggt cgcatcattc agcctttgct cttcgaactt ctgcatctcg
     5641 agtatttctc gaaaaatccc cctaaatcac ttggaaagga gtggttcttg caatcttttc
     5701 ttcccatttt aaataagtac gaaggcgagc agattcaaga tctttcgcgt acggtttgtg
     5761 agttgattgc tattgtgatt atgcgttcat tatttgatgt ccaaccaaaa gaaaaaggcg
     5821 ttttaattac cggaggcgga gcgcatcatc attcccttct acagagaatt aaagaattat
     5881 ctactttacc tattgccatt ccttcccctt gtattatcga cttcaaggag gctttggttt
     5941 ttgcctttct cggattattg tttctcgagc aaaagccaaa tattttgtca tcggccacag
     6001 gagctgaaac tgatagcatt ggtggatgtc tttatttgcc atgatttatt atcgatttgc
     6061 tttgttttat aatcagttac tatttagaga attgaaatta catccctgaa ttttcgtccc
     6121 taatcctgat ttcatcgggg tattaaaata ttcaaataaa tatcttcgaa aatataataa
     6181 tgagcaatag ggaaaattta taattttgcc tggtgtaatt gaaaatgcaa tggatagcaa
     6241 actacaggaa ttgacggaaa gaatctatca cgagggtgtt gaaaaggccc gggcagatgc
     6301 ggctgctata ttgggaaagg ccaataaaga ggccgaagaa attttacaga atgcaagaca
     6361 tgaagccagt cgaattatag agcaggcgcg tcgtgaagcc gaagagctca agcgcaatgt
     6421 aaccaacgaa atcaaattat cttcgcgtca ggccatctcc tcactcaggc aacagattac
     6481 cgaacttata cttaccgaaa tggttgatca gtctgtcaaa gaggctttca acgataaaga
     6541 ttttatcaaa cgcatgatcg agactatcct tacgagatgg caacccgata attcgggtaa
     6601 agggcaaagc gttcagctct ttttacctcc cgaagatgaa gaaaaattag gaaaatattt
     6661 cgaagccaga gcaaaagaat tgatgaacaa aggcctgaag gtgatttatg atgaaaaaat
     6721 aggaagaggt tttcgtattg gcccagccga cggtagttat gtaattagct ttaccgaaga
     6781 agacttcgag aactttttca agaattatct ccgcccgcgc accaaccatc ttctttacgg
     6841 agggaaataa tgtcgggaag aaattattat tatctggtag caggcttgcc cgacctggtg
     6901 ctcgagcaat ccaaacttgc cctcgacttt gccgaattca aactcgaatt gcggcatcat
     6961 ctctcggctt tcgactataa actcgttgaa ttgattcttt tacccatcga taataaaaat
     7021 cttcttcatc tccttttgaa aaccaatgaa gaatttattc ctaacggaaa gtattcttta
     7081 gaaatactcg aagaagaaat gaagaatccc aagattctcc ccgattatct tcgaaacttt
     7141 attcttcatt ttaaagaaga gcagcctgtt tatcccgagc taagctggga agatcaattg
     7201 acggccatat atcttgagta tgctgctcaa accaagaata aattcgtgtc gaattggata
     7261 aaattcgaaa ggaatgttag aaatatcatg gcggcgctta ctgccagaaa atttaaactc
     7321 aatgctcgtt cgttcattat tggcaatgac gaattttcgc aaaccctcat taaaagtcag
     7381 gcaagagatt ttggacttgc acaacaattc ccttatatcg aaaaacttat tcagattttt
     7441 gaaaccaaca atttattaga aagggaaaaa tcgctcgatc ttttgcgctg gtcttatctt
     7501 gacgaattga atacttttca ttatttcagt atagagataa tccttgcatt tatattaaag
     7561 ttccaaatgg tacaaagatg gctgcggctc gacaaagaag caggcttagc catgtttaga
     7621 cgtttgctcg aggatttgga aaatagctat gaatttccta aagagttttc atgataatga
     7681 ataacacgaa aaaaactacc ggaaaggttt ccggaattat tgcgaatttg gtgattgtgc
     7741 aggttgaagg gcctgttgca caaaatgaga tttgttatat cagccatgat ggaaccgatc
     7801 ttatggcaga agtaataaaa attatcggta ataaggcata tactcaagtt ttcgaaagca
     7861 cccgtggatt gaaaatcggc tcaccagcaa tattcgaagg acacatgctc gaagccaccc
     7921 ttggccctgg aatcctttcg agtgtttatg atggattgat gcacgattta catgaaatga
     7981 cagggatttt tcttaaaccc ggtgaatata cacgtgcttt agatcctgaa aagatttggg
     8041 atttcaagcc tctggcaaaa ggcggagatc aggtgatagc tgccgactgg ctgggcgaag
     8101 tgatagaaaa ttccatccct catcgcatta tggtgccttt cgttttcgaa gggagatata
     8161 ccattaaatc cattgttcct cgtggtcttc ataagattta tgataccatt gccgtgctta
     8221 cggatgaaag tgggaaagac catcccgtta ccatggttca atattggcct gtaaaacagg
     8281 ctataaaggc atacaaggag aaaccccgac ctttcaaact tttccaaacc ggcgttcgta
     8341 ccatggatac tctgaatcct atggccgaag gaggtacggg ttttattccg ggaccctttg
     8401 gtagcggcaa gactgtattg caacacgcgc tgtcgcgtca agccgaagcc gacatagtta
     8461 tagtcgccgc ttgtggcgaa cgtgccaatg aagtggtcga gctcttcgta gaatttcccc
     8521 aattagaaga cccaagaacg ggacgtaaac tgatcgaaag gactaccatc attgccaata
     8581 cctctaacat gcccgttgca gcccgtgaag cctcggtgta tacggctatg accatcgccg
     8641 aatattaccg ttcgatggga ttgaagatat tacttctggc cgactctacc tctcgctggg
     8701 cacaagcctt acgcgaaatg tcaaatcgta tggaagaact ccccggcccc gatgccttcc
     8761 ctatggacct gcccgcaatt atctcaaact tttactctcg tgcagggtat gtctatttga
     8821 acaataacca aaccggaagc attacattta ttggaacggt ttcgcctgca ggcggaaatt
     8881 tgaaagaacc cgttaccgaa tcaacaaaaa aagtagctcg agctttttat gcactatctc
     8941 agcaaagagc tgatagcaaa cgttatcccg ccatcgatcc tatcgaaagt tattctaaat
     9001 atctcgaata tcccgaggtt caagaatcat tgcgcaatac cattgccgat gattgggtcg
     9061 ataaggtgat gctggtgaaa aatatcatga tccgtggacg tgaagcttcc gaacaaatca
     9121 atattctggg cgatgatagt gttcctctgg attatcacct tactttctgg aaatcagaag
     9181 taatagattc cattattctt caacaggatg ccttcgacaa gatcgacttc aatactccca
     9241 tgacgcgcca aaaatatatg ctcgacaagg tgataaatat ttacgaaacc aatttcgatt
     9301 tcgacagctt cgaagatgtc aacccctact ttaaacgaat tattaatcaa ttgaaacaaa
     9361 tgaattattc cgaatttcaa tcggaacagt ttaaacattt tgaaaatgaa ttatataaaa
     9421 tcattgagga aaggaagtta aaaaaatgag tccacttgca ttccagaaaa tatataccca
     9481 tatcagcaat attaccaagg ctacatgcac gctgaaagct tcgggagttg gcaacgaaga
     9541 actggcactg gtgcatggac gcctgtctca agtggtaaaa attatcgata atgaagtaac
     9601 acttcaggta tttggtggta cagaaggaat tccttccaat gccgaagtta ttttctataa
     9661 ccgtcctcct acactcaagg taagcgatga cttggcaggt cgttttttta atgcttatgg
     9721 tcagcctatc gatggaggtc ctgaggtaga gggtgaagaa cgtcccattg gtggaccttc
     9781 ggtaaacccc gttagaagac gccaaccttc ggagctcatt gccaccggaa tagctggtat
     9841 agatttaaat aatacgctgg tcaccggaca gaaaattcct tttttcgccg accccgatca
     9901 acccttcaat caggtaatgg ctatggtagc tctccgtgcc gaagccgaca agatcattct
     9961 gggcggaatg ggcttgacga acgacgacta cctttactac aaaaatgtgt tcgaaaacgc
    10021 cggtgccctc gaccgtatca tcagtttcgt aaatactacc gaagatccac ccgtcgaacg
    10081 cctactggtt cctgatatgg ctcttacggc agccgaatat tttgcagtcg ataaaaacga
    10141 aaaagtcttg gtgctgctta ccgatatgac actttatgcc gatgccctca gtatagtctc
    10201 caatcgtatg gatcagatac cttctaaaga ctccatgccc ggctcacttt atagcgattt
    10261 agccaagatt tatgaaaagg ctgtccaatt tcccagtgga ggttccatta cgattattgc
    10321 agttactacc ctttcaggag gcgatattac ccatgccata cccgataata cgggttatat
    10381 caccgaaggc cagcttttcc tgcggcgtga tacagatatt gggaaggtta tcgtcgatcc
    10441 tttccgcagc ctttcacgac tgaaacaact cgtcatcgga aagaagacac gtgaggatca
    10501 tcctcaagtg atgaatacag ccgtacgtct ttatgccgac gccgccaacg ccaaaactaa
    10561 actcgaaaat ggcttcgacc ttacggatta cgaccagcgt gcactcgatt ttgccagaga
    10621 atattccgaa tatcttctgg ctattgatgt aaacatcgac atcgataaaa tgctggatac
    10681 aggctgggaa ttgtttgcac gccattttaa ccaggccgaa gtgggcataa aacaagaact
    10741 cgtcgataaa tactggcctg taaaataaaa cacaatatgt aaagaatgga aaattttcca
    10801 atgataaagc tataaaagta atgagtagaa aatggcggta aaatttcaat ataataagac
    10861 ttctcttcag gagatgaata agcagctccg gatacgcctg cgagcattgc ctactttaaa
    10921 aaacaaggaa tcggcattaa gagcagaagt gaaacgagcc aaggattatg ccacaagcct
    10981 cgatgaaaag ttgtctgctg ctatggccga atacaatgaa atggtgcgtt tatggaatga
    11041 gttcaacccc ggattggttg tcattcatga tgttgacttg gggattaaga aaattgccgg
    11101 agtgcgtatc cctatccttc agcaaatcca tttcgaaata aaacccttca gcctcttcaa
    11161 tcaaccagtt tggtacatgg atggcatcct tattctcgag aaacttgcct ctattgctat
    11221 cgaaagagaa ttttactacg ccaagatgaa attgctcgat catgcccgta aaaaaactac
    11281 ccaaaaagta aatctttacg aaaaagttca gattccaggt tatgaagaag ctgttatcaa
    11341 aataaaacgc ttccttgaag acgaagaaaa cctttccaaa tcttcgcaga aaatcttgaa
    11401 gaatcgattt gaccaaaagg aggttgcggt atgatagtcc ccatgaccaa attcattttt
    11461 ttggtatatc atgaagatta tgaacgattt ctaaagggct tgcgatctgt gggtgttata
    11521 gatatcgtaa agcggaagaa ggaactcgac aaagacgcag ctgaaaggct tgccttgcaa
    11581 aagcaggttg ctgaaaccat caagttgctt aaaaagcata aaaaacccga aatcacagct
    11641 gaagcgccct cagaagggga tggaatgaaa atatttgaac acattagaga tttacaggca
    11701 gaggtcgagt ctttaaatca gcaactcgct acattgaaaa aggaaattga atatactgaa
    11761 ccctggggag atttcgaccc tcaaattatc gaaaaacttc gtaaagcagg attgagaata
    11821 tctttttata tcataccttc tcgtaaattc gatcctcagt ggttatcgaa atacaatatt
    11881 ggggtggtga atgaacagcc gggtgttatt tatttcattc ttgtgaatga aggtcaggaa
    11941 gagattgatt tgccggccga agaagttaaa gcacccgaac gtcctctgag tgaattgtta
    12001 aaaaaacagg aagctataca gacacgcctt agcgagattg aagaagaatt tgcaaggctg
    12061 gctgcgagct ctattcctct tttgcagaaa acattgcatc aaattgcctc cgaagtcgat
    12121 tatcatgtag tagtaaacaa cactttatcg gaagcagaag ggaagttgaa gattttagaa
    12181 ggttttgccc ctgttgaaac aaaagaaacc gttgaaaagt attgcgaaga aaatggtatt
    12241 ttttatatcg aaactcagcc agatccaaac gaaaaggttc ctatcctcct gcgcaacaat
    12301 agtttcgcga aattattcga gcccattggc cacttatatt ctttacccaa gtatggcgaa
    12361 ctggatctta ctccattctt tgcccctttc tttatgatgt tttttggctt ctgcttaggt
    12421 gatgctggtt acggattgtt tatatttatt gctgctacct tgtttaagaa attcaaagct
    12481 aaaccttcat tcaaacctta tgtaactctt gcacagtatc ttagtttagc tacctttatt
    12541 ttcggagcaa tttcgggaac cttcttcggt atcagtctta tagatgctga tattcccatt
    12601 gtggaaaata tcaaatccta tttcttcgat agtaacaaga tgctggtgct ctctttgata
    12661 cttggagcag tgcaaattgt attcggaatg tgtatcaaag ttgttaatat tactcatcaa
    12721 gaagggtttc gaaatgcact ttcattggtt ggctggttga tccttatttt gggactttta
    12781 atccgtcaag gattggtttc ggccggcgtt ctacctaaag gagaaaccat cgttctatat
    12841 agtattttag gagtttcctg cttattgatc tttttcctaa atgatttaaa aaccaatgtt
    12901 tttgtgagaa ttgggaaagg tttatgggat gtatatggaa tggcaacggg tatatttggc
    12961 gatttactct cttatattcg actttttgcc ttgggaattt ccagttccat acttggaata
    13021 gtaatcaata acgtggctat gtctattctt ggaattccct atatcggacc tcttcttttc
    13081 gtggtattcc ttgttattgc tcacttgggc aatattgcca ttagttctct tggcgctttt
    13141 gttcatccta tgcgtctaac ctttgttgaa ttttacaaga atgctggttt caaaggagga
    13201 ggaaaacaat ataaaccatt tgccgagaaa cgataattta tagtctatgc gacaaatcag
    13261 attcatcaat aaaccaatca tttaaaactt ttttcgatta tgacaactgc agtaatttta
    13321 gcttatgtag gacttgccat gatgcttgga ttagcaggta ttggcagtgc tattggggta
    13381 actatttgtg gtaatgcgac cataggggct ctgaaaaaaa atgaagaagc ctttggtcca
    13441 tatatgttac tgagtgcttt gcccggaaca caaggtttgt acggttttgg aggtttcttc
    13501 atcatcaatg caaaacttac accccaattt attgctagta tgactttagg ccaaggtatg
    13561 gccatttttg ctgctggcct tgtacttggg ggtgtaggct tgtattcggc tatacgtcag
    13621 ggacaagtat gcgccaatgg gattgctgga ataggttcgg gttacgatgt ttttggtaaa
    13681 acaatggttt tggccgtttt ccctgaactt tacgccatca tttcatttgc agctactttt
    13741 atcatcagcg cagctttgta gtatttttac caaagtatta tcgtatttcc tcccaactgg
    13801 ttatggatga cttctcctta atcagttttt cattcaccaa ttatggaaat aaaacctacc
    13861 gttgttcttg gggttagccg aaaccctgaa cgcatggcta atcaggcggt tgaagcttta
    13921 ttaaaccatg gacatcctgt ttatgccatt ggaaaagagg attttatttg gggtaaaatt
    13981 gtggttcatt caacctggga tcaagcagta gatatacata ctgtttctat ctaccttcga
    14041 ccggaattac aaaaggtaca tcagcaaagg attctatcag tgaatcctca aagaattatc
    14101 tttaacccag gaacatataa tcctgaatta gcaatagctg cttctgagag aggtatagaa
    14161 attttggaag aatgtacact tgtattgctt tcttcaggcc aattttgaac atctacaaaa
    14221 ttttttttaa tgtggctcgt tcattatatt ctacaatgaa ttttattaca atcaagtggt
    14281 aaaaatttct cagatgaaac gtattctcat tattctattt ctaacatgtt gtgctttacg
    14341 gcttcctgcc caaattttaa tttctttcaa aaccgatagt acttgtaaaa ccgacactat
    14401 tattgttcca ttaaaggttc aggaattaag gcaatgtgcc tcctttcgga ttgaattttc
    14461 atttcaggat gatattgttc attatgacag tctttatttt cgtaaccctg cactggccgg
    14521 tcttaccgct ttcttaaacg gaaataatcg tctggttctg gagtggacat cggcctccgc
    14581 tttacccttt ggaacgggca atattgcgaa gattcaattt acagccctgc aaagtggtac
    14641 ttctttactt tcttttgatg ctgctcaaac ctacttcaaa gatgagcaag gcaatcctct
    14701 aacttattta tataacgatg gtatcctgaa ggttcatcca cggggca
//
LOCUS       DAOORU010000043        14289 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_5195_length_14289_cov_5.180132, whole genome shotgun sequence.
ACCESSION   DAOORU010000043 DAOORU010000000
VERSION     DAOORU010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14289
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5195_length_14289_cov_5.180132"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="PLF20_04285"
     CDS             <1..1083
                     /locus_tag="PLF20_04285"
                     /inference="COORDINATES: protein motif:HMM:NF021554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HOX80261.1"
                     /translation="TDWIFIRLCVNRKFSCKFGILSLNNSRVPKPFIAIFTVTLVYFP
                     VFLFPLALEESLYDSQDSIIAADTLKTLRWWESAIEYSPQRMRYIVGGGSLMYGVTLV
                     GLNYLWYEDYPRSSFHFFDDSGEWLQVDKAGHVVTPYLEAYYLQYIFRWAGMKPKPAA
                     IYAGITAFMLQNTIEVFDGFSAKWGASWSDVAANFVGSALMTSQELLWNEQRMRLKII
                     HHFPDYPQGELSQRARDLYGKTLPERLFKDYNALTVWLSMNPSSFGLSLGQPEWLNLA
                     MGYGAANMYGGYNNIWTDENGILHDRSDIERYRRYFISPDIDFSRIKTKSRFLRIFFE
                     TLNIIKVPAPTLEINSSGQIIFHLLY"
     gene            1181..1822
                     /locus_tag="PLF20_04290"
     CDS             1181..1822
                     /locus_tag="PLF20_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80262.1"
                     /translation="MLGDVLLITDKHREAAASILNKVLEIRKSKMIIAISGESGSGKS
                     EISHVLAKALRTNGILTKVMHSDNYYRIHPLERKEWRIQHGIETVVGYNEYDWNNIYR
                     NIDEFRQGKVANMPCVDLVTEQVDQLITDFENIDALIIDGLYAIKTEGTDLNIFIELT
                     YHETKKAQMVRGKEPQNEYRARVLEQEHKMVQALKPLSHLLITPEYKVVPARG"
     gene            complement(1934..2146)
                     /locus_tag="PLF20_04295"
     CDS             complement(1934..2146)
                     /locus_tag="PLF20_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOX80263.1"
                     /translation="MKTLRFKTNLHCQHCIQKITPLLNAEEDIASWHVDLETSDKVLT
                     VKGDINPEKIISELEKIGYRAQLINE"
     gene            complement(2143..4353)
                     /locus_tag="PLF20_04300"
     CDS             complement(2143..4353)
                     /locus_tag="PLF20_04300"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOX80264.1"
                     /translation="MAFQFITISVKGMTCASCVATIEKQIKKIEGVQEVSANLALENV
                     SISYDPDKTSPAEFVEKILKAGYQTPLSSSQSANSSSVTFEEMNVSKLRQVWIAALPI
                     LIFGIFFNNFRPGQFLSFFITVYLMVFPARYIYKKAWRQLKSLTPAMESLVAISTLAA
                     LIFSAVILWIPAMHGGHLWFETPAMIIAFVMTGKFLEERAKRQSAASLQKLKEIQPNK
                     ARVIRNGQEIEIEVASAMTNDRVLVKAYEMIPVDGKILIGSSHIDESSITGESMPRFK
                     TKGDEVYAGTRCLEGALTLLVTRSPSETVLANVIAAVTRALSTKAPAQRLADRIAGVF
                     VPVALLISLISFAAWMFFQKEGQFAMALTAMVNVLIIACPCALGLATPTALVAAIGKA
                     ALNGILFKDGKTIEKTATTSLVAFDKTGTLTLGRPVIKDHYLDSSNHPDLLQWIAVFC
                     AMEKASHHPLSNYLVSYLQNIFPSFNLPFIEQQVEVIPGKGVIYKNHGQIFRLGSLQW
                     MRSEGTDISQDMQIILEEWQNHAFALVIFAIEHKAIMALAAGDALRTDAADCVKLLKK
                     QGIRSIMLTGDHKTSAKIIAEQVGIPTFYAELLPDDKRKIIDDYMKKGETVLMIGDGI
                     NDAEAMASAQISATLGNSTSLALDLAGITLTHRKLLHLPAAIQLSRTTRRIIRQNLMW
                     AFLYNVLCIPIAAGLLYPFTGELLNPMIAAAAMSVSSVLVVSNSLRIKKIKLAI"
     gene            complement(4356..7196)
                     /gene="uvrA"
                     /locus_tag="PLF20_04305"
     CDS             complement(4356..7196)
                     /gene="uvrA"
                     /locus_tag="PLF20_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOX80265.1"
                     /translation="MIPASHIQDLDYHQYILIKGARQHNLKNFDLAIPKDKLVVITGL
                     SGSGKSSLAFDTLFAEGQRRYIESLSAYARQFIGRMSKPDVDFIHGIAPAIAIEQKAN
                     TRNPRSTVGTSSEIYEYLKLLFARIGKTYSPISGNIVKRNTPTDVTDFIAKLPQGSRS
                     ILLSPIHQNSGENLSSRLNIFLQQGFTRVSINGNIFHIEEILSSQNIIDSNAEIQLVI
                     DRFTGGDIDEEFVSRIADSVEIAFYEGMGTCMVDYWQNGELHRQIFSNRFEADGIAFE
                     EPSVNLFTFNNPYGACKTCEGFGSVIGIDEDLVIPDKSLSVYEECVAPWKGEKMGEWR
                     WQFIQAARSRGFPIHKPYDELNEEQKQLLWKGDRGILGINDFFEMVQENLYKIQYRVL
                     LSRFRGKTVCPDCKGTRLRKEATYVQVGGYAITDLILKPIKQLQEVFEQLTLSDSDFL
                     AARRLIIEIDARLKVLNDVGLGYLTLNRNSNTLSGGESQRLNLATAIGSNLTGSMYIL
                     DEPSIGLHARDTQRLIQVLCNLRNIGNTVIVVEHDEEIIKASDQIIDIGPEAGVYGGE
                     LMFQGSYQEMIKYGNSLTAKYLSGQMGIPIPKHRRHWNNYIEIIGASENNLKNIDVKF
                     PLNVLTVITGVSGSGKTTLVKQILYPALRKLYQGYAEKTGRYLKLSGDYKMIRNTEQV
                     DQNPLGRSSRSNAATYSGAYDDIRTLFSTQPLSRVRGYKPGYFSFNIEGGRCEECQGE
                     GRIKIEMQFMADLWLVCDSCKGQRFKEEVLEIKVNGLNINEVLNLSIDEAVRFFQHPS
                     ISSDIARKIIAKLKPLMDVGLGYLHLGQSTGTLSGGEAQRLKLASFISNIQNERPTLF
                     IFDEPTTGLHFHDISRLLAAFEALLNNGHTLIVVEHNPEIIKSADWVIDLGPEGGDEG
                     GYLVFEGTPEDLIKHPTSYTAQFLRKKLEEKN"
     gene            7511..8095
                     /locus_tag="PLF20_04310"
     CDS             7511..8095
                     /locus_tag="PLF20_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX80266.1"
                     /translation="MNYTALSDQELIKNYRSGDSDSLEHLILRHQEKVTSYVLNVVKN
                     RHLADDIVQDTFLKVINTIRSGAYKEEGKFIQWVMRIAHNLMIDYFRRQKKFPIAEPG
                     EGMDIFSTLPLQDRSVEDAIIYEQILTDVKRMLKYLPEEQRRVVEMRLYEDLSFKEIA
                     EITKVSINTALGRMRYALINLRKIAKENDIQLSM"
     gene            8558..8722
                     /locus_tag="PLF20_04315"
     CDS             8558..8722
                     /locus_tag="PLF20_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80267.1"
                     /translation="MDKNSTHEIQQKFLFKRKHFVPMPGGRLAFLMAYSHALLVVSTR
                     MIGDCFINMN"
     gene            8898..9461
                     /locus_tag="PLF20_04320"
     CDS             8898..9461
                     /locus_tag="PLF20_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80268.1"
                     /translation="MILFFYVIQLNAQKNNELGIHFGLMANSLVSNANLDGSGSFDGK
                     GAFVLGVKYVRNISNHFSFETGIGYSENKIKIIPAPGLGLVPSEKTIQLVSLPILGNF
                     SFSRNFYLNGGILMDLEIYHSNIQSTHKQSGIGVYLGVGGQYNIERFTLKLIPFVQRH
                     AIIPFEHEKYQQHLTEVGITLVLGYQF"
     gene            complement(9509..10465)
                     /locus_tag="PLF20_04325"
     CDS             complement(9509..10465)
                     /locus_tag="PLF20_04325"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HOX80269.1"
                     /translation="MKKFGLLIFVILIGISFKAVSEDNADSTRSWKLNGNSTLTFNQL
                     HFSNWAEGGESSLSGSGWLNVKVGHYWKKLNSERNLILGLGVLRTQERDLRKTEDKID
                     YTSIFGYKAFKNWNYTSLINFKSQFTNGYRYPDDSTLISTFMAPGYLTLSLGMQYKPS
                     DNFTIFISPASGRFTFVLNEQLANKGSFGVKPAVYSNDSLHTLLTTGKKIKPEFGINV
                     NAQLKREIFTNVSIESRLILYNNYLDEDIHNRWNIDVNWDALINFHINKFLSSSLRLN
                     LLYDHNIKIKQYAEVDGHQVVIGEGPRLQFKESFGIGLNYKF"
     gene            complement(10740..11156)
                     /locus_tag="PLF20_04330"
     CDS             complement(10740..11156)
                     /locus_tag="PLF20_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOX80270.1"
                     /translation="MSDKVSLNWMEDLAFEANVNGHKIIMDAVESAGGRNRGPRPKPL
                     VLAALAGCTAMDVIPILRKMQVIPEGFGIEVEAETTEEHPKVYHTIKLIYIFKGQNLP
                     IDKLQKAIDLSQERCCGVSAMLRKAAHISYEMRIEN"
     gene            complement(11183..12238)
                     /locus_tag="PLF20_04335"
     CDS             complement(11183..12238)
                     /locus_tag="PLF20_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80271.1"
                     /translation="MLYKKFFYFFCFSLVMFISACSNEPENPENTSENNFSGRRGFFI
                     INEGLFQQGNASIDFYDLDTKEVIHDVFFTANQRPLGDVAQSLNKVGNKIWIVVNNSG
                     KIEIVKSKNFESIMTLTGFASPRYLLFHKNGKAYLSDLYKESLYVIDTLTGQTIKEIS
                     TGKSCEYLIEAGSHIFALNWSSLNDSLNNTLTIIDGLADSVVKSVILTKEPNSAAIDK
                     NGHIWILCSGGFFFEETPALYCMNPQGEILKKFEFSGNQTYPTRLCINPSHDTLFYLN
                     KDVYRMGIMENDLPQLPFIQSNNKNFYGLNAGNFILLSDPLDYQQNGNVYVYSRKGQL
                     LSTFQAGMVPSGFQWNE"
     gene            complement(12251..14182)
                     /locus_tag="PLF20_04340"
     CDS             complement(12251..14182)
                     /locus_tag="PLF20_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX80272.1"
                     /translation="MSTGLCQNSSPNFSAYSDSSHIYPLIHSNLYPDTIKLKSITVSA
                     KYPDVSYFSAYRIPKIEMEVHEERTLGEILQRVAPIYLKDYGPGNTSTITLRGTNAAQ
                     TQIYWNGIPINAAGTGQTDFSTIPVMSEDEILLLPGLVSLEKGSGGLGGSIEINNNPS
                     WNDNNRYEAKIETGSFGYQSYGGKAAIKHKNLQTKFYTRYQSADNDFEYINNMLAEPK
                     KEKRLGASNHQWLVGEDFYLNADSLNAIEIHLFTQSLNRDIPSPISTVQLPENEQMKL
                     DDARIMASWRHQSKNQIRLGLKSAWTYNFMNYQNKLAAINSTYTMHQGFLIINASKYW
                     ENNTHLESEIRFQSQMARTANYADNITRNISSLWIKLNHHLGSFANLYAGMRKEIAES
                     TSTPLLPVIGTELFPMATKNLAFYLAYAKNSRIPALNDLYWVPGGNPNLKPEISHHLE
                     SGLRFRQNLLNRISIGLQANAYSSRIKDWIAWMPDSVMISIWEPINLKHVKASGIEIL
                     IDIGCTWSKTSLNSRLSYAFNITNENEDDPQGRLQLPYTPQHIGNFSFYLVHRSFSFM
                     YSMNYTGKRFTNYDESHFLKPYLLQDTGLDYIFSIKKLEIKLTLKINNLFNIQWENIA
                     YQPMPGRYFHLGFTFLLNP"
BASE COUNT         4168 a         2704 c         2898 g         4519 t
ORIGIN      
        1 acggactgga tttttattcg tctgtgtgtc aatcgaaagt tttcatgtaa atttggcata
       61 ttatctctaa ataacagcag agtgccaaag ccattcattg ctatattcac cgtaaccttg
      121 gtctattttc ctgttttttt attcccactc gccttggaag aatctttata tgattctcag
      181 gattccatta tcgcagccga cacgcttaaa acattgcgat ggtgggagtc agctatcgaa
      241 tatagccctc aacggatgag atatattgtt ggaggagggt ctttgatgta tggggtgacg
      301 cttgtcggtt taaattattt atggtatgag gattatcctc ggtcatcgtt tcattttttc
      361 gatgattctg gggaatggct tcaggtagat aaggcgggtc atgtggttac accttactta
      421 gaggcatatt atcttcagta tattttccgc tgggcgggaa tgaaaccgaa gcctgctgca
      481 atatatgcag gaattaccgc atttatgctt cagaatacca ttgaagtatt cgatggtttt
      541 tcggcaaaat ggggggcatc gtggagcgat gtcgcagcaa attttgtggg ctcagctttg
      601 atgacttcac aggaattgtt atggaatgag caacgcatgc ggctcaaaat catccatcat
      661 tttcccgatt acccacaggg tgaactctct caaagagccc gtgatcttta tggaaaaaca
      721 cttccagaac gcttgttcaa agattataat gcattgaccg tttggctgag catgaatccc
      781 tcttcgtttg ggctatcctt agggcaacct gaatggctga atttagccat gggctatgga
      841 gctgccaata tgtatggcgg ttataacaat atatggacgg atgagaatgg aatattgcac
      901 gatagaagcg atatagagcg ctaccgaagg tattttattt ctcccgatat cgatttcagc
      961 cgaataaaaa ccaaatcaag gtttttaagg atttttttcg aaaccttgaa tatcatcaaa
     1021 gttcctgcac caactctcga aatcaattca agtggacaga taatttttca tcttctatat
     1081 taaagtaata ttgatggaat ttctctgtaa cttgtttcca tttcgataat tgtgcaatag
     1141 ggacaagttc tatatttttg tttcgtaaaa ttttgaaaat atgttgggag atgtattatt
     1201 gatcaccgat aaacaccgtg aagctgcagc aagtatattg aacaaagtcc tcgagattcg
     1261 taagtccaag atgataattg ccattagtgg agaatcggga agtggaaaat cggagatttc
     1321 gcatgttctg gcaaaagcac tcaggacgaa tggcattctt accaaggtaa tgcatagcga
     1381 caactattat cgtattcatc ccttagaacg taaggaatgg agaatacaac acggaataga
     1441 aaccgttgtg ggatataacg aatacgactg gaacaatatt taccgaaata tcgatgaatt
     1501 ccgacaaggt aaggttgcta atatgccatg cgttgattta gttaccgaac aggtcgatca
     1561 attgattaca gatttcgaaa atattgatgc gctgattatt gacgggcttt atgcaataaa
     1621 gacagaagga accgacttga atatttttat cgaacttacc tatcacgaaa cgaaaaaggc
     1681 ccaaatggtg agaggcaaag agcctcagaa cgaatatcgt gcgcgtgtac tggaacaaga
     1741 gcataaaatg gtacaagcac tgaaacccct atctcattta ttaattactc ctgagtataa
     1801 agttgttccc gccagaggtt aaagagttca ttttggcctc gaatctgcat tgtcagtatt
     1861 tccaaaacca attctggtaa atactttaac cgtacattct atacgatctc gaatccttta
     1921 ttagaaacaa aggtcattca tttatgagct gggctctata tcctatcttc tccaattcac
     1981 tgatgatttt ttcggggttg atatcacctt ttacagtcaa gaccttatcg gaggtttcga
     2041 gatcgacatg ccatgaagct atatcttctt cggcattcaa aagaggtgta atcttttgta
     2101 tgcaatgctg acaatgaaga ttggttttaa atcttagcgt cttcatattg caagttttat
     2161 ttttttgatt cttaaactat tggatacaac cagcactgag ctcaccgaca tcgctgcagc
     2221 ggcaatcata gggttaagga gctcaccggt gaagggatat agcaatcctg cggctatggg
     2281 gatacataaa acgttataga ggaatgccca cattaaattc tggcggatta ttctccgcgt
     2341 tgttcgagag agttgaatgg cagcaggtag gtgaaggagt tttctatggg taagtgtaat
     2401 tcctgcaagg tcgagggcga gtgaggtact attccctaac gttgcactga tttgggcact
     2461 agccatggct tctgcatcat tgatgccatc accaatcatc agcacggttt cacccttttt
     2521 catataatcg tcgataattt ttcgtttatc atcgggtaga agttcagcat agaaggttgg
     2581 tataccgact tgctcggcaa tgattttagc tgaagtttta tggtcgccgg taagcataat
     2641 agatcgtatt ccttgttttt tcaataattt cacacaatct gctgcgtcgg ttctgagagc
     2701 atcaccggct gcaagagcca tgatagcttt atgttcgata gcaaagatta cgagggcaaa
     2761 agcgtgattt tgccattctt cgagtattat ttgcatatct tgggaaatat ctgttccttc
     2821 gctccgcatc cattgcaaac tgcctaatct gaatatctgg ccgtgattct tgtagataac
     2881 ccctttccca gggatcacct cgacttgttg ttcgataaaa ggcaggttga aagaaggaaa
     2941 tatattttga agataggaga ctaaataatt ggaaagggga tgatggctag ctttttccat
     3001 agcacaaaaa actgcaatcc attgtaaaag gtcaggatga ttcgacgaat cgagataatg
     3061 atcttttatt acgggtctcc ccaatgtaag cgttccggtt ttatcgaaag ctacgaggct
     3121 tgtagtagcg gttttttcga tagtttttcc gtctttgaag agaatcccat tgagagcagc
     3181 tttgccaatg gcggctacga gagcagttgg cgtggccaat cccagagcac atggacaggc
     3241 aattatcaac acatttacca tagccgtgag ggccatggcg aattgtccct ctttttgaaa
     3301 aaacatccaa gcagcaaaac ttatcaaaga aatcaaaagg gcaacgggca cgaaaactcc
     3361 tgcaatacgg tcggcaaggc gttgagcggg tgcttttgtc gagagcgcac gtgttacggc
     3421 tgcaataaca ttcgctaaaa cagtctcaga agggctacga gtaaccaaca acgtcaatgc
     3481 tccttccagg catcttgttc cggcataaac ctcatctcct ttggtcttga atcttggcat
     3541 tgactcgccg gtaatcgaac tttcatctat gtgtgagctt cctatgagaa ttttcccgtc
     3601 gacaggaatc atttcgtagg ccttcactaa aactctatca tttgtcatcg cactggctac
     3661 ctctatttct atttcctgac cgtttcgaat tactctcgcc ttattgggtt gaatttcttt
     3721 gagtttctga agtgaagctg cactttgtcg tttagccctt tcttcgagaa attttcctgt
     3781 cattacgaaa gctataatca tagctggagt ttcgaaccaa agatggccac catgcatcgc
     3841 aggaatccat agaataactg ctgagaagat gagtgcggct aaagtgctta tggctaccag
     3901 agattccata gccggagtaa gagatttcag ttgtctccat gcctttttgt aaatatatcg
     3961 ggcagggaaa accatcagat aaacggtaat aaagaaggag agaaattgtc caggacgaaa
     4021 attattgaaa aatattccga atattaggat aggtagggct gcaatccata cctggcgtaa
     4081 tttactcaca ttcatttctt cgaaagttac tgaggatgaa tttgcactct ggctcgaaga
     4141 gagaggagtt tgatatccag ccttcaggat tttttcaaca aactcggcag ggctggtttt
     4201 atcggggtcg tagctaatgc tcacattttc gagtgccaga ttggcgctca cttcttgtac
     4261 tccctcaatt ttcttgattt gtttttcgat ggtggctaca catgatgcac acgtcattcc
     4321 cttgacagat atcgtaataa attgaaaggc catttttagt ttttttcttc taattttttc
     4381 ctaaggaatt gagcagtata agaagtagga tgttttatca agtcttcggg tgttccctca
     4441 aagacaagat atccaccctc atcgcctcct tcagggccaa gatcgatgac ccaatctgcc
     4501 gatttgataa tttccggatt atgttcgaca acaattaatg tatggccatt gttcagcaaa
     4561 gcctcgaaag cagccagtaa ccggctgatg tcgtgaaaat gtaatccggt agttggttca
     4621 tcgaaaataa aaagtgttgg cctttcattt tgaatgtttg aaataaaaga agccaatttc
     4681 aatcgctgtg cctcacctcc cgatagtgtg cctgtcgatt gtccaagatg gagataaccc
     4741 aggcccacat ccatcagggg tttgagtttt gctatgattt ttcttgcaat atcggaggag
     4801 atggatggat gttggaaaaa tcgaactgct tcgtcaatgc tcaaattcaa aacttcattg
     4861 atattaaggc cattcacctt aatctcgagc acttcctcct tgaatctttg tcctttacaa
     4921 ctatcgcaaa ccagccaaag gtcagccata aattgcattt cgattttaat tctgccttcg
     4981 ccttggcatt cttcgcagcg cccgccttct atattgaagc tgaaatagcc aggtttgtag
     5041 cctctaactc ttgacagagg ctgtgtacta aagagggttc gaatatcatc gtaggcgccc
     5101 gaataggtgg ctgcgttcga acgtgaagat cttcccaatg gattttgatc aacctgttca
     5161 gtatttcgaa tcattttata atcgcccgat aatttgagat atctgccggt tttttctgca
     5221 tatccctgat agagcttccg cagggcgggg taaagaattt gttttaccag agtagtcttg
     5281 cccgaaccac ttacccctgt aattaccgtc aatacattta aggggaattt tacgtcgatg
     5341 ttttttaagt tattttcact tgctccaatg atctcgatat agttattcca atggcgtctg
     5401 tgtttcggaa tggggattcc catttgcccg ctcagatatt ttgctgtgag cgaatttcca
     5461 tattttatca tttcctgata cgagccctga aacatcaact ctcctccata aactccagct
     5521 tcgggcccaa tgtcgatgat ttggtcggaa gccttgatga tttcttcgtc gtgttctacc
     5581 acgatgacgg tattgccaat gtttcgaaga ttgcagagta cctgaatgag acgttgtgta
     5641 tcacgtgcat gaaggccgat gctgggttcg tcgagaatat acatagaacc agtaagattc
     5701 gaacctatgg ccgtagccag attcaatcgc tgagattctc cgccgcttag ggtattggaa
     5761 ttacgattga gggtaagata tccaagtcct acatcgttta aaactttcaa gcgggcatca
     5821 atttcgataa ttaaccttct ggcagcaaga aaatcggaat cgctcagtgt gagctgttcg
     5881 aaaacttcct gcaattgctt gatcggcttc aagatcaaat cggtaatagc ataacctccc
     5941 acttgtacat aagtggcctc ttttcttaat cttgtgccct tgcaatcggg acaaacggtt
     6001 tttcctctaa aacgagaaag gagaacacgg tattgaatct tgtaaagatt ttcctgtacc
     6061 atttcgaaga aatcatttat gcctaatatt cccctatcgc ctttccagag aagttgtttt
     6121 tgttcttcgt tcaattcatc atagggctta tgaatgggga aaccacgtga acgcgctgcc
     6181 tggatgaact gccaacgcca ttctcccatt ttttctccct tccatggagc cacacattct
     6241 tcataaaccg ataaactctt gtcggggatg actagatctt catcgatacc tatgactgaa
     6301 ccgaagccct cgcaggtttt acatgcacca taaggattgt tgaatgtgaa tagattgacg
     6361 cttggctctt cgaaggcaat tccatcggct tcaaaacgat tggaaaagat ttgcctgtga
     6421 agttcgccgt tttgccaata atcgaccatg caagtaccca tgccttcgta aaatgctatt
     6481 tctactgaat cggctatcct cgaaacgaat tcttcgtcga tgtctcctcc tgtaaaacga
     6541 tcaataacca attgtatttc ggcgttggaa tcgatgatat tctgcgaaga aaggatttct
     6601 tcgatgtgga aaatatttcc gtttatcgag actcgggtaa aaccttgctg gagaaagata
     6661 ttcaagcgtg aagagaggtt ttcgccagaa ttctgatgga ttggcgaaag tagtatgctg
     6721 cgtgaacctt gaggtagttt ggcaataaag tcggtaacat cggtgggagt attacgttta
     6781 acgatgttcc ctgaaatggg tgaataagtt ttcccaatgc gagcgaaaag gagcttcaaa
     6841 tattcgtaga tttctgagga ggtacctaca gtactgcgtg gattacgggt gtttgccttt
     6901 tgttcgatgg caatggctgg cgcaattcca tgaataaaat ctacatcggg cttgctcatc
     6961 cgaccaatga actgacgtgc ataggcgcta aggctttcta tatagcgtcg ctggccttca
     7021 gcaaaaaggg tatcgaaagc cagagatgat ttacccgagc ctgaaagccc cgtaatcact
     7081 actaatttat cctttggaat ggctaaatcg aagtttttca gattatgctg ccttgctcct
     7141 tttattaata tatattgatg ataatcaagg tcttgaatat gggaagcagg tatcatccat
     7201 gtaacaaatt aaatgtgcaa aattaatact aattcatagg ggatgctcct tagaaccctc
     7261 tttccaaaaa agatgaaagg attataattt attttattac tggtaattaa cccccttttt
     7321 gaggtaaaga aactataaaa gcagtaaaaa tttgaaaaaa gattgctttt tttaaaaaat
     7381 gatataaatt tgcaccattg attgaaagtg tcataaagaa agaatttgat tgttgaatta
     7441 aaaacggagg ctgcctatcg ctgaacttct acacttattg aaaccacgaa ccctttagaa
     7501 gcgcagcgtt atgaattaca cagcactttc cgaccaggaa cttatcaaga actaccggtc
     7561 gggcgattcg gatagccttg aacatttaat cctcagacat caggaaaagg ttacttcata
     7621 tgtgttaaat gttgttaaga atcgtcatct tgccgatgat atcgtacagg atacctttct
     7681 gaaagttatc aataccattc ggtcgggtgc ttacaaggag gaaggcaagt ttattcaatg
     7741 ggtgatgcgc attgcacata atctgatgat cgattatttc cgccgtcaga aaaaattccc
     7801 catagccgaa cctggagagg gaatggatat tttcagtacc ttgcctttgc aggaccgatc
     7861 ggtggaagat gctatcatct atgagcaaat ccttaccgat gtaaaaagaa tgctgaaata
     7921 tcttcccgaa gaacagcgca gagtagtaga gatgcgactt tacgaagatt taagcttcaa
     7981 agaaatcgcc gaaataacaa aagtaagtat taatactgcc ctgggacgaa tgcgttatgc
     8041 cttgatcaat cttcgtaaga ttgccaaaga aaatgacatt caactcagca tgtgaggaat
     8101 ccacattgct tttaaatctc atatcatcca gcgtttataa ataaagggga aagagaatcc
     8161 tgttgggtta aactttaatc tgtaatgctc tatattagga gcagtgaggt tgttggatta
     8221 gtttatgaac tattttgggg gtttcatcca tctttcccct tgtatagcct catcttgaac
     8281 ttgaatatga ttagatacgg gaaggaaatc tttaaggtgg tttctccatt tgtctttttg
     8341 taagcattca catctctttc taaggtttta ttcaaaaata ggcttccctt caaaaatgag
     8401 atcatccgtt aaatccggga aaattatgaa tgcgatgctc atgatgatgt ttgacgtgcg
     8461 tatagttctt cgaataaatt ctttttaagg aagatatact aaagccatag gcttagcgtt
     8521 tgaatagata attcaaagtt tcaccaaaat gcaatgtatg gataaaaatt ctactcatga
     8581 aattcagcag aagtttctgt tcaagagaaa acactttgtg cctatgccgg gagggagact
     8641 agcatttttg atggcttatt cgcatgcttt gcttgtagtt tcaacacgta tgataggcga
     8701 ttgtttcatc aacatgaact aaaactttta gctaatccga aaggggggtt gcgggcgcca
     8761 gcgaagctgg cgaccgcaat gtaaaatgtt ttccttttta gaacgtggat tttttaaaac
     8821 ttgtttatca tctttcgttt gcttacaaat ttaatttgtc ttgattgatg agaaatttta
     8881 ggaaattctt ttttttcttg attttgtttt tttatgttat tcagcttaat gctcaaaaaa
     8941 acaatgaatt aggcattcat tttggtttaa tggccaacag tttggtatcg aatgcaaatt
     9001 tagatggaag tggtagtttc gatggtaaag gtgcatttgt gctcggggtt aaatatgtga
     9061 gaaatatttc gaatcacttt tcttttgaaa caggtattgg ctattctgaa aataaaatta
     9121 aaatcatacc tgcacctggt ttaggtcttg ttccatcaga gaagacaatt caattggttt
     9181 cattgccaat tttgggaaat ttcagctttt caagaaattt ttaccttaat ggtggaattt
     9241 taatggattt agaaatatat cattccaaca ttcaatctac acataaacag tctggaatag
     9301 gggtttacct gggagtaggt ggtcaatata atatagaaag atttacatta aagctgattc
     9361 catttgttca gcgacacgcc atcattccat tcgaacatga aaaatatcag caacatctca
     9421 ccgaagttgg aattacgctc gtccttggat atcaatttta atgaatggaa cgaaagaatt
     9481 ttttagctga atttctattc ggaaaatctt agaatttata attcaatcca atgccgaaac
     9541 tttctttgaa ttgcaggcgt ggtccttctc ctattactac ttggtgacca tccacttcgg
     9601 catattgttt tattttgata ttatgatcgt aaagcaagtt aagccttaaa cttgagctta
     9661 aaaatttatt gatatgaaaa ttaataagtg catcccaatt aacatcgata ttccatctgt
     9721 tatgaatgtc ctcatcgaga taattattat aaagaattag ccggctttcg atcgaaacat
     9781 tcgtaaaaat ttcacgtttg agttgggcgt ttacatttat gccaaattca ggtttgattt
     9841 ttttacctgt tgttaataaa gtatgaaggg aatcgttgga atagacagca ggttttactc
     9901 caaatgaacc tttattggcg agttgttcat tgagcacgaa agtaaatcgg ccagatgctg
     9961 gagaaataaa gatggtgaaa ttatcggatg gtttgtactg cattcccaaa gatagggtaa
    10021 gataaccagg tgccatgaaa gtagaaatca aagtcgaatc gtcgggataa cgatatccat
    10081 tggtaaactg gctcttgaaa tttattaaag aggtgtaatt ccaattcttg aatgctttgt
    10141 atccgaagat gctagtatag tctatcttat cttcggtttt tctgaggtct ctttcttgtg
    10201 tacgaagcac acccaatccc aatattaaat ttcgctccga atttaatttt ttccaataat
    10261 gtccaacttt tacattgagc catcccgaac cgctcaaact gctttcacct ccttcagccc
    10321 aattactgaa atgcaattga ttaaaggtga gtgtgctgtt gccatttaat ttccacgacc
    10381 ttgtactgtc ggcgttatct tctgacactg ccttaaaaga aatacctatc aagataacaa
    10441 aaattaacaa tccaaatttt ttcatttttt ttggttctac ctttgtattg ttcaggctac
    10501 cagttactga acgaaaaatt atcaaaaact caaacattaa ttcttcacaa atctttgcaa
    10561 attttatttt ttaaacataa ataaaaattt tattgactta ggttttttca aatgaataaa
    10621 taatttttat acaagataag gtaatcatga aataggaaat ttattgattc atatcctttc
    10681 ctgtaatgtt tattacgtct tagcaaaaca aagcaaagaa aaattgtttt ctttatggct
    10741 taattttcta tacgcatttc gtaggaaatg tgggcagctt ttctgagcat ggctgaaacg
    10801 ccacaacagc gttcttgcga aaggtcgatg gctttctgga gtttatcgat gggcaaattt
    10861 tgacctttga atatgtaaat caatttgatg gtatgatata cttttgggtg ttcttcagta
    10921 gtttctgcct ctacttcgat tccaaatcct tcaggaatta cctgcatttt gcgcaaaatt
    10981 ggaattacgt ccatggcggt acatcctgcc agagccgcca gaacaagggg tttagggcgg
    11041 ggtccacgat tacgtccccc tgcgctttct actgcatcca taatgatttt atgcccgttt
    11101 acattggctt cgaaggctaa gtcttccatc cagttcaaag agactttatc actcatagat
    11161 ctaaatttta ttttttgcaa agttattcat tccattgaaa acctgaagga accataccag
    11221 cttgaaaagt ggataaaagt tgtccctttc tcgaataaac gtaaacattg ccgttttgtt
    11281 gataatccaa aggatcggac agtaaaatga agtttcctgc attcaaaccg tagaaattct
    11341 tgttattaga ttgaatgaat ggtaattgcg gaagatcatt ctccataatt cccatacggt
    11401 acacatcttt gttcaaataa aataaagtat catggcttgg gttaatgcac aaacgagtag
    11461 gataggtctg attacccgaa aattcaaatt ttttcagaat ttcaccctga ggattcatac
    11521 aatatagtgc cggagtttct tcgaaaaaaa atcctccact gcataaaatc cagatgtggc
    11581 catttttatc gatggcagca ctattgggct ccttggtaag gataaccgat tttaccacac
    11641 tatcggccaa gccatctata atagtaagcg tattgttgag gctatcattc aatgatgacc
    11701 agttgagtgc aaaaatatga ctaccagctt cgatgagata ttcacaggat tttccggtgg
    11761 atatttcctt gattgtttgt cccgtcaagg tatcgattac ataaagagat tccttgtaga
    11821 gatcactgag ataggccttg ccatttttgt gaaataaaag ataacgaggt gatgcgaaac
    11881 cagttagtgt cattatcgat tcgaaatttt tcgactttac gatttcgatt ttgccggaat
    11941 tattcaccac aatccatatt ttatttccca ctttgttcag gctttgtgcc acatcaccca
    12001 atggtcgctg attggcagtg aaaaatacgt catgaataac ttcttttgtg tccaaatcat
    12061 aaaaatcgat acttgcattt ccttgctgga aaagcccttc attaatgata aaaaagcctc
    12121 tacgtccgga aaaattattt tcggaagtat tttctggatt ctcgggctca ttgctgcatg
    12181 ctgaaatgaa catgacaagg gaaaaacaaa aaaagtaaaa gaattttttg taaagcatgg
    12241 tttttaattt ttaagggttt aataaaaaag taaatccaag atgaaaatat cggcctggca
    12301 tgggttgata ggcaatattc tcccattgga tattaaataa attattaatt tttaatgtta
    12361 gtttaatttc aagttttttt atggaaaata tataatctaa gccagtatct tgaagcagat
    12421 atggtttcag aaaatggctc tcatcgtaat tcgtaaaacg tttacctgtg taattcatcg
    12481 aatacataaa gctgaaactt cgatgaacga gatagaaaga aaagtttcca atatgttgtg
    12541 gtgtatatgg taattgaagt ctcccctgag ggtcatcttc gttttcgttg gtaatattga
    12601 aagcatagga aagtctgcta ttcaacgagg ttttgctcca tgtacagcct atgtctatta
    12661 aaatttcgat accgctggct tttacatgtt tcaaattgat gggttcccaa attgaaatca
    12721 ttacactatc gggcatccat gctatccaat cttttattcg ggacgaatag gcatttgcct
    12781 gtaaaccgat agaaattcga ttgagtaaat tttgtcggaa acgcaaaccc gattcgagat
    12841 gatggctgat ttcgggtttt aaattaggat tgcctcccgg aacccaatac aaatcattca
    12901 aggctggaat tctcgaattt ttagcataag cgaggtaaaa ggcaagattt ttcgtagcca
    12961 tagggaataa ttctgtccca ataacaggca gtaatggcgt cgaagttgat tctgcgattt
    13021 ctttcctcat acctgcataa agattagcaa aagagccaag atgatgattt agctttatcc
    13081 atagagaaga gatatttcga gtgatattat cggcatagtt tgcagtacga gccatttgcg
    13141 actgaaaccg gatttctgat tcgaggtgtg tgttgttttc ccaatatttg gatgcattta
    13201 ttattaaaaa tccctgatgc atagtataag tagaattgat tgcggctaat ttgttctggt
    13261 aattcataaa attataagtc caggccgatt tcaagccaag acgaatttga ttcttgctct
    13321 gatgacgcca ggatgccata attcgggcat catccaattt catctgttca ttttcaggta
    13381 attggaccgt cgaaatgggc gagggaatat ctctatttaa tgattgtgta aaaaggtgaa
    13441 tttcaatggc attcaaacta tctgcattta aatagaaatc ttctccaacc agccattgat
    13501 gattacttgc cccaagtctt ttctcttttt tgggttctgc cagcatattg ttaatgtatt
    13561 cgaaatcatt atcagccgac tgatacctcg tataaaactt cgtctgaagg tttttgtgct
    13621 ttattgctgc cttgccccca tagctttgat aaccaaaact ccctgtttct attttagctt
    13681 cataacggtt attgtcgttc cagctgggat tattgttgat ttctatgctt ccacccagtc
    13741 cgccacttcc tttttctaaa gaaaccaagc ctggtaaaag caaaatttcg tcttcactca
    13801 tcaccgggat tgtagaaaaa tcggtttggc cggttccggc cgcattgatg ggaatcccat
    13861 tccagtaaat ctgcgtttga gctgcattgg taccacgtag tgtgatagtt gatgtattac
    13921 caggtccata atctttcaaa taaatagggg caactcgttg aagtatttca cccaaagttc
    13981 tttcctcgtg gacttccatt tcgatcttag gaatcctgta agccgagaaa taactgacat
    14041 cagggtattt ggccgaaacc gtgatgcttt tcagtttgat ggtatctggg tacaggttac
    14101 tgtgaatgag cgggtaaata tgtgaagaat cactatatgc agaaaaattt ggagagctat
    14161 tttgacaaag ccctgtactc atgatgaata tcatcgggaa aataataaga ccatatcgaa
    14221 attgaacagg aatgatataa aatattaaag ttcaaaagtg aatagtttct tcaaactcat
    14281 aagtaggat
//
LOCUS       DAOORU010000044        14150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_5263_length_14150_cov_5.857396, whole genome shotgun sequence.
ACCESSION   DAOORU010000044 DAOORU010000000
VERSION     DAOORU010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14150
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5263_length_14150_cov_5.857396"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1432)
                     /locus_tag="PLF20_04345"
     CDS             complement(<1..1432)
                     /locus_tag="PLF20_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOX80273.1"
                     /translation="MISLNHISIHFTGENLLDDISFIIADHDRIGLVGKNGAGKSTLL
                     KIIKREISPDNGQIIYSQNTSIGHLPQDMTIESERTVIEEAQTAFDHILKLKVEIDQL
                     TDQLTVCTDYESESYSRLIQRFEESNHLFNLLGGNAMKGETERVLKGLGFLEEDFQRP
                     VNTFSSGWQMRVELAKILLRNPSVILLDEPTNHLDIEAITWLEEWIQNFQGALVVVSH
                     DRAFLDNVTHRTIEISQGKIYDFKSSYSEYIVIREEQLEQQLAAYENQQRQIAQIERF
                     IERFRYKATKARQVQSRIKLLEKMDKIEIDGLDRSSIHFRFPPPPSSGKIVVEIKNLS
                     KKYDNKVVLQNIDFTLVKGEKVAFVGRNGEGKSTLSKIIAAGLDYEGIFRLGYNVKIG
                     YYAQNQSELLDLNKTVYETLDETAKGEIRTRLKSILGSFLFSGEAIEKKVEVLSGGEK
                     ARLALAKLLLEPVNLLVLDEPTNHLDM"
     gene            complement(1523..1596)
                     /locus_tag="PLF20_04350"
     tRNA            complement(1523..1596)
                     /locus_tag="PLF20_04350"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1560..1562),aa:Arg,seq:ccg)
     gene            complement(2386..3069)
                     /locus_tag="PLF20_04355"
     CDS             complement(2386..3069)
                     /locus_tag="PLF20_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOX80274.1"
                     /translation="MSFYFQGVTLGITLAFLMGPALFVLLQTSLNRGLKAGLFIALGI
                     FLSDVSVLTLCLAGFSKIFTQELDQNVFFKILGGIVLIVFGLWTFTRHANVVPEEEND
                     GPPINLKIPGPAIYILKGFILNISNPGVWFLWITAVVSTNSAYGAGSYAVYYFFAGTL
                     SMTLATDLLKCFIADKISIKLNSKVITWTNRIIGLLLMGFGLLLGLSCVINLSHFVPA
                     YGVYNIHNL"
     gene            3558..4079
                     /locus_tag="PLF20_04360"
     CDS             3558..4079
                     /locus_tag="PLF20_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80275.1"
                     /translation="MIRKHLRFSLFLMIFFILCLSFTGCKRPLVKEHVFTNQTWQKFD
                     TIVFNIPSSGDNKPYNIRLFIHYLGAFPTFDLPIVAIMQSPTGEERFFTKRIWLKSLD
                     GKPKGVEADDHFVLEQVLWHEFVFKEKGVYRLIIESDHPNYQVEGIISVKIEILPGAL
                     EPPKPQPGNFNSL"
     gene            complement(4074..4583)
                     /gene="mreD"
                     /locus_tag="PLF20_04365"
     CDS             complement(4074..4583)
                     /gene="mreD"
                     /locus_tag="PLF20_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOX80276.1"
                     /translation="MKTYIKHILRFLVLVFIQLAIFDHLNFGFYIHPLIYILFLLKLP
                     LNTPRWLLLVLAFVLGTTLDIFQSSPGINAAASVLVAFIRPFSIDLIFPRREFDENTE
                     PSIDDFGFQRFFVFTLILTFVLHFILFFLEVFCFDELGKILVRSLASAFVTTLLIVLV
                     ELLFFRKKS"
     gene            complement(4591..5865)
                     /locus_tag="PLF20_04370"
     CDS             complement(4591..5865)
                     /locus_tag="PLF20_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006283814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOX80277.1"
                     /translation="MEHLKKDMYLDMELLRFTTAGSVDDGKSTLIGRLLYDSKSIFED
                     QLEAVKNVSNQKGDHYLDLALFTDGLRSERQQGITIDVAYRYFYTPKHKFIIADTPGH
                     IQYTRNMVTGASTANAAVILVDARNGITEQTLRHYYIAYLLGIKSIIVCINKMDLVNY
                     SSEPYFLIQEEIIRISQKLPVANLIFIPISALKGDNVVKPSANTPWYSGQTLMQTLEA
                     IPIYNHSHRGPLRVPVQYVIRPMLDEFHDYRGYAGRIESGTIRKGDKVMIMPSYMLSE
                     VTSVHLGENFLEEAYAPLSVALTLKDDLDLDRGCFICQPEQLPQTSSILEVMICWFNM
                     IPMKLNQLYTIRQSTWEAKCKIIAQHYKINFHTLSEENSQEELKMNEIGRFTLKTSRP
                     MVFDKYSQIRSTGSFILIDDNTNETIGAGMIQ"
     gene            complement(5875..6798)
                     /gene="cysD"
                     /locus_tag="PLF20_04375"
     CDS             complement(5875..6798)
                     /gene="cysD"
                     /locus_tag="PLF20_04375"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOX80278.1"
                     /translation="MGEYKALFTIKMNHLRELEAEAIYIIREVAAQFERPVILFSGGK
                     DSIVVVHLAYKAFWPAPLPFPLLHIDTGHNFHETIEYRDALVKKTGVRLIVGSVQETI
                     DNGKVKEETGYNASRNVLQTVTLLETIEEYKIDAAIGGGRRDEEKARAKERVFSHRDE
                     FGQWDPKNQRPELWNIFNGKKHLGEHFRVFPISNWTELDVWQYIYYERIEMPSLYFTH
                     KRKVFCRDGVWLAYAPFMKLKPDERVEEREVRCRTIGDITCTGLSLSKASSLEDIISE
                     IASSRVTERGSRWDDKRSDAAMEDRKREGYF"
     gene            complement(7125..7811)
                     /locus_tag="PLF20_04380"
     CDS             complement(7125..7811)
                     /locus_tag="PLF20_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HOX80279.1"
                     /translation="MVNSSELASLLDFFREIKGNNNREWFVQNRQWYEKVKETLENLT
                     KELIRNVHSFDPSIGNLKPLDCLFRIHRDIRFRQDKSPYKTFAGIYIARGGKKSHYAG
                     YYLHLEPTACMAGGGLYNPPTDILKAARQEILYNHDAFEQILRNSTFRKYFEGFSDMG
                     RTKRPPKGFPENFADIEILKNKHFTVARLLSDEEIIESDFPDKVLEIFKALQPLNHFF
                     NGPVEDIILS"
     gene            8023..8841
                     /locus_tag="PLF20_04385"
     CDS             8023..8841
                     /locus_tag="PLF20_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80280.1"
                     /translation="MKRNFRYVFLLLLILPSSLLSQGWEGINGTAAASMDISGVSYPG
                     EWSGFSNPAGLAFISKAYASLSFENRFQMKELTLKGVSIGMPALGGVAALSGVHFGNS
                     LYSEQRYNLAYSKRLGSAFYSGISIDYCMLSQGNGYGNASGLTFGLGLLTGLNENLML
                     GAYVFNPVKIRLSKNIQSEVPVMMKLGLLYSFTRELIATAEVVKNSECKAAVQLGVEY
                     NAFGTFAFRAGVSSWPGSFAFGIGYHPKHLKLDVGAGFHPDLGLCPQAGLIYSF"
     gene            8863..10944
                     /locus_tag="PLF20_04390"
     CDS             8863..10944
                     /locus_tag="PLF20_04390"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOX80281.1"
                     /translation="MSRKLKFIYFWLLMMMQPILHAQHPDTLTRNLEYQIEWFIENTE
                     IELDFSELTEEIEKLVANPVNINSRDREELRRLFFLNEVQIQSILAYTANYGDFVSIY
                     ELKALQGFDPTVIRKIIPFIKIAPFEERRLQLRDAITRGEHSILMRYQRILEKQEGYL
                     PVSDSLLEIKHNARYLGSPDKILLKYGYDYRSQIRWGFLAEKDAGEEFFRGTQKQGFD
                     FYSAYIYGRDLGKIHKMVVGDFHATFGQGLVMWSGLSFGKSGITGGMPHTARGLTPST
                     SSNEIYYLRGGGITLGLGNFEISTLASRIALDASTSVLDTLEPSEIIEGLRTTGLHRT
                     ISEVDLSKNIHLTTLGGNISYTGNIFKAGFTIASENFDKAFNYQTGLYNLFQQPRKSQ
                     FTIGTDVCIYLRDFVLQGEIARDVEGHLAGIFNANFQPDPRLTLAFIYRNYDKAFQNY
                     HSNAFSENSGVYNEQGVYIGFRASVAKQLELTGYADYFLFPWFKYRVDGPSKGREYFI
                     RMDLLMGEKTRAYLRYRFEQKEINTKEENRKLNTLSPRKYHNVRMHFDYQPIEEWKFA
                     SRAEAIFNYTLEGNSRMGFLIYQDVTWQPFRLPISISGRYALFDTETYDERLYAYEHD
                     MLYSFSIPAYYYKGQRIYLILKYSLYKNLNFWLRFASSIYWNKDEIGTGLEIIHGNTR
                     SEIKAQLIWKF"
     gene            11071..12624
                     /locus_tag="PLF20_04395"
     CDS             11071..12624
                     /locus_tag="PLF20_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HOX80282.1"
                     /translation="MDKVKILWVDDEIDLLKAHVIFLEQKGYEVHTSNNGNEALDMMR
                     NVPYDIVFLDENMPGLTGLETLEIIKQINPNLAVVMITKSEEESIMEEAIGSNISDYL
                     IKPVKPNQILLSIKKNLEKKDLVSQKTSTAYQQAFSEIGMEISSSLDFEGWMEIYRRI
                     INWELRLQTTRDEGMAEVLRMQKEEANQVFSRFYETNYPVWLTGKSTSKPVLSHTLIR
                     QKLFPLLEHGSPLFFILIDNLRYDQWKTIQPVIEEFYRVERDELYYSIIPTTTQFARN
                     ALFSGLLPSEIEKKYPKWWLNDWEEGTKNQYEEELFGELLKRFGRDVKFSFNKILNLS
                     AGRKLVDNLPNLLSNRLNIIIYNFVDMLSHARTEMEIIRELANDEPAYRSLTLSWFQH
                     SPLHDILRFLSMKKIDVAITTDHGSIRVQNAVKVVGDRETNTNLRFKAGKNLNFNKKE
                     CFYMRNPAEIFLPRQNVSTSYIFARSSDFFAYPNNYNYFANYYRNTFQHGGISLEEIL
                     VPFVYLKAK"
     gene            12831..13427
                     /locus_tag="PLF20_04400"
     CDS             12831..13427
                     /locus_tag="PLF20_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOX80283.1"
                     /translation="MLKSQQTIQLTPPDLIRGLPAMKTLSLRASATEFDTTMLSLKDI
                     SDLLWAANGINRPTSGKRTAPSAMNAQDIDIYSFMKSGVYLYDAGKHALQLVVTGDHR
                     RLFQRDEKSPAPPVILLLVSDVSRFRAGEDSLKMIWGAMDAGIVSENISMFCASENLA
                     TRPRASMNQKKLRELLNLKDSQYPMLNHPVSYKKNRTN"
     gene            complement(13779..>14150)
                     /locus_tag="PLF20_04405"
     CDS             complement(13779..>14150)
                     /locus_tag="PLF20_04405"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80284.1"
                     /translation="NSVADIAIGKDGEVFIGTSLGIVGFRGDSPSPGPPSDEVYAFPN
                     PVRPSYDGPIGISGLPQNADVKITTISGKLVYHTVAAGTQAVWNGRDFDGRRVQSGVY
                     LVFVSNEDGSEKLITKILFIH"
BASE COUNT         4312 a         2847 c         2778 g         4213 t
ORIGIN      
        1 gcatgtcgag gtggttggtg ggttcatcta aaacaaggag atttacgggt tcgagaagca
       61 gtttagccaa ggcaagtcgg gctttctcgc ctcctgaaag cacctcgact tttttttcga
      121 tggcttctcc actaaacaaa aagctcccca atatagattt caaccttgtt cttatttcac
      181 cctttgccgt ttcgtctaag gtctcgtaaa cggttttgtt cagatcgagc agttccgatt
      241 gattttgggc ataatagcca attttaacat tgtatcccag gcgaaaaata ccttcataat
      301 cgaggcctgc cgcaatgatt ttcgataagg tggatttccc ttccccattt cttcccacaa
      361 aagccacttt ttcgcctttc acaagggtaa aatcgatatt ttgaagcaca actttgttgt
      421 cgtacttttt actgagattt tttatttcta caacgatttt gccacttgaa ggaggaggcg
      481 gaaaacggaa atgaatactg gatcgatcaa ggccgtcgat ctctatttta tccatttttt
      541 cgagcaactt gatgcgcgac tgcacctgac gagccttggt agccttataa cgaaaacgct
      601 cgataaacct ttcgatttga gcaatttgcc tctgttgatt ttcataggct gcaagctgtt
      661 gttcaagctg ttcttctctt attacgatat attccgaata gctcgattta aaatcataaa
      721 tttttccctg actgatttcg atggtgcgat gggttacatt atcgaggaaa gccctatcgt
      781 gcgaaaccac tacaagagcc ccctgaaagt tttgtatcca ttcttcgagc cacgtaattg
      841 cttcgatgtc gaggtggttg gtgggttcat cgagaagaat gaccgaagga ttacgcagaa
      901 gaattttggc cagttcgact ctcatttgcc agccactact aaaagtattc acaggccgct
      961 gaaaatcttc ttccagaaat cccaagccct taaggactct ttcggtttcc cccttcatgg
     1021 cgtttccacc caaaaggttg aacaaatgat tgctttcttc aaaacgctga atgagacgac
     1081 tgtatgactc cgattcataa tccgtgcaca ccgtcaactg gtctgtaagt tggtcgattt
     1141 ctaccttgag tttcaatatg tggtcgaaag ctgtttgggc ttcttcaatc acagttcgct
     1201 ctgattcgat ggtcatatct tgaggtagat ggccaatact cgtgttctga gaatagatga
     1261 tctgaccatt atcgggtgaa atttcccgct tgattatttt taagagagtc gatttgcccg
     1321 ccccattctt tcctaccaga ccaattcggt catgatctgc aatgataaag gaaatatcat
     1381 ccagcaggtt ttcaccggta aaatgaatag agatgtggtt gagcgaaatc atcaattaga
     1441 cagaaaataa taactttttg aaaacgagag cggaacagga ttttctcgat gttccgccct
     1501 tttatgtgat ccatgggttt tgcgaccccg gagggattca aacccccgac tttcagaacc
     1561 ggaatctgac gttctattca gctgaactac ggggccattt tcttgtgcaa aattacatct
     1621 taaccttgtt gagtgcaaat aaggtaatgg atatgaaaag attcttcctt ccctaatatt
     1681 ctgtagccaa catcctttcc cctaacatag ctctaatctc ggagagaaat tgatgaaaaa
     1741 ttatcacatt gaattaaata ataagcttga cttcaacagc tataaagaaa aatctcgtca
     1801 aactcatgaa aggcaatttt ggagtagttg gccaagtcaa taagggttta attcctttta
     1861 taatacctta aagaaaggat gaaatcaaaa acaaaggtta agacctatat gatatcaccg
     1921 aaaatttcga aaaaactcat gataatgtga cgaaccgaca atcaggaaga ggcattcctc
     1981 aatcaaatat tctgcctgaa aattcagctt agtcgattaa cattttaact accgtggatg
     2041 atttgctaaa acgcaaggcg acgatgacgc tcatactcaa aataagttac cgtgccgagg
     2101 cgatcatgaa gcgcagaatg gcagtggaaa aaattaggta aaaaccttac tttctctatt
     2161 gacctcataa tgcccgaaat gtatggtttc gaagctgcca gaatcatcaa tgaaattata
     2221 agagcatgca aggaggagaa aaaggtcaga atcaattctg ttacaagcca ttgctacgaa
     2281 aaaagacaag taaaaaagtc tgaattcagg aagtgagaat tatatgggta atattttaga
     2341 accttctaat ctgcaatgaa ttattgagga tattccggaa cgagatcata agttatgaat
     2401 gttataaacg ccataagccg gaacaaaatg actgaggtta atcacacagc tcaaacctaa
     2461 caataagcca aatcccatta acaacaatcc tataatgcga ttggtccatg ttataacttt
     2521 tgaattgagt ttgatactga ttttatcggc aatgaaacat ttgagcaaat ctgtcgcaag
     2581 ggtcatagaa agcgtaccag caaaaaaata gtaaacagca taactacctg ctccataagc
     2641 cgaattcgta cttactaccg cggtaatcca taaaaaccag acgcccggat tgctgatatt
     2701 tagaataaat cccttcaaaa tgtaaatcgc tggtcctgga attttgaggt tgatgggcgg
     2761 accatcattc tcctcctctg gcacgacgtt ggcatgtcgg gtaaaagtcc ataagccaaa
     2821 aactattaga acaattccac ctaaaatctt gaaaaacaca ttttgatcga gttcctgcgt
     2881 aaaaatcttc gaaaatcctg caagacataa ggttagtaca ctaacatcac ttaaaaaaat
     2941 ccccaaagca ataaataacc cagcctttag tcccctgttc aggctggttt gcaacaaaac
     3001 aaataaggca ggccccatca aaaaagccag ggttatccct aatgtaacac cttgaaaata
     3061 aaacgacata ccctatgata ccacttttat ccttaattgg tgaaaacaac aacgaatttg
     3121 ccaatcagat attttatagt cattcaataa gtattactcc caaaattcat cttttatttc
     3181 ttgggttatc cgttctcata ctaatgttgt cagtatctcc taacttatac aattgcaatt
     3241 catttttcag acatcaaaag caatcttctg taaataataa attcttaaaa attccacaga
     3301 atcctctttt atgttttctt tccttcatgt atgcgaatgg aaggaaaaat ttcacaatac
     3361 ccagagatat ccacaatttc aaataagctg tttccaaatt tggatgaaaa atattgctcg
     3421 agaatttaaa aaaattataa tcacctgata aacaattttt tatcacttcg actatgtgtc
     3481 tatttgagat ttgaacaatt ccccaacacc aaaggcgtta tgtgttataa tccaaatatt
     3541 ataaatcgtt accatatatg attagaaagc acctcagatt ttctctgttt ctaatgatat
     3601 tctttatttt atgcctttct tttacgggtt gtaaaaggcc cttggtaaag gagcatgttt
     3661 ttaccaacca aacctggcaa aaattcgata cgattgtttt caatattcca tcttcgggtg
     3721 acaataaacc ctataatata aggcttttta ttcattacct cggtgccttc cctacattcg
     3781 atcttccgat tgtagctatc atgcaatctc ccacgggcga agaaagattc tttacaaaaa
     3841 gaatttggtt gaagtcgctc gatggcaaac caaagggggt cgaggccgat gatcattttg
     3901 ttctggaaca ggtattatgg catgaatttg ttttcaaaga aaaaggtgta tatcgcttga
     3961 ttatcgaaag cgaccatcct aattatcagg tagaagggat tatttcggtg aagattgaaa
     4021 tactgcccgg agctttagaa cctcccaagc cccaacccgg aaattttaac agcttatgat
     4081 ttcttccgaa aaaataatag ctcgaccaat acgataagca gggtggttac gaatgcactg
     4141 gctaaggaac gtactaaaat tttacccaac tcgtcgaaac aaaaaacttc gagaaaaaaa
     4201 agaataaagt ggagaacaaa ggttaaaatc agagtaaaaa caaaaaatct ctgaaaccca
     4261 aaatcatcaa tggaaggctc ggtattttcg tcgaattcgc gacgaggaaa aatcaaatcg
     4321 atactgaaag gacgaataaa ggctactaat accgatgcgg cagcatttat ccccggactg
     4381 ctttgaaaaa tatccagggt agttcccagc acaaaggcca gaacgagcaa aagccagcgg
     4441 ggtgtattaa gcggtagttt caaaagaaaa agaatataaa taagtgggtg gatataaaat
     4501 ccaaaattta gatggtcgaa tatggccagt tgaatgaaga caagaaccag aaaacgtaat
     4561 atatgtttga tataagtttt cataatgagc ttactgaatc attcctgctc caatggtctc
     4621 attggtatta tcgtcgatca ggataaagct tcctgtgctg cgaatttggg agtatttatc
     4681 gaaaaccatg ggtcgagatg ttttgagggt aaatcttcca atctcattca tcttcaattc
     4741 ctcttgtgaa ttttcttcgg ataaagtatg aaaatttatt ttatagtgct gggcaataat
     4801 cttgcattta gcctcccatg tgctttgccg aatagtgtat agctgattca gtttcatagg
     4861 tatcatattg aaccaacata tcataacctc gagaatagag gaagtttggg gtagttgctc
     4921 gggttggcag atgaagcagc ccctatcgag atcgagatca tctttgagcg taagtgccac
     4981 cgaaagcgga gcataagctt cttcgagaaa gttttcgcct aaatgaacag aggttacctc
     5041 cgacaacatg tacgatggca taatcataac tttatcacct tttctgatag taccagattc
     5101 tattcttccg gcgtacccac ggtaatcatg aaattcatcg agcataggcc ggatgacata
     5161 ctgcacagga actctcaagg gcccccgatg agaatgattg tatatgggaa tagcttccaa
     5221 ggtttgcatc agggtttgac cggaatacca gggggtgttg gcagaaggtt ttactacatt
     5281 gtctcccttt agtgcactga tggggataaa aatcaggtta gcaacaggta atttctggct
     5341 gatacgtatt atttcttctt gaatcaggaa ataaggctca gacgaataat ttaccaggtc
     5401 catcttattg atgcatacga ttatgctttt tatacccaga aggtaggcga tatagtaatg
     5461 tcggagagtt tgttcggtga tgccatttct ggcatcgacg agtataacgg cagcattagc
     5521 ggttgatgct cctgttacca tgttgcgtgt atattgtata tggcctggtg tatcggctat
     5581 gatgaatttg tgtttggggg tataaaaata acgataagct acatcaatag taattccttg
     5641 ttggcgttcg gatcgcagac catcagtaaa gagagccaaa tcgaggtaat gatcgccttt
     5701 ctggttgcta acatttttaa cggcttccag ctgatcttcg aaaatagatt tactgtcgta
     5761 taataagcgc cctatgagag tacttttccc atcatctaca ctgcctgccg tcgtaaaacg
     5821 aagcagttcc atgtcaagat acatatcttt ctttaagtgt tccatagcgt aaaattaaaa
     5881 atagccctcg cgttttcggt cttccatggc agcatccgaa cgcttgtcgt cccagcgtga
     5941 gcctctttcg gtaactctcg aagaggcaat ctctgagata atatcctcca aactactggc
     6001 tttcgacagg gaaagccccg tacaggtaat atcaccaata gtgcggcagc gaacttcacg
     6061 ttcttcgacc cgttcgtcgg gtttcagttt cataaatggc gcataggcca accaaactcc
     6121 atcacgacaa aaaacttttc ttttatgcgt aaaatataaa ctgggcattt ctattctctc
     6181 gtaataaata tactgccaca catccaattc agtccaattg ctgatgggga aaaccctgaa
     6241 atgttcaccc aaatgttttt tcccattgaa gatattccaa agctcgggtc gttgattttt
     6301 tggatcccat tggccaaact catcgcgatg tgaaaaaacg cgttccttgg ctcgggcttt
     6361 ttcttcgtca cggcgccccc ctcctatggc agcgtcaatt ttatattcct cgatggtttc
     6421 cagtagggta acggtttgaa gcacattgcg actggcatta taacctgttt cttccttaac
     6481 ctttccattg tcaatggttt cctgcactga cccaacaatc aaacgaacac ccgttttttt
     6541 tacaagggca tccctatatt ctattgtttc gtgaaaatta tgtcccgtgt cgatatgcag
     6601 aagaggaaaa ggcagaggag ccggccagaa ggctttataa gcaagatgga ctacaacgat
     6661 agagtctttc ccacctgaaa aaaggatcac aggtctttcg aattgagcag caacttcgcg
     6721 aatgatatag atggcttcag cttcgagctc cctcaaatga ttcatcttaa ttgtaaataa
     6781 ggccttatat tctcccataa taaaatttcg tatgcaaatt tacgaaacca aaaggcttca
     6841 aaagtatatg atgattcaga aatgggttat aggtaatttt aaagacaaga gattggaaga
     6901 aatgccaatg cctgaacatg gttagccgtt aaagacaaat ctaatttttt tggatgtaga
     6961 ggccctcgaa gcctccatct tatcattccc atccaaaatc tatttctatc ataaaatgcc
     7021 ttgtaaatca attctatagc taaagtcgga aaacttcccc aaaggtctat ttcactcatt
     7081 atcaacactc caaacgcacc acgccgatca aggtaatcct tcgttcagct cagaatgatg
     7141 tcttcaacag gtccattgaa aaaatgattc aatggctgta aggctttgaa tatttctaat
     7201 actttatcag gaaaatctga ttcaataatt tcttcgtcag aaagcaggcg agccaccgta
     7261 aaatgtttat tctttaaaat ttctatatct gcaaaatttt ctggaaatcc tttgggtggc
     7321 cgctttgttc tacccatatc gctgaatcct tcgaaatact tacgaaaggt ggaatttcgt
     7381 aaaatttgtt caaaagcatc gtgattatat aaaatttctt ggcgagctgc cttcagaata
     7441 tcagtcggag gattgtataa acctcctcct gccatacatg cagttggttc taaatgcaag
     7501 taatagccag cataatgact ttttttgccc ccgcgcgcaa tataaatgcc cgcaaatgtt
     7561 ttataaggcg atttatcctg cctgaacctt atgtctctat gaatccggaa taaacaatcg
     7621 agaggcttta aatttccgat agatggatca aaagaatgaa catttcgaat caattccttt
     7681 gtgaggtttt ccaaggtctc ttttactttt tcgtaccatt gtcggttttg cacaaaccat
     7741 tccctattat tgttgccttt tatttctctg aaaaaatcga gcaaagaggc cagttcagac
     7801 gaatttacca ttttctgtaa atttttacaa aattagacgc ttcttgttgg tgaaacataa
     7861 catactcgac agaattaaat cgcatttatt atcaattttt attgttttac aaagtttcca
     7921 tatattagag ctttgaaatt tatcaaggtc aaaccgcaag gaaaggagtt aaattggcta
     7981 cgaataaata ctatgggtag taatctacaa tagcatcaac caatgaaaag aaatttcagg
     8041 tatgtgtttt tattgttatt gatattgcca tcgagcctgc tatcgcaagg atgggaaggt
     8101 ataaatggta ctgcagcagc aagcatggat attagcggag taagttatcc gggagagtgg
     8161 tcaggatttt caaatccggc gggtttggcg ttcatttcga aagcttatgc cagcctttcc
     8221 tttgaaaatc gtttccagat gaaggaattg acattgaaag gtgtaagtat tggcatgcca
     8281 gcgcttggcg gtgttgctgc tctttcggga gtccatttcg gaaattctct ctatagcgaa
     8341 caacgttata atcttgcata ttccaaaagg cttggttctg ctttttattc gggaatctca
     8401 atagattatt gtatgctatc gcagggcaat ggatatggaa atgctagcgg cctaaccttt
     8461 ggactgggtc ttttgactgg tttaaacgaa aacctgatgt tgggagctta tgtgtttaat
     8521 cctgtaaaga ttcgtctttc taaaaatatt cagtctgaag ttcctgttat gatgaagttg
     8581 ggattgcttt attctttcac cagagagctg attgctacgg ccgaagtggt aaaaaattcc
     8641 gagtgtaaag ctgctgtgca attgggagtg gaatacaatg ctttcggaac ttttgccttt
     8701 agagctggag tttcctcatg gcctggttct ttcgcctttg gcattggcta tcatcctaaa
     8761 catttgaagt tggatgtggg tgcaggcttt caccccgatc ttggtctatg tcctcaggct
     8821 ggattgattt acagttttta atccaatctc aatccattca tcatgagcag gaaattaaaa
     8881 ttcatatact tctggcttct tatgatgatg cagcccattc ttcatgctca gcaccctgat
     8941 accctcacga ggaatctgga atatcaaatc gaatggttta tagagaatac cgagatcgaa
     9001 ttggatttta gcgagcttac cgaagaaatc gaaaaacttg tagctaatcc tgtcaatata
     9061 aatagccgtg atcgtgagga attacgacgt ctttttttct tgaatgaagt acaaattcag
     9121 agcattttgg cttatacagc caattatgga gattttgtga gtatctacga gctgaaagcc
     9181 cttcaaggat ttgaccctac tgtgatcaga aaaataattc ctttcataaa gattgcacct
     9241 tttgaagaga gacgacttca actgagagat gcgataacca gaggagagca ttctattttg
     9301 atgcgttacc agcgaatcct tgaaaaacag gaaggatatt tacctgttag tgacagtttg
     9361 ttagaaataa aacacaatgc gcgatatctg ggaagtccag ataagatttt actgaaatat
     9421 ggctatgatt accggagtca gatacggtgg ggatttctgg ccgaaaaaga tgccggagaa
     9481 gaattttttc gaggaacaca aaaacaaggt ttcgattttt attcagctta catctatggc
     9541 cgggatctcg ggaaaattca caagatggtc gtgggagatt ttcatgccac ctttggacag
     9601 ggtttggtga tgtggagcgg tttgtcattc gggaagtcag gaattacagg tggtatgcct
     9661 catactgccc ggggtcttac tccaagcacc tcatccaatg aaatttatta tttgagaggt
     9721 ggaggaatta cgttaggact gggcaatttc gagataagta ctctggcttc acgtattgcc
     9781 ctcgatgcga gtacatcggt actggatacg ttagagccat ctgaaatcat cgagggactt
     9841 cgaactactg gcctgcaccg cacaatatct gaagtggatc ttagtaaaaa catacatctt
     9901 actactcttg gaggaaatat cagttatact ggaaatattt tcaaggccgg ttttacaatt
     9961 gcatcggaaa atttcgataa ggcattcaat tatcaaacag gcctttacaa tttatttcag
    10021 caacccagaa aatcacaatt caccataggg actgatgttt gcatatattt acgcgatttt
    10081 gttctacaag gcgaaattgc cagagacgtg gaagggcact tagcgggaat tttcaacgct
    10141 aattttcagc ctgatccacg actcacattg gcgttcatat accgaaatta tgacaaggct
    10201 ttccaaaact atcacagcaa tgcttttagc gaaaattcgg gtgtatataa tgaacaggga
    10261 gtatacatag gatttagggc atctgtagcc aaacaacttg aattgacagg ctatgccgac
    10321 tatttccttt tcccatggtt caagtatcgg gtcgatggcc cttcgaaggg aagggaatat
    10381 ttcattagaa tggatttatt aatgggcgaa aaaaccagag cttatctccg ctacagattc
    10441 gagcaaaaag aaattaatac gaaagaggaa aaccgaaaac ttaatacact ttcccctcgt
    10501 aaatatcaca acgtaagaat gcatttcgat tatcaaccca ttgaagaatg gaagtttgcc
    10561 tcccgagcag aagccatttt caattacact ttggaaggaa attcaagaat gggatttctt
    10621 atttatcagg atgttacctg gcaacctttc cgacttccca tttccatctc gggtagatat
    10681 gctctcttcg acacggaaac ttacgacgag cgtttgtatg cttatgaaca tgacatgcta
    10741 tacagcttct ccattccggc ttattattat aaaggacaga gaatctattt gattttgaaa
    10801 tattccctct ataagaacct taacttttgg ttgcgttttg cttcatccat ttattggaat
    10861 aaggatgaaa ttggcacggg tcttgaaatt attcatggaa acactcgaag cgaaatcaaa
    10921 gcccagctta tctggaaatt ttagtcattt atcagagcgt gaaataaact tttttgaaag
    10981 ccgttgatat atttatcggg ctaatgattt gtgaaggctt tgtgctacaa taataaaata
    11041 ttttcccttc attataaatg gagatagttt atggataaag tcaagatact atgggtggat
    11101 gatgaaattg atctgttgaa ggctcatgtc atatttttag aacagaaggg ctatgaggtg
    11161 catacttcca acaatggcaa cgaagctttg gatatgatgc gcaatgtacc ttacgacatt
    11221 gtttttcttg atgagaatat gccgggactc acgggtctgg aaacgctcga aatcatcaag
    11281 caaatcaatc ccaaccttgc cgtggtgatg attaccaaaa gcgaagaaga aagcataatg
    11341 gaagaagcca ttggaagtaa catctctgat tacctcatta agcccgtaaa acccaatcag
    11401 attctattat ccatcaaaaa aaatcttgaa aagaaagact tggtaagtca aaagacctca
    11461 acggcttatc agcaggcttt tagcgaaata ggtatggaaa taagtagttc gctcgatttt
    11521 gaaggttgga tggagattta tcgccgaatc atcaattggg agcttcgtct acaaactact
    11581 cgtgatgaag gcatggcgga ggttctgaga atgcagaaag aagaagcaaa tcaagtgttt
    11641 tctcgttttt acgaaacaaa ttatcctgtg tggttaaccg gcaaatcaac cagcaaacct
    11701 gttctttcgc atacgcttat taggcagaaa ctcttccctc ttctggaaca cggctctcca
    11761 cttttcttca tattgatcga taatttacga tatgatcaat ggaaaaccat tcagcctgta
    11821 attgaagaat tttacagagt ggaacgtgat gagctatact acagtattat tcccaccact
    11881 acacaatttg cccgtaatgc cttattttca ggacttttac catccgaaat cgaaaaaaaa
    11941 tatcctaaat ggtggctcaa cgactgggaa gaaggcacca aaaatcaata tgaggaagag
    12001 cttttcggtg agctgctcaa acgattcggt cgcgatgtca agttttcgtt taataaaatc
    12061 cttaatctgt cggctggacg caaactcgtg gataatctcc ctaatctcct ttccaatcgc
    12121 ttgaatatca ttatttacaa ttttgtagat atgctttctc atgcccgtac cgaaatggaa
    12181 attatacgtg aacttgccaa cgatgaaccc gcttaccgct ccctcactct ttcatggttc
    12241 caacattctc ccctccacga tattctgaga ttcttatcca tgaaaaaaat cgacgtggcc
    12301 atcactacag atcatggttc tatcagggtt caaaatgctg ttaaagtagt gggcgacagg
    12361 gaaaccaata ccaatctgcg attcaaagct ggaaaaaatt tgaatttcaa taaaaaagaa
    12421 tgcttttata tgcgcaatcc tgcagaaatt ttcctccctc gccaaaatgt gagcacttcc
    12481 tatatattcg cacgaagcag cgattttttt gcctatccta acaactataa ttatttcgcc
    12541 aattattacc gcaatacttt ccagcatggc ggtatatcgc tcgaggaaat tcttgtgcct
    12601 ttcgtatatc tcaaggcaaa ataaagaggg taagaaatga ttcaaaagag tttttctatt
    12661 cgcttcgaat tatttttaag acggcaacat aatttttatg tatatttacg aaaactttca
    12721 aggtgactac cttatttctt aaattctaaa cgtttatctc aaaccttttg aatatgaata
    12781 agattgctca ttatgtttca ttttcagttt tgtttttact gacctcaacc atgttaaaaa
    12841 gtcaacaaac tattcagctc acgcctcccg atcttatcag gggtcttccc gccatgaaaa
    12901 ccttatcgct tagggcttct gcaaccgaat tcgatacgac catgctttcg ttgaaggata
    12961 tatccgatct attgtgggct gccaatggca taaaccggcc aacgtcgggc aaacgcactg
    13021 ccccttcggc tatgaatgcc caggatatcg acatttattc ctttatgaaa tcgggagtat
    13081 atctgtacga tgcagggaaa catgcacttc agcttgtggt aacaggcgat cacagacgtt
    13141 tgttccaacg cgacgaaaag tcacctgctc cacctgtaat tctgctactt gtttccgatg
    13201 tctctcggtt cagggctgga gaagattcat taaaaatgat ttggggcgca atggacgcag
    13261 gtattgtttc agagaatatt tcgatgtttt gtgcctcaga aaacctggct acccgtcctc
    13321 gagccagtat gaatcagaaa aaactaagag aactgctcaa cttgaaagat tctcaatacc
    13381 ctatgctgaa tcatcctgta tcttataaaa agaatcggac gaattaattc agaatatcgc
    13441 cactaaactc tccctgattc atagctttcc gaacaaaatt tctgtgtaaa ggccgttcct
    13501 acataaaatt tgattattta gggcgaattg gaggatggtg agtagaatgt ggaacgaaat
    13561 gaaaatgaca aataaaggag ttgagtgaaa agcgaaaaag catttcacgg tgagtgattt
    13621 atgaagcagc ttaggtcttt tcgtattacc tattcactga tcatttgaaa taccaaagga
    13681 taattatcat attcgccttc tcgcccattc taccttcttg cagtaatttt attttccagt
    13741 gaataaatct tattcgtttt aaggtctgca aaacattgct aatgaatgaa taaaatcttg
    13801 gtgatcagtt tttcgcttcc atcttcgtta gaaacaaaaa ccagataaac tccgctttgc
    13861 actcttcggc catcgaaatc gcggccattc catacggctt gtgttccggc ggctacggta
    13921 tgataaacta gctttccgct gatagtagta atttttacat cggcattctg cggaagtcct
    13981 gaaattccaa taggtccatc ataagatgga cgtacgggat ttgggaaagc ataaacctca
    14041 tcagaaggag gcccaggtga agggctgtcg cctctaaagc caacgattcc caaagaagtt
    14101 ccaataaata cttccccatc tttgcctatg gcgatgtcgg ctaccgaatt
//
LOCUS       DAOORU010000045        14139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_5270_length_14139_cov_5.894419, whole genome shotgun sequence.
ACCESSION   DAOORU010000045 DAOORU010000000
VERSION     DAOORU010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14139
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5270_length_14139_cov_5.894419"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(557..1672)
                     /locus_tag="PLF20_04410"
     CDS             complement(557..1672)
                     /locus_tag="PLF20_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80285.1"
                     /translation="MKAKIFCLSLLASLFFMVGCGEDDDGTEPTPNGDSTKISPSWTF
                     TEIPELKFAEISRNGKWAIVASDYRMALLDLKNKAELYEVATSESDLDYVVQVSISDQ
                     GYALVASANGIGVISATQGLIANFSEVYIGQARYYVRGYPSMIAPSGVSYYVHGNMKF
                     SLPDNLVWLNTTDSPNYDFSNELDLSGDGSLIASASGVYGKLVLLDGNGNILWKSSIA
                     NDAIRNVDISIDGSTIAIGDLSEIDVYASNSPAPIIRWPHNSYYAIVAVSKNGSVITG
                     GTSHGKLVIFKNGSETPNIYDKPDGQVNDIAISDDGKIIVLATDFGGLEIYNDLGKLK
                     YQHKGQYEGIVSCVSISSDGKNVGCIFQNEFHLYALP"
     gene            complement(1916..2515)
                     /locus_tag="PLF20_04415"
     CDS             complement(1916..2515)
                     /locus_tag="PLF20_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80286.1"
                     /translation="MAAEGGWSTRTAKIADDVPPDFKNYMKDLKTGFHFGMDAYYYFI
                     ENIGIGIKCNRYHSKNVLKNVYIQYANGSMEFGNMSDNIKIYFIGPFVSLRMLNKNKN
                     NNFYMNWGIGYTQYNDEAVFVYEFSTKGNNIGLFADWGYEIGLSKNFSLGFQLSIIAS
                     TLTKYKLITDFHTETVKLDKENYENLSHLNLSIGLRFYK"
     gene            complement(2822..3724)
                     /locus_tag="PLF20_04420"
     CDS             complement(2822..3724)
                     /locus_tag="PLF20_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80287.1"
                     /translation="MIRNIYLKNQRKVNFILLLGSLILVSGCAPKISNKISKQYPALD
                     YREEVRVFNLDESLPPDAEELGKVNINDNGFSANCGWSEVIELAKTEARKIGGNAIKI
                     TKHRPPDFTSSCHRIDALIVRIENLDQQKITEVLDTSLISADYALLRIYRNNSYGTLI
                     RYDLYLGDSLIARVANNWRKTVKVKKDGYNTLWAKTEAKEELPIKIEKGKEYYIRCGV
                     RPGLMIGRPELELVDNQRGKSEFGLIKAKNEITQILMNDGSIIKCYINYEDNDNLYIT
                     DINDKQNQEKFIRKSEIKKIDKKN"
     gene            complement(4085..4987)
                     /locus_tag="PLF20_04425"
     CDS             complement(4085..4987)
                     /locus_tag="PLF20_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOX80288.1"
                     /translation="MEKKTSSISLKTLFYLYLFIFGGLAIIGSIVIVLMVYLFKTMNF
                     ADILSFAQNAYHSGLLLFVLFGFFAQMIDGTLGMAYGVSSTSFLISTGISPAIASASV
                     HAAEIFTTGISGISHWRFKNLDKKLFIQLAIPGVIGSALGAYVLSSFQGDILKPIINV
                     YLLLMGFRILYSALRKKIMARKFKQYTVLGLLGGFVDASGGGGWGPVVTTTLVGTGET
                     PRVTIGTVNTAEFLVTLASTGVFTMVIGLSHWHIIVGLILGGMIAAPLAAFLCNKINA
                     RWAMVLVGILIMALSIRTLLQIIF"
     gene            complement(5349..8030)
                     /locus_tag="PLF20_04430"
     CDS             complement(5349..8030)
                     /locus_tag="PLF20_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOX80289.1"
                     /translation="MTTRDWYSKSPQEVLKKLNVDPSAGLSEEEAKKRLEEFGPNKLQ
                     EQKKKSIFQLFVAQLNDWLIYVLFAAVIITLLMGEYIDAIIIMAVILLNAILGVVQEV
                     RASNAIEALQRMTRHKALVKREGQIREVDSEGLVPGDIVILDTGRFVAADLRLIETIN
                     LQIEESALTGESVPSEKEASLILDPKTPLGDRENMAFMSSLVTYGRGEGVVIATGMNT
                     EVGKIVGIINKEEPLKTPLEIRLNQLGKVLGKLAIGICIFIFIVGILQGRDLAEMFLI
                     SVSLAVASIPEGLAAIVAVVLSVGVTAMSKRNAIIRRLPAVETLGSVNIICTDKTGTL
                     TQNKMTVLQSFTLDERKDIQRGQINYSKDSTILLAKAMILSSDATWDGQMGTGDPTEI
                     ALLMFGDDVGLDRTKLEKDTRRIGELAFDSERKLMSTLVEEDGKLTVYTKGAIDNLLG
                     ICTHVVANGEVLPMTDQWKEEFNQAVIRMTASALRTLGAAYKPVDKPTENSEMEKDLI
                     LIGIVGLMDPPRPEIKHSIEKAKKAGITTVMITGDHKNTAFAIAKELGIAETIQQVIT
                     GLEIDEMSEEVFSEKIMQYRVFARVSPEHKVKIVHAFQSHGYIVSMTGDGVNDAPSLN
                     AADIGIAMGVTGTDVAKDSSDMILTDDNFSTIVLAIEQGRNIYNNIRKAVLFLLTCNL
                     GEVITMFVALISGMAAPFLATQLLWINLITDTLPALALGIDPPDPDIMEEKPRDPNES
                     FFAHGEGLRIILGGILIGSISIIAFWFGYFEHGFSPFSQNVPQKVIEYARTLAFITII
                     ACQLIYSLGLRSNKKSIFSVGIFSNKYLAGSIILGLALQLLVVFIPFLRNAFKLQMLD
                     LQGWLIAIVLGLFPLFVNELHKLILRLKEKRTLQQLI"
     gene            complement(8361..8432)
                     /locus_tag="PLF20_04435"
     tRNA            complement(8361..8432)
                     /locus_tag="PLF20_04435"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8398..8400),aa:Met,seq:cat)
     gene            8713..9591
                     /gene="era"
                     /locus_tag="PLF20_04440"
     CDS             8713..9591
                     /gene="era"
                     /locus_tag="PLF20_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOX80290.1"
                     /translation="MNHKSGFVNIIGKPNVGKSTLLNALVGEKISAVTYKAQTTRHRI
                     LGIVSTDDYQIVYSDTPGILDPAYKLHESMMKYVETSLEDADVFLYMVEAGERNPPLN
                     IIQRIKESGIPIILLINKIDKIKPEQLGEEMSFWKEYFPQADILPISALYSSQLPELV
                     NKVVELLPENPPYFPKDEITDRTTRFIVSEIIREKIFLNYRKEVPYHSEVVVDSFKED
                     DNFIRIRAVIYVARESQKMIIIGKNGEAIKKMGIEARKDIEDFVGRKVYLELSVKIAE
                     NWRNDPRMLRRLGYEL"
     gene            9608..10918
                     /gene="der"
                     /locus_tag="PLF20_04445"
     CDS             9608..10918
                     /gene="der"
                     /locus_tag="PLF20_04445"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOX80291.1"
                     /translation="MASNILAIVGRPNVGKSTLFNRLIGARQAIVDPTTGVTRDRNYG
                     QSDWNGINFSVIDTGGYVENSDDIFEEEIRKQARLAIDEADVIVFLTNGRDGITPLDE
                     DLANILRKSGKPVILAVNKIDDPVLVNLGAEFYSLGMGEVYPISAMTGSGTGELLDAV
                     VAHFSSQPETEENEILPRIAVVGRPNVGKSSFINMLTGTERNIVTPIPGTTRDAIHTP
                     FKAFGFDFLLVDTAGLRKKSKVEENIEFYSVMRSVRAIENSDVCILMADANVGFQQQD
                     MNIFSLIQRNHKGMVILMNKWDLVNKDSKTLRIYEEEVHRKIEPFTDVPVLFTSVHEK
                     KRLLKALEWAIKVYENRKQRITTSRLNEVMLPIIGQNPPPVVKGKVVKIKYATQLPKP
                     YPVFLFFCNHPQYIREDYKRFIENKLRENFSFTGVPIEIYFREK"
     gene            10915..11298
                     /locus_tag="PLF20_04450"
     CDS             10915..11298
                     /locus_tag="PLF20_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HOX80292.1"
                     /translation="MTEEEIRIDKWLWAVRVFKTRSLASQACRSGKVRINGMVVKPSR
                     DIRCGDVIQISLGSLNKTVEVKAILHNRISAKEVSTYLIDRTPVEEYERQMIIAEMKQ
                     EIRPKGKGRPTKRERRDIDRLKDSF"
     gene            complement(11281..13893)
                     /locus_tag="PLF20_04455"
     CDS             complement(11281..13893)
                     /locus_tag="PLF20_04455"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HOX80293.1"
                     /translation="MSESAFGLSDSIKSCRAVYFPEAKINLDGIEEESEWKQTNACGN
                     FIQQRPYEKTSPSQTTEFKVIYDDEFIYFFIQAYDSMPKKIERRLARRDDIEGDWAGV
                     QLDTYNDRTTAFTFFVNAAGVKFDGIESETDNMEDVDPTWDAVWEAKTVVNDKGWSIE
                     MKISFSQLRFDKNNTEGWGLQVTRYIHRNEEMSLWQYIPRNAPGWVRYFGRLNGLEHI
                     KRVPQIEAAPYVALQHETYKKEEGNPYRSGKNTLFRAGIDGKVSLASNVILDYTINPD
                     FGQVEADPSVVNLTAFETYFSEKRPFFIEGKNILDFQITPGNNSFSGDNLFYSRRIGR
                     KPQYTPDANYVNIPKATPILAALKLTGKTKDGWSFGALESMTGRTSAEITEGTEENRI
                     LCEPFSNYFVGRIQKDYHQGESRIGGMLTSVFRDHSEGNPGLLPTWATTGGFDLYYSW
                     KKQTYFLDIKAIASLVNGSKESIGRLQQSPIRNFQRSDATYLKLDTSRTSLSGHGGSI
                     HFSRQGNSNWKYSLFVTWRSPELEINDIGYLHQADNIFQVAWVGYEKYHPFLIFRNAG
                     FNLNQWTGWDFGMNSLFKGGNINAWAQFKNYWQISAGLNFDGNNLDNYVLRGGPALKL
                     PGGYSYWGYLSTDSRKKLVAEINASIYRTFIANNNYNFFGVGMHYQPFTALSFGIFPE
                     YSVNNDNLQYVKEKKMNGVPRYIMAYIHQKTLALEMRINLNLSNDLTLQYYGQPFISS
                     GEYSHFKFISSPKANDYYQRFTEYSEAQIFYQPSDKTYQIDEDYDGIPDYSFNKPDFK
                     ALYFVSNLVLRWEYRPGSFIYVVWSQNRADQGKEISFKPKKDITSLFDIYPGNTFMVK
                     ISYLIQYQHLKRIF"
BASE COUNT         4338 a         2724 c         2598 g         4479 t
ORIGIN      
        1 attcaacttg ctgtataaaa ggaaattgca tccctaaggc tctcaagttg ggttacgtga
       61 atccagcttt tttatcttgt cgaataagtc cttggcaagg cagttgatgc tatcattgtg
      121 gctctgattg ttttcttcca tgattttatc gtattaaaga tatagttgga aatttaaaga
      181 ttggttagtg agaaggcagt gtataatttt catttagtaa gttgaaaaaa atctaattga
      241 accttattgt tgaccaaaat aacttgaaat ttttatgtga gccaaagtag taaatttttg
      301 ttatgctggc gtaaaggcat tcaatttttt tatttttcag gaaatgtttt gccgctgata
      361 cattattccc attcaaatct ttgtttcctg caatgttttt aaacaataca gcacattgcc
      421 caccccttga taaaatgctg cttccgaggt gttcaaccga taatttctcg tcttcgtaaa
      481 tttcttcatt ctccttactc cacctcctgc ctctccacat ttactttgta acttgataga
      541 tcaacagcca gtcctatcat ggtaaagcat agagatggaa ttcgttttga aaaatgcacc
      601 ctacattttt tccatctgaa gaaatactaa cacatgacac aataccttca tattggcctt
      661 tgtgttggta tttgagtttt ccgagatcat tataaatttc taaccctcca aagtcggttg
      721 ctaaaacaat tatttttcca tcatcagaga tagcaatatc atttacttga ccatcgggtt
      781 tgtcgtatat atttggtgtt tcagaaccat tcttaaagat gacgagtttc ccatgtgatg
      841 taccgcctgt aattacactc ccattttttg aaacagccac tatcgcgtaa taactgttgt
      901 gaggccacct tattatagga gcagggctat tgcttgcata aacatcgatt tcgctaaggt
      961 caccaatagc gatggtactt ccatcaattg aaatatctac attacgtata gcatcattag
     1021 caatggatga tttccaaagt atattcccat tcccgtccaa taaaaccaat ttaccatata
     1081 ctccactagc gcttgctatc agtgagccat cacccgataa atctagctca tttgaaaaat
     1141 catagttggg actgtcggta gtatttaacc acaccagatt atcaggaaga gaaaatttca
     1201 tatttccatg tacatagtat gaaaccccag aaggggcaat catagatgga taacctctca
     1261 cataataacg tgcctgacca atataaactt ccgaaaaatt ggctatcaag ccttgggttg
     1321 cagaaataac cccgattcca ttcgcgcttg caaccaaagc ataaccttgg tcagaaatgg
     1381 aaacttgcac aacataatct aaatcgcttt cgctggttgc cacttcataa agttcggctt
     1441 tatttttcaa atccaataaa gccattctat aatcactggc tacgattgcc catttgccat
     1501 tacgtgaaat ctcggcaaat tttaattcgg gaatttcagt aaatgtccaa gatggactta
     1561 ttttagttga atctccattg ggagtaggtt ccgtgccatc atcatcctca ccacaaccaa
     1621 ccatgaaaaa taagcttgcc aacaggctaa gacaaaagat tttcgctttc ataatgcacc
     1681 tccttttgtg aattgaatgg taaaattatg gcaaagattt gttcactttt acaaacagac
     1741 aaaaaaattt ataaaaattt tgatttcttc ttgaaacctt aatgaaaata aagttttaag
     1801 caattgaaaa gtttctttat ctcaagaaat tatagaaaat tatatattca gttattattg
     1861 aaaaagaaaa aataaaatag gtttattgta agtaaggaaa gaaaatctac ctgtttcatt
     1921 tataaaatct tagacctatt gataaattga ggtgggataa attttcgtaa ttttccttat
     1981 ccagtttaac cgtttcagta tgaaaatcag ttattaattt atattttgtt aaggtgctgg
     2041 ctataatcga gagctgaaat ccaagtgaaa aattcttaga taaaccaatc tcatatcccc
     2101 aatctgcaaa taagcctata ttatttcctt tagtggaaaa ttcataaaca aaaacagctt
     2161 catcattata ttgagtatat cctattcccc aattcatata gaaattatta tttttattct
     2221 tgttcaacat tcggaggcta acaaatgggc ctatgaaata aattttaata ttgtcgctca
     2281 tatttccaaa ctccatactt ccattagcgt attggatgta aacatttttt aatacatttt
     2341 ttgagtgata acgattgcat tttattccga tcccaatgtt ttcaatgaag taataatatg
     2401 catccatgcc gaaatgaaaa ccagttttta aatccttcat ataattctta aaatcagggg
     2461 gtacatcatc tgcgattttt gctgtacgtg tactccatcc tccttcagca gccaatttaa
     2521 aacgagggaa atcccgtatt atctttattt tatcttgagg gatttcggaa gtggcaaagt
     2581 aattggattg atgatatttt acctgactct ttggcaacaa agtattcctt atttcatttt
     2641 tatacttaaa agtgaaataa ataaagtcgg gttttatttt cgttatttta caatttatcg
     2701 agtcattgtc tattgtaaca attaagtcct gagctattaa aggcagcgaa ttacataaag
     2761 gtataaggaa tatcatacaa acaacatttc gaaaattttt catattaaaa aaatttatag
     2821 tttaattttt tttatctatt tttttaattt ccgattttcg aataaatttc tcctgatttt
     2881 gtttatcatt tatatcagta atgtagaggt tatcattgtc ttcataattt atataacatt
     2941 ttatgatact gccatcattc atcagaattt gagtgatttc attttttgcc tttataagtc
     3001 cgaattcgga ttttcctctc tgattatcaa cgagctctaa ttcaggacgt ccaatcatta
     3061 aacctggacg aactccacat cgtatataat attctttccc tttttcaatt tttataggta
     3121 attcttcttt tgcttcggtt tttgcccata aggtattata accgtccttc ttaactttta
     3181 ctgtttttct ccaattattt gctactctcg caatcaagct atcacccaaa taaagatcat
     3241 atcgaattaa tgtgccataa ctattattac gatatattct gagtaaggcg taatcagctg
     3301 aaattaacga tgtgtccagt acttcagtaa tcttttgttg atccagattt tctattctta
     3361 ctattagtgc atcaattcta tgacaggaac ttgtaaaatc aggtggacga tgttttgtga
     3421 tttttattgc attccctcca attttacgag cttcagtctt ggctaactca attacttcgc
     3481 tccatccaca atttgccgaa aagccattgt cgtttatatt cactttgcct aattcttcgg
     3541 catcaggtgg aagagattca tcaagattaa ataccctgac ttcttcacga taatcaagtg
     3601 ctggatactg tttgcttatt ttattggaaa ttttgggcgc acatccgcta acaaggatca
     3661 aactccctaa aagcagaata aagttaactt tccgctgatt tttaagataa atatttctta
     3721 tcataataaa tttggttaaa atagttttat ggacaggatt taaaaatatc tatttaaatg
     3781 attttaaaat gataatgatt atgaaactga tgatgtttca atgctaagtt cttccttatt
     3841 ttgcccatta attgtttata gcagataact aatataaatt cgggttttgg tgataataat
     3901 tctattattt tgataattga gattggttaa tgaggcaaag atagtatttt ttaataatgg
     3961 agcaaatgat ggtaaagttt atcactttta gtatattttt atgggaagat tatacctctt
     4021 ccataagaat ttttgaatct gctctgtgaa tgtagctttt tactcagcca gttggccagt
     4081 tattttaaaa aataatttga agaagagttc tgatgcttaa ggccatgata aggataccca
     4141 ccaaaaccat agcccatcga gcatttatct tgttgcagag gaaggcagcc agaggagctg
     4201 caatcatacc gcccaaaatg aggccaacaa tgatatgcca gtggcttaat ccaattacca
     4261 tggtaaatac tccagtcgag gctaaggtaa ctaaaaactc ggcggtattc acagtgccaa
     4321 tggtaactcg tggcgtttcg cctgtaccaa ccaatgtggt cgtaaccact gggccccagc
     4381 ctcctcctcc agaagcatct acaaaaccac ctaataaacc caaaacagta tattgtttga
     4441 attttcttgc cataatcttt ttccgtaagg cgctatacag aattcgaaag cccatcaata
     4501 acaaatatac attgatgatg ggtttaagta tatcaccctg aaaggaggac aggacataag
     4561 cacccaaggc tgaaccaatg acgccgggta tggccagttg aatgaataat tttttatcga
     4621 ggtttttgaa tcgccaatga gaaatccctg aaatacctgt agtaaagatc tcggcagcat
     4681 gaacgcttgc actggcaata gctggtgaaa tccccgtact tattagaaaa cttgttgagc
     4741 ttactccata ggccattcct agtgtcccat cgatcatctg tgcaaaaaaa ccaaacaata
     4801 caaataagag aagtcccgaa tgataggcat tttgcgcaaa ggaaaggata tcagcgaaat
     4861 tcatagtttt aaacaggtac accatcaaga ctatgacgat ggaacctatt atggcaagcc
     4921 ctccaaaaat gaatagatac aaataaaaaa gcgttttaag gctgattgaa gatgtcttct
     4981 tttccatttc ttcttgtttt gaaaatgaag gatttcattg ccttccttca aagaaatcct
     5041 ttgtcgaagt cgcaaagata cactgattga ctttacaaat acctatttga aaacaacctt
     5101 ctcgaagttt tgatggttgt tcaattacta tagatggctg tctgcaaaaa atgattcaat
     5161 tatttaatta atcctgcttc gatagaaaaa tcagtctttg acgctctgca gcctgtgcag
     5221 gaaatgttta agcatgataa taatggtatg gggtttggaa acagaaatac taatcagaat
     5281 cttgttattc tgaagagttt cattatatga aattttggta atccatttgg aatcgatagg
     5341 gaatagattc agatgagctg ctggagtgtt ctcttctctt tcaggcgaag gatgagttta
     5401 tgcaattcat tgacaaaaag agggaataat ccaaggacga tggcgataag ccaaccctga
     5461 aggtcgagca tttgaagttt gaaagcattt cgcaaaaacg ggataaatac aaccagcaat
     5521 tgcagagcca aaccaagaat gatagagcct gcaaggtatt tattcgaaaa tattcccact
     5581 gaaaaaatag attttttgtt actccttaac ccaagggaat aaatcaactg acaggcaatg
     5641 attgttataa aagccaaggt tcgtgcatat tctataactt tttgaggcac gttttgactg
     5701 aatgggctga agccatgttc aaaatatccg aaccagaaag ctatgataga aatcgatcct
     5761 ataaggatgc ctcccagaat gatgcgcaag ccttccccat gagcgaagaa actttcgtta
     5821 ggatcacggg gtttttcttc cattatatcg ggatcgggcg gatctattcc taaagccagc
     5881 gcaggcaatg tatccgtaat aagattgatc cacaaaagtt gagtagcaag aaaaggtgca
     5941 gccatccccg aaattaatgc tacaaacatt gtaatcacct ctccaagatt acatgtcaat
     6001 aaaaacagaa cagcctttct gatattattg taaatattac ggccttgctc gattgctaat
     6061 actatggtag aaaaattgtc atccgtcagt atcatatccg aagaatcctt ggctacgtca
     6121 gttcctgtaa ctcccatggc aatgccaata tccgccgcat tcaacgaagg tgcatcattt
     6181 acgccgtcgc ctgtcatcga tacaatgtaa ccatgtgatt ggaaagcatg tacaattttc
     6241 accttatgtt cgggcgaaac cctagcaaat actcggtatt gcataatttt ttccgagaat
     6301 acctcttccg acatttcgtc tatttcgagg ccagttatca cttgttgtat tgtttcggct
     6361 atacctaatt ctttggctat tgcgaaggct gtatttttat ggtcacccgt tatcataact
     6421 gtggtaattc cagccttttt ggccttttcg atagaatgtt ttatttctgg acgaggcgga
     6481 tccatcaacc caacgatacc aatcagaatc aaatctttct ccatttccga attttctgtg
     6541 ggcttatcaa cgggcttata ggctgcaccc aaggttctca aagcagatgc cgtcatcctt
     6601 atcacagcct gattgaattc ttctttccat tggtcggtca taggcaatac ttccccattg
     6661 gcaaccacgt gggtgcagat tcctaacaaa ttatctatgg cgcctttggt atacacagtg
     6721 agcttgccat cctcctccac caaggttgac ataagttttc tctcggaatc gaaagccaat
     6781 tcgccaatgc gccgagtgtc tttttcaagc ttggttctat cgaggccaac atcatctcca
     6841 aacattagca aggcaatctc ggtaggatca cccgtgccca tctgtccgtc ccaggtagcg
     6901 tcggaagaaa gaatcatagc cttggcaagc agtatagtcg aatcttttga ataattgatc
     6961 tgtccccttt ggatgtcttt cctttcgtca agggtaaacg attgaagaac cgtcattttg
     7021 ttttgagtaa gtgtacctgt tttgtcagta cagatgatat tgacagaacc aagggtttca
     7081 actgcaggca atcttcgaat aatggcatta cgtttcgaca ttgccgtaac tccaactgag
     7141 agaacaactg ctactatggc tgcaagtcct tcgggaatgg aggccacggc caatgaaaca
     7201 gaaatcagaa acatctcggc aagatcgcgg ccttgtaaaa tgccgacaat aaaaataaaa
     7261 atacatattc ctattgctaa ttttccaagc acttttccaa gctggttcaa gcgaatttcg
     7321 aggggtgttt tgaggggttc ttctttattg atgattccga caatctttcc aacttcggta
     7381 ttcattccag tagctataac aacgccttct ccacgaccat aagttaccag actgctcatg
     7441 aatgccatgt tttcgcgatc gcctaatggc gttttaggat cgagtataag agaagcctct
     7501 ttttcggagg gaaccgattc tcctgttaat gccgattctt cgatttgaag attgatggtt
     7561 tcaattaacc gaaggtcggc tgccacaaat cgaccagtgt ctaaaatcac gatatcgccc
     7621 ggaaccaaac cctcagaatc aacttccctg atttgtccct ctctttttac taaggccttg
     7681 tgacgggtca ttctctgcaa ggcttcgatg gcgttacttg ccctaacttc ctgaacaact
     7741 ccaagaatgg catttagtaa aatcactgcc atgattataa ttgcgtcgat atattcaccc
     7801 atcaataagg tgatgataac ggcagcaaat aatacataga tcaaccaatc atttaattga
     7861 gctacaaata attgaaaaat gctttttttc ttttgctcct gaagtttatt tggcccaaat
     7921 tcttccagcc ttttttttgc ctcttcttcg cttaatcccg ccgaaggatc tacatttaat
     7981 tttttcagta cttcctgtgg agatttcgaa taccagtccc ttgttgtcat tcttgggatt
     8041 taatttgtgc aaaaatactt attgtctggc tttatcgaag aaacaaagaa tagcaattga
     8101 aggatgggga tggtgagtta aaagtttaaa ggttaaaagt tcgtaaagtt aaaaacctaa
     8161 aaatagggga gtggaggatg tgagtagaaa tttgaaaaag ctgataggtt tacggtgaat
     8221 cggggaaggc tttatatcac ggcaaacagt tgtgaactga ttaataaatt aaactgattg
     8281 tgtagataaa cgattttgtt ctctttgccc ttttgatttt ggctcatcac taaatatcaa
     8341 cccccaaact ctgattttag tagcgggggt aggattcgaa cctacgacct tcgggttatg
     8401 agcccgacga gctgcctctg ctccaccccg caatatattc tttattattt ctttcgtttg
     8461 attttgcaaa actaataatt ttcttcataa gaatcaaact gaactttgca agtttttttc
     8521 ttttctttga aagatttttc gaaaaatatt gtaaatcaac tatttgatat tttaagaaat
     8581 atttcggtgc ttactaaaag cttagctttc tttaaatttg tagatactct ctaacgattg
     8641 ttttttagtg agttgtatta aaatttgttc tatttgccaa ataatcaatt attttgcaaa
     8701 aaattcattt tgatgaatca taaatctggt tttgtaaata ttatcggaaa gcccaatgta
     8761 ggcaagtcga cattattgaa tgctttagtg ggagaaaaga tttctgccgt tacctataaa
     8821 gcccaaacta cacggcatcg tattttgggt atcgtaagta cagacgatta ccagattgtt
     8881 tattccgata ctcctggaat tcttgatcct gcctacaaac ttcatgaatc gatgatgaaa
     8941 tatgtagaga cctcactcga agacgccgat gtatttttat atatggtaga agcaggtgaa
     9001 agaaatcctc ccttgaatat cattcaaaga ataaaggaat caggaattcc tatcatatta
     9061 ttgatcaata agatagataa aataaagccc gagcagttgg gcgaagagat gagcttttgg
     9121 aaagaatatt ttccccaagc cgatattttg cctatttcgg cattgtatag cagtcaattg
     9181 cctgaattgg tgaataaggt cgtagagctt cttcccgaaa acccgcctta ttttcccaaa
     9241 gatgagatta ccgataggac tactcgtttt atagtttcgg aaatcatccg cgaaaaaatt
     9301 tttttgaatt atcgcaagga ggtgccatat cattctgagg tagtggtaga ttcattcaaa
     9361 gaggatgata atttcatccg tattcgtgct gtcatttatg tagccagaga atctcaaaaa
     9421 atgattatta ttggaaagaa cggcgaagcc ataaagaaga tgggcatcga agcccggaaa
     9481 gacatagaag attttgtggg gaggaaggtt tatttggaat tatcggtcaa gatagccgaa
     9541 aactggcgta atgatccacg aatgcttcgc cgcttgggct atgaattata ataaaaaacg
     9601 ctaaaccatg gcatcgaaca ttttagcaat tgttgggcgg cccaatgtag gaaaatcaac
     9661 tcttttcaac cgcctaatag gtgcaaggca agccatagtt gatccaacca caggagtaac
     9721 tcgcgaccga aactatggtc agagcgactg gaatggaatc aatttttcgg ttattgatac
     9781 cggtggctat gtggaaaatt ctgatgatat tttcgaagaa gaaattcgta aacaagccag
     9841 attggcaatc gacgaagccg atgtgattgt attccttacc aatgggcgtg atggaattac
     9901 tccattagat gaagatcttg ccaatatatt gcgtaaatcg ggcaagccag ttattttggc
     9961 cgtcaataaa atagatgatc cggtgctggt taacctggga gcagaatttt attcccttgg
    10021 catgggtgaa gtttatccta tttcagctat gacaggcagc ggtacgggag aattgctcga
    10081 tgccgtggtt gctcattttt cgtcacaacc cgaaacggaa gaaaacgaaa ttttacctcg
    10141 catagccgtt gttgggcgac caaatgttgg caaatcatca tttatcaata tgcttacggg
    10201 tacagaacgc aacattgtta cgcctattcc tggaaccact cgcgatgcca tccatactcc
    10261 atttaaggcc tttggtttcg acttcttgct cgtggataca gctggtttga gaaaaaaatc
    10321 aaaagtggaa gagaatatcg aattctattc ggtgatgcga tcagtgaggg ctatcgaaaa
    10381 cagcgatgtc tgtattttaa tggctgatgc aaatgttgga ttccaacagc aagacatgaa
    10441 catcttcagc cttatacaga gaaatcataa aggaatggtg atccttatga ataagtggga
    10501 tttggtgaat aaagacagca aaaccctgag aatttacgag gaagaagttc atagaaaaat
    10561 cgaacccttt actgatgttc ctgttttatt tacgtcggta cacgaaaaga aacgtttgct
    10621 caaggcattg gaatgggcta ttaaagttta tgaaaaccga aagcaacgca ttactacctc
    10681 ccgtctcaat gaagtgatgc tacctattat cggtcagaat cctcctccag tagtgaaagg
    10741 aaaagtggtg aaaataaaat atgctacaca attgccgaaa ccttatccgg ttttcctttt
    10801 cttctgcaat catccccagt acattcgaga agattacaag agattcatcg agaataaatt
    10861 acgtgagaat ttttctttta ctggcgttcc aatcgagatt tatttcagag aaaaatgact
    10921 gaggaagaaa ttcgtatcga taaatggcta tgggcagtac gtgtgtttaa gacacgttca
    10981 ctggcaagcc aggcttgtcg ctcaggcaag gtcagaataa atggtatggt tgtgaaacct
    11041 tcacgggata ttcgctgcgg agatgtcatt caaattagtt tgggtagctt gaataaaaca
    11101 gtcgaagtaa aggcaattct gcataatcgt atttcggcga aggaagtatc gacttacttg
    11161 atcgaccgta cacctgttga agaatatgaa cgacaaatga taattgctga aatgaagcaa
    11221 gaaattaggc ctaaaggcaa agggcggcca acaaaaaggg aaaggcgaga tatcgaccgc
    11281 ttaaaagatt ctttttaagt gctgatattg aattaaataa cttattttta ccataaaagt
    11341 atttcccgga taaatgtcga agagtgatgt aatatccttt tttggcttga aacttatttc
    11401 tttcccttga tcggcacggt tttgcgacca aacaacataa ataaacgaac ctggacgata
    11461 ttcccatctc aataccagat tcgatacaaa atacaaggct ttgaaatcag gcttgttaaa
    11521 tgaataatct ggtattccat cataatcttc atcaatttga taagttttgt ctgatggttg
    11581 ataaaaaatc tgagcttcgg aatattccgt aaatcgctgg taataatcat tagcctttgg
    11641 agaagagata aatttaaaat gcgaatattc tccgctcgaa ataaagggtt ggccataata
    11701 ttgaagtgta agatcgttgg agagattcaa gttaatacgc atttcaaggg ctaaggtttt
    11761 ttgatgaatg taagccatga tgtagcgggg tactccattc attttctttt ccttcacgta
    11821 ctgcaagttg tcgttattta ccgaatattc tggaaaaata ccgaagctca aagccgtaaa
    11881 aggttgataa tgcatcccaa ctccaaaaaa attatagtta ttattggcga taaatgttcg
    11941 ataaatgctc gcattgattt ctgctacgag ctttttccgt gagtcggttg ataaataacc
    12001 ccaataagaa tatccgccag gcaattttaa agcaggtcca cctcttagaa cgtaattatc
    12061 gaggttattg ccatcgaaat tcaatcctgc gctgatttgc caatagtttt tgaattgggc
    12121 ccatgcgttg atatttcctc ccttaaaaag gctgttcatt ccaaaatccc atccagtcca
    12181 ttgatttagg ttaaaacctg cgtttctgaa aataaggaaa ggatgatatt tctcgtatcc
    12241 cacccaagca acttggaaga tattgtcggc ctgatggaga taaccaatat cgttgatttc
    12301 cagttcaggc gaacgccagg taacaaaaag actatatttc caattgctat tgccttgacg
    12361 gctaaaatga atactgcctc catggccaga aaggcttgtt ctcgaggtat caagtttcaa
    12421 ataagtggca tcggagcgtt gaaaatttcg tataggagat tgttgcaacc gaccgataga
    12481 ttcttttgaa ccattgacta agcttgcaat ggctttgatg tctaagaaat aggtttgttt
    12541 tttccatgaa taataaaggt cgaacccacc tgttgtagcc caagttggta agagacccgg
    12601 gttgccttca ctgtggtcgc gaaatacaga agttagcatt cctcctatac ggctttcgcc
    12661 ttgatgataa tctttctgaa tgcggccgac aaaataattg ctaaaaggtt cgcatagaat
    12721 gcggttttct tcggtgcctt ccgtaatttc tgctgaagtt cggcccgtca tgctctccaa
    12781 ggctccaaac gaccatccat ccttggtttt tcccgttagt tttaaggctg ccaaaattgg
    12841 agtcgctttg ggaatgttta cataattggc atccggtgtg tattggggtt tgcgaccaat
    12901 gcgcctcgaa taaaaaagat tatcacctga aaaactatta ttgcctggcg ttatctgaaa
    12961 atccaaaata ttttttccct cgataaaaaa aggccgtttt tctgaaaaat atgtttcgaa
    13021 ggccgtcaga ttcaccacgg aagggtctgc ctcaacctgt ccaaaatcag gattgatggt
    13081 ataatcaagt attacattcg aagccaaact cactttccca tctatgccag ctctgaaaag
    13141 tgtgttctta cccgacctgt atggattccc ttcttccttt ttataagtct cgtgctgaag
    13201 tgctacataa ggagctgcct ctatctgtgg tacacgttta atatgttcta atccgtttaa
    13261 acgtccaaaa taacgtaccc atcccggagc atttcttgga atatattgcc atagcgacat
    13321 ttcttcattt cgatgaatat atctggttac ttgcaaaccc caaccctcag tattattctt
    13381 atcgaatcga agctgagaaa acgaaatctt catttcaatt gaccagcctt tatcattcac
    13441 taccgttttt gcttcccaaa ctgcatccca ggtaggatcg acatcttcca tattatcggt
    13501 ctctgattct atgccatcaa attttacgcc tgcagcattg acaaaaaaag taaaagccgt
    13561 tgtgcgatca ttataagtat ctaattgtac gcctgcccaa tccccttcta tatcgtccct
    13621 gcgtgcaagt cgcctttcga tttttttagg catactgtcg taagcctgaa taaaaaagta
    13681 tataaattca tcatcataaa ttactttgaa ttcagtagtt tggctgggtg aagtcttctc
    13741 ataaggacgc tgctgaataa aattaccaca cgcattcgtc tgcttccatt cgctttcttc
    13801 ttctattcca tccaaattaa tttttgcttc aggaaaataa actgccctgc agctttttat
    13861 tgaatcgctc aatccaaaag ctgactctga catcgtaaaa taaaacaaaa aaattgcagg
    13921 gattctaaga accgccgatt tattaaataa ccctttaagt aaatgcatga attatttgct
    13981 ggtgattgta atattcagac ggcctttttt ataaaatgtt acaaaacgaa aaatccgttc
    14041 ctgtttttag ctaagcttta accgtaaagg tgaagagatt taaaagagct gaattcaagt
    14101 cacaaatgca actttttggt tagacaaatt tagtgataa
//
LOCUS       DAOORU010000046        13933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_5359_length_13933_cov_4.774535, whole genome shotgun sequence.
ACCESSION   DAOORU010000046 DAOORU010000000
VERSION     DAOORU010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13933
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5359_length_13933_cov_4.774535"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="PLF20_04460"
     CDS             complement(<1..1190)
                     /locus_tag="PLF20_04460"
                     /inference="COORDINATES: protein motif:HMM:NF023855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HOX80294.1"
                     /translation="MSYIKFVKDQLINLEFSLAREILRSNRAGAYASQTIIGCNTRKY
                     HGLLICPQAGIDDDNHLLLSNLDESIVDEGSEFNLAIRRYKGGIYNPKGHKYAREYEI
                     DHVPIITYRIGNMVITRQLVFAMNEDRVLIKYTLVDGQSPTKIRFKPFLAFRNIHFLT
                     HANLNANTNAQRVPGGIMVKMYEGYTPLYMQFSKEAIEYLHVPDWYYDVEYLKEIERG
                     YEAHEDLFTPGYFELNLKKGENVIFSAGTTEADISKLKAIFNLEVRRRLSRSNFLNCL
                     KNAAQQFIVHRDGKTQLIAGYPYFGRFSRDTFISLPGLTLTVGNSRLCHEVLQSHLSE
                     LKGPLFPNILSKEKPVYNSIDAPLWFIWAVQKYACKTRRISSVWKEFGPAIIIANHTS
                     PVDPV"
     gene            1399..4194
                     /locus_tag="PLF20_04465"
     CDS             1399..4194
                     /locus_tag="PLF20_04465"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOX80295.1"
                     /translation="MQRFQQKLRVISILTLLLPSLILLSQKKPLTHEVYEKWNSIPYK
                     CISPNGKFVAFTIQPARGDGFLHLFNVSDHQVKVFPRGVNPAFSDNNYVIFSVKQPYD
                     TLRKLRLKKIKENDLPLDSLFILSLTTDNLLRFPHLYNFKLMEESPLVCFKRKFTPSD
                     TSSSDSTKYHPPKKKTSYQLGIFESFDKPCWFFPYIEDYATPRLNGPVAFVQKLPEND
                     SSGLYIFNPLKGQTIKVFQKPGKIKNLSWNETGTLLSFSYSTDTSFRSNICLYLYGVN
                     KNESQLIADSAILSVSTASVSPYYNPWFSQDGEKLYFAYYYPPEESKTDTLHLPEEIA
                     RLDIWSWSDPLLQSEQLKNLENEKKRSYVCMYDIKKEKIILAGRDTISQISIYMKGNS
                     RYALGSNPLPYSQLKSWDGSYYDYYLIDLQNGKAKLIERKVADYVSLSPYGYFYIRYH
                     SNDSCWYATNLKNNECIPLTKGLSACFALEEYDMPSPPPSYGIAGWATNDEFVLIYDK
                     YDIWKIDPKGKQAPENLTRFYGRKNKLKLRYLKTNPEEIHIDLEKPLLLAGYSYIDKD
                     IYILTLSPRLKREPSILTGGKCWISNLIKSRNSDTFLFTKETFQQFPDLWYAQNDFNI
                     MEQISQANPQQSEYQWGTVEVVKWISPQGDSLDGLLYKPENFDSTRQYPMIVYFYEKS
                     SQDLYSYFSPLPSRSIINPTYLVSNGYLVFVPDIVYKSDNPGNDAYDCVMSGVLSLSS
                     SGFVDKDRIGLQGHSWGGYQVAYIITRTDLFACAMAGAPVANMISAYAGIRGESGKSR
                     MIQYERSQSRIGGSLWEKPLKFINNSPLFFADNIHTPLLILHNDNDGAVPWSQGLELF
                     SAMRRLQKPCWMLVYNKEDHNLKEKSWGNRMDLTQRMYQFFDHYLKGSPAPQWMKKGI
                     PAIRKSIDNGLKFDEND"
     gene            4206..4616
                     /locus_tag="PLF20_04470"
     CDS             4206..4616
                     /locus_tag="PLF20_04470"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOX80296.1"
                     /translation="MYEHVTNLRVRYVEVDSMGYVYHGNYASYYEVGRTELMRNLGSS
                     YKKLEDEGVLMPVIHLECHYIRPARYDDEIRIVTRIQKFTHTQITFSYRIYNQDNQLI
                     NEGSTTLVFVNSQTMKPRRSPKWFAEIIEKTLTN"
     gene            5341..8286
                     /locus_tag="PLF20_04475"
     CDS             5341..8286
                     /locus_tag="PLF20_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOX80297.1"
                     /translation="MEGIEFNWDEIREQLKGDLFIEKTMRLIHATDASAYREIPLAVA
                     RPRDKSDLLTLIRFARNHRISLIPRAAGTSLAGQVVGNGIVVDMGKYMNRILEINPGE
                     KWIRIEPGVILDEMNKILKPLGLYFAPETSTSNRCMLGGMVSNNSCGAHSLIYGSTRD
                     HLLEIKAMLSDGSEVEFKDLSPAEFLAKLSGNQLENKLYRQIYHILSDPENQEEIRKE
                     YPDPSVRRRNTGYAIDSLLECQPFKPDGAPFNFCRLIAGSEGTLALITEIKLSLVPLP
                     PPVSGLVCIHLENVKDALYANLIALKYHPVSVELMDKTVLDLTHENREQDKNRFFVQG
                     NPGAILIVEFYENTLSEMEVKVEAMKTEMIQAGYGYAFPLITGKDIQRVWALRKAGLG
                     VLSNMPGDAKPVPVIEDTAVNPELLPAYIEEFDRILQRLNLSCVYYAHIGSGELHLRP
                     VLNLKDPEDVKRFREVAQQTTLLVKKYRGSLSGEHGDGRLRGEFIPLMVGERNYRHLK
                     EIKNTWDPEGIFNDRKIIDTPPMDTSLRYEPGKATKDIETIFDFSNDRGIIRAVERCN
                     GSGDCRKTEVMGGTMCPSYMATRDENASTRARANLLREMLTHSEQKNPFNHKELYEIM
                     DLCLSCKACKSECPSNVDMAKLKAEFLQHYYDANSIPFRTWAVANISTLYRLGSLIPD
                     FTNFLLANKFTSSLLKRILNFAPQRQIQTLSGVNIKKQITRYQNSKMFPKGKVYFFND
                     EFTHYTEAHIVIKAIMLLNRLGYEVIIPQHKPSGRSFISKGLLRRAKKLAISNILMLK
                     DIITEQTPLIGIEPSAILSFRDEYPDLCGQDLKEDSLRLASNCLMIDEFIDKEYKAGR
                     ITSSQFTDKPLSIKLHGHCQQKAIASTQATISMLSIPKNYKVEEIPSGCCGMAGSFGF
                     EKEHYALSQKVGELVLFPEIRKTPENVKIAAVGTSCRNQILEGTGRQALHPLEILWES
                     LISDFEIKT"
     gene            complement(8586..9182)
                     /locus_tag="PLF20_04480"
     CDS             complement(8586..9182)
                     /locus_tag="PLF20_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOX80298.1"
                     /translation="MDEDAFLTIENSGEGYFKSKGSRFISFAIPVKDEEEIDFHLKAI
                     KRQYADATHHCFGWSLGRQRERYRYYDDGEPSGTAGRPIYGQILSAGITNVLIVVVRY
                     YGGTKLGTSGLIEAYKTAASEAIGKVAKITGYEIKSLYLEFDYSSMEVVMRKIKQYKF
                     QIAESEYGEKCFIHLKVRKSFYDSAVEIFSDVNGIVVR"
     gene            9340..9639
                     /locus_tag="PLF20_04485"
     CDS             9340..9639
                     /locus_tag="PLF20_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80299.1"
                     /translation="MIQFIKNLYNFAELILKPKTAMKKLNLFLLAGAVALIAACGPSA
                     KEKAEKARQDSIRIADSIAKVKAYEDSIAAAQAAAEQARLDSIAQAEEAQKPKKK"
     gene            9754..11775
                     /gene="uvrB"
                     /locus_tag="PLF20_04490"
     CDS             9754..11775
                     /gene="uvrB"
                     /locus_tag="PLF20_04490"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOX80300.1"
                     /translation="MQFKLVSEYQPTGDQPEAIRQLVEGIERGDKAQVLLGVTGSGKT
                     FTIANVITQVNRPTLVLSHNKTLAAQLYGEFKQFFPENAVEYFVSYYDYYQPEAYLPV
                     TDTYIEKDLSINEEIEKLRLSTSSSLLSGRRDIIVVSSVSCIYGIGNPDDFNRQVIHL
                     QKGQIVGRSKILLDLVAGLYSRTVNDFKRGTFRVKGDVVDIFPAYADKAYRIMFWGDQ
                     IEELQIFDPLTGYKISDEDNLLIYPANIFVTSHEQMVKAIHNIQDDLFKQVEYFNQIG
                     KFQEAKRLEDRVTYDIEMMKELGYCSGIENYSRYFDGRKPGSRPFCLIDYFPDDYLLI
                     IDESHVTVPQIRGMYGGDRSRKQVLVDYGFRLPSALDNRPLKFDEFEKLTNQIIYVSA
                     TPAEYELKQAEGIVVEQFIRPTGLLDPKIEVRPSTNQIDDLLSEIQKNVERNERVLVT
                     TLTKRMAEELSKYLQSINVKTRYIHSDIETLNRVEIMRDLRLGKFDVLVGVNLLREGL
                     DLPEVSLVAILDADKEGFLRSERSLTQTAGRAARNINSRVIMYADKITDSMRLTIEET
                     NRRRSMQTEYNQMHGITPTPIIKSTSEILGQTSVVDVKKPQSNYYVEKEEIEVAADPL
                     VKYLNKEQIQKIIQQTRKNMEAAVKELDFINAARFRDEINQLKQLLETK"
     gene            complement(11971..12453)
                     /locus_tag="PLF20_04495"
     CDS             complement(11971..12453)
                     /locus_tag="PLF20_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOX80301.1"
                     /translation="MQIAQNSVVSLIYTLQANNSEGEILQIATQENPLTFIYGHGHLL
                     PSFEENIRGLEPGSSYEFALSYEEAYGPFDPESVLDVDIEIFHQTPEGEQMLFIDNVI
                     PLMDQDGNRFEGRVIQIDENSVKMDFNHPLAGMTLFFTGSILDVRPASPEELAHGHVH
                     "
     gene            complement(12546..13736)
                     /gene="sucC"
                     /locus_tag="PLF20_04500"
     CDS             complement(12546..13736)
                     /gene="sucC"
                     /locus_tag="PLF20_04500"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF001913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOX80302.1"
                     /translation="MNLHEFQGKSIFKSYGVNVPEGILAFNAKEAVEAAQILEEKTGT
                     NCWAIKAQIHAGGRGKSGGVKIAKSLKEVETFAAQIIGMKLVTPQTSAEGKLVRKVLV
                     EQNIYYPGEEPIQEFYLSILLNRHRGRTIIMYSPRGGMDIEAVAEETPEQIFTEEIDP
                     RVGLQNFQCRRIAFNLGLSGEAYKQMLQFVDALYRAYQASDASLFEINPVIKASDGKI
                     FAADAKVIIDNNALFRHPDILQMRDLDEEDPIEVEAGNYKLNYIKLDGNVGCMVNGAG
                     LAMATMDMIKMSGGAPANFLDIGGTADAKRVEQAFRIILKDPNVKAILINIFGGIVRC
                     DRVAQGIIDAFNNIGQIDVPVIIRLQGTNAREASELIQNSGLKVLTAIELQEAAELVN
                     KILS"
BASE COUNT         4381 a         2803 c         2683 g         4066 t
ORIGIN      
        1 accggatcaa cgggacttgt gtggtttgcg atgatgatgg ccgggccaaa ttccttccat
       61 accgatgaaa tcctccgagt tttgcaggca tacttctgta ctgcccaaat aaaccataaa
      121 ggagcgtcga tggagttata cactggtttt tctttgctca agatatttgg gaaaagagga
      181 cccttgagct ctgacaaatg agattgcagc acttcgtggc ataatctgga attgccgacc
      241 gtcaatgtaa gaccaggaag tgaaatgaag gtatctcgac taaatcttcc aaaataagga
      301 taacctgcta ttaactgagt tttgccgtca cggtgaacaa taaactgctg agctgcattt
      361 ttcaggcaat tcaaaaaatt actgcgcgac aagcgtctgc gaacctccag gttaaaaatg
      421 gctttcagct tcgaaatgtc ggcttcggtc gtccctgccg aaaatattac gttttctcct
      481 ttttttaggt tcaattcgaa atagcctggg gtgaaaagat cttcgtgcgc ttcatagcct
      541 ctttcgatct cttttaaata ttccacatcg taataccaat caggaacatg aagatattca
      601 atcgcctctt tcgagaattg catatataaa ggtgtatagc cttcgtacat ctttaccatt
      661 attcctccgg gcactctttg agcgttagta ttggcattta aattggcatg ggttagaaaa
      721 tgaatattcc gaaaggctaa aaagggttta aaacgaatct tcgtaggcga ctgcccatca
      781 acaagcgtat atttgatcaa aaccctgtcc tcattcatgg caaatacgag ttgcctcgta
      841 atgaccatat tgcctattcg ataggtaatt atgggtacat ggtcaatttc atattctcgt
      901 gcatatttat ggcctttggg attgtatatt cctcccttgt atcttcttat cgccagattg
      961 aattcgcttc cttcatcgac gatactttca tcgagattcg aaagcaacag atgattatca
     1021 tcgtctatgc cagcttgtgg acaaatgagc agaccatgat atttacgcgt attacaccct
     1081 atgatggttt gagaagcata agcaccggca cgattcgatc gtaaaatctc gcgtgcaagt
     1141 gaaaactcaa ggttaataag ttgatcttta acaaatttaa tataactcat acaatgaatt
     1201 tctaaaagtc ataggccatt ttgagaatgc aaataaacat atttcaaatg aaatataaat
     1261 taactttcga aaacaattct aacagcaaat atgctaaaat ttccttttgc ttccatgcat
     1321 tcttcgtaat tttacacctt ctttctacaa tttctgcggt ttgtttaaat ttataaaagt
     1381 attaaaaaaa tccctgaaat gcaacgtttt caacagaaat tgagggttat atcgatttta
     1441 actctcctct tgccttccct catcctctta tcccagaaaa aaccacttac tcatgaggtt
     1501 tatgaaaaat ggaactccat tccgtataaa tgcataagcc cgaatggaaa gtttgtagct
     1561 tttaccatac agcctgcaag aggtgatggc ttccttcatt tgttcaatgt ttccgaccat
     1621 caggtaaaag tttttcccag aggggttaat cccgcatttt ctgacaacaa ttacgtgata
     1681 tttagcgtta aacagcctta tgatactttg agaaaattgc gtttgaaaaa gataaaagaa
     1741 aatgacttac ctctcgattc cctcttcatt ctttcactta ccactgataa cctgctcagg
     1801 tttccccatc tctataattt taaattgatg gaagaaagcc ctttggtttg tttcaaaaga
     1861 aaatttaccc cctcagatac aagttcttcc gatagcacca aatatcatcc tcctaagaaa
     1921 aaaacatctt atcaactcgg tattttcgaa tcattcgata aaccatgctg gtttttccct
     1981 tatattgaag attatgctac accccgatta aacgggcctg tggcctttgt tcaaaagcta
     2041 cccgagaatg attcttctgg cctttatatt tttaatcctt taaaaggcca gacaatcaaa
     2101 gtttttcaaa agcccggaaa gatcaaaaat ctaagttgga acgagacagg aacattgctt
     2161 agtttctcat attcaacaga cacttctttt cgctcgaata tttgcttata cttgtatgga
     2221 gtaaataaaa atgagagtca acttatagca gattctgcaa ttttgtcagt ttctactgcc
     2281 agcgttagtc catattacaa tccctggttt tctcaagatg gagaaaaatt gtatttcgca
     2341 tattactatc caccagaaga gtctaaaaca gacacgcttc atcttcccga agaaattgca
     2401 aggttggaca tctggagctg gagtgatccc ttgctgcaat cagaacaact caaaaatctc
     2461 gaaaatgaaa agaagcgctc ctatgtttgc atgtatgata tcaaaaaaga aaagataatt
     2521 ttagcaggaa gggacaccat ctctcaaatt tccatttata tgaagggaaa ctcgagatac
     2581 gctctgggaa gcaatcccct cccctattct cagctgaaat cgtgggatgg cagctattat
     2641 gactactatt taatagattt acagaatggc aaggcaaaac tcatcgaaag aaaggttgcc
     2701 gattatgtca gcctttcgcc ttatggctac ttttatattc gatatcattc gaatgattca
     2761 tgctggtatg ccacaaattt aaagaacaac gaatgcatcc cgcttaccaa agggttgtca
     2821 gcatgtttcg ctctcgaaga atacgacatg ccttcccctc ctccctccta tggtattgca
     2881 ggatgggcaa ctaatgacga atttgtgttg atttacgata aatatgatat atggaaaata
     2941 gatcctaagg ggaaacaagc tcccgaaaat ctcactcgct tctatggaag aaaaaataaa
     3001 ttaaaattga ggtatttgaa aaccaaccca gaagaaattc atattgacct cgaaaagcct
     3061 ctattgcttg ccgggtatag ctatatcgat aaagacatat acattttaac actttctcct
     3121 cgattaaaac gtgaaccttc catattaaca ggaggcaaat gctggataag caatttgata
     3181 aaatcaagga attcagacac ttttctattt acaaaggaaa catttcaaca attccccgat
     3241 ctatggtatg cccaaaacga ctttaatatt atggagcaga tttcacaagc caatccacag
     3301 caatcagaat atcaatgggg aactgtggaa gtggtgaaat ggatatcacc tcaaggtgat
     3361 tctttagatg gtttactcta taaacctgaa aatttcgatt cgacaaggca atatccaatg
     3421 attgtctatt tctatgagaa gagttcgcaa gatctataca gctatttttc acctttacca
     3481 tcgcggtcta ttattaatcc tacttatctc gttagcaatg gatatctggt atttgtgccc
     3541 gatatagtat ataagagcga caatccggga aatgatgctt acgactgtgt aatgagcgga
     3601 gttctatcct tatcgtcctc aggctttgtg gataaggatc gtattggatt acagggtcat
     3661 agctggggtg gatatcaagt ggcttatatt attacacgca ccgacctatt cgcctgtgcc
     3721 atggctggtg caccagttgc caatatgatt agtgcttacg ctggaatacg aggggaatcg
     3781 ggaaaaagtc ggatgataca atacgaaaga tcacaaagca ggattggagg tagcctttgg
     3841 gagaaacctt tgaaatttat caataattct cctttatttt ttgccgataa cattcatact
     3901 ccccttctca tcttgcataa cgataatgat ggagctgtac cctggtcgca ggggctggag
     3961 ttatttagcg ctatgaggcg tttacagaaa ccatgctgga tgcttgtata caataaagaa
     4021 gaccacaacc tgaaggaaaa atcatgggga aaccgcatgg atttgactca acgaatgtac
     4081 cagtttttcg atcattattt aaagggaagt cctgctcctc agtggatgaa gaaaggcatt
     4141 ccagctattc gaaaaagtat cgataatgga ttgaaattcg atgaaaatga ttaatactcg
     4201 ctattatgta tgaacacgtc accaacctta gagttagata tgtcgaagta gattcgatgg
     4261 gttatgttta tcatgggaat tatgcctcct attatgaagt cggacgcacc gaacttatgc
     4321 gcaaccttgg aagttcctat aaaaaattgg aagatgaagg agtattgatg cctgttatcc
     4381 atctcgaatg ccattacata aggccagcta ggtatgacga tgaaattaga attgttaccc
     4441 gaattcaaaa atttacacat actcaaatca ctttctctta tagaatctat aaccaagaca
     4501 atcaattgat caatgaaggg tcaacaaccc tcgtatttgt caatagccaa accatgaaac
     4561 ctcgaaggtc acccaagtgg tttgccgaaa taatcgaaaa aactctgacc aattgaaaat
     4621 aatgcttcgc attttataaa atacaaaatt cgaaatgcca cggaactttt cgacagattg
     4681 tatatgatgg gaatcactca aagccacagc gcaaagaaaa tgaatgattt atccgttcaa
     4741 tttggagaaa gcatagaata taatcacaat gcttcctaca ctttcaatca ctctgaattg
     4801 gacaggcggt ctggcaatac cttttatcga gatgaattat tcaaccaaag accattaatt
     4861 attttagcga tggtcattaa tccttttcac gataaaaaaa tttctagccg aaaattttag
     4921 agtagaagaa aactcttagt cgtccacaat cttcttctct cttattgtaa attttgtgaa
     4981 taaattcccc gcgtctcatt ggggaatgta ttcctgaaga gcaaggattt ttgtcggctt
     5041 tgggagtaga aaaatcaagg caaatctccg aacattcact ggcacaatct aatagcatgg
     5101 taactttaag caatgttgac tttatggaat accaatgcac caaagttttt ccttgttgcg
     5161 aatttgcaaa atcttatttc tcttctggag agcagttata cgatagggtt atcaaaaatc
     5221 ggccgatagg atattcgaaa gaactgccaa atccactatt cgaaaaataa ttcgtcgaaa
     5281 gattctacag agggtaaagc ccgctttttt tatttttgcc atccatataa aaaaaataaa
     5341 atggaaggaa ttgaattcaa ttgggatgaa atccgtgaac aactcaaggg agatcttttt
     5401 atagaaaaaa cgatgcggct gatacatgca acagatgcat cggcttaccg ggaaattcct
     5461 ttagctgtag caagaccacg cgataagtct gacttattaa ccttgattcg ttttgcccgt
     5521 aatcatcgta tttctttaat accaagagct gcagggactt ctctggcagg ccaggtagta
     5581 gggaatggca ttgtggtaga tatgggaaaa tatatgaatc gtatactcga gatcaacccc
     5641 ggcgaaaaat ggatacgaat agagcccggc gtgatactcg atgagatgaa caaaatattg
     5701 aagcctttag gattatattt tgctccagaa acctcaactt ctaatcgttg catgcttgga
     5761 ggcatggtga gtaacaattc atgtggtgct cattcattaa tctatggcag cacaagagac
     5821 catttgctgg agataaaggc tatgttgagc gatggtagcg aagtggaatt caaagatctt
     5881 tctcctgcgg aattcctggc caaactaagt ggcaatcagc tcgaaaataa attatatcgt
     5941 caaatttatc atattttatc cgatcctgaa aaccaggaag aaatccgaaa agaatatccc
     6001 gatcctagtg ttcgtcgtcg aaatacaggt tatgccatcg actctttgct cgagtgtcaa
     6061 ccatttaaac ccgatggtgc tccattcaac ttctgccgtt taatagcagg ctcggaagga
     6121 acacttgctc taataacgga aattaagcta agtttggttc ccttgccgcc tccagtttca
     6181 gggcttgttt gcattcattt agaaaatgta aaagacgcat tatatgcaaa tctcattgca
     6241 ctgaaatatc atcccgtttc ggttgaacta atggataaaa cggttttgga tttaacccat
     6301 gaaaaccgtg agcaggacaa gaatcgtttt tttgtgcaag gtaatcctgg ggctattcta
     6361 attgtagaat tttatgaaaa tactctctcg gagatggaag taaaagttga ggccatgaaa
     6421 acggagatga tacaggctgg gtatggttat gcttttccac tcatcacggg aaaggatatt
     6481 caacgcgttt gggctttgcg aaaagccggg ctgggagttt tatcgaatat gcccggcgat
     6541 gctaaacccg ttcctgtcat agaagatacc gcagtaaatc ccgaattact tccagcttat
     6601 atcgaggaat tcgatcgaat cctccagcgt ttgaatttat cctgtgttta ttatgcacat
     6661 atcggcagtg gtgaactaca tctaagacct gttttgaacc taaaagaccc ggaagatgtg
     6721 aaacgctttc gagaagtggc tcagcaaaca actttgctag taaaaaaata ccgaggttca
     6781 ttgagtggag aacatggtga cggacggtta cggggtgaat tcattcctct aatggttggg
     6841 gaacgtaatt acagacattt aaaagaaatt aaaaacacat gggatcctga aggaatattc
     6901 aatgatagaa agatcatcga tactcctcct atggatactt cgcttcgtta cgaaccaggg
     6961 aaggcaacca aagatatcga aaccatattc gatttttcga acgatcgagg aattatcagg
     7021 gcagttgaga gatgcaacgg ttcgggcgat tgccgaaaga cagaagtgat gggaggaacc
     7081 atgtgcccaa gttatatggc tactcgcgat gaaaatgcaa gcactcgcgc ccgagcaaat
     7141 ttactgcgtg aaatgcttac ccattccgaa cagaaaaatc cattcaacca caaagaactc
     7201 tatgagataa tggatttatg cttatcgtgc aaggcatgca aatcagaatg cccatcgaat
     7261 gtagacatgg caaaattaaa agccgaattt cttcagcatt attacgacgc aaacagcatt
     7321 cccttcagaa catgggctgt agccaatatt tcgactcttt atcgattggg tagcctgatt
     7381 cctgatttca ctaacttctt acttgccaat aaatttacct cctctctttt gaagagaatc
     7441 ctgaattttg cccctcaaag acaaatacaa acactcagtg gtgtaaatat caagaagcaa
     7501 ataactcgtt atcagaattc aaagatgttt cccaagggta aggtctattt ttttaacgat
     7561 gaatttaccc attataccga agcccatatc gttataaaag ccatcatgtt actgaatcgt
     7621 ctgggctatg aagtgattat cccacagcat aaaccgagcg gccgttcctt tatttcgaaa
     7681 ggactcctcc gcagagcaaa aaaattagct attagtaaca ttttgatgct taaagatata
     7741 attactgaac aaacacctct tattggaata gaaccttcag ctattctttc gttccgggat
     7801 gaataccccg atctatgtgg gcaggattta aaggaagata gcttacggct ggcttctaat
     7861 tgcttgatga ttgacgaatt tatcgacaaa gaatataaag ctggcagaat tacatcatct
     7921 caatttactg ataaaccgct ttcgataaaa ctccatgggc attgccaaca aaaggccata
     7981 gcttcaacac aggctactat ttccatgtta tctatcccca aaaactacaa agtagaggaa
     8041 atcccttcgg gctgttgtgg aatggccggc tcattcggat ttgaaaaaga acattatgcc
     8101 ttgagccaaa aagttggcga attggtcctg ttccccgaaa tacgaaaaac acccgaaaat
     8161 gtcaagattg ctgctgtagg aacatcgtgc agaaatcaaa tccttgaagg tacaggcagg
     8221 caagccttac atccgttgga aatcttatgg gagagtttga tctcggattt tgaaataaaa
     8281 acctgattgc caatgccgca agtgccattg caaactatat tgatacggcc aatggtgtgc
     8341 gggcatagaa tgttactcgg cctctaactt taaaaattga agttcggatt gttggaattt
     8401 gcgagaaaga aaaattcaag tcagcacaaa ccatatttat tacgatgatt acttgatttt
     8461 tatggcaaaa ggaatcgaaa gtgatctcaa ggttaataaa caaaaacaac cttatgaaca
     8521 tcttcgagag gtattcaacc tgtcagtagc ctttattgtt tagatggatt aataagcgag
     8581 cttgcttatc tgacaactat accattcaca tcactaaata tttcaacagc actgtcataa
     8641 aacgattttc gaaccttcaa atgaataaag catttttcac catattcgga ttcggcaatt
     8701 tgaaacttat actgctttat ctttcgcatc acaacctcca ttgaagaata atcaaattcc
     8761 agataaagac tctttatttc atatcctgta attttggcaa ccttgcctat ggcttctgag
     8821 gctgctgttt tatatgcttc tatcaaaccc gaagttccta atttcgttcc accataatat
     8881 cttaccacca caataagaac atttgtgatt cctgcagata aaatctgacc gtaaataggt
     8941 ctgccagccg ttcccgaagg ttcaccatca tcataatagc ggtacctctc ccgttgcctg
     9001 cccaaactcc acccaaaaca gtgatgagta gcatcagcat attgccgttt gattgcttta
     9061 agatgaaagt cgatctcttc ttcatccttc acgggaatgg caaaagaaat aaaacgactt
     9121 cccttgcttt tgaaatatcc ttcacctgaa ttttcaatgg ttagaaatgc atcttcatcc
     9181 ataggggcaa atttacaatc aagatgcaaa tccggcaacg tctatggaat ttttaagcat
     9241 aatacattga tatatactta tttaagccct aaatgaatca ttaagaaatc ttaaattgtt
     9301 cgtaaatttc ttaaagaatg ttaaattttt ttttgaaaaa tgatacaatt tattaaaaac
     9361 ctttataatt ttgcagagtt aattttaaaa ccaaaaaccg ctatgaaaaa attaaatctt
     9421 ttccttttag caggtgcagt tgctctgatt gccgcttgcg gaccaagtgc aaaagaaaaa
     9481 gctgaaaaag ctcgtcagga ttccattcgt attgccgact caattgctaa agtaaaggct
     9541 tacgaagaca gtattgctgc tgctcaagcc gctgctgaac aagctcgtct ggatagtatc
     9601 gctcaagctg aagaagctca gaaaccgaag aaaaagtaag gaaacttctg attgtcagaa
     9661 ataaaaaagg gagtgtatat tcgtacattc ccttttttat attcacctaa agaggtatta
     9721 aatttgatgg cagtttcaga attcaccaat gttatgcaat tcaaactggt ttcggaatat
     9781 caacccacgg gtgatcaacc cgaagcaata cgccagcttg tcgaaggaat agaaagaggc
     9841 gacaaggctc aggtattact cggtgttacg ggttcaggaa aaacatttac catcgctaat
     9901 gtcattactc aagtaaatcg tcctactctt gtgctgagcc ataataaaac ccttgcagct
     9961 cagctctacg gagaatttaa acagttcttc cccgaaaacg ccgtcgaata ttttgtttca
    10021 tattatgact attatcagcc cgaagcttac ttacctgtta cagacaccta catagaaaaa
    10081 gacctttcca tcaatgaaga aatcgaaaaa cttagactaa gtacgagttc atccctttta
    10141 tctggtcgaa gagatattat tgtagtttca tcggtttctt gcatttatgg tataggaaat
    10201 cccgatgatt tcaaccgcca ggtcattcac ttacagaaag gacaaatcgt cggccgtagt
    10261 aaaatactct tagatttggt agctgggctc tattcgcgta ccgtaaacga cttcaaaaga
    10321 gggacgttca gagtgaaagg agatgtggtc gatatttttc ctgcttatgc cgacaaagct
    10381 tacagaatca tgttctgggg tgatcagatt gaagaacttc aaattttcga tccacttacg
    10441 ggatataaaa tctcggacga agataatttg ctgatttatc ccgcaaatat ctttgttact
    10501 tcgcacgaac aaatggtaaa agcgattcat aacattcagg acgatttgtt caaacaagtg
    10561 gagtatttta atcaaattgg aaagtttcag gaagctaaac gactcgaaga cagagttacg
    10621 tacgatattg agatgatgaa agaattaggt tactgctcgg ggatagaaaa ttattcgcgt
    10681 tatttcgatg gtcgtaaacc tggctcgagg ccattctgcc tcatagatta ttttccggat
    10741 gattatctct tgatcattga cgaaagccat gttacggttc cgcagattcg tggaatgtat
    10801 ggaggtgatc gttcacgcaa gcaggttctg gtagattatg ggtttcggct cccttctgcc
    10861 ctcgataacc gacctctgaa attcgatgag ttcgaaaaac taaccaatca aataatttat
    10921 gtgagtgcta ctccagcgga gtatgaatta aaacaggccg aaggaatagt ggtggaacaa
    10981 ttcatcagac caaccggact tctcgaccct aaaatagaag tcagaccttc aaccaaccag
    11041 atcgatgact tattgtcgga aattcaaaaa aatgtggaaa gaaatgaaag agttctggtt
    11101 acaaccttaa ctaaaagaat ggcagaggaa ctcagtaaat atttgcaaag tatcaatgtt
    11161 aaaactcgct acattcattc tgatatcgag accctcaaca gggtcgaaat catgcgtgat
    11221 ttacgacttg gaaaattcga tgtattggtg ggagtaaatc ttctaagaga agggctcgac
    11281 ctgcctgaag tttcattggt agctatcctg gatgctgaca aggaaggttt cttgcgctcg
    11341 gaacgatcgt taacgcaaac agccggacgt gccgcccgaa atatcaacag ccgggtcatt
    11401 atgtatgctg ataagattac cgattctatg cggttgacta tcgaggaaac aaatagaagg
    11461 cgatcgatgc aaacggaata caaccagatg cacggcatca ctcctacccc catcatcaaa
    11521 tcgacgagtg aaattcttgg gcaaacctcg gtggtagatg tgaaaaagcc tcagtcaaat
    11581 tattatgttg aaaaagaaga gattgaagtc gctgccgacc ctcttgtgaa atatctaaac
    11641 aaagagcaga tacaaaaaat cattcagcaa acccgaaaaa atatggaagc agccgtaaaa
    11701 gaactcgatt ttatcaacgc agcccgtttt cgagacgaaa ttaaccagct taaacaactt
    11761 ctcgaaacta aataacacat ttttataagt tcttatcaac aaatattgtt gattctattt
    11821 tgtaatattt tgtataatag taagttataa aattagctcc aactattgaa aatttgccaa
    11881 atataaaatc aagggggttg aatcttcagg cagtccgaaa aaatttaatt cagatgaatt
    11941 attatggaat caacagccgt ggttgccttc tcaatgaaca tgtccatggg ccagttcttc
    12001 gggagaggct ggtcttacat cgagaatgga acccgtaaaa aataaggtca ttccggccag
    12061 aggatggtta aaatccattt ttaccgaatt ttcatcaatt tgtattactc ttccttcgaa
    12121 tcggttacca tcttgatcca tcaaaggaat gacgttatct ataaaaagca tttgttcacc
    12181 ttcgggcgtt tgatgaaaaa tctcgatatc gacatcgagt actgattccg ggtcgaaagg
    12241 gccataggcc tcttcatacg acaaggcgaa ttcataggaa gatccgggtt ccaaaccacg
    12301 aatgttttcc tcgaaggaag gaagcaaatg tccatgcccg taaatgaaag tcaatggatt
    12361 ttcttgggtg gcaatttgaa ggatttctcc ttccgagtta ttagcttgaa gtgtataaat
    12421 gaggctaaca acggagtttt gtgcaatttg cataaaataa aaataaagtt aatttaaaaa
    12481 attgaatatg attcgactaa aacattttgt ctttatgaaa agcgagcaaa agtataaaat
    12541 ttatttcagc taagaatttt attcaccagt tcagcggctt cttgaagttc gatagccgtc
    12601 aacactttga gtccgctatt ttggattaat tcggatgctt ctctggcatt tgttccttgc
    12661 agacgtataa tgacaggcac atcgatttgt ccaatattat tgaaagcatc tatgatacct
    12721 tgagctaccc tgtcgcatcg aacaatccct ccaaagatat taataagtat ggctttcacg
    12781 ttagggtctt tcaaaatgat gcgaaaggct tgttcaacgc gtttagcgtc ggctgtgcct
    12841 cctatatcga gaaagttggc aggcgctccg cccgacatct tgatcatgtc catggtagcc
    12901 atggccaacc ctgctccatt gaccatacag ccaacattgc catcgagttt gatgtaattg
    12961 agtttatagt ttcctgcttc cacttcgatg gggtcttcct cgtcgaggtc tcgcatttgc
    13021 aagatatcgg gatggcgaaa aagcgcatta ttatcgatga taactttggc atcggctgca
    13081 aagatttttc cgtcggaggc cttaattaca ggatttatct cgaacagaga agcatcgctt
    13141 gcttggtaag cccgataaag tgcatcgaca aattgtaaca tctgtttata ggcttcgccg
    13201 gataatccaa gattgaacgc tatgcgcctg cattgaaaat tttgaaggcc tactctggga
    13261 tctatctctt cggtgaaaat ttgttcgggc gtttcttcgg ctacggcttc gatatccatt
    13321 ccacctcttg gagaatacat aattatggtg cgaccacggt ggcgattcag caatatgctt
    13381 aggtaaaatt cctgtatggg ctcttcacca ggataataaa tattctgctc caccaacact
    13441 ttccttacca gcttgccttc ggcagaagtt tgaggtgtaa cgagcttcat accaataatc
    13501 tgagcagcaa aagtttcgac ttccttgagc gatttggcaa tctttacacc tccacttttc
    13561 cctctcccac ccgcatgaat ctgagcctta attgcccagc aattagtacc cgttttttcc
    13621 tcgagaattt gcgctgcctc aactgcttct ttcgcattga aagctaaaat gccttcagga
    13681 acatttactc catagctttt aaaaatgctt ttcccctgaa attcatgaag attcataaag
    13741 aaaattcgat tttttagagc attcaaaggt atgtattaat ccaattctca atcgttaatg
    13801 cgtttcactt attcacacct ttgataaaat atttagagaa aaatcaacgt tctttgcaaa
    13861 atcatcagct tatggatgga ttatgaaaaa aatattcgcc ttcctctttt tgaccttagc
    13921 cttcattacc tcc
//
LOCUS       DAOORU010000047        13885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_5391_length_13885_cov_5.900940, whole genome shotgun sequence.
ACCESSION   DAOORU010000047 DAOORU010000000
VERSION     DAOORU010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13885
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5391_length_13885_cov_5.900940"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1612)
                     /locus_tag="PLF20_04505"
     CDS             complement(<1..1612)
                     /locus_tag="PLF20_04505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80303.1"
                     /translation="MKKIFFTLIASLFIIITSDLPLQAQNWKIVVGHPYKGWGNDYAY
                     NIKIIDNSPYIVGSSSSKNLGTTPNGGRDAWLLKTDYLGNILSTQGFGGSGFEYFNNV
                     FPAPDSGLYLLGSTISSDDIFSFNPYLGGISAFALKLDSSNNIVWNHIYGGNRTDELK
                     DAVMTYDGGFVFVVWSTSNDGDVGQNFGAVDVWVVKLTDEGQILWSKVFGNHFIDIVS
                     TIIETSDKGLLIGGSFDYYKPGLGNLFCDTCYGNAEAFLIKLDSVGNVCWTKCYGGPG
                     YDGFSSLLEVSDGYVLGGYASAGGGLVTGFHNNAMGYNDAWVIKTDFEGNIIWTKCLG
                     GSGTEIVYKMFKEKDGNLMIFSMTDSHDGDVNSNFSDYYYMWLVLLNGQDGSIIKEKC
                     INVVGTYWGAAAQIEYGDYILLINVPTLYNWVDVWFYRIKDCNEEQIPPAPAEPKGPQ
                     QINTYTTTTSFYSLTPDGIALSYTWELNPPEAGWLMTPADTTIEVVWNPNFWGTARLK
                     IRGTYLCGIGPWSSELKISVNLVGMEEPD"
     gene            2267..2950
                     /locus_tag="PLF20_04510"
     CDS             2267..2950
                     /locus_tag="PLF20_04510"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HOX80304.1"
                     /translation="MKKIFLFIALILSLEQAVAQKHFYFELRAFDKIQINGNIDAQIS
                     RAGEEDATLSVNGIEPDRVSIEVEGRTLKIKIGFFEGRDALVKVWINYKEIREVNASN
                     GASLHFTEPIKSNYMKISAGTGSSIDAQGEFRILEIKASAGSKINIRGKADIADISAN
                     TTAEVQAEGLVCQEGILKAYTGAKITIQIEQSLEATSATGAEITVKGNPVIEKVSTST
                     GGILTRIKL"
     gene            complement(3812..4255)
                     /locus_tag="PLF20_04515"
     CDS             complement(3812..4255)
                     /locus_tag="PLF20_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80305.1"
                     /translation="MNIQTNKSDDLLREVFSKIPSESPSEDFTQKVMDQIARENVEKL
                     SINANFNLGFRTLLALALTIGFFGILIFTSDFSFMDYIYQIFSTLKFKLNPNQLFHPF
                     VFLSKTNNLSLIVGLVMLAGGALLVFSHNTNKKDKSPGMDSFLSL"
     gene            complement(4252..4845)
                     /locus_tag="PLF20_04520"
     CDS             complement(4252..4845)
                     /locus_tag="PLF20_04520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX80306.1"
                     /translation="MAEINDEILIQRTLRGDQFAFAQIVEKYKQTAFSIAYQITGNRE
                     DAEEIAMDAFVKAYQALKGFKFESKFSTWLYRIVYNTAISKKRKKKMEFVAIDHHLIE
                     NYSEDNFVAGLQMLDTDEQLKLVQHVLKSLPGDDAALINLFYNQQLNIEEISHITSLS
                     VSNVKVKLHRIRKRIFAGVQLLLNQSTSTYEYKNSKS"
     gene            5006..5341
                     /locus_tag="PLF20_04525"
     CDS             5006..5341
                     /locus_tag="PLF20_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80307.1"
                     /translation="MNGEILIPLGFFAMVVAIVYLALRQRERMKMINQGMDPAASNTG
                     LESTLQLRLGLIFIGIAVGFLLGDIFSSLHWLKEWVAYISMVTIFTGIALIVSYYLIN
                     KIPPRDRKE"
     gene            complement(5357..5935)
                     /gene="thpR"
                     /locus_tag="PLF20_04530"
     CDS             complement(5357..5935)
                     /gene="thpR"
                     /locus_tag="PLF20_04530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOX80308.1"
                     /translation="MTLKRIFIAVDYQPDSDFLYYFSFLRRQLRNEDITWSATQNFHI
                     TLKFFGDTPEDHISSIIHQLDGITKKYESFQIQPNKLGIFGSLKQARALWLSINESTG
                     TLESLAQEIEHAFLTDPSKKNNSFTPHLTLGRPKFIRQPDLLKNLMESSSQHVFNPFA
                     IREVLLIWSTLTPLGPQYKTLKSFPLQPLTLE"
     gene            complement(5962..6423)
                     /locus_tag="PLF20_04535"
     CDS             complement(5962..6423)
                     /locus_tag="PLF20_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80309.1"
                     /translation="MESDDNFEDKIEKLIRLFKKLKEQAKPETIAGFDPFMMSQLDFL
                     ISNFERMKYDPQARSIFQQMGKPFENMLNLFIKNLSEQLGEPVNIPKKSERETLLRPL
                     PEEEKPSIGTLTRRLVQIDKLLAEGNLNQEEEDQLLDERIKIMKKLNPEDE"
     gene            complement(6427..7800)
                     /locus_tag="PLF20_04540"
     CDS             complement(6427..7800)
                     /locus_tag="PLF20_04540"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HOX80310.1"
                     /translation="MEDITTYLEKNSNRIMEELFGLLKIPSISSLSEHKDDMYRAAEY
                     YKNALLKAGMDKVEIFDTPGHPVVYAEKILSPDFPTVLVYAHADVMPVDPIELWQSPP
                     FEPEIREGKIYARGADDDKGQGFMHVKAIEYLVNSRQLQCNVKFMIEGEEEIGSPHLE
                     DFCQEHKDMLKADIILLSDTSMIAPDTPSITVGLRGLAYIEVEVAGPNRDLHSGLYGG
                     IVHNPIHVLSRMIGNLHDDHGRIIIPGFYDDVRELSEEERIEFNLAPFDEESYKKSLG
                     INCLWGESGFTPLERIGIRPSLDVCGIWGGYTGEGSKTILPSKASAKVSMRLVPDQDP
                     KKIANLFENYLHEMVPNTVKLKVTALHGGYPYLAPTNIPAYKAAQKAYLKTFGKYPIP
                     TCSGGSIPIISTFDRVLGIKSLLLGFGLESDAIHSPNENFSVKNFFKGIETISEFYIQ
                     FAHITKD"
     gene            complement(7943..8731)
                     /locus_tag="PLF20_04545"
     CDS             complement(7943..8731)
                     /locus_tag="PLF20_04545"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOX80311.1"
                     /translation="MQNGKKIFFNSDQHFGAPTAKESKVRERIFIEWLEHIAPEAEEI
                     YLMGDLFDFWFDYKAVVPKGYTLLLGALARITLSGIPVHYYRGNHDMWTFYYFEEELG
                     FIMHRQPEIVLLDGKKFYMAHGDGLGPGDRGYKFIKKVFENKINQWLFRQLHPDWGIP
                     LARFWSGRSRYANLERESREREDGKYDDQQWLLNEPLPQYALHILQQIPDIRYFIFGH
                     WHLPVALQLTDNCSYFNIGDWITHFSYLTFDGSVVEAKRWKPDI"
     gene            complement(8894..10624)
                     /locus_tag="PLF20_04550"
     CDS             complement(8894..10624)
                     /locus_tag="PLF20_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HOX80312.1"
                     /translation="MNDRRTNHFEENSPNEPDKTGLNFIEEIIEEDLRKGKNRGRVHT
                     RFPPEPNGYLHIGHAKSICLNFGLAQKYGGFTNLRFDDTNPTKEGEEYVDAIKEDIRW
                     LGFSWDDRDYYASDYFDQLYEWAEKLILEGKAYVDDQSAEEISRQRGTITTPGIESPY
                     RNRSVEENLNLFRRMRAGEFPDGHCVLRAKIDMSHPNMLMRDPIMYRIIHTSHHRTGD
                     KWCIYPMYDYAHGQSDYIEGITHSICTLEFEIHRPLYDWFLDQIVTTEYRPRQIEFAR
                     LNLTYTIMSKRKLLELVKEGYVKGWDDPRMPTICGLRRRGYTPASIRNFAEKIGVARR
                     DNIIDVSLLEFSLREDLNKTAQRRLAVLNPLHLIIENFPQDETELLPAANNPEDTGAG
                     IRKIPFSRELWIERDDFMIDPPKKYYRLYPGSEVRLKYGYIIKCQRYETDPESGEVKT
                     VYCTYDPESKTGGATANRKIKGTIHWVSKKHALKAEIRLYDRLFIKPDPDDVAEGESF
                     INYLNPDSLKIMNAWCEPALMDATTDKHYQFERIGYFYLDPDSCPGYLVFNRTVSLKD
                     SWSKMSQNEE"
     gene            10840..10910
                     /locus_tag="PLF20_04555"
     tRNA            10840..10910
                     /locus_tag="PLF20_04555"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10872..10874,aa:Cys,seq:gca)
     gene            11279..13036
                     /gene="recJ"
                     /locus_tag="PLF20_04560"
     CDS             11279..13036
                     /gene="recJ"
                     /locus_tag="PLF20_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOX80313.1"
                     /translation="MEKVWVHKPAGDAEIIRNLQKALNINGALASLLVQRGVRTFDEA
                     RSFFRPDLDDLHDPFLMKDMDRAIERIDKAIRNQEKILVYGDYDVDGTSAVALVYTFF
                     HEFYDNMGFYIPDRYAEGYGISYKAIDYASENDYRLVIALDCGIKAVEKIKCACTKKI
                     DFIICDHHRPGDELPPASAVLDPKRSDCTYPYNELSGCGVGFKLIQAYSRFKGWSSQD
                     LVKYLDLVAVSIAADIVQITGENRILAYYGLKLINSNPRPGIEALLGISGIKRTNEKP
                     DRIFTRELNINDLVFIVGPRINAAGRIESGNNSVELLISQSLEEARTLAEQINNCNTE
                     RRNLDSLITQQALDMIDEQETLSKAFTTVLYHPNWHKGVIGIVASRLTEIYYRPTVIL
                     CKSNGLITGSARSIKDFDIYDAIDACSDLLEHFGGHTYAAGLSLKPENLPRFTSKFEE
                     YAREKFNGNHPAPEIEIDQVINLSDITPSFFNILKQFAPFGPGNMSPIFATHKLMDTG
                     YSRIVGKNHLKLSVVHPHISGYPVSGIAFGLSKYFEDIQKGQPFSICYHIEENEWNGK
                     TNLQLNVKDIKFEPLDHEF"
     gene            complement(13330..13403)
                     /locus_tag="PLF20_04565"
     tRNA            complement(13330..13403)
                     /locus_tag="PLF20_04565"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13367..13369),aa:Pro,seq:cgg)
     gene            complement(13450..13523)
                     /locus_tag="PLF20_04570"
     tRNA            complement(13450..13523)
                     /locus_tag="PLF20_04570"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13487..13489),aa:Ile,seq:cat)
     gene            13622..>13885
                     /locus_tag="PLF20_04575"
     CDS             13622..>13885
                     /locus_tag="PLF20_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80314.1"
                     /translation="MNPVNNIIFDFGGVILNIDYKRSLNAFLKLGIQWEDSALEGFNR
                     SDAYIGLELGNICPENFFDELRKYFPESVSNKQLKDAWNAILLD"
BASE COUNT         4061 a         2682 c         2809 g         4333 t
ORIGIN      
        1 tatctggttc ttccatgcct accagattta ccgaaatctt cagctccgac gaccaaggcc
       61 ctataccaca aagataagtg ccccttatct tcaaacgtgc agttccccag aaatttggat
      121 tccatactac ttctatcgta gtatctgctg gcgtcatcaa ccagccagct tctggtggat
      181 ttaattccca ggtgtacgac aatgcaattc catcgggtgt gagcgaataa aaggaagtgg
      241 tagtagtata ggtattgatt tgctgtggcc cttttggctc tgcaggcgct ggtggtatct
      301 gttcttcatt acaatccttg atacggtaaa accaaacgtc aacccaatta taaagtgttg
      361 gaacattaat tagtaatata taatccccat attcaatttg agcagccgct ccccaataag
      421 tacctactac attaatacat ttttccttga tgatactccc atcctgacca ttgagcaata
      481 ccagccacat ataataataa tcactaaaat ttgaatttac gtctccatca tgagaatcag
      541 tcatagagaa aatcatcaga ttgccatctt tttctttgaa cattttataa actatttctg
      601 tgcctgatcc tccaagacat ttcgtccaga tgatgtttcc ctcgaaatcc gtctttataa
      661 cccaggcatc attataaccc atagcgttat tgtgaaaacc agtaaccaat ccaccacctg
      721 cgcttgcata acctcccaat acatatccat ctgacacttc gagtaaacta gaaaatccat
      781 catacccagg cccaccgtaa catttcgtcc agcatacgtt cccaaccgaa tccagcttga
      841 taagaaatgc ttcggcattc ccataacagg tatcacagaa cagattgccc agaccaggct
      901 tataataatc gaaagaaccc cctatcaata gacctttatc agaggtctcg attatggtag
      961 aaacgatatc gataaaatga ttgccaaaga cctttgacca taatatttgg ccctcatcgg
     1021 taagttttac aacccacaca tcgacggcac caaaattctg acccacatct ccatcattag
     1081 aagttgacca taccacaaag acaaagcctc catcataagt cattactgca tcctttaatt
     1141 catcagttct attccctcca taaatatgat tccatacaat attattgctg ctgtcgagtt
     1201 ttaatgcaaa tgcagaaatg ccacctaaat atggattgaa actaaatata tcatcactgg
     1261 aaatggtact gcccagtaaa tataaaccag aatcgggagc tgggaaaaca ttgttgaaat
     1321 attcaaatcc actacctcca aacccctggg tcgataatat attccccaaa tagtcggtct
     1381 taagtaacca agcatctctc cctccattcg gtgtagtacc taaattctta gaagaagaag
     1441 atcccacaat ataaggagag ttatctatta tttttatatt ataagcataa tcattgcccc
     1501 atcctttata aggatgccct acaactattt tccagttttg tgcttgtaat ggaagatcgg
     1561 aggttattat aataaaaaga cttgcaatca aagtaaaaaa tatttttttc ataatagttc
     1621 gttttttagt gtgagatata gggtagcagc gttttatgct gctcctcttt aaaaatatca
     1681 gtttttaatt ataaaaattg atttggccga tacaagcaat cgtcaaattg cttttcattt
     1741 tgttgatact attaaggaag agaatctaat cactgctgtt gagttagatt cctcccggct
     1801 ggtaataccc attcccgaag acttgcccta aaggagagtc aagtttagaa tcagagatga
     1861 atatgcagcg ggggagtgat aaaatattgg tattcattat ataatatatt caggtcgttt
     1921 ttcgttcatt aaatttgaat catcttatat atgtaagaat catttttgag ggtagaagta
     1981 atccttatag ataaaaattt tccaacaaat acaaaatttt ttcagaatct tgctcttcat
     2041 ggtttggtaa aatttagacc ctcaaagata aaactaaaat tttaaaaaac aaagagagct
     2101 gaattccgaa taatggtgga ggtgaaaaat taagtcatga gatttgatag aagggaatcc
     2161 tcctgtcgta cacaaagagt ttaaagctcc ggaattgtca aagatcaaaa tatttccgat
     2221 ggatagagaa aattattaaa atttgcaaaa gaattgttgt tgactcatga aaaagatttt
     2281 tttatttatt gctttgattt tgagtcttga acaggccgta gcccaaaagc acttttattt
     2341 tgaattaagg gctttcgata aaattcagat caatggtaat attgacgcac agattagtcg
     2401 tgcgggcgaa gaagatgcca ccctcagtgt taatggaatc gagcccgata gagtttccat
     2461 agaggttgaa ggccgtactt tgaagattaa gataggattt ttcgaaggaa gggatgctct
     2521 tgtgaaggtt tggatcaatt acaaagaaat cagggaggta aatgcttcca atggtgcgag
     2581 tttgcatttt actgagccca taaaatcaaa ttatatgaag atatcggctg gcacgggaag
     2641 ttcaatcgat gcacaaggcg agtttagaat tctcgaaatc aaagcttctg ccggaagcaa
     2701 gataaatata agaggcaagg cagatattgc agacatcagt gcaaatacca ctgccgaggt
     2761 tcaggctgaa ggtctggttt gtcaggaggg cattttaaag gcctataccg gagcaaagat
     2821 taccattcaa atagagcaga gcctcgaagc aacctcagcc actggagccg aaatcaccgt
     2881 aaaagggaat cctgtcattg agaaagtctc tacaagcaca ggtggaattt taactcgcat
     2941 aaaactctga aatgaccgaa tggtgacatt gaattttttc gataaaaaat atttcgattt
     3001 tctaccgaaa actatagtcg gtttcccggt tttttgaaag agaaagttta ccactataag
     3061 cgtccttagt taatcttaaa acaagcatga tttgggtttc aaatttttcc gatcgattga
     3121 atgctcattt tcgaactttt tccggtttat agtaatttcc tttaaaattc tttttggcca
     3181 tttttcgatt ctgtatattt atcgataaga taggtctgca aggctttatg gagtgccata
     3241 actacaaaat attcatcttc aatggtttgc gattctatga aaccctctca ttggaagacc
     3301 atcttccaat aatttatgct ccagttttcc ggaatacact tttaattgag ccatgaaatc
     3361 aacgaaatga tttgcttacc acattgttag atcgccttaa tcgaacagac tacttttttg
     3421 ttgaggattt gcttgtcaaa actgacgaaa gcaaaaccca ctggtggttg cttgtgtttt
     3481 ctatttattt tcagtgacgg aggtagaaca ttcagctata ttaccatctg cctgtcaaaa
     3541 atcatcggga gtagtttctt cgagcacctt tggattttac tttcacctaa tgacgattct
     3601 gattttcgag cattggtttc gactccttcc ttggccagaa acttacgagc agcttcttca
     3661 tgattattga taatcggaat aatagtatac aaaattctgg accccgattt ttctataatc
     3721 gaaaatcatc gtgcagctaa aagtttttag aaactccgaa ctaaattcaa accatccgct
     3781 gccgggtttt atccaaaggc agtgcaaagg cttataagct caggaaagaa tccatacccg
     3841 gcgatttgtc ctttttattc gtattgtgcg aaaacaccaa gagtgcgcca ccagcaagca
     3901 tcacaaggcc aacgataagc gacagattat tcgttttcga taaaaataca aaaggatgga
     3961 acaattgatt ggggttaagt ttaaatttca aggtcgaaaa tatctggtaa atgtaatcca
     4021 tgaaactaaa atcgctcgta aagatgagga ttccgaaaaa tccaatggtt aaagccaaag
     4081 caagcaaggt gcgaaatccc aaattgaaat ttgcattgat agatagtttt tcgacattct
     4141 ctctggcaat ctgatccatt accttctgtg taaaatcttc ggaaggagac tcggaaggga
     4201 ttttactgaa cacctccctg agcaggtcgt cggacttatt ggtttgtata ttcatgattt
     4261 tgagttttta tattcatagg tagaggtgga ctgattgaga agaagctgaa cacccgcaaa
     4321 aatacgtttt cgtattcggt gaagttttac tttaacgtta gagacggaga gcgaggtgat
     4381 atggctaatt tcttcgatat tcaattgttg attataaaac aaatttatca aagcagcgtc
     4441 gtcgcctggt aatgatttca gaacgtgctg gaccaattta agctgttcat cggtatcgag
     4501 catttgcaaa ccggctacaa aattgtcttc agaataattt tctatcagat gatggtcgat
     4561 ggctacaaat tccatttttt tctttctttt tttcgagata gccgtattgt aaacaattcg
     4621 ataaagccaa gtactgaatt tcgattcgaa tttgaatccc ttgagcgcct gataggcctt
     4681 aacgaaggca tccatagcta tttcttcggc atcttcgcgg ttacccgtaa tctgatacgc
     4741 aatgctaaaa gccgtttgtt tgtatttttc gactatttgg gcaaatgcaa attgatcacc
     4801 tcgaagagta cgttgaatta atatttcgtc gttgatttct gccattggga gtttgacgcc
     4861 ttcatttcgg gttggttaca aacttagcag taaatattta gggattcaac attttcgtct
     4921 caggggtgta accggtttgg atttgttgcg tcaaaaacac gtggacaaca aatgctgaca
     4981 ttgttaattt taatattatc gtgatatgaa tggcgagatt ttaattccct taggtttttt
     5041 tgcaatggta gtagccattg tttatctggc attaaggcaa agagagcgta tgaagatgat
     5101 caatcagggt atggatccag cggcaagcaa tacgggcttg gagagcacgc ttcaacttcg
     5161 tctggggctc attttcattg gaattgctgt tggttttttg ctgggagata tcttcagttc
     5221 gcttcactgg cttaaggaat gggtagcgta catatcaatg gttactattt tcacgggcat
     5281 tgccctcatc gtgagttatt acctgattaa caaaattcct ccacgcgata ggaaagaata
     5341 atgatccatt tgaggattat tcgagtgtta acggctgtaa agggaaactt ttcagggttt
     5401 tatactgagg tcctaaaggc gtgagtgtgc tccaaataag caatacttca cgaatggcaa
     5461 aaggattgaa aacatgttga gatgatgatt ccattaggtt tttcagcaaa tcgggctggc
     5521 gaatgaattt aggacgtccc agggtgaggt gtggtgtaaa tgaattattc ttttttgatg
     5581 ggtcagtgag gaaggcatgc tcgatctctt gagctaaact ttcgagagtt ccagtggatt
     5641 cattgatgct taaccataat gctcgagctt gttttaggct gccaaaaatt cccaatttat
     5701 tgggctgaat ctgaaacgat tcatattttt tagtgattcc atctaactga tggataatgg
     5761 aagatatgtg gtcttcagga gtatctccaa aaaatttcag tgtaatgtga aaattttgag
     5821 tggccgacca tgtgatgtct tcatttctta gctgacggcg caagaatgaa aaataataga
     5881 ggaaatccga atcgggttgg taatcgacgg ctataaaaat acgttttagt gtcattttag
     5941 tgccaaatgt aaatattgca tttattcatc ttctggattc agttttttca tgatcttgat
     6001 tctttcgtcc agtagctgat cttcttcttc ctgatttaga ttaccttcgg ccaatagttt
     6061 atcgatttgt accaggcgcc gtgtaagcgt gccaatagaa ggtttttctt cttcgggcag
     6121 gggtcgaagt aaggtttccc tttctgattt tttgggtata ttaacaggtt cgccaagttg
     6181 ttcggaaaga tttttaataa aaagattcaa catattttca aaaggtttac ccatttgttg
     6241 aaatatacta cgcgcctggg gatcgtattt cattctctca aaattgctga taaggaaatc
     6301 gagctggctc atcatgaagg gatcgaagcc cgcaatcgtt tccggctttg cttgttcctt
     6361 cagcttcttg aacaagcgga tgagtttttc gattttatct tcgaaattat cgtctgattc
     6421 catgtattaa tctttcgtga tgtgagcaaa ttgaatatag aactcagaga tggtttctat
     6481 tcccttgaag aagttcttta cggagaagtt ttcgttgggt gaatgaatag cgtcagattc
     6541 gaggccgaag cctaaaagta gtgattttat tcccaatact ctatcgaagg tactgatgat
     6601 gggaatagag cctccactgc aggtaggaat gggatatttg ccaaaggttt tcaaataggc
     6661 tttttgagct gccttatacg ctggaatgtt ggtgggtgct aaatatggat agcctccgtg
     6721 caatgcggtt acttttagtt tcactgtatt gggaaccatt tcatgtaaat agttttcgaa
     6781 taaattggcg atttttttgg gatcctgatc aggcaccaga cgcatggaga ctttagccga
     6841 ggccttcgac ggtaatatag ttttcgatcc ttcgcctgta tatcctcccc atattccaca
     6901 aacatcaaga gagggcctaa ttcctatacg ctccagaggt gtaaatccac tttcacccca
     6961 gaggcagtta attcccaatg attttttgta agattcctca tcgaaaggag caagattgaa
     7021 ctctattcgt tcttcttcgc tcagttccct tacatcgtcg taaaatcccg gaatgattat
     7081 acggccatgg tcgtcgtgaa ggttgcctat cattctggat aaaacatgaa tgggattgtg
     7141 tacgattcct ccataaagtc cggaatgcaa atcacgattt ggccccgcaa cttcaacttc
     7201 tatgtaagca aggcctctta atccaactgt aatagaagga gtatccggag caatcatgct
     7261 ggtatcggaa agcaatatga tatctgcctt aagcatgtct ttatgttctt ggcaaaagtc
     7321 ttctaaatgc ggggaaccaa tttcttcttc accctctatc ataaatttta cattgcactg
     7381 caattgacgg gaatttacaa ggtattctat ggcttttacg tgcatgaatc cctgtccttt
     7441 gtcgtcatcg gcacctcgtg cataaatctt cccttccctg atttcaggct cgaaaggagg
     7501 tgattgccat aactcgatgg gatcaactgg catgacatcg gcatgggcat ataccagcac
     7561 agttggaaaa tccggtgata aaattttttc cgcataaact accggatggc caggtgtgtc
     7621 gaagatttcg actttatcca tgccggcttt caaaagagca tttttgtaat actcggctgc
     7681 gcgatacata tcatctttat gttcgctcag gctgctgatg gatggaatct tcagaagccc
     7741 gaataattct tccatgattc ggtttgaatt tttttcgagg tatgtggtaa tatcttccat
     7801 attataggtt ttcgctcatg ctaaagccat ctttgttcct gtcggtatcg tatgcaaaga
     7861 tagcaaagct catccatctg ttcaactttt tttgcaagcc ctctgaaggt tgagacgaaa
     7921 gaatataatt ggaaagattt tttcagatat ctggcttcca tctttttgct tccaccactg
     7981 agccatcgaa agtaagatag ctaaaatgcg taatccagtc gccaatattg aaataggaac
     8041 aattgtcagt aagttggaga gctacaggca aatgccaatg tccgaaaata aaataacgta
     8101 tatcgggtat ttgttgtaga atgtgtaaag catattgagg aagaggttca ttcaatagcc
     8161 attgctgatc atcgtatttt ccatcttcgc gttcgcgtga ttccctttcg agattggcat
     8221 accggcttct tcccgaccag aatcgagcca atggaattcc ccagtctgga tgcaactgcc
     8281 tgaataacca ttgatttatt ttattttcga atactttttt gatgaattta tatccacgat
     8341 ctccaggacc caggccatca ccatgtgcca tataaaactt cttcccatcc agtaaaacaa
     8401 tttcgggctg ccgatgcata ataaatccca attcttcttc gaagtaataa aatgtccaca
     8461 tgtcgtgatt gccacgataa taatggacag gaattccgga taaggtgata cgggcaagag
     8521 ctcctaataa aagtgtgtaa ccctttggaa cgactgcttt ataatcgaac cagaaatcga
     8581 acaaatcgcc catcaaataa atttcttcgg cttcgggtgc aatgtgctcg agccattcga
     8641 taaaaattct ttccctcact ttactttctt tggcagtggg tgcaccaaaa tgctggtcgg
     8701 agttgaaaaa tattttttta ccgttttgca tcttcatgca aatttatcaa gaaatgtttt
     8761 ggccattatt gattttggcc gagtcatctc gggctggctg aaaaataatc ggattattat
     8821 gtgaagatgt tagtgcaaaa aaaaataatg ataaggaaga tttgttaatc ttgttttgtt
     8881 ttatgcaatg tgattattct tcgttctgac tcattttgct ccatgaatct ttcaaagata
     8941 cggttctgtt aaacacgagg tatcctggac atgaatcagg atcgagatag aaatatccaa
     9001 ttctttcgaa ctgataatgt ttgtctgtgg tggcatccat taatgcgggt tcgcaccagg
     9061 cattcattat tttgagtgaa tcggggttca gataattgat aaaactttcg ccttcagcta
     9121 catcgtcggg atcgggtttt atgaaaagcc tgtcataaag tctgatttcg gcctttagcg
     9181 catgtttttt cgatacccaa tgaatagttc ccttgatttt tcggttggct gttgctccac
     9241 cggttttcga ttcagggtca taagtgcaat atacggtttt tacctcccct gattcaggat
     9301 cagtttcgta acgctgacat ttgataatat atccgtattt tagtctaact tccgaaccgg
     9361 gataaagacg gtaatatttt ttgggaggat cgatcatgaa gtcgtctctt tcgatccaca
     9421 actcgcgtga aaatgggatt ttgcgaatgc ctgctcctgt gtcttctgga ttattggcag
     9481 ccggaagaag ttcggtttca tcttggggaa aattttcaat gataagatgt agaggattca
     9541 atacagcgag tcttcgttga gcggttttgt tcaagtcttc acgcaagcta aattccaata
     9601 gcgaaacatc gatgatattg tctcggcggg ccacaccgat tttttcggca aaattgcgaa
     9661 tagatgctgg ggtataaccg cggcggcgta aaccacaaat ggtaggcatg cgtggatcat
     9721 cccatccttt tacataacct tcttttacaa gctccaaaag cttacgttta ctcatgatag
     9781 tataagtaag gttcaggcgg gcgaactcga tttgtcgagg ccgatattca gtagtgacta
     9841 tctgatctaa gaaccaatcg tataaagggc gatgaatctc gaattcgagt gtgcagatgg
     9901 aatgagttat gccttcgatg tagtcgcttt gaccatgtgc atagtcatac atgggatata
     9961 tgcaccattt gtcacctgtt cgatgatggg aagtatgaat gatccgatac atgataggat
    10021 cgcgcatgag catgttggga tgcgacatgt cgatttttgc cctcaaaaca caatgtccat
    10081 caggaaattc acctgccctc attcggcgaa acaggtttaa attctcttcc acactacggt
    10141 ttctgtaagg gctttctatt ccaggtgtcg tgatcgttcc tctctgacgg ctgatttctt
    10201 cggccgattg atcatccaca taagctttcc cttcaaggat gagtttttcg gcccattcat
    10261 atagctgatc gaaatagtca gaagcataat aatctctatc gtcccatgaa aatccaagcc
    10321 agcgaatgtc ttccttaatg gcatccacgt attcttcgcc ttctttggtg ggattggtat
    10381 cgtcgaaacg gagattggta aaccctccgt atttttgagc caacccaaag ttaagacaaa
    10441 tggatttagc atgcccaata tgaaggtacc cgttgggttc gggtggaaag cgcgtatgca
    10501 ctcggcctcg atttttccct ttgcgtaaat cttcttctat gatttcttct ataaagttca
    10561 atcctgtttt gtctggctcg ttaggagaat tctcctcgaa atgattcgtc ctgcggtcgt
    10621 tcatccaaaa attgttgttt tgatggctgt aaagctttgt acgagtggaa gtaaaactat
    10681 agatttcgct tgcaaaagta attatacttg aaggattgga caaggaaatt acaatggaat
    10741 aaaatatata ttatagatat tcaatagatt atgattataa cgcaaagtat gattttgtta
    10801 atcgagtggt ttgtcctaat tttgtccttc catatttaag gtctcgtggc cgagtggcta
    10861 ggcacaggtc tgcaaaacct cgtacagcgg ttcgaatccg ctcgagacct caacattgag
    10921 atagattgct gtcatattga agtagcaatt ttttaatcca cattgacttg ggaagagcta
    10981 tgactatatt gaaagcagcg aatgctcatt tcatagagat aatttgttgt gttaatttat
    11041 accttttcga tgaccctctg atataggaac ggaggattct ttacttaagg aaagtgaatc
    11101 tttcagtcag tgaatgaaaa ttttaaaaga agaaataact cgtgcgggaa gaaggacagc
    11161 aatttttaga attaagtttt ttagattaat ttattgcttt tcaatgcttt atatttaatt
    11221 ttctattcta tggataggat tcgtgaagaa ggtgtaattt tacaacctga aaacaatcat
    11281 ggaaaaagtc tgggttcata aacctgcggg agatgctgaa atcatccgta atttgcagaa
    11341 ggccttgaat ataaatgggg ctttagcatc tttattggtt cagcggggag ttcgtacttt
    11401 tgatgaagcc cggtcattct ttcggcctga tctcgacgat cttcacgatc cttttctgat
    11461 gaaagacatg gatcgagcca ttgaacggat cgataaagct atcaggaatc aagaaaagat
    11521 tctggtgtat ggtgattacg atgttgatgg cacttcggcc gtagccttgg tttatacttt
    11581 ttttcatgaa ttttacgaca atatgggatt ttatattcct gatagatatg ccgagggtta
    11641 tggaatttca tacaaagcca tagattatgc ttcggagaat gattataggt tggttattgc
    11701 actcgattgc gggattaagg ccgtagaaaa gataaaatgt gcctgcacaa aaaaaataga
    11761 ttttattatt tgcgaccatc accgccccgg agatgagctt cctccggcga gtgccgtgct
    11821 cgatcccaag cgctccgact gtacttatcc ctataatgaa ctctctggat gtggagtagg
    11881 ctttaaactt atacaagcat atagtcgttt taaaggatgg tcatcccaag atctggtaaa
    11941 gtacctcgat ttggttgccg tgagcattgc ggcagatatt gtgcaaatta ccggagaaaa
    12001 caggatacta gcctattatg gattgaaact aatcaattca aatcctcgtc ccggtatcga
    12061 agctttattg ggaataagtg gaattaaacg gaccaacgaa aaacccgaca ggatttttac
    12121 cagagaattg aatataaacg atctggtatt tattgtgggg ccacgcataa atgctgccgg
    12181 gcgaatcgaa agcggaaaca atagtgtaga acttcttatc tctcaatcat tagaagaagc
    12241 ccgaacactt gccgaacaaa tcaacaattg caatacagaa cggcgtaacc tcgacagcct
    12301 catcacacag caagctctgg atatgattga cgaacaggag actctgtcaa aagcatttac
    12361 gaccgtgttg tatcatccca actggcataa gggggtgatt ggaattgtgg cttcgcgtct
    12421 taccgaaatc tattatcgac ctacggttat tctttgcaaa tcgaatggat taattaccgg
    12481 ttcggctcgg tctattaaag attttgatat ttatgatgcc attgatgctt gcagcgattt
    12541 gctcgaacat tttggaggtc atacgtatgc tgctggactt agcttaaaac ccgaaaatct
    12601 tcctcgcttt acttcaaaat tcgaagaata tgcgagagaa aaattcaatg gcaaccatcc
    12661 agcacccgaa atcgaaatcg accaggttat caacttaagc gatattacgc cttctttttt
    12721 taatattctg aaacagtttg ctccttttgg ccctggaaac atgtcaccca tttttgccac
    12781 gcataaacta atggatacgg gctattcgcg tattgttggg aaaaatcatc tcaaattatc
    12841 tgtggttcat cctcatatca gcggataccc tgtttcggga attgctttcg gtttgtcgaa
    12901 atatttcgaa gatatacaaa aaggacaacc ttttagtata tgctatcata tcgaagaaaa
    12961 tgaatggaac ggaaaaacaa acttgcagct taatgtaaaa gacattaaat tcgagcctct
    13021 ggatcatgag ttttaatatt ggccagaact caaccctata ccaattcttc tttcatcctt
    13081 atacattttt ggttctttta agttttgatt tcctttgcag ttgctcatag catcttcgac
    13141 tgggaaacct aaacagaata ttttattggt tttgaacaca tttgacgata ttgctcattt
    13201 catttatgaa acaaatctta ccatctatca ttatcctgga aaatagaaaa gcatgcaggc
    13261 aaattcctgc atgctttaat agataggcag aagaaataat gaaaaagacc tcagcgatga
    13321 gaggccttgt cgggatgact ggactcgaac cagcggcctc cacgtcccga acgtggcgcg
    13381 ctaaccaact gcgctacatc ccgggataaa gaatgtattc gtacaaaaca agcttaaatc
    13441 gctgaattgt gcgcccacct ggacttgaac caggggccta ctgattatga gtcagttgct
    13501 ctaaccaact gagctatggg cgcaattttt tttaataaat tttgaaaacc cgagtgcaaa
    13561 tttacatctt tgctcaaaaa tatcaacatg aactttgata aatatttaaa atgtattgaa
    13621 aatgaaccct gttaacaaca ttatcttcga tttcggaggt gtaatcttaa acattgatta
    13681 taagcgttct ttgaatgcat ttctgaagct aggaattcaa tgggaagact ctgctctgga
    13741 aggatttaat cgtagcgacg cttatattgg gctcgaactt gggaatatat gccctgaaaa
    13801 ttttttcgat gaactgagaa aatactttcc tgaatctgtt agcaacaaac aactcaaaga
    13861 cgcatggaat gccatcttgc tggat
//
LOCUS       DAOORU010000048        13732 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_5478_length_13732_cov_5.551583, whole genome shotgun sequence.
ACCESSION   DAOORU010000048 DAOORU010000000
VERSION     DAOORU010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13732
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5478_length_13732_cov_5.551583"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="PLF20_04580"
     CDS             complement(<1..458)
                     /locus_tag="PLF20_04580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX80315.1"
                     /translation="MEISTEFTEKANRDYQWIQQALHGGDQRAYAKLMDNYRESLYYL
                     MLKMTNDVDDAEDMMMEAFSKAFKNLHQYTPIYAFSTWLFRIAANNCIDFMRKKKNTL
                     SLDASNYTYNCDDTGFDQLSWGLPDDCLDPEQALMKKQSNQFLREIVDQL"
     gene            complement(449..1651)
                     /locus_tag="PLF20_04585"
     CDS             complement(449..1651)
                     /locus_tag="PLF20_04585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX80316.1"
                     /translation="MEGVFIFQQRLLNNFAAIIILRLMDFNNLPQLPYFWLFIIYSSV
                     IIIQLFYYWGIFIRLAFHNYRPAIIEQWPEVSVVICARNEYENLKKNLPYILNQDYPV
                     FEVVVVNHTSDDDSLYVLRNFQKEYPNLSIVNIEKDLNFFSGKKFPLSIGIKSAKHEI
                     ILLTDADCRPASAQWIKTMVSAYTHETSVVLGYSGFYETKGLGNIIQRFNAFHTALQY
                     LSLAMAGMPYMGVGRNLSYKKSLFYQVNGFISHYSLESGDDDLFINQVATKSNTRCSL
                     DSASFTFSSAKKNFREWIRQKRRHLTTGLHYKPWHKFVLGLYSLSQFLVFFFFIILLL
                     LGFPWPLVVSMFILRLISQLVIYGNAMKKLQTGKLLLISPILEFIEMIVLHYVVLTNR
                     FVKRKQWK"
     gene            1756..2886
                     /gene="tgt"
                     /locus_tag="PLF20_04590"
     CDS             1756..2886
                     /gene="tgt"
                     /locus_tag="PLF20_04590"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOX80317.1"
                     /translation="MKFNIKFTDRQTQARTGCLDTDNGLVLTPAFMPVGTAGSVKAVH
                     AKDLFEDTSALIILGNTYHLYLRPGLEVLKKAGGLHRFSSWNGAILTDSGGYQVYSLA
                     LRRKLTPEGVVFQSHIDGSRHLFTPERVIDIQRIIGADILMAFDECTPYPCSYDYASK
                     SLQLTHSWLDRCLKRFNETQPEYHYDQSLFPIVQGSVYEDLRLQSAEYIASKNAPGNA
                     IGGLSVGEPAEIMYQLTEKVCQILPSDKPRYLMGVGTPANILESIALGIDLFDCVLPT
                     RNGRNGQLFTLEGIINIKNEKWKNDFSQIDQEGTSFVDHLYSRAYLRHLFVTGEILGP
                     MVASLHNLRFYHWLMEESRKHIMKGDFGEWKNLMIPRLSQRL"
     gene            2911..4020
                     /locus_tag="PLF20_04595"
     CDS             2911..4020
                     /locus_tag="PLF20_04595"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOX80318.1"
                     /translation="MRKNFISFRLTVIDRYIIRKFLGTFFYSIGLLIIIVVVFDVSEK
                     IDDFLTRRAPLRAIIFDYYLNFIPYFINLFSSLFTFIAVIFFTSRLAERSEIIAMLSS
                     GINFFRLLKPYLISATFLVILTLYFSNFLIPHTNIKLRDFEKQYIKNPLRSKSLNLHL
                     QIEPGVFVYFESWDNARKNGYRFTIERIENSKLSYKLMSDQIKWDSIKSKWHIINYTE
                     RFIDTATFEQSLVFGSQKDTTLNLRPTEIIVDKEDMKIMNFNELKAFIEREKLKGSST
                     IKYYETEMHKRIAEPISAIILTLIGMALSSRKVRGGTGMHLGIGIGITFTYILFMQIT
                     TQFSVYGNMAPMLATWLPNIFFGIIAIWLLLKAPK"
     gene            complement(4093..4716)
                     /locus_tag="PLF20_04600"
     CDS             complement(4093..4716)
                     /locus_tag="PLF20_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX80319.1"
                     /translation="MKPQGKASREEILEAARKVFIENGYDGARMQEIADEAGVNKALI
                     HYYFRSKEELFQEVFLEAFIKVVPSVMVILNSDEPLFKKIEQFVSKYLDTLNENPLIP
                     GFILHELAHGHQHIDALIRGTGLNPQFFINQIYREIDAGKIKNCDPTQLIINILSLCI
                     FPFVAQPILKGVFFRNREDDYARFMLERKSDVVRFIIASIRKQQEES"
     gene            complement(4856..5953)
                     /locus_tag="PLF20_04605"
     CDS             complement(4856..5953)
                     /locus_tag="PLF20_04605"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80320.1"
                     /translation="MRKILLSVLLMSLGGWLSAQSLQLKTLDGKLIANDATLYWGGLP
                     TEYMVARILVSNTSTEAKNVFVKKYEEFAAKDSTATFCWGACFPWFVNVSPEGIVIDA
                     GGTSTDFTADYDAGGVLGHTRLKFTFFVENNPNDSVSFRVEFSPSYLSLRNTEDSIPT
                     ASVLNVVGSYTEVIQYDLILTNHSKETKNVSMHKIHLSVIEGTEMYFCWGDCYPPDVF
                     ELPEPMVMAAGESSQGLSVDYDPKSHSGTSTITYVFYDFDHPADSVWVDFAFDGQAAG
                     IDYLASDILKISYRAFSHELFIEWENEISYGSKNLQVIDMSGKVVFTKTLSSNSRQQT
                     ITLPYLSQGIYMIVINLPDGMVYRNKFLISR"
     gene            complement(6056..6850)
                     /gene="pgeF"
                     /locus_tag="PLF20_04610"
     CDS             complement(6056..6850)
                     /gene="pgeF"
                     /locus_tag="PLF20_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOX80321.1"
                     /translation="MLIDKDEPICCYFENLSRFDGEILHFVSTRRFLKDQQFSISFNG
                     SLSKDRVRVNRKVLFSKFGLDVSSSVFTTQVHGNKVERISIKEKGRGVNEVLKTCLGS
                     DGMITQHQEICLVIQMADCVPLLFFDPIKKVIATAHAGWRGTVMKIGAKVIEEFRNVY
                     DSDPADILAGIGPSIGPCCYEVGNEVISKVEEAFRDTKGILLNHPKFSRPVFDLWEAN
                     RRILIEKGINPRNIEMANLCTRCHSDMFFSARAGDEGRFGSFIMLK"
     gene            7280..11911
                     /locus_tag="PLF20_04615"
     CDS             7280..11911
                     /locus_tag="PLF20_04615"
                     /inference="COORDINATES: protein motif:HMM:NF037268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease domain-containing protein"
                     /protein_id="HOX80322.1"
                     /translation="MSKILGLDLGVNSIGWAVVDDEKASIIDCGVRIFPEGVENLGQG
                     EKETSKNAQRREKRQLRRHYYRKRLSKIKLLETLIPLKMCPLSLNSLKKWKNWNNELK
                     SDGRKFPNDPEFIEWLKMNPYALRKKALEEDLSLEQLGRIFYHFIQRRGFYSSRKGKD
                     SGKIYQGENNTIGILETEKEIGYNKTLGTTLYEMILPEHQTYIYRTDENRRPLRARNR
                     YTLREMYVKEFHAIWEKQAPHLGLNDKTVTIFKSFHLSGNNDSERNKQRINKIYLQYG
                     IENVTITGKTIKVAQQISLKTLFGGRIEINDETHEILYRSQDSLLFWQRPLRSQKHLL
                     SKCTFEKRIMFDKTTNKWIETGPTPCPISHPDFELYRAWQLINNIRYGRKEQPLDASQ
                     RQTVLNVISYQDKVFDFKVIIDALKLQNEYFNYDADQKVSGCPTIKKLKSLFPTEIWV
                     QNKHEIWHDFMFFEDEELLTQRLIKKYHFTEEKAQKSAKITLEDGYASISLKAIHNIL
                     PFLEKGFIFQQAVLLGGIKNTFGSRWEYFKDYHSDIEQFIKDLCNGKKYHEGEIIQNI
                     KEYLSSTVNNFGFNPNDKAFLKLYHPSREVGPEKKGDYISPVENLRNPLVQIGLNEMR
                     RLVNYLLKKYRSMPEFGPDFCFDRIHVEVGRDLKNNKKRRQEIRKENAINEQKNKEAR
                     ERIIEAGLKPSRDNIIKYLLYSEIEQKNGIVICPYTGKTIRLSDLLGPHNFFQIEHII
                     PYSVSLDDSFGNKTLCEANFNRSKGELTPYQFYLKNSSKSLWNADSWEEIEQRVFQIL
                     PYKKARRFTSKKPAMLDDFIQRQLNDTRYISKKSVQILREICDDVRSFPGQLIADLRR
                     LWGLNDILSEPITDIPLNDLNINESEPTIHYLIVDENNHVKKAIPKINFKPIPMDNEL
                     AIPGEIKKETGNKFYFESDSKYYASLKIPANGFETGKYWAFIKAQAAQEFIPRFTPQP
                     QISENVIIIKGNLKKKRFINESHGINFNTNKIIEDGYYWAIIPVIKAEIKQNDINDNR
                     KNENKISIQGRVSNQKFISSIYSCEAQLINGFYTALLDFDVNNADFLPALNLPRPPEN
                     YYTLNGTVDSQGIFCPDMDYNWKLKADVAPGRYFIYFKIQEIINFQPIENPKPIIDKN
                     NNEKIIESLIRVDTETGEIIIDPKKNREDQRHHAIDAIVIASATTSYYQKLSTYFGNL
                     KNKQRNLAPKPKFEQPNPHFQNAVKEAISKILVSYRTNYRVLVPVYKRIRKNHDTYVS
                     KGYSVKGQLHKDTFYGGYILDQGEGKIVSAAKYLPGIKPQQYHVRCNITEIKDHKHVA
                     KIVDTTIKKLIENRLQNVFNIDISKPYNIPNGFFFDKDKNPAIRLPNRKGGDPVPVYT
                     VRIFEYKGNAVKLKPNINCYVDPRNNHHCIIYKDVEGNLKFEMVKFWDAVNRLRHKQP
                     LYQLPPDGSQIIATVQENDMFIFNYLYASDISEDMKIPYEELSKYLYRVQKISSTEGG
                     IFLTFRHHLASTIKNPEEELRIQSLRSWERHQPIKVKINMDGTVNKIIK"
     gene            11908..12792
                     /gene="cas1"
                     /locus_tag="PLF20_04620"
     CDS             11908..12792
                     /gene="cas1"
                     /locus_tag="PLF20_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HOX80323.1"
                     /translation="MIKRTLCIQNAGHLNLNNNQLVFTDKEGIKKTAPIEDIGYLIIE
                     NRYVTISVQLITALNSNNAAIIFCDEKYLPTGMLLNLDSNQVQTEIFHTQIEASEPLK
                     KNLWKNVITCKIRNQAALLHKLNKNDQPLKIFANEVMSGDTSNREALAAKYYWHELWG
                     SGFIRDRFGPPPNSLLNYGYIIIRSAMARALAGSGLLSTLGIFHSNRYNAFCLADDMM
                     EPYRVYVDELVYEVWYKTDKPEPDKEFKQMAIQLLARDVNLEKTTRPLMLAMQQSSAS
                     LARCYGGKQRKLSFPDFK"
     gene            12840..13145
                     /gene="cas2"
                     /locus_tag="PLF20_04625"
     CDS             12840..13145
                     /gene="cas2"
                     /locus_tag="PLF20_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOX80324.1"
                     /translation="MWLFVFFDLPVGTKKEIKEATRFRKNLEKDGFNMMQYSVYTRHC
                     PSRENAEVHVKRVKSIIPVKGQVSILMVTDKQYGEIYNFFGKTEKFLPEGPRQLEFF"
     assembly_gap    13491..13500
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         4674 a         2602 c         2501 g         3945 t
ORIGIN      
        1 ttaagttgat cgactatctc acggagaaat tgattggatt gttttttcat gagagcttgt
       61 tcggggtcaa ggcagtcatc gggtaaaccc cacgaaagtt gatcaaaacc cgtatcatca
      121 caattataag tataattaga agcatccagc gacaaggtat tcttcttctt cctcatgaaa
      181 tcgatgcaat tgttggcagc aatcctaaaa agccaggtac taaatgcata gataggagta
      241 tactggtgga gatttttaaa tgccttgcta aaagcttcca tcatcatatc ttcggcatcg
      301 tccacatcgt tggtcatttt cagcataaga taatacaacg attcgcggta attgtccatt
      361 aattttgcat aagcacgctg gtcgccccca tgcaaggctt gctgtatcca ttgataatct
      421 cgattggcct tttcggtgaa ttctgtactt atttccattg ctttctttta acaaaacgat
      481 tggtcaatac cacgtaatgg agtacgatca tttcgatgaa ttccagaatt ggcgaaatta
      541 acaataattt acctgtttga agttttttca tggcattgcc atagataact aactggctta
      601 ttaggcgtaa tatgaacatt gaaactacta atggccatgg aaatccgagc aataacaaaa
      661 taatgaaaaa gaaaaatacg aggaattgac tcagggaata taagcccagc acgaatttat
      721 gccaaggttt ataatgcagg cctgtagtca ggtgcctgcg tttttgtcga atccattccc
      781 tgaagttttt cttggctgac gaaaaggtaa acgaagcaga gtcgagactg caacgagtat
      841 tgctcttcgt agctacctga ttgatgaata aatcatcgtc gccagactcg agagaataat
      901 gcgaaataaa accgtttacc tgataaaaca gcgatttctt atagctcaaa ttgcgaccca
      961 ctcccatata gggcatccct gccatggcca gcgaaagata ctgcaaagcc gtatggaagg
     1021 cattgaaacg ctgaatgata ttcccgagtc ccttggtttc gtaaaagcca ctatatccca
     1081 aaacgaccga ggtttcatgg gtataagccg ataccatggt cttgatccac tgggcggaag
     1141 cgggacgaca atcggcatcc gtaagcagta taatttcatg ttttgccgat tttataccaa
     1201 tgcttaatgg aaatttcttg cccgagaaaa aattcaggtc tttttcgata ttcacaatcg
     1261 aaagattagg gtattctttc tgaaaattcc tcaacacata aaggctatcg tcatcactgg
     1321 tatgatttac cacaaccacc tcgaaaacag gataatcctg atttaaaata taaggcagat
     1381 tctttttcag attttcgtat tcgtttcggg cgcaaattac tacagacacc tccggccatt
     1441 gctcaataat agcaggtcta taattgtgga aggccaaacg aatgaatata ccccaataat
     1501 agaaaagctg gataataatt acacttgaat aaataataaa gagccagaaa taaggaagtt
     1561 ggggaaggtt attaaaatcc ataagacgga gtatgatgat tgctgcaaaa ttattcaaaa
     1621 gccgttgctg gaaaatgaaa actccctcca aaattatgaa caatttgttg ataagttaca
     1681 ccgcttggga aaccgctttc gataaaaaga cacgtaaaca ttaattttgc agccttaaaa
     1741 taaatgtctg ttttgataaa atttaacata aaatttactg atcgtcaaac ccaagcacga
     1801 actggatgtt tggatacaga taacggactc gtgttaactc cggcgtttat gcctgtagga
     1861 acagcgggca gcgtaaaagc cgttcatgca aaagatcttt tcgaagatac ctctgccctt
     1921 attattttag gcaacaccta tcatttatat ttaaggccag ggctcgaggt tttgaagaaa
     1981 gcaggaggtc tgcatagatt cagcagctgg aatggtgcta tactgaccga tagcggagga
     2041 tatcaagttt atagcctggc tttgcgccgt aagctcactc cagaaggtgt agtttttcaa
     2101 tcgcatatcg acggttctcg ccatctattt actcccgaaa gggttattga tattcagaga
     2161 attattggtg ccgatattct catggctttt gacgaatgca ctccttatcc atgctcctac
     2221 gattatgcct cgaaaagctt gcaactaaca cattcatggc tcgaccgctg cttgaaaaga
     2281 tttaacgaaa cccagcccga ataccattac gaccaaagtt tatttcccat tgttcaagga
     2341 agtgtttacg aagaccttcg cctgcaatcg gccgaataca ttgcttcgaa aaatgctccg
     2401 gggaatgcca taggaggctt gtcggtaggc gaacctgccg aaataatgta tcaacttacc
     2461 gaaaaagtct gccaaattct accatccgat aagccacgat atttgatggg agttggcact
     2521 ccggccaata ttctcgaaag catagcactg ggcatcgacc tgttcgattg tgtacttcct
     2581 acccgcaacg gccgcaatgg acagttattt actttggagg gcatcatcaa cattaaaaac
     2641 gaaaaatgga aaaacgattt tagccaaata gaccaagaag gcacttcctt tgtagatcac
     2701 ttgtattctc gcgcttatct gcgtcatctt ttcgtaactg gcgaaattct cggccccatg
     2761 gtcgcaagcc ttcataacct tcgcttttac cactggctga tggaagaatc gagaaaacat
     2821 atcatgaaag gcgatttcgg agaatggaaa aacctgatga tcccccgatt gagtcaaaga
     2881 ttgtaaattc gcactttgta tcccatattc atgaggaaaa attttatatc attccgactc
     2941 accgtaatcg atcgttatat tatccgaaaa ttcctcggaa catttttcta ttctattggc
     3001 ctgctcatca ttatcgtcgt cgttttcgat gtttccgaaa aaatcgatga tttcttaacc
     3061 cgacgagcac ctctgcgagc tatcatcttc gattattacc tgaatttcat tccctatttt
     3121 atcaatcttt tcagttcgct cttcaccttt attgccgtca ttttttttac atcacgactt
     3181 gccgaacgct ccgaaatcat tgcaatgctc agtagcggaa tcaatttctt tcgactgctc
     3241 aaaccatatt tgatctctgc aacattcctc gtcattctta cactctattt ctctaatttc
     3301 ctcattcccc atacaaacat caagcttaga gacttcgaaa aacaatatat caagaatcct
     3361 ctccgaagca aatccctcaa tcttcactta caaatcgaac ccggtgtatt cgtttacttc
     3421 gaatcatggg acaatgccag aaaaaacggt taccgcttta ccatcgaaag aatagaaaac
     3481 agtaaactca gctacaaact catgagcgat cagataaaat gggattcaat aaaatcgaaa
     3541 tggcacatca tcaattacac cgaacgcttt atcgatacag ctacctttga gcaaagcctg
     3601 gtatttggaa gccaaaagga cactactctc aatcttcgcc ccacagaaat catagtggac
     3661 aaggaagaca tgaagataat gaatttcaat gagttgaagg cgttcatcga gagagaaaaa
     3721 ctcaaaggaa gcagtacgat aaaatattac gagacggaga tgcacaaacg tatagccgaa
     3781 cctatatcgg ccatcatact tacattgatt ggtatggcac tgtcgagcag aaaagttaga
     3841 ggaggaacag gtatgcacct gggtatcggt attggcatta cttttactta tattctgttc
     3901 atgcagatca ctactcaatt ctcggtttat ggaaatatgg cgcccatgct ggctacctgg
     3961 cttcctaata tcttttttgg aattatagct atctggttat tgctcaaagc ccctaaataa
     4021 taacaggttt ttatcgacaa ctcactccca aagaataact ctttagagga aacatcggcc
     4081 aaaaaactca aatcaacttt cttcctgctg tttacggatg gaggcaatga taaacctgac
     4141 aacatccgac ttgcgttcca gcataaatcg cgcgtaatca tcttcccggt tacgaaagaa
     4201 tacacccttc aaaataggct gagcaacaaa aggaaaaata cacaaggaaa ggatattaat
     4261 gatgagctgg gttggatcgc agtttttgat tttccccgcg tcaatttctc tgtatatttg
     4321 attgataaaa aactgaggat ttagtcctgt ccctctgatc aaggcatcga tatgctggtg
     4381 gccatgagca agttcgtgaa gtataaatcc cggaatcaat ggattttcat tcaaagtgtc
     4441 gagatatttc gaaacaaact gttcgatttt cttgaacaat ggctcgtcgg agtttaaaat
     4501 caccatcacc gagggaacta ccttaataaa ggcctctaaa aaaacttcct gaaaaagttc
     4561 ttccttcgac cgaaaataat aatgaataag ggctttattt acccctgcct catcggcaat
     4621 ttcctgcatg cgggccccat cgtaaccatt ctctataaaa accttgcggg cggcttccaa
     4681 aatctcttcc cgactcgctt ttccctgtgg tttcatgttt tataccaatc aaaatttata
     4741 tcctaaagca aaaataattt ttttgaaaga agtttcctta aaaaaacaaa aaaactgccg
     4801 aatatttaaa aatttaaaac tccggcagcc ttaaaaatat acgataaaaa attatttaac
     4861 gactaataag gaatttattt cgataaacca ttccgtctgg caaatttatt acaatcatat
     4921 atatgccctg agaaagataa ggaagtgtaa tagtttgctg cctgctattg ctggataagg
     4981 tttttgtaaa aacaaccttt ccgctcatat caattacttg aagattttta ctgccatatg
     5041 aaatttcgtt ttcccattcg atgaaaagtt catgactaaa agctctgtaa gaaatcttca
     5101 aaatatccga ggcgagataa tcgattccgg cggcctgacc atcgaaggca aaatcaaccc
     5161 aaaccgaatc agcgggatga tcaaaatcat aaaatacata ggttatggtt gatgttcccg
     5221 aatgcgattt tgggtcataa tccacactca gcccttgaga tgattctcca gcagccataa
     5281 ccatgggttc gggaagttca aatacatcgg gtggataaca atctccccaa caaaaataca
     5341 tctcagtacc ctcaatcacc gacagatgaa tcttgtgcat gctcacattc ttcgtttctt
     5401 tgctgtgatt ggtcaatatc aaatcatatt gtatcacctc cgtatatgag cccacaacat
     5461 tcagaacaga agcagtagga atggaatctt ctgtgtttct cagactaagg taggaaggcg
     5521 aaaattctac acgaaacgaa acgctgtcgt tgggattatt ttccacaaaa aaagtaaact
     5581 tcagccgtgt atgacccagt acgccaccgg catcataatc ggccgtaaaa tcagtagaag
     5641 tgcctccagc atcaatcaca atgccttcgg gacttacgtt gacaaaccat gggaaacaag
     5701 ctccccagca aaaagttgca gtagaatcct tggcggcaaa ttcctcatac ttcttcacga
     5761 aaacattctt tgcctcggta gaagtattgc ttaccaaaat tctggccacc atatattcgg
     5821 tgggcaatcc accccaataa agagtagcat cattggcaat caactttcca tccaaggttt
     5881 taagctgaag actctgagct gataaccagc ctcccaaaga catcaataat acagaaagta
     5941 aaatttttct catcgcttat taatttgggt ttgtaaaatg ttggttcatg ttgcaaaaat
     6001 agagattttc atcactcggt ctaaacattc tgctgttttc ccttgattga aatatttatt
     6061 tcaacataat gaaagaacca aaacggcctt catcgccagc acgagccgag aaaaacatat
     6121 ctgaatgaca acgcgtgcaa aggttcgcca tttcgatatt gcggggattt atgccttttt
     6181 ctatcaaaat tcttcgattg gcttcccata agtcgaatac gggccgagaa aatttgggat
     6241 gattcagcaa aatgcctttg gtatcacgaa aggcttcttc cactttgctt atgacctcat
     6301 tccctacttc atagcaacat ggcccgatgg atggcccaat gcctgccaaa atatctgccg
     6361 ggtccgaatc gtaaacatta cgaaactctt cgataacttt tgcgccaatt ttcattacag
     6421 ttcctctcca accagcgtgg gcagtagcta taaccttttt tataggatca aaaaataaca
     6481 ggggaacgca atcagccatt tgaataacca gacaaatctc ctgatgttgc gttatcatgc
     6541 catcgctgcc aaggcaggtt tttaaaactt cgttgacacc cctgcccttc tcttttattg
     6601 aaatgcgctc caccttgttt ccatgaacct gtgtggtaaa cacagaacta ctcacatcga
     6661 gtccaaattt cgagaaaagg acttttcggt ttactcttac tctatctttc gacaaagaac
     6721 cattgaagga aatggaaaac tgctgatctt ttagaaacct tcgtgtggaa acaaaatgca
     6781 gaatttcccc gtcaaatctc gaaagattct cgaaataaca gcaaattggt tcatccttat
     6841 ctattaacat ctaaccctga atttctgcaa aacaaagaga ttccggacaa ttttgcaaaa
     6901 aaacatcttt gacgaagaca tttgtgctac ctttatagaa atttaataaa tgagctacga
     6961 cacgaagatt actgatttta ttaaatcaat aaatctccga cttaaccggt aggatcaaat
     7021 ggaaaggata aagtcacaaa ctaaaaaaac ccctaccgaa gcaggggcat aggactaagt
     7081 ccttcggcat atagccttgt tgtgataaga taagattgga ataatcttcg tcaacaaaga
     7141 tacaactttt ttattatgca acactttcat aaaaaatatt taattttaac ccatcttcta
     7201 aggaaaacaa aggtattaac tggacaaacc acactcagaa atctaaaatt aaatctaata
     7261 atttaaaaat taatcaatta tgagcaaaat ccttggactc gatttaggag tcaatagcat
     7321 tggatgggca gttgtagatg atgaaaaagc gtcaattatt gactgcgggg ttagaatttt
     7381 ccctgaagga gtagaaaatt taggacaggg ggaaaaagag acttcaaaaa atgcacagag
     7441 aagagaaaaa agacaactca ggagacatta ttacagaaaa agattaagta agatcaaatt
     7501 acttgagaca ttgatccctc ttaaaatgtg ccccttatca ttaaactcat tgaaaaaatg
     7561 gaaaaattgg aacaatgaat tgaaatcgga tggaagaaag tttccaaatg atcctgagtt
     7621 tatagaatgg ttgaaaatga atccttacgc attgcggaaa aaagcattag aagaagattt
     7681 aagcttagaa caactcggaa gaatctttta tcattttatt cagagaaggg gtttttacag
     7741 tagtcgaaaa ggaaaagatt caggaaagat ttatcaaggg gaaaataata ccattggtat
     7801 actcgagacc gagaaagaaa ttggttataa taaaactctt ggaactactt tgtatgaaat
     7861 gattcttccc gagcatcaga cttatatcta cagaactgat gagaatagac gtccgctaag
     7921 agcccgtaac cgatatacct taagagaaat gtatgttaag gaatttcatg ctatttggga
     7981 aaaacaagct ccacatttag gattaaatga taaaactgtt acgatattca aatcctttca
     8041 tctttctggg aataatgatt ctgaaagaaa taagcaaaga atcaataaaa tttacttgca
     8101 atatggcatt gagaatgtta ctattactgg gaaaaccata aaagttgcac aacagatctc
     8161 cttaaaaact ctttttgggg gacgaatcga aattaatgac gaaactcatg aaatattata
     8221 tagaagtcag gatagtcttt tattctggca acggccatta agatcacaaa aacatctttt
     8281 gtcaaaatgt actttcgaaa agagaataat gtttgataaa actaccaaca aatggataga
     8341 aaccgggcct accccttgtc caatttctca tcctgatttc gaactatata gagcatggca
     8401 attgataaat aatatacgat acggtcgaaa ggaacaaccg cttgatgcta gtcagcggca
     8461 aactgtttta aatgtaatat catatcagga taaagttttc gactttaaag ttattattga
     8521 tgccttaaag ctgcaaaatg aatatttcaa ttacgatgca gaccaaaaag tttctggatg
     8581 tcctactata aaaaaattaa aaagtttgtt tcctactgag atatgggtgc agaataaaca
     8641 tgaaatatgg catgatttca tgttttttga agatgaagaa cttttgaccc aacggctcat
     8701 aaaaaaatac catttcactg aagaaaaagc ccaaaaaagc gcaaaaatta cacttgaaga
     8761 tgggtatgca tcaatctcat taaaagctat tcataacata ctgcctttcc ttgaaaaggg
     8821 atttattttt caacaagccg tcttgcttgg agggatcaaa aacacttttg gcagcagatg
     8881 ggaatatttt aaagattatc atagtgatat agaacaattt atcaaagatc tttgcaatgg
     8941 caaaaaatat catgaaggcg aaattattca gaatataaaa gaataccttt cgagcactgt
     9001 taataatttt ggatttaacc ctaatgataa agccttttta aaattatacc atcccagtcg
     9061 ggaggtaggt ccagagaaaa agggagatta catttcacct gtcgagaatt taagaaatcc
     9121 acttgtccaa attgggctaa atgaaatgcg ccggctcgta aattatctac tgaaaaaata
     9181 tcgtagtatg cccgaatttg ggccagattt ctgttttgat cgcatccatg ttgaggttgg
     9241 acgcgatttg aaaaacaata aaaaaaggcg acaggaaatc agaaaagaaa atgcaattaa
     9301 tgaacaaaaa aataaggaag ctcgcgaaag aatcattgaa gcaggcttaa aaccttcacg
     9361 tgataacatc attaagtatc tactttatag tgaaatcgaa cagaaaaatg gtatcgtaat
     9421 ctgtccctat acgggtaaaa ccattcgcct ttccgatttg cttgggcctc ataatttttt
     9481 ccaaattgaa catattatac cctattccgt tagtttagac gattcattcg gaaataaaac
     9541 tctatgtgaa gccaatttca atcgttcgaa aggtgaattg acaccttatc agttttattt
     9601 aaaaaacagt agtaaatctt tatggaatgc tgattcatgg gaagaaatcg agcaaagagt
     9661 cttccaaatc ttgccctata aaaaagcaag gcgttttact tcaaaaaaac ccgctatgct
     9721 tgacgatttt attcaacgac aactcaacga cacacgttat atcagcaaaa aatcagtaca
     9781 aatcttaagg gaaatatgcg acgacgttag gtcattccct ggccaactta tagccgattt
     9841 acgccgatta tggggactaa acgatattct tagtgaacct ataaccgata ttcccttgaa
     9901 tgatttaaat ataaacgaaa gcgaaccaac tattcattat cttatcgttg atgaaaacaa
     9961 tcatgttaaa aaagccatcc caaaaataaa tttcaaaccc ataccaatgg ataatgagtt
    10021 agccatacct ggtgaaataa aaaaagagac tgggaataaa ttctattttg aatcagatag
    10081 taaatactat gcctcactaa aaattcctgc aaatggcttt gaaactggga aatattgggc
    10141 ctttataaaa gctcaggctg cacaggaatt cattccaagg tttactcctc aaccccaaat
    10201 tagcgaaaat gtaataatta ttaagggtaa tctcaagaaa aaaagattca ttaacgaaag
    10261 tcatggaatt aatttcaata cgaacaaaat aatagaagat ggatattatt gggctataat
    10321 tcctgtaatc aaagctgaaa tcaaacaaaa tgatattaat gataatagaa agaatgaaaa
    10381 taaaataagc atccaaggta gagtttcaaa tcaaaaattt atatcatcca tttattcctg
    10441 tgaagctcaa ctaatcaacg ggttttatac tgcattatta gactttgatg taaataatgc
    10501 tgatttttta ccggcactta atttacctcg tcctcctgaa aactattata cactaaatgg
    10561 aactgtggat agccagggca tattttgtcc cgatatggac tacaattgga aattaaaagc
    10621 cgatgttgca ccagggcgtt actttattta ttttaaaata caggaaatca ttaattttca
    10681 acctattgaa aatcctaagc ccattatcga taaaaataat aacgaaaaaa taattgaaag
    10741 tttgattagg gtggataccg agacgggaga aattattatt gatccgaaga aaaatcgtga
    10801 agaccagcgt catcatgcca ttgacgccat tgtaattgct tctgctacta caagttacta
    10861 tcaaaaacta tctacttatt ttggtaatct caaaaataaa caacgtaatt tggctccaaa
    10921 acccaaattc gaacaaccta acccacactt tcaaaatgct gtaaaagaag caatttcaaa
    10981 aattttggta tcttatcgaa ctaactatag agttttggtg cctgtttata agagaattcg
    11041 taaaaatcat gatacttatg tttccaaagg atattcggta aaagggcaac ttcacaaaga
    11101 tacattctat ggaggttata ttcttgacca gggagaaggt aaaatagtat cggctgctaa
    11161 atatctacca ggtattaaac cacaacaata tcatgtaaga tgtaatatta ccgaaataaa
    11221 agatcataaa cacgtagcga aaatcgttga tactactatt aagaagctaa ttgaaaaccg
    11281 acttcaaaat gtatttaata tcgatatttc aaaaccttat aatatcccaa atggcttctt
    11341 tttcgataag gataaaaatc ctgctatacg gcttccaaat cgcaaaggag gtgatcctgt
    11401 tcctgtatat acagttagaa tttttgaata taagggaaat gctgttaaat taaagccaaa
    11461 tataaattgc tatgttgatc ccagaaataa tcatcattgc attatttaca aagatgtaga
    11521 agggaatcta aaatttgaga tggtcaagtt ttgggatgct gtaaatcgct tgcgtcacaa
    11581 acagccatta tatcagcttc cgcctgatgg ttcacaaata attgccaccg tgcaggaaaa
    11641 cgatatgttc atatttaatt atctttatgc ctccgatata tccgaagata tgaaaatccc
    11701 ctatgaagag ttaagcaaat atttataccg tgtgcagaag atatcatcaa cagaaggagg
    11761 aattttcctg acattcaggc atcacttggc ttccactatt aagaatcccg aggaagaatt
    11821 aagaatacaa agtttaagaa gttgggaaag gcaccaaccc ataaaagtaa aaataaatat
    11881 ggatggtact gttaataaga ttattaaatg ataaagcgca ctctttgcat acagaatgca
    11941 gggcatttaa acttgaataa taatcaatta gtttttaccg ataaggaagg aattaaaaaa
    12001 actgcaccta tcgaagacat aggctatttg ataatcgaaa atcgttatgt aacgataagc
    12061 gtgcaactta ttacggcttt aaattcgaat aacgcggcaa ttattttttg cgacgaaaaa
    12121 tatttgccta ccggcatgtt gctaaacctt gacagtaatc aggtccaaac cgaaattttt
    12181 catactcaga tagaagcttc cgaacctcta aaaaaaaatt tatggaaaaa tgtcattact
    12241 tgcaaaatcc gaaaccaggc tgccttatta cataaattaa ataaaaatga tcagccttta
    12301 aagatttttg ccaatgaagt tatgagtggc gatacgagca atagggaagc tttagcggca
    12361 aaatattact ggcatgagct gtggggatcg ggttttatac gcgaccgttt tgggccacca
    12421 cccaattcat tgctaaatta tggttacatt atcattcgtt cagcaatggc tcgagcattg
    12481 gctggttccg gccttctttc aactcttgga atattccata gcaatcgcta taacgcattt
    12541 tgtcttgccg acgatatgat ggaaccctat cgagtatatg tagatgaatt ggtttatgag
    12601 gtttggtata aaacagataa acctgaaccc gataaagaat ttaaacagat ggctattcaa
    12661 ttgttagcac gagatgtaaa tttggaaaag actacgaggc cgcttatgtt agccatgcag
    12721 caatcgagtg cctcacttgc cagatgctat ggtggaaaac aaaggaagct gtcattccct
    12781 gattttaaat gatatacatt ttagagatat tatttgaacg acttaatgca tatcaggtta
    12841 tgtggttatt tgtatttttc gatttacccg taggaactaa aaaagaaata aaagaagcta
    12901 cacgctttcg caaaaatctg gaaaaagatg gcttcaatat gatgcaatat tcggtatata
    12961 cacggcactg cccaagccgt gagaatgccg aggttcatgt aaagcgggtg aaaagcataa
    13021 tcccggtaaa aggtcaagta agtatcttga tggttaccga caagcaatat ggtgaaattt
    13081 ataatttttt cggtaaaacg gaaaaatttt tacctgaagg accaaggcaa cttgaatttt
    13141 tttaattttg ccaaacgttc attaacaaaa ctgaaaaacc aaaggttgat aaatgttttc
    13201 ttaagagttt atgcaatttt tttatcctaa attgatcgga aaagccttga ttttacttgg
    13261 tttttggcta agtctgttgt ggtttgataa gaaattggaa tagaagacat tgtgtaacgc
    13321 attgtgttgc aggaaattga tgttgtggtt tgataagaaa ttggaataga agacatttct
    13381 tatgctgata cacagatgaa tcataacgtt gtggtttgat aagaaattgg aatagaagac
    13441 attagttgtt ggatattttg ggataagggg acagttgtgg tttgataaga nnnnnnnnnn
    13501 acattaaagt ttaaaacttc tatttcttca tctgtgttgt ggtttgataa gaaattggaa
    13561 tagaagacat tatttcgcaa acaggtgatt ttatcggagg agttgtggtt tgataagaaa
    13621 ttggaataga agacatttag aaggcttgaa ttactattac cccgtcagtt gtggtttgat
    13681 aagaaattgg aatagaagac attgccaccc tcgtcaacta cctttatcct aa
//
LOCUS       DAOORU010000049        13694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_5501_length_13694_cov_6.112618, whole genome shotgun sequence.
ACCESSION   DAOORU010000049 DAOORU010000000
VERSION     DAOORU010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5501_length_13694_cov_6.112618"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..710
                     /gene="bcp"
                     /locus_tag="PLF20_04630"
     CDS             255..710
                     /gene="bcp"
                     /locus_tag="PLF20_04630"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HOX80325.1"
                     /translation="MLKVGDKAPDFLGIDQNENTISLKDYEGKKVVLYFYPKANTPGC
                     TAEACNLNDNLERLKAKGYEIIGVSADSVKTQKSFAEKYHLNFPLIADTDKNIIKSYG
                     AWGKKKMYGKEYEGIMRYTFLISEDGIIQDIITQIDTKHHSSQILEKDF"
     gene            1208..2215
                     /gene="recA"
                     /locus_tag="PLF20_04635"
     CDS             1208..2215
                     /gene="recA"
                     /locus_tag="PLF20_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007900567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOX80326.1"
                     /translation="MDNTDKQAKLKALEATISNIEKNYGKGAIMRLGDQPTEAIPVIS
                     TGSIGLDTALGVGGLPKGRVIEIFGPESSGKTTLALHAIAEAQKQGGIAAFIDAEHAF
                     DRTYAQKLGVDTDNLLVSQPDYGEQALEISDNLIRSNAIDIIVIDSVAALTPKSEIEG
                     EMGDSKMGLQARLMSQALRKLTANISKTKTCCIFINQLREKIGVMFGNPETTTGGNAL
                     KFYASVRLDIRRVTQLKEGENITGNRVRVKVVKNKVAPPFRQAEFDIIYGEGVSKLGE
                     IIDLGSELGVIKKSGSWFSYGDTRLGQGRETVRQLLVDNPELAEELEKNIRTKLSEVV
                     L"
     gene            2960..3466
                     /locus_tag="PLF20_04640"
     CDS             2960..3466
                     /locus_tag="PLF20_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80327.1"
                     /translation="MKEIRSVKANPHRSSMFSSFPFGRFARGYLLLTFVMAGIVSFTS
                     CRVQLIPNRDEAVITQTLQIAKSIDSFYLNLMNTDSSDLAYANFSDSYQAIELELRSL
                     WLQSEAKPLNSESARISKTVLDLWVKYKEQHRKNNTYNLHLAPIHRDRFHELFVAFLN
                     AEKAKTIQ"
     gene            3478..3792
                     /locus_tag="PLF20_04645"
     CDS             3478..3792
                     /locus_tag="PLF20_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80328.1"
                     /translation="MDLNFEDLIEGLSSILKNELEEGKEDVKTYARYIIEKRKQRLEQ
                     LAELYTRGFITKEELESELADEEKVIEAHLLSMQVMAKASIQQAANASIQFITDYLLK
                     LL"
     gene            3804..4571
                     /locus_tag="PLF20_04650"
     CDS             3804..4571
                     /locus_tag="PLF20_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80329.1"
                     /translation="MENNNGNSPTKDYMSARNVIAFLITLVILVATVYVAIVAVRISS
                     EQDRMEGLKFVAQTLLPLWGTWIGTILAFYFSRENFDAATKSYQNIIESMKPEEKIAK
                     IMAKDAMLPLEKITYLDYDETKTRTIRDILDDERFREYNRYAILNRDKTLKYIIHRST
                     FYRFLAEVSMGIVPIEKNTDDLTFSDMEEQASDAVKAMMYKGFNFIAENSTLLDAKKA
                     MDAIPECQDVFVTKSGKATEPILGLITNNKILDYARV"
     gene            4584..5258
                     /locus_tag="PLF20_04655"
     CDS             4584..5258
                     /locus_tag="PLF20_04655"
                     /inference="COORDINATES: protein motif:HMM:NF013534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016977 - chitosanase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitosanase"
                     /protein_id="HOX80330.1"
                     /translation="MITPEIKRKILQVVNVFETGKLEGRYDALVVMNDGPGGIPQITF
                     GRSQTTEYSGLRDLILRYIKREGVYAAQLSEYEPRIGKTPLTNDQVFKQLLIQAGRND
                     PLMISVQDEFFDQTYYQPAFKFFTTNGFELPLSLLVIYDSYIHSGRVPDFLRRRFGEK
                     IPARGGNEKEWVTRYCDVRHQWLKYHSNPILRKTIYRTACFKEQIALGNWMLVLPIRV
                     QGLVAV"
     gene            complement(5230..5892)
                     /gene="nth"
                     /locus_tag="PLF20_04660"
     CDS             complement(5230..5892)
                     /gene="nth"
                     /locus_tag="PLF20_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOX80331.1"
                     /translation="MTTKERYKRFIAWFSENMPEASTEPVYQNPYQLIVAVILSAQCT
                     DKRVNQITPAFFEKFPNAISLATASHEEVLALIKSCSYPNNKTKNLIGMAQTLLEKFS
                     GQVPDNLDDLESLPGVGRKTANVILSVAFNKPAMPVDTHVHRVARRIGLVKDARTPLE
                     TEKQLTRYIPAHYLSKAHHWLILHGRYVCQARKPKCDICGIQDICLYYQNIIKPPQGP
                     EP"
     gene            complement(5965..6306)
                     /gene="rplT"
                     /locus_tag="PLF20_04665"
     CDS             complement(5965..6306)
                     /gene="rplT"
                     /locus_tag="PLF20_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOX80332.1"
                     /translation="MPRSVNVVTSHRKRRKFLRRTRGQFGRRKNVWTVARNSWEKGQL
                     YAYRDRRAKKRSFRALWITRINAAVRQYGMSYSEFMGKLDKANIRLNRKVLADLAMNH
                     PDAFKALVDQL"
     gene            complement(6389..6583)
                     /gene="rpmI"
                     /locus_tag="PLF20_04670"
     CDS             complement(6389..6583)
                     /gene="rpmI"
                     /locus_tag="PLF20_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOX80333.1"
                     /translation="MPKMKSKSSAKKRFTYTGTGKIKRKHAFKSHILTKKDTKRKRNL
                     THSTLVSKADYSTVKKMLAC"
     gene            complement(6633..7190)
                     /gene="infC"
                     /locus_tag="PLF20_04675"
     CDS             complement(6633..7190)
                     /gene="infC"
                     /locus_tag="PLF20_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOX80334.1"
                     /translation="MAALQKRPEKNPAAKNQEEPYRVNEKIKAPIVRVVGDNFEQRIV
                     TIKEALEIADQMELDLVEISPNANPPVCKLIDYSKFMYELKRKQKEAKAKAAKVILKE
                     IRLGPNTDEHDFNFKLNHARKFLEEGAKVKVEVLFKGRTIVYKEKGEIILLEFAQALE
                     DIGKLESLPKLEGKRMFITIAPKKS"
     gene            complement(7254..9185)
                     /gene="thrS"
                     /locus_tag="PLF20_04680"
     CDS             complement(7254..9185)
                     /gene="thrS"
                     /locus_tag="PLF20_04680"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOX80335.1"
                     /translation="MINIKFPDQSIRQYPEGVTGLEIARSINEGLARKVLAIKLNGEV
                     WDVTRPIFQDASIRLLTWDDDEGKATMWHSSAHLMAEAIESLYPGVKFGVGPDIENGF
                     YYDIDFGDYQISSDDFKKIEDRMLELARQKQEFKRRDVSKAEALEYFRRKGDEYKIEL
                     INDLEDGSITLYETGQFTDLCRGPHLPNTSPIKAVKLLSIAGAYWRGDEKRKMLTRLY
                     GITFPKAEMLAEFLNMLEEAKKRDHRKIGKELDLFHFSSRVGNGLPLWTPRGALLRER
                     LTDFLKKELYKRGYVLVASPHIGNVNLYKTSGHYEKYGADSFRPISTPEPGEEFMLKP
                     MNCPHHCEIYNRKPMSYKELPQRYAEFGTVYRYEQSGVLHGLTRVRGFTQDDAHIFCR
                     PDQLKEEFIGVIDLVLFLFKTFDFNNFIAQVSLRDPENKIKYIGSDENWDRAEQAIIE
                     AAEEKNLNPVLVKGEAAFYGPKLDFIVKDAIGRKWQLSTIQVDYNLPERFELEYIGAD
                     NQRHRPIMIHRAIFGSIERFVAVMLEHTAGKFPLWLAPTQAIILPVSEKFHNYAENIL
                     QLLKNCEIRSLIDDRSEKIGKKIRDAELQRIPYMLIVGEKEENNNTVSVRKQGQGDMG
                     SFKVQEFVDAVEIEVQNFK"
     gene            9713..10033
                     /locus_tag="PLF20_04685"
     CDS             9713..10033
                     /locus_tag="PLF20_04685"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOX80336.1"
                     /translation="MEDVKMVDFFKNAILAGIGLVSLTREKAEEFAKELISRGELSEN
                     EKAKFIKDVMEQTEKSKTELEKKIEKSIENALSKLNIPSRKEFEELKKKVEELSQTSA
                     KKEE"
     gene            10072..11769
                     /locus_tag="PLF20_04690"
     CDS             10072..11769
                     /locus_tag="PLF20_04690"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HOX80337.1"
                     /translation="MAEPYSYVNFRFRSNFDKVRRSRQIISVFFKFGLDYFLGALRVN
                     IFTRLRKRKKGYKKLSNAERLRMAFEELGPTFIKFGQILSTRPDFLPPDYIRELEKLQ
                     DTVAPIEVKKIQEIIETELHRPIAQAFKEFDEQPVASASLSQVHRAVLQNGNVVAVKV
                     QKPDIQNTIELDISILETFANIIEKRFPEGPFYHPMAMVNELKRTIRKELDFLNEAHN
                     FEKFRTNFKDVNCIKIPEVYWDLTTERLLVMEFIDGIKISEITHEKYRSRFDLKKVAH
                     QAAEALLKQILLDGFFHADPHPGNLFVIPPNTIVMLDVGMTGHLDDQSLLSIAKILRA
                     INHRDFDQIIRGLQELGIVMQEVDKTLLRHDIKELLDIYTDRPFKTISLARMNQDILD
                     IAMRHRLAIPSNFVMMIRAMSIAETIGKQLESDFDILEMVKPFTRKIYLKLYSPRSWK
                     KRTANMLDASVELIEHLPQNIYDLLEKLKEGQFEIHLEHHRLENLANEIKSSSNRIAV
                     SLIIAALIVGSSILIHQKIGPMVSNVSVWGIIGFVIAIIMGIGLLISSFRKNRRRKF"
     gene            complement(12400..>13694)
                     /locus_tag="PLF20_04695"
     CDS             complement(12400..>13694)
                     /locus_tag="PLF20_04695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80338.1"
                     /translation="ATGSVVYQGNIIVFRGLWDDVAKDVNYEIQTVELYTESNNRPAN
                     QRVAISPDGQTAWIVALANNENLTTPGMLNYYPILFKSTDAGQTWQGPIEVQLDGPNG
                     IKEIVYGLLSDSMMAVLYNPPLPSREEIGYTTAFDCDIVVDKFGNPHIGVIVGVTGNS
                     DYSIVVAEKSFAAFDIYSIDGGINWYAQKMGYPQQFRGNWGDNSEDNRTQIASTMDGT
                     KVFVTWLDTDPTFSDNNNSPNVFCRGFDLMANKTTGTLTGGKYVDLPNNVTLFSDVYN
                     ESFFACLSRYVFTDNNKYTIPIVIEGLSDVTSMDSWVQFKYIPDFYYTDADFVFSSNN
                     PGVGIPQLNMLDVSLSEAYPTPAENQVYFSLTLDKSATVSYDIFTVSGQKLTSQIYGR
                     VAQGIQTLQVDISSLKTGIYFIRFNVNGNTFTRKLVVR"
BASE COUNT         4089 a         2490 c         2944 g         4171 t
ORIGIN      
        1 tctcggtggg cctcgatcac gacattgcag aaacctggtc gaaaatcgaa acctgcctca
       61 aagaacttcg aatcatctga gatgtatttg ccctatgcgg tttcagtttt tatatggaaa
      121 gttcataatc gcattttcaa cgcatttcac cccatgagca atgacttttt gaacaaataa
      181 ggatcaaatt atgttatttt cagtaaaagt cataatattt catccaaatt caaccattta
      241 aaatcaaaat agttatgcta aaagttggag ataaagcccc ggattttttg ggcatagatc
      301 aaaatgagaa tacaatttcc ttaaaagatt acgaaggaaa gaaagtagtg ctttactttt
      361 accccaaagc caatactcct ggttgtacag ccgaggcctg caatctgaac gacaatttag
      421 aacgattaaa agccaaaggt tatgagatta ttggcgtaag tgccgatagc gtgaaaaccc
      481 aaaagagttt tgccgaaaaa tatcatctga acttcccttt gatagctgat accgacaaga
      541 atatcatcaa aagttatggg gcatggggca aaaagaagat gtacggaaaa gaatatgaag
      601 gcattatgcg ttatactttc ctgatttcgg aagatggaat tattcaagat attattacac
      661 aaatcgatac taaacatcac agctcacaga ttcttgaaaa agatttttaa aatattgcta
      721 tatggacaaa aagctaatct ttcccatttt tgaatagaaa ttccaagcaa aaaaaggtcg
      781 ttgcttcgag acatatcatc ctcttacatt aaaggcctgg ctgacagcta ttacattcct
      841 catatcgaca agaagcccaa tcttcatgga gattagcaac aaagacagtt attctatgac
      901 acatccggtg acgatattat cctgactttc gaagacggcc tggtcctgag tggtagtaat
      961 gattagacga cttagtaatg aaaatagccg tcgaatcttc catagcagtc aatttatcgc
     1021 tgtatactgt ttgaaagtct tttttagaaa atccaacggg gagaatatga catttctcag
     1081 tgtaagctaa attttaatga ctcgggtttt tattgtagtt cgattatcga aatttatttt
     1141 acctttgatg gacttatagg gaaaaacgat aagaagaaaa ttactcaaat aattcaatca
     1201 catcatcatg gataataccg ataaacaagc aaaattaaag gctctggaag ccactatcag
     1261 taatattgaa aagaattatg gcaagggagc cattatgcgt ttgggggatc agcccaccga
     1321 ggccattcct gttatctcga cgggttccat tggcctggat actgctcttg gtgttggggg
     1381 tttacccaag gggagagtaa tagaaatatt tggtcctgaa tcttcaggta aaaccacgct
     1441 ggcgcttcat gctattgctg aagctcaaaa acagggaggt atagctgctt ttatagatgc
     1501 cgagcatgct ttcgaccgta cgtatgctca aaaactaggg gtagataccg ataaccttct
     1561 ggtttcacag cccgattatg gagaacaagc cctcgaaatc agcgataacc tcatcagaag
     1621 caatgccata gatatcatcg tgatcgactc cgtagcggcc cttacgccta aaagcgaaat
     1681 agaaggcgaa atgggagatt caaaaatggg ccttcaagct cgacttatgt cgcaagcttt
     1741 gcgtaaactt actgccaata tcagcaaaac taagacttgc tgcatattta tcaaccaatt
     1801 acgcgaaaaa atcggtgtca tgtttggtaa tcccgaaact accacaggag gtaatgcatt
     1861 gaaattttat gccagtgtac gactcgacat tcgacgcgtc actcaattga aagaaggcga
     1921 aaacattacg ggcaatcgcg tcagggtgaa agtggtcaag aacaaagtgg caccgccttt
     1981 tcgtcaagcc gagttcgata tcatctatgg agaaggcgtt tccaaactcg gagaaattat
     2041 cgatttgggg tctgaattgg gtgtaattaa aaaaagtggc tcatggttca gttacggtga
     2101 tactcgccta ggccagggca gagaaacagt aagacaatta ctcgtagata atcccgaact
     2161 ggccgaagaa ctcgaaaaaa atatcagaac caaactgagt gaagtggtat tataacttcc
     2221 tggcttagcc ccctattgtt aagactatat tagtaaggat ttaaagagct aaagcatcat
     2281 cttggccgtt tttgacctga tttttaaaat tgaatcaaga aataattaga actatccttt
     2341 gcaaaactta taacttggcg tactttgagg ttaaattgcg tttagagttg gatggtgatt
     2401 ggtgagtaaa aagttgaaag ttaaaagttc ataaagttaa aaagggagga agggagaaaa
     2461 ggaaaaatgg aagaatggag gaagggagaa tggtggatgg tggatggtgg atggtggatg
     2521 gtggatagtg gatggtggat agtggatggt ggatagtgga tagtggatag tggatagtgg
     2581 atagtggatg gtggatggtg attaggagac tgggagactg aaatttggag tttggaattt
     2641 gtaatttgaa atgttgtagt tgtaagttgt tcgttgtaag tggttttaca aagagatggt
     2701 taacctatct ttggaacatc acctcattag tttcgattag aattctttgc agactaaatc
     2761 gaatgattgg ggttttgtta taaatttttc acaacccatt aaaaattcga ttctctatac
     2821 atttttcttt tttatatttg gcgttgtatt tcattctatt tgaaatcata tcatcatcaa
     2881 tgaattcaaa aaggctgaat aatattttac gcgcccatca accgaacatc caaggatttt
     2941 taaaataaat tcgtgcaaca tgaaagaaat tcgttctgta aaagccaatc ctcatcgttc
     3001 gagtatgttt tcgtcttttc cattcgggcg gtttgctcgg ggttatctgc tcttaacctt
     3061 tgttatggcg ggaattgttt cttttacttc gtgccgggtt caactcatcc ccaaccgaga
     3121 cgaagccgtg ataactcaaa cgcttcaaat tgctaaatct atcgattcat tttatttaaa
     3181 cttgatgaat acagattcat ccgatcttgc ttatgctaat ttttctgatt cttatcaggc
     3241 tatcgagctc gagctgcgtt cgttgtggtt acagtcggag gccaagcctt tgaatagcga
     3301 gtcggctcgc atatccaaga ccgttctcga tctctgggtg aaatataagg agcaacatcg
     3361 aaaaaataat acttataacc tgcatcttgc tcctatccat cgcgacagat ttcacgaact
     3421 ctttgttgcc tttctgaatg ccgaaaaagc caaaaccatt caataaattt ttttgctatg
     3481 gacttaaatt ttgaagattt gatcgaggga ctttcatcca ttttgaaaaa tgaattggaa
     3541 gaaggaaagg aagatgtgaa aacttatgcc cgatatatta tagaaaaacg gaaacaacgt
     3601 cttgagcagc ttgccgaact ttatactcga ggttttatta ccaaggaaga attggaatcg
     3661 gaattagccg acgaagagaa ggtaattgag gctcatttac tgtcaatgca ggttatggcc
     3721 aaagcatcca tacagcaagc cgccaatgct tccatacaat ttattacaga ttatttattg
     3781 aaactcttat aaatagatta actatggaaa ataataatgg caacagtcca actaaagatt
     3841 acatgagtgc aaggaatgtc attgcttttt tgattaccct tgtgattctc gtggccacgg
     3901 tttatgtggc tattgtagcc gttcgcatct cgagtgagca ggacaggatg gagggattga
     3961 aatttgtggc tcaaactctt ttgccgcttt ggggaacatg gataggtacc attctggctt
     4021 tttacttcag tcgcgaaaat ttcgacgctg ctacaaaatc ttatcaaaat atcatcgaga
     4081 gcatgaaacc cgaagaaaaa atagccaaaa tcatggctaa ggatgccatg cttccactcg
     4141 aaaaaattac ctaccttgat tatgacgaaa ccaaaactcg tacaatacgc gatattctcg
     4201 atgatgagag gttcagggaa tataatcgtt atgctatact caatcgagat aagacattga
     4261 aatatattat ccatcgcagc actttctatc gatttctggc tgaggtctcg atgggtatag
     4321 tcccaatcga gaaaaatacc gacgacctca ctttcagtga tatggaggaa caagcaagcg
     4381 atgccgtgaa agccatgatg tacaagggat ttaatttcat tgcggagaac agtaccttgc
     4441 ttgatgccaa aaaggccatg gatgccattc cagaatgcca ggatgtgttc gtaaccaaaa
     4501 gtggcaaagc cacagaacct attctgggat tgattaccaa caacaagatt ctcgattatg
     4561 ctcgtgtgta aatccttaga aaaatgatta ctcccgaaat caagcgcaaa attttgcagg
     4621 ttgtaaacgt tttcgaaacc ggaaagttag aaggacgtta tgatgcccta gtagtgatga
     4681 acgatggtcc aggaggaatt cctcagatta cttttggtcg cagccagacc acagaatata
     4741 gtggactaag agaccttatc ctgaggtata tcaaaagaga aggtgtatat gcagctcaat
     4801 tgagtgagta cgaaccacga attgggaaaa caccgcttac gaacgatcaa gtttttaaac
     4861 aattgcttat acaagccggg cgcaatgatc ctttgatgat cagtgttcag gatgaatttt
     4921 tcgaccagac atattatcag ccagccttca agttttttac taccaatgga tttgagcttc
     4981 ccttgagttt actggttatt tacgatagtt atattcattc tggacgggta cctgactttt
     5041 tgaggcgtag atttggtgaa aagattccag cacggggagg caacgagaaa gaatgggtta
     5101 cacgatattg cgatgtcagg catcaatggt tgaagtatca tagcaatccc atcttacgaa
     5161 agacgatata ccgtacggcc tgttttaagg agcagattgc cttgggcaac tggatgcttg
     5221 tgctgcctat cagggttcag ggccttgtgg cggtttgatg atgttttgat aatacaggca
     5281 aatatcttgt attccgcaaa tatcgcattt gggcttacgt gcctggcata catatcttcc
     5341 atgtaaaata agccagtgat gagcttttga gagataatga gctggaatgt agcgagttaa
     5401 ttgtttctcg gtttccaagg gagttcgggc atcttttacg aggccaattc ttcttgcaac
     5461 tctatgaaca tgtgtatcga cgggcatggc cggcttgttg aatgcaaccg aaagaatcac
     5521 attggccgtt tttctgccaa caccaggcaa actctccaaa tcatccagat tatcgggcac
     5581 ctgaccactg aatttttcaa gcagcgtctg tgccatgcca atcaagttct tagttttatt
     5641 attgggatag gagcatgatt ttatcaaggc taagacttct tcatgggatg ctgtggccaa
     5701 actgatggca ttgggaaatt tttcaaaaaa ggccggggta atctggttaa cgcgcttatc
     5761 ggtgcattgg gctgaaagaa taactgctac aatcaactgg tagggatttt gatataccgg
     5821 ttcagtcgaa gcttctggca tattttccga aaaccatgca atgaatcttt tgtagcgttc
     5881 tttggttgtc atactgaata agtaaaaaga aagcggcgct tgtaatacat caagggccgc
     5941 tttcaggttt aaggtaattg ctgcctataa ttgatcgacc aatgctttaa aggcgtcggg
     6001 atgattcatg gcaaggtcgg ccaatacttt tctgttgagg cgaatattgg ctttatcgag
     6061 tttacccata aattcggaat aagacattcc atactggcgt acggctgcat tgatacgagt
     6121 aatccataat gccctgaaag agcgtttctt ggcacgtcgg tcgcggtaag catacaattg
     6181 gcctttctcc catgagttcc ttgcaacggt ccacacattt ttacgacggc caaattgacc
     6241 acgagttctt ctgaggaact tccttctttt tctgtgtgat gtaactacat taactgatct
     6301 tggcattttt ttgttttttt tacgtcagcg ttctgacggt ttcagaatct tgatgagctg
     6361 aacgtaaggt tagacataaa gtaaattatt agcaagcgag catctttttt actgttgagt
     6421 aatcggcttt ggataccaga gtggaatgag taagattacg ttttcgcttg gtgtcctttt
     6481 tagtcaggat atgactctta aaagcatgtt tgcgcttgat ttttcctgtt ccagtgtaag
     6541 taaaacgttt cttagcactg gatttcgatt tcattttagg catcttttga cttttattat
     6601 tatgaacggt ttttttattt atttcttatt ctttaagatt ttttaggtgc tatggtgata
     6661 aacattcgtt ttccttcgag ttttggaagg ctctcgagtt tgccaatatc ttcgagtgct
     6721 tgggcaaatt cgagaaggat aatttctcct ttttccttat aaacgatggt tctaccctta
     6781 aaaagaactt ctacctttac ttttgcgcct tcttcgagga atttacgggc atgattgagt
     6841 ttaaagttga aatcatgctc gtcggtatta ggtcctaaac gtatttcctt taatattacc
     6901 ttggccgctt ttgcctttgc ctctttttgc ttgcgtttga gttcatacat aaatttactg
     6961 taatcgatga gtttgcaaac aggtgggttg gcattgggtg aaatttctac cagatccagc
     7021 tccatttgat cggctatttc gagggcttcc tttatggtaa cgattctttg ttcgaaatta
     7081 tcacctacta cccgaacaat gggcgctttt atcttttcgt ttaccctata gggttcttcc
     7141 tgattttttg cggccgggtt cttttcaggt cgcttttgta atgcagctat aaatcttcct
     7201 cctttttgat ttggttaaac atatatatga ttttttggca gcatttctct gccttatttg
     7261 aaattctgca cttctatttc cacagcatca acgaattctt gtactttaaa gcttcccatg
     7321 tcgccttgtc cttgttttct cactgatact gtgttgttgt tttcttcttt ttcaccaacg
     7381 ataagcatat atggaattcg ttgtaattca gcatctcgta tttttttccc gattttttca
     7441 cttctatcgt caatgaggct gcgaatttcg caatttttta ataattgtaa aatattttct
     7501 gcataattat gaaatttttc actcactgga agaatgatgg cctgtgtggg agctagccat
     7561 aaaggaaatt tcccggctgt gtgctccagc atcacggcta caaaacgttc gatcgaacca
     7621 aaaatggctc tatgaatcat aatagggcga tgccgttggt tatcggcacc aatatattcc
     7681 aattcgaaac gttcgggtaa gttataatcc acttgtatcg tactaagctg ccattttcgc
     7741 ccaatggcat cctttacaat aaaatcgagt tttgggccgt aaaaagctgc ctcacctttt
     7801 accaagacag ggttcaaatt tttctcttcg gcggcttcta tgatggcttg ttcggctcta
     7861 tcccagtttt catccgatcc aatgtactta atcttattct cagggtctct caacgaaacc
     7921 tgcgcaatga agttattgaa atcgaaagtt ttgaaaagaa aaagaacaag atcgatcacc
     7981 ccgataaatt cttctttcaa ttggtcggga cggcaaaaaa tatgtgcatc atcctgagta
     8041 aagcctctta ctctggttaa tccatgcaaa acacctgact gttcgtaacg ataaaccgtt
     8101 ccaaactcgg catatctttg aggcaattct ttataagaca ttggtttacg attgtagatt
     8161 tcgcaatgat gtggacagtt catgggtttc agcataaact cttcaccagg ttcgggagta
     8221 gaaatgggtc ggaaggaatc ggcaccgtat ttttcataat ggcctgaagt tttataaagg
     8281 ttgacattgc caatgtgagg tgaagccact aagacataac ctcttttata gagttccttt
     8341 ttcagaaaat cggtgagtcg ttcgcgcagt aaagctccgc gcggcgtcca aaggggcaaa
     8401 ccattaccca cgcgtgaaga gaaatgaaat aaatcgagct cttttccaat tttacgatga
     8461 tctctttttt ttgcttcttc gagcatattg aggaattcag caagcatctc ggctttcgga
     8521 aaggtaatgc catagagacg cgtaagcatt ttgcgttttt catctcctct ccagtaagca
     8581 ccggctatcg ataataattt taccgccttg atgggcgagg tattgggcaa atgtggccca
     8641 cggcacaaat cagtaaattg acctgtttcg tataaagtga tgctaccatc ttccaaatca
     8701 ttgattagtt cgatcttata ctcatcgcct ttgcgtcgaa aatattccag cgcctcggct
     8761 ttactcacat ctcttctttt gaattcctgc ttttgacgtg caagttctag catgcgatct
     8821 tcgattttct tgaagtcgtc tgatgaaatc tggtaatcac caaaatcgat atcataatag
     8881 aaaccattct ctatatcggg accaactccg aattttacac caggataaag gctttcgatg
     8941 gcttctgcca ttaaatgcgc cgatgaatgc cacattgtgg ctttgccttc atcatcatcc
     9001 catgtgagta aacgaatgct ggcatcttga aatataggcc gcgtaacatc ccaaacttca
     9061 ccgtttaact tgatggcaag caccttccgc gctaaacctt catttatgct tctggctatt
     9121 tctaatcctg taactccttc tggatattgc cgaatacttt gatcgggaaa ttttatgtta
     9181 atcattatca aataattgtg ataaaacaaa actgatctca ttgggtcaat tttgggcgtg
     9241 caaagttagg ttttctcatc tgatctttaa aatcatttca tgtcgataat ttaaagtgta
     9301 tcaatgtcga acataaagga taagaaaaga tgtattttac tccattttca aagagtaaat
     9361 cctttcattt ttttcttaaa taatgagtag ctcactagat ccgtataaag agatataggt
     9421 ctggcgaacc atagtagaac attaatctgc aatgttttaa attttaattt ttctttctga
     9481 gccataactc tatgattttg tcaaaagagc attatctttg taaaaaatgt ttcagaggct
     9541 gaattcgata ttttcactaa aaattctaat caggaacaac atagatagac caagaaacat
     9601 aaaattccat aaaaatcgga ttttcggatc aggagcttga agggtgaata attgaatttt
     9661 agtattacat atatcgatca gtcaaagaat caagagtttt tgaacttaaa atatggagga
     9721 tgtaaaaatg gtagactttt ttaaaaatgc gattttagct gggatagggt tagtttcgct
     9781 gactcgcgaa aaggccgaag aatttgccaa ggaattgatc agccgtggtg aattatcgga
     9841 gaatgaaaaa gcaaaattca taaaagacgt tatggagcaa accgaaaaat cgaaaaccga
     9901 actcgagaag aagatcgaaa aatcaatcga gaatgcctta tcgaagttga atattccatc
     9961 gagaaaggaa tttgaggaac tgaagaagaa ggtggaggag ttatcgcaga catcagcaaa
    10021 aaaggaggaa tgattttttc ttttgagaat tgaaatttta aagccaattc aatggccgaa
    10081 ccttattcgt atgtaaattt ccggtttcga agcaatttcg ataaagtcag gcgaagccgt
    10141 cagattattt ctgtcttctt caaatttggg ctggattatt ttttgggtgc cttgagggtt
    10201 aatattttta cccgcttaag gaaacggaaa aagggatata aaaaattatc gaatgccgaa
    10261 agacttcgta tggcttttga agaattaggg cctactttta tcaagtttgg tcaaatattg
    10321 agtacccgtc ccgatttttt acctcccgac tatatccgtg agttggaaaa gcttcaggat
    10381 actgtggcgc cgatagaagt aaaaaaaatt caagaaatta tagaaactga gcttcatcga
    10441 ccaattgctc aggcattcaa ggaattcgac gagcaacccg ttgcttcggc ttcattaagt
    10501 caggtccata gagccgtatt acagaatggg aatgttgttg cggtaaaagt ccaaaaacct
    10561 gatattcaaa atactattga actggatatt tctattttgg aaacttttgc caacataatc
    10621 gagaaacgtt tcccggaagg tccattttat catcccatgg ctatggtaaa tgaattgaaa
    10681 agaaccataa gaaaagaact cgattttttg aatgaggcac ataatttcga aaaattcagg
    10741 actaatttca aagatgtcaa ttgtataaaa attccagaag tttattggga tttaactacc
    10801 gaacgccttc ttgtgatgga attcatcgat gggataaaga tttcggagat tacccatgaa
    10861 aagtatcgct ctcgcttcga tttgaaaaaa gtggctcatc aggcggcaga ggctttattg
    10921 aaacaaattc tgttggatgg atttttccat gccgatccgc atcccggtaa cctcttcgtg
    10981 attccaccca atactattgt gatgctggat gttggcatga cggggcattt ggatgatcaa
    11041 tcactattga gtattgctaa aattttacga gccatcaatc atagagattt tgaccaaatt
    11101 attagaggtt tacaggaatt gggaattgta atgcaggaag tggataaaac cttgcttcgg
    11161 catgatatta aagaacttct ggatatttat accgaccgac ctttcaagac aatcagcctg
    11221 gcacgtatga atcaggatat cttggatatt gcaatgcggc atcgtttggc tataccttcc
    11281 aacttcgtga tgatgatcag agccatgtcg attgccgaaa ccattggcaa gcagttagaa
    11341 tctgatttcg acatcctgga gatggtgaag ccatttacta ggaaaattta tcttaaactc
    11401 tattcccccc gaagctggaa aaaacgtact gccaatatgt tggatgccag tgtcgaactc
    11461 attgaacatt taccacaaaa tatctatgat ctgttggaga agttgaaaga aggacaattc
    11521 gagattcatt tggagcatca ccgcttagaa aaccttgcca acgagattaa aagctcgagc
    11581 aaccgtattg cagtgagttt gattattgca gccttaatcg ttggctcttc aatcctcatt
    11641 catcaaaaaa taggaccaat ggtttcgaat gtttcagttt ggggaattat aggatttgtg
    11701 atagctatta taatggggat aggattgttg atttcttcct ttagaaaaaa tcgaagaaga
    11761 aaattctaag agtgattcat agaaaaatga aggatgtgag acaaggtgga tggtgattag
    11821 taatttgtga ttggtggatg gatgagttaa aagttgaaag tttataaagg aaaaagggag
    11881 gaagggtgac gtggggacga ggagactggg agacgaggag acgaggggac tggtggatag
    11941 tggagtgtgt cgatggggat gagttaaaag ttcataaagt taaaaacgga ggaaggagga
    12001 atggagataa ggggactggg agacggagag acaagagaac tgagagatga ggggacgaag
    12061 agacgaggtc aattggtgga tggtggatag tgattggttg gggattgtag atagcagtta
    12121 gtgattgaag cttgttggtt gttaattgtc agttgtaagt tatttgttgt tagtggttcg
    12181 ttgtcagttg ttagtggttc gttgtcagtt gaaatttgct tgtaatttgg taattgtaaa
    12241 ttgttgtttc tcagttataa gttgttcttt gttagttatt agttggggac tcgggggatg
    12301 ggaaaaatag ggagtacatt tacattcgtt ttacagtgaa catgaaaaaa gccgctaact
    12361 tcatggagtg agcggctttt gaatgattag ttgttgagtt tatcttacga ctaatttacg
    12421 ggtaaatgta ttaccattca cgttgaaccg aatgaagtaa atacctgtct taagtgatga
    12481 aatatcgacc tgaagagttt gaattccctg tgcaaccctg ccataaatct ggcttgtgag
    12541 tttttgcccc gataccgtaa agatgtcgta gctaacagtt gcagatttat cgagggtaag
    12601 agagaaataa acctggtttt cggcaggagt aggataagcc tccgacaatg aaacatccag
    12661 catatttagc tgaggaatac ccacaccggg attattgctg gagaacacaa aatcagcgtc
    12721 agtgtaatag aaatcgggta tatatttaaa ctgaacccaa ctatccattg aggtaacatc
    12781 gcttaagcct tctattacaa tgggtatggt gtatttatta ttatctgtaa atacataacg
    12841 agaaaggcaa gcgaaaaaag attcgttata tacatcggag aaaagggtta cgttgttggg
    12901 taggtcgaca tacttgcctc cagtgagggt tccggtagtt ttgttggcca tcaagtcgaa
    12961 gccacggcag aaaacattag gagaattatt gttgtcggag aaggtggggt cggtatcgag
    13021 ccatgtaaca aaaaccttgg taccatccat agtactggct atctgagtac gattatcttc
    13081 cgagttgtca ccccaattac cgcggaattg ttgtggatag cccatttttt gagcatacca
    13141 atttatacct ccatcaattg aatagatatc gaaggcagcg aaagattttt cggcgactac
    13201 aatcgaataa tcgctattac ctgttacccc aacaattaca ccaatatgag ggttaccaaa
    13261 tttatcgacg actatatcgc agtcaaacgc tgtggtatag ccaatttctt cacgtgaagg
    13321 caaaggtgga ttatataaca ccgccatcat actatcgctt aatagtccgt acacaatttc
    13381 cttgatacca ttaggtccat cgagctgaac ttcgataggg ccctgccatg tttggccagc
    13441 atcggtcgat ttgaacagta taggataata attcagcatc cctggggtag tgagattttc
    13501 attattagct aaggcaacaa tccatgcagt ttgaccatca gggctgattg caactctttg
    13561 attggctgga cgattgttac tctctgtata aagttcaact gtctgaatct cgtaattaac
    13621 atctttggct acatcgtccc agagtccccg gaatacaatg atatttcctt gataaaccac
    13681 gcttcctgtt gccc
//
LOCUS       DAOORU010000050        13301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_5727_length_13301_cov_6.192511, whole genome shotgun sequence.
ACCESSION   DAOORU010000050 DAOORU010000000
VERSION     DAOORU010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13301
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5727_length_13301_cov_6.192511"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="PLF20_04700"
     CDS             complement(<1..1055)
                     /locus_tag="PLF20_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOX80339.1"
                     /translation="MKLRIYLILFCGLFLTVSLNAQPISPEYYLKTKGEVYFRFKADS
                     KQTVDELSKIISIDNVNAEGEVRAYANAKEFERFSNMRIAYEILPHPGDSPEAAIMST
                     FDEIKTRKNWNTYPTYDAYVQMMYDFATNYPNLCQVTQIGTTVQNRKLLVAKISSNVN
                     AIKPRFFYTSSIHGDETGPYVLCLRLIDYLLTNYGVDSRITHLLDNIEIYINPLANPD
                     GTYKGGNNTVNGAIRYNANYVDLNRNYPDPAAGPHPDGNAWQPETVAFMNFAEADQFT
                     QSANFHAGAEVANYPWDTWSRLTADNSWWVMVSNEYADTAQFYSPPGYFDDFGTGITN
                     GYQWYRITGGRQDYMNY"
     gene            complement(1064..1966)
                     /gene="dapA"
                     /locus_tag="PLF20_04705"
     CDS             complement(1064..1966)
                     /gene="dapA"
                     /locus_tag="PLF20_04705"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOX80340.1"
                     /translation="MKNKLKGTGVALITPFKQDYSIDYESLGKIIEYVLYNQVDYLVV
                     MGTTAETPTLSWEEKLELAAFARETVKGRVPLVYGIGGNNTSEVVASIRKADMHGYDA
                     ILSVTPYYNKPQQEGLYKHFMAIAEASPMPVILYNVPGRTGVNMTAETTLNLARSNSK
                     ICAIKEASGNLEQIAKIIKDKPQDFLVISGDDNLTLPILAIGGNGVISVVANVLPASF
                     STMVRWGLEEVFTEARKIHLALFEFTQYLFANGSPAGIKAALASLGMCEEVLRLPLVP
                     VTPELRSKIKQSLQNILNKQKNTI"
     gene            complement(1963..2397)
                     /locus_tag="PLF20_04710"
     CDS             complement(1963..2397)
                     /locus_tag="PLF20_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80341.1"
                     /translation="MILFEFKDKTDFEEVEEFSEFLRSLGKQVKLWGYYPHKNLPLFF
                     TPTGNQQCLTQKQLRWFPIPCERAIAGFNEYQPDILIDLSREDCYPLKYLAAISQASM
                     KVGCNSNFTQPIFDLLIQKDDDLPLTAFIENIIHYLNHLNKS"
     gene            2609..3379
                     /locus_tag="PLF20_04715"
     CDS             2609..3379
                     /locus_tag="PLF20_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX80342.1"
                     /translation="MRITFLGTGTSQGVPVIACECDICTSTDPRDRRLRSSVLIQEGA
                     MNIVIDAGPDFRCQMLRAGIKTLRAVLITHGHKDHIAGLDDVRPFNYFTHEPIDVYAA
                     REVQEDIRRDFFYAFDDDKYPGIPEIELHTIDYSPFSIDKLEVIPFELWHNRLKILGF
                     RIGDFAYITDASYIPESSMQLLTGLDVLVINALRLEPHVAHFNLPQALDIIHRLSPRS
                     AFLTHISHRLGKHGEIERILPPYVSVAYDGLEIEIDKS"
     gene            3435..4652
                     /locus_tag="PLF20_04720"
     CDS             3435..4652
                     /locus_tag="PLF20_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80343.1"
                     /translation="MENHRENSTVFHSVSVFFRKLKLLRRIRKKNKQAKKAARKKIRE
                     REKNIRKWERRKRRRLIRFVLKKQFHKLFPPKSKKKTQEPVKIKEEQKRKQASYSSWK
                     RRKRIIRFLIYKYLRSLSFGKKTGIQTQPASPAKFSRREKWIMMLNSASAFIIAYLTI
                     QIISQALTLLIAQNFEFQGTIRYWGVLYFITSNDWTPDSVLTLYSVQPLTSLILSLIG
                     LIIYLNVQQYEGILKMIFLWVYVYGLVAFFGALAVGTIINKGFGFVVIYIYLMDTAKL
                     LISLCSLGALLFIGVLSRSLFINSANVYFEEINFRNVKYVIRYHIVYPALLGSLVIIL
                     FKLPKITPYELFTLSTIIIFLLPVWTSPGIMGERIFEDSGNLTLRRKMLIFSFSILVL
                     FRLFTDPRLLASR"
     gene            5706..6623
                     /locus_tag="PLF20_04725"
     CDS             5706..6623
                     /locus_tag="PLF20_04725"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOX80344.1"
                     /translation="MSNFKGGNGGTERMKDFTQYIVYLFFRLFLALVRWFPMRLFYVL
                     SDLTFFILYKAIKYRRAITYDNLRKCFPEKDDGWIESTACKFYHNLSDILVESLKGFS
                     LSQKELSSRFKLANPEVFYEIEQKYPEIIVASAHCGNWEWGALLIPLLLKYHTYGIYK
                     PLSNRFIDDYLKKHRALWGMHLVSVTQTGFMFRNSAERRAYFFLSDQYPGKGRKVVEV
                     VFLGRPTLWLGGVEKYARRFRIPVYYFGIRRLERGRYEVFAEQISSNPHQSEFGEITR
                     NYVRILEQEILNHPDNWLWSHRRWKNLAE"
     gene            complement(6636..7190)
                     /locus_tag="PLF20_04730"
     CDS             complement(6636..7190)
                     /locus_tag="PLF20_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOX80345.1"
                     /translation="MKKFYHFLLKMLGWTIEGGCPEGNKFIIIQAPHTSLWDFVYGWL
                     FSRAIGIKPKFLIKSKYFFWPLGPILKMLGGVAVYPSKDTRFVKHLVQQIHSSKNFIL
                     VITPEGTRKKVKRWKTGFYQISLETGLPILLGRIDYSNKFIHLGNVFYPTGNMEADLR
                     KIQGYFRASWAKHPERYYEWPPTD"
     gene            complement(7230..7682)
                     /locus_tag="PLF20_04735"
     CDS             complement(7230..7682)
                     /locus_tag="PLF20_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80346.1"
                     /translation="MEQNSLKKKIYEVAVETLESAAERTRLAIEEAIQAAQEEVNTLD
                     IYESYRSQYLQKSEMFVSQYNKILEQLDILRKINPEEESQNIEFGSIVITDRQKIFVA
                     VGLGKIKVNGDTWFVISPQVPIFEAMKGKKAGDEFIFNNIKHKILQVS"
     gene            8016..8561
                     /locus_tag="PLF20_04740"
     CDS             8016..8561
                     /locus_tag="PLF20_04740"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HOX80347.1"
                     /translation="MDNKFSHPVYHRNVVEMLALAFEYCRFMEKLESIDKEGLASYLL
                     KVLPLLYVKGELLPEIEAEHPEANERFVIEEEWEGLFNYLRLILDSDDIYYTFDPPAY
                     GWGESLRTSLAEHLADIYQDMKDFVLLYQKNSLAAKENAVAECRRLFLEHWGPRVASC
                     LLAFHFMYHGGEKSYFTDPEQ"
     gene            8625..10586
                     /locus_tag="PLF20_04745"
     CDS             8625..10586
                     /locus_tag="PLF20_04745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX80348.1"
                     /translation="MKLSIIIVNYNVEYFLEQCLHSVRRACKNLEAEIWVVDNNSVDG
                     SLMMLNEKFPEVKIIANKENLGFAKANNQAIREAEGEYILLLNPDTLVEEDTFEKTIA
                     FMGSHPDAGGLGVKMVDGKGKFLKESKRGLPTPSVAFFKMTGLARIFPRSPLFARYYL
                     GHLSDDEIHEVEILSGAFMLLRREVLQKTGLLDEDYFMYGEDVDLSYRILKAGYKNYY
                     FPLTRIIHYKGESTRKSSVNYVFTFYQAMAIFARKHFSAGLARRFNFFINLAIFLRAS
                     LSLLRRFAQYVFLPLLDAALVYGGILVMKNYWEGFIFQGYGQYPPLFVYIILPAYIVV
                     WLLSVYLSGGYDKPFRLGKVIQGIALGTVIILAAYALLPENLRFSRALILFGALWAII
                     SMILTRVILHLSGIDQYHLAVNENKRFLIVGKNNECDRVAEILRNGIHRPAFIGYVAI
                     DPLQQQANGFIGNVQQMNEIIRIYGIDEIVFCASDLTAQFIIDQMLKLQDKSIEFKIA
                     PPESYSIIGSNSISTSNDPFIVDISSITSRRNKRNKRMADLFLVFLLMLTLPVNIWWV
                     ENKFGYLKNLFLVLIGRKTWVGLMDMPGWGEPSSAVKPGVLCPDDAYPSEHLTSQTAF
                     NLNLLYARNYRPLDDFSIVFKGFRNLGRI"
     gene            10752..11588
                     /locus_tag="PLF20_04750"
     CDS             10752..11588
                     /locus_tag="PLF20_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOX80349.1"
                     /translation="MNLKLTKPLAFIDLETTGLNVGHDRIVEISILKVYPDGTTKSYT
                     RRINPTIPIPAEVTRIHGITDDDVKHEPEFSSLGAEIAQFIKDCDLAGYNSNKFDIPL
                     LMEEFLRAGIEFDLKGRKLIDVQNIFHKMEPRNLEAAYRFYCNKDLVNAHSAEADIQA
                     TYEILLAQIERYAGKEVEMTQDSSPLLFDNTTSTLHQLSFHHKNVDFAGHIIYNEQGK
                     EVFNFGKYKGKSVEEIFRKEPSYYDWMMKAEFPLYTKKIIAAIKLRGFNKGRNNSNEG
                     KV"
     gene            11654..11842
                     /gene="rpmG"
                     /locus_tag="PLF20_04755"
     CDS             11654..11842
                     /gene="rpmG"
                     /locus_tag="PLF20_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOX80350.1"
                     /translation="MTKKNKEARVQVILECTEQKNSGVPGMSRYVTTKNKKNTPGRLE
                     LRKYNPYLRRVTVHKEIK"
     gene            11851..12006
                     /locus_tag="PLF20_04760"
     CDS             11851..12006
                     /locus_tag="PLF20_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HOX80351.1"
                     /translation="MAKKVVATLQTSGKDWAKVIRMVKSPKSGSYIFTEEMVPHDKVK
                     EFLASQK"
     gene            12106..13056
                     /gene="ftsY"
                     /locus_tag="PLF20_04765"
     CDS             12106..13056
                     /gene="ftsY"
                     /locus_tag="PLF20_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOX80352.1"
                     /translation="MALFSFFGKKEKEDLDKGLEKTKQSVFSKIARAIAGKSTVDDQV
                     LDELEDILIGADVGVDTTLRIIERIQKRVAREKYIHTSELNKILKEEIVALLEEHHSG
                     LIQGFDFEKGKYPYVIMVVGVNGVGKTTTIGKLAYQFARAGKKVILGAADTFRAAAID
                     QLKIWGQRANVTVIAQEMGADPAAVAFDTCNSALANDYDVVIIDTAGRLHNKLNLMNE
                     LSKIRKVIQKVIPEAPQEVLLVLDASTGQNAIEQARQFTSATEVNAIALTKLDGTAKG
                     GVVIGVSDQFKIPVKYIGVGEKIDQLQLFNRTEFVDSLFR"
     gene            complement(13173..13247)
                     /locus_tag="PLF20_04770"
     tRNA            complement(13173..13247)
                     /locus_tag="PLF20_04770"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13210..13212),aa:Val,seq:gac)
BASE COUNT         3842 a         2525 c         2790 g         4144 t
ORIGIN      
        1 aaatagttca tgtaatcctg tcgacccccg gtgatgcggt accattgata accattggtg
       61 atgcctgtac caaagtcgtc aaaatatccg ggaggactgt aaaactgagc cgtatcggca
      121 tattcgttgg aaaccataac ccaccaagaa ttgtcggctg taaggcggct ccaggtatcc
      181 caaggataat tggctacttc ggctccggca tgaaaattag ccgattgtgt aaattgatca
      241 gcttcggcaa aattcatgaa agcaacagtt tcgggttgcc acgcattccc gtcgggatga
      301 ggcccggcag caggatcagg ataattacgg tttaaatcta cataattggc gttatacctt
      361 atggcaccat ttacggtgtt gttacctcct ttataggttc catcgggatt tgctaaggga
      421 ttgatatata tctcgatatt atccaacaga tgagtaatgc gagagtcaac tccataattc
      481 gttagaagat agtcgattaa tctcaggcat aaaacatagg gccccgtctc gtctccatgt
      541 atagaagaag tataaaaaaa tcttggtttt atcgcattga cattgctgga aattttggct
      601 accaaaagtt ttcgattttg caccgttgtc ccaatttgag taacctggca gaggttggga
      661 taattggtgg caaaatcata catcatttga acataggcat cgtaggttgg gtaagtattc
      721 caatttttgc gcgttttaat ttcatcgaaa gtgctcatga tggcagcttc tggtgaatca
      781 cccggatgag gcaggatttc ataagctatt ctcatgttcg agaatcgttc gaattccttg
      841 gcattggcat aagccctcac ttcaccttcg gcattcacat tgtcaataga aataattttc
      901 gagagctcgt ccacagtttg tttcgaatct gctttaaaac gaaaatagac ttctcccttg
      961 gtttttagat aatattcggg agaaataggt tgggcattca gtgaaacggt taaaaacagt
     1021 ccacagaata gaataagata aattcttagt ttcataggcc gcattaaatc gtattttttt
     1081 gtttattgag gatattttgg aggctttgtt taatttttga tcgaagttca ggggttactg
     1141 gtaccagagg gagtctgagt acttcttcgc acataccgag tgaagcaagt gccgctttta
     1201 ttcctgcagg actaccatta gcgaaaagat attgtgtaaa ttcgaaaagt gctaaatgaa
     1261 tcttgcgggc ttcagtgaat acttcctcga gcccccatct taccatggta gaaaaagatg
     1321 cgggtaatac attggctact accgatatca ctccatttcc gcctatagct aaaattggga
     1381 gagtaaggtt atcgtcgcct gaaatgacta aaaaatcctg aggtttatcc ttgataattt
     1441 ttgcaatttg ttccagatta cctgaagctt ctttaattgc acaaatcttc gagttggatc
     1501 tggcaaggtt cagagtagtt tctgccgtca tatttactcc cgtacgtccc ggcacattgt
     1561 aaagaatgac gggcataggg ctggcttcgg ctatggccat aaaatgtttg taaagccctt
     1621 cctgctgagg tttattgtag taaggtgtaa cagaaagaat agcatcgtaa ccatgcatat
     1681 cggccttgcg aatagaggct acaacctcgg atgtattatt tccacctata ccataaacca
     1741 atgggactct gccttttacg gtttcgcggg caaaagcggc gagttccagc ttttcttccc
     1801 agctcaaagt cggagtctcg gcagtagtac ccattactac aagataatca acctgattgt
     1861 aaagaacata ttcgattatt tttccaagcg attcataatc aatcgaataa tcctgtttaa
     1921 agggagtaat caaagctact cccgtccctt ttaatttatt tttcatgatt tatttaaatg
     1981 atttaagtaa tgaattatat tttctataaa agcagttaga ggaagatcgt catctttttg
     2041 tatcagcaga tcgaaaatgg gctgtgtaaa gtttgaattg caacccactt tcatggatgc
     2101 ttggctgatg gcggcaaggt atttcaatgg gtaacaatct tcccgactta aatcgatgag
     2161 aatatccggt tgatattcgt tgaatcctgc tatggctcgt tcacacggaa taggaaacca
     2221 tctcaattgt ttttgtgtga ggcattgttg atttcccgta ggtgtgaaaa ataagggtaa
     2281 gtttttatgt ggataataac cccagagttt tacctgttta ccgagcgaac gcagaaattc
     2341 ggagaattct tcgacttcct caaaatccgt tttatccttg aattcgaata atatcatgag
     2401 cattcgtgat ccctccagat ttagaaattt tgcatctcgt ttcgtctggt gaattcttct
     2461 tgatatcgtc ttttttgcta gccgggtttt taagtttttc aatgaaagca ctatagctac
     2521 ttttttacac tttggtattg caaaattaga cataaattgc ccatttcccc tttactttcg
     2581 ttaattttaa cgtcgaattg ttcaagggat gagaattact tttttgggta cgggtacttc
     2641 acaaggcgtt ccggtaattg catgcgagtg tgatatttgt acttccaccg atccacgtga
     2701 tcgaagattg cgttcttccg tattgattca ggaaggcgct atgaatattg ttattgatgc
     2761 ggggcctgat tttcgttgtc agatgctaag ggcgggtata aaaacgctcc gagccgtttt
     2821 gataactcat ggacataagg accacatagc tggcctggat gacgtaaggc cattcaatta
     2881 tttcacgcat gaacccatag atgtttatgc tgctcgggag gttcaagaag acatccggcg
     2941 tgattttttc tatgcctttg atgacgacaa atatcccggc attcccgaaa ttgagcttca
     3001 tacgatagat tattctcctt tttcgataga taaactcgaa gtgatacctt tcgaattatg
     3061 gcacaaccga ctcaagattt taggattcag aataggagat tttgcttata ttactgatgc
     3121 cagttatatt cctgaaagca gcatgcaatt actgactggc ctcgatgttt tggtcatcaa
     3181 tgccctgagg ctcgagcctc atgttgcgca tttcaacctt ccccaagcgc tcgacatcat
     3241 tcatcgccta tcgcctagat ctgccttcct tactcatatc agccacagac tcggcaagca
     3301 tggcgaaata gaaagaattt tgcctcctta tgtttctgtt gcttacgacg gactcgagat
     3361 cgaaatagat aaatcatgat gtagaagtgt gaatcacccc ttttgtttat ttttgcggct
     3421 gatgggcctg gattatggaa aatcatagag agaacagcac ggtatttcat tcggtgtcag
     3481 tcttttttcg taaactgaaa ctgttgagaa ggatacgaaa gaagaacaag caggctaaga
     3541 aagccgccag aaagaaaatt cgtgaaaggg aaaaaaatat cagaaaatgg gaaaggagga
     3601 aaagacgccg tttgattcga tttgtcctta agaaacagtt tcataaactt ttccctccga
     3661 aatcaaagaa aaaaacacag gaaccagtca agataaaaga agaacagaag cgaaagcaag
     3721 ctagttattc ttcatggaaa aggcgaaagc gaatcatccg atttttgatt tataaatatt
     3781 taaggtcgtt gtctttcgga aagaaaacgg gcattcagac gcagccagct agccctgcca
     3841 aatttagccg cagggagaaa tggataatga tgctgaacag tgcctcggct tttatcattg
     3901 cttatctaac tattcaaatc atcagtcaag cacttacgtt gcttattgca caaaatttcg
     3961 aattccaggg aacgatacgc tattggggag tgctctattt tatcacatcg aatgactgga
     4021 cgcccgattc cgttctgaca ctttatagtg tgcaacctct tacaagcctg attttgtctt
     4081 tgattggctt gattatttat ctgaatgttc aacagtatga aggtattctt aagatgatat
     4141 tcttatgggt ttatgtttat ggtctggttg ctttctttgg agctctggcc gtgggtacca
     4201 tcatcaacaa gggttttggt tttgtggtta tttatattta tttgatggat acagccaaac
     4261 tgcttatctc gctttgttct ctgggtgctt tgctcttcat aggggttctg tcgagatctt
     4321 tgttcataaa ttctgccaat gtgtatttcg aagaaatcaa cttccgtaat gtcaagtatg
     4381 tcatcagata tcatatagtt taccctgcct tattgggtag ccttgttatc atcctgttca
     4441 aacttcccaa aattactcct tacgaacttt ttaccctgag caccatcatt atttttctac
     4501 ttcctgtatg gacatcgcca ggaattatgg gggagagaat tttcgaagac agtggaaatt
     4561 tgactctgag acgtaaaatg ttgatttttt ctttctcgat attggttctt ttccggctgt
     4621 ttaccgatcc tcggttatta gcgtcaagat agtgtggatt ggcctaccct gttttcgatt
     4681 cagattgaag gaaatttgtt ttcagcacat tttcgacaaa atcctttagt tcgcccggag
     4741 tgaatggttt agccagaaaa tggaagaaac cctctctcag caatctttct ggtttcgatt
     4801 tgaatgcgtt agcagccata acaacgatag ggcctcgaat tctatcggaa tatttatatc
     4861 ggaatgatga gaaaaacgtt cgaaacagat gttggcaaag ttgcggttgt ttttctgtat
     4921 caacactcag gttttttcga tgtgggtttt tataaaatag agtagggtga ttgagggagt
     4981 aaaattttcg gaaatgatcc tgaagaaaaa tttccctgcc tatggaaggc tgtttaatcc
     5041 gaaaaatttt tactcggata ataaaatgcc atagtagctc atgactgggc tcatttcgat
     5101 tggaatctat ttttattcac ttcgatatcc cattaacggt ttcgagattg tttacctcat
     5161 gatgccgaac aaaccctatg aaaattttca aacttaatcc gaaaatcctt ggcttattaa
     5221 aatcggataa atcgtcgaat ttatttacat gtatggggtc ggtatttcat gagtgaactt
     5281 ctgatcttag ttcaatattt agaaggggaa agggaaataa aagtctcttc catacgtttg
     5341 ttttgttgta gaatatggat ctgttggctt catcatgaat cgggaagcag aatatttatc
     5401 gcattcaggc atattgtacg attgtaaata aatagaataa aataatttag ttgagatttt
     5461 aaaggtttta tccggaattg tgaagagaat cgataaaata tttaggaata gctttatcag
     5521 ccatcacttt atagaaaatt ttcgatggaa atatatcatg cttaataata gtatggaaag
     5581 atgggggtta ttgtccattt tccggttagt tgccatttcg cggcttatgc atacgtcgaa
     5641 taaaaaaatt tgttcaacag acaggaaatt tgaaattatg gcaaccattt atttgagagc
     5701 tctttatgag taattttaag ggagggaacg ggggaaccga aagaatgaaa gattttactc
     5761 agtatattgt atatcttttt ttcagattgt ttttagctct ggtcaggtgg ttccccatgc
     5821 gtttatttta tgttctatcg gatttaactt tctttattct atataaagct ataaagtaca
     5881 ggagggcgat tacttacgac aatttgcgta aatgctttcc cgagaaagat gatggctgga
     5941 ttgaatcaac ggcctgtaaa ttttaccata atctttccga tattttagtc gagagtttga
     6001 aaggtttctc cctttcgcag aaagaattgt cgagtcgttt taaacttgcc aatccagagg
     6061 tattttatga aatcgagcaa aaatatcccg aaattattgt agcatccgct cattgtggga
     6121 actgggaatg gggagcattg ctcattcctc ttttgttgaa atatcatact tatgggattt
     6181 acaagccact ttccaatcgt tttattgatg attacctgaa gaagcacagg gctttatggg
     6241 ggatgcactt ggtttcagtg acacaaacag gtttcatgtt cagaaattct gccgagaggc
     6301 gggcgtattt ttttctttcc gatcaatatc cgggtaaggg tcggaaagtc gttgaggttg
     6361 tttttttagg taggcctacg ctctggttag gaggagttga aaagtatgcc cggcgttttc
     6421 gcattccggt ttattatttt ggaatcagaa ggttggagag aggcagatat gaagtttttg
     6481 ccgagcaaat ttcttcgaac cctcaccaaa gcgaatttgg agaaatcaca aggaattatg
     6541 ttcgtattct tgaacaagaa attttaaatc atcctgataa ttggttatgg tctcatagac
     6601 gatggaagaa cttggccgaa tgataaagat tagtcttaat cggtaggtgg ccattcgtaa
     6661 tatctttcag gatgcttggc ccatgaggct cgaaaatatc cctgaatctt tcgcaggtcg
     6721 gcttccatat tacccgtagg ataaaagacg ttcccaaggt gtatgaattt attggaataa
     6781 tcaatccttc caagaagaat gggcaagccc gtttcaaggc ttatttgata aaatcctgtt
     6841 ttccagcgtt tcaccttttt acgcgtacct tcgggagtaa ttaccaggat gaaattttta
     6901 gaagaatgaa tctgttgcac aaggtgtttg acaaaacgag tatccttact aggatagacc
     6961 gcaaccccac ccaacatttt caatataggt ccgagaggcc agaaaaaata tttcgatttg
     7021 atgagaaact tgggtttgat tcctattgcc ctcgaaaata accatccata aacaaaatcc
     7081 cacaaactgg tatgtggtgc ctgaatgatg ataaacttat ttccttcggg acaaccccct
     7141 tcaatcgtcc agccaagcat tttgagcaaa aaatggtaaa attttttcat gcggcttgtt
     7201 ttggaaggat agtcaatata gaaaataaac tatgaaactt gcaatatctt atgttttatg
     7261 ttgttgaaaa tgaattcgtc tcctgctttt ttccctttca tggcttcgaa gatgggcacc
     7321 tgaggtgaaa ttacgaacca agtatcgcca tttaccttaa tcttgcccag accaactgct
     7381 acaaatatct tctggcgatc ggtaatcaca atcgaaccaa attctatatt ttgtgattcc
     7441 tcttctggat taatcttcct taatatatcg agttgttcca atattttatt gtattgactg
     7501 acaaacattt cgctcttctg aagatattgc gatcggtagg attcatagat atccaaagta
     7561 tttacctctt cctgagcagc ttgtatggct tcttcaatcg caagcctcgt tctctcggca
     7621 gccgattcga gagtttctac agctacttca tatatcttct tctttaatga attctgttcc
     7681 ataatcggtt taacttgaat aggataaaaa aaaacctccc gacaaaggag acattttatg
     7741 ccgggaaagg tttctttgca aatatataaa gaaatgaaat atgcaaatga attttaataa
     7801 aaaatttata agtgtttgcg ctcatctttg ccattcattc ctccctgttt ttttaccaat
     7861 ttttttcttc taaaagctcg atttatgcgc cgattctttg ttcgttgttt aacgatcgta
     7921 atttttggag aaggttcggg aaaattttag tcaaggtttt tatagagatg gtatccatag
     7981 aaagcttacc tttgttgagg tgaatgtgta atattatgga taacaaattc agtcatcctg
     8041 tttaccatcg taatgtagtg gagatgctgg cattggcctt tgagtattgc cggttcatgg
     8101 aaaaattaga aagtatagat aaggaaggtc tggcctcata tttactcaaa gtattgccgc
     8161 tgctatacgt gaaaggagaa ttattgcccg aaatcgaagc tgagcatcct gaagccaatg
     8221 aacgtttcgt gatagaagaa gaatgggaag ggctttttaa ttatctcaga ctcatattag
     8281 attccgacga tatttattac accttcgatc ctccggctta tggctgggga gaaagtttaa
     8341 ggaccagtct ggcagagcat ctggccgata tttatcagga tatgaaagat ttcgtgttat
     8401 tgtatcagaa gaattcgctt gctgccaaag aaaatgcagt agccgaatgc cgccggcttt
     8461 tcctcgaaca ttggggccct cgtgtcgcat cttgtttgct tgctttccat tttatgtatc
     8521 atggaggcga aaaatcttat tttaccgacc cagagcaata agatatttta tgctatcgtt
     8581 aattaggcta acatattcat ttatgctttt ctacctttcc ttcgatgaaa ctttcaatca
     8641 ttatcgtcaa ttataacgtt gaatattttc ttgaacaatg cttgcattca gtaaggcgag
     8701 cctgtaaaaa cctcgaggca gaaatatggg tggtcgataa caatagcgtg gatggctcgt
     8761 tgatgatgct gaacgaaaaa tttcccgaag taaaaataat tgccaataaa gaaaacctgg
     8821 ggtttgccaa agcaaataat caagctatac gtgaggcaga aggagaatat attctcttgc
     8881 tgaatcccga taccctagtg gaagaggaca cattcgaaaa aaccatagcc tttatgggtt
     8941 cacatcctga tgcaggtggg ttaggagtga agatggtaga tggcaaggga aaattcctga
     9001 aagaatctaa acgaggctta cctactcctt cggtggcttt tttcaagatg acaggactag
     9061 caagaatatt tccccgcagt cctttgtttg cccgttatta tcttggccat ctttccgatg
     9121 atgagattca cgaagttgaa atcctttcgg gtgcttttat gcttttgaga cgtgaagtgc
     9181 ttcaaaaaac cggtttgctc gatgaagatt atttcatgta tggggaagac gttgatttgt
     9241 cgtacagaat tttgaaggcc ggatacaaga attattactt tccactcacg cgtatcattc
     9301 attacaaggg tgaaagtaca cgtaagagct cggtcaatta tgtctttacg ttttatcaag
     9361 ccatggccat ttttgccaga aagcattttt cggccggctt ggcacgcagg ttcaactttt
     9421 ttatcaatct ggctattttt ttgagggcta gtctttctct tttgcggcga tttgctcaat
     9481 acgttttttt acccttgctg gatgctgcct tggtttatgg tggtattctg gtgatgaaga
     9541 attactggga gggttttatc tttcagggat atggacaata ccccccactt ttcgtttaca
     9601 tcattttacc ggcttatatt gttgtctggc tactttcggt gtatttgagc ggaggctacg
     9661 acaaaccctt tcgtttagga aaggttattc agggaattgc gctgggtact gtgattattt
     9721 tggctgctta tgcgttattg cccgaaaatt tgagattttc gcgcgctctg attctttttg
     9781 gtgcattatg ggctattatc agcatgatac tgactcgcgt aattcttcat ttgagtggta
     9841 tcgatcaata tcacctggct gtaaacgaga ataaacgatt tctgatagtt gggaaaaata
     9901 acgaatgcga tcgggtggcc gaaatcctgc gtaatggaat ccacagaccg gcttttattg
     9961 gttatgtagc catcgatccc cttcagcaac aagccaacgg ttttatcgga aatgtacaac
    10021 aaatgaacga aattattcga atctatggta tcgatgaaat cgttttttgc gccagcgatc
    10081 ttacggctca attcatcatc gatcaaatgt tgaaacttca agataaatcc atagaattca
    10141 aaattgcacc tcccgaaagc tattctatca ttggcagcaa ttctataagc acttcgaacg
    10201 atccttttat cgtcgacatc agcagcataa ccagtcgtcg caacaagcgt aataagcgga
    10261 tggcagatct cttcctcgtt ttcttgctaa tgctcactct tcctgttaat atttggtggg
    10321 tcgaaaataa attcggatat ctgaaaaatc ttttccttgt gctaataggt cgcaaaacct
    10381 gggtaggcct catggatatg ccgggctggg gagagccatc ttctgcagtt aaacctggag
    10441 ttttatgccc tgacgatgcc tatccttccg aacacttaac atctcaaacc gcgttcaacc
    10501 ttaatttgct ctatgcccga aattacagac ctttggatga tttcagtatc gtattcaaag
    10561 gctttcgcaa tcttggacga atttaaatcg gctgatttcg tatgaatctc gtcttactta
    10621 ttgatgacag aaaactcaaa aacccaaatt tttcgtttat ggaatcgtcg atggtttatc
    10681 atggaccaat tcaggtctat acgttattta ttctaatttt gccttagaaa tgaattattt
    10741 gctaaaggaa aatgaattta aaactaacca agccacttgc atttatcgat cttgaaacca
    10801 ctggtttaaa cgttggccac gatagaattg tcgaaatcag tattctgaag gtatatcccg
    10861 atgggacaac caaatcctat actcgccgca taaatcccac cattcctatc ccggccgagg
    10921 tgacacgcat acatgggatt accgacgatg acgtaaaaca tgaacctgaa ttttcgagtt
    10981 taggtgcaga gattgcccaa tttataaagg attgtgatct ggcaggttac aatagcaata
    11041 aattcgacat acctctactc atggaagaat ttcttagagc tgggatagag ttcgatttaa
    11101 aaggcaggaa gttgatcgat gtgcagaaca tttttcataa gatggaacct cgaaacctgg
    11161 aggctgcata tagattctat tgcaataagg accttgtaaa tgcccactcg gccgaagccg
    11221 acattcaggc tacctatgaa atccttctgg ctcagattga acgatatgcc ggcaaagaag
    11281 tcgagatgac ccaagattcc agcccgctct tattcgacaa caccacttca accttgcatc
    11341 agctttcttt tcatcacaaa aatgtcgatt ttgcgggtca tattatttat aatgagcaag
    11401 gaaaagaagt gttcaatttt ggaaaatata aaggaaagtc ggttgaagaa attttccgca
    11461 aggaaccttc ctattatgac tggatgatga aagccgaatt tccgctttat accaagaaaa
    11521 tcattgcggc tatcaagctt cgagggttca ataagggccg gaataattcg aacgaaggaa
    11581 aagtttgaga tttttatttt gttatcgaaa aatatgtaat tttgcacact taattcaaaa
    11641 tggataccga gagatgacaa agaaaaataa agaagcaaga gtacaggtaa ttctggaatg
    11701 caccgaacag aagaactcgg gcgtgccagg catgtcgagg tatgttacta ccaagaacaa
    11761 gaaaaatacc cctggccgcc tcgaactgag gaaatacaat ccatatttga gaagagttac
    11821 cgttcataaa gaaattaaat aaattgtact atggcaaaga aagtcgttgc aaccttacaa
    11881 acttcaggaa aagactgggc aaaggttatc cgtatggtga aatcgcccaa gtcaggctct
    11941 tatattttta ccgaagagat ggtgccccac gataaagtaa aggaatttct ggcttctcag
    12001 aaatagtttt ttcataagca cttctgcaaa aaaacaggga gccttcgaaa aacaaaaggg
    12061 ggctccctgt tattttattt taataagtat attttgttga ctactatggc attattttca
    12121 ttttttggta agaaggaaaa agaagatctc gacaaaggat tggaaaaaac aaagcaatcg
    12181 gttttttcta aaattgcaag agccattgct ggaaaatcga cggttgacga tcaggtgctc
    12241 gatgagctcg aagacattct cattggcgcc gatgttgggg ttgacaccac gcttcgtatc
    12301 atcgagagaa ttcaaaaacg tgtagctcgc gaaaaatata tccatacctc cgaactcaac
    12361 aaaattctga aagaagaaat tgttgccttg ctcgaggaac atcattcggg cctgatacaa
    12421 ggtttcgact tcgaaaaagg gaaatatcct tatgtgatta tggtggttgg ggtaaatggc
    12481 gtgggtaaga ctacaaccat tggaaagtta gcctatcaat ttgctcgggc agggaaaaag
    12541 gtaatcctcg gtgctgccga tactttcaga gccgcagcca ttgatcagct taaaatctgg
    12601 ggacagaggg caaacgtcac cgtaatagct caggaaatgg gtgccgaccc ggcagctgtg
    12661 gctttcgata cctgcaactc ggccttggct aacgattatg acgtggtgat cattgatact
    12721 gccggccgtc ttcacaacaa gttgaatctt atgaatgaac tttcaaaaat cagaaaagtc
    12781 attcaaaaag tcatccccga agctcctcag gaagtactgc ttgtattgga tgcttcgacc
    12841 ggccaaaatg ccatcgaaca ggctcgacaa ttcacttcgg ctaccgaagt aaatgccatt
    12901 gccctcacca aactcgatgg aacagctaag ggaggtgtgg ttatcggcgt ctccgaccag
    12961 tttaaaattc cagtgaaata tataggtgtg ggtgaaaaaa tcgatcagct tcaactcttt
    13021 aaccgcaccg aattcgttga cagccttttt agataatcta gaagattttt ttacggaaag
    13081 atggacaaat ctaaacctgt tcttttgtcc tattccagtt ttttatttgt ttcgacatag
    13141 atcctaaaga ggattcgaaa gaatttatct tgtgggatgt acaggactcg aacctgtgac
    13201 ttccaccctg tcaaggtgac actctaaacc aactgagtta acatcccgat tatggcttac
    13261 aaaattagca agtttttccc tactcacaag attcaggcag t
//
LOCUS       DAOORU010000051        13038 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_5896_length_13038_cov_4.746438, whole genome shotgun sequence.
ACCESSION   DAOORU010000051 DAOORU010000000
VERSION     DAOORU010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13038)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13038)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13038
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5896_length_13038_cov_4.746438"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            40..168
                     /locus_tag="PLF20_04775"
     CDS             40..168
                     /locus_tag="PLF20_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80353.1"
                     /translation="MSKSGVMIRVFYDSSITNPYVSNQYSTRTSMGGISLRFTLAQ"
     gene            complement(183..743)
                     /locus_tag="PLF20_04780"
     CDS             complement(183..743)
                     /locus_tag="PLF20_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX80354.1"
                     /translation="MMHIIGITGTLGAGKGTIVEYLKDKKGFAHFSVREFLKEILLMQ
                     NKPVNRDNYTLCANQLREKFGPSYIIDCLYEKALAKGCNAVIESIRTPGEFYSLRQKG
                     NFTLFAIDADPEIRYERIKQRNSETDQITFEEFLASERREMQSDEPHHQNITLCISLA
                     DYKFLNNGTKEELFELVEETLKQMGL"
     gene            complement(743..1099)
                     /locus_tag="PLF20_04785"
     CDS             complement(743..1099)
                     /locus_tag="PLF20_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOX80355.1"
                     /translation="MAERKELMGKLSDAFIALPGGIGTLDELFESLSWLQLDIYNKPI
                     GILNVNHYYDPLLTFLDHATDAGFIIEQHRENLIVANDASDMLIKIRQFVPHSPPATW
                     IKQLIDDTQVKLTDHL"
     gene            complement(1123..1437)
                     /locus_tag="PLF20_04790"
     CDS             complement(1123..1437)
                     /locus_tag="PLF20_04790"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80356.1"
                     /translation="MIFTNQQVLVQINRVFLTLLIQLNKMEIHNIGVFCGSSMGDHAI
                     YRQMAENLGKEIAQAGITLVYGGSNLGTMKALADACLKESGKVIGIMPRFLQGKKFFT
                     KD"
     gene            1523..2512
                     /locus_tag="PLF20_04795"
     CDS             1523..2512
                     /locus_tag="PLF20_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HOX80357.1"
                     /translation="MPTLYPLKFRPIYKDKIWGGQRLKTLLNHDFSPLPNCGEAWLIS
                     GIEENNSIVSNGFLADNELNELVEVYMDELMGESVFERYGNEFPLLLKLLDANDWLSI
                     QVHPNDEIAKARGLSGGKTEMWYIIHAEAGSELIAGFKKKISRFELQQLIHQKRISEV
                     LNYETVKAGDVFYMPAGRIHSLGPGIVLAEIQQSSDTTYRIYDWDRVDDNGQSRQLHL
                     DEAIDAIDFNLYTDYKTKYTIIPNRTTHLVQSPYFTTNLLYLNHPIEKDYGYIDSFVI
                     LLAVEGEVGISDETGNYSLRTGEAMLLPAITEKVIFTTATPSASILETFIDLD"
     gene            2725..3807
                     /gene="fbaA"
                     /locus_tag="PLF20_04800"
     CDS             2725..3807
                     /gene="fbaA"
                     /locus_tag="PLF20_04800"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOX80358.1"
                     /translation="MMTEKISDKLKPGVVTGEGVSEIFRIAKNNQFALPAVNVVSSHT
                     IIAALEAAREVHSPIIIQFSNGGAAFLAGKGLSNEGERAAILGATIGAKYIWHLAEAY
                     GVPVIIHTDHAAKKLLPWIDGLLEVGEEHFRKYGKPLFSSHMLDLSEEPLEENIDICK
                     KYLERMSRLDMTLEIELGVTGGEEDGIDHTGIENARLYTQPEHVAYAYEQLLKISPNF
                     TIAASFGNVHGVYKPGNVILRPIILHNSQQYIEKKLNTPPKPVNFVFHGGSGSSQDDI
                     REAISYGVVKMNIDTDTQWAQWCGILNYYKKYSGYLQSQIGNPEGEDKPNKKYYDPRK
                     WMREAEISMVNRLKQAFDDLNALNRF"
     gene            complement(4325..5170)
                     /locus_tag="PLF20_04805"
     CDS             complement(4325..5170)
                     /locus_tag="PLF20_04805"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOX80359.1"
                     /translation="MKEIIAAVDFSECSVNAAEHALSIAAHAMTDLKLVYVFNKNQNQ
                     KTIFKYHDPIDEATQLLEELKGKFQPMLPENSVLYKIREGKVYKEVVTEANESNALMI
                     VTGTHGSSGFEELWIGSNAYRIICSANCPVITLREGVKPERDLRRIVLPIDETLTTRQ
                     KVPHATFLASLFDAEIHVLALFQSSMKEIQDKVIKYAKQACAHLEKNKIKFLMTSVKC
                     KNPTDATIEYATKVDANLIVIMTDQTTSFKNLFLGPYAEQMIHRSPIPVMSIRPKDLI
                     SVMSR"
     gene            complement(5248..5661)
                     /locus_tag="PLF20_04810"
     CDS             complement(5248..5661)
                     /locus_tag="PLF20_04810"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOX80360.1"
                     /translation="MSKKLSLLILLLAAFVLHSFGQFNGKVEIIQDPLIDTLIQRHIQ
                     INQLQPTIEGYRIQIYFESGNQARTLANRIKERFEELYPDYGAYLTFNEPYYRVRVGD
                     FRDKMSAEAFRQILLQDFPNAFIVPDNVYFEKIQN"
     gene            complement(5675..6505)
                     /locus_tag="PLF20_04815"
     CDS             complement(5675..6505)
                     /locus_tag="PLF20_04815"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOX80361.1"
                     /translation="MKNIIVPIDFSAGALYALKYAVGVASKAKSNLTLVWVDNQVNQL
                     PFMASSSNDMREEAKARMIEIIEKYQKKISGKLEYKLRKGKVYTEVCNLARQKEADLI
                     IAGTYGGSGFEKFWIGSNAYRLVTHAPCPVITVRFVSPVKFSLKKIIMPVDNSPDTLQ
                     KLGITVEFARIFQAEVHVLSLLTEKLRTLRMRTEANTHEALKYLEKYNVKSQLTVYET
                     GNITATLLDYATNIKADLISIMTEQELGESQALLGPNARIVIKESSVPVLSSHSNTKI
                     "
     gene            6735..7757
                     /gene="ruvB"
                     /locus_tag="PLF20_04820"
     CDS             6735..7757
                     /gene="ruvB"
                     /locus_tag="PLF20_04820"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOX80362.1"
                     /translation="MNQNLDASGKSLTPAEREIEKVLRPLEFSDFAGQTGIVDNLKIF
                     VAAAYQRGEALDHVLLHGPPGLGKTTLSYIIANELGVGIKVTSGPVLEKPGDLAGLLT
                     SLRHRDVLFIDEIHRLSPMVEEYLYSAMEDFKIDIMIETGPNARSVQIRLNPFTLIGA
                     TTRSGLLTAPLRSRFGINARLTYYTADVLEKIILRSSGILKVKIDEEAAIEIARRSRG
                     TPRIANLLLRRVRDFAQVIGDGVINMKIATYALNALNVDSHGLDEMDNKILRTIIEKF
                     GGGPVGLNTIATSVSEDPGTIEEVYEPFLIQEGFLQRTPRGREATELAYKHIGKLPPS
                     VQGRFF"
     gene            7771..8721
                     /gene="queG"
                     /locus_tag="PLF20_04825"
     CDS             7771..8721
                     /gene="queG"
                     /locus_tag="PLF20_04825"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOX80363.1"
                     /translation="MDTLSIKSKIRQQAFDIGFSKCGFAVSRFLTSDFEHYKKQIALG
                     FHADMLYLEKEAEKRFHPLLVHPDIRTVIVLLAGYKTNARQKDGAFYSISDYALIPDY
                     HKIMKEGLQKLSCFIENEFHSDVLINFVDTGHISEKAWAREAGLGNFGKNSILLTPEG
                     SYFFIGVILTDLVIIPDEAFDEDLCKNCDLCIKSCPTQAIHQPYAVDARLCISYHTIE
                     NKGHIPSNIIEKIGTQIYGCDICQKVCPWNRHQTVNSVLFSPLPPLLDMKAEDFESVT
                     ETDFRERFHESPMVRLGHRRYMRNIEAVKNMAATERKRPY"
     gene            complement(8736..12578)
                     /locus_tag="PLF20_04830"
     CDS             complement(8736..12578)
                     /locus_tag="PLF20_04830"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOX80364.1"
                     /translation="MKRLLLLFGLLFCMLASRAGEWIPLTSEEPQKAGIHLISSDITS
                     SVIEFQIKGFYLKPVQTPRGIENIVEVGNSSRILAAGAPDLPKLTASVIIPDEAMMGI
                     RILSSSYTDYTGIEVAPSKGNFTRDIDPASVPYTYGPEYQSNHFFPQTEGELREPYIL
                     RDFRGQTLIVYPFFYNPVTKVLRVFSELRVEIYKIGDHGLNPYLRKTPQIKIQQEFSQ
                     VYQKQFLNYNPTDYTPLPDFGKMLVICYPDFMASMQPYVDWKNSIGIPTEMVNVATIG
                     STSAVIKNYIVNYYNTNTLTFVLLVGDGPQIPTNTGSGLGGPSDNAYGYIVGNDHYID
                     VFIGRFSAENVAHVQTQVSRTLEYEKNPSFLNDDWYTTCLGIASDQGPGDDGEYDYQH
                     VRNMQTDLLGYTYTWNPELFDGSQGGNDAPGNPTPAMVSSEVNNGTSIILYTGHGSTT
                     SWGTSGFSNANVNQLTNVGKLPFIWAVACVNGEFMNTTCFAEAWLRATQNNQPTGAIS
                     FLGSTINQSWNSPMEGQDEMVDILVETYPNNIRRTFAGLSLNGCAKMIDSYGTDGSNM
                     ADTWTVFGDPSLMVRTANPQPLSATFNPTIFVGASTFTINCNVNGARVTLSNNGTILN
                     TTWVANGTATLNFTPLITPNDTLTLTVVEYNYIPLIAQIPVIPAVGPYVIYESTQIAD
                     FTTGNGNGLLDYSESSNLTISVKNIGVDSSVNTWVSLRSLSPYVEITDSIENYGTIPA
                     GEIISVDEGFQLSVSPFIPDHQVIPFTLIVQDSSNSWTSQFSLEAHAPVLQEGEISVN
                     DSLGNNNQRFDAGESVYVNIPVINIGSSAAYNLIGTLTTADPYLLISTKEATYGDILP
                     LGSQSQAFLISALPTVPPGYQAMITLTLMGDFGWTASYDFTLTIGLIPVLVIDLDGNN
                     NSGPTMQAALNQLNVANEYTTSFSTDLSKYKSLFVCLGVYPNKHILSAAEGLVLKNYL
                     NQGGRIYMEGGDTWYYDQQSTPTPLHPMFQIKGLKDNGGTLTTIAGKTGTFTEGMSFV
                     YNGDNNYIDKIEPINQAFTVFMNTSPSYIVTIANDPGSVYKTIGSSNEFGGLTDNVSP
                     STKKDLMLQYLTFFDIFGSPYWGNFVGIPTTIGINDTVEFFDLSSTTFTSRIWSFPGG
                     EPSTSTELNPKIVYPSMGTYDVTLEVSSSDSTIIIIRNDYISVYDYTGINPQSVSTEI
                     ILYPNPLSRNFGALNIRSSNANIQSIRIFNSTGMEVFSQMNYMESSPISLNHLKPGLY
                     LVEIKTDQGIATQKLMIR"
     gene            complement(12718..>13038)
                     /locus_tag="PLF20_04835"
     CDS             complement(12718..>13038)
                     /locus_tag="PLF20_04835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80365.1"
                     /translation="VSSPSNSLYVEVVGITKIPQDAFFLVFPNPFSNLLNISYTLQNS
                     TEVKIEILNSLGQTIANPLIEQKSEAGSYQIQLNTTSFKPGIYYIRFTTGTSSIVVKA
                     LLMK"
BASE COUNT         3817 a         2401 c         2837 g         3983 t
ORIGIN      
        1 atctcattgt gtgattgttg taaaaatgtc ttttaaaatg tgtcgaagtc aggagtaatg
       61 atacgggtgt tttacgatag ttccatcacc aatccctatg tctccaacca gtattctacc
      121 cgaacttcga tgggtggtat cagtttgaga tttactctgg cccaataaat ttcaacaaga
      181 tttcaaagcc ccatttgctt caaggtttct tccaccagct cgaaaagctc ttcttttgtt
      241 ccgttgttca aaaatttata atcggcaagg ctgatgcaga gtgtaatatt ttgatgatga
      301 ggttcatccg attgcatttc tctgcgttcg ctggctaaga attcttcgaa ggtgatttga
      361 tcggtttcag aattcctctg ttttattcgt tcgtatcgga tttcggggtc ggcatcgatg
      421 gcaaacaagg tgaaatttcc tttctggcgt agtgaataaa attcgccagg ggttctaata
      481 ctttcgatga ctgcattgca gcctttcgct agtgcctttt catacaggca atcaatgatg
      541 taggaagggc caaatttttc tcttagctgg tttgcacaca gggtgtaatt atctcgattt
      601 acaggtttat tttgcatgag cagaatttct tttaaaaatt ctctgaccga gaaatgggca
      661 aatccctttt tatctttcaa atattcaacg atggtgcctt tgccggctcc taaagttcct
      721 gtaattccta taatatgcat cattataaat ggtctgtgag ttttacttgc gtatcgtcta
      781 tcaattgttt tatccatgtt gccggaggcg aatggggaac aaactgtcga atctttatga
      841 gcatgtcgga agcatcatta gctactatga gattttcccg atgttgctca atgataaaac
      901 ctgcatcggt agcatgatcg agaaaagtga gaagagggtc gtaataatgg tttacattga
      961 ggatgccaat gggtttattg taaatatcga gttgaagcca tgaaagtgat tcgaacaatt
     1021 catcgagcgt accgatacct ccaggcaggg caataaaagc atcggataac tttcccatta
     1081 gttccttacg ttcggccata gattcgcaag tataaacctg agtcaatcct tggtgaagaa
     1141 tttctttcct tgcaggaaac gaggcataat tccaatgact ttccccgatt ccttcaagca
     1201 agcatcggct aaagccttca tggttcctaa gttggaacct ccatacacca aggttatacc
     1261 tgcttgggct atttcttttc ccagattttc tgccatttgt ctgtaaatgg catgatcccc
     1321 catacttgaa ccgcaaaaaa caccaatgtt atgaatctcc atcttattaa gctgaatgag
     1381 caaagttagg aaaaccctgt taatttgcac taaaacctgc tggttcgtaa aaatcatgta
     1441 tgagttctta actttatgaa gaattgaaat gccatgctgc ttcgattgaa aaatagtatt
     1501 tttgttgtga taattcataa acatgccgac actctatcct ttgaagttca ggcccatata
     1561 taaggataaa atatggggcg gacaacgatt aaaaaccctt ctgaatcatg atttcagtcc
     1621 tttaccaaat tgtggcgaag cttggctgat ttcgggcatc gaagagaata attctatagt
     1681 ttccaatggt tttttggccg ataatgaatt gaatgaactg gtggaggttt atatggatga
     1741 gcttatgggt gaatcggtgt tcgaaagata tggaaatgaa tttcctttgc ttttgaagct
     1801 tctggatgcc aatgattggc tgagtattca ggttcatcca aatgacgaaa ttgccaaggc
     1861 aaggggattg tctggtggta aaaccgaaat gtggtatatt atccatgccg aagcaggttc
     1921 tgaattgatt gctggcttta agaagaaaat ctcgcgattt gagcttcagc agttgattca
     1981 tcagaaacgc atttctgaag tcttgaatta tgaaacggta aaggcaggag atgttttcta
     2041 catgcctgca gggcgcatcc attctcttgg cccaggaatc gtattggccg aaattcagca
     2101 atcgagcgat acaacctatc gaatttatga ttgggaccgt gtagatgaca atggccaaag
     2161 ccggcaactt cacctcgatg aagccatcga tgcgatcgat tttaacctgt ataccgatta
     2221 taaaacaaaa tatactatta ttcccaacag aaccacacat ctggttcaat ctccctattt
     2281 tactaccaat ctactgtatc tgaatcatcc catcgagaag gattatggat atatcgattc
     2341 ctttgtgatt ttactggcgg ttgaaggaga agtcggaata tctgacgaaa ctggtaatta
     2401 ttctttgcgt acaggcgaag ccatgctttt acctgctatt accgaaaaag taatctttac
     2461 tacagcaacg ccttcagcct ctatattgga aacttttatc gatttggatt aaatcatact
     2521 tatttccatt ttatgttaat tcatttttat agaacaacat agactttatt tgcaatcgaa
     2581 tggtttgcta cgtttcggtt ttgtcctcat gttatgcaat aataaacagc agagagtttt
     2641 taaattacgg ggaaatgttg tagttttgta gtatcgatga aatgaagttt agctgattta
     2701 tcaacccata aaaaccattg aattatgatg acggaaaaaa tttcggataa attaaagcct
     2761 ggagtagtaa caggtgaagg agtaagtgaa atatttcgta ttgctaagaa caaccagttt
     2821 gccttaccag cggtcaatgt cgtaagttcg cacactatta ttgctgcctt ggaggctgcc
     2881 agagaggttc attctcccat catcattcag ttttcgaatg gaggtgcagc atttttagca
     2941 ggtaaaggtt tgtcgaatga aggtgagcgg gcagccatac tcggggctac tataggtgcc
     3001 aaatatatct ggcatcttgc cgaggcctat ggtgttcctg taatcataca taccgaccat
     3061 gcggcaaaga aattgcttcc ctggattgat ggccttctcg aggttgggga agaacatttc
     3121 aggaagtatg gaaagccttt attcagctcc cacatgctcg acctttccga agaaccactc
     3181 gaagaaaaca ttgatatttg caagaaatat ctcgaaagaa tgagccgctt ggacatgact
     3241 ctggaaatcg aactcggggt taccggcggt gaagaagatg gaatcgacca tacaggcatt
     3301 gagaatgccc gcctttatac ccaacccgaa cacgtcgcct atgcttatga gcagcttcta
     3361 aaaatcagtc cgaattttac catagccgct tcattcggta atgtgcatgg cgtttataag
     3421 ccgggtaatg ttatattgag gcctatcatc cttcataatt ctcaacaata catcgagaaa
     3481 aaactcaata ctccgcccaa acccgtaaat tttgtgttcc atggtggctc tggttcgagt
     3541 caggacgaca ttcgtgaagc cattagctat ggtgtagtca aaatgaacat cgataccgat
     3601 actcaatggg cgcaatggtg tggtatcttg aattattata agaaatatag cgggtatttg
     3661 caaagtcaga ttggaaatcc cgaaggtgag gataaaccca ataagaagta ctacgatcca
     3721 cgcaaatgga tgcgcgaggc cgaaatatct atggtaaacc ggttgaaaca agctttcgac
     3781 gaccttaatg cattgaatag attttgaaaa ttgttttgaa agctaagctt ctaaatataa
     3841 aatagcaaaa ttatttgctt cctgctcttc ttccatatat cgtacaagag gaaagaagac
     3901 attttacgag ttataaaagg gcagagacaa tggcaaagtg tgatggaaag gttgttttaa
     3961 tggtcgtgga tttgccttat aattatgaac ttctcataaa tattattaga tttcggcgag
     4021 gatttagata gtatgggttg agaatggccc taaggccact ctttatgtag aagatgaaaa
     4081 cagctccttt agatattttt cgaggaatat tgatttacct attgaatgtt tatgttggca
     4141 tgaaaacgat aaagcacttg aacccgcaat tgtctattat cgctcgaatg gcttatgttt
     4201 atgaaggtga acaggaatga agtcgtgaaa aaggcttgca tgattatatc ctcgcaatta
     4261 tgcgaaggga aatccttttc gaaaagatgg aaaattttca gaggtgatcg caattagaag
     4321 aatcttatct cgacataaca ctaatcaagt cttttgggcg aatactcata acaggaatag
     4381 gagaacggtg aatcatttgt tcggcgtagg ggccgagaaa taaattcttg aacgaagtgg
     4441 tttgatcggt cataattacg atgaggtttg catccacttt agtagcatat tcaattgtag
     4501 catcggttgg gtttttacat tttaccgaag tcatcaggaa tttgattttg tttttttcga
     4561 gatgtgcaca ggcttgtttt gcgtacttga ttaccttatc ctgaatttct ttcatgctac
     4621 tctggaatag ggccaggaca tggatctcgg catcaaacaa gctggccaaa aaggtggcat
     4681 gtggtacttt ttgtcgagtt gtaagggttt catcaatggg aaggacgata cggcgcaaat
     4741 cgcgttctgg ttttactcct tcgcgtagag ttattaccgg acaattggcc gaacaaataa
     4801 tccggtaagc attgcttcca atccataatt cctcgaatcc tgaactgcca tgcgtgcctg
     4861 ttacaatcat caaagcattg ctttcattgg cttcggttac cacttctttg taaaccttgc
     4921 cctccctgat cttgtataaa accgaatttt cgggcagcat aggttgaaat ttgcctttta
     4981 actcctcgag taattgagtg gcttcatcaa taggatcatg gtatttgaaa atggtttttt
     5041 gattttggtt tttattaaaa acatacacca gttttaaatc ggtcatggca tgtgcagcaa
     5101 tacttaaagc atgttcggct gcattgacag aacattccga aaagtcgaca gctgcaataa
     5161 tttctttcat gggtttgggt tttaagttgc tttaattatt atgtgtaata gttataattt
     5221 aatattttac aaaaattcta caatttctta attttgaatt ttctcgaagt aaacattatc
     5281 gggaactata aaggcattgg gaaaatcctg aagcagaatt tgccgaaaag cttcggccga
     5341 cattttatcg cgaaaatcac ccaccctaac tctgtagtaa ggttcgttga aagttaaata
     5401 agctccgtaa tccggataaa gttcttcgaa tcgttctttt attcgattag ccaatgtacg
     5461 ggcttgattg ccagattcga agtagatttg aatgcgatag ccttcgatgg taggttgaag
     5521 ttgattgatt tgaatatgcc tttgaataag agtatcgatc agagggtctt gtataatttc
     5581 gacctttcca ttgaattgtc cgaaagaatg tagaacgaaa gcggccaata aaagaataag
     5641 caatgataat tttttcgaca ttttgggctt aagcttagat tttagtgttg gaatgcgagc
     5701 tgagcactgg tactgaagat tccttgatga caattcgggc attgggcccg agtagagctt
     5761 ggctttcacc taattcttgc tcggtcatta tagatatcag atcggcttta atatttgtag
     5821 cgtaatctaa caaggtggca gtaatatttc ctgtttcata gacagttaat tgactcttta
     5881 cgttgtattt ttccagatat tttaatgctt cgtgggtatt ggcttctgtt ctcatgcgta
     5941 aggtgcgtag tttctcggtt agaagcgaaa gaacatgtac ttcggcttga aaaattcgag
     6001 caaattcaac tgtaattcct aacttttgca aagtatcagg cgaattgtcc accggcataa
     6061 tgattttctt gagagaaaat tttacaggag aaacaaatct aacggtaatc accggacaag
     6121 gtgcatgggt aactaagcga taggcattgc taccaatcca gaatttttcg aagccacttc
     6181 caccataggt tccggcaata atcagatcgg cttccttttg cctggccaga ttgcatactt
     6241 cggtgtatac ctttcctttt cgaagtttgt attcgagttt acctgaaatt tttttttgat
     6301 atttttcgat tatttctatc attcttgcct tggcttcttc tcgcatatcg ttactggacg
     6361 aagccataaa gggcaattgg tttacctgat tatcgaccca aacaagagta agattcgatt
     6421 tagctttaga ggcaactccc acggcatatt tcaaggcata gagtgccccc gccgagaaat
     6481 ctattggtac gatgatattt ttcataattc aggaatttac gctaagagac tacaatctaa
     6541 acgataaggc ctaagcacga aaggtaacat aaatagataa gccgatgagt ttttctgtat
     6601 gtataatcga tgaaaatcaa ttgtttgcat ttatctaatg atcataaaat tgataaggct
     6661 aatttaaggc aaattatatt agaaagaatg gaataggctt gaaaatgtta attttgctta
     6721 ggcgaaaaag tgagatgaat caaaatttgg atgcgagtgg aaagtcgttg actccggccg
     6781 aacgggaaat tgagaaggta ttacgaccct tggaattttc cgattttgcc gggcaaacgg
     6841 gtatagtaga taatttaaaa atttttgtgg cagccgcata tcaaagaggt gaggcccttg
     6901 atcatgtttt attgcacggc ccacctggtt tggggaaaac tactttatca tacattatag
     6961 ccaatgagct gggggtgggg attaaagtaa cctcggggcc tgttctcgag aaaccgggcg
     7021 atctggcagg attgctcact agcctgagac accgtgatgt tctttttata gatgaaattc
     7081 atcgtttgag ccctatggtg gaagaatatc tctactcagc catggaagat ttcaagatag
     7141 acatcatgat tgaaaccggt ccgaatgccc gtagtgttca aattcggcta aatcctttca
     7201 ctttgattgg tgctacaaca cgttcggggt tacttacggc tccgctccga tcacgttttg
     7261 gaatcaatgc aagacttact tattatacgg ccgatgtgct cgagaaaatt atccttcgct
     7321 cttcgggcat tttaaaagta aaaatcgatg aggaagctgc tattgaaatc gctcgccgaa
     7381 gcagaggtac acctcgcatt gccaatcttt tactgcgaag agttagagat tttgcgcaag
     7441 ttattggcga tggcgtcatc aatatgaaga ttgccactta tgccctgaat gccctgaatg
     7501 tggatagcca tgggttggat gagatggata ataagatttt gcgtacaatt atcgaaaaat
     7561 tcggcggagg gcctgttggc ttgaatacta ttgctacttc cgtatcggaa gatccaggaa
     7621 ctatcgaaga agtgtatgaa cccttcttga ttcaggaagg tttccttcag cgcacacccc
     7681 gaggtcgtga agctacggag cttgcctata aacatatagg taaactccct ccttcggttc
     7741 aaggtcgctt tttttaataa attggctttc atggatactt tatccataaa atcgaagatt
     7801 cgtcaacaag cttttgatat tggcttttcc aaatgtggct ttgcagttag tagattcctc
     7861 acctccgatt ttgaacatta taagaagcaa attgccctag gattccatgc cgatatgctg
     7921 tatctggaaa aagaggccga gaaaagattc catcctttac tcgttcatcc cgacattcgg
     7981 accgttattg ttcttttagc cggctataaa actaatgcac ggcaaaaaga tggagctttt
     8041 tattccattt cagattatgc tttgataccc gattatcata aaataatgaa agaaggcttg
     8101 caaaaattat cttgtttcat cgaaaatgaa tttcattcag atgttttaat aaattttgta
     8161 gatacaggtc atatttcgga aaaagcatgg gctcgtgagg cgggtttagg aaacttcgga
     8221 aagaacagta ttttgttaac ccccgaaggt tcttattttt ttattggtgt aattttgacc
     8281 gatctggtta ttatccccga tgaggccttt gatgaagatt tatgcaaaaa ttgcgattta
     8341 tgtataaaga gttgtcctac acaagcaata catcaacctt atgctgtgga tgcccgattg
     8401 tgcatttcgt atcataccat cgagaataaa ggccatattc cctccaatat tatagaaaag
     8461 ataggcaccc agatttatgg ttgcgatata tgccagaagg tttgcccatg gaatcgtcat
     8521 caaactgtga attctgtttt gttttcgcct ttgccacccc tattggatat gaaagccgaa
     8581 gatttcgaat ctgttacgga gactgatttt agggaaagat ttcatgaaag ccccatggtc
     8641 aggttaggac acagaagata catgcgaaat atagaggctg taaaaaatat ggccgctacc
     8701 gaaaggaagc ggccatatta aaaactgctc gttatttatc gaatcatgag tttttgtgtc
     8761 gctattccct gatctgtttt tatttctacc agatataaac ctggtttcaa atgattcagt
     8821 gaaatgggac tgctttccat ataattcatt tgagaaaaga cctccattcc cgtcgaattg
     8881 aaaattcgaa tagattgaat attggcattc gaggaacgaa tattcaaagc accgaaattt
     8941 cgacttagag ggttgggata aagaataatc tcggtggaaa ctgattgggg attgatgcca
     9001 gtataatcat atactgaaat atagtcattt cggataataa ttatagtaga atcagatgat
     9061 gatacttcga gcgtaacatc ataagttccc atggaaggat aaactatttt agggttgagt
     9121 tcggtcgatg ttgaaggctc tcctcctggg aaagaccata tacgcgacgt aaatgtagtg
     9181 ctggagagat cgaagaattc aaccgtatcg ttgattccga tagtagttgg aattccgacg
     9241 aagtttcccc agtaagggct tccgaagata tcgaaaaagg taagatattg tagcatcaag
     9301 tcctttttag ttgagggaga aacattatcg gtaagtcccc caaattcatt agaagaacca
     9361 atggtcttgt aaacagaacc aggatcattg gctatagtaa ctatataaga gggtgaagta
     9421 ttcataaata cggtaaatgc ttgatttatt ggttctatct tatcgatata gttgttgtcc
     9481 ccattgtaaa caaagctcat tccttcggta aatgtgcctg tttttccggc aattgtcgta
     9541 agcgtacctc cattgtcttt cagccctttg atttgaaaca ttggatgtaa aggagtagga
     9601 gtgctttgtt gatcataata ccaggtatct ccaccttcca tatatattct accaccttga
     9661 ttcaaatagt ttttaagcac aaggccttcg gcagcagaaa gaatgtgctt gttaggataa
     9721 actcctagac atacgaataa ggatttgtat ttacttaaat cggtggagaa ggaggtagta
     9781 tattcatttg cgacattgag ttgattcaat gcggcttgca tagttggccc ggagttgttg
     9841 ttgccatcaa gatcgatcac taaaacagga ataaggccaa ttgtcaaggt aaagtcatac
     9901 gaagcagtcc aaccaaaatc tcccatgagc gtcaaagtaa tcatggcctg ataacctgga
     9961 gggacggttg gaagagccga tatcaggaaa gcttgagatt gggaaccaag gggcaatata
    10021 tctccataag tagcttcctt cgtactaatt agaagataag gatctgcagt ggtgagagta
    10081 ccaatgagat tatatgctgc tgaagatcca atattgataa caggaatgtt cacataaaca
    10141 gattctcctg catcgaagcg ctgattgttg tttccaaggc tgtcgttaac acttatttct
    10201 ccctcctgta aaactggggc atgtgcctcg agcgaaaatt ggcttgtcca tgaattagaa
    10261 gaatcttgaa cgattaaagt gaagggtata acttggtggt cgggaataaa aggggatact
    10321 gataattgaa atccttcatc tacgctaata atctcgcctg caggtatagt gccatagttt
    10381 tcaatcgaat cggtaatttc gacataaggg ctaagagagc gtaaacttac ccatgtattg
    10441 acagatgaat ccaccccgat gtttttaacg gagatagtca ggttagatga ttcggaataa
    10501 tcgagcaaac cattaccatt gcctgttgta aaatcagcta tttgggtgct ttcataaata
    10561 acatagggac ctactgctgg aataacgggg atttgtgcaa ttaggggaat ataattatat
    10621 tcgactacag taagtgtaag cgtatcattg ggggtaatca aaggcgtaaa attaagagta
    10681 gccgttccgt tggctaccca agtggtattg agaatagtgc cattattcga aagagtaacc
    10741 cgggcgccat ttacattgca attgatagtg aaagtgcttg ctccaacgaa gatagtagga
    10801 ttgaaggttg ctgaaagagg ctgaggattt gcggttctga ccattaagct gggatcgcca
    10861 aatactgtcc atgtatcggc catattagaa ccatcggttc cgtaagaatc tatcattttg
    10921 gcacaaccat tcaaagataa gccagcaaaa gtccttctga tattgttagg ataggtttct
    10981 acgagtatgt ccaccatctc gtcttgtcct tccataggtg aattccagct ttggttgata
    11041 gtagaaccaa gaaatgagat agcacccgtt ggctggttat tttgagttgc tctgagccat
    11101 gcctctgcaa aacaggtagt attcataaat tcgccgttta cgcaagctac tgcccaaata
    11161 aaaggcaatt tacctacatt ggtgagctga tttacattgg cattgctgaa tcccgatgtt
    11221 ccccatgagg tagtacttcc atgaccggta tagagtataa tgctggttcc attgtttacc
    11281 tcgcttgaaa ccatagctgg agttggattt cctggagcat cattaccacc ctgcgaacca
    11341 tcgaataatt ccggattcca tgtataggta taacctaaca gatccgtttg catatttctt
    11401 acgtgctgat aatcatattc tccgtcgtca ccaggtcctt ggtcggaggc tatgccaagg
    11461 caagtagtat accagtcatc attaaggaag gaaggattct tttcgtattc gagggtgcgg
    11521 ctaacttgag tttgtacatg agccacattt tcggcagaaa aacgaccgat aaatacatcg
    11581 atataatggt cgttgcccac gatgtatcca taggcattat cgctgggtcc tcccaaccca
    11641 cttcctgtat tggtaggaat ttgaggccca tcgcctacca gcagtacgaa agttagagta
    11701 ttggtgttat aataattaac gatataattc ttaattacag ccgaagtact gccaatagtt
    11761 gcaacattca ccatttcggt agggatgcca atggagttct tccagtcgac ataaggttgc
    11821 atgctggcca taaaatcggg ataacaaatg acgagcatct tcccgaagtc gggtaggggg
    11881 gtataatcgg taggattgta attgaggaat tgtttttggt aaacttgtga gaattcctgc
    11941 tggattttaa tctggggtgt tttccgaagg taaggattca atccatggtc gccaatttta
    12001 taaatctcga ctctaagttc ggagaacact cttaaaactt tagtaaccgg attatagaaa
    12061 aatgggtaaa ctatgagggt ttggccacga aaatcgcgta atatataagg ctcacgcaat
    12121 tccccttctg tttgggggaa gaaatggttg gattgatatt caggtccata ggtatatggc
    12181 acagatgccg gatcgatgtc gcgagtaaaa tttcctttgc tcggagccac ttctataccc
    12241 gtgtaatcgg tataagaaga tgaaagaatt ctaattccca tcatagcttc atcggggatg
    12301 attacagaag cagttaactt gggaagatcg ggcgcaccgg ccgccagtat gcgagaagaa
    12361 ttgcccacct cgacaatatt ttcgatacct ctaggtgttt gtacaggctt cagatagaaa
    12421 cctttgatct ggaattcgat cacagatgaa gtaatatcag agctaatcag atgaatgcct
    12481 gctttctgtg gttcctcgga ggtcagaggt atccactcac cggctctgct cgccagcatg
    12541 caaaacaaca agccaaatag tagaagaaga cgtttcatgg ggtgtaaagt ttggtaattc
    12601 agtttaaaaa aatttcattc gacaaattta cattaatcct gttaattttt gaaaacatta
    12661 gggaataaaa aaccccgaga atggatctca gggttttaaa aggtaaagtt tgtaggatta
    12721 tttcattaag agggctttaa ccacaattga actggttcct gttgtaaatc gtatgtaata
    12781 gattccaggc ttgaaggagg tggtgttcaa ttgaatttga tacgatccgg cttctgattt
    12841 ttgttcaata agtggattag caatggtttg tcccagggag ttgaggattt cgatctttac
    12901 ttcagttgaa ttttgtaagg tataggagat attcaaaaga ttgctgaaag gatttggaaa
    12961 gacaaggaag aaagcatcct gtggaatttt tgttattccc actacttcga cataaagtga
    13021 attgctgggt gaagaaac
//
LOCUS       DAOORU010000052        12868 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_5996_length_12868_cov_4.830563, whole genome shotgun sequence.
ACCESSION   DAOORU010000052 DAOORU010000000
VERSION     DAOORU010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5996_length_12868_cov_4.830563"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PLF20_04840"
     rRNA            <1..310
                     /locus_tag="PLF20_04840"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            383..496
                     /gene="rrf"
                     /locus_tag="PLF20_04845"
     rRNA            383..496
                     /gene="rrf"
                     /locus_tag="PLF20_04845"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            658..1593
                     /locus_tag="PLF20_04850"
     CDS             658..1593
                     /locus_tag="PLF20_04850"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOX80366.1"
                     /translation="MKRKLSRFFKKYFTFSRREQRGVAVLLLLIGILLIVRYSLIKYS
                     KPEVIYYDQSSFTKDSIESSFPKDLAKETSKTKSYKKTTKPFEFNPNTLDEEGFKKLG
                     LPMWTIQTIMKYRNSGGKFYKPDDFARIYNLDAEDYERLKSYIRIPAQPEKHAESLKP
                     QFEKGKDVSKNIPVIEINMADSLLLQELPGIGPVYARRIIKYRQRLGGFVNAEQLLEV
                     YGMDTNRLSKIKPFVRIDTAYIRRIDLNHASFKDILRHPYMEYYLAKAIVNYRDKNNG
                     FRNVNELRQINLIYDDLYYRIRPYFEINPEYIPVQ"
     gene            1621..2994
                     /locus_tag="PLF20_04855"
     CDS             1621..2994
                     /locus_tag="PLF20_04855"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX80367.1"
                     /translation="MKIGKIIAILLLFSISESLEAQNKVSMKIDSLLDEYFAVSNSHR
                     ELALSLAENARKLAQKHGEKHNEAVALECLGVQYYYRGDYNKARDFLNQSLEIYNEEN
                     SSTGLASVFTNLALVEQDLGNLNQAHYYYTTSLVYDRNSHDSIGMAFTINNLGTLYLH
                     KGDFQRARAYFQKAYDIGKSVHHLEVIGNSIGNFGLLRLESSNPDSAIPYFRKALSIA
                     KQYGDEYAQAIANINLGLCMIEKSEADSAERYLTIAARTSEELEDNELQIECLLAKAK
                     LMLLLNQPELANRILFSAQKLNNPTERKKTSAQIFTLIGKNLLLMNQPEKARNYFLQS
                     LDIAKEIGALPEMIDAYKHLAMFYSAISMYDSCELYIDLYAKEWSKLTIDTTLPDTSF
                     VYEAKSNNKNILHRISKNSQLSPLKLLVTLVFALTILWAGMIYLPKILLSLRKKTKKD
                     NSNTSAK"
     gene            3046..3453
                     /locus_tag="PLF20_04860"
     CDS             3046..3453
                     /locus_tag="PLF20_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80368.1"
                     /translation="MKLKHSGFILFLATILIFSITSCEDETVQRPVNQYQVKYEIKNH
                     LKKKCNFYIRYFNPKFDNIVAEYYDSINEWSRELKGKTFDHLYFEAFTVRDSAMFEIS
                     IYVNGQQVVRDVDSCPGPSVCDTNRIKVSYVLQ"
     gene            3496..4950
                     /locus_tag="PLF20_04865"
     CDS             3496..4950
                     /locus_tag="PLF20_04865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX80369.1"
                     /translation="MGKGKIFRQFPSTFWAANVMELFERWAWYGIYMVLALYLTGSTD
                     TGALGFSQEQKGSLMGTVTFLLYLLPIITGAIADRYGYKKVLTIAFIILSTGYFSLAH
                     VRSYWSFYVLFLYLSVGAALFKPIITATVSHTTNDQTSSIGFGIFYMIVNIGAFLGPI
                     FASKLRILDWKFVFYLSSAIICINFILLFFFYKEPINKKQHTPILVSIKNIIKNTVTV
                     LADFKLMLFLIIMVGFWAMYLQLFYTLPIFIDQWMDTKILYDAIENFWPALAHIAGTS
                     QRTIAPEILTNIDAFYIVIFQLLVSSLVSRFSPLRAMTNGIMMATIGIALMFAFNNPI
                     YLFFSILIFSLGEMATSPKIQEYVGKIAPPDKTALYMGYSYLPLAGGHIVAGFISGSI
                     FAKIADKSTLLRNDLLSRGIVMPDISPTYTLNHFYSEASSLLHLDSSCLTSYLWQTYH
                     PGRIWILFSSIGLVSFLALLMYDRFFIRQKKILQ"
     gene            4950..5678
                     /locus_tag="PLF20_04870"
     CDS             4950..5678
                     /locus_tag="PLF20_04870"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOX80370.1"
                     /translation="MKKWFPSIFPALFITILMTGFLIFSNSSKISLIHLGIYPLSAKG
                     LLGIVLAPFIHENANHLYSNIIPFFVLTILLFYFYKDIAYEILGFSWIMTGILVWLGA
                     RPAYHIGASGVVYALMAFHVLSGMVRKNPRLLAISLLIVFFYGGMIWGIFPDFIPGRA
                     ISWESHLAGTITGSILAIYYRKEGPQRPLYSWELEPDEDDEDNEETKQNTPSQNLDKE
                     AEKAGEIMIKYIYKRKNTDEESSG"
     gene            5752..6855
                     /locus_tag="PLF20_04875"
     CDS             5752..6855
                     /locus_tag="PLF20_04875"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOX80371.1"
                     /translation="MKKNSLLSFPVLLAIMLFSCQKSDQYFLECNLEGLNDGMAYLQV
                     IGEDGIRTIDSTTISNGKFTFKGSQSEPIFAYITLNGKPDRIGLFLENARVSIYGHAD
                     SLEHIKISGSPLNDEYSKFKSMLASYENRQEELYLQYNQARKTNDTLALKNIETLWDL
                     LDKQQNKVIMQFISQHKHSVISPFLIHQRLVYNLNVNKLDSLTSIFPPELENSVYVRM
                     LKKRIQTLRNVEIGKPAPEIALDDTMGILRTLSSFRGKYVLIDFWASWCSPCREESPN
                     LVKAYQRFHDKGFEIFGVSLDTRRDKWVEAIRKDQLTWTHVSDLKGWKSEAAELYGVN
                     SIPHSVLVDPRGIIIQNNLRGKALIDFLEKLIK"
     gene            complement(7064..7915)
                     /locus_tag="PLF20_04880"
     CDS             complement(7064..7915)
                     /locus_tag="PLF20_04880"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HOX80372.1"
                     /translation="MLFSSLFAMGTAVLIWGERVLQVSDPSIIKDSPSLIAAYKYMQM
                     INHAGTFLLSGFIYLFIADKPRLKSLTSSKLPPQSQLWMVLLLIIISTPWISKVYEWN
                     RTFSLSYWPSIEQWLERTAQQSEDMVNVFLHRASFIGALANVLIIAVLPALGEELIFR
                     GILQPLLNQWWKNIHLSIMISALLFSMMHLDFFGFLPRLGLGIMFGYLYYFSANLWLP
                     ILAHFLNNFLALVVSFLSEKHITEIPYHEFGSVDNLWVNLFSFIFTLFIFAWFYKIRM
                     KTLPSKK"
     gene            complement(8002..10029)
                     /locus_tag="PLF20_04885"
     CDS             complement(8002..10029)
                     /locus_tag="PLF20_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX80373.1"
                     /translation="MAGKIVVDKFTKKETSKRAMRTKSDRSHLYILIFLAAFCLLLYA
                     NTIPNDYSLDDELVTWTNPQVSKGIKALPEIFTTFYYQASGNVGTIAFGYRPLAKATF
                     AIERSIFGVNPHISHFINILLYIATAWLLYLILRRLFRQSHWIFPLTITLIFLAHPLH
                     TEVVASLKNREELLAFLGALGGMWFFLDYANSGKIKHILLGTFCFLLGYISKSSIMPF
                     LLIYPLTLYFFTDVKSRQLVVITIVSLLVFIIAQLGPMLFLPEPIHSNALVENPLYLE
                     KNLMTRVATGFVGLLHYLGLLFWPSPLLFYYGYDMIPVASFANPVAFFSLILHLALLA
                     IAIVLFRKKHILSYAILFYLVSIAMYSNILVPVVGIVGDRFAYIASLGFAIAIGWIIF
                     RIVQGKSRKTISGYKPSAGIIILLLIILIPYCIVTIDRNKDWKNLTTLYEADIPYLER
                     SVKANTQYAGNLMYNAFNIAQKQGRLPSLENINKMIKHYNLSLKILPDYYDALNGLGT
                     VYSNFLGDYKRAIHYFEAAARSNPNNVAAYINLGYVYRELENNEKALECYHKVLAIDS
                     TKVKAYFKLAEIYFEMGDIATATRMNILATRFDSYTNVPYINIGNYHLLNRDTLDAVE
                     WYEKAVEKQPDYGLCMKLYMHYSKEGDLQKANYYRQMSEQTRNLTKKTFDK"
     gene            complement(10068..10862)
                     /gene="rsmA"
                     /locus_tag="PLF20_04890"
     CDS             complement(10068..10862)
                     /gene="rsmA"
                     /locus_tag="PLF20_04890"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOX80374.1"
                     /translation="MSKQDYNSQKVSPKKFLGQHFLHDKNIARKIVESLSLQTHNILE
                     IGPGMGMLTQYLLQKPNINLKLVEIDNESVVYLKERFPQIAENIIFSDFLQLDIHSIF
                     NEPFAIIGNFPYNISSQILFKVYEHVEMITELSGMFQAEVARRIISGPGNKDYGILSV
                     LIQTFYDGKYLFTVNENVFTPPPSVKSGVIHLKRNPGKHLPCPLEDFTLLVKTAFNQR
                     RKVLRNALSRFQFNFDAHVEVLLQKRAEQLNSQDFIYLTCSLQKFQ"
     gene            complement(10840..12684)
                     /locus_tag="PLF20_04895"
     CDS             complement(10840..12684)
                     /locus_tag="PLF20_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX80375.1"
                     /translation="MKRMNHFLFILPILLFCYWGVKGQDISKPPSKEQMAYNYYQQRN
                     FTKALPIFEELYNTRIGSNYYPYYLQCLIELKEFEKAKKTVETEMKRTPNQSRLQVDL
                     GYVYMVSGKTDKAYRIFEEVIENLKKNPQDAASLGIAFRAKNLNEFALKTYAKGRELL
                     KNPDAFQMEIASTYEASGDFHGMTEAFLDMLSTKPEQMSLIQSRLQFYLMQVPEKDNV
                     DYLYGAILERMQKNPSIMVYSEMMYWFTMMAGDYPEALRVALSLVKRNNEDGGRLLAV
                     ARNCIETNDEKTAEKALSYIYDQGSAHPLFLTAAEELYKLKYRKLIKGEHKKNESPEV
                     IAGQIEEILSQQGENMRTIELMRILARIYAYIMDRPEKALDILERAAGIRGLPPEILA
                     RINMDMAKVEVYIGRLWDAALIYGRLERDFPNHPLGEEAKLEYAKLMYQAGEHKWALL
                     HLEILRGAIHKPVANDAQELYLFIQEALNEDTTGRILHYFGSSELLSIRKQYAMALRT
                     LDSVSMFFGNSWGQDYALLRKARIYKEMREYNIADSIYSTLVRIFPRSLVADDALLEL
                     ASLREITHQPEKAYKTYEDLILNYPNSYMVETARKKLQILQNVKTGLQ"
BASE COUNT         4033 a         2624 c         2403 g         3808 t
ORIGIN      
        1 gctgggttca gaacgtcgtg agacagttcg gtccctatct gttgcgggcg tgggaagttt
       61 gaggggacct gaccttagta cgagaggacc gggttggaca aaccgctggt gtacctgctg
      121 tggcgcctag ctgcaccgca gggtagctat gtttggacga gagaagtgct gaaagcatct
      181 aagtacgaaa ctcaccccaa gatgagactt ccattgaggg cccttgtaga ctacaaggtc
      241 gataggctgc aggtgtaaag atggtaacat ccgtgccaag cagtactaat cgcccaatac
      301 tttatctcct gaacacaatc gctctattcg cacttcctcc tctctcctct cccattattg
      361 atattatcta tccctaaaat attatggcgg ctatggcgat ggtgttcacc tcttccctta
      421 ccgaacagag aagttaagcc catcagcacc aatgatactg cccatcaccg gacggaaaag
      481 taggccgccg ccaatatatc atagccaggc ataatacgcc tggctttttt tatatcctta
      541 acatcatatc attaatccct caatctgaat taatataaat acactatttt agcggttggc
      601 aataaaaaac tacatagcaa acaagaccga tagaaacgaa gaaaacagag attgacaatg
      661 aaaagaaaat tgtcgcgatt tttcaagaaa tattttacct tttcacgtcg agagcaaaga
      721 ggggtggcgg ttttactcct gctcatcgga attcttctta ttgtcagata ttctcttatt
      781 aaatattcga aacccgaagt catctactac gaccaaagtt cattcacaaa ggattcgatc
      841 gaatcctcat ttcctaaaga cctcgcaaaa gaaacatcaa aaacaaaatc ttataaaaaa
      901 acaacaaaac ctttcgagtt caatcccaat acattagatg aagaaggttt taagaagctt
      961 gggctaccta tgtggacaat ccagaccatc atgaaatatc gtaattcagg tggtaaattt
     1021 tacaaacccg atgacttcgc ccgaatatat aatctcgatg cagaagatta cgaacgattg
     1081 aagtcatata tccgtattcc agcccagcct gaaaaacatg ccgagagttt aaaacctcag
     1141 tttgaaaagg gaaaagatgt ttcgaaaaat attcctgtaa ttgaaatcaa tatggctgac
     1201 agtttgcttt tgcaggaatt acctggaatt gggccggttt atgcccgaag aatcattaaa
     1261 tatcgccaaa ggctgggagg tttcgtcaat gcagaacaat tgctcgaggt ttatggtatg
     1321 gatactaatc gcctatctaa aattaaaccc ttcgtgcgta tagatactgc atatatcaga
     1381 cgcatcgacc taaatcatgc ttctttcaaa gatatactcc ggcatcccta tatggaatat
     1441 tatctcgcaa aagccatagt aaattatcgc gacaagaata atggttttag aaatgtaaat
     1501 gaattacgcc aaatcaattt aatatatgac gatttatatt atcgcatacg accttatttc
     1561 gaaattaacc ccgaatatat ccctgttcaa taagaaataa attttgccaa taaaaaataa
     1621 atgaagattg gaaaaatcat agctattctg cttctgttca gtatttcaga atctctcgag
     1681 gctcaaaata aagtttctat gaagatcgat agtttgctcg atgagtattt tgcagtttct
     1741 aacagccatc gggagctggc tctgtcgcta gcagaaaacg ccagaaaatt ggcccaaaaa
     1801 catggagaaa aacataatga agccgttgct cttgaatgtt taggggtaca atattattat
     1861 cgtggagatt ataataaagc acgcgatttt ctcaatcaat cgttggaaat ctataacgaa
     1921 gaaaactcat cgacagggct cgccagtgtt tttaccaatc ttgccttagt ggaacaggat
     1981 ctgggaaatt tgaatcaggc acattattat tatactactt cgctggttta cgacagaaac
     2041 agccatgata gtataggaat ggcctttaca ataaataatc tgggaacact ctatctacat
     2101 aaaggagatt ttcaacgggc acgcgcttat tttcaaaaag cctatgatat aggcaaatcc
     2161 gtccatcatc tagaagtaat tggaaattcc atcggtaatt ttggtctgct tcgcctggaa
     2221 tcttcaaacc ctgattctgc tattccttat ttcaggaaag cattatctat agcaaaacaa
     2281 tatggagatg agtatgcaca agctattgca aacatcaatc ttggcctctg tatgatcgaa
     2341 aaatcagaag ccgattcggc agaaagatat ctgactatag ccgctcgaac ctccgaagaa
     2401 ctggaagata atgagttaca aatagaatgc ttattagcaa aggctaaact gatgcttttg
     2461 ctgaaccagc cggaacttgc caacagaatt ttattttcgg cacaaaaact aaataatccc
     2521 accgaacgga aaaaaacttc ggcacaaatc tttactctga tcggaaaaaa tcttttattg
     2581 atgaatcaac ccgagaaagc acgcaactat tttctgcaaa gcctcgacat tgcgaaagaa
     2641 ataggagcac tgcccgaaat gatagatgcc tataaacatc tggccatgtt ttattcggcc
     2701 atttcgatgt acgacagctg cgagctttat atcgaccttt acgcaaagga atggagcaag
     2761 ctgacaatag ataccacatt gcccgataca tcatttgtat atgaagcaaa atcaaacaat
     2821 aagaatatct tgcatcgtat atcgaaaaat tcacagctta gccctctaaa attactcgtt
     2881 acgcttgtct ttgccctaac tatcttatgg gcaggaatga tttatttacc taaaatactt
     2941 ctttcgttga ggaagaaaac aaagaaagac aattcgaata cgtcggccaa atgattacat
     3001 attcaaaacc ttttctattt ttgtagctgc aaacctattt caactatgaa actcaaacat
     3061 tccggtttta ttctcttttt ggcaaccatt ttgatattca gtataacatc ctgtgaagat
     3121 gaaacagttc aaaggcctgt aaatcaatat caggtaaaat atgaaatcaa gaatcatctc
     3181 aaaaagaaat gcaatttcta tatacgctat ttcaatccaa aattcgacaa tattgtggca
     3241 gagtattatg actccatcaa cgaatggtcg agggagttga aaggaaaaac attcgaccat
     3301 ttatattttg aggcatttac ggtaagggat agcgccatgt tcgaaatcag tatctatgta
     3361 aatggtcagc aagtggtgag agatgttgac agttgtccgg ggccatcggt atgcgataca
     3421 aaccggatta aagtctctta cgttctgcaa tagatacgta aaaccctctt tatcagctaa
     3481 agaagggtaa aaaagatggg caagggaaaa atatttcggc aatttccatc aactttctgg
     3541 gcagccaatg tcatggaatt gttcgaacgt tgggcatggt atggtattta tatggtttta
     3601 gccctctatc tgacaggttc gaccgatacg ggtgccttag gattcagtca ggaacaaaaa
     3661 gggagtttga tgggtactgt gacctttttg ttatatctgc tacctatcat caccggagcg
     3721 attgccgaca ggtatggcta taaaaaagtt cttactatag cttttatcat tctaagcact
     3781 ggatatttca gtctggctca cgttagaagt tattggtcat tctatgttct tttcctttat
     3841 ctctcggtag gagcagctct attcaaacct atcataacgg cgactgtttc gcatacaacc
     3901 aatgatcaga cctcatctat cggatttgga attttttata tgatcgtaaa tatcggtgcc
     3961 tttttgggcc ccatctttgc tagcaaactt cgcatactcg attggaaatt cgtcttctat
     4021 ctttcatcgg ccattatttg catcaatttt atcctattat tctttttcta caaggaacca
     4081 attaataaaa aacagcatac tcctatactt gtatctatca agaacatcat caagaatacg
     4141 gtaacggttc tggcagattt caaactaatg cttttcttaa taattatggt aggcttttgg
     4201 gcaatgtatt tgcagctgtt ttacacttta cccattttca tcgatcaatg gatggataca
     4261 aaaattctat atgatgcaat cgaaaatttc tggcctgctt tggcgcatat tgcaggaaca
     4321 tcgcaaagga ccatagcccc tgaaattctt accaatatcg atgcattcta tatcgtgata
     4381 ttccaacttc tggtttcgag cctggtaagc cggttttcgc ctctcagggc gatgaccaat
     4441 ggtataatga tggcaacaat aggaattgcc ttgatgtttg cctttaataa tccgatttat
     4501 ctttttttta gtatattgat ctttagtttg ggtgagatgg ctacttctcc aaaaattcag
     4561 gaatatgttg ggaaaatagc cccacctgat aaaacagctc tctatatggg atacagttat
     4621 ctgccacttg ccggcggcca tattgtagct ggcttcattt cgggctctat atttgcaaaa
     4681 attgccgata agtccactct actacgtaac gatttacttt ccagaggaat cgtcatgcca
     4741 gatatctctc ctacttatac cctaaaccat ttctactccg aagcctcatc attgcttcat
     4801 ctcgactcat catgccttac ttcttacctt tggcaaactt atcatcccgg tcgtatctgg
     4861 atattatttt catctattgg tttggtttcg tttttagcct tgcttatgta tgataggttt
     4921 ttcataaggc aaaagaaaat tctacaataa tgaaaaagtg gtttcccagc atcttccctg
     4981 ccctattcat tacaatattg atgacgggtt tccttatatt ttcgaattca agtaaaatct
     5041 cgttaattca tctgggaatt tatcccttaa gcgccaaagg acttttaggt atcgttctgg
     5101 ctccttttat ccatgaaaat gccaaccatt tgtattccaa cattattccc ttttttgtac
     5161 tgaccatact cttgttttat ttttacaagg atattgccta tgagatttta ggtttttcat
     5221 ggatcatgac gggtatactg gtttggttag gggcacgtcc ggcttatcac attggagcaa
     5281 gcggagtggt gtatgcctta atggcatttc atgtcttgag cggaatggta agaaagaacc
     5341 cacgcttgct ggccatttca cttcttatag tcttttttta tggaggaatg atctggggca
     5401 tttttcccga ttttattcca ggccgggcta ttagctggga atcacatctg gcgggcacga
     5461 ttacaggttc catactggct atatattacc gcaaggaagg gccacaaagg ccattatata
     5521 gctgggaact cgaacccgat gaagacgatg aggataatga agaaaccaaa caaaatacac
     5581 catcccaaaa tctcgataaa gaagccgaga aagccggaga aatcatgata aaatatattt
     5641 acaaaagaaa aaataccgat gaggagagtt ccggctaaac catcgaaaat gtaaatccac
     5701 tttgctaatg cacaaaattt tataatttcg tggcaaccaa aaacaattcg aatgaaaaaa
     5761 aattcactgc tttcgtttcc ggtcttattg gcaatcatgc tatttagctg ccaaaaatcc
     5821 gaccagtatt ttctcgagtg caatctcgag ggtttgaatg acggaatggc ctacttgcag
     5881 gttattggag aagatggtat tcgaacaatc gactcgacaa ccatatcgaa tggaaaattt
     5941 accttcaaag gttcacaatc cgaacctatt tttgcctata tcactttaaa tggcaaaccc
     6001 gaccgcatag gccttttcct ggaaaatgcc agagtctcga tatatgggca tgccgacagt
     6061 ttggaacata taaagatttc gggttcaccc ctaaatgatg aatattcaaa attcaaatct
     6121 atgctggcat cctatgaaaa tcgccaggaa gaattgtatc tgcaatacaa tcaagcacgt
     6181 aaaaccaatg ataccttggc actgaaaaat atcgaaacac tgtgggattt actcgacaag
     6241 cagcaaaata aagtgattat gcaatttatc agccagcata agcattcggt catttctccc
     6301 tttttgattc atcaaagact ggtttacaat ttaaacgtaa ataaactcga cagccttact
     6361 tcgatatttc ctcccgagct cgaaaactcg gtgtatgtac gcatgttaaa gaaacgtatt
     6421 caaaccttac gcaatgtcga aattgggaaa cccgcaccag aaatcgctct cgacgatacc
     6481 atgggcattt tacggacttt gtcctctttc agaggaaaat acgtcctgat tgatttttgg
     6541 gcatcatggt gtagcccatg ccgagaagaa tcgcccaatc ttgtaaaggc ctaccaacgt
     6601 tttcatgaca agggattcga aattttcggc gtatcactgg atacacgtcg cgataaatgg
     6661 gtagaagcta tcaggaaaga tcaactgaca tggacacacg tttcagacct taaaggctgg
     6721 aaatcggaag cagcagaact ttatggggtt aattccatac ctcactccgt tctggtcgat
     6781 ccccggggaa ttatcataca aaacaatttg agaggtaaag ccttgatcga ttttcttgaa
     6841 aagctgatta aataaaagat gtctccatga tagtgcgttt catttcaaag taatgtccat
     6901 cgactatcta aaatccatca tcttagataa ggttatgaaa gcgaaataaa tcagaaagac
     6961 tacttgtcag aatttcaggc cttatcctac ataataaatg attggcttaa acttaatagc
     7021 actgaaatat catcaggggc tcccgattcc gattttcaca atctcacttc tttgagggca
     7081 gggtcttcat tcgaatttta tagaaccatg caaaaatgaa aagcgtgaat ataaaactga
     7141 acagattgac ccataaattg tcgacacttc caaactcatg ataaggaatt tctgtaatgt
     7201 gtttttcaga aagaaaagaa accaccagag ccaggaaatt attgaggaaa tgagcaagaa
     7261 taggcaacca caggttagcc gaaaaataat aaagatatcc gaacatgatt cccaatccca
     7321 ggcgaggtaa gaacccgaag aaatcgagat gcatcatact gaatagcaaa gcagatatca
     7381 ttatactcaa atgaatgttt ttccaccact ggttcaacag gggttgcaag attcctctaa
     7441 agataagttc ttcgcccaag gcaggaagta cagcaattat gagaacattg gccaatgcgc
     7501 ctataaatga agctctatgc aaaaaaacat tcaccatatc ctcgctttgc tgcgcagttc
     7561 gttccaacca ctgttctatg gaaggccaat aagacaagga aaatgttcga ttccattcat
     7621 acactttcga aatccatggc gtagaaataa tgatcagcag aagtaccatc catagctgtg
     7681 actggggtgg caatttgctg gaggttaggc ttttcagtct cggtttatca gcaataaata
     7741 aatagataaa acctgagagg agaaaagttc cagcatgatt gatcatttgc atatatttat
     7801 aggcagcaat caacgaaggg ctgtctttga taatcgatgg gtctgatacc tgaagaaccc
     7861 tttcacccca gataagtaca gccgttccca ttgcaaacaa cgatgaaaac aacatgcaaa
     7921 tcattatgat tatcatcaat aataaaagct tcggaaaagg cttttgatga gaaaaatcgg
     7981 gaataaaact gaaatgcatt tctatttatc gaaggttttt tttgtgagat ttctggtttg
     8041 ctcagacatt tgcctataat aattagcttt ctgcagatcc ccctctttcg aataatgcat
     8101 gtaaagtttc atacataatc cataatcggg ttgtttttcg acggcttttt cgtaccactc
     8161 cactgcgtcc aatgtatctc gattgagcaa atgataattt cctatattta tgtaaggtac
     8221 attagtatat gaatcgaaac gagtagccag tatattcatt cgagttgccg ttgcaatatc
     8281 ccccatctcg aaataaattt cagcaagttt gaaataggct ttcactttgg ttgaatctat
     8341 agcaagaacc ttatgataac attcaagggc tttctcatta ttctccaatt ctctgtatac
     8401 ataaccaaga ttgatataag cggccacatt attggggtta gatcgggcag cagcttcgaa
     8461 gtaatgaatc gcccgcttgt aatctcccaa gaaattgcta tacacagttc ccagtccgtt
     8521 caaggcatca tagtaatcgg gtaagatttt caagctcaaa ttataatgct taatcatctt
     8581 gttgatattt tcaagtgaag gaagtcgacc ttgtttttgt gcaatattga aggcattata
     8641 catcaaattg cccgcatatt gcgtgttggc ttttaccgaa cgctccaaat acggaatatc
     8701 ggcttcgtag agagtggtaa gatttttcca atctttgttt ctatcgatcg ttactatgca
     8761 gtagggtatc aagattatca aaagtagaat tatgatacct gccgagggtt tgtaacccga
     8821 aatggttttt cgtgattttc cctgaacaat tctgaagatt atccatccta tagcaatagc
     8881 aaatcccaga gaagctatat aagcaaaacg atctccaact attccaacta caggaacgag
     8941 aatattggaa tacatggcaa tactgacaag ataaaacagt atggcataag aaaggatatg
     9001 tttcttgcga aatagtacaa tggctatggc aagtaaagcc aaatgaagga ttagagaaaa
     9061 aaatgctacc ggatttgcga aacttgccac gggaatcatg tcatagccat aataaaaaag
     9121 aagaggagaa ggccagaata aaaggccgag gtaatgcaac aaacccacga atccagtagc
     9181 tacccttgtc atcaggtttt tctccagata aagtgggttc tccaccaaag cattagaatg
     9241 aatgggttcg ggtaaaaaca acattggtcc taactgagca atgatgaata cgagcaaaga
     9301 aactatggtt atgactacca attgcctcga tttaacatca gtgaagaagt atagcgtaag
     9361 aggataaatc aataagaatg gcataatgga cgacttggag atataaccca ataagaaaca
     9421 aaaggttccc aacaagatat gctttatttt tccgctgttg gcataatcga ggaaaaacca
     9481 cattcctccc aaagcgccca aaaaggcaag caattcttct ctattcttca gactggccac
     9541 tacttcggta tgtaaaggat gtgccagaaa aataagtgta atagttaaag gaaaaatcca
     9601 atgagattgc ctgaaaagcc ttcgaagtat aaggtaaagc agccaggcag ttgcaatata
     9661 aagcaaaata ttgatgaaat ggcttatatg agggtttact ccaaaaatag aacgttctat
     9721 agcaaacgta gcctttgcta aaggcctgta accaaaggct atggtcccaa cattcccact
     9781 cgcctggtaa taaaatgtcg taaatatctc gggcaaagcc tttatccctt tcgatacctg
     9841 agggttggtc caggtaacaa gttcatcatc cagagaataa tcattgggta tagtattcgc
     9901 ataaagaagc aaacaaaaag ctgcgagaaa aatcaaaata tataaatgac ttcgatcgct
     9961 ttttgtccgc attgctcgct ttgaagtctc tttcttcgta aatttgtcta cgacaatttt
    10021 tccggccatt ttctttcagt aggtaaaaat attataatta taacatatca ttgaaatttc
    10081 tgcaatgagc atgtcaaata aataaaatcc tggctattga gttgctcggc acgtttttgc
    10141 aacaatactt cgacatgcgc atcgaaatta aactggaatc tcgaaagtgc attgcgcaga
    10201 acttttcttc tttggttgaa ggccgttttg accagtaggg tgaaatcctc gagcggacat
    10261 gggagatgtt tgccaggatt gcgtttcaaa tgaatcacac ccgatttcac tgacggtgga
    10321 ggagtaaata cattttcatt taccgtaaaa agatatttcc catcgtaaaa tgtttgtatt
    10381 aaaacactta aaatcccgta atccttgttt cccggaccgc tgatgatgcg cctagccact
    10441 tcggcctgaa acattccgct aagctcggtg atcatttcta catgttcata aactttgaaa
    10501 agaatttgtg aagatatatt atagggaaaa tttcctatga tggcgaaagg ttcattaaat
    10561 atactatgaa tatcgagttg aagaaaatcg gaaaagatga tgttttcggc tatttgggga
    10621 aatctttctt tgagataaac taccgattca ttgtcgattt cgacgagttt gaggtttata
    10681 tttggctttt gcaataaata ttgggtcaac atacccatgc ccgggccgat ctcaagaatg
    10741 ttatgcgttt ggaggctcaa actctctaca atcttccggg caatgttctt atcatgtaag
    10801 aaatgctgtc cgagaaactt ttttggactt accttttggc tattgtaatc ctgttttgac
    10861 attttgcaga atctgaagtt ttttgcgagc agtttcgacc atataactgt ttggatagtt
    10921 taaaatcaag tcctcataag ttttataggc cttttcgggt tgatgggtta tttctcttaa
    10981 ggaagcaagc tctaatagag catcgtcggc aaccaggctt cgaggaaaaa ttctcacaag
    11041 agtagaataa atggaatcgg ctatattgta ttccctcatt tccttataaa tccttgcttt
    11101 tctcaacaat gcataatcct ggccccagct atttccaaag aacatcgaaa cgctatcgag
    11161 cgttcgaagt gccatggcgt attgttttct aatactaagc aattcactgc tgccaaagta
    11221 atgcaatatc cgaccggtgg tatcctcgtt caaagcctcc tgtatgaata aatacaattc
    11281 ctgggcatcg ttggccacag gtttatgaat agctcccctt agaatttcaa gatgaagcaa
    11341 agcccattta tgctccccag cctgatacat tagtttggca tattctaact tggcttcttc
    11401 tccaagggga tgattgggaa aatctctttc caaacgtcca tagattaaag cagcatccca
    11461 caaccgtcct atataaactt caacctttgc catatccata ttgattctgg ccaatatttc
    11521 aggcggcaaa cctcttattc cggccgcacg ctcgaggatg tcaagagctt tctctggccg
    11581 gtccattata taagcatata ttctggctaa aatcctcatc aattcgatgg tgcgcatatt
    11641 ttctccctgc tgtgacagaa tctcctcgat ttgccctgca ataacctcgg gtgattcgtt
    11701 tttcttatgt tctcctttga tgagttttcg gtatttcaac ttatacaatt cttcggctgc
    11761 agttaaaaat aatggatgag cagaaccctg atcatatata tatgacaaag ccttttcagc
    11821 agtcttttca tcgtttgtct caatgcagtt tcgggctacg gccaataacc ttcctccatc
    11881 ttcattatta cgttttacga gtgataatgc taccctcaga gcttcaggat aatcacctgc
    11941 catcatcgta aaccaataca tcatctcgga ataaaccata atggaaggat ttttttgcat
    12001 tctttccaaa attgccccat ataaataatc cacattatcc ttctcgggta cctgcattag
    12061 ataaaattgc agcctcgact ggataagact catttgctcg ggctttgtgg aaagcatatc
    12121 cagaaatgct tctgtcattc catgaaaatc accactggct tcataagtgc tggcaatctc
    12181 catctgaaaa gcatccggat ttttcaataa ttctctccct ttcgcataag tttttaaagc
    12241 aaactcattc aaattttttg ccctgaacgc tatgcccaat gaagcagcat cctgaggatt
    12301 tttctttaaa ttctcaatga cttcctcaaa tatcctatat gccttgtcgg ttttacccga
    12361 aaccatataa acataaccca aatcaacctg taatctcgat tgattgggcg ttcttttcat
    12421 ctcggtttct accgtttttt ttgctttttc aaattccttc aactcgatca ggcactgcaa
    12481 ataataagga taataattgc tcccgattct ggtattgtat aattcttcga aaataggaag
    12541 tgctttggtg aaatttcttt gctgataata attataggcc atctgttcct tcgaaggagg
    12601 cttgctgatg tcttgacctt tcactcccca ataacaaaaa aggagaatag gcagaataaa
    12661 aagaaaatga ttcatccttt tcatgcagca aatttagtct gatttcattg ccagagcaat
    12721 gccataactc cttattcgag gaatccgggg caataacgca agactcttat cgaaaatata
    12781 taaaatgtat aggaatccct ctatatcatt atcttcctga cttcgacaat gcgtcaccct
    12841 aaaaattttt ccaaaacttt tcatcaaa
//
LOCUS       DAOORU010000053        12795 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_6057_length_12795_cov_5.680141, whole genome shotgun sequence.
ACCESSION   DAOORU010000053 DAOORU010000000
VERSION     DAOORU010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12795
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6057_length_12795_cov_5.680141"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PLF20_04900"
     CDS             <1..1270
                     /locus_tag="PLF20_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HOX80376.1"
                     /translation="LIFVRKNIEIMKLDRRIFFKAFSLLVALSIILGLSFGCNRARQA
                     DEKPYLVVLSLDGFRWDYPQYFSTPNLDKMAQEGVHAHSLIPCFPTKTFPNHYSIATG
                     LYPDHHGIIQNSFYVPAIGTYEPTNRQSIMNGAFYEGEPIWLTAENQGMKSACCYWVG
                     SEAPIKGKYPSIWLRYNQDLPFTSRIDSVIAWLQLPSEKRPHLIMWYMHQPDKIGHHE
                     GPLAASTGRMVSYLDSLVGVFRARLNELPIAKKVNFVVVSDHGMAKVNESNNLYVDQL
                     VPRYWITMACGGEVQMNLDGIPAYKDSLYQRLKKVNHLTVWKKEEVPQRLHYSTNPRI
                     YDLVVLADTGWNIMLTHPAKILPGHHGYDNVYPDMHGIFYGVGPAFKNGYERPSFENI
                     HLYPLFAHILKLKPAVVDGRLKEVEDILKD"
     gene            complement(1267..2028)
                     /gene="truA"
                     /locus_tag="PLF20_04905"
     CDS             complement(1267..2028)
                     /gene="truA"
                     /locus_tag="PLF20_04905"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOX80377.1"
                     /translation="MDNQRYFIHLAFDGTNYCGWQRQKNATSIQQEIEEKISILLKKP
                     IDITGCGRTDTGVHASSFYAHFDFDPMPKQQLIILSYRLNHILPLDIAISEILPVKPE
                     AHARYDALWRTYRYYISLRKNPFTRNYRWFRSQCPDIEKMNQLAEILLAVKNFTSFSK
                     LHSDTENNLCTLTQAFWEIRKDELVFTITANRFLRNMVRAIVGTLWQLGKEENAVDHL
                     REIIAKRDRSAAGPSVNPHALFLEEIIYPDDIFLV"
     gene            complement(2031..2282)
                     /locus_tag="PLF20_04910"
     CDS             complement(2031..2282)
                     /locus_tag="PLF20_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80378.1"
                     /translation="MKTTILGLKITQRAEKSTEVQDVLTKFGCSIHTRLGLHEASEDY
                     CSQEGLIILELVGDVGEMLKLEEALKQIPGVEVQKMVFD"
     gene            complement(2336..2992)
                     /locus_tag="PLF20_04915"
     CDS             complement(2336..2992)
                     /locus_tag="PLF20_04915"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HOX80379.1"
                     /translation="MDNKSTGIIWDWNGTLLDDVSLCIDVMNSLLNDRHLPLLNHERY
                     REIFDFPVIEYYKKLGFNFNVDPFEVIGLEFMKRFLEHIEKVPLMDGAVEILEWFDNK
                     GYRQYILSAMEQQSLDKILLDKGIHPYFEAISGINNHYGGGKMEEGRHFITDNKMNPN
                     EAYLFGDTIHDFEVGDRLGMKVVLIAQGHQSKQRLIETKAVVVDNFEQLKKWFEINGK
                     "
     gene            complement(3225..3734)
                     /locus_tag="PLF20_04920"
     CDS             complement(3225..3734)
                     /locus_tag="PLF20_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOX80380.1"
                     /translation="MGKEAIKILSVDRDELLKMLNAALAEEWLAYYQYWIGARLMEGP
                     MRAEIEPELLLHATQELNHAVLLVNRIIQLEGTPILNPNDWSKLAHCQYEEPKDPYIE
                     VILDQNLKGERCAIERYEQIANFTSGKDHATYQMAVQILNEELEHEHDIQDWINDIHR
                     MKEEWKKLR"
     gene            complement(4044..4117)
                     /locus_tag="PLF20_04925"
     tRNA            complement(4044..4117)
                     /locus_tag="PLF20_04925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4081..4083),aa:Arg,seq:tct)
     gene            4741..7575
                     /locus_tag="PLF20_04930"
     CDS             4741..7575
                     /locus_tag="PLF20_04930"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOX80381.1"
                     /translation="MDSRRCFYFLEIFIFSFFRLQAQIIHTEPVFPLVNQSVIIYYDA
                     AEGNQGLMGYTGDVYAHTGVITNLSTSPSDWKYVKTNWGQNTQETKLERISANYYRLT
                     ISPDIRSYYGVPANEQILKMAFVFRSGVTIGGSYKEGKDVGNADIFVDVYPPDLSINI
                     TSPQGNHLIEAEGDTILVHAISPQADSMWVFENGSLRKAVAGVFMDDTLVASPINGYW
                     NDHHLIFVAKNSQQIVRDTLFYVVIPQPTVVELPDGIYDGINYVDDSTVILCLQAPGK
                     KNVFAIGDFSNWEILPEYYMNKTADGERFWLEISSLIPAKEYIYQYLVDGKIRISDPY
                     CDKISDPWNDKYIPTSVYPAMLIYPSGKTTGIASVLQTAQEPFAWQIEQFSPPSREKL
                     VIYELLIRDFTTKHTYQSVIDTLNYLVKLGVNAIELMPLMEFEGNSSWGYNPMTYFAP
                     DKYYGPKNDLKKLIDECHKRGIAVILDIVLNHAYGNNPLVKLYWDDVKQIPAADNPWF
                     NQTSPNPSYSWGYDFNHESPYTKQFVYRVTRYWLTEYKADGFRFDFSKGFTNKPGDGW
                     AYDATRIAILKNYTDSIWSVNPQAYVILEHFTDNLEEKILANYGMLLWGNLNGAYCDA
                     SMGYTSSSDFSSIDYQRRGWNNPHVVGYMESHDEERLMYKNLTWGVSTNPDYDIKDMT
                     IALERAALAATFFIPVPGPKMIWQFGELGYDYSIDYNGRVGEKPVRWDYYTDWRRHYL
                     YEVYSALHYLKNNYPVFNTTDYSYKLNGVDRRIILRHSDMDVVITGNFDVNAKDIVPG
                     FTRDGKWYEFFSGDSLEVQNLTATITFEPGEYHLWSTSRIPKPASLNTGFVEMEEQPS
                     FRIWPNPANEKIFVSFYLEQRSQVELVLYNISGQKIGQRILGRIEAGSYTKEVPLSDI
                     ALQALETGIYIVELKTNNRIQYSKLAIGVY"
     gene            complement(8080..9858)
                     /locus_tag="PLF20_04935"
     CDS             complement(8080..9858)
                     /locus_tag="PLF20_04935"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HOX80382.1"
                     /translation="MRCRRIFDLLEYNVERYHQPIALAGKKAGKWITYSSEEYAQYAN
                     WVSYGLMAMGVRKEDKISTISTNRPEWNFVDMGILQIGGVHVPIYPTISDDDLMYILK
                     EAEVKLIFVGNKYLYGKIRDFSEQLPSLIAIISFDEVEGCIPFSEFIHFAKSNIQKDL
                     LDRLKATVSPYDLASIIYTSGTTGLPKGVMLTHENHLCNSLVAAFTINLKSTYTELSF
                     LPLSHSYERMVNYATIYLGMPVYYADVTANILLNFQEIKPQLMITVPLLLEKVYQGIL
                     KKRLELGRIQRFVFDWALSLAQRFEINKDLGKWYRWKLSIADRLVFVKWREALGSSLV
                     KIVTGGASIRPELVNIFWAAGIKVYEGYGLTEASPLVAFNTESAIKVGTIGKAIPDIL
                     VKISDEGELMVNGPNVMKGYYKHPELTNEVIEPDGWLHTGDIATIDEEGFITITGRKN
                     EIFKMASGIYVNPEKLENKLQQSFFISQAFVVGEHQSFLSAIILPEFDYIKEYFAHQG
                     IYFNSNLEIIQNQKVISLIEEEIKILNGPGIKENESIQRFALVADDWTIETGELSPSL
                     KLKRRVVMEKYKELIQNFYRKENGGW"
     gene            complement(10149..10958)
                     /locus_tag="PLF20_04940"
     CDS             complement(10149..10958)
                     /locus_tag="PLF20_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOX80383.1"
                     /translation="MPFMSFQNHPVYYESRGLGPAIVFLHGFMESHKIWESFANSFSN
                     EFQSITFDLPGHDQSDIIAPVHGMELMADCTKSILDHLGVKDCILVGHSMGGYVSLAF
                     ADKFPERVKGLVLFHSSALPDSDEGRKNRERTIEFIQQNKTGFILQFIPSLFAPSNAE
                     KYQMEIQMLIESASYMKPQAIIAAQIGMKERPSYLDVLTHIQAPVLFIIGKQDSRISA
                     KDYFSQIFLPSHSEILVLENVGHNGYIEAFPVTSAAIRHFAQRAFELYSAI"
     gene            complement(11247..11711)
                     /locus_tag="PLF20_04945"
     CDS             complement(11247..11711)
                     /locus_tag="PLF20_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOX80384.1"
                     /translation="MARFKKSEGRSTPGINTGSMSDIIFMFLFFFMVITTIRESSLIV
                     RVKVPQATEVAKLERKSLVSFIYVGQPIDYKYGTESRIQLNNQFASVADIREFITRER
                     EARDEVDRPLLTTSLKVDEETRMGIVTDIKQELRQVGAFKINYSARKTLAKE"
     gene            complement(11714..12286)
                     /locus_tag="PLF20_04950"
     CDS             complement(11714..12286)
                     /locus_tag="PLF20_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOX80385.1"
                     /translation="MKRARPELNTGSMADISFLLLTFFLLTSSIDTDLGILRRLPPPP
                     DPTQEQVDIKKRNIFTVLVNKNDELLVQGRPMDISMLRAAAKEFLANPYNRPDLPEKK
                     MEIIPGLGEVETSKGVISLKNDRGTSYKMYIKVQNELTAAFNELRDELSMERFGVKFS
                     QLSNQQYIDAIQKAIPMSISEAEPENVGGK"
     gene            complement(12307..12639)
                     /locus_tag="PLF20_04955"
     CDS             complement(12307..12639)
                     /locus_tag="PLF20_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80386.1"
                     /translation="MSSTLIDIGIYITYALALVALVSVIVFPLMSVFKDFKQAKGGLL
                     GFLGIIALIVIAILLSPADQGPFYDKFSIGPTLSKAIGGTIIATYILFGGLILSILYF
                     EVVKWFKK"
     gene            complement(12646..>12795)
                     /locus_tag="PLF20_04960"
     CDS             complement(12646..>12795)
                     /locus_tag="PLF20_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004335089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOX80387.1"
                     /translation="TTVFGLIVALILQVFYNYLLSKVESIVNDMEDSTISFMDILVRY
                     KNTKK"
BASE COUNT         3804 a         2489 c         2540 g         3962 t
ORIGIN      
        1 ccttattttt gttcggaaaa acatagaaat catgaaactc gaccggcgta ttttttttaa
       61 agcgtttagc ctgcttgttg cactatctat aattttaggg ttatctttcg gctgcaacag
      121 ggcgcggcaa gccgatgaga aaccttatct ggtggttctt tcactggatg gtttccgatg
      181 ggattatcct cagtattttt ctactcccaa tctcgataag atggcccaag aaggggtgca
      241 tgcgcattct cttattcctt gtttccctac caagactttc cccaaccatt attccattgc
      301 cacgggctta tatcccgacc atcacggaat tatacaaaat tcattttatg tacctgcgat
      361 tgggacttat gaaccaacta accggcagag cattatgaat ggagcatttt atgaaggcga
      421 gcccatctgg cttacagcag aaaatcaagg catgaagtcg gcttgttgtt actgggtagg
      481 cagtgaagct cccatcaagg ggaaataccc atctatttgg cttcgctaca atcaggattt
      541 gccttttacc tcccgcatcg attcagttat tgcatggttg caattgcctt ccgaaaaacg
      601 acctcatctg atcatgtggt atatgcacca acccgataaa attggccatc atgaaggacc
      661 gcttgcggct tcgaccggca gaatggtttc ctatctcgat tcactggtgg gcgtttttcg
      721 tgctcgactc aacgaattgc ccatagcaaa gaaagtaaac tttgttgtgg tttccgacca
      781 tggaatggca aaagttaacg aaagcaataa cctgtatgtg gaccaacttg tgcctcgtta
      841 ctggatcacc atggcctgtg gtggggaagt tcaaatgaat ctggatggta ttccggccta
      901 taaggattct ttatatcaac gtttaaaaaa agtaaaccat ctcacggttt ggaaaaagga
      961 agaagtgcct cagcgtttgc attatagtac gaatccccgt atttatgatt tagttgtttt
     1021 agcggataca ggttggaaca tcatgcttac tcacccggca aaaattcttc ctggccatca
     1081 tggttacgac aatgtttatc ctgatatgca cggaattttt tacggagtgg ggcctgcttt
     1141 caaaaatggt tacgaaaggc caagtttcga gaatattcat ctgtatccct tatttgctca
     1201 tattttgaaa ctaaaacctg cagtagtaga tggtcggctc aaagaagtag aggatatatt
     1261 gaaagattaa acaaggaaga tatcatcggg ataaatgatt tcttcgagga aaagagcatg
     1321 agggtttaca gaaggcccgg cagccgaacg atctcttttg gctataattt cacgaaggtg
     1381 atcaacggca ttttcttctt ttcccaattg ccagagcgtg cctacaatgg ctcttaccat
     1441 gttgcgtaag aaacggttgg ctgtgatggt aaaaacaagc tcatcctttc tgatttccca
     1501 aaaagcctga gtaagcgtgc aaaggttatt ttcggtatcc gaatgaagtt tggaaaaact
     1561 cgtaaaattt ttgactgcca aaagaatttc agccagttga ttcatttttt cgatatctgg
     1621 acattgtgat cgaaaccagc gataatttct tgtaaatggg ttttttcgca gggaaatgta
     1681 atatcggtaa gttctccaaa gcgcatcata tcgggcatgc gcttcaggtt ttacaggcag
     1741 gatttctgaa atggcgatat cgaggggtag aatgtggttg agtcgatagg aaagaattat
     1801 cagctgttgt tttggcatag gatcgaaatc gaaatgggca taaaaagagg aagcatgcac
     1861 ccctgtatcg gtgcgcccac aacctgtaat gtcaataggt ttctttaaaa gaatgctaat
     1921 tttttcttct atctcctgct ggatgctggt agcatttttc tggcgttgcc atccacaata
     1981 attggtgcca tcaaaggcaa gatgaataaa atatctttgg ttgtccaccc ttaatcgaac
     2041 accatctttt gaacttcaac gcctggaatt tgcttgagcg cttcttctaa tttcagcatc
     2101 tctcctacgt cgccaaccag ttccaaaatg ataaggcctt cctggctgca ataatcttcg
     2161 cttgcttcgt gtaatcccag tcgcgtatga atagaacaac caaatttggt caaaacatcc
     2221 tgtacttcgg ttgatttttc ggctctctgc gtaattttaa gtcctaaaat cgtcgttttc
     2281 ataagaatat tttttggttt ttggcaaatt taattccttt ttcttaattt ttacatcatt
     2341 taccgttaat ttcgaaccat ttcttgagtt gttcgaaatt atccaccaca acagcttttg
     2401 tttcgataag acgttgtttc gactgatgac cctgagctat tagcacaacc ttcatcccca
     2461 aacgatctcc aacttcaaaa tcatggatag tgtcgccaaa caggtaagct tcgttggggt
     2521 tcattttgtt gtcggtgata aaatgtctgc cttcttccat ttttcctccg ccatagtgat
     2581 tattaatacc tgagatagct tcgaaataag gatgaatacc tttgtcgagc aatattttat
     2641 cgagcgattg ttgttccatg gccgaaagaa tgtactgccg gtaaccttta ttgtcgaacc
     2701 attcgagtat ttcaacagca ccatccatca gcggaacttt ttctatatgt tctaaaaacc
     2761 tcttcataaa ttcaagacca atcacctcga agggatccac attgaagttg aatcctaatt
     2821 tcttataata ttctattacg gggaaatcaa aaatctccct gtatctttca tgatttagca
     2881 gaggaagatg tcggtcattt agcaatgaat tcattacatc gatgcataat gaaacatcat
     2941 cgagcaatgt gccattccaa tcccagataa tgcccgtaga tttgttgtcc atcgaggtat
     3001 ctttaactga ttacaacaat taggttttta tttttgcaat agaagacgtt ttatttggcc
     3061 ggttcaacct gaaccttcgt cttttgattt aagagggttc ttttgaaaaa tcttcagcca
     3121 ccccaagaat tcgggaaata aggcagctga agatagtaaa tgcctcaaaa attgagagta
     3181 gagtttagtg gtgtttttta gcatgttata gaaagaactc cggtttatcg gagttttttc
     3241 cattcttctt tcattctgtg aatgtcgtta atccagtcct gaatgtcgtg ttcgtgttcg
     3301 agttcttcgt tgaggatttg aacggccatc tggtaggttg catgatcctt accactggta
     3361 aagttggcta tttgttcgta acgctcgata gcgcaccttt ctcctttaag gttttgatcg
     3421 agtataacct ctatgtaagg gtctttgggt tcttcgtatt ggcagtgagc taacttgctc
     3481 cagtcgttgg gatttagaat gggtgttcct tccagctgga taattctgtt aaccaataat
     3541 acggcgtgat ttaattcctg tgtagcatgc agcagcagtt caggttcaat ttcggccctc
     3601 atcggccctt ccatcaacct tgccccaatc cagtattgat aataggcaag ccattcttcc
     3661 gctaaggcgg cattcaacat cttcaggagc tcatcccggt cgactgataa aatcttaata
     3721 gcttcttttc ccatgatcca tctgtttttt agttgttaac tatttgatat ggtaaaacca
     3781 gagtttcctt tatatcgcct cgtttcgatc caataaattc tgattacaaa tttataaaaa
     3841 tttttctatt gatgaaacag gctgaaaaaa tttatttacg aataaggccc tttaaaacgt
     3901 atatcgaaat attttcattg taatattctc gataggctct cataaggttt tacgaattga
     3961 taaaggagtt gccatttaag caattgataa aacgatttcg gtgaaataag agggagaggg
     4021 acaaatataa gtttcatcgg gagtgatccg ggggggattc gaaccctcga cccgcagctt
     4081 agaaggctgc tgctctatcc aactgagcta ccggaccatt ttgggctgca aaattaagtg
     4141 tttttttaga aatttcgaat cggatgaaga ccgcatgaaa atgaatccca tccttttgtt
     4201 caggctatta ctgcattatg ctaagttaac tggtattata taagggggta cacattcctt
     4261 ctctgttaag ataaactttt gattaattcg acggagtgga tctgtatttt ccctacgaat
     4321 ctcgcatatt ttctattctt tcttcctgcc aatattttgt atctgacggc tcgaaaggct
     4381 gaagttttgg ctggcattac gggtttattc attgaaaata tttaactgtt gcttcgcttt
     4441 ctgccttcag tcggctcaat gacttgattc ctcaagattc atgaacgttc catgccaaaa
     4501 atatatttat tcgggatggg aagaaggatg tttgcgaggg ccagcaattt gccactcttc
     4561 gaatgagtcg agaattatac atggcgcggt attcaatcca ttagtcagga aaatcggtca
     4621 ttgtcaggcc atgggtcgaa atatcaaagg ggatatggaa gtcgaatatt gacatagttt
     4681 gccaatagaa taactatatc atgcttatct ttgctatgat ttaccctaat ttttacctat
     4741 atggattcca ggcgttgctt ttatttttta gagatattta tcttttcatt ttttcggctt
     4801 caagcccaaa ttattcacac tgaacctgtt ttccccctcg tgaatcaatc tgtcataatt
     4861 tattacgacg ccgctgaggg aaatcaaggt ttgatgggat atacaggaga tgtttatgct
     4921 catacgggag tgattaccaa cctgagcact tctccttccg actggaaata tgtgaagacc
     4981 aattggggac agaataccca agaaaccaag ctcgagagaa tttcggccaa ttattataga
     5041 cttacaattt ctcctgacat caggtcttat tatggtgttc cggcaaatga acaaattctg
     5101 aaaatggctt ttgtcttcag aagtggcgtc acaatagggg gttcttataa ggaaggaaaa
     5161 gatgtaggaa atgccgatat ttttgtggat gtctatccgc ctgacttatc cattaacatt
     5221 acttctccac aggggaatca tttaatcgaa gcggaaggag ataccatttt ggttcatgcc
     5281 atcagccctc aggctgattc aatgtgggta tttgaaaatg gcagtcttcg aaaagccgta
     5341 gcaggagttt ttatggatga tacccttgtg gcgagtccaa taaatggata ttggaacgat
     5401 catcatctta tttttgtagc taaaaattct cagcagatag taagagatac cttgttttat
     5461 gttgtaattc cgcaacccac agtggtggag ttacccgatg gaatatacga cggcataaat
     5521 tacgttgacg attcaacggt tatattatgt ttgcaggctc cggggaagaa aaatgtattc
     5581 gcaattggcg atttcagcaa ttgggagatt ttgccagaat attatatgaa taaaactgca
     5641 gatggagaaa gattctggtt agagataagc agtttgattc ccgccaaaga atatatctat
     5701 caatatttag tggatggaaa aatcaggata tccgatcctt attgtgataa aatcagtgat
     5761 ccgtggaacg ataaatatat ccctacgtcg gtttatcccg caatgctaat ttacccaagt
     5821 ggaaaaacta caggcatagc gtcggttctt caaactgctc aagaaccatt tgcatggcaa
     5881 atcgaacaat tttcgcctcc atcgagggag aaactcgtta tttatgaatt gcttatccgc
     5941 gattttacaa caaaacatac ttatcaatcg gtcatcgaca ctttaaatta ccttgtcaaa
     6001 ctgggagtca atgccatcga gctgatgcca ttgatggaat tcgaggggaa ttcgagctgg
     6061 ggttataatc ccatgactta ttttgcccct gacaaatact atggccccaa gaacgattta
     6121 aaaaaattga tagatgaatg tcataaaaga ggaattgctg ttattttgga catagtgctg
     6181 aatcatgcat acgggaataa tcctctagta aaactatact gggatgatgt aaaacagatt
     6241 ccggctgctg ataatccatg gttcaatcag acttcgccca atccaagcta tagctggggt
     6301 tacgatttca accacgaaag tccttataca aaacaatttg tatatcgagt aacacgctac
     6361 tggcttacag aatataaggc agatggtttt cgtttcgact ttagcaaagg atttaccaat
     6421 aagcctgggg atggctgggc ttatgatgcc actcggattg ccatactgaa aaattatacc
     6481 gattctattt ggtcagtcaa tcctcaggct tatgttattt tagaacattt taccgataat
     6541 ttggaagaaa aaattctggc caactatggg atgctgcttt ggggaaacct taacggagcc
     6601 tattgtgatg catctatggg atatacaagc agtagcgatt tttcatcgat cgattaccag
     6661 cgccgcggat ggaataatcc tcatgtagtt ggatatatgg aaagtcatga tgaggagcgg
     6721 ttgatgtaca aaaacctgac ttggggcgtt tcaaccaatc cagattatga catcaaagat
     6781 atgactattg ccctcgaaag agctgctctg gcggctactt tctttatccc tgtacccggg
     6841 ccaaaaatga tatggcagtt tggcgaactg ggttacgatt acagcatcga ctataacgga
     6901 agggtaggag agaagccggt acgctgggat tactataccg actggcgccg ccactatctt
     6961 tatgaggttt actcggcact ccattatctg aaaaataatt atccagtttt taatacaacg
     7021 gattacagtt ataaactcaa tggggtcgat cgtagaatca tactccgaca ttcggatatg
     7081 gacgtagtga taacgggtaa tttcgatgtg aatgccaaag atatcgttcc tggattcacg
     7141 cgtgatggaa aatggtacga atttttcagc ggcgattcac tcgaggtgca aaacttgacc
     7201 gcaaccatta ctttcgagcc cggagaatat catttatgga gcacctcacg cattccaaaa
     7261 ccagccagct tgaatacggg ttttgtcgaa atggaagaac aaccctcttt tcgtatctgg
     7321 ccaaatccgg caaatgagaa aatctttgtt tctttctatc tcgagcaaag aagccaggtt
     7381 gagcttgtgc tttacaatat ttcgggtcaa aagataggac aaagaatttt aggcaggata
     7441 gaggctggtt cttacacaaa agaggttcca ttgtccgata ttgctcttca ggctttggag
     7501 actggtatat acatagtgga attgaaaact aacaacagga tacaatattc aaaattagcc
     7561 ataggtgttt attgaagaac tgtttttaaa agtattttat ggatggattt attaaactca
     7621 cgggagtttc tatattgaat catttgcaaa agaccttttt atcccggatt tattatcgag
     7681 gtataaattc taatgaaggt cattagaaaa atgtgataca attatttgta ttcctcacaa
     7741 tatcccaaaa gaaaagatgg gtcatgcggt tttggaaccc acaatttcga cattcatgat
     7801 ttatgagcta atgaaagcgg gaattttgtg atggtgccct atatcctgat gagttcgttc
     7861 aaaaagccaa attaggaaaa gaatgagggc cattcttgaa tgccattcga taaaaaacgt
     7921 ttgtaagtag aggcatcttc ataaaagaag atgatactct cgaaattatt cttagtcatg
     7981 gaacgacggt ggtttattgg actctgtaaa aacaagtttt gagtttggtt agcgtcatgg
     8041 ttcaggtttc cgatggcaat tcggggttta tcttttttct taccagccgc cattctcttt
     8101 tctgtaaaaa ttttgtatca attccttata tttttccatc accactcttc ttttcagttt
     8161 cagtgaagga gataattcac cggtttctat cgtccaatcg tcggctacga gggcaaaacg
     8221 ttgtatactt tcattctcct ttattccagg tccattcaat attttaattt cttcctcgat
     8281 taaagatata actttctgat tctgtataat ttcaagatta gaattgaaat agataccttg
     8341 atgggcaaag tattccttta tataatcaaa ctctggaaga ataattgccg aaagaaaact
     8401 ttgatgttca ccaacaacaa aggcttgact gataaaaaaa gattgttgaa gtttattttc
     8461 aagtttttcg ggatttacat aaattcctga agccatttta aaaatttcat tttttcgacc
     8521 cgtaatagtg ataaaacctt cttcgtcaat tgtggctata tcgccagtat gaagccatcc
     8581 atctggttcg attacttcgt tggtaagctc ggggtgttta taataaccct tcatcacatt
     8641 tggtccgttc accattaatt ctccttcatc cgatatcttg acgagaatat cgggaatagc
     8701 cttgccaatg gttcctacct taatggcaga ttcagtattg aaagcaacca gaggagaggc
     8761 ctcggtaaga ccataacctt cgtaaacttt gataccagca gcccaaaaaa tattcaccaa
     8821 ttcaggccga atacttgctc cacccgttac aatcttcacc aatgaacttc ccaaagcttc
     8881 acgccacttc acaaaaacca gtcgatcagc aatgcttaac ttccaacgat accattttcc
     8941 caaatcctta ttgatttcaa acctttgtgc aaggctcaag gcccaatcga atacaaatcg
     9001 ctgaattctg ccaagctcaa gccttttctt caatatcccc tgataaacct tttctaacaa
     9061 caatggaacg gtaatcatca attgaggctt gatttcctga aaattgagga gaatattcgc
     9121 cgtaacatcg gcataataaa caggcatacc caaataaatt gttgcataat tcaccatacg
     9181 ttcataagag tgagataaag gaagaaaact cagttcagta tatgtagatt ttaaattaat
     9241 agtaaaagcc gccactaatg agttgcacaa atgattttca tgcgttagca tcacgccttt
     9301 gggcaagcct gtcgttcccg atgtataaat gatagaagcc agatcgtatg gcgatacagt
     9361 ggctttcaat cgatcgagca aatctttctg aatattggat ttcgcgaaat ggataaactc
     9421 tgagaagggt atacagcctt ccacttcatc gaatgaaata attgctatca aggaagggag
     9481 ctgttccgaa aaatccctga ttttaccata taaatatttg tttccaacga aaatgagttt
     9541 tacttcggct tctttcaaga tatacatcaa gtcatcatcg ctgatagtag gataaatagg
     9601 gacatgaaca ccaccaatct gaagtatccc catatccaca aaattccatt caggcctgtt
     9661 ggtgcttatt gtacttattt tatcctcttt tcttacaccc attgccatca aaccgtaact
     9721 tacccaattg gcgtattggg cgtattcttc tgaagaatac gtaatccatt tacccgcttt
     9781 cttacctgcc aaagcaatgg gttgatggta acgttcaacg ttgtattcca ataaatcgaa
     9841 aattcttcta catctcattt tattaatagg tatcgaatga aacttgcaaa ataaataaat
     9901 ttaaaactga aaacttgccg aggatgtctt tggttattaa atagcaagcg cttaggctaa
     9961 tttcaatctt acagaaggaa gaagaacttg tgatgtaact atgtcctgag aaaatagatg
    10021 taaatacata attcgcagtc aaatttttat aatcgccttt gccaaaattt tttccattat
    10081 taattcgtcg gcattttccc taacaataaa gaaggaaaca agaaaggaga agcaacttca
    10141 tgaaataatc agatagccga atacaactcg aatgctcgtt gtgcgaaatg tcggatagca
    10201 gccgaggtaa cagggaaagc ctcgatatat ccgttatggc cgacattttc gagtacgagg
    10261 atctcagaat gtgagggaag aaaaatttgg gaaaaataat ctttggccga tatgcggctg
    10321 tcctgcttac caataatgaa aagaacaggt gcctggatat gagtaagaac gtccaaataa
    10381 gaaggtcttt ccttcattcc tatttgagcg gcaataatag cttgcggctt catgtacgag
    10441 gccgactcga tgagcatttg gatttccatt tgatattttt ccgcattact cggagcaaat
    10501 aatgatggaa taaattgcaa aataaatccc gttttgtttt gttgaatgaa ttcaattgtc
    10561 cgttcacgat ttttgcggcc ttcatcagaa tcgggtaaag cagaagaatg aaacaaaaca
    10621 agccctttta cccgctcagg gaatttatca gcaaaggcaa gcgaaacata acctcccata
    10681 gaatgcccga ccaggatgca atctttcacc cccagatggt ccaatatact cttagtgcaa
    10741 tcagccatta gttccatacc atggacagga gcaatgatat cgctttggtc atgtcccggc
    10801 aaatcgaagg taattgattg aaattcattg gagaatgaat tagcaaaaga ttcccagatt
    10861 ttatgagatt ccatgaagcc atggagaaaa acaatagccg gccctagacc tcggctttca
    10921 taataaacgg gatgattttg aaatgacatg aaaggcatag aaacgagagt ttgaacaaat
    10981 gtgcgaattt aggagatttt tgtctaataa gacaaggtga gccaaatgaa cgaagtagtt
    11041 ttacatctcc aatttttcat gaaggtataa atttcgatga cgaatctacc tttattggtt
    11101 tttggatagt gccttaaggc ctattgagcc aaccgggcta tcataagttt tgagtggata
    11161 tttgggattg atgtatcgat gctaaaaaga ggcccttagg catgatacct gagggcctcc
    11221 taataattag tcatgaagag gaaagattat tctttagcaa gggtttttcg ggcagaatag
    11281 ttgatcttga atgctcccac ctgcctaagt tcctgtttga tatctgttac gattcccata
    11341 cgggtttctt cgtccacttt taatgaagtt gtaagtagag gtctatccac ttcatcacgg
    11401 gcttctcttt cgcgagtaat aaattcacga atatcggcca ccgaagcaaa ctgattattg
    11461 agctgaatgc ggctttctgt accgtattta taatcaatgg gttgtcctac ataaatgaaa
    11521 ctaaccaaag attttctttc gagctttgcg acttcggtag cctgaggaac ttttactctt
    11581 acaattaaag aactttctct aatggtagta ataaccatga aaaaaaacag gaacataaaa
    11641 ataatatccg acattgaacc tgtattgatt cctggtgttg aacgtccttc cgatttttta
    11701 aatcttgcca taattattta cctcctacgt tttcgggttc ggcttctgaa atcgacattg
    11761 gaatagcttt ttgtatagca tcgatatatt gctgatttga taattggcta aatttcaccc
    11821 caaagcgttc cattgaaagc tcgtcgcgca gctcattaaa agctgcagtt agctcgttct
    11881 gaactttaat gtacatttta taggatgtac ctcggtcatt ttttagtgat ataacgcctt
    11941 tcgatgtttc gacttctcct aaaccaggaa ttatctccat tttcttttca gggagatctg
    12001 gacgattata gggatttgca aggaactcct tagcagcggc tctaagcata ctaatatcca
    12061 taggtcggcc ttgtacaagc agttcgtcat ttttatttac caaaacagta aatatattcc
    12121 tctttttaat atcaacctgt tcttgagtag gatccggtgg agggggcaaa cgtcttagaa
    12181 tacctaaatc cgtgtcaatt gaagaagtta acaggaaaaa ggttaacaag aggaaggaga
    12241 tgtctgccat tgaaccggta ttaagttctg gccttgcacg tttcatgttg attccatttt
    12301 taaggtttat ttcttaaacc attttacaac ttcaaaataa agaatagaaa gaattaatcc
    12361 tccgaaaagg atataagtag caataattgt acctccgatt gcctttgaaa gagtaggacc
    12421 aatggaaaat ttatcataga aaggtccctg atcggctggg gatagtaaga ttgctattac
    12481 aataagagca atgattccca aaaaacctaa tagccccccc ttagcctgct taaaatcctt
    12541 gaatacagac attaaaggaa aaacaataac agacacaagg gcaactaagg ctaatgcata
    12601 cgttatataa atgccaatat ctattaatgt agatgacatg ggtttttatt ttttagtgtt
    12661 tttatatcta accaaaatat ccataaacga tatagttgaa tcttccatat cgttaacaat
    12721 ggattctacc ttacttagga gataattata aaatacctgc aaaataagag caacaattaa
    12781 accgaaaacg gtagt
//
LOCUS       DAOORU010000054        12521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_6233_length_12521_cov_4.650168, whole genome shotgun sequence.
ACCESSION   DAOORU010000054 DAOORU010000000
VERSION     DAOORU010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12521
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6233_length_12521_cov_4.650168"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            323..808
                     /locus_tag="PLF20_04965"
     CDS             323..808
                     /locus_tag="PLF20_04965"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HOX80388.1"
                     /translation="MISIDSVKLIETMLAPGIMISACGLLLLGIHNKYSIVVNRIRVL
                     EEEKRSLIPGFVEKTLNIYQSKRLESIQSQIVRFEYRVKLIRNVVFFYTLSVALFVLS
                     SLSIGLSYIIESRWAESFSMAFFLAGMISVLIGIIFAAREVWKGYEIVQIEVQESRIP
                     E"
     gene            817..2157
                     /locus_tag="PLF20_04970"
     CDS             817..2157
                     /locus_tag="PLF20_04970"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOX80389.1"
                     /translation="MEQQDTIIHNGLLLTMDREARILPHHSIRIIDGIIQEILPVESS
                     KDDHGIRVINASGSLVMPGFINCHTHAAMTLLRGFADDKPLHEWLSDYIWPAEAVFMN
                     RENIALGTRLAILEMIASGTVMFSDMYFFEDEVARACEEAGMRVLLGEGLLGNHTPHF
                     TTPYQGIDFTETLAEQYKNHSLIEVAYAPHAVYTCPREILSEVALRSEKNHLPVHIHL
                     AETAEEVENCIASTGKHPVDYLDEIGLLDQRLIAAHVVHVEEEHLKLLTHKGVRIAHN
                     PSSNLKLNSGFPPIHKQIQYKIPLGLGTDGAGSNNNLSIPVEMRHTALVDRLLHQNRE
                     SDQAFQIVKMATIGGAKVLGADQITGSLEAGKAADLIIIPLDKPHLQPIYNIYSTLVF
                     SLHSSDIDTVMINGKIVMKDRKILTLDEESLLKDLKRVAGDIREKFKMKNHLAY"
     gene            2163..3458
                     /gene="rimO"
                     /locus_tag="PLF20_04975"
     CDS             2163..3458
                     /gene="rimO"
                     /locus_tag="PLF20_04975"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HOX80390.1"
                     /translation="MESKGIKIYTLGCYKNQVDSEKLAGKIESAGINAMHTDKFEGNT
                     LIINTCGFIKDAKEESIEVIMQAIEAKKRGRIGRIVVMGCLSERYKKELREEIPEVDA
                     WFGVESIEQIIQWLNLPEYRERRIIRKLANPPHYAYLKIAEGCSRRCSFCAIPNIRGS
                     HRSRPIDEIMEEAEGLASKGVKEIILISQDLTSYGIDLYKKQMITQLVKHLSESNLFP
                     WIRLHYLFPTSFPEDLLHLMATKDNICPYIDIPLQHISERILKSMHRGIDKASTMALI
                     EKFRTLVPHAALRSTFIVGYPGETEKEFDELLNFINEVRFERLGVFTYSEEEGTPAAL
                     LKDDVNEFTKKRRAELLMEIQSEISFEKNESLIGKTMKVLIDRKEEKYFIGRTIYDSP
                     EVDNEVIIDATHTSLQVGQFYPIIIEKADLYETFGKPYK"
     gene            3474..4208
                     /locus_tag="PLF20_04980"
     CDS             3474..4208
                     /locus_tag="PLF20_04980"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HOX80391.1"
                     /translation="MKHLRTFIFISVICLSMKPVFAQTYDSIKNTRDDKGSKQGYWEE
                     NINRQLEKGWYVDNKREGVWRTYYPKGFLHEVIEYKDGKRGGLRLEIDSRGYLAVEEY
                     FENDQLHGRSINYSMGAHPRVEQWYKNGQPDGLKKIYYDNGKVQEESNYKNGKRHGIS
                     RWYNPKGQVIAEFNYFEGEFDGVQKTYNDAGILIKSEEYSKGVLHGNYSEWDDAGNLI
                     ISGQYKNGEKVGKWKEYKGEKEEEKE"
     gene            complement(4536..5783)
                     /locus_tag="PLF20_04985"
     CDS             complement(4536..5783)
                     /locus_tag="PLF20_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX80392.1"
                     /translation="MRDIFEKIATNKGPLGQYGEDAFGYYMFPKLEGEIGHRMIFGGR
                     ERIIWSLNNYLALANHPEVRKVDAEAAARWGLAYPMGARMMSGQTIYHEQLERELAEF
                     VRKEDAFVLNYGYQGMISVIDSLVDRKDVIVYDSDAHACIIDGVRLHLGKRFVFPHNH
                     MENLEKQLQRAAHLAEENRGGILVITEGVYGMSGDLGKLCEIIELKKKYPFRLLVDDA
                     HGFGTMGETGAGTGQYLGVQEGIDIYFGTFAKAMAGIGGFVAGSKLVTDYLRFNMRSQ
                     TYAKSLPLAMVIGALKRLEIIRTHPELREKLWLIANELQKGLLNAGFNIGNTQSPVTP
                     VILSGSVSEATGLIYDMRESFDIFCSIVTYPVIPRGQILVRLIPTADHTLEDVSYTIE
                     AFSKLKDKLERGEYGHDNTVRSF"
     gene            6049..6852
                     /gene="bamD"
                     /locus_tag="PLF20_04990"
     CDS             6049..6852
                     /gene="bamD"
                     /locus_tag="PLF20_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOX80393.1"
                     /translation="MQKNAYLISFILLTVIILTGCSKYQKIYKSTDNDLKYEAAIAFY
                     EKKDYYRALQLFDQIIPAFRGTEREEKISYYYAYAYYYQKDYILAGYYFKRFADTYPT
                     SRYNEECLFMTAYCSYLNSPPYYLDQTSTVDAIRDFQVFINRYPTSSRVPRANELIDQ
                     LRLKLEEKYYRISLLYLQMEEYKAAIVSFQNLMKDYPDTKFREEAMYYIVEAWYKYAE
                     KSIPEKRIERHQAAIDAFNDFKAQYPESRYLQNAQNLIKNSFKIAKIKI"
     gene            6877..7209
                     /locus_tag="PLF20_04995"
     CDS             6877..7209
                     /locus_tag="PLF20_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008822709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOX80394.1"
                     /translation="MLHKYKRLHADTTAVTRRINDFDAQTGNIYETVAILAKRANQIS
                     NELKEALDAELKAFQTTSETLDEVYENKDQIEVSCKYEQMPKPTLLAIAEYLNGKLYW
                     RNPVKEIQ"
     gene            7219..8421
                     /gene="coaBC"
                     /locus_tag="PLF20_05000"
     CDS             7219..8421
                     /gene="coaBC"
                     /locus_tag="PLF20_05000"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOX80395.1"
                     /translation="MLEGKNILIGITGSIAAYKIPFLIRLLIKKGACVQAILTPTACN
                     FVTPLTISTLSRRRAIVDMFEKESGEWNSHVELGNWADLMLIAPTSANTISKMASGQA
                     DNLLLCTFMAARCPVFFAPAMDVDMYQHPSTQANIRKLQEIGLHLIAPQTGELASGLT
                     GTGRLEEPEVIISVLDDFFKKKTTLKDRKVLITAGPTREPIDPVRYISNHSSGLMGFA
                     LAEKAAEMGAKVTLISGPVKLETTHPEIRRINVETAQQMAETTLHYFSESNIIIMAAA
                     ISDFSPVEPSPEKIKKHEQETLVLNLKPTMDILAEMGRRKKNDQILVGFALETNNEKD
                     NALFKLRNKNADIIVLNSTRDKGATFGFATNRVTIFHKNGDRAQSELLPKTEIAQMIL
                     DFIIQHHF"
     gene            8436..9332
                     /locus_tag="PLF20_05005"
     CDS             8436..9332
                     /locus_tag="PLF20_05005"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HOX80396.1"
                     /translation="MRRFGIFIGILLLSIASIGQELDCQISVSTRQVEGTDRQVYNTL
                     QTALFEFMNNKRWTNYQFKLEERIECTMLIQISERLSVDEFKGTMNVVLRRPVYKSSY
                     NSILLNHVDKDIQFNYVEFEPLDFQESAITSNLTAILAYYAYILIGLDFDSFSLYGGT
                     PFYEKAQTIVNMAQNLPQKGWKSFESMRNRYWLAENFNNTSYNKIRQFIYQYHRQGLD
                     LMAENVDQGRTNIIESLELMRQAYRDKPNSFLLQLILDAKRDEFINILTGAPDQDKVK
                     AVNLLKEIDPANSTKYQTVLTK"
     gene            9394..11046
                     /gene="recN"
                     /locus_tag="PLF20_05010"
     CDS             9394..11046
                     /gene="recN"
                     /locus_tag="PLF20_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOX80397.1"
                     /translation="MLCRLSVKNYALIEELEFEPGPGFNIITGETGAGKSILLGALGL
                     ILGNRADMQALRDNSQKCIVEGTFKVNLEPVNRFLYENNLDVCDELIMRREILPGGKS
                     RAFVNDTPVNLNALKIIGDMLVDIHSQHENLLVNEAQFQLAVLDDFARNKEILSSYYA
                     QYLDYRNKTTKLELLSEQEAKSMAEQDFIHFQLDELHEAHIEPGEQAKIENELSLLTH
                     AEEIKTRLSEMIQILDDEDSGVIPALASQFSNLNSLIRFDEALKEIESRYTSCFYELK
                     DILQELIKYADTIHYTPDRIAALSQRLDILFRLQQKHRVKDADELIEVKEHFIEKLNS
                     YASLNEQIESLQQEIIWIEGELEEKANRLSDRRKAAIQPIQHQLKESLSELGMPDASI
                     EVVLESTTDFIETGKNRVNFLFSANVGQEPRSISKVASGGEISRLMLAIKSLISNKTM
                     IPVLIFDEIDAGVSGGIAAKAGRLMKQMARNMQIIAITHIPQIAALGDNHFLVSKKIN
                     KGTTQSIIERLSTEGRIRELAGMLSNHEITQEAIAAAKQLLK"
     gene            11086..11916
                     /locus_tag="PLF20_05015"
     CDS             11086..11916
                     /locus_tag="PLF20_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX80398.1"
                     /translation="MSYNLLKGKKGIIFGALDHKSIAWKVAEKAYEEGARIVLTNTPA
                     AIRFGEIEQLAEKTQSIVIPADATNVNDIENVYLQGMEFLDGKIDFLLHSIGMSMNVR
                     KNRPYDDVDYDYFLKTLDISALSFHKIVQVAKKLDAIEEWGSIVALSYIAAQRTLFRY
                     NDMADAKATLESIARSFGYIYGREKKVRINTVSQSPTLTTAGSGVKGIDDLYDFTDRM
                     SPLGNASAEECAAYIITLFSDLTRKVTMQNLFHDGGFSSMGMSARAMTMYCHSLNPEE
                     "
     gene            complement(11989..12174)
                     /locus_tag="PLF20_05020"
     CDS             complement(11989..12174)
                     /locus_tag="PLF20_05020"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOX80399.1"
                     /translation="MKTLKEVEIEHILEALRLTNGKVSGEDGAARLLGINPKTLDSKI
                     RKLGIKKVLDIQHPGTS"
     gene            complement(12171..>12521)
                     /locus_tag="PLF20_05025"
     CDS             complement(12171..>12521)
                     /locus_tag="PLF20_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80400.1"
                     /translation="NRKLEEEIKAGRFRSDLYFRLNAFPIYIPPLRERVEDIPALIHY
                     FIEYHGGVLGKPYLKISEMDMQKLMNYQWPGNVRELKHIIQKAVILTTGDILILVISK
                     HRPLCFKSKNSEKQ"
BASE COUNT         3980 a         2654 c         2476 g         3411 t
ORIGIN      
        1 ttctctttct ctttctcttt ctctttctct ttctctttct ctttctcttt ctctatcccc
       61 ttccctatcc ctatctcact ttttcctatt ttttcttttt atctaaaatt caaggataga
      121 atgaattctt tttattatat tttattgtaa tccaaattat tacatcatat tattctattg
      181 accgttgtca gaatctccac actaataata aatttgagtt aaaccttgtg atagaaggaa
      241 atttcgaaag gataaagatg actctggttg gttgttatgt tctgactaat tttgcaccat
      301 aatcaataaa tctcaagaaa gaatgatatc aattgattct gttaagctta tcgaaaccat
      361 gctggctccg ggtattatga tttcggcctg tggactactt ttattaggca ttcacaataa
      421 atacagcatt gtagtaaatc gcatccgggt actcgaagaa gaaaagcgaa gcctcattcc
      481 tggttttgtg gaaaaaaccc tgaatatata ccaaagcaag cgtttagaat ccattcagtc
      541 gcaaattgtt cgtttcgaat acagagtgaa actcattcgc aatgtagtgt ttttttacac
      601 cttgtcagta gccttgtttg tactgagttc attgagcatt gggttgagct atattatcga
      661 gagcagatgg gctgaatcct tttctatggc tttcttcctg gcaggtatga ttagcgtatt
      721 gataggaatt atttttgctg cccgtgaagt atggaaggga tatgaaattg ttcagataga
      781 agtgcaggaa agtagaattc cggaataaac ccaaaaatgg aacaacaaga tacaatcata
      841 cacaatgggc ttcttttgac aatggataga gaagctcgga ttttgcccca tcacagcatt
      901 agaattatcg acggaattat ccaagaaatt cttccagtcg aaagttcgaa agatgatcat
      961 ggtattcgag taatcaatgc ttcgggcagt ttggtaatgc ctggattcat caattgtcat
     1021 actcatgcag ccatgactct tttgcgtggt tttgccgacg acaagccttt gcatgaatgg
     1081 ttgagcgatt atatctggcc agccgaagcc gtattcatga atcgtgaaaa tattgctctg
     1141 ggaacaaggc tggcaatcct cgaaatgata gcctctggaa cggtaatgtt ttccgatatg
     1201 tatttcttcg aggacgaagt agccagagct tgtgaagaag caggtatgcg tgttttatta
     1261 ggtgaaggat tattgggtaa ccacaccccg cattttacca ctccttatca gggaatcgat
     1321 tttactgaaa cactggccga acaatataag aatcattcac tcatcgaggt agcctatgca
     1381 cctcacgctg tttacacctg tccaagagaa attctgagcg aagtcgctct gcgttcagaa
     1441 aagaatcatt taccggtaca tattcatctg gccgaaaccg ccgaagaagt cgaaaactgc
     1501 attgcatcga cggggaaaca tcctgtggat tatctcgatg aaataggttt gctcgatcaa
     1561 aggttaatcg ctgcacatgt tgtccacgtc gaagaagaac atttgaaact tctgactcac
     1621 aagggagttc gtattgcaca caatccctcg agcaacctga aactaaactc aggcttccct
     1681 cctattcaca aacaaataca atataaaatt cctttaggac tgggcaccga tggagctggt
     1741 tcgaacaata atcttagcat cccggtcgaa atgcgtcata ctgcgctggt agaccggttg
     1801 ctccatcaaa accgcgaatc agaccaggca ttccaaatcg ttaaaatggc tactattgga
     1861 ggagccaaag ttttaggagc cgaccagatt acaggatcgt tagaagctgg caaggcagcc
     1921 gatttgatta tcataccact cgataaaccc catcttcagc ccatctacaa catttattct
     1981 acacttgttt ttagcttgca ttcatccgat atcgataccg tgatgatcaa cggtaaaatt
     2041 gtgatgaaag atcgaaaaat tctcacgctc gacgaggaat cactgttaaa agacttaaag
     2101 agggtagcag gagacatcag ggaaaaattc aaaatgaaga accacctcgc atattgaaat
     2161 aaatggaatc gaagggtata aagatttata ctttaggatg ttataagaat caggtagata
     2221 gtgaaaaact tgctggcaag atagaatcgg caggaataaa tgcaatgcat actgataagt
     2281 tcgaagggaa tacattgatt atcaatacat gtggatttat taaagatgca aaggaggaat
     2341 ccatagaggt catcatgcag gccatagagg ctaaaaagag aggcaggatt gggcgaatcg
     2401 tcgttatggg ttgcctgtcg gagcgctaca aaaaggagct acgggaagaa attcccgaag
     2461 tggatgcatg gtttggtgtg gaatccattg aacagatcat acaatggctc aatttgcctg
     2521 aatatcgaga aagaagaatt attcgcaaat tagccaatcc cccgcactat gcctatctga
     2581 aaatagcgga aggttgcagc cggagatgtt cattttgtgc cattccaaat attcgtggct
     2641 cacatcgttc gcgccccatc gacgaaatca tggaagaagc cgaagggctc gcctcaaagg
     2701 gagttaaaga aattatcctg atctctcaag atctcacttc gtatggaatc gatctatata
     2761 aaaagcagat gattactcaa ctggtgaagc atctgagcga aagcaatctc ttcccgtgga
     2821 tcaggcttca ttatcttttc ccgacctctt ttccagaaga cctcttgcat ttaatggcaa
     2881 caaaagacaa catctgtcct tatatcgata tccccctgca acatatttca gaaaggatcc
     2941 tgaaatccat gcatagagga attgataagg cttctactat ggctctgatc gaaaaattca
     3001 gaactctcgt gcctcatgcc gcacttcgtt cgacattcat cgttggatat cctggcgaaa
     3061 ccgaaaaaga attcgacgaa cttttaaact ttattaatga agtcagattc gaacgcctgg
     3121 gtgttttcac ctattcggaa gaggaaggaa caccggcagc cctcctgaaa gatgatgtga
     3181 acgaatttac taaaaaacgg cgagcagaat tactcatgga aattcaatcg gaaatcagtt
     3241 tcgaaaaaaa tgaatcactt atcggaaaaa ccatgaaggt gcttattgat agaaaagaag
     3301 aaaaatattt cataggacgt acaatttatg attctcccga ggtggataat gaagtgataa
     3361 tagatgctac gcatacatca ctacaagtcg gccaattcta tccgataatc atcgaaaaag
     3421 ccgatcttta cgaaactttt ggaaaaccct ataaatgatt aaaccattgc aatatgaaac
     3481 acttaagaac ttttattttc atatcagtaa tttgtctgag catgaaaccc gtttttgccc
     3541 aaacctacga ctccataaaa aacactcgag acgataaagg ctcaaagcaa gggtattggg
     3601 aagaaaacat caatcgccaa ctggagaaag gatggtacgt cgacaacaaa agagagggcg
     3661 tttggagaac ttattatcca aagggatttt tacacgaagt gatcgaatat aaagatggga
     3721 agcgcggtgg tttgaggctc gaaatagata gccgtggata tctggcagtg gaagaatact
     3781 tcgaaaatga ccagcttcat ggccgttcta tcaattactc catgggagct catcctcggg
     3841 tagagcaatg gtataagaat ggacagcccg atggcttgaa aaaaatttac tacgacaacg
     3901 gcaaagtgca ggaagaatcg aattacaaaa atgggaaacg tcatggcata tcacgctggt
     3961 acaatcccaa ggggcaggtg atagctgaat tcaactactt tgaaggtgaa ttcgatggtg
     4021 tgcaaaaaac ttacaacgat gcaggaatac tcataaagtc ggaagaatat tccaagggtg
     4081 ttctccatgg aaattattca gaatgggacg atgcagggaa cctgattatt tcgggccaat
     4141 acaagaatgg tgaaaaagta gggaaatgga aagaatacaa gggagagaag gaagaggaaa
     4201 aggaataaag aattttggcc gcttgtgcat ggagctcatc attcatttta ctcacgttgt
     4261 ggcttgtatt tgatgggtgt ggagctctgc tccacaccca tcaacttttt tatcttctcc
     4321 caaaataaaa taccaaccat tcaagtttta tcccttcccc aaaaaatcta catagaccaa
     4381 aataaatatc gaattgcccc cttcaatcca tcctcctgag tggaggttac ctataaacta
     4441 tctgcaacgg tcattcacct tgctacacaa aatccatgat aaatttaaaa aaatccaagc
     4501 catgtttttg actcggatag atacaaaaag gggtactaaa agcttctcac ggtattatcg
     4561 tggccatatt cacctctctc gagcttatcc ttaagtttcg aaaatgcctc tatggtatag
     4621 gatacatctt cgagcgtatg gtcagcggtg ggaataaggc ggacgagaat ctgccctcgg
     4681 ggaatcaccg gataagttac gatgctacag aaaatatcga acgattcgcg catatcgtaa
     4741 ataagtccgg tagcctcgga tacgcttccc gacaaaataa ccggagtaac cggtgattga
     4801 gtattcccga tattgaaacc tgcattcaat aaaccttttt gaagttcatt tgcaataagc
     4861 cagagttttt ctcttaattc gggatgagta cggataattt cgagtcgttt caatgctcct
     4921 attaccatgg ccagaggcaa tgattttgca taggtttgcg aacgcatatt aaaacggaga
     4981 taatcggtaa ccaatttcga gcctgccaca aatcccccaa ttccagccat ggccttggca
     5041 aaggtgccaa aatagatatc tatgccctcc tgaactccca gatactgccc cgtaccagcc
     5101 cctgtttctc ccatcgttcc aaaaccatgt gcgtcatcca ccaaaagtct gaaagggtat
     5161 ttctttttta actctataat ttcgcagagt tttcccaaat cgcccgacat cccgtacact
     5221 ccttcggtaa tcaccaaaat tcctcctctg ttttcttcgg caagatgagc cgcccgctga
     5281 agttgttttt cgagattttc catgtgatta tgtggaaaaa caaaacgttt gccaagatgt
     5341 agtcttacac catctataat acaggcatga gcatcgctat cgtaaacgat tacatcctta
     5401 cggtcaacca atgaatcaat caccgaaatc attccctgat aaccataatt gagcacaaaa
     5461 gcgtcttctt ttcgaacaaa ttcggctaat tccctttcca gttgttcgtg ataaatcgtt
     5521 tgtcccgaca tcatccgggc tcccatagga taagccagac cccatctggc agcagcctcg
     5581 gcatccacct tcctaacctc aggatgattg gccaaggcaa gataattatt caaactccag
     5641 atgattcttt ccctgccacc aaaaatcatc cgatgaccaa tctccccttc gagtttcgga
     5701 aacatgtaat atccaaaagc atcctctcca tattgtccga gtggcccctt gtttgttgct
     5761 attttctcga atatatctct catataagtt tatctcttaa aaattggcaa aactatggtt
     5821 taattgccat ttttcaaaaa tccaaaatcc ttattcattg aatttgatta tcaattaatc
     5881 gattaaaccc atattgtgga ttaataactt caaaagaata tggccgaatg ggtgaagccc
     5941 gttacgaggc cattgaaaat ttagttgaaa atttctttat gagcaagata ctctaaaata
     6001 tctaatagaa acaacaattc ataatacgat ttttcatacc tttgcaccat gcaaaagaac
     6061 gcttacctga ttagctttat cttgctgact gtcattatat taacaggttg ttcgaagtat
     6121 caaaaaatat ataaaagtac ggataacgac ctgaaatatg aagcagccat tgcattttac
     6181 gagaaaaagg attattacag agctttgcag ctattcgacc aaatcatacc agctttcaga
     6241 ggtaccgaaa gagaggaaaa aatctcgtat tactatgcct atgcttatta ttatcaaaaa
     6301 gattatattc tggccggata ttatttcaaa cgttttgccg atacctaccc tacgagccgg
     6361 tataatgagg aatgcttgtt catgacggct tattgttcat atttgaactc tcccccctat
     6421 tatcttgacc agacaagcac agtggatgcc atacgcgatt ttcaggtttt catcaaccgc
     6481 tatcccacta gcagtcgagt tcccagggcc aatgaattaa tagaccagct caggttgaaa
     6541 ctcgaagaaa aatattacag aatttctcta ttatatttac aaatggagga atataaagcc
     6601 gccatagttt cgtttcaaaa cctgatgaaa gattatcccg atacgaaatt cagagaagag
     6661 gccatgtact atattgttga agcatggtat aaatatgccg aaaaaagtat acccgaaaaa
     6721 cgtatcgaac gacatcaggc agccattgat gcatttaacg atttcaaggc tcagtatccc
     6781 gaaagtagat atttacaaaa tgcacagaat ttgatcaaaa attcgtttaa aatagcaaaa
     6841 atcaaaattt gaatttataa ttctttaaat ttctttatgt tacataaata taagaggcta
     6901 catgccgata ccaccgccgt tactcgtcgt attaatgatt ttgatgcaca aacgggtaat
     6961 atctacgaaa cggtcgccat tctggccaaa cgtgccaacc agatcagcaa cgaattgaag
     7021 gaagcattgg atgccgaact aaaagctttt caaactacaa gcgaaaccct cgacgaagta
     7081 tatgaaaata aagaccagat tgaggtttcg tgcaagtatg aacaaatgcc aaagcctact
     7141 ttattagcca ttgccgaata tctcaacggc aaactttatt ggcgtaatcc tgtcaaagaa
     7201 atccaataat agagcctaat gctcgaaggg aaaaatattc tgattggcat cacgggtagc
     7261 attgccgctt acaaaatccc ttttcttatc cggttattga tcaagaaggg tgcgtgtgtt
     7321 caggcaattc ttaccccgac agcctgcaat ttcgttacac ctttaacgat atctactctc
     7381 tcgaggcgtc gagccatagt agatatgttc gaaaaagaat ctggcgaatg gaatagccat
     7441 gtagaattgg gtaattgggc cgatttgatg ttgattgctc ccacgtctgc caacacgatt
     7501 tcgaaaatgg cttcaggaca ggccgacaac ttgctacttt gtacttttat ggcagctcgt
     7561 tgccccgtat tcttcgcacc ggcaatggac gtcgacatgt atcagcatcc atctacacaa
     7621 gccaatatca gaaaacttca ggaaatcgga ttgcatctta ttgctccaca aacaggagaa
     7681 cttgccagcg gcctgacggg aaccggacga ctcgaagagc ccgaagtaat aatttctgtc
     7741 ctcgacgatt tctttaaaaa aaaaaccaca ctaaaggata gaaaagtcct gattactgca
     7801 ggtcccactc gagaacccat agatccggtg cgatatatca gcaatcattc ttcgggttta
     7861 atgggtttcg ctttggctga aaaagccgcg gaaatgggtg ccaaagtaac tttgattagt
     7921 ggtccggtaa aattggaaac aacccatcca gagattcgac gcataaatgt cgaaactgcc
     7981 cagcaaatgg cagaaaccac cctacattac ttttctgaat ccaacatcat tataatggct
     8041 gctgccatct ccgatttttc tccagttgaa ccctcccctg agaaaatcaa gaaacatgag
     8101 caggaaacac tggttttgaa tttgaaacct acaatggata ttcttgccga aatgggccgc
     8161 cgaaaaaaga atgatcagat cctagtgggg tttgctctcg aaacaaacaa cgaaaaagac
     8221 aatgccctct tcaaactaag aaataaaaat gccgatatca tcgttttgaa ctcgacacgc
     8281 gacaaagggg caacctttgg gtttgccacc aatcgtgtaa ctatctttca taaaaatggt
     8341 gatagggcac aatcagaact tctacccaaa acagaaattg ctcaaatgat tctcgatttt
     8401 atcattcaac atcattttta aaactattgc gtcaaatgcg tcgttttgga atttttatcg
     8461 gcattttact tttatccatt gcttcaattg gtcaggagct cgattgccag atttctgtct
     8521 cgacacgtca ggtagaaggc accgaccgtc aggtctataa caccttgcaa acggctttgt
     8581 tcgaattcat gaacaacaaa cgatggacca actatcaatt taaattggag gaacgtatcg
     8641 aatgcaccat gctcattcaa atctctgaaa ggctgtctgt agatgaattc aaaggaacca
     8701 tgaatgtggt tctccgcagg ccagtatata aaagcagtta caattccata ttactcaatc
     8761 atgtagataa agatattcaa ttcaattatg tcgaattcga acctcttgat tttcaggaaa
     8821 gtgccattac ttcgaatcta acggctatat tggcttatta cgcctacatc cttattggtc
     8881 tcgatttcga ttcgttttcg ttatatggag gaaccccttt ttatgaaaaa gctcagacca
     8941 ttgtcaacat ggcccagaac cttccacaaa aaggatggaa atcgttcgaa agcatgcgca
     9001 atcgttattg gctggcagag aatttcaaca atacaagtta taataaaatt aggcagttca
     9061 tttatcaata tcaccgtcag ggactcgact tgatggccga aaatgtggat cagggcagaa
     9121 cgaatattat cgagagcctc gaacttatgc gtcaggctta ccgcgataaa cccaattctt
     9181 tcctcttgca attaatccta gatgccaagc gtgacgaatt tatcaatata ttgaccggag
     9241 ctcccgatca ggataaggtg aaagcagtca atcttctcaa agaaatcgac cctgccaatt
     9301 ctacaaaata ccaaactgtc ctcacaaaat aatcttgcca caattcgaat ttcaaaatgc
     9361 tattcgtaat tttgaacaat taggaataac actatgcttt gcaggctgag cgtcaaaaat
     9421 tatgctttaa tcgaagaact cgagttcgaa cccgggccgg gattcaatat tattacaggc
     9481 gaaaccggtg caggaaaatc tatcttactc ggagctctgg ggcttattct tggaaatagg
     9541 gccgatatgc aggcattacg cgataactca caaaaatgta ttgtggaagg cactttcaaa
     9601 gtaaacctcg aacccgtaaa tcgattcctt tacgaaaaca atctggatgt ttgcgatgag
     9661 ctcatcatga gaagggaaat cctgccagga ggaaaatcac gggccttcgt caacgataca
     9721 cccgttaatc tcaatgcatt gaaaattatt ggagatatgt tggtcgacat tcattcacag
     9781 catgaaaacc tcctggtaaa tgaagcccaa ttccagcttg ccgttctcga cgactttgcc
     9841 cgtaataaag aaatcctatc gagttattat gcccaatatc tggattatcg caataaaacc
     9901 accaaactgg agttactcag cgaacaagaa gcaaaatcga tggccgaaca ggattttatt
     9961 cacttccagc tcgatgaact tcatgaagcc catatcgaac caggagaaca agctaagatc
    10021 gaaaacgaac tctctttact cacacatgca gaagaaatta aaactcgact gtcagaaatg
    10081 attcaaattc tggatgacga ggattcgggc gtaattcctg cacttgccag ccaattttcg
    10141 aacctgaaca gccttattcg tttcgatgaa gctctcaaag agatagaatc gagatacaca
    10201 agctgtttct atgaactcaa ggacatattg caggaattga ttaaatatgc cgacactata
    10261 cattacacac ccgataggat agcggctctc agtcagcgcc tcgatattct tttccgcctg
    10321 cagcaaaagc atcgcgtaaa agatgccgac gagctgatcg aagtaaaaga acatttcatc
    10381 gaaaaactga attcctatgc ttcgttgaat gaacagatag aatcgcttca acaagaaatt
    10441 atctggatag aaggagaact cgaagaaaaa gccaatcgat tatcagacag aaggaaggct
    10501 gctatacagc ccattcagca tcaattgaaa gaatcactct cagagctggg aatgcccgat
    10561 gccagcattg aagtagtttt agaatccaca accgatttta tcgaaaccgg gaaaaacaga
    10621 gtaaattttc tatttagtgc caatgtgggg caagaacctc gaagcatctc taaggttgcc
    10681 tcgggaggag agatttcgcg attgatgctg gctatcaaat cattgatttc gaataaaacc
    10741 atgattccgg ttctcatttt cgatgaaatc gacgccggag tttcgggtgg aatagcagcc
    10801 aaagcaggca ggctgatgaa acaaatggcc agaaatatgc aaatcattgc cattacacat
    10861 ataccacaaa ttgcggcact gggagacaac cattttctgg tatccaaaaa aataaataaa
    10921 ggcaccaccc agagcattat cgaacgactt tcaaccgaag gaagaatcag agaactggcc
    10981 gggatgctta gtaatcacga aattactcaa gaggccatag cggctgctaa acaattgttg
    11041 aagtgatttt gtttgaaaat tttaaacgaa atacatcgat caagtatgtc gtataattta
    11101 ctaaaaggaa agaaaggtat tattttcggc gcactcgacc ataagagtat tgcatggaaa
    11161 gttgccgaaa aggcctacga agaaggtgca cgcatcgtgc tcaccaacac tcccgctgct
    11221 attcgttttg gagaaatcga acaattggcc gaaaaaactc agagcattgt cattccagcc
    11281 gatgctacca atgtcaatga cattgaaaac gtctatcttc aaggaatgga atttttagac
    11341 gggaaaatcg attttcttct gcattcgatt ggaatgtcga tgaatgtgag aaaaaataga
    11401 ccctacgatg acgtggatta cgactatttc ttgaaaacac tcgacatttc tgccttatcg
    11461 ttccacaaaa tcgttcaagt ggccaagaaa ttggatgcca tcgaagaatg gggcagcata
    11521 gtagccttat catacattgc cgctcaacgg acgctctttc gatacaatga tatggccgac
    11581 gcaaaggcta ccctcgaatc gattgcccga agctttggat atatttacgg acgagaaaaa
    11641 aaagtaagga tcaatacggt ttcgcaatca cctacactta caaccgctgg aagcggtgta
    11701 aaaggaattg acgatttgta cgactttacc gacagaatgt ctccattggg aaatgcctcc
    11761 gccgaagaat gtgccgctta catcatcacc cttttctctg acctcacgcg aaaggtaacc
    11821 atgcaaaatc tatttcatga cgggggtttt tcgagcatgg gaatgagtgc ccgcgccatg
    11881 accatgtatt gccatagcct taaccctgaa gaatgaacat gtgaacccga cgaagaaacc
    11941 tgatacgaga attttttcta aattttgata agcatcagaa tgaattgctt aggaggttcc
    12001 gggatgctgg atatcaagta ccttttttat gcctaatttt cgtatcttgg aatccagagt
    12061 ttttggattt atacctaaaa gccgagctgc accatcttca ccactcacct tcccgttggt
    12121 caaacgaaga gcctctaaaa tatgctctat ctcaacttct ttgagggtct tcattgtttt
    12181 tctgagtttt tcgacttgaa acagagaggg cgatgttttg aaatcactaa aattaatata
    12241 tcacctgtag tcaaaataac tgccttttga atgatatgct taagttcacg aacattcccc
    12301 ggccattgat agttcatgag tttttgcatg tccatttcac ttatctttag gtagggctta
    12361 cccaacaccc ccccatgata ttcgatgaaa taatggataa gggccggaat atcttctaca
    12421 cgctccctca aaggagggat ataaatcgga aatgcattca aacggaaata aagatcgctg
    12481 cgaaatcttc cggcttttat ttcttcttcg agcttacgat t
//
LOCUS       DAOORU010000055        12472 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_6268_length_12472_cov_5.721591, whole genome shotgun sequence.
ACCESSION   DAOORU010000055 DAOORU010000000
VERSION     DAOORU010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12472)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12472)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12472
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6268_length_12472_cov_5.721591"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            383..1855
                     /locus_tag="PLF20_05030"
     CDS             383..1855
                     /locus_tag="PLF20_05030"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HOX80401.1"
                     /translation="MLKRILFGIASAILVQGLNIPLYSTDLSSKPKAKYVFYFIGDGM
                     GLAQVSAAEAFLATNNPQIQLKSLHMSNFPVIGLSTTYAANRLITCSAAAGTALASGF
                     KTSINTIGMDASRTASLQSVAAMAKAKGMKVGIITTVCINHATPAVFYAHRPDRNDYY
                     TIALQLPESGFDLFGYGGIKDAKGENGDRPDAYDIAVTKGYKIIRGQKDFLSLQELPG
                     KIIVLNDRLDEGKAVPFIIDQTPQDMPLSQFVEKSIQLLDNPEGFFMMVEGGLIDWAC
                     HSNDAATAIKEVIDLDKAIGAALDFMKLHPNETLIVVTADHETGGMAMGNALMKYESN
                     FNLLSSQKVSESVLKKHINDFRETKCKDGCQFEEIFPLLNKYTDLGLNIPLTGFDSIQ
                     LKMAFETSMLRKIPFSEKDGIYLLYGDEEPLSVTAVKMISQKAGIGWTTWSHSGIPVP
                     VRAKGVNQEKFGGYFDNTKIPELILEAMGISSEIFMRDGK"
     gene            complement(1976..2830)
                     /locus_tag="PLF20_05035"
     CDS             complement(1976..2830)
                     /locus_tag="PLF20_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOX80402.1"
                     /translation="MKKLFFIFCGFLVLTSFSTDKPAWKLFDKSGKECNYQNLLDEAS
                     KADVVLFGELHDNPISHWLQYELLSDLYRMKGAQLVTGAEMFETDNQLLLDELIAGQI
                     SLKNFEAEAKLWPNYSTDYKPMVEFALEHRIPFIGTNIPRRYAAMVNRKGFEALDGLS
                     NQAKMLIAPLPVQYDSALPAYKAMLNMGGHLGANLPKAQAIKDATMAWSILKYLQAGE
                     IFLHLNGAYHSDNYEGIVWYLRKYRPGLHILTITTIEQANMDSLLEENLGKANFTILV
                     PQTMTKTH"
     gene            complement(2850..4787)
                     /locus_tag="PLF20_05040"
     CDS             complement(2850..4787)
                     /locus_tag="PLF20_05040"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOX80403.1"
                     /translation="MMERIKNWITRHPYDALFILVILAGAVLRLYDFSCWSLSNDELS
                     ALNRLRSDSFSELIQNGVRPDGHPAFTQVFLYYWTAIFGLQPFWVRLPFVLAGIASIW
                     LVYAIGKQWFNAATGLYASLFIAVLPFTILYSQLARPYSFGLLFTLLTAYFWGRYFLL
                     HHYGWGTLAGFALSAAFAMYTHYFSFMMAGLFILAGFLFISRDNGKGFLISIVTIFLL
                     YLPYLNIFLFQLSLGGVGGAQGWLTKPKWDWIFNFFYYAFGLSAFHLLLLLAFLTIFM
                     VWYVKNTHIRKIQWVLLAIFLLPYLIGFVYSILVNPVLQYSGLLFAFPFLLLFLFSFF
                     DDDRPVVSSVFVLVGLIGGSWVALDKTDDFRNSQFADFKACTSVYMEWENKYRKDSIT
                     LTASINHPYYLEYYFHNAHKNPPQIIVLESKQGRELADLVQILEQSRTPYFAYFRLKP
                     APIEIPDVIAGTYPVLLDYRDYFGQAEAFLFAKDTSLKGIPKPKPISNFYCDFEGDDE
                     LFGVRISNLDTICCSGKYAMHLRPEMEWGPGIKITTRQAELRSPSRVRIKVEVLTDST
                     LIDSPIVLTLTDQFDKQYIWLSGKLENFLTPGKWGTAYFTADLPHPYSLGDELKIFIW
                     NKDHRNLWVDNFSIEFYKLEK"
     gene            complement(4788..5540)
                     /gene="rlmB"
                     /locus_tag="PLF20_05045"
     CDS             complement(4788..5540)
                     /gene="rlmB"
                     /locus_tag="PLF20_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOX80404.1"
                     /translation="MKSNDKQIIFGLQPVKEAVKAGKDFERLYIYKGMNKENFSELKI
                     LLQQYHIAYQLVPIEKLNRMVQGNHQGVVGIISRIVYHQLEDIIAEVFEKGRSPFIVI
                     IDRVTDVRNLGAIARTAECAGVDALVVPLHESAMIHAEAIRTSSGALARIPICRVDNL
                     KWAVNYMKASGLSIISISEKGNCSFSEINFTIPVALILGSEEDGISNALLRLSDEICF
                     VPMMGKIGSLNVSVAGGIAMYEVVRQRINKNQ"
     gene            complement(5657..6889)
                     /locus_tag="PLF20_05050"
     CDS             complement(5657..6889)
                     /locus_tag="PLF20_05050"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOX80405.1"
                     /translation="MKKKRKIKVSVISSILLLILQGSCTSSKLLQNNNFASDMLAGSM
                     KALTGIQVYGANPDSIHLYLKIDPSLLKPQALPDGLEFLSLSLRWMIFSSFSEKSIID
                     SSTFRTGPLSPHQSEKLIFEHSLKAPRGKSYYLCYKPSSDRYRNWNIMWIDRTNEQSH
                     NHFLITLSDSTLIFRSEELFDKPLYIYHSVPTDNTNLFLRRINLCSCTPSPPFALKVG
                     ACKIRFDSIKTIRIHHGKSEEFLLENPGLYFIQIDTNLIGAGSFLVLNPEFRHLLIKA
                     SSRYITDNDEFRDIWLGNIGLLDFWENIAGSTQRTIELSKRFSYAVNQSNLLFTRALP
                     GCLTDRGMIYIVFGPPNAIYEDNELETWVYREFRQKRDLIFNFKKEWIFPIGLEYRLD
                     RRAEYTQIWYEAIERWRR"
     gene            complement(6867..8231)
                     /gene="rmuC"
                     /locus_tag="PLF20_05055"
     CDS             complement(6867..8231)
                     /gene="rmuC"
                     /locus_tag="PLF20_05055"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOX80406.1"
                     /translation="MGINIIDIFLTGLGVLIGVLVTGFYFIRKQKKLREGFIEKQKEE
                     ARLQSELDIRNRWYEEIKQEYEKRMQSLSAELDSSRIEASNLKQNLVSLQEKNAYLTQ
                     MKDEAQALYEKFKAEFEALNTKFLKENAEALGEESRKTVKELLDPLKERLETFDRKIS
                     ETSALQSEKQGELKGIIEKLFEQNRNLSEEADRLAKAIKGDVKKLGNWGELVLERVLE
                     ISGLEKEREYVMQWQSSNIEGTALRPDAIILLPDNKQIIIDSKVSLSAFDQYLRAETE
                     DEASLHAQKHVVAVKTHIQNLSSKQYQNLNDLHSTDFVLMFIPIEPALALALSTEPDL
                     YSYALEKHIILVSPTTLLATLRTVEMIWRQEKQQKNIKEIIRLASNMYDKLALLVKKL
                     EELGRNIRKSSDLYDDVMRTLRTGKGNLMQQAFKMQELGTPVTKKLSPPNETLLFDEE
                     ETEN"
     gene            8275..9066
                     /locus_tag="PLF20_05060"
     CDS             8275..9066
                     /locus_tag="PLF20_05060"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOX80407.1"
                     /translation="MKSFRWLSLLTSLFFVWGKFLFAQQAMPAMGKTELREFIKNQMI
                     YPEQALSLGLYGKVELLCVIDADGNLIKSRSIEMTDKDLIREGKRLARGIVWNPARER
                     NKPTEGEYILEIEFHPKKYSKWVKHRGWDRPDADWDTSMQIFSYRNLTQLAKPVLPEG
                     ATFADFIEKNIHTPDAAKRMGISGKVVLSFIVESDGSSSNFIVREYLGMGCTEEAIRV
                     MKLLRWQPARKNEKAVRSWNSTTLWFGEGQPQYNLQPGYNPGSML"
     gene            9175..9248
                     /locus_tag="PLF20_05065"
     tRNA            9175..9248
                     /locus_tag="PLF20_05065"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9209..9211,aa:Asp,seq:gtc)
     gene            complement(9432..10253)
                     /locus_tag="PLF20_05070"
     CDS             complement(9432..10253)
                     /locus_tag="PLF20_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOX80408.1"
                     /translation="MNLIEPFGYVFFQHALLASVFSAIICGFIGAYIVSRRMVFISGG
                     ITHASFGGMGLAYFFGFNYLLGAAVFALVSALVVEYLSKRTEVREDSAIGMLWSLGMA
                     IGIIFTFLSPGYAPNLMSGLFGSILAVSNTELWLMAVLAIIIILFFIQFFPAILAVAF
                     DMEYASTHHIPVNFINYSLISLIALAIVLNIRVSGIILVLAMMTIPPVTANLLTKRLK
                     PMILLSIALGLAGNVLGLIFSYLLNLPSGATIIVTHILIFGIVRLCISLRETHTA"
     gene            complement(10240..11028)
                     /locus_tag="PLF20_05075"
     CDS             complement(10240..11028)
                     /locus_tag="PLF20_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOX80409.1"
                     /translation="MTRNKLIEISDLSAGYYGKPVIYHIHLTVYEGDFIGIIGPNGGG
                     KTTFIKTIAGIIKPLSGMLIFYSPEGQAIEKPKLGYLPQVNTFDKSFPISVEEVVLTG
                     LLSAKGRLRRFRANDKEAVHEVLERLKISHLASKPIGELSGGQMQRVFLARAIVSQPW
                     ILLLDEPSTYADNRFEAELYELLVQLNEKMTILMVSHDLGMIPSYIKSIACVNGTLHY
                     HPTPEITPEILRVYNCPIELIAHGIVPHRVLSTHQENSSTNESH"
     gene            complement(11086..11994)
                     /locus_tag="PLF20_05080"
     CDS             complement(11086..11994)
                     /locus_tag="PLF20_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HOX80410.1"
                     /translation="MEKHHLYIISAFILICVFSSCRHSNTINESAIAVSILPQKYFVE
                     QIAGNDFDIQVLIEPGASHEMYDPTPAQLARMASTLIWFKNGFLGFEIQYEDKFRELN
                     PKLKVVDLSKGVQYIVENYGEESHETESSIEEHRGVDPHYWLSVSGARIIAQNIYEEL
                     IRDHPDNHEEYNKNLQNFQQRLDSLEAYIHQRLDSVSQHSFMIFHPSLTYLARDFNIQ
                     QIAIEERGNSPSTRRMKEIVELARSKGITMILYQSQFDSGNAATIAKEIGGTAVPFDP
                     MAYDWYQNMIKLTDLLYQGLNPSNKK"
     gene            complement(12292..12432)
                     /locus_tag="PLF20_05085"
     CDS             complement(12292..12432)
                     /locus_tag="PLF20_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80411.1"
                     /translation="MSKSGGAFDSPSPGYNYMHEDYVKLKMMNLMLHKEAARPVLDHV
                     IK"
BASE COUNT         3860 a         2470 c         2488 g         3654 t
ORIGIN      
        1 taggaccaat caggttgttc gaataaaatc ttaaaatctt caaataaacg catgggatta
       61 tttttttaat aattttcaac aaatttagaa taaaaaaata ataccaataa cagttttaaa
      121 taattagtta tcaaaaagtt attaacaatt tgagtttatg gacagactct cattagtttt
      181 ataaaccctg tttgtttctg atgataaatt ataggttaaa ttgatttatc agttaattga
      241 atatggtgat cgagcagatt gaaaattttg ttttttatcc atagcttcat attttcccct
      301 ttcctctgtt caagttcaat tgtagattta aaaagatcaa gttcaatatt tttacttttg
      361 caatgattaa aaacattcga ttatgctcaa gagaatactt tttggaattg cttcggctat
      421 actggtgcaa ggcctgaaca ttccactata ctccaccgat ttatcttcga aacccaaggc
      481 caaatatgta ttttatttta ttggagatgg gatgggattg gcacaggtga gtgctgcgga
      541 ggcattttta gccaccaata atccacaaat tcaattgaaa tccctccata tgagtaattt
      601 tcccgtaatt ggcttgagta caacctatgc tgccaatcgg ttgattactt gttcggctgc
      661 tgcaggtaca gcgctggctt caggtttcaa gacttcgata aatactatag ggatggacgc
      721 ctcccgaact gcttctttgc aatctgttgc tgccatggca aaggcaaaag gaatgaaagt
      781 aggtatcatt actacggtat gcattaatca tgcaactccc gcagtttttt atgctcatcg
      841 tcctgatcga aacgattatt atacaatcgc ccttcaactg cccgaaagtg gtttcgattt
      901 gtttggatat ggaggcataa aggatgctaa aggtgaaaat ggtgatagac ccgatgcata
      961 cgatattgcc gttacaaaag gctataagat catccgaggg cagaaggatt ttctatcact
     1021 gcaagaactt cctggaaaga ttatagtcct gaacgatcgt ttggatgagg ggaaagccgt
     1081 tccatttata atcgatcaaa ctcctcagga tatgcctctt tcgcagtttg tcgaaaaaag
     1141 tattcagttg ctcgataatc cggaaggttt ctttatgatg gtggaaggtg gactgattga
     1201 ctgggcttgc catagcaacg atgctgctac tgcaattaaa gaagtaatcg atttagataa
     1261 agccattggg gctgctctcg attttatgaa acttcatccc aacgaaaccc ttatcgtggt
     1321 tacggccgat cacgaaacag gcggcatggc catggggaat gcattaatga aatatgaaag
     1381 taattttaat cttttatcca gccagaaagt ttcggaatct gttttaaaaa agcacatcaa
     1441 tgactttcgg gaaacaaaat gcaaggatgg gtgtcagttc gaggaaattt tccctcttct
     1501 caataaatat acagatctgg gattaaatat tccattgacg ggtttcgaca gtattcaatt
     1561 aaaaatggcc tttgagacaa gcatgctaag gaaaatacct ttttctgaga aagatggcat
     1621 ttatttgctc tatggtgatg aagagcctct ttcggtaact gccgtaaaga tgatttcaca
     1681 aaaagctgga attggatgga ctacatggtc gcatagtggc attccggttc ctgtgagagc
     1741 aaaaggtgta aatcaggaaa agttcggtgg ttattttgac aatacaaaaa tacctgaatt
     1801 gatcctcgaa gccatgggta tttcttccga aatattcatg agagacggaa agtaattttt
     1861 ttaagcttgt ctgaaataag ttggctatgc catatatcta tacaactttg aaagggccca
     1921 taacccgcat cctgagcatt aaaccacatc cactattatg gaattttagt gcgctttaat
     1981 gcgtttttgt catggtttgg ggaactaaaa tggtgaaatt ggccttgccg agattttcct
     2041 ccagcaagct gtccatattg gcttgttcga tagttgtaat agttaggata tgcagacccg
     2101 ggcgatactt tcgaagatac cacactattc cttcataatt gtcggagtgg taagcaccat
     2161 ttaaatgcaa gaagatttca ccggcttgca gatatttcag aatggaccat gccatggtag
     2221 catctttgat ggcttgagct ttgggaagat ttgctcccag atgtcctccc atatttagca
     2281 tagctttata ggcaggcaag gcagaatcgt attgaacagg taagggagct atcaacattt
     2341 tggcctgatt acttaaacca tcgagtgctt cgaatccttt tcgatttacc atagcagcat
     2401 acctgcgggg aatattcgtc ccaatgaatg gtattcgatg ttcgagtgca aattccacca
     2461 tgggtttgta atcggtcgaa taatttggcc acaatttggc ttcggcctcg aaattcttca
     2521 agctgatttg ccctgctatc aattcatcga gcagaagctg attgtcggtt tcgaacattt
     2581 cggcaccagt aacaagttga gcacctttca ttctgtacaa atcagataac agctcatatt
     2641 gaagccaatg gctgatggga ttatcatgca actcgccaaa caataccacg tcggctttcg
     2701 atgcctcatc caacaaattt tgataattac attccttgcc cgatttatcg aataatttcc
     2761 atgcaggttt atccgtcgaa aaagaagtga gaaccaaaaa tccacaaaaa atgaaaaata
     2821 atttcttcat gtttagtttg ttttttaatt catttttcaa gtttataaaa ttcgatcgaa
     2881 aaattatcaa cccatagatt tctatggtct ttattccaga taaaaatttt cagttcatcg
     2941 cccaaggaat aaggatgagg caaatcggca gtaaaatagg ctgtgcccca tttacctggg
     3001 gttaagaagt tttcgagttt accactaagc caaatgtatt gtttgtcaaa ctgatcggta
     3061 agtgtaagaa caatgggcga atcgatgagc gtactatctg ttaggacttc cacttttatt
     3121 cttactcttg aaggtgagcg aagttcagcc tgtcgggtcg taatttttat acctggcccc
     3181 cattccattt caggacgcag atgcatggca tattttccac tgcaacagat agtatcgaga
     3241 ttggatattc taacaccgaa aagctcatcg tcgccttcaa aatcacagta aaaattactt
     3301 ataggcttgg gtttggggat gcctttcaag gaagtatctt ttgcaaaaag aaatgcttcg
     3361 gcttgaccga aataatctct gtaatcgagc agcacaggat atgtacctgc aataacatct
     3421 ggaatttcga tgggtgcagg ttttaaacga aaataagcaa aatagggtgt tcggctctgt
     3481 tctaatattt gtacgagatc agccagttca cggccttgtt tcgattccag aacaataatt
     3541 tgaggagggt ttttatgcgc attgtgaaaa taatattcga gataataggg atgattgatg
     3601 gatgcggtaa gggtaatgct gtctttccgg tatttatttt cccattccat gtaaaccgaa
     3661 gtgcaggcct tgaaatcggc aaattgtgaa tttcggaaat catcggtttt atccagtgct
     3721 acccatgaac ctcctatcaa acctactaaa acgaagacag aagaaaccac agggcgatcg
     3781 tcatcgaaaa atgagaataa aaacaaaaga agaaatggga aagcaaacaa aagaccgctg
     3841 tactgcaaaa ccggattgac aagaatgcta taaacaaaac ctatcagata cggcaataag
     3901 aatatagcca gtaaaaccca ttggattttt cggatgtgtg tattttttac ataccatacc
     3961 ataaaaatcg tcagaaaagc cagtaacaat aacagatgaa aagcagaaag tccgaatgca
     4021 tagtagaaga aattaaaaat ccagtcccat ttaggtttgg ttagccatcc ctgagctcct
     4081 cccactccgc caagcgataa ttgaaacaag aagatgttca gatatggaag ataaagtaaa
     4141 aagatagtta ctatcgaaat gagaaaacct ttcccgttgt ctcgggagat gaataaaaat
     4201 ccggctaaaa tgaagaggcc ggccatcata aagctaaaat aatgggtgta catggcgaat
     4261 gcagccgaaa gtgcaaaacc ggccagagtg ccccagccat agtggtgaag aagaaaatat
     4321 cttccccaga aataagctgt aagcaaggta aataataagc cgaagctata gggccgtgca
     4381 agctggctgt ataaaatcgt gaaaggcaaa acggctataa atagagatgc atataggccg
     4441 gtagctgcat tgaaccattg tttgccaatg gcataaacca gccaaataga agctattcct
     4501 gccaaaacga agggtagtcg tacccagaaa ggttgcaaac caaaaattgc cgtccagtaa
     4561 tacagaaaaa cctgtgtaaa ggccgggtga ccatcaggac ggacaccatt ctggataagc
     4621 tctgagaaac tgtcggaacg caggcgattc aaggcactta attcatcatt actcaacgac
     4681 cagcagctaa agtcatatag cctcaatacc gctcctgcca aaatgaccag aataaacaat
     4741 gcatcgtaag gatgccgtgt aatccaattt tttatccttt ccatcatcta ttggttttta
     4801 tttattcgtt gacgcacaac ttcatacatt gcaatacctc ctgctacgga aacattcaac
     4861 gagccaatct ttcccatcat cggaacaaaa caaatttcat ccgacaagcg aagcaaagca
     4921 ttgctgatac cgtcttcttc actgcctaaa ataagggcta ccggtatggt aaaatttatt
     4981 tccgagaagg aacaattccc tttttctgag atggaaataa tggataaacc acttgctttc
     5041 atataattta ccgcccattt cagattatct accctacaaa tgggaatgcg tgccaatgca
     5101 cccgaagaag tcctgatggc ttcagcatgt atcatggccg attcatgcaa gggaaccaca
     5161 agagcatcta caccagcaca ttcggcagtt ctggctatgg caccaagatt cctcacatcg
     5221 gtaaccctgt cgatgattac aataaaagga gaacggccct tttcaaagac ctcagcaatt
     5281 atatcttcga gctgatgata aacgattcga gaaattatcc ctactacacc ctgatgattt
     5341 ccctgtacca tgcggttgag cttttcgatg ggaaccaatt gataggcaat gtgatattgc
     5401 tgcaacaata ttttcaactc cgagaaattc tctttgttca tccctttgta tatatacagc
     5461 ctttcgaaat ccttaccagc ctttacggcc tcctttacag gctgtaatcc aaaaataatc
     5521 tgcttgtcat tcgatttcaa gtgaataaag tattttagat aaaaatttta tagaattcga
     5581 ttcaaaaaat gaaagctttg tttcatttat ttcgggcaca aaattgcaaa aagacctcga
     5641 aaggtgaata cttaatttat cttctccatc tttcgatggc ctcgtaccat atctgagtat
     5701 attcagccct gcgatcgagc ctatattcca gaccaatggg gaatatccat tcttttttga
     5761 aattgaagat gagatctctt ttctgccgaa attctctgta aacccaggtt tcgagctcat
     5821 tatcctcgta gatggcattg ggaggaccga atacgatgta gatcatgcct ctgtcggtga
     5881 gacaacctgg taaggctcgc gtaaaaagaa gattggactg gtttacggca taactaaatc
     5941 gtttcgagag ttctatagtt ctttgagtgc ttccggcaat gttttcccaa aaatcgagca
     6001 aaccaatatt ccccaaccaa atatccctaa attcatcatt atcggtaata tatcttgacg
     6061 aggctttaat gagcaaatgt cgaaactcag gatttaatac aaggaaagag ccggctccaa
     6121 tgagattggt gtctatctgg ataaaatata accctggatt ttcaagtaag aattcttccg
     6181 atttgccgtg atgaattctt atggttttta tcgagtcgaa ccgaatcttg caagcgccga
     6241 ctttgagtgc aaagggtgga gagggagtgc atgaacataa atttatcctc ctgaggaata
     6301 aatttgtatt atctgtagga acggaatggt agatatacaa gggtttatcg aataattctt
     6361 ccgaacggaa tatcaaagtg ctatcggata gggtgatgag aaaatgatta tgactttgct
     6421 cgttagttcg gtcaatccac ataatattcc aatttctgta cctatcggaa gaaggtttat
     6481 agcataaata ataggatttt cctcgaggcg ctttcaagga atgttcgaat atgagttttt
     6541 cactttgatg gggactcaga gggcctgttc gaaaagtgga agaatcgata atggattttt
     6601 ccgagaaaga tgaaaaaatc atccatcgaa ggctgagcga aagaaattct aagccatcag
     6661 gtaaggcttg cggtttcaac aaagatggat cgattttgag ataaaggtgt atactatctg
     6721 gattggcacc atagacctgt atacctgtta atgccttcat agacccggcc agcatatccg
     6781 aagcaaagtt gttgttttgt aagagtttgg aagatgtgca cgatccttgc aaaatcaata
     6841 acaagatgga tgaaattaca cttaccttaa ttttccgttt cttcttcatc gaacaagagc
     6901 gtttcgttag gtggacttaa ttttttcgta actggggttc ctaattcctg cattttaaaa
     6961 gcctgttgca ttaaatttcc cttcccagtt ctcaaagttc gcatcacatc atcgtataag
     7021 tcggaagatt tccgaatgtt tctgcccaat tcttcgagtt tctttaccaa taaggcaagt
     7081 ttatcgtaca tgttcgaggc taagcggata atttctttta tatttttttg ttgtttttct
     7141 tgacgccaga tcatttccac tgttcttagc gtggctaaga gggtggtggg gcttaccaga
     7201 ataatatgtt tttcgagagc ataagaatag agatcaggct cggtggagag agccagtgca
     7261 agagcgggtt cgatgggaat aaacatcaac acaaaatctg tcgaatgcaa atcattcaaa
     7321 ttttggtatt gttttgagct taagttctga atatgtgttt ttacggccac aacatgtttc
     7381 tgggcatgaa gggatgcttc atcctcggtt tcggcacgta gatattgatc gaaagcagaa
     7441 agcgaaactt tactatctat aataatttgt ttattatcag gaagcaggat aatggcatca
     7501 ggccgtaagg cagtgccttc tatattggac gattgccatt gcataacata ttctctctct
     7561 ttttcgagac ccgaaatttc caggaccctt tctaaaacca gttcacccca attccccaat
     7621 ttttttacat cacctttgat agccttggcc agtcggtcgg cttcttcact caaatttctg
     7681 ttttgctcga agagtttttc gataattcct ttcagctctc cctgtttttc actttgcagg
     7741 gcagaggttt ccgaaatctt ccgatcaaag gtttccaaac gttctttaag tgggtccaaa
     7801 agttccttga ccgttttgcg gctttcttcg ccaagagctt cggcgttttc ttttaaaaat
     7861 ttcgtattca gtgcttcaaa ttctgcttta aacttttcat ataaagcctg tgcttcatcc
     7921 ttcatttgtg ttaaatacgc attcttctcc tgtaaactca ccaagttttg cttcagattg
     7981 cttgcctcaa ttcgggatga atccaattca gccgaaagag attgcattcg tttttcatac
     8041 tcctgcttaa tttcttcata ccaacgattt cttatatcga gttcggattg taaacgcgcc
     8101 tcctcctttt gcttttcgat aaaaccttct cttaacttct tttgtttgcg gatgaaataa
     8161 aatcccgtaa ctaaaacgcc aatcagcaca ccaaggcctg ttaaaaatat atcgataata
     8221 tttattccca tcacaaaaat tgactaatat tgtaagaggt aaaattaaat aaaaatgaaa
     8281 tcatttcggt ggttaagttt actgacaagc cttttttttg tttggggcaa gtttttattt
     8341 gcgcagcaag ccatgccagc gatgggcaaa acagaattaa gggaattcat taaaaatcaa
     8401 atgatttatc ccgaacaggc tctttcgttg ggcttgtatg ggaaagtgga attgctttgt
     8461 gtcattgatg ccgacgggaa tctgataaaa tcccggtcta tcgaaatgac tgataaagac
     8521 cttataagag aagggaagag gttggcaaga ggaatagttt ggaatccagc gcgtgaaaga
     8581 aataaaccga ctgaaggaga atatatttta gagattgaat ttcatccaaa gaaatattct
     8641 aaatgggtga aacatcgagg gtgggacagg cccgatgccg attgggacac ttccatgcag
     8701 attttttctt acagaaacct cacccaactg gcaaagcccg ttttgccgga aggagctaca
     8761 tttgccgatt ttatagaaaa aaacattcat actcccgatg cagcaaagcg tatgggaata
     8821 tcgggaaagg tagttttgag ttttattgtc gaatccgatg gaagttcgag taattttatc
     8881 gtaagggaat atctggggat gggatgtaca gaggaggcca tcagagtgat gaagctatta
     8941 cggtggcaac ccgcccgaaa aaacgaaaaa gcagtaagga gttggaatag taccacgctt
     9001 tggtttggag aaggtcaacc tcagtataac ctgcaacctg gctataaccc tggctcgatg
     9061 ctttgaccat ggagaagcga aatgaagaat ttcgataaaa ttttaccgaa attattggca
     9121 attccgatag aagtattact tttgcaagcc caaggtattg atataatctt tcaaggtctg
     9181 gtagttcagt tggttagaat gccgccctgt cacggcggag gtcgcgggtt cgagtcccgt
     9241 ccagaccgca aaatccccga ggattttcga gggttttttg tctcatctct gaaatacttg
     9301 acaggtaagc cttcttgaaa taattatcat tgatttgact ttttaaccac attgaataag
     9361 taaaaaattc taaggtcttt acagaaattc aatgagcagg agaaaaatcc gggtagcaag
     9421 tttttgaaat tctatgctgt gtgggtttct cgtaatgaaa tacaaagcct tacgattcca
     9481 aaaatcagga tatgcgtgac aataatagtt gctcccgaag gaagattcaa caggtaggag
     9541 aaaatcaggc caagtacgtt tccagcgagt cctaaagcta tggataacag aatcatgggt
     9601 ttcaatcgtt tagtcagcag attggccgtt acaggaggaa tggtcatcat agccagaacg
     9661 agtataatgc cagaaactct gatattaagt acaatagcca gggcaatcag cgaaatgagc
     9721 gaataattga tgaaattcac aggaatatga tgggtactgg cgtattccat atcaaaggcc
     9781 acggccagta ttgctgggaa aaactgtata aaaaacaaaa taataatgat ggcgagcaca
     9841 gccatcagcc aaagctcggt attgcttacc gcaagaatgc ttccaaacag acccgacatc
     9901 agattgggag cataaccggg cgaaagaaac gtaaaaataa ttcctatggc cattcccaac
     9961 gaccataaca tgccaatggc cgaatcttct cttacctcag ttcgtttgga cagatattca
    10021 acaaccaaag cagaaacgag tgcaaaaaca gccgccccaa gcagataatt aaaaccaaag
    10081 aaataggcca aacccattcc accaaacgag gcatgtgtga tgcctccact gatgaaaacc
    10141 atacggcgtg aaactatgta agctcctata aaaccacaga tgatggccga aaagaccgag
    10201 gctaaaagtg catgttgaaa aaatacgtac ccaaaaggtt caatgagatt catttgtaga
    10261 agaattttcc tgatgtgtgg atagaaccct gtgcggaaca atcccatggg caatcaattc
    10321 gataggacaa ttgtaaactc tcaaaatctc gggtgtaatt tcaggagttg ggtgataatg
    10381 cagcgttccg tttacacaag ctatcgattt tatataggat ggaatcattc ccagatcatg
    10441 ggagaccata agaatagtca tcttttcgtt gagctgaact aataactcat acagctcggc
    10501 ttcgaatcga ttatcggcat aagtggaagg ctcgtcgaga agtaaaatcc aaggttgcga
    10561 aacgatggct ctggctaaaa atacacgctg catctggcca cctgataatt ctccaatagg
    10621 tttcgacgcc aaatgactta tctttaatct ttccagaact tcatgtacag cctccttatc
    10681 atttgctcta aaacggcgca atcttccttt agccgacaat aaacccgtaa gaacgacttc
    10741 ttctaccgat atgggaaacg acttgtcgaa agtattcact tgtggcagat aacccaattt
    10801 gggtttttct atcgcttgcc cttcaggact gtaaaatatg agcatcccac tcaaaggttt
    10861 tatgatgcct gcaattgttt ttataaaagt agttttccca cccccatttg ggccaattat
    10921 accaataaaa tcaccttcat aaaccgttag gtgaatatga taaatcactg gtttgccata
    10981 atatcctgcg ctcaggtcgc taatttctat taacttattt cttgtcaaaa tttataggcg
    11041 ttttatttca atgaattgat ttttcctgaa ttaatcgtaa ttgtatcatt ttttattaga
    11101 tgggttcaaa ccttgataaa gcaagtcggt caatttgatc atattctgat accagtcgta
    11161 tgccatagga tcgaaaggaa ctgctgtacc gccaatttct ttggctatgg ttgcagcatt
    11221 tcccgaatcg aactgacttt gatagagtat catagtgatg cccttgctgc gggctaattc
    11281 tacgatttcc ttcattcttc tggtcgaagg agaatttccg cgttcttcaa tagcaatttg
    11341 ttggatatta aaatctctgg ccagataagt taaagaagga tgaaaaatca tgaagctgtg
    11401 ttgtgaaaca ctatcgagcc gctgatgtat gtaagcctcc agcgaatcta aacgctgctg
    11461 aaaattctga agatttttat tatactcctc atggttatcg ggatgatccc ttatgagctc
    11521 ttcgtaaatg ttttgagcta ttattcttgc accacttacc gataaccagt aatggggatc
    11581 tacacctcga tgctcttcga tggaagattc tgtttcatga ctttcttctc cataattttc
    11641 gacaatatat tgtactcctt tggacagatc gacgactttc aattttggat ttagttctcg
    11701 aaatttatct tcgtattgaa tttcaaaacc taaaaaacca ttcttgaacc aaattaaagt
    11761 actggccata cgggctaact gagccggagt gggatcatac atctcgtggc tggcacctgg
    11821 ctctatcaga acttgaatgt cgaaatcatt ccccgctatt tgctctacaa aatatttctg
    11881 tggcaaaatg ctcacggcta ttgccgattc atttattgtg ttagaatgtc tgcaagacga
    11941 aaagacacaa atcagtataa atgcacttat aatgtacaga tgatgctttt ccatatcatt
    12001 catattcatc agcaaattta cgaaggttaa ggacaaatta caacaaataa caaacaacga
    12061 acaacttacc aaattacaaa ctccaaatta caaattacga acaaactcca acgaacaatg
    12121 aacaactgac aaccagtaaa tcccaaatta caagcaaatt ccaaattcta attaccaatc
    12181 accatccact atccaccatc caccattctc cgttcccccc tcggcattca ccataattag
    12241 tccgaaaacg gttaaaatat taaagcattg atacttggat agggggaact gttatttaat
    12301 gacatgatcg agtaccggac gagcggcttc cttgtgaagc attaaattca tcattttgag
    12361 ctttacataa tcttcgtgca tgtaattata tcccggtgaa ggagaatcga aagcacctcc
    12421 tgacttcgac acattttaag agacattttt acaacaatca cacaatgaga ta
//
LOCUS       DAOORU010000056        12419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_6298_length_12419_cov_4.252346, whole genome shotgun sequence.
ACCESSION   DAOORU010000056 DAOORU010000000
VERSION     DAOORU010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12419
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6298_length_12419_cov_4.252346"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="PLF20_05090"
     CDS             complement(<1..1289)
                     /locus_tag="PLF20_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOX80412.1"
                     /translation="MKRLLLFMVILLTTSSFILAQNRIEGKVTDAKSGEALPGVTIYI
                     KGTTIGTVSDVNGKYVLESAALKPQAVIVFSFVGYNTQEVVFQNQKVLDIKLEPSQVM
                     LEELVVIGYGTTKKRNVLGAISKVGTQELTRLPVAGIDQALQGAAAGVQVTQNTGAPG
                     EGVSVRIRGIGSINSSNDPLYIVDGIPTADALNVIAPGDIENITILKDASSAAIYGAR
                     ANNGIILITTKKGKEGKPVLQYHGQSGVQAVGHIPKMVNTADYITIYNEAATNDNKYL
                     PEGLQRVLITPQLAANFVDINYLEELFRIAPINSHELSYSGSTGKTSYFMSGNFFDQR
                     GVIENTGYTRVSSHLSLETEIFPWLKTGMSMIVGHSKTDIIGSSGDGYGGNGGSVIRY
                     AFFRNPAIPIKFPDGRWVDRPAEYFGDQIYDSFLGDG"
     gene            complement(1304..3040)
                     /locus_tag="PLF20_05095"
     CDS             complement(1304..3040)
                     /locus_tag="PLF20_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80413.1"
                     /translation="MKKLKTIYFGNSCIALLLIVLTIACTQQKPAPLLQKSIVRIEQM
                     PDLPQPLSIIDWGEKARQFDSLVFDFEATTPFAPYIWWDTNHRNFPQNTYGIYTAIGD
                     ARQGSSKNGGEFHEALVTLPALLSAGLIGIDKTNQNGYNYVELAQNYFNRDNGWNIVM
                     NNTHPDMAHLGGGYGRDWWYDVYPNVLYYSVAALFPHVQRNDEIMRSVANKFYQADSI
                     LNGCYDYSYFDYGRMKPMRNNIPFQQDAAGGHAWVLYSAYKKFGDTKYLQGAISACKA
                     LDNQKESRFYEILLPFGAYTAARLNAEQGQNFDVEKILNHIFDGCQSTDGRYGWGVIA
                     EKWGDYDVYGLQGSITDGGGYAFLMNSVVMAWPLLPMVKYDPQYARAIAKYLLNAVNA
                     ARLFYPDQMDEQHQWLSNKKDITKGIIAYEGLRKHDDYGKPELKGITPVALGDGPKWA
                     PGQPEESMFSLYSSAVAGIYGAIVNKTDIEGILRLNCNATDFYGDNDFPVYLIYNPYL
                     ENREINYSIDTASDLFDILSRNYICRNGEGAVKLEIPAHTALLIAELPSMSKIKFSNH
                     KIWCNDKIVSWK"
     gene            complement(3037..4287)
                     /locus_tag="PLF20_05100"
     CDS             complement(3037..4287)
                     /locus_tag="PLF20_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX80414.1"
                     /translation="MLKKIKIEYEHFLSMPYNMRVLLITNMLYSLVLPVVEIFLAAYI
                     MRFYNETIYVALFQLAMYTGIILTSITNGFLLKHFKIAHLYSFGILLSGLVMASMMQI
                     EHLSVAQLFLAGLLIGAASGFFWTNRYLMTLNSTMDTNRNYFFGLESFFFTLSNIIVP
                     LIIGAILAGIDGMRIGSITFSIQTGYQIVTLIVFAITILACISLARGHYTNPAQKEFF
                     YLKFHNLWNLQIVLAALKGLVQGFLVTAPAMLIMRYVGKEGSLGLIQGISGGLTALIV
                     YLLGRFTQPKHRLAVFSVGLSVFLLGTIVNGILLSATGVIVFILCKVVFQPLHDLAYY
                     PTMMKVIDVVSVKENRNPYAYILNHEFGLYIGRVIGLGIFILLALMVSEVFALRWSLI
                     IVAAIQMLSIPLAKTIMKKSYQPL"
     gene            complement(4302..5339)
                     /locus_tag="PLF20_05105"
     CDS             complement(4302..5339)
                     /locus_tag="PLF20_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008775385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HOX80415.1"
                     /translation="MVKRFKNNPLLCPSDIEPSMPDMEVACLLNPGVFRYEDKIWLVL
                     RVAERPKQEEGKISFPVYNDKGKIEILSFQKDDTDLDAHDSRVIRYKGSDYLTTLSHL
                     RLVCSEDGIHFMEPEGYSSIMGQGPLESFGIEDCRVATFDDFYALTFTEVSRYGVGVG
                     LIITQNWKDYQREGMIFPPHNKDSAIFEQKIDGIYYAFHRPSSPELGGNYLWIAESPD
                     LIHWGKHRCIATTRERMWDSARIGAGASPILTPEGWLEIYHGANEKNEYCLGVILLDK
                     KYPWKVLARSQEPIMQPEMEYELKGFFGHVIFTNGHLVEGDTIKMYYGASDEVICGAT
                     LSISEIMNTLK"
     gene            complement(5646..6401)
                     /locus_tag="PLF20_05110"
     CDS             complement(5646..6401)
                     /locus_tag="PLF20_05110"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOX80416.1"
                     /translation="MKKVLLFLFLVALWSISVKAQEIFIEGHIVNLNHQPVSYAHVIL
                     LRNLTGTVSDSAGRFMMPLKYGDTLIISHVGYATRVYSLNESSLLYDHRLEIVLLDKI
                     YELKEVVITTFPKTWEEFKYAFENLKGDEEEQPADLQLNKEIPLGYNIPQSGFGITMN
                     GPITALYNIFSHEGKSRRKLARLLENDKRNRAVSQRYNYDLIKKLTGITQQETIERFM
                     QFCHLSDEFVLQSSDYELYLAILECYREFCEES"
     gene            complement(6494..8899)
                     /locus_tag="PLF20_05115"
     CDS             complement(6494..8899)
                     /locus_tag="PLF20_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX80417.1"
                     /translation="MFKKTFIFIFMALVPYVAMSQYMLSGTVCDASSGQSLVGANVVI
                     LNSPYGAVTNDVGYFSIKKLPSGNYPLKISYVGYESYFDTIKLDRNLFLKIKLQTLAH
                     MQEEVIVKSTRVDISVPTTFSELHRKEIRKLNIGRDLPYVLQMSPSVVASSDAGTGIG
                     YTSLRIRGTDMTRINVMVNGIPLNDPESQQVYWVDLPDFASSTDNIQIQRGVGTSVNG
                     AGAFGASINLLSTKISDEPQTIIHASGGSFNTLRNYISFSTGKSSSGFALDGRLSRIT
                     SDGYIDRASANLKSFFLSGGYYGKKSLLRINIMGGKEITYQAWEGVPSELLDKWRTFN
                     PAGLYTNEYGDTLYYKNQIDDYQQDHYQLLYSLSPSSNITLNLAAHYTHGFGYYESFR
                     EDAKYSKYALPDLIIGNDTIRKTDLINRKHLDNDFAGTTFSFIYEKNPRWRFTLGGAY
                     NYYKGQHFGRIIWARYASASFPENDYYRNEGIKKDFNIYGKASWEMTKYLSWFADMQY
                     RRVDYKIEGIHDDQRRLDQSHLFNFFNPKAGMNISFNERQRLYLSLAVANREPSRDNY
                     RDADEGYTPQPERLYDLELGYEWSDTHLQFGTNFYWMQYKNQLVLTGKINNVGDPIMM
                     NVPDSYRAGIELQGNAQLAPSLHLIANLTLSRNKIKNLTEYVDNWDTGDQQSIYYKET
                     DISFSPPVIAGLSIQYMPLQSLTFMLQSKYVGKQYIDNSSSNERKLHPYFVSDLRIDY
                     SLSIPYIRNMNLFFVVNNIFDHKYESNAWVYSYIYENQRRKMDGYFPQAGIHFIAGLE
                     ITL"
     regulatory      complement(8953..9051)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9135..9554
                     /locus_tag="PLF20_05120"
     CDS             9135..9554
                     /locus_tag="PLF20_05120"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HOX80418.1"
                     /translation="MTIAIINGPNLNLLGKRQPHIYGSERFEDYLRQLETTYPYINFI
                     YRQHNMEGEIINTLHEFGFSTDGIILNPGGFTHTSVAIADAIRSIYCPVVEVHISMLF
                     GREPYRQKSLTASACKGFICGFGLESYRLAVESFLQK"
     gene            10141..10551
                     /locus_tag="PLF20_05125"
     CDS             10141..10551
                     /locus_tag="PLF20_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80419.1"
                     /translation="MNKIKFLLVAVCCITATLAFKDASAQITVGPVNFNYYISDPEAP
                     PTTEYSVYAVLETSEGNLYPIPIGEVTGQGLKYLPQVINFPGVVPPNPMPRYPYRIIV
                     MVYRGSIVRTGVSDWANEEGLYPCTPNPIKVQQF"
     gene            10607..12256
                     /locus_tag="PLF20_05130"
     CDS             10607..12256
                     /locus_tag="PLF20_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOX80420.1"
                     /translation="MEPHYKKSLLLVLAVLLFRLNLFSQKELDIWYFGNKAGISFVNG
                     GPIALLNNNEFIPGYSSTISDSSGNLLFYGQIRLLNRNHTLMPNGDLGTTGEHGMIGI
                     QTKIPDTIYYIFHVDGFGFPNPSVHEGLTYSILNMNLDNGLGDIEPSKKSIPLCGASQ
                     AFGCITATRHSNNHDAWIISRIHDPDSNFYVSYLLNDNGVVDQPVFSNSGVFLDYSIS
                     TMIQALKISQDGKHLVCCYSDRFEVCSFNTATGEIQPKFTIIPSYIPVGPCFPDDLAF
                     SVDGKFVYVACHYYCTAISGCAAVFQYNMERGDSLSFKDSEKFIAIGSLMGIHLGPDN
                     KIYATQTFTDSLSVIHHPSLPGSACQWQKNYLSLRGRQSGVGLSQGLQRYFAYINHSG
                     QCQGMPVSFSPNTYPAPDSLWWNFGDPASGSANTSIDSTPQHIYANPGSYTIQMIVKH
                     IDNRYDTAIFHLFIEPQPIPNLGPDITVCENQAVILDAGYNPEWTYLWNTGATSSSIT
                     VDTSGTYSVTVSSQSGCSGSDSINVFIGQGTKPPPRLIKHD"
BASE COUNT         3697 a         2592 c         2369 g         3761 t
ORIGIN      
        1 taaccatcgc ctaagaaact gtcgtaaatc tgatctccga aatattcggc tggacgatct
       61 acccatctac catcgggaaa cttgatcggg atggccggat tacggaaaaa ggcatagcgt
      121 ataacgcttc caccattgcc accgtagcca tctcccgaac ttccaatgat atcggttttc
      181 gaatgcccaa ctatcatgct catgccagtt tttaaccatg ggaaaatctc cgtctcgaga
      241 ctcaaatgcg atgaaactct tgtatatcca gtattttcga tgactcctct ctggtcgaag
      301 aagttacccg acataaaata gctcgtttta cctgtggatc ccgaatagct gagttcgtga
      361 gagttgatag gagcaatccg aaaaagttct tccagataat tgatatcgac aaaattggct
      421 gcaagttgtg gcgtaatcaa gacacgctgg agaccttcgg gcaaatattt attgtcgttg
      481 gtcgcagctt cattataaat ggtgatataa tccgctgtat tgaccatttt cggaatatgc
      541 cctacggcct gaactccact ttgaccatga tactgaagca caggtttgcc ttctttgcct
      601 tttttggtag taatcaatat gataccatta ttggctcgag ctccataaat agctgccgaa
      661 gaggcatcct tgagtatggt gatgttttcg atatcgccgg gtgcaatcac attcaaagca
      721 tcggcagtag gaatgccatc cacaatgtaa agtggatcgt tactcgagtt gatggaacct
      781 atacctcgaa ttcttaccga aacaccttcg cctggtgctc cggtattttg agtaacctgt
      841 acacctgcgg cagctccctg taaggcctga tcgatacccg ctacaggtaa acgggtaagc
      901 tcttgagtcc ctaccttcga gattgctccc agaacattac gtttcttggt tgtgccgtaa
      961 ccaatcacta ccagttcctc gagcatcacc tgcgaaggtt ccagcttaat atcgagaacc
     1021 ttctgattct ggaagacaac ctcctgagtg ttgtatccaa caaaagagaa aacaatcact
     1081 gcttgaggct tcagagcggc cgattccaaa acgtatttac cgttaacatc cgatactgtg
     1141 ccaattgtgg ttcctttgat gtagatggtt acacccggta aggcctcacc acttttggca
     1201 tccgtcactt tcccttctat acggttttgg gccagaataa aagaagaagt tgttaacaaa
     1261 atgaccatga aaagtaatag tcgtttcata agaatagatt tttttatttc cagctaacaa
     1321 ttttgtcgtt acaccatatt ttatgatttg agaatttaat cttcgacata gaaggcaact
     1381 ctgcaatcaa aagagcagtg tgtgcgggaa tttctaattt gacagcaccc tcgccatttc
     1441 tgcaaatata atttcgagag agaatatcaa aaagatcaga agcagtatct attgaataat
     1501 ttatttcacg attttcaaga tatgggttat agatcagata aaccgggaaa tcattgtctc
     1561 cataaaagtc ggtagcattg cagttaagac gtaatatacc ttctatgtcg gttttgttga
     1621 cgatagcccc ataaattcca gccacagccg aactatagag gctaaacatg gattcttcgg
     1681 gttgacctgg tgcccatttg ggcccatctc caagggctac gggagtaatt cccttgagtt
     1741 caggtttccc atagtcatcg tgcttgcgta gcccttcata agcgatgatt cctttagtaa
     1801 tgtctttttt attgctgagc cattgatgtt gctcatccat ctgatcggga taaaaaagac
     1861 gagcagcatt gactgcattg agcagatatt ttgctatagc ccttgcgtac tgaggatcgt
     1921 attttaccat aggaagcaga ggccatgcca tgaccacaga attcatcaaa aaagcatacc
     1981 ctcctccatc ggtgatactg ccctgaagtc catatacatc gtaatcgccc catttttcag
     2041 caattacacc ccatccatat cttccatcgg tagattggca gccatcgaaa atatgattga
     2101 gaattttttc gacatcgaaa ttttgacctt gttctgcatt gagacgagcg gccgtgtaag
     2161 ctccaaatgg aagaaggatt tcgtaaaacc tgctttcttt ctgattatcg agagctttgc
     2221 atgcacttat ggcaccttga agatatttcg tatctccgaa tttcttgtaa gccgaataga
     2281 gtacccatgc atggccaccg gctgcatcct gctgaaatgg gatattgtta cgcatgggtt
     2341 tcatccttcc ataatcaaag taggaataat cgtaacatcc gttcaaaatg ctatcggcct
     2401 gataaaattt attggccaca cttctcatta tctcgtcgtt tctctgaaca tgagggaaaa
     2461 gtgcagcaac gctataatac aagacatttg ggtaaacatc gtaccaccaa tccctgccat
     2521 agcctccacc tagatgagcc atgtcgggat gggtgttgtt catcactatg ttccatccgt
     2581 tatcgcgatt gaagtaattt tgcgccaatt caacgtaatt atagccattc tgattggttt
     2641 tgtcgattcc aataaggccg gcactcaata aggctggaag tgtaacaagt gcttcgtgaa
     2701 actcacctcc atttttcgat gagccctgac gagcatctcc tatagccgta tatattccgt
     2761 aagtattctg aggaaaattc cgatggttcg tatcccacca gatataagga gcaaacggag
     2821 tggtagcctc gaaatcgaag acaagactat cgaattgtct ggctttttct ccccaatcga
     2881 taatggaaag aggttgtggc aaatcgggca tctgttcaat tcttacaatg cttttttgga
     2941 gcaaaggtgc aggcttctgc tgtgtacaag caatggtcag aactatgagc aacagagcga
     3001 tacaggaatt tccaaaataa atcgttttta atttcttcat aggggttgat aagatttttt
     3061 catgattgtt ttagccagag gaatagacaa catttgaatg gcagctacaa taatgagtga
     3121 ccatcttaag gcaaatactt ccgaaaccat gagagccagc aggatgaata tccccaaacc
     3181 tatgactctg ccaatgtaca gcccaaattc atgattcaga atgtaagcat aaggattacg
     3241 gttttcttta accgaaacca catcgattac tttcatcatc gtgggataat aagccagatc
     3301 gtgaagaggc tgaaaaacaa ccttacaaag tataaacacg attacaccag tggctgatag
     3361 cagaattcca ttgacgatgg ttcccagcaa gaaaacgctt aatcccaccg aaaaaaccgc
     3421 cagacgatgt ttgggttgag taaaccgtcc taaaagatac acaatcaacg cagtgagccc
     3481 tccgctaatc ccctgaatca atcccaacga cccttctttc ccaacataac gcatgatgag
     3541 catggctggt gcagtaacta aaaaaccctg caccaatccc ttcaatgctg ccagcacaat
     3601 ttgtaaattc cacaaattat gaaatttcaa atagaagaat tctttctggg ctggatttgt
     3661 ataatggccc ctcgccaagg atatacaagc caaaatagtt attgcaaaaa caattaaggt
     3721 aacgatttga tatccggttt gaatgctaaa ggttatgctt cctatcctca ttccatcgat
     3781 accggccaat atagcgccaa taatcaaggg aacgataatg ttcgagaggg taaagaaaaa
     3841 cgactccaat ccaaagaaat aattccggtt ggtatccatg gtagagttca atgtcatcag
     3901 atagcgatta gtccagaaga aacccgaagc tgctccaatg aggagacctg ccagaaaaag
     3961 ctgggccaca ctcagatgtt cgatttgcat catgctagcc atcacgagcc cgctcagcag
     4021 aataccgaat gaatagagat gagcaatctt aaaatgtttc agcaaaaaac cattcgtgat
     4081 ggaggtgaga atgatacctg tgtacatagc taattgaaac agggctacat aaatggtttc
     4141 gttgtaaaaa cgcataatgt aggctgccag aaaaatttcg actacgggaa gcacgaggga
     4201 atataacatg ttggttatca acaaaaccct catgttgtat ggcatcgaaa gaaaatgctc
     4261 gtattctatt ttgatcttct tcagcatatt ttcgaatatg attacttcag cgtattcatt
     4321 atttcagaga tagacaaagt agcgccacaa atcacttcat cactggcacc ataatacatc
     4381 ttgatggtat caccttccac cagatggccg ttggtaaaaa ttacatgacc aaagaaaccc
     4441 ttcagttcgt actccatttc aggctgcatg atgggttctt gacttctggc cagaactttc
     4501 catggatatt ttttatcgag aagaattact ccgagacagt attcattttt ttcgtttgca
     4561 ccatgataga tttcgagcca accttcagga gtgagtatgg gcgaagcgcc tgctccaata
     4621 cgagccgaat cccacattct ttcacgggtt gttgctatac atcgatgttt tccccaatga
     4681 atcaaatcgg gagactcggc aatccaaaga taatttcctc ctaactcagg actactgggg
     4741 cgatgaaaag cataatatat tccgtcgatt ttttgttcga aaatcgcact gtccttgtta
     4801 tgaggtggaa aaatcatccc ttcacgctga taatccttcc agttttgagt aattatcaaa
     4861 cccacaccta caccatatcg cgaaacctcg gtaaaagtca acgcatagaa atcatcgaaa
     4921 gtagctaccc ggcaatcttc tattccaaac gattctaaag gcccttgtcc catgatggaa
     4981 gaataacctt caggttccat aaaatgtatc ccatcctccg aacaaaccag tcgtaaatgc
     5041 gacagcgttg taagataatc gctgcctttg taccgtatta cccttgaatc atgggcatcc
     5101 agatcggtat catctttttg gaacgaaaga atctcgattt tacctttatc gttatagaca
     5161 ggaaaagaaa tcttaccttc ttcctgtttt ggacgttccg caacccttag taccaaccat
     5221 atcttatctt catatctaaa tacgccggga ttcaataaac aggcaacctc catgtccggc
     5281 atagatggct cgatatctga aggacaaaga agtggattat ttttaaatcg tttcaccatg
     5341 gcttgatttg ttttgctcaa aattactgat actttgtgcc agagcgtgtc cgctcagtaa
     5401 catattttgt ccgttatgtt atgaatttta ttaggaaggt ggcaagattt ccagggtatg
     5461 tgttgcttgg ggatacagag attgtgggat gaaaggactc ctattaacaa gcaactaatt
     5521 catttttagc ttattgactt ttaactttcg gctctctata ccccatctac catcctctgt
     5581 cctgtctatt cttaaattag gttggccatt ttacaaattc tggtgtagaa tcgagtgcca
     5641 ctgccctaac tttcctcaca aaattcacga tagcattcca aaatggccag atacagctcg
     5701 taatcggaag attgcagaac aaattcatcg gatagatggc aaaactgcat gaatctttct
     5761 atggtttctt gctgggtaat acccgtgagt ttttttatca aatcatagtt atatcgctgg
     5821 ctaacggctc tattacgctt gtcgttttcc agtaaacggg ctaacttcct cctgcttttg
     5881 ccttcatggc taaaaatatt gtacaatgca gtaatcggac cattcattgt aattccaaaa
     5941 ccactttggg gaatattata tcccaagggt atttccttat taagttgcaa atcagcaggt
     6001 tgctcctctt catcgccttt caggttctca aaggcatatt tgaattcttc ccatgttttt
     6061 ggaaacgtag taataaccac ttctttcagt tcatatatct tgtccaacag aacgatttca
     6121 agcctatgat cgtaaagtaa agaagattca ttcaaggaat aaactcgggt tgcatatcct
     6181 acatgactga taatgagtgt gtcaccatat tttaacggca tcatgaaacg ccctgccgaa
     6241 tcgcttaccg ttccagttaa gttgcgtaat aaaataacat gcgcataaga aacaggttga
     6301 tgattgaggt tgacaatatg tccctcgatg aaaatttctt gggcttttac agagatactc
     6361 cataaagcca ctaaaaataa aaaaagcagc acctttttca tgatgctgcg aaattataac
     6421 ttaatgaggt acttacatat attaaaaggt taaaaagtgt taattttcaa aaaattcgtc
     6481 attatttatt tattcaaaga gttatttcga gtccggcaat gaaatgaatc cctgcctgag
     6541 gaaaatagcc atccattttt ctgcgttgat tttcataaat atagctataa acccatgcat
     6601 tgctttcata cttatgatcg aatatgttgt ttactacaaa gaaaaggttc atgttgcgta
     6661 tataaggtat tgaaagtgaa taatctatcc gcaaatcgct tacaaaataa ggatggagtt
     6721 tcctttcgtt gctcgagctg ttatcgatat attgtttacc cacatatttc gactgcaaca
     6781 tgaacgttag gctttgaaga ggcatgtact gaatacttaa gcctgcaatc actggaggtg
     6841 aaaatgaaat atcggtttcc ttgtaataaa tgctctgttg gtcacctgta tcccaattat
     6901 cgacatattc cgtcagattt ttaatcttgt tgcggctaag ggttaagttg gctatcaaat
     6961 gcaaagaagg agcaagctga gcattcccct gaagctctat tcctgctctg taactatctg
     7021 gaacattcat cattatcggg tctcccacat tattgatttt gcctgtcaat accaattggt
     7081 ttttgtattg catccaataa aaatttgttc caaattgaag atgagtgtcc gaccattcat
     7141 agcctaattc gaggtcatac agacgttcgg gttgaggtgt ataaccttcg tcggcatccc
     7201 tgtaattatc acgcgaaggt tcacgatttg ctactgccag tgaaagataa agccgttgtc
     7261 tttcattgaa gcttatattc attccagcct tgggattgaa aaaattgaat aaatgacttt
     7321 gatcgagcct tcgctgatca tcgtgtatac cttctatctt gtaatcaaca cgtctatatt
     7381 gcatatcggc aaaccacgaa agatatttcg tcatttccca tgatgctttg ccgtagatat
     7441 tgaaatcctt ttttattccc tcattgcggt aataatcgtt ttcggggaag ctagccgaag
     7501 catacctcgc ccaaatgatt cttccaaagt gctgaccttt gtaataatta taggctccac
     7561 ccaaagtgaa tctccatcga ggattttttt cataaatgaa agaaaaagta gttcctgcaa
     7621 agtcgttatc gagatgtttc ctattgatta aatcggtttt acggatagta tcgtttccta
     7681 taatgaggtc gggcagggca tatttactat atttagcatc ttctctgaag gattcgtaat
     7741 atccaaaacc gtgtgtataa tgggctgcta aattgagggt aatattactg gaaggggaga
     7801 ggctgtataa gagctgataa tggtcttgct gatagtcgtc gatttggttt ttataataaa
     7861 gcgtatcacc atactcatta gtatagaggc cggctggatt aaaagtacgc catttgtcga
     7921 gtaattctga aggaacacct tcccaggctt ggtaagtaat ctcttttcca cccatgatat
     7981 tgatgcgtaa cagacttttc ttgccatagt agcctcctga tagaaagaaa gacttcaggt
     8041 tagcgctggc tctatcaata tagccatcgg aggttattcg gctaaggcgt ccatcaagcg
     8101 caaagccact ggaagatttt cctgtgctaa aacttatata atttcgtaag gtattgaaac
     8161 tgccccctga agcatgtata atggtttgag gttcatcgga tatcttggtg ctcaataaat
     8221 tgatgcttgc gccaaaggct cctgctccgt tcaccgacgt ccctactcct ctttgtattt
     8281 gtatattatc ggtcgaagat gcaaaatcag gtaaatcaac ccagtaaact tgctgagatt
     8341 ctggatcgtt cagtggaatt ccatttacca ttacatttat gcgagtcata tctgtgcctc
     8401 gaattcttaa actggtatat ccaatccctg tacctgcatc ggaacttgct accactgaag
     8461 ggctcatttg caacacataa ggtaaatctc gtccaatgtt caatttcctt atctccttcc
     8521 gatgtagttc gctaaaagta gtgggaaccg aaatatctac cctggtggat tttacgatta
     8581 cttcttcctg catgtgagct aatgtctgta attttatttt taaaaagaga ttcctgtcta
     8641 atttaatggt atcaaaatat gactcatatc ctacataact gatttttaaa ggataattac
     8701 ccgaaggaag ttttttgatg gaaaaatatc cgacgtcgtt ggttacagct ccataaggag
     8761 aattcaatat aacaacattg gctccaacga gactttgccc gctcgaggcg tcacaaaccg
     8821 tcccactcag catatactga gacatggcca cataggggac aagcgccatg aaaatgaaaa
     8881 taaaagtttt tttgaacata aataatggtt ttaattgtgg aacaatgtat taaaaccata
     8941 taggggaaaa ggtcatgaat gaattatatc tccctacgcc ggtataatcc ggatcaggtt
     9001 caaagggtat gatctcagcc ccgctatatc gggacacccc tataaggtat ctacggcaaa
     9061 attagaatat tttatctaat aaacgatttg ataaaataat ttgctatttt tgatgaaaaa
     9121 tgatttgata aaagatgact atcgcgatta tcaatggacc aaatctaaac cttttaggaa
     9181 aacgacaacc tcatatctat ggttctgaac gattcgaaga ctatctccgt cagctcgaaa
     9241 caacttatcc ttatatcaat ttcatctatc gacaacataa tatggagggt gaaatcatca
     9301 ataccttaca tgaatttgga ttttccaccg atgggattat actaaaccct ggcggtttta
     9361 cacatacttc ggtagcaata gcagatgcta tacgttctat ttactgccct gtggtagaag
     9421 tgcacatcag catgctcttt ggccgtgaac cttaccgaca aaaatcgctt actgcttcgg
     9481 cctgcaaagg ttttatttgt ggctttggat tagaaagcta tcggttagct gtagaaagct
     9541 ttctccaaaa ataaaaataa acatctctct aatagagaat taccacagca ctttttgttt
     9601 ttctgaaaat ggttagaatg agccaaatca ttattattga caagagctat tttaaatgaa
     9661 taaattttat agtcaatgtt tcttgattca aaagaagtaa gaaatatttg gaaaaacgcc
     9721 caaaaagtag aaaaagcttg tatatttgtg ttatgaattc cttattttga ttcttagaaa
     9781 aagcatctat gcccatgggg caagtctctg atttgtttac acgggctttc cttcagagag
     9841 aagcaattaa ggaatgctac tatttcaaat agaaacacag gttttaataa agtttaactg
     9901 aattggaaac tcttctcatg tatagcgtaa aagttaatag ctatcctttc atcaaatggt
     9961 ttaattcttt tgatgataag gttgccagat tattattatg gctactgcat ccaatataac
    10021 aaaacccgaa cggtgttgtg cgtcagctaa tgaggtacag aaagatactt tcttatcgtt
    10081 ttacaaatta acataataca ttaatttaat taacttatta aaatttaaat ttataacaaa
    10141 atgaacaaaa taaaattttt attagtagca gtttgttgca tcacagctac tttagctttt
    10201 aaggatgctt cagcacagat aacggtaggt ccagttaatt ttaattatta catatctgat
    10261 cctgaggccc ctccaactac agaatattct gtttatgcag tccttgagac aagtgaggga
    10321 aatctctatc ctatccctat aggagaagtt accggccaag ggctaaaata tttaccacaa
    10381 gtcattaatt ttcccggggt tgttccgccc aatcctatgc caaggtatcc ttatcgaata
    10441 attgttatgg tgtacagagg cagcattgtt cgtacaggag tttccgactg ggccaacgaa
    10501 gaaggcttat acccctgtac gcctaatcct atcaaagtcc agcagttttg attaaatttg
    10561 catttccccg gcctgttccg gggaaatgct ttatcttaat tgaaaaatgg agcctcacta
    10621 taaaaaatcc ttacttttag tcttagccgt tctattgttc cgattaaacc tattttccca
    10681 aaaagaactc gacatttggt attttggaaa caaagccggc attagtttcg ttaatggagg
    10741 acccatcgca ttattgaata ataatgaatt tattcctgga tattcatcta ctatatctga
    10801 ttcctcagga aatttacttt tttatggtca aattcgttta ctaaaccgca atcacacctt
    10861 aatgcctaat ggtgatttgg gaactacggg ggagcatggt atgattggta ttcagacgaa
    10921 aatcccagat acgatttatt atatttttca tgtggatggt tttggatttc ccaatccatc
    10981 agttcacgaa ggcttgacct attccatatt gaacatgaat ctcgacaatg gactgggcga
    11041 tattgaaccc agcaaaaaaa gtattccttt atgtggtgca tcacaggctt ttggatgtat
    11101 tacggctact cgccacagca acaatcatga tgcctggata atttcgagaa tacatgatcc
    11161 tgattcgaat ttttatgtat cctatctttt gaatgataat ggtgttgtcg atcagcctgt
    11221 atttagcaat agcggggtat ttctggatta ttctatatct acaatgatac aggccttgaa
    11281 aatatcccag gatgggaagc atctggtatg ctgctattcc gacaggtttg aggtgtgcag
    11341 tttcaatact gccacgggtg aaattcagcc caagtttacc attattccca gttatattcc
    11401 ggttggtcca tgctttcctg atgatttggc cttttcggtt gatggcaagt ttgtgtatgt
    11461 tgcatgtcat tattactgta ctgccatttc aggttgtgct gctgtatttc agtacaacat
    11521 ggagagggga gattctctgt catttaaaga ttctgaaaaa ttcatagcca ttgggtcatt
    11581 aatgggcata catcttggtc cagataataa gatatatgct acacagacgt ttacagattc
    11641 tcttagtgtt attcatcatc cctctttacc tggttcagcc tgtcagtggc aaaaaaatta
    11701 tctcagcctg cggggaagac aatcaggtgt agggctttca caaggtcttc aacgttactt
    11761 tgcctatatt aatcactctg ggcaatgtca aggaatgccg gtaagctttt cacccaacac
    11821 ctatccagca cccgattcgc tttggtggaa ctttggcgat cccgccagtg gctctgccaa
    11881 tacttctata gatagcactc ctcaacatat ttacgccaac cctggttcct acaccataca
    11941 aatgatagta aaacatattg acaatcgcta cgacacggcc atatttcatc ttttcattga
    12001 gcctcagccc atccccaacc ttgggccgga tattacagta tgtgaaaacc aggcagtaat
    12061 tctggatgca ggctacaacc ccgaatggac atatttatgg aatacaggag ctacatcgag
    12121 cagcataact gtcgacacca gtggaacata cagtgttacc gtaagttctc aaagcggctg
    12181 ttcgggcagc gatagcatca atgtatttat tggtcaaggc acaaaacctc caccccgtct
    12241 catcaaacac gattgattat aaaccaatag aaaaaatttt gtaggctatt caagcaaaaa
    12301 cgaatcgaat ttttttaaac tctatcgaca tcaaattaaa tttatttatg aatattatat
    12361 taaatagagt ctgtccatac agtcatcaaa attttcagta aaagtataaa cttcttata
//
LOCUS       DAOORU010000057        12267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_6387_length_12267_cov_6.157141, whole genome shotgun sequence.
ACCESSION   DAOORU010000057 DAOORU010000000
VERSION     DAOORU010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12267
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6387_length_12267_cov_6.157141"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..3248)
                     /locus_tag="PLF20_05135"
     CDS             complement(303..3248)
                     /locus_tag="PLF20_05135"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HOX80421.1"
                     /translation="MKTSRFFVVWLIPIMFMIMGCHREFSSGEEPARWVDPFIGTAGH
                     GHTYPGATLPFGMVQLSPQTRLEGWDGCSGYHYSDSILYGFAHTALSGTGCSDYGDIL
                     LMPVVGKPVFSNSEYSSPFLKEKESACPGYYKVFLEKPGVWAELTATPRVGYHRYTFP
                     PTPQAQIIIDLQHRDKVVDSWIEFVGDRQIRGLRRSSNWAQDMIWYFYMEFSRPFVRK
                     GIAINDSLHEGILKAEGTNIKAYVGFDTQDTSVIEVKVALSPVDAEGAYNNLKNELPA
                     WGFEETKNQAYEIWNKELAKIKMKGGTKNQLTIFYTALYHAMLQPNIFMDVDRRYRGI
                     DRKVDTADDFDNYTVFSLWDTYRAWHPLMTIIDSGRTVNYIRTMLNHFDKSGALPVWE
                     LAGNETWCMIGNHSIPVIADAWVKGIRGFDPDRALEAMIRSVNSDRFGMDIYRRMGCL
                     LGDKEHESVSKTLEYAYDDWCIAQIAKDLGNDTVYSEFIKRAQFYKNLYDPSTGFIRP
                     KINGGWLTPFDATKVDWNYTEANAWQYNFHIPQDVNGHIALMGSDVAYLTKLDELFNT
                     SQKVSGRDMKDISGLIGQYAHGNEPSHHVAYLFSYAGQPWKTQEKVRQIMDDFYTNAP
                     DGLIGNEDCGQMSAWLILSAMGFYPVTPGSDIYVLGTPWFKEMEVQFENGKTFTITTD
                     HPSPKSFYVQKVLLNGQSYDKSFIRHQDIKSGSHLHFKMGKHPNETWGNPPESRPKSI
                     IQDHLLLPMPYINSPDKRIRRSIQISMGAPIAGSEIYYTLDGTMPDKTSLVYSQPIQL
                     TESAQVIAIALHPEMGFSYPAMAEFVKIDTNLKIRLLSNYHPNYSAGGPDALIDGLRG
                     PDNWRLGGWQGYQGTDFEAIVDMGSKKTIHHCALGCLQDAGSWIWMPSKVEFFLSDDG
                     KNFQKIGEVAPNIATDDYRKQVKDLGITINRSGRYIKVRAINLGTIPSWHPGAGDKTF
                     IFVDEIVVK"
     gene            complement(3235..5802)
                     /locus_tag="PLF20_05140"
     CDS             complement(3235..5802)
                     /locus_tag="PLF20_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80422.1"
                     /translation="MDSIFLKRYFFFLTLQVLWGLSCHASGFESKKTDLCNLKWEFSK
                     TNSSARYLARVPGTIHTDLYRDGLIPDPFFACNEKELQWIGQTDWSYHTHFYLNENDF
                     SFQNLELVFEGLDCFAEVILNGHNILEANNMFREWRVDIEPFVEIGGNFLEVRFFSAL
                     NRFLADSAALGYPLPGGRWNLSRKAAYHFGWDWGPTFITCGIWKPVYLVAYNGIKVED
                     ASITTQSIVHDTALLKCEITVFSHFDKEISTKFELYFHGNLISNKDLNIEKGSHSFEF
                     DFPVVHPQLWWCNGLGKPYLYEFELSLKTEKKEWFHKNIRVGIRNIQLVLQPDSIGTS
                     FYFKLNGVPVFMKGANYIPSHSFLTETTNSDYERLLRNAKEANMNMLRVWGGGVYERD
                     EFYSLADEMGILIWQDFMFACAMYPGDEEFFQNVRQEAIEQVRRLRNHPCLALWCGNN
                     EIDEAWHNWGWRNQYQNSKLRDAVWSHYLNLFHRLLPAVVHQYDPGTSYIPSSPRFGW
                     GRSQSMVSGDSHYWGVWWGQQPFEILLQKIPRFMSEFGFQSMPEISTIRKVQPPEADS
                     LFSPQLRCHQKCSLGFEILSVYLEREGFSPQTLEEYIYTTQLVQAKGIGLGLAAQRAS
                     RPHCMGSLFWQLNDCWPVVSWSSMDFQGNRKALFYKTRQLFDPIAIETFIHEQEVKIF
                     LFSDLPKDIKGTLTFEMRYYTGKLVPILQAEVNVSANSSSCVYSVPLNEFQQVGDYLF
                     VATLRFDDSIIRKAYCLPGLLSQQNLPEAHLLTSIRRENDGYIISVKSDVFIAFIQFY
                     LKESPAEFSDNFIHVWPGQNVEIFCRTSLPEQEFQRQFSFFTLNELYRINLNRHENK"
     gene            complement(5924..7117)
                     /locus_tag="PLF20_05145"
     CDS             complement(5924..7117)
                     /locus_tag="PLF20_05145"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX80423.1"
                     /translation="MLKNKTLPVFMAFLAMGFGDVVGPLVSLVKNSFEVSNFVASLMT
                     TSGFIMFGLLSLPLGIIQDKKGKKFVLSRGLLVAFIGLMLPILFGMYGKTPQMQDSGQ
                     LKLIMLFVSIFLLGAGATMLQVSGNPIMRDVSPQGRYSSNLSLAQSIKAIGSSLGFLL
                     PPAVSIWFGLDWTLLFPVYAFIVLITWLWINAVPIEECRYESASSANFSSTFSLLKNP
                     FVALMVLGIFLYVGAEVSMSAQIPILMDKVYGIKTLGLWLAWALFFLPIFLGRLLGSF
                     ILRILSNRRFLLITALLSIAGMTMLFFGSKSVAFCGIFLVGLGFANIFPLIFSITVDA
                     IPQRANELSGLMVMAIVGGALIPPITGAVADINVMLGFIVPAACILYVLILAISRPTP
                     HPYVN"
     gene            complement(7126..8157)
                     /locus_tag="PLF20_05150"
     CDS             complement(7126..8157)
                     /locus_tag="PLF20_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOX80424.1"
                     /translation="MAKKISLAEIAKSLGVSKTLVSLVINNKADEHGISAETQQRVRE
                     KIKELNYHPDELAQGFRTGKTKTIGLIVSDISNLFYSRLARRIEDYAWLNGYTVVICS
                     TDEDISKELEQIRMLRSRKIDGLIVSSSQEDGSFFNKLADENYPHVLIDRIFTEMHSP
                     NVSVDNFGGARMAARHLISQGMRNFCVISITPEHISTINERLKGFESALNEMGIKIPE
                     EWHIRAPFHQIDQAVKEGIQRIYQSAHLPDAIFTLNNNLTSATLKYLRKLSVRIPEEV
                     ALIGFDDLVYFSYTHPSISAIEQPIDRIGEKSFELLLRQIQKEKILPHEQSIYLPVDI
                     IIRESSIKN"
     gene            complement(8322..10622)
                     /locus_tag="PLF20_05155"
     CDS             complement(8322..10622)
                     /locus_tag="PLF20_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 protein"
                     /protein_id="HOX80425.1"
                     /translation="MKKNCLLFFFTFTVSIAVFSQTVISIIPQPVKIEMKSGEFRFGP
                     ETKIMYMDESLMQLAQYARQQWKFWSGWDGEIQQIKHEKLEGNFVLQIIQPAEKELST
                     EGYGIEITSQNVFLRSNTQAGLIYAIQTLNQLMSANPSQVLPACIIIDYPRFRYRGLH
                     LDVARHFMPADFIYRLLDEMVFHKMNVFHWHLVDDQGWRLEIEKYPKLTSIGAWRPDM
                     EDLDWNARPLQCQPTQEMYGGFYTQDEVRKIVKYAADRNITIIPEIEMPAHVMSALAA
                     YPQFSCSGKNLGVPSGSVWPITHIYCAGNDSAFIFLRDVLTEVMEMFPSIYIHIGGDE
                     ADKSEWNKCPKCQQRMQTENLKNVDELQSYFVQCIEKFLNANGRIMIGWDEILEGGLA
                     PNVVVMSWRGEEGGIAAARLKHNAIMTPASHCYFDYYQGDPAVEPLSIGGYIPLKRVY
                     SYEPVPQELSLEESHYILGAQANTWTEYIRTPQHAEYMIFPRLAALAEVVWSPNNLRN
                     WEDFSRRLENQFKCYDKLGIYYARSAFQARIISQPDSVRRCVEISIETEAPYGEIHYT
                     TNGKDPTDSSPVYTGPFALCESATVKAGVFHGGKCMGAIIAKKFSLHKAFGAKMTFQI
                     PSASRYNGNLGDGVWGSTNYRDGNWKGFLGDDAIIIIDLGKPTKIRSIKVDALQASGS
                     WIFFPEKAICELSKDGKEWKSVGEKMNEHSWQEPVPAIQLFGFENLRENVRFVRLKMK
                     NRGTCPLGHAGEGQPAWIFISEVEIL"
     gene            complement(11375..12019)
                     /locus_tag="PLF20_05160"
     CDS             complement(11375..12019)
                     /locus_tag="PLF20_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80426.1"
                     /translation="MKCILSTIIACMMIIKCIGQEEIKNPVLNKSLNKYVIGFIPSKA
                     NNIFGIAIGPFGSEVICDKPYTKYSHGLNIQIPGQGILQTFYIFNSPFKQVYKNKIID
                     DEFIKKDTSFKRAIHNGLILSLLGTFTDQVNGVSISGWMSLGKNINGVSINLLWNLYY
                     EINGLSIGIFNSTLVMNGIQIGFINRTVKLKGVQVGLWNRNEKRSLPLINWNFK"
BASE COUNT         3716 a         2574 c         2430 g         3547 t
ORIGIN      
        1 accaatcact aaaaaatcag tatgtacacg catgatgata tttttcgcaa atttaatctg
       61 ttacgcttga atagggttta ttttttaata accagataca ttgaagtccg ctattcctcg
      121 ttgagcactg caaacttgat aactaacaac tgacagccta taaaaaacaa attataaatt
      181 acaaattcta atcaccaatc accatccacc ttgtctcctc gtctccacgt ctcctggttt
      241 ccttgtggca gtctcccagt ctcgttgtcc cttcgtctcc attcaccatc cactaattcc
      301 catcatttca cgacaatttc atccacaaaa atgaaggttt tgtcgcctgc gccggggtgc
      361 catgaaggaa tagttcctaa attgatggct cttaccttga tatagcgtcc gctgcgattt
      421 atagtgattc caagatcctt cacctgtttc cgataatcgt cggtggcaat atttggagca
      481 acttcgccaa tcttctggaa attcttccca tcatcagaaa gaaaaaactc gaccttcgag
      541 ggcatccaga tccatgaacc tgcgtcttgt aaacatccaa gggcacaatg atgtatggtt
      601 tttttgctgc ccatgtccac aatggcttcg aaatccgttc cttgataacc ttgccagccg
      661 cctaaacgcc agttatcggg tccgcgtaag ccatcaatca aggcatcagg cccgcctgca
      721 gaataattgg gatgataatt cgataacagc cgtattttta aattcgtatc tatttttacg
      781 aattcagcca tggctggata actgaatccc atttcgggat gcaaggcaat ggctattacc
      841 tgagccgatt cagtcagttg tataggttgg ctgtaaacca atgaggtctt atcaggcatt
      901 gttccatcga gggtataata aatttcactc cctgcgatgg gagctcccat acttatttga
      961 atgcttcggc ggattctttt gtcgggcgaa ttaatgtaag gcataggcaa aagaagatga
     1021 tcctgtatga ttgatttggg acggctttcg ggcggattac cccaggtttc gttgggatgt
     1081 tttcccattt tgaaatgtaa atggcttccg gattttatat cttggtgccg gataaaggat
     1141 ttatcgtagg actgaccatt caggagtact ttctgcacgt agaaagattt tggagaaggg
     1201 tgatcggttg taatggtgaa ggttttccca ttttcaaact ggacttccat ttccttgaac
     1261 caaggtgttc ccaatacata aatgtcgctg ccgggagtga cgggataaaa tcccatagca
     1321 ctcaatataa gccatgccga catttggccg caatcttcgt tgccaatcaa accatcggga
     1381 gcgttggtat agaaatcatc cataatttgt ctgacttttt cttgagtttt ccaaggttga
     1441 ccggcatagg aaaagagata ggccacgtga tggctgggtt cgttaccgtg ggcatattgt
     1501 ccgattaatc cactgatatc tttcatatcg cggcctgaaa ctttctggct tgtgttgaaa
     1561 agctcgtcca gtttggttag atatgccacg tcacttccca taagggcaat atggccattc
     1621 acatcttgag gaatgtgaaa attatattgc caggcattgg cttcggtata attccaatcc
     1681 actttcgtag cgtcgaaggg agtaagccaa ccaccgttta ttttaggtcg gatgaaacct
     1741 gtagaaggat catagagatt cttatagaac tgtgctcttt taataaattc agaatagacc
     1801 gtatcatttc ctaagtcttt tgctatttga gcaatacacc agtcatcgta agcatattca
     1861 agcgttttgg agacagattc atgttcttta tctcctagca ggcatcccat gcgacgatag
     1921 atatccatac caaaacgatc ggaattaacg cttcgaatca tggcttccaa ggcacgatca
     1981 gggtcgaagc ctctaatgcc ttttacccat gcatcagcaa ttaccggaat ggaatgattg
     2041 ccaatcatgc accatgtttc gttgcccgca agttcccaaa ccggcaaggc accgctttta
     2101 tcgaaatgat ttagcatagt tcgaatgtaa ttgaccgtgc ggcccgagtc tattatcgtc
     2161 atgagtggat gccatgccct gtatgtatcc caaagtgaaa ataccgtata attatcgaaa
     2221 tcgtcagccg tatcaacctt tcgatctatt ccacgataac gacgatcgac atccatgaag
     2281 atgttgggct gaagcatagc atggtaaagt gcagtataaa aaatcgtcag ttggtttttc
     2341 gttccccctt tcatcttgat tttggcgagt tccttattcc agatttcgta ggcctggttt
     2401 ttggtttctt cgaagcccca tgcaggaagt tcatttttca ggttgttata agcaccttcg
     2461 gcatctacag gtgaaagagc cactttcacc tcgatgacgg aggtatcctg ggtatcgaaa
     2521 cccacataag cctttatgtt ggttccttct gctttgagaa ttccttcatg cagtgaatcg
     2581 ttgatggcaa tacctttcct cacgaaagga cgcgaaaatt ccatataaaa ataccaaatc
     2641 atatcctgag cccaattcga tgatcttctc aatcctctaa tttgacggtc gccaacaaat
     2701 tctatccatg aatctactac tttgtcgcga tgttgcaaat cgatgatgat ttgtgcctga
     2761 ggcgtaggag ggaaggtata tctgtgataa cccactcggg gggtagcagt taactcagcc
     2821 catacacccg gtttttcgag gaagaccttg taatatcccg ggcaggccga ttctttttcc
     2881 ttgagaaatg gtgacgaata ttcagaattc gaaaacacgg gtttcccaac tactggcatg
     2941 agcagaatat cgccataatc ggaacagccg gtaccactga gggcagtatg tgcaaaacca
     3001 tacaatatgc tgtccgaata atgatatccc gaacaaccat cccatccttc gagacgggtt
     3061 tgagggctaa gttgcaccat tccaaaagga agcgtagcac ccggataagt atgcccgtgg
     3121 cctgcagttc ctatgaaagg atctacccat cgggcgggtt cttctccgga tgagaattct
     3181 ctgtggcatc ccataatcat gaacattatg ggaatgagcc aaacgacaaa aaatctactt
     3241 gttttcatgt ctattcaaat taattcgata aagttcattg agtgtaaaaa agctgaattg
     3301 gcgctggaat tcttgttccg gcaggctggt tctgcaaaat atttccacgt tttggcctgg
     3361 ccatacatga atgaaattgt cagaaaattc ggccggagat tctttcagat aaaactgtat
     3421 gaatgcaata aagacatcac ttttcacgga aatgatataa ccatcgtttt ccctgcgaat
     3481 cgatgttagt aaatgtgctt cgggaaggtt ttgctggctt aataaacctg gcaagcaata
     3541 tgcctttcga atgatagaat cgtcaaaacg taaagtggct acaaataaat aatctcctac
     3601 ttgttgaaat tcgttcaatg gtacagaata aacacaagac gaagaatttg cggaaacatt
     3661 tacttcggct tgcaaaattg gaacaagctt tcccgtgtaa tatctcattt cgaaagtcaa
     3721 tgtaccttta atatctttcg gtaaatcgga gaataggaag attttcactt cctgttcatg
     3781 gatgaaagtt tcaatagcaa ttgggtcgaa aagctggcgt gttttataaa acagggcttt
     3841 tcgattgcct tgaaaatcca tggatgacca cgaaactacc ggccagcagt cgttaagctg
     3901 ccaaaaaagc gaacccatgc aatgcgggcg tgaggctctt tgggcagcca atcccagtcc
     3961 tataccttta gcctgaacaa gttgggttgt atagatatat tcttctaatg tttgaggcga
     4021 aaaaccctca cgttcgagat aaaccgatag gatttcgaat cctaatgaac atttttgatg
     4081 acaacgcaat tgtggagaaa acaaagaatc agcctcggga ggttgaactt ttctgatggt
     4141 agatatttcg ggcatagatt gaaaaccaaa ctcactcata aaacgaggaa ttttctgcaa
     4201 tagaatttcg aaaggttgct ggccccacca aacgccccaa taatgggaat cacccgatac
     4261 catgctttgc gaacgacccc agccaaatct cggagaagat ggaatatagg acgttccagg
     4321 gtcgtactga tgaaccacag caggtaataa gcgatgaaat aaattgagat aatgactcca
     4381 gacagcatct cgaagtttgc tattttggta ttggtttcgc cagccccaat tatgccaggc
     4441 ttcgtctatt tcattgtttc cacaccatag agccaggcaa ggatgatttc gaagccgtct
     4501 tacctgctcg atggcttctt gtcttacatt ttgaaaaaat tcttcatcgc caggatacat
     4561 cgcacaggca aacataaagt cctgccagat aagaataccc atctcgtcgg caagagaata
     4621 aaattcatct ctttcgtaaa ctcctccacc ccaaactctt aacatgttca tgttagcttc
     4681 ttttgcatta cgcaataatc tttcataatc agaatttgta gtttcggtaa gaaaactatg
     4741 cgatggaatg taattggcac ctttcataaa tacaggaacg ccattcagtt tgaaatagaa
     4801 cgaagtccct atgctgtcag gctgtagaac aagctgaatg ttccgaatcc caacccgaat
     4861 atttttatga aaccattctt tcttctctgt tttcaaagaa agttcgaatt catacaaata
     4921 gggcttacct aatccattac accaccaaag ctgtggatga acgaccggaa aatcgaattc
     4981 gaatgaatgg ctgccttttt ctatgttcaa gtccttattc gaaatcaaat ttccatgaaa
     5041 gtaaagttcg aatttggttg agatttcttt gtcgaaatga gaaaagacgg ttatttcaca
     5101 ttttagcaaa gcagtgtcgt gaacaataga ttgagtagtt atagaggcat cttctacttt
     5161 tattccgttg taggccacca aataaactgg tttccaaatt ccacaggtga tgaatgtagg
     5221 gccccaatcc catccgaaat gataagctgc cttacgactc aaattccagc ggccgcctgg
     5281 cagaggatag cctaaagcag cagaatcggc gaggaatcgg tttaaggccg aaaaaaacct
     5341 aacttctaaa aaatttcctc caatttctac aaacggttca atatccaccc gccattccct
     5401 gaacatgttg ttggcctcga gaatgttatg cccattcaga ataacctcgg caaaacagtc
     5461 caaaccttcg aaaacaagct caagattttg aaatgagaag tcattttcat tgagatagaa
     5521 atgcgtatga taactccagt cagtctggcc aatccattgt aattccttct cgttacaggc
     5581 aaagaaagga tcgggaatca aaccgtcacg gtaaaggtcg gtatgaatgg ttccaggtac
     5641 tcgcgccaaa tatcttgcac tgctattagt ctttgaaaat tcccatttca aattgcataa
     5701 atctgttttc ttagattcga aacctgatgc atggcacgac aacccccaca gaacctgcag
     5761 agtaagaaag aaaaaatatc tttttagaaa aatagaatcc atagaataaa tctttcaaag
     5821 ttctaagagc aagtcaggga atatgtagcc acttcaggaa tatgttttat aaagaggcgt
     5881 tttaataaga tgtcatttgt aatatttcga gattgtgtta tttttaattt acataaggat
     5941 gaggagttgg gcgtgagatg gcaagaatca acacataaag aatacaggca gcaggaacga
     6001 tgaaacctaa cattacgttt atatcggcca cagcgccagt gatgggaggt ataagtgcac
     6061 cacccacgat agccataacc atcaatccgc tcaattcgtt agctctttgc ggaatagcat
     6121 ctacagtaat tgaaaagatg agagggaaaa tattggcaaa gcccaatccg accagaaaaa
     6181 ttccacaaaa tgcaactgac ttgctgccga agaataacat cgtcattccc gcaatcgaca
     6241 ataaggcagt gatgaggaga aaacgacggt tcgagaggat tctcaggata aatgatccca
     6301 gcaagcgtcc aaggaaaatt ggtaggaaaa ataatgccca tgccagccag agtccgagag
     6361 tttttatgcc atataccttg tccattagaa taggaatctg ggcgctcatc gagacttcag
     6421 ctcctacata cagaaaaatt cccagtacca tgagggctac gaaaggattt ttcagcagag
     6481 agaaagtaga agaaaaattc gccgacgaag ccgattcgta tcggcattct tcgataggaa
     6541 cagcattaat ccaaagccaa gtaatcaaaa caataaaagc ataaacaggg aagagcagtg
     6601 tccaatcgag gccaaaccag atagacactg ccggaggaag cagaaatccc aaagatgaac
     6661 caatggcctt gatggattga gcgagggaaa gattgctcga ataacgtcct tgtggcgaaa
     6721 catcgcgcat gatgggattt cccgaaacct gcagcatggt agcccctgca cccaaaagaa
     6781 agatcgacac aaataacatg atcaacttca gttgcccgga atcttgcatt tggggggtct
     6841 ttccgtacat cccgaacaag ataggcagca taagcccaat gaaagccaca agcaaccccc
     6901 tcgacaaaac gaatttcttc cctttcttat cttgaatgat tcccaacgga agcgacaaca
     6961 ggccaaacat gatgaacccg cttgtcgtca tgagagaagc aacaaaattt gaaacctcaa
     7021 aagaattctt caccagagaa accagaggcc cgactacatc gccaaagccc atggccagaa
     7081 aagccataaa cacaggtaag gttttattct tcagcattag gtattttagt ttttgatgga
     7141 tgattctcga ataatgatat ctacgggcaa ataaatgctt tgctcatgcg gcaggatttt
     7201 ctctttctga atttgacgaa gtaaaagttc gaaagatttt tcgccaatgc ggtcaatagg
     7261 ctgttcgatg gccgaaatgg aaggatgagt gtaagaaaag taaaccagat cgtcgaagcc
     7321 gatgagagcc acctcttcgg gtatgcgaac agacaacttt ctgagatatt tcaaggtagc
     7381 agaagtaaga ttgttgttca aggtaaaaat agcatcagga agatgagccg actgataaat
     7441 tctttgtatt ccttctttca cggcttggtc gatttgatga aaaggagccc ggatatgcca
     7501 ttcttcgggt attttaatgc ccatctcatt cagagccgac tcgaatcctt taagtcgctc
     7561 attgatggtc gaaatatgtt cgggtgtaat gctgatgaca caaaaattcc gcattccttg
     7621 agagatcaaa tgccgggctg ccatacgagc tcctccgaaa ttatctaccg aaacattggg
     7681 cgaatgcatc tcggtaaata tacgatcgat aagaacatga ggataattct cgtctgctaa
     7741 cttattgaag aatgaaccat cttcctgtga tgatgagaca attaaaccat ctattttgcg
     7801 gcttctcagc atcctaatct gctcgagctc tttgctaatg tcttcgtctg tactgcaaat
     7861 cactacagta taaccattca accatgcgta atcctcaatg cgacgggcta atctcgaata
     7921 aaatagattg gaaatatcgg aaacgatcaa tccaatggtt ttggtttttc ctgttcgaaa
     7981 accctgagcc aattcatcgg ggtgataatt tagctccttt atcttttccc gcactcgttg
     8041 ctgagtctcg gcacttatgc catgctcgtc ggctttattg ttgatgacca gcgagactaa
     8101 agtcttagat acacccagac tttttgcaat ttctgccagt gagatttttt tagccataaa
     8161 ctttatctcc gaatgcaaat gtaaaaaatt ttaactaaaa cgttttagct aataacaaga
     8221 aaaaattatc aatatcatca atcaaataaa gtaattcaat ccgaacgtta gatacgcttt
     8281 tatattcaat gcattctatt ggatgaaata agaaggaatc attacagaat ctccacttca
     8341 ctgatgaaaa tccatgcggg ttggccttca cccgcatgac caagagggca tgtaccgcga
     8401 tttttcattt tcagcctgac gaaacgaaca ttttcgcgca aattctcgaa gccaaataat
     8461 tgaattgcag gcacaggttc ttgccatgaa tgctcgttca ttttttctcc cacggatttc
     8521 cattcctttc catccttcga aagttcacat attgcctttt cgggaaagaa tatccatgag
     8581 cccgaagcct gaagagcatc taccttaatg gaacggattt tcgtcggttt gcccagatcg
     8641 atgattatta tggcatcgtc acccagaaat cctttccagt tgccatcccg gtaatttgtg
     8701 cttccccaaa cgccatctcc caaatttccg ttgtaacgcg aggcacttgg aatctgaaac
     8761 gtcatctttg ctccgaaagc cttatgcaga ctgaattttt tagcgatgat agctcccatg
     8821 cattttccac catgaaagac acctgccttc accgtagccg attcacaaag agcaaatggg
     8881 cccgtataaa ccggcgagct gtccgttgga tcttttccat tggtggtata atggatttcg
     8941 ccgtatgggg cttccgtttc tatggatatt tccacgcaac gtctcacact gtcaggctgg
     9001 ctgatgattc ttgcctgaaa tgcacttctt gcataataaa ttcctaattt atcgtaacac
     9061 ttaaactgat tttcgagtcg acggctgaaa tcttcccagt tacgaaggtt attgggcgac
     9121 caaaccactt cagccaaggc ggcaaggcgt ggaaaaatca tgtattcggc atgctgagga
     9181 gtacgaatat attctgtcca ggtgttggcc tgagcaccaa gaatataatg ggattcctcc
     9241 agactgagtt cctgaggaac aggttcgtaa ctataaactc ttttcagagg gatatagcct
     9301 ccaatggaca ggggttctac ggcaggatca ccctgataat aatcgaagta gcaatggctt
     9361 gctggagtca tgatggcatt gtgcttcagt cgagctgccg ctattccacc ttcctcacct
     9421 ctccatgaca tcacaaccac attgggagcc aatcctccct ctaaaatctc atcccaacct
     9481 atcattatcc gtccgttagc attcaggaat ttctctatgc attgaacaaa ataactctga
     9541 agctcatcta catttttcag gttctcggtt tgcattcgtt gctggcactt cgggcatttg
     9601 ttccattcag acttgtcggc ttcatcgcca ccaatatgaa tgtagatcga agggaacatc
     9661 tccattactt cggtaagcac atctcttagg aaaataaagg cagaatcgtt tcctgcgcaa
     9721 taaatatgtg taattggcca cacactgcct gaaggaacac ccaaattttt gcccgagcat
     9781 gaaaactgcg gataagccgc cagagccgac atcacgtggg caggcatttc tatttcagga
     9841 ataatggtga tattgcgatc ggcagcatac tttacgattt ttctcacttc gtcctgagta
     9901 tagaagccac catacatctc ttgtgtgggt tggcattgta acggtcgagc gttccagtcc
     9961 aaatcttcca tgtcgggacg ccatgcgcca atgcttgtaa gtttgggata tttctcgatt
    10021 tcgagcctcc atccttggtc atctaccaaa tgccagtgaa atacattcat cttgtggaaa
    10081 accatttcat cgagcaaacg atagataaaa tctgcaggca tgaagtgtcg agctacgtcg
    10141 agatgaagtc ctctgtaacg aaatcgtgga taatcaataa tgatgcaggc aggcaatacc
    10201 tgcgaaggat tcgcactcat gagctgattt aaggtttgaa tggcataaat aaggccagcc
    10261 tgagtattgg aacgtagaaa cacattttgt gaagtaattt cgatgccata gccttcagta
    10321 cttaattctt tttcggcggg ttgtataatt tgcaatacaa aatttccttc cagcttttcg
    10381 tgtttaatct gttgtatttc accatcccaa cccgaccaga atttccattg ctggcgggca
    10441 tactgggcaa gctgcatgag cgattcatcc atgtacataa ttttagtttc agggccaaat
    10501 cgaaattctc cggatttcat ttctattttc acaggttgtg gaattatcga aatcacggtt
    10561 tgagagaata cggcaatgga aactgtaaaa gtgaagaaga aaagaagaca attttttttc
    10621 ataaggcttt aagttttatt gaagtcgtat cgacataaac cgataagata atgaggttca
    10681 tttttgacag tttatcagaa taaatgaaaa tttggatatc atcgaaaggc gcatttttat
    10741 tatttaggaa aatgtgcaaa atacggaaat aaaacattac aataaatttt taacacgaaa
    10801 aaaattataa ttttaaagtt aagccaaagt tttttcaaat taatagaaaa agatattgga
    10861 gagagagagc ttgagaaaac cagagttgtt aatcttcatg atcgagcttc gaagcaagta
    10921 ttttatgatc aaatgtagca atttttgcca acaaccattc atttcctcag tcaaaaggct
    10981 gtgaagtgta ttttttaatt gatgatgtcc attgatttaa aaaaatcatt tgcttatcat
    11041 taatctaaat atggttaacc acagagggcg atatagtgaa tatctcagag taggttgatt
    11101 cctcttgaaa aggaaatgga agcagattat tttgtaattt taaaatgata tttgaggtta
    11161 atctggagct ttttcaaaag atataaaaga tgggattagt attttataat tgcaagcggc
    11221 gttgaaaagc aacttcagaa catgttcgta aatagaatta aaaaaagaaa acttacggga
    11281 aaatttatca ttaaaattgt tctttgaaat aaaagttcat taaggggtca ccatctccga
    11341 ttgagggggt caataggact gaaatatcta ttatttattt aaaattccaa tttattagtg
    11401 gtaaagatct tttttcattc ctattccaaa ggcctacttg cactcccttt aatttaacgg
    11461 tacgattaat aaatccaatt tgaataccat tcataaccag ggtactatta aatatgccga
    11521 tcgaaaggcc attaatttca tagtaaaggt tccataacaa attaattgac actccattaa
    11581 tattcttgcc taaactcatc catccagata tagaaacgcc attaacttga tccgtaaaag
    11641 tgcccaataa ggataaaatt aaaccattgt gaattgctct tttaaatgag gtatcttttt
    11701 tgataaattc atcatcaatt attttatttt tgtatacttg cttaaatggt gagttaaaga
    11761 tataaaatgt ttgtaaaata ccttggccgg gtatttgaat attaagtcca tgtgaatatt
    11821 tagtatatgg tttgtcacaa attacttctg aaccaaatgg tcctattgca attccaaata
    11881 tattatttgc ttttgaggga ataaatccaa taacatattt gttcagactt ttattcagaa
    11941 ccggattttt tatttcttct tgacctatgc atttaatgat catcatacat gcaataatag
    12001 tacttaaaat acatttcata ataattcttt ttaaattaag gtggggagtt ccctacctta
    12061 aaattatttt gagccaactt taatatgaaa aaacatttac aactgtactg ccatttctac
    12121 catgagaaaa actgacaggg taaacacctt gcctaactag agtctgtcca tacagtcatc
    12181 aaaattttca gtaaaagtat aaacttctta tgaaagttta cctttttgaa gttattcttg
    12241 gtcaatttta taatttttca gtctaaa
//
LOCUS       DAOORU010000058        12180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_6455_length_12180_cov_5.784082, whole genome shotgun sequence.
ACCESSION   DAOORU010000058 DAOORU010000000
VERSION     DAOORU010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12180
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6455_length_12180_cov_5.784082"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLF20_05165"
     CDS             <1..216
                     /locus_tag="PLF20_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008142766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80427.1"
                     /translation="RGIDQDNARLLLMYAFAAEILNRIDIPALKDRLDDLVKKRLRGE
                     LSICDQCVLHCKNPEKPIVFDIDLSKL"
     gene            241..1455
                     /locus_tag="PLF20_05170"
     CDS             241..1455
                     /locus_tag="PLF20_05170"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005656052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HOX80428.1"
                     /translation="MDVNAVRNDFPILQQKIYGKPLIYLDNAATTQKPNAVMQAIVRY
                     YETLNSNVHRGNHLLSIQATDAMERARNRIASFIHAPHPEEIIFTRGTTESINLVAQG
                     FASSLLQPGDEVIISALEHHSNLVPWQQACLRTGAQLKIIPLDESGQIRLGDFRNLLS
                     EKTRIVAIAHVSNVLGTILPVQDIIDLAHRRGIPVLIDGAQAVQHLPIDVSQLGCDFY
                     AFSGHKIYGPTGIGILYGKGSWLEKIPPFQFGGEMIEIVSFEKTTFNEIPFKFEAGTP
                     NVEGIIGLAAALDYLCQIGLESIFKYETELFEYALHKLSRLDDVRIIGRAKDRCSVIS
                     FLLDHIHPYDTGTLLDQMGIAVRSGNHCAQPLIDLLNLPGTVRVSLSFYNTREEIDIL
                     ITALQKVKDIFQ"
     gene            1469..1906
                     /locus_tag="PLF20_05175"
     CDS             1469..1906
                     /locus_tag="PLF20_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005928823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HOX80429.1"
                     /translation="MQSIQEKADQIVKELNQFDDWLDKYQYLIEKGKELYAAGSEIKN
                     DTNLISGCQSKVWLVSEYRDGRLYFKGESDALITRGLVSMVIQVFSGSTPDEILSFEP
                     KFIEEAGLSQHLSPSRNNGLLSIIKQIKLYAFAWKIKNNYQNK"
     gene            1908..2231
                     /locus_tag="PLF20_05180"
     CDS             1908..2231
                     /locus_tag="PLF20_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HOX80430.1"
                     /translation="MNDSIGKSQLTEEIIKELKILYDPEIPINIYDLGMIYDVEIDDD
                     NNVRILMTLTAPNCPVAESLPADVKKAVENIKGVKSCHVEVTFDPPWDQSMISDAALL
                     ELGLL"
     gene            2325..4223
                     /gene="dxs"
                     /locus_tag="PLF20_05185"
     CDS             2325..4223
                     /gene="dxs"
                     /locus_tag="PLF20_05185"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOX80431.1"
                     /translation="MEYSIGPHLAKINSPEELRMLPEDKLPELCQEIRDYIIEVMSSN
                     PGHLAASLGAVELAVAIHYVFNTPSDKLIWDVGHQAYAHKIITGRRDQFPNNRTYGGI
                     SGFPRMEESEYDAFGTGHSSTSISAALGMAIAAKLRGDARLQHIAVIGDGAMTAGMAF
                     EAMNHAGSTRANILIILNDNGIAIDRNIGALKEYLAQFTASQTYNKFRNRIWNLLGGD
                     SKYGTNTRHIVKQVGNALKSSLLNRSNLFEAMGFRYFGPVDGHDVVKLSHLLHDLKNI
                     PGPKLLHVITKKGKGLEKAELDPTTYHSPGTFNKETGELDKSSCEEKLPPKYQNVFGK
                     TIIELARNNPRIVGITPAMSTGCSLNLMMEVMPERTFDVGIAEQHAVTFSAGLAAAGM
                     IPFCNIYSSFMQRAFDQAIHDVCLQKLHVVFCLDRAGIVGEDGATHHGAFDLAYFRTI
                     PNLIVSAPLNEKELRNMMYTAQLNGIGPFSIRYPRGRGVLNEWRTPFEAIPIGKGQKL
                     KDGTHLAVLSIGHIGNIASEAIAMLQNEGIDCAHYDMRFLKPIDEDLLHEVFTKFKKV
                     ITIEDGTVVGGLGSAVLEFMNDYGYKAQLKRLGIPDRFISHGTIEQLQKECGYDKEGI
                     IKAVKEMM"
     gene            complement(4390..5781)
                     /locus_tag="PLF20_05190"
     CDS             complement(4390..5781)
                     /locus_tag="PLF20_05190"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX80432.1"
                     /translation="MNDKDRPTLLLVYPANPLRKGFLSNRDTKYQPLSLGIIAALTPP
                     HWKVKLIDENFRSFKYYPADLVGITAFSSTVTRAYEIARIYREKGIHTVIGGIHASMM
                     PDEALQCVDTVVIGEAESVWPKVISDFEAGCIQRIYKGELLEMINSPKPRRDLFHPGY
                     VFASIQTTRGCPFDCSFCSVSAFNGRKYRMRPIEEVLDELEEIPQNNVFFLDDNIVGI
                     TKEHQERAIELFRRIIERRIKKNWISQASINLADNPEVLKWAARSGCRMIFIGVESEA
                     EDGLRESNKRINLKYGVSSYEKVFQKVHQHGIAVIAGFMFGWDSDTAEKMQQRVEYIR
                     HSQADSIQTSIVTPLPGTRLYHKLMAENRIILKNYPDDWQRYDYWEIVHSLPMMAKQE
                     FETEMQRVYRRIYEPTFLRQKFFRSIWRLKSIITAYWSYMSNWNYRNMYIHDGKKASN
                     FLLDPFPFFRKKL"
     gene            complement(5784..7166)
                     /locus_tag="PLF20_05195"
     CDS             complement(5784..7166)
                     /locus_tag="PLF20_05195"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX80433.1"
                     /translation="MNKKTLILINPSNQYRRGFALRQQSRQVPLGLGIIAALTPPNWK
                     IIILDENIKPFRFREADLVGITTFTSTAQRAYEIAAVYRGKGIPVVFGGIHVSMLPDE
                     ALQYGDAVVIGEAETSWPNVMSDFEAGRLQRIYRGPHADLSQCPIPRHDLFHPSYLFA
                     SIQTSRGCPMDCDFCSVPVFNGHSYRLRPVEAILDEMEALPQKLIYFVDDNITGYSKA
                     SEKHAQELFEGMIRRGLKKEWFAQASLNISQNPELLKLAARSGCKMLLIGIEAENENA
                     LRDTNKHVNLTLGTKTYKHAFRTIHQAGIAVLGAFIYGMDSDTPSSLQARTKFIVHSS
                     VDVVQASAITPLPGTRLFKRLQQENRILANHPPHSWKYFHFSDVVFRPAEMQAHELAD
                     NLWMAYQKIFSLKTLRMKFLRTLWNTKNLRTSVWAWNSNLNYRAVSMEEPPAVGHDIQ
                     NTSSEADYIH"
     gene            7355..7597
                     /locus_tag="PLF20_05200"
     CDS             7355..7597
                     /locus_tag="PLF20_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOX80434.1"
                     /translation="MALNIHDFIERIEDEFDELEPGKLKPDSVFRQMFDWNSINALVL
                     IAMVKTEYNVTINADDLAKSKTVRDVFKIIETRYHE"
     gene            7614..8669
                     /locus_tag="PLF20_05205"
     CDS             7614..8669
                     /locus_tag="PLF20_05205"
                     /inference="COORDINATES: protein motif:HMM:NF020130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HOX80435.1"
                     /translation="MATFSVPGIKVSGLAACVPRKEVSNYDYRWVTVKEREQLVKTIG
                     VEKRRHVEKGMATSDMCQKAAQSLLEGLHWNPAEIELLIFVSQTRDYLIPQTSNILQH
                     KLGLPTTAISLDISLGCSGYVYGLAVASAMMNSFGISKALLLVGDISSLNSAYRDKST
                     YPLFGDAGTATALERLEDAPQMYFSLEGDGSGYKAIMIPDGGMRNIVSKKSFEYKKIA
                     PGIYRTPLHIALDGIEVFNFSLREVVPNINKLLAFSGQELANVDYILFHQANRLINES
                     LRKMLKIPKEKVPYSLNKFGNTSSASIPLTIVSELRNIVLENDLLLLLSAFGIGLSWA
                     TATIEFHRIYCSELLEL"
     gene            8673..9410
                     /locus_tag="PLF20_05210"
     CDS             8673..9410
                     /locus_tag="PLF20_05210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX80436.1"
                     /translation="MLEAFQLTGKNILITGASSGLGRQTAIQASKAEANLILTGRNLE
                     RLDETFRQLKGDEHLIIPADLTHEDELNHLVDTVPPLNGVVYSTGVSILKPVRFLESA
                     DIAENFSIGFNAPVLLTSRLLAKKKLVSGNCSLVFISSISIKYPFVGGSMYVSVKAAL
                     EGFSRVLALEMAPKGIRVNCVAPAFVKGPMLEQTREATSEETIQQIKARQPLGLGDPV
                     DVANVIIFFLSDASRWITATSLILGGG"
     gene            9428..10354
                     /locus_tag="PLF20_05215"
     CDS             9428..10354
                     /locus_tag="PLF20_05215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOX80437.1"
                     /translation="MNQPEYSVVVPVFNSQESLLELFEGIAAAFGELNTEFETIFVDD
                     RSTDNSWEVLKNLKQEHPQHIKIIRLSRNYGQHNATLCGLMFAKGEAIITIDDDLQTP
                     PHEIITLVRSFEESSCDVIYGIPYGKKAHHFYRNLGSSVIKKSSRNLRNTPGEGSSFR
                     LLRRSITDKIVQHSLFFVFIDEILLWYTDDIRFVEVAHVPRKYRRSGYSFGKLWHLLT
                     NLVVFYTDAPLKIMVYGGLFASFVSFILGIIYIVKKLFFNVPLGYTSLIVAILFSTSI
                     ILFSLGIIGEYLSRIYKVQNRKPPFSIDTVIE"
     gene            10365..11054
                     /locus_tag="PLF20_05220"
     CDS             10365..11054
                     /locus_tag="PLF20_05220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX80438.1"
                     /translation="MKLVKYDIVLERLKEADIELVRQHRNSEQIRRTMEYREYITPEM
                     QREWFESVNKNIDQLYFIIHYRSEKIGLINAKNIDWEKQILESGIFLWESKYYETFLP
                     AIVSIMITDMCFQLLGWDVIYAHILRTNDKAIKYNKSLGYVLCEDQEDKENQLYRLRP
                     ESFHRKTQKLRKAISNLYSEKDEAYLIIEPSDIANDILSQLEPFFQLVRRQRGDFESY
                     FNADGSITFTF"
     gene            complement(11044..12096)
                     /locus_tag="PLF20_05225"
     CDS             complement(11044..12096)
                     /locus_tag="PLF20_05225"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOX80439.1"
                     /translation="MKIVQSQLFFILGRPRSGTTLLRTLLDAHPQVKVPPEYPVALQL
                     YRKYGKIRQWDDKTLKAFKKDFRSPLSSQNWKYEFLRIDERQLDEDLKNLLPNATFEE
                     IFKCYYANYTSVVSGKEIITHIGDKNPIFATHAFRLRKIFPKAKFIFIFRDYRDNFLS
                     VKKFRFEAPIIALQAYRWKYVARLAYQFEKKFPEQTLIIRYEDLTRKPREILSHTCTF
                     LGLPYDETMLDFWTYSSKYSEFVDPELLNQFHSGLQSPIQTSSSGKWMKEMKQKEIKT
                     ADQVVGRYAERLGYQRVDKKFHPITWLKTLPWQLYGFILYRMMSLAEFLPASLRQKFS
                     MTLPKLTNIYHKWFKK"
BASE COUNT         3509 a         2496 c         2598 g         3577 t
ORIGIN      
        1 agaggcattg atcaagataa cgcacgactt ttgttgatgt atgcttttgc tgccgaaatc
       61 ttgaatcgta tcgatatacc agctcttaag gatcggctcg acgacctagt caaaaagcgt
      121 ctgaggggcg aattatctat ctgcgatcaa tgtgtccttc actgtaaaaa cccagaaaag
      181 cccattgttt tcgatattga cttgagtaag ttgtaagttt taaaatattt tgccgtgaaa
      241 ctggatgtaa atgccgtcag aaatgatttc cctatacttc agcaaaagat ttatggaaag
      301 ccattaatct atctcgataa tgcagccacc acacaaaagc caaatgcggt tatgcaggcc
      361 attgttcgtt attatgagac tctaaacagc aatgtgcacc gtggaaatca tctgttaagc
      421 attcaggcaa cggatgcaat ggaaagagcc cgaaatagga ttgcttcatt tatccatgca
      481 cctcatcccg aagaaattat ttttacgcgc ggcactaccg aatcgatcaa tttagtggct
      541 caaggatttg ccagttcact gcttcaacca ggcgacgaag taattatctc cgccctcgaa
      601 catcattcga acctcgtccc ttggcaacag gcttgcttac gtacgggtgc acaactaaaa
      661 atcattcctc tcgacgaatc cggccaaata cggctcgggg attttcgcaa cctcctttcc
      721 gagaaaaccc gcatcgtggc gatagctcat gtgtctaatg ttttaggaac gattctgcct
      781 gtccaggata tcattgatct ggctcatcga cgcggcattc cagtattaat agatggtgct
      841 caggctgtgc agcatcttcc tatagatgta agccaactcg gttgcgattt ttatgccttt
      901 tccgggcata aaatttatgg tcctacggga attggaatcc tttacggaaa aggatcgtgg
      961 ctcgaaaaga ttcctccgtt tcaatttggc ggtgaaatga tcgaaatcgt tagtttcgaa
     1021 aaaaccacgt tcaacgagat accttttaag ttcgaggccg gcacccctaa cgtggaaggt
     1081 atcatcggcc ttgccgccgc acttgattat ctctgccaga ttggactcga aagcattttt
     1141 aaatatgaaa ctgaactttt cgaatatgcc ctccataagt tgagccgatt ggatgacgtt
     1201 cgaatcattg ggcgggcaaa agaccggtgt tcggtcatct catttctgct cgatcatatt
     1261 catccttacg acacaggcac cttgctcgac caaatgggta tagccgtgcg ttcaggaaat
     1321 cattgcgctc agcctttgat cgacctcctg aaccttcctg gaactgtcag agtttcatta
     1381 tcattttaca atacaagaga agaaatcgac attttaatta cagccctgca aaaggtgaaa
     1441 gacatttttc agtaaaaact atttatccat gcaaagtatt caagaaaaag ccgatcagat
     1501 agtcaaagaa ctcaatcagt ttgatgattg gctcgataaa tatcaatatt tgatcgagaa
     1561 ggggaaggaa ctttatgctg cagggtccga aattaaaaac gatacaaatc tgatttcggg
     1621 atgtcagtcg aaagtgtggc tagtttcaga ataccgcgac ggaagactct attttaaggg
     1681 tgaaagcgac gccctgatta cacgtggcct cgtcagtatg gtcattcagg ttttttcggg
     1741 cagtactccc gacgagattc tttcgttcga acctaaattt attgaagaag ccggcctaag
     1801 tcaacacctt tcccctagtc gcaacaacgg tcttctttcg ataataaagc aaatcaagct
     1861 ttatgctttt gcatggaaaa taaaaaataa ctatcaaaat aaataatatg aacgattcta
     1921 ttgggaaaag ccagctaacg gaggaaatca tcaaggagct gaaaatattg tatgatcccg
     1981 aaataccgat caacatctat gatcttggca tgatttacga tgtcgagatc gacgacgata
     2041 ataacgtacg cattctgatg acgcttacgg ctcctaactg ccctgtagcc gaatcattgc
     2101 ctgcggatgt aaaaaaagca gttgaaaaca tcaagggcgt gaaaagttgc catgtagaag
     2161 ttacctttga tccaccctgg gatcaaagca tgataagtga tgctgctctg ctcgagcttg
     2221 ggcttcttta attgctttgt ctattctttc ttttcgttat tttgtaaacc taaaaggttt
     2281 gtaagtgtaa ttttgcaatc taaagatttt gttggaatat tgagatggaa tatagtattg
     2341 gaccccatct ggctaagata aattcgcccg aagaattgcg catgcttccc gaagataaac
     2401 tgcccgagct gtgccaggaa atcagggatt acattataga agtaatgtcg tctaatcctg
     2461 ggcatctggc ggcaagtctc ggagccgttg agttggcagt agccattcat tatgttttta
     2521 atactccttc cgataaattg atctgggacg tcgggcatca ggcttatgct cataaaatca
     2581 ttaccggccg tcgcgatcaa tttccgaaca acagaactta tggcggtata agtggttttc
     2641 cccggatgga agaaagtgaa tacgatgcct ttgggacagg gcattcttcg accagtatct
     2701 cggctgctct tggaatggct attgctgcaa aacttcgagg tgacgcacgc cttcagcata
     2761 tagccgtgat cggcgatggt gccatgactg caggaatggc ctttgaagct atgaatcacg
     2821 ccggaagcac ccgagcaaac attctgatca tcctcaacga caatggcatt gcgattgata
     2881 gaaacatcgg agcactgaag gaatatctcg ctcaattcac tgcctcacag acttataata
     2941 agtttagaaa caggatttgg aacctattgg gcggagatag taaatatggc acgaatactc
     3001 gccacatcgt caaacaggta ggtaatgctc tgaaatcatc tcttttgaac cgcagcaatc
     3061 ttttcgaggc tatgggattc agatattttg gtccggtcga tggacacgac gtggtaaaat
     3121 tatcacattt actccatgat ttaaaaaata ttccaggacc taaactattg catgtaatca
     3181 caaaaaaggg caaaggactc gaaaaggcag aattagatcc tactacttat cattccccag
     3241 ggacattcaa caaggaaacc ggcgagctcg ataaatcgag ctgcgaggaa aaactacccc
     3301 ctaaatatca aaatgtattc ggaaagacta tcatcgaact tgcccgcaac aatccccgaa
     3361 ttgtagggat tacgcctgcc atgtctacgg gttgctctct caacttgatg atggaagtaa
     3421 tgcccgaaag aactttcgat gtcggcatag ccgaacaaca tgccgttacg ttttcggcag
     3481 ggcttgctgc tgctggtatg attcctttct gcaatatcta ttcttcgttt atgcaaagag
     3541 ctttcgatca ggcaattcat gatgtttgtc tgcaaaaact ccatgtggtt ttttgtcttg
     3601 atagagctgg aatagtaggt gaagacggag caacgcatca tggagccttc gatctggcct
     3661 attttagaac aattcccaat ctcatcgttt ctgcaccctt aaacgaaaaa gaacttcgca
     3721 acatgatgta cacagctcaa ctcaatggaa taggcccttt ttctatcaga tatccgcgag
     3781 gccgcggagt attaaacgaa tggagaaccc ctttcgaagc catacccatt gggaaaggtc
     3841 aaaaactaaa agatggtaca cacctcgccg ttctttctat cggacatatt ggaaatattg
     3901 cttctgaagc tatcgctatg cttcaaaatg aaggaatcga ttgtgcccat tatgatatgc
     3961 gattcttgaa acctatcgat gaagatttgc ttcatgaagt ttttaccaaa tttaaaaaag
     4021 tcataaccat cgaagatggc acagtcgttg gtgggctggg aagcgcagta ctcgaattta
     4081 tgaacgatta tggatataag gcccagctta aacggctcgg tattcccgat cgcttcatca
     4141 gccatggcac catcgaacaa ttgcagaaag agtgtggcta cgacaaggaa ggcatcatca
     4201 aagccgtgaa agaaatgatg taaatgttct atcgccattt ccctcgttta ctgagtttag
     4261 ctgagcattg cgtgaaatgt gaggaataag gaaggaaaga atcaagttgg agtggaagtt
     4321 tagtaaagcc cagttggcca aagagatgag taaaccaaga gagtggggaa cgaggcgact
     4381 tggtcttttc tataactttt tacgaaaaaa tggaaatgga tcgagaagaa agttgcttgc
     4441 ctttttccca tcgtgaatat acatgttacg atagttccaa ttgctcatgt aactccaata
     4501 ggcggtaata atacttttca acctccaaat cgacctgaaa aatttttgtc ggaggaacgt
     4561 aggttcataa atccgtcgat ataccctttg catttctgtt tcaaactctt gcttggccat
     4621 cattggtaga ctgtgaacaa tttcccagta atcataacgt tgccagtcat caggataatt
     4681 tttcaagata atacggtttt cagccattaa tttatgataa agccgtgttc ctggtaatgg
     4741 ggttacgatg ctggtttgaa tggagtcggc ttgagaatga cgaatatact cgactctctg
     4801 ttgcattttt tcggctgtat ccgagtccca accgaacata aagcctgcaa tcactgctat
     4861 accgtgttga tgaacttttt gaaaaacttt ttcataggat gaaaccccat atttgaggtt
     4921 gatgcgttta ttcgattccc ggagtccgtc ttctgcttca ctttctactc caatgaagat
     4981 cattcgacag ccacttcggg ctgcccattt gagaacttcg ggattatcgg caagattaat
     5041 ggatgcctga ctaatccagt tttttttaat tctgcgttcg atgattcttc tgaatagctc
     5101 tatggctctt tcctgatgtt ctttggtgat gccaacaata ttatcgtcga gaaagaagac
     5161 attattttga ggaatttctt ctaattcgtc caatacttct tcgatagggc gcattctata
     5221 cttgcgtcca ttgaaagccg atacggaaca aaaactacaa tcgaaagggc agccacgagt
     5281 ggtttgaatg gaagcaaata cataacccgg gtggaacaaa tcgcgccgtg gcttcgggct
     5341 attgatcatt tcgagtagtt ctcccttata tatgcgttga atacatccgg cttcaaagtc
     5401 ggagattact tttggccata cactctcagc ctctccaatt accactgtat ccacacattg
     5461 cagagcttca tcaggcatca tgctggcatg tattccgcca ataaccgtgt gtataccctt
     5521 ctccctgtaa attcgagcaa tttcgtaagc ccgtgtaact gtgctcgaaa atgcggtaat
     5581 cccgaccaga tcggcagggt aatacttgaa agaacgaaaa ttttcatcta tcagcttcac
     5641 tttccagtga gggggagtta aggcggcaat aattcctaaa ctcagcggtt gatattttgt
     5701 atcgcgattc gataaaaatc ctttcctgag cggattggct ggataaacca gcaatagggt
     5761 ggggcgatct ttatcgttca ttttcaatga atataatcag cttctgaaga agtgttttgg
     5821 atatcatggc caacggctgg aggctcttcc atggaaactg ccctgtagtt gaggttgctg
     5881 ttccatgccc aaactgaagt tcttaagttt ttagtattcc agagtgtacg cagaaatttc
     5941 attctcaatg ttttcaacga gaaaattttt tggtaagcca tccaaaggtt atcggcaagt
     6001 tcatgagctt gcatctctgc cggccgaaaa actacgtcgg aaaaatgaaa gtatttccac
     6061 gaatggggag gatgattggc cagaatcctg ttttcttgct gaagtcgttt aaaaagacgg
     6121 gttcctggca atggagttat ggcactggcc tgcaccacat ctaccgagga atggactatg
     6181 aatttggtac gggcttgaag cgaggaaggt gtgtcagagt ccatgccata gataaaggcg
     6241 cctaaaacgg ctattcctgc ctgatgtatt gtccgaaaag catgcttgta agttttggtc
     6301 cctaaggtta aatttacatg tttatttgta tcacgaaggg cattttcgtt ctcggcttcg
     6361 ataccaatca acaacatttt acatccgctg cgcgctgcca gtttcaaaag ttctggattt
     6421 tgcgaaatgt ttaagcttgc ctgcgcaaac cattcttttt ttaatcctct gcgtatcatt
     6481 ccttcgaaca gctcttgtgc gtgcttttcg gatgccttgc tataaccggt aatgttgtca
     6541 tctacgaaat aaataagttt ttgtggtaga gcctccattt catccagtat ggcctctacg
     6601 ggtcgcagcc ggtagctatg gccgttgaaa accggtaccg aacagaaatc acaatccatg
     6661 gggcagccac ggctggtttg aatactggcg aataggtaag aaggatgaaa gaggtcgtgt
     6721 cgtggaatgg ggcattgtga aagatcggca tgtgggccgc gatagattct ttgaagccgt
     6781 cccgcttcaa aatcactcat aacgtttggc catgaagttt ctgcttcccc aattaccacg
     6841 gcatcaccat actggagagc ttcatccgga agcattgaaa catgaatgcc tccaaaaacc
     6901 actggaattc cctttccacg ataaactgcg gcgatttcat aggcgcgttg agcagtggaa
     6961 gtaaatgttg taatgcctac aagatcagct tcgcgaaaac gaaatggttt aatattctca
     7021 tccagaataa taattttcca attaggaggt gttaaagcag caataatccc caatccgagt
     7081 ggcacctgac gagattgctg gcgcaaggca aatcctcgcc tgtactgatt ggaaggatta
     7141 atcaagatta aagtcttctt attcaacgat taaatgaaaa tattacgacc gctaaaatta
     7201 cgataattta taaagatgag tcggtggagt attttctaat aagggagcat tggcatggct
     7261 acagcaaata gtgttgtatt catccttcat tgtgaggaat aagagtaatc ttgataaaaa
     7321 ccttaaattt gccaaccaat ttaattttct cacgatggct ttgaatatac atgattttat
     7381 agaaagaatc gaagacgaat tcgatgaact cgagccaggc aaattgaaac ccgatagtgt
     7441 gtttcgtcag atgttcgatt ggaattcgat caatgcattg gtactcattg caatggtaaa
     7501 gaccgaatac aatgtaacca ttaatgcaga tgatcttgct aaatcgaaga ctgtaagaga
     7561 tgttttcaag attatcgaaa cgcgatatca tgaatagtta ataattttaa tgaatggcta
     7621 cttttagtgt tcccggaatc aaagtatcag gtttagctgc ctgtgtgccc agaaaagaag
     7681 tttccaatta tgattaccgc tgggtaacgg ttaaagaaag ggaacagctc gtaaaaacca
     7741 taggcgttga gaaacggcgt catgttgaga aaggcatggc tacaagcgat atgtgccaaa
     7801 aggctgctca aagtttgctg gagggtcttc actggaaccc cgccgaaata gaacttttga
     7861 tcttcgtatc gcaaacccgc gattatctta ttccacagac ttcgaatatc cttcagcata
     7921 agttgggatt gccaactact gccatttcac tcgacatcag tttgggatgc tcgggttatg
     7981 tatatggatt ggcggtagct tcggccatga tgaactcttt tggtatatcg aaggcactgt
     8041 tattagtagg tgatatttct tcgctcaatt ccgcctatcg cgataaaagt acttatcctt
     8101 tatttggaga tgcaggaaca gcaactgcac tcgagcgtct cgaagatgcc ccgcagatgt
     8161 atttcagcct cgagggcgat ggctcgggtt acaaggccat catgattcca gatggaggga
     8221 tgcgaaacat agtttcgaag aaatcattcg aatacaaaaa gattgcccct ggaatttatc
     8281 gcacgccatt gcatattgct ctcgacggaa tagaagtctt caatttcagc cttcgcgagg
     8341 tggttcctaa catcaataaa ttactcgctt tcagcgggca ggaacttgcc aatgtagatt
     8401 atatcctttt tcatcaggca aatcggttga tcaacgaatc tctgcgcaaa atgctgaaaa
     8461 ttcctaaaga aaaagttcct tattcattaa acaaatttgg gaatacgtct tcggcaagca
     8521 tacccttgac cattgtttca gaattacgaa acatagtgtt ggagaatgat cttcttttac
     8581 ttctctcggc ctttggaata ggtttgtcgt gggcaacggc tacgatagaa tttcatagga
     8641 tttattgttc tgaactatta gaattatagg atatgctcga ggcatttcag cttactggga
     8701 aaaacattct aataaccgga gcctcgtcgg gcttaggtcg tcaaactgcc attcaggcga
     8761 gcaaagcgga agctaacctc atacttacgg gacgtaatct tgaaaggctc gatgagactt
     8821 ttcgacagct taagggcgat gaacacctca ttattccggc cgatctcact catgaagatg
     8881 aattgaacca tttggttgat actgtgcctc ccttaaatgg ggtggtttat agtacgggtg
     8941 tcagtattct gaaacctgtc cgctttctgg aatctgccga tattgccgaa aatttttcca
     9001 tcggattcaa tgctcccgtt ttattaactt cacgactgct tgcgaaaaaa aaactggtca
     9061 gtggcaattg ttcgctggtt tttatttctt ctatttctat taaatatcct tttgtaggag
     9121 gaagcatgta tgtaagcgtc aaggcggcgt tggaaggttt ttcgcgtgtg ttggctcttg
     9181 aaatggctcc taagggaatt agggtaaatt gcgtagctcc tgcctttgtt aaaggaccta
     9241 tgctcgaaca aacccgagaa gcaacaagtg aggaaaccat tcaacaaatt aaagcgcgtc
     9301 aaccgcttgg actgggtgat ccggtggacg tggccaatgt catcattttc ttccttagtg
     9361 atgcttcacg ttggattact gctactagct taattcttgg aggtggttaa tctttgataa
     9421 cgaatttatg aatcagcctg aatatagcgt cgtagttcca gttttcaaca gtcaggaatc
     9481 tcttttggag ctctttgagg ggattgccgc agcctttggt gagttgaata cagaatttga
     9541 aaccattttc gtggacgacc gaagcaccga taatagctgg gaagtactga aaaatctcaa
     9601 acaagagcat cctcaacata tcaaaatcat ccgactatcc cgaaattacg gtcagcataa
     9661 tgccacactt tgcggactta tgtttgccaa gggcgaagcc attatcacca tagacgatga
     9721 tttgcaaact ccacctcatg aaattattac cctggttcga tctttcgaag aatcttcttg
     9781 cgatgtgatt tatggtatcc cctacggtaa aaaagcccat catttttata ggaatcttgg
     9841 cagttctgtt attaaaaaat cttcacgcaa tttaagaaac acgcctggtg aaggttcctc
     9901 attccgtctg cttcgccgtt ccattaccga taagattgtt caacattccc tgtttttcgt
     9961 ttttatagat gaaatcttgc tctggtacac cgacgatatt cgttttgtcg aagtggctca
    10021 tgttcctcga aaatatcgtc gttcgggata ctcttttgga aaattatggc atttgttaac
    10081 caatctggtg gttttttata ccgatgctcc actcaaaatc atggtttatg gtggactttt
    10141 cgcctctttc gtgagtttta tccttggaat tatctatatc gtcaaaaaat tgttcttcaa
    10201 cgtcccattg ggatatacct cattgatcgt tgctatttta ttttcaacaa gtattatcct
    10261 gttcagtttg ggaattattg gggaatatct cagccgtatt tataaggttc aaaatcgtaa
    10321 acctcccttt tctatcgata ccgttataga gtaaatgaat taagatgaaa ttggtaaaat
    10381 acgacatagt actcgaacgc ctgaaggagg ccgatatcga gttagtaagg cagcatcgca
    10441 atagcgagca gattcgtcga actatggaat atcgcgaata tattactccc gaaatgcaac
    10501 gtgagtggtt cgaaagcgtc aataagaata tcgaccagtt atatttcatt attcattatc
    10561 gatcggagaa aatcggcctg attaatgcaa agaatatcga ctgggagaaa caaatccttg
    10621 aatcgggtat ttttctttgg gaatcgaaat attatgaaac cttcctacct gccatcgtct
    10681 ccatcatgat cacggatatg tgttttcagc ttttgggatg ggatgtgatt tatgctcata
    10741 ttttaaggac aaatgataag gccataaagt acaataagag tctgggatat gttttgtgtg
    10801 aagatcagga agataaagaa aatcagttat atcggctgag gccagaatcc ttccatcgaa
    10861 aaacgcaaaa gctccgaaaa gccatttcga atctttattc tgagaaagat gaggcatatt
    10921 tgattatcga accttctgat attgccaatg acatcctttc ccagctcgag ccatttttcc
    10981 aattggttcg acgtcaaaga ggggatttcg agtcgtattt caatgctgat ggtagcatta
    11041 cttttacttt ttaaaccatt tatgataaat attggttagt tttggcaatg tcatagaaaa
    11101 tttttgcctt aaagaagctg gtagaaattc ggctaaagac atcattcgat ataagatgaa
    11161 accatataat tgccaaggca aagtctttag ccaggtaatc ggatgaaatt ttttatctac
    11221 tctttgatat cccaatcttt cggcatatct tccaactact tgatcggcag ttttgatttc
    11281 cttttgtttc atttctttca tccatttacc actcgaagag gtttgaatag gagattgaag
    11341 tcctgaatga aattgattta ataactcagg gtcaacaaat tcggaatact tcgatgaata
    11401 agtccaaaaa tcgagcatgg tttcatcata aggcagtcca agaaaagtac atgtatggct
    11461 taagatttcc cgaggctttc tagttaaatc ttcatatctt atgatgagag tttgctctgg
    11521 aaattttttt tcgaactgat aagccaaccg agccacgtat ttccatctat aagcctgcaa
    11581 ggctataata ggagcctcaa atcggaattt tttaaccgaa agaaaattat cccgataatc
    11641 cctaaaaata aaaataaatt tggcctttgg aaatatcttc ctcaaccgaa aagcatgggt
    11701 agcaaaaatg gggtttttat cacctatatg ggttatgatt tccttgccgg atactactga
    11761 tgtataattg gcataataac atttgaagat ttcttcaaaa gtggcattgg gcaaaagatt
    11821 cttcaagtct tcgtccaatt gcctttcatc tatgcgcaaa aactcatatt tccaattctg
    11881 cgaagataga ggtgagcgga agtccttttt gaatgccttg agtgttttat catcccactg
    11941 ccgaatcttg ccatatttcc tgtaaagctg caaagcgaca gggtattcgg gtgggacctt
    12001 cacttgcgga tgggcatcca gtaacgttct caacaaggtg gttcccgaac gaggccttcc
    12061 taaaataaaa aacagttgag attgtactat tttcatttat aagggataac ctttttggta
    12121 aaatgtttaa tcccaaaccc taaattttta ggtaatagat attcccattt tctccagtag
//
LOCUS       DAOORU010000059        12163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_6466_length_12163_cov_5.801619, whole genome shotgun sequence.
ACCESSION   DAOORU010000059 DAOORU010000000
VERSION     DAOORU010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12163
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6466_length_12163_cov_5.801619"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PLF20_05230"
     CDS             complement(<1..962)
                     /locus_tag="PLF20_05230"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOX80440.1"
                     /translation="MKMQKTLITGAKMINEGNIQEMDILIEEPYIASIMRAGTNAGKA
                     CVIEAQGKYLLPGIIDEHVHFREPGLTHKADIYTESRAAVAGGVTSFMDMPNTIPQTT
                     NHKTLEWKLKRASKSSLANYAFYLGATNDNLNEILDIDPAMIPGVKVFMGASTGNMRI
                     DDEETLINIFSKAPCLIAVHCEDEDLIRKNLAYYQSIYAKDIPLEYHSLIRSADACQK
                     STQKAIALAREYDSRLHVLHLSSVEETKLFTNKLPSNQKQITSEVCIHHLWFSNIDYK
                     EKGTKIKWNPSIKTPADKKALWEALLDDRIDAVATDHAPHTLHE"
     gene            complement(1004..1759)
                     /locus_tag="PLF20_05235"
     CDS             complement(1004..1759)
                     /locus_tag="PLF20_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOX80441.1"
                     /translation="MLHNLCQVSDSLVIIPTYNEKENIERIIRKVFSLEPAFDILVVD
                     DNSPDGTARIVKQLIQEFPERLFIEERSGKLGLGTAYIHGFKWALEKDYQFIFEMDAD
                     FSHNPNDLIRLRQAVAENSADLAIGSRYISGVNVVNWPIGRVLMSYLASKYVRLITGI
                     KVHDTTAGFICYSRSVLQTINLDEIKFTGYAFQIEMKYSAWKLGFTIIEIPIIFTDRT
                     AGESKMSRGIFKEAALGVIKLRFKKIVPNKRQE"
     gene            complement(1889..2947)
                     /locus_tag="PLF20_05240"
     CDS             complement(1889..2947)
                     /locus_tag="PLF20_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX80442.1"
                     /translation="MLELFNIKKRLGDFLLEIPSYCLEEGEYFVFAGPSGAGKSQLLE
                     LIAGLSFPDEGKIIFDGIDITSLPTQKRKIGMVFQDMALFPHLTVKQNISFPMRAKKK
                     EPIYVKNEIMKLAHAMSIEKLLDHYPHQLSGGEARRVALARTLASQPRLLLLDEPLSE
                     VDTSLKVALRRLLRTINQQGVTIIHVSHDYEDALTLASKMAIINDGKIIQVGQPSEIF
                     LKPASPFVAQFAGIRNFFHCKFRSLHNSSLSLAVINDEKQIFITGTISSTHGSIIIPA
                     RDIVLSEQKLESSARNNFPGIVKEIIPTREGLEVIVDCSFPLAAIVSTASVIQLKLEV
                     GSKVWVSFKASSVQFLEG"
     gene            complement(2940..3734)
                     /locus_tag="PLF20_05245"
     CDS             complement(2940..3734)
                     /locus_tag="PLF20_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX80443.1"
                     /translation="MPYKSRNFTTFSLVMAILGGLVLLFILAPLLGMFLRSSPNQIVN
                     TIKEPEVSSSLAVSLFTAFVATFIGAIGAIPLAYLLARKQFRGKLVVLGLIDLPVVIP
                     HTAAGIAILGVISRNSLIGSAAARAGFDLIGTPISISCAMAFVSLPFLINAARSGFEA
                     VPIRLEKAASNLGASSVRTFFTVSLPLAWRSILSGLVMMFARGMSEFGAVIIVAYHPM
                     TAPVLIFERFNNYGLNYANPLAAILILVSLVFFILLRLLAKPAKNA"
     gene            complement(3889..5094)
                     /locus_tag="PLF20_05250"
     CDS             complement(3889..5094)
                     /locus_tag="PLF20_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007664482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOX80444.1"
                     /translation="MKLLKEKSIIWLLSIALLMPFFVKAQDTKTDSSYQFTLVKQLPY
                     TPVKNQFRSGTCWSFSAISFIESELLRKGKGEYDLSEMFCVRDAYEKKAEKYLRMGGT
                     TNFSGGGEFHDVIHTLRDRGLMPEEAYPGLDYGEKNHVHGELDAVAKAYMDALIKNPN
                     KKISTAWMNGFKGIMDAYLGHVPENFVYKDKLYTSRSFAESLGLHSEDYVCLTSFTHH
                     PFYKTFILEVPDNWAWGEIYNVPLDELMEIIDYALDKGYTVGWASDVSEKGFAYNKGV
                     AVIPETDVTELSGAEKARWEKLTEKERNSQMYNLDRVVPEKKVTQEMRQKEFDNLQTT
                     DDHGMHIVGYGNDQFGHKYYYVKNSWGGDNKFNGFFYASVPFVRLKTIDILVHKEAIP
                     TAIKKKLGI"
     gene            5285..5902
                     /gene="cysC"
                     /locus_tag="PLF20_05255"
     CDS             5285..5902
                     /gene="cysC"
                     /locus_tag="PLF20_05255"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HOX80445.1"
                     /translation="MMPNTQQNLFPSSSSERNKMESLLGQKSVVIWFTGLSGAGKTTL
                     GRILRDELFSRGFIAQMLDGDNLRSGLNKDLGFSVEDRIENIRRAAEVSKLFMESGII
                     SINCFITPTEAIRAMARNIIGKENLLEVFVDCPIDICQQRDTKGLYLKAKEGLINDFT
                     GVNSPFEIPQYSDIHLFTHIMHPYRCLEVLMEYILPRITYHKTGK"
     gene            complement(5904..6668)
                     /locus_tag="PLF20_05260"
     CDS             complement(5904..6668)
                     /locus_tag="PLF20_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOX80446.1"
                     /translation="MKLISYNVNGLRSALSKGLLEWLEQERPDIFSLQELKAQPDQIP
                     DEEFRRLGYHTYWHCAEKRGYSGVGLISLREPDRVVKGMGKTEYDREGRFLRADFGEI
                     SVISVYHPSGTTGEVRQDFKMQWLEFFLQYVNNLRKERPYLIISGDFNICHKPIDIHD
                     PIRNAHSSGFLPEEREWMDRFVASGFIDTFRVFNDQPHQYSWWSFRARAREKNLGWRI
                     DYHFVTENLRPRLKNAWILPHVNHSDHCPVGLEIEI"
     gene            complement(6681..7283)
                     /locus_tag="PLF20_05265"
     CDS             complement(6681..7283)
                     /locus_tag="PLF20_05265"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX80447.1"
                     /translation="MASTEWNKKYNANEYVYGIEPIPYFIEVLERYRPDNLLLPGEGE
                     GRNAVYAAKKGVKVFAFDQSRTAYQKAQKLAKEHGVQIDYKVCDILESNYPRQYFQMI
                     GIFYLHLPVEVRQKAHQHLLNFLAPGGVLVLEAFHKRNIGNDFGPKTVEMLYDEITLR
                     ADFQSLVIEEYFENHYVFSHGCDDHRGQAELIRFCAIKPH"
     gene            complement(8031..8441)
                     /locus_tag="PLF20_05270"
     CDS             complement(8031..8441)
                     /locus_tag="PLF20_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOX80448.1"
                     /translation="MIQQQTIVLPEFPKGIHLITDFIMKQLGTLPDRGLLHLFLLHTS
                     AALAINENYDPTVRQDTDYLFDHLVPEHLPAYKHTLEGADDMPAHFKTIIAGSALTIP
                     ITQGRLHLGTWQGIYMCEFRRHGGSRHLVCTIIE"
     gene            8556..9071
                     /locus_tag="PLF20_05275"
     CDS             8556..9071
                     /locus_tag="PLF20_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HOX80449.1"
                     /translation="MNGTKALTRSRYDKIIGGVAGGLAEYFGMDSAIVRVLFILFTLL
                     YGVGFWVYIVLWIILPYGPLPRRWEQNVPPDFTSNSTPPFSSGGETQINPFPQNQKKL
                     KRDTNMVIGLILIGLGVLFLISQFIPKIHFSHLWPFILIIIGIVLLYNYYQHNKGQSS
                     SENRNKPEQEL"
     gene            9083..10012
                     /locus_tag="PLF20_05280"
     CDS             9083..10012
                     /locus_tag="PLF20_05280"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HOX80450.1"
                     /translation="MEKKFRNVFWGVILLIIGVIFALRNFGIIHFTWRAVFDLWPILL
                     ILWGISILPAKPVLKMILGIFTGAIAITLLLTNPRDESRFYWEGWSYNEHRRYKERNW
                     IPSNQKFVEPYDSAVKTAILKFDAAAGKFTIKDTTNKMVSFESYGNVGPFVMTSYDNE
                     EGRNIKLSLEGVKIKGDIKNDVVIKLNPNPLWKFEIKAGAADMNMDLSSFRVRELNIE
                     GGASLVELKLGHLSDTTNVSLEIGASHSVIYIPRQAGCQVESESVLSSRSLEGFHKLK
                     SGLWQTPDFDESSQKIFIELKAAVSNIEIKRYP"
     gene            complement(10023..11219)
                     /locus_tag="PLF20_05285"
     CDS             complement(10023..11219)
                     /locus_tag="PLF20_05285"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HOX80451.1"
                     /translation="MSRKTFHNELLMTIALFSGFLIFESCHPSSAEQPSPSVIQGFLQ
                     DELQSKIVLHEMDIIEMHPVDSVMTGKDGSFVFSLFITEPVFYQMSIVKNDPLVLIVN
                     PGEKIQLKGKADSLIFADIQGSEESRLMQEYLIHTQRNLRLSESLATLLAVKSQEKDF
                     LTVRDSILKVFDSIYTDQKQFVKNFILHHPGSLASLIIINQKFGRQVVVDEHSNFDLF
                     IKLDSGLLAHYPKSKHTLNYHRQMAERAREREIKKMVRENIEPGHTIPYFRLPSLDEN
                     QWISPSKFKGKKFILAFWIPTQKKSIHQLAQLREFLLASQIKEIPVVAIAFELYRSRW
                     ENVVKAGNMAHWSHASDLKGDASPLFDVFDLMIDSLPVFYLIDEQQTIIGRYSSFEDL
                     KKSLAK"
     gene            complement(11186..11854)
                     /locus_tag="PLF20_05290"
     CDS             complement(11186..11854)
                     /locus_tag="PLF20_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOX80452.1"
                     /translation="MPVNRIQVLNNGNILGLWQITESSEELIPFACLDPDEFVQFSKF
                     KSPLRRLHWLAWRALLRKMIPESNFSLIYDDNGKPFPHDHSFQLSVSHSGNWATCFID
                     ANHPVGLDLEAVHERVLKVKERFLSPLELEYFSPSANDPFVFTVLWSAKEAVFKYIGQ
                     KGIDFRQQITIEPFSPYESHSFFVNIHHNSLAKKLVLKWQNIDSKYLLVHTQCQEKHS
                     IMSC"
BASE COUNT         3589 a         2392 c         2478 g         3704 t
ORIGIN      
        1 ttttcatgaa gtgtatgagg tgcatgatcg gtggctactg catcaatgcg gtcgtcgagt
       61 aaggcctccc atagtgcttt tttatccgca ggagttttta tagaaggatt ccacttgatc
      121 tttgttcctt tttctttata gtctatattg gagaaccaaa gatgatgtat gcatacttcg
      181 gaggtaattt gtttttgatt cgagggaagt ttgttggtaa aaagttttgt ctcttcgacg
      241 gaagaaagat ggagaacatg caggcggcta tcatactccc tggctaatgc gatggctttt
      301 tgagtgcttt tctgacaagc gtcagccgag cggataagag aatggtattc taggggaata
      361 tcttttgcgt agatagattg ataataggca aggtttttac ggatgaggtc ttcgtcttcg
      421 caatgtactg cgataaggca tggagctttt gagaagatat tgattagggt ttcctcgtca
      481 tctatccgca tattacctgt agaagcaccc ataaagactt ttacgccagg tatcatggct
      541 ggatcgatat cgagaatttc gttcagatta tcgttggtag ctcccagata aaaggcataa
      601 ttggcgagtg aactcttaga agctcttttc agtttccatt caagtgtttt atggttagtg
      661 gtttgaggga tagtattggg catatccatg aaagatgtta ctccgccagc tactgctgcc
      721 cttgattcgg tatagatgtc ggctttgtgt gtaagacctg gttctctgaa atgcacatgc
      781 tcgtcgatta ttccgggaag gagatatttt ccctgagctt cgattacaca tgcctttcct
      841 gcgtttgtac cggctctcat gatggaagct atatagggtt cttcgataag aatatccatt
      901 tcctgaatat tcccttcgtt tatcattttg gcacccgtaa tcaacgtttt ttgcattttc
      961 atgggattcc ccgttgaatg aaggtttaaa agtgcaaaga caattattcc tgacgtttat
     1021 tgggaactat ctttttgaat cgaagcttga tgactcccag tgccgcttct ttgaaaattc
     1081 ccctgctcat tttcgattcc cctgccgttc ggtcggtaaa gatgatggga atctctataa
     1141 tcgtaaaacc aagtttccat gcagaatatt tcatttcgat ctgaaaagca tagccggtaa
     1201 atttaatttc gtcgagattg atagtttgca gcacgctgcg actgtagcaa ataaatccgg
     1261 cagtagtatc atggactttt atacccgtaa tcaagcgcac atattttgaa gcaaggtatg
     1321 acatcaatac ccttcctatg ggccaattta ctacgttaac tcccgatata tatcgtgaac
     1381 ctatggcaag atcggcacta ttttcagcaa ctgcttgtcg taaacggatc agatcgttgg
     1441 ggttgtgcga aaagtcggca tccatctcga aaatgaattg gtaatctttt tccaatgccc
     1501 atttgaaacc atggatataa gccgtgccaa gtcctaattt tccacttctt tcttcaatga
     1561 aaagccgttc gggaaattcc tgtatcagtt gctttacaat tctggcggtc ccgtcgggtg
     1621 aattatcatc cacaacaagt atatcgaaag cgggttcgag cgaaaatacc ttgcgtataa
     1681 tccgctcgat gttctccttt tcgttgtatg taggtatgat taccagacta tcggacactt
     1741 gacataaatt atggagcaaa attacggaat tatcttatta tgcgaactat aaataaattc
     1801 ttatcaactt tcttttacaa cttttgtaat tttatattct gctttctttt tgcatcttag
     1861 tgtttcctgt tatatatagt ttgtattatt atccttcgag gaattggacc gatgatgcct
     1921 tgaacgaaac ccataccttc gaacctactt cgagcttcaa ttgaattacc gaagcggtgc
     1981 ttacgatcgc tgctaatgga aagctacagt ctactatcac ttcgaggcct tcacgtgtag
     2041 gaattatttc ctttacaatg ccagggaaat tattgcgtgc cgatgattca agcttttgtt
     2101 ccgacaacac tatatctctg gcgggtataa tgatacttcc atgtgttgag gagattgtgc
     2161 cggtaataaa tatttgcttt tcatcattga tgacagccaa ggaaagtgat gagttatgta
     2221 aagatcgaaa cttgcaatgg aagaaattgc gaattccagc aaattgggct acgaaaggag
     2281 aagctggctt taaaaatatc tctgaaggtt gccccacttg aataatttta ccatcgttaa
     2341 tgatggccat tttactggca agcgtcaaag catcttcata atcgtgactt acatggatga
     2401 tggtaacccc ttgctggttg atagttcgta acaatcttcg cagagctacc ttcagagagg
     2461 tatccacttc acttaggggt tcatcgagta gaagtaagcg tggttgtgaa gccagggttc
     2521 ttgccaaagc aactctacgg gcttctccac ccgaaagctg atgaggataa tggtcgagta
     2581 gcttttctat gctcatggca tgagctaatt tcattatttc atttttcaca taaatgggtt
     2641 cttttttttt cgccctcatg ggaaatgaaa tattttgttt tacagtaaga tgtgggaaaa
     2701 gagccatatc ctgaaaaacc attccaattt tacgtttttg ggttggcaag ctggtaatat
     2761 cgataccatc gaagatgatt ttgccttcat cgggaaatga taaacctgca attagttcga
     2821 gcagttgtga tttacctgca cccgaagggc ctgcaaatac gaagtattca ccttcttcga
     2881 ggcagtagga tggaatttcc agaagaaaat ctcctaatct ttttttaata ttaaaaagtt
     2941 caagcatttt ttgcgggttt agcgagcaat ctcaaaagga tgaagaaaac aaggcttaca
     3001 agaatcaaaa tggcggctaa gggattggca taattgagtc cataattgtt gaacctttca
     3061 aagattaaga caggagcggt catagggtga taagccacga tgattacagc tccgaattcg
     3121 ctcattcctc tggcaaacat catcaccaaa ccggatagta tactacgcca ggctaaaggc
     3181 aatgaaacgg taaaaaatgt tctcacagac gatgcgccca agttcgaagc tgctttctcg
     3241 agacgaattg gaacggcttc gaagcctgaa cgtgccgcat tgatcagaaa aggaaggctt
     3301 acgaaggcca tagcacagga aatagaaatg ggagtgccaa ttagatcgaa tcctgctctg
     3361 gcagcggcag agccaataag agaattacgc gaaatgacgc ctaaaatagc aatgccagcg
     3421 gcagtatgag gaataaccac gggtaaatca attaaaccca gaactactaa tttgcctctg
     3481 aattgcttgc gagcgagtag ataggcgaga ggaattgcgc cgatagctcc tatgaaagta
     3541 gccacaaacg cagtgaacag gcttacggcc aaactggaac ttacctccgg ctcttttatg
     3601 gtattcacta tctgattggg cgaactgcgc aaaaacattc ccagcaaagg agccagaatg
     3661 aaaagtagca ccagcccgcc gagtatggcc atgaccagcg aaaaggtcgt gaaatttctc
     3721 gatttatatg gcattcttgg catgattaat tcataacaaa tgtattgaaa ttggactttg
     3781 cggagaataa aagacttgag atgatatgag ataggcgggg ccgcacagcg gcccgcctat
     3841 acctacatca aaacttcgaa aattctacct tctttccgta tttcctatct atatgcccag
     3901 ttttttcttg atggcagtag gaatagcctc cttatgaacc aatatatcga tggtctttaa
     3961 acgcacaaaa ggtaccgagg catagaaaaa tccattgaat ttattgtctc ccccccatga
     4021 attctttaca taataatatt tatgaccgaa ctgatcgtta ccgtaaccta ctatatgcat
     4081 cccatggtca tcggtggttt gaagattatc gaattctttt tggcgcattt cctgtgtaac
     4141 ttttttttcg ggtacaaccc gatcgagatt atacatctga gaatttcttt ccttttcggt
     4201 taatttttcc catctggcct tttctgcacc acttaactct gtaacatcgg tttcgggtat
     4261 cacggctact cccttgttat aggcaaaccc cttttcgctt acatcggaag cccaacctac
     4321 tgtataacct ttatccagcg cataatcgat aatttccatc aactcgtcaa gaggaacatt
     4381 ataaatctca ccccaggccc agttatcagg cacttcaagt ataaaagtct tgtaaaatgg
     4441 gtgatgtgta aatgaagtga gacaaacgta atcctctgaa tgaagtccaa gcgactcggc
     4501 aaaactcctt gaagtatata gtttgtcttt ataaacgaaa ttttcgggta catgaccaag
     4561 gtatgcatcc atgatccctt taaaaccatt catccaggca gtagatattt tcttgttggg
     4621 attttttatc aaagcatcca tataagcttt tgcaacagca tcgagttcgc catgaacatg
     4681 atttttttct ccataatcga ggccggggta agcttcttct ggcatcaatc ccctgtctcg
     4741 taaagtatga attacatcat ggaattcacc gcctccgcta aaattggtgg ttcctcccat
     4801 acggagatat ttttcggctt ttttttcata ggcatctctt acacaaaaca tttccgaaag
     4861 gtcgtattcg cctttccctt ttcgcaagag ttccgattcg ataaatgaaa tagccgagaa
     4921 actccagcaa gtacccgaac gaaactgatt tttaacggga gtataaggca attgttttac
     4981 gagagtaaac tgataggaac tatcggtttt agtgtcctga gctttaacga aaaatggcat
     5041 gagtaaggca atactcaata accagatgat tgatttttct ttcagtaatt tcatggtcgt
     5101 aaaatttagc aataatgatt tttcgcaaaa atagacactc taactcagca aacaaaaatt
     5161 tatacgatga aattggctgc gaataaagaa attcaaaaac ttttcatata attgtcaaat
     5221 atctattctt tatccctctg agtactcttt ggaattatta tgtaattttg tgatggaaaa
     5281 tatcatgatg ccgaacactc agcaaaatct tttcccatcc tcttcctcag agaggaataa
     5341 aatggaaagc ctgttagggc aaaaaagcgt ggtaatttgg tttacagggc tttcgggtgc
     5401 tggcaaaacg actttgggga ggattttgag agatgagctt ttttcaagag gatttattgc
     5461 ccagatgtta gatggggata atttacgtag cggattgaat aaggatttag gtttcagtgt
     5521 cgaagaccgc atcgaaaata tccggcgagc agccgaagtc agcaaactct tcatggaatc
     5581 aggaatcatt agcataaatt gttttattac tcccaccgaa gccattcgcg ctatggcaag
     5641 aaatattatt gggaaagaaa accttctcga agtttttgtc gattgcccaa tagatatttg
     5701 tcagcaaaga gacaccaaag gattgtatct taaagccaaa gaggggctga tcaatgattt
     5761 tactggagtg aattcaccat ttgaaatccc tcagtattcc gatattcatc tctttacaca
     5821 tatcatgcat ccttatcgat gccttgaagt tttgatggaa tatatcctgc cacgaatcac
     5881 atatcataaa accggcaaat gatttaaatc tctatttcga gacctaccgg gcaatggtcg
     5941 ctatgattca cgtgtggaag aatccatgca tttttcaagc gaggcctgag attttcggta
     6001 acgaaatgat aatcgatgcg ccagccaagg tttttttccc ttgctcgggc tctgaaactc
     6061 caccatgaat attgatgggg ctggtcgttg aaaacccgga aagtatcgat aaaacccgaa
     6121 gccacaaagc gatccatcca ctctctttct tcgggtagga atccactgct atgagcattc
     6181 ctgattggat cgtgaatatc gatgggttta tggcatatat tgaaatctcc cgaaataatc
     6241 agataaggtc tttcttttcg aagattattg acatattgaa ggaaaaattc tagccattgc
     6301 attttgaaat cctgacgtac ttcgccagtt gtacccgaag gatgatagac gctgatgacg
     6361 gagatctccc caaaatcggc tctcagaaaa cgaccttccc gatcatattc ggttttaccc
     6421 attcccttta ccacccgatc gggttctctg agtgaaataa gtccaactcc actataacct
     6481 cttttctcgg cacaatgcca gtaagtatgg tatccgagac gacgaaattc ttcatcaggg
     6541 atttgatcgg gttgagcctt caattcctga agagaaaaaa tatcgggacg ttcctgttcg
     6601 agccattcga gcaacccttt cgataatgcc gaacgtaatc catttacatt gtacgaaatc
     6661 agtttcatag tccttatgtt ctaatgaggt tttattgcac agaaacgaat taactctgct
     6721 tggcctcgat ggtcatcgca gccatgcgaa aatacataat gattttcgaa gtactcttct
     6781 attacaagcg attgaaaatc tgctcttaat gtgatctcat catagagcat ctcaacagtt
     6841 ttgggaccaa aatcattccc aatattccgc ttatggaagg cttctaatac cagaactcca
     6901 ccaggagcca gaaagtttaa taaatgttga tgtgcctttt gccttacttc aaccggcaag
     6961 tgtaaataaa atattccaat catttgaaaa tattgtcgag gatagttcga ttcaagaata
     7021 tcacaaactt tgtaatcaat ctgaacgcca tgctctttcg ccaatttctg ggctttctga
     7081 taagcagttc tgctctggtc gaaagcaaaa acctttactc ctttcttagc ggcgtaaacg
     7141 gcatttcggc cttctccctc gcctggcaat aataaattat caggcctgta tctttccaat
     7201 acttcgataa aatagggaat gggttcaatt ccataaacat attcattggc attatatttc
     7261 ttgttccatt ctgtacttgc cataatcaac aaattaagat tctatatact tccttcgatg
     7321 aatatttcga tacttactca ttttattcga ggcaaaaata caagcctatt ctgattttta
     7381 tcctccccat tcgtcagaaa caattgaata ctaaattttt ttggtttgaa acggaaaaca
     7441 ataacattat gaagaaaacc aatattttgg tcattatgtc atgtgttcgg gtgcaaccac
     7501 aattcatgcc caaaagtcaa taactggagt aaaaggtaga atcgatttga gcggcttaaa
     7561 ttctggacag aagggcgata agagctttaa acttggagtt catgcgtgaa tttgcggtaa
     7621 aattgttttc aatctcttgt tagcgcatca tcccgagcct ttatattcat tgaacggatt
     7681 aaagttagac tatgcaatat tgctatcttt gagggagaaa cccattttta atttgcacta
     7741 tgttgatctt cctctcaaat tagtgttcca tcttacatcg aatttcgaaa tacaggtagg
     7801 gatgtattca gggtgtttac tcgcagcaac tacgagcacc aatgtcagtg tactcgatta
     7861 tttccatatt tcttttgaaa atgaactcga tagaggggcg ttctgtagtt gagattatgg
     7921 aataacaggg ggttaagcca atatttgact gataaatcat taagaattaa aattttctca
     7981 aattgatatc attgagacaa aagaaggcat gcctatgaag agagaccagg ctattctata
     8041 attgtacaaa ctaaatgacg gctgccaccg tgccggcgaa attcacacat ataaattccc
     8101 tgccatgttc ctaagtgaag tcttccttgt gtgataggaa tggtgagagc acttccagcg
     8161 ataatagttt tgaaatgagc gggcatatca tcggcgcctt cgagcgtatg cttataggcc
     8221 ggcaaatgct cgggaacaag atgatcgaat aaataatcag tatcctgtct taccgtagga
     8281 tcatagtttt cattaatagc caaggctgct gaagtgtgaa gtaaaaacaa atgaagcaaa
     8341 ccacgatcgg gcagggttcc cagctgtttc atgataaaat cggtaatcaa gtgtatccct
     8401 tttggaaatt cgggcaatac gatggtttgc tgctggatca tgactaaatt cattaatcaa
     8461 ttaaggtatc atcattggca aaagtaaata ttcagctgta acctgttaag gattgaagcg
     8521 tctatttact tcgaaaacct taaattatca ttattgtgaa cggaacaaaa gctcttacac
     8581 gaagtcgtta cgacaagatt attggagggg tggccggtgg actggccgaa tattttggaa
     8641 tggacagtgc gattgttcgc gtcctgttta tccttttcac tctcttgtat ggtgtaggtt
     8701 tctgggttta tatcgtttta tggatcatat tgccttatgg gcccttacct cgacgctggg
     8761 aacagaacgt tccccctgac ttcacttcca attcaactcc tccattttct tcaggaggag
     8821 aaactcagat caaccctttt cctcaaaatc aaaaaaaatt aaaacgcgat acaaatatgg
     8881 ttatcggcct tattttgatt ggattggggg tactatttct cataagtcaa tttatcccta
     8941 agattcattt tagccatctt tggccattca tccttatcat tattggcatt gtgttgcttt
     9001 ataattacta tcaacacaat aagggacaat catcgagcga aaatcggaat aaacctgaac
     9061 aagaacttta aatttcaaaa aaatggaaaa aaaattcaga aatgtttttt ggggagtcat
     9121 tcttttgatc ataggcgtaa tatttgcttt gcgaaatttt gggattatcc attttacatg
     9181 gcgagctgtg ttcgatttat ggcctatatt gctcatttta tggggtatct cgatactgcc
     9241 ggcgaagccc gtgctaaaga tgatattagg tatattcacc ggagccattg ccattaccct
     9301 cttattaacc aatcctcgag acgaatctcg cttctattgg gaaggatggt cgtataatga
     9361 acatcgtcgt tataaagaaa gaaactggat tccttctaac cagaaatttg ttgaacctta
     9421 cgattctgcc gtaaaaacgg ctattctgaa gttcgatgcg gctgccggta agtttacaat
     9481 taaagacacc accaataaga tggtatcctt cgaaagctat gggaatgttg gaccctttgt
     9541 gatgacttca tacgataatg aagaaggccg taatatcaaa ctttcactcg agggagtaaa
     9601 aataaaggga gatattaaga acgatgttgt tataaaattg aatcctaatc ctttatggaa
     9661 attcgaaatc aaggctggag ctgccgacat gaatatggat ttaagtagct tccgtgtgag
     9721 ggaattgaat attgaaggtg gcgcttcttt agtggaattg aaattaggtc atctttcgga
     9781 taccaccaac gtgagcctcg agataggagc cagccattcg gtgatttata ttccccgcca
     9841 ggctggttgc caggttgaat cggaatctgt gctgtcgagc cgttcactcg agggatttca
     9901 taaactgaag agcggccttt ggcaaacacc cgacttcgac gaatcttccc agaaaatctt
     9961 tatcgaactc aaagccgcag tttccaatat cgaaatcaaa aggtacccct gatggattat
    10021 aatcatttag ccaacgactt ttttaaatct tcgaacgagg aatatctgcc tataatggtt
    10081 tgctgctcgt cgatcagata aaaaaccggc aaggaatcta tcatcaaatc aaatacatcg
    10141 aacaagggag aagcatctcc cttcaaatcg gaagcatgac tccaatgagc catgtttccg
    10201 gcttttacca cattttccca tcttgacctg taaagttcaa acgcaatggc aacaacagga
    10261 atttctttga tctgcgaagc aagcaaaaac tctctgagtt gggcgagctg atgaatggat
    10321 tttttttgag taggaatcca gaaagccagt ataaattttt tccccttgaa tttggaagga
    10381 ctgatccact ggttttcgtc gagcgaagga agacgaaaat aaggaatcgt atggcctggt
    10441 tcaatgtttt ctcgaaccat ttttttaatt tccctttctc gagctctttc agccatctgc
    10501 cgatgataat tcaatgtgtg tttgcttttt ggataatgag ctaataaacc tgaatcgagc
    10561 tttatgaata aatcaaaatt ggagtgttcg tctaccacaa cctggcggcc aaatttttga
    10621 ttgataatga ttagcgaagc cagtgagccg ggatgatgca gaatgaagtt ttttacgaat
    10681 tgtttttgat cggtatatat tgaatcaaac actttgagga tggaatcacg taccgttaga
    10741 aaatccttct cttggctttt tactgccagc aatgtagcta atgattccga taatcgtaaa
    10801 ttccgttgtg tatgaataag gtattcctgc ataagcctgc tttcttccga accctgtata
    10861 tcggcaaaaa tcaaactgtc ggctttgcct ttcaattgaa ttttctcacc aggattcaca
    10921 atcagaacaa gaggatcgtt tttcacaata ctcatctgat aaaaaacagg ctcggtaatg
    10981 aataaggaga acacaaaact gccatctttc cctgtcatca cggaatcgac aggatgcatt
    11041 tcgataatat ccatttcatg aagcacgatt ttggactgca attcatcttg gagaaatcct
    11101 tgaattaccg aaggggatgg ttgctcagcc gagcttggat ggcaggattc aaaaatcaga
    11161 aaacccgaga atagggctat agtcatcaac agctcattat ggaatgtttt tcttgacatt
    11221 gggtatgaac taagagatat ttgctatcta tgttttgcca ttttaaaacg agcttctttg
    11281 ccaatgaatt gtgatggata tttacgaaga atgaatgaga ttcgtatgga gaaaagggtt
    11341 cgatagtgat ttgttgtcgg aaatcgatac ctttttgtcc gatatatttg aagacagctt
    11401 ctttagccga ccataaaaca gtaaatacga aggggtcatt ggctgatggc gagaaatatt
    11461 cgagttctaa tggtgataag aagcgttctt tcactttgag cactctttca tgaacagcct
    11521 cgaggtcgag cccaacagga tggttggcat ctataaagca ggtagcccaa ttcccggaat
    11581 gacttaccga aagttgaaaa gaatgatcat gggggaaggg tttcccattg tcatcataaa
    11641 ttaaagaaaa attggattcg ggaatcatct tgcgaagtaa ggcccgccat gccagccagt
    11701 gaagcctgcg taagggtgat ttgaatttac tgaattgcac gaactcatca ggatctaaac
    11761 aagcaaacgg aatcagttcc tctgatgatt ctgtgatttg ccataaaccc agaatgtttc
    11821 cattattaag tacttgaatc ctgtttactg gcattgctgc ataatttcat gaatcgactc
    11881 tgcaaaagta atacaaggca tttatttgtg aagaataaca ttttactaat tactaattgg
    11941 ttaattttca tgatttcata ttagatggag aatgtgtaat aaaaagttta tacctaattt
    12001 tgctgattca acagtatttt cagaaaactt tatttgtatc atgtttcaaa atagtcaaac
    12061 aaccttaaat ttcgaatata agataaaaga cctttcgctt gccgattggg gtcgtaagga
    12121 aatagaaatt ggaaagcatg tagatgacaa agttcccgaa gaa
//
LOCUS       DAOORU010000060        12055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_6558_length_12055_cov_5.127250, whole genome shotgun sequence.
ACCESSION   DAOORU010000060 DAOORU010000000
VERSION     DAOORU010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12055
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6558_length_12055_cov_5.127250"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            474..2315
                     /locus_tag="PLF20_05295"
     CDS             474..2315
                     /locus_tag="PLF20_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80453.1"
                     /translation="MKKLFTITFILLIGLQAWSTARVIPSKEIRQKSVKIKHQRPIKN
                     IDNIYGMLENPGLKSIVAYPEADLGVTQYDLQTNSTLGNRFYMYPEGQMVHVWTIGMT
                     PTSYPDRGTGYNYWDGTSWQPAPTSRIESSRTGWPGYAPAGANGEAVIAHRSSDLLFS
                     YRENRFSGSWIQTPIAGPIGTEPAWPRMIASGPDNQYLHYIVNSYTEYNGQGMALLYS
                     RSLDGGQTWEIQNAIIEELGPEYYYEIGADAYVMASRGETVAYLIGRPWMDLVLMKSN
                     NNGETWHKTVIWQHPYPFYDFTYVTDTFYCVDNSIALTIDKDYMVHVAFGINKVQGNS
                     DGTYYLYPYVDGIGYWNESRPPFSDNKMALCPYGFEGSEMIENYNLIGWSQDLNGNGQ
                     LDFQSDLLYYRSIGLSTMPSIYVDDQDNVYVAYASTTEGYDNGTNNFKHIWMRVASNK
                     GELWSGKFIDIMSDISHIFDEGIWPQLGQLSDDNVYLVYNVDPHPGLALDGDHDPVDN
                     HQLFVSIPKNQLGVGIHDIELTNISKPYPNPASDKVFVSVDQKAAANVSVELTNLAGQ
                     IVKRCDYGNKTAGTHLLSINTSSLKSGYYLLTVYSGTQTVTYPVLIQ"
     gene            2697..3182
                     /locus_tag="PLF20_05300"
     CDS             2697..3182
                     /locus_tag="PLF20_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR04366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbuC family cupin fold metalloprotein"
                     /protein_id="HOX80454.1"
                     /translation="MSLIAITNPHIEELIRAAQKSKRGRIIFNYHTYAADPVQRMLNA
                     IEPGSYVQPHKHENPDKSEVFIILKGRIAVIIYDDTGEIDDFIILDREEGHFGAEVPP
                     RTWHSIIALESGTVIYEVKHGPYDPTTDKVFAPWAPAENHPQAGEFLLQVTKKLNRFV
                     E"
     gene            3204..3623
                     /gene="mscL"
                     /locus_tag="PLF20_05305"
     CDS             3204..3623
                     /gene="mscL"
                     /locus_tag="PLF20_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOX80455.1"
                     /translation="MMSLLQEFKTFAMRGNVIDMAVGIILGAAFGKIVSSLVNDVIMP
                     PIGLLIGGINFTDIKITLKSAAYNGSNQLIPAVTLNIGSFIQTLVDFIIIAFAIFMMI
                     KAMNQFHKKKEKPASVLAPSNEEKLLTEIRDILKTKK"
     gene            complement(3765..4814)
                     /locus_tag="PLF20_05310"
     CDS             complement(3765..4814)
                     /locus_tag="PLF20_05310"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOX80456.1"
                     /translation="MRQEEEIGKFFKGKFENYAETPPKRVWENIASYLGHPKPVKNIF
                     HQRFFIITITLVGIATLLSIVFLTRKSNQTLQNDFVQNTTTISTSTENKITQQAQTFA
                     FDSSNNKPDRKNEIPASIHSSALLTSKPFPSQESSIVINENRNLPVKSISEAIEAQPE
                     LPIQPEITEPLEIEPAMDFAESLQIQSIETEILNYQVISVCKGEEVFLNAGDGQNYKW
                     NNGAQTQAITFQALENSLFDVNFVNPDGKKVKQTFDIRLLNCSIYIPKAFSPNNDGFN
                     DIYKVEAEGLAEFEMKIFSQWGELVFQSKDIHSGWDGRIHGSRAPSGIYIYQIRYTDP
                     ISHKRAFFGTLTLLP"
     gene            complement(4811..5362)
                     /locus_tag="PLF20_05315"
     CDS             complement(4811..5362)
                     /locus_tag="PLF20_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX80457.1"
                     /translation="MKDETLLIKGCLEGNAKMQKCLYDTYASKMFGICLRYANSHAEA
                     SDILQEGFIKVFTKLQDFRNEGSLEGWIKRIMINTAINFCIKEKKHFTTDLDNTGEII
                     EQAAMNYDSLHLKDLMRLVQKLPSGYRTVFNLYEIEGYTHKEISELMGISINTSKTQL
                     MHARRFLQKQLLSIEKQTNSIKR"
     gene            complement(5467..8280)
                     /locus_tag="PLF20_05320"
     CDS             complement(5467..8280)
                     /locus_tag="PLF20_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80458.1"
                     /translation="MHSNHCIPACFFMIFSFFLHENSEAQFFYSGEDPSYIQWQQIES
                     PQNRVVFPKGEEELGRKCSQALKEVLSNKYYTRNLKLSRLDVLLHTRTLTSNAWVSWA
                     PARMEIFTTPPQDLSTISWHNYLALHESRHFVQINSLNTGFTRVLSYLFGQQAHGLVL
                     GLHLPLWFIEGDAVWAESVFSHSGRLRDPFFFLQQNAQWSAGIHYSYDKSYFGSYRDF
                     VPDRYVLGARLISNANLKYGDDIFYKGLDKAAHQPWRPRAFTHGFKKETGITPSQLLR
                     NSQMKEYAHYSNEYIKSSIFKISEYTNYIPTGFIGNSIICIQQKLGEIDRLIEINSES
                     EQKVIFIPGYYLPESVSTCDSITIMIVKQPDGRWQHKSSSNIGIYKHDKEKYSEIKTH
                     NLLFSPDVSLDKTKMALVEIKENGISVLNVIDLGTNQIMSTIDAPIDETIECPRWQVN
                     NQQIVFWLAGDQGKALAMGDIQLKSIQQLTNYTFNPVYRLFGKGDTIFFSGAYQQKPQ
                     IMAYSISSQKTFCLTQAQYGADYAVWDSHRKRLVYADYTLDGYKLTIVPSDSLLWEEV
                     RGYWMSDNQHSLPCMDYTYTFSNNEDTNSTRDQITPYKKSSHLFNFHSYGPVSISPDD
                     ATINPGFTLMSQNLLNTSILRGGYEYLIQEETNQWFLNYQYRGFYPIFGLDAFYKTNN
                     HSINHNELPQDHNEIYPAQVLEMKANLTFPWHWLKRLWQQNISFRMGLSSIWVDEYKD
                     GPSLRLDDSFFTMNFEFFWSRIFKSTSQHIYPQWGQTFYGLLRLAPMENIDRLQGLAA
                     EASFYTPACGKHDGFKFYVGFENQNLKHYVFSHSISMPRGYTNLGYGSIFSSQFSWRT
                     PLMYPDLNFSSLFYCKRIYGGLNGDLAYWNEKWYRSIGIELTGEFYILRLPMPVSFTI
                     RSSYLPQQKSWRVDVILRADFSF"
     gene            complement(8604..9329)
                     /locus_tag="PLF20_05325"
     CDS             complement(8604..9329)
                     /locus_tag="PLF20_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOX80459.1"
                     /translation="MAKFIDLSQRILYEDNHLLVINKLPGEIVQGDKTGDKPLVEIAK
                     DYIAQKYDKPGNVFLGVVHRLDRPVSGAIILARTSKALERLNAMLQERKIMKTYWAIV
                     KNKPPKENDTLVNYVWRNKQKNKTIVFDHPIKKSSEASLTYRIVAQSVNYYLLEIGLH
                     TGRHHQIRAQLAHIGCPIKGDLKYGFPRSNDSAFIHLHSLKLILIHPVKNIPLELIAP
                     PPHDALWNYFFELFYPDLSHKNL"
     gene            complement(9350..10174)
                     /gene="panB"
                     /locus_tag="PLF20_05330"
     CDS             complement(9350..10174)
                     /gene="panB"
                     /locus_tag="PLF20_05330"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOX80460.1"
                     /translation="MNESFGSQRYTKITTHVLQEMKLKGEKIAMLTAYDYSMAGILDA
                     AGIDVILVGDSASNVMAGYTTTLPITLDEMIYHASSVVRAVQRALVVVDLPFGTYQGN
                     SKMALASAIRIMKESGANAIKIEGGKEIIESVERILSAGIPVMGHLGLTPQSIHKFGT
                     YVVRAKEETEANKLKEDAQLLEKAGCFAIVLEKIPAALGAEVTQLVKCPIIGIGAGSG
                     VDGQVLVLHDMLGITIKFSPRFLRRYHNLYEEIKGAIQTYIEDVKNLDFPNAREQY"
     gene            10950..>12055
                     /locus_tag="PLF20_05335"
     CDS             10950..>12055
                     /locus_tag="PLF20_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOX80461.1"
                     /translation="MKRLSLLFTVLICSLYGFAQILSLKQVQKVAQTWFQQRLPSDFP
                     EFSIKETLPVINEGDTLFYAVNFYPKGFVMISASKKLHPIPAYSFESWYTSENQPENA
                     KAWIEQYIHQAVYAHRTSSIPNQEAARQWERLLDLEQGSKIKFSGKSVEPLTCADWNQ
                     DWPYNEMCPVDPAGPGGHCYAGCVSTAIGLIMYYYRWPDTGVGYYSYTQSPYGLLEAD
                     FGNTHYAWEKMTNSINESDSAIAQLLYHIGVSCDLVYGPYGSGMYNHKAANAFRTHFK
                     YSPETQYVYRDSTTMDWDSLLTTHLDRKMPLYYAGWSVPDINGHAFVCDGYQGTGYYH
                     FNFGWRSSFNGYYYTNNLTPGDSNFNLAQEIIIN"
BASE COUNT         3676 a         2260 c         2316 g         3803 t
ORIGIN      
        1 aaaaatttaa atatttaata atcagtaaca taacatttaa aatcatagca aaaaccctat
       61 tttcccttgt tttttcaggg taaaatgctc aagttgggtt aagatacgta attatcaatt
      121 atcgagaaac atgagcttga gggaagttct ccttgttctc atccgaaatc ccttgatgga
      181 atagaggatg agaacttaga aaaagtaatg tctgacgttg aaacatttcc acaaccatca
      241 tcggtataac aatcactgtc ggttgttcat tgtcgatgca aagttcttta attttttaaa
      301 ttttgtaaaa ctttctatac gattgtttga ataataggaa gttaagccag ttagctaaat
      361 ggaataagaa taatttatat gtaatatctc taaagattaa tttcagactt tgtactttat
      421 aagagatttt ttacttttgt tggcaaacat tcaacccact aaaaaattca accatgaaaa
      481 aactctttac tatcacgttc atcttactca ttggcctgca ggcatggtca accgccaggg
      541 ttattccctc gaaagaaatc aggcaaaagt cagtaaaaat caaacaccag cgtcctatta
      601 aaaatattga caacatatat gggatgctag agaatcctgg tttaaaaagt atagtggctt
      661 acccggaagc cgatttaggt gttactcaat atgatcttca aactaactcc actttgggca
      721 atcgctttta catgtatccc gaaggtcaga tggttcatgt ttggactata ggaatgactc
      781 caaccagtta tcccgacaga ggaactggtt ataattattg ggatggtact tcatggcagc
      841 cagcacctac gagtcgtatc gaaagttcgc gtacaggctg gccaggatat gcaccagcag
      901 gagccaacgg agaagccgtg attgcccatc gatcatccga tcttcttttt tcttatcgag
      961 aaaaccgttt ttcgggctcg tggattcaga ctcccatagc tggccctata ggaaccgaac
     1021 ctgcctggcc acgtatgatt gcttctggcc ctgataacca gtatctccac tatatagtaa
     1081 atagctatac cgaatataac ggtcagggca tggctttgct ttattctcgt tcattggatg
     1141 gggggcaaac ctgggaaatc caaaatgcta tcattgaaga attaggccct gaatattatt
     1201 acgaaatagg agccgatgca tacgtaatgg catctcgggg agaaaccgta gcatatctca
     1261 ttgggagacc atggatggac ctcgttctta tgaaatcaaa caataatggc gaaacctggc
     1321 acaaaaccgt catctggcaa catccctatc cattctatga ttttacctat gtcactgata
     1381 ccttctattg tgtggacaac tccattgcct tgactatcga taaggactac atggtacatg
     1441 tagccttcgg aatcaataaa gttcaaggaa atagtgatgg aacctattat ttatatcctt
     1501 acgtagacgg tatcggatac tggaatgaat ctcgccctcc cttttctgat aacaaaatgg
     1561 ctctttgccc ttatggcttc gaaggttcgg aaatgataga aaattacaat cttatcggct
     1621 ggagtcagga tttgaatggt aatggacaat tagattttca gagtgatcta ttatattata
     1681 ggtctattgg actttctact atgccaagca tttacgtgga tgatcaggat aatgtttatg
     1741 tggcttatgc ctctactacc gaaggttatg ataatggaac caataatttc aaacatatct
     1801 ggatgagggt agcatctaat aaaggtgaat tatggtcagg caaatttatc gatattatga
     1861 gcgacatttc gcatatcttc gacgaaggaa tctggcctca gttagggcaa ctctccgacg
     1921 ataatgtgta tttggtttac aatgttgacc cgcatccagg tcttgctctt gatggagatc
     1981 acgatccggt tgataatcac caattgtttg tatctattcc taaaaatcag ctcggtgtgg
     2041 gcatccatga tatagaactg actaacattt cgaaacctta ccccaatcct gcttccgata
     2101 aagtcttcgt atctgtcgac caaaaggctg cagctaatgt ttcagtagaa cttaccaacc
     2161 ttgccggtca aatcgtaaaa cgttgtgatt acggaaataa aactgccgga actcatttgc
     2221 tctccattaa tacatcttct ttaaaatcag ggtattattt attgacagtt tattcgggta
     2281 ctcaaacggt tacttatcct gttcttattc agtaaaccat atcaagaggc ggttattctc
     2341 gatgaaattc tttcatcata gaatactctg accgaattca gcttttaaca aaaccctgtc
     2401 agagcaaagg ttatcaaaat tcaccaattt tgggattctt tgacagggtt tttttaatga
     2461 atttcaaaaa agacacccta ccaacgaaat gaacaagaaa attgaatata tgataaattt
     2521 ttcaagcact ttgaacgatt ttcttgttaa tggcttttcg ataaactgag cgtaatggca
     2581 actgcaatga gactatccta aaaattcgat aagaaaatta tttcgcaata ccttacttta
     2641 aattgtttta tcgcatattc catctacttc ctttaatttt gaaaattcat tccattatga
     2701 gtttaatagc aattaccaac ccacatatcg aagagcttat aagagcagca caaaaaagca
     2761 aacgtggacg tataatattc aattatcaca cttatgctgc agatcctgtg caacggatgt
     2821 tgaatgccat agaaccaggt tcgtatgtac aacctcataa acacgaaaac ccagataagt
     2881 cggaagtttt cattatttta aaagggagga tagctgtaat tatctatgat gatacgggtg
     2941 aaatcgatga ttttatcatt cttgaccggg aggaaggaca ctttggagcc gaagttcctc
     3001 ctcgcacatg gcattccatt atcgccctcg agtctgggac ggtaatctat gaagtaaaac
     3061 acggtcctta cgatccaact accgacaagg tatttgctcc ctgggctcct gcagagaacc
     3121 atccacaagc tggtgaattt ttattgcagg taaccaaaaa attgaatagg tttgtcgaat
     3181 aaatttctaa taataactta tcgattatga gcctgttaca agaattcaaa acctttgcca
     3241 tgcgtggcaa tgtaatagac atggccgttg gaattatcct cggtgcagct ttcgggaaaa
     3301 ttgtttcttc acttgtaaat gacgtaatta tgccacctat tggtcttcta atcggaggga
     3361 taaattttac cgatatcaaa atcaccctta agtcagccgc ttacaatgga agcaatcaat
     3421 taattcctgc cgtaacattg aatatcggca gcttcattca aacgctggtt gatttcatta
     3481 ttattgcttt tgccattttt atgatgatca aggcgatgaa ccaatttcat aaaaagaaag
     3541 aaaaacctgc ctccgttctt gctccttcta atgaagaaaa actactaacc gaaatccgcg
     3601 atatactaaa aacaaaaaaa taatacctaa aaattaatat atcattaaaa atggtcatcg
     3661 ataaaatttt caaaatactt cctatgtgca gaatagattg aaaggctgcg agcatcactg
     3721 ctggcaacct ttctctgatc tcagtaaata ggtatttttg ggctttaagg aagtaatgtc
     3781 agtgtaccga agaacgccct tttatgtgaa atgggatcag tatatcgtat ttggtaaatg
     3841 tatatacccg aaggggcacg actgccatga atccggccgt cccacccaga atgaatgtcc
     3901 ttagactgga agaccaattc accccattgc gagaatatct tcatttcaaa ttctgctaaa
     3961 ccttcggctt ctaccttgta aatgtcatta aaaccatcgt tattaggaga aaaagcctta
     4021 ggaatgtata tagaacaatt aagtaagcga atgtcgaaag tttgttttac ctttttcccg
     4081 tcgggattga cgaaatttac gtcgaagaga ctattttcga gtgcctgaaa agtaatagct
     4141 tgagtttgtg ctccattgtt ccatttataa ttctgaccgt ccccggcatt cagaaatact
     4201 tcctctcctt tgcatacgga aatcacttgg taattaagga tttcggtttc gatagattgt
     4261 atctgaaggc tttcagcaaa atccatagcg ggttcgattt ctaaaggttc tgtgatttca
     4321 ggctgtatag gaagttcggg ttgtgcttct atagcctcgg agatagactt cactgggaga
     4381 ttacggtttt catttattac aatcgaagat tcttgagagg gaaaaggttt gcttgtcagt
     4441 aatgcgcttg aatgtataga tgctggaatt tcgttttttc gatcaggttt attattcgag
     4501 gaatcaaagg caaatgtttg ggcttgctgc gtaattttat tttcagttga agtactaatc
     4561 gttgttgtat tctgtacgaa atcattttgt aatgtttggt tcgattttcg agtcagaaac
     4621 acaatggaga gcaatgtagc aatgccaact aatgtaatgg ttataataaa gaaacgttga
     4681 tggaaaatat tcttcactgg cttaggatga ccaagatacg aagcgatatt ctcccaaacc
     4741 cttttaggag gagtttcggc ataattttca aattttccct tgaaaaactt tcctatttct
     4801 tcttcttgtc tcatcgttta atcgaattgg tttgtttttc gatacttaaa agttgtttct
     4861 gaaggaagcg gcgggcatgc attagctgag tcttcgaagt attgatagaa atacccatca
     4921 attctgatat ttctttatgt gtatagcctt cgatttcgta aaggttaaaa acggtacggt
     4981 atcctgaggg taatttctga acaagtctca taaggtcttt caaatgcagg ctatcataat
     5041 tcatcgcagc ctgctcgata atttcaccgg tattgtccaa atcggtcgta aagtgttttt
     5101 tttcttttat acaaaaattg atagctgtat tgatcataat tcttttaatc catccttcta
     5161 aggaaccttc attacgaaaa tcctgtagtt tagtaaaaac cttgataaaa ccttcttgta
     5221 gaatatcgga ggcctctgcg tggctgtttg catatcttaa acatatccca aacatcttcg
     5281 atgcataagt atcataaagg cacttctgca tctttgcatt gccttccaaa catcctttaa
     5341 tcagaagagt ttcatctttc atgaaagttt cctgtttaat tgagacataa aaattaaaaa
     5401 gaaggttgta tgaataatta attttttaga atatgttata aggaggtttt tttgtttcct
     5461 gaaatttcaa aagctgaaat cggcccttag aatgacatcc acccgccaag atttttgttg
     5521 aggtaaatag ctcgatcgaa tagtaaaaga tacaggcatg ggcaacctca aaatataaaa
     5581 ttctcctgtc aattctatgc ctatgcttct ataccatttt tcattccaat aagcaaggtc
     5641 gccattaagg ccaccatata ttcttttaca ataaaataat gaactaaaat tcaaatcggg
     5701 atacatcaat ggagttcgcc aagaaaactg tgatgaaaat atagatccat aacccaaatt
     5761 agtgtatcct cttggcattg aaatagaatg cgaaaaaaca taatgtttta aattctggtt
     5821 ttcgaatccg acataaaatt taaatccgtc gtgctttcca catgcagggg tataaaatga
     5881 ggcttcggcg gctaagcctt gcagtctgtc aatattttcc atcggagcca gacgcagtag
     5941 accgtaaaaa gtctgtcccc attgtggata aatatgttgt gaggttgatt tgaaaatcct
     6001 tgaccagaaa aattcaaaat tcatagtgaa gaaagaatca tcgagcctca gtgaagggcc
     6061 atctttatat tcatctaccc aaatggaaga gagacccatg cgaaatgaaa tgttttgttg
     6121 ccaaagtctt ttcaaccaat gccacgggaa agtaagatta gccttcattt ctaacacctg
     6181 agccggataa atttcattat gatcctgagg tagctcattg tggttgatac tatgattatt
     6241 tgtcttataa aatgcatcga gtccaaaaat cggataaaaa cctctgtatt gataattcaa
     6301 gaaccattga ttggtttctt cttggatgag atattcatag ccacctcgta gtatagaagt
     6361 atttaggagg ttttgcgaca taagggtgaa acctggattt atggtggcat catcaggtga
     6421 aatggagact ggaccataac tatgaaaatt aaatagatgc gaagattttt tatatggtgt
     6481 aatctgatcc cgagttgagt ttgtatcttc attattagaa aaagtgtagg tgtagtccat
     6541 gcatggaagg ctgtgctgat tatcactcat ccagtatcct ctaacttctt cccatagtaa
     6601 agaatcggaa ggaacaattg tgagtttata gccatcgagg gtgtaatcgg catagactaa
     6661 acgttttcta tgagaatccc atacagcgta atcagcgcca tattgggctt gtgtaagaca
     6721 gaaagttttt tgggaactaa ttgaataggc cataatttgt ggtttttgct ggtaagcccc
     6781 cgaaaaaaag atagtatctc cctttccaaa aagcctgtaa acagggttaa aggtatagtt
     6841 cgtgagttgt tggattgatt taagttggat atctcccatt gcaagagcct taccttgatc
     6901 tccagccaac cagaaaacta tctgctggtt attcacttgc caacgtgggc attctattgt
     6961 ttcgtctatt ggagcatcaa tagtactcat tatctgattt gtgcccaaat caatgacatt
     7021 taaaacagaa attccattct ctttaatttc tactaatgcc attttggttt tgtcgagcga
     7081 cacatcaggc gaaaaaagaa ggttatgggt ttttatttcg gaatattttt ccttgtcgtg
     7141 cttgtaaatt ccaatatttg aggaactctt atgctgccac cgcccatcgg gttgcttcac
     7201 tatcattatt gtaattgaat cgcaggtact tactgattca ggcaggtaat aacccggaat
     7261 aaatataacc ttttgctcac tctcggaatt tatctcgatc aatcgatcga tttcacccaa
     7321 cttttgctgg atgcaaatta tactgtttcc tatgaaaccg gtaggaatgt agttggtata
     7381 ttcagagatt ttaaagatgg atgattttat atattcgttg ctataatgag cgtattcttt
     7441 catttgggaa tttcttagaa gttgtgaagg ggttattcca gtctcttttt tgaatccatg
     7501 tgtaaaagcc cgtggtcgcc atggttgatg ggcggctttg tcaaggcctt tgtaaaagat
     7561 atcgtcaccg tattttaaat ttgcgttcga aatgaggcga gcacccaaaa cataacgatc
     7621 gggaacaaag tcgcgatatg agccaaaata agatttatca taggaataat ggattcctgc
     7681 gctccattga gcattttgct gaagaaagaa aaaaggatct ctcaatcggc cggaatgaga
     7741 aaatacactt tcggcccaaa cagcatcacc ttctataaac cataatggca aatgcaagcc
     7801 gagtacaagg ccatgagctt gttgcccaaa taaatacgat aagaccctgg tgaaacctgt
     7861 attcaaactg ttaatctgaa caaaatgtct ggattcgtgt aaagctaaat aattatgcca
     7921 tgaaatagtg gacaaatcct gaggaggtgt ggtaaatatc tccatacggg caggagccca
     7981 tgaaacccac gcattcgaag tgagtgttcg agtatgtaat aaaacatcga gccgggataa
     8041 cttcaaattt cgagtataat atttgttgct cagcacttct ttcaatgcct ggctgcattt
     8101 tcttcctaat tcttcttcac ctttgggaaa tactacccga ttctgaggtg attcgatttg
     8161 ctgccattgg atatatgatg ggtcttctcc actataaaaa aactgagctt cagaattttc
     8221 atgaagaaaa aacgaaaata tcatgaaaaa acaggcagga atgcaatgat ttgaatgcat
     8281 aggcaagaaa ttaacagcga aactacctaa tattctataa tcccagatta tttcttcttc
     8341 ccctgaaaaa tgggaatgtc gcaaatcctg ctgaaagatt ccttcctcat tcgagaatgc
     8401 ctcatggttg tatttaaaaa agtttacatg gtggtatttg gttccgagtg aattatgtaa
     8461 tatttctaat attgggtact ttttatttat acggatatcg tttttatgat tttataaatt
     8521 aatcaagatg agttggttca tgaatttgct ttttcgataa tgagtgttgg atggaaaagg
     8581 tttataaaag agatataaaa aaattacagg tttttatggg ataagtcagg atagaacaat
     8641 tcaaaaaaat aattccaaag agcatcatga ggcggaggtg cgatcagctc aaggggtatg
     8701 ttttttaccg gatgaatcaa aatgagtttg agactatgca aatggataaa agcactgtca
     8761 ttgcttcttg ggaaaccata tttgaggtcg cctttgatgg ggcaacctat atgagctaat
     8821 tgggctctga tttgatgatg gcggccagta tggagcccta tttcgagaag ataataattg
     8881 acactttgtg ccacaatacg ataagttaga gatgcttcag aacttttttt aataggatga
     8941 tcgaatacga tggttttgtt tttctgttta tttctccaaa catagttgac gagtgtatca
     9001 ttttcttttg gaggtttatt tttaacaata gcccaatacg ttttcataat tttcctttcc
     9061 tgaagcatag cattgagccg ttcgagggct ttgcttgtgc gggccaaaat gatagctcca
     9121 cttacgggac ggtcgagtct atgaacaacc ccaagaaata catttcctgg cttatcgtat
     9181 ttctgggcta tataatcttt ggcaatttca acgaggggtt tatctcctgt tttatctccc
     9241 tgtacgattt cgccaggaag tttgttgata acaagcaggt ggttatcttc atataagata
     9301 cgttgcgaaa gatcgataaa tttcgccata gatgaaaatc cttgcaaact cagtattgtt
     9361 cacgggcatt gggaaagtcg aggtttttca cgtcttctat ataggtttga atggctcctt
     9421 ttatttcttc atacaagttg tggtagcgac gcagaaaacg aggagaaaat ttgatagtaa
     9481 tacccaacat atcatgcaaa accaatacct gtccatccac gccagaacca gcaccaatgc
     9541 caataatcgg gcattttacc aattgagtaa cttcggcacc caaagcagca ggaatttttt
     9601 cgagcacgat agcaaagcaa cccgcttttt cgagcaattg ggcatcttct ttcagtttgt
     9661 tggcttcggt ttcttctttg gccctaacta cataagtacc aaacttatga atagattgag
     9721 gcgtcaatcc taaatggccc atcacgggaa tgccagccga aagtattctt tccacacttt
     9781 ctatgatttc tttgcctccc tcaatcttaa tggcattggc accactttcc ttcatgatgc
     9841 gaatggccga agccaaagcc atcttagaat ttccctgata agtaccaaaa ggcaaatcta
     9901 caacgactaa tgctctttgt acggcacgca ctaccgagct ggcatgataa atcatttcgt
     9961 caagcgtaat aggaagagta gttgtataac ctgccattac attggaagca ctatcgccaa
    10021 ccagaatcac atctatcccg gcagcatcga gaattccagc catcgaataa tcataggccg
    10081 taagcatggc tatcttctcg ccctttagtt tcatctcctg aagtacgtga gtggtgatct
    10141 tcgtatatcg ctgtgaacca aaactttcat tcataatcag attctttgaa aaatttatgc
    10201 aaaattatta aaaataattg ccttgattta aatgttttga aattaagtta tgcttgtggg
    10261 tatgttctta ttccttatcg caaaatttac agttaataat aaaaatctaa cagtttcagg
    10321 ctcgtatggt aaaagcgaaa aaagccaaaa tctatgaata ataagtcatt ccaaaaatag
    10381 tgtctgcaag ccatttttaa acagtggatt ttcgaaaaat tgcttgtaaa gccagacagt
    10441 cggtcataaa ttcaaagttt atattgctcg gtttcccctc acgttggaga ggggctgcaa
    10501 taatgtaagc atggagatat tcttcgaagt tgggtaaatc ttctttgttt tgagcaatac
    10561 tttacaaact attgaagagt tccattgttt ttttgcccct ttttgttaaa ataattgtag
    10621 ttaaaggtca ttatccgaag gtgtccttcg ttggttaaaa ttggtcatgc gcgaaaaagt
    10681 cataaatttg ttgttgaaaa ggatagaatt aatattggga ttaatttaca aactatgctg
    10741 gatgatgatt tttgtttttt caataatttt taataattta agaagaatat tatatatttt
    10801 gaatcaataa aatatttata tgaatatttt ggtaagatgt ttatatagag tctgtctata
    10861 ggcacgtatg tccagatatt atttccttcc actgcacctg tctctcattt tttgtatttt
    10921 tgttactcga aaatcctaat ttctaactca tgaaacgttt gtctttactt tttaccgtgc
    10981 ttatctgctc actttatgga tttgcccaaa ttctttctct aaaacaagtg caaaaggttg
    11041 ctcaaacatg gtttcaacaa agactgccca gtgattttcc agaattttcg atcaaagaaa
    11101 cacttcccgt cattaatgaa ggcgatacct tattttatgc cgttaatttc tatccaaagg
    11161 gatttgtcat gataagtgct tctaaaaaac tgcatcccat acctgcctat agtttcgagt
    11221 catggtacac gtcagaaaac caacctgaga atgcaaaagc ctggatcgaa caatatattc
    11281 atcaggcagt gtatgcccat cggacgagct ccattcctaa tcaggaagct gctcggcaat
    11341 gggaaaggct cttagattta gagcaaggat cgaaaatcaa gttcagtgga aaatcggtcg
    11401 agcctcttac ttgtgctgat tggaaccaag attggcctta caacgagatg tgcccggtcg
    11461 atcccgccgg acccggcgga cattgttatg caggatgtgt atcaacagcc ataggtttaa
    11521 ttatgtatta ctatcgctgg cctgatactg gcgtaggtta ttattcttac actcaatcac
    11581 cttatggtct cctggaagcc gattttggta atacccatta cgcatgggaa aagatgacca
    11641 atagtataaa tgaatccgat tcggccattg cacaattgct ctatcatatc ggtgtttcgt
    11701 gtgatctggt ttatggacct tatggctcgg gaatgtataa tcacaaggca gctaacgcct
    11761 ttcgaacaca ttttaaatat tctcctgaaa cacaatacgt ttatagagac agcaccacta
    11821 tggattggga cagtttgtta accacgcatc tcgaccgaaa gatgccctta tattatgcag
    11881 gctggtcggt gcccgatatc aatggtcatg cttttgtgtg tgatggttat cagggaactg
    11941 gatattacca ttttaatttt ggttggagaa gctcattcaa tggctactat tataccaata
    12001 accttacacc cggagacagc aacttcaatt tagctcagga aatcatcatc aattg
//
LOCUS       DAOORU010000061        11966 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_6628_length_11966_cov_7.281504, whole genome shotgun sequence.
ACCESSION   DAOORU010000061 DAOORU010000000
VERSION     DAOORU010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11966
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6628_length_11966_cov_7.281504"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(475..2757)
                     /locus_tag="PLF20_05340"
     CDS             complement(475..2757)
                     /locus_tag="PLF20_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOX80462.1"
                     /translation="MASKENFFRKYFTNQGKYPKEMDFYIKRMWQIYAGLIAVILLFF
                     ILLSAGVFGEMPSFEDLENPKSNLASQVISADGKVLGTYYIQNRSNIHFNELSPYLIQ
                     ALIATEDARFEKHAGIDYKALLRVSAGVLTGRYAGGGSTITQQLAKNLFPRGQNLSTP
                     AFVLKKFKEWVTAVKLERNYTKEEILAMYLNTVDFGSNAYGVKSAANTFFSKEPRELN
                     AEESALLIAIVNAPTRYSPVRNPENALKRRNLVLNQMAKHDYLSLHQADSIAQLPIDM
                     SKYRLMDHKSGLATYFREILREELTDWCSKHYKSDGTPYNLYKDGLRIYTTINSHMQE
                     YAEYAVRKHLSEELQPAFFRQMKGQKNAPFSNDLTKEEINKIYEDAMKRSDRYIHMRQ
                     AGISKDSIRKAFQTKIPMKVFSWKGDIDTVMSPMDSIRYSFGFLHAGLMSMETHTGFV
                     KAYVGGIDYRYFKYDHVKMSKRQVGSTFKPFLYTLAMQEGEFNPCSMVPNVPVTFELY
                     DGTLWTPKNSDDAREGQMVPLKWALANSVNYISAFLIKRYSPEAVIKIAQKMGITSEI
                     PPVPSICLGTPEISLYEMTSAFNTFANQGVWVEPIMLTRIEDKSGNVLESFVPRKEEA
                     ISQETAYLMVELMKGVVQSGTGVRLRYKYGFDNPIAGKTGTTQNQSDGWFIGLVPQLT
                     TGVWVGGELRSIRFRNISMGQGASMALPIWAYYMKRVYADPSLGIRKDDFERPSSVPP
                     FNFNCPAIDEDAAVYQQDEF"
     gene            complement(2789..3262)
                     /locus_tag="PLF20_05345"
     CDS             complement(2789..3262)
                     /locus_tag="PLF20_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HOX80463.1"
                     /translation="MRIYSFLCLSLFSILMISCQTNDVFNGMEKIPNAKWNNNHQVTF
                     QTFIQDTLVPLDVYVQLRNNQYYPYSNIYLFLTTISPDRTTYRDTLECILASPEGQWL
                     GKRTGTVFESRFLIKHNVLFARSGPYVFKISHGMRESEITGIIGVGFRISKPKTN"
     gene            complement(3286..4389)
                     /gene="ricT"
                     /locus_tag="PLF20_05350"
     CDS             complement(3286..4389)
                     /gene="ricT"
                     /locus_tag="PLF20_05350"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOX80464.1"
                     /translation="MTKKIDINNPLFSRGCTCLPTYAEQNVYSNGCAKFSTDDYLSEI
                     PPSGYETDFEFAEIRLKNNHKEFMHVPSELKLHQGDIVVVDGNPGHDIGIISLTGHTA
                     RLQMKAKNIDPERTELKKVFRLARPADIEKWIAVSSREDKTMLQTRGIVEDLNLSMKV
                     NDVEYQGDGSKAIFYYTADDRVDFRELIKILADRFKIRVEMRQIGIRQEASKVGGIGP
                     CGRELCCSIWINRFHTVSTQAAKIQQLAPNPQKLTGQCGKLKCCLNYEYEAYMDALKE
                     FPDMELVLKTQKGEAVCQKIDVFKKLMWYAYVKDPNNFLAIPLDKVNEIEEMNLSGKN
                     PENLEDYALAEEPKSFYEQARDYDLLDESGMDE"
     gene            complement(4478..5692)
                     /locus_tag="PLF20_05355"
     CDS             complement(4478..5692)
                     /locus_tag="PLF20_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80465.1"
                     /translation="MYFRDIPGMDVLKGKLIESVNNKRISHTQLFFGPEGSASLGLAI
                     AYGRYIQCRQPIHNDACGKCPSCLKYNKLQHPDLHFIFPVAKKGKSSDTEEDEIVGKT
                     GDEPSSLDFMREWREFILSNKALVSYKEWLDFAGLGKRALIYTRDTREIFKRLNYKSY
                     ESEYKILIIWYPEKFFHSASHRLLKALEEPPEKTVFILVSENKDEILPTILSRCQSTF
                     VPPFTQNELKQALQSNLHLPEDQALHYASVADGNFIVARQLANENSDFHNYFDFFANW
                     FRYCYNLKYKPKKSEAKKAPYFVDALKIFESLGQEGQKQFLRFGIQLLRNSLFFSMDT
                     SQLVKLEGEEQLFARNFSPFIHSRNIALYSDAFGKAIMALERNSSPNMVFTNLTMSIH
                     QLITLAKAKSVS"
     gene            complement(6008..7402)
                     /locus_tag="PLF20_05360"
     CDS             complement(6008..7402)
                     /locus_tag="PLF20_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HOX80466.1"
                     /translation="MLKSQIYSSRRKALRELMGNGIAFLPGNTEAPINYPANTYHFRQ
                     DSNFLYFFGLNQADLVGMIDLDKGIDILFGDEPSIDDIIWMGTQPSMKERASLVDVSD
                     TRSLSQLSTYLSDAIRAGRKIHYVPPYRAETAHWISVLLGIKPMFLKSYASLELTKAI
                     IQLREIKKEEEIEEIEKMIGVARLMHTTAMRMAKPGIIERQIFGRMEGIAFENANGTS
                     FPTILSINGQTLHNHYYGNVLTEGRLIVSDAGCESEDGYCSDITRSTPVGGKFNSRQK
                     EIYEIVLNANLKAIETAKPNIFYRDVHWEASRIIAAGLKELGLMKGDVDEAVATGAHA
                     LFFPHGLGHQMGLDVHDMEGLGEDLVGYDAEISRSIQFGLGLLRMAKRLKPGHIVTDE
                     PGIYFIPALIQQWKAQNKFDEFIDYQKVETYLDFGGIRIEDDILITENGCRVLGPFIP
                     KTVKEVEEEAAKSF"
     gene            complement(8316..9200)
                     /locus_tag="PLF20_05365"
     CDS             complement(8316..9200)
                     /locus_tag="PLF20_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014775862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOX80467.1"
                     /translation="MKQAVALVLSSGGARGLAHIGVIEELEQAGFEIKSVAGTSMGAL
                     VGGIFATGKLKECTEWICSLDKKSVLSMFDFTISSTGLVKGQKVLDQIQQRIPDLPIE
                     DLLVHFCAVATDIKNQKENVFEKGSLFEAIRASISIPGVFIPYKSDGKILVDGGVVNP
                     LPLNHVKRPENSILVAVDVNAAHALHHSKRKKIRESQNNHELKFLELLRTKSKNLKLQ
                     KNEEEQYNYYQLLTLSAQMMLQRITNLTLAIYPPDMLIRIPMDSFGTFHFYKAKEIIE
                     SGRNAAKKALEEYRKNHC"
     gene            complement(9470..11887)
                     /locus_tag="PLF20_05370"
     CDS             complement(9470..11887)
                     /locus_tag="PLF20_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOX80468.1"
                     /translation="MIYPENFEKKIGFTEIRVLIENECISSMGRDEVKSMEFETDCGQ
                     IEMLLKQVGEMMLILQYEDYFPSQDFFDLRSELIRIKPEGTYILMEVLPDLRSSVFTL
                     QQILDFFTRTKEGTYPYLRKLSSKVVLEKSIHQKLNLLLTEKGELRDDASEKLMKIRQ
                     QIRQKENLAVQRIHEILVEAKKSKLLPSDTEITIRNSRMVLPIPSSGKRQLHGFVHDV
                     SASGQTIFVEPEEVFEINNDIIELKASEKKEIIAILTAFADFLRPHLDDLIEAYTFMG
                     VIDFNRAKARFSISIHSSIPKLQPYSLIQWLGAYHPLLLLKNHAQKKPVVPMDLILNE
                     DQRILIISGPNAGGKSVCLRTAGLLQYMLQCGIPIPVKETSTAGIFWDIFLDIGDEQS
                     IENDLSSYSSHLLNMKFFLKNATSQTLFLIDEMGEGTEPRVGGAIAEAILEKLSQQKA
                     FGVITTHYSNLKSLEGKVEGIVNGAMLFDIHRMQPLYRLQIGKPGSSFAFEIAHILGF
                     PEEVMQLAAEKTGETQLSFDHQILRIEDEKNQLEQKKAELQLADELLTRALKRYQDLY
                     NQLDQSKKEILKKAREEAAELIRQSNRLIEKTIEEIKKSDAERKTTQKLRETLKEFQK
                     VLEEESKELEPRPIHLVKQEINDNKESTNETKPLQVGDWVRIKNLDASGELFSLNGQD
                     AVVIINSVKLNIRADRLEPASKPVFEKKIRGHLLEDINQKLQDFRLTIDVRGNRAEDA
                     LAQVNHYIDEAVLLGIKEIRILHGKGNGILRNIIREHLNQHQSVSAFSDETPEKGGSG
                     ITLVKIK"
BASE COUNT         3395 a         2421 c         2330 g         3820 t
ORIGIN      
        1 agaagtttat acttttactg aaaattttga tgactgtatg gacagactct aattatgttc
       61 tattcattca ttcgatacag tggattgttg tatctcaaaa taagattttg caatgaaagt
      121 atgagggaaa tttcctatgg aagatcttga aaccgaaatt ccataagcat tttagaaaac
      181 ttaattgctt tcagagctaa aaaacatgat agaaattcat aaagctttag cttttaaagg
      241 tttttttggc ctcagccaat atgggtttca atgaaatatt aagatattac aaataattga
      301 tatttaaaat tttattggta agtttacgat tacttatatt aggatttcca tggatcttta
      361 aataaaaagg tttgaatgaa atttctggtt ccattttttt aaattcttgt ctctggagca
      421 tattttttct ccatttttaa acaaggaata aaaaaatttt ctaattcgac ttattcagaa
      481 ttcatcttgt tgataaactg cggcatcttc gtcgatagcg gggcagttga agttgaaggg
      541 agggactgat gaagggcgtt cgaaatcgtc tttacggata ccgagagaag gatcggcata
      601 gacgcgtttc atataataag cccagatagg caaagccatg cttgcgcctt gtcccataga
      661 aatgttacgg aagcggatgc tgcgtaattc tccacctacc caaacgcccg tagtgagttg
      721 aggaacgagg ccaataaacc atccgtcgct ttggttttgt gtggttccag ttttcccggc
      781 aatgggatta tcgaagccat atttataacg caggcgaact cccgttccac tctgtacgac
      841 acctttcatc aattccacca ttagataggc agtttcctgg ctaatagctt cttctttacg
      901 gggcacgaaa ctttcgagca catttccgct cttgtcttca atgcgcgtaa gcatgatggg
      961 ttctacccaa actccctgat tggcaaaagt gttgaaagca cttgtcattt cgtaaagaga
     1021 aatttcgggt gttcccaggc agatggaagg aactggagga atttcggaag taatccccat
     1081 tttttgtgct attttgatta ccgcttcggg gctgtaacgt tttatcagaa aagccgaaat
     1141 ataatttacg gaattagcca gagcccattt tagaggaacc atttgtcctt cgcgtgcgtc
     1201 gtcactattt ttaggcgtcc agagggtccc gtcatacagt tcgaaagtta ccggaacatt
     1261 gggcaccatc gagcagggat taaattcgcc ttcctgcatg gccaaggtat aaagaaaggg
     1321 cttaaacgta gatcctacct gtcgtttcga catttttaca tgatcgtact tgaaataacg
     1381 ataatctata cctcccacat aggctttcac aaatcctgta tgggtttcca tgctcatcaa
     1441 tccggcatgc agaaaaccaa acgaatagcg aatgctgtcc attgggctca tcaccgtatc
     1501 tatatcgcct ttccagctaa aaactttcat gggaattttt gtttggaagg cttttcggat
     1561 agaatctttc gagatgcctg cctggcgcat gtgtatgtaa cgatcggagc gtttcatggc
     1621 atcttcgtaa attttgttaa tttcttcctt tgtcagatca ttcgaaaaag gtgcattttt
     1681 ttgtcctttc atttgtcgaa agaaggccgg ttgcagttct tcactcaggt gtttcctgac
     1741 ggcatattcg gcatattcct gcatgtgaga attgatggtg gtatagatgc gcaagccatc
     1801 tttatagagg ttgtaaggtg tgccatcgct tttgtaatgt ttcgagcacc agtcggtaag
     1861 ttcttcccgc aatatttctc tgaaataggt agccagtccg cttttatggt ccatcaatcg
     1921 gtatttcgac atatcgatgg gcaattgagc gatggaatcg gcttgatgta aactaaggta
     1981 atcatgttta gccatttgat taagaacgag gttacggcgt ttgagggcat tttcgggatt
     2041 tcggacaggg ctgtaacgag taggggcatt cacgattgcg atcaataagg ccgattcttc
     2101 tgcattcagt tcacgaggtt ccttcgagaa gaaggtattg gcggccgatt ttacgccata
     2161 agcgttgctt ccgaaatcca cggtatttaa atacatagca aggatctctt cttttgtgta
     2221 gttacgctcg agcttaacgg ctgtaaccca ttccttgaat tttttcagaa caaatgctgg
     2281 ggtagagagg ttttgtccgc gagggaaaag attcttagcc aattgttgtg taatggtgct
     2341 tcctcctccg gcatagcgtc ctgtaagaac tcctgcacta acccttagaa gggctttata
     2401 gtcaatcccg gcatgttttt cgaaacgagc atcttcagta gctataagtg cttgaatgag
     2461 atagggcgat aattcattga aatggatatt tgaacggttt tgtatataat aagttcccaa
     2521 aactttcccg tcggctgaaa ttacctgaga agccagattg cttttaggat tttcgaggtc
     2581 ttcaaacgag ggcatctccc caaaaacacc ggctgaaagt aaaataaaaa aaagcagaat
     2641 cactgcaatc aagcctgcat atatctgcca cattcgttta atgtaaaaat ccatttcttt
     2701 tggatatttc ccttgattgg taaaatattt cctaaaaaaa ttttctttcg aagccattga
     2761 aacatcaatt aaaaatgata aatgatattt agttggtttt aggctttgat attcggaatc
     2821 ccactccgat gattccggtt atttctgact cacgcatgcc atgagaaatt ttgaaaacat
     2881 aaggtccgct tcgtgcgaaa agtacattat gcttgatgag aaaacggctt tcgaaaaccg
     2941 ttcctgttct tttgcccagc cattgtcctt cggggctagc aagtatgcat tccaaggtat
     3001 cacggtaggt tgtccgatcg ggggaaatgg tagttaaaaa aagataaatg ttggaatagg
     3061 gataatattg attattgcgc aactgcacat aaacatcgag cggaacgaga gtatcctgaa
     3121 taaaggtttg gaaggtgacc tgatgattat tgttccattt agcattagga attttttcca
     3181 tgccgttaaa aacgtcattt gtttgacatg atatcatcaa aatgctaaaa aggctcaggc
     3241 ataaaaatga ataaattctc atctgggggg ttgtcttatt aaatattatt catccatacc
     3301 agattcatcg aggaggtcat aatctctggc ttgttcgtag aaggattttg gttcttcggc
     3361 caatgcataa tcttcgagat tttctggatt ttttcccgag agattcattt cttctatttc
     3421 gtttactttg tcgaggggta tggcgaggaa gttattgggg tctttcacgt aagcatacca
     3481 cattagtttt ttaaagacat cgattttctg gcaaacagcc tcaccttttt gggttttcag
     3541 aacgagttcc atatcgggaa attccttcaa ggcatccata taagcctcat attcataatt
     3601 caggcagcat ttgagctttc cacactggcc agtaagtttt tgaggattgg gtgcaagttg
     3661 ttgaatttta gccgcctggg ttgacacggt atggaaacga tttatccaga tagagcagca
     3721 aagttcacgg ccacaagggc caatgccacc cactttgctg gcttcctgcc ttatacctat
     3781 ctggcgcatt tctaccctaa tcttgaaacg atcggcaaga atttttatga gttcacggaa
     3841 atcgactcta tcatcggctg tataataaaa tatggcttta ctcccatcac cctgatattc
     3901 tacatcattt accttcatcg agagattcag atcctcgact atgcctcgtg tctgtaacat
     3961 agttttatct tcacgtgagc tcactgctat ccatttctcg atatcggccg gcctggccaa
     4021 acgaaatact tttttcaatt ccgtacgttc aggatctata tttttagctt tcatctgcag
     4081 acgagccgta tggcctgtaa gactaattat tccgatatca tggccaggat tgccatctac
     4141 caccactata tcgccctgat gaagctttag ttcggatgga acatgcataa attctttgtg
     4201 attatttttc aagcgaatct cggcaaattc gaaatctgtt tcatatcccg aagggggtat
     4261 ttcggaaaga taatcgtcgg tcgaaaactt tgcgcaacca ttagaataaa cgttttgctc
     4321 ggcataggtt ggaaggcaag tgcaacctct cgaaaataat ggattgttta tatcaatttt
     4381 tttagtcatc ggcaaacaag gctattacga aatcatttca aaaaatagat ttacacattt
     4441 gaaggcgcaa agaaatattt tacaaaatta cataaaacta actcaccgat tttgcctttg
     4501 ccaaagttat gagctgatga atgctcatag tgagattggt aaaaaccata tttggactgc
     4561 tgtttcgttc aagagccatg atggcttttc caaaagcatc ggagtataaa gcaatgtttc
     4621 gagaatgaat gaaaggcgaa aaattacggg caaacaattg ttcctcaccc tcgagtttga
     4681 cgagttgact ggtatccatg ctaaagaaca agctattgcg taacaattga attccaaagc
     4741 gcagaaattg tttttgcccc tcttggccca aggattcgaa gatttttaag gcatctacga
     4801 aataaggagc tttttttgct tccgattttt taggtttgta tttgagattg tagcaatagc
     4861 ggaaccaatt ggcgaaaaaa tcgaaatagt tatggaaatc gctgttttcg ttggcaagct
     4921 gccgtgcgac tatgaagttg ccatctgcca ccgaggcata atgcagggct tggtcttcgg
     4981 gtagatgaag attgctctgc aacgcctgtt tgagctcatt ctgagtaaat ggagggacaa
     5041 aagtggactg acagcgcgaa agaatagtgg gaaggatttc atctttattc tcggaaacca
     5101 aaataaaaac tgttttctcg ggaggctctt cgagtgcctt gagcaggcgg tgagaagcag
     5161 agtgaaaaaa tttctcggga taccagataa tcaatatctt gtattctgat tcgtaactct
     5221 tgtaattaag ccgtttgaat atctcccttg tatcacgtgt ataaatcagt gcacgctttc
     5281 ctaagcctgc aaaatcgagc cattccttat aacttacaag tgccttgttg gagaggataa
     5341 attctctcca ttccctcata aaatccaggc ttgaaggctc atctccagtt ttcccgacga
     5401 tttcatcttc ttctgtatcg gatgatttgc cctttttggc taccggaaag ataaaatgca
     5461 aatcgggatg ctgtaattta ttgtatttca aacaggaggg gcatttgccg catgcgtcat
     5521 tgtggatagg ttgcctgcat tgaatatatc tgccataagc tatggccagt cccagacttg
     5581 ccgaaccttc aggcccgaag aaaagttggg tatggcttat acgtttattg tttacacttt
     5641 cgatgagctt tcccttgagc acatccattc ccggaatatc cctgaaatac attcgatttt
     5701 ttaatatacg atttcaaggc aaagatattc caaactgatt tatccccaca ttctttcttc
     5761 tttaactcaa caaatctgac ttttggcaat tgagtgctct gatgagagta tttagtgtgc
     5821 gtttttattt tatcactacg ccctcggaat actcatattc cgattcataa agattttttt
     5881 gtggattgta atcgacaaaa gaaacatgaa atattaatgg agtttattgg cgctatccaa
     5941 ggctttttaa ttttacccta tgatgaaaaa atcgtaagct cctaaaatcc tattcgttct
     6001 atgtctctca aaacgatttt gctgcctctt cttccacttc ttttaccgtc ttgggaatga
     6061 aagggcctaa aacccgacaa ccattttcgg taattaagat atcgtcttca atgcgaatgc
     6121 ctccaaaatc gagataagtt tcaacctttt ggtagtcaat aaattcgtcg aacttatttt
     6181 gagccttcca ttgctgaatg agtgctggaa taaagtaaat tccaggctca tcggtaacta
     6241 tatggccagg tttcaaacgt tttgccattc gtagtaaacc caatccaaat tgaatgctac
     6301 ggctaatctc cgcatcgtat cccaccaggt cttctcctaa accttccatg tcatgtacat
     6361 cgagacccat ctgatgacca aggccatgcg gaaaaaacaa agcatgagca ccagttgcta
     6421 cggcctcatc cacatctcct ttcatcaaac ccaattcctt taggcctgct gcaatgattc
     6481 gagatgcttc ccaatgaaca tcacggtaaa aaatattggg ttttgctgtt tctatggctt
     6541 ttaaattagc attcaacaca atctcataaa tctccttttg tcggctgttg aactttccac
     6601 caactggagt agaacgggta atatcactgc aataaccatc ttccgattca catccggcat
     6661 cggaaacaat caagcgacct tcggtaagaa cattgccgta ataatgatta tgaagcgttt
     6721 gtccattgat ggataaaatg gtaggaaagc tcgtcccatt agcattctcg aaagcaatgc
     6781 cttccattct cccaaagatc tgcctttcga tgatgccagg cttggccatg cgcatagccg
     6841 tcgtatgcat caagcgggca acgcctatca tcttttcgat ttcttcaatc tcttcttctt
     6901 tctttatttc gcgcagttgg atgatagcct tcgttagctc caatgaagca taagacttta
     6961 ggaacattgg tttgatgccc aatagaacgg aaatccagtg agcagtctcg gcacgataag
     7021 gaggaacata atgaattttc cttcctgctc gtatagcatc cgatagatat gtagacaatt
     7081 ggctcagaga gcgtgtatca gaaacatcta ccagagaagc tctttccttc atcgaaggct
     7141 gggtccccat ccatataata tcgtcaatac tgggttcatc gccaaaaaga atatctattc
     7201 ctttgtcgag atcgatcatc cccacaaggt cggcctgatt caagccaaaa aaatagagga
     7261 agttactatc ctgcctgaaa tgatacgtat tggcgggata atttattggt gcttcagtat
     7321 tgcccggaag gaatgctatc ccattcccca tcaattcacg caaagccttg cgacgactgg
     7381 aataaatctg tgattttaac atagaactat ggtttttacg atttatagaa agattatttg
     7441 agcctgcatt atttaaaact tattttcaca agtaaaattg atgacgctaa attaaatctt
     7501 tctctcaaag taagatttct gtttggcaaa ttatccaaat actgacgacc ttaagttaaa
     7561 aaacaccacc ttctatcgcc aatggtatga cgtcatccta tatttcttat tcagcagcgt
     7621 tagtaatcca tcgaatgatt tagtcatcga gaggcgaagt atatggcggc aacatcttta
     7681 ataaacggcc attttcatcg ataaggcatt tcttaaaatt ctattttgct tccaaatcca
     7741 tgaccccatt gtctgattta ccattcaacc agcaatagat tgaatgcatg ttctctccct
     7801 ttgatcaaaa tcctccacat catcgtaaaa ttacaccatc attattagta gtacaaaaat
     7861 tcctaatctc actttcggtt ccagattctt gatttaagaa gttattgact gggaaagcga
     7921 ctatttggaa gttcttatcg tggtaattat cgtggaattg ttgaagcttc tcgtattgtg
     7981 gagtaagtcc gcatttactc tttaccttta cagggtaaaa cgaataaaat cttaaaatct
     8041 tcaaataaac gcatggcatt atttatcaat aattttctat aaatttacaa taaaaaataa
     8101 taccaatagc agttttaatt gattaattat caaaaagtta ttaacaattt tcagtttata
     8161 gacagactct aattaaaagc aaatcagtag ttctgagatt ttttattcaa tctttagcta
     8221 aggaggctat tttgaattag ccacataaac ttacatctgt ggaagacaag atttcggttt
     8281 tgacccatct tctttttatt cgattttttt gtcggtcagc agtgattctt tctgtattct
     8341 tccaaggcct tttttgcggc atttcttcct gattcaataa tttcttttgc cttgtagaaa
     8401 tgaaaggttc caaatgaatc cattgggatc cgaataagca tatcgggagg ataaatcgcc
     8461 agagttagat tggtaattct ctgaagcatc atctgagccg aaagggtaag taattgataa
     8521 taattatatt gttcttcttc atttttttga agtttgagat ttttcgattt tgttcttaaa
     8581 agttcaagaa atttcagttc atgattattt tggctttctc gaattttttt ccgcttcgaa
     8641 tgatgtaaag catgcgcagc gtttacatca acggcaacca gaatcgaatt ctcgggtcgt
     8701 ttcacatggt ttagcggcaa aggattaaca actccaccat ccacaagtat tttcccatcc
     8761 gatttgtaag ggataaacac acccggaatg gaaatggaag ctctaatggc ttcaaaaagg
     8821 cttcctttct caaaaacatt ttctttttga tttttaatat cggtggccac agcacaaaaa
     8881 tgtaccagta aatcttctat gggcaaatcg ggaatccttt gctgaatttg gtcgaggact
     8941 ttttgacctt ttaccagtcc cgtcgagctt atcgtaaagt cgaacatact caaaaccgat
     9001 tttttatcga gcgaacaaat ccattctgta cattctttaa gtttgcccgt ggcaaaaatt
     9061 ccccccacca gtgcacccat cgaagtgccc gcaaccgact ttatctcaaa acctgcctgc
     9121 tcgagttcct ctatgactcc tatatgagcc aatcctcttg ctcctccgct cgataatacc
     9181 aatgccactg cttgtttcat tccccgaaaa ataaagttct ttctttctat ttattatgtg
     9241 acttcctaac tcttgaaaat tattgaagct tattagaaac gacaaagtta tataagattc
     9301 gcacagtggt aattcataaa tagatatatg gtatgggaat caagagctgc aaaaaaagtc
     9361 caatttcaac cgctgatatg gatcaagagg ttctatatta gtcacagata caaatatcct
     9421 gaaataaaaa tttgataaag aacattcttc taatttcaaa aagagcagtt tatttaattt
     9481 tcaccagggt aattccactg ccgccttttt cgggtgtttc gtcagaaaaa gcagataccg
     9541 actgatgttg gtttagatgt tcccggatga tatttcttaa tatcccattg cccttgccat
     9601 gcaatattct aatttctttg attccgagaa gcacggcttc atcaatataa tgattcacct
     9661 gagccaaagc gtcttcggca cgattccctc taacatcgat ggtcaatcga aagtcttgca
     9721 gtttttggtt gatatcttcc aacagatggc cacgtatctt tttttcgaaa actggtttcg
     9781 aagcaggttc aagacgatcg gcacggatat tgagttttac tgaattaata attacaacag
     9841 catcctgacc attcaacgaa aagagttccc ccgaggcatc caggtttttg atgcgtaccc
     9901 aatcaccgac ttgtaatggc tttgtttcat tcgtagattc tttattgtca tttatttctt
     9961 gtttaacaag atggataggt cgaggctcaa gttcttttga ttcttcttcc agaactttct
    10021 ggaattcttt gagtgtttct cttaattttt gcgtagtttt cctttcggcg tccgattttt
    10081 taatttcctc gatggttttc tctattaaac ggttcgattg tctaattagt tctgcggctt
    10141 cttctctggc ttttttaagg atttcctttt tcgactggtc taattgatta tacaaatcct
    10201 gataacgttt aagggctcga gtcagtaatt catcggcaag ttgaagttcg gctttctttt
    10261 gttcgagttg atttttctca tcttctattc gtaaaatctg atgatcgaat gaaagctgag
    10321 tttctccggt cttttcggct gccaattgca tgacttcttc aggaaacccc agaatatggg
    10381 caatctcgaa ggcgaacgag gaacccggtt tgccaatctg taatcgataa agaggttgca
    10441 ttctatgaat gtcgaaaagc attgcgccgt tgactatgcc ttcaactttc ccttcaagcg
    10501 atttcaaatt ggaataatgt gtggtaataa cgccaaaagc cttttgctgg ctgagttttt
    10561 ccaatatggc ttcggcaatg gcccctccta ctcggggttc agtcccttcg cccatttcat
    10621 caatcaagaa tagagtttgt gaagtagcat ttttaaggaa aaacttcata ttcaataaat
    10681 gagagctata gctgctgaga tcattttcga tggattgttc gtctccgatg tcgaggaaga
    10741 tatcccaaaa aattcctgct gttgaagttt cttttacggg tatgggaatg ccgcactgaa
    10801 gcatatattg aagtaagccc gccgtccgaa gacataccga ttttccgcct gcattagggc
    10861 ccgaaatgat aagaatccgc tggtcctcat tcaggataag gtccatagga actactggtt
    10921 ttttttgagc atgattctta agtagaagca aaggatgata agctccgagc cactgtatca
    10981 atgaataagg ttggagcttg ggaatagagg aatggataga aatcgagaaa cgggctttgg
    11041 ccctgttaaa atcaataact cccataaatg tatatgcttc tattaaatca tcgagatgag
    11101 gacgcaaaaa atcggcaaag gcagtcaaaa tggcgatgat ttcctttttt tcggaagctt
    11161 tcaactcgat aatatcgtta ttgatttcga aaacttcctc gggttcgaca aagatagttt
    11221 gcccgcttgc cgaaacgtcg tgcacaaaac catgcaattg cctttttccc gaagaaggga
    11281 tgggtaatac catacggctg ttacgaatag tgatttcggt atcggaagga agaagtttgc
    11341 tttttttggc ttcgacaagg atttcgtgta tccgttgaac ggccagattt tccttttgcc
    11401 ttatttgttg tcttattttc attagttttt ctgaggcatc atcgcgcaat tcacctttct
    11461 cggtgagcaa aagattcaat ttttgatgaa tggatttttc gagtacaact ttactactca
    11521 gttttctcaa atagggataa gttccttctt ttgttcgagt aaaaaaatct aaaatctgct
    11581 gaagagtgaa caccgaagaa cgcaggtcgg gtaaaacctc catcaaaata taggttcctt
    11641 ccggctttat acgtatcaac tccgatcgta aatcaaaaaa atcctgagaa gggaaataat
    11701 cctcgtattg gagtatcagc atcatctcac caacttgctt caaaagcatt tcgatttgtc
    11761 cgcagtcggt ctcgaattcc atgcttttta cttcatctct gcccatactg ctgatacact
    11821 cattttcgat gagcacccta atctcggtaa aaccaatctt cttttcgaaa ttttcaggat
    11881 aaatcaatgg ttcagttcat tcaatcgaaa atacaaattt acatgaaaac tttcgattga
    11941 cacacagacg aataaaaatc cagtcc
//
LOCUS       DAOORU010000062        11782 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_6763_length_11782_cov_5.468150, whole genome shotgun sequence.
ACCESSION   DAOORU010000062 DAOORU010000000
VERSION     DAOORU010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11782
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6763_length_11782_cov_5.468150"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..586
                     /locus_tag="PLF20_05375"
     CDS             <1..586
                     /locus_tag="PLF20_05375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80469.1"
                     /translation="RFNSGATENFDMDYDSYFLAGFAPNFYSVAGDEHFALNTLPVLT
                     EGLSIPLGFVKNASSGFNIELQETMEGITIYLTDLKTNTNQNLSKSPVYTFTAEEGDL
                     PDRFKLTFGNVGFSEPSISPFSIYSGNGAIYINNGNQIVEGTVTIYSITGQAITTRSL
                     SGDRLQKLSFNGKPGCYIVRVTTDKGVYSQKIIL"
     gene            590..856
                     /locus_tag="PLF20_05380"
     CDS             590..856
                     /locus_tag="PLF20_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80470.1"
                     /translation="MKKIIRNITLILFLLFGSATINNVLADDPAPPPLPGEHGMGGNK
                     APEGGGAPIGSGLGILLGLGLAYGATKVIRKNSEKVISKNSEEE"
     gene            complement(1088..2926)
                     /locus_tag="PLF20_05385"
     CDS             complement(1088..2926)
                     /locus_tag="PLF20_05385"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOX80471.1"
                     /translation="MIKIRIRWMLVFLVVVAGKFCTAQELAYDAIYNYIIKEYTLSKE
                     GSVDYHYSSSMKLLTHQAFQRYFGESFIVYNPQFQRLKVNKSRTIMADGKKVDSPLNA
                     LNEVLPRFASGSAAYNHLREMVVSHTGLEVGATIEFDYNLVSQPTGMPYLMGEEILSF
                     PVPVESYKVIVRVPEGTILNYKMLNLRLSPEVSFAKGMMIYTWNFGKVAANPHEIYMP
                     EDSYYSPYLSFSTSLSLQRSLELLSNQPAFTDTKLPHTAMARIDQIKASDASDSKKII
                     QIRNLVINELSYLKIPSSVIAFKFRTPAEVWESLSGTEIEKTILLASMLRQAGYQADI
                     VAAVPTNVLDTKWFNPLVIDAYYVRTEIPGSASVYLSAINEHPYNLLPDLYGNTLLLL
                     DPAAESVKKWEIYPENSTLKVKGNFEVKSASVEGNGELELTGRYHPFYRILENDKEIT
                     NILTGFCSGENISSFKSKQSNLNRLQTEISVKADQTLTQLAKGFYEMELPFARTGVTS
                     WNVASMPSSRISPFAIPYFLIEDYDYTLQIPDTLELLTPVRNLEIRRDFGAVRIQLSK
                     NGNMVKIRRTIEFVENEVNPMKYGELREIFIEWMDPQYRKLVFKKK"
     gene            complement(3279..6302)
                     /locus_tag="PLF20_05390"
     CDS             complement(3279..6302)
                     /locus_tag="PLF20_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX80472.1"
                     /translation="MAGKNFQFFKAFPEDPISKAWDKEELIYSLAYGEGKWVLITDPS
                     SPYSGQRWSTRTEFPKDVISTGWNDGLDISWIGYGEGVWFVVMSGNTGIEDQRWITNG
                     DFPSGEIKEAIKAGESVLQLVYGNDRWAAVFGKTEHYGQQIFGLYSDFPAKTIKEYWS
                     KGYYITSISPCKGKWGLVMTKDAPFENQFYVSDKKFPEAEVKSKIAEGYEITDIGYGN
                     GIWLVVMSVPIETRIPGEEEEFTYDPDEAEPSQRAAPQKEIEVNPKAIRYFQTGMEYF
                     NRKEYQKAISYFEDALKITPEFPSALNGIGAAWSWLGDMDKALKYYEDSYRLDHSNPV
                     VFSNLVYTLNDMGKTEEIYQTALECPSATLKEVEDAMPLYHAGKALLEKGKAKRALEY
                     FKLASKLEPDESLFKDAIAEAEANPEDKPKPSSSPASQPETNKSLDELLAEVNKLVGL
                     NNIKVDIDNLMKYIRIEKMRKERGMNPNPISLHSVFQGPPGTGKTTVARLLGKIYKSM
                     GLLTSGHLIEVDRSGLVGEYIGHTAIKTNKVIDSALDGILFIDEAYALIPEGGKGNDF
                     GQEAVDTILKRMEDLRDRLVVIVAGYPNEMNRFIQSNPGLQHRFNRYFTFNDYTPDEL
                     LEIFRRIAESAHFIIAPDAMEKVSKYLNYVYQSRTDTFGNARLVRNLFEEVVQLQSAR
                     LAEMEHITDQDLCTITLDDINKAISDEYQEEITESLDEIMAELNGLIGLDNIKKDVEQ
                     LLNYIRVEKLRSEQGMTTQSLSLHAVFQGPPGTGKTTVARLIGRIYKSLGLLPKGHVV
                     EVSRNDLVGEYVGHTGPKTERVIDSAMHGILFIDEAYSLNPKGSQGNDFGREAIETLL
                     KRMEDDRDKFVVILAGYPDEMKDLIETNPGLRSRFNRYFNFVDYKPDELMDIFEGFIK
                     KRAMTLEPAAEQRLIGHFIDVWEARDKHFGNGRFARNLFEKVLQAQSDRVAKMTEISP
                     DDLRLIRLEDVESVISIVKPDQPKERNTIGFKPKG"
     gene            complement(6550..6912)
                     /locus_tag="PLF20_05395"
     CDS             complement(6550..6912)
                     /locus_tag="PLF20_05395"
                     /inference="COORDINATES: protein motif:HMM:NF039385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6232 family protein"
                     /protein_id="HOX80473.1"
                     /translation="MAEEKIYFDEGNVKVTNARFLTYGKMHSMANVTSVAKYVIKPKR
                     TGPIVLGIIGLICFAFKWWLAVLLLVGAIAWWMTQKNKYLVSLASASGSEEALTSADE
                     AFIDRVVSALNEAIAERG"
     gene            7223..9124
                     /locus_tag="PLF20_05400"
     CDS             7223..9124
                     /locus_tag="PLF20_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HOX80474.1"
                     /translation="MSEPIKHECGIAFIRLLKPLEYYLGKYGTSLYGLNKLQLLMEKQ
                     HNRGQDGAGVACIKLGLLPGKKYINRVRSNATTSIKDVFDQIFAPFRQLREDHPHRLN
                     DVQWLKENIDFTGEIFLGHLRYGTYGKNSIQNLHPFLRENNWKTRNLVLAGNFNLTNV
                     DELFKTLVDLGQYPVETSDTVTVLERIGHFVDEENERLFKAFRAKGYSKKEITDFIIT
                     HFDLKSVLTRAASKWDGGYVMAGIIGHGDAFIMRDPAGIRPAYFYADDEVVIATSERP
                     VIQTTLNVKAHQIDEIPPAHFLQVKSNGEWSLQQFAEPIRTASCSFERIYFSRGTDQE
                     IYHERKQLGKMLLPSILKAIDYDLENTVFSYIPNTAAVAFQGLMEALAEFCDEKRKEK
                     LLALDYTPTKNEIDEIFNIKPRFERIAVKDIKIRTFIAEDEQRDDLVAHVYDVTYGAI
                     RAGIDNLVVLDDSIVRGTTLRKSIIRILDRLEPKHIIVASSAPQIRYPDCYGIDMAKL
                     SDFIAFKAAIELLKETNQAHIINQVYRMAKAQENLPREQIQNPVKKIYEPFSDEEISN
                     KISQLVKNHQVKSRVSVIFQTVDHLHQAIPHHSGDWYFTGNYPTPGGNKVVNKSFINF
                     IEGKNERGY"
     gene            complement(9158..9553)
                     /locus_tag="PLF20_05405"
     CDS             complement(9158..9553)
                     /locus_tag="PLF20_05405"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOX80475.1"
                     /translation="MPSIFSKIVNGEIPAYKVAESNDYLAFLDINPLCKGHTLVIPKK
                     EIDYIFDLNDDDLQGLILFAKKVAKGIEKTIPCKRIGIAVIGLEIPHAHIHLIPLQGI
                     YDIDFSKPKLKLPAEEMRTIAEKIAANIG"
     gene            complement(9567..10040)
                     /gene="greA"
                     /locus_tag="PLF20_05410"
     CDS             complement(9567..10040)
                     /gene="greA"
                     /locus_tag="PLF20_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOX80476.1"
                     /translation="MSEIQYYTKQGLESLKQQLDYLTTVERSRISQMIAEARDKGDLS
                     ENAEYDAAKEQQGLLELKISKLQNLLLNARIIDDVDIDVHQVSLFTTVKLKNIKTGAI
                     TSYSIVPESEANLKAGKISVTSPIAKGLLGHKSKEEVAVKVPAGTLHFQILEITR"
     gene            complement(10142..11206)
                     /locus_tag="PLF20_05415"
     CDS             complement(10142..11206)
                     /locus_tag="PLF20_05415"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOX80477.1"
                     /translation="MKQIYKYISAIVLAIIIFNPFEEIQAGNKDRSGQAGASELLINP
                     WARSSGWGGVNTASVRGLEAMFGNVAGVAHTGSTELIFAQTQWLKGTDITISAFGLTQ
                     RISESGVLGLSVMSMSIGDIMITRTDLPEGGIGTFSPSYLNVGISYSQAFSSSIYGGV
                     LIRIINESISDASAGGIAIDAGIQYITGDQENIKFGIALRNVGPTISFSGDGFATRVS
                     IPGQETLFTLTERQNKFELPTTMSIGAAYDFLFGGSRFTLAGNFQSNAFSNDLFMLGG
                     EFSFRDYVMLRAGYAYEDGIFEDIESSNKITVSKGFSGGLTVQVPFNKEKGSNFSVDY
                     SYRSTEHFDGTHTIGVRIAL"
     gene            complement(11260..>11782)
                     /locus_tag="PLF20_05420"
     CDS             complement(11260..>11782)
                     /locus_tag="PLF20_05420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80478.1"
                     /translation="MPIMDGTHSENWLSNDARFRIRVSKPYLRYYSRPLPPGSTDTTN
                     NNFPLYGFSTEGLEPIYNDIVKIQKDLDLINIVPNPYYAYSSYENNALDNRVKFTNLP
                     KKCIITIYNMSGTLIRQFTKDDPTTYLDWDLKNFAGIPVAGGVYIVHVKSDGGERILK
                     WFGALRPIDLNTF"
BASE COUNT         3418 a         2531 c         2303 g         3530 t
ORIGIN      
        1 ccggttcaat tcaggcgcca ctgaaaactt tgatatggat tatgattctt attttctggc
       61 aggctttgcc ccaaatttct attctgtagc aggcgatgaa cattttgcct tgaacaccct
      121 gccggtactt acggaaggat taagcattcc actgggtttt gttaaaaatg cttcatccgg
      181 ttttaatatt gaacttcagg aaaccatgga gggtattact atttatctta ccgatttaaa
      241 gacaaatacc aaccagaatc tcagtaagag ccctgtttat accttcactg ccgaagaagg
      301 cgacctgccc gaccgcttta agctaacctt tggcaacgtg ggattcagcg aaccttcaat
      361 cagtccgttc agcatctatt ccggcaacgg cgccatatac atcaacaacg ggaatcaaat
      421 tgttgaaggg actgtcacca tttattcgat aaccggtcag gcaatcacta cccgttcgct
      481 ttccggcgac cgcttgcaaa agctcagttt caatggcaaa ccgggttgtt atatcgtaag
      541 ggttacaacg gataaaggtg tttattcaca aaaaataata ttataaacca tgaaaaaaat
      601 aattcgcaac atcaccttaa ttcttttcct gcttttcggt tcagcaacca tcaataatgt
      661 tttagccgac gatccggctc ctccgcctct accgggcgag catggcatgg gcggaaacaa
      721 ggctcctgaa ggcggcggcg cacccattgg aagtggattg ggaatactgc ttggccttgg
      781 ccttgcttat ggtgctacca aagtcatacg caagaatagc gagaaagtca taagcaagaa
      841 tagcgaggag gaatagtgta acaggccgct ctttgttgca cttgattgcc ctaaacatga
      901 ggccggtcta aaaagccgta agacacttaa accctcccgc aaaatcggga cgggcactga
      961 aagggattta cccaggtttt gggtttttat actaaactca agttagacta aaaaatctga
     1021 ggctgtcatt aaaaattgag tgacagcctc ttttttcaaa tcccaacctc tttgcaatta
     1081 ctaccttcta tttcttctta aaaaccaatt ttcgatattg cgggtccatc cattcgatga
     1141 atatctctct gagctcccca tatttcattg gattcacttc gttctctaca aattctatgg
     1201 tgcgtcttat cttcaccata ttcccatttt tgctcaattg aattctcaca gccccaaagt
     1261 ctctacggat ttcgagattt cttacaggag ttagcaattc gagagtatcg ggaatttgca
     1321 gcgtatagtc gtaatcttct attaaaaaat aagggatggc gaagggagaa atccttgagg
     1381 atggcattga cgcaacgttc cagctggtaa cacctgttcg ggcaaaaggt aactccattt
     1441 cataaaagcc tttagcaagc tgagtcaaag tttgatcggc ttttaccgaa atctcggtct
     1501 gaagcctgtt gagatttgat tgcttcgact taaatgaaga aatattttct ccgctgcaaa
     1561 acccagttaa aatattagta atttccttat cgttttcgag aatacgataa aaaggatgat
     1621 atcgaccggt aagctcaagt tcaccatttc cttccacgga agcagatttt acttcgaaat
     1681 tgccttttac ttttagggtg ctattttcgg gatagatttc ccattttttt acgctttctg
     1741 ctgccggatc gagcaataag agggtatttc cataaaggtc gggtaacaaa ttgtaaggat
     1801 gttcattaat tgccgaaaga taaacacttg ctgagcctgg aatctctgtt ctaacatagt
     1861 atgcatcaat aacgagtgga ttgaaccatt ttgtatccag aacattagtc ggaacagcag
     1921 ccacaatatc ggcttgatac ccggcttgcc tgagcattga agccaagaga atggtttttt
     1981 cgatttctgt tccggaaaga gattcccaaa cctcagcagg tgtcctgaat ttaaatgcaa
     2041 tgacagagga gggaattttc agataagata gctcgttgat gaccaaattt cttatttgaa
     2101 tgattttttt agaatccgat gcatcgcttg ctttgatctg atcaatacga gccatggcag
     2161 tatgaggaag tttagtatca gtaaaggcag gctgattgct gagaagctcc aaacttcgtt
     2221 gtaaagatag agaagtagaa aaactcaaat aaggggaata ataagaatct tcaggcatgt
     2281 agatttcatg aggatttgct gcaactttgc caaaattcca tgtataaatc atcattcctt
     2341 ttgcaaagga tacttctgga ctaagcctca aattcaacat tttataattc aatatcgtac
     2401 cttcaggaac cctcacgatt accttgtacg attcaaccgg aactggaaag gaaagaatct
     2461 cctcgcccat caagtaaggc attcctgtgg gttgactcac gagattataa tcgaattcga
     2521 tagtagcacc cacctcgaga cctgtatgcg aaactaccat ttccctcaaa tgattgtaag
     2581 ccgcactacc gctggcaaac ctcggtaata cctcattaag ggcattgaga ggagaatcta
     2641 ctttcttacc atcagccatt atagtacgcg acttattgac tttcagcctc tgaaattgag
     2701 gattatatac gatgaacgat tccccgaaat acctctgaaa tgcctgatgc gtcaatagtt
     2761 tcatcgaaga agaatagtga taatctacgc taccctcttt cgaaagcgta tattctttga
     2821 taatgtaatt gtaaatggca tcataggcta attcctgtgc cgtacaaaat tttccggcta
     2881 cgactaccag aaataccaac atccatctaa ttctaatttt tatcatattt atcttcttta
     2941 gttcttttcg tctgttatgc gattcctcac tagcaatttt acaagcctgt tcagaaattt
     3001 tctataacca tattcccgga aagaatgatt gacaaagaaa ggacatttta tcgaaacagt
     3061 gtgtaaaaac taaagcaaaa tctatatcag gcttagtttg agcacctaaa tctcaagtca
     3121 actacatcct gcagtgattt gctgaccata ttcaaatagg acaagttcgg attgaaaaga
     3181 atatctatcg atttcaagat aatcattttc agcgcatctc gaataaaatg atgttttgag
     3241 gctctattga aaaaagcaag acgtaaaatg attaattctt agcctttggg tttgaatcca
     3301 atagtgtttc tttccttagg ttgatccggt tttacgattg agattacact ttccacatct
     3361 tctaaacgta taagccgcaa gtcatcaggt gaaatttccg tcatttttgc tactctatcg
     3421 ctttgggctt gtaaaacttt ttcgaagaga tttcgagcaa agcgtccatt gccaaaatgt
     3481 ttatctcgtg cttcccaaac gtcgataaaa tgccctatca ggcgctgttc agcagcaggc
     3541 tccaaggtca tggcacgttt ttttatgaaa ccttcgaaaa tatccatcaa ctcatcgggt
     3601 ttataatcca cgaagttgaa atagcgattg aagcgagatc tcaggccggg attcgtttcg
     3661 atgaggtctt tcatttcgtc ggggtagcct gccagaatga cgacaaattt atcgcggtcg
     3721 tcttccattc gctttaggag agtctctatg gcttctcggc caaaatcatt gccctgactg
     3781 cccttgggat tgagtgaata ggcttcgtca ataaagagga ttccgtgcat ggctgagtca
     3841 atcactcttt cagtttttgg accagtgtgt cctacatatt cgccaaccag gtcgttgcgt
     3901 gatacctcca ccacatgtcc tttaggcaat aaacccagag atttataaat acggccaatt
     3961 aaacgcgcaa ccgtagtttt cccagttccc ggaggtccct ggaatactgc atgaagactc
     4021 agactttgtg tggtcatccc ctgttcggaa cgcagttttt caactctgat atagttcaaa
     4081 agttgttcaa cgtctttttt gatattatcg aggccgatca gtccattcag ttcagccatt
     4141 atctcgtcga ggctctctgt aatttcctcc tgatactcgt cggaaatggc cttgttgata
     4201 tcatcaagtg taatagtaca taaatcctga tcggtgatat gttccatttc ggccagacga
     4261 gccgattgca attgtaccac ctcttcgaaa agatttctaa ccaaacgggc attcccgaaa
     4321 gtgtcggttc tcgattgata tacatagttc aaatatttcg aaactttttc catggcatcg
     4381 ggagctatga taaaatgagc tgattcggca atccgtctga agatttcgag caattcgtca
     4441 ggagtataat cattgaaagt aaaatatcgg ttaaaacggt gttgaagtcc gggattcgac
     4501 tgaataaaac ggttcatctc gtttgggtat ccagcaacga taactaccaa tctatctcgt
     4561 agatcttcca tacgtttcaa aattgtgtcg acggcttcct ggccgaagtc attccctttt
     4621 cctccttcgg gaatcaaggc ataagcctcg tcgataaaaa gaattccatc gagggcgcta
     4681 tctattacct tattggtttt gatggcggta tggccgatat attctcctac gaggcccgag
     4741 cggtcaacct cgatgagatg tccactagta agcagtccca tggatttgta tatttttccc
     4801 agcaaacgag ccacggtagt ttttcctgtt cccggaggac cttgaaagac cgagtgcaaa
     4861 gagattgggt tgggattcat tcctctttcc ttacgcattt tttcgatcct gatgtatttc
     4921 atcaaattgt caatgtccac ctttatattg ttgagcccta ccagtttgtt gacttcggcc
     4981 agcaattcat cgagcgattt attggtttcg ggctgagatg caggtgatga agatggcttg
     5041 ggtttatcct cgggattggc ttctgcctcg gctatggcgt ctttgaaaag ggattcatcg
     5101 ggctcgagct tcgatgccag cttgaaatat tccaatgccc tcttggcctt gcctttttcg
     5161 agaagcgctt ttccggcatg atacaacggc atggcgtctt ccacttcctt caaggtagcc
     5221 gatgggcatt ccaaggcagt ctgataaatt tcttccgtct tccccatatc attcaacgta
     5281 tagactaaat tgctgaaaac aaccggattg gagtgatcga gtcgatacga atcttcataa
     5341 tatttcaagg ctttatccat atctccaagc cacgaccagg cagctcctat gccatttaga
     5401 gccgaaggaa attctggagt aatcttcagg gcgtcttcaa agtacgaaat agccttttga
     5461 tattcctttc gattgaaata ctccatgccc gtttggaaat atcgaatggc cttcggattg
     5521 acttctatct ccttttgggg agcagccctc tgagatggtt cagcttcatc gggatcataa
     5581 gtaaattctt cctcttctcc agggattcga gtttctatag gtaccgacat aactaccagc
     5641 caaatcccat tcccataacc gatatctgta atttcataac cctcggcaat tttcgatttg
     5701 acttcggcct cggggaattt tttatccgat acataaaatt gattctcgaa aggagcatcc
     5761 ttagtcatta ccaaacccca tttccctttg catggactta tcgaagtaat ataatatccc
     5821 ttcgaccagt actcctttat ggtttttgca ggaaaatcac tgtaaaggcc gaaaatttgc
     5881 tgtccataat gttcggtctt cccaaacact gcggcccatc tatcgtttcc gtaaaccagc
     5941 tgtaaaactg actctcccgc cttaatagct tctttaatct cgccagacgg gaaatcccca
     6001 ttggttatcc atctttggtc ttcaataccg gtgttacctg acatcaccac aaaccaaact
     6061 ccttctccat agccaatcca gctaatatcg agaccatcat tccatccagt agaaataaca
     6121 tccttcggaa attcggttct tgtgctccat cgctgccccg agtaaggact cgaaggatcg
     6181 gtaatcaaca cccatttccc ttctccataa gcaaggctat atatcaattc ttccttatcc
     6241 catgccttgg atatgggatc ctcgggaaag gctttgaaaa attgaaaatt ttttccggcc
     6301 atagtttaac tattttttat tgaatttata atctcttgat aaacaaacta ataccgcaag
     6361 aaaccctatt cgcataattc cccttcgttc tcataattct tgaagaacaa ataaagacat
     6421 aactctctat cttccttttt taccaaagga actaaataca acacgaaacg actaatgatt
     6481 ttcgatcgga aatttacaat taaaggaacc tgccagtaaa tcaattaccg gcaggtttgt
     6541 aaaacatctt caacctcttt ctgcaatggc ttcattcagc gcactgacaa ccctgtcgat
     6601 gaaggcctca tcagcactgg ttaaagcctc ttcgcttcct gaggcactcg ccaaagaaac
     6661 caaatattta ttcttttgcg tcatccacca tgcaattgct ccaaccaaca acaatacggc
     6721 aagccaccat ttgaacgcaa aacagattag gccaattatt cctaaaacaa taggacccgt
     6781 acgcttaggc ttgataacgt atttggccac cgaagtcaca ttagccattg agtgcatttt
     6841 gccataagtc aggaaacgcg cattcgtaac ttttacattt ccctcatcaa agtaaatttt
     6901 ttcttctgcc ataaaaattt tatttttgaa tgtgaataat ggtgtttttg atactgcctc
     6961 aaaaaatttg agatttatcg gatacaaagg taaagttttt ctgataatac aagaatttat
     7021 tattaaaatt tcacaaaaat cttatgaagc agttatagca ggccaaattt cttatgaaaa
     7081 attttcatga aatctcattt ttcttcggaa ggaacattcc ttcgatgatt gtgaccattt
     7141 gatattcttc tatcattggg aattattgag gggttaaaat acgtaatttt gcagcttgat
     7201 tttgtgtaat gtatcctcta tcatgagcga accgatcaag catgaatgcg gcattgcctt
     7261 tattcgactc cttaaaccct tagaatatta tcttgggaaa tatggtactt ctctctatgg
     7321 tttaaataaa cttcaattgt taatggaaaa gcagcacaac cgcggacagg atggagcggg
     7381 tgtggcttgt ataaaactcg gtttactacc tggtaaaaaa tatatcaaca gagttcgttc
     7441 caatgcaact acatccataa aggatgtctt cgaccagatt tttgcgccct tccgacagct
     7501 acgcgaagat catccccatc gtctgaatga tgtgcagtgg ctgaaggaaa atattgattt
     7561 taccggagaa attttcctgg gacatttgcg ctatggtaca tacggaaaaa attccataca
     7621 gaatcttcat ccgtttttac gggaaaataa ctggaaaacc agaaatcttg ttttagcggg
     7681 caatttcaac ctcaccaacg tagatgaact tttcaagact ttagtcgatt tggggcaata
     7741 tcctgtcgag acttctgaca cggttactgt tctcgaaaga attggacatt ttgtggatga
     7801 agaaaatgaa aggttattca aagcatttag agctaaaggc tattcaaaga aagagattac
     7861 cgactttatc atcactcatt tcgatttgaa gagtgtttta accagagcag cttcgaaatg
     7921 ggatggaggc tatgtaatgg ccggcataat aggtcatgga gatgccttta ttatgcgtga
     7981 cccggcagga atacggccgg cctactttta tgccgatgat gaagtagtaa tagcaacttc
     8041 cgaaaggcct gtcatacaaa ccactctgaa tgtcaaagcc catcagatag atgaaatacc
     8101 accagctcat tttttgcagg taaaaagcaa tggagaatgg tcattacagc aatttgctga
     8161 accgatacga actgcctcat gctcattcga acgaatttat ttttcgagag gaaccgacca
     8221 agaaatatat cacgaaagga aacaattggg taaaatgctc ctgccttcta ttctgaaggc
     8281 aatagattat gatctggaaa atacagtttt ttcatatata cccaatactg ccgctgttgc
     8341 atttcagggt ttaatggaag cccttgccga attttgtgat gaaaaacgga aggaaaaatt
     8401 actggcactc gattatactc ccactaagaa tgaaatagat gagattttca atatcaagcc
     8461 tcgattcgaa agaattgcag taaaggatat caaaatcagg acctttattg ccgaagacga
     8521 acaaagggat gatctggtag ctcatgttta cgatgtgact tatggagcta tacgtgcagg
     8581 aatcgacaat ctggtagttc tggacgattc cattgtgagg ggtactacac ttagaaaaag
     8641 catcatcaga atactcgatc gcttagagcc caaacatatc atcgttgctt cgtctgctcc
     8701 tcaaatccgt tatcccgact gttatggaat agatatggcc aaactttccg attttatagc
     8761 ttttaaggct gccatcgaac tgctcaaaga aacaaatcaa gcacatatca tcaatcaggt
     8821 ttatcgcatg gcgaaagccc aagaaaatct cccccgggaa caaattcaaa acccggtaaa
     8881 aaaaatttat gaacccttca gcgatgaaga aatttcgaac aaaatcagtc aattggtcaa
     8941 gaaccatcag gtcaagtcaa gagtgagtgt aatatttcaa accgttgatc atcttcatca
     9001 ggctatacca caccattcgg gcgactggta ttttacgggt aattatccta cgcctggtgg
     9061 caacaaagta gtcaataaat cattcatcaa ttttatagaa ggaaaaaatg aaagaggcta
     9121 ttgaaccctc ttcctctcta tactcaaaat aaagtattta gccaatattc gctgctattt
     9181 tttcggctat ggttctcatt tcttcggccg gtaactttaa tttaggcttc gaaaaatcga
     9241 tatcatatat accctgcaga ggaataaggt gaatatgggc atgtggaatt tcgaggccga
     9301 ttacggcgat tccaattctt ttgcaaggaa tggttttttc gattccttta gccaccttct
     9361 tggcaaagag gatcagtcct tgtaagtcat catcattcaa gtcgaaaata taatctattt
     9421 cttttttagg aatgaccagg gtatgacctt tacaaagagg attgatatcg agaaaggcca
     9481 gataatcatt ggattcggct actttatagg caggaatttc accattcaca attttcgaaa
     9541 atatgcttgg catttttagg ttgggattaa cgggttattt caagaatttg aaaatgcaaa
     9601 gttcctgccg ggacttttac tgcgacttct tccttggatt tatggccaag caagccttta
     9661 gcaatgggtg atgtgaccga aatttttccc gcttttaaat tggcttcgct ttcgggtaca
     9721 atagaatagg atgtaatagc acccgttttg atatttttca actttacggt agtaaagagg
     9781 gaaacctgat gtacatctat gtcaacatca tctattatac gagcattcag taagagattc
     9841 tggagtttcg atatctttaa ttccaataat ccctgttgtt ctttagctgc gtcgtattca
     9901 gcattttctg ataaatcgcc tttatcgcgg gcttcggcta tcatctgaga aattctcgac
     9961 ctttccacag tagttaggta atcgagctgt tgtttcaatg attccagccc ttgttttgtg
    10021 taatactgaa tttcgctcat gttgtaaaca atttaccttg gttttttgta ctaaagaaaa
    10081 gaagaccctt tttgcatgaa agggtcctct tttttgatgt tgcaaaatta ctaaaaagtc
    10141 cttacaaagc tatccgaact ccaatagtat gggtgccatc gaaatgctcg gtcgatctgt
    10201 aagaataatc aactgagaag ttcgagcctt tttccttgtt gaaaggaact tgaactgtca
    10261 atccgccact gaatcctttc gaaacagtga ttttattact gctttctata tcttcgaaga
    10321 taccgtcttc gtaggcatat ccagctctga gcataacata atctctaaaa gaaaattctc
    10381 ctcccagcat gaacaggtca ttactgaaag cgttggattg aaaatttccg gcaagggtaa
    10441 atctcgaacc accgaaaaga aaatcgtaag ctgctccaat gctcatggtt gtgggaagct
    10501 cgaatttgtt ctgacgttcc gtcaaggtaa ataaagtttc ttggcctgga atactcacgc
    10561 gagttgcaaa accatctccc gaaaatgaga tagttggccc cacatttctc agagcaattc
    10621 caaacttgat gttttcctga tctcctgtaa tgtattggat acctgcatct atggcaattc
    10681 caccagccga agcatctgaa atagattcat tgataatgcg gatcagaaca cctccataaa
    10741 tgctggagga aaaagcctga gaataagaaa ttcctacatt gagataactg ggagaaaacg
    10801 tacctattcc accttcggga aggtcggttc gggtaatcat gatatcgcct atggacatcg
    10861 acattacact cagtcccaaa acacccgatt cactgattct ttgagtcaaa ccaaaagctg
    10921 aaatggtaat atcggttcct ttcaaccatt gagtttgtgc gaatatcaat tcagtgctgc
    10981 ccgtgtgagc aacacctgct acattgccaa acatggcctc gaggcctctg accgaggccg
    11041 tattcacacc accccagccg ctgctgcgag cccagggatt gataagaagt tctgatgctc
    11101 cggcttgtcc ggaacgatcc ttgttgccgg cctgaatttc ctcaaagggg ttaaagatga
    11161 taatggccag aactattgcg gaaatatatt tataaatctg tttcatacct tttgtttttt
    11221 gagtgattaa acgtttagca tttatcatat cgttgaattt tagaaggtat taagatcaat
    11281 aggccgcaaa gcaccgaacc atttcagaat tcgctcacct ccatcgcttt tgacgtgaac
    11341 gatgtaaacc cctcctgcaa cgggaattcc ggcaaaattc ttcaaatccc aatcgaggta
    11401 agtggttgga tcgtctttag taaactgacg tataagagtt ccactcatat tgtaaatcgt
    11461 aataatgcat tttttgggca ggttggtaaa tttcaccctg ttatccagtg cattgttttc
    11521 gtaagaagaa taggcataat aaggattggg gacaatattg atcaaatcga ggtccttctg
    11581 gatttttaca atatcattgt aaataggctc gagaccttcg gtcgagaatc catacaatgg
    11641 gaagttatta ttggtcgtat cggtacttcc cgggggtaaa ggtctggaat aataacgcaa
    11701 atacggctta gaaaccctta ttctgaaacg ggcatcgttc gaaagccaat tttcggaatg
    11761 cgtgccatcc atgataggca tt
//
LOCUS       DAOORU010000063        11716 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_6826_length_11716_cov_5.347312, whole genome shotgun sequence.
ACCESSION   DAOORU010000063 DAOORU010000000
VERSION     DAOORU010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11716
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6826_length_11716_cov_5.347312"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /gene="sprA"
                     /locus_tag="PLF20_05425"
     CDS             complement(<1..1619)
                     /gene="sprA"
                     /locus_tag="PLF20_05425"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOX80479.1"
                     /translation="MKTSIIPFIRYFVALGFVVALSGFSAADSKASSTDYSLAFFAPP
                     DTIPLRYPFKDRDLYSPPASEHQLFLRNPSSIKVNVEYDGESNQYILTETMGSLQVRP
                     PLVMDFKEYMEYDASRALNSYWREKSLSTKGEKSDGIIPSIYIGGQAFDKIFGGSTID
                     VRPNGSAELIFGIRSNKNEDPSLAVRQRKTVNFDFQEKIQMNVIAKVGDKIEFKANYN
                     TESSFDFENKIQLKYEGKEDEILKLLEVGNVNLPLNSTLIQGSQSLFGVKTKMQFGRT
                     TITAVVSQQQSETKNITVEGGAQTNTFKLTADQYEENKHFFLSQYFYDHYNQSLSTLP
                     IVQSNVNITKIEVWVTNIGAAVTDNRNIVAFQDLGEKNPYHPALSTTMAAPGYPSNNA
                     NNLLYYLDVNQLRNINTVSNYLKSNDFTAGQDFEKIESARKLQSTEYTFNSLLGFISL
                     NTTLNPDQSLAVAYQYTIVGDTTVYQVGEFSDGGISSPNCLVAKLLKSTAVNTRIPLW
                     KLMMKNVYAIGAYQVNRQDFILNLLYSGNNNG"
     gene            complement(1640..2224)
                     /gene="ruvA"
                     /locus_tag="PLF20_05430"
     CDS             complement(1640..2224)
                     /gene="ruvA"
                     /locus_tag="PLF20_05430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOX80480.1"
                     /translation="MYAYLEGQIAELNPAYAVIDCNGVGYEVNISLNTFSSIKDKERV
                     RLYTHLVVRDDAQMLYGFAEESERTLFRHLITVTGIGPSTARMILSSMTPVELISAIA
                     SGNEEILKRVKGIGTKTAQRILIELKDKVGKVTTSQGSLPVLGNRNLEEALSGLVILG
                     FSKTLVQRALEKIVSTRGDSISVEEMIKEALKVL"
     gene            2410..3720
                     /locus_tag="PLF20_05435"
     CDS             2410..3720
                     /locus_tag="PLF20_05435"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80481.1"
                     /translation="MKKFCFFLIVSLYAPIASSQPALVNEWWDQAKGIKKQEGQILDT
                     LRIGHWITWYPDGTKESEGNYHDGNKAGKWEYWFPDGKLNSILNIENGPCILYHPNGK
                     ICMEWQVIHGKRNGKLEEYDTAGHLIRQITYQNGLKQGFVQTFYPTGQKSFEGIYRND
                     SLNGKAIWYYPQGNKEMEGNLHNDLQEGWWIFYHPNGTKGSEGKFKEGKRTGKWVFYY
                     ETGEKWREGEYADDQRQGHWITFYETGQRFREGNFVNDKEQGLWVEYYQNENINYQGY
                     FEGGLMEGLWEAWYEDGKTRYRNHYSLGIPHGKWTYFYRNGNKKYEGYYVNGRKNGLW
                     TEYYQDGSMESKANYTDGRRQGTAFFYEPNGLLIRQQDFLNDEPHGSFISFYPNGKKS
                     SEGVHYRGSPTGIWKFYSHDGKLIMTKTYSNPPSSSKHQKEKKN"
     gene            complement(3775..4830)
                     /locus_tag="PLF20_05440"
     CDS             complement(3775..4830)
                     /locus_tag="PLF20_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX80482.1"
                     /translation="MKKIFRFSDSRKVASLTAFSTIVAYGIFLVIINLIYQQPVGIQF
                     LLLGLGIWVAVYFITLFFVDKFIFSKIRVIYKTIHSQKVPKGIDPRQTIEAHSVEDVQ
                     REVTEWSDERREEIETLKELEKYRREYIGNISHELKTPIFNIQGYILTLLDGGLEDPN
                     VNRNYLLKAEKSVNRMIAIIEDLEMINLLEANELKLNIIRFNLVDLAREAVESLEMKT
                     KKRECEVFINHNIDRPIYVLADRQRILQVFTNLIDNAIKYGKKSGGRIKISFFDMDEN
                     ILAEITDDGPGISQNDLTHIFERFYRLDRGRSREMGGTGLGLAIVKHIIEAHHQTVNV
                     RSSIGVGTTFGFTLKKA"
     gene            complement(4876..5568)
                     /locus_tag="PLF20_05445"
     CDS             complement(4876..5568)
                     /locus_tag="PLF20_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOX80483.1"
                     /translation="MEASAYKILVVDDEPDVLEFLGYNLQREGFLVFTANNGFEAIEK
                     VKESKPHLVLLDLMMPGMDGMETCQELRKIVSGQDMLIVFLTARGEDYSQIAGFEMGA
                     DDYVTKPIKPKVLISRIKALLRRIQPAELEKIIRFEGLTIDRERYVVIQNDEEIILPR
                     KEFELLSLLASKPDRVFTREEIFEQVWGNDVIVGERTIDVHVRKIREKLGISIITTVK
                     GIGYKFENIGDE"
     gene            5786..6388
                     /locus_tag="PLF20_05450"
     CDS             5786..6388
                     /locus_tag="PLF20_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80484.1"
                     /translation="MSNWLEEAERRSRREEFKGQSERLQVRIDRVRENYQKNQEVYDA
                     FIGKIKSLINRANALPAEFREPFGKIKYKTKQTRLNNYLNIFSSSRHIRSNKSKRLWA
                     KLFPTHFKHIRVVFINVSKHLDKIDLEIRQDLFERKRINEDGNTGKHHHKNKKDHIDL
                     VIHLDIILLNDELAQKILDFLAFRCELNDFPIPFEEAIYY"
     gene            complement(6418..7329)
                     /locus_tag="PLF20_05455"
     CDS             complement(6418..7329)
                     /locus_tag="PLF20_05455"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOX80485.1"
                     /translation="MIETNRIGTLKWHHILNPSEQDFDYLLEHFHFHPLDIEDCRSLV
                     NQRPKIDIYDDYYFLILHFPYFDKTRNFVRTREEKIFWGKDYVITMGKIHSEVSEFFL
                     KTRQKIEQHIDLDFATSDALLYRILEYLMKESYTLVRYVGDQVDQAGRMLFERKTLQT
                     IESISVTRRNIIILNTIFKPQLPVFNKFESGEIQSFAESMEEYWGNILDYYQKIWDLI
                     EDYSELIEGYSKTFDSLQTNRTNEIVKTLTLVSTIVLPLTFITSVYGMNILLPLQENK
                     YAFHIITAVMVALAGFMLFYFKKRHWM"
     gene            complement(7477..8736)
                     /locus_tag="PLF20_05460"
     CDS             complement(7477..8736)
                     /locus_tag="PLF20_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80486.1"
                     /translation="MDEEPEISGKNTETDQNILDEPEIKVESGLEIVEPSDSSISSKP
                     QVQVLLSSQWVKSSERKHSHHKKHKGGHRKSRYKKPKKSSGFKLFFKNFFIHIKEPLY
                     NFLFFLNFRSAPYDPFLDSETVQPEERKPMRIQRYAAYVIQSTLVFIISYIIAYFIYQ
                     FAIIFMAAHFGIDSVLYYYEVMFPIGDASNLWTPFNIIMITLAGPVVSLIFGLIWYRW
                     VMRKVKRPAMKLFFLWLAFHSFNMFFGAFVAGVITNQGFGYVANWLYLGIPLKIFFSL
                     IALGILTYLGWHSTPHILSTTNSPRRINRYNRSYFILSQMIIPWFIGSLLMLWIKIPN
                     RTPQHENILLYDIIILVAMAFLVIPPLFNHQNQPSESKKRYLHKKTSFAFLYLFIAAI
                     LIIAYRFGLERGLHIVMRFDLSLSFYH"
     gene            9771..10655
                     /locus_tag="PLF20_05465"
     CDS             9771..10655
                     /locus_tag="PLF20_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX80487.1"
                     /translation="MMPWGELTAMLTAIFWTATAMAFEVASKRVGSLRVNTLRLPMAF
                     IYFFIFCWIYRGHPLPIDAPLHAWIWLILSGLIGFVFGDYFLFKSFTIMGSRTTMLVQ
                     TLVPPITALVGFILLSEKLSLKHIAGMTLTLSGIFLVVLTTNNGKLGLKIPRRGLLYA
                     IFAALGQSIGLVLSKFGMRDYDAFASSQIRVMAGALGFPILSFALHQSNSLKKTFHDK
                     VAMKSILIGSIFGPFLGVSFSLIAIQHTKTGIASTLMALTPVMILLPSAILFHQKIVW
                     KEIVGAIISVSGASLFFL"
     regulatory      11222..11322
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            11522..>11716
                     /locus_tag="PLF20_05470"
     CDS             11522..>11716
                     /locus_tag="PLF20_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009000891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOX80488.1"
                     /translation="MGKTIYEIIQERVLVMDGAMGTMIQRYQLKEEDFRGELLKHHPF
                     DLKGDNDILCLTQPDLIKEIY"
BASE COUNT         3668 a         2374 c         2211 g         3463 t
ORIGIN      
        1 ccattgttgt tccccgaata cagcaaattc aaaataaaat cctgcctgtt tacctgataa
       61 gccccaatag cataaacatt tttcatcatg agtttccaaa ggggaatgcg agtattcaca
      121 gcagtacttt tcaaaagttt ggccaccaaa caattgggag agctgattcc accgtcggaa
      181 aattcaccta cctggtaaac cgtagtatcg cctactatgg tatattgata agcaactgcc
      241 aaactttgat cgggatttag cgttgtattg agcgaaataa atcccaacaa actattgaaa
      301 gtatattctg tagactgaag ttttcgtgca ctttctattt tctcgaagtc ctggcctgca
      361 gtaaaatcat tcgatttcaa ataattggaa accgtgttga tatttcttaa ttggttgaca
      421 tccaggtagt aaagtaagtt gttggcattg ttcgaaggat acccaggagc tgccatggtg
      481 gtactaagtg cgggatgata agggtttttt tcaccaaggt cttgaaaagc cacaatgttg
      541 cggttatcgg ttactgcagc tccaatattg gtaacccaca cttcgatttt ggtgatattg
      601 acattcgatt gaacgatagg aagagtagat aaagattgat tataatgatc gtaaaaatac
      661 tgcgacaaga agaaatgttt attttcttca tattgatcgg cggtgagttt gaaggtgtta
      721 gtctgagcac caccttcaac ggtaatattt ttcgtttcgc tttgctgctg cgaaaccacc
      781 gcagtaatgg tagtacgtcc aaattgcatc ttggttttta caccgaacaa gctctgactg
      841 ccctgaataa gcgtagaatt caaaggaagg tttacgttgc caacttctaa aagtttcaaa
      901 atttcatcct ctttcccttc gtatttgagc tggattttgt tctcgaaatc aaaactcgat
      961 tcggtattgt aattggcttt gaactctatc ttatcgccca ctttggcaat gacattcatt
     1021 tggatttttt cctgaaagtc gaagtttacg gtttttcttt gtctgactgc aaggctagga
     1081 tcttcatttt tattggatcg aatgccaaaa atcagttcgg ccgaaccatt gggacgaaca
     1141 tctatggtgc ttccgccaaa gatcttatcg aaagcctgtc ctccaatata aatggaaggt
     1201 ataataccat cgctcttttc tcccttagta ctcaaagatt tctcacgcca gtaactgttc
     1261 agagctcgcg atgcatcata ttccatatat tctttgaaat ccatcaccaa tggaggcctt
     1321 acctgaagcg aacccatagt ttcagttagg atatattgat tcgattctcc gtcatattct
     1381 acattcacct ttatgctcga ggggtttcgc agaaacaatt ggtgttcgga ggctgggggc
     1441 gaataaaggt ccctgtcctt gaaaggatag cgtaaaggaa tagtatcggg aggcgcaaaa
     1501 aatgcaagtg aataatccgt agaactggct ttcgaatcgg ctgcggaaaa acccgaaaga
     1561 gccaccacaa agccaagagc aacaaaataa cggataaatg gtataataga cgttttcaag
     1621 ccaactgata gttttgtttt tacaatacct ttagtgcttc cttgatcatc tcctccaccg
     1681 agatagaatc tccccgtgtg gaaacgatct tttccaaagc tcgttgaact aaggttttac
     1741 taaagcccaa aatgaccaaa ccagataacg cttcttctaa atttctattg cctaaaacag
     1801 gtaaacttcc ctgagaagtt gttaccttcc ctactttatc ctttaattcg atgagaatac
     1861 gctgagcagt tttagttcca atgcccttta ctcgtttcag aatctcttcg tttccgcttg
     1921 caatggccga aatcagttca acaggagtca tcgaggatag aatcattcgg gcagtactag
     1981 ggcctatgcc cgtcaccgtg atcaaatgcc tgaataaagt acgttcgctt tcctccgcaa
     2041 aaccataaag catctgtgca tcatctcgca caaccagatg agtataaagc cgaacccttt
     2101 ctttatcctt tatactcgaa aacgtattca acgaaatgtt tacttcgtaa cccactccat
     2161 tacagtcaat tactgcataa gcaggattca attcggctat ctgtccctca aggtaggcgt
     2221 acataaattt agcagaaatg gctggtttag ttaataaaag gaagaattcg aacttccttg
     2281 gctagcaaaa ttacaatttt ccgatttggg aataaaatca tcatccctgc caaaatttga
     2341 tcttatccta aatcaattac aatttcgagc taattttgca gcttgtggtt ttttgaattt
     2401 accaaaccaa tgaaaaaatt ctgcttcttt cttatagttt ctttatatgc tcctatagct
     2461 tcatctcaac cggcgctggt aaatgaatgg tgggaccagg caaaaggcat taaaaaacag
     2521 gaaggacaaa tcctcgacac tttaagaata ggacattgga tcacatggta tcccgatgga
     2581 accaaagaat cggaaggcaa ttatcatgat gggaacaaag ctggcaaatg ggaatattgg
     2641 tttcccgatg gaaaattaaa ctcaatactg aacatcgaaa atggtccttg catcttatat
     2701 catccaaatg gtaaaatttg catggagtgg caagtcattc atggcaagcg taatggaaag
     2761 ctcgaagaat atgatactgc tggacatctg attcgtcaaa tcacttacca gaacggacta
     2821 aaacaagggt ttgtgcagac gttttacccg acagggcaaa aatcattcga aggcatatac
     2881 agaaacgata gcctcaacgg gaaagccatt tggtattatc cgcagggaaa taaagaaatg
     2941 gaagggaatt tacataacga ccttcaggaa ggatggtgga ttttttatca tcctaacgga
     3001 acgaaaggta gtgaaggaaa atttaaagaa gggaaaagaa cagggaaatg ggtgttttat
     3061 tacgaaacag gagagaaatg gcgcgaaggt gaatatgcag acgatcaacg tcagggtcat
     3121 tggataacat tttatgaaac tggccaaagg tttcgagaag gcaacttcgt caatgacaag
     3181 gaacaggggt tgtgggtcga gtattatcaa aatgagaata tcaactatca gggttatttc
     3241 gagggaggat taatggaagg tttatgggaa gcatggtatg aggatgggaa aactcgctat
     3301 cgaaatcact attcacttgg tatcccccat ggtaaatgga cttatttcta tcgcaacgga
     3361 aataagaaat acgaaggata ttacgtgaat ggcaggaaaa atggtctttg gacagaatac
     3421 tatcaagatg gatcgatgga aagcaaagcc aattatacag atgggcgtag acaaggaact
     3481 gccttctttt atgagcccaa tggcttattg attcgtcagc aagattttct aaatgacgaa
     3541 cctcatggta gtttcatatc tttttatccc aacggtaaga aaagcagcga aggcgttcac
     3601 tatcgaggca gccccactgg tatctggaaa ttttacagcc atgatgggaa attgataatg
     3661 actaaaacat acagtaaccc accctcttca tcgaaacatc aaaaagagaa gaaaaattag
     3721 ttgattctcg aataaggatg aaatgtaatt gccggtaaga attcttagca aggttcaggc
     3781 ttttttcaat gtaaagccga aggtagttcc cacgcctatg ctgctacgca cattcacggt
     3841 ttgatgatgg gcttcgatga tatgctttac aatggccagt ccaagtcccg tgccgcccat
     3901 ttctctcgaa cgacctctgt caaggcggta aaatctctcg aatatatgag ttagatcatt
     3961 ctgtgatata cccggcccat catcggtaat ctcagccaaa atgttctcgt ccatatcgaa
     4021 aaaacttatc ttgattcgcc ctcctgattt tttaccatat tttatagcat tatcgatcaa
     4081 attggtaaaa acctgaagta ttctttgcct gtcggccaaa acataaatgg ggcggtctat
     4141 gttatggttg ataaacactt cacattctct tttcttagtc ttcatctcga gagattcaac
     4201 ggcttccctt gctaaatcca cgagattgaa ccgtattata ttgagtttca attcatttgc
     4261 ttccaataaa ttgatcattt cgagatcttc aatgatggca atcatccgat ttacactctt
     4321 ttcggctttc aaaagatagt tccggttcac gtttggatct tcgagccctc catcgagcaa
     4381 cgtaagaata tatccctgaa tgttaaaaat cggagttttc aattcatgag aaatgttacc
     4441 tatatattct ctccggtatt tttcgagctc cttcaaagtc tctatttctt ccctacgttc
     4501 atcgctccat tcggttactt cacgctgcac atcttcaaca gaatgggctt cgatggtttg
     4561 cctggggtct attcccttgg gaaccttttg actatggatg gtcttataaa tgactctgat
     4621 cttactgaaa atgaacttat ccacaaagaa aagcgtgata aaatatacgg ctacccagat
     4681 tccaaggcct aatagaagaa attgaatacc aacgggttgt tgatatatta aattgataat
     4741 gactaaaaaa atgccataag ccacaattgt tgaaaaagca gtaagggaag caactttacg
     4801 ggaatcggaa aaccggaaaa tcttcttcat atatcaggaa caaaactcat aattttatcg
     4861 aaagaacaat catcctcact catcacctat attttcgaat ttataaccaa tcccttttac
     4921 ggtcgtaata atcgaaattc ccaatttttc cctgattttc cgcacatgaa catcgattgt
     4981 tctttcccct acaataacat cattgcccca tacctgttca aaaatctctt cacgggtaaa
     5041 gactctgtct ggtttcgaag ccagcaggct aagcaattcg aattctttcc ttggcaagat
     5101 gatttcttcg tcattctgta ttactacata tcgttctcta tcaatggtca aaccttcgaa
     5161 ccgtattatc ttttccaatt cagctggctg aatacgacgt agcaaggcct taatccgact
     5221 gatcaatact ttgggtttaa tgggtttcgt aacataatcg tcggcaccca tctcaaaccc
     5281 ggctatctga gagtaatctt cgcctctggc ggtgagaaaa acaatcaaca tatcttgccc
     5341 agaaacaatt tttcgaagtt cttgacaggt ttccatccca tccatcccag gcatcatcaa
     5401 atcgagcaaa accaaatgag gcttgctttc cttcaccttt tcaattgctt cgaacccatt
     5461 attggctgta aaaacgagaa aaccctctcg ctgtaaattg tatcctaaaa actcaaggac
     5521 atcgggctcg tcgtcaacca ctaaaatctt atatgccgaa gcttccattt gtaatttatg
     5581 tgtttctact ttttcatgcg aattgctctg ggcaaatgta atctatttat cttgaaacaa
     5641 gattaacaaa gactttatat ataaaaacaa atgtataaag gaatgaaatt tttgttacaa
     5701 tctttttggg tttaaataac caaggccttt aaaataatta aatttgcaag gaattaatgc
     5761 gatagttgac caaaagcaaa agtgcatgtc gaactggttg gaagaagcgg aaagacgttc
     5821 acgaagagaa gaattcaagg ggcaatccga gcgcctgcaa gtaagaattg acagggtaag
     5881 agaaaattat cagaagaatc aagaggttta tgatgctttc atcggtaaga taaaaagcct
     5941 gattaacagg gctaatgcgc tgcctgccga atttcgtgag ccttttggaa aaatcaaata
     6001 caagacaaaa caaactcgcc tcaataatta tttgaacatc ttttcgagca gtcggcacat
     6061 ccgtagcaac aaaagcaaaa gactatgggc taaattattc cccacacact tcaagcacat
     6121 cagggtggtt tttataaacg tttcgaaaca tctcgacaaa atcgacctcg aaatcaggca
     6181 ggacctattc gagcgaaaac gcatcaacga agatggaaat acgggaaaac atcatcataa
     6241 gaacaaaaag gatcatatcg accttgtcat ccatctcgac attatactgt taaacgacga
     6301 actcgcccag aaaatcctcg atttcctcgc cttcagatgc gaactgaacg attttcccat
     6361 ccccttcgag gaagccattt attattaatt ctctatagaa ccatttcccc gaactgtcta
     6421 catccaatgc cgtttcttga aataaaacaa cataaaacct gctaaagcca ccattacagc
     6481 agtaatgata tgaaaagcat atttgttttc ctgtaggggc aaaagaatgt tcatgccata
     6541 aacactggta atgaaggtta aaggcagaac aatggtcgaa acaagggtaa gcgtttttac
     6601 aatctcattg gtacgattgg tttgtaacga atcgaaagtt ttggaatagc cttcaatcaa
     6661 ttccgaataa tcttcgatta agtcccagat tttctgataa tagtcgagta tattgcccca
     6721 atattcttcc atgctttcgg caaagctttg tatttcaccc gattcgaatt tgttgaaaac
     6781 cgggagttgg ggcttaaaaa tcgtattcag gatgataata ttacgacgcg ttactgaaat
     6841 agattcaata gtctgcagag tctttcgttc gaaaagcatc cgcccggcct gatctacctg
     6901 atcacccacg tatcgaacca gggtataact ttctttcatt aaatattcta aaatgcgata
     6961 aagcaaagca tcactggttg cgaaatcaag atcgatatgc tgttcgattt tctgacgtgt
     7021 tttcaggaaa aactccgata cttcggaatg aatcttcccc attgtgatca cataatcctt
     7081 cccccagaat atcttttctt cgcgagtacg tacaaaattc cgtgtcttgt cgaaataggg
     7141 aaaatgtaaa ataagaaagt aatagtcgtc gtagatatcg attttgggcc gctgattcac
     7201 caaacttctg cagtcttcaa tatccagtgg atgaaaatgg aaatgctcaa gaagatagtc
     7261 gaaatcctgt tccgagggat taaggatatg atgccatttg agcgttccaa tcctgttggt
     7321 ttcgatcata accctggttt taaaaattat taatactatt gattggttta catgcccctc
     7381 aatcttttta aactttgcaa aaataaagta aattaatgaa taacggcatg ctcttggtga
     7441 atcactcaaa aagttcttat tctaactcat taataatcaa tgataaaaac taagcgatag
     7501 atcgaaccgc ataacgatgt gtaaacctct ttcgagtcca aaacgataag ctataatcaa
     7561 gatggcggcg ataaaaaggt aaaggaaagc aaatgaagtc tttttatgta aataacgttt
     7621 tttactttca gatggctggt tttgatggtt aaaaagtgga gggattacca agaacgccat
     7681 tgctactaaa ataatgatat catatagcaa aatgttctcg tgttgagggg ttcgattggg
     7741 aattttaatc caaagcatta aaagtgaacc tataaaccat ggaataatca tctggcttaa
     7801 aatgaaatag gaacggttgt agcgattgat ccttcgaggc gaattggtgg tggataaaat
     7861 atgaggggtg gaatgccagc caagatatgt caaaataccc agagctatta acgagaagaa
     7921 tattttcagt gggatcccca ggtaaagcca gttggccaca tatccaaagc cctgattggt
     7981 gatgactcca gctacgaatg ccccaaaaaa catattgaac gaatgaaacg ccagccagag
     8041 gaaaaacagc ttcattgcag gtcgctttac cttacgcatt acccatctgt accatatcaa
     8101 cccaaaaatc aaagaaacaa caggccctgc aagggtgatc atgatgatgt tgaatggagt
     8161 ccataaattt gaagcatcgc caatgggaaa catcacttca taataataca aaaccgaatc
     8221 tatcccaaag tgagccgcca tgaatataat ggcaaactga taaataaaat aggcaataat
     8281 ataggaaata ataaatacaa gtgtcgactg aatgacgtat gccgcatacc gctgaatacg
     8341 catgggtttg cgttcctctg gctgaaccgt ttcgctatcc agaaagggat cgtacggagc
     8401 cgacctaaaa ttcaaaaaaa ataagaaatt gtataaaggt tctttgatgt ggataaaaaa
     8461 atttttaaaa aacaacttaa aacccgatga tttcttaggc ttcttgtatc ttgattttcg
     8521 atgccccccc ttatgtttct tgtgatgaga atgctttctt tcagaagatt ttacccactg
     8581 gctggataat aggacctgaa cttgtggttt cgatgaaatt gaactgtcag acggctcgac
     8641 gatctccaaa ccgctttcca ctttaatttc gggctcatca agtatatttt ggtcagtttc
     8701 cgtattttta ccagagattt caggttcttc gtccaaaacc ttacggttaa aataagaaaa
     8761 gtgggaagaa tatcgccttt gtgacttatg ttttttcttt aatcccaacg atatcaatat
     8821 tctatcccaa taagttggct tgtgatgtct tcttgatgag gaatggcgtg attttttcgt
     8881 catcagaaat attgttgcaa aattttctga gtaaaactac aacattcttc attaactcat
     8941 caattaaaat aaaaataatt cttcatttcc agcacaaaat cccatacgaa aaaatgccgg
     9001 tgcataaaat gtatttacag gaacgatatt gttattacaa agaaaaagcc cttagatcgg
     9061 ccaaaacgac ctcgaatcaa taatgaaaat catctttttt gaataacaat cgattttaaa
     9121 taaattaggt tacagttaac ttcacctgac agaatttgag gccttaccca tactgtttct
     9181 gccttctctg cttgcgataa atcagaaacc gacatcttca acttcatccc gtattttgta
     9241 taacctatca acggtctgag aatatcgaca actccctctt ttccaataat aatcatagaa
     9301 gtgaaagaag cagggcattc tttgaaccta tcgagattat cgacaaaccg gtttgttcaa
     9361 tctatcaatg atatctaaaa aataataagg atgagaggaa ggagggcagt tatgattatt
     9421 gctaagtttc aaaatgaaat tttgcagaaa agttcagtaa attcaaacac ctcattcggt
     9481 tttaatcagg ttgtcgtggt tttgattatt gcatcatagc attacctcat taccttctct
     9541 aaataatcac tcttatcacc cttttgaggc ctactcccat cacctacaaa cgcgaattcc
     9601 ttattaaatt tcttcttctg acgtcaaatt ctaaaaggtt ctgcctattg gcgagaaaag
     9661 aattgcaggc tgggtttgaa ccatttatgt aagatgaaaa atcgaaatac gagaaaaaaa
     9721 gcgttatgtc gacttactta agttaatttt gcacctcatt tgtggaaagt atgatgcctt
     9781 ggggagaact taccgcaatg ctcactgcta ttttctggac ggccactgcc atggcattcg
     9841 aagtggccag caaaagagtg ggctccctta gggtgaatac tttgcggctt cccatggcct
     9901 tcatctattt ctttatcttt tgctggatct atcgcggaca tcctctcccc atcgatgcac
     9961 cattgcatgc atggatatgg ctcatcctct cgggtttgat tggtttcgtc ttcggagatt
    10021 atttcctctt taaatccttt accatcatgg gatcaagaac taccatgctc gtccaaactc
    10081 tcgtaccgcc aattacagct ctggtgggtt ttatcctgct ttccgaaaaa ctttcgctaa
    10141 aacatattgc aggtatgacc ctcacactga gtggaatctt tctcgtggtg cttaccacca
    10201 acaatggcaa gttaggtttg aaaattcctc gccgtggact cttatatgct atttttgcag
    10261 ctctcggcca atcaattgga ctggttctga gtaagttcgg catgagagat tacgatgctt
    10321 tcgcctcttc gcaaataaga gttatggccg gtgcattggg ttttcccatt ctttcgtttg
    10381 cattgcatca gtcaaattcc ttgaaaaaaa cctttcacga taaggtagcc atgaaaagca
    10441 tcttgatagg ctctattttt ggacctttct tgggcgtatc attttctctc atcgctatcc
    10501 aacataccaa aaccggaatt gcatctaccc tcatggccct tacgccagtc atgatccttc
    10561 tcccttcggc catcctgttt caccaaaaaa tcgtctggaa agaaattgtc ggagctatta
    10621 tcagcgtgag cggtgcttca ttgttcttcc tataaaaagc aactgattct attttccttt
    10681 tgtctttatt cctaaatatt aaatcattca tcaagtcagc aaagattttt aaaatatcac
    10741 tcaaattaag aagcattcat acattcgttt gctattcatc tatgtattct ttgaaatcaa
    10801 aataagaaag attttgtgat cgatggcagc tttatcttca atttatctta agtgtattgt
    10861 tgtccttaat cgaaaatttc aggcaacagt ctaatggctc tttcgatgaa tggatattcg
    10921 atgtagttgg tcttatctaa aaaatcaaaa atatattgct gtgtgggact tggttccaaa
    10981 ctcgcctaag cttttctcat atctttcttg aagcaccgca agacaagaaa tgagaacatt
    11041 ctttatgcca acatgcaaac aaaggcatat tttcagggtt attctaaaaa gaatgcgtct
    11101 gctggataat aaaatgtcga ccaaagaaag ctgacacatt ttttcgaaag gtcttcagta
    11161 ctctggtatt attatactcg aaaaagttct ttatatttgc agtctgttct ttgcatgaag
    11221 aggcttatcg agaaaggtgg agggaatggc cctgtgaaac cttggcaacc tgccctcgtg
    11281 gtaaggtgcc aactcctgcc ctaaaactgg gaaagataag ttcaataagg taatgagtaa
    11341 ttctatacga ctcaaaactc cctgttgggc atttacccag tagggagttt ttttatgcac
    11401 atctccacat ccttctttat attagatagc accttcgtga gaaaacagat ctcgacttta
    11461 aatcaataaa attttaaaga cagcatccaa aatacattat taaatcttcg aatcgaaacc
    11521 aatgggaaaa acaatctacg aaatcattca ggagagagtg ctggtaatgg atggagccat
    11581 gggaactatg atccagaggt atcaactgaa agaagaggat ttcaggggag aactcctgaa
    11641 acatcatcct tttgatctga aaggagataa cgacatttta tgcctcactc agcccgacct
    11701 gatcaaagaa atatat
//
LOCUS       DAOORU010000064        11508 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_6996_length_11508_cov_5.747577, whole genome shotgun sequence.
ACCESSION   DAOORU010000064 DAOORU010000000
VERSION     DAOORU010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11508
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6996_length_11508_cov_5.747577"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..2197)
                     /locus_tag="PLF20_05475"
     CDS             complement(197..2197)
                     /locus_tag="PLF20_05475"
                     /inference="COORDINATES: protein motif:HMM:NF025486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80489.1"
                     /translation="MQNSFPLMKLIAGFASSIILSLFSTSLFSQQSLWITPRDSTEIL
                     EFCNRIQSLETISLERIDTTTSGIHEYDPFKKGFYLGLGNIGQPVKSLLPEFKPNKGF
                     QFGRDAMDVYRFTPQNLPFYQSSSVFSEVRYVMGPDKENNIHAKFNRNLYRGLNIGLN
                     YRLIHSTGPYTRQLTDLDNIGVDVRYFSPSKHYGVMAQFIRNVFDFQQNGGIQNENEF
                     EQNRESRRPVIPVKLSSARTKEIGNVYTITQFYEPHWGRAVKIPAQPSVYDSLMMRID
                     TLHLPNDSSGQNPDIIAQAGENQSRLFTLGRFSHTFSYSKDAYAYSDDSPKSGFYPEI
                     LKDSTKTYDSITIYRIENELSWTNSLYLYPTKFPIKLRLALRYLIAGYHFDSLEIKNF
                     HQWIPTVDLRIDFPAGIRLQGNAFYVNGDYNGGDLGIEGSLSKFFGKKPLWSLAAEAS
                     FSSSHPDYFYVSYRGNHQQWENNFQREEILRLGGSISGPHTLVKPEFILLNNLVYLAK
                     TAKPMQRNGSFFLWRLYLKQGLRFRHFHMDATVVLHKLSDTLSLRLPSIMAKGSLSYV
                     GYMFKDALFIEPGISASWYSKFYADAYMPSLNAFYLQSEKQIGNYFVADIFLNLKVAR
                     ARLFLRYRHFHSAWTGYNYWGAPGYPLPDAGINFGVIWPFYD"
     gene            complement(2204..3166)
                     /locus_tag="PLF20_05480"
     CDS             complement(2204..3166)
                     /locus_tag="PLF20_05480"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOX80490.1"
                     /translation="MDDRVLCLNASPSGCILSGNSKISTFALWLWLAEFFTILNFSAI
                     FESKDTMWQKITSVSNPHIRELVRLKNKPAERKNRGWMIVEGLRELRMALGYPMKAIE
                     LIVCRNICSEEKLKEIEPYFSSVLKTEVGLEVFGRLAYRENSDGFLAIFEADTPRLDS
                     LNLDDDPLVIIIESVEKPGNLGAILRMADAAGCDAIIVSDPRTDIFNPNVIRASLGTV
                     FTVPLAVAESKEVMKWLEQRHITSYAAVIVEDALPYTEMDFTASTAFVLGTEAEGLSP
                     AWLRFCRHKIMIPMRGRADSLNVSIAAAILTFEAVRQRNQPKKA"
     gene            3345..4244
                     /locus_tag="PLF20_05485"
     CDS             3345..4244
                     /locus_tag="PLF20_05485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX80491.1"
                     /translation="MELIETKCWRDYELLDTGKSQKLERFGPYVLVRPEPKAIWDKHL
                     SDEEWEGKSQAVFRRTYNSSRNDKEESGEWYKKKDMPDQWVIEFPMDHFYLKMRLGLT
                     PFRHVGIFPEQATNWEYIYETISMMKIDKPKVLNLFAYTGAASIVALAAGAEVVHVEA
                     LKQLITWTNENMTLNQLSGIRWMLEDVVKFVKREIRRGHKYHGIIMDPPAYGRGAEGE
                     KWILEEGINDLLKDARQLMFSQNRFFVLNLYAKGISPLITENLFDDIFGEVKGKECGE
                     LFIQDHYGRRLPYGSLLRFREIG"
     gene            4564..8283
                     /locus_tag="PLF20_05490"
     CDS             4564..8283
                     /locus_tag="PLF20_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80492.1"
                     /translation="MKQLKQFYTIFLVLLLGFHLQVWSQVNISAGNTITENFDGMGTS
                     ATANLPNGWKVDKNTTVRSVGTYSAAGTATELRAGNNMASNAQNGIYNYAAGVPDSAT
                     DRAVGGISSGSASKSVNVYVQLTNNGSTAIDNLTISYNVEKYRNGTNSAGFTIQMYYS
                     TDGSTWTSAGDDFKSSFAADADNNGFTSAPGATVNVTSKTLNQSIAVGSSLYLAWNYS
                     VTSGTTTSFAQALGIDDVSITANGGTSPSISVNPSSLSGFSYVLGAGPSSEQSFTVSG
                     QNLTNDITLTAPTNYEVSQTSGSGFTNTINLPQTGGSVSSTSIYVRLKSGLSVGTYDE
                     SITASSTGATSQEISCSGVVFATEPSNHVSNFTANATSSSEIQLTWTDADASNYLIKG
                     SSTSFAAIVAPVDGSAEADGELVKNVASGVQSHSFTGLSSNQTYYFKIYPYNGTESTI
                     NYKTSPDPPEASVTTQAQAATTYTWIGDDNASWLEATNWSPTRTSPQDNDILIFNSGT
                     KTITAVPSQTIGQLKVTDGAKITLKASEGITLTINGDEGDDLSVASGCELNISGSNAL
                     TISLQTSATGNIAGSMTFSGGGHKLTASAASAIIFSGTFTAGSGFSGNPFGTTTHNSV
                     VFQSGSTYIYMAGGNPFGAGQPNSVVVFQTGSLYSHRGSGTPSFSGRTYADFELDYNG
                     GTISPSGTSAVSIDNLTIKQGTMNFNTTGIPGHSIKGNINIYTGATLNFNPSSAGTVL
                     LNGTSLQTISGEGTLSASSNSTIQVDNTNGVKLNKTATLYNMTVSSGAIFELSSSAAL
                     TLSGNLTNSGNFSLQSESTGTASFILNGNYSGSGTFEMERYVAGADWNTWDDGWHDIS
                     SPVQDQEISTFTTGIYDFYGWDETQNLWINYKDGSFIPWNDNSSLFVEGRGYLIAYDE
                     TQTDKKFTGTPNNSNITISNLSYTSDKGNGWHLLGNPYPSALKWNDGNWTLTDIGGVA
                     KIWNSSNKSYEDVNSNGIIPATQGFFVQVSASTSLTIPAASRVHNSTNWYKSSDDASS
                     IVLAASPADNSSTQKTYIRTEEGTTEGFEFSSDSRFLAGYAPKFYCMAGGEKLSTQSL
                     PTIYNGLELNYGFEKLENVNQYRIQLIENPYSATLYLKDLKTGSIQDMSQNPVYEFTA
                     SEGDPALRFKLSFGSVGIDEPINNNYRLYYSNGIMYMLQMDGHKQIEVYQLNGQLIRK
                     LNSTENFVNLQLAKGIYLVRVINANQAYVQKMIVQ"
     gene            8303..8539
                     /locus_tag="PLF20_05495"
     CDS             8303..8539
                     /locus_tag="PLF20_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80493.1"
                     /translation="MKRYIFRLMLFAAILVASLSVYSQPQNPPPPPAGGHGQSTNQSR
                     GGGAPLGTGIGIMLALGGAYGAKKVYDARKNSKE"
     gene            complement(9210..10466)
                     /locus_tag="PLF20_05500"
     CDS             complement(9210..10466)
                     /locus_tag="PLF20_05500"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOX80494.1"
                     /translation="MISKLRIINDPVYGFIPIPNELIFSLIEHSFFQRMRRIKQLGLT
                     HLVYPGALHTRFHHSLGSMYLMREAIQVLRERGIEISEQESEGALIAILLHDLGHGPF
                     SHSLESAIVRNIGHEHLTGLFLNELNKEYDDALGIGLSMFQGDYERKFFHQLLSGQLD
                     MDRLDYLRRDSFFTGVSEGQIGSERIIKMLNVVDDNLVIEEKGRLSLEHFLSARRIMY
                     WQVYFHKTVLAAETLLIKILNRAKYLAAQGEKLFATTHFLRFLREDFFLCDFQEDPTL
                     LRSFARIDDFDVFSSIKAWTEHPDPILSCLSNYLVDRRLFRIYFSDKPFDHNHLEIAK
                     NRVEKILNLKKGEGDWFIGTGEVCVMAYDQETGPILVKTAQGNIEELISAISSPNNLI
                     PLQPEVKYFLCLPKTVKLNPDSEEEE"
     gene            10722..>11508
                     /locus_tag="PLF20_05505"
     CDS             10722..>11508
                     /locus_tag="PLF20_05505"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOX80495.1"
                     /translation="MKSLLKIFVCAIMAIMVSSCVSQKKYTETEQKRRQCEEELIAQK
                     SQNNDLIADNTELKVRLERLNKNFQRLINDTIRIGQNLRDFQADYERLKFSYNELLDI
                     AGKTEAGNKAEIQRIMAELQSSQEKLIHKQDSLQALEAELAVKQKHLNELQKILNQKD
                     SIVNALRQKVSQALLGFEGKGLSIEIRNGKVYVSLEESLLFRSGSWEVNDRGISALQK
                     LAEVLASNPDINVLIEGHTDNVPYRGSGQVKDNWDLSVMRATAI"
BASE COUNT         3512 a         2313 c         2335 g         3348 t
ORIGIN      
        1 atggccatca taggtggcga acgggatccg gacaaacttt gttcacttgt tcacgattgc
       61 atcttcagaa ctcctgattc gacattgaat caagagttct gatctttatc gagagtcgaa
      121 atatctatcc gtttttttat aaaattttta ggttaggatt ttaaaaaata cccgaatcat
      181 cggcatggaa agttcattaa tcgtagaaag gccatatcac gccaaagtta atgcctgcat
      241 cgggcaaggg atatcccgga gctccccagt aattatagcc cgtccatgcg ctgtgaaaat
      301 gccggtatct cagaaataat ctggcgcgag ctacttttag attcaagaaa atgtcggcca
      361 cgaaataatt cccaatttgt ttttccgact gcagatagaa tgcattgagg gaaggcatgt
      421 aggcatcggc ataaaattta gaataccacg aagccgaaat accgggttcg ataaataaag
      481 cgtccttaaa catgtatccc acataactga gtgaaccttt tgccatgatc gaaggcaaac
      541 gaaggctgag ggtatcgctc agtttgtgca aaaccacggt agcatccatg tgaaaatgcc
      601 tgaaccgtaa accttgtttc agatataacc gccataagaa aaatgaaccg ttgcgttgca
      661 tgggtttagc cgtttttgcc agataaacca aattgttgag caatataaat tctggtttaa
      721 cgagcgtgtg gggtccactg atcgaccccc ccaaacgtaa gatttcttct ctctggaaat
      781 tattttccca ttgctgatga tttccccggt aagaaacata gaaataatcg gggtgagatg
      841 aactgaaaga tgcttcggct gcaagcgacc ataatggttt tttgccgaaa aatttggata
      901 ggcttccttc aatccccaga tcgcctccat tataatcgcc attcacatag aatgcgttgc
      961 cctgcaaccg aattccggcc ggaaaatcaa ttctcagatc caccgtcggt atccactgat
     1021 gaaaattctt tatttcaaga gaatcgaaat gataaccagc aataagatat cgaagagcca
     1081 atcgtaattt tatgggaaat ttcgtaggat acaaataaag agagttagtc cacgatagtt
     1141 cattctctat tcgataaata gtaatcgaat cataggtttt ggtcgaatct ttcaatatct
     1201 ctggataaaa tccgctttta ggagaatcgt ccgaataagc ataggcatct tttgaatacg
     1261 aaaatgtatg cgaaaatctt cccaaagtaa acaagcggga ctgattttct ccggcttggg
     1321 caatgatatc ggggttctgc ccactactat cgttcggaag gtggagtgta tcgattcgca
     1381 tcataagaga atcataaacc gatggttgtg caggtatttt cacagctctt ccccaatgag
     1441 gttcataaaa ctgtgtaatg gtataaacat ttccgatttc tttggtccga gcagaactaa
     1501 gcttgactgg gattactggt cgtcggcttt cgcggttttg ctcaaattca ttctcattct
     1561 gaatgcctcc gttttgctgg aaatcgaata cattgcgaat aaattgggcc attacgccgt
     1621 aatgcttcga aggagaaaaa tatcgaacat cgacgccaat gttatcgagg tcggtcagct
     1681 gacgggtata ggggcctgtg gaatgaatca gcctgtaatt taatcctata tttaatcctc
     1741 ggtaaagatt ccggttgaat tttgcatgaa tgttgttttc cttgtcaggc cccattacat
     1801 atcgaacttc cgaaaacacg gaagaagact gataaaaagg tagattttgt ggcgtgaagc
     1861 gataaacatc catggcatcg cgaccaaatt gaaatccctt attgggcttg aactcgggaa
     1921 gcaatgactt tacgggttgg ccaatatttc ctaaccccag ataaaaacct ttcttaaacg
     1981 gatcgtattc atgaatgcca ctcgtggttg tatctatacg ttcgagagaa atggtctcga
     2041 gactctgaat acgattgcaa aattcgagga tttcagtaga atcccttgga gtaatccata
     2101 gcgattgctg agaaaaaaga gatgtagaaa acaggctcag aattatactc gaagcgaatc
     2161 ctgcaataag tttcattaaa gggaatgaat tctgcataag cgttcaggct tttttgggtt
     2221 gatttctttg cctgactgct tcaaaagtaa gaatagcagc agcgatcgat acattgaggg
     2281 aatctgctct tcctctcatg ggaatcatga tcttatgccg gcaaaatcga agccatgcag
     2341 gcgaaagtcc ttcggcttcg gtgcctaaga caaaagccgt acttgccgta aaatccattt
     2401 cggtgtacgg aagtgcatcc tccactataa cagccgcata agaagtgata tgtcgttgtt
     2461 cgagccattt cattacttcc ttcgattcag caacggctaa aggaacggta aaaaccgtcc
     2521 caagactggc acgaattaca tttggattga aaatatcggt gcgaggatcg ctgacgatga
     2581 ttgcatcaca tccggcggca tcggccatac gaagaattgc acctaaattt ccgggctttt
     2641 cgacactctc gatgatgata accagaggat catcatcgag attcaatgaa tccaagcggg
     2701 gagtatctgc ctcgaatata gccagaaacc catcactgtt ttcacgatag gccaaacgcc
     2761 caaaaacctc taaaccgact tccgttttca gtactgaaga aaaataaggt tctatctctt
     2821 ttaatttctc ttcagaacaa atattgcggc aaacgataag ttctatggcc ttcatgggat
     2881 aacccagtgc catgcgtagc tccctcaaac cttctactat catccaaccc ctgttcttcc
     2941 tttccgcagg cttgttctta agcctcacca gttcccgtat atgcggattg gaaacactcg
     3001 taattttttg ccacattgtg tctttcgatt cgaagattgc tgagaaattg agaattgtaa
     3061 agaattcggc caaccacagc cagagagcaa aggtagaaat tttagaattg cccgaaagaa
     3121 tacatcctga gggtgatgca ttcaaacaca aaaccctgtc atccattttt cgaataacca
     3181 gaattgcctt ctttgaattt tattgcttga tcccaagatg attggtttat ttcgaacatt
     3241 ggctcgaaaa ccatagttct gaaaatgact catacccttt agcatccccg ataaacattt
     3301 ggcattttta atacatttgc accgcatgaa ttattcctga attaatggaa ctcatcgaga
     3361 cgaagtgctg gagagattat gaacttctgg atacgggcaa atcgcaaaaa ctggaacgat
     3421 tcgggcctta tgtgcttgtt aggcctgagc ctaaagcaat atgggacaaa catttaagcg
     3481 atgaagaatg ggaagggaaa tcgcaggcag tttttagacg cacttataat tcgagccgca
     3541 acgacaagga agaatccgga gaatggtaca agaagaaaga catgcccgac cagtgggtga
     3601 tcgaatttcc gatggatcat ttctatctga agatgcgctt gggtttgact ccctttaggc
     3661 atgtaggcat attccccgaa caggctacca attgggaata tatctatgaa accatttcga
     3721 tgatgaagat cgacaagcca aaagtcctga atctgtttgc ctatacaggg gcggcttcga
     3781 ttgtagcact ggcagcaggt gccgaggtag ttcacgtaga agctctcaag caactgatta
     3841 cttggactaa cgaaaatatg actctcaatc agctcagtgg aataagatgg atgctggaag
     3901 atgtggtaaa attcgtgaaa cgtgagattc gccgtggaca taagtatcat ggcatcatca
     3961 tggacccacc ggcttatggg cgtggagccg aaggagaaaa atggattctg gaagaaggaa
     4021 tcaatgactt attgaaagac gcgagacaac tgatgttttc acagaaccgt ttttttgttc
     4081 ttaaccttta tgcaaaagga atttcaccat tgattaccga aaacctcttc gacgacatat
     4141 ttggagaagt gaagggaaaa gaatgcggcg aactgtttat tcaggatcat tatggccgcc
     4201 gtttgcctta tggaagttta ttgagattca gagagattgg ataaaaatag ttctccatag
     4261 aaggttaggg attgatgggt taaaagttta taaagtggag aaatggaaac tagaagacga
     4321 ggggacggag agagtttgat taaatgggaa attatgaata ttttccctaa atattgaaat
     4381 tagacaatta aatttggata aaattaacat taaattttca agaatcttat tttattttaa
     4441 aaaatttatt tatttttgtt cacgctctat aaaagtttag tattttgaat tttgtaaaga
     4501 aaagtttagt attattttaa tcaaagacat gcaacaactt gtaacccttt aaattacatt
     4561 tttatgaaac aattaaaaca attctacaca atttttctcg ttttactttt gggcttccat
     4621 cttcaggtat ggagccaggt aaacatttcg gcgggtaaca ccattaccga gaacttcgat
     4681 ggtatgggta catctgccac tgctaatctt cctaatggat ggaaggtaga taaaaatact
     4741 acagtacgtt ctgttggaac ctattccgct gctggaactg caacagaact gagagcaggg
     4801 aataatatgg ctagtaatgc tcagaatggc atttataatt atgcggcagg ggttccagat
     4861 tcggccaccg acagggcagt gggaggaatt tcttccggca gtgcctctaa aagtgtgaat
     4921 gtatatgtac agctcaccaa caatggcagc actgccatcg acaaccttac aatcagttac
     4981 aatgtggaaa aatatcggaa tggtactaat tccgctggat ttaccattca aatgtattat
     5041 tccactgatg gaagtacatg gacaagtgca ggagatgatt ttaagtcaag tttcgcagct
     5101 gatgctgata ataatggttt tacttctgca ccaggagcaa cagtaaatgt taccagcaaa
     5161 actttgaatc aatctattgc cgttggaagt agcttgtact tggcatggaa ctattctgtt
     5221 acttctggaa ctacaacatc ttttgcacag gctttgggta tagatgatgt ttccatcaca
     5281 gccaatggtg gaacttcacc cagtatttcg gtaaatcctt cttctctgag tggttttagt
     5341 tatgtgttgg gtgctggtcc ttcatcagaa caatcgttta ccgtgagtgg ccagaatttg
     5401 actaacgata tcaccttaac tgcaccaaca aattatgagg tttctcaaac ttcgggaagt
     5461 ggctttacca ataccattaa tttgccacag accggtggaa gcgttagttc tacttcaatt
     5521 tatgttcgcc tgaaaagtgg gctttccgtt ggaacttatg atgaaagcat taccgcttct
     5581 tcaacgggag ctacttcaca ggaaataagc tgttcgggtg tcgttttcgc cactgagcct
     5641 tccaatcatg tttccaattt tacggcaaat gcaacttctt cgtccgaaat tcaactcaca
     5701 tggacagatg ccgatgcctc aaattattta attaaaggta gttctacaag ttttgctgct
     5761 atcgtagctc ctgtggatgg gtccgcagaa gccgacggtg aattggtaaa aaatgtggct
     5821 tcaggagttc agtctcatag ctttacagga ttaagttcca atcagaccta ttattttaaa
     5881 atttacccat acaatggtac agaaagtacg ataaattaca aaacttcacc cgatcctccc
     5941 gaagcaagtg ttaccactca agctcaagct gccactacct atacttggat aggtgatgat
     6001 aatgcttcgt ggctggaagc cactaattgg agcccaacgc ggacttctcc ccaagataac
     6061 gatatcttga tatttaatag tggaaccaaa acaattacgg cagtcccttc gcaaaccatc
     6121 ggccaactaa aagttacgga tggcgcaaaa attaccttaa aggcttcaga aggtattacc
     6181 cttactatta atggtgacga aggtgatgac ctttctgtgg cttctggttg tgaacttaat
     6241 atttccggtt cgaatgccct gaccatttct ctgcaaacaa gtgctaccgg aaatatagcc
     6301 ggctcaatga cattttcggg aggtggtcat aaattgacgg cttctgctgc cagtgcaatc
     6361 attttttcgg gaacttttac agcgggtagt ggttttagtg ggaatccatt tggaacaaca
     6421 actcacaatt ctgtggtttt ccaaagtggt tcgacttata tttatatggc aggaggcaat
     6481 ccttttgggg cgggtcagcc taatagtgta gttgtatttc agacagggag tttatactct
     6541 cataggggaa gcggtacacc atcattttca ggcagaacct atgccgattt tgagttggat
     6601 tataatggtg gaactatttc tccaagtggt acaagtgctg tatctattga taacctaacc
     6661 attaagcaag gcaccatgaa ttttaatacg acaggcatac caggtcattc tatcaagggg
     6721 aatattaata tatatacagg agctactttg aatttcaatc cttcttcagc tggcacggtt
     6781 cttcttaatg gaacttcctt gcaaaccatt tcgggagaag gtaccctttc agcttccagt
     6841 aattctacta tccaagtaga caatacaaat ggagtaaaac taaataaaac tgccacgctt
     6901 tacaatatga cagtttcgtc gggtgccatt tttgaattat cttcttccgc tgccttaacg
     6961 ctcagcggta acctcacaaa cagtggcaat ttttctctac aaagtgaatc aacaggaaca
     7021 gcttctttta ttttaaatgg caactactca ggaagtggta ctttcgaaat ggaacgctat
     7081 gtggccggtg ccgattggaa tacttgggat gatggttggc acgacatcag ctctcccgtt
     7141 caagaccaag aaatttctac tttcactact ggtatttatg atttttatgg atgggatgaa
     7201 acacagaatt tgtggataaa ttacaaggat ggcagtttta ttccttggaa cgataacagc
     7261 agtctctttg tcgaaggtcg tggatatcta attgcttatg atgaaactca aaccgataag
     7321 aaattcacgg gtacgcccaa caatagtaat attactattt ctaacctaag ttatacttct
     7381 gataaaggga atggatggca tctattagga aatccttatc caagcgcttt aaaatggaac
     7441 gatgggaatt ggacacttac agatattggt ggagtagcaa aaatttggaa ttctagcaat
     7501 aaatcttatg aagatgtcaa ttcaaatggc ataatacctg ccacccaagg attttttgta
     7561 caggtaagtg cttcaacttc tctcactata ccagcagctt cacgggttca caattcaacc
     7621 aactggtaca aatcgtcgga tgatgcttcg agtattgttt tagccgcttc gccagccgat
     7681 aattcgtcaa cccaaaaaac ctatataaga acagaagaag gaactactga aggtttcgaa
     7741 ttttcaagcg acagccgttt cttggcgggt tatgcaccga agttttattg catggctgga
     7801 ggcgaaaagc tttctacgca atccttaccc acaatctata atggtctcga gctaaattat
     7861 ggttttgaga aattggaaaa tgtaaatcaa tatcgtatcc aactcatcga aaatccttat
     7921 tctgccacac tttacctgaa agatttaaaa actggaagca tacaagatat gagtcaaaac
     7981 ccggtttatg aatttacagc ttcagaaggc gatcctgcgc tgcgtttcaa attaagcttt
     8041 ggaagcgtag gtatagatga accgattaat aataattacc ggctttacta ttccaatggt
     8101 ataatgtata tgctgcaaat ggacggacat aaacaaatcg aggtttatca actcaatggg
     8161 caattgataa gaaaactcaa ttctaccgaa aattttgtca atcttcagct tgccaaagga
     8221 atctatctgg taagagtaat taatgccaat caggcttatg tacaaaaaat gattgttcaa
     8281 taatatataa atctataaac ttatgaaaag atacattttt cgattgatgt tattcgcagc
     8341 tattttagtt gcttcccttt cggtttattc tcaaccacaa aatcctcctc ctcctcctgc
     8401 aggtggtcat ggacaatcta ctaatcagtc aagaggtggt ggtgctcctt tagggacggg
     8461 tattggtata atgcttgcct tagggggtgc ctatggagct aaaaaagttt atgacgctcg
     8521 caaaaattca aaggagtaaa ccttacataa atctcttttc atggctaaag aggctgctct
     8581 catcttggga gcggcctctt tcgtctttat gatcatgatt gtataagaat cttattttta
     8641 tttcagattt attttagaat tcctgattca aaaatagatc ggtttatcgt gtttatgcaa
     8701 acaagcgcat agccttcaca aaactgaata cttaagtgca ttgagttgtt gaggtgaact
     8761 ccttttgtca gaatctcttg atgctatttt aaaatttccc tttttgataa gataataaat
     8821 ggcagcctca ccaatgccag gttctttcgc tgtgctgtat tacgaacact ccttattcat
     8881 cctcgcctgt gcttcccgct tcatcgattc tgtaaacttg taacactgcc cacgatgctt
     8941 tgcgcgatta acaaacccag tctgcacctt tctctttcag tgatttgaca caactctgaa
     9001 cattccttac gtgaatgccc agggggctgt ttaagaaaag gcaatcgaag tcgtacgtcc
     9061 gaaaattttt catcggggaa atgcccattc gaatcatcat catattttta aaaatattga
     9121 aaataaaaat aaagagtcga aagtttcaaa tttatctccc tgcctatata tttcttcctc
     9181 gccttgaatc tcttccattc aaaaaatctt cattcttctt cttcagaatc agggttcaat
     9241 ttcactgttt taggcaagca taaaaaatac tttacttcgg gttgaagagg tattaaatta
     9301 ttgggtgatg aaatcgcaga aattaattcc tcgatatttc cctgtgctgt ttttaccaaa
     9361 ataggcccag tttcctgatc ataagccatg acacatacct cacctgttcc tataaaccaa
     9421 tctccttctc cttttttgag attcaatatt ttttccactc tatttttagc aatctctaaa
     9481 tgattatgat cgaagggttt atccgaaaaa taaattctaa aaaggcggcg gtcgaccaga
     9541 taattgctca aacaggataa gatagggtcg gggtgctcag tccaggcttt tatcgaggaa
     9601 aacacatcga aatcatcgat gcgtgcaaaa gaccttagca gtgtaggatc ttcctggaaa
     9661 tcacatagga agaaatcttc tcgaagaaat cgtaagaaat gcgttgtagc aaacagtttc
     9721 tcgccctgag ctgctaaata cttggctcta tttaaaattt taatgagtag ggtttcggca
     9781 gcaagcacgg ttttgtgaaa atatacctgc caatacataa tacgccttgc cgaaagaaaa
     9841 tgctcgagcg acaaccggcc tttttcttcg attaccaggt tatcatccac tacatttagc
     9901 attttaataa tacgttccga gccaatttga ccttccgata ctcccgtaaa aaaactatcc
     9961 cttcgaagat aatccagacg atccatatcg agttgccccg ataagagctg atggaaaaat
    10021 ttcctctcat aatcgccttg aaacatggat aaacctattc ccaatgcatc atcatattct
    10081 ttattcagct cgttcaggaa aaggcctgtc aaatgttcat gacctatatt cctaacaatt
    10141 gcactctcca atgaatgcga aaatgggcca tgacccaaat cgtgcaacag aattgctatc
    10201 aatgctcctt cgctttcctg ctcgctgatt tctatcccac gctcacgtaa aacctgaatg
    10261 gcttccctca tcagatacat cgaacccagt gaatgatgaa aacgcgtatg cagcgctcct
    10321 ggataaacca aatgcgtaag ccccaactgt tttatccttc tcatacgctg aaaaaacgaa
    10381 tgctcgatca ggctaaaaat tagctcgtta ggtataggga taaacccata aaccgggtca
    10441 ttgataattc taagcttact aatcaatccc tttgctattt atctttatgc aaacataatc
    10501 aatcattcta ttttccaata atatcatcag aatgataatt gaacgaaagc tatttatttt
    10561 tgcaagtatg aaacgaagat cccgttctat atgataactg tcttatatat actacaagat
    10621 ggaatggggg ttatttaaat cgcgcagtca aaaaatctct tataaagacg atttgtaata
    10681 gtttaacgtt atttttctga atcaaataaa atcgacacca gatgaaaagt ttactgaaaa
    10741 tcttcgtttg tgcaataatg gccatcatgg ttagttcatg tgtttcccag aaaaaatata
    10801 ccgaaaccga acaaaagcgg cgccaatgcg aagaggagct tattgcccaa aaaagtcaaa
    10861 acaatgattt gattgccgat aataccgaac tcaaagtacg cctcgaaaga ctaaataaga
    10921 actttcagcg tttaatcaac gacactatcc gcatagggca gaatcttcgc gattttcaag
    10981 ctgattatga aagattgaaa ttttcgtaca acgaattgct cgatattgcg gggaagacgg
    11041 aagccggcaa taaagccgag attcagagaa ttatggctga attgcagtca tctcaggaaa
    11101 aattgattca taaacaagat tcattacaag cacttgaagc agaattggct gttaagcaaa
    11161 agcatttgaa tgagttgcag aagattctaa atcaaaaaga ttccattgtc aatgcattga
    11221 ggcaaaaagt ctcgcaagct ttgttaggtt tcgagggcaa aggcttgagc atcgaaattc
    11281 gtaatggtaa ggtttatgtt tcgctggaag aaagcctgct tttcaggagt ggttcatggg
    11341 aggtaaacga cagagggatt tctgctcttc aaaaactggc tgaggttctt gcctctaatc
    11401 ccgatatcaa tgttcttatt gaaggccata cggataatgt tccctataga ggaagtggac
    11461 aggtaaagga caactgggat ctcagcgtaa tgagggcaac agctatcg
//
LOCUS       DAOORU010000065        11430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7061_length_11430_cov_5.617582, whole genome shotgun sequence.
ACCESSION   DAOORU010000065 DAOORU010000000
VERSION     DAOORU010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11430
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7061_length_11430_cov_5.617582"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="PLF20_05510"
     CDS             complement(<1..309)
                     /locus_tag="PLF20_05510"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOX80496.1"
                     /translation="MRKLYMLTLLLFGLSFGMMAQQVITIKGQVKNKKDGTTIPGVTV
                     LLKGTTTGTVTDIDGNYMLNAPNNGILVFSFVGMKVIEEPIQGRSRIDIEMEPMISQL
                     E"
     gene            734..1468
                     /locus_tag="PLF20_05515"
     CDS             734..1468
                     /locus_tag="PLF20_05515"
                     /inference="COORDINATES: protein motif:HMM:NF040269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutT"
                     /protein_id="HOX80497.1"
                     /translation="MDPNVIPNVSVDCVVIGYDLQDISILLIEREMVYNGKKYIDLKL
                     PGDLVRWDEDIDSSAYRILFDLSGVQISNLKQLQAFGEVDRLKKEPRDMEWLRAIDHP
                     EERVITVAYYTLVSLDLIHSQKFKPNPNARWVPISKIGKLAFDHNRIINKALDTLRQE
                     IKQTPVAFDLLPRKFTLSQMQKLYEVVMGTLFDKRNFRKKIAQIPCIVPIKEKQVNVP
                     HKPARYYMFSRDVYEVTRKDNFDFSV"
     gene            complement(1487..2848)
                     /locus_tag="PLF20_05520"
     CDS             complement(1487..2848)
                     /locus_tag="PLF20_05520"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOX80498.1"
                     /translation="MKKPIGLFMIILLIFSCTNRISKQEFSLEDLQKRTFRYFWVSAD
                     PVTNLVPDRWPTPSFSSIAAQGFGYPAWIIGIENGWVTREDVALKVKNSLKFLYNLPQ
                     GAQTSKISGYKGFYYHFLDMKTGSRFKKVELSSIDTGLLMAGILTCMSYFDRDNSVEA
                     EIRQLADSLYRRVEWDWMFDSTQYALSMGWHPENGFIPSWWSGYNEAMILMILAIASP
                     THPIPANAWQRWCSTYIWAKFQGYEHVNFGPLFGHQYSHLFIDFKGLQDSFMLSKGID
                     YFENSRRATLANRAYCISNPLGFSHYNDSIWGLTACDGPKDTVIQSDERQIRIHSYSA
                     RGAAANYLVDDGTIAPTAAGGSIPFAPEICIPALRAMYTLYGDKIYGMYGFKDAFNPF
                     YKDQNNEFGWYDIDYLGIDQGPILIMIQNYYNQLIWNILRKNSYIREGLIKAGFKGGW
                     LES"
     gene            2987..4009
                     /gene="tsaD"
                     /locus_tag="PLF20_05525"
     CDS             2987..4009
                     /gene="tsaD"
                     /locus_tag="PLF20_05525"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOX80499.1"
                     /translation="MSIYILGIESSCDDTSAAVLRDDLLLSNVIANQEIHYKFGGVVP
                     ELASRAHQQSIIPVVDQAIWQAKLTPQQIQLVAFTQGPGLLGSLLVGVSFAKAFALAL
                     GIPMTGCDHMQAHILSHFIRKPGCKHRVPSFPFLCLTVSGGHTQIVKVDDYDKMKIMG
                     RTIDDAAGETFDKAAKIMGLPYPGGPVIDRHARKGNPMAFSFAKPLIAGLDFSFSGLK
                     TSFLYFLRDRLKENPSFISEYLDDLCASIQKSIVDILMEKLKKAVEITGITEVAIAGG
                     VSANSGLREALIRAGQEGAWNVYIPEFAFTTDNAAMIAISGYLKYLKGDYSMQDAIPY
                     ARSRMQ"
     gene            complement(4053..6509)
                     /gene="lon"
                     /locus_tag="PLF20_05530"
     CDS             complement(4053..6509)
                     /gene="lon"
                     /locus_tag="PLF20_05530"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008761520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOX80500.1"
                     /translation="MKIYDTPYLLSDLIEGNVEFIPLLNGDSGSNLQNEPLPEELSIL
                     PLRNMVLFPGVVLPITVGRSKSIRLVKENYKQNRFIGVVAQRNADTEDPDFNDLYTTG
                     TLAQIMKVLQMPDGSTNVIIQGKQRFVITETLLTEPYHTAKVKAFEEEPFDVNDQTSQ
                     AVFASIKDLALQIIQKSNNIPAEAAFALQNIENPNFLINFIASNLSIDLNQKQRLLES
                     NNLLHRAHEVLAFLSHEVQMIDLKNEIQNKVKAELDKQQRDYILQQQMKTIQEELGDD
                     PNKRLIGELKEKATAKNWLPQVAEVFERELSKLQRMHSASAEYSVQVNYLETLVDLPW
                     NDFTVDNLDLGRARRILDEDHFGLQKVKNRLLEYLAVLKLKSDTKSPILCLVGPPGVG
                     KTSLGKSLARALERKYVRMSLGGLRDEAEIRGHRKTYVGAMPGRIIQQLKKARSSNPV
                     FVLDEIDKVSGNNINGDPEAALLEVLDPEQNKAFYDNFLELDFDLSGVMFIATANTMA
                     TVHPALRDRMEIIEIPGYLLEEKIQIAQRHLIPKQLAEHGFKKNQLQFDDKTIALIIE
                     QYTRESGVRLLEKNIAKVIRHHAMLLAENKRIHKKVSPKLVEEILGVPIFHQETAIDN
                     NVAGVVTGLAWTPAGGEVMFIEVSLSRGDGKFSMTGNLGDVMKESATLAFEYLKAHAA
                     DLDIDSNVFKTWNVHIHVPEGATPKDGPSAGISMFTALASAFTQRKVKPRLAMTGEIT
                     LRGKVLPVGGIREKILAAKRARYTDIILPSVNQHDLKEIEPSYIEGLQLHFVDDMKTV
                     LQLALLPEKVEKPLNLSVEK"
     gene            complement(6639..8024)
                     /gene="murD"
                     /locus_tag="PLF20_05535"
     CDS             complement(6639..8024)
                     /gene="murD"
                     /locus_tag="PLF20_05535"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOX80501.1"
                     /translation="MNWEKLKNLLGNKTLCILGFGREGKATLDFLLQHFSGDIVVADM
                     NPETKNYLSSYHSSHLLIQTGEHYLDEVNHCDLIIKTPGIPRSELRGKIDLRKITSQA
                     DLFLSIFSSQVTGVTGTKGKSTTSSLIHHIFSSAGKHSVLLGNIGTPAMSLFDKIRPE
                     TSVVFELSSHQLEDVGHSPHISLLLNIFEEHLDHYSSFEEYRQAKYNITRYQKSDDVL
                     IYHSDNSHLQNSVINIKSKAILMPYSMEYKQGMAAWWEKDIICIIDGNETRRIVPEGP
                     LMLRGEHNRSNILAACTACLLNKIPEKIIGHALPLFEPLPHRLEYVGHIDGKHFYNDS
                     IATIPEATIAAIHTLGKVDTLIVGGYNRGINYEHLIEFLAESEVFHLMLIGEVGKIIE
                     EGLRSTRFNGEIRFYNDFEQLVEDAILITPEGGTCLLSPAAASYDRFINFEERGNLFK
                     NIVLSYSRGMK"
     gene            complement(7987..9387)
                     /locus_tag="PLF20_05540"
     CDS             complement(7987..9387)
                     /locus_tag="PLF20_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80502.1"
                     /translation="MYIQHRNDDFIALRKKFPQFSFDSFAIHHEKDKIDFAYSFSISD
                     RYFFEPKIIIPIKPFFKIKELNPEHLNILAFHVGMIELISYWKASCSPLIKIKPFQLN
                     ADQIKWWKNLYYNGLGEFFYRNNIQVDSEDFVEIQCESQKDISSISFNGNEGYIIPIG
                     GGKDSVVTLELLKKLHLPLVPFIINPREATTETARQAGFRDENVFTVYRTIHPLLLQL
                     NDQGFLNGHTPFSALLAFYTLLASYISGIPRIALSNESSANESTVAGTLINHQYSKSF
                     QFENDFRNYVHKYLDPGLNYFSFLRPLNELQIAGIFSRFPHYHSIFRSCNVGSKTNRW
                     CGHCPKCLFAWIILEPFLGHSYLEKIFGTDLMNEPSLEGTLKELAGYGDMKPFECIGT
                     IEEVKLALSVSITKRPEPLPLLLEIASHFELPSHEILHSFVKNLNPDHFLNPEELSLL
                     KQAIYELGKAEEPARK"
     gene            9484..10074
                     /locus_tag="PLF20_05545"
     CDS             9484..10074
                     /locus_tag="PLF20_05545"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOX80503.1"
                     /translation="MNKLDKILTIRYNEVKPEAGKLLIAEPFLGDAYFGRSVVLLAGH
                     GEEGSFGLILNKQVQVQIHEIVKGMPTFGAKVFLGGPVHSTQIFYLHTIGEKLDGSLE
                     VIPGLWWGGDTDQLWAMIENGEAQSEEVIFYLGYSGWAPKQLEGELKQNSWIVTSAST
                     RFILNTPPSKLWANLLRNMGEHYAYWVNFPQNPQTN"
     gene            10137..10751
                     /locus_tag="PLF20_05550"
     CDS             10137..10751
                     /locus_tag="PLF20_05550"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOX80504.1"
                     /translation="MLLKLYEQNPNPRHIQKIVECLRENGVIIYPTDTVYGMGANIYS
                     PKALERMARIKGIKVEKAEFSIICHDLSQISEYCLPIPNHIFKVMKRLLPGPYTFILK
                     ANSNVPRIFQSRKKTIGIRVPDNEIARAIVRELGNPIANTSIRDEDEILEYTTEPELI
                     YEKYKTLVDIVVDGGYGDNVPSTVLDCTSNEIIIVREGKGPIDF"
     gene            10864..10936
                     /locus_tag="PLF20_05555"
     tRNA            10864..10936
                     /locus_tag="PLF20_05555"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10897..10899,aa:Lys,seq:ttt)
     gene            complement(11106..>11430)
                     /locus_tag="PLF20_05560"
     CDS             complement(11106..>11430)
                     /locus_tag="PLF20_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="disulfide bond formation protein DsbD"
                     /protein_id="HOX80505.1"
                     /translation="EARVWSDPKVLQRLRNDFVIIALYVDDKTPIPENEWVISSYDGK
                     VKKTIGKKYADFQISKFNVNAQPYYVILDTNGQPLIQPKAYDLNIDNFIQFLDAGKQA
                     FYRKD"
BASE COUNT         3274 a         2233 c         2429 g         3494 t
ORIGIN      
        1 ttccaactga gaaatcatgg gttccatttc tatatcaatg cgacttctcc cctggatagg
       61 ttcttctatg actttcatcc ccacaaaact gaataccaga atcccgttgt tgggggcatt
      121 gagcatgtaa ttcccgtcaa tgtcagtaac agttccagtt gtagtgcctt ttaaaagaac
      181 cgttaccccg ggtatggtcg ttccatcctt cttatttttg acctgtccct tgatggtgat
      241 aacctgctgg gccatcatcc caaaactgag gccaaataac aacagcgtaa gcatgtagag
      301 ctttctcata ttcgtttaat ttaagtatta aaggtcgaat cgaatcataa aaataagcaa
      361 ttttcggttt tgaataacat ttcagaataa atttttgttc tttcatgcaa tggaatgaat
      421 gtattcgagt cacatcgagt aagggtcgat ttaaaggatt agtgtaaatt gaacacaaat
      481 acaaaaatag tttataattt gaatttatcg atcatgatgt taaaaattct ttttaattcg
      541 aatgaaatgt ggtgctttag agaattttca tttcgatgaa atgttataaa tgtattgtat
      601 gcaatgtgtt cgtttgattg tttttcgatc gaataaatta aaatgatttg tttggataga
      661 ttgaaaatct ttatatttgt aggctgagtt ttaagcaagt gtattagtta cacaaaacat
      721 cagtcatggc aatatggacc caaatgtaat ccctaatgtt tcggttgatt gtgtggtaat
      781 tggatatgat ctgcaggata tcagtatttt actgatagaa agagaaatgg tttataatgg
      841 gaaaaagtat atcgatttaa aacttccggg tgatctggta agatgggatg aagacattga
      901 tagctcagcc tatcgtattt tatttgattt atcgggtgta caaatcagta atttgaagca
      961 gcttcaggcc tttggtgagg tagatcgctt gaagaaagag ccaagagata tggaatggtt
     1021 acgtgctatc gatcatcccg aagaaagggt aattaccgta gcctattaca ctttggtaag
     1081 tcttgatctt attcattctc aaaaattcaa acctaatcct aatgcgcgct gggtgcctat
     1141 atcgaaaatc ggtaagcttg cgttcgatca taaccgtatc attaataaag ccttagatac
     1201 attgcgtcag gaaataaaac aaactcccgt ggccttcgat ctattgccgc gcaaatttac
     1261 tctttcgcaa atgcaaaaac tctatgaagt agtgatgggt acattattcg acaaacgcaa
     1321 tttcagaaag aaaatagctc agataccctg tatagttcct attaaggaaa aacaagtcaa
     1381 tgtacctcac aaaccagctc gttattatat gtttagccgc gatgtttatg aggtgacgcg
     1441 aaaagacaat tttgatttct cggtataaac gaaacaaagg ccgagcttaa ctttcgagcc
     1501 aacctccttt gaatcctgct ttaatcaatc cctctctaat gtatgaattt ttccttagaa
     1561 tattccaaat caactggtta taataatttt gtatcataat taatataggt ccttggtcga
     1621 ttccaagata atcgatgtcg taccatccaa actcgttatt ttgatcttta taaaatggat
     1681 tgaacgcatc tttgaatcca tacataccat agattttatc tccgtaaaga gtgtacatgg
     1741 ctctgagtgc aggaatgcaa atctccggag caaacgggat ggagccacca gcggcagttg
     1801 gagctatagt tccgtcatct acaagataat ttgcagcggc acctctggcc gagtaggaat
     1861 gaattcttat ctgtctttca tcggattgta tgacggtatc tttagggcca tcgcatgctg
     1921 tgagccccca tatactatca ttataatgag aaaatccaag aggattcgaa atacagtaag
     1981 ccctgttggc cagggttgct cttctcgaat tctcgaaata atctataccc ttgcttagca
     2041 tgaaagagtc ctgtaaacct ttaaaatcta taaataaatg agaatattga tggccaaaca
     2101 gagggccgaa gttcacgtgt tcgtaacctt gaaattttgc ccagatgtag gtactgcacc
     2161 acctttgcca ggcattggca ggaattggat gggtgggaga agcaatagcc aatatcataa
     2221 gtatcatggc ttcgttataa cctgaccacc atgaggggat gaaaccgttt tcgggatgcc
     2281 agcccatact taaggcatac tgggtcgaat cgaacatcca atcccactcc actcttctat
     2341 aaagactatc ggccaattgc ctgatttcgg cttctactga attgtcccta tcaaaatagc
     2401 tcatacatgt aaggattcca gccattaata aacctgtatc gatggatgag agttcaacct
     2461 ttttgaacct cgaacctgtt ttcatgtcga gaaaatgata ataaaacccc ttataacctg
     2521 aaattttgga tgtttgagcc ccttggggaa gattatagag aaatttcaat gagtttttta
     2581 ctttgagggc tacgtcttca cgcgttaccc agccattttc tatcccaata atccatgccg
     2641 gataaccaaa tccctgggct gctatgctcg aaaaggatgg ggtgggccaa cgatcgggta
     2701 caagattggt taccggatcg gcacttaccc aaaaatacct gaaggttctt ttttgaaggt
     2761 cctcgagtga gaattcttgt ttcgagattc tattcgtaca cgagaaaatc agcagaataa
     2821 tcataaaaag accgataggt tttttcatat acttatgctt aggaagtgca aaataataac
     2881 aactgcattc gtaaagcaat gttttatggt agaactatta aattttgtta ggtagatatg
     2941 ttctaatttt gtaggtttaa tgataaaaac agattaatga tgagcgatga gtatttatat
     3001 attggggatt gaatcttcgt gtgatgatac ttcggctgct gtgctccgtg acgatttatt
     3061 gctaagtaat gtaattgcca atcaggagat ccattacaag tttggtggtg tggtacccga
     3121 attggcttcg agggcacatc agcagagcat tattcctgtt gtggatcagg ctatttggca
     3181 ggctaaactt actccacagc aaattcagct tgtagcattt acgcaagggc caggtttgtt
     3241 gggttcactt ctggtggggg tttcttttgc caaagccttt gcgttggccc ttggtattcc
     3301 tatgacgggt tgcgaccaca tgcaggcaca tatcctatct catttcatcc ggaaacctgg
     3361 ctgcaaacat cgagtcccat cttttccctt tctatgtctt accgtttcgg gaggtcatac
     3421 gcaaatcgta aaagtcgacg attacgataa gatgaaaata atgggacgta cgatcgacga
     3481 tgccgcaggt gaaactttcg ataaggccgc taaaatcatg ggattgcctt atccgggggg
     3541 gcctgtaatc gatcgacatg ctcgtaaggg aaatcctatg gctttttctt ttgctaaacc
     3601 cctgattgct ggtctcgatt ttagctttag cggcttgaaa acttccttcc tgtatttttt
     3661 acgcgaccgt ctgaaagaaa atccttcgtt tatttcggaa tatctcgatg atttatgcgc
     3721 ttccattcaa aaatccatcg tcgatatctt aatggaaaag ttgaagaagg cagtggagat
     3781 caccggtata accgaagtag ccattgctgg tggagtttcg gccaattctg gactccgtga
     3841 ggcattaatt cgtgcaggtc aggaaggggc atggaatgtc tatatccctg aatttgcatt
     3901 tactaccgat aatgctgcta tgattgctat ttcgggttat ttgaaatatc tgaaaggaga
     3961 ttattctatg caagatgcta ttccttatgc ccgaagccgg atgcaataat aacacccttc
     4021 gatgtgattt ttcttcaacg aaaatgagaa ttttattttt ccactgataa attcaggggt
     4081 ttttcgactt tctcgggcaa taaagccagt tgaagcacgg ttttcatatc atccacaaag
     4141 tgaagttgaa gcccttcgat ataggaaggt tcgatttcct tcaggtcatg ttggttgact
     4201 gaaggaagga taatatcggt gtatctggct ctttttgctg ccagaatttt ttctcttata
     4261 ccccctaccg ggagtacttt tcccctcaat gtaatttctc cggtcatagc aagccgaggt
     4321 ttaacttttc gttgtgtaaa tgcggatgcc agagcagtaa acatgctaat tcctgccgaa
     4381 ggaccgtctt tgggtgtagc accttcggga acgtgtatat gcacattcca agttttgaaa
     4441 acattgctgt cgatatccag atcagctgca tgagccttta aatactcaaa tgctaaagtg
     4501 gccgattcct tcattacgtc tccgagattt cccgtcatcg aaaatttccc atcaccacgg
     4561 cttaggctca cttcgataaa cataacctcg cctcctgcag gagtccaagc taatccggta
     4621 actaccccag ctacattgtt gtcgatggct gtttcttgat gaaaaatggg aactccgaga
     4681 atttcttcta ctaatttggg actcactttt ttgtgaattc ttttgttttc ggccagcagc
     4741 atggcatgat gacgtattac tttggcaatg ttcttttcga gcaatcttac tccactttcg
     4801 cgggtgtatt gttcgataat taacgctatg gttttatcat cgaactggag ctggtttttt
     4861 ttgaatccat gttcggccag ctgtttagga atgagatgcc tctgggctat ctgaatcttt
     4921 tcttcgagca aatatccagg tatctcgatg atttccatgc ggtcacgcaa tgcaggatga
     4981 acggtagcca tagtattggc tgtagctatg aacattactc ccgaaagatc gaaatcgagt
     5041 tcgagaaaat tatcgtagaa tgctttgttc tgttcagggt cgagtacctc gagcaaagca
     5101 gcttcgggat caccattgat gttgtttcca ctcactttgt caatttcatc gagcacaaaa
     5161 accggattag atgaccgggc tttctttagc tgttgaataa ttctaccggg catagcaccg
     5221 acataagttt tccgatgccc gcgtatttca gcctcatcgc gtagtcctcc cagtgacatg
     5281 cggacatact ttctttccag tgcgcgggca agcgattttc ctaaggaagt tttccctaca
     5341 cccggtggcc ctaccaaaca aagtatggga gatttggtat cgcttttcaa ttttagaacg
     5401 gctaaatatt ctaatagacg attctttact ttttgcagcc caaagtggtc ttcgtcgaga
     5461 attcgtcgtg ccctgccgag gtcgagattg tcaaccgtaa aatcattcca tggtaagtct
     5521 accagagttt ccagataatt cacctgtacg gaatattctg ccgaagcact atgcatacgc
     5581 tgcaacttac tcaattcacg ctcgaagact tcggccacct gagggagcca attttttgca
     5641 gtagcttttt cctttaattc cccgatcaac cttttgttgg ggtcgtctcc aagctcttcc
     5701 tgtatggttt tcatctgctg ctgcaatatg taatcgcgct gctgcttgtc gagttcggct
     5761 tttaccttgt tctgaatttc attctttaag tcgatcatct gcacttcgtg gctaaggaaa
     5821 gctaaaactt cgtgagctcg atgcagaaga ttattcgatt ctaataagcg ttgtttctga
     5881 tttaaatcga tggatagatt ggaagcaatg aaattgataa gaaaattggg attttcaata
     5941 ttttgaagtg caaatgcagc ttctgcagga atattgttcg atttctgaat tatttgcaag
     6001 gctaagtctt taatagatgc aaacactgcc tggctggttt ggtcgttcac atcgaagggt
     6061 tcttcttcga aagctttaac cttggccgta tgatagggtt ctgtgagtag ggtttcagta
     6121 attacgaaac gttgtttccc ctgaatgatg acattggtcg acccgtcggg catttgcagg
     6181 actttcatga tttgtgctag tgtgcctgta gtatacagat cattgaagtc gggatcttcg
     6241 gtatcggcat ttcgttgggc tacgacccca ataaacctgt tttgcttata gttttctttt
     6301 acaagccgaa tggatttact acgtcctacg gtaatgggca gaacaacgcc aggaaacaga
     6361 accatgttac gaagcggaag tatggataat tcttcgggca atggttcgtt ttgaagattc
     6421 gaccctgaat cgccatttaa taatggaata aactctacat tcccctctat taaatcagaa
     6481 agtaagtaag gtgtatcata gattttcata gaattgccat tttgtcagat aaaaacctgc
     6541 cccctcccgc aaattccggt agagtggctg gtatgcgttc tttagagtga tagactggta
     6601 gtcggctgga taatttcaat aaatatgcca taaatatttt atttcatccc tctagaataa
     6661 gatagaacaa tatttttgaa aagattccct ctttcttcga aatttatgaa tctatcgtaa
     6721 cttgctgctg ccggtgaaag aagacaagtg ccgccttcgg gtgtaataag aatagcgtct
     6781 tcgacaagtt gctcgaaatc attgtaaaat cttatttctc cgttaaaacg ggttgatctt
     6841 aagccttcct cgataatttt tcctacttct cctatgagca ttaaatggaa aacttcggat
     6901 tcagccagaa attcgatgag gtgttcgtag ttgattcctc ggttgtaacc accgacgatc
     6961 aacgtatcta cttttccaag ggtgtgaata gcggcaatgg tagcctctgg aatagttgca
     7021 atagaatcgt tgtaaaaatg ttttccgtcg atgtggccta catattccaa tctgtggggc
     7081 agaggttcga aaagaggtaa agcatgcccg ataatttttt cgggaatctt atttaacagg
     7141 caggccgtgc acgcagccag aatattcgag cggttatgtt cgccgcgtaa catcagtggc
     7201 ccttccggaa cgattcttct tgtttcgttt ccatcaatta tacagatgat atctttttcc
     7261 caccaggctg ccattccctg tttgtattcc atggaataag gcataagaat ggctttcgat
     7321 tttatattaa tgaccgaatt ctgcaaatga ctattatctg aatggtatat aagaacatca
     7381 tcagactttt gataacgagt gatattgtat ttggcttgac gatactcttc gaaagacgaa
     7441 taatggtcga ggtgctcctc gaaaatattc aagagaaggc ttatatgtgg tgagtggcct
     7501 acgtcttcga gctgatggga tgaaagttcg aaaacgactg aggtctcggg tcttatcttg
     7561 tcaaacaggc tcatggccgg cgtaccaata ttccccaata aaacgctatg tttgcccgcc
     7621 gatgaaaata tgtgatgaat caaactgctg gtggtgcttt tgcctttggt gccagtaaca
     7681 cccgtaacct gagatgagaa gatactgaga aacaaatcag cctgagaagt tatttttcgc
     7741 aagtcgattt ttcctctgag ttccgatctc ggtatgcctg gagttttgat aatcagatca
     7801 caatgattca cctcatccag ataatgttct ccggtttgaa ttaataaatg agaagaatga
     7861 taggaagata gataattctt tgtttcggga ttcatatcgg ccactacgat atcgcccgaa
     7921 aaatgttgca gtaaaaaatc gagtgtagcc ttaccctcac gaccaaaacc caaaatgcac
     7981 agggttttat ttccgagcag gttcttcagc ttttcccaat tcataaattg cttgctttaa
     8041 aaggcttaat tcctctggat tgaggaaatg atcaggattc aaatttttta caaagctatg
     8101 gagaatttcg tgtgaaggca gctcgaaatg tgaggcaatc tcaagcagaa ggggtaaagg
     8161 ttcaggcctt tttgtaatac tcaccgaaag ggctaatttg acttcttcta tcgttccgat
     8221 gcattcgaaa ggtttcatat cgccatagcc tgccagttct ttgagtgttc cctccagcga
     8281 aggctcattc atcagatcgg ttccaaagat tttttcgaga tatgaatgac ctaaaaaagg
     8341 ctccagtata atccatgcga agagacattt agggcaatgg ccacaccatc tattggtttt
     8401 gctacctaca ttgcagcttc tgaaaatgga atgataatgt ggaaatcgcg aaaatatacc
     8461 tgcaatctgc agttcattca atggtcggag aaaactgaag taattcagtc caggatcaag
     8521 gtatttatga acgtagtttc tgaaatcatt ttcgaactga aacgatttgg aatattggtg
     8581 attgataagt gttccagcaa ccgtgctctc gttggcgctc gattcgttcg aaagcgctat
     8641 ccggggaatt ccactgatat atgatgccag caatgtataa aaagccaata aagccgaaaa
     8701 aggggtatgc ccattcagaa agccctggtc gttgagttga aggagcaaag gatgtatggt
     8761 acgatagacg gtaaaaacat tttcatcgcg gaatcctgcc tgacgtgcag tttcggttgt
     8821 ggcctcgcga ggattgatga taaaggggac aaggggtaga tgcagttttt tcaatagttc
     8881 gagcgttacg accgaatctt taccgcctcc tatagggatg atataaccct cattgccatt
     8941 gaaactgatg gaagaaatat ctttctgact ttcacattgt atttctacaa aatcctctga
     9001 atcaacctgt atattattgc gatagaaaaa ttctcccaat ccattgtaat aaagattctt
     9061 ccaccattta atctgatcgg catttaattg aaaaggcttt atctttatca aaggagaaca
     9121 gcttgctttc caataactta tcagttcgat cattccgaca tgaaaggcaa gaatattgag
     9181 atgttcggga ttcaattcct tgattttgaa aaatggcttg atggggatta taattttggg
     9241 ttcgaaaaaa taccgatcgc taatgctgaa agaatatgca aagtcgattt tatccttttc
     9301 atgatgaatg gcgaatgaat cgaacgaaaa ctgggggaat tttttacgaa gggctatgaa
     9361 atcatcattg cgatgttgaa tatacataaa ttttggtata ttttttgaaa atttaacagc
     9421 gaatcaggca acctttttta aaatgagcgt ccaaagatat aaagaaaagc ccatcacact
     9481 aagatgaaca agttggataa gatactgact ataaggtaca atgaggtgaa gcccgaggca
     9541 gggaagctat tgatagccga acctttcctg ggggatgcct attttggcag gtcggtagtc
     9601 ttattggccg gacatggcga agaaggttcg tttggattga ttttgaacaa gcaggttcag
     9661 gttcaaattc atgaaattgt gaaagggatg cctacgtttg gagctaaagt ttttttaggt
     9721 ggccctgtcc actccacaca gatattttat cttcatacca tcggagagaa actggatggg
     9781 agcctggaag tcattccggg actgtggtgg ggaggtgata ccgaccaact ctgggctatg
     9841 atagaaaatg gagaagcaca aagcgaagag gttattttct atctgggata cagtggatgg
     9901 gctccaaaac aactcgaagg agaactgaag cagaattcat ggatagtgac ctctgcttcc
     9961 actcgtttta tcttgaatac tccaccctcg aaactctggg caaacctgct aagaaacatg
    10021 ggagaacatt atgcttactg ggtgaatttt ccccaaaatc cccagacaaa ttaatttacc
    10081 tgtttcttga aatcgataat ttttgcattt tattttaaaa ccaaaatgtc atgagtatgc
    10141 tgttgaaatt atatgaacaa aatcctaatc ctcgccacat tcagaagatt gtcgaatgtt
    10201 tgcgagagaa cggggtgatt atttatccaa ccgatacggt ttatgggatg ggggccaata
    10261 tttacagccc gaaagcactg gaaagaatgg ccagaatcaa gggaataaag gttgaaaagg
    10321 ccgagttttc tattatttgc catgacttaa gtcaaatatc agagtattgt cttcctattc
    10381 ccaatcatat tttcaaggta atgaagcgtc ttttgccggg gccttacacc tttattctca
    10441 aagccaatag caatgttccg cggatcttcc agagtcgtaa aaaaaccata ggaataagag
    10501 ttcctgacaa tgagattgct cgtgccattg tgcgtgaatt aggcaacccg atagccaaca
    10561 cctcgatccg cgatgaggac gaaattttgg aatacaccac cgaacccgag ttgatttatg
    10621 aaaaatataa aacattggtg gatattgtgg tagatggcgg ctatggcgac aatgtacctt
    10681 cgacggtgct ggattgtacg agcaatgaaa tcataatcgt gagagaagga aagggtccta
    10741 ttgacttttg atcttttccg tcaatccttt ttgaatttta aataaaacaa tccacaaatt
    10801 ttgataactt aataaaaata gctacttttg ccgcccgaaa acgaaagaga aaacagtgct
    10861 cacgactacg tagctcagca ggcagagcac atccctttta aggatggggt cctgggttcg
    10921 aatcccagcg tggtcacttg aattctccgg tcgaaaatcg gagtttttta atatctaccg
    10981 cagaaaatcg acactaaatc tatagataat cggagagagc ccaacgagct ttctgttcga
    11041 gaataataga aaaactctga ttatcgaaaa aataatcaga gtttgataat aaaactgaca
    11101 ataggttagt cttttcgata gaaggcctgc ttgcctgcat cgagaaattg aatgaaattg
    11161 tcgatattta gatcgtaagc cttaggctgt atcagaggtt gaccatttgt gtcgagaata
    11221 acataataag gctgagcgtt tacattgaat ttcgaaattt gaaaatcggc gtatttttta
    11281 ccaatcgttt ttttcacctt gccgtcatat gacgagataa cccattcatt ttcaggtata
    11341 ggtgttttgt catccacata taaagcaata atcacgaagt cgtttcttaa tctttgcaag
    11401 acttttggat cggaccaaac cctggcctcc
//
LOCUS       DAOORU010000066        11361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7132_length_11361_cov_6.247479, whole genome shotgun sequence.
ACCESSION   DAOORU010000066 DAOORU010000000
VERSION     DAOORU010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11361
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7132_length_11361_cov_6.247479"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /locus_tag="PLF20_05565"
     CDS             complement(<1..1839)
                     /locus_tag="PLF20_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOX80506.1"
                     /translation="MNRKFLLSLLGIFFFLTQLPQDYAQVLDPVKWSFSSQKINSDEY
                     ELIFTAKIDKNWHLYSQYVPEGGPVPTNFIFEPSSNFKLIGKTTEPRPHKMYDQNFEM
                     EVQYFDGQAIFKQRIKSLTQQSFNVRGTVEFMACDDSRCIPPAEVDFDFAISPSAEPD
                     KAVTGVQKTETAYQLNTDSSFGEISEENIATSANDSVALNTSLGGEQKERHSNLWAFF
                     CLAFLGGLIAIFTPCVFPMIPMTVSFFMHDEENKKQARRQALIYGLSIIVIYTVIGTL
                     VALTLGPNFANFLSTHWLPNLFFFVIFMVFAASFFGAFDLTLPHWMANKADQNAEKGG
                     ILGPFFMAFTLVLVSFSCTAPIVGTILVASASGEILKPIIGMAGFGLAFAFPFTLFAL
                     FPSWLGTLPKSGGWLNSVKVVLGFIEVAFGLKFLSVADQTYHWGILDREIYIAIWIVV
                     FTLMGFYLLGKIRFKHDDEVHHIGVFRLTLVIATFSFVVYLLPGMWGAPLKALSGYLP
                     PQSSHDFDLNQIIRENVSANLNILPQQSNPSVPTEQCENPKYADIFHLPHGLKGYFDY
                     QQGLACAKQLNKPIFIDFTGHGCVNCREMEARVWSDPKVLQRLRNDF"
     gene            complement(1882..2754)
                     /locus_tag="PLF20_05570"
     CDS             complement(1882..2754)
                     /locus_tag="PLF20_05570"
                     /inference="COORDINATES: protein motif:HMM:NF033782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28 family outer membrane lipoprotein"
                     /protein_id="HOX80507.1"
                     /translation="MKKHHPTSVIIITWIFTMMQIACDKVDAPYLEHIQTYLDTAQTS
                     DTSYKRIALLEDFTGHKCVNCPQAAQLAKNLQLAHKGQIVVVEIHAGFFAVPDQSGLY
                     IADYRCTEGTSLNDYFNIMQYPTGLVNRKAYEGKIRLLPTSWESAILAELKQEPLAFI
                     QLENTFNPQNRNLRIETDIKILQNLEGEYTYTLYLIENHLISSQKNNNSFVGPVPDIT
                     DYVHNHVLRTSINGIWGETLHPGNGFVKDETIQLIHNFIMSENWNASQCHVVALMSKV
                     GTNEVIQAAEESIE"
     gene            complement(2847..4571)
                     /locus_tag="PLF20_05575"
     CDS             complement(2847..4571)
                     /locus_tag="PLF20_05575"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HOX80508.1"
                     /translation="MKKLLRITLPFVLLSCSLYVKSQDFIHNAQVSGTFQADANAYVN
                     DKLLHINDSTLNGKRLGINGFALIHYDYKNFSAGLRYEAFLPPLLGFSPDAEGSGIAN
                     WFVNYKVDNFEVTAGNFYEQFGSGMILRSYEEWTLGYDNALDGFRVKFKPYHGIYLKG
                     LMGYQRNYWVKYSEGNRGLVRGIDGELNFNELIEKLGNSPFRLSLEASFVSKYEKMPL
                     KTIIRPILPEGSYEVYQYKLPENVGVWLAAINLGYSNWNLMTEYVRKSENPSALNNYI
                     YREGQALLSVLTYSKKGLGIELSAKRIDNMGFKSRMEEKENVLDINFLPPLNPLHLYS
                     FATMYPYATQPNGEMAVHTGIEYTFPKNTLLGGKYGTNLSLSYSLIKDIEKQPVSADI
                     PIDSTGTLGYTSDFFAFGDRKFYEDLKIQLTKKFSQRLKMIAAYFNQVYDKDVIEGHT
                     NEYGKVYANIGVVDMTYRINNQHSIRLELQTLFTEQDKGNWMGTTIEYTLAPKWFFSL
                     MDEYNYGNDNPDDRIHYYNISFGYTHQSTRISLSYGRQREGLLCVGGICRYVPAYTGF
                     GLTLSSNF"
     gene            complement(4658..5185)
                     /locus_tag="PLF20_05580"
     CDS             complement(4658..5185)
                     /locus_tag="PLF20_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOX80509.1"
                     /translation="MKNIVLIALFSLLFLNMEGQDPKQSSKGRSLPSVELKTLDRKTL
                     LTDTLSNHGKPMIISFWALWCKPCIKELTNIGNVYKEWVEETGVRLIAVSIDDARSSS
                     RVKPFVDGKGWEFEVLLDPNSDFKRAMNVNLIPHTFLLDGNGKVVWQHTSYTEGSELE
                     LIEMVRKLNQGETIQ"
     gene            complement(5216..5479)
                     /locus_tag="PLF20_05585"
     CDS             complement(5216..5479)
                     /locus_tag="PLF20_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80510.1"
                     /translation="MWGRKLVFLFWNFPFFKQGWHNVLPAKNNLFKPLLVNKMEDMTG
                     KTNRILVEILHKNERLCFVQSFTGQSSIETPPNRFSWKVISNI"
     gene            complement(5649..7871)
                     /locus_tag="PLF20_05590"
     CDS             complement(5649..7871)
                     /locus_tag="PLF20_05590"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX80511.1"
                     /translation="MKCRTFACLFLSLLPIFLYAETEIKLPDTLEKRLGKVRHDTVRV
                     NILNEAAHVYLMNNPDLGRRAAQLALEIASKNNYTLGQHEAMLLLGDYYFYTGNYTEA
                     RNCYLQCLDFSMGLNSLKIAKIYSRLSNISLMGKNLDTAIYYAKINKTLNKKYGSWFD
                     YIRSMQNLGLLYFQNRMYDSAIQEFAKAISISDEMIKNQEPQSPEYYRLLEQNADLHR
                     LLALGWQNKGRFKLALQEMQRGITLAIMCNNTQLQASLYSDMSQILQKQGIYEKALEY
                     LIKAVKIYEKLGEKLMIANAYQTIGKIYIENSDYINAYRFFKNSLQVHKSLDNKAGLA
                     TIYNDFGNLKRIFNETDSAEFYYKKSLEINKLLSNKQEMGINYLNLGILSEERGKHSH
                     ARIYLEEAKKIFEQIDDEENLARVLLEQGLLEANQNHTSLAIDLLLKAEKMASNYGMI
                     PIRKSAAKYLSDIYERTGDYFKAFQYQKLYSILSDTLNNIERQKQILSIQATYDFEKQ
                     QNELLLEQEKRKTLEKNQTLQRVISISSLLALILTGLITYLIFKRIKEKQKIEAEKLL
                     KEAEISKAKETLIETQLRNQDLEKQRLIEELKYKSNHLTNLALIIAQKNEFINELKNL
                     LKELKNSNESQKDKVMKELWQKTTQQGNINKELDKFKKEIDDANRSFYEKLNNICPQL
                     TLHEKELAGLLRINLSSKEIASLHNVSVKAIEMSRYRLRKKLQLESNDKLVDFLQKLD
                     "
     gene            complement(7884..9311)
                     /locus_tag="PLF20_05595"
     CDS             complement(7884..9311)
                     /locus_tag="PLF20_05595"
                     /inference="COORDINATES: protein motif:HMM:NF021021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HOX80512.1"
                     /translation="MSNHRFTVFLFLFCSIFSQNVKSEDIRFSDYFQNKTMRVDYFHT
                     GNNVTEVFAIDRIVNDGDWAGSLTQLQDKMNLGLYFFEVIDKGKNQVIYSRGFASIFG
                     EWQLTPEASEYGSFHESIRFPWPLEPFTLVISKRGSDNFFHKIWEKEIDPNSRFVNRA
                     EPELPFKSWTVIENGDPHYKVDIVVLGDGYRYDQMEKFRADVNRLVSELFAVEPFQSR
                     KSDFNVRAVFTPCQQAGINKPHPGVYARTPLSLTYGAFDSERYVLGFDNRSIREVASL
                     VPYEFMIILVNERTYGGGGIYNLYSTVSADNKFADYIMVHEFGHQFAALTDEYYSSSV
                     SYEGPSIEIEPWEPNITALKDPNNLKWKHLVDPSTPIPTPWNKETFDKFSYQIQIERH
                     EIRARNEPEEVMEALFERERKFETQLINNMEYIGKVGAFEGAGYNQYGMYRPETDCIM
                     FTRNRQAFCKVCQEAISKIINLYVQ"
     gene            9496..10479
                     /locus_tag="PLF20_05600"
     CDS             9496..10479
                     /locus_tag="PLF20_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HOX80513.1"
                     /translation="MDIKLEEFSLSKNESQKGLLQKVGIVGCGIMGQQIALQTSQYGI
                     DVIGLDVDQTRIEEITRCLDDELNRIINHWGMTAAEKKLILSRITLTTHYEDLNDCDI
                     AIETIKSKKPGTSLSMRQEVFHKLEEVLRHDAVIASNTATLMISDLSSVLKDPSRAVG
                     MHFISPVREVKIVETVRSMRTSKDTWDLACKYIRMIGKKVIPVNESPGNISTRMIIPF
                     INEACSILMEGVAMVDQIDETMREASGHMMGPFEMADTIGLDKIMRWMENLYNEFGEI
                     RYKPSPLLKRMVRAGIVGQRVGEGFYKWTPKGKQIKEGPVFTLGRESFFNT"
     gene            10492..>11361
                     /locus_tag="PLF20_05605"
     CDS             10492..>11361
                     /locus_tag="PLF20_05605"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HOX80514.1"
                     /translation="MKIIVLNCGSSSIKYQLFNMPSAEVHAKGLVDKIGLKGASIKHQ
                     RNDGLEFKVEGEILDHQKGIEYVLGILIHPEYGSLKGIHEVDAVGHRVVHAGEKFGGS
                     VRINQDVVGALEECIDLAPLHNPPNLEGIYAITRLLPQIPQVGVFDTAFHQTMPDYVY
                     LYGLPYSLYEKYKIRRYGFHGSSHKYVSLRAAKILGQDYDKLRLITCHLGNGASVAAI
                     KNGKSFDTSMGMTPIEGLIMGTRTGDVDAGALLHIAEKEGIGFQTLNKLINKFSGLLG
                     ISGISSDMRDLEEA"
BASE COUNT         3238 a         2156 c         2428 g         3539 t
ORIGIN      
        1 gaagtcgttt cttaatcttt gcaagacttt tggatcggac caaaccctgg cctccatttc
       61 ccggcaattt acgcagccat gtcccgtaaa atcgataaag atgggtttgt tcaattgttt
      121 tgcacaagct agtccttgtt gataatcgaa atatcctttc aatccatgcg gtaaatggaa
      181 aatatcggca tacttgggat tttcgcattg ttcagtgggg actgaaggat tactttgttg
      241 gggtaatata ttcaggttgg cgcttacatt ttctcgaata atctggttaa ggtcgaagtc
      301 gtgtgatgac tgaggcggca aatagcccga aagggctttg agtggtgcac cccacatgcc
      361 gggcaataga tagactacga acgagaaagt tgcaattaca agcgttaagc gaaatactcc
      421 aatatgatgg acttcgtcgt catgtttgaa acggatttta cccaagagat agaaacccat
      481 taatgtgaat actacgatcc agatggcgat atagatttcg cggtcgagga taccccagtg
      541 ataagtttga tcggcaacgc tcaggaattt caacccaaac gccacttcga taaaacccaa
      601 gacaactttc accgaattca accatccgcc agattttggt aaagtaccca gccaggaggg
      661 gaataaagca aacaaggtaa aagggaaagc aaaggctaaa ccaaagcctg ccataccgat
      721 gatgggcttg aggatttcac cgcttgcaga agctacaagg atggtcccta caataggagc
      781 tgtgcatgaa aaggaaacca atacaagggt gaaagccatg aaaaatggac ccaaaatccc
      841 tcctttttcc gcattttgat cggctttatt tgccatccaa tggggaagag tgagatcgaa
      901 agctccaaaa aaggaagcgg caaataccat aaagatgacg aaaaagaaca gattgggtaa
      961 ccaatgagta ctcagaaagt ttgcaaaatt cggtcctaag gtcaaagcca ctaaagtgcc
     1021 tataactgta taaataacta taatgctcag accataaata agcgcctgcc gtctagcttg
     1081 cttcttgttt tcttcgtcat gcatgaaaaa gctaacagtc atagggatca ttggaaaaac
     1141 gcaaggagtg aaaatggcaa tcaatcctcc aagaaaagct aaacagaaaa aggcccataa
     1201 gttcgaatgc ctttccttct gttcgcctcc aaggctggta ttcaatgcga cactatcgtt
     1261 agcactggtt gcaatgtttt cttctgatat ctccccaaaa gacgaatcgg tatttaactg
     1321 atatgccgtt tcagttttct gaacgccggt aactgctttg tccggttcag cagaaggaga
     1381 aatggcaaaa tcgaaatcca cttcggcagg aggaatgcaa cgagaatcat cgcatgccat
     1441 gaactccacc gttcctctaa cattaaagct ctgctgagtg agtgatttga ttctttgttt
     1501 gaatatggct tgcccgtcga aatactgtac ttccatttcg aaattttggt cgtacatctt
     1561 atggggtctt ggctcggttg tttttcctat taatttaaaa ttggaagaag gttcgaagat
     1621 aaaattggta ggaacaggcc ctccttctgg aacatattga gaatagagat gccagttctt
     1681 gtctatttta gcagtaaaaa ttaactcata ttcatcggaa tttatttttt gggacgaaaa
     1741 actccacttt actggatcga gcacctgggc gtaatcttga ggaagctgcg tcaaaaagaa
     1801 aaaaattcca agcaaggata aaagaaattt tctattcata gattgctatt attcaatttg
     1861 ttaatattct cgaggatttt gctattcaat gctttcttca gcagcctgaa tcacttcatt
     1921 ggttcccacc ttgctcataa gggcaaccac gtggcattga ctggcattcc aattctcact
     1981 cataataaaa ttgtgaatca attgtatggt ttcatcttta acgaatccat tcccaggatg
     2041 taaggtttcg ccccaaattc cattaatgga agtccgaagt acatgattgt ggacataatc
     2101 ggttatatcg ggcacaggac caacgaaaga attgttgttt ttttgagacg aaatgagatg
     2161 gttttcgatt agataaagcg tataggtata ttctccttcg aggttttgta aaattttaat
     2221 gtcagtttca attcgaagat tgcgattttg agggttaaat gtgttttcga gttggatgaa
     2281 ggccaatggt tcttgcttaa gctccgccag tatggccgat tcccagcttg tgggaagcaa
     2341 tcttatcttt ccttcgtaag cttttcggtt taccaggccg gtaggatatt gcatgatgtt
     2401 gaaataatcg ttcaatgaag ttccttccgt acagcgatag tcagcaatat agagccctga
     2461 ttgatcggga actgcaaaaa aacctgcatg aatttccacc acgacaatct gacctttatg
     2521 agccaactgt aaattttttg ccagttgtgc ggcttggggg cagttcacac atttgtgacc
     2581 ggtaaaatct tctaatagcg cgattctctt ataggaagta tccgatgttt gtgccgtatc
     2641 aagatatgtt tgaatatgtt cgaggtaggg agcatccact ttatcgcagg caatttgcat
     2701 catcgtaaaa atccatgtaa taattatgac tgaagtcggg tggtgttttt tcatattgtt
     2761 ctataagttg ttattatgtt gattgaaaat attcgttttg aaaaaatttt ctagatattt
     2821 cgattcttca aacatttcaa aacgatttaa aaattgctcg aaagtgtcag tccgaaaccg
     2881 gtataagcgg gtacatatcg gcaaatacct cctacacaca gtaaaccttc tctttgtcgg
     2941 ccataggaaa gagaaatgcg ggtagattga tgtgtatatc caaacgatat attataataa
     3001 tgtatccggt catcgggatt atcgtttcca tagttatatt catccatgag ggaaaagaac
     3061 cattttggag cgagtgtgta ttcaatcgtg gttcccatcc aatttccctt atcctgctca
     3121 gtaaatagag tttgcaattc aagccgtatg gaatgctgat tgttgatacg ataagtcata
     3181 tccacgactc caatgtttgc atatactttt ccatattcgt tcgtgtgtcc ttcgattaca
     3241 tctttgtcgt agacttgatt gaaataggct gcaatcattt ttaaccgctg tgaaaacttt
     3301 tttgtaagct gtatcttcaa gtcttcgtag aatttgcggt ctccgaaggc gaaaaaatca
     3361 gaggtatagc ccagggtgcc ggtgctgtcg atgggaatat cagctgaaac aggttgtttt
     3421 tcgatatctt tgatcaatga ataggaaagg ctgagattag tgccatattt gcctccaagt
     3481 aaggtattct tggggaaagt atattcgatt ccggtgtgaa cggccatttc gccattgggt
     3541 tgagttgcat aaggatacat tgtagcgaag ctataaaggt gcaagggatt aagaggtggc
     3601 aggaagttga tatccaaaac attctccttt tcttccattc tcgatttaaa gcccatgtta
     3661 tcgattcgtt tggccgagag ttcaattccc aggccttttt tcgagtaagt aagtacgctc
     3721 aatagagcct ggccttcgcg atagatataa ttattcaagg cggatggatt ttcgcttttg
     3781 cgaacatatt ccgtcatcag attccagtta gaatagccaa gattaatggc tgccaaccaa
     3841 actcccacat tttcgggcaa tttgtattga tatacctcat agctgccttc gggaagaatg
     3901 ggccgtatta tggtttttag gggcattttt tcgtatttac ttacgaagct tgcttccaaa
     3961 ctcaatcgaa aaggactatt tcccagtttt tcgatcagtt cgttgaaatt caactcccca
     4021 tcgatgcctc ttaccaaccc tcgatttcct tcggaatatt ttacccagta attgcgctga
     4081 tagcccatca agcctttcag ataaattcca tgataaggtt tgaattttac tctgaatcca
     4141 tcgagtgcat tatcgtagcc aagtgtccat tcttcatacg aacgaaggat catacccgaa
     4201 ccgaactgtt catagaaatt tccagcagtt acttcaaaat tatcgacctt gtagttgaca
     4261 aaccagttgg ctattccact tccttcggca tcgggcgaaa atccgagcaa gggaggcaaa
     4321 aaggcttcat accgcaagcc tgccgaaaaa ttcttgtaat cataatgaat aagagcaaaa
     4381 ccatttattc ccaatctctt gccatttaac gtactgtcgt tgatgtggag taatttatca
     4441 ttgacatagg cattggcatc ggcctgaaaa gttccactta cctgtgcatt gtgaataaaa
     4501 tcctgactct ttacatatag agaacatgac aatagaacga agggcaaagt gattctcagt
     4561 aattttttca tatcgataat aaaaagaaat aaatttttat tacttagatg attcctcgat
     4621 ttttcgaaca ttctggtcaa tattggatta ttctaattta ttggatggtt tcgccctgat
     4681 tgagttttct gaccatttcg atgagttcga gttcgcttcc ttcagtgtag gaggtatgct
     4741 gccagactac tttgccgttg ccatcgagca gaaaggtatg aggaatcagg tttacattca
     4801 tggcgcgctt gaagtcgctg ttgggatcga gcaaaacttc gaattcccag cctttcccat
     4861 cgacaaaagg ttttacgcgg gacgaagaac gcgcatcatc gatagaaacg gcgatgagtc
     4921 ttactccggt ttcttcgacc cactctttat aaacgttgcc aatgttggta agctccttga
     4981 tgcaaggttt acaccaaagc gcccaaaaac tgatgatcat gggtttgcca tggttgctca
     5041 aagtatccgt aagtagggtt tttctgtcta aggttttcaa ttcaaccgat ggcaaggaac
     5101 gacctttcga agattgcttt gggtcctgtc cctccatatt taaaaacaat aaggaaaata
     5161 atgcaatcag aacgatgttt ttcatggcgt taaagtattg aatgagtata attaatcaga
     5221 tgttcgaaat tactttccat gagaatctgt tgggtggagt ttcgatggaa gattgaccgg
     5281 taaacgattg aacaaagcat aatctttcgt tcttatgcaa aatttcgacc aaaatcctgt
     5341 tcgttttgcc ggtcatgtct tccatcttat ttactaaaag tggtttgaac aagttgtttt
     5401 tggcaggaag cacattgtgc caaccttgtt tgaagaacgg gaaattccag aacaaaaata
     5461 caagtttcct accccacata cgtattttta tattctatca tacttctaca agtcttggat
     5521 tttggcatat tattgagtga atttacctct acaattaatt taactaaatt attaaaaatc
     5581 aattaatatt catataaaaa aattctacaa aagtgaattt gcgaacatta gaataggcaa
     5641 cagaattatc aatcgagctt ttgaagaaaa tctaccaatt tatcattgct ctcgagctgg
     5701 agttttttgc gcaaacgata tcggctcatt tctatggctt ttaccgaaac attatgcagt
     5761 gatgcaattt ccttcgatga aaggttgata cgtaataagc cagcgagctc cttttcgtgc
     5821 aaagtaagct gagggcagat attatttagc ttttcgtaga aacttcgatt ggcatcatct
     5881 atttctttct tgaacttatc caactctttg ttgatgttgc cctgttgggt agttttttgc
     5941 cagagttcct tcatcacttt gtccttttgg ctttcattgc tgtttttcag ttcttttaag
     6001 aggtttttca attcgttgat gaattcattt ttttgagcaa taatcagtgc taagttggtg
     6061 agatgattgc tcttgtattt taattcctcg ataagtcgtt gtttttcgag atcttgattt
     6121 cttaactgtg tttctatcag agtttctttt gccttactga tttccgcttc tttcaatagt
     6181 ttttcagctt ctatcttttg cttttctttg attcgtttga agatcagata ggtaataagg
     6241 cccgttaaaa tgagagctaa taatgatgaa atagaaatta ctctttgcaa ggtttgattt
     6301 ttttcgagag tctttcgttt ttcttgttca agaagcagtt cgttttgctg tttttcgaaa
     6361 tcataagttg cctggatgct gaggatttgt ttctgacgtt cgatattatt cagtgtatcg
     6421 ctcaagattg aatacagttt ttggtattga aaggctttga agtaatctcc tgttctttca
     6481 tagatatcgg aaagatattt agcggctgat tttcgaatgg gaatcatgcc atagttcgag
     6541 gccatcttct ctgctttaag aagtaagtcg atggcaaggg atgtatggtt ttgatttgct
     6601 tccaagagtc cttgttcaag tagaactcgg gcaagatttt cctcgtcgtc gatttgttcg
     6661 aaaatttttt ttgcttcttc cagataaatg cgggcatgtg agtgcttgcc cctttcttcc
     6721 gataaaatgc caaggtttag atagtttatg cccatttctt gcttgttgga gagtaattta
     6781 ttaatttcga gacttttctt gtaatagaat tcagccgagt cggtttcgtt gaatatccgt
     6841 tttaaattgc caaaatcgtt gtatatggta gcgaggcctg ctttattgtc gagagattta
     6901 tgtacttgca aagagttttt gaagaagcgg taggcattga tgtagtcgct gttttcgata
     6961 tatattttgc ctatggtttg ataagcattg gctatcatga gtttttcgcc gagtttttcg
     7021 tagattttaa cggctttaat caaatactcg agcgcttttt cgtaaatccc ctgtttttga
     7081 agaatctggc tcatgtcgga atataaacta gcttgaagct gcgtattatt gcacataatg
     7141 gccaatgtaa ttcctctttg catttcctgc agagccagtt tgaatctgcc cttgttctgc
     7201 catcccaagg ccagcaagcg gtgaaggtcg gcattttgtt ctaaaagtcg ataatattcg
     7261 gggctttgtg gttcctgatt ctttatcatt tcgtcggaaa tgctgatggc ttttgcaaat
     7321 tcttgaattg ccgaatcata catacggttc tgaaaataca acaatcctaa attctgcatg
     7381 gatcgaatat aatcgaacca tgaaccatat tttttgttca acgttttatt gatctttgca
     7441 tagtaaatag ccgtatccag attttttccc atgaggctga tgttggacaa ccggctatat
     7501 atcttagcaa ttttcaggga attcaaaccc atagaaaaat cgaggcattg caaataacaa
     7561 ttacgagctt cagtgtaatt tcccgtatag aaatagtaat ctcccagcaa cagcattgct
     7621 tcgtgctgtc ctaatgtata attattcttg cttgctattt cgagtgcaag ttgtgcagcc
     7681 cttcggccaa gatccggatt gttcatcaga taaacatggg ctgcctcgtt tagaatgttt
     7741 actctgacag tatcatgtct aacttttcca agtcgctttt cgagagtatc gggcagtttt
     7801 atttcggttt ctgcatagag aaaaatgggt aacaaggaga gaaacaaaca agcaaaggtt
     7861 ctacacttca tcgagtaaac gcatcattga acatatagat tgattatttt gctgatggct
     7921 tcctgacata ctttgcagaa tgcctgccgg ttgcgggtaa acatgataca gtcggtttcg
     7981 ggcctgtaca ttccatattg gttataccct gcaccttcga atgcacctac tttgcctatg
     8041 tattccatat tgttgatcaa ttgagtttcg aatttgcgtt cccgttcgaa cagggcttcc
     8101 atgacttctt ctggttcgtt gcgagcacgg atttcatgcc gttcgatctg tatctggtaa
     8161 ctgaatttat cgaaggtttc tttgttccat ggggttggaa ttggagtgga aggatctacc
     8221 agatgtttcc atttgaggtt attggggtct ttgagtgcgg taatgttggg ttcccagggt
     8281 tcgatttcga tggatggacc ctcgtatgat accgaagaac tataatactc gtcggtcaaa
     8341 gccgcaaatt gatgcccaaa ttcatggacc attatgtaat cggcaaattt gttgtcggcc
     8401 gaaaccgtag aatagagatt gtagatgcct ccacctccgt atgttctttc attcaccaag
     8461 atgatcatga attcataagg aaccaacgaa gccacttcgc gaatgcttcg gttgtcgaac
     8521 cccagaacat atctttccga atcgaaagct ccgtaggtca gtgaaagagg ggtgcgggca
     8581 tatacaccgg gatggggttt gttgatgcca gcttgctggc aaggtgtaaa aacagcccgg
     8641 acattgaaat cggatttccg gctttgaaag ggttctacgg caaataattc ggataccagt
     8701 ctattgacat ctgccctgaa tttttccatc tgatcatagc ggtaaccatc gcctaaaacg
     8761 acgatatcta ctttataatg cggatcgcca ttttcgatta ctgtccatga tttgaaggga
     8821 agttcgggtt ctgctcgatt tacgaatcga gaattgggat cgatttcttt ttcccagatt
     8881 ttatggaaaa aattatcgga tcctcttttg ctgatgacca gtgtgaatgg ttcgagaggc
     8941 catggaaatc ggatggattc atgaaaagag ccatattccg aagcttcggg agttaattgc
     9001 cattcgccga aaatgctggc aaagcctctg ctgtaaatta cctgattttt tcctttatcg
     9061 attacttcaa agaaatacaa ccccagattc atcttgtctt gaagctgtgt gagactgcca
     9121 gcccaatcac catcattcac gatgcggtcg atggcaaata cttccgttac attatttccg
     9181 gtatgaaaat aatctacccg catggtttta ttttgaaaat aatcgctgaa gcgaatgtct
     9241 tcgcttttta cattttgtga aaaaatcgag caaaaaagaa agagaaaaac tgtaaaacga
     9301 tgattcgaca taagtttaag gtttttatga tttattcgaa aatcgaaaat gttaaatttc
     9361 agaatatttt atagagccaa ccgacaaagt tagtactgaa atagaaactt ataaaatcat
     9421 tttgataccc catggtaaaa ctgtactttt gccagtgaaa aagatggtac tgtcaactta
     9481 ttcaaactga tttcgatgga tataaagtta gaagaattta gcctgagcaa aaatgaatcg
     9541 caaaaaggct tattacagaa agttgggatt gtaggctgcg gcatcatggg gcagcaaata
     9601 gctttacaaa cgagtcagta tgggatcgat gtaatcgggt tagatgttga tcagactcga
     9661 attgaagaga ttacacgatg tttggatgat gagctcaatc gaatcattaa tcattggggt
     9721 atgacggctg ccgagaaaaa gctcattttg tcgaggatta cacttactac ccattacgaa
     9781 gatttaaacg actgtgatat agcaatcgag acgattaaat cgaaaaagcc tggtacgagc
     9841 cttagtatga ggcaagaggt atttcacaaa ttagaagaag tattgcgtca tgatgctgtt
     9901 atagcgagta atactgccac cttgatgatt tctgatcttt catctgtact gaaagacccc
     9961 agcagggctg tcggcatgca tttcatttca cctgtaaggg aagtaaagat tgtagaaacc
    10021 gtacggtcta tgcgtacttc gaaagataca tgggatttgg cctgcaagta tatcaggatg
    10081 attgggaaaa aagtaattcc cgtgaatgaa tcacctggca atatcagcac tcgcatgatc
    10141 ataccgttta tcaatgaagc ctgttccatt ttgatggagg gggtagccat ggtagatcaa
    10201 atcgatgaaa ccatgcgcga agccagcgga cacatgatgg gccctttcga aatggccgat
    10261 accataggtt tagacaagat catgcgctgg atggaaaatt tatacaacga atttggtgaa
    10321 attcgctata aaccttcgcc cttgctcaag cgaatggttc gtgccggaat agttggacaa
    10381 cgcgtgggtg aaggatttta caaatggact ccaaagggga aacaaattaa ggaagggcca
    10441 gtttttactc tgggaagaga atcatttttt aatacgtaac gaattatatt tatgaaaata
    10501 attgttttga attgcggaag ttcttccatt aaatatcagc tgttcaatat gccttcggcc
    10561 gaagtgcacg ccaaaggcct ggtagataag atagggctca agggagcttc tataaaacac
    10621 caacgcaacg atggattgga attcaaggtg gaaggagaga tactcgatca ccagaaagga
    10681 atcgaatatg ttcttggaat cttgattcac cccgagtatg gtagcttgaa aggaatacac
    10741 gaagtggatg ctgtcgggca tagggtggtt catgcgggtg aaaagtttgg tggaagtgta
    10801 cgtataaatc aggacgtagt cggtgcattg gaagaatgca ttgatctggc acccttgcac
    10861 aatcctccca atctcgaagg aatttatgcc attacccgac ttcttccgca aattccacag
    10921 gttggcgttt tcgacacggc tttccatcaa accatgcccg attatgtcta tctttatggc
    10981 ttaccctatt cgctctacga aaagtataaa atcagacgat acggatttca tggtagcagt
    11041 cataaatatg tgtccttacg tgctgcgaag attttgggac aggattatga taaattgcgg
    11101 ctgattacct gtcatttagg aaatggagcc agtgtagcgg ctatcaagaa cgggaaatca
    11161 ttcgatactt ctatgggaat gacacctatc gaaggcctga tcatgggtac acgtacgggt
    11221 gatgtcgatg caggggcatt gcttcatata gccgaaaagg aaggaatagg gtttcaaacc
    11281 ctcaacaaac tcattaataa atttagtggt ttgctgggaa tttcaggcat ttcgagcgat
    11341 atgcgcgatt tggaagaagc t
//
LOCUS       DAOORU010000067        11311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7187_length_11311_cov_5.718195, whole genome shotgun sequence.
ACCESSION   DAOORU010000067 DAOORU010000000
VERSION     DAOORU010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7187_length_11311_cov_5.718195"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(468..540)
                     /locus_tag="PLF20_05610"
     tRNA            complement(468..540)
                     /locus_tag="PLF20_05610"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(505..507),aa:Thr,seq:cgt)
     gene            complement(592..1536)
                     /gene="nadA"
                     /locus_tag="PLF20_05615"
     CDS             complement(592..1536)
                     /gene="nadA"
                     /locus_tag="PLF20_05615"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005825202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOX80515.1"
                     /translation="MNTLEIIDKIKTVKKEKKITLLAHYYQIPEIQDLADFIGDSLAL
                     SKKTKEVDSNMIVFCGVHFMAETAKILNPSKKVVVPVLDAGCSLADSCPADVFESFLK
                     DHPDHIVVTYINSTAEIKAMSDLICTSGNAVQVIESLPKDVRIIFAPDKNLGSYINRL
                     TGRNMVLWNGVCQVHDLLTDEIILKMKMENPDAFLIAHPECNQAVLQIADYIGSTNGM
                     LKFIERSQHKKFIVATETGIIHQMKKIAPDKTFLVAPVDETCSCNDCPYMKMNNLENL
                     YQCMVAEYPEIQIDKYIAERALIPIEKMLEISKMAGLI"
     gene            2028..2471
                     /gene="rpiB"
                     /locus_tag="PLF20_05620"
     CDS             2028..2471
                     /gene="rpiB"
                     /locus_tag="PLF20_05620"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOX80516.1"
                     /translation="MSESKILALGSDHAGYDLKQFIKNELIVQGFDIKDFGCYSPDSV
                     DYPDIIHPLAQEINEGRIKRGIILCGTGIGVSIVANKYPGVRAALCCDVERARLSRRH
                     NDANILALGARFISTDEAMQIIRVFLETEFEGGRHQQRVAKIPIS"
     gene            2496..3872
                     /locus_tag="PLF20_05625"
     CDS             2496..3872
                     /locus_tag="PLF20_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HOX80517.1"
                     /translation="MENYAKQFNKRAEKVSFDKEHRHKILHNIEKYNVAFRKGREFYS
                     DLELAKKRAGYYKYKVINDLGEYLIDFEAHFTAHGGKVIWALNANDALNHILEILKKR
                     RATTVVKSKSMTTEEIGLNEVLEKNRIKAVETDLGEFIVQQAGEKPYHIVTPAMHKSK
                     EDIVRLMNDKFELPPDSTPEEITLFVRGYLREQFLQADAGISGANFLIADLGAIALTE
                     NEGNALLSTSIPSLHIVVAGIEKIIPRMDDLDLFWPLLATHGTGQNMTVYNSIIFGPR
                     QENENDGPTEVIVILLDNGRTRVLEKNRQRIALCCIRCGACLNACPVYKNIGGYVYDT
                     TYTGPIGAVITPLMRGLEEYNHLSFASSLCGECTDVCPVKIPLHELLLFNRYEAVKKG
                     HVESWWRYAMRMSRWLFLHRRLMDTGSPRLRQFLVDKFIVPLWGPRRILPKLAPKSFS
                     KQWTDNLE"
     gene            3931..5103
                     /locus_tag="PLF20_05630"
     CDS             3931..5103
                     /locus_tag="PLF20_05630"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HOX80518.1"
                     /translation="MMNTRYRVCFFTIFLLFNGFSYYSIFSQPLSIEELAREKVYNSY
                     DEAFGDPWNCVVLDLSYQNLTFNSERVRNLNNLRVLHLAGNPNVDFEEAFTRKYFGSL
                     PYLQIIDLQENNLKYLPSGLSKFPYLNMFFAGKNQITEIPDYFCRFEYLQVLSLHHNR
                     IRTLPECLGSLDSLQTLDLSGNTSLQLDQVIPILKECRNLRQLYLSECNLDRLPVTIS
                     DLQSLHYLNLSENKLSQPPQYLFHLSHLQELYLSGNNITSLPDDIQFLENLEVLVLKN
                     NQLKTIPNTIKNLHKLVYLDISGNKLANVSDSLFFLKELKELHLASNQLSSLPAAIKE
                     LTQLEVLDVSSNKLTSLPEEVDYCNSLKYINLKGNSINKEKVQRMKDKWKTVQIVF"
     gene            5180..7627
                     /locus_tag="PLF20_05635"
     CDS             5180..7627
                     /locus_tag="PLF20_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOX80519.1"
                     /translation="MKKLTIILPYLLLMFMENYAQEEIKFQLPPPEILQLVDVKPQPA
                     VYIDYHNRYMLLFERKAFKNLEELAQPELRLAGLRIDPQTHAPSRIRYFYGVQLIDLS
                     TGKEVTLKNIPDSLNIANVSFSPDSRKLAFTNTVGRNISLWIIELETGMARMIYHQRL
                     NACMGAPYQWTPDSESLLVSVVPENLPPISESKPLPEGPFVQETRGEIAALRTYQDLL
                     KNPYDEKLFEYYVTASILKIGLDGSRQPFLPPAMYQNLSYSPDGKYILVSTIQKPFSY
                     NVPYSYFSIKYSIHSSSGEFLNDFYLRPVVEDVLPMFDACEKGRRAIQWRNDRPAELC
                     WVEALDNGDPRIETNARDAIFLQNADFKTPSVKIATTLYRFSGIIWANDQLAVLKEYW
                     YKNRRSLTSFISPAFPMSDKKIVFDLSSEDLYADPGDFLTQRNEFNEWILRFSANNKY
                     LYLEGEGYSPEGNRPFLDEFDVSTFSKRRLWQAEGKSNYESIVRVVDPKQPTMITTIE
                     SKYEVPNLFMRTGKKLRQLTFFTSPYGELQSKIKSEKIFYKRNDGTELLGTLYLPATY
                     DPVKEGPLPLLMWAYPREYKDAQRAGQVKDSPHQFVRFHYGSPVFWALRGYAVLDEAS
                     FPIIGENAAQPNDTFIEQLVEDARAAIDYLAKRGIVDHERVAIGGHSYGAFMVANLLA
                     HSDLFAAGIARSGAYNRTLTPFGFQSEERTFWEAPEVYLKMSPFVYADRIKSPLLLIH
                     GEADNNSGTFTLQSERLFAAINGLGGKARLVILPYESHGYSARENILHTLWETDVWLE
                     KYLKNKEMESTPRPIQE"
     gene            complement(7618..8859)
                     /locus_tag="PLF20_05640"
     CDS             complement(7618..8859)
                     /locus_tag="PLF20_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOX80520.1"
                     /translation="MRIGIFEDEYYRYFQPLARLRPIFDLRCGIYLLREKTSIRFPNI
                     PTDYFVRDILKSLMQETYASIRINEVVSSSYLFINARLLADSSFYSFLHSAEDGQYIL
                     IDGELAAARLNATHLSLLAFDEEGLLVFPSDDRLKIISYDKPGSKMAHYIWDLISENG
                     KQLIDDFQLSPGSGYCGGQLFEGVHLIKEENIFIGKNSKIYPGVTISAENGQVFIGDE
                     VTIMPNSYIQGPCSLGEKTLIKAGSKIYGNTSIGHNCKIGGEIEGSIIHSFSNKQHDG
                     FLGHAYLGQWVNLGANTNNSDLKNNYSTIKAYANGALTDTGLLFLGLIMGDHSKTGIN
                     TMFNTGTMVGIMCNIFGADFPPKYIPDFSWGGKNGLELYKIENALQTAHIVMSRRNQT
                     LSAEMENLIKYYYNQALLNYS"
     gene            9010..10512
                     /locus_tag="PLF20_05645"
     CDS             9010..10512
                     /locus_tag="PLF20_05645"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012055757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOX80521.1"
                     /translation="MNENTNLNPNPLVEYLNKPNSEFTKADIIRYIEDNDINMLNFRY
                     VGGDGRLKTLNFVIHDYQHLDTLLSAGERVDGSSLFKDIEAGSSDLYVIPRYRTAFVN
                     PFTEIPTMDILCSYYAKEGTPYEDAPENILRKSHEIFKQRTGLSFEAMGELEYYLIRP
                     RSELYHADDQKGYHESTPFCKGDGLRRQAMLYIAQTGGFIKYGHSEVGNFSLGDFDYE
                     QNEIEFMPVAVEDAADQIVIAKWVLRNLAYRNNVNLTFAPKITVGKAGSGFHIHTRLM
                     KDDENMMIENGQLSDIAKRAIAGYLDLASSLTAFGNQNPTSYFRLVPHQEAPTNICWG
                     DRNRSVLVRVPLGWLGKANMTIDANPLEPSGEYDFSQKQTVEFRSPDGSANVYYLLAG
                     LVVAARHGLEMPDALDYAERTYVDVNIFEEAHKDKLETLRHLPVSCWESAEQLLVQSD
                     IYTRFGVFSPSFIQGLARQLKLYNDKNLRQEIENNSEAILKLVEKYFHCG"
     gene            10600..11175
                     /locus_tag="PLF20_05650"
     CDS             10600..11175
                     /locus_tag="PLF20_05650"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007218055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOX80522.1"
                     /translation="MKHELPPLPWAKNALEPYISQQTIEFHYGKHHQTYVNNLNNLIV
                     GTPFENSNLEDIILRAEGGIFNNGAQVWNHTFYWNCLMPNGRSEPMGRLGEAIKRDFG
                     SFEAFKENFTSAAVSLFGSGWAWLVKNPHGHLEIIQESNAGNPLRWGKTPLLACDVWE
                     HAYYLDKQNRRADYVADFWKIIHWNAVEERL"
BASE COUNT         3507 a         2038 c         2266 g         3500 t
ORIGIN      
        1 gcctctatgg caatcttcgc tttaaattca gccgtaaatg ttcttcttga cttttccata
       61 tgacaaaaat tttaaaatta gtaaattcct gtccagtttt tttagaccat ctcattacat
      121 tcgatcaatt caaccagata gttctcctat gtaataacaa ttttatccac ttctccaatt
      181 cttatttttc tcctataaac aatacgatat catagtactg ttaagagaaa tttgtttcaa
      241 catataacat cgcgtaagga ggttgattac atattttcag tattgcaggc aatgctaagt
      301 atggttaact tttagatatt tatcctttag ttatcttatt gaacggatga tttcatgcat
      361 gcgttaaagt ttatcataga tcatttttta ttcttactca atctttaatc agcgataaac
      421 tttaagtgga taaaaatttg taataaagaa atatggatct tttatcgagc ctcctggggg
      481 attcgaaccc ccggcctgtt cattacgagt gaaccgctct gccagctgag ctaaagaggc
      541 cttcgaagaa tcaatgcaaa attacaaatc tttggctaat tgtagaatta attatataag
      601 accagccatc ttactgattt ctaacatctt ttctataggt atcaaagctc gctccgcaat
      661 gtatttatca atttgaattt caggatattc agcaaccata cactgataaa gattttccaa
      721 attattcatt ttcatataag ggcagtcatt acaggaacaa gtttcatcga caggagcaac
      781 cagaaaagtc ttgtctggcg ctatcttttt catttgatga ataatacccg tttcggtagc
      841 aacgataaat ttcttgtgct ggcttcgctc gataaatttc agcattccat tagtcgatcc
      901 aatataatct gcaatctgta agacggcttg attgcattcg ggatgagcaa tcaaaaaggc
      961 atcgggattt tccatcttca ttttcaaaat gatttcatct gttagcaaat cgtgaacctg
     1021 gcatacacca ttccaaagaa ccatatttct ccctgtcaat cgattaatat aactacccag
     1081 atttttatca ggagcaaata taatcctaac atcttttggc aatgactcta tgacctgaac
     1141 agcatttccc gaagtacata tcaggtctga catggctttg atctcagccg tactattaat
     1201 ataagtaacc acgatatgat cgggatgatc ttttagaaac gattcaaaaa catcggcagg
     1261 acatgaatcg gctaaagaac atcctgcatc aagaacaggg acaactactt tttttgatgg
     1321 attgagaatt tttgcagttt cggccataaa atgaactccg caaaatacaa tcatgttgct
     1381 atccacctcc tttgttttct tcgacaacgc caaactatct ccaataaaat cagctaaatc
     1441 ttgaatttca ggaatctggt aatagtgagc aaggagagta attttttttt cttttttaac
     1501 agtctttatt ttgtcaataa tttctaaagt attcatcatt attaattctt tataagttat
     1561 atgttgataa tataggtctg ttgatagtat tgaaaacttt ttctaattta tttggatttg
     1621 ttagttatta aaagttatta accctttcga tgggaactgt taattataat agtttgattt
     1681 ttattaattt ataaaagtta ttcaatatgg tggataaata tgaatgtgga taagtttcaa
     1741 ttgcaaagtt agtcataagt gtttataatt tggttgaaaa atgtggataa aattgatagt
     1801 ttttaacagt aagaagataa tgaaaggaag aaggtaaatt ttttcacttt aattcacatt
     1861 tcgtttaatt tatatagcat tttattgttg aaaagttatt tatagtggta ttcaaaatat
     1921 tatggatttc tttaaatatt caaaaagatt gatgaatttc aaacccagaa gatatcagaa
     1981 caggtatgaa aagattttgt atttttgtag tataaatttt caacaaaatg tcggaaagta
     2041 aaattttggc attaggcagt gatcatgcgg gatatgattt aaaacaattt attaagaatg
     2101 aattgattgt gcaaggattt gatattaaag attttggttg ttattcacct gatagtgtgg
     2161 attatcctga tattattcat cctttggcac aggagataaa tgaaggaagg attaaaagag
     2221 ggattatatt atgtgggaca gggataggtg tgagtattgt agccaataaa tatcctggtg
     2281 tgagggcagc tttgtgttgt gatgtagaaa gagctcggtt atcgaggcgt cataacgatg
     2341 ccaatatttt agctttaggt gcacggttta tttcgaccga tgaagccatg caaattattc
     2401 gagtatttct cgagactgaa ttcgaaggag gaagacatca acaaagagtg gccaaaattc
     2461 ctatttctta gattcgaatg gaagaaagga taaagatgga gaactatgcg aagcagttta
     2521 ataagcgtgc cgaaaaagta tccttcgaca aagaacaccg acataaaatt cttcataata
     2581 ttgaaaaata taatgttgct tttcgaaagg gcagagagtt ttatagcgat ttagagcttg
     2641 ccaaaaagcg ggcaggatac tataaatata aagttataaa tgatttagga gaatatttaa
     2701 ttgatttcga agcccatttt actgcacatg gtggcaaagt aatctgggct ctcaatgcga
     2761 acgatgctct caaccatatt cttgaaatat taaagaagcg aagagcaaca acagtggtga
     2821 aatcaaaatc gatgactact gaagaaatag gtttgaatga ggttttggaa aaaaatcgca
     2881 ttaaagctgt agaaacggac ttaggcgaat ttattgtgca gcaggcgggt gaaaagcctt
     2941 atcatatcgt tactccggcc atgcataagt caaaagaaga catagtcagg ttgatgaacg
     3001 ataaattcga gctacctcct gatagtacac cagaagaaat cacattgttt gtccgtggtt
     3061 atttacgtga gcagttttta caagccgatg caggaattag tggagccaac ttcttaattg
     3121 ccgatttagg tgctattgcc cttaccgaaa atgaagggaa tgccctattg agtacttcta
     3181 tcccttcatt gcatattgtt gtggctggca tcgaaaagat cattcctagg atggatgatt
     3241 tggatttatt ttggccatta ttagcaacac acgggacggg tcaaaatatg acagtatata
     3301 attccatcat ttttggtccc cggcaggaaa atgaaaacga tggacccacc gaagtaatcg
     3361 ttattttgct tgataacgga cggactcgcg tactcgaaaa gaatcggcag agaattgcct
     3421 tatgctgcat acgctgtggt gcttgcctta atgcctgtcc ggtgtataag aatattggag
     3481 gttatgtgta cgataccact tatactggtc ctataggtgc tgttataacc cctttgatga
     3541 gaggtctcga agaatacaat catcttagct ttgcctcgag tttgtgtgga gaatgcacag
     3601 acgtttgtcc ggttaagata ccattacatg aattattact cttcaacaga tatgaggctg
     3661 tgaaaaaagg ccatgttgaa tcatggtgga ggtatgccat gcgaatgagt cgctggttat
     3721 ttcttcaccg aagattgatg gatacgggta gcccacggct gaggcaattt cttgtcgata
     3781 aatttattgt accattgtgg ggtcctcgta gaattttacc taagcttgcc ccaaaaagtt
     3841 ttagcaaaca atggacagat aatttagaat aagccgaaag gagcgtatac aagataaatt
     3901 attcatattg atcgcaggaa aaatgaattt atgatgaata cacgatatag ggtttgtttt
     3961 tttactatat ttctgttatt taatggcttt tcgtattatt ctatctttag ccagccctta
     4021 tccattgaag aattagctag agaaaaggtt tacaactcgt atgacgaagc atttggagac
     4081 ccatggaatt gtgtagtgct cgatttaagc tatcaaaacc ttacttttaa ttctgagcgt
     4141 gttagaaatc tgaataattt aagagtgctt catctagccg gtaatcctaa tgttgatttc
     4201 gaagaagcct ttactcgaaa gtattttgga agtttgccct atttgcagat aatcgacctg
     4261 caggaaaaca acctcaaata tctcccttcg ggtttatcga agtttcctta cttgaatatg
     4321 ttttttgccg gtaaaaatca aattacagaa atacccgatt atttttgcag atttgaatat
     4381 cttcaagtac tctcacttca tcataataga attcgaactc tgcccgaatg cctcggttcg
     4441 ctcgatagtc tgcaaaccct cgatttatcg ggcaatacat ctttgcagct cgatcaggtt
     4501 attcctattt taaaagaatg caggaatctg cgtcaacttt atttaagtga atgcaatctt
     4561 gatcgtcttc ctgttactat aagcgattta caatcattgc attatttgaa tctttcagaa
     4621 aataagctat cgcagccacc tcaatattta tttcatctgt ctcatttaca ggaactttat
     4681 ctatccggta ataatatcac ctctctgcct gatgacattc aatttttaga aaacctcgaa
     4741 gtgttagtgt tgaaaaacaa ccaattgaaa acaattccta atactataaa aaatctccac
     4801 aagcttgttt atctcgatat ttctgggaat aaactagcca atgtatccga ttctttattt
     4861 tttttgaaag aattgaagga attgcatcta gcatctaatc aactcagttc tttgcctgca
     4921 gcaataaaag aactcactca gctcgaagtg cttgatgtat cctccaataa gctcacgagt
     4981 ttaccagagg aggtagatta ctgtaattca ttaaaataca ttaatttaaa agggaattct
     5041 attaataagg agaaggttca gcgtatgaaa gacaaatgga aaaccgtcca gattgtcttt
     5101 tgatgatgca gatagggaga agggataata catgaattcg ttcaataaga ttcaatgtaa
     5161 ataaaaaact caatgcttta tgaaaaaatt gacgattatt ttaccttatt tattattgat
     5221 gtttatggag aactatgccc aggaggagat aaaatttcag cttccaccac ccgagatatt
     5281 gcaattagtt gatgtaaagc ctcagccggc tgtttatatc gattatcata atcgatacat
     5341 gttgctgttt gagcgcaagg ctttcaagaa tttagaagaa ttagcccagc cagaattaag
     5401 attggccgga ttaagaatcg atccgcaaac tcatgctcca agcaggataa gatattttta
     5461 tggagtgcag ttgatcgacc tttccacggg taaggaagta acattaaaaa atatccccga
     5521 tagcctgaat attgccaatg tcagcttttc acccgacagc agaaagttag catttacgaa
     5581 taccgttggg agaaatattt cattatggat aatcgagctc gaaacaggaa tggccagaat
     5641 gatctatcat caacgtttaa atgcatgtat gggagctcca tatcaatgga cgcctgattc
     5701 cgaatccttg ttggtgagtg tggtgcccga aaatcttcct ccgatatccg aaagcaaacc
     5761 gcttccagaa ggcccttttg ttcaagaaac ccggggagaa atagccgcat tgcggaccta
     5821 tcaggatttg ctcaagaatc catatgatga aaagttattc gagtattatg ttactgcttc
     5881 cattttgaaa attggattag atggttcacg gcagccattt ttacctcctg ccatgtatca
     5941 gaacctgagc tattcgcccg atgggaaata tattctggtc tctaccattc agaaaccatt
     6001 ctcatataat gttccttatt cctatttttc tattaaatat tccattcaca gcagtagtgg
     6061 agaattcttg aatgattttt atttgcgtcc agtagtcgaa gatgttttgc ctatgtttga
     6121 tgcatgtgaa aaagggcgaa gggcaataca atggcgaaat gacaggcccg ccgagctttg
     6181 ctgggtcgaa gcactcgaca atggagaccc cagaatagaa accaatgcaa gagatgctat
     6241 ttttttgcag aatgccgatt ttaaaacccc atcggtaaaa attgcaacta ccctgtatcg
     6301 tttctcgggg atcatctggg caaacgacca gttagctgtc ctgaaggaat attggtataa
     6361 gaatcgtaga agtttgacga gctttatttc gcccgcattt ccaatgtcgg ataaaaagat
     6421 tgttttcgat ctgtcgtcgg aagatctcta tgccgatcca ggagactttc ttacacagag
     6481 aaacgaattc aatgaatgga tactgaggtt ttcggcaaac aataaatatc tttatttgga
     6541 aggggaaggc tatagccccg aaggcaatcg tccattttta gatgaattcg atgtttcaac
     6601 ttttagcaag cgacgtttat ggcaggccga aggaaaatcg aactacgaaa gcatcgtcag
     6661 ggtagtcgat cctaaacaac caaccatgat tactacaata gagagcaaat atgaagttcc
     6721 caatcttttt atgcgcaccg gcaagaaact tcgacagcta acatttttta ccagtcctta
     6781 tggtgaactt caatcgaaga ttaaatcgga aaagattttt tataagagga atgatggaac
     6841 agagctattg ggtacgcttt atttgccagc tacttacgat ccggtcaagg aagggccttt
     6901 gccattattg atgtgggctt atcccagaga atataaagat gctcagagag cgggacaagt
     6961 gaaagattct ccccatcaat tcgtacgctt tcattatggg tcgccggtat tctgggcatt
     7021 gagaggttat gctgttctcg atgaagcaag ctttcctatt ataggggaaa atgctgccca
     7081 gcctaatgat acttttatcg aacaattggt tgaagatgct cgtgcagcta tagattatct
     7141 tgccaaaaga ggaattgtcg atcatgaaag ggttgccatt ggagggcatt cgtatggagc
     7201 attcatggta gccaatctgc tggctcattc cgatttattt gctgccggga ttgctcgaag
     7261 tggagcctac aaccgaaccc ttactccatt tggtttccag tcggaagaac gaacattctg
     7321 ggaagccccc gaggtttact taaagatgtc gccctttgta tatgccgata ggattaaatc
     7381 cccacttctt ttgattcatg gagaggccga caataattcg ggaactttta ccttgcaaag
     7441 cgaacgcttg tttgcggcta tcaatgggct tggagggaaa gcgcgtttgg ttattctgcc
     7501 ttacgaaagc catggatact ctgccaggga aaatatcctt catactttat gggaaaccga
     7561 tgtctggctc gaaaaatatt tgaagaataa ggaaatggaa tcaactccca gacctattca
     7621 ggagtaattc agtaaagcct gattataata gtatttgata agattttcca tttcggccga
     7681 aagggtctga tttcgcctgc tcattacaat gtgtgcagtt tgtaatgcat tttcgatttt
     7741 gtaaagttcc aatccatttt tacctcccca ggaaaaatcg ggaatgtatt tgggagggaa
     7801 atcggctcca aagatattgc acattatacc caccatagtt ccagtattga acatggtatt
     7861 tattccggtt ttggaatgat cacccataat caagcccaga aaaagcaggc cagtatcagt
     7921 gagtgcacca ttagcataag ctttgatggt cgaatagttg ttttttagat cggaattatt
     7981 ggtgtttgct cccagattca cccactgccc aagataggca tgtccaagaa atccatcatg
     8041 ctgtttgttg ctgaaagaat ggataatgct accttcaatt tctccaccta tcttgcagtt
     8101 atgtccaata cttgtattgc cgtaaatttt tgaacccgcc ttaatcagag ttttttctcc
     8161 aagcgagcaa ggaccctgaa tgtagctgtt tggcataatg gttacttcat caccaataaa
     8221 aacctggcca ttttcggctg aaatagttac accgggataa attttcgaat tttttcctat
     8281 aaatatattt tcttctttga tcaggtgaac cccttcgaat aattgaccac cacaataacc
     8341 gctaccagga ctcaactgaa aatcgtcgat aagctgtttc ccattttcag aaatcaaatc
     8401 ccagatataa tgagccattt tagaacccgg cttgtcgtag gaaataattt ttaatcggtc
     8461 atcacttggg aaaaccagta gcccttcttc atcgaaggcc agcaggctca aatgagtagc
     8521 attcaacctg gctgctgcaa gctcaccatc aattagaata tattggccat cttctgctga
     8581 atgtaggaat gaataaaacg aagaatccgc caataagcga gcattaatga ataaatacga
     8641 cgaagaaact acttcgttta tgcgtatgga agcgtaagtt tcttgcatca gagattttaa
     8701 gatatcacgc acaaaataat ctgttggaat attgggaaat cttatgctgg ttttttctct
     8761 gagcaggtat attccacacc tcagatcgaa tattggtctt aaacgggcta aaggttgaaa
     8821 ataccggtaa tattcatctt caaatatccc aatccgcata aagtataatt tttacaaatg
     8881 taattacata agtggaaata agaatgcaaa tttgaaaaat taagtcctca atgagaattt
     8941 agatgtttct tatcatagaa agtcctaatt ttgctgaaaa ttcaataatt ttaatatatt
     9001 ttaatcgtca tgaatgagaa tacgaactta aatcccaatc ctcttgtaga atatcttaac
     9061 aagccaaaca gcgaattcac gaaggctgat atcattagat atatagagga taatgatatt
     9121 aatatgttga atttcaggta tgttgggggt gatggcagac taaagacatt aaattttgtg
     9181 attcatgatt accagcattt agatactttg ttgagcgctg gtgaaagagt ggatggatca
     9241 agtttgttca aggatataga agcgggttcg agtgatttgt atgttatccc aagatatcgt
     9301 acggcttttg tcaatccatt taccgagata cccacgatgg atattttatg ttcatattat
     9361 gccaaggaag gaactcctta tgaagatgct cctgaaaata tcctgcgtaa atcgcatgaa
     9421 attttcaaac agcggacagg cttaagtttc gaagccatgg gcgagcttga atattacctt
     9481 attcgtccac gttccgaatt atatcatgcc gatgatcaaa aaggatatca tgaatcgact
     9541 ccattttgca aaggagatgg tttgcgcagg caagccatgc tttacattgc tcaaacaggt
     9601 ggttttatca agtatggcca ttctgaagta ggaaactttt ctttgggaga ttttgattac
     9661 gagcagaatg agatcgaatt tatgcctgtg gctgttgaag atgctgccga tcaaattgtg
     9721 atcgccaaat gggttttacg aaatttagca tatcgcaata atgtgaattt gacttttgca
     9781 ccgaagatta cagttgggaa agccggcagt ggatttcata ttcatacccg attgatgaag
     9841 gacgatgaaa acatgatgat tgaaaacggg caattgagcg atattgcaaa gcgtgccatt
     9901 gcagggtatt tagatctggc ttcttcactc acggcttttg gcaatcagaa tcccacttct
     9961 tattttcggt tggtacctca ccaggaggct cctaccaata tttgttgggg cgatagaaac
    10021 cgctcagttc tggtgcgcgt gcctttggga tggttaggga aagccaatat gaccatagat
    10081 gcaaatccct tagaaccctc gggagaatat gatttctcac aaaagcaaac tgttgaattc
    10141 cgaagccccg atggttcggc caatgtatat tatctgttag cagggttggt ggtagcagcc
    10201 cgacatggcc ttgaaatgcc cgatgctttg gattatgccg aacgtactta tgtagatgtc
    10261 aatatcttcg aagaagccca taaggataaa ctcgaaacac tcaggcatct tccggtttcg
    10321 tgctgggaat cggccgaaca gcttcttgta caatccgata tctatacacg gtttggagta
    10381 ttttcacctt cttttatcca aggtcttgcc cgacaattaa agttatataa tgataaaaat
    10441 ttgaggcagg aaatcgaaaa taattcagaa gccatcttga aacttgtcga aaaatatttt
    10501 cattgcggat gattgataac catctaaaga agtttatgta tctttggaag taaaatttac
    10561 aattagttat ctaactaatc atcaaaaaaa ggatgagcca tgaaacatga attaccaccg
    10621 ctgccatggg ccaagaatgc cctggagcca tatatcagtc agcaaaccat tgagtttcac
    10681 tatgggaaac accatcaaac gtatgtaaat aacctcaata atctcattgt gggaacacct
    10741 ttcgaaaatt ccaacctcga agatatcatt ctaagagctg aaggaggaat ttttaacaat
    10801 ggtgctcagg tatggaatca tacgttttat tggaactgtt tgatgccgaa tggtagaagc
    10861 gaacccatgg ggaggcttgg cgaagccatc aaaagagatt ttggttcatt cgaagccttt
    10921 aaggaaaatt tcactagtgc ggctgtttca cttttcggtt cgggttgggc ttggttggtg
    10981 aaaaacccgc atggacatct ggagattatt caggaaagca atgcaggaaa tccgctgcga
    11041 tggggtaaaa ctcctctttt ggcgtgtgat gtatgggaac acgcctatta tctcgacaaa
    11101 cagaatcgcc gtgccgatta tgttgccgat ttctggaaaa tcatccattg gaatgccgtc
    11161 gaagaaaggt tgtgagacct gtatactctg gtttaacttt tatggtttcg actgaaatgt
    11221 agatttattt tcactactga ccgacaaaaa aattgaataa aaagaaggag ggacaaaacc
    11281 gaagtttcat cccccagttg taaatttgtg t
//
LOCUS       DAOORU010000068        11287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7208_length_11287_cov_4.540776, whole genome shotgun sequence.
ACCESSION   DAOORU010000068 DAOORU010000000
VERSION     DAOORU010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7208_length_11287_cov_4.540776"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1103)
                     /locus_tag="PLF20_05655"
     CDS             complement(<1..1103)
                     /locus_tag="PLF20_05655"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOX80523.1"
                     /translation="MSKSYKNQLVFTVKDRCRVCYTCVRECPVKAIRIVNGQAEVMSE
                     RCIGCGNCVRVCSQGAKIFLHSQAEVKELLEGSDPVAALIAPSFPAEFEEISDYHLFI
                     GMVRALGFEYVCEVGFGADMVARRYAEIMNKPTDKGIITSDCPSVTFYIRHYYPHLVD
                     RLAPIVSPMVALNRCVKAEYGENIKTVFIGPCIAKKAESSEVDEALTFTELREMFYEY
                     DITPQNASPSEFDPPYAGKGAIFPVSRGLLQTTNIREDVTERNIIIAEGSPSFKEAVR
                     EFDDGLVSTHHLELLCCKGCIMGAGMGKNGKRYVRRTLVANYVKQKLNQTSQQKREYY
                     VKKFDQLDFSAHFTPEDRRKPLPTKEQIEEALKS"
     gene            complement(1087..2826)
                     /locus_tag="PLF20_05660"
     CDS             complement(1087..2826)
                     /locus_tag="PLF20_05660"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOX80524.1"
                     /translation="MLFSIEVNNKVITAQRGETILECLKRNGVFVPTLCHIEGYSPTG
                     ACRICVVEVEGRPDLVPSCSHPVEEWMKIKTHSPRVIRARRAIAEMLLSNHPDDCLYC
                     GRNGNCELQNLAIDLNLREKRFSGSKNTLKNDYASVSIMREPSKCILCGRCVRLCETE
                     QVVAAVDFIGRGNQMTIGTSYNHGLSSRNCHHCGQCILICPTGALSEQQHIAKVQEAL
                     HNPEKHLVAQFSPALSVGLNENLGIKAGKTADHIIVAALSKIGFSKVFDTGLATDLNI
                     LENASLLAKQIREGESMPMFTSCCPSWLHYAETFHPSILPCISPVLSPQQIMSVLIKK
                     IYASEMRLRAEDIYTVGLMPCTAKKYEILRQEMLIDDLNMTDAVLTTRELARFIKLYG
                     IDVNNIEPMLPHSPFHIRSTAAKLYGVSGGVTEAILRTVYFLLTGVELEPYKIGEVRS
                     GSGPKFFTMEAGKYKVKTAVVSGMSQVQKLLKEIEAGKKNLNFIEVMACPGGCINGGG
                     QKIGASDKDIKTRSKVLYEMDEKENIRVAHKNPIVHELYQKYFDAPLSNKAVEFLHRR
                     RLEKKMEGNEQIL"
     gene            complement(2862..4808)
                     /locus_tag="PLF20_05665"
     CDS             complement(2862..4808)
                     /locus_tag="PLF20_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOX80525.1"
                     /translation="MSKETKKNRNPYIADILLIPPDQYSHQTAERVALLRREKVERPT
                     IFVGAGTCGMIAGASQTMLSIQMYLNDHLMKADVVEVGCIGLCQFEPIVDVQLPGKNR
                     ISFKNITPEKVYSLLDAVFNNTIDSVDVLGQFESRHLVPWEDVPFINRLPFFKKQKRL
                     VLENCGLINPSSIEEYLARGGYKAYAKAIQNYTSTEICEIIEQSGLRGRGGGGYATGK
                     KWMIARQTQSDQKYLICNADESDPGAFMDRALIEGDPHKLIEGIAIAAYAVGASQAFI
                     YIRSEYKKAIQTLKNAIDSAKEFGLIGSNILDSSVNFYLNIKMGSGAFVCGEETALIN
                     NMEGRRGTPEPKPPYPATQGLFSHPTVINNVETLANVPLIIQNGPTWFAGLGTPTSHG
                     TKVFAVTGKVEYNGLVEIEMGRSFREIIYDICGGIKDFKDFKAMQIGGPSGACLGEAC
                     LDLHIDYESLKQEGAMMGSGGLVVMDESACMVDIARFFMNFLQHESCGKCIPCREGTR
                     RMWEILESITKKPVEGQEHQSLERFKGVLMLEELATVVAETSLCGLGKTAPNPVISTL
                     RYFRDEYEEHIFERKCRANVCNGLRTFYIHTEKCTGCTICYRKCPTSAIIGTQRNPHF
                     IVQDKCIGCGICYDVCKFDAVSVH"
     gene            complement(4812..5273)
                     /gene="nuoE"
                     /locus_tag="PLF20_05670"
     CDS             complement(4812..5273)
                     /gene="nuoE"
                     /locus_tag="PLF20_05670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOX80526.1"
                     /translation="MDRVQNIIDRYLGAGKEALLSVLQEIQEEEGYLNAESIHKVSYC
                     LGIPENRIYSIATFYDQFKFSPRARKTIRICEGTACHIMGSSLVAREIKKQIGIEPGQ
                     RTKDNEYELEFVECMGACSQAPAMEINDRYYNKVTPEKLRQILEHLSNFEE"
     gene            5737..5985
                     /locus_tag="PLF20_05675"
     CDS             5737..5985
                     /locus_tag="PLF20_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOX80527.1"
                     /translation="MNEKVEIVICLGSSCFSRGNKHMVKVVDDWLKEKRLKDKVNFHG
                     AHCFSECVNGPVLKIADRIYLQVDTEKVIKVLSEYFNL"
     gene            6012..7889
                     /locus_tag="PLF20_05680"
     CDS             6012..7889
                     /locus_tag="PLF20_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOX80528.1"
                     /translation="MQVIEFDKGKCIECYACVRVCPVKAIAVPINENYPHILHNRCVG
                     CGDCLPVCSPNALSFKNSIDRVKGLLASGEKVAAILAPSIAGEFTDITDYRKFVSMIR
                     ELGFQYVNEVSFGADLVAHEYKELLENFKGKYYITSLCPTLTAYVCYFYPELTVNLAP
                     IVTPMIATARVVKQKYGPEVGVVYIGPCISAKYEPVLLEEENPIDEILTFIELRKMFK
                     EAGITEQTLEYSEFDSPIGHLGSLFPISNGLLQAVGLDENLLTGTITTIEGKDNFIDS
                     VRQFHDYTELIRRHFNIFYCHGCLMGPGTSPGGEKYLRRSLAVEYANKRLKEFDSQSW
                     QENIEKYKTITLSRSFNPDDQRLQSPPKEKIDEVLKVIGRVDADKLMGCGACGFSSCY
                     EFATAVASGLAKPEMCITYNLRNQNEYIKTLKATNEKLAKTEIALKESEKIARREQML
                     AREANEIVNIMLQKLPSGVVIVDENLKIIQANKTFIETLGEDARLIDEVIPGLVGADL
                     KTLLPYHFYNMFSYVLKNAEDITNRDVNFNDNILNVSIFTIRPNKIVGAVVRDLKMPE
                     VRREQIINRINEAITENLEMVQKIGFLLGEGASKTERMLNSIISAYQSENGNKSGETK
                     S"
     gene            7908..9092
                     /locus_tag="PLF20_05685"
     CDS             7908..9092
                     /locus_tag="PLF20_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOX80529.1"
                     /translation="MENNIYIEVSCQQIHHEGEKICGDVFLSRKIKEEGRTIAVLSDG
                     MGHGVKANVLATLTATMALNFTIEHKDVDKIAELIMNTLPVCSERKISYSTFSIVDIQ
                     IDDLTKILEYDNPQCIILRGNDPFQPNWNCVILKSEKNAGKELMTCQFYHHKEDRIVL
                     ISDGVAQSGMGSEKYPFGWGRDNVQDFVVQLLTNDPQISAATLASKVVNFAHINDGYR
                     SKDDTSCAVIYYREPRKLLICTGPPFDEHKDREYAEAVKTFEGKKILCGATTADIIAR
                     ELGVEISDSFEFEDPDLPPISYMPGVDLVTEGILTLEKVNQLLLNYGPNTRLGNGPAD
                     KIVKMLLESDEIFFIIGTRINIAHQDPSLPVELEIRRTVVKRIAQLLEEKFLKEVKLS
                     FI"
     gene            complement(9291..9443)
                     /locus_tag="PLF20_05690"
     CDS             complement(9291..9443)
                     /locus_tag="PLF20_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80530.1"
                     /translation="MGKICDRRKRPNYAANSNLSQHQEDDPIEIRKQFEYRRKNGRLI
                     DGFETF"
     gene            9810..10490
                     /locus_tag="PLF20_05695"
     CDS             9810..10490
                     /locus_tag="PLF20_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008151428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HOX80531.1"
                     /translation="MGGIYIIFIAFAIISWLISNQLKVKFAKYSKMPIQYSGKEIAER
                     MLRQNGIDDVKVLSVEGQLTDHYNPANKTVNLSRDVYYGSNVAAAAVAAHETGHAVQH
                     ASAYAWLQMRSALVPVVSFASRWVQWVLLAGILVVNTFPQLLVAGIILFASTTFFSII
                     TLPVEVNASHRALVWLKGTGITNNQNQAAAFDALKWAAYTYFIAALASLATLVYYVLI
                     LMGARNRD"
     gene            complement(10818..>11287)
                     /locus_tag="PLF20_05700"
     CDS             complement(10818..>11287)
                     /locus_tag="PLF20_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOX80532.1"
                     /translation="FDEDFFAHMEEIDLCWRLKNKGYKIVVIPQSVVFHVGGGTLPKK
                     SWRKTFFNMRNNALMLIKNLPKKWLPIVLFARLFLDFAAALKFLVDGGFKNFWAVFKA
                     YFSLIRSFGRTMSKRENLPSRQVSGIYRGSIVWSYYIRGKRYFNSLKPQKFSK"
BASE COUNT         3338 a         2449 c         2147 g         3353 t
ORIGIN      
        1 gatttcaatg cttcctcgat ttgttctttg gttggcaaag gtttacgtct gtcttcgggg
       61 gtaaaatgtg cactgaaatc gagctgatcg aattttttta cataatactc tcttttttgc
      121 tgactggttt gattcagttt ctgctttaca taattggcca cgagcgttct tctgacgtat
      181 cgcttcccat tttttcccat acctgctccc atgatgcatc ccttgcagca taataattct
      241 aaatgatgag tactgacgag gccatcgtcg aattctctaa cagcttcctt aaagcttggg
      301 ctaccctcgg caataataat attcctttct gttacatcct cgcggatatt cgttgtttgc
      361 agaagccccc tgctcaccgg gaagattgct cctttccctg cataaggagg atcgaattcg
      421 gaaggacttg cattctgagg tgtaatgtcg tactcataaa acatctcgcg aagttcagta
      481 aaggtaagcg cttcgtccac ttcagagctc tcggcctttt tagctataca cgggccaatg
      541 aaaacggttt tgatattttc gccgtattca gcttttacac atctattcaa ggcaaccata
      601 ggcgaaacaa taggcgccag cctatccaca agatggggat aataatgccg gatataaaag
      661 gtaaccgaag ggcaatcgct agtgatgatg cctttgtcgg taggtttgtt cataatttcg
      721 gcatatcttc gggctaccat atcagctcca aaacccactt cacatacata ttcaaatccc
      781 aaagctcgca ccataccaat aaataaatgg taatcgctta tttcttcaaa ttcggccgga
      841 aaactgggcg ctatcaatgc tgcaacagga tcgctcccct cgagcaattc ctttacttcg
      901 gcttgagaat gtaaaaatat cttggctccc tggctgcaaa ctctcacaca atttccacaa
      961 cctatgcacc gctcgctcat tacctcagcc tgcccattga ctatacgtat ggctttcact
     1021 gggcattcgc gtacgcaggt ataacatacc ctacatcgat ccttcaccgt aaaaaccaat
     1081 tgattcttat aggatttgct cattcccttc catttttttt tctaatcttc ttctatgcaa
     1141 aaactcaacg gccttgttac tcaaaggtgc atcaaaatat ttttggtaca gttcatgaac
     1201 aataggattt ttgtgggcta cacggatatt ttccttttcg tccatttcat aaagaacttt
     1261 actacgggtt ttaatatcct tatcactggc gccaattttt tgtcctcctc cattgataca
     1321 gccgccaggg cacgccatca cttctataaa atttaaattt ttcttgcccg cttcaatttc
     1381 tttcaataat ttttgaacct gactcatgcc gcttaccaca gcagttttta ctttgtattt
     1441 tcctgcctcc atggtaaaaa atttgggccc gctgcctgaa cggacttctc caattttata
     1501 aggttcaagc tccacgcccg tcagtaagaa atacaccgta cgtaaaatgg cttcagttac
     1561 tccacccgat actccataaa gtttggcagc cgtacttcga atatgaaacg gactatgtgg
     1621 cagcatgggc tcgatattat tcacatctat tccatacaac ttgataaacc tggctaattc
     1681 acgcgtagta agcacggcat cagtcatatt aaggtcatct atcaacattt cctgcctcaa
     1741 tatctcatac tttttagctg tacaaggcat caacccaaca gtgtaaatat cctccgctct
     1801 cagacgcatt tcgctggcat agattttctt gatcaagacg ctcattatct gctggggcga
     1861 caatacagga ctgatacaag gtaaaatcga gggatgaaag gtttcggcat aatgcaacca
     1921 tgaaggacag caggaagtaa acataggcat gctttctccc tcacgaattt gtttggccaa
     1981 caaagaagca ttttccaaaa tattcaagtc ggtagctaaa cctgtatcga aaaccttaga
     2041 aaagccaatt ttactcagtg ctgcaactat aatatgatcc gcagttttac ctgccttgat
     2101 tccaagattt tcgttaagcc ccacgctcaa tgcaggggaa aattgcgcta ccagatgttt
     2161 ctctggatta tgcaaagctt cttgcacttt tgcaatgtgt tgctgttccg agagagcacc
     2221 cgtaggacag atcaatatac attgcccgca atgatgacaa tttctcgaac ttaatccatg
     2281 attatatgag gttcctatag tcatctggtt gcctctgcca ataaagtcaa cggctgcaac
     2341 cacctgctcg gtttcgcaaa gtctgacaca acggccgcat aatatgcatt tggatggttc
     2401 acgcatgatg ctaacgcttg cataatcatt ctttaaagta ttcttacttc cgctaaaacg
     2461 tttttccctt aaattcagat cgatagcaag gttttgtaat tcacagtttc cattgcgtcc
     2521 acaataaagg caatcgtcgg gatgattaga gagaagcatt tccgctatag ctcttcttgc
     2581 tctgataaca cgaggtgaat gggtttttat tttcatccat tcttccaccg gatgggaaca
     2641 agatggcact aaatcgggcc ggccttccac ttctaccaca catatcctgc aagcacccgt
     2701 tggagaatat ccttcaatat gacaaagcgt gggaacaaat accccattcc ttttcaagca
     2761 ttcaaggatg gtttcacccc tttgggcagt aatgacctta ttattaactt ctatcgaaaa
     2821 caacatggaa cttcttttaa ttatagatat aaacccttga ttcaatgaac agaaactgca
     2881 tcgaatttgc aaacatcata gcatatacca cagccaatac acttgtcctg cacaatgaag
     2941 tgtggattgc gctgagtgcc tataatagcc gaagtaggac acttgcggta gcatattgta
     3001 catccggtac atttttcagt atgaatatag aacgtcctga gtccattgca gacattggct
     3061 ctgcatttac gttcgaaaat atgttcttcg tattcatcac gaaaatatct gagagtgctg
     3121 ataacgggat taggtgcggt tttcccaagt ccacacaaag aagtttcggc aactactgta
     3181 gccaattctt caagcataag aacgcccttg aatctttcga gagattgatg ttcttgacct
     3241 tcaacgggtt tcttggttat cgactcgaga atttcccaca ttctacgcgt tccttctcgg
     3301 catggaatac atttaccaca actttcgtgc tgaagaaaat tcataaagaa ccgtgcaata
     3361 tccaccatac aggcagattc atccataact accaatcctc cactccccat catggcgcct
     3421 tcctgcttaa gcgactcata atcaatgtgg aggtccagac aagcctcacc taagcatgct
     3481 cccgaaggcc cacctatctg catggctttg aaatccttaa aatcctttat ccctccacaa
     3541 atatcataaa taatttcacg aaatgaccta cccatttcta tttctaccaa tccattgtat
     3601 tcaacttttc ccgtaacggc aaagactttc gtgccatggc tagtcggagt tcccaaaccg
     3661 gcaaaccacg tcggaccatt ctggataatc aatggaacat tagccaaagt ttccacatta
     3721 tttatcaccg taggatggct aaaaagaccc tgagtggccg gatatggggg tttgggctcg
     3781 ggagttcctc ttcttccttc catattgttg atcaaagcag tttcttcacc acagacaaag
     3841 gcacccgagc ccatcttgat attcaggtaa aaatttacac tactatccaa aatattgctg
     3901 ccgatcaacc caaattcctt tgcactgtct atcgcatttt ttagagtttg tatggctttt
     3961 ttatattctg aacggatata gataaaagcc tggcttgctc caactgcata tgcagctatg
     4021 gcaattcctt cgataagttt atgtggatcg ccctcaataa gcgccctgtc catgaatgcg
     4081 ccaggatcgc tttcatcggc attgcaaatc agatatttct gatcactttg ggtttgacgg
     4141 gcaatcatcc attttttacc tgtagcataa cccccgcctc cgcgtcccct taatccactc
     4201 tgctcgataa tctcgcaaat ctccgtagac gtatagttct gaattgcttt tgcataagcc
     4261 ttgtatccgc cccttgccag atattcttca atgctcgaag gattgatcaa accacagttt
     4321 tccaaaacca aacgtttctg tttcttgaaa aatggcaagc gattgataaa aggaacatct
     4381 tcccagggaa ctaaatgccg agattcgaat tgccccaaaa catcgactga atcaatagta
     4441 ttgttgaata cagcgtcgag caaagaatag accttctccg gcgtaatatt cttaaaggat
     4501 atcctattct tacctggtaa ttgaacatca acaatgggtt cgaactggca taaacctatg
     4561 caaccgacct ctaccacatc ggccttcatc aaatgatcat tcaggtacat ttgaatgctt
     4621 agcatggttt gacttgctcc agcaatcatt ccgcatgttc cagcacctac gaaaatagtc
     4681 ggcctttcaa ccttttcacg cctcagcaag gccactcttt ctgccgtttg gtgactgtat
     4741 tgatctggtg gaatcaacaa tatgtcggca atatatgggt tccgattttt ttttgtttcc
     4801 ttgctcatgg tctattcttc aaaattggat aagtgttcca gaatctgacg caatttttcg
     4861 ggcgtcactt tattgtaata tcgatcgttg atttccatgg ccggggcttg gctgcatgca
     4921 cccatacact ccacaaactc tagttcgtac tcgttgtcct tcgtcctttg gccgggttca
     4981 ataccgattt gttttttaat ctcacgggct actaaagatg aacccataat atggcatgct
     5041 gttccttcgc aaattcttat cgtctttcgt gctcgtggtg aaaacttgaa ttgatcgtaa
     5101 aatgtagcta tcgaataaat tctgttctcg ggaataccca aacaataact gactttatgt
     5161 atagattcag cgttcaaata cccctcttcc tcctgtattt cctgcagaac tgagagtaag
     5221 gcttctttcc ctgctccaag gtatctatcg attatatttt gaactctatc catttatgaa
     5281 tgttttttta caacatttag aagtttgtta cttgaggcca ctaaattacg aattatttct
     5341 tttattgtta ttgaaataac attgtgaaat acttaacatc aaaaaaatat tcaatttaaa
     5401 acaaaacaac ggaattaata cacttaaaac cagataattg aaagaataag aaggcttatc
     5461 attttttcca ctattcgaaa ccgatcaaga ttctttagat ttgaaagtgt aatggcaatt
     5521 ccgtcgtcct tattataatt catcgaaata aggcattcag cgagtataaa agaaattcag
     5581 tagtcatatt atcggcaaag gaacaagata ttgcataaaa ttggaaacct taggtcaatt
     5641 ttttcaaggc aataaaatta gttcatatac aaaatgtttt acgacaataa aaaagtttac
     5701 ttttgaagaa agatttcaag ttagcgaaag ttactaatga atgagaaggt tgaaattgta
     5761 atttgtctgg ggagttcttg tttttcgaga ggaaataagc acatggtaaa ggtggtagat
     5821 gattggctga aagaaaagcg actgaaggat aaggtaaatt tccatggggc gcattgtttt
     5881 tcggagtgtg ttaacggacc ggtattaaaa atagctgata gaatttatct tcaggtagat
     5941 accgaaaagg taatcaaagt tttatcagaa tactttaatt tataatcaat tgaaaacaca
     6001 ggcgataaga tatgcaggtg attgaattcg ataagggaaa atgtattgag tgttatgcat
     6061 gcgtcagggt ttgcccagta aaagccattg cagtgcccat taacgaaaac tatcctcata
     6121 ttttacacaa tcggtgcgtt gggtgtggag attgtttacc tgtatgctca ccgaatgcgc
     6181 tttcctttaa aaattccatt gacagagtaa aaggattatt ggcttcgggt gaaaaagtag
     6241 ctgcaattct tgctccgagt atagccggag aatttaccga tatcactgat tacaggaaat
     6301 ttgtaagcat gataagagaa ctggggtttc aatacgtgaa cgaagtttcg tttggtgccg
     6361 atctggtagc tcatgaatat aaagaattac tcgaaaattt caaggggaaa tattatatca
     6421 cctctctttg ccccacgctt acggcttatg tatgttattt ctaccccgag cttaccgtaa
     6481 atctggctcc tatcgttacg cctatgattg ctactgctcg tgtggtgaag caaaagtatg
     6541 gaccagaagt aggggttgtt tatatagggc cttgcatttc tgccaaatat gaaccggtgt
     6601 tgctcgaaga agagaatccc atagacgaaa ttctcacttt tattgaattg cgaaaaatgt
     6661 tcaaggaagc gggtatcact gaacaaacgc tggagtattc agaattcgat agccctattg
     6721 gacaccttgg ttccctgttt cccatcagca atggattact tcaagcggtc ggcctcgatg
     6781 aaaacttact cacagggaca attactacta tcgaaggcaa ggataatttc attgattcgg
     6841 tcagacaatt ccacgattac acggaattga tccgaaggca tttcaatata ttttattgtc
     6901 atggctgcct aatgggccct ggcaccagcc caggaggaga aaaatacctc aggcgttcac
     6961 tcgctgtaga atatgccaat aaaagactca aagagttcga ttcccaatca tggcaggaga
     7021 acatcgaaaa atacaaaaca atcacacttt cccgcagttt taaccctgat gaccaaaggc
     7081 ttcaatctcc acccaaagaa aagatcgacg aagtactgaa agtgatagga cgagtagatg
     7141 ccgataaatt gatgggatgc ggagcatgcg ggttttcgtc ttgctatgaa tttgccactg
     7201 cggtagcctc gggattggct aaacctgaaa tgtgcattac gtacaatctt cgcaaccaaa
     7261 acgaatatat taaaacactg aaagccacca acgaaaaact tgccaaaacc gaaattgctc
     7321 tgaaagaatc ggaaaagatt gcccgccgcg aacaaatgtt agcgcgcgaa gccaacgaga
     7381 ttgtaaatat catgttacaa aaactccctt cgggcgtggt catcgtcgat gaaaatctaa
     7441 aaatcattca agcgaataag acattcatag aaactttagg agaagacgct cgcctgattg
     7501 atgaagtaat tcctggcttg gttggagcag atttaaaaac tttgttaccc tatcattttt
     7561 acaatatgtt ttcgtacgtc ttgaaaaatg ctgaagatat taccaatcgg gatgtcaatt
     7621 ttaacgacaa tatactcaat gtttcaatat ttacgataag gccgaacaaa atagtagggg
     7681 ctgtcgtccg cgatctaaaa atgcccgaag taaggcgtga acagattatc aaccggatta
     7741 atgaagccat taccgaaaat ctcgaaatgg ttcaaaaaat tggttttttg ttgggagaag
     7801 gtgccagtaa gaccgaaagg atgcttaatt ccatcatcag cgcatatcaa tcggaaaacg
     7861 gcaataaaag tggagaaaca aaatcctaat atgaagatga ttcatttatg gagaataata
     7921 tatacatcga agtaagttgc cagcagattc atcacgaggg agaaaaaatc tgcggagatg
     7981 ttttcctatc gagaaaaatc aaggaagaag gccgcaccat tgccgttctt tccgatggca
     8041 tggggcatgg cgtgaaggcc aatgttctgg ctactcttac tgccaccatg gcattgaact
     8101 tcaccatcga acacaaggat gtggacaaaa tagctgagct aatcatgaac accttgcctg
     8161 tttgcagtga acgtaaaatc agttacagta ccttttccat cgtagatatt caaattgatg
     8221 atttaaccaa aatcctcgaa tatgataatc ctcaatgtat tattttaagg gggaatgacc
     8281 cttttcagcc caactggaat tgcgtaattc ttaagagtga aaaaaatgca ggtaaagaat
     8341 tgatgacctg tcaattttat caccacaagg aagatcgtat tgttctaata agtgatgggg
     8401 tggctcaatc gggcatgggg tcagaaaaat atccctttgg ttggggaaga gacaatgtgc
     8461 aggattttgt tgttcagctt cttactaatg acccgcaaat ttcagctgcc acactggctt
     8521 ccaaagtcgt caactttgcc cacattaatg acggctatcg cagtaaagac gatacaagct
     8581 gtgctgtgat ctattataga gaaccacgaa aacttctcat atgcacaggg cctccattcg
     8641 acgagcataa agacagagaa tatgccgagg cagtaaaaac cttcgaaggg aaaaaaattc
     8701 tgtgcggagc tactacggcc gatatcattg ctcgtgaact gggagtcgaa atttccgact
     8761 ctttcgaatt tgaagacccc gacttgcctc ctataagtta catgcctgga gtcgatctgg
     8821 ttaccgaagg aattctcaca ctcgaaaaag tcaaccaatt attattgaat tatgggccaa
     8881 atactcgcct tggaaacgga cctgccgata aaatcgtaaa aatgctcctc gaaagcgacg
     8941 aaattttctt cattattggt actcgcatca acattgccca ccaagaccct agccttcctg
     9001 tcgaactcga aatacgccgc accgttgtta aaagaatagc ccaactcctc gaagaaaaat
     9061 tcctcaaaga agtgaagctc tcattcatat aaaaaaaatt gcatgatccc gatcttctat
     9121 cactcaatga ccgctaaacc tttcattaaa atattccctg atgcatttcc cacacattga
     9181 ctcctttttt cggggtgttc aacccaacat accgtatttt tagcctcaag ggctatattg
     9241 aaaatgcagt tttccctgcc atgaatttac ccgacctctt ctccaaaaat ttagaatgtc
     9301 tcaaatccgt caatgagtct tccatttttc cttcgatatt cgaattgttt acgaatttct
     9361 attggatcat cttcctgatg ctgtgaaagg ttactattgg ctgcataatt tggtcgtttt
     9421 cgtctatcgc agattttgcc catggcatgt atgttattat ataagagctg aaatttcgat
     9481 cccgtttaaa aagtttttta ttgtataatc tgtaataaat tatttctcgg tagatatcaa
     9541 aaatcgactg ctgccattcc attatttatt tggaaggcca aataattaga tcgaatcgaa
     9601 atatgttctt cgagctgagc aagtccaaag atgtgaatga aaagcgggct ctttcttatt
     9661 tctatcttcc ttcttatcct gaaaattttc atgaatatta tggtaataaa aatggttttc
     9721 tccgaagatt gaatagaaag gaaatgaggt attctctatc ggatggcata aaacttgata
     9781 atgggggcgt aataattcag atcgtagaaa tgggtggaat atatatcatt ttcattgctt
     9841 ttgcaataat tagctggctg atcagcaatc agttgaaggt taaatttgct aaatattcaa
     9901 aaatgcctat tcaatatagc gggaaagaaa ttgccgaacg tatgcttagg cagaacggga
     9961 tagatgatgt gaaagtatta tcggttgaag ggcaattgac cgatcactat aatcctgcga
    10021 ataaaacagt aaatctgagc cgggatgtat attatggcag taatgtagct gcggctgccg
    10081 ttgcggctca cgaaactggg catgccgtac agcacgcttc ggcttatgcc tggcttcaga
    10141 tgcgttcagc tcttgtgcct gtagttagtt ttgccagccg atgggttcaa tgggtattgc
    10201 tggcaggaat tctggtggta aatacttttc cccaacttct ggtggcaggc attattcttt
    10261 ttgcaagtac tacttttttc agcatcatta ctcttcctgt cgaggtaaac gccagtcaca
    10321 gggcattggt ttggctcaag ggaacaggca taacaaataa tcaaaatcaa gctgcagcat
    10381 ttgatgcctt gaaatgggct gcctatactt attttatagc cgctctggca tcacttgcta
    10441 ctttggtgta ttatgtcctg attctgatgg gtgctcgaaa ccgtgactaa ttgtcaatcc
    10501 atcattttta gaaccattct atgcttgaat ttaatatcaa acttcctata aaggaaggat
    10561 ttcataaccc ttgatcctat cattgcatat tgattccttc agtaatgagg ggataaatca
    10621 tgctttagaa tatcccaaga ataacgagtc tcatcgttct tttgcccttt acgctttcgc
    10681 gagcgatgtt tttcttgcaa agacgccaag aacgctaaga aaatcacttc aaatccttag
    10741 cgaactttgc gtaaagccat ctatccatca tcaaaaccat gacaactccg tttaaaactg
    10801 aatcaggagt tctgaattta ttttgaaaat ttttgaggct tcagggaatt gaaatagcgt
    10861 ttacctctta tataatagct ccacacaata cttccgcggt aaattcccga aacctgtcgg
    10921 gaaggcaagt tttctctttt gctcatagta cggccaaagg atcgaatcaa gctaaaatag
    10981 gctttaaaaa ctgcccaaaa atttttaaaa ccaccatcga ccagaaactt caaagcagcg
    11041 gcaaaatcaa gaaacaaacg agcaaacaaa actatgggta gccatttttt aggcagattt
    11101 tttatcaaca ttaacgcatt gttgcgcata ttgaaaaaag tttttctcca agattttttt
    11161 ggcaaagtgc cgcctcctac atgaaatact accgactggg gtataaccac aattttatag
    11221 cctttattct tcaatcgcca gcaaagatcg atttcttcca tgtgtgcgaa aaaatcttca
    11281 tcaaacc
//
LOCUS       DAOORU010000069        11153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7311_length_11153_cov_5.918634, whole genome shotgun sequence.
ACCESSION   DAOORU010000069 DAOORU010000000
VERSION     DAOORU010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11153
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7311_length_11153_cov_5.918634"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            809..1450
                     /gene="pyrE"
                     /locus_tag="PLF20_05705"
     CDS             809..1450
                     /gene="pyrE"
                     /locus_tag="PLF20_05705"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOX80533.1"
                     /translation="MTGNQEVAKKAAENLLQIKAIKLNLNQSFTWASGWKSPIYCDNR
                     ITLSYPRIRTFIRQEMVKLINEEFGLPDVIAGVATSGIPYGVLVAQDLGLPFIYVRQS
                     EKTHGLGNVIEGVVEDGQSVLVIEDLISTGGTSLKAVETLRKAGCTVKGMVAIFSYNF
                     AVSNEAFKEAKIRLLTLTDYDILIQQALENDYITQKDMESLIAWRQRPEKWGE"
     gene            1447..1842
                     /locus_tag="PLF20_05710"
     CDS             1447..1842
                     /locus_tag="PLF20_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80534.1"
                     /translation="MMNLKSNTIEVRHCPEDLFAYLTDLNRLQQALPPQVINYHVEGD
                     ACSFTIEGMTDISLLISMKIPYTRVVYSNAVDKPFSFSLVFDISPAAENKSMVSVAFE
                     ARANSFLSGILKKPMQNFVDLVSQKLNEL"
     gene            complement(1852..2586)
                     /locus_tag="PLF20_05715"
     CDS             complement(1852..2586)
                     /locus_tag="PLF20_05715"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOX80535.1"
                     /translation="MNLKMIHISSIDSTNNYAARLLSENQMSDNTLIIADYQEQGRGH
                     GSNRWFSRPRKNLLASLYLIHTDILAVDQFVVNCIVSSAIADFLENELKREVKIKWPN
                     DIYVNRQKIAGLLIESFITGERLTSSIIGIGLNVNERDFPPDIPNPTSMFLQDNQLRN
                     PSQVGISITNKILEMHTDKNQYSRARNIYLDRLYQKGVEANYLLLGKKIEAVITGIDN
                     FGRLILRKGTYEYHCNFKEVVYLHNV"
     gene            2674..3051
                     /gene="rsfS"
                     /locus_tag="PLF20_05720"
     CDS             2674..3051
                     /gene="rsfS"
                     /locus_tag="PLF20_05720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOX80536.1"
                     /translation="MAKRKRLADDQAILQAIIEAMHEKKAVDILTINFSEIKNTICDY
                     FVICHGMSKPQVEAISDSVYGKLRNTYSIHPQAIEGIHNSEWILLDYGSIVVHIFQEY
                     TRHFYRLEDLWADAKFIYYDNIA"
     gene            3057..5141
                     /gene="ftsH"
                     /locus_tag="PLF20_05725"
     CDS             3057..5141
                     /gene="ftsH"
                     /locus_tag="PLF20_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOX80537.1"
                     /translation="MNNNNNFSDNNPKGKQTGNVKRKFNYNWIYLLLFAVFFVMWFMN
                     SSDRPKDITWGKLRQLIVEQKVDKIIIVNKENAEIFLKLDSLAAKDKGGKKAQSKGAD
                     FIINVGSLETFEKNLMEAQANIPEDQRIYPQYETRKNWGLDLLGYLIPFILLLALWFL
                     LMRMMSKGSGGAGGQIFNIGKSKAQLFDKDTHVNVTFDDVAGLEGAKLEIMEVVDFLK
                     NPKKYTELGGKIPKGVLLVGPPGTGKTLLAKAVAGEARVPFFSLSGSDFVEMFVGVGA
                     SRVRDLFKQAKEKAPCIVFIDEIDAVGRARGRSPITGANDERENTLNQLLTEMDGFAS
                     NTGVIILAATNRADILDRALLRAGRFDRQIQIELPDLIEREDIFRVHLKPIKIDDTVD
                     AAFLARQTPGFSGADIANVCNEAALIAARKNKKKVDRQDFLDAIDRIIGGLEKRNKII
                     TPEEKRVIAYHEAGHAAVSWLLKYAHPLVKVTIVPRGKALGAAWYLPEERQITTFEQM
                     FDEITATLGGRASEKINFGEISTGALNDLERVTKQAYNMVAYFGLNERLGNISYYDST
                     GQQEFAFVKPYSEETARIIDEEIKKLVDTAYQRALKILQDNKEKVDKLATRLLEKEVI
                     FREDVEDIFGPRPEGSMDIVIEPEKSVKKERETQPAESPMETKNIEEAPISNRTDIDT
                     NNSSKSSNFKDR"
     gene            5146..5814
                     /locus_tag="PLF20_05730"
     CDS             5146..5814
                     /locus_tag="PLF20_05730"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOX80538.1"
                     /translation="MDEITDKEKLLKNIRNALIDKVENPFHDLDLDSSVYPPIEESPD
                     IYFVRRFIEVGGKFVYCANELELSENLSDLLVNENLFPVFTPDERIRYLLLQNGIDVI
                     HNEDDFYEMKTGITSCEYLVARTGSIVFSSRLSSGRRMITVPEVHIVIAHTSQIVVEI
                     KEALANIRLKYQKQLPSFITFVTGPSRTSDIEKTLVMGAHGPAKLYLFLIEEEIKPIF
                     ENLL"
     gene            5834..6040
                     /locus_tag="PLF20_05735"
     CDS             5834..6040
                     /locus_tag="PLF20_05735"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HOX80539.1"
                     /translation="MEENEFALVYTASRNYQALLVKGLLEENGIEASVINKQDSELII
                     GFAEVYVKREDKEKALQIIRSREI"
     gene            6030..6872
                     /locus_tag="PLF20_05740"
     CDS             6030..6872
                     /locus_tag="PLF20_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HOX80540.1"
                     /translation="MKYKTLLVRSATGALYVAMVVFSIALGPWWFAALMFIFFLLSLL
                     EYLHLVLPSKEKILKCLTIIVSSGLYCILCFTALNPLLQTEKLLAWSILLIPILLTFS
                     LFIKSSDILMKIALVLLGIIYITFPFVLLNFIGNPWLTKFDDPRIILLSFFIFVWTND
                     TFAYLMGVNFGRHRLFERLSPAKTWEGMLGGIFFTLVMGILLSCFFKELSFIQWIGMA
                     LITSVSGILGDLSESLIKRTFGVKDSGKAIPGHGGFLDRFDSILFAAPSIFVYFIMIS
                     LITL"
     gene            6912..7580
                     /locus_tag="PLF20_05745"
     CDS             6912..7580
                     /locus_tag="PLF20_05745"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HOX80541.1"
                     /translation="MSKIHPEALKIVPPIALVLLIISGVLTFIFRDSLTGIIIVWVLA
                     LVFIFLFTLFFRFPKRPRPQNGRKVYSSADGKVVVIEEVMEKEYFGEKRIQLSVFMSI
                     TNVHINWSPVTGKVIYEKYKPGRFFPAWMPKSSALNEHNTFVVETHSGHQIMVRQIAG
                     AVARRIAVYKRCGDEVKAGDQLGFIRFGSRVDLFLPLGSEIKVNIGQHVQGSRTLLAI
                     LPPA"
     gene            7755..8234
                     /gene="coaD"
                     /locus_tag="PLF20_05750"
     CDS             7755..8234
                     /gene="coaD"
                     /locus_tag="PLF20_05750"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOX80542.1"
                     /translation="MLSTAVFPGSFDPITIGHHGIILRGLDLFETIIIAIGSNADKKS
                     FFSLESRMQMISLLYDDQPRIQVEVFSGLTIDFCRSHGVRFLLRGLRTSADFEYERVV
                     AHANRKLYPDIETVFLLAAPEHSYISSSLVKEILAYGGDASSFLPPGLKLDACLPGK"
     gene            8330..9703
                     /locus_tag="PLF20_05755"
     CDS             8330..9703
                     /locus_tag="PLF20_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOX80543.1"
                     /translation="MSDILLYGGEVVITGNFSKAAGEQVQVFTYSDFLSLNETLLAQT
                     TIDTQGYFTLEFQIEALQPILLDIAFYRQIIYVEPQKTYHIQSHRFHVLENGNPYIPR
                     YFIDAEIFSESPSDSIFRGLEYHIYNFYDTTTTRLLNQPRTEFVELFVQKILKNIPES
                     IPENYEKAILFRLASLFPKAQLPSGYSTLDEIPIDYNNYEYFRWMEDFFRKKLLIENS
                     LTASSKVSRALIMAVNKSVTYHQLQDTISKFFGIEKLDAKELYTLVALKIFYSTPMFS
                     NSSILYFLEQIRDDSHIESHKRMAQNLLERFTRLKPGSLLPDKVVKSYKGDSIRLREY
                     SQKPLYIVFARKECLTCLANLEMLRPLSVKFKDQVNFLAIFTSHDTLADARTIEQMNY
                     PWPSVLGGRNYELLRAFEAYALPLEMLISPGGIIAAYPAYRVNEGLEITLQKMLMRAL
                     PSGSLPH"
     gene            complement(9648..10226)
                     /locus_tag="PLF20_05760"
     CDS             complement(9648..10226)
                     /locus_tag="PLF20_05760"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOX80544.1"
                     /translation="MRIIRGKRKAKILHPPMNLDVRPTTDMAKVGLFNVIENAWEMEG
                     LHVLDLFAGTGSISFEFISRGSVEVVAVDNNPRCIDWMNEASKGMGFDNLLLVHTDVF
                     SYLQKPVQQFDIIFADPPYDFSKTTFIPDLIFRNHFLYPGGWLIIEHPKKIDFSHHPF
                     FIKKKNYGRVNFSFFSEEGSQTVERASTSSAK"
     gene            complement(10214..11104)
                     /locus_tag="PLF20_05765"
     CDS             complement(10214..11104)
                     /locus_tag="PLF20_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HOX80545.1"
                     /translation="MNETVKTLYQVSDHSFNSNQTQDYHLSILLSGDGFSYVAGNKEP
                     RRLIELGSFEFVIPLDVNRALRNEKALCLFSNFVIEHAWLGNPFGHSGIWIENRYSTL
                     IPEALYDEHALHEYLSFNLQIPQDYILRSEYIPSIEAWNVFALPAEWQKQIAFILPSV
                     KFHHASSCFIEAIIAHGNIGELNENVFIYPHRFYFEMAYTVKNKLIFYNTFPYSTQED
                     FIYFVLFGFEQLGLNPEIVPVILMGEIDTGSLLHLILIRYVRNVNFASRNKDVIYTYA
                     FDQIPSHYFLNIIYSLQCEL"
BASE COUNT         3366 a         2119 c         2281 g         3387 t
ORIGIN      
        1 ttaattaatt agttatcaaa aagttattaa caatttgagt ttatagacag actctcatta
       61 tggcaaatac ttacaagcaa atacatattc agacattttt agtatgcaaa acagagattg
      121 tcttattcaa agccaatgga aagataaatt ttataaatac atataacaga taatccgtag
      181 taataatcat aaattaatgg caataaatgg aatcccagat tacatgcata ttttcattgg
      241 attacgtgtt ttttcaatca ctggctattt gatgggagga gggtccgaat tcagactttt
      301 taactgtcga agttaggttt acagtgtgag gctggcttag cttgaaggca ctgattaagt
      361 caaacagaat ataatttatt tgaactatgt tgcggcgggc ttacattttt ttgcatactt
      421 tgttgtgtcg ggaaaaaagt atgtaaagtt taggacgatg aactcataaa tctatataat
      481 tttaccaaag ttttgaacgg agcgatttat tatcgatgca cttgatcaag ttgatatatt
      541 attgtgcaca ggtgtgatct aaggaaaata ataatattcg aagatttatt ataaaaaatt
      601 gaatatgaat attataagtt tccctatgaa tattagaatt aatgatttta aaccacaggt
      661 aattatttgg acttatttaa ggataggaaa attattaact ttaagattat tttcttaagg
      721 agaaagattt gaatgatttg ggaaaacttt attacttttg caatgaaatc tcaatcccaa
      781 gagaacaatc ataaagcaga ctaaatttat gacaggtaac caggaagtag cgaagaaggc
      841 agccgaaaac ctgcttcaga tcaaggcgat aaaattaaat ctaaatcagt catttacatg
      901 ggcttcgggc tggaagtctc ccatatattg cgataaccgc atcacccttt cttatccgag
      961 gataagaact tttattcgac aggaaatggt caagttgatc aatgaggaat tcggccttcc
     1021 tgatgtgatt gctggtgtgg ccaccagtgg cataccttat ggagttcttg ttgctcagga
     1081 tttaggactt ccatttattt atgtaaggca atcagaaaag actcatggat tagggaatgt
     1141 gatcgaaggt gtggtagagg atggccaatc ggtattagtg attgaagatt tgatttcgac
     1201 tggagggacg agtctgaagg cagtagaaac tcttcgtaag gctggctgca cagtaaaagg
     1261 tatggtggct attttttcat acaattttgc tgtgtcgaat gaagcattta aagaagccaa
     1321 aatcagactg ctcactctga ccgattacga tattcttata caacaagctc tcgaaaacga
     1381 ttacattacc caaaaagata tggaatcgct cattgcctgg cggcagcggc ctgaaaagtg
     1441 gggagaatga tgaatttgaa aagcaacacc attgaggtca ggcattgccc tgaagatttg
     1501 ttcgcatatt taaccgattt aaatcgttta caacaggcac ttccaccgca ggtcatcaac
     1561 tatcatgtgg aaggtgatgc ttgttcattt accatcgaag gaatgactga tatttccttg
     1621 cttattagta tgaaaatacc ttataccaga gtggtttaca gcaacgctgt cgataaacct
     1681 ttctccttta gcttggtttt cgatatttca cctgctgctg aaaataaatc tatggtttcg
     1741 gtagctttcg aagccagggc caattcattt ctatctggca ttctgaaaaa gcccatgcaa
     1801 aatttcgttg atttggtgtc tcaaaaatta aacgagctct gattcattta gctaaacatt
     1861 gtgtaagtaa accacttctt tgaagttgca atgatattcg tatgttcctt tgcgaagtat
     1921 caggcggcca aaattatcga tgccagtgat gaccgcttca attttctttc ctaatagcaa
     1981 ataattcgcc tcgacgcctt tctggtaaag cctgtcaaga tatatattac gtgctctgga
     2041 atattgattt ttatcggtat gcatctcgag aatcttattt gtaatcgaaa tacctacctg
     2101 actgggattt cttaattgat tatcctgaag aaacatcgag gtagggttgg gaatatcggg
     2161 gggaaaatcc ctttcgttca cgttgaggcc tatcccaatt atgctcgatg tgagccgttc
     2221 acccgtaata aaactctcta tcaataatcc tgctattttt tgtcgattca cgtaaatatc
     2281 gtttggccat ttaatcttga cttccctttt gagttcattt tcgaggaaat cggcaattgc
     2341 cgacgagacg atgcaattta ccacaaattg atcaacagcc agaatgtcgg tatgtattaa
     2401 atataagctg gctagcagat ttttgcgcgg tctgctaaac cagcgattcg aaccgtgtcc
     2461 tcgcccttgt tcctgataat cggctataat aagagtatta tctgacattt gattctccga
     2521 caataatcga gcggcataat tattggtgga atcaattgaa gaaatatgaa tcattttcaa
     2581 attcataaaa ttcttatctt tgatatctgg caaaaataat gctctttaaa aaatcagtaa
     2641 ttttgcaaac tattttcata caaagggatg taaatggcaa aacggaaaag gttagctgac
     2701 gaccaagcaa ttttgcaggc catcatcgag gcaatgcatg aaaaaaaagc agttgacatt
     2761 cttaccatca atttttcgga aataaagaat actatttgtg attatttcgt tatttgccat
     2821 ggtatgagca aacctcaagt agaagccatt tccgattcgg tatatggcaa acttcgaaat
     2881 acttatagca ttcatcccca agctattgaa ggaatacata attccgaatg gattcttctg
     2941 gattatggga gtatagtggt tcatattttt caggaataca cccggcactt ttaccgtttg
     3001 gaagatttat gggctgatgc caagtttatt tactacgata acattgcata atcaacatga
     3061 ataataacaa caatttttcg gataacaatc caaagggcaa acaaactggc aatgtaaagc
     3121 gaaagttcaa ctacaattgg atttatttgc ttctgtttgc agtattcttt gtgatgtggt
     3181 ttatgaattc ctcggatagg cctaaggata ttacctgggg taaactgagg caattaatag
     3241 tagaacagaa ggttgacaag atcatcatcg ttaataagga gaatgctgaa atatttttaa
     3301 aactcgatag cctggctgcg aaagataaag gagggaagaa ggcacagtcg aagggagccg
     3361 attttataat caatgtaggc agcctcgaaa ccttcgagaa gaacctgatg gaagcccagg
     3421 cgaatatccc agaggatcag cggatatatc cccaatatga aacccggaaa aattggggat
     3481 tggacttgct gggttatctc attccattca tcttgctgct ggctctatgg tttcttttga
     3541 tgcgaatgat gagcaaggga agcggaggag ctggcgggca gatattcaat attggcaaat
     3601 cgaaggcaca gttattcgac aaggatactc atgtgaacgt aactttcgat gatgtagccg
     3661 ggttagaagg cgccaagctc gaaattatgg aagtggtcga ttttctgaag aatcccaaaa
     3721 aatataccga attgggtggc aagattccga agggtgtttt gctggtaggc cctccgggaa
     3781 cagggaagac attattggcg aaggcagttg ctggcgaagc tcgtgtacct ttcttctcgc
     3841 tttcaggttc tgattttgtt gaaatgtttg tgggtgtggg tgcttcacgt gttcgcgact
     3901 tattcaagca ggcaaaggaa aaggctccct gtattgtatt tatagatgaa atcgatgcgg
     3961 tgggaagggc gcgtggccgc agtcccatta caggcgccaa cgacgaaaga gaaaatacat
     4021 tgaatcagct tcttaccgaa atggatggtt ttgcctccaa tacaggcgtc atcattcttg
     4081 ctgctaccaa ccgagccgat atactcgata gagcactcct gcgcgctggt cgtttcgacc
     4141 gtcagattca aatcgaatta cccgacctta ttgaaagaga agatatcttc agggttcatt
     4201 taaaacctat aaagatcgat gacactgtgg atgctgcctt tttggctcgt caaactcccg
     4261 gattttctgg ggccgatata gcaaatgtat gtaacgaagc tgccctgatt gctgccagaa
     4321 aaaacaagaa aaaagtcgac cgccaggact ttctcgatgc cattgacaga attatcggcg
     4381 gattggaaaa acgcaataaa attatcacgc ccgaagaaaa aagagtgatt gcctatcacg
     4441 aagctggcca tgctgcggtt agctggttgc tcaaatatgc tcatccattg gtgaaggtaa
     4501 ccatagttcc tcgtggaaag gctctgggtg cagcatggta tctgcccgaa gaaaggcaga
     4561 ttactacctt cgaacaaatg ttcgacgaga ttactgcaac tctgggcggt agagcttcag
     4621 aaaagataaa cttcggcgaa attagtaccg gcgcacttaa cgacctcgaa agagttacaa
     4681 aacaggctta caatatggtg gcttattttg gcctgaacga acgcctcgga aatatatctt
     4741 attacgattc tacaggtcag caagaatttg ctttcgtaaa accctatagt gaagaaacgg
     4801 ctcgaattat cgacgaagaa attaaaaaac tcgtagatac ggcttatcaa cgagctctaa
     4861 aaattcttca ggataacaag gaaaaagtag ataaacttgc tacccgtctt ctcgaaaaag
     4921 aagttatttt cagggaagat gtcgaagata tctttggacc tcgccctgaa ggtagcatgg
     4981 atattgttat tgaacccgaa aaatcggtaa aaaaagaaag agaaacccag ccagccgaaa
     5041 gccccatgga aacgaaaaat atagaagagg ctcccatttc gaatcgaact gacatcgata
     5101 caaacaacag tagcaaatct tccaacttca aagatcgatg aattcatgga cgaaattaca
     5161 gacaaggaaa aactcctgaa aaatatcaga aatgctctga tcgataaggt ggaaaatcct
     5221 tttcatgacc tcgacttgga ttcttcggta tatccgccca ttgaagaatc tcccgatatt
     5281 tattttgttc gccgtttcat cgaagttggg ggaaaattcg tctattgtgc caatgaactg
     5341 gaactctccg aaaacctgtc cgatttattg gtcaacgaaa atttgtttcc cgtttttacg
     5401 cccgatgaaa gaatccgtta tctcctgctt caaaacggaa ttgatgttat tcacaatgaa
     5461 gatgacttct acgagatgaa aacgggtatt acttcatgcg aatatctcgt ggctcggaca
     5521 ggttcgatag tattttcatc gaggttgagt tcgggcagaa gaatgataac agtaccagaa
     5581 gtgcatatcg ttattgccca tacttcgcaa atagttgtcg aaataaagga agcccttgca
     5641 aatataagac tcaaatatca aaagcagctc ccttctttta tcacctttgt taccggacca
     5701 agccgcacat ccgacataga aaaaaccctc gtcatgggag cccatgggcc tgctaaactt
     5761 tatctcttcc ttatcgaaga ggaaataaaa ccaatctttg aaaatttgtt atagaatcta
     5821 aaataattgt tgcatggaag aaaatgaatt tgctctggtt tacacggcta gccgtaatta
     5881 tcaggctctt ctggtcaaag gactcttaga agaaaatgga atagaagcca gtgtgatcaa
     5941 caaacaggat tctgaactca ttataggttt tgccgaagtt tatgtaaaac gagaagataa
     6001 agaaaaagct ctgcaaatca ttcgatcacg tgaaatataa aactttattg gtcaggtcgg
     6061 ctaccggagc attgtatgta gccatggtag tgttttccat cgctttggga ccctggtggt
     6121 ttgcagccct gatgttcatt ttctttttat tatccctgct tgaatatctt catctggttt
     6181 taccatcgaa ggagaagatt ctgaaatgct tgacgattat agttagcagt ggactttact
     6241 gcattctctg cttcacagcc ttgaatcctc ttttgcaaac agaaaagtta cttgcctggt
     6301 cgatcctatt gatacctatt ttgttgacct tttcactctt tattaaaagc tctgatattc
     6361 tgatgaagat tgctctggtc ttattaggta tcatctatat tacctttcct tttgtgttgc
     6421 ttaatttcat tgggaatcca tggcttacca aattcgatga tcctcggatt atacttttga
     6481 gtttttttat tttcgtttgg acgaatgata cctttgctta cttgatggga gtaaattttg
     6541 ggcgtcatcg tctcttcgaa aggctcagcc ccgctaaaac atgggaagga atgttgggag
     6601 gaattttttt tactcttgtt atgggcatcc tgttatcctg tttcttcaaa gaactttcgt
     6661 ttatacaatg gataggaatg gctctgatta cttccgtttc tgggatactt ggcgatttat
     6721 ctgagagctt gataaaacgg actttcggtg tcaaagatag tggtaaagca atacctggtc
     6781 atggaggttt tctcgatcgt ttcgattcca tcctgtttgc tgctccatct atttttgttt
     6841 attttatcat gatatcgctc attacgttat aattttgcaa tcgtaaaatc ttattatacc
     6901 gaaaacaagg aatgtcaaaa attcatcctg aagcattgaa aatagttcca cctatagcct
     6961 tggtattgct gattatttcg ggagtgctta ctttcatttt ccgagatagt ttaacgggaa
     7021 ttatcatcgt ttgggtattg gctcttgttt ttatctttct ttttactcta ttttttaggt
     7081 ttcccaaaag gcctcgaccg caaaatggga gaaaggtcta cagttcggcc gatggaaaag
     7141 ttgtcgtgat cgaagaagta atggaaaaag aatattttgg tgagaaacga atccaattat
     7201 ctgtctttat gtcgattacc aatgtacata tcaattggag ccctgtaact ggcaaggtta
     7261 tctatgaaaa atataaaccc ggaagattct tccctgcttg gatgccaaaa tcttcggcat
     7321 tgaatgagca caataccttt gtagtagaga ctcattctgg acatcaaatc atggtgcgtc
     7381 aaattgcagg agcagttgcc cgacgcatag ctgtttacaa acgttgtggc gatgaggtca
     7441 aagccggcga tcagttgggc ttcatccgct ttggctcccg agtcgaccta tttttgcccc
     7501 tgggttccga aataaaagtg aatatcggac agcatgtaca aggcagccga acgcttctgg
     7561 ccattttgcc accagcatga atcaccttct tcagaaactt tctaccttaa ttcccaatca
     7621 gattgcaaat tataattctc aataggatat cattcaaaac taatgtgcaa tgaggcagct
     7681 actggaattc aattcatcaa atgatatgaa acacaaaaat cgtatttctt catcatatat
     7741 aatcctgtaa aacaatgctt tctactgctg tttttcctgg ttcgttcgat ccgattacaa
     7801 ttggccatca cggaattatt cttcgcggac tcgatttgtt cgaaaccatc ataattgcca
     7861 taggatcgaa tgcagataag aaatcattct ttagcctcga gtcaagaatg caaatgatta
     7921 gcctgcttta tgatgatcag ccccgaatac aagtcgaagt tttttcgggc cttactattg
     7981 atttttgccg ttcacatggg gtgaggttcc ttttgagagg cttacgaacc tcggccgact
     8041 tcgagtatga acgtgtcgtt gcccatgcca atcgaaaact ttatcccgat atcgaaaccg
     8101 tatttttgct tgccgcacct gaacactcct atattagttc ttcactggtg aaagagatac
     8161 tggcctatgg cggtgatgca agctcgtttc tccctcctgg tttgaaattg gacgcttgtc
     8221 tacccggcaa atgaattttt ttaaaatttc tattttcgaa aatttcattc atcttcatcg
     8281 gaaagcgaga aaaccaaaaa gaattttatt ggttttaggg cttatttttt tgtctgatat
     8341 attactttat ggcggggaag tcgtcattac aggaaatttt agcaaagcag ccggtgaaca
     8401 ggtgcaagtt tttacttatt ccgatttcct gagtttgaat gaaacccttc tggcccaaac
     8461 taccattgat acgcaaggtt attttacact cgaatttcag atagaagccc ttcagcctat
     8521 cctgctcgat atcgcgtttt atcgtcagat catttatgtt gaacctcaaa aaacttacca
     8581 cattcaatcc catcggtttc atgttctcga aaatgggaat ccctatatcc ctcgatactt
     8641 catcgatgct gaaatttttt ctgaaagccc ttctgattca atatttcgtg gattggaata
     8701 tcatatctat aatttttacg atacgactac tactagatta ttgaatcagc ctcgaactga
     8761 atttgtcgaa ctttttgttc agaagatttt gaaaaacatc cccgaaagca ttcctgaaaa
     8821 ttacgaaaag gctattcttt ttagattagc atcattgttt cccaaggctc aacttccttc
     8881 cggatattcc accttagacg aaattccaat cgattataac aattatgaat attttcgatg
     8941 gatggaagat ttttttcgaa agaagcttct catagaaaat tcactgacag ccagttcgaa
     9001 ggtttcccgg gctttgatta tggcggtaaa taaatcggta acttatcacc agcttcagga
     9061 taccatatcg aaattctttg gtatcgaaaa actcgatgca aaagagttat atactttggt
     9121 ggctttgaaa atattttatt ccacccctat gttttccaac tccagtatcc tttattttct
     9181 ggagcagata cgggatgatt cgcacataga gagtcataag cgaatggctc agaatctact
     9241 cgaaagattt acaagattga aacccgggag tttgctacct gataaagttg tgaaaagtta
     9301 taagggagac agtattcgat tgcgcgaata tagccagaaa ccattgtata ttgtatttgc
     9361 acgtaaggaa tgcctgacat gccttgcgaa tctcgaaatg ctaaggcccc tttcagtaaa
     9421 attcaaggat caagtaaatt ttttggccat ttttacaagt catgatacac ttgcagatgc
     9481 tcgaacaata gagcagatga attatccctg gccgagcgta ttaggagggc ggaactacga
     9541 attgcttcga gcttttgagg cctatgcctt accacttgaa atgctaattt ctcccggagg
     9601 tatcatagct gcttatccag cttaccgggt aaatgaaggt cttgaaatta ctttgcagaa
     9661 gatgttgatg cgcgctctac cgtctgggag ccttcctcac tgaaaaaact aaaatttacc
     9721 cttccataat tcttcttttt tatgaagaat ggatgatgag aaaagtctat tttttttgga
     9781 tgttcgatga tgagccatcc accgggatac aagaaatgat tccgaaaaat caagtctgga
     9841 ataaaggtag ttttcgaaaa gtcataaggg ggatcggcaa aaatgatatc gaattgctgc
     9901 acaggctttt gtaaatagga aaacacgtcg gtatgcacca gcaataaatt atcgaaaccc
     9961 attccttttg aggcttcatt catccaatcg atgcatctag gattattatc gaccgcaacc
    10021 acttcgacac ttcccctcga aatgaactca aacgaaatgc ttcccgtacc tgcaaataaa
    10081 tcgagcacgt gaaggccttc catctcccat gcattttcga tgacattgaa aagccccact
    10141 ttggccatat cggtagttgg ccttacatcg agattcattg gaggatgaag tattttggcc
    10201 tttcgtttcc ctcttataat tcgcattgca aagaatagat aatgttcaga aaataatgag
    10261 aaggtatttg atcaaaagca taggtgtaaa taacatcttt gtttcggctg gcaaaattaa
    10321 catttcgaac gtaacggatg aggatgagat gaagtagtga accggtatcg atttcaccca
    10381 tcagtattac cggcactatc tcaggattca ggccaagctg ttcgaaaccg aatagaacga
    10441 aatagataaa atcttcttgg gtagaataag ggaaggtatt ataaaagatc agtttgtttt
    10501 taacagtata agccatctcg aaataaaagc gatggggata gatgaacacg ttttcattca
    10561 actcaccgat attgccgtgg gctattattg cttcaataaa acacgatgaa gcatgatgaa
    10621 acttaactga aggcaagata aaagcgattt gtttttgcca ttcggctggg agggcaaata
    10681 cattccatgc ttctatggag ggtatatatt cacttcttaa tatataatcc tgaggaattt
    10741 gcaggttgaa gcttaaatat tcgtggaggg catgttcatc gtaaagtgct tcaggaatta
    10801 gcgtggaata acgattttcg atccatattc ctgaatgtcc aaatggattt cctaaccaag
    10861 catgctcgat cacaaaattc gaaaatagac ataaggcctt ttcatttctc aaggctctgt
    10921 ttacatcgag gggtattaca aactcaaaac tgcctaattc aatcagccgg cgaggttctt
    10981 tattcccggc tacatacgaa aatccatccc ccgaaagaag gatggataaa tgatagtctt
    11041 gcgtctgatt ggaattgaaa ctgtggtcgc taacttgata aagggttttt actgtttcat
    11101 tcacatcagc aagttttagt aaccggcctg ggtaggtcta ctcccagttt cca
//
LOCUS       DAOORU010000070        11063 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7383_length_11063_cov_5.119640, whole genome shotgun sequence.
ACCESSION   DAOORU010000070 DAOORU010000000
VERSION     DAOORU010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11063
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7383_length_11063_cov_5.119640"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="PLF20_05770"
     CDS             complement(<1..1146)
                     /locus_tag="PLF20_05770"
                     /inference="COORDINATES: protein motif:HMM:NF015039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HOX80546.1"
                     /translation="MNKKLWIGILLLIPSLLFSQKPVDELRYQSITPKFYRKILADTG
                     LRMTPSSVAFARMVAITQKSMPTNIDLYQRLWHQPSLSQGESGTCWCFAAVSFLESEC
                     NRLTGKEIKLSEMFFVYHEYLERAAQFVKTRGETDFGEGSETSSIPILLKRYGCMPES
                     AYPGKPPTSQFHHHRQMVAEMKSYLKNVKQNNAWDEETVLKVIASILNHYMGQPPASF
                     PYHGKTYDPYSFANEIIGLRPDDYVHFMSNIRQTQNQRGELIENDNWRHFDEYYNISL
                     DEFSNLVHSALEKGYSIVICGDVSEPGFDRTTGTAWIPNFDIPRSYIDASARELRLYN
                     GSTTDDHCMHVVGYKKLNDQWWYLLKDSSSGAFDSPSPGYNYMHEDYV"
     gene            complement(1139..1651)
                     /locus_tag="PLF20_05775"
     CDS             complement(1139..1651)
                     /locus_tag="PLF20_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014223756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HOX80547.1"
                     /translation="MKPKCENDLYPILDQFGIQYHIYRHPPAPTIQIAREFWKDIPSS
                     HCKNIFLRNHKGNQHYLVVLEHTREFSIHSLEKRLHQGKLTFASAKRLMQYLGVTPGN
                     VSPYALINDVEHHVHLFLDGTLLSAKSISFHPLTNTASLVTSFEDFLRFLNWTQNTYE
                     FINLTEDYHE"
     gene            complement(2087..2812)
                     /locus_tag="PLF20_05780"
     CDS             complement(2087..2812)
                     /locus_tag="PLF20_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOX80548.1"
                     /translation="MEEVIRFEDAAIFQRDILVLNGIDLVVKPGEFVYFIGKTGSGKS
                     SLLRTIYADLPLRAGKGWVAGFDLTRLRKRQLPYLRRKIGIVFQDFQLLSDRTVAENL
                     LFVMKATGWKDKKQMKQHIEELLSKTGIPTKGFKMPHQLSGGEQQRVGVARALINQPE
                     ILIADEPTGNLDPESSDGILQLIQEINRSGTTVIMATHNYRLIRDFPGRVIRLEQGKL
                     LETPQTMEEENITENPDNLDNEP"
     gene            3203..3418
                     /locus_tag="PLF20_05785"
     CDS             3203..3418
                     /locus_tag="PLF20_05785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80549.1"
                     /translation="MYLDKESCHIHFGLGARQIQLYNLQGKVIREWLVPQGCIFMDVS
                     LKNLPTGIYLFRVTENQGVKGMRVVKI"
     gene            3534..4928
                     /locus_tag="PLF20_05790"
     CDS             3534..4928
                     /locus_tag="PLF20_05790"
                     /inference="COORDINATES: protein motif:HMM:NF025714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4403 family protein"
                     /protein_id="HOX80550.1"
                     /translation="MKGHFLGLFIIPLAIVVTSCRTLAPEKPIESYPIARQQARSSFI
                     QVNIPLEGVVIQQIINRNFQGLLWADTLPDKDDDNFMMKIWKKDEIRLTIEENIIHWE
                     APLRVWLRTGFKIQKFGLNFSDSRELNTEILLKFSTRVELQPDWTVLTQTQSDGYAWL
                     KSPTMKFSSYDIGITRLANILMQAGQQRLASAVDTLLMQELKIKFYASSLWNAIQQPI
                     KINANPVVWLQMKPLGFYALPFHAQNNNIDYRLGISCILTALVNENPPYQDSVSLPAL
                     SFDFPQNRESIVHTRVEASMKTAEDLANQMLRGQKFKFGQKEFIVRSIKLYGSESKLA
                     VEAQIGGEINGKLYFTGYPYYDTTTQSIRLRDFQFDLKTDNILKDIASWVKRTNIENS
                     LYKLLVFDLSKQIAFLQDVFGSFLNELSRTNHIEINGKINSFSVDDIQISPEIITAYL
                     TAKGEIGVRLLPSS"
     gene            complement(4964..5539)
                     /gene="rsxA"
                     /locus_tag="PLF20_05795"
     CDS             complement(4964..5539)
                     /gene="rsxA"
                     /locus_tag="PLF20_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HOX80551.1"
                     /translation="MEYILIIVSAIIVNNVVLTQFLGICPFLGVSNKLSTAIGMSGAV
                     VFVMTLATIVTFLVHHYILIPLQITFLQTIAYILVIAALVQMVEIILKKISPSLYQAL
                     GIFLPLITTNCAVLGVAILVIQKNFNLMESVVYTIATALGFGLALIIFSGLREQIELN
                     QVPKGMRGTPIALVTAGLLSLAFMGFTGIVK"
     gene            complement(5551..6141)
                     /locus_tag="PLF20_05800"
     CDS             complement(5551..6141)
                     /locus_tag="PLF20_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOX80552.1"
                     /translation="MNQWSNFTKGFIKESPTFALILGMCPTLGVSSAAINGLGMGLAT
                     TFVLAMSNLFISALKDFIPDKVRIPAFIVVIASFVTMVDLAMAAYVPALHKTLGIFIP
                     LIVVNCIVLGRAEAFASRNSVISSLIDGLGMGLGFTFALIMLGCIREALGNGSVFGYK
                     FINPTSNGILIFVLAPGAFITLGFLIAFINRFRKTS"
     gene            complement(6138..6716)
                     /locus_tag="PLF20_05805"
     CDS             complement(6138..6716)
                     /locus_tag="PLF20_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOX80553.1"
                     /translation="MAKKESTLKNMVICLLTITVVASAALAGVYNMTKEPIAAAANQK
                     KIDAIHQVIPDFDRLKRIAYLPPDGKDSVILYYGYKNDSLVGVAVETYSERGFSGHIG
                     LMVGFKADGTINSIEVLEQKETPGLGTKMTEPAFKDQFKGLDISKLPNEQPKVKKDQG
                     TIEAITAATISSRAFCDAVQRAYQAKKRGDEK"
     gene            complement(6718..7707)
                     /locus_tag="PLF20_05810"
     CDS             complement(6718..7707)
                     /locus_tag="PLF20_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005653899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOX80554.1"
                     /translation="MNPLIVSGSPHIHGEMTIKSIMWGVVIALLPAWLAGVWFFGIGA
                     VIVTLVSIASCVLFEFLIQKFVLKQEPTVNDGSAVVTGLLLAMNVPSSLPWWQVVIGS
                     LVAIGIVKMAFGGLGKNLFNPALIARVFLLISFPVTMTTWPLPIESRANVFNFDGVTG
                     PTPLGTLKTQLAMGQTVAQIKLPSYTNLLWGNIGGSFGEVSAIALIIGGIYMLYRKII
                     TWHIPVSFILTAFIFSGILCLVNPNRYADPVFHILAGGLLIGAIFMATDLVTSPMTAW
                     GKIIFGIGCGVFTILIRVFGAYPEGVSFAILIMNAFVPLIDRAFKPKRFGEVK"
     gene            complement(7712..9043)
                     /gene="rsxC"
                     /locus_tag="PLF20_05815"
     CDS             complement(7712..9043)
                     /gene="rsxC"
                     /locus_tag="PLF20_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOX80555.1"
                     /translation="MLKTFKKGGVHPHENKFSANSPIQPIDPPQKIFVPLSQSLGAPS
                     KPVVKKGDEVKAGDILATGESFISSYLHSPVSGKIENIDDFIDVSGYRKPAIFIEVTG
                     SEWNASVNLSDELKEDITLEGASIIQKVKEMGVVGLGGATFPTHVKLMIPQGKKAELL
                     IINGVECEPYLTSDHRLMLEKPREIITGTRILMKALGVTRAIIGIENNKPDAINLLSK
                     HASSYPGIEVCPLRVKYPQGGEKQLIQALTGREVPSGKLPIEVGCIVQNVGTAFAVYQ
                     AVQKNRPLVERVVTVTGTNVLHPSNFLVKIGTPVSALLEKAGWQPEKAGKVIAGGPMM
                     GKALLNADVPIVKGTSGILVLNGKESLRPKVQPCIRCAQCVSACPMGLEPYYLARVSE
                     VEKFDLAENGAILDCIECGSCQYICPSARPLLDHIRLGKLKVNQIIRSRNQ"
     gene            complement(9078..9941)
                     /locus_tag="PLF20_05820"
     CDS             complement(9078..9941)
                     /locus_tag="PLF20_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster domain-containing protein"
                     /protein_id="HOX80556.1"
                     /translation="MNPSILYAVITLAVIGAGSGAILYWVASKFKVEEDPRIDTVAEA
                     LPQANCGGCGYAGCRNFAEAIVKTGSLDNLFCPVGGDEVMKRIASILGLEAPTMQPMI
                     AVVRCNGSFQNSPSKFHYDGVKSCAFAHITFTGEGGCPFGCLGQGDCERACSFDAIHI
                     NPKTGLPEVNDKCTACGACVKACPRNIIEMRPKGKKDRRIFITCMNQEKGAIARKNCE
                     AACIGCNKCVKECRFDAITVSNNLAYIDPHKCTLCRKCVEVCPTNCILEVNFPPRKRT
                     TQSDSPAEITA"
     gene            complement(10234..10653)
                     /locus_tag="PLF20_05825"
     CDS             complement(10234..10653)
                     /locus_tag="PLF20_05825"
                     /inference="COORDINATES: protein motif:HMM:NF016160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOX80557.1"
                     /translation="MSSTISHEGIVTRVAPEGIQVTIISQSACSACHAKGVCSISEMT
                     EKIIDVSVPDFQEYHQGQRVVVEIKESEGSTAVLWGYVIPFIVLVVAFSLLYFLTHNE
                     LYAGLGSILSLIPYYLVLFILRNWMHKKFSFKVHPLE"
     gene            complement(10879..>11063)
                     /locus_tag="PLF20_05830"
     CDS             complement(10879..>11063)
                     /locus_tag="PLF20_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80558.1"
                     /translation="SFLNSSFRTFMAKSSSLLVEITSGFKNSEIWQKGENELRDNVNA
                     HTHFQYCRNYFYRFSL"
BASE COUNT         3358 a         2286 c         2129 g         3290 t
ORIGIN      
        1 tacataatct tcgtgcatgt aattatatcc cggtgaagga gaatcgaaag cacctgagct
       61 cgaatccttc aaaagatacc accattggtc gttcaatttt ttgtaaccca caacatgcat
      121 acagtgatcg tcggtagtgg agccattata caatctcaat tctcttgcag aagcatcaat
      181 gtaggaacga ggaatgtcga aatttggaat ccatgctgtt cctgtagttc tatcgaaacc
      241 tggttcggaa acatctccac aaataactat tgaatagcct ttttcaagag ccgaatgaac
      301 cagattggag aattcatcga gcgaaatatt ataatattca tcgaaatgtc tccaattatc
      361 gttttcgatc aattctccac gttgattttg tgtttgccgg atattcgaca taaaatgcac
      421 ataatcgtcg gggcgaagac ctattatttc attagcaaaa gaatatggat catatgtttt
      481 tccatgatac gggaaagaag ctggaggctg tcccatataa tgattcaaga tagaagctat
      541 gactttcaga acagtttctt catcccatgc attattttgt tttacgtttt ttaaatacga
      601 tttcatttcg gccaccattt gcctgtgatg atgaaactgg gaggtaggag gttttccagg
      661 gtaagccgat tcaggcatgc agccataacg tttcagaaga atgggaatac tggatgtttc
      721 agagccttca ccaaaatcag tttctccacg tgtttttacg aactgagcgg ctcgttcgag
      781 atattcatga taaacgaaaa acatttccga aagcttaatt tctttgcctg tcaaccgatt
      841 acattctgat tcaagaaaag atactgctgc aaaacaccaa catgttcccg attcgccttg
      901 gcttagagaa ggttggtgcc ataacctctg atacaaatca atgtttgtgg gcatggattt
      961 ttgtgtaata gccaccatac gtgcaaaggc cacagatgaa ggagtcatcc tcaaacctgt
     1021 atcggctagt atttttcggt aaaatttagg ggtgatgctt tgatatctca attcatcgac
     1081 gggtttctgt gaaaataaca aggatgggat aagcaataag atacctatcc ataatttctt
     1141 attcatgata atcttcggtt aggttgataa attcataggt gttttgagtc cagtttaaaa
     1201 atcgcagaaa atcttcgaaa gaagtaacca gtgaagctgt attggtgagc ggatgaaagc
     1261 taatgctttt agccgaaagg agagttccat ctaaaaaaag atgaacatga tgttccacat
     1321 cattgattaa ggcataagga gaaacgttgc caggggtaac gcccaggtac tgcatcagtc
     1381 gtttggctga ggcaaaggta agttttccct gatgcaagcg tttttcgaga gagtgtatcg
     1441 aaaattcccg ggtatgttcc aaaacaacca gatagtgctg attcccctta tgatttctca
     1501 ggaagatgtt tttgcaatga gaactgggaa tgtctttcca gaattcacga gcaatctgta
     1561 tggtaggagc aggaggatgc cggtaaatat ggtattgaat tccaaattgg tcgagaatgg
     1621 gataaagatc attttcacat ttaggcttca ttcttgaatg aaattccaat agctttcaaa
     1681 attaatcttt tctcgagaaa ttaaattcag acaacacatc ctatcacact ctattgattt
     1741 gcatatattt ttaaattcaa acctttaacc ttttcctact attcaccaac caatcctcta
     1801 aacattaaat cccaaattac aactaacaac gaacaactta ccaaattaca aatttcaatc
     1861 tctgtcacac cgtctccttg tctcttcgtc ttccttcctt ccttccttcc tccattttca
     1921 actttatgaa cttttaactc atccatccac catccaccaa tccagcttgt ctcccggtct
     1981 cctcgtcccg cagtctcctc gtctcccttc ctcccttatg aacttttaac ttaccaatca
     2041 ccatctccta aatactagtt acaaaataaa agttgttttt tatgcttcac ggctcattat
     2101 caagattatc agggttttct gtgatatttt cttcttccat agtttgaggc gtttcgagga
     2161 gttttccctg ttcgaggcgg attacgcgtc ctgggaaatc gcgaataagc cggtaattgt
     2221 gagtggccat gatgacagtg gttccacttc ggttaatctc ctgaattaac tgtagaatgc
     2281 cgtccgacga ttcaggatcg aggttgccgg tgggttcgtc ggcgatgagg atttcgggct
     2341 gattgatgag tgctcgggct acacccacgc gttgttgttc ccctcccgag agttgatggg
     2401 gcatcttaaa gcctttggta gggattccag tctttgatag taactcttct atatgttgtt
     2461 tcatctgttt cttatctttc cagcccgtag ccttcattac aaaaagcaga ttttcggcca
     2521 cagttctgtc ggaaagcaac tgaaaatcct gaaaaacgat tcctatcttt cgacgtagat
     2581 aaggtagctg acgctttcga agccgagtta aatcgaaacc tgctacccat cccttgccag
     2641 ctcgaagtgg caaatcggca taaatggtcc tcaacaaact cgacttgcca ctccctgtct
     2701 tcccaatgaa atacacgaat tctcccggtt ttactaccag atcgattccg ttgagcacaa
     2761 gaatatccct ctgaaatatg gccgcatctt cgaaacgaat cacttcctcc attaaaatcg
     2821 gtatatttta aataattatc acctacaaag atatcagtct tttttgattg ttctttgtat
     2881 taaacaatca tatttggatg tgaatttttc tcaatcgctc ttgtttgagg agatttggca
     2941 atcaaaccat gagcaataac ccaaaacttt ctatcaaagc ttagcctgaa aaaattactt
     3001 ccctccaact tctatctctt tttatcccga aaattttctc taatcgaaag tttatcagcc
     3061 agctatgcat tttctatgga tagccgcaga attcccgttt gctccttgta atgggttctt
     3121 ctctattgga aaattattta tcattaggtt ttaaaattta tttattaagg atcatcatta
     3181 agcaaaagat agaaaactgg atatgtatct tgataaagaa agttgtcata ttcattttgg
     3241 tcttggagca aggcaaattc aactatacaa ccttcaaggt aaagtaataa gagaatggct
     3301 tgtacctcaa ggttgtattt tcatggatgt gtcgttaaaa aatcttccaa cagggattta
     3361 tctattcaga gtaactgaaa atcagggagt gaaaggcatg agagtagtaa aaatatagaa
     3421 caatgattaa aatcaataac atggcatatc actcttatgt gagaggagtt ttaagaactt
     3481 gatttcggta aattttgcaa agtccagtaa atttgtcagc acaataatgt tatatgaagg
     3541 gtcattttct tgggttattt attattccgc tggcaattgt agttacctcg tgccgtacct
     3601 tggctcccga aaagcctatc gaatcgtatc ccattgcaag gcaacaggct cgctcatctt
     3661 ttattcaggt aaacatcccg ctcgaaggtg tagtaatcca acaaatcatc aatcgtaatt
     3721 ttcaggggct attatgggca gatacgcttc ccgacaaaga tgatgataat tttatgatga
     3781 agatttggaa aaaggatgaa atccgattga ctatagaaga aaatattatt cattgggaag
     3841 caccattgag agtttggctc aggacagggt ttaagattca gaaatttggt cttaattttt
     3901 ccgattcccg agaattgaat actgaaattc ttttgaagtt ttctactcga gtagaattac
     3961 aacccgactg gacagttctt acacaaacgc agtcagatgg ctatgcgtgg ctaaaatcgc
     4021 ctacgatgaa atttagcagt tatgatattg gtataacaag gcttgccaac atcctcatgc
     4081 aagcgggtca acaaaggctc gcttctgctg tcgatacatt gctcatgcag gagcttaaaa
     4141 taaagtttta cgcttcgagc ttatggaatg caatacagca gcctataaaa attaatgcta
     4201 atcccgtggt atggctgcaa atgaagccct tagggtttta tgctcttccg tttcatgcgc
     4261 aaaacaacaa tattgattac cgattgggaa tttcgtgtat tctaacggct cttgtaaatg
     4321 agaatccacc ttatcaagac tcggtttcac ttccagcctt aagtttcgat tttccccaaa
     4381 atagagaatc gatagtgcat acacgtgttg aagccagcat gaaaaccgcc gaagatttgg
     4441 ctaatcagat gttaagaggg cagaaattca aatttggcca gaaggaattc atcgttcgat
     4501 ccatcaaatt atatggaagc gaaagcaagc ttgccgtgga agcgcagatc ggtggagaaa
     4561 taaatgggaa actttatttt acgggatatc catattacga taccaccacc cagtctattc
     4621 gtcttcgtga ttttcagttc gacctcaaga cggacaatat attgaaagac attgcttcat
     4681 gggtaaaacg aacgaacata gaaaattcat tgtataaatt actggttttc gacttgagta
     4741 agcaaatagc ttttttgcaa gatgtttttg gttcattcct caatgaactc tcaagaacga
     4801 atcatatcga gataaatggg aaaataaatt ctttcagtgt agatgacata cagatttccc
     4861 ctgaaatcat cacagcttat ttgacagcaa agggagaaat cggcgttcga cttctccctt
     4921 cttcatagca aaatttggaa tattccgtaa aagtttgaat tttttatttt acaatgcccg
     4981 tgaatcccat aaaggcaagt gaaagcagtc ctgcagtaac aagtgctatt ggtgtgcctc
     5041 tcattccttt gggaacctgg ttgagttcta tttgttcacg taaacccgag aagatgatta
     5101 aagccagtcc gaatcctagg gcggtagcaa ttgtataaac cacactttcc atgaggttga
     5161 aattcttctg gataaccaaa atggccacac ctaatactgc acaattagta gtgattaaag
     5221 gaagaaatat tcctaaagcc tgatagaggg aagggcttat tttcttgagg ataatttcta
     5281 ccatttgaac taaggctgca atgacgagaa tatatgctat ggtttgaagg aaagtgattt
     5341 gcagagggat gaggatataa tggtgcacca gaaatgtaac aatagtagcc aatgtcataa
     5401 cgaaaacgac tgctccgctc ataccaatgg ccgtagatag tttattggat acacccagaa
     5461 aagggcaaat gcctaaaaat tgcgttaata caacattatt aactatgatg gcacttacaa
     5521 tgataaggat atattccata gtattaaatc ttaagatgtt ttacgaaatc ggttaatgaa
     5581 agcgataaga aaaccgaggg taataaaagc acccggtgcg agtacgaaaa taagtatacc
     5641 attggaggtg gggttaatga atttatatcc aaaaacagag ccattcccca aggcttcgcg
     5701 gatgcagccc aacataataa gggcaaaagt aaatccaagt cccataccca atccgtcgat
     5761 gagtgaagaa attaccgaat tacgcgaggc aaaagcttct gcacgtccta aaacgataca
     5821 gttcactact atcaacggaa taaatattcc gagtgttttg tgtaaggcag gtacataagc
     5881 cgccatggcc aaatccacca tggttacaaa tgaagctatt accacaataa aagctgggat
     5941 acgtacttta tcggggataa aatccttgag ggccgaaata aataaattcg acatggctaa
     6001 cacaaaagtc gtagccaagc ccatccccag tccattaatg gccgcagaac taaccccaag
     6061 tgtagggcac attcctaaga tgagcgcaaa agtcgggctt tctttaataa aacccttggt
     6121 aaaattactc cattggttca tttttcatct cctctctttt tagcttgata agcgcgttgt
     6181 acagcatcac aaaaagcacg cgaactgatg gtagccgcag taatggcttc tatcgttccc
     6241 tgatctttct tcacttttgg ctgctcattg ggcagtttgg aaatatctaa acctttgaat
     6301 tgatccttga atgcgggttc cgtcattttt gttcccaagc ctggagtttc tttttgctcg
     6361 agaacctcta tgctgtttat cgtaccatcg gccttgaaac ctaccataag gccaatatgg
     6421 cccgagaaac ccctttcgga ataggtttcg acggctacgc ctactaatga atcattctta
     6481 tatccataat aaaggataac agaatctttc ccatcaggag gcaagtaagc tatacgtttt
     6541 aatctatcga aatcagggat gacctgatga atggcatcga tttttttttg gttggcagcg
     6601 gctgcaatag gctccttggt catattgtaa actccggcca aagctgcgct cgcaacaact
     6661 gttatggtca aaaggcaaat taccatattc ttaagagtcg attctttttt tgccatttta
     6721 ttttacctcc ccaaaacgtt taggtttaaa agctctgtcg attaaaggaa caaaagcatt
     6781 catgattaga atagcgaatg atactccctc gggataggca ccaaaaactc gtatcaagat
     6841 ggtaaacact ccacaaccaa ttccgaagat gattttaccc catgctgtca taggcgaagt
     6901 aaccaaatcg gtggccataa aaattgctcc aattaataaa ccaccggcta atatatgaaa
     6961 tacaggatca gcatatcgat ttggatttac taagcataga attccagaaa agataaatgc
     7021 tgtcagtata aaacttactg gaatatgcca agtaatgatt ttgcggtaga gcatataaat
     7081 tcctccaata atcagtgcaa tggccgatac ttcgccaaac gatcctccaa tgttacccca
     7141 taataaattc gtataagaag gaagtttgat ttgagctact gtttgcccca tagccaattg
     7201 ggtctttaaa gttcctaaag gtgtagggcc agtaactcca tcgaaattaa atacattggc
     7261 tcggctttcg atgggcaatg gccaagtagt catggttacc ggaaatgaaa tcagtaaaaa
     7321 cacccttgcg atcaaggcgg gattgaataa atttttaccc aagccgccaa aggccatctt
     7381 tacaattcca atagctacta aactacctat tacgacctgc caccaaggta atgagctggg
     7441 aacattcatg gctaaaagca ggcctgtaac aacggctgaa ccatcattaa cggtaggttc
     7501 ctgtttcaag acaaatttct gtataagaaa ttcgaacaat acacatgagg ctattgatac
     7561 cagggttaca atgactgcac caattccaaa gaaccatacg cctgccagcc atgcgggaag
     7621 aagtgcaatt actactcccc acatgatgga ttttatcgtc atttcaccat gtatatgtgg
     7681 tgaaccagat acaatcaatg gattcatatt gttattgatt tcgagatcga ataatttgat
     7741 tgactttcaa ttttcccaat cggatatggt cgagaagtgg tcgggctgag ggacagatat
     7801 actgacatga gccacattca atacaatcaa gtatagctcc attttcagcc aagtcgaatt
     7861 tttctacttc tgaaactctt gccagataat aaggttcgag ccccataggg caggcactta
     7921 cacactgagc gcagcggata caaggctgaa cctttggtcg taaagactct ttaccgttca
     7981 acacaagaat acccgaagtt cccttaacaa tgggcacgtc tgcatttaat aaggctttac
     8041 ccatcatagg cccacctgct attacctttc cggccttttc aggctgccaa ccagcctttt
     8101 caagcaatgc cgaaacggga gtccctattt tcaccaaaaa attagatgga tgaagcacat
     8161 tggttcctgt aaccgtaacc acgcgttcca ctaaaggcct gtttttttga acggcttgat
     8221 aaacggcaaa ggcagttcca acgttttgaa ctatgcagcc tacttctatg ggaagctttc
     8281 ccgaaggaac ttctcttccc gtcaaggcct gtatgagttg tttttcacct ccctgaggat
     8341 acttgaccct taatgggcaa acttcgatgc cggggtaaga gctggcatgt ttcgaaagaa
     8401 ggttgatggc gtcgggttta ttattttcta tacctattat agctctggta acacccaacg
     8461 ctttcattag aatgcgtgtt ccagtaatga tctcacgagg tttttctagc atgagtcgat
     8521 ggtcagaggt aagatatggt tcacactcca ccccgttgat gatcaataat tcggcctttt
     8581 taccctgagg tatcattaat tttacatgtg tgggaaatgt ggcaccaccc aatcccacaa
     8641 cacccatttc tttgaccttt tgaatgattg aggccccctc aagggttatg tcttctttta
     8701 attcatcact caaattcaca cttgcattcc attcgcttcc ggtaacctct ataaaaatgg
     8761 ctggcttgcg gtatcccgaa acatcgatga aatcatcgat gttttcaatt ttgcccgaaa
     8821 cgggggaatg aagataagaa gaaataaatg attctcctgt agccaaaata tcacctgcct
     8881 ttacttcatc ccctttcttt acgacgggtt ttgaaggagc tcccaagctc tggctcaatg
     8941 gaacgaaaat tttttggggg ggatcaatgg gctgaatggg actatttgcc gaaaatttgt
     9001 tttcgtgtgg atgtacacca ccttttttga atgtcttcaa catctgatct cctattttag
     9061 aggattgaaa atttatatca tgctgtaatt tctgcgggtg aatcggattg agtagtacgt
     9121 ttacgcgggg gaaaattcac ctcgagtata cagttagtag gacaaacttc gacacatttc
     9181 ctgcaaaggg tacatttatg aggatcgata taggctagat tattgctgac agtgatggca
     9241 tcgaagcggc attccttgac gcatttgttg catccaatgc aagctgcttc acaattttta
     9301 cgagcaatgg cacctttttc ctgattcatg caagttataa aaatccgtct gtcttttttc
     9361 ccttttggcc tcatttcgat gatattcctc gggcaggctt tcacacatgc gccacaggct
     9421 gtgcacttat cgtttacttc gggaagtccg gttttaggat tgatatgaat agcatcgaaa
     9481 ctacacgctc tttcacaatc cccttgacct aagcaaccaa aggggcagcc tccttcaccg
     9541 gtaaaagtaa tatgggcaaa ggcacaactc ttaactccat cataatgaaa tttggaagga
     9601 ctgttttgaa aactgccgtt gcatcgtaca acagcaatca tgggttgcat ggttggagcc
     9661 tctaacccta agatgcttgc aattcttttc atgacttcat cgccccccac agggcaaaac
     9721 aaattatcca aagaacctgt ttttacaata gcttcggcaa aattcctgca acccgcatag
     9781 ccacaacctc cacaatttgc ttgcggtaag gcctctgcta ccgtatcgat gcgagggtcc
     9841 tcttctacct tgaatttact ggctacccaa taaagtatag ctccagagcc agctcctata
     9901 actgctaacg taattactgc atataaaatc gatggattca cagctttgag atttttcgac
     9961 cttagttaga tttctgaatt ttcaccacaa aagtataaaa ccctgaagat tcattgtttt
    10021 aagatgtctt ctactaaaaa atattatctt tatagtaaat gactgattat caatataaaa
    10081 tttcctgaac accgattgtt tacatattag cctaagtcct gatttacttt attttaacaa
    10141 actttcttta aattctttct aaattacaga tatttcttaa gtattcctca aaatgcagga
    10201 aagaaaacat gaaatgttgt aaaataattc attttattcc aatggatgaa ctttaaacga
    10261 aaattttttg tgcatccagt ttcttagtat aaataaaacc aaataatagg gaatgaggct
    10321 taaaatagaa cccagacctg cgtatagttc attgtgggtc aggaaataaa ggagggagaa
    10381 tgcaaccact aaaacgatga atggtatcac atagccccat aatactgctg tagaaccttc
    10441 tgattctttt atttcgacaa cgactctctg cccttgatga tattcttgga aatcgggaac
    10501 cgatacatcg atgattttct cggtcatttc acttatactg caaactcctt ttgcatgaca
    10561 ggctgagcaa gccgattgcg aaataatagt tacctgaatc ccttcagggg ctactcgagt
    10621 tacaatccct tcatggctta tggttgatga catataaatt ttaattatgc aaaattagtc
    10681 ctatatttat ctcctttctc ttagttttat aggaagttcg acgaattagg tataaaaatt
    10741 gaataaaaag ccatcctctt cctaaaaaat tcaaaaaggc aaagcatgtg gaaggttcga
    10801 aagagatcga aatttcgaac gaagccattt tgcgggaagg gatagaaaag atattttaat
    10861 gcatcgaaag atgcgagttt aaagactgaa ccggtaaaaa taattacggc aatattggaa
    10921 gtgggtatga gcatttacat tatctctcaa ctcattttca cccttttgcc aaatctccga
    10981 atttttaaaa cccgaagtta tttccaccaa taaggaagaa gatttagcca tgaaggttct
    11041 gaatgaagaa ttcaagaaag aga
//
LOCUS       DAOORU010000071        10880 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7545_length_10880_cov_5.508545, whole genome shotgun sequence.
ACCESSION   DAOORU010000071 DAOORU010000000
VERSION     DAOORU010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7545_length_10880_cov_5.508545"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PLF20_05835"
     CDS             <1..415
                     /locus_tag="PLF20_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80559.1"
                     /translation="KGLKPVGSLEELQKYEEDAPMIITSKAAESESFITPHKVAHGEA
                     IVDLHIEAIAEDYKTLLPDQILDIQLAYFTRCMETAILDKYFKVTFIHGVGNGVLKNA
                     LREKLKDYSNVYFQPAPFARFGMGAIEVMIMHTRE"
     gene            469..866
                     /gene="rnpB"
                     /locus_tag="PLF20_05840"
     ncRNA           469..866
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLF20_05840"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            909..1241
                     /locus_tag="PLF20_05845"
     CDS             909..1241
                     /locus_tag="PLF20_05845"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOX80560.1"
                     /translation="MRFFLKWIASAMAIAIIGWILPGIKVESFLTAFIVAGIIAFLNL
                     VLKPILLFLSIPITIFTMGLFILVINAIIVIIASLLVPGFIVNSFGWALLFGLLNSII
                     HFFLDNAD"
     gene            1251..1712
                     /locus_tag="PLF20_05850"
     CDS             1251..1712
                     /locus_tag="PLF20_05850"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOX80561.1"
                     /translation="MKHRNITDLEEINQILQAADCCYMGMANVDGSPYVVPMNFGFDN
                     QYIYLHSARTGKKIDILRTNPRVCLAFSAFHQLRYQSEKVACSWSMKYKSVLVFGEVE
                     FIEQKEEKVKMLNCLMKKYAGREFTYNVPALKEVLTYKVSLKKVEARAYRY"
     gene            complement(1770..1854)
                     /locus_tag="PLF20_05855"
     tRNA            complement(1770..1854)
                     /locus_tag="PLF20_05855"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1818..1820),aa:Leu,seq:taa)
     gene            complement(1898..1970)
                     /locus_tag="PLF20_05860"
     tRNA            complement(1898..1970)
                     /locus_tag="PLF20_05860"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1935..1937),aa:Gly,seq:gcc)
     gene            complement(2026..4614)
                     /gene="mutS"
                     /locus_tag="PLF20_05865"
     CDS             complement(2026..4614)
                     /gene="mutS"
                     /locus_tag="PLF20_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOX80562.1"
                     /translation="MRQYNRIKAKYPDALLLFRVGDFYETFGEDAIKASQILGIVLTK
                     RSNGAAAMVDLAGFPYHALDNYLPKLVKAGYRVAICDQLEDPKLAKTIVKRGITELVT
                     PGVSTDDRILDQKENNFLASIQLGENLSGISFLDISTGEFLVAQGNNEYIDKLLQNFK
                     PSEIILQKNKLHRFIEIFGDKYYINTFDDWVFTEDFARETLLRHFQVVSLKGFGIEIF
                     PLAIIAAGAAMHYLAETQHDRLSHITRIARIEEEKYVWLDRFTIRNLEIVQTFNENAR
                     SLLQILDQTISPMGARLLRRWITMPIKERKVLEERLDLVELLISDHELAEFLATHLKT
                     VGDLERLISKVSIGKANPREVLYIARGLEAIERIASRCLLTHHPVLMQLCEQLNPCKL
                     IRDRIFKTLKEDCPPVLIKGNIIAERVDEELDHLREILYQGKDVLAKIQQREIQTTGI
                     SSLKIGFNNIFGYYIEVTNAHKNKVPSTWHRKQTLVNAERYITEELKEYEQKILSAEE
                     KIVELENRIFNELLSSVAEYVEPLQLNAGLLARLDVFLSFAQLSASSNYTRPVLNDGL
                     NIDIRAGRHPVIEKQLPAGEQYIPNDVVLNPNNQQIIILTGPNMAGKSAFLRQTALIV
                     LMAQIGCFVPAQKATIGIVDKIFTRVGASDNISLGESTFMVEMNETASILNNISDRSL
                     IILDEIGRGTSTYDGISIAWSIVEYLHNHPLFKPKILFATHYHELNEMARIFPRIKNY
                     HIAVREVGNQVVFLRKLMPGGSEHSFGIHVARMAGMPLKVIERATELLETLENSRNSN
                     SLASSAEKSSKQSSLEGVQLSFFQLDDPLLLKLKDEIHRIDINTITPVEALMKLHQLK
                     KLLGEK"
     gene            4766..5293
                     /locus_tag="PLF20_05870"
     CDS             4766..5293
                     /locus_tag="PLF20_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009121979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOX80563.1"
                     /translation="MIKKTTSELNRISPSEFKRSANDRITVVLDNIRSKQNIGSIFRI
                     CDAFGIGKIFLCGISACPPDREIERTALGATLSVRWKYFSSTSSCIHQLKECGHQIIS
                     IEQTHNSLLLNEFVFPQDKIALVFGNEVEGINEEVLKISHFCIEIPQRGTKHSLNVAV
                     SAGIVLWEVIHKGLK"
     gene            5479..7965
                     /locus_tag="PLF20_05875"
     CDS             5479..7965
                     /locus_tag="PLF20_05875"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOX80564.1"
                     /translation="MKKMYRFIQKAAILAFIGILPIALLGQKENKKSSYTPLEAPHWY
                     VGVQGGISQFYGDITKYRILPDKDNWNFGFGGLLGRQFTPFFGLRGTLAYGNYSGQED
                     RASHSYLPSYDLKVEGSYIDYSLQMTFDLDRLIFGYNPDRKFGVYGLAGIGNSHYRVR
                     QYRISTGELIGSRGVNTESGGGDGKGIDNRDFIGMIPAGLGINYSVSERFDLTLESVL
                     RFTDSEGMDMISGGAKEIKNDFYNTTTLGLVYKFGPSTNLDKMQKEFGKITFETTPAV
                     LETHGDSIDVTIRGTFPPNYFNKKAAICFQPVLKYATGETELKCMVLRGENVQGDAPV
                     ISYKNGGTFTYKTTIPYSPEMNVSELVVSPVAFIAEGPVGKSTKCEDCAKAKKSLVLG
                     ERKLADGVIHTCKRIAATPKQLIAPHGYVRDITVTREAKIYYPKNLYSVNFNFGLNKE
                     EASKKVWANLYDHIKAGYKVKDITINGWASPEGEETFNENLSGNRAQAAKNVLNTDFD
                     KMRKDKKNPVAVPTDLKITTQGNGPDWDGFMKLVEASDLKEKGTILNIVNMADPKKKE
                     QEIRNMILIYPQLEKDLLPPLRRAEIVITAFEPSKTDAEILSSATTEPEKLTEKELLY
                     AATLTQDLKTQQKIYRSATQLHPNSWIAFNNAGVVEIKLGNINEAGADIEKANSLAPD
                     QAEVINNLGALAMLKGDNAKAKQMFEKAKKLGANENYNLGVLMIPEGKYSEAVASMKG
                     EKCDYNLALALMLSGNNGEAVKALECAEKNAQVYYLTAVLGARTGNITLMLDNLGMAI
                     KADGKMKQEAKNDREFLKFANTPEFKKLVE"
     gene            complement(8111..8764)
                     /locus_tag="PLF20_05880"
     CDS             complement(8111..8764)
                     /locus_tag="PLF20_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HOX80565.1"
                     /translation="MATTMKTLVYNYITIEGCIGAGKTSLTQSLADEYNAKLILEQFE
                     ENSFLPKFYSDPLRYAFPLELSFLADRFQQMKVHAVTLDVFHPYIISDYHIQKSLIFA
                     RKTLQPDEFQLFSKLFHLMVSQLPSPELMVYLYLDIQHLMLNIRKRGRQYEQRITTDY
                     LRTIQESYFEFMKQQKNLRILIIDTNRLDFVAKKDDYEMIRDLINNNYPIGITRIQP"
     gene            complement(8800..9303)
                     /gene="folK"
                     /locus_tag="PLF20_05885"
     CDS             complement(8800..9303)
                     /gene="folK"
                     /locus_tag="PLF20_05885"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOX80566.1"
                     /translation="MEQVVLSLGSNQGDRMAYLKKAISFLEYDTKFPLLASSVYQTSA
                     WGFQSNGLFLNCVISLHTNLPAEKILERHQKIEWMLGRERKGEGYSSRSIDIDILFYG
                     DKIIENEGLVVPHPYIEKRKFVLVPLDEILPQFIHPKLQMPVHKLLELCIDKGKVEFF
                     APSVDLF"
     gene            9395..>10880
                     /gene="sppA"
                     /locus_tag="PLF20_05890"
     CDS             9395..>10880
                     /gene="sppA"
                     /locus_tag="PLF20_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOX80567.1"
                     /translation="MKPFWKFFFASVLGTFVTLFIIFLIFFAYIMSSISAATTKPSIE
                     VKDNTVLKFKIDRDILDRKPTNLFAFFNWTEMEPVVPYGLNELLVNLKKASQDPKIKG
                     ILLEISTAPSGMAITEELRIALNEFRSSGKFVVSYGNVYPQKSYYLASLADQIWLNPE
                     GSIELKGLMVDAVFLKKMLEKLDIEAQIIRHGKYKSAVEPFMLDKMSPENREQTFQYV
                     STIWKNIASEIAEGRNLSVEKLDKITDSLLAINPQKALELGIVDRLVYKDEIEDLLLS
                     QQNIDTTSKINYLDFKDYFSAPEPVKKTIDRSKRIAVIYALGDVIDAKGDEQSIGTKN
                     IPEAIQKAREDSKVKAVVLRVNSPGGSALTADLIWREIELTKVIKPVVASFGDVAASG
                     GYYIACNATKIIASPNTITGSIGVFGVIPNAQKFFNNRLGLTFDAVKTNENSDFAGIN
                     RPLTAYQKQVILDEIEEIYSTFVSHVSDGRGMNEVTVDSLGQGRV"
BASE COUNT         3323 a         2081 c         2259 g         3217 t
ORIGIN      
        1 caaagggctc aagccggtgg ggagtttaga agaattgcaa aagtatgaag aggatgcacc
       61 tatgataata accagtaagg ctgctgaatc ggagtcgttt ataacccctc ataaggtagc
      121 tcatggcgag gcaattgttg atctacacat cgaagccatt gccgaagatt ataaaacttt
      181 actacccgat caaatactcg acatacaact ggcttatttt acccgttgta tggaaactgc
      241 cattttggat aaatatttta aggtgacttt tattcatggc gtagggaatg gggtattgaa
      301 aaatgcctta agggagaagc tgaaggatta ttccaatgta tattttcagc ctgctccatt
      361 tgcacgtttt ggaatggggg ccatagaagt aatgattatg catactcggg aatagttttt
      421 gattaagtag ataaaatcat aattatcttt gttctggcaa attgaatgat ggcagtccat
      481 tctatcgcct tggatttcac catccaagga ggaaagtcgg gacaacacag agtagcctgc
      541 ttcctaacag gaaggtatcg catagagcga taacagatag tgccacagaa aagaaccgtc
      601 gaagggttgg ttcagccaac aatcgacaag ggtgaaaacg tgaggtaaga gctcacgctg
      661 caggatggcg acatcttgtg ggggtaaacc tcaggcgttg aaaggccaaa taaaccaggg
      721 atgctctatg gttcatagag tgagagggtt actcgcccaa tcctggaggg taggccgata
      781 gaggtaacag gtgactgtta tcccagataa atgatagaaa tcctgaattg aggatacaga
      841 atcccgctta tagggctgcc atcttttcat ttgcctttat tgattagaaa acctaactaa
      901 tttgcaatat gcggtttttc cttaaatgga tagcctctgc aatggccata gccattattg
      961 gttggatact cccgggtata aaagtagaaa gttttttaac tgcttttatt gtggctggca
     1021 tcattgcctt tctgaatctt gttcttaaac ctatcctact gtttcttagt attcctatta
     1081 ctatttttac catgggatta tttattcttg taatcaatgc tattatagtg ataattgcat
     1141 ctttacttgt tcctggcttt atcgttaata gctttgggtg ggcgctgctc ttcggccttt
     1201 taaattccat cattcatttt tttcttgata atgctgatta aattcaaaat atgaaacatc
     1261 gtaatattac cgatctggag gagatcaacc agatacttca ggctgccgat tgctgttata
     1321 tgggaatggc gaacgtggat ggaagtcctt atgtggttcc catgaatttt ggattcgata
     1381 accagtatat ttacttgcat tcagcaagaa ctggtaaaaa aatcgatatt ttacgaacca
     1441 atcccagagt ttgtctggct tttagcgcat ttcaccaatt gaggtatcag agcgaaaaag
     1501 tagcctgttc atggagtatg aagtataaaa gtgttttggt tttcggtgaa gtggaattta
     1561 tcgagcaaaa agaagaaaaa gtgaagatgt tgaattgctt gatgaagaaa tatgctggca
     1621 gggaatttac ttataatgtt cctgccttga aagaagtact cacttacaaa gtttcattga
     1681 agaaagtcga agctcgggct tacaggtatt agaaacaact catcacaaaa aacctggtta
     1741 acatcatgca aggttaaggc ttttaccagt acccggggcg ggacttgaac ccgcacgacc
     1801 tcaacggtca aaggatttta agtccttcgt gtctacctct tccaccaccc gggccgatag
     1861 gaaaacccga atctgcctgg gcagcacccg ggctttgagc ggaaaacggg actcggaccc
     1921 gcgaccccaa ccttggcaag gttgtgctct accagctgag ctatttccgc ttttcaattt
     1981 tatgtctgca aaattaatag tattttctgt ttccttccaa attctttatt tctctcccaa
     2041 taattttttt agttgatgca acttcatcaa ggcttctact ggagttattg tattgatatc
     2101 gatgcgatga atttcgtcct ttaattttaa caatagagga tcatccaatt gaaagaaact
     2161 caattgaaca ccttcgagtg aggattgttt cgacgatttt tcggctgaag aagccaaaga
     2221 attactgtta cgcgaatttt cgagcgtttc aagcagttct gtagcccttt cgattacttt
     2281 taaaggcata cctgccatac gtgccacatg tattccgaaa ctatgttcac ttcctccagg
     2341 catcagtttt ctcagaaaaa caacctgatt cccaacttcc cttactgcaa tgtgataatt
     2401 ctttatacgg ggaaatattc tggccatttc gttcagttcg tggtaatgtg tggcaaagag
     2461 aattttcggt ttgaataaag gatgattgtg gagatattct actatgctcc atgcaataga
     2521 aataccatca taagtactgg ttcctcgtcc aatttcatcg agaatgatca agctacggtc
     2581 ggaaatgttg ttcaaaatgc ttgctgtttc gttcatttcg accataaagg tagattcacc
     2641 aagggaaata ttatcggagg ctccgacgcg cgtaaaaatc ttatcgacaa ttcctatggt
     2701 agctttttgg gcagggacaa aacatccaat ctgagccatc aatacgatga gagcggtttg
     2761 gcgaagaaag gccgattttc ctgccatgtt aggaccggta agaataataa tctgctgatt
     2821 atttggattc agtactacat cattagggat atactgttct cctgcaggaa gttgcttttc
     2881 gatcactgga tggcggcctg cgcggatatc gatgttcaat ccatcattta gcacaggtcg
     2941 ggtataattt gaacttgccg aaagttgtgc aaacgacagg aaaacatcta aacgcgctag
     3001 taatccagca ttcaattgca aaggttccac atattcggct accgagctaa gtaactcatt
     3061 aaaaattctg ttttcgagtt ctacaatttt ttcttcggcg ctgagaatct tttgttcgta
     3121 ttcctttagt tcttcggtaa tatatctttc ggcgttcact aatgtttgct tgcgatgcca
     3181 agtggatggt actttatttt tatgtgcatt ggttacttct atataatagc caaagatatt
     3241 attgaatcca attttgaggg atgaaattcc ggtagtttgg atctctcttt gctggatttt
     3301 agcaagcaca tctttgccct gatagagaat ttcacgtaaa tggtccaatt cttcatcgac
     3361 tctttcggca atgatatttc ccttgatcaa tactggtggg caatcttcct tgagtgtttt
     3421 aaatatgcga tctctgatga gtttgcaggg gttcagttgt tcgcataatt gcatcaatac
     3481 aggatgatgt gtaagaagac agcgcgaagc aattcgttcg atggcttcga gcccccgggc
     3541 aatataaaga acttcacgtg gattggcttt tcctatgctt acttttgaaa tcaggcgttc
     3601 caaatcgcca acagttttga gatgggtagc cagaaattcg gccagttcat ggtcagaaat
     3661 aagaagttct accaggtcga gacgttcttc aagtacttta cgttccttta taggcatggt
     3721 gatccaccgc cggagcaggc gggctcccat cggactgatg gtttgatcga gaatttgaag
     3781 aagtgagcgg gcattttcgt tgaaggtctg aacgatttcg agatttcgaa tcgtaaatct
     3841 gtccagccaa acgtattttt cttcttcgat acgggcaata cgagtgatgt ggctcaaacg
     3901 atcatgctga gtttcggcaa gataatgcat agcagctcca gcggctatga tagctaacgg
     3961 aaatatttct atcccgaatc ctttgaggga aacaacctga aaatgcctca gcaaagtttc
     4021 acgcgcaaaa tcttcggtaa atacccaatc atcaaaggta tttatgtaat atttatctcc
     4081 gaaaatctcg ataaaccgat gcaatttatt cttttgaaga atgatttcgc tgggtttgaa
     4141 attttgcaga agcttgtcga tatattcatt attaccctga gctacgagga attcacctgt
     4201 ggagatatcc aaaaaactta tgcccgaaag gttttcgccc agctgaatgc tggcaagaaa
     4261 attattttcc ttctgatcga gaattctatc atcggtgctc acaccaggag taaccaattc
     4321 agtaattcct cttttgacta tggtttttgc cagtttgggg tcttccagtt gatcgcaaat
     4381 agccactcta tagccagcct tgaccagttt tggtaagtaa ttatcgagag catgataggg
     4441 aaatcctgct aaatcaacca tggcagcggc accattcgat cttttagtaa ggacaatgcc
     4501 aaggatttga gaggctttta tggcatcttc gccaaaggtt tcataaaaat cacccactct
     4561 gaataaaagt agtgcgtcag gatattttgc cttaatcctg ttatattgcc gcattaatgg
     4621 cgtatctgac gaattggatt tcaagagaaa aataattttc ggtttcagca aaattaatgt
     4681 gaatctgaca agccggcaat ctaatctatc tgtcaatttt gcagtttaca ctgccagtgc
     4741 ctatttttgc aaaaaacatc gcactttgat aaagaaaaca acttcagagt taaatagaat
     4801 ttcaccttct gaattcaaaa gaagtgccaa tgacagaata actgttgtac ttgacaatat
     4861 cagaagtaag caaaacatag gctctatatt tcgaatttgc gatgcatttg gcattggtaa
     4921 aatttttctt tgtgggatat cggcctgtcc gcccgacagg gaaattgagc gaactgcttt
     4981 aggagccaca ctttcggtga ggtggaaata tttttcgagt acgtccagtt gtattcatca
     5041 gctcaaggaa tgtggtcatc aaattatcag tatcgaacaa actcataaca gccttttact
     5101 caacgaattc gtatttcctc aagacaagat agcattggtt tttgggaacg aggtagaagg
     5161 tattaacgaa gaagtattga aaatttctca tttttgcatc gagattcctc aaagagggac
     5221 caaacattct ttaaatgtag cggtaagtgc cggaattgtc ttatgggagg tcatccacaa
     5281 aggtttaaaa taaggcgttt ggcataattt ttaggagaaa aactcttaaa atgaaacctt
     5341 tttttgagtt tatcagttat aaaataaaaa gattgatgat tttgaaagaa atgaacttca
     5401 aattttttat aaatttgcct acttttaata aaaatattat ttttgtaaaa tatcgtaaac
     5461 cataaacctt accaaactat gaaaaagatg tatagattca tccaaaaagc tgcaattctg
     5521 gcctttatag gcatattacc catagcctta ttagggcaga aagagaataa gaaaagcagt
     5581 tatactccgt tggaggctcc tcattggtac gtaggtgttc aaggtggtat aagccaattt
     5641 tatggtgata taacaaagta caggatatta ccagacaaag acaattggaa ctttggattt
     5701 ggaggattgc ttggccgcca atttacacca ttcttcgggc ttagaggtac acttgcctat
     5761 ggaaattata gtgggcagga agaccgtgcc agtcatagct atttaccctc ttacgaccta
     5821 aaggttgaag ggagttatat agattattct cttcaaatga catttgatct cgatcgcctt
     5881 atttttgggt ataaccctga tcgtaagttt ggagtttatg ggctggcagg tatcggaaat
     5941 tcgcattata gggttagaca gtacagaatt tcaacgggag aattaattgg aagtcgtgga
     6001 gtaaacactg aaagtggtgg aggcgatggg aaaggcatcg ataaccgtga ttttattgga
     6061 atgatacctg cgggtttagg aattaattat tccgtttccg aaagattcga tcttaccctc
     6121 gaaagtgtgc ttcgctttac cgacagtgaa ggaatggata tgatctcggg cggagctaaa
     6181 gaaatcaaaa acgatttcta taacacaact actttaggcc tcgtatacaa atttgggcca
     6241 agtactaacc tggataaaat gcaaaaagaa ttcgggaaaa ttacttttga aaccacccct
     6301 gcagtgctcg aaacacatgg cgattctatt gatgttacta ttcggggcac attcccccct
     6361 aactatttca acaaaaaagc tgccatatgt tttcagcccg tattgaaata tgccacgggt
     6421 gaaaccgaat tgaagtgcat ggttttgaga ggagaaaatg ttcagggtga tgctcccgtt
     6481 atttcttaca aaaatggtgg cacttttacc tataaaacaa ctattcctta ctctcccgaa
     6541 atgaatgttt cggaattggt cgttagcccg gtagcattta ttgctgaagg tccagttggt
     6601 aaaagtacca aatgtgaaga ttgtgccaag gctaagaaat cattggtact gggcgagcgt
     6661 aaattggccg atggcgttat tcatacctgc aaacgcatcg ctgccacgcc taaacaactt
     6721 atcgctcccc atggttacgt aagagatatt acggttacgc gtgaagctaa aatatactat
     6781 ccaaagaatt tatattcggt aaacttcaac tttggattga ataaggaaga ggcttcgaaa
     6841 aaggtatggg ctaatttata tgatcacatc aaagcaggtt acaaggtcaa agatattacc
     6901 atcaacggat gggcatcacc cgaaggtgaa gaaaccttca acgaaaacct ttcgggcaat
     6961 cgtgcccagg cggctaaaaa tgtactgaat accgattttg ataagatgag aaaagataag
     7021 aaaaatcctg ttgccgtacc taccgactta aaaattacta cgcaagggaa tggtccggat
     7081 tgggatggat tcatgaaact agttgaagcc agcgacctga aagaaaaggg aacaatcctg
     7141 aatatcgtta atatggccga tccaaagaaa aaagaacagg aaatccgcaa tatgatcctg
     7201 atttatcctc aactcgaaaa agatcttttg cctcctttga gaagagccga aatcgtaatt
     7261 acagcattcg aaccttcgaa gacagatgcc gaaatcctga gctcggctac tactgaaccc
     7321 gaaaaactta ccgaaaagga acttctttat gctgctacac tcactcaaga ccttaaaacc
     7381 cagcaaaaga tatatcgttc ggctacacaa ttgcatccca atagctggat agccttcaac
     7441 aatgctgggg tagtcgaaat taaactcggt aacatcaatg aagccggagc agacattgaa
     7501 aaggccaata gcttagctcc tgatcaggct gaagttatca acaatttagg ggcattagcc
     7561 atgctgaagg gcgataatgc aaaagccaaa cagatgttcg aaaaagcgaa aaaattagga
     7621 gccaatgaaa actataacct tggtgtgttg atgattcccg aagggaaata cagtgaagcc
     7681 gtggcttcga tgaaaggtga aaagtgcgat tacaatcttg cactcgccct tatgctttcg
     7741 ggaaataatg gtgaagcagt gaaagcttta gaatgtgccg aaaagaatgc tcaggtttat
     7801 tatcttactg ctgttttagg tgctcgcaca ggtaatatta ccttaatgct cgataatttg
     7861 ggaatggcta ttaaggctga tggtaaaatg aaacaagaag ccaagaatga tagagaattc
     7921 cttaagtttg ccaatactcc cgaattcaaa aaattagtcg aatagagata ttttaggatg
     7981 aggttttata ttgcgggctc gcctctttga ggcgagcccg tatctttagt atctctctca
     8041 ttcccctcac aataaatcac tcctactaat aaaatgatga tagtatcacc ctgaatgacc
     8101 tcaaaataga ttagggctga atccgtgtaa tccctatggg ataattgttg ttgatcaaat
     8161 ctcttatcat ttcataatca tcctttttgg ccacaaaatc cagacgattt gtatcaatta
     8221 ttaaaatcct caaatttttt tgctgtttca tgaattcaaa ataactctcc tgtatggttc
     8281 gaagataatc cgtagttatt ctctgctcat attgtcgccc acgctttcga atgttcaaca
     8341 ttaaatgctg tatgtcgaga tagagatata ccatcaattc tggtgagggc agctggctaa
     8401 ccattaagtg aaagagtttc gaaaagagct gaaactcatc cggctgcaag gttttccgtg
     8461 caaaaatgag cgatttctga atgtgataat ctgaaatgat ataagggtga aaaacatcca
     8521 gggttacggc atgaaccttc atttgctgaa atctatcggc gagaaatgaa agttcgagag
     8581 gaaaagcata tcgcaaagga tcagaataga attttggcag aaatgaattt tcttcgaact
     8641 gttcaagaat caatttggca ttatactcat cagccagtga ctgggtcaat gaagttttgc
     8701 ctgctcctat acatccttct atggttatat aattataaac caaagttttc attgttgtag
     8761 ccatcactta taaatgaaaa actgaaaatt aagaattttt tagaataaat cgacagacgg
     8821 agcaaaaaat tcaacttttc ccttatcgat gcataattca agcagcttat gaaccggcat
     8881 ctgcaatttt ggatgtatga actggggtaa tatttcatct aaaggtacca acacaaattt
     8941 tcttttttcg atataaggat gaggcacaac caacccttca ttttcgatga ttttatctcc
     9001 ataaaaaaga atatcgatat caatactacg ggatgaatat ccctcccctt ttctttctct
     9061 accaagcatc cattcgattt tctgatgcct ttcgaggatt ttttcggctg gtaagttcgt
     9121 atgaagagaa attacacaat tcaagaaaag cccattggac tgaaaacccc aggctgatgt
     9181 ttggtacact gacgaagcca atagcggaaa tttcgtgtcg tattccaaaa acgaaatggc
     9241 tttctttaaa taggccatcc tatcgccctg attgcttccc agtgataata caacttgttc
     9301 catagatttc aattatgttc ccacaatatt attcatttct acattcacga gaaaatttaa
     9361 ctttgtgcga taaaatctta aaaaattctt acccatgaaa ccattttgga aattcttttt
     9421 tgcttcggtt ttaggcacat tcgtgactct ttttatcatt ttcctgattt ttttcgcata
     9481 catcatgagt tcaatttcag cagccacaac caaaccaagc atagaagtca aggacaatac
     9541 tgttttgaag tttaagatcg atcgagatat ccttgatcgt aaacctacca atcttttcgc
     9601 atttttcaac tggacggaaa tggaacccgt tgtcccttat ggcttgaatg agctactcgt
     9661 caatttgaag aaagcttcac aagatccaaa aataaagggt attttactcg agatttcgac
     9721 agcaccttcg ggtatggcta tcactgaaga gctacgcatt gcgctgaacg aattcagatc
     9781 ttcggggaaa ttcgtcgtaa gttacgggaa tgtttatcca cagaagagct attatttggc
     9841 ctcgctggct gatcaaatct ggttgaatcc tgaaggaagc atagagttaa aaggattaat
     9901 ggtggacgca gtgtttctga aaaaaatgct ggaaaagctt gatatcgaag cacaaatcat
     9961 acgtcacgga aaatacaaga gtgccgtaga accgtttatg ctcgataaaa tgagccctga
    10021 gaacagagaa caaacctttc agtatgtaag taccatctgg aaaaatatcg cctccgaaat
    10081 tgccgagggt cgaaatcttt ctgtagagaa gctcgataaa attaccgaca gtcttttggc
    10141 tataaacccg caaaaagcct tagaacttgg cattgttgac cgtttggtct ataaagatga
    10201 aatcgaagat cttttattgt cgcaacagaa catagatact acttccaaaa tcaattattt
    10261 agactttaaa gattatttca gtgctcctga acccgtaaag aaaaccatag atcgttccaa
    10321 aagaattgct gtaatttatg ccttaggcga tgtaattgat gcaaaaggtg atgagcagag
    10381 tattggtact aaaaacatac ccgaagccat tcagaaggca cgagaagata gtaaggtaaa
    10441 ggcagtggtg ctacgagtga acagtcccgg tggaagtgcc ctcacggccg atctcatctg
    10501 gcgcgaaatt gaactcacga aagtcatcaa acctgttgtg gcttcatttg gcgatgtagc
    10561 tgcctcagga ggatattata ttgcctgcaa tgccactaag atcattgctt cacccaatac
    10621 tattacaggc tcaattggag tttttggcgt gattcccaat gcacagaaat tcttcaataa
    10681 tcgcttgggc ctcactttcg atgcagtaaa aaccaatgaa aattccgatt tcgccggtat
    10741 taaccgcccc ttgactgctt atcaaaaaca agttattctt gatgaaattg aagagattta
    10801 ctcgactttt gtgtctcatg tttccgatgg ccgtggaatg aatgaagtta cagtggatag
    10861 cctcggacaa ggaagagtat
//
LOCUS       DAOORU010000072        10873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7551_length_10873_cov_5.465613, whole genome shotgun sequence.
ACCESSION   DAOORU010000072 DAOORU010000000
VERSION     DAOORU010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10873
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7551_length_10873_cov_5.465613"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="PLF20_05895"
     CDS             complement(<1..1772)
                     /locus_tag="PLF20_05895"
                     /inference="COORDINATES: protein motif:HMM:NF037979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HOX80568.1"
                     /translation="MGKNFKTADLKGEHWGSRVGLVLAMAGNAVGFGNFLRFPVQAMQ
                     NGGGAFIIPYLVCLVLLGFPLLMIEWTVGRYGGKFGHHSTPFILHSMSKKAVWKYVGV
                     FGLFSNLAIAAYYCYIESWTMSYILHSIFGTFDHLSRLQVSSFFDGYVDISHSTTGIP
                     YEAIVFYILCLALNAWILSRGLAGGVEKASKIGVPLLIVFGIFLAIKAITLKAGNENA
                     NYDGLVGLSFLWTPQYDSLTNPKVWLAAAGQIFFTLSVGMGSIQCYASYLRERDDIAV
                     NAMSAGFMNEFVEIVLGSSIIIPISVGYLGIDKVIDLVSLGGLGLAFRTMPFLFEQWG
                     TILSVIAGVAFFGLLFFAGITSSLAMGTPIMGFLKDEFKFNNKKASFTFALVTLALGL
                     PVVLFFQQGVFDEFDYWAGTVALVVFAMFESILFSWFFGADKGWSEFMKGADMKLPNI
                     FRYILKYITPVLLIIVFITSMIRPANDDWTSLSFKGWELHPESILGQITYKGIIYNKQ
                     NVADVFYSENSGIVDSIYSLRGKDYVRITPEGKTAELGKNYIYKEHQKVLVQLGDVVN
                     AGQPIFEGRVINRILYIKFARFILL"
     gene            complement(1977..3239)
                     /locus_tag="PLF20_05900"
     CDS             complement(1977..3239)
                     /locus_tag="PLF20_05900"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOX80569.1"
                     /translation="MTESFVHYLWKNNLLKPELQTTSGQPITIIQPGIHNEDAGPDFI
                     QAIIKIEDEIWAGNVEIHLHTSDWYKHGHEKDKAYQNIILHAVFENDLSGQEPKEFPT
                     LELKPFTDPCLWNRYQTFLMSKDWIPCARNFHEAFPLTTHSMLEISLVMRLERKIEEI
                     SKIVTLNKNNLSEAFYIILTRNFGLKANIQPFELLARSLPLNLLAKHKGNLFQIEALM
                     FGQAGMLAEASDDYQRKLKEEFHFLKNKYHLTPIPVYLWKFLRMRPASFPTIRIAQLA
                     ALINTSSHLLSKLSECNSLIEMEELLACRASNYWDTHYRFGEISAVRPKNAGKDFIHS
                     IIINSVIPFIHIQAVYTGKSGHQKHIENLLSDLPPENNQILRGWETLGYKAKDAFESQ
                     ALIELKTHYCNLKRCLDCRIGHSLLTQK"
     gene            complement(3274..4461)
                     /locus_tag="PLF20_05905"
     CDS             complement(3274..4461)
                     /locus_tag="PLF20_05905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX80570.1"
                     /translation="MDSPDDCDVAIAGAGPAGLACALTLKGRGLKIKIFDKKAFPRSK
                     PCGGELGIRAINTLRKLSFNNKKLLDGFDNKNPIYGFQINTLKHNNYSFLLDLQKNDV
                     QPMGYTVNRQEFDFYLLQNLLDDPFIEFYPQTSVLKTLKNEGNIELITEKNFHKTSIL
                     IDATGVKHIRDKSVRNSWAFAISSFVKDLQIEEHQQNILQFYFTSYIFPGYFWIFPLQ
                     EGLANVGLYVPITYYKASLLQLRHYFFELIQSHSILAGQFQSASLASSLQGGWLPLGY
                     PKGSYSEGRILKVGDAARLVDPLAGEGIGNALLSGHLAGLHILKCFEENNFSDEFLST
                     YNHIIANIFEKEFKSRHRIVKYLSKHPKTFDRCFHQLSNNRDFRKFIFKIFLSSQYSS
                     KLL"
     gene            complement(4656..4973)
                     /locus_tag="PLF20_05910"
     CDS             complement(4656..4973)
                     /locus_tag="PLF20_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HOX80571.1"
                     /translation="MNIFVGSLPFKLQETDLREIFEEHGEVTSVKIITDKFSGRSKGF
                     GFVEMPNDAEAQQAIDELNGAEVDGRTIVVNKAEERKEGPRRNNHRGGNDNRGGYQGN
                     RRY"
     gene            complement(5256..5810)
                     /locus_tag="PLF20_05915"
     CDS             complement(5256..5810)
                     /locus_tag="PLF20_05915"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOX80572.1"
                     /translation="MLEEGLTLQRIEQDKMRNIRNPFVDEEDNHCFGCSPTNPYGLHL
                     KFKETDDAITAEWLFTENYCGYHDILHGGIQACLMDEIASWYVFVKLKTGGVTASMKF
                     RYLQPVSGIGSNIHLEARLIEQKHHLAFIEVTLSKEDTGVCTRAMIIYYLFPEKIARE
                     KFKLPHPTAFYYSKEEAGKEDGVW"
     gene            6215..6961
                     /gene="fabG"
                     /locus_tag="PLF20_05920"
     CDS             6215..6961
                     /gene="fabG"
                     /locus_tag="PLF20_05920"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007652512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOX80573.1"
                     /translation="MKLLEGKTALITGAARGIGRSIALKFASEGAHIAFSDLRLDEAT
                     QILEEEIRGMGVEAMAYASDASSFSDSEILVTQVIEDFGTIEILVNNAGITRDNLLMR
                     MSEADWDLVMKVNLKSVFNLTKAVQKIMLKNRFGSIINMSSVVGVKGNAGQANYSASK
                     AGIIGFTKSMAQELGSRNIRCNAVAPGFIDTEMTAKLSEEVKREWAEKIPLRRAGLPE
                     DVANSCLFLASDLSSYITGQVIHVCGGMVM"
     gene            6980..9058
                     /locus_tag="PLF20_05925"
     CDS             6980..9058
                     /locus_tag="PLF20_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80574.1"
                     /translation="MSFIFENSGWWLVLAPVVGFVYAFVLYRNTHDIFDRKIQILLGI
                     LRFVVVTILVIVLMGVLIKLTSHRFEKPLVVLALDNSQSMAQLPDSTEIRKNLPGLLK
                     NLESKLAPEFEVKKVSFGSSVNDTLNFRFDESETNMASLLTYIQERYPNRIIGGLVLV
                     SDGIINRGQDPLTMAESKLYPIFPIAMGDTSTRRDLFFQEVRYNQITFTGNQFPVEIY
                     LKAESCNSQKSSVTISSNNKKLWTSDFTVSSENQIITFSPALMAEKPGIIRYTIEIKP
                     IEEESNVKNNRMDFFIEVKDTRVKVLILADAPHPDIAALKKVFIFRDIYQAEDYLISN
                     FDKDIHQFDLIIFHQLPSTRVSVNDIYEQALKAGKPVMFILGQSSDIELLNAMEIGLK
                     VTATGKKFVNAYPLLVADFQLFRTDHRLVETFDKLPPLISPDGKYSVSPIAVPYLLQK
                     IDNVLTSNPLILFHRKENQSVAIIAGEGLWWWRNILYQNYRDHEIFEDFIHNMVQYLI
                     SSEPTGNFRVKVGNRFRGNEHVKFNAELYDATFKLVNEPEVHLEIKDENGVRYPFIFN
                     RIGDIYELDAGIFNPGIYSWTARTTFNKEELSAGGIFVVEHTIAEKNYLKANHRLLFD
                     LSENSGGKMVWPVQTDSIPQWLKKMGNTATKVYTEESYVKLNDLGWLLALMIILISGE
                     WAIRKWSGIL"
     gene            9094..10389
                     /locus_tag="PLF20_05930"
     CDS             9094..10389
                     /locus_tag="PLF20_05930"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOX80575.1"
                     /translation="MNTTVIYLFYAFLAILILSFAAERILDWLNIRNWPKVLPDLIRD
                     IFPEEKFMEVRQYQLVNYKIGFIEATITSIITIGFLYFGGFAWLDGVARSFSSNLIVQ
                     SLIFFGIIAVATFIIRLPFDIYDTFVIESRFGFNTTTPRIYITDQLKSALLTILIGVP
                     LFSLIVFLYQKTGEYFWVLVWLVISIFALFMSLFYSSLIVPLFNKQTPIPEGELRTAI
                     EEFAHKAGFKVKDIYVIDSSKRSSKANAYFTGLGPEKRIVLYDTLIKDFSIDEILAVL
                     AHEIGHYKHHHTTIGLITSIVQTGLILFVFSLFVKPGSELTEVLCNAAAGARQVTPSF
                     YMGLIGFTLLFSPISAIIGLIFNSISRRHEFQADAYVAEHQMQGYLQSALKKLSVNHL
                     SNPFPHPLYVKVYYSHPPLIERLKALENTSRREPQGNGF"
     gene            complement(10611..>10873)
                     /locus_tag="PLF20_05935"
     CDS             complement(10611..>10873)
                     /locus_tag="PLF20_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80576.1"
                     /translation="TYGKKNYIRTNDNPKGLLAPSINGNPITEFRIMDFGKPYMEVSY
                     GIENILKIIRVQAFHRLSYLDPGPDGKKIPKFMIKASFFIRL"
BASE COUNT         3273 a         2067 c         2203 g         3330 t
ORIGIN      
        1 aggagaataa agcgggcaaa tttaatgtat agaatgcggt taatgactct gccttcgaaa
       61 atgggctgtc cggcgtttac cacatcaccc agttggacga gcactttttg atgttcttta
      121 tagatgtaat ttttcccaag ttctgcggtt ttcccttcgg gtgtaatccg aacataatct
      181 ttcccacgca atgaatagat agaatcaact ataccggaat tttcggagta aaacacatcg
      241 gccacatttt gtttattgta gataatgcct ttataggtaa tttgcccgag aatgctttca
      301 gggtgaagtt cccagccttt gaagctcagg gatgtccaat cgtcgttagc cggacggatc
      361 atggaagtaa tgaaaacaat gatcagtaat acgggagtaa tatatttcag gatatagcgg
      421 aaaatattag gcagtttcat atcggcacct ttcatgaatt cactccagcc tttatctgcc
      481 ccaaaaaacc atgaaaacag tatggattcg aacatagcaa aaacaaccag agcaacggtg
      541 ccagcccagt agtcgaattc gtcgaaaaca ccttgttgaa agaataacac aacaggtagt
      601 cccaaagcga gtgtaactaa agcaaatgtg aaggatgctt tcttattgtt gaatttgaat
      661 tcgtctttta gaaaacccat tatgggcgtc cccatggcca aagaagaagt tattccagca
      721 aagaaaagca atccaaagaa agccacacca gcgatgacgg aaagaatagt cccccattgt
      781 tcgaaaagga agggcatggt gcgaaaagcc aagcctaaac ctcccagact aaccaggtcg
      841 ataactttat ctatacccaa ataacctacc gaaataggga taatgattga gcttccaaga
      901 acaatctcga caaattcgtt catgaatcct gccgacatgg catttactgc aatatcgtca
      961 cgttctctca agtaagaggc ataacattgt atagaaccca tgcccaccga aagtgtaaag
     1021 aagatttgcc ctgcagcagc cagccagacc ttgggattgg taagagaatc atactgaggg
     1081 gtccacaaga atgagaggcc aactaagcca tcataattgg cgttttcgtt acctgctttt
     1141 aaggtaatag cttttatggc taaaaaaatt ccaaatacaa taagcaaggg aactccaatc
     1201 ttcgaagctt tttctactcc tcccgccaaa ccccgactta aaatccaggc attgagggcc
     1261 aggcacaata tataaaaaac gatagcttca tagggtatac ccgtagtgga atggctgata
     1321 tctacataac catcgaaaaa actacttacc tgaagccggc tcaaatgatc gaaagtacca
     1381 aaaatgctgt gaagaatata cgacatggtc cagctttcta tgtaacaata gtaagccgca
     1441 atggctaaat tgctgaaaag cccaaaaact cccacatatt tccagactgc ctttttgctc
     1501 atgctatgaa ggataaatgg ggtggaatga tgtccgaatt ttccaccata tcttcctact
     1561 gtccattcga tcatcaataa gggaaatcct aataatacaa ggcagacaag gtagggtata
     1621 ataaatgctc ctcctccatt ctgcatggct tgtaccggaa aacgcaaaaa attcccgaac
     1681 cctacagcat ttcctgccat cgctaaaaca agacctactc tcgaacccca atgctcccct
     1741 tttaaatcgg cagtcttaaa atttttcccc atccccgtgg tgctaattta ttaatatata
     1801 tattattatg tttattatcg aagtttttct tttacgaaaa atgacttgtt ttcgaataaa
     1861 attttcgagg ctctaaaata aaaaatattg aaaacataaa ttgatgaacg aaaaatcatt
     1921 cacgcgacag gaaaaccgta tgggaagaaa ttagcctgat ttatccgatg tcatcgttat
     1981 ttttgggtaa gcaaagaatg tccgattctg caatcgaggc aacgtttcag gttgcaataa
     2041 tgcgttttga gttcgataag agcctgactt tcgaatgcgt ctttcgcttt atacccaagc
     2101 gtttcccagc ctcttaaaat ttgattgttt tcgggaggca agtccgataa aagattttcg
     2161 atatgtttct gatggccgct tttgcccgta taaacggcct gaatatgaat gaaaggaatg
     2221 acagagttga taatgataga atggataaaa tcctttccgg cattttttgg tctgacggca
     2281 ctaatttctc caaaacgata atgtgtatcc caataattcg aagcccggca agccaaaagt
     2341 tcttccattt cgatgagaga attgcattct gagagtttcg acaaaagatg tgatgaagta
     2401 ttgatgagcg cggccaattg tgcaatccga atggtaggaa aacttgcagg cctcattctt
     2461 aaaaatttcc acaaatagac gggaatagga gtgagatgat atttgttctt caggaaatga
     2521 aattcttctt ttaatttcct ttggtaatcg tcagaagcct cggcaagcat gcctgcctga
     2581 ccgaacatga gggcttcgat ctgaaaaaga ttgcctttgt gtttggctaa taaattcaat
     2641 ggcaatgacc tggctaaaag ttcaaatggt tgaatattgg cttttaaacc aaagttacgt
     2701 gtcaaaatga tataaaaggc ttctgaaaga ttgtttttat tcagggtcac gatttttgaa
     2761 atctcttcga tcttccgttc gaggcgcata acgagggaaa tttcgagcat ggaatgtgtg
     2821 gtaagtggaa atgcttcgtg aaaatttctt gcacagggga tccagtcttt tgacattaaa
     2881 aaagtttgat aacgattcca taagcatgga tcagtaaagg gtttcagctc gagagtggga
     2941 aattctttgg gttcttgccc actgagatca ttttcgaaaa cagcatgcag aatgatgttt
     3001 tgataggcct tgtccttctc gtgcccatgc ttgtaccaat cagatgtatg caaatggatt
     3061 tcgacgtttc cggcccatat ttcatcttcg atttttatta tggcttgaat aaaatcgggc
     3121 cctgcatctt cattgtgtat tcctggttga ataatggtaa taggttgacc cgatgtggtt
     3181 tgcagttctg gtttcaacag gttgtttttc cataaataat gcacaaagga ttcggtcatt
     3241 ccatttttat attattttga aaaccaaaca aatctataat aatttggaag aatattgcga
     3301 agagaggaag attttgaaaa tgaacttgcg gaagtctcta ttgttgctca actgatgaaa
     3361 acatcgatcg aaagttttgg ggtgcttcga tagatattta acgattctat gtcttgattt
     3421 gaattctttc tcgaaaatat tggcaatgat atgattataa gttgaaagga attcatctga
     3481 aaaattattt tcttcgaaac attttaagat atgaagcccg gccaggtgac cactcaaaag
     3541 tgcattacct attccttctc cggccagggg gtccaccaga cgtgctgcat cgccaacttt
     3601 cagaattcta ccctccgaat aactgccttt ggggtatcca agaggcaacc agccaccctg
     3661 aagtgatgag gctaatgaag cactctgaaa ttgacccgcc agaatgctgt ggctctgaat
     3721 gagctcgaaa aaatagtgtc gcaactgcaa aagtgaggct ttataataag ttattggcac
     3781 atacaaacct acgttggcca atccttcctg cagtggaaaa atccagaaat agcccgggaa
     3841 aatataagac gtaaaataga attgcaggat gttttgctga tgttcttcta tctgaaggtc
     3901 tttcacgaaa gatgagatgg caaaagccca tgagtttctg acagatttgt cacgtatatg
     3961 tttaactcct gtagcatcta tcaaaatgga agtcttgtga aaattctttt cggtaatcag
     4021 ctcgatgtta ccttcatttt tcaatgtttt taaaacactg gtttgggggt aaaattcgat
     4081 aaatggatca tcgagaagat tttgaagcaa atagaaatcg aattcctgcc tgtttaccgt
     4141 atagcccatt ggttgcacat catttttttg caggtcgagt aaaaatgaat aattgttatg
     4201 cttcagggtg ttgatctgaa atccataaat aggattttta ttatcgaaac catcgagcaa
     4261 ttttttgtta ttgaaactca gctttcgcaa tgtgttgata gctcttattc ccagttcgcc
     4321 tccgcagggt ttcgatcgtg ggaaagcttt tttatcgaaa atttttattt tgagccctct
     4381 tcccttaagg gtaagtgcac atgccaatcc tgctggccct gcaccagcta tggccacatc
     4441 acaatcatca ggcgaatcca tcgcagatcg ggtattttaa gagatttacc ttgcgaaatt
     4501 aaagaataaa gttgtaattt ctatcgaaac aaaggaaatg aattggaggg tacaacctaa
     4561 aatcaataaa aaacccgccg tagaggcggg ttcaatggga ctaccggcga gcggtttggt
     4621 ttgtttacat ttttaagtaa agggcccgtt aaatattaat atctgcggtt accttggtag
     4681 ccaccacggt tgtcgtttcc accccggtgg ttgtttctgc gaggaccttc tttacgttct
     4741 tcggctttgt taacaacgat agttctacca tctacttcag caccgttaag ttcgtcgatt
     4801 gcctgctgag cttcagcatc attgggcatt tcaacaaaac caaaaccttt actgcggccg
     4861 gagaatttat ctgtgatgat cttcaccgaa gttacttccc catgttcttc aaagatttct
     4921 cttaaatcag tctcttgtag cttgaaaggg agactgccaa caaaaatgtt catacaaaaa
     4981 caaaaattaa gtgagtaaat taaaaacgtg caaagatata aactaatcta aacgagtatt
     5041 catttcggcg aaaaattgaa aaaatttttc caacttactc agcgaaaaaa atttttttac
     5101 ccggaaattc aaacaattga tgcgactttt cgaatctccc atccaataaa aatcttggct
     5161 ttttgatgaa ttcaatttcc taatcgaccc tgtcttgcaa tatatatccc atacgctgta
     5221 aacttttcgc ctcattgcct atttttaatc accattcacc aaaccccatc ttctttgcca
     5281 gcctcttcct tgctgtaata gaatgcagtg ggatgtggta gtttaaactt ctcccgggca
     5341 atcttctcgg gaaacaagta atatataatc atagccctgg tacaaacccc agtatcttct
     5401 ttcgaaagcg taacttctat aaatgcaaga tgatgcttct gttcgatcaa gcgtgcttct
     5461 aaatgaatat tactgccaat tccacttacc ggttgtaaat accgaaattt catcgatgca
     5521 gttactccac ctgtttttag tttcacaaat acataccaac ttgcaatttc atccatcaaa
     5581 caagcctgaa ttcctccatg gagtatatcg tggtagccac aataattctc agtgaaaagc
     5641 cactcggctg taatagcatc atccgtttcc ttgaacttca agtgaagacc gtaaggatta
     5701 gtgggcgaac atccaaaaca atggttatcc tcctcatcaa cgaaaggatt acgtatattt
     5761 ctcatcttat cctgttcgat tcgttgcaaa gttaaacctt cctctagcat aatctattgt
     5821 tgattcttct atgcatgatt aaccatctgt ttcataattt attatggttt ataattcgtt
     5881 ttctgatatt caacaaagaa gtctataccg attcgaaaaa tctttggggt attgcgaagc
     5941 gtatcgatcg ggttcaaact ggcgctggtt ggcttctcct gaaatcaatg gtttctaaag
     6001 ctgaaaaatg taaggtttaa gtgatattat caatgagaaa atcctccttc attatataac
     6061 tcaataatta tgagaaactg attgaaattc attagtatat aaattagtcg agggttttct
     6121 tttggctttg atttttcgag ctggatgaca gaaaagactc aacaaccagt tttattttaa
     6181 atttgcagtg gaaacggaat aaaatcattt tcgcatgaaa ttattggaag gtaaaactgc
     6241 tttgattact ggagcggcca gaggtatagg tcgttctatt gccttgaagt ttgcttcgga
     6301 aggggctcac attgcctttt ccgatcttcg tttggacgag gcaacgcaaa ttttggaaga
     6361 agaaatccga ggaatgggtg tcgaagctat ggcttatgct tcggatgcgt cttctttcag
     6421 tgacagtgag atattggtta cgcaggttat agaggatttt ggtacgattg aaattttagt
     6481 gaacaatgca ggcattacgc gtgataattt gttaatgcgc atgagtgaag ctgactggga
     6541 cttggtaatg aaagtaaatt tgaaatcggt tttcaatcta accaaagctg ttcaaaaaat
     6601 tatgttaaaa aacagattcg gaagcatcat caacatgtcg tcagttgttg gagtgaaagg
     6661 gaatgctggg caagccaatt attcagcttc aaaggctggt atcattggtt ttacgaagtc
     6721 aatggcacaa gaactgggtt cgcgtaatat tagatgtaac gcagtggctc ctggttttat
     6781 tgatactgaa atgacggcga aactttcgga ggaagtaaaa agggaatggg ccgaaaaaat
     6841 tcctttgagg cgtgctggtc tgcccgaaga tgttgccaat tcctgccttt tccttgcttc
     6901 tgatttgtca tcttatataa ccggccaagt gatacatgtt tgtggcggaa tggttatgta
     6961 aacaacatca ctcattaaga tgtcgtttat tttcgaaaat tccggctggt ggttggtgtt
     7021 agcccctgtg gtgggcttcg tttatgcttt tgtgttatat cgcaacacac acgatatctt
     7081 cgaccgaaaa atccagattc tgctcggaat attacgtttt gtagtggtta caattctggt
     7141 tatcgttttg atgggagtac tcataaaact tacctcacat cgtttcgaaa agcctttggt
     7201 ggttttggcg ctcgacaata gccaatcaat ggctcaatta cctgattcga ccgaaatcag
     7261 gaagaattta cctggacttt tgaaaaatct cgaatcaaaa ctcgcacccg aatttgaagt
     7321 taagaaagtg agtttcggtt catcggtaaa tgacactttg aacttcaggt tcgacgaatc
     7381 tgaaaccaac atggcgtctt tgctcaccta tattcaagaa cgctatccca atagaatcat
     7441 cggtggatta gtccttgtct cagatggaat catcaaccga ggacaagatc ctttgaccat
     7501 ggctgaatcg aagttatacc caatatttcc catagctatg ggtgacactt caactcgaag
     7561 agatttgttt tttcaagaag tacgttataa tcagattact ttcaccggga atcaattccc
     7621 tgtcgaaatt tatttaaagg ccgaatcgtg taatagccag aaatcgtcag taactatcag
     7681 cagcaataat aaaaaattgt ggacatccga ttttacggtt tcttccgaaa atcaaatcat
     7741 aacattttct cctgccttaa tggccgaaaa acctggtata atccgctata caatcgaaat
     7801 aaaacccatt gaagaagaga gcaatgtaaa gaacaatcgg atggattttt ttatcgaggt
     7861 aaaagacacc agagtaaagg tgttgattct ggccgatgct cctcatcccg atatagcagc
     7921 tttgaagaaa gtatttattt ttcgggatat ttatcaggcc gaagattatt taatctccaa
     7981 tttcgataag gatatccacc aattcgacct catcatcttt catcagcttc cttccactcg
     8041 tgtttccgta aatgatattt acgagcaagc gctgaaggca ggcaaacccg ttatgtttat
     8101 tcttggccag agttcggata ttgaactgtt gaatgccatg gaaatcggat tgaaggttac
     8161 agcaacagga aagaaattcg taaatgctta tccactgctg gtcgccgatt ttcaactttt
     8221 ccgtacagat catcgtttgg tagagacgtt cgacaaactt cctcctttga tttcacccga
     8281 tggaaaatat tctgtttcgc cgattgccgt tccttatcta ctacagaaaa ttgataatgt
     8341 actgacatcg aatcctttaa ttctttttca tcgcaaggaa aaccagtctg ttgcaataat
     8401 tgccggtgaa ggcctttggt ggtggcgcaa tattttgtat cagaattatc gggatcatga
     8461 aatattcgaa gattttattc ataacatggt tcagtatcta atcagtagcg aacctacggg
     8521 aaatttcaga gtaaaagttg gaaaccgttt tcgtggcaac gaacatgtaa agttcaatgc
     8581 cgaattgtat gatgccactt tcaagttagt gaacgaacct gaagttcacc tcgagataaa
     8641 ggatgaaaac ggtgtgagat atccttttat ttttaataga ataggggata tctatgagct
     8701 cgatgccggg atattcaatc cgggaatcta ttcatggact gccagaacca ccttcaacaa
     8761 ggaagaatta tcggctggag ggatttttgt tgtagaacat acaattgccg aaaaaaacta
     8821 cttaaaggct aatcatcgtc ttcttttcga tttgagtgaa aatagcggag gaaaaatggt
     8881 ctggcctgtt cagaccgata gtattcccca atggttgaaa aaaatgggaa ataccgctac
     8941 taaagtctat accgaagaat cttatgttaa actgaatgat ttagggtggc ttttggcatt
     9001 gatgatcatc ctcatatcgg gagaatgggc aataaggaaa tggtcgggaa tattatgatc
     9061 agatgaggcg aataaaaatt tagatcaata aatatgaata ctactgttat ttatttgttt
     9121 tatgctttct tggctatttt aatactgagt tttgcagccg aaaggatcct ggactggttg
     9181 aacattagga attggcccaa ggtattaccc gatcttattc gagatatatt tccggaagag
     9241 aaatttatgg aagtccgaca atatcagtta gtaaattata aaataggatt catcgaagcg
     9301 acgattactt ctatcattac cattgggttt ctatattttg gtggatttgc atggctggat
     9361 ggagtagcac ggtccttttc atcaaattta atcgtacagt ctctgatttt ttttggaatt
     9421 atagctgtag ccacatttat catcaggctt ccgttcgata tctatgatac ctttgtgata
     9481 gaatcgcggt ttggattcaa taccactact cccagaatct atattactga ccagctaaag
     9541 tcggcattgt tgaccatctt gatcggagtt ccattgtttt cgcttattgt ttttctttat
     9601 caaaagaccg gagaatattt ttgggtactg gtttggttgg tgattagtat ttttgcatta
     9661 tttatgtcgt tgttttacag tagtttgata gtgcctttat tcaacaagca aacccccata
     9721 cccgagggag aattacgcac tgccatcgaa gaatttgccc ataaagcggg atttaaggtg
     9781 aaggatattt acgtgatcga cagttcgaag aggagcagta aggccaatgc ttattttacc
     9841 ggccttggac ccgaaaaaag aatcgttttg tatgatacgc tgataaaaga ttttagtatc
     9901 gatgagattc tggctgtttt agctcatgaa attggacatt acaagcatca tcataccacg
     9961 atagggctta taacgagcat tgttcagact gggttaatat tgttcgtatt ttctcttttc
    10021 gttaaacccg ggtcggaatt gacggaagtt ttatgcaatg ctgcagcagg tgccaggcaa
    10081 gtaactcctt cattttatat gggattaatt ggctttactc ttttgtttag ccctatctcg
    10141 gccatcatcg gattaatctt caattctata tcacgcagac atgaatttca ggccgatgcc
    10201 tatgtagcag aacatcaaat gcagggctat ttgcaatcgg ctcttaaaaa attatctgtc
    10261 aatcatttga gtaatccctt tccgcatcct ttgtacgtaa aagtgtatta ttctcatcct
    10321 ccattgatag aaagactgaa ggcattggaa aacactagtc gaagagaacc tcaagggaat
    10381 ggtttttaga aatagaagct gcgggcttaa tttttcggaa gaaagattgg ctaagtataa
    10441 atactgagat aatatgattt ctactttctc atttaattct tagcccgaat ttgccatgag
    10501 ggtagagatt caatataaga aagaataggg gaaacatcga gaattatttt cttttttcga
    10561 ggtttcacag tttcgaagaa ttacatagct ttatataaaa taggggaggg ttacaatcgg
    10621 atgaagaaac tggccttgat catgaattta ggtatttttt tgccatcggg acccgggtcg
    10681 aggtatgata atcgatgaaa ggcctgaaca cgtatgattt tgaggatgtt ttctatacca
    10741 tatgaaactt ccatgtaggg tttgccaaag tccatgatac ggaactctgt gattggattt
    10801 ccgttgatag agggagccaa taaacctttt ggattatcgt ttgtacgtat ataatttttc
    10861 tttccatagg ttg
//
LOCUS       DAOORU010000073        10860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7567_length_10860_cov_5.764368, whole genome shotgun sequence.
ACCESSION   DAOORU010000073 DAOORU010000000
VERSION     DAOORU010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10860
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7567_length_10860_cov_5.764368"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..847
                     /gene="aat"
                     /locus_tag="PLF20_05940"
     CDS             152..847
                     /gene="aat"
                     /locus_tag="PLF20_05940"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HOX80577.1"
                     /translation="MAIIALEPKYEGYLISKKAEEEGLVAIGGHLSVKSLLCAYSSGI
                     FPWYAEGKPIMWWSPDPRTILIPEKFKPSHSLRQLIKQRRFRITCDTAFREVVHHCGI
                     VPRKGQDGTWITPEVKKAYHKLHKAGYAHSFETWLGDRFVGGLYGVSLGRAFFGESMF
                     YLENNASKVAMYYLVEFAKCHGFLFIDAQIETTHLLNLGAENIPRREFLAALHHALQF
                     PTLKGEWRIPDEK"
     gene            complement(945..1403)
                     /locus_tag="PLF20_05945"
     CDS             complement(945..1403)
                     /locus_tag="PLF20_05945"
                     /inference="COORDINATES: protein motif:HMM:NF017970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HOX80578.1"
                     /translation="MKYHEIKSLEEIKRQYHPIHNVNEQYREKLSVLDKLALWITIHV
                     GSMGFFLIIFCWTVFWLSWNILSPPHLRFDPYPAFVLWLFISNMIQILLMPLIMVGQN
                     LQARHSELRAEHDYHINQKSETEIETLIQHMENFSEEIQEIKIKLEKIQS"
     gene            1697..3439
                     /locus_tag="PLF20_05950"
     CDS             1697..3439
                     /locus_tag="PLF20_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylase"
                     /protein_id="HOX80579.1"
                     /translation="MTKRRILIRDLTLRDGQQSLFATRMTQEQIDELLPLFKEAGFYA
                     MEVWGGAVPDSIMRYLKEDPWERLEKISKAIGNSSKLTALSRGRNLFGYNPYPDEVIE
                     GFLRNAVESGIRIMRVFDALNDTNNIESSIRFLKKYGATIDAALCYTVDPKFSTRARV
                     KAFLHGKSLPNRIFTDDYFVNLARELEAMGADMLTIKDMAGLITPMRTYRLISRLKSE
                     LSIPIDFHTHCTPGYGLASSLMSLLANIDILDTSILNFAGGPGAPAFEIIQIFCNKLN
                     IETDLNLKVIGNINEKLGEIRKSLSTYDTAPYLPRPFHIATDDIPLEIHNLFDKAIAA
                     AQNKNQDELLESCHAIEDYFQLPPPDEKIREAEIPGGMYTNMLSQLKQVRLENLLPRT
                     LELIPEVRLDAGLPPLVTPTSQIIGSQAVNCALDETKGQPMYTNRSLQFVNLVKGIYG
                     KTPYPIDPEFRYKIAGVREETPYDFRFYKKQPNPVFEEYGGIKLANNEKEELLLELFP
                     NVAADFLKNRVEQIYMAKIHQIEEEKHRKLEEEKAAYEKLTPEEKRQRLLNGLYNYKW
                     TSYQDDEEEALGHS"
     gene            complement(3587..4336)
                     /locus_tag="PLF20_05955"
     CDS             complement(3587..4336)
                     /locus_tag="PLF20_05955"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOX80580.1"
                     /translation="MSGHNKWSTIKRKKGALDTKRSKMFSKIIKEITVAVKENGPDPE
                     SNPRLRLAIANAKGVSMPKENILRAINKGSDKDAANFQETTYEGYISGVAVFIECTTD
                     NVQRTVSNIRSIFNKFGGSLGVNGSLAHIFERKGIFTIPISQIKDMEEFELEVIDAGA
                     EDIDVDEENGIVTINTAMEDFGSMIKKLESLHIDPENAQLQRIPVSTIEVDPETAKKL
                     LRVIDLFEEDDDVQNVYHNMSLTDEILASFE"
     gene            complement(5022..6662)
                     /gene="hcp"
                     /locus_tag="PLF20_05960"
                     /gene_synonym="priS"
     CDS             complement(5022..6662)
                     /gene="hcp"
                     /locus_tag="PLF20_05960"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011202759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOX80581.1"
                     /translation="MFCFQCQEATKGVGCTVKGICGKTPQVANLQDLLLFVLKGIAIY
                     SSALRRAGEKNLPNRFLVEGMFVTITNANFDADAIRERITKGLQIRDRLKVRANELGL
                     HPEFSDHDAATWLAVEDKYEIIAKQVGVLKTEDPDIRSLHELLIYGLKGMSAYTEHAM
                     NLEFEDEALFSFLEEGLKATLNPNLSLDELTSLILECGRHGVLAMSLLDRANTTRYGN
                     PEITKVNIGVGKNPGILISGHDLKDIEELLAQTEGTGIDVYTHSEMLPAHYYPEFKKY
                     RHFVGNYGGSWWKQNEEFETFNGPILFTTNCIVPPRSTSTYSNRVYTTGAAGHQGFKH
                     IPDRIAGKPKDFGEIIEHAKNCKPPVEIEQGEITGGFAHEQVFALADKIVEAVKSGAI
                     RKFFVMAGCDGRLKSREYYTEFAQKLPKDTVILTAGCAKYRYNKLSLGDINGIPRMLD
                     AGQCNDSYSLVMIALKLKEIFGLEDINQLPIAYNIAWYEQKAVIVLLALLSLGVKNIH
                     LGPTLPAFLSPNIAKVLVDNFGISPIGTVDEDLELFLS"
     gene            complement(6807..7136)
                     /locus_tag="PLF20_05965"
     CDS             complement(6807..7136)
                     /locus_tag="PLF20_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOX80582.1"
                     /translation="MGNTLFSAGEIMQFTQKVEYSKEGIVSKVVIDKSSGTITLFAFD
                     QGQRLSEHSAPFDALVQVIEGEAEIIINGKSFMLKAGDSIIMPAGIAHAVNAPDRFKM
                     LLTMIER"
     gene            complement(7366..7764)
                     /locus_tag="PLF20_05970"
     CDS             complement(7366..7764)
                     /locus_tag="PLF20_05970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOX80583.1"
                     /translation="MSKVVTLSEAASLAIHAMVIIGRSGNTINAQLMAQRMHSSRNHL
                     AKVMQRLVKGGFVKSARGPSGGFVLNKSANEISLLDIYECIEGPIELLECPVGNKICP
                     MGSCLMEGTIHEATYLIKDYFARKKLSDFL"
     gene            8240..9700
                     /locus_tag="PLF20_05975"
     CDS             8240..9700
                     /locus_tag="PLF20_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX80584.1"
                     /translation="MRDDFEEEDDRDELSELLDRFEKMMEEQVEYFFDAEDFEDIIDY
                     YLEQHDSPRAQAAIEAAQHQYPYNSIFELKKAQLLASTNQEEKALKILSRLERIFPNN
                     FEIHYTRSFIYSKLSQHKLAIKELKKALKLTDNPYEILVTLAFEHEKIWEYEEAIRQL
                     EKALEYDSTEETIINELASFYDMAENLDKGVSYFQKFVEKNPYNVPGWFNLGILYNKL
                     SLYEKSIEAFDFAIAIDPEYSPAYFNKGNAYASASMYEKAIETYRESLQHEAPDANAY
                     CCIGECYQKLQNYDDALFYFKLATKLDTETYYAWYGIACIHYLQGNLKPALVYVQKAL
                     HYFEEFERIKYLEGDIFMALGKYDKAIDTYNRVAANIPKNHDIWFDLASAFDAINDSA
                     SAISVLKEGCSYHHNENIDLLYYCIEYIIEHDSVAHALPYIKIAYPLQPSIIEILFMK
                     FPMLKNNKEYLSFLNSLFDKGDNSISEKDDSSPSIN"
     gene            9767..10531
                     /locus_tag="PLF20_05980"
     CDS             9767..10531
                     /locus_tag="PLF20_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HOX80585.1"
                     /translation="MDNGFSHIYGLIGYPLEHSFSPAYFRQIFEKSGMNNCDYRLFPV
                     RDIESFSFILNDIPHLYGLNVTIPYKEAIIPWLNELSPEAKLIGAVNVIKIIHKGTKI
                     QTIGHNTDAAAFIRTLLPLLRSHHSQAMIFGCGGATKAVAYSLSLLGIPYIIVSRGKK
                     GPHIISYEELSISLINKYPVLINCTPVGMFSYPDEALSIPFEGIGPRHLVYDLTYNPP
                     LTQLLKIAQQRGATTANGLQMLHYQADLSWEFWNDR"
     gene            complement(10537..>10860)
                     /locus_tag="PLF20_05985"
     CDS             complement(10537..>10860)
                     /locus_tag="PLF20_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOX80586.1"
                     /translation="GSHLHFEVRYFDKAFDPEKIICFETGELKASSIYLDDEFLTTRT
                     RLTAKSRPQVESVGSEEIFHTVKKGDTLSKIAQKYGITIAAICRLNNIRPKNIIKPGQ
                     VLRVN"
BASE COUNT         3314 a         2098 c         2054 g         3394 t
ORIGIN      
        1 atatctttgc ccatggttgt taatttaggt ttactacaca aatttacaac tggggatgaa
       61 acttcggttt tgtcctttct tctttttatc caattttttt gtcggtcagt agtgatattt
      121 tttaataaat agacccgatt ttatattgca gatggccata attgctcttg aaccaaaata
      181 cgaaggatac ctgatttcga aaaaagccga ggaagagggt ttggtagcca ttggaggcca
      241 tctttcggtc aaatcgttgt tgtgtgcata ttcatccggt attttcccat ggtacgcaga
      301 agggaagccc attatgtggt ggtcgcccga tccacggact attcttattc ctgaaaagtt
      361 taaaccctca catagtctgc gacaattaat caaacaacga agatttcgaa ttacctgcga
      421 tacggcattc agagaggttg ttcatcattg tgggattgta cctcgcaagg gccaggacgg
      481 cacatggata actcctgagg ttaaaaaagc ttatcataaa ctacataagg ccggatatgc
      541 acattctttc gagacctggt tgggcgatcg ctttgtggga ggtctatacg gagtttcatt
      601 aggaagagcc tttttcggtg aatccatgtt ttatctcgaa aacaatgcat caaaagtagc
      661 tatgtattat ctggtggaat ttgccaaatg tcatggcttc ttatttatag atgcccaaat
      721 agaaaccact catcttttaa accttggggc cgaaaatata ccgcgcagag aatttctcgc
      781 ggctctccat catgctttac agttcccaac tttgaaaggg gaatggagaa ttcctgacga
      841 aaagtaataa caacccgggt atctttgaaa tcaaccagac atttggcagt tgatatatgg
      901 gtaaaaaatt cgactcgatt aaatacaggc ctatctaatc cctattagga ttgtatcttt
      961 tctaacttta tctttatttc ctgaatctct tccgagaaat tttccatgtg ttggattaat
     1021 gtttctattt ctgtttccga tttttgattg atgtggtaat catgttcggc tctgagttct
     1081 gaatgccttg cttgcaggtt ttgacccacc attatcagag gcatgagaag aatttgaatc
     1141 atattcgaaa taaaaagcca tagtacgaat gcaggataag gatcgaagcg cagatgaggt
     1201 ggggaaagaa tattccatga aagccagaaa actgtccagc aaaaaataat caaaaaaaat
     1261 cccatacttc ctacatggat ggtaatccat aatgcaagct tatcaaggac tgataatttt
     1321 tccctgtatt gctcattgac attatgaatc ggatgatact gccttttgat ttcctcgaga
     1381 gatttgattt cgtgatattt cattttcaat cattttttaa tgaaaacaat attataaaga
     1441 tgtaactcgt ttttcgataa agggagaaaa gtgatttagg gctatgaaat tataactttt
     1501 tcacgctttg cgaatatgca cctttagaat tttgattaaa accttattga cattataata
     1561 tgcagataat caaaatttat tcatcaatca aataacaatc gaccattgcg ttccggaatt
     1621 ccctttacct ttgaagacag aaatagatag tgatttttat tttttaagct attattaatc
     1681 agcaacataa tattttatga cgaagagaag aattttaatc agggatttaa ccttacgcga
     1741 tggacaacag tcattatttg ctacgcggat gacgcaagaa caaatagatg agttacttcc
     1801 gttgttcaag gaagcaggtt tctatgccat ggaagtatgg ggaggtgctg ttcctgattc
     1861 aattatgcgt tatttgaaag aagatccatg ggagagactc gaaaaaataa gtaaggcgat
     1921 cggaaattct tctaagctca ctgctttgtc gagaggaaga aatctcttcg gttataatcc
     1981 ttatcccgat gaagtcattg agggattttt acgaaatgct gtcgaatcgg gaattcgaat
     2041 catgagggtt ttcgatgccc tgaatgacac gaataatatt gaatcatcca ttcgattttt
     2101 aaaaaaatat ggtgcaacga tagatgccgc tctatgttat acggtagatc ccaaattctc
     2161 gacacgtgca cgtgttaaag cttttttaca tggcaaatct ttacccaatc gcatctttac
     2221 cgacgattat tttgtcaacc tggccaggga actcgaagct atgggtgccg acatgcttac
     2281 cataaaagat atggcaggct taatcactcc tatgagaact tatcgtctga ttagccgatt
     2341 gaaatcggag ctttcgattc caattgactt ccatactcac tgtacaccgg gttatggctt
     2401 ggcttcgtct ttgatgtcac ttttggccaa tatcgatatt ctcgatacct cgattctgaa
     2461 ttttgccgga ggtccaggag ccccagcctt cgaaatcatc caaatcttct gcaacaaatt
     2521 aaacatagaa acagatttga atttgaaagt tataggtaat atcaacgaga aactcggtga
     2581 aatcagaaaa tcgctttcga cttacgatac tgctccttat ctccctcggc ctttccatat
     2641 tgctaccgat gatatccctc tcgaaattca taaccttttc gataaagcga tagcggctgc
     2701 ccagaataaa aatcaggatg agcttctcga atcctgccat gccatcgaag attattttca
     2761 actacctcct cccgacgaaa aaattagaga agcagaaatt cctggcggaa tgtacaccaa
     2821 tatgttatcg cagttaaaac aggtaagact cgaaaatctt ttaccacgta cactcgaatt
     2881 aattcccgaa gttaggttgg atgcagggct gccccctctg gttacgccta ccagccaaat
     2941 tatcggttcg caagccgtaa attgcgcgct cgacgaaact aaaggccaac ctatgtacac
     3001 aaaccgctca ttgcaattcg tcaatctggt gaaaggcatt tatgggaaaa cgccttatcc
     3061 aatagatcct gaattcagat ataaaattgc cggcgttaga gaagaaacac cttacgattt
     3121 tcgattttat aaaaaacaac ctaatcccgt attcgaagaa tatggcggaa taaaactggc
     3181 aaataatgaa aaggaagaat tattactaga actttttccc aacgtagctg ccgatttcct
     3241 gaaaaaccgg gtcgaacaaa tttatatggc aaaaattcat cagattgaag aagagaaaca
     3301 taggaaatta gaagaagaaa aagcagctta cgaaaaatta actcccgaag aaaagcgcca
     3361 aagattgctt aatgggttat ataattataa atggacttct tatcaggatg atgaagaaga
     3421 agctttaggc cactcataat tgaaatttaa aaacattgat cttacaatgc tgaattcgat
     3481 tggttttttt aacttgattg agttggcttt ctatgtattt ctggaggaag atataatctc
     3541 tgttcctcgt cctgaatgtt gagatacaat gatatagata atcagtctat tcgaaagagg
     3601 caagaatttc atcggtgagg ctcatattgt gatatacatt ctgaacatca tcgtcttctt
     3661 cgaaaaggtc gattaccctc aataatttct tagcagtttc tggatcaact tctatcgtcg
     3721 agacagggat gcgttgtaat tgggcatttt ctgggtctat gtgaagagat tcgagttttt
     3781 taatcattga gccaaaatct tccattgctg tattgatagt cacgatccca ttttcttcat
     3841 ccacatcaat atcctcggca cctgcgtcaa tcacttcgag ttcgaattct tccatatctt
     3901 tgatttgaga tattgggatg gtgaagattc ctttccgttc aaaaatatga gccagagaac
     3961 cattgacgcc aagactacca ccaaatttat tgaaaatgct acgaatgttc gataccgtgc
     4021 gctgaacatt atcggtagtg cattctatga aaacagcaac gccgctgatg taaccttcgt
     4081 aagtggtttc ctgaaaattg gcggcatcct tatcggaacc tttgttgatg gctcttagta
     4141 tattttcttt tggcatgcta acgcccttag cattagcaat agcaagacga agccgtggat
     4201 tcgattcggg atcagggcca ttttctttga cagctactgt gatttccttg atgatctttg
     4261 aaaacatttt cgagcgtttc gtatcgagcg ctcctttctt tctcttaatc gttgaccatt
     4321 tattatgacc tgacataaaa tgagttatta aaatgttttg aacggaaaaa tgggattcaa
     4381 aattacaaaa aaaatgggtc gagactcata aaggcattta tgaagtggag gaatttatat
     4441 tttatatgtc tgtctccgtt tatggggcag acgtataaag tttgcccttt tttaaataat
     4501 ggactgcata caataaattt ggcgtacagt gtgcattcag catctattta tccttgttct
     4561 cttctggcac tttttgcttc aatacagcaa aatagcaaag atatatatct gccctgtctt
     4621 tacttgaaag ttcggaagga ggatttctct ggttggcaaa ctgcttttcg ctgacaattg
     4681 aacataccaa aaatagtctt cctgcccgaa aaggttggag ctctataacc gattttggat
     4741 gattatcaat gagccatctt ataattttcc atattcaaat tccctggttt gggctgtagt
     4801 agagggggga ttaatacatt attgctcatt cttgtcttgt tttgttaatt gaagctaagt
     4861 cgatattcca tgattttaaa atctgaatga atattactgt atcatataaa gataaaagat
     4921 cgcagatccg aaattttcat catgatattg tatggtaaag tctaaagtcg aaatgagcgc
     4981 cgggctttgc ccagcgctca taaacattca acatttcaaa ctcagcttaa aaataactcc
     5041 aaatcctcat caacagtccc tattgggctt attccaaaat tatctaccaa taccttagct
     5101 atattcggtg ataaaaatgc cggcaaggtc ggtccaagat gaatgttctt cactccaagt
     5161 gaaagtaatg ccagaagaac aattacggcc ttttgttcat accatgcaat attataagct
     5221 ataggaagct ggttaatatc ttccaatcca aaaatttcct tcaacttgag ggcaatcatg
     5281 accaagctgt acgaatcatt acactgtcct gcatcgagca ttcgtggaat tccgtttata
     5341 tccccaagag acagtttgtt gtagcgatat ttagcacaac ctgccgtaag tattactgta
     5401 tctttgggga gtttttgggc aaattctgtg taatattccc tgctcttgag ccttccatcg
     5461 catccggcca tgacaaagaa ttttctgatg gcaccagatt ttacagcttc tacgatttta
     5521 tcggcaagtg caaaaacctg ttcatgagca aaacctcctg taatttcacc ttgttcgatt
     5581 tcaacgggag gtttgcaatt ttttgcatgt tcgataatct caccgaaatc tttgggcttc
     5641 ccggctatac gatcaggaat atgcttgaag ccctgatggc cagctgcacc agtagtataa
     5701 acccgattgg aataagtaga agtggaacgt ggcggtacta tgcaatttgt ggtaaacaga
     5761 ataggaccgt tgaaagtttc gaattcttca ttctgtttcc accacgaacc gccatagttg
     5821 cccacaaaat gcctatattt cttgaattct ggataataat gtgcaggcag catttcgcta
     5881 tgtgtataga catcgatacc tgttccttct gtctgtgcaa gtaactcttc tatatctttc
     5941 aggtcgtgac cgctgatgag aatacctgga ttttttccaa caccaatgtt tactttcgtt
     6001 atttcgggat tcccataacg agtagtattt gccctatcga gcaacgacat ggccaaaact
     6061 ccatgccttc cacattctaa aataagagaa gtgagttcat ctaaactaag attgggattc
     6121 aatgtagctt tcaatccttc ttcaagaaaa gaaaacaggg cttcatcttc aaattcaaga
     6181 ttcattgcat gttcagtgta ggccgacata ccttttaaac catatatcaa cagctcatgc
     6241 aatgaacgaa tatctggatc ctcggttttc aaaacgccta cctgtttcgc aataatctca
     6301 tatttatcct ctacagccaa ccatgtggct gcatcatgat cggaaaactc gggatgcaat
     6361 cccaattcat ttgcacgaac cttcagtctg tcacgaattt gcaatccttt tgtaatgcgc
     6421 tcacgtatgg catcagcatc aaaattggca ttagtaatcg taacaaacat tccttcgacc
     6481 aaaaagcgat tgggaaggtt cttttctccc gctctacgta aggcagaact ataaatcgct
     6541 atgcccttca atacaaataa caaaagatct tgaagattag caacttgagg cgttttgcca
     6601 caaattcctt ttactgtaca tcctacccct ttggtagctt cctgacattg aaaacaaaac
     6661 atctcaggtc tggaattcga tagatggttt ccttcggttt cttctaaccg taaataatga
     6721 ataaaactca tagcattaaa gtttgaagtt aaaataattg aacgattaag tttataaacg
     6781 tttatacata agatcaatca aaagaatcaa cgctcaatca tggttaaaag cattttgaat
     6841 ctatcgggtg cattcactgc atgtgcgatt ccagcgggca tgataataga atcgccagct
     6901 tttagcataa aagattttcc attgataatg atctcagctt ccccttcgat aacctgcaca
     6961 agggcatcga aaggcgccga atgctcactc aagcgctgac cctggtcgaa cgcaaacaag
     7021 gtaatcgttc ccgatgattt gtcaatcacc actttactca cgattccttc ttttgaatac
     7081 tctacttttt gggtaaattg catgatttca ccagcagaaa ataacgtgtt tcccattttt
     7141 gttataatta attgttattt aattgattat aatacaactt tctttctttt tcattctgag
     7201 aaaattttcc taaaattgaa aaatttgatt tcaatccatt gcttaataac gtgttataaa
     7261 tttttttata tttataataa ttcggtattt caatacgcaa atatataaaa tattcaatta
     7321 ccaaacgatt ttgaaaattt atttagaaaa attaaacttc gtgatttaaa gaaagtccga
     7381 tagttttttc cgagcgaaat aatctttgat taaataagtt gcttcgtgaa tagtaccttc
     7441 cataagacag cttcccatgg ggcaaatttt atttcccaca gggcattcca aaagttcgat
     7501 tggtccttct atacattcgt aaatgtcgag cagtgaaatt tcattggcag atttgttcaa
     7561 tacaaagcct cccgaaggcc cgcgggctga tttcacaaat cctcccttca caaggcgctg
     7621 catgacttta gccagatgat tacgtgaaga atgcattcgc tgagccataa gttgagcatt
     7681 gatggtattg cctgaacgtc caatgattac catagcatgt attgccaatg aggctgcttc
     7741 cgaaagggtg acaactttac tcattcgaat gaaaaatgaa aatgaatgat agtaattctg
     7801 tacacaaatt tacacacaag ggagaaattt tgagtcaatt ctgcttacgt aagtatatat
     7861 ttaattgatt cacagcagta taaatttctt aaaaaatgat attagatgga aatcttactt
     7921 ctgcatttga gaatcggaag cggagcactt aaatgattta tcttggaata agattaaaaa
     7981 gatgagccga aacgatttac gatgtccttt atcacgataa ttcattttaa tcgtctcaga
     8041 ttctcctatt aaaaagctca aaattttttt atatttatta tgaatcattc agatcatttg
     8101 tttgggtatt gggtgtttct gatggaacac aaaagcagtt taccggtatt gaggaggcac
     8161 aaccgccata gggaggaaat ttcatttttc taagttttcc tcgggatttt gtgtaagttt
     8221 gcaatgtatt ttttgaacta tgagagacga ttttgaagaa gaggatgata gagatgaatt
     8281 gtcggagctc ttagatcgtt ttgagaagat gatggaggag caggtggaat atttttttga
     8341 tgcagaagat ttcgaggaca tcatcgacta ttatctcgaa cagcatgata gcccgcgagc
     8401 ccaggcagca attgaggcag ctcagcatca gtatccttac aatagcatat tcgagctgaa
     8461 aaaagctcaa cttttggctt ctaccaatca ggaagagaag gcgctcaaaa ttctttcacg
     8521 attggaacgg atttttccca ataatttcga gattcattat actcgcagtt ttatttatag
     8581 caaattaagc cagcacaagc tagctatcaa agaattgaaa aaagccctga aacttactga
     8641 caatccttat gaaatattag ttactttagc tttcgagcat gaaaaaatct gggaatatga
     8701 ggaagccatt cgacagttgg aaaaagcgct cgaatacgac tcgaccgaag aaaccatcat
     8761 caatgagctg gcatcatttt acgacatggc tgagaatctt gataaaggcg taagttattt
     8821 tcaaaagttc gttgagaaaa atccatataa tgttcctggc tggttcaatc tgggaatttt
     8881 atacaataag ctgagtctgt atgaaaaaag catcgaggct tttgattttg ccattgccat
     8941 agaccccgaa tattcgcctg catatttcaa caagggaaat gcttatgcca gtgctagcat
     9001 gtatgaaaag gccatcgaaa cttacagaga aagtttacaa cacgaggctc ctgatgcgaa
     9061 tgcctattgt tgtataggcg aatgttatca aaagctccaa aactatgatg atgcgctctt
     9121 ttattttaaa ttggctacta aactcgatac cgagacttat tatgcctggt acggaatagc
     9181 ctgtattcat tatttgcagg gaaatttaaa acctgcattg gtttatgtac aaaaagccct
     9241 ccattatttc gaagagttcg aaagaataaa gtacctcgaa ggggatattt tcatggcgtt
     9301 gggaaaatat gacaaagcta ttgatacata caatcgggtg gcagcgaata taccaaaaaa
     9361 tcatgatatc tggtttgacc ttgcttcggc cttcgatgcc atcaatgatt cagcttcggc
     9421 catttctgtt ttgaaggaag gttgctctta tcatcacaat gaaaatatcg acctgctcta
     9481 ttattgtatt gaatacatca tcgagcatga ttctgtggct catgccctcc cttatataaa
     9541 aatagcctac ccgttacaac catccatcat cgaaatcctt ttcatgaaat ttcccatgct
     9601 aaagaacaat aaagaatact tgtctttcct caattccctg tttgataaag gcgataattc
     9661 aatctctgaa aaagacgatt cttcaccttc aataaattga gataattact tccatgcggc
     9721 tcgattcgtt cgaacgaaaa tctattggaa gtcttttcat aatacaatgg ataatggatt
     9781 tagccatatt tatggattga taggatatcc cctcgaacac tccttctctc cggcatattt
     9841 tcgccaaata ttcgaaaaat cagggatgaa taattgcgat taccgccttt tcccagttcg
     9901 cgacatagaa tccttttcat tcattcttaa tgatattcct catttatatg gactaaatgt
     9961 taccataccc tataaagagg ctattatacc atggttgaac gaattatcgc ccgaagccaa
    10021 actgattgga gcggtaaatg tcatcaagat aattcataaa ggaactaaaa tacagacgat
    10081 agggcataat acagatgcgg cggcatttat tcgaacactt ctgccactgt taagaagtca
    10141 tcatagtcag gcaatgatat ttggatgtgg aggtgccaca aaagctgttg catatagctt
    10201 gagtttgcta ggtattccat atatcatcgt atcgaggggg aaaaagggtc cgcatatcat
    10261 ttcgtatgaa gagctcagca tttcattgat taataaatat cctgttttga ttaattgtac
    10321 acctgtgggg atgttttctt accccgatga agctctctcc attcctttcg aagggatagg
    10381 tcctcgtcat ctggtatacg atttgaccta caatccacct ttgactcaat tgctgaaaat
    10441 tgctcagcaa aggggtgcta caacggcaaa tggattacaa atgttgcact atcaggcaga
    10501 tttgtcgtgg gaattttgga acgatcggtg aaaagattaa ttcacccgaa ggacttgacc
    10561 tggtttaatg atattttttg gtcgaatatt attgagcctg caaattgcag ctatggtaat
    10621 tccatatttt tgggcaattt tacttaaggt atcacctttc ttcactgtat gaaaaatttc
    10681 ttctgaaccg acggattcta cctgtggcct tgatttagct gtcaagcgtg tgcgtgtagt
    10741 tagaaattca tcgtccaaat atatggatga tgcttttagt tctcctgttt cgaaacagat
    10801 tattttttcg ggatcgaaag ccttgtcgaa atatcggact tcgaaatgca gatgagagcc
//
LOCUS       DAOORU010000074        10858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7571_length_10858_cov_5.434694, whole genome shotgun sequence.
ACCESSION   DAOORU010000074 DAOORU010000000
VERSION     DAOORU010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10858
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7571_length_10858_cov_5.434694"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLF20_05990"
     CDS             <1..281
                     /locus_tag="PLF20_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80587.1"
                     /translation="YQSLLIPELWLIKKWVDMRNVPKWCWTTMHASWVHQKVDYFGIR
                     VQKEDVYHYGWIKTYKTYTNNTTNRIDSIVYCRLPNKPFTVGHFDIDI"
     gene            complement(651..2552)
                     /locus_tag="PLF20_05995"
     CDS             complement(651..2552)
                     /locus_tag="PLF20_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOX80588.1"
                     /translation="MVHRFSLKTFSVKFLIFSVFQISSLAVFAHQDSINFIPAIPVDI
                     QPNFITIEWQTSNDVFSGVYYEAEGKNAWILDVATKPLRNHSYNIPASYPSQVIYALP
                     FQFVGSDTLWGDTLVLMSQSASSGKMRVYFTRPIDPNVATGSQAIYLPGTAADTVIEL
                     IHRAKKSIDLAIYNMNIDGIAYALNQAKVRGVRVRVIYDGSTTNSSIYVLQGVPILAS
                     PTSSSYGIMHNKFMVIDCDTDDPNDAILFTGSMNFTSTQVNKDANNILIIQDRSLALA
                     YRMEFEEMWGGSGNQPNPSASRFGPFKKNNTTHYFNVGGIPIESWFSPSDGVNSKILA
                     AINSANTSLFIPTNLITRDDLANAIEKRRQNGVLCRVVVDDDASCSDQVVATLTQALG
                     TNFRETTESGILHHKVLIADALQTNSNPVVLTGSHNWSNNAEIRNDENTLILYDASIA
                     NLYYQEFSQRFKQSRVIAPNPMLDLGPDISVCAGQKVILDAGTGFFSYLWSNGSTASQ
                     IIVDTSGMGLGSITLSCTVNNVYGTQTDQITITFFTCTSIEDNLTKRQFSIYPNPTRE
                     FFRMQLPPDVKPQFIEVYGIQGQMVMRQTTRLVGDSLEINVSELNAGIYMVRVLAGNK
                     VYVARLLKQ"
     gene            complement(2580..3617)
                     /locus_tag="PLF20_06000"
     CDS             complement(2580..3617)
                     /locus_tag="PLF20_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOX80589.1"
                     /translation="MRKSFFVIQAFISFLFIGIITLQAQQTQGKKKAVCIAFYNTENL
                     FDTIDDPLTDDKEFLPDGANQWNTSKYMEKLNHLAEVISQIGDDYVKGGPTVLGLSEI
                     ENRRVLEDLVKMPELSKQGYAIIHFDSPDNRGVDVALIYKYHVFKVSSARAVPLHIPD
                     MPNFKTRDQLVVSGMIDGEMLHFIVNHWPSRFHGPEYRAAAASLTRHLVDSLQNLYPQ
                     AKVIIMGDLNDDPTDPSVLTVLNAKGDKREVQPGDLYNPMWKLFKNGVGSLAYRDNWN
                     LFDQIILTYPLINQENNGFKFYTARVFNKKFLFNKEGQYKGYPFRTFAGGAYQGGYSD
                     HLPVYIILIKE"
     gene            3899..6595
                     /locus_tag="PLF20_06005"
     CDS             3899..6595
                     /locus_tag="PLF20_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX80590.1"
                     /translation="MKRFILTLCLGFCLLSLKAQQIQLKGILVDETTQSPIANAIIKI
                     EDKTISTDSEGFFSLSELKPGRLGLWIEATGYEPYSMVVIFTGEAMDLGKISLRPFIL
                     SAIDNGLAEIALVAEGSDDESEIQAGSALLAASSDIFSSIAAYTFSPMYFKTRGYDNE
                     NIAVYMNGLMVNDPENGRPSWSEWGGLNDVTRNKEISNGMQFSQNLLGSLGGSTNIIT
                     RPSLQRKQTKFSYSLSNRTYVHRLMFTYSTGMRPDGWAISFSGSRRWGNEGYVEGIFY
                     DAWAYFLGIERKINNNHSLALTVYGSPTRRGQQGPATQEAYDLSETNFYNPYWGYQQG
                     DKRNSRVKDFHEPMILLNHYGKLSENLQWNNGLAVSFGYNGGTALNWYNAPDPRPDYY
                     RYLPSYATDENIRKMVADRWKSSENVRQINWDKLYQINYLSNLEGKGARYIVENRRND
                     QLQFLINSTLNYQINQNIALSGGATYRNYRGSHFKLIEDLLGSNYWLDIDQFAERDFT
                     GDTIKAQNDLNNPNRKVSEDEKFGYNYCVYQQLLQAWVQTEFSYDAGNFYAGIAYTGQ
                     QFYREGLMRNGRHPDNSFGDSQKYNFNDATLKTGGTYQIFPRNFIQVNGMYMTRAPFI
                     QNVFISPRTRADVLPQMVSEKIVSGEASYLMRYPFLNLRFTVFHTIFDDQSEVNSFYH
                     DDLKTYVNYVLTGISKTHQGLEFGTEVKITSALSLQAVAAWGNYRYTNRPLATICYDN
                     GSKPDTTERVYIKNFYVPGTPQTAASLGLKYAGRRYLYINLNANYFDRNYVDFNPERR
                     TSAAIANLGPGDPLITTITQQEKLPDAFTLDASIGKSWRWGEYFLNLNFSVSNILDNK
                     KVITTGFEQMRFDFTNKDLAKFPPKYYYGYGRNFFLNLGFRF"
     gene            6636..8525
                     /locus_tag="PLF20_06010"
     CDS             6636..8525
                     /locus_tag="PLF20_06010"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5689 domain-containing protein"
                     /protein_id="HOX80591.1"
                     /translation="MNFSNTKLFFFIGLMAVAAVFTGCVKGDFDEPPINIPTVDFEAN
                     TTIAQLKASYTGILDSISDDIIIKGVVVANDESGNLYKQLVIQDETGGIMLLIDKSYL
                     YTEYKLGQRIYVKCKGMYLGDYNKLIQLGYIYNNAIGRLPEVMVEDHIFRDSLPGKVP
                     APVNIDVANNNSAYINMLVRIENVHFLEVGVPFAPQGASATNRTIADASGNELIVRTS
                     QYANFASMLTPSGYGHLQGILTIYQTTYQLLIRDTADIIGFSGSTPPPPPSGDYVFPD
                     PNFPTISSLEENFDGITNQTDISISGWTNEATKGNRKWQGKIYQTEYYAQATSYNSTD
                     AENVTWIVTPPVIYSNNLILSFKSAMAYYKHDGLSVWILTNYTGNPETATWTQINATL
                     ANSSSGDNNWVASGDIPLSNFVPTNYTGNIYIGFRYEGTQTNTTTYRIDEVKINTTGG
                     GGGGGGTVTPVSEIHEMFDNVQNDVNIEIEGWLNLAETGTRFWRGKIFNTEKYAQATS
                     YKTLDNQNICWLITPPVMFEGGKLLNFKSAKAYWVHDGLSVWISTDFNGSNLTTATWT
                     PINATLAGKANADHEWVSSGNISISNYVPQGFTGNFYIAFKYIGDAVNTTTYRIDEVN
                     IEPVK"
     gene            complement(8976..9401)
                     /locus_tag="PLF20_06015"
     CDS             complement(8976..9401)
                     /locus_tag="PLF20_06015"
                     /inference="COORDINATES: protein motif:HMM:NF019468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HOX80592.1"
                     /translation="MAILLIWIFVSINYTSMPATIPVHYNANGQADSFGKKSNILLLT
                     AITTVFFVGLTVLNRFPHIFNYPTPINSQNARRQYTNATRMIRYLKLILVLIFGSIIL
                     LTIQYTKGKSEGLGIWFLSLMSVLIYIPLFYFIARSLRK"
     gene            complement(9637..10662)
                     /locus_tag="PLF20_06020"
     CDS             complement(9637..10662)
                     /locus_tag="PLF20_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80593.1"
                     /translation="MGNAIPTIQDWKNLFDAALKFKKIAPWQWMWDSDIFGVQNPANG
                     EIGYCCIMGAAKEIYALAVYQGSDGLESYLRLNTKYHSESLFDLYTLQKCVMLSFEDR
                     ANLTNEDLQIIKSLGLQFRGHNEWPLFRSFRPGFFPWFIAKEEAEFLTVAIHQAIEVA
                     LQFEKNPNLLSSIDEDYYLVRVPHIENDAIVWKNELLKTQPYKEFEIKTKPVDEKLLE
                     RINKLHILQKDTWEIDYFFYPSAVKEKNERPFYPYIILWVDHRSGYILHHHLANDKQE
                     LAKIPELFLHFIEYFNSIPKTIITKHEMTYQLLEPVAKSLNINLIKKEKLKGIEEAQI
                     AFFENLR"
BASE COUNT         3371 a         2171 c         2061 g         3255 t
ORIGIN      
        1 tttatcaatc attattaatt ccagaattat ggttgataaa aaagtgggta gatatgcgta
       61 acgtaccgaa atggtgttgg actactatgc atgcatcatg ggtacatcaa aaggtggatt
      121 attttggcat aagagttcaa aaggaagatg tctatcatta tgggtggata aaaacatata
      181 aaacttacac taataatact accaaccgca tagattccat agtttactgt cgtttaccca
      241 acaagccatt tacggtaggg catttcgata ttgatatcta agaatcagtt tcttattgcc
      301 ttaacatcag aactcttgat cttttcattg catgttaata tttttataga acagaattat
      361 attttaaatt atcttatttt ctgtcaaaaa ttttcaaata tataaaccta acaggtctta
      421 gagacttgtt aggttttact aaaagatatt acaaggtgag gaaaggaaac tcaaaaaatt
      481 aaaggtgctg aatgaaggga atgcacttca acaacgtaac gaaattgatc ctacagagta
      541 gaaaatacta tatatcaaac tttaaaaacc tgataactat attaaatgcc gaatcaagag
      601 ttctgcaaca ctcgaattct ttcacgaata acactatctt cttagaatga ttactgtttc
      661 aataggcgag ccacataaac tttattgccg gccagcactc ttaccatata aattccggca
      721 ttcagctcac taacatttat ttctaaagaa tctccaacga gtcgggtggt ttgccgcatt
      781 accatttgtc cttgaatgcc gtaaacttca ataaattgtg gtttaacgtc aggcggtaac
      841 tgcattctga aaaattcccg ggtgggattg ggataaatag aaaattgtct tttcgtaaga
      901 ttatcttcta tgcttgtaca tgtaaaaaag gtaatggtta tttgatcagt ttgcgtacca
      961 tagacattgt taacagtgca gctcaatgta atgcttccca gacccattcc ggaagtatcc
     1021 acaattatct ggcttgccgt gctgccattc gaccacaaat aggaaaaaaa tcctgttccc
     1081 gcatccaata ttactttctg gcccgcacaa acgcttatat cgggtcctaa gtcaagcatg
     1141 gggtttgggg ctatcactct cgactgttta aaacgctgag agaattcctg gtagtataga
     1201 ttggcaatgc tggcatcata aagtataagg gtattttcgt cgtttctaat ttcggcatta
     1261 ttgctccaat tatgagagcc ggtgagtacc acaggattgg agttggtttg tagggcatca
     1321 gcaatcaaga ctttatggtg aaggatacca ctttcagtag tttctctaaa atttgttccc
     1381 aaagcctgag taagtgtagc caccacttga tccgaacatg aggcatcatc atctaccacc
     1441 actcgacaaa gtactccatt ttgtcttctc ttctcgatag cattggcaag atcatcacga
     1501 gtaattaaat tggtaggaat aaataaactg gtattagcgc tgttgatagc tgcaaggatt
     1561 tttgaattta ctccatcgga agggctaaac cagctttcga ttggaattcc acccacatta
     1621 aaataatgag tagtattatt tttcttaaaa ggtccaaaac gcgaggcaga tggattaggt
     1681 tggttgccag agcctcccca catttcttca aactccatgc gataagctaa tgccaaactt
     1741 ctgtcctgaa taatcaaaat attattggca tccttgttaa cctgagttga agtaaagttc
     1801 atacttcctg taaaaagaat tgcatcgtta ggatcgtctg tatcgcaatc gataaccatg
     1861 aatttattgt gcatgattcc gtaagaggat gaagtagggc tagcaagtat gggcacgcct
     1921 tgaagtacat aaatcgagct gttggttgtg cttccgtcgt aaatcactct tactctcacc
     1981 cctcgtactt ttgcctgatt caaggcataa gcaatgccat cgatattcat attgtaaata
     2041 gccagatcta tacttttttt ggctcgatga atgagctcga ttaccgtatc ggcagccgtg
     2101 ccgggcagat atatggcttg agacccagtg gcaacattgg gatcgatagg acgagtaaaa
     2161 taaaccctca tcttccccga agaagccgac tgagacatca gcaccaatgt atcaccccaa
     2221 agcgtgtcac ttccaacgaa ttgaaaagga agagcataaa tcacttgcga aggataagac
     2281 gccggaatat tgtaactatg attccgcaga ggtttagttg caacatccaa aatccaagca
     2341 ttcttgcctt ctgcttcata ataaacccct gaaaaaacat cattcgaagt ttgccattca
     2401 atagtgataa aattgggttg aatatctaca ggtattgcag gaataaaatt tatactgtct
     2461 tgatgggcaa aaacagctaa tgaagatatc tgaaaaacag aaaaaattag aaattttact
     2521 gaaaaggttt ttaaagagaa acgatgcacc attaggaaaa aattttaggt tgaaaaactt
     2581 tattctttga tgaggataat ataaaccggt aaatgatcag aataaccgcc ttgataggca
     2641 ccaccggcaa aggtgcgaaa aggatagcct ttatactgtc cttctttatt aaataaaaac
     2701 tttttattga agactcgagc cgtataaaat ttaaaaccat tattttcttg atttatcaaa
     2761 ggataagtga gaatgatctg atcgaacaga ttccagttat cacgataagc caatgatcct
     2821 actccatttt tgaataattt ccacatggga ttgtataaat cgccaggttg aacttctctt
     2881 ttgtctcctt ttgcattcag taccgttaaa acgctaggat cggtgggatc atcgtttaaa
     2941 tcgcccataa tgatgacttt tgcttgtgga tagagatttt ggagtgaatc gaccagatga
     3001 cgcgttaaag aggcggctgc agctctatat tctggaccat gaaaccgtga gggccaatga
     3061 tttacgataa aatgtaacat ttctccatct atcattcccg aaacaaccaa ctgatcacga
     3121 gttttgaaat tgggcatgtc gggaatatgc aagggaacag cccgtgcaga gctaactttg
     3181 aatacatgat atttgtaaat taaagccaca tcaactcccc tattatctgg tgagtcgaaa
     3241 tggataatgg cataaccctg ttttgaaagc tctggcattt tgaccaaatc ttcaagtaca
     3301 cgacggtttt ctatctccga taaacccaaa acagtaggac caccttttac ataatcatct
     3361 cctatttgag aaattacttc ggcaagatga ttcaatttct ccatgtattt cgatgtattc
     3421 cactggttgg caccatcggg taaaaattcc ttatcgtcag taaggggatc atcaatagta
     3481 tcgaaaagat tttcagtatt atagaaggct atacacactg cttttttctt cccttgggtt
     3541 tgctgagctt gtaatgtaat tatcccaata aataggaatg agataaaagc ctgaatcaca
     3601 aaaaaggact tacgcatttg ttataataat aatttgcgcc aaaaatatca ataatttaga
     3661 tgagtagatt tatttaatgt ctttgcaata agttgatttc tgccaatgtg gcttatattt
     3721 cgaaacccga cgaatatcgt catttttcga gctaaattac ggatatattt tatcattata
     3781 aaaaactcac cgcaaaaacc ttctctcttt caccaatatt tccatatttt tcgttccttt
     3841 gtaaccccaa atttcgaaca ttcgattgat gggaaagcat aatgtaaact cactaaggat
     3901 gaaaagattc atacttacac tttgcttggg attttgttta ctttcactca aggcacagca
     3961 aatacaacta aaaggcattc tcgttgatga aaccacacaa tctcccattg ctaatgcaat
     4021 tattaagatt gaggacaaaa ccattagcac ggattcagaa ggatttttta gtttaagtga
     4081 acttaaaccc ggacgattag gattatggat agaagccacg ggatacgaac catattctat
     4141 ggtagtaatt tttacgggtg aagccatgga tttgggaaag atttctttac gtccatttat
     4201 tctatctgcc atcgacaatg gtttggctga aattgcgctg gttgccgaag gtagtgatga
     4261 tgaatcggag attcaggctg gctcggcttt acttgcggct tcttccgata ttttttcctc
     4321 tattgcggct tataccttca gcccaatgta ttttaaaaca cgaggctacg ataacgaaaa
     4381 tattgcggtt tacatgaatg gactgatggt gaatgatcca gaaaatggac ggccatcgtg
     4441 gtcggaatgg ggagggctca atgatgttac ccgcaataag gaaatcagca atggcatgca
     4501 attttcccaa aatcttctcg gttctttggg tggaagtacc aatatcatta cacgcccatc
     4561 acttcagcgc aaacaaacca aattcagcta ctccttatcc aaccggactt atgttcatcg
     4621 attgatgttt acttacagca cagggatgcg tccggatggc tgggctatca gtttcagtgg
     4681 ctcacgccgc tggggaaacg aaggttatgt agaaggaata ttttacgacg catgggctta
     4741 ttttttaggc atagaacgca aaatcaacaa taaccatagt ttagccttaa cagtttatgg
     4801 ctcacctacc cgccgtggac agcaaggtcc agctactcag gaggcttatg acctgtcaga
     4861 aactaacttt tacaacccct attggggcta ccaacaagga gataaacgaa attctcgagt
     4921 aaaagatttc catgaaccca tgatcctcct caatcattat ggaaaactca gcgaaaacct
     4981 acaatggaac aacggtctgg ccgtaagctt tggatataat ggaggaacag ccttgaattg
     5041 gtacaacgct cccgatccac gtcccgatta ttacaggtac ttacccagtt atgccaccga
     5101 cgaaaatatc cgcaagatgg ttgccgaccg ttggaaaagt tcggaaaatg tccgccaaat
     5161 caattgggat aaactttatc aaatcaacta tttgagcaat ttagaaggga aaggagctcg
     5221 ttatatcgtg gagaaccgtc gcaacgatca gcttcaattc ttgatcaatt caactctcaa
     5281 ttatcaaatc aaccaaaaca tcgcactttc tggaggagct acttacagaa attaccgtgg
     5341 gtctcatttt aaactcatag aagatttgct gggatcgaat tactggctcg atattgatca
     5401 gtttgccgaa agggacttca cgggtgatac catcaaggct caaaatgatc tgaataatcc
     5461 caatcggaaa gtttccgaag atgaaaaatt tggatataac tactgtgtct atcaacaatt
     5521 gttacaagcc tgggtgcaga ccgaattttc gtatgatgca ggcaattttt atgcaggtat
     5581 tgcatacaca gggcaacaat tctatcgcga gggcctgatg cgtaatggcc gccaccccga
     5641 taattccttt ggcgattctc aaaaatacaa tttcaatgac gctacattaa aaacgggtgg
     5701 cacatatcaa atctttcccc gtaatttcat tcaggtaaac ggaatgtata tgacccgggc
     5761 accctttatc cagaatgttt tcatttcacc acgtactcga gccgatgtac tgccccaaat
     5821 ggttagcgaa aaaatcgtga gcggcgaagc cagttacttg atgcgttacc cctttttgaa
     5881 cctacgtttt acggtttttc acaccatttt cgacgaccag agcgaagtaa acagttttta
     5941 ccacgatgat ttaaaaactt atgtcaatta tgttctcaca ggaataagca agactcacca
     6001 aggattagag ttcggaacag aagtgaagat tacttcggct ttgtcgctgc aagcggttgc
     6061 agcttgggga aactatagat ataccaatcg tcctttagcc actatttgtt atgataatgg
     6121 ctcgaaaccc gataccaccg aaagggtata tatcaagaat ttctatgtcc cgggaacacc
     6181 acaaacggct gcctctttag gtttaaaata tgctggtcga cgttatcttt acatcaacct
     6241 caacgccaat tatttcgacc gtaattatgt agatttcaat cccgaacgcc gtacttcggc
     6301 ggccattgcc aaccttggcc ccggcgatcc cctcattacc actatcactc aacaggaaaa
     6361 actgcccgat gccttcaccc tcgatgcttc aatcggaaaa tcatggagat ggggagaata
     6421 ttttctgaac ctcaacttct ccgttagcaa tatactcgac aacaaaaagg tgattactac
     6481 cggattcgag caaatgcgct tcgacttcac taacaaagac ttagccaaat tcccacccaa
     6541 atattattat ggatatggaa gaaacttctt cctaaatctc ggttttcgtt tttaaattcc
     6601 tcatccaaaa gaaaaatacc aaaacattat aaagtatgaa tttctcaaac accaagcttt
     6661 tcttttttat tggccttatg gcagttgctg cagtatttac aggctgcgtc aaaggagatt
     6721 tcgacgaacc tcctatcaac attcctactg tcgattttga agccaatacg actattgcgc
     6781 agcttaaagc ctcatatact ggaattctcg attctatcag cgacgacatc attattaaag
     6841 gagtcgtggt tgccaatgat gaatcgggaa acttatacaa acaactggtc attcaggatg
     6901 aaactggagg cattatgctc cttatcgata aatcctatct ttataccgaa tacaagctcg
     6961 gacagagaat ttacgtcaaa tgcaagggaa tgtatttggg tgattacaat aaactcattc
     7021 agctgggcta tatatataac aacgctattg gccgactacc cgaagtgatg gttgaagacc
     7081 atattttccg tgatagttta ccaggaaaag ttcctgctcc agtcaatata gatgtggcta
     7141 acaacaactc agcctatatc aatatgctgg taagaataga aaacgttcat tttctcgaag
     7201 tcggagttcc ctttgcaccc caaggagcat cggcaaccaa tcgcaccatt gctgatgcct
     7261 cagggaatga acttatcgta agaacaagcc aatatgccaa ttttgcaagt atgcttactc
     7321 cttcgggcta tggccattta caaggtatac ttacaattta tcaaaccacc tatcaacttt
     7381 taattcgtga tacagccgac attataggtt tttcaggctc tactcctcct cctccacctt
     7441 ctggcgatta tgtctttccc gatcccaatt ttcccaccat ttcttcactc gaagaaaatt
     7501 tcgacggaat aaccaatcaa actgatatct ccatttcagg ttggactaac gaagccacaa
     7561 aaggtaaccg caaatggcag ggaaaaattt accaaacaga gtattacgcc caagctactt
     7621 cctacaattc aaccgatgca gaaaatgtaa cctggatcgt cactcctcct gtcatttatt
     7681 ccaataatct gatcttgagt tttaaaagcg caatggctta ttacaaacat gatgggctca
     7741 gtgtatggat ccttacaaat tataccggaa atcccgaaac tgccacttgg actcaaatta
     7801 atgctacatt ggccaatagc tcctcgggcg acaacaactg ggttgcctca ggtgatattc
     7861 ctctttccaa cttcgttcca acgaattata cgggtaatat ttatattgga ttccgttatg
     7921 aagggaccca aaccaatacg actacctatc gtattgatga agtaaaaatc aacacgaccg
     7981 gaggcggtgg tggaggagga ggtacggtta cccccgttag cgaaatacac gaaatgttcg
     8041 ataatgttca aaatgacgta aacattgaaa tcgaaggttg gctaaatctt gccgaaacag
     8101 gcacacgttt ctggagagga aaaattttca ataccgagaa atatgctcaa gccacctcat
     8161 acaaaaccct cgacaaccaa aatatatgct ggttaatcac tcctccagtg atgtttgaag
     8221 gaggcaaact tctcaacttt aaatctgcca aagcttactg ggtacacgat ggcttaagcg
     8281 tatggatttc gaccgatttt aatggctcta acctcacaac cgctacatgg acacccatca
     8341 atgcaactct cgccggcaaa gccaatgccg accatgaatg ggtttcttcg ggaaacatat
     8401 ctatcagtaa ttatgtccct cagggattta ccggaaactt ctatattgct ttcaaatata
     8461 ttggtgatgc tgtaaataca accacttatc gcatcgatga agtaaatatc gagccagtaa
     8521 aatagagcat tctcattatt tttctttttt ttatttttag cggggcaatc catttcgtat
     8581 agctccggtt ttttatatgt gaggcggaat agtgtcgtga gatgtatttg agtggaaagt
     8641 aaaagaatta cgttgtgcat attatgtggt ctggattgcg gagaatggat aatgtgagtt
     8701 taaagttaaa agttcataat ctttatgaag ggaaacagaa aatgggaaaa gggagaatag
     8761 ggcatagtat cttttgaacg gagtcaacat ctgccgaaat gtcatctgaa agtcggtcaa
     8821 taaacatatt cttttttcct ctgcgaggag ttaatgtgct caaaaataat cactcaatga
     8881 ttcggagttt ttcttctttg gcaaattttt ccttttcaca ataatgcaat tatgattttt
     8941 tttcgagggt caagttattg cattaaagaa ttgtttcatt ttctgagcga tcgagcaata
     9001 aaatagaata aaggaatgta tatcaagact gacattaaag ataaaaacca gattccgagt
     9061 ccttcagact tgccttttgt atattgtatg gtaaggagaa taatcgaacc aaaaatgagg
     9121 acgaggatga gcttcagata acgtatcatt cttgtagcat ttgtatattg ccgcctggca
     9181 ttttggctgt ttatcggggt gggatagttg aatatatgag ggaatcggtt taagacggtc
     9241 aaccctacga aaaaaactgt agtaatagca gtcagaagaa gaatattgga ttttttacca
     9301 aaactatcgg cctggccatt tgcattataa tgaacaggaa tagtggccgg catgctggta
     9361 taattgatcg aaacaaaaat ccagataagc agaatagcca cccatcctgt ggtttcgaga
     9421 aatttgtcgg ttactcccca cccgagtttt attcttggcc tttactcagc ttttgagcta
     9481 tttttagaat gattcattgt tattgaattg tgcaagctct tttcggaagc atgaccctac
     9541 ggattttata tttgagttct gtactcacaa aatcctttct tgctcaaaat taaattagta
     9601 tcctgaatat cactcgaaga aattgagaat tcgattttat ctcaaattct cgaagaatgc
     9661 aatttgggct tcttcaattc cttttaattt ttctttcttg ataagattga tatttaaact
     9721 tttggcaact ggttcgagca attgataggt catttcatgt ttcgtaatga ttgtcttagg
     9781 tatagaattg aaatattcta taaaatgaag gaataattca ggaattttag caagttcctg
     9841 tttgtcgttc gccaaatggt gatgcaagat ataaccggaa cgatgatcta cccaaagaat
     9901 gatataggga tagaaaggtc tttcattttt ttcttttact gccgatggat aaaagaaata
     9961 atcgatttcc catgtatctt tttgaaggat gtgtaattta ttgattcttt ccagtaattt
    10021 ttcatctaca ggtttggtct taatttcgaa ttccttgtat ggttgcgttt tcagcaattc
    10081 atttttccat acgatagcat cgttctcgat gtggggaact cttaccagat aataatcttc
    10141 gtcgatagaa gaaagcagat taggattttt ttcgaattgc aaggcaacct caatagcctg
    10201 atggatggcc acagtcaaaa attctgcttc ttccttggca atgaaccatg ggaaaaaacc
    10261 aggccgaaaa cttctgaaca aaggccattc gttatgtcct ctaaactgta aacctaagct
    10321 tttgataatt tgaaggtctt cattcgttag atttgctctg tcttcgaaac taagcatcac
    10381 gcatttttga agagtatata gatcgaaaag cgattcggaa tgatattttg tattcagcct
    10441 cagataactc tccagaccat ccgaaccctg atatacggcc aaagcataaa tctctttagc
    10501 tgcccccatg atacagcaat aaccaatttc tccatttgca ggattttgta ctccaaatat
    10561 atccgagtcc cacatccatt gccaaggtgc aatttttttg aatttcaaag cagcatcgaa
    10621 caaatttttc cagtcttgaa tagtaggtat tgcgtttccc attttgaacc tcacatttta
    10681 ttgatgttat ttgattttat atgaacaatg gttggccaag caataaattt aattttctat
    10741 atctgttcag acaagcgatt gccttagtat atttattgtg atcatctgta tttttatttc
    10801 atcactgacc gacaaaaaaa ttggataaaa agaaggaggg acaaaaccga agtttcat
//
LOCUS       DAOORU010000075        10840 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7588_length_10840_cov_4.732777, whole genome shotgun sequence.
ACCESSION   DAOORU010000075 DAOORU010000000
VERSION     DAOORU010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10840
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7588_length_10840_cov_4.732777"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            243..1046
                     /locus_tag="PLF20_06025"
     CDS             243..1046
                     /locus_tag="PLF20_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80594.1"
                     /translation="MLSVCSFSEQAIIDFCEVTLDHNVVHDPAYMKSQSKKAVVPGML
                     ILSKAIGLAEEIIYKGANTFQLYLNSMVGANEFLTIGYNMIAETPVKAEIVARNSTDA
                     LRHRGNPSTIFKRDHDPQFEYLGNRRHLETDAAQVESFSEIVGLKGKPLGALLFSIAY
                     ASHSLFRAIEKPVSRMEREIREYLSKDARNDKQSPFYQSISIHLPDNIYVPQGGFPLD
                     FLIHFERIKEGRTYSAQVICTQNDRDIYFSKYVLTAIPDRLIMRMAPNL"
     gene            complement(1062..1673)
                     /locus_tag="PLF20_06030"
     CDS             complement(1062..1673)
                     /locus_tag="PLF20_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOX80595.1"
                     /translation="MKIICIGRNYKAHVEELHNRLPENPIFFMKPETSLLPRHHPFYF
                     PEFTKELHYECELVLKICRLGKHIDERFALGYVESIGIGIDFTARDLQRKCKEEGLPW
                     EIAKAWDFSAPLGAFIRKEEFEDLKNISFSLQKNGETIQQGNSRDMIFSFSQIITYVS
                     KFITLKQGDIIFTGTPEGVGPVKSGDLLEGYIFDQKCLKLKIM"
     gene            1870..2784
                     /gene="xerD"
                     /locus_tag="PLF20_06035"
     CDS             1870..2784
                     /gene="xerD"
                     /locus_tag="PLF20_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOX80596.1"
                     /translation="MQTQWNEWIKGFRAYLTLEKGLSANTLEAYVHDVQLLTEYFEIT
                     SIVKSPAEVSREDLSRFVEYIYQLGLEASSQARIISGIRAFFKYMLLEGTLNENPAEL
                     LEIPRLQRKLPVVLSEEEIDRLIRIIDVSRPEGQRNKAIIETLYACGLRVSELIELRI
                     SRLYLEEGYIRVIGKGNKERFVPIGEEAIHNIVLYIENVRNTLKVQKQWSDIVFLNRR
                     GGKMSRVMVFLIIKDLAAKAGISKKISPHTFRHSFATHLVENGASLRAVQDMLGHASI
                     TTTEIYTHLDKEYLRRTLLDFHPFYVKK"
     gene            complement(2893..3669)
                     /locus_tag="PLF20_06040"
     CDS             complement(2893..3669)
                     /locus_tag="PLF20_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80597.1"
                     /translation="MKTLILSDQEASELQEFYMRELARAQRRLSHCQQILSKFESPVE
                     ITEIKPKQVTATNEPIAGEVYTEKKKRGRPRKDQQTAGQAIAAPEAALAAPKKIGRPR
                     KEKPVLEKGETPETEVSKNIPQPFETESLAQEMPTEVVEENIEIQTSKKLKKAGKKAD
                     KVETKSSKKTIKQSEELGIKIPWTSFIMDTLKNATHPLTAKEIANIAIKQYEIPDKKT
                     NYVRNTIAACLSGLATRSKKIDAIATEAKVKAYLLPPSEN"
     gene            complement(3872..4414)
                     /locus_tag="PLF20_06045"
     CDS             complement(3872..4414)
                     /locus_tag="PLF20_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX80598.1"
                     /translation="MIPRIIIKLNKFTLRPFSWADTTSLANYANDSRVATFLRPSFPY
                     PYTSKDAEEYIYFCLNAQDAFVLAIDINHEAVGSIGVQYIQFVGKSTWELGYWLGVPF
                     WNQGIMSDAVKNFIHYLAFYLNTDEIIAKVYSLNIGSIKVLENNGFKKLNFNETTRIR
                     TGQLVEEWVFMKNLERHIFR"
     gene            complement(4411..5526)
                     /locus_tag="PLF20_06050"
     CDS             complement(4411..5526)
                     /locus_tag="PLF20_06050"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HOX80599.1"
                     /translation="MMKKLYQVSRLLLGMTFIFSGFVKGQDPLGTMFKIEDYLIAYGW
                     DWALPFALALSILLCTAEFVIGAGLILNYQTKLIQWLILLFMVFFTCITFYDALYNAV
                     SDCGCFGDAIKLTNWQTFFKNIVLLALVAILFYTSRNAKPPFFRRTALFIAVTCIFAG
                     FSIWSFYHLPLIDFLPWKKGNIVSTVSQQSSEYYVTYRNKTTGEEREFPSNDFPYKDS
                     IWMSQWEFVSSRVVSHPSSDNTLLLISDTLGNDVSLYYLNYPDFLFIATIYDLHSAPD
                     KSLKKLSDFIRSAENSGYTCIILTSALNKEIEDFALKYDIQAEIFNIDDITLKMMVRA
                     NPGLILIKNGVIIDKWNVRSMPDFNKIEKKHLKLTNE"
     gene            complement(5549..6094)
                     /locus_tag="PLF20_06055"
     CDS             complement(5549..6094)
                     /locus_tag="PLF20_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1599 domain-containing protein"
                     /protein_id="HOX80600.1"
                     /translation="MQNTLLQYDEAVAECRKVFEAKLKDYGPSWRVLRLPSITDQIYI
                     KARRIRTLQSSSEQRINEGIKPEFIGIYNYSLIALIQHELGPEPENASPSNIDYLNSY
                     NRWSQKAKQLMLDKNHDYDEAWRQMRLSSLTDIILMKIKRIKQIEDNSGLTLVSEGIE
                     SNYLDIMNYAIFSLIKISENK"
     gene            6130..7008
                     /gene="folP"
                     /locus_tag="PLF20_06060"
     CDS             6130..7008
                     /gene="folP"
                     /locus_tag="PLF20_06060"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOX80601.1"
                     /translation="MNLGSKDTNSLTTRGLLIGEKGIMIPFPAVMGILNITQDSFYDG
                     GRYLTEDQWINRTALMLQEGASIIDVGAASTRPGAQEIPEIFEIEKIRKVVHTLIQHF
                     PDIIISVDTYRSLVAQTALDEGASIINDISAGDFDASMIPLVASRKVPYIMMHILGTP
                     ATMQINPHYNDVVTDIIDYFKHRIEVCNRYGLDSLILDPGFGFGKTLKHNYTLLSHLS
                     SFIKLGYPVLVGVSRKAMIYKLLNVQPEEALFGTVVANALALLNGAHIIRVHDVKPTI
                     QAIKILDEYKDSLTNP"
     gene            7183..8022
                     /gene="cdaA"
                     /locus_tag="PLF20_06065"
     CDS             7183..8022
                     /gene="cdaA"
                     /locus_tag="PLF20_06065"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOX80602.1"
                     /translation="MLIKTILPLFITFRWIDAIDIFLVALLLYEFYNLVKGTGAINIF
                     IGIVAVIILWKIVSALEMVLLSEILGAFISVGFIALIVVFQPEIRQFLLLLGNPSFIQ
                     ERRRKFFFWRIPRANDERLAIDNVVSACQRMGNSATGALIVIAKTNELKEYVQSGEII
                     DSLISVPLLETIFFKNTPLHDGAAIIVNNRIKSARCILPVSSNNKIPIDLGLRHRAAI
                     GITERTDAIALIVSEETGEISVAKEGTLVQNIRPAQVKDFLEKEFAPHEAAPRKRKVS
                     KKE"
     gene            complement(8037..8588)
                     /locus_tag="PLF20_06070"
     CDS             complement(8037..8588)
                     /locus_tag="PLF20_06070"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX80603.1"
                     /translation="MNNKNIYVLSLALIVVVVIWGCSNKQEHLKNEITNIEKQLFNDT
                     TGIIMPQKAQKTIELYSEYIHNYPHDSLSAEYMFRAAQLCTAIGNFEEAIKFYDSLII
                     SFPEIPKAAEAMFMKAFVFDNYLYRYDKARENYMLFIQKFPNHDLRRDAEKSIQFLGM
                     SNAEILEILQADADKNNLEKNQQ"
     gene            complement(8607..9179)
                     /gene="def"
                     /locus_tag="PLF20_06075"
     CDS             complement(8607..9179)
                     /gene="def"
                     /locus_tag="PLF20_06075"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOX80604.1"
                     /translation="MIYPIVAYGHPILRQKAADIKSDFPDLQEFIKNLWETMYATDGI
                     GLAAPQVNRAIRIFVIDATVLAKNYPETTGFKKTFINAYKINEGGDAWLFNEGCLSIP
                     DIHEDVSRPKRILLHYLDENFDEHEEWFEGAVARTIQHEYDHLEGILFTDYLSPMRKT
                     MLARKLNNIALGNIETKYRMIFPRSKKQKR"
     gene            complement(9192..9620)
                     /gene="ruvX"
                     /locus_tag="PLF20_06080"
     CDS             complement(9192..9620)
                     /gene="ruvX"
                     /locus_tag="PLF20_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOX80605.1"
                     /translation="MGRILAIDYGQKRAGLAVTDELQLIPNSLVSLSATEVIPFLKDY
                     MKHEKVDVIVVGQPYTMDYLPSASERYIAPFIKQLIKAFPEVKIERYDERFTSQLAFQ
                     TMIDSGISKKARKDKATIDRISAVIILQSWLDARGIAKNS"
     gene            9960..10775
                     /locus_tag="PLF20_06085"
     CDS             9960..10775
                     /locus_tag="PLF20_06085"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOX80606.1"
                     /translation="MEALKELIEKVWDNRTLLYDNECAQAIRQTIQLLDKGLLRVAEP
                     IDGRWIVNEWIKKAVILFFPLSGYESSVAGPMEFYDKIPLKRGYKDLGVRVVPHAIAR
                     YGAYLARGVVLMPSYVNIGAYVDEGSMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEP
                     VQASPVIIEDGCFIGSRCIIVEGAHIGPESVIGANTVITASTRIIDVTWQEPVIYRGE
                     VPPRSVVIPGFYEKQYHAGKFYLPCALIIGQRKPSTDLKTSLNQALRDFDVPV"
BASE COUNT         3176 a         1922 c         2206 g         3536 t
ORIGIN      
        1 acggtggtgc ttttgtaaaa tattgatatg caattatagg atcaggagtt ctgaatgtat
       61 tggacaggca acctgaaata tcgattttct ttcatgattc atgacatggg aactgtatcg
      121 aaatggcagt aatgaatgca ggttggcaaa gttttacata gaaagggatc gataaataat
      181 agttgattaa ccaattggtt aaattgattt gattaatttt gctaaaaatt gagaaaaagg
      241 gaatgttaag tgtttgcagc tttagtgaac aagctatcat tgatttttgt gaagtaactc
      301 tcgaccacaa tgtagtgcat gatccggcat atatgaaatc acagagtaaa aaagcggttg
      361 tgccaggaat gttgatattg tcgaaagcga ttggtttggc tgaggagatt atatacaaag
      421 gtgcaaatac ttttcaattg tacttgaact ctatggtagg agccaatgaa tttttaacca
      481 ttggttataa tatgattgca gaaactcctg tcaaggccga gattgtagcc cgaaattcga
      541 ccgatgcttt gcgtcataga ggaaatccct ctaccatttt caaaagggat catgatccac
      601 agtttgaata cttggggaat cggcgtcatc ttgaaaccga tgctgctcag gtagaatctt
      661 tttcggagat agtgggtttg aaggggaagc ccttaggggc tttactattt agcattgctt
      721 atgccagtca ttctcttttt cgggctatcg aaaaaccagt cagcagaatg gaaagggaaa
      781 ttcgggagta cttgagcaag gatgctcgaa acgacaaaca atcgcctttc tatcaatcta
      841 tttctataca tttgcccgat aatatctatg ttcctcaggg aggatttcct ctcgattttc
      901 tcattcattt cgaaaggata aaagaaggtc gtacttatag tgctcaggta atatgcacgc
      961 aaaatgacag ggatatttat ttttccaaat atgttttgac tgccattccc gatcgtttga
     1021 tcatgcgcat ggccccaaat ctatgaatgc cctgtaaggt tttacatgat ttttagtttc
     1081 aggcattttt gatcgaaaat ataaccttcg agcaaatctc cgcttttcac aggtccaacc
     1141 ccctcaggag tcccggtaaa aataatatct ccctgcttga gagtgataaa ttttgataca
     1201 taagtaataa tttgagaaaa agaaaagatc atatcccttg aattgccctg ttgtatggtc
     1261 tcaccgtttt tttgtagcga aaacgaaata ttttttaaat cttcgaattc ttcttttcga
     1321 ataaaggcac ccagaggggc cgaaaaatcc catgctttgg caatctccca aggtaatcct
     1381 tcttccttgc atttccgctg aaggtcgcga gcagtaaaat ctatcccaat cccaatgctt
     1441 tccacataac caagggcaaa tctttcgtcg atatgtttgc ccaaacggca aatttttaaa
     1501 acaagctcgc actcataatg aagttctttc gtaaattccg gaaaatagaa aggatgatga
     1561 cgcggcagaa gggaggtttc gggtttcatg aaaaaaatcg gattctcggg aagccgattg
     1621 tgcaattctt ccacatgtgc tttgtaattt cttcctatac agatgatttt catttcgagt
     1681 ttgattattt aatctttaat cgatgataaa gtaaaccaaa acgccaacat tcggatcggt
     1741 atgaaaaatg aattttctat agaattcaat ggtttgcaaa tctaagaata atgatgttta
     1801 ttgtttacaa gatttgactg ttttactaaa actagaggca aatttgataa ttatgattta
     1861 tttttgctta tgcaaactca atggaatgag tggataaaag gattcagggc ttacttgact
     1921 cttgagaaag gtctttcagc taatactttg gaagcttatg ttcatgatgt ccaactttta
     1981 actgaatatt ttgaaataac ttcgatagta aaatcaccag ctgaggtcag cagagaagat
     2041 cttagccgtt ttgtggaata catttatcaa ctggggcttg aagcaagttc tcaggcaaga
     2101 attatttcag gaattcgtgc ttttttcaag tatatgttgc ttgaaggcac tttgaatgag
     2161 aatccggctg aattattaga aatacctcgt ttacaaagga aacttcctgt agttttatca
     2221 gaagaagaaa tcgatcgact aattcgaatt atcgatgtga gtcggcctga agggcaacgc
     2281 aacaaagcca tcattgaaac gctgtatgcg tgcggtttac gtgtttcgga gctgatcgag
     2341 ctcaggattt cgcgtttgta tttggaagaa ggctatatcc gtgtgattgg aaaaggcaac
     2401 aaggaacgtt tcgttccgat tggagaagaa gctattcata acatagtgct ttacatcgag
     2461 aatgtgagga atacattgaa ggttcaaaag cagtggagtg atatagtttt tctgaatcga
     2521 agaggcggca agatgagtcg tgtaatggta tttttaataa tcaaagattt ggcagcaaag
     2581 gcaggtattt cgaaaaaaat aagtccacat acctttcggc attcatttgc tactcatttg
     2641 gtcgagaatg gggcaagcct cagagccgta caagatatgt tggggcatgc atccatcact
     2701 acaactgaga tttatacaca tctcgacaag gagtatttac gtcgaacgct gctggatttt
     2761 catccttttt acgtaaaaaa atagacgctt cccgtaaagg aagcgcctat cctatgtcaa
     2821 aaaaatattt attattttag cgctacaaag atacaaaaaa attgtgaata acaatcaata
     2881 caccggaaaa aattaatttt cggagggggg taagaggtaa gcttttactt tcgcttcggt
     2941 agcaatagca tcgatttttt tactacgagt agccaaacct gacagacagg ctgcaatagt
     3001 attccttaca taattcgttt ttttatcagg aatttcgtat tgtttgatgg caatattcgc
     3061 aatttccttt gcagtcagag gatgggtggc atttttaagg gtatccataa taaaactcgt
     3121 ccaaggaatt tttataccca gctcctcact ttgttttatc gtttttttcg agctttttgt
     3181 ttcaacctta tcggctttct ttcctgcttt ttttagtttt ttcgaggttt gaatttctat
     3241 gttttcctcc acaacctctg taggcatttc ttgagcaagt gattcggttt caaagggctg
     3301 aggtatgttt tttgaaactt cagtctctgg agtttcacct ttttccaaaa caggtttttc
     3361 ttttcttgga cgacctattt tcttaggggc tgcaagggcg gcttcaggcg cagcaatggc
     3421 ctgaccggca gtttgttgat ctttccttgg ccttcccctt ttcttttttt cggtatatac
     3481 ttcacccgct atgggttcat tcgtagcagt aacctgtttt ggcttaattt cggtaatttc
     3541 aacaggcgat tcgaatttac tgagaatctg ttggcaatgc gataatctgc gttgcgcgcg
     3601 ggccagttcc ctcatataga attcttgcag ttcactggct tcctgatcac taagaattaa
     3661 tgttttcatc tggtaaggtg tttaatagtt ttgagtgata atttatttat tttcagagaa
     3721 taagataaat gttttgcaaa gaaaaacttg tatctgaatt caggctacaa aaatataaat
     3781 aatttttaaa aatgcaatct tttgtaaaaa aaaatcatta atttaacttt cttgaaccga
     3841 aagaaaatat aatagatttt taatcaataa attatctaaa aatatgacgc tctaaatttt
     3901 tcataaaaac ccattcctct actaattgcc ctgttctaat tcttgttgtt tcattgaaat
     3961 ttagtttttt gaaaccattg ttttccaata ccttaattga gccaatattt aaactgtaaa
     4021 cttttgctat gatttcgtcg gtatttaaat agaatgctaa ataatgaata aagttcttta
     4081 cggcatcact cataattcct tgattccaaa acgggactcc aagccaataa ccaagttccc
     4141 atgtggattt acccacgaat tgtatgtatt gaacaccaat cgaaccaaca gcttcatgat
     4201 ttatatcgat ggccaaaaca aatgcatctt gtgcattcag gcagaaataa atatactcct
     4261 ctgcgtcttt actggtgtag ggatatggaa aggaaggccg caaaaaagtc gccacccttg
     4321 aatcattggc ataatttgcc agtgatgttg tatcggccca tgaaaaaggc cttaaggtaa
     4381 atttgttgag tttgatgatg attcttggaa tcattcattt gttagcttaa gatgcttttt
     4441 ttcgatttta ttgaaatcgg gcattgatcg aacattccat ttatcgataa ttaccccgtt
     4501 ttttattaaa attaatccag gatttgccct taccatcatt ttgagtgtga tatcgtcgat
     4561 attgaaaatt tcagcctgga tatcatattt tagtgcaaaa tcctctatct ctttgttgag
     4621 agctgaagtt agtattatgc atgtatatcc cgaattttct gcactacgaa tgaaatcaga
     4681 caactttttc aaggatttat caggtgctga atgtaaatca taaattgtgg caataaaaag
     4741 aaaatcggga taattgaggt agtaaaggct aacatcattg cctaaagtat cactaataag
     4801 caatagtgta ttatctgatg aaggatggct tactactctc gaactcacga attcccattg
     4861 ggacatccat atagagtctt tatacggaaa atcattggaa gggaattctc tttcctctcc
     4921 tgtggtttta ttcctatatg ttacataata ttctgaagat tgttgactta ccgttgaaac
     4981 gatatttcct tttttccaag gcaaaaaatc gataagaggt aaatggtaga atgaccagat
     5041 cgaaaatccg gcaaatatgc aggttactgc tatgaataag gctgtacgtc gaaaaaaggg
     5101 tggttttgcg tttcgggatg tataaaaaag tattgctact aaagcaagta aaacgatatt
     5161 tttaaaaaag gtttgccaat tggtgagttt aatggcatcg ccaaaacatc cacaatcgga
     5221 aacagcatta taaagggcat cataaaaggt aatgcaggta aaaaaaacca tgaagagcaa
     5281 aataagccat tgtatgagtt ttgtctgata attgagaatt aatccggcac ctattacaaa
     5341 ttctgccgtg catagaagaa tggaaagtgc gagtgcaaaa ggcaaggccc aatcccagcc
     5401 ataagcaatc aaataatcct ctatcttgaa catagtgccc aatgggtctt ggcctttaac
     5461 aaatcccgaa aagataaaag tcattcctaa aagcagtctg ctgacttgat agagtttttt
     5521 catcataagt caaaagaaat ttttattctt acttgttttc tgatatcttg atcaatgaga
     5581 aaatagcgta attcataata tccaaataat tcgattcaat tccttccgat accagtgtaa
     5641 gtccgctatt atcttcgatt tgcttgatgc gttttatttt catcagaatg atgtcggtta
     5701 atgagctcag gcgcatttgc cgccaggctt catcgtaatc gtgattttta tccagcatga
     5761 gttgtttggc tttttgcgac cacctgttgt atgaattgag ataatctata ttgctggggc
     5821 tggcattctc tggctctggg ccaagttcgt gctgaatcaa ggcaatgagt gaataattgt
     5881 agatgcctat aaattccggc ttaatccctt cgttgatacg ttgttcgctt gatgattgta
     5941 aagtacgtat tcttcttgcc ttgatataga tttgatcggt aatggaagga agccgcaaaa
     6001 ccctccatga aggcccataa tctttcaatt ttgcctcgaa taccttgcgg cattctgcca
     6061 ccgcttcatc atattgcaat aaggtatttt gcatgatttt cttaatttcg cactataaat
     6121 cgaatactga tgaatttggg cagtaaagat acaaattccc ttacaactcg gggcttactc
     6181 attggcgaga aaggtataat gattcctttt cccgccgtta tgggcatatt aaacattact
     6241 caggattcat tctatgatgg tggcagatat ctaactgaag atcaatggat aaatagaact
     6301 gcactcatgc ttcaagaagg tgcttccatt atcgatgtag gtgccgcttc tacacgccct
     6361 ggagctcagg aaatacccga aatttttgaa atagaaaaaa ttagaaaagt cgttcatacg
     6421 ctcatacaac atttccctga tattatcatt tcggttgata cttaccgctc gctggttgca
     6481 caaaccgccc tggatgaagg tgcttccatt attaacgata tttcggcagg cgacttcgat
     6541 gccagcatga ttccacttgt agccagccgg aaagtccctt acatcatgat gcatattctt
     6601 ggcactcctg ccaccatgca aataaatcct cattacaatg atgtagttac ggatattatc
     6661 gactatttta agcatagaat cgaagtttgc aaccgatatg gattggatag tctaattctc
     6721 gatcccggct tcggtttcgg caaaaccttg aagcataatt atacactatt gtctcatctt
     6781 tcctcattta ttaaactggg ttatccggtt ttagtgggcg tttcgcgtaa agcaatgatt
     6841 tataaattat taaatgttca acccgaagaa gccctttttg ggactgttgt tgccaatgcg
     6901 cttgctttat tgaatggagc tcatattata cgtgttcacg acgtaaaacc gaccattcag
     6961 gccataaaaa ttctcgatga atacaaggat tccctgacta atccttaata atttatttga
     7021 agacagtttt aactggtttt aatgaatgct tgaatattct ttacatgttt ttagtaaaca
     7081 aaataaccga aattcgtata gcaaataccg aattaatttt caaatagagt aaaaaatgaa
     7141 attttgatcc atattaacga ttaatgaata catcacaagg aaatgttaat caaaaccata
     7201 ctcccattat ttattacttt tcgctggata gatgccatcg atattttttt ggttgccctg
     7261 ctactgtacg aattttataa tttggtgaaa ggtacgggag ccatcaatat ttttatagga
     7321 atagtcgctg tgattatttt atggaagatt gtatcggcac tcgagatggt gcttttgagc
     7381 gagatattag gtgccttcat cagtgtagga tttattgccc taattgtggt atttcagccc
     7441 gaaatcaggc aatttctttt gttattgggt aatccttcgt ttatacagga aaggagacga
     7501 aaattctttt tttggaggat tccgagagcc aatgatgaac gtcttgctat agataatgtt
     7561 gtttcagcct gccagaggat gggaaattcg gctactggag ctctcatagt gatagccaaa
     7621 accaacgaat tgaaagaata tgttcagtcg ggtgaaatca tcgattcctt gatttctgtg
     7681 cctttactgg aaaccatttt ttttaagaac acacctttgc atgatggagc agcaattatt
     7741 gtaaacaatc gcatcaagtc ggcacgctgc attttacctg tatcgagtaa caataagatt
     7801 cccattgatt tagggttgcg tcatcgagct gctataggaa ttaccgaacg aaccgatgct
     7861 atagctctga ttgtttcgga agaaactggc gagatttcgg tagccaagga aggcacactt
     7921 gtacaaaaca ttagaccagc tcaggtgaaa gatttcctcg aaaaggaatt tgcccctcac
     7981 gaagctgcac ctcgaaagag aaaggtttcg aaaaaggaat gaaaccctat gatttattat
     8041 tgctggtttt tctccaaatt attcttatca gcatcagctt gtaatatttc aagtatctcg
     8101 gcattactca tccccaaaaa ctgtatcgat ttttcggcat ctcttctcag gtcgtgattg
     8161 gggaattttt ggatgaataa catataattt tcacgggctt tgtcataacg ataaagatag
     8221 ttgtcgaaga cgaaggcttt cataaacatg gcttcggcag ctttaggaat ctcgggaaat
     8281 gaaatgatca ggctgtcgta aaacttgata gcctcttcga agttgccgat ggcggtacac
     8341 aactgagccg ctctgaacat atactcggca gaaaggctgt cgtgtggata attgtgtata
     8401 tattcagagt ataactcgat ggttttttgt gctttttgtg gcattattat gccagtagtg
     8461 tcattaaaga gctgtttttc gatgttggtg atttcgtttt ttaaatgctc ctgtttgttt
     8521 gaacagcccc atataactac aacaacgatc agggctaatg acaatacgta aatattttta
     8581 ttattcatgg ctttgtgaat taatggttat ctcttctgtt tttttgatct cgggaaaatc
     8641 atcctgtatt ttgtctctat gttacctaat gcaatattgt tgagctttct tgcaagcatg
     8701 gtttttcgca taggacttag gtaatcggta aagagaattc cttctaaatg atcatattca
     8761 tgctgaatgg tacgtgctac agcaccttcg aaccattctt cgtgttcgtc gaagttttcg
     8821 tcgaggtagt gcaaaaggat gcgttttgga cggcttacat cttcatgtat gtcaggaatg
     8881 ctcaagcaac cctcgttgaa tagccaggcg tctcctcctt catttatttt ataggcattt
     8941 atgaaagttt tcttaaatcc agtggtttcg ggatagtttt tagccagaac agtggcatcg
     9001 atcacaaata tgcgaattgc acgattcact tgaggtgcag ccaaaccaat tccatcagtg
     9061 gcatacatgg tttcccacaa atttttaata aattcttgca aatcgggaaa atcggattta
     9121 atatctgcag ctttttgtct taaaattgga tggccatatg ctacaatggg ataaatcata
     9181 gtttcgttgt tttaactgtt tttggctatt cctcttgcat cgagccatga ttgaagaata
     9241 atgacggcac ttatgcgatc gatggtggct ttgtcttttc tggctttctt tgaaatgcca
     9301 gagtcgatca tggtttgaaa tgcaagctga gaggtaaatc tttcgtcgta gcgttcgatc
     9361 ttgacctcgg ggaaagcctt tatcaattgt ttgatgaaag gagcaatata acgttccgat
     9421 gctgaaggaa gatagtccat cgtataaggt tgtcctacca ctattacgtc cactttttca
     9481 tgcttcatat aatctttcag aaaaggaatg acttcagtag ccgaaagtga gaccagggaa
     9541 ttcggtatga gctgaagctc atcggtgact gctaggccgg ctcttttctg tccgtaatct
     9601 atggctaata tccttcccat aattttctgc aaaattagtt tttttgcggt taggataagc
     9661 gaatagggat ttcctcctag aaaatatggt gttttcgcag tcagaaacgt tgctatcagg
     9721 atttttcatc gaaatatgta aaagaaaata aatttcagac aaaatgcaaa ggcttattga
     9781 attttgcaat taaatttaca tgattcaaga aataattcat tgaaataaaa cattttatat
     9841 tgatagattc ggttattctt caataaggtg tttagtctgg ctgtggatga gatatatgaa
     9901 tcgattggcc ttgccatgaa tagcttttgt aactttgtat ttcaaaaacg agtcgatgaa
     9961 tggaagcctt aaaagaattg atcgagaaag tatgggataa tcgaacatta ttgtacgata
    10021 atgagtgtgc acaggctata cgccagacca ttcagttgct tgacaagggc cttctaaggg
    10081 ttgctgagcc aattgatgga aggtggatag tgaatgaatg gataaagaaa gcagtgattt
    10141 tgttttttcc tttgtcaggc tatgaaagtt cggttgctgg cccgatggaa ttctacgata
    10201 aaattcctct aaaacgagga tacaaagatt tgggggtaag ggtcgttccg catgccattg
    10261 ctcgctacgg agcctatctt gcccgcggtg ttgttttgat gccatcttat gtaaatattg
    10321 gtgcttatgt cgatgaaggg agcatggtag atacctgggc tacagtaggg tcgtgtgcgc
    10381 aaattgggaa gaatgttcat ttgagtggtg gagtcgggat agggggggta ctggagccag
    10441 ttcaggcatc tccggtaatt atagaagatg gatgttttat aggttcgcgc tgcatcattg
    10501 tcgaaggagc tcatattggt cctgaatcgg tgataggcgc caatacggtg attacggcaa
    10561 gtacccggat tatcgatgta acttggcaag aaccagttat ttatagggga gaagttcctc
    10621 cacgttcggt ggtcatccca ggtttctatg aaaaacaata tcatgcaggt aagttttatt
    10681 tgccttgtgc actgataatt ggtcagcgaa aaccttcaac tgatctgaaa acttcgctca
    10741 atcaggcatt acgtgatttc gatgttccgg tctgagtttt gtatattttc gaatttgttt
    10801 ctttcggttt ttttatattc ctttatattc cgtcagattt
//
LOCUS       DAOORU010000076        10791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7627_length_10791_cov_5.101434, whole genome shotgun sequence.
ACCESSION   DAOORU010000076 DAOORU010000000
VERSION     DAOORU010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10791
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7627_length_10791_cov_5.101434"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="PLF20_06090"
     CDS             complement(<1..1845)
                     /locus_tag="PLF20_06090"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HOX80607.1"
                     /translation="MKIHKIFSLLIFGLSYFPLISFGQARLQFIHNSPDVSLDSIDIY
                     VNKTLMLDNVKFREASEFIQGPSGIPLLIEICPGNSINSESAFYSTAVMFSQGKTYIA
                     IAQGIIDGNGYHPWQPFELQIIDYGRETSNTPGQTDVLFFGGSTDAPAFDIIETTSLG
                     LLSDDLTYGQFDGYKELTPANYVLEIKDSTSGTRYFRLQFDLTTYSGQAFVLMASGFL
                     VPGLNNNGENLGFYIVSANGGMGVSLPQIPDPMASIQIVHLCADRALDSMDVWIGNKK
                     FGADLPFGNSLPFIEILAEKNQVIAIQPAGSTSPDNPLVSLSYTFHDRQKYILMLTGI
                     TSETGYTPAPALQIYPFDSARIEANSPNATDMLVFHGSTDAPTIELYETGIGLGLLME
                     NLEYGNFASEGYLSLTTADYVIEVRNKPGNEVLYTYSLPLSALGLQGEALSLCVAGFV
                     NPTNNSNGPAFGLYLSGKNGGALFELPIYIPPTTSFVQFIHNIADTSAQSLDIWMNNQ
                     MLLDNHLFQKASPYLEVPANQNLTFSVLPQTSISPDNPLTSLNLTLESGKHYIILLDG
                     IASSSGYSPLTPVSMEIFANARTIALNNTKIDVLFHHGSIDAPEYSVFD"
     gene            complement(2094..2600)
                     /locus_tag="PLF20_06095"
     CDS             complement(2094..2600)
                     /locus_tag="PLF20_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX80608.1"
                     /translation="MSEIEFNYQLLSLQNSLNSFALSLTKNVDEASDLVQDTYLKAII
                     YRDKFDAGTNLKAWTYTIMKNTFINNYRREVRLNAFIDSTDDIYHLNAKSTMEAERPD
                     SSLHAKEIQKSIQSLGEEHRIPFLMHTRGYKYKEIADQMNLSIGTVKSRIFFGRKKLM
                     EILRDYQN"
     gene            complement(2841..3743)
                     /locus_tag="PLF20_06100"
     CDS             complement(2841..3743)
                     /locus_tag="PLF20_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOX80609.1"
                     /translation="MSKYLIKDIERFTGIKAHTLRVWEKRYHVLKPCRTSTNIRTYDD
                     DDLKRILNISILNRHGYRISEIIKLSEEEICEKVMQLGQRCADTHSQIEKLIMAMVQM
                     DEEIFQEVFDQSVKKSGFEDTFYYLIFPFLERTGLLWQSGSILPAHEHFISNLIRQKL
                     IVAIDSILSPRKPNALHSLLFLPEGEWHEIGLLFTYYLIKKWGHYTLYLGPHLPIENI
                     ESTLLKHSFDFLVTQFINGISGNDLNLQIQKIVKICKGKKVLLSGGRLIETPFDLPPD
                     FFIYENTQQLKEIIDNQYILKIKS"
     gene            3939..4451
                     /locus_tag="PLF20_06105"
     CDS             3939..4451
                     /locus_tag="PLF20_06105"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOX80610.1"
                     /translation="MIISSEGIILKTTCSEDVELKRLLENYHDIRYMSEKRIYPSSMP
                     SEYIFRIEAGANLIGEVSLTNIKWLNRKGELSLALSPAYYRKGYGSITLKLVIRFAFE
                     TLNFHRLEAEIIEGNEASLALFLKAGFVEEGRLREAKFLNGKYHDILRFGLLRKEYDP
                     EWQAGSDSIL"
     gene            4558..5055
                     /locus_tag="PLF20_06110"
     CDS             4558..5055
                     /locus_tag="PLF20_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80611.1"
                     /translation="MKNVIGLLLIGGMLLAMSCNSDRKAGPRRITLQSDSMMMIADSM
                     PFYIGKNLYLVIMDYPAPKPALTLGQEIGFLEERAIPGYQHIDSLRRRGIPVFGGVMA
                     LKQGCAFIIQANDNLELQKFLKDNPLTETAQVTVIPLVSFGESLFRHQEKLQKLKESQ
                     TKQRE"
     gene            complement(5065..6237)
                     /locus_tag="PLF20_06115"
     CDS             complement(5065..6237)
                     /locus_tag="PLF20_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HOX80612.1"
                     /translation="MTEPGRYQQRGVSSNKEDVHAAIAAIDKGIEPKAFCKIIPDVLG
                     GNPEYCNIMHADGAGTKSSLAYIYWKETGDLTVWKGVAQDAIVMNTDDLLCVGLFDNL
                     LISSTIGRNAHFIPGEVIHAIIQGTEEVLQWLRRMGITIYSTGGETADVGDLVRTIIV
                     DSTIIARARRSDIITNANIRPGDVIVGLASFGQANYESSYNSGIGSNGLTSARHDVLH
                     KIYRRKYPESFDPNLPESLAYCGKRFLLEASPVEGINIGKLLLSPTRTYAPVVKEIIA
                     NCKPAIHGMIHCTGGGQAKILHFIENCRIVKDNLFEIPLVFRLIQEESGTMWSEMYKV
                     FNMGHRLEIYVPFEIAAEIISLAQKYDIQAQVVGRVEKSSENSLIISTAQGVFEYK"
     gene            complement(6242..8719)
                     /locus_tag="PLF20_06120"
     CDS             complement(6242..8719)
                     /locus_tag="PLF20_06120"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOX80613.1"
                     /translation="MKKITLLILLSIFVLGLILPVRAQKNKQVEAADQAFANMMYTTA
                     LKNYRKAYSKIKDKDERNRILYQTAECYRIMNNYRMAEPTYRRLLTQKYEEKQPLLLL
                     HLADILRFNRKYEQAIPFYQSFSELQPDDPRGPLGLQSCQLNETLSKTPTRHRITNEK
                     ELNSRYDEFSATYANTGYNEILFTSNRNGSTGKGKDEWTQKYFSDLYYVKKDAKGKWT
                     KVTKADEEGIINTAGNEGTPFFNSRFNVMYFTRCATEPKKTNTCRIYSSKRTGKNFSE
                     PVPVDIAIDSIYSIGQPTLSTDELMIIFSSDMPNGKGGRDLWMATRQRKTDGFGRPKN
                     LGDSINTLGDELFPFLRYDTVLYFSSTGHPGLGGLDIFKAYFRTDKEGRKYFTRPIHM
                     GVPLNSNFDDFGIVFHPEAEEEGFFASNRQGGRGSDDIYSFVVEPVVFTLEGLVKDES
                     TLQPIAGALVEMKVSDGSQHKSNTSERGRYFFNQNQVKPNRNFDLSVSKEGYFTKTGS
                     ISTIGLESSKDLVLDFVLQPIPRKPIVLPEILYDLGKWDLKPQYQDSLQGLITILEKN
                     PNLEIELAAHTDARDTEEHNDILSQKRAQSVVDYLVQRGIDPDRLTAKGYGERVPRVL
                     TRDIKKAGYTFKAGTRLTESFIDSLPNREVKEAAHQLNRRTEFSITGTNFKSKPKPQG
                     QQFAPKIEIVTTPPKNSVTYILSAKKNRMLIPASIDTYSLEFLYEPNTKYVSISMDAA
                     LDLLKQNIITKDDFQGNPEEIISEGQIKRGSVLTLPSLRIGRKMIYDVDVIVEPKQDE
                     KLILNNEILSRFGAFTINEDTREIIFE"
     gene            complement(8747..9055)
                     /locus_tag="PLF20_06125"
     CDS             complement(8747..9055)
                     /locus_tag="PLF20_06125"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HOX80614.1"
                     /translation="MQVPFLIFDISSGELILILLVALILFGPKQIPAIARSIGKGINE
                     LRKASDQIKNEVMQESEKINSQITSDVIIENNRGNKELINKTEEESIKPPENPLPPAG
                     "
     gene            complement(9215..>10791)
                     /gene="secF"
                     /locus_tag="PLF20_06130"
     CDS             complement(9215..>10791)
                     /gene="secF"
                     /locus_tag="PLF20_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOX80615.1"
                     /translation="DLANILQSGRLPAPARIVQEEVVGPSLGKEAINAGMKSFAAAFF
                     IVLVYMFLYYNRGGLIADLALCLNLFFIFGVLASFGAVLTLPGIAGIVLTMGMAVDAN
                     VIIYERILEEVRAGKGLRVAIADGYKNSYSAIIDGNVTTLLTGIILFIFGSGPIQGFA
                     TTLIIGIISSLFTAILVSRVIMEWWADKGYKLQFDNKFTRGFLTKANFDFIGIRKYAY
                     IFSIALFLLAIGSFIFKGLNYGVDFTGGRTYVVRFDRDVNVPEIRELLANEFGEAPEV
                     KTYGPSNQVKITTKYLIDEEGREVDSLIDRKIYNALRGLYANEINFSHFASDLNQSGK
                     LIGILTSQKVGPTIAYDIKRNAIMAVIFALLGIFIYIAARFNKWQFGLGGVISLFHDT
                     IIIIGLFSLFHGILPFNLEVDQSFIAAILTIIGYSINDTVVIFDRIREYNKLYPKRDL
                     KINMNAAINSTLARTMNTSCTTLVTLLMMFIFGGETLRGFLFALILGIVFGTYSSVFN
                     ASAIAYDTMKNKWKKK"
BASE COUNT         3109 a         2101 c         2306 g         3275 t
ORIGIN      
        1 atcgaatacc gaatattctg gggcatcgat actcccatga tgaaacagca catcgatctt
       61 tgtattgttc aaggcaattg tacgagcatt agcgaagatt tccatagaaa caggtgttaa
      121 gggactatat ccgcttgaac tagctatacc atcaagaagg attatataat gttttcccga
      181 ttcaagtgtc agattcaggc ttgttagagg attatccggt gaaatggaag tttggggtaa
      241 aacgctaaat gtcagatttt ggtttgccgg aacttcgaga tagggagaag ctttctgaaa
      301 cagatggtta tcgaggagca tctgattatt catccagata tcgaggcttt gagccgaggt
      361 atcggcaata ttatgtatga actgtacaaa tgaggtagtg ggaggaatat aaattggcag
      421 ttcgaacaag gctcctccat tctttccgga gagatacaaa ccaaaagcag gaccattgct
      481 attatttgtg ggatttacaa aaccagcgac acaaagcgac aaggcttcac cttgtaaacc
      541 gagtgctgaa agtggcaaag aataagtata taagacttca ttacccggtt tatttctaac
      601 ttcgatgaca tagtcggcag tggtaagaga taaatagccc tcagatgcaa aattcccata
      661 ttctaaattc tccattaata aacccagacc tattcctgtt tcgtaaagtt cgatagttgg
      721 agcgtcggtc gaaccatgaa ataccaacat atcggtagca tttggcgagt tagcttcaat
      781 tcgggcacta tcgaaaggat aaatctgtag ggcaggggcc ggtgtatatc ctgtttcact
      841 tgtaatcccg gtaagcataa gtatatactt ttgtcgatcg tgaaaagtat aggagaggga
      901 aacgagagga ttatcggggg aagtgctccc agcgggttga atcgcaatca cttgattctt
      961 ttctgcaaga atctcgataa aaggcaaaga atttccgaaa ggtaaatctg caccgaattt
     1021 tttatttcca atccaaacat ccatgctatc gagcgctcga tcggcacaaa gatgaacgat
     1081 ctggattgat gccattgggt cgggtatttg aggcaaggac acacccatac ctccattcgc
     1141 cgaaacgata tagaaaccca gattttctcc attgttgttt aagccgggga ccagaaatcc
     1201 cgaagccatt agaacgaagg cttgacccga ataggttgtc aaatcgaact gtaaacgaaa
     1261 atatcgtgtt ccactggttg aatcctttat ttcgagcaca tagttggctg gggttaactc
     1321 cttgtaacca tcgaattgcc cataagttag atcgtcactc agaagtccta aagaagttgt
     1381 ttctataatg tcgaaggcag gagcgtcggt tgagccaccg aaaaacaata catcggtttg
     1441 gccaggtgta ttcgaagttt cacgcccata atcgatgatc tgtaactcga acggttgcca
     1501 cggatgatag ccattcccat cgataatgcc ttgcgcaatg gctatataag ttttcccttg
     1561 agagaacatg acagcagtgc tataaaatgc actttcggaa ttaatcgaat ttcccggaca
     1621 aatttcgatt aaaagcggaa ttcccgacgg cccctgaata aactccgaag cctccctgaa
     1681 tttcacatta tcgagcatca gagttttatt tacataaata tcaatcgaat ctaaactcac
     1741 atcgggtgaa ttatgaataa actgtaaccg ggcttggcca aaagaaatta aaggaaagta
     1801 acttaaaccg aaaattaata aagaaaaaat tttgtggatt ttcattgatt attaagtttt
     1861 taatggctat ttttcaaaat atcaggcatt tgaatccccc cttcaaaccg cattctgtag
     1921 tcatcgcaat aagttaggcc acgaaattac atttttttcc ataactaaag aatcgaatac
     1981 caatcaaaat cttttaaccc ttatttgtta aaagtgtttg gaattgataa aaaatgacct
     2041 ccatagaggt catttcatat atgcgccata tatattctaa ctcgttggaa aatttaattt
     2101 tgataatcgc gtagaatttc catcagtttc tttctgccga aaaaaattct actctttaca
     2161 gtacctatcg aaaggttcat ttgatcggca atttccttat acttatatcc acgcgtatgc
     2221 atcaagaatg ggatgcgatg ttcttcgccg aggctttgaa tacttttctg tatctccttt
     2281 gcgtgtaggc tcgaatctgg cctttcggct tccatggtgc tctttgcatt caaatgataa
     2341 atatcatcag tgctatcgat aaaggcattc aagcgaacct cccgacggta attgttaatg
     2401 aaggtgtttt tcatgatggt gtaagtccaa gctttgagat ttgtccctgc atcaaacttg
     2461 tcgcggtaga ttatcgcctt caaataggta tcctgtacca gatcgcttgc ctcatcaaca
     2521 ttcttagtca atgataaagc aaaactattc aggctgtttt gtaaacttaa caattgataa
     2581 ttaaactcta tttcgctcat aatgtggaat ttttactaat tagaaaattt ctaaagtgtt
     2641 taaattttat ggcacaaatt taaacaatat ttctaaaata tgttaacact ttcctatata
     2701 aatttttaaa tatttattca aaaaatgcca ttacattgat ttacaatata aattggttgt
     2761 ataatttaac atttattaac ttttcttgct taaaaaagaa aagccaatgt aatttatcaa
     2821 tggagaagga agaggaagga ttagctcttt attttcagta tatactgatt atcaatgatt
     2881 tccttcaatt gttgggtgtt ttcgtaaata aaaaaatcgg gaggaaggtc gaaaggcgtt
     2941 tcgataagtc gaccgccgct tagaagaact tttttgccct tgcatatttt gactattttc
     3001 tgaatctgaa ggtttaaatc gttgccagaa attccattga taaattgggt gaccaagaag
     3061 tcgaaagagt gtttgagtaa ggttgattcg atattttcga tgggaagatg aggcccgaga
     3121 taaagtgtat aatgtcccca tttttttatc agataataag taaacaacaa tcctatttca
     3181 tgccattccc cttcgggaag aaaaagcaat gaatgcaagg cattaggttt tcgcggggag
     3241 aggatagaat cgatggctac gattaatttt tgcctaatca ggttactgat gaaatgttca
     3301 tgagcaggca gtatgcttcc cgattgccag aggaggccgg ttcgctctaa aaaggggaat
     3361 atgaggtagt aaaaggtatc ttcgaaacca ctttttttaa ccgattgatc gaaaacctct
     3421 tgaaaaattt cctcatccat ttggaccatt gccatgatga gtttttcgat ctggctatgc
     3481 gtatcggcac atcgctgacc gagctgcatt accttttcgc aaatttcttc ttcagaaagt
     3541 ttgatgattt cagatattct atagccatgc cggttaagga tagagatatt gagaatacgt
     3601 tttaaatcgt cgtcgtcgta agtacgaata ttggtcgaag tacggcaggg tttcaggacg
     3661 tgataacgct tttcccagac tctgagggtg tgagctttaa ttcccgtaaa tctttcaata
     3721 tctttaatca aatattttga catggttaaa cttttgaaat aagaaagctg tcgagcaaca
     3781 gtctatcgtt attttaacaa ttggaatgat tatatgttta ataagaaatt tcgaagggtt
     3841 gaaaatgttc gatgaaataa catattggcc tgtttattcg attttggcga gaaaatacaa
     3901 tttgtttatc tttgtcattc aaataaataa atataatcat gattatttcg agtgaaggga
     3961 ttattttgaa aaccacctgt tccgaggatg tggaattgaa gcgtctttta gagaattacc
     4021 acgatatacg gtacatgtcg gaaaagcgaa tttatccatc ttccatgcca tcggaataca
     4081 tcttcagaat tgaagccggg gctaatttga ttggcgaagt tagcttgacc aacatcaaat
     4141 ggttgaatcg taaaggcgaa ttaagtttgg ctctttcacc cgcatattac agaaagggtt
     4201 atggaagcat aactttgaaa ttagtcattc gttttgcttt cgaaacttta aattttcacc
     4261 gtttggaagc ggaaatcatc gaaggtaatg aggcttcact ggcacttttt ctgaaagcag
     4321 gttttgttga ggaggggagg cttcgtgaag caaaatttct gaatgggaaa tatcatgaca
     4381 ttcttcgttt cgggctattg cgaaaggaat atgatcccga atggcaagcc ggttcggatt
     4441 ctatcctcta acattcttat ttttccataa acttcttggt cgtttgaaga atatgtttga
     4501 attcatttac ctttgctctt aaagatcata caatcgagtt cattataaag actcatcatg
     4561 aaaaatgtta taggattatt gcttattggt ggaatgttgc tggctatgtc gtgcaacagt
     4621 gacaggaaag ctggtccacg tcgaattacc cttcaatcgg attcgatgat gatgattgcc
     4681 gattcaatgc ctttttatat tgggaagaat ctttatctgg tcatcatgga ttatcctgct
     4741 cccaagcctg ctttgacact gggtcaggaa atcgggtttc tcgaagaaag ggcgattccg
     4801 ggttatcaac atattgacag cctcagacgt agaggaatcc ctgtatttgg aggagtaatg
     4861 gctttaaaac agggttgtgc cttcattatt caagccaatg ataatctgga actccagaaa
     4921 tttttgaaag acaatccgct tactgagact gcccaggtaa cggtaattcc tttggtatcg
     4981 tttggcgaaa gcctgttccg gcatcaggaa aaactgcaaa aactaaagga aagccaaaca
     5041 aagcaaaggg aataaccgag gtattcattt gtattcgaat actccctgag ccgtagagat
     5101 gatgagggaa ttttcactgg atttttctac tcttccaacc acctgagcct gaatatcata
     5161 cttttgagct aggctaatga tttcggcggc aatttcgaag ggcacataaa tctcaaggcg
     5221 atgccccata ttgaatacct tgtacatttc tgaccacata gtacccgatt cttcctgaat
     5281 caaacgaaaa acgagtggaa tctcgaaaag attgtctttt actattcggc aattctcgat
     5341 aaagtggagt attttagcct gacctccgcc tgtgcagtgt atcattccat gaatagctgg
     5401 cttgcagttt gcaatgattt cttttaccac tggcgcataa gtacgggtag gggaaaggag
     5461 cagttttcca atattgattc cttccacagg gctagcttcg agtagaaacc tttttccgca
     5521 ataagccagg ctttcgggaa ggttaggatc gaagctttcg ggatattttc gtctgtaaat
     5581 cttatgcaga acatcatgcc tggccgaggt cagtccattg ctgccaatgc cactgttgta
     5641 tgaagattcg taattagcct gtccaaagga agccaaacct actatgacat cgcccgggcg
     5701 gatatttgca ttggtgatga tgtctgatcg tcttgcacgg gcgataattg tcgaatcaac
     5761 gataattgtg cgtaccagat cacccacatc ggcagtttct cctccggtcg aatagatcgt
     5821 aattcccatc ctcctcagcc attgcaacac ctcttctgtt ccttgtatga tcgcatgaat
     5881 tacttcaccg ggaataaaat gcgcattgcg gccaatcgtg gacgaaatga ggagattatc
     5941 gaataatcct acacacaaca aatcatccgt attcatcaca atagcatctt gagccacacc
     6001 tttccaaact gtcagatcgc cagtttcttt ccagtaaatg taggctaggc tgcttttagt
     6061 tccggctccg tcggcatgca tgatgttgca atattcgggg ttgcctccca aaacatcagg
     6121 aattatcttg caaaaggctt taggctctat gcctttgtct atggcagcta tggctgcatg
     6181 cacatcttct ttgttcgaag atacgcctct ttgttgatac cggccgggtt ctgtcataaa
     6241 tttattcgaa aataatttct cgggtatctt cattgatggt gaaagctcca aaacgagata
     6301 atatttcatt attcagaatg agtttttcat cttgttttgg ttcgacaatc acatcaacat
     6361 cataaatcat ctttctgccg attcgcaggg agggtagggt cagcactgag ccacgcttga
     6421 tctgtccttc cgaaataatt tcttcgggat tgccctgaaa atcgtctttg gtgatgatgt
     6481 tttgtttcaa aagatctaag gctgcatcca tcgaaatgct tacgtatttt gtattgggtt
     6541 cgtataggaa ttcgagcgaa taggtatcga ttgaagcggg aataagcata cggtttttct
     6601 tggccgagag gatgtaagtt acgctatttt ttggtggggt ggttacgatt tcgattttag
     6661 gtgcaaactg ttgaccttga ggttttggtt tagatttaaa attcgttccg gtgatagaaa
     6721 attcggttct tctgttgagc tggtgggcag cttccttcac ttcacggttg ggtaagctgt
     6781 cgataaacga ttcggtaagt cttgttcctg ccttgaaagt gtatcctgct tttttaatgt
     6841 cccttgtaag cacgcgagga acacgttctc cgtatccctt tgccgttagt ctatcgggat
     6901 cgatccctcg ttgaaccaga taatcgacta ccgactgggc tcgtttttgc gacaagatat
     6961 cgttatgttc ttcggtgtct cgggcatcgg tatgggctgc cagttcaatt tcgagattgg
     7021 gatttttttc gagaatagtg atgagccctt gaagagaatc ctgatattga ggtttcaagt
     7081 cccatttacc caaatcgtag aggatttcgg gaagaacgat cggttttcga ggtataggct
     7141 gaagaacgaa gtcgagtact aggtctttac tgctttcgag acctatggta gaaatgctgc
     7201 ctgttttggt aaaatatcct tctttgctta cgcttagatc gaaattacgg ttgggtttca
     7261 cctggttctg attgaagaaa tagcggccac gttcgctggt attactcttg tgctggctgc
     7321 cgtcggatac tttcatttcg accagtgctc cggctatggg ttgaagtgta ctttcatctt
     7381 ttacgaggcc ttcgagggta aaaacaacag gctcgacaac gaaactgtag atatcatcac
     7441 tacccctacc accctgtcga ttcgatgcaa agaacccttc ttcttcggct tcggggtgaa
     7501 acacgatacc gaagtcgtcg aagtttgaat tcagaggaac gcccatgtgg ataggcctgg
     7561 tgaaatattt gcggccttct ttatcggtgc ggaagtaagc cttgaaaata tcgagccctc
     7621 ctaagccggg atggccagta gaggagaagt aaagtacagt atcataacgt aagaaaggga
     7681 ataattcgtc acccagagta tttatggaat ctccaagatt ttttggccgg ccaaaaccat
     7741 cggtcttacg ctggcgagtt gccatccata aatcacggcc gcctttgcca ttaggcatgt
     7801 cggaggaaaa tataatcatt agctcatctg ttgaaagagt gggttggcca atagaataaa
     7861 tgctatcaat ggcaatatcc actggtacag gttctgagaa atttttcccg gttcgtttcg
     7921 atgaatagat tcggcaggta ttcgtctttt taggctcggt agcacagcgg gtaaaataca
     7981 tcacgttgaa ccggctgttg aaaaatggcg taccttcatt gccagcagtg ttgattatcc
     8041 cttcttcatc ggctttagta acttttgtcc atttgccttt tgcatccttt tttacataat
     8101 acagatcgga gaagtatttt tgagtccatt catcttttcc ctttccggtt gatccattac
     8161 ggttggaggt aaaaagaatc tcattatatc ccgtatttgc ataagtagcc gaaaattcat
     8221 catagcgtga attgagttcc ttttcgtttg ttattcgatg ccgggtagga gttttgctta
     8281 gagtttcatt aagctgacag gattgcaagc caagagggcc gcgtggatcg tcgggttgca
     8341 gttcagaaaa actttgatag aacgggatag cttgctcgta ttttctattg aaacgcagaa
     8401 tatcggctaa atgaagcaat agtaatggtt gtttttcttc gtatttctgt gtgagcagcc
     8461 tgcgataagt aggttcggcc atacgataat tgttcatgat gcgataacac tcagccgttt
     8521 gatataagat ccggttgcgt tcatccttgt cttttatttt cgaataagcc ttacggtaat
     8581 ttttcagggc agtggtgtac atcatattgg caaaggcctg atcggcagcc tccacttgct
     8641 tgttcttctg tgccctaaca ggcagaatca atcccaaaac aaatattgac aacaaaatca
     8701 aaagggttat tttcttcata gctctgaaca attctttttt aaataatcat cccgctggag
     8761 gtaatggatt ttccggcggt tttattgatt cttcttcggt tttgtttatt aactctttat
     8821 ttccgcgatt attttcgata atgacgtcgg aagtaatttg cgaatttatt ttttctgatt
     8881 cctgcatcac ctcatttttt atttggtcgg aggctttacg caactcgttg attccttttc
     8941 cgatcgaacg ggcaatagcg gggatttgct taggcccgaa caatataagg gcaaccagta
     9001 aaataagaat aagctctccc gaactaatat cgaaaatcag aaaagggact tgcataagta
     9061 ttttcattaa agaatcgaaa ataaattaca aagctacaac atacctcaaa taaatggaag
     9121 atttatattt gattcgtcga gcattctggt cgataaaaag caaaataccc gatgacatca
     9181 gccagcgggt attttccaag atatgaatta atagttattt tttcttccat ttgtttttca
     9241 tggtatcgta agcaatggca ctggcattga acactgaaga ataggttccg aaaacgattc
     9301 caagaattag ggcaaacagg aaacctctca gtgtttcacc tccaaagata aacatcataa
     9361 gcagagttac caatgtagta cacgaagtat tcatggttcg ggcaagagta gaattgatgg
     9421 cggcattcat gtttatcttc aaatcgcgtt ttggatagag cttgttatac tctcgtattc
     9481 gatcgaagat gaccactgta tcattaatgg aatatccgat tatagtaaga atggccgcta
     9541 tgaacgattg gtctacttcc agattgaaag gcaagatacc atggaagagc gagaatagtc
     9601 cgatgatgat gatggtatcg tgaaaaagac tgattacacc tcccagaccg aattgccatt
     9661 tattgaagcg ggcagcgata tagatgaata tgcccagtaa ggcaaaaatt accgccatga
     9721 tggcatttcg tttgatatcg taagctatag ttggccccac tttttgtgaa gtaagtattc
     9781 caatgagttt accgctctga ttaaggtcgg aggcaaaatg agaaaagttg atctcattgg
     9841 catacaaacc cctaagagca ttataaattt tacgatcgat caatgaatcc acctcgcgtc
     9901 cttcttcatc gattaaatac ttggtagtaa tcttcacctg gttgctgggt ccataggttt
     9961 ttacttcagg tgcctcgcca aattcattcg caagcagttc tcttatttcg ggcacattta
    10021 catcacggtc gaaacggact acataagtac gacctccggt aaaatctaca ccatagttca
    10081 aacccttgaa gataaacgag ccaatggcta aaaggaaaag ggcaatggag aaaatatagg
    10141 catattttct tattccaatg aaatcgaaat tagccttggt aaggaaacca cgagtaaatt
    10201 tattgtcgaa ttgaagcttg tagcccttat cggcccacca ttccataatg actctcgaca
    10261 ctaaaatggc cgtaaagaga gaggatatga taccgatgat gagggtggta gcgaatccct
    10321 gaataggacc tgagccaaag atgaaaagaa tgatacccgt tagcaaggta gttacgtttc
    10381 cgtcgatgat tgcggagtag gaattcttat atccatctgc tatggccact cgtaatccct
    10441 tcccagccct gacttcttcg agtattcgtt cgtagatgat aacgttggcg tcaacagcca
    10501 tacccattgt aagaacgata ccagcgatgc caggcaatgt aagcacggca ccaaatgagg
    10561 caagtactcc aaagatgaag aaaaggttga gacacagggc aagatcggca attaatccgc
    10621 ctcggttgta gtagaggaac atataaacca gaacgataaa aaatgctgca gcgaaggatt
    10681 tcattccggc gttgatagct tctttcccca atgagggccc cactacttct tcctgtacga
    10741 tgcgtgcagg tgctggcaaa cgtcccgact gcaggatatt ggccaggtct t
//
LOCUS       DAOORU010000077        10760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7649_length_10760_cov_6.133396, whole genome shotgun sequence.
ACCESSION   DAOORU010000077 DAOORU010000000
VERSION     DAOORU010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7649_length_10760_cov_6.133396"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="PLF20_06135"
     CDS             complement(<1..1524)
                     /locus_tag="PLF20_06135"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOX80616.1"
                     /translation="MKNYKRINNLLGWITFAIAFAVYMLTLEPTASWWDCGEYISTAY
                     KLQVGHPPGAPTFQLLGRLFTLVAGNDVTRVAYMINTMSALSSALTILFLFWSITMLA
                     RKVVLKQGQEINTAKLIAIMGSGFIGAMAYTFSDSFWFSAVEGEVYAMSSFFTALVFW
                     AILKWENVADEDHAVRWLIFIAFMIGLSIGVHLLNLLAIPAITYIYYYKKYKPNWKGF
                     LLTGVISIALLALIMYGIIPEIVNLFGKTELFFVNSLGLPFNSGTLFFALVLALMIVS
                     GLLYTIKGDKYKNFFLITLIIFFLILIGSGKGFLGVVISLAVAAISIYVVLKLKKARP
                     VINAVILSLAFILIGYSSFLMLVIRANANTPINENAPTDAIGLLSYLNREQYGSWPIF
                     YGQYYNTPVVDYKDQPPIYQKDEKSGKYVIADPRKGTLPVYDKRFTTLFPRMWSNQKQ
                     SHINAYQNWGKIKGTPIAVTSSSGKTETLIKPTYGENLRFFFSYQLGHMYFRYFMWNF
                     "
     gene            1715..1785
                     /locus_tag="PLF20_06140"
     tRNA            1715..1785
                     /locus_tag="PLF20_06140"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1747..1749,aa:Gln,seq:ttg)
     gene            1975..3423
                     /locus_tag="PLF20_06145"
     CDS             1975..3423
                     /locus_tag="PLF20_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOX80617.1"
                     /translation="MNKIALQAVVELFERYKGAKPSSVHHLPPSASERKYFRLNVEND
                     SILGAWNPCIQENEAFFSFTYQFLNKDLPVPPIYAISDDRKAYLLRDLGNVTLFSHLQ
                     ELRKSMSWNEQLTEIYRQTLDALIRFQLAGKGFDFSKAYPRGCFDEQSILWDLQYFKY
                     YFLKLAGIFFDEQALETDFRSFAEFLTQAPSGYFMYRDFQSRNVMLHDDTLYFIDYQG
                     GREGPCSYDLASLLYDAKADIPHPIREILLRYYIEQMKQADSLDPEEFLSYWPGFVYI
                     RILQALGTYGYRGFFQQKTHFLLSIPYALNNLSYVLENYGFAKQMPELERVVRVIAND
                     QTFRSFGKQTQRLLVQIVSFSYRKGIPLDSSGNGGGFVFDCRALPNPGIYQQFSKFTG
                     NHTEVIEFFDKFPEIKEFIQNCTALIQQSIHSYLEKGYTQLLVCFGCTGGQHRSVYCA
                     NALAEKISASNDYDVVLTHRELSFDKAIFMDE"
     gene            3495..4208
                     /locus_tag="PLF20_06150"
     CDS             3495..4208
                     /locus_tag="PLF20_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOX80618.1"
                     /translation="MNALILAAGRGTRLYPLTLRRPKALIEVGGISLLERAILKLSNE
                     GFQRIIINVHHFGDQIIAYLQAHAWPNVEIIISDEREELLDTGGAIKHAGKFLEGAPF
                     LVYNVDVVTDLKLMDLCRFHNNNNPLATLAISQRPTARQLYFDERMLLKGRAIMKPAD
                     EALTHAVDVFRTFAFSGIHVVSEKIFDLMPSQDVFSIMDFYLEFCKSYPIMGYDHTGI
                     FWMDLGTIENLQLFENLSA"
     gene            4271..7105
                     /locus_tag="PLF20_06155"
     CDS             4271..7105
                     /locus_tag="PLF20_06155"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOX80619.1"
                     /translation="MAAFLSLLADYIYEQYGNDFRNLCVIFPNRRAGLFFRRELGSKI
                     DRPVLSPQIFSFEDFIFEVTPFIQAEPFEKLITLYKAATNNPVLQSRDFDGFLDWGQN
                     VLRDFDEVDAYLIDPARLFPYLRNVKQLATWNLGETPPTEFQNNYLRFYDSMFSLYME
                     YHRLSMKAKKVSTAMAARWLAENSNFAELFKKWDHIVFAGFYANTPAEEKIISALKDS
                     GKAEIFYDADLYYIKNKLQEAGLFLRDKIDPEKDKWILNRLRDENREIKIIGVAGNVS
                     QTTALQKELEEKLKNEGTAWLDHAAIVLANEDLLIPVLDALPLEIEEVNVTMGLPLIA
                     TPVKQLVENFLNIFIQKKDDALPARLFYRLIHHPYIWAEMIDHFHEVNALRKKIMAGQ
                     AYLSHQQLNDVFKDNDTANQLVSLFFEVNNSIDIGNSLLALIDLVKDKFEKGLPQQYL
                     YVFSHILYGTLNKLKESDIDTNPQSFLKLMKRILASTKVPFYGEPLKGLQIMGMLETR
                     ALDFDTVFLLSANDDILPGSNRGNSFIPFDVRIEKEFNLPVDAHQSAISAYHFYRLLQ
                     GCKQAFMFYNSEPGGLGGGEKSRFILQIENEFPLNVKNTKISTTTVSFRYPSIPHEEI
                     VFTKTPEVMKKLTYKAQDGLSPSALWTFNVCGVKFYFDYMVGLIDYQITEGEIDEAVF
                     GTVVHKVLQEFYPHEYPFRVTEEFINNKLTQLDDLIRKSFQSELEGIDIDYGRNKLLF
                     GVAQQILTRFFQNEIEFLKDYPDLQILNLEKVITYTMPFPLSVDMDGSDISLDSVVIH
                     GRIDRIDQVGEQIRIIDYKTGKGLDKKLSLDISKNLSNKLDTDKAFQLLTYLWMYKMN
                     NPKLNDVKAGIMFLRRSDIGFTEAIIKNPSHSNDNPVQFNSSIQSIQYIEQQIKILLR
                     NLFDLNQSFVQTSNKNACRYCKYKFICARG"
     gene            complement(7109..8614)
                     /gene="amrB"
                     /locus_tag="PLF20_06160"
     CDS             complement(7109..8614)
                     /gene="amrB"
                     /locus_tag="PLF20_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOX80620.1"
                     /translation="MKLTNFLLIFTVFWMTTVGCHSQNKGGNMQSIDRKPAVAGQFYP
                     ADSASLQKSLENLFVQAKPGPTLNLRAIIVPHAGYIFSGKVAASGFNQIPADMKYENI
                     FIIGPSHRAAWDGASVYDRGDYITPLGKATVNTQLASELIKKYSFFSYRQDADAYEHD
                     IEVQIPFLQKHLKNPFKIVPIIIGTDSPETLKKIASALKPWFNPNNLFVISTDFSHYP
                     SFEDAVNVDRVTAQAICTNSPQNLLDTLISNRKKHISGLSTSLCGSSAVFTLLYITFE
                     LPGIEIKDIDYQNSGHSPYGDQDHVVGYHAMTITQKLSSNDFSLTDTDKKNLLKIARQ
                     SIEQYFKTKTRLPKPDALLTPNLLKPCGAFVTLHKHGELRGCIGHFGEDKPLWETVAE
                     MALASAFNDTRFYPLSEEELREIEIEISVLTPMRKISSINEIQLGKHGIYIKKGWRSG
                     TFLPQVATETGWTLEEFLGHCSRDKAGLGWDGWKDAEIYIYEAIVFSEKEF"
     gene            complement(8755..9825)
                     /gene="serC"
                     /locus_tag="PLF20_06165"
     CDS             complement(8755..9825)
                     /gene="serC"
                     /locus_tag="PLF20_06165"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HOX80621.1"
                     /translation="MKKHNFNAGPSILPRVAIENTAQAILDLNGSGLSVLEISHRSKD
                     FQAIIDEAVALFKELLHIPEGYHVLFLGGGASLQFCMVPFNLFNKKAAYLETGVWAKK
                     AIKEAKLFGEVVIPASSSDKNFNYVPKGFTIPTDVDYFHITTNNTIYGTELREDLDSP
                     VPLVADMSSDIMSRPLDVSKYAIIYGGAQKNVGPAGVAFVIIREDVLGKVERNIPTML
                     DYRTHIKEGSLFNTPPVLPIYTLMQTLRWLKGLGGVSEMEKINKQKANLLYDYIDQSK
                     MFVGTAEKDSRSLMNVCFVMKPQYTDKEEAFMDFAKNKGMVGIKGHRSVGGFRASLYN
                     ALPIESVKALIACMEEFEKANA"
     gene            10022..10723
                     /locus_tag="PLF20_06170"
     CDS             10022..10723
                     /locus_tag="PLF20_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275865.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOX80622.1"
                     /translation="MENKIPESIILAIGLLLLGSMIKGGIKVFSERDRMVSVKGLSEM
                     EVQANRVIWPLLFKEVGNDLLVLYDNLKDKNEAIVSFLKKNGIKDEEISISAPQVIDM
                     EADRYRAVPSPYRYNITSVITVMSDNVNLVRRLMSEQGELLQKGIAVGGDEYQYNVQF
                     LFTGLNDIKPRMIEEATKNARAAAEKFARDSNSKLGKIKSANQGQFTISDRDANTPYI
                     KNIRVVSTIDYYLKD"
BASE COUNT         3132 a         2023 c         2228 g         3377 t
ORIGIN      
        1 aaaattccac ataaaatatc tgaaatacat gtgccctaat tgataactga aaaagaacct
       61 cagattttca ccataggtcg gttttattaa ggtttcggtt tttccgctgc tactggttac
      121 tgcaatgggt gtgcctttta ttttacccca gttttgatag gcattgatat gactttgttt
      181 ttgattgctc cacattcgtg gaaacaaggt cgtgaatcgt ttatcgtata caggcagagt
      241 accttttcga ggatcggcaa tcacatattt tcccgatttc tcgtcttttt gataaatagg
      301 tggttggtct ttataatcta caacgggtgt attgtagtat tggccataaa agataggcca
      361 tgagccatat tgttctctat ttagatacga aagaaggcca atggcatcgg ttggagcatt
      421 ttcgttgata ggtgtattgg cattggcacg tataaccagc attaagaatg aagaataacc
      481 tataagaata aatgctaacg ataaaatgac cgcattaata acaggccgtg ctttcttcaa
      541 tttaagaact acatagatac taattgctgc aacagccaat gagatcacaa cgcctaaaaa
      601 tccctttccc gatccgatta aaatcaaaaa aaagataatg agggtaatta ggaagaaatt
      661 tttatacttg tcgcctttga tcgtataaag taagcctgaa actatcatta aggcaagcac
      721 taatgcaaag aaaagagttc ccgagttgaa tggcaatcca agtgaattaa cgaagaacaa
      781 ttcggttttg ccgaaaaggt taacgatttc gggaatgatt ccatacatga tcagtgccag
      841 taaggctata gaaatgactc ctgtaagcag gaatcctttc cagttgggct tgtatttctt
      901 gtaataatag atataagtta tagcaggaat agccaagagg ttcagtagat gtactccaat
      961 ggaaagacca atcatgaatg ctataaagat aagccagcga acggcatgat cttcgtcagc
     1021 cacattttcc cattttaaga ttgcccagaa gacaagagcc gtgaagaaag acgacatggc
     1081 ataaacttct ccttcgacag ccgagaacca gaatgagtcg ctgaaagtat aggccatagc
     1141 tccaatgaat cccgagccca tgatggcaat taattttgct gtattgatct cctgaccttg
     1201 ctttaaaacc acctttcggg caagcatcgt aatgctccaa aaaaggaaca ggattgtcag
     1261 agcactgctt aaagccgaca tggtgttgat catataagct actcgcgtaa catcattgcc
     1321 tgctacaagg gtaaataaac ggcctaataa ttggaaagtg ggtgctcctg gtggatgccc
     1381 aacctgtaat ttgtatgctg ttgaaatgta ttctccacaa tcccaccagc tggctgtggg
     1441 ttcgagagtc agcatgtaaa ctgcaaaagc aatggcaaag gtgatccatc caagcaggtt
     1501 gttgattcgt ttgtaatttt tcatgttttt actgattaaa ttcaaagtat cagtattttt
     1561 ttccgactga ttgtctatcg attaaaccat taaacgcttt gaaattttaa tattatgaat
     1621 cgagtgcgaa agtaataaaa aatttatggc ttgtaaaatt atgtggcata caacttgaac
     1681 agaggatttt ttttatcttt gccgccgaaa attttgtccg atggtgtaat ggcagcacag
     1741 gagattttgg ttctcttagt ctaggttcga atcctggtcg gacaacaagc cccggtgaat
     1801 gcggggctca ttttttaata tgtctatcct aagctttgag agaggatttt tgctattctt
     1861 attaactatt tgttattcat tattttatag attgatttgc tatttcattc ctctttaatg
     1921 atatctcatt cgaattttgc tgattacttt tgtcatataa attgaatttt ttcattgaat
     1981 aagattgcat tacaggctgt tgtagagctt ttcgaaagat ataagggtgc gaaaccatct
     2041 tccgtacatc atttgcctcc ttcggcttcc gaacgtaaat attttcgatt gaacgttgag
     2101 aatgactcca ttcttggagc ttggaatcca tgtattcagg aaaatgaggc ttttttttct
     2161 tttacctatc agttcctcaa taaagacctc cctgttcctc ctatctatgc tatctctgat
     2221 gaccgcaaag cctacctctt gagggatttg ggtaatgtta ctcttttcag tcatttgcag
     2281 gaactacgaa aatccatgag ctggaatgaa caacttaccg aaatttaccg gcaaacctta
     2341 gatgctctca ttcgatttca gcttgcaggt aaaggtttcg atttttcgaa agcctatcca
     2401 cgtggctgct tcgatgagca aagtatctta tgggatttgc agtatttcaa atattatttt
     2461 ttgaaacttg ctggaatttt tttcgacgag caggcactcg aaacagattt tagatctttt
     2521 gctgagtttt tgactcaggc tccttcggga tatttcatgt accgcgattt ccagtcgcga
     2581 aacgtcatgc ttcatgatga tacattatat tttatagatt atcaaggtgg aagggaaggt
     2641 ccttgctcat atgatctggc ttcacttctt tatgatgcca aagccgatat accccatcct
     2701 atccgtgaaa ttctacttcg ttattacata gagcagatga aacaagctga ttctcttgat
     2761 cctgaagaat ttcttagcta ctggcctggc tttgtgtata tcagaatcct tcaggcattg
     2821 gggacttatg gttatcgggg atttttccag caaaaaactc atttcctcct cagcatacct
     2881 tatgccttga acaatctgtc ctatgtcctc gaaaactatg gctttgccaa gcaaatgcca
     2941 gaactggaaa gagtggtaag agtaattgct aatgatcaaa cattcagatc ttttggcaaa
     3001 caaactcaac gattgctggt acaaatcgta agtttttcgt acagaaaagg tattccactc
     3061 gactcctcag gcaatggtgg tggttttgtt ttcgattgca gagccttgcc taatccgggt
     3121 atatatcagc aattttcgaa atttacagga aatcataccg aagtgatcga atttttcgat
     3181 aagtttcctg agataaaaga gtttattcag aattgtactg cactgattca gcaaagcatt
     3241 cattcctatc tcgaaaaggg ttatacccag ctgttggttt gttttggatg tacaggcggt
     3301 cagcatcgtt cggtttattg tgccaatgca ttggccgaaa aaatttcggc ttctaatgat
     3361 tacgatgttg tactcaccca tcgtgaactt tcttttgata aggcaatttt tatggatgag
     3421 taaatttgtt ctattaatga aatgagtgaa taaaaaacca aatattttaa aattttctaa
     3481 tcgagttcaa tataatgaat gccttgattc ttgcagccgg aagaggaaca cgcctttatc
     3541 ctttaacact tcgtcgtccc aaggctttga tagaagtggg cggaataagt ctgctcgaaa
     3601 gggctatcct aaaattatcg aatgaaggtt ttcaaagaat tattatcaat gttcatcact
     3661 ttggcgatca aataattgca tatttgcagg ctcatgcatg gccgaatgtt gagattatca
     3721 tcagcgatga aagagaagaa ttactcgaca caggcggggc gatcaagcat gccggtaagt
     3781 ttctcgaagg cgctcccttt ttggtttata atgtagatgt tgttaccgac ctgaagttaa
     3841 tggacctttg tagattccat aataataaca accctctggc aactcttgct ataagccagc
     3901 gtccaacggc aaggcagttg tattttgacg agcgaatgtt actgaagggc agagcaatta
     3961 tgaaacctgc agacgaagct ctgactcacg cagttgatgt ttttcgaaca tttgccttta
     4021 gtggaatcca cgttgtaagt gaaaagatat tcgacctgat gccatcacaa gatgttttct
     4081 ctataatgga tttttacctt gaattctgca aaagttatcc catcatgggt tacgatcata
     4141 ctggaatttt ctggatggat ttaggcacca tcgaaaattt acagcttttt gagaatcttt
     4201 ctgcttagca aaatattttc atcgattttt ttatggcttt agattatttt cgcaacttaa
     4261 tacgaatata atggcagcat tcttatctct tctcgcagat tatatttacg agcaatatgg
     4321 gaacgatttc aggaatcttt gtgtaatatt tcccaatcgg cgggcgggat tattctttcg
     4381 cagagaactt ggttcaaaaa tagatagacc tgttttgtct cctcaaattt tttctttcga
     4441 agatttcatc ttcgaggtta ctccttttat tcaggctgaa cctttcgaga agttaatcac
     4501 cctgtataag gctgcaacaa ataatccggt gcttcaatca agagatttcg acggttttct
     4561 cgactggggt cagaatgttt tgagagattt tgatgaagtg gatgcctatt tgattgatcc
     4621 agccagatta ttcccctatc tgcggaatgt aaagcaattg gcaacatgga atttgggaga
     4681 aactcctcct acagaatttc aaaataacta tctgcgtttc tatgattcca tgtttagtct
     4741 ctatatggaa tatcatcgcc tgtcgatgaa ggcgaaaaag gtttcaacag ctatggctgc
     4801 tcgatggtta gccgagaatt caaattttgc cgaacttttt aagaaatggg atcatattgt
     4861 gtttgctgga ttttatgcaa atacaccggc agaagaaaaa attatttctg ctttaaagga
     4921 ttcaggtaaa gccgaaattt tttacgacgc cgacttgtat tacattaaaa ataaacttca
     4981 agaagctggc cttttcctta gagataaaat cgatccagaa aaggataaat ggattctcaa
     5041 tagattacga gatgaaaaca gggaaataaa aattattggc gttgcaggca atgtgagtca
     5101 gacgacggca ttacaaaaag aattggaaga aaaactcaag aatgagggaa ctgcttggct
     5161 cgatcatgcc gctattgtat tggccaatga agatttgttg attcctgtgc tcgatgcttt
     5221 acctcttgaa atcgaggagg tgaacgttac aatgggttta ccacttatag ccacgcctgt
     5281 aaaacaactt gtcgagaatt ttttaaacat cttcatccag aagaaagatg atgccttgcc
     5341 agccaggcta ttttatcgcc ttatacatca tccatacatt tgggctgaaa tgatcgatca
     5401 ttttcatgaa gtaaatgctt taagaaaaaa aatcatggct ggccaagcct atttatcaca
     5461 tcagcaactg aatgatgtgt tcaaggataa tgatactgca aatcagctcg tttccttatt
     5521 ttttgaggtg aataattcaa tcgatatagg aaattcgctc ttagccttga ttgacctcgt
     5581 taaggataaa tttgagaagg gccttcctca acaatatctc tatgttttca gccatatttt
     5641 atatggaaca ttgaacaaat taaaagaatc ggacatcgat accaaccccc aatctttttt
     5701 gaagctaatg aaaaggattt tggcttccac caaagttcct ttttacggtg aaccattgaa
     5761 gggattacag attatgggca tgctcgaaac ccgtgcactc gatttcgata cggttttttt
     5821 gttgtcggcc aacgatgaca tcctgcctgg tagcaaccgc ggtaattcct tcattccttt
     5881 cgatgtaaga atagaaaaag aattcaatct gcctgtcgat gctcatcaat cggctatttc
     5941 ggcctatcat ttttaccgtt tgcttcaggg ctgcaagcaa gcctttatgt tttacaacag
     6001 cgaacccggg ggtttgggcg gaggtgaaaa aagtcgcttt atccttcaaa tcgaaaacga
     6061 attcccttta aatgtgaaaa atacgaaaat ctcaaccacc acggtgagct ttcgctaccc
     6121 cagcattcct cacgaggaaa tagtttttac taaaactccc gaagtcatga aaaaattgac
     6181 gtataaagct caagatgggc tttcgccttc ggctttgtgg acttttaacg tttgtggtgt
     6241 aaaattttat ttcgactata tggtgggttt gatcgattat cagattaccg aaggagaaat
     6301 tgatgaagct gtttttggga cggtggttca taaagtttta caggaatttt atcctcatga
     6361 gtatccattt cgtgtaactg aagaatttat aaataataaa cttactcaat tagatgattt
     6421 aatcaggaag agtttccaga gtgagctcga aggcatagat atcgactatg gacgtaataa
     6481 gttgcttttt ggcgtggctc aacaaattct tacaagattt tttcagaatg aaatcgagtt
     6541 tttaaaggat tatcccgatt tgcaaatttt gaatcttgaa aaggtaatca cttataccat
     6601 gccttttcca ttatcagttg atatggatgg tagtgacatc tctttagatt cggtagtcat
     6661 tcatggccga atcgatcgca tcgatcaagt aggcgagcaa attcgcatca tagactacaa
     6721 aacaggcaaa ggcttagata agaaattgtc tttagatatt tcaaaaaatc tttccaataa
     6781 attagataca gacaaagcct ttcaattgct tacttattta tggatgtata agatgaataa
     6841 tccgaaattg aatgatgtga aggctggcat aatgtttctt agaagatctg atattggttt
     6901 tactgaggcc ataataaaaa atccttccca ttcaaatgat aatcctgttc aatttaattc
     6961 ctctatacaa tctatacaat acattgagca acaaataaaa atcttgctta gaaatctttt
     7021 cgaccttaat caatcctttg tccagacttc aaacaagaat gcctgtcgtt attgtaaata
     7081 caagtttatt tgtgcccgcg ggtgaagatt aaaactcttt ttcgctgaaa acgatagctt
     7141 cataaatata gatttcggcg tctttccaac cgtcccaacc caagccagct ttatcgcggc
     7201 tacaatggcc aagaaattct tcgagtgtcc agcctgtttc ggttgctact tgaggcaaga
     7261 atgtacccga tctccagcct tttttgatgt agatgccatg ttttcctaat tgaatttcgt
     7321 tgatggatga gattttgcgc atgggtgtaa gcaccgaaat ttctatttca atttctctga
     7381 gttcctcttc gcttaaggga taaaatcgcg tatcgttgaa agccgatgca agagccattt
     7441 cggctacagt ttcccacagt ggtttgtctt ctccaaaatg gcctatacag cctcttagtt
     7501 ctccgtgttt atgaagtgta acaaaagctc cgcagggttt cagcagattc ggggtaagaa
     7561 gtgcatcggg ttttggcagt cgcgttttcg tcttgaaata ttgttctatg ctttgacgtg
     7621 caatcttcaa tagattcttt ttatcggtat cggtaagcga aaaatcgttc gaactaagtt
     7681 tttgggttat ggtcatggcg tgatagccta ccacgtggtc ttggtcgcca tagggactgt
     7741 gacctgaatt ttgatagtcg atgtctttga tttcgattcc gggtaattcg aaagtaatgt
     7801 aaagcagtgt gaaaactgca gaagagccac agaggcttgt cgaaagcccg gagatatgtt
     7861 ttttacgatt cgaaatcaag gtatcgagaa ggttttgggg agaattggta caaatagctt
     7921 gtgcagtgac cctgtccaca ttcaccgcat cttcgaatga aggataatgt gaaaaatcgg
     7981 tcgaaattac aaaaagattg tttgggttga accatggttt taatgctgac gcaattttct
     8041 tgagggtttc gggtgaatcc gtaccaatga tgattggaac gattttgaat ggattcttaa
     8101 gatgtttttg caggaaagga atctgaacct cgatgtcgtg ttcataggca tcggcatctt
     8161 ggcgataaga aaagaaagaa tattttttta tgagttcgct ggccaattga gtgtttactg
     8221 tagctttgcc taagggggta atataatcgc ccctgtcgta aacgcttgct ccatcccagg
     8281 ctgcgcggtg gcttggccca atgataaaaa tattttcgta tttcatgtcg gctgggatct
     8341 gattgaaccc cgatgcagct acttttcccg agaaaatata gcctgcatga ggcactatga
     8401 tggctctgag attgagagtt gggcccggtt ttgcctgtac aaacaagttt tcgagacttt
     8461 tttgcagtga agcactatcg gccggataaa actgacccgc cacagcaggt ttacgatcga
     8521 tgctttgcat atttcctccc ttattttgtg aatgacaacc cacggtagtc atccaaaaca
     8581 ctgtaaatat caatagaaaa ttagtaagtt tcatgttttg gagtctattt aaaataggaa
     8641 tgctaattta gtataaaaaa ctgtaaattt tccgagcaat gaaaattttt atacaaaaca
     8701 gaaaaaccgt actgaaagag tacggttttc taattattct tttcgagagg gcattcaggc
     8761 atttgccttt tcgaattctt ccatgcaagc aatcagagct tttacacttt cgatgggcaa
     8821 agcattgtat aaggaggctc taaaaccgcc tactgaacgg tgacctttaa ttccaaccat
     8881 tcccttgttt tttgcaaagt ccatgaaggc ttcctccttg tcggtatatt ggggtttcat
     8941 tacaaagcat acattcatga gcgaacggct gtctttttcg gctgttccca cgaacatctt
     9001 gctctggtcg atgtagtcgt aaagcagatt agccttttgt ttgttgattt tttccatttc
     9061 gctcactccg cccagccctt tgagccagcg caaggtctgc ataagggtgt aaataggtag
     9121 aactggagga gtattgaata acgatccttc cttgatgtgt gtacgataat cgagcatggt
     9181 gggaatattg cgttccactt tgcccagaac atcttcacga attataacga aagcgactcc
     9241 tgcaggaccg acgtttttct gtgcacctcc atatatgatg gcatattttg aaacgtccaa
     9301 aggcctgctc atgatgtcgg acgacatgtc ggcaaccaga ggaacggggc tgtcgaggtc
     9361 ttcacgaagt tcggttccat aaatcgtgtt gttggtagta atgtggaagt aatctacatc
     9421 ggtgggaatg gtaaaaccct tggggacata gttgaaattt ttgtcgcttg acgaagcagg
     9481 tatgactact tcgccaaaga gtttagcttc tttgatggct tttttggccc atacgcctgt
     9541 ttcgagatag gctgctttct tgttgaagag gttgaatgga accatacaaa actgcaagga
     9601 cgcaccccct cccaggaaca gtacatgata tccttcggga atgtgcagta gttctttaaa
     9661 cagagccaca gcctcatcga tgatagcctg aaagtcctta ctgcgatggg agatttccaa
     9721 aacactcaaa ccagacccgt tcaagtcgag tatagcctga gccgtgtttt cgatggcaac
     9781 ccgcggtaat atagaaggtc ccgcattaaa attatgcttt ttcatagttt tgaattttta
     9841 agcagatgat tcacccgcat gaaatatttt tactgcaaag gtatgggtat atttccaaat
     9901 attcaagtaa aaatcggaaa caaatatttc atttgctgac atcgtttctt tctctccact
     9961 ttatgatttt gttgataaat cccttatttt tgttgattca tctcattaaa taaattgaaa
    10021 catggaaaat aagattcctg aatccattat tctggctata gggctgcttt tgttgggctc
    10081 gatgatcaaa ggaggtataa aggttttttc ggagcgcgac cgtatggtaa gcgtaaaagg
    10141 cctttctgag atggaagtgc aagctaaccg tgtaatctgg ccccttcttt ttaaggaagt
    10201 aggcaatgat ttattggttt tgtacgataa tttaaaagac aaaaatgaag ctattgtttc
    10261 ttttttgaag aaaaatggca taaaagacga agagataagt atttctgctc ctcaagtcat
    10321 cgatatggaa gccgatcgct accgagcggt cccttctcca taccgttata atattacttc
    10381 ggtgataaca gtcatgtcgg ataatgtaaa tctggtgcgc cgattgatga gtgaacaggg
    10441 agaattgctc cagaaaggaa ttgccgtggg aggagacgaa tatcaataca atgtgcagtt
    10501 tctgtttacc ggtctgaacg atatcaaacc tcggatgata gaagaagcaa caaagaacgc
    10561 gcgtgcggca gctgaaaagt ttgccagaga ttccaacagc aaactcggga aaattaagag
    10621 tgccaatcaa ggtcaattca ctattagcga ccgcgatgcc aatacccctt atattaagaa
    10681 tattcgtgtg gtaagcacca tcgattatta cttgaaagat taaacttttt tgatattgag
    10741 gtcgatagag agaggggtgt
//
LOCUS       DAOORU010000078        10694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7709_length_10694_cov_4.631356, whole genome shotgun sequence.
ACCESSION   DAOORU010000078 DAOORU010000000
VERSION     DAOORU010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10694
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7709_length_10694_cov_4.631356"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..336)
                     /locus_tag="PLF20_06175"
     CDS             complement(<1..336)
                     /locus_tag="PLF20_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOX80623.1"
                     /translation="MKNIELVFATNNPHKLREASQILGPNIEILMLNEIGCFDEIPED
                     GATVEANASIKSWYVYRHYQKNCFADDTGLEIDTLGGRPGVFSARYAGENKSFDDNIR
                     KVIYEMKGKT"
     gene            complement(388..1149)
                     /locus_tag="PLF20_06180"
     CDS             complement(388..1149)
                     /locus_tag="PLF20_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HOX80624.1"
                     /translation="MSKNFIWSIVFFILMIREGGLFAQPEIWCLPEYSEDELVIRHVG
                     FTLKYDKNHKLSDWVAYDIVPYKLLKSVKKSNHFIPDPQLPDEWSAHSIDYKYSGYDR
                     GHLAPAADMAWSAQAMKESFYLSNICPQQPQFNRITWKKLEEKVRQWARIYDTLLITT
                     GPVLSSDLPKIGHGKVSVPKYFYKAVVIYTHHKKSGIAFVMQNSYSSKVLMQSAITID
                     SLERLTGFNFFARLPEEEQLNIEGKLIIEDWNILR"
     gene            1719..1868
                     /locus_tag="PLF20_06185"
     CDS             1719..1868
                     /locus_tag="PLF20_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80625.1"
                     /translation="MKDKIKIMKEKIALILLFVFLIGMIASCKSEERCAAYGEAKKYQ
                     VEKRQ"
     gene            complement(2006..3484)
                     /locus_tag="PLF20_06190"
     CDS             complement(2006..3484)
                     /locus_tag="PLF20_06190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80626.1"
                     /translation="MKRIFTFFFLLIGFSVIAQPKIYTPTLLLPNNGATNQMPDVTLS
                     WSAVTGLGVIKYKLEIDDNPDFTSPKVFYTEFLTGYTMSNLHYGVQYHWRVKAYDLAT
                     NDSSYWSATRNFTVFSKVDNKKPENKTKEITPLATLEWKDRLGPNLVTGNTYFNYILD
                     TVETFDSPMAYLGTVSGSVYKATTPKMRFGTRYFWKVRAINEKDTSNWSDAWYFRTLD
                     TIVLIDPANNATNQQINLTLSWNAANGVTKYDYQICTNPEFDEPLHFITDKTSVIPSG
                     ITFGGHYYWRVRGRHTSDTSSWGVTRSFFIIPYPILKSPANGDTNVSTQPLFSWEPMT
                     GIESYYLQYDKDPNLTDGFISPPIGDSLSNYRCPYILEENTVYYWRMRATANSDTSQW
                     SPVFSFTTLKGIGMIENDLDKISIFPLPANNYLNIRFPSTMSGQATIQLIDLVGQKVI
                     ELTDEINSQNPYKLNVSNLKSGIYILRISNHGNSFIRKVSIE"
     gene            complement(3516..6305)
                     /locus_tag="PLF20_06195"
     CDS             complement(3516..6305)
                     /locus_tag="PLF20_06195"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80627.1"
                     /translation="MKNKSLLFFLTALMMGCGSYLFASGIAGSKGNEGDRPGKAQLQN
                     HLKSGNAVSLNWQSLGPDNVGGRTRAILFDKRDATFNTVYAASVAGGIWKSTNLGSTW
                     VKVNQANSNLNVSCMAQTDNNDIYAGTGEYGFSQGTGLYVSTDGDNFSLVPGTMPTMV
                     DNVLEWAYINELAIQNGRIYAATNAGLKYRNSDESQWRTANYIDGEGQLQPLNGFVWD
                     VKVGSNGIVVASIESKVYVSELGQPNGFINQSTKQVLPDTVLNPEKLPSIGLGRIELA
                     VAPSNPDYIYACASDPYGYFDNIYRSIDKGITWEIIFPGHSSLLPDIFVVNNVAYGLV
                     ANTIVVYPNNPEEILVGGINMWHGKFINEGYYYWGTDAISSYATSKFSPLYLHELHHT
                     YVFKPNDPKTFLIGTDGGISTNIDGELYFKSLNRTYMTSQFVTLNVNGFGNPIGGTLG
                     NGIQYIGDGTNSPIGAVEVWNGDFNNSGEGGYVAISKINPKVFILSLKGGPFRRTEDK
                     GFSFSTTFLNSGMSAPANTYCPMILWESFNDPTSMDSTRFKASKDITEGEEIIVRSQI
                     YDYPFKTIAPHDIHKGDVVYFKDPLQAKTFLALGDKVYYTKEILDFTKEPEWWQVAKV
                     VGTVSCMSLSADANHLYVGTESGNVFRISNLTHANSAATASITSATCVVATQQIKSFP
                     NRIVTSIAVDPKNPQHILVTLGNYGFDEYVYNVTNALDSLPKFTSVQTNLPHGPVYSS
                     LIELNNSNLIILGTEYGIFLTNNISAPNWVNDNGPMGRIPVKVLLQQTVNGGGIFVPS
                     DDPNIPGVYYPEITNYGVIYAATYGRGVYSSNFFVGIDEPNIDTPPTNAQLAIYPNPV
                     RNELSFTLKGDYASEVFIEIYDFSGRKSKENHLKIQPGSNTFTMDVSNLKRGTYILRC
                     ISDTSISTGKFIIVK"
     gene            7008..8444
                     /locus_tag="PLF20_06200"
     CDS             7008..8444
                     /locus_tag="PLF20_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HOX80628.1"
                     /translation="MRTEKDFMGELQLPDDALYGIQSLRARKNFPHHSHFFEEWYRAM
                     GIVKHACYLTYLSFCRAVQQKNLESRLPSPLISSEIMDGLVQASIEVSRGEHFEHFIV
                     PGISGGAGTSINMNINEIIANRALIISGHSIGDYHYIDPILHANIYQSTNDTVPTALK
                     IAMLNLLGQLEQEINLLRAETERLETASRNILRVGRTQMQDASPSSYGILFSTYSEAF
                     SRDWWRMSKCFERIKSVNLGGSVIGTGIGVPRFFIVEVITHLQTLTGLPLSRSENMPD
                     ATANLDSLAEVHGIIKAHAINLEKIANDIRLLSSDIASPGEISIPQKQIGSTIMPGKA
                     NPVIPEYVISCVHHVYANDEVITSLVASGCLDSNAYLPIIGHCLIDSLKTLISANKTL
                     KDNLLNDLKFHPTVAAHKTWNSPAITVALSPFIGYYKASQLACEMKNTHCDVFTANQR
                     LHLINPDKLKEIMRTENLLKLGYSLNEL"
     gene            8456..9685
                     /gene="hydF"
                     /locus_tag="PLF20_06205"
     CDS             8456..9685
                     /gene="hydF"
                     /locus_tag="PLF20_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOX80629.1"
                     /translation="MIKGKEARLHIGIFGRRNNGKSTFINCLSGQELSIVSDIAGTTT
                     DPVTKAMEIGGLGPVVFIDTAGIDDSGPLGQKRVQKTLEILKTIDLAILLIAENAFGE
                     QEESIIRQFKEWDVPFMIIHSKEDLIPMNKQTLSWAKNQQPIAIETFSPSDSNHLPAI
                     IQAISKSIPESAYKSRSLVGDLLNYGDVVMLITPIDIEAPEGRLILPQVQVIRDILDN
                     DAIAVVVKEREADAYIRKMSPQPALVITDSQVFTKADAIVPRNIPLTSFSILLARQRG
                     DFQNYLKGTPKISQLKDGERVLLLESCTHQVSCDDIGRVKIPRWLSNFTGKKIEYEVV
                     SGLDSLPRPITDYALIIQCGGCMITRRQIINRLKPAVEAGIPITNYGMAIAWVQGIYP
                     RAIAPFTGQTVNYSDYL"
     gene            9705..>10694
                     /locus_tag="PLF20_06210"
     CDS             9705..>10694
                     /locus_tag="PLF20_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80630.1"
                     /translation="MKIPTISFFTIGIFLLFPPPLSFSQSATLGPVSLSLKKGIVLRS
                     FTDTKNRALTPSTLIPFFAFDIDDQVVDPQEFKCLQQGDSLYFRTDKMEGSMVYINYD
                     KGAKFRLRIRNKDNVKHSVSNVLPFGKDSQSIYITGAGNFEWPYYLNRSQLFRPGYGP
                     IGVVLPDNAWHLGFSDIPLEISNKRLCGLARRAGISKGNFTRWHAELEPGGEVIYHLW
                     LDTHEGEWQNGLKLMFQERYLYDLDDFNNSLYERHDLSWIKSAWIILLQSAWDHEWYD
                     ALIHQSIYEETLSKYDRMVGGYDVFAIWPTWPRLGLDQRSQFELYEDLPGARIV"
BASE COUNT         3124 a         2091 c         2216 g         3263 t
ORIGIN      
        1 agttttcccc ttcatttcgt agattacctt ccggatgttg tcgtcgaatg atttattttc
       61 cccagcataa cgcgccgaga aaacccccgg acgtcctcct aaagtatcta tctccaatcc
      121 tgtatcgtcg gcaaaacaat tcttctgata atggcggtac acataccacg attttattga
      181 agcattggcc tcgaccgtag caccatcttc gggaatttca tcgaaacatc cgatttcatt
      241 tagcatcaga atctcaatat ttggtcctaa aatttgtgaa gcttccctta gtttatgtgg
      301 attgtttgta gcaaaaacga gttcaatatt tttcatttcc agattgttat tcgtctttta
      361 tcaatcatat atcatatatc ttgtatttta tcttaatata ttccaatctt ctatgatgag
      421 cttcccttcg atattaagtt gttcctcttc tggaagacga gcaaagaaat tgaatcctgt
      481 tagcctttcc agcgaatcaa tagtaatggc cgattgcatc aaaaccttgg aagaataact
      541 attttgcatt acgaaagcta ttcccgattt cttgtggtgt gtgtaaataa ctactgcctt
      601 ataaaaatac tttggcacac tcactttacc atgtcctatt tttggaaggt ccgagctaag
      661 gacaggacct gttgtaatga gcaaggtatc ataaatccga gcccactgcc gtaccttttc
      721 ttcaagcttt ttccacgtta tgcggttaaa ttgaggctgc tgaggacaaa tattactcaa
      781 atagaatgat tctttcattg cctgcgccga ccatgccata tcagcggctg gtgctaaatg
      841 accccggtcg taaccactat atttataatc tattgaatga gccgaccatt catcaggcag
      901 ttgtggatcg ggtataaaat ggttggattt tttcactgat tttagaagct tatagggcac
      961 tatgtcgtaa gctacccaat cggaaagttt gtgattcttg tcatatttca aagtgaatcc
     1021 tacatgcctg atgacaagtt catcctcgga atactccggt aaacaccata tttccggttg
     1081 agcaaataaa cccccttccc taatcattaa aataaaaaag actattgacc agatgaagtt
     1141 cttcgacatc aatacgaatt atgacttcaa aattacaaag ttttagcatt ttgatattga
     1201 tgaggccttg gatacttttg ttgaagctta ttctaattat gctatcctga taaaaacggc
     1261 aaaagcggtt tttaactgaa tgaatgatta gccgaggagg taatgttcat tcttcatatt
     1321 gtatagtcat tttacaagtt acaagcactt atttaggcca aatggcttcg gggtggggag
     1381 aatcttcatc tgaataaacg gttcgtttgc aattattttc gatggtgaaa gagtggtttt
     1441 ttacctgaaa tcatctcgat aaaggccgat tgaaagatag aaggaaatga aaacatatcg
     1501 acgagaaata gaaacagact tcgatgaatg ttgacttctc agcgttggag tgaaaaattt
     1561 accgaagatt aacaataaaa agttgaattt tatcataata agcatttatt ttgtagcctg
     1621 aaattttgaa tgaatgtccg tgctttaaag accgaatttt tttagtaaat tatacagtta
     1681 tagaaaattg aaacatgatc tctaaagagg caaacaagat gaaagacaag ataaagataa
     1741 tgaaagagaa gattgctctc attttattgt ttgtattcct aataggtatg atagcttcgt
     1801 gcaagtcgga agaaagatgt gcggcttatg gggaagccaa gaaataccag gtagaaaagc
     1861 gtcaatagat ttcggtgatt tagctatatt ttaagcaatg ctaataagag gaaagttatt
     1921 tatttcgaac acagggtggt ctaaaaaaaa gaaaagggct gttttcgatg cagccctttc
     1981 cttttttggt atcagaacat ttctgttatt ctatggagac ttttcggatg aatgagttcc
     2041 catgattaga aatgcgaagg atataaattc cacttttcaa attcgaaaca ttgagtttgt
     2101 aagggttttg tgaattaatt tcatctgtaa gctcgatcac tttttgtcca accaggtcga
     2161 taagttgaat tgtagcttgg cctgacatgg tagaggggaa gcgtatgttc aggtaattat
     2221 tagctgggag agggaaaatg ctgattttgt ccaaatcgtt ttcaatcatt ccaatccctt
     2281 ttaaggtagt aaacgagaat acaggtgacc attgagaagt atcagaattg gctgtagctc
     2341 tcattctcca ataatagacg gtattttctt cgagtatata agggcatctg tagtttgata
     2401 gggaatcacc tatgggaggg ctgatgaatc cgtcggtaag atttggatct ttatcatact
     2461 gtaggtaata agattctatc cccgtcattg gttcccatga aaagagaggt tgggtactca
     2521 cgttagtatc gccatttgct ggagatttca gtatgggata aggtatgata aagaatgaac
     2581 gtgttacgcc ccagctggaa gtatcggagg tgtgtcttcc tctcactctc caatagtaat
     2641 gacctccaaa ggtgataccc gatggaatta cgctggtttt gtcggttata aaatgtaaag
     2701 gttcatcaaa ttcgggattg gtgcatattt gatagtcgta ttttgtaacg ccattggctg
     2761 cattccatga caaagtcaga ttaatctgtt gatttgtagc attattggcg ggatctatta
     2821 aaacgatggt atccagggtc ctgaaatacc atgcatccga ccaattgctt gtatcttttt
     2881 cgtttatggc tcttaccttc cagaaataac gagttccgaa acgcattttg ggtgtagtgg
     2941 ctttgtaaac gctgccgctc actgtcccta aatatgccat aggactgtcg aaagtttcta
     3001 cggtatcgag gatatagttg aaatatgtat ttcccgtaac cagattaggc cccaaacggt
     3061 ctttccattc cagcgtggct aatggcgtaa tctcctttgt cttgttttcg ggtttcttat
     3121 tatctacttt actaaagaca gtaaagtttc gtgtagccga ccagtatgaa gaatcattag
     3181 tggccagatc ataagccttt accctccagt ggtattgtac cccgtaatga agattactca
     3241 tggtataacc cgtcagaaat tcggtataga ataccttggg agaggtgaaa tctgggttat
     3301 catcaatttc gagtttgtac tttattacgc ccaaacctgt aactgccgac cagctcaatg
     3361 taacgtcggg catttggttt gtagcaccat tattaggcaa aagcagggtg ggtgtataaa
     3421 tcttaggttg agctattacc gaaaacccga ttaatagaaa gaagaaagta aaaattcttt
     3481 tcattgttag atgctattta aaaattataa gacttctatt ttacgatgat gaacttaccc
     3541 gtcgagatag aggtgtcgga gatgcaacgc aggatataag taccgcgttt cagattggaa
     3601 acatccatgg taaacgtatt ggagcctggc tgaattttca gatgattttc ttttgatttt
     3661 cttccactga agtcgtagat ttcgatgaaa acctccgaag cataatctcc ttttagggta
     3721 aatgagagtt cgtttcggac gggattcgga taaatggcaa gctgggcatt agtgggtggg
     3781 gtatctatat tgggttcgtc gattccgacg aagaaattag agctgtaaac acctctcccg
     3841 tatgtagcag catatatcac tccataattg gtgatttcag gataataaac tcccggaata
     3901 tttggatcgt cagagggcac gaaaatacca ccaccattaa cagtttgctg taataagacc
     3961 ttgacaggaa ttctacccat ggggccattg tcgttcaccc agtttggagc ggaaatattg
     4021 ttggtaagaa agatgccata ttccgtaccc agaattatta aattggaatt gttcaattct
     4081 atcaaagacg aataaactgg tccgtgtgga agattagtct gaacagaagt gaatttgggt
     4141 aatgaatcta gagcattggt aacattatat acgtattcgt cgaatccata gttaccaaga
     4201 gttaccagga tatgttgagg atttttgggg tcgactgcaa tggaagtaac tatacgatta
     4261 gggaaagatt tgatttgttg agtagctact acacaggtgg ccgaagtaat tgaagcagtg
     4321 gctgcggagt tggcgtgtgt gaggttagat atgcggaaaa cattaccact ttctgttcct
     4381 acataaaggt gattggcatc ggcggaaagg ctcatgcacg aaacagtgcc tactacctta
     4441 gctacttgcc accattcagg ttcttttgta aaatcgagga tttctttggt ataataaact
     4501 ttatcgccca aagcgaggaa ggtttttgct tgtagaggat ctttaaaata aactacgtcg
     4561 cccttgtgta tatcgtgagg ggcaatggtt ttgaatggat aatcataaat ttggctacga
     4621 acaatgattt cttctccttc ggtaatgtct ttagatgcct taaaacgagt ggaatccatg
     4681 cttgtagggt cattgaacga ttcccagagt atcatggggc agtaagtatt tgccggggct
     4741 gacataccac tattgaggaa ggtagtagag aaactgaatc ctttatcttc tgtacggcgg
     4801 aatggcccac ctttaagaga taagataaat accttgggat tgatttttga aatggctaca
     4861 taaccgcctt ctccgctgtt gttaaagtca ccattccaca cttccactgc gcctatggga
     4921 gaattggtgc catcgcctat atattgtatc ccattcccca gagtacctcc aataggattt
     4981 ccgaaaccat tgacattgag tgtaacgaat tgtgaagtca tgtaggtgcg gttcaaactt
     5041 ttgaaataaa gttctccatc gatgttagta gaaatgccac catctgtgcc tataagaaag
     5101 gtttttggat cattgggttt aaaaacatag gtatgatgaa gctcatggag atataatgga
     5161 gagaatttac tcgtagcata acttgatata gcatctgtac cccaatagta atagccttca
     5221 ttgataaatt tgccatgcca catatttatg cctccaacta atatttcttc gggattgttg
     5281 gggtaaacaa cgatagtatt ggccaccaga ccgtaagcta cgttgttcac gacaaagata
     5341 tcgggtagca gagaagaatg gcctgggaaa ataatttccc atgtaatacc cttatcaatc
     5401 gaacgataaa tattgtcgaa atagccgtaa ggatcgctag cgcaagcata aatataatcg
     5461 ggattggaag gagccacagc taattctata cgtccaagcc ctatggaggg gagtttttct
     5521 ggatttaaaa cggtatcggg aagcacttgt tttgtacttt gattaataaa gccattaggt
     5581 tggcctaatt ccgaaacata aaccttactt tcgatcgaag ccactacaat tccgttgctt
     5641 cccaccttca cgtcccaaac gaaaccattc aagggctgca attgtccttc cccatctata
     5701 taatttgccg ttcgccattg ggattcatcg ctgttgcggt attttaaacc tgcattggtg
     5761 gccgcataaa tacgaccgtt ctgtatagct aattcgttga tataagccca ttcgagtaca
     5821 ttatctacca tggtaggcat tgttccagga accagactga aattgtcccc atcggtcgaa
     5881 acatataaac ccgtaccctg tgaaaaccca tattcaccag ttccagcata aatgtcgtta
     5941 ttatcggtct gagccataca acttacattc aggtttgagt tggcttgatt cacctttacc
     6001 caagtgcttc ccagattggt tgatttccat attcctcccg caacactggc agcataaacc
     6061 gtattaaaag tcgcatcacg cttatcgaaa agtatggcac gcgttcgccc acctacatta
     6121 tcaggcccca gactctgcca gttaagcgat actgcattgc ccgatttgag atgattttgc
     6181 aattgggctt tcccgggtcg gtccccttca tttcccttgc tcccagctat gcccgaagca
     6241 aataggtatg acccacatcc catcatcagg gccgtcagga aaaacagtaa agatttattt
     6301 ttcatatcat taagatttgt ttaaatatag ttcttatcct tttaaaatag tggcacaagt
     6361 tacatactct ttatgaatct gcaagtattt ctatgtaaaa aaatttccac aacattatgc
     6421 tcaaaacttt ttcggtgatc cttcataaag attatcaatc atttaattat ttcatcttac
     6481 aaaattaaat caatttttac tttcgaacat tcaacatgaa ataaaaattt attttctttt
     6541 catcattcat ttgtcgaatc atacttcatt tttgtaattg acaaattatc aaatgtatga
     6601 atataaaatc cattctttgt tttggaatac aaaagtaata attcctgaga tacttgtttt
     6661 aaagcaggtc cattttttta gatgtttcta tatccatacg cttctttgag tttgctgaca
     6721 gaatcagaaa tttttctttc ttcttcgatt gcctgcccgc aaaatagatt gcagcttttc
     6781 agttaacttt gcatcttcgg atttaatctt ttgggtttcc gccaatgcct ttggccataa
     6841 gcgaattcat ttattctaac aataatgaat ttcaattcga taaaagatga aagttttcga
     6901 agtttgttaa ggttgaagaa ttcatgaagg ggatttatcc atcgttcggg attatgcaat
     6961 gtcgacaaaa tctccgaatt taattttatg aactcaattt gaaatttatg agaacagaga
     7021 aggattttat gggggaactc cagttgccgg acgatgctct ttatgggata caatcgttga
     7081 gagctcgtaa gaattttccg catcattctc atttttttga agaatggtat cgggcgatgg
     7141 gcatagtgaa acacgcatgt tatctcactt atttatcgtt ttgcagagcg gtacagcaga
     7201 agaatttaga atcacgtttg ccttctcctc ttatatcttc tgaaataatg gatgggctcg
     7261 ttcaggcttc tattgaggtg agtcgaggtg agcatttcga acattttatc gttccgggaa
     7321 taagtggagg cgctggaacg agcatcaaca tgaatatcaa tgaaatcatc gccaacagag
     7381 cgcttatcat ttcgggccat tcaattgggg attaccacta tatcgatcct atactccatg
     7441 ccaatattta tcagtcgacc aacgataccg taccgactgc cttgaagata gccatgctga
     7501 atttgcttgg acagcttgag caagagatta atttattgcg agccgaaacg gaaagattgg
     7561 aaactgcttc gcgaaatatt ctaagagtag gacgcacaca aatgcaggat gcctcgcctt
     7621 cttcgtatgg aattttattt tctacatata gtgaggcgtt ttcacgcgat tggtggcgta
     7681 tgagcaaatg tttcgaacgc ataaaatcgg taaatctggg tggcagcgtt attggtactg
     7741 gcattggtgt gccaaggttt tttatcgtcg aagtcatcac tcatcttcag actttgacgg
     7801 gtttaccttt gagccgctcg gagaatatgc ccgatgcaac tgccaatctg gatagccttg
     7861 ccgaagttca tggaattata aaggctcatg caattaatct cgaaaaaatt gcaaatgata
     7921 tacggctgct ttcgtcggat atagcaagtc cgggcgagat cagcattcct cagaagcaaa
     7981 tcggaagcac aattatgcca ggtaaagcaa accctgtgat tcccgaatat gtgattagct
     8041 gcgttcatca tgtttatgcc aacgatgagg taattacttc tctggttgct tcgggctgcc
     8101 tcgactcgaa tgcttacctt cctatcattg gtcattgctt gatagatagc ctcaagacct
     8161 tgatctcggc taataaaaca ttgaaggata atttgttgaa cgatctgaaa tttcatccca
     8221 cagttgctgc tcataaaact tggaacagcc cggcgatcac tgtcgccttg agtccgttca
     8281 ttggatatta caaggcttca caattggctt gcgaaatgaa gaacacacat tgtgatgttt
     8341 ttacggctaa ccagcggctt catctgataa accccgataa actaaaggaa attatgcgta
     8401 cagaaaacct cttgaaactt ggatactccc tcaacgaatt ataattctta atatcatgat
     8461 caaaggaaaa gaagccagac ttcacattgg catttttggg cgaagaaaca atggcaaaag
     8521 tacattcatc aattgtttgt cggggcagga attatccata gtgagcgata tagccggtac
     8581 aaccaccgac cccgtaacca aagccatgga gattggagga ttaggtcccg tggtatttat
     8641 cgatacagcc ggcatcgacg acagcggacc cttagggcag aaacgcgtgc aaaaaaccct
     8701 tgaaatactc aaaaccattg acctggctat acttcttatt gccgaaaatg ccttcggtga
     8761 acaggaagaa tctattatac gccaattcaa agagtgggat gttcctttta tgattattca
     8821 tagcaaggaa gatctcatac ccatgaacaa acagactcta tcctgggcaa aaaatcagca
     8881 acccattgcc attgagactt tcagcccatc agattcgaac catttgcctg ctattattca
     8941 ggccatcagt aaatccatac ccgaatcggc ctataaaagc cgcagtttgg tgggtgattt
     9001 actcaattat ggagatgtag tgatgcttat tacacctatc gatatcgaag cccccgaggg
     9061 aaggcttatc ttgcctcagg tacaggtcat aagagatatt ctcgacaacg atgccatagc
     9121 cgtggtggta aaagaaagag aagccgatgc ctatattcga aaaatgtcgc ctcaaccagc
     9181 ccttgtcatc acagatagtc aggtgtttac gaaagctgat gcaattgtcc ccagaaatat
     9241 tcccctaacg agtttcagta ttcttctggc tcgacaacgg ggcgactttc aaaattattt
     9301 gaaaggaact cccaaaattt cacaattgaa ggatggtgag cgtgttcttt tactggaatc
     9361 atgcacacat caggtttcat gtgatgatat tggaagagtg aaaatcccgc gctggctcag
     9421 taattttaca ggcaaaaaga tagaatatga ggttgtatcg ggattggata gcctcccaag
     9481 acctatcacc gattatgccc taataattca atgcggaggt tgcatgatta cccgacggca
     9541 aatcatcaat cgcctgaaac ctgccgttga agccggaata cccatcacca actacggaat
     9601 ggctattgca tgggttcaag gaatttatcc acgagctata gcccccttta caggacaaac
     9661 agtgaattat tccgattatc tttgataaag aaaatcaatc ggctatgaaa ataccgacca
     9721 tatcattttt tacaatcgga atctttttac tgtttccccc tcctctctca ttttctcaat
     9781 ccgctactct gggccctgtt tctctttctc tgaaaaaagg aattgtgcta agatctttca
     9841 ccgataccaa aaatagagcc ttgactcctt caactttgat accttttttt gctttcgata
     9901 tagatgatca ggttgtcgat cctcaggagt ttaaatgttt gcaacaaggt gatagtcttt
     9961 attttagaac cgacaaaatg gaaggctcaa tggtttatat aaactacgac aagggagcaa
    10021 aattcaggct gaggattcgc aataaagata atgtaaaaca tagtgtttcg aatgttttac
    10081 cttttgggaa agattcacag agtatttata ttacgggtgc cgggaacttt gaatggcctt
    10141 attatctcaa tcgttcacag cttttcagac ctggatatgg acctataggg gtagttctac
    10201 cggataatgc ctggcatctt ggatttagcg atattccctt agaaatctcg aacaagcgtc
    10261 tttgcggctt ggcaagaaga gccggtataa gcaagggtaa ttttaccaga tggcacgccg
    10321 aactcgaacc aggaggggag gtgatctatc acctctggct cgacacccac gagggagaat
    10381 ggcaaaatgg cttgaaattg atgtttcagg aacgttatct gtacgatctg gatgacttta
    10441 ataattcgct ctatgagcgt catgatctat cttggataaa atctgcatgg attatactgc
    10501 ttcagtcggc ctgggatcac gaatggtatg atgcgttgat ccatcaatct atttatgaag
    10561 aaacactttc gaaatacgat cggatggtgg gtggctatga tgtttttgcg atatggccta
    10621 catggcctcg ccttggactc gaccagagat cacaattcga gctctatgaa gatcttcctg
    10681 gagctcgaat tgtg
//
LOCUS       DAOORU010000079        10653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7750_length_10653_cov_5.343084, whole genome shotgun sequence.
ACCESSION   DAOORU010000079 DAOORU010000000
VERSION     DAOORU010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10653
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7750_length_10653_cov_5.343084"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="PLF20_06215"
     CDS             <1..629
                     /locus_tag="PLF20_06215"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HOX80631.1"
                     /translation="LRLQFTPGTKFSYSNTGYAVLALLVERVSKTSFNEFLKNRIFIP
                     LGMYHTFTYDRQTMDTIATALGHRSSHPNTSLFEYDPIDEVLGDKSIFSTPADLFQYN
                     QAWTTDILIKENLRKEAFTRATLKNHSTVDYGFGWRFKKIEGKDIIYHNGLWHGFTAT
                     YTRIPDNELTIIILNNTNSHVSTIAQQIWKEIAPILELEQSQQSTAIN"
     gene            complement(1180..1335)
                     /locus_tag="PLF20_06220"
     CDS             complement(1180..1335)
                     /locus_tag="PLF20_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80632.1"
                     /translation="MSVPSDSLSWESKDKDSSPETWGNTSSPFGFLQVADKHALKRNL
                     VTCDKHL"
     gene            1516..4023
                     /locus_tag="PLF20_06225"
     CDS             1516..4023
                     /locus_tag="PLF20_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX80633.1"
                     /translation="MSFNKVLLISFSFIFIFTIENSFAQNTGQGGQSNPSNTKGNLPS
                     QKTGVIKGRIIDQSSKMPLEYANIAIFRKRDSALINGTITNEKGDFIIEKLPFGRFFV
                     KLSFIGYATRSIDSVFITPKQPEVNLGTIRIKLVENKLQGVEITEKRAPVEYNLDKKV
                     YNVDQNIVSMGGTAIDIMQTIPAVQVDLDGNVSLRGSSNVNILIDGRPSNLVSLDQLP
                     SSMVERVEVVTNPSARYDPDGTTGIINIILKKNRAEGINGMVSVNAGTKDKYNASINL
                     NYREKKFNFFTSYNFRQFSDEGTSFSHRETIINDTLSYLDQNGDSRRKGNFHNFTLGA
                     DYFIDNYNTLSLSGMYNIRGNDGRENMDNLSTNFKFDTLSLFNRTSINSNDNSGYQGI
                     LNYKRTFDDKTKELTADVYYSYSDGNSDNLITQKYTQLPNGLLGELIQQKTISDKSRN
                     MLTAQTDYVTSLWAGRLETGYKYRYSNEDSDYGFYNYDSADGWTSDFNRSNRFKYSES
                     IHAAYLIYTRDITDKLFIQGGLRAEAAQTTSDQVTQDSIFKNDYFSLFPTLHVRYNFN
                     NKNQIQLSYSRRVNRPWSRFLNPFIDYSDPLNLSAGNPALKPEYSNSLELGYTLQFGK
                     TTLTPSVFYTNTSGVISRIMSVDNNGVSFTTFKNQDKSSAAGLEVILAQQFAKWWRLN
                     TNFSYYHAEVDGPDITQQTASSNSWTFRLNSLMSVPRLADIQINFNYNSPMVFNPSIG
                     RHGGGGTQGKMEENYWADLGIKKNFLDGNASISLRLSDIFKTMKFNMTTYGSNFTSKS
                     ERTRDSRTLFVGLSYKFNDYKRRQQKLQDMIEELDEF"
     gene            4081..5559
                     /locus_tag="PLF20_06230"
     CDS             4081..5559
                     /locus_tag="PLF20_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOX80634.1"
                     /translation="MDYRKIIEQPIFQLIQKVASNQNIEVYIVGGFVRDLILGYESKD
                     IDILVVGNGIEFARSVADAMQPSPKVSVYKTFGTASLKYNDNWKLEFVGARKESYHKD
                     SRKPDVEPGTLEDDLKRRDFTINAMAISLNVNDFGTLIDPFHGENDLKNKIICTPLDP
                     LATFSDDPLRMMRAIRFATHLNFTIHFKVFASIKENAPRIQIVSIERTIDEFNKILMC
                     EKPSNGLNLLHRSGLLGQFFPEFVALEGIEYIDGKGHKDNFKHTLEVLDKLSQKSDNL
                     WLRWAALLHDIGKPATRRFDEETGWTFHGHETKGAKMVVQLFRKFKLPMNEKMEYVSK
                     LVMLHLRPIALVDKGVTDSAIRRLIFEAGDLIDDLMTLCEADVTSKNPEKVKKYLENF
                     RQVRKRINEVEERDRIRNFQPPITGDIIMEAFGIPPSHPVGVIKNAIKEAILEGTIHN
                     DYEEAFDYMVKEGNNLGLVTSGNIEEIRKRYITQQKLEDTSN"
     gene            complement(5636..6187)
                     /locus_tag="PLF20_06235"
     CDS             complement(5636..6187)
                     /locus_tag="PLF20_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOX80635.1"
                     /translation="MTEEIIIAGFGGQGVLSMGKILAYSGLMQGQEVSWMPSYGPEMR
                     GGTANVTVILSDERISSPVISQFDTAIILNQQSLDKFENSVKPGGLLLYDPNGISRHP
                     QRKDINIYKVEATTEAAKMGATKVFNMIVLGAYLKLKPIVKLENIEKGLTESLPARHH
                     KLIPLNLEAIKKGMEIVEIVHVV"
     gene            complement(6211..7008)
                     /locus_tag="PLF20_06240"
     CDS             complement(6211..7008)
                     /locus_tag="PLF20_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOX80636.1"
                     /translation="MTQNFDIPEIRTPENLVYKKTSVLTDNVLHYCPGCGHGTTHRLI
                     AEVIEEMGIQSEVIGVAPVGCSVMAYNYFDIDMQEAAHGRAPALATAIKRVFPEKFVF
                     TYQGDGDLAAIGTGETIHACNRGENITIIFINNAIYGMTGGQMAPTTLPDMPTSTCPY
                     GRDVKIMGNPLKITELLAMLPGTYYVTRQAVHSPALVRRAKKAIRMAFENQKLDRGVS
                     FIEIVSNCNSGWKLTPVQAIKWLEENMLPFYPLGDLKVNGELVKKSE"
     gene            complement(7013..8095)
                     /locus_tag="PLF20_06245"
     CDS             complement(7013..8095)
                     /locus_tag="PLF20_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOX80637.1"
                     /translation="MKELRLMKGNEAFAEAAIRAGADAYFGYPITPQSEVMEYLMAEK
                     PYERTGMIVLQSESELGAINMVYGAAACGKRALTSSSSPGISLMQEGISYIAGSELPC
                     LIMNVVRGGPGLGTIQPSQSDYFQATKGGGHGDYRLIVLAPSSVQEMFDFVSLGFELA
                     FKYRNPVMMLSDGVIGQMMEKVELSEQQGRLTNQQIDELYPWATTGKKPGKERRIITS
                     LELDPGRQEKHNLKLQEKYRKIETTEVRYETLHCDDAEYLLVAFGSSARICQKAMDLG
                     RSKGIKIGLMRPITLYPFPYSIIEQLSKQVKGILVVELNAGQMIEDVRLGSHDRIKVE
                     HYGRFGGMIPSPVDVLYAMEKQIIGG"
     gene            complement(8116..8337)
                     /locus_tag="PLF20_06250"
     CDS             complement(8116..8337)
                     /locus_tag="PLF20_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOX80638.1"
                     /translation="MAKVQGDVVIDIEQCKGCELCNVACPLNVLAISAEVNSKGYHYA
                     IKVNGNCNGCSNCAVVCPDGVITVYRAKV"
     gene            complement(8655..9926)
                     /locus_tag="PLF20_06255"
     CDS             complement(8655..9926)
                     /locus_tag="PLF20_06255"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOX80639.1"
                     /translation="MDKYLRELGLQFENWLISIGFSDFWAEKIADYIWLFSSLVLSVL
                     IYYLVRNIVYRIVKRIARRSKSLFDDFLLQRKFFQRLCYLVPAFIFKGFLPIVISEMT
                     GLIAFLDTLVSLYIVFVFLITIDAFLNAVHDLYLTTPISKNKPIKGFIQILKIIIYFI
                     GAVVVIAILLKRNPLAILGGLGAISAILLLIFRDSILGFVAGIQLSANNMVMIGDWIT
                     MQKYDVDGTVLEITLTTVKVENFDKTISFVPAYTLVSDSFRNWRGMEEAGGRRIARSL
                     QIDMGSVKFCTPEMLEQFAQIKLIRDYVLKKEEELRLFNQQFGVDGSVVVNGRRQTNL
                     GIFRAYIREYLKSNPDVHQGMTVMVRHLAPTEKGIPLQVYCFAKTTRWETYEKIQADI
                     FDHLIAVLPYFELKVFQNPTGSDFRALVGQS"
     gene            complement(10266..10610)
                     /locus_tag="PLF20_06260"
     CDS             complement(10266..10610)
                     /locus_tag="PLF20_06260"
                     /inference="COORDINATES: protein motif:HMM:NF024276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HOX80640.1"
                     /translation="MNKIFCWFFIFLLMAGCYRSNNPQKVAEIFLHDISTGNFRHARK
                     YVTPNTLSYLINAESILPLHDSIKSHPVFYKIEKIEMNGDTARCDIIWDETKENLLII
                     RQQGRWLVDLNI"
BASE COUNT         3388 a         2295 c         1963 g         3007 t
ORIGIN      
        1 aacttcgctt gcaatttacc cccggcacca aattcagtta ttccaataca ggttatgccg
       61 ttttggcttt attggtcgaa agggttagca aaacttcttt caatgaattt ctgaagaata
      121 gaatatttat ccctttaggc atgtatcata ctttcaccta cgatcgccaa acaatggata
      181 caattgctac agcattggga catcgttcgt ctcatcccaa tacttctctc ttcgaatatg
      241 atcctattga tgaagtttta ggcgacaaaa gtattttctc tacaccggcc gatctatttc
      301 agtataacca ggcatggaca actgatattc tcattaaaga aaatctcaga aaagaagcct
      361 ttacacgtgc taccctcaaa aatcatagta cggtggatta tggctttgga tggagattca
      421 aaaaaatcga gggaaaagac atcatctatc ataacgggct atggcatggg tttactgcaa
      481 cctatacccg gatacccgac aatgagctta ctatcatcat ccttaataat actaattcgc
      541 atgtatcgac tattgcgcag caaatatgga aggaaattgc tccaatactc gaactcgaac
      601 aatctcagca aagcacggct atcaattaag tattgcgtcg tctacgatct aatgagctct
      661 tcacttccct ggaatcttcg gaaaatttca tacccctttc tggatatcaa tcgcaacttg
      721 ccttctattt tgcatcccat cgaacctgca ttcaaaccga atcacacgga atacaaaata
      781 tgattccgaa cccaatcgat tttgccgaaa tagccggcaa tcgtattcat catcaggtat
      841 gtgaagcccc tcgctatctc agatgtagat tgatcttgct gcatgcaact gctaaagcag
      901 cagataaggc ttcaaaatgg ataatctcct gaaagaaatc actaatgaaa accccaaggt
      961 tattctctca caaataataa cttcgtttgt tatcccagtc cgattatgtt gatcgtaaaa
     1021 gcaatcgttt taccaatgca ggagtagtca aacaatctaa aacacaatta aatataaata
     1081 gataaacatg gtgaagtcgg atttttgagc agaagacaac ttgtaatatc cccgaaggtt
     1141 actactaaag ctcgagtaat gttagttcac aaaaagatat tacaaatgct tatcacaagt
     1201 cacaagattt cgtttcaagg catgtttatc ggccacctgt aaaaaaccaa atggactgga
     1261 tgtattgccc caggtttcag ggctactatc tttatccttc gattcccagg ataaagaatc
     1321 agaaggaacc gacatgaaga caaattcatc gataatataa agccaattgt acttcgttct
     1381 ctgaacaaga atttttgaag aaaacgattt gccttatgtc atgaatcagc aatagtgtag
     1441 taaaattatt actttctcta ttaaaccaat taattctttt tccctcaaat agaggaaatt
     1501 aaaatattga aatttatgtc attcaacaaa gtattattaa tcagcttttc attcattttt
     1561 atttttacga tcgaaaattc ttttgcacaa aacacagggc aaggagggca aagtaatcca
     1621 agtaatacga aagggaatct tcccagtcag aagacagggg tcattaaagg acgtatcata
     1681 gatcaaagct cgaagatgcc actcgaatat gctaacattg ccatatttcg caaacgcgat
     1741 tcagcgctta tcaatggaac cattactaac gaaaagggcg attttatcat cgaaaaactc
     1801 ccattcggcc gtttcttcgt caaactttca ttcataggat atgctactcg tagcattgat
     1861 tctgtattta ttactccaaa gcaacccgaa gtcaatttag gaactataag aatcaagctg
     1921 gtcgaaaata agcttcaagg ggttgagatc acagaaaaaa gagcacccgt agaatacaac
     1981 ctcgataaaa aggtttacaa tgtcgatcag aacattgtaa gtatgggagg aacagccata
     2041 gatatcatgc agactattcc tgcggtgcag gtcgatctcg acggaaatgt aagcctgagg
     2101 gggtcgagta acgtcaatat cttaatcgac ggcagaccga gcaacttggt cagcctcgac
     2161 caattaccct caagtatggt cgaacgggtc gaagttgtta ccaacccttc ggctcgttat
     2221 gaccccgatg gaactactgg catcatcaat atcattctga aaaagaaccg cgctgaggga
     2281 attaacggta tggtaagcgt aaatgcagga acaaaggata aatacaatgc ctccattaat
     2341 ctcaactacc gtgagaagaa attcaacttc tttactagtt ataatttcag gcaattttcc
     2401 gacgaaggaa ccagtttttc acatcgagaa accattatca acgacacact ttcctattta
     2461 gaccagaatg gagattcacg acgcaaagga aatttccaca attttacttt gggggctgat
     2521 tattttatcg ataactacaa tactttatca ctaagtggta tgtataatat aagaggaaac
     2581 gatggaaggg aaaatatgga taacttaagc accaacttta aatttgatac cctaagcctc
     2641 tttaaccgca cctccatcaa ttccaacgac aattcaggct accagggaat cctcaattac
     2701 aagcgaacat tcgacgacaa gacaaaggaa ctcacagccg atgtatatta ttcctattcc
     2761 gacggaaatt ccgataatct aatcacacaa aaatatactc agcttccaaa cggattgcta
     2821 ggcgaattga ttcagcaaaa aaccatttcg gataagagcc ggaatatgct tacggcacaa
     2881 actgattatg taaccagttt atgggctggg cgcttggaaa caggatacaa ataccgttat
     2941 tcgaacgaag attccgatta tggtttttat aattatgatt cggctgatgg gtggacaagc
     3001 gattttaacc gaagtaatcg tttcaaatat tccgaatcta ttcacgcagc ctatcttatc
     3061 tatacccgcg atattaccga taaacttttc atccagggag gacttagagc cgaagccgct
     3121 cagactacaa gcgatcaggt aactcaagac tcgatattca agaatgatta tttcagcctc
     3181 ttccctacct tgcatgtccg ttataatttt aataacaaga atcaaattca actcagctac
     3241 tcacgtcgtg taaacagacc atggagccgt ttcctcaatc cctttatcga ctattccgac
     3301 cccttgaacc tttcagcggg taatcctgca ctgaaaccag aatacagcaa cagtctggaa
     3361 cttggatata ccttacagtt cgggaaaacc acactcactc cttcggtttt ctacaccaat
     3421 accagcggag tcatttcacg tatcatgtcg gtcgacaaca atggagtctc ttttactacc
     3481 tttaaaaacc aggataaatc ctctgcagct ggacttgaag tcattcttgc ccagcaattt
     3541 gcaaaatggt ggagattgaa taccaacttc agttattatc atgccgaagt agatggcccg
     3601 gatataaccc agcaaacagc ttcgagtaat tcatggacat tcaggcttaa ttcgttgatg
     3661 agtgttccgc gactggccga tattcaaatc aacttcaatt ataattctcc tatggttttc
     3721 aatccttcca ttggcaggca cggcggtggt ggcactcaag gaaaaatgga agaaaactac
     3781 tgggctgatt taggaataaa aaagaacttc ttagatggaa atgcaagcat ttcgctccga
     3841 ttgagcgaca tattcaaaac catgaaattc aatatgacta cttacggcag caactttacc
     3901 tccaaaagtg aacgtacacg cgatagtcga accttgttcg tgggtctttc gtacaagttc
     3961 aacgattaca aacgccgcca gcaaaaattg caagatatga tagaagaact ggatgaattc
     4021 taacagctac tgttctttct tgggccgaaa agggtaaatt tgcacccttt ataaataatt
     4081 atggattatc gcaaaataat cgaacaaccc atttttcagc tcatacaaaa agttgcgagt
     4141 aatcagaata tcgaggttta tattgtgggg ggatttgttc gcgacttaat actgggatat
     4201 gagtcgaagg acatagatat tcttgtggta ggcaatggaa tcgagtttgc tcgttcagtg
     4261 gcagatgcca tgcagccatc tccaaaagtc tcggtttata aaacttttgg aaccgcaagt
     4321 ttaaaatata acgataactg gaaattagaa tttgtaggag ccagaaaaga atcctatcat
     4381 aaagattcac gtaaacccga cgtagagccc ggtacactgg aggacgattt aaaacgtcgt
     4441 gattttacca tcaatgctat ggcaataagt ctgaatgtca atgactttgg aactttgatc
     4501 gatccctttc atggagaaaa cgacttgaaa aataaaatta tatgcacacc cctcgatcct
     4561 ttggctactt tttcggatga tccactcagg atgatgcgtg ccattcgatt tgctacacat
     4621 ctgaatttta ccattcactt caaagtcttc gcttccatca aagaaaatgc tcctcgaatc
     4681 cagatagttt ccatcgaacg cactatagat gaattcaata aaatactaat gtgcgagaag
     4741 ccttccaatg gtttgaatct gttacacagg tcaggattgc ttggtcaatt tttccccgaa
     4801 ttcgtagccc tcgaaggcat agaatacatc gacggaaagg ggcataaaga taacttcaaa
     4861 catacattgg aagtcctcga taaactttcc caaaaatcag ataacctgtg gctgcgatgg
     4921 gctgctttgc ttcacgacat cggtaaaccc gctactcgac gtttcgacga agaaaccggc
     4981 tggacctttc atggacacga aaccaaagga gccaaaatgg tggttcagct cttcagaaaa
     5041 ttcaaactcc ctatgaacga aaaaatggaa tatgtttcga aactggtgat gcttcatctg
     5101 cgccccatcg ctctcgttga caagggagta accgattcgg ccatacgccg cctgattttc
     5161 gaagccggag acctgatcga cgatctgatg acactttgcg aagccgatgt tacttctaaa
     5221 aatccggaaa aagtaaaaaa atatctcgaa aattttcgac aggtacgcaa aagaataaat
     5281 gaagtggaag aaagagaccg aataagaaat tttcaacctc ctatcactgg tgatatcatt
     5341 atggaagcct tcggaatacc tccctcccat cctgtgggtg ttataaaaaa cgccatcaag
     5401 gaagctatcc tcgaaggaac gattcataac gattatgaag aagccttcga ttatatggtg
     5461 aaagaaggca ataatttagg tctggttact tcgggcaata tagaagaaat aagaaaaaga
     5521 tacataactc aacaaaaatt ggaagatacc tccaattaaa tttgacaaag gatataaaaa
     5581 aaagccctgt taagtaatta acagggtttt gatgattcga cttttgattc gaactttaaa
     5641 caacatgaac gatttcgaca atttccatgc cttttttaat tgcttcaagg ttaaggggaa
     5701 tcagtttatg atgacgggct gggagagatt cagtaaggcc tttttctata ttttcgagct
     5761 tgacgatggg tttaagcttc agataggctc ccagcacgat catgttaaaa acttttgtgg
     5821 cacccatttt tgctgcctcc gtagttgctt cgactttgta gatattgata tcttttcgtt
     5881 gagggtggcg agaaatgcca ttagggtcgt agagcaaaag gccgccgggt ttaacactgt
     5941 tttcgaattt atcgagcgat tgttgattca gaatgatggc agtatcgaat tgactaatta
     6001 caggactgct aattctctca tcgctcagaa taaccgtaac attagctgtt ccaccccgca
     6061 tttcagggcc ataagaaggc atccagctaa cttcctggcc ttgcataagt cccgaataag
     6121 ccagaatctt gcccatcgaa aggactccct gccccccaaa tccggctatg ataatttctt
     6181 cagtcataag taattaaagg tattagatta ttattctgat tttttaacca attcgccgtt
     6241 tacttttaaa tcgccaagtg gataaaacgg caacatattt tcctcgagcc atttgatggc
     6301 ctgcacaggg gttaatttcc agcccgaatt gcaattggat acaatttcga tgaagctaac
     6361 tccccgatcg agcttctgat tttcgaaagc cattcggatg gcctttttgg ctcttctgac
     6421 cagagctgga gaatgcacag cctggcgagt aacatagtac gttccaggaa gcatggctaa
     6481 taattcggta atttttagcg gatttcccat tatttttaca tcgcggccat aaggacaagt
     6541 actggttggc atatcgggaa gtgtagtagg agccatctgc ccgcccgtca taccataaat
     6601 ggcattattg ataaaaatga tggtaatatt ttcgccacgg ttgcatgcat gaatggtttc
     6661 accagtacca atagccgcaa gatcgccatc gccctgataa gtaaatacaa atttttcggg
     6721 aaatacacgt ttgatggctg tggcaagtgc tggcgcccta ccatgcgctg cttcctgcat
     6781 atcgatatcg aaataattgt aagccattac ggaacacccc acaggtgcca cgccgatgac
     6841 ttcgctttgt attcccattt cttcgatcac ttcggctatc aatctgtgcg tagttccatg
     6901 accacatccc ggacagtaat gaaggacatt atccgtaagc acactggttt ttttataaac
     6961 taaattttcc ggggtacgaa tttcgggtat atcaaaattt tgtgtcattt ttttaacctc
     7021 caataatttg tttttccatt gcatataata catcgaccgg agagggaatc attccgccaa
     7081 aacggccata atgttcaacc tttattctgt cgtgcgatcc cagacgaaca tcttcgatca
     7141 tctggccggc attgagctcg acaaccagaa ttcctttcac ctgtttcgaa agttgttcta
     7201 ttattgaata ggggaaagga taaagagtaa tgggtcgcat aagtccaatt ttgattcctt
     7261 tcgaacggcc caaatccata gctttctggc aaatacgagc cgacgaacca aaggcaacca
     7321 gtagatattc ggcgtcatcg caatgcagcg tttcatacct gacctctgtt gtttcaattt
     7381 tgcggtattt ttcttgcagc tttagattgt gtttttcctg cctgccaggg tctaattcga
     7441 gagaagtgat gatgcggcgt tccttgccgg gtttcttccc ggtggtagcc catggataaa
     7501 gttcgtcgat ctgctggttg gtgagccttc cctgctgttc gctcagctcc actttttcca
     7561 tcatctgccc aataactccg tctgagagca tcatcacagg atttcggtat ttgaaagcca
     7621 gttcaaaacc taagctaaca aaatcgaaca tttcctgaac cgatgaagga gcaaggacta
     7681 taagcctgta atcgccatgt ccgcctcctt tggtagcttg aaaataatca ctttgcgagg
     7741 gctgtatggt tcccaagcca ggaccacctc ttactacatt catgatgagg caaggcaatt
     7801 cagaccccgc aatataggat ataccttcct gcataagact gataccaggg ctcgaggaag
     7861 aggttaaagc acgcttgccg caggcagcag ctccataaac catgttaatc gccccaagct
     7921 cactttccga ttgaagaaca atcatacccg tgcgctcata aggtttttcc gccattaaat
     7981 actccataac ttcgctctgc ggtgtaattg gataaccaaa ataggcatcc gctcccgctc
     8041 tgatggcagc ctcagcaaat gcttcgttgc ctttcattaa ccttaattct ttcatgcctt
     8101 ttcttttaaa agttgttaaa ccttagctct ataaacggtg attacaccat caggacacac
     8161 aactgcacaa tttgaacaac cattgcaatt cccgtttacc ttgattgcat aatgatagcc
     8221 tttgctattc acctcggccg atattgccag cacatttaaa gggcaggcaa cattacaaag
     8281 ttcacagcct ttacattgtt caatatcaat gactacatct ccttgtactt ttgccatata
     8341 attaattttt agtttaaaat aataatcgcc atttgaattg acgaaattac atattataat
     8401 tgagataccg aaaacgtttg caagaacttc gcctcgactt tctactaatt taaaataatt
     8461 ctaaatgaaa aatactcatt caaaaaattc atttatagtt tattgtaacg atacaagtga
     8521 gcaatataaa tattttattt tctttctcat ccatcattcc taaataataa ggccaataga
     8581 aaaacaaaac gcaaattccg ttcaaaaaaa tcaatgaaat ttagccagca aataaaccat
     8641 aaaaatggac atccttacga ttggcccacc aaagccctaa aatcgcttcc ggttggattc
     8701 tggaatacct tcaattcgaa atagggtaaa acggcaatca aatgatcgaa aatatcggcc
     8761 tgaatctttt cataagtttc ccaacgggta gttttggcaa aacaataaac ctgcaagggg
     8821 atcccttttt cagtaggagc cagatgtctt accataactg tcattccctg atgtacatcg
     8881 ggattgcttt tcagatattc acggatgtaa gctctgaaaa ttccaagatt ggtctgccgg
     8941 cggccattaa ctaccacaga accatcaaca ccaaattgct ggttaaatag tctaagttct
     9001 tcttccttct tcaacacata atcccttatt agtttgattt gcgcaaattg ttctaacatt
     9061 tcgggagtac aaaattttac acttcccata tcaatttgaa gagaacgagc aatacgcctt
     9121 cccccggctt cttccatgcc tctccagttg cggaatgagt cagaaaccaa tgtatatgca
     9181 ggtacaaacg aaatcgtctt gtcaaaattc tctactttta ctgtcgttag cgtaatttcc
     9241 aaaacagttc catccacatc atacttttgc atggttatcc aatcgccaat catgaccata
     9301 ttgttggccg atagctgaat tccagcaacg aaaccaagta tgctatccct aaaaatcaat
     9361 aacaaaatag ccgaaatggc acccagcccg ccaagaattg ccagaggatt tcgcttgagc
     9421 agaatggcga taaccaccac tgcacctata aaataaatga tgattttcag aatctggata
     9481 aaccccttga tgggtttgtt tttcgatatc ggtgtagtta aatataaatc gtggacagca
     9541 tttaaaaaag catctatagt gataagaaag acaaaaacaa tgtaaagaga tacaagagta
     9601 tcaagaaagg caataagacc cgtcatttca cttatgacga taggaagaaa tcccttgaaa
     9661 ataaaggccg gtactaaata gcaaagcctc tggaaaaatt tacgctgcaa aagaaaatcg
     9721 tcgaacaggc ttttcgatcg tctggctatg cgtttgacta tcctgtaaac gatgttgcgt
     9781 accagatagt aaataagaac agaaagaacc aaagacgaaa aaagccaaat ataatcagca
     9841 atcttttcgg cccaaaaatc agaaaaacct atcgaaatca gccaattttc aaattgcaaa
     9901 cccaattctc ttaaatattt atccatcgat ctgtgtttat ggggtcaata attagcaata
     9961 actcatcatg aaaatcattc aataaaacct gattaaataa tctcgagctt ccatgaattt
    10021 acaaaaatac aaacatcttt attcgaacga atgaacattg tcgaaatatc gaaacacaga
    10081 attccgaatc atcggtcaga aatttccgaa taagcctctc ttatctactt ctttaaaccg
    10141 taaaaaaata tttcctactc cataatttgg aataacagga gaaataggct ctcctcgaaa
    10201 ccatcaaaat tctattcatt ctctcgaatt gccaagaaaa tctcttctcg attgcatggc
    10261 agttattaga tatttaaatc caccaaccat cggccttgtt gtcgaataat gagtaaattt
    10321 tctttggttt catcccagat tatgtcacat cttgcggtat ctccattcat ttcaattttt
    10381 tcgattttat agaaaacagg atgagatttg atagaatcat gcagtggtag tattgattcg
    10441 gcattaatca ggtagctgag ggtatttggg gtaacatatt tacgagcatg acgaaaatta
    10501 cccgtactta tatcgtggag aaaaatctcg gccactttct gaggattgtt ggagcgataa
    10561 caacccgcca tcaagagaaa aataaaaaac cagcaaaata ttttattcat aatgaaatat
    10621 tttataaggc attgaattgt ttatttttac acc
//
LOCUS       DAOORU010000080        10408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7978_length_10408_cov_5.153675, whole genome shotgun sequence.
ACCESSION   DAOORU010000080 DAOORU010000000
VERSION     DAOORU010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7978_length_10408_cov_5.153675"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="PLF20_06265"
     CDS             complement(<1..1068)
                     /locus_tag="PLF20_06265"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOX80641.1"
                     /translation="MKTQISYLFTLIMLAFSWQAFAQTVIQGRICDFDTHQPLSGTDI
                     TVESTGEGTVSNSKGEFTLITKMEKGRLLISHIGFQNETINFDASQGNIIKLNEISLR
                     RLVINLSPVNILAFTVREQTDPVTVNSINRKMIHQISGDHSYPEIMRMMPGVYSSRTG
                     GGVGDAAINIRGFKQENLALTLNGIPIGSVENGLVYWSNWDGLQEATENIQVQKGIGA
                     SVLVNNSLGGTINIITNADPSLTGGMLSTSITDYGLQRTSFKYSSGINPNGLSISFMG
                     SHTTGQGYVDGTQVNGFAYYLSISKQLNPSHLFTFNAIGSPERHGQRSTLLSYAEIEE
                     RGIKYNKDWGTYGGIRKNLAEN"
     regulatory      complement(1156..1274)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(1319..3307)
                     /locus_tag="PLF20_06270"
     CDS             complement(1319..3307)
                     /locus_tag="PLF20_06270"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOX80642.1"
                     /translation="MKTKLTIILSLVLGLGILNSGKAQQVFERGSYRCHLDKIKKYST
                     EYFRNEEKNYFPKSFDVLKYQLDVDIWSCYQSPYSHAFDGKLVITLKADSLLDHVKLN
                     AVNSSLQIQEVSIAASTFTHSGNILTINLDTTMIPGDTFQIAIKYHHNNVNDYAFYAK
                     NGFVFTDCEPEGARCWFPCYDRTSDKAQWELTAKVPSNVKLGSNGILQDSVKIADTIY
                     YTWKSDENVATYLIALTSRTNYNLKIINYTNPNDTSEHFPFRFYYNQGENPQNIINVI
                     DDVTTYFSSTFCKHPFPKNGFASLNDEFQWGGMENQTLTSICPNCWDESLIVHEFSHQ
                     WFGDMITCATWADIWLNEGFATFSESLWLGEKSGYNAYKQDVLQNASYYLSANPHWPI
                     SDPSWAITTPSSDILFNYAITYCKGACVLHQLRYTLGDSIFFNIIHQYAHDSSFRYQS
                     ATIPDFINIVNSVTGEDYQWFFDEWIYMPNHPIYQNYYSITQIDDQHWGLYLEVNQTQ
                     TNTGFFKMPITIRIVGLNELDTLIRVMNDVNHQGFSFIFDKKPIGVYFDPNSDIVLKQ
                     ASIAVGIAQPLSERFTVSEIYPNPVQHELYMNISVNKASLSEINIFNVSGTKVYTNMY
                     NLLPGFQTLRLNISSLPSGIYFIEINWEGKKVQRKFVH"
     gene            complement(3379..4125)
                     /locus_tag="PLF20_06275"
     CDS             complement(3379..4125)
                     /locus_tag="PLF20_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80643.1"
                     /translation="MPNEPSEFSDAEEFYHYITEHSSNIRIVDDIINVKDQEQFLRLA
                     RKLKPNKVGDEHALKMAARLFESDLTIAEGKKILVILALRSNIEAFRVIEKYKEVAPP
                     KLQDFARFAYYISRTLIEGELSDNPPSLISTGLGGKDNKLRMFIAIFPSFEGTFSENQ
                     ARIIRMEWEETCKNYQIETEKLRFLFEYTSLRILVPLNIDLSAVVKEFIAECNKYGNF
                     VYEGFLATNVAIPSHREILSAIERIKNHPY"
     gene            complement(4141..5022)
                     /locus_tag="PLF20_06280"
     CDS             complement(4141..5022)
                     /locus_tag="PLF20_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HOX80644.1"
                     /translation="MKKIYFIINPAAGIHHSRKQKLEEMIPEYFPAETFQTEIVRINE
                     KDRISDLTQKAVAKGYDIIVAVGGDGTINEIAQVLVDSSAVLGIIPAGSGNGLAHFLK
                     IPFKLEEALNLIKNGEAMCIDTIQLNDRIAVSIAGMGFDARIARQFALDNHRGFFTYF
                     KIAVKEYITFRPRLFQVIANNFIIKERAILLSIANSNQFGYNTFIAPKASITDGLADI
                     TIVKKIPYSKILRTVVLLFTHKIDKSLYVNTYKADQFTVIRETGKYVNIDGEPFKMDK
                     EVHVKVKPHSLRILIPL"
     gene            complement(5019..6965)
                     /locus_tag="PLF20_06285"
     CDS             complement(5019..6965)
                     /locus_tag="PLF20_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HOX80645.1"
                     /translation="MKIKFVWKNGTHLIVAYRLLIALIVFSLSRLCLYWFQHDLFPSE
                     AIPSIFTIWRLGLRFDLSAVIMLNLPYIFLATLPFPFRNHKYYRNFSSFLFYFANAIG
                     ILLNIIDSAYYPFTLKRLTWDVTGFLKATNNMGLLLPKFLSDFWIYLLVFFVLFSIFV
                     LLTTRLRYEKKLFYQSKWNFFIWNLFLMIVWGGIAIVGIRGGFQRRPIYLVNAAEMIS
                     PDYDALILNTPFTIIKTYGKPSLKEVVYYEDEAEAASIFQPLKNYYRATQPTTKFNVV
                     IIIMESFSLEHSALMNPQLYKDSTEGYTPVLDSLMRHGLTFRAYANGKRSIEAIPAIC
                     SSLPSLMPVEFINSPYVSEPISSLPLSLKPLGYHSIFYHGGMNGTMHFDAFSRLSGFD
                     EYKGKNEYRGPEAFDGRWGIFDEPFFQFMAKDLSNQTQPFLVVFFSLSSHHPYVVPAK
                     YMNRFGPGKLKIHQSIRYADYALGQFFKMASQESWFKNTLFIITADHTSEAFSPYYLT
                     PVGQHVIPLIFYLPDNSFRGFSSQIAQQCDIYPSVMDFLNAEGSFVAFGRSVFDITST
                     PFGISYLNQEYQLIYKNKCLRYFNEKVSGFYDLKNDSLQTNNLAAEQLPEQDEALRFL
                     KAIIQQYNNRIIYHRLRADQMVKP"
     gene            complement(6989..7660)
                     /locus_tag="PLF20_06290"
     CDS             complement(6989..7660)
                     /locus_tag="PLF20_06290"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HOX80646.1"
                     /translation="MKRFLFFCICACLFTSFTLTGCIDEVKDDTPPFITVVGSNPVYI
                     RLNSPFYDSGVIITDNYGVFKMWIDTSELNIHKAGRYKVYYFAEDFNNNVANAERTVI
                     VRIEGANLTGKWSGERISPYPGGISVLFTDSLYNPGTRHIYFEQIGGLYPSLIKADLL
                     GYLGDTLGFSKQIIEITDTSTTYFLASGTIQHDGKSFQLFYHLITETATSLDTSYISQ
                     LIYHK"
     gene            7821..8909
                     /gene="ychF"
                     /locus_tag="PLF20_06295"
     CDS             7821..8909
                     /gene="ychF"
                     /locus_tag="PLF20_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOX80647.1"
                     /translation="MGLQCGIIGLAGVGKTTLFNCLSSSRARAGGGGSKSNLGMIYVP
                     DDRLQKIDALIKSARIVPTTVDIVDIPGLVRGAGKGEGVGSKFLADIRNTDALIHVLR
                     CFDEPSVPHVESSIDPVRDIEMVELELIVRDLESIEKKLQRLEKQAKSGDREARTGIE
                     VLEVYKHHLEDFLPARTAPVGKEFQRYLTDLFLLSAKPVMYVCNVDDDSAITGNAYSN
                     AVRDFFDDQNVEILTIAGKLESEIAELDDPADRRAFLEEVGLNEPGVNKLIQSAYRLL
                     NLQTFFTAGPKEVRAWTIRKGITAPEAAGVIHSDLERGFIRAEVMKYDDFIRLGSEHA
                     CREAGKLFIEGKNYVVQDGDILHIRFNV"
     gene            complement(8983..9363)
                     /locus_tag="PLF20_06300"
     CDS             complement(8983..9363)
                     /locus_tag="PLF20_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80648.1"
                     /translation="MYEHKNHKLSKYLKVAGLITGIVGLLFFLVFYLIKRVTFTEGTK
                     LPHIIDLMLLVGMAFIGCIIGFWHTGEAGIILIFSAVIFFLQFMGGHLSLSFGGFVYA
                     LPFILSGGLFLSSYFVANRLKNKK"
     gene            10055..10279
                     /locus_tag="PLF20_06305"
     CDS             10055..10279
                     /locus_tag="PLF20_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80649.1"
                     /translation="MQKCITSVLQEMESALEMCGIRITSYVSNISSKSIQKIIDSIVD
                     GSDDMDQLEELAHSRIGNKHRERKEEMELL"
BASE COUNT         3199 a         1921 c         2236 g         3052 t
ORIGIN      
        1 gttctcggca agattcttgc gtattcctcc ataagttccc caatccttat tgtacttgat
       61 tcctctttct tcgatttcgg catacgataa caaagtgctt ctttgtccat gtcgttcagg
      121 tgaaccaata gcattgaagg tgaaaaggtg agatggattc aattgtttgc tgatagataa
      181 ataataagca aatccattta cttgagttcc atctacatag ccttgtcctg tggtatgcga
      241 tcccataaag ctgatgctta atccattggg gttgattccg ctagaatatt taaaagaagt
      301 acgttgcagt ccataatccg ttattgaagt gcttaacatt cctccagtta atgaaggatc
      361 tgcattggta ataatattga tagtgcctcc tagtgaatta ttcaccaaaa ctgaggctcc
      421 tatccctttc tgaacttgaa tattttcagt ggcctcttgc agcccgtccc aattcgacca
      481 atataccagt ccattttcaa cgcttccaat aggaattcca ttcagggtca aagcaaggtt
      541 ttcttgtttg aaacctctga tattgatggc tgcatcacct accccaccgc ctgtacggct
      601 cgagtaaacc ccaggcatca tccgcatgat ttctggatag gaatgatccc ctgaaatttg
      661 atgaatcatt tttcgattga tggaatttac ggttacggga tcggtttgtt cgcgtacagt
      721 aaaagccagg atatttacag gtgaaaggtt gattaccaat cgccttaaag atatttcgtt
      781 caatttaatg atatttcctt ggcttgcatc gaaattgatc gtttcgtttt ggaatccaat
      841 atggctgatg agcaaacgcc ctttttccat tttggtgatc agcgtaaact caccctttga
      901 gttgcttact gtgccttctc cagtcgattc cacagtgatg tcggtacccg ataagggttg
      961 atgcgtgtcg aagtcgcaaa ttctaccttg aataaccgtt tgagcaaatg cctgccaact
     1021 gaatgcaagc atgatcagag tgaataaata agaaatttgt gttttcatat tgcttgataa
     1081 taaaaatgcc ctgatttgtt tcagggcaga atgatcttta ccgagcaatg gagcaccaaa
     1141 agaatagtgc gtttgacatt tcttcttcca tccagacttt gactgtcggt accggagttt
     1201 caccggttca gtccctttgg ggagttgcgg actttaccgc cgatcgggaa tttcaccctg
     1261 ccctgaagaa aatcgctgca aaaataaaga aattttattg ccaacaatgt gatttgtttt
     1321 aatgcacaaa ttttcgttga acttttttgc cctcccaatt tatttcgatg aaatatatgc
     1381 ctgatggtag gcttgaaata ttaagtctta aagtttgaaa accgggtaat aaattgtaca
     1441 tattggtata aaccttagtg ccagaaacat taaagatatt gatttcagac agagaggctt
     1501 tattaacgga aatattcatg tataattcat gctggacagg attaggatag atttccgaaa
     1561 ccgtaaatct ctcagagagg ggttgggcaa taccaacagc aattgaggct tgtttaagca
     1621 caatatcact gttgggatca aagtaaacgc cgatgggttt tttatcgaaa ataaacgaga
     1681 atccctggtg atttacatca ttcattactc tgatcagagt atcgagttca tttagtccca
     1741 caatccttat agtgattggc attttaaaga atcccgtatt ggtttgagtt tgattaactt
     1801 ctaaatataa tccccaatgt tgatcgtcaa tttgcgtgat gctgtaataa ttttggtaaa
     1861 taggatgatt gggcatatat atccattcat cgaaaaacca ttgataatct tcacccgtaa
     1921 ctgagtttac aatattgatg aagtcaggga tagtagctga ttggtatcga aacgatgaat
     1981 catgggcata ttggtgaata atattgaaaa atattgaatc gcccaaagta tatcttagct
     2041 gatgaagtac acaagcgcct ttacagtaag ttatggcata gttgaacaat atatcagagg
     2101 agggagtagt aatagcccaa cttggatcag aaataggcca atgtgggtta gctgatagat
     2161 aataggaagc attttgaagc acatcctgtt tgtaagcatt ataacccgat ttttctccca
     2221 gccagagact ttcgctaaag gtggcaaatc cttcgttaag ccagatatcg gcccaggttg
     2281 cacaagttat catatcgcca aaccactgat gtgaaaattc atgtacaatg aggctttcgt
     2341 cccagcaatt gggacaaatg ctcgtaagag tctggttctc cattccaccc cattgaaatt
     2401 cgtcgttcag gcttgcaaat ccattcttgg gaaagggatg cttgcaaaaa gttgaactaa
     2461 aataagttgt tacatcatct ataacattga tgatattttg aggattttcg ccttgattgt
     2521 aatagaacct gaaggggaaa tgctcggatg tgtcgttagg attcgtataa tttatgatct
     2581 tgaggttgta attggttctc gaagtgaggg caatgagata cgtagccacg ttttcatcac
     2641 ttttccaagt ataatagata gtatcagcaa ttttgaccga atcttgcaat ataccgttac
     2701 ttccaagttt tacattggaa ggtactttag cggtcaattc ccattgggcc ttatcagatg
     2761 tccggtcgta acagggaaac cagcagcggg caccttcggg ttcacaatcg gtaaatacaa
     2821 agccattctt agcatagaag gcgtagtcat ttacattgtt atgatgatat tttatggcaa
     2881 tctgaaaggt gtcgcccggt atcatagtag tatcgagatt gatggtaagg atgttaccgc
     2941 tgtgggtgaa ggtggaggca gcaatactaa cttcctgaat ttgaagagat gaattcactg
     3001 cattcagctt tacatgatcg agcaaactgt cggctttcaa ggtaatgacc agcttaccgt
     3061 cgaaagcatg cgagtaaggc gattggtagc acgaccagat atcaacatcg agttgatatt
     3121 tcaaaacatc gaacgacttg gggaaataat ttttctcttc gttgcgaaag tattcagtag
     3181 aatatttttt tattttatcg agatgacagc gatacgaccc tctttcgaaa acttgttgag
     3241 cttttccaga atttaatatt ccaaggccca aaacgaggga aagaattatg gtaagtttgg
     3301 ttttcataat ggttatgctt tgaatgggcg aagataggat ttttttcgaa gaaccaattt
     3361 tataaaatag agaaaagatt aatatggatg attctttatt ctttcgatgg ccgataaaat
     3421 ttcacggtga gagggaatag ccacattggt agcgagaaat ccttcataaa cgaaattgcc
     3481 atatttattg cattcggcga taaattcctt caccactgca ctcaggtcta tattcagagg
     3541 tacaagaata cgaagagagg tgtattcgaa caagaaacga agtttctcgg tttcaatctg
     3601 atagttttta catgtttctt cccattccat acggatgatg cgagcctggt tttcgctgaa
     3661 agttccttca aaggaaggga agatggcaat aaacatgcgc agtttgttgt ctttaccgcc
     3721 caaacccgtc gaaataaggc tgggaggatt gtcgctcaac tctccttcga ttaaggttcg
     3781 agaaatataa taggcaaaac gggcaaaatc ctgaagcttt ggtggagcaa cttccttgta
     3841 tttttcgata accctgaaag cttcgatgtt gcttcttaaa gcaagtatga caagaatttt
     3901 tttgccttcg gcaatagtta gatcgctctc gaataatcgg gcagccatct ttagggcatg
     3961 ttcatcgcca actttattgg gcttcagttt gcgtgcaagt cgtaaaaatt gctcctgatc
     4021 cttcacattg atgatatcat caacaattcg aatattagaa ctgtgttctg taatataatg
     4081 ataaaactct tcggcgtcgg aaaattctga aggttcgttc ggcatgtgag tcgatatttt
     4141 ttataatgga ataagaattc gtaacgaatg gggtttcact tttacatgaa cttctttgtc
     4201 cattttgaaa ggttctccat caatgtttac atatttgcct gtctcgcgaa ttacggtaaa
     4261 ttgatcggct ttataagtgt tcacatagag gctcttgtca attttatgcg taaatagaag
     4321 aaccaccgtg cgcaggattt ttgaataggg aatttttttg actattgtaa tatcggccag
     4381 tccatcggta atgctggcct tgggggcaat gaatgtattg tagccaaact ggttgctatt
     4441 agcaatgctt agtaatatgg ctctttcctt gatgatgaaa ttgttggcaa taacttggaa
     4501 gagtcgcggg cgaaaagtga tgtattcctt caccgcaatc ttgaaataag taaaaaatcc
     4561 tcgatgattg tctaaggcaa attgccttgc aatacgcgca tcgaagccca tacctgctat
     4621 gcttaccgca atacgatcgt ttagctgaat ggtgtcgatg cacatggctt ctccattttt
     4681 tatcaaattc aatgcttctt caagtttgaa aggaatcttg agaaaatgtg ccagaccatt
     4741 acccgaacca gccgggatga tgccaagaac agcactgcta tctaccagta cttgtgcaat
     4801 ttcgtttatg gttccatctc ctcctaccgc tacgatgata tcataacctt ttgcaacggc
     4861 tttttgcgta agatccgaaa ttctgtcctt ttcattaatt ctaacgattt ctgtttgaaa
     4921 ggtttcggcc ggaaaatatt cgggtatcat ctcttctaat ttttgtttac gcgaatgatg
     4981 aatgcctgca gcgggattga tgatgaagta gatctttttc atggttttac catttgatct
     5041 gcgcggagtc gatgatatat tatccggttg ttatattgct gtatgatagc ttttagaaaa
     5101 cgtaaagctt cgtcctgttc aggcaattgc tcagcagcaa ggttattggt ttgaagactg
     5161 tcgtttttta aatcgtagaa cccacttact ttttcattga aatatctgag gcatttattc
     5221 ttataaatta gttgatattc ttgatttaga tagctgatac caaatggggt agaggtgata
     5281 tcgaaaacac ttcgaccaaa ggcaacaaac gaaccctcag catttaagaa atccattacc
     5341 gaaggataaa tatcacattg ctgcgcaatc tgcgatgaaa agccgcggaa agaattatcg
     5401 ggcagataga agatcaaagg aatgacatgt tgcccaaccg gtgtaagata ataagggcta
     5461 aaagcctcgg aggtgtggtc ggctgtaatg atgaaaaggg tattcttgaa ccatgattcc
     5521 tgcgaggcca ttttgaagaa ttgacccaat gcatagtcgg cataacgtat gctttgatgt
     5581 atcttgagtt tcccaggtcc gaaacgattc atatattttg ctggaacgac ataaggatga
     5641 tgcgaagaca aactgaagaa tactaccaga aatggctgtg tttgattcga gagatccttc
     5701 gccatgaact ggaaaaaggg ctcgtcaaaa attccccatc ttccatcaaa ggcttctggt
     5761 cccctgtatt cattcttacc tttatattca tcgaaaccag acaaacgcga aaatgcatcg
     5821 aaatgcatgg tgccattcat tcctccatga taaaatatgg aatgatatcc cagaggcttc
     5881 aggcttaaag gcaggctcga gataggctcg ctaacatagg gcgaattgat gaattctacg
     5941 ggcataaggg aaggaaggct cgaacaaatg gcaggaatag cctcgatgga acgctttccg
     6001 ttagcatagg ctcgaaaagt aagaccatgc ctcatgaggg aatccagcac gggtgtgtaa
     6061 ccttccgtgg aatccttgta aagctgcgga ttcatcagtg cggaatgctc aagtgaaaaa
     6121 ctctccataa taattatgac cacattgaat ttcgttgtag gttgcgtagc tcgataataa
     6181 ttttttaggg gctggaatat gctggcggct tctgcttcat cttcataata aaccacttct
     6241 ttcagcgaag gttttccata ggttttgatg attgtaaaag gagtattcaa tataagagca
     6301 tcataatcgg gagaaatcat ttctgctgca ttcaccaaat agatcggcct gcgctgaaaa
     6361 cctcctctta ttcctacaat ggctattcct ccccatacaa tcattaaaaa caaattccag
     6421 ataaaaaagt tccatttgct ttggtaaaag agtttttttt cgtaccttag tcttgtggtc
     6481 agcaaaacaa aaatagaaaa cagaacaaag aaaaccagca gataaatcca gaagtcggat
     6541 aaaaattttg gaaggagcaa gcccatgttg tttgtggctt ttaaaaatcc agtaacatcc
     6601 catgtaagtc ttttcagcgt aaatggatag taagccgaat cgatgatatt gagaaggatg
     6661 ccaatggcgt ttgcgaaata aaataaaaag gaactaaaat ttcggtagta cttatgattg
     6721 cgaaaaggga aggggagggt tgcaaggaaa atataaggca aattcagcat gattacagcc
     6781 gaaagatcga atcgcaaccc taaccgccat attgtaaaaa tagaaggaat ggcttcggag
     6841 gggaaaagat catgctgaaa ccagtaaaga cataaacgcg aaagggaaaa aacaataaga
     6901 gctatgagta aacgataggc tacaataaga tgggtgccat ttttccagac gaatttaatt
     6961 ttcattcaaa aatacctggg cttattattt atttgtgata gatcagttgg cttatatacg
     7021 aagtgtcgag tgaagtggct gtttcggtta tcaaatggta aaaaagttga aaggattttc
     7081 catcgtgttg aatcgttcca gaagctaaaa aatacgtggt agaagtatcg gtaatctcaa
     7141 taatttgttt gctgaatcca agtgtatctc ccaagtatcc caacaaatcg gccttgatga
     7201 gagaagggta gaggcctccg atttgttcga agtaaatatg acgggttcca ggattataca
     7261 acgaatcagt gaacagaacc gaaatgcctc caggataagg cgaaattcgt tcacccgacc
     7321 atttgccggt taaatttgcc ccttcgatgc gaacaatgac cgttctttcg gcattggcta
     7381 cattattatt gaaatcttcg gcaaagtaat aaaccttgta tcggcccgcc ttgtgaatgt
     7441 tcaattcgga ggtatcaatc cacatcttga aaaccccata gttatcggtg ataataacac
     7501 ctgaatcata aaatgggctg ttgagtcgga tataaaccgg atttgatcct acaacggtga
     7561 taaacggagg ggtgtcatct tttacttcat ctatacaacc agtaagcgta aaacttgtga
     7621 atagacacgc acaaatacag aaaaacaaaa accttttcat aataagagga ttttgattga
     7681 ttttgcaaaa ttattcaaaa atcgatgact ttggttttta ctccgcctcg agtgtcaatt
     7741 aaggaatttt ggattacatc cattcgattg ctatcacggt tgttttgtaa ttttgctatg
     7801 aataatcgaa tcgaacagtc atgggtttac agtgtggaat tataggtttg gctggagttg
     7861 ggaaaactac tcttttcaat tgtttgtcga gttcaagagc tcgggcaggg ggaggcggaa
     7921 gtaaaagtaa tttaggaatg atttacgttc ccgatgatcg tttacaaaag atcgacgctt
     7981 tgataaaatc ggccaggatt gttcctacca cggtagatat cgtagatata ccgggtttgg
     8041 tgagaggagc tggtaaaggc gaaggtgtag ggagtaaatt tctggccgat atacgaaata
     8101 ccgatgcttt gattcatgtt ttgcgttgtt tcgatgaacc ttcggtgcct catgtggaaa
     8161 gcagcatcga ccctgtaaga gacatcgaga tggtagaact cgaacttatc gttcgtgacc
     8221 tcgaatcgat cgagaaaaaa ctacagcggc tcgagaaaca ggcaaagagc ggcgacaggg
     8281 aggctcgaac tggtatcgaa gtgctcgaag tatataaaca tcatttggag gattttcttc
     8341 ctgcacgcac agctcctgta ggaaaagaat tccaacgtta tttaaccgac ctttttctct
     8401 tgagtgcaaa acccgtcatg tatgtgtgta atgtcgatga cgattctgct attacgggaa
     8461 atgcttacag taatgccgta agagatttct tcgacgatca gaatgttgaa atattgacta
     8521 ttgcaggaaa attggaatca gaaattgccg aattggatga cccggccgat cgtcgggcat
     8581 ttttagagga ggtaggcttg aatgaacctg gcgttaataa attgatacaa tcggcatatc
     8641 gcctgctgaa tctccagact tttttcacgg caggacccaa ggaagtaaga gcatggacca
     8701 ttcgcaaagg aattacagca ccagaagctg ctggcgtcat tcattccgac ctcgaacgcg
     8761 gattcataag agctgaagtg atgaaatacg acgattttat tcgattgggc tctgaacacg
     8821 catgccgtga ggccggtaaa ctattcatcg aaggaaagaa ttatgtggtt caagatggcg
     8881 atatcctgca tatccggttt aatgtataag gtggcatcga ggcgtaaact gccttcatag
     8941 gaatcgaaaa ttattcgcag aatcttcccc ctgtccctga ttttatttct tgtttttcaa
     9001 tctgttggca acaaaataag aactcaggaa caaccctccc gaaagaatga aaggtaaggc
     9061 atacacaaat ccaccgaaag aaagactaag atgacctccc ataaattgaa gaaaaaatat
     9121 tacggccgaa aaaataagaa tgatgcccgc ttcaccagtg tgccaaaaac caatgataca
     9181 accaatgaaa gccattccca ctaataacat caaatcaatg atatggggaa gttttgtgcc
     9241 ttcggtaaac gtaaccctct ttattaaata aaataccagg aaaaataata aaccaacgat
     9301 tcctgtgatg aggccagcca ctttcagata tttgctcaat ttatggtttt tatgttcgta
     9361 catctgtctc gatcgaattt ttagtcaaac ccacaaacat acactatcga ttccaatcaa
     9421 atttgaccat cttcgaatga aataaaacga aacagccaaa atctaataca ctcgagcgaa
     9481 cctctgtcta ttcggataat catacagaaa gttttacgaa ttgataagat aattacccat
     9541 gaatatgttg acgaataaga atatgaaaga atcaagaccg ctgctaatgt gtaagttcga
     9601 acgagcttag ccttaaaggc atatctcaat tggtcgactt catgtttaac ttttaatcgg
     9661 aaagtacatg tgatagcagt cggtcttgac ttgtacaaaa ttacaatatt ttgaggaata
     9721 aacaacatga agaacacttt tgaagtgaag aaattttcga ccctaaggtg tggcattcta
     9781 tgggcaagta tttgagagag atgaaagcga acagaattgc catggagagt ccatgaattt
     9841 atgggatacc actagggaac ctattcgaag aaatgggatt tgagctgctt tttagtgaat
     9901 ccatatcgga taaaacaaat gccagataga acgagcgatg taaaagatgt gcaatggatt
     9961 gtcgtgtttt tacagagagg tatgttttaa cccaagtttg gttccggata agcatatcag
    10021 ggaattgcga acttatacac gaaaataagg catattgcaa aagtgtatca caagtgttct
    10081 gcaggaaatg gaatctgcct tggaaatgtg tggcattcgt attaccagct atgtaagcaa
    10141 tatatcgtcg aaaagtattc aaaagataat tgacagtatt gttgacggga gtgatgatat
    10201 ggatcaattg gaagaattgg cacacagtcg gattggcaat aagcataggg aacgaaaaga
    10261 agagatggaa ctattgtaaa ccatccgtgc gatgggaaaa atcatcttcc atgagtatca
    10321 tcgccgaaat ggaggcagaa tgagtaaatt cgggaacaac attaaattta gtggctgggc
    10381 atgattagcc catgaaatca tggaagtg
//
LOCUS       DAOORU010000081        10405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_7984_length_10405_cov_5.205411, whole genome shotgun sequence.
ACCESSION   DAOORU010000081 DAOORU010000000
VERSION     DAOORU010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10405
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7984_length_10405_cov_5.205411"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /locus_tag="PLF20_06310"
     CDS             <1..962
                     /locus_tag="PLF20_06310"
                     /inference="COORDINATES: protein motif:HMM:NF025229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOX80650.1"
                     /translation="FNGKIFVSVFDKKATIRTLGNDPNTTPMSFTIQNKRIAHTISKV
                     INGRFEVWFILPKDIEQNYGFGKFSYYATDSISDAAGAFIDFIIGGIDEVLATDTEGP
                     EIEIFLDDKNFISGNLSSPNPVLLASLADSSGINCFGYGLGRNISAWIDGQTSNAINL
                     DDFFVPLDPLNTRGNITYTFHNLENGQHTLTLRAWDLQGNSNQKTIKFTVAPNASIKL
                     WKITNHPNPFSDKTFFYFEHNQPGNSLRITIHIFNFQGIKVKTIRSKQSTSGYSIIPI
                     EWDGRGDSGELLPSGMYPYQIIVENNNGTISTQSQKLMIIRHN"
     gene            1094..2284
                     /gene="porV"
                     /locus_tag="PLF20_06315"
     CDS             1094..2284
                     /gene="porV"
                     /locus_tag="PLF20_06315"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HOX80651.1"
                     /translation="MPKFIKPTALALFTGLMCIPAILKAQDEYPITTAVPFLLIGPDA
                     RSGSMGETGVATDPDPVSIHWNAAKYAFIDKQMGFSVSYSPWLRQLVNDIGLGYLTFY
                     YRFDDKQALGASLRYFSLGEITFTDYIGNPKGTYKPNEFSLDAAYSRKLTDNLSGAVT
                     GRFIYSNLTLGQNVQGVESHAGTSFAADISLFYTKDLYINRNDAWFNFGLNISNIGAK
                     IAYTSTIEKDFIPTNLRFGPSFKYAFDDYNTLSFNIDITKLLVPTPPIYKLDSISGQP
                     IFDEQGNQVIEKGYNPNVSVFKGMIQSFYDAPGGFSEELKEITYAIGAEYLYNKQFAI
                     RAGYFHESATKGARQFFTFGAGLRYNVFSIDFSYLVSTDQRNPLDKTLRFTLNFDFEA
                     FRSH"
     gene            2495..4024
                     /gene="dnaB"
                     /locus_tag="PLF20_06320"
     CDS             2495..4024
                     /gene="dnaB"
                     /locus_tag="PLF20_06320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF004384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOX80652.1"
                     /translation="MDKNRPVEKKDRPKSLVDKPNSIKGQILDNVPGKIPPQALEVEE
                     VVLGALLLEKNALTSVIDFLKPEAFYREAHQHIFMAIKRLFAESQPIDLITVTNELRK
                     TGELEIIGGPYYLAQLTNRVASSANIEFHARIIAEKYIQRELIRVSTEIIHDAYEDSM
                     DALDLLDKAEMALFSISEENFRRDYEKMDSLVRKAIDEIRKAYEQEDALTGVPSGFTD
                     LDRITGGWQKSDLIVIASRPGMGKTAFILSMARNIALMKKRPVAIFSLEMSAIQLVTR
                     LISAESGLSAEKLRSGQLKDFEWEQLNVKITPLLEAPIYIDDTPSLSIFELRAKCRRL
                     KEQYDISIVMIDYIQLMSTGVENRGNREQEIALISRSLKALAKELDIPVIILSQLNRS
                     VETRGGTKKPILSDLRESGAIEQDADLVLFIYRPEYYKIETDEKGSTHNKADIIIAKH
                     RNGKTDEVRLQFIDKFARFTDDVDITQITSNLSMGVNYQGPTLTLPSKMNDDHPDDIP
                     Y"
     gene            4073..5335
                     /locus_tag="PLF20_06325"
     CDS             4073..5335
                     /locus_tag="PLF20_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOX80653.1"
                     /translation="MKKILTLCIILLILPLWALPMYLLVPMDEAQRNHLKGYGIAYLS
                     LTEGLEIDWLLNYRGGSFLIPYTASIDRECKLRGITTETLPDAQVAAILDEISRPEVN
                     MDALKLQKAPRIAVYSPKNKLPWDDAVTLALTYAQIPYDVIYDEEVIAGILPTYDWLH
                     LHHEDFTGQYGKFWARYKDYPWYKEDVRLNEETARKLGFNKVSKLKLAVALKIKDFVA
                     GGGYLFSMCSAPDSYDVALAAQNTDICDIMFDGDPIDPDAQSKLDYSQCFAFTDFTIS
                     TNPYEYEISSIDVDPNQRGLNEQNDYFTLFDFSAKWDPIPTMLCQNHTRIIKGFMGQT
                     TAFRKQYVKPDVLIMGENKEANEARYIHGDFGKGTWTFLSGHDPEDYQHLVGDPPTNL
                     DLYPNSPGYRLILNNVLFPAAKKKKHKT"
     gene            complement(5332..6228)
                     /locus_tag="PLF20_06330"
     CDS             complement(5332..6228)
                     /locus_tag="PLF20_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80654.1"
                     /translation="MLHFSCHSWKFILCFRKDKILILIAMAILCLTACKDKEEYRFDT
                     SYNKQAISDFLTADAIYRDLFRQAYQLSFDTSILKKGEGIIDSAIVKKQGEEWIFTYD
                     KEQNCPDGSKRSGQFRIHWNAPLSDSNAQLTIFPQGLTINEKRIGGQINITRISKPKV
                     FYRVKVDTGSISLVVAGYSKKQRIQYSSNYKYYWQKGSQTPYDIYDDVLYMYGIASGR
                     GREFDRFIYVTLDTLTYRFICHYPRGGKALFQMPDFEINQMDLDYQKANECSAVVKAT
                     LWGLLPNGSKTKDEVVYFTISY"
     gene            complement(6238..6735)
                     /locus_tag="PLF20_06335"
     CDS             complement(6238..6735)
                     /locus_tag="PLF20_06335"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOX80655.1"
                     /translation="MEKKPLTIIVAIANRNAIGLNNQMLCHLPDDLKRFKRITMGHNI
                     IMGRKTWESLPKKPLPGRNNIVLSRRVLQLEGATVVSSLEQALDLCPLNEESFVIGGG
                     ALYRLFLPYVDKLYITRIYHDFEADTFFPDFDEKEWQLVMQEDVFPNETNPYRYSYLT
                     YNRKH"
     gene            complement(6736..7530)
                     /locus_tag="PLF20_06340"
     CDS             complement(6736..7530)
                     /locus_tag="PLF20_06340"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOX80656.1"
                     /translation="MKQYLELLRHILATGTEKHDRTGTGTISVFGYQMRFNLEDGFPL
                     LTTKKLHVRSIIYELLWFLRGDTNIRYLHENKVTIWDEWADANGDLGPIYGYQWRSWP
                     TREGKSIDQISQVIDSIKQNPDSRRHIVSAWNVEEIDKMALPPCHIFFQFYIANNKLS
                     CQLYQRSADVFLGVPFNIASYALLTLMTAQITGYQPGELIHTLGDAHIYKNHIEQAKL
                     QLTREPFPLPTMILNPEIHSIFDFRYEDFQLINYRAHPHIKAEISV"
     gene            complement(7540..8124)
                     /locus_tag="PLF20_06345"
     CDS             complement(7540..8124)
                     /locus_tag="PLF20_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOX80657.1"
                     /translation="MMKRKPFALISFLLIVISACIRPQYDNFSSIGEDHYLRMVIETP
                     AGNLNCVHFHPSSNSFIQGNAFQKPAPLNFGFIPSSNRSKSAQEFDPIRVMLLSNSKP
                     SGTILKIIPIGMLQFAASGKQRVLILAVPADPSHRKSNINDEAAISMTEPSIFSILEL
                     WLKNYSAIPDVIIYGWADENITWNYILDVLRLKS"
     gene            complement(8121..9380)
                     /locus_tag="PLF20_06350"
     CDS             complement(8121..9380)
                     /locus_tag="PLF20_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HOX80658.1"
                     /translation="MVVLLAISYFLSMNRKAIEWKVVLTGLAIQIFLAIGVLYIPAIR
                     IIFEVGGKIFVEILNFTKAGSEFLLGKLLDANSFGFIFAFQVLPTIIFFSALMSLLFY
                     LGIVQIVVQGLAWLLAKALKISGAESLCAAGNIFLGQTEAPLMIKPYLPSMSRSEIFL
                     VMTAGMATMAGGVLAAYISFLGGDDPVQRLVFAKHLLSASVMAAPGAVVVSKMLVPQT
                     ESVDKTVKIPKERIGNNVLDAISNGATEGLKLAANVAAMLLVFVAFIAMANFILNDLI
                     GHYLGINDWIANQTSGRYEGLTLQFILGYSLAPLMWLIGVNIHDITLVGSLLGEKIIM
                     TEFVGYISLANYKAAGLFFEYKSIIISTYLLCGFANFASIGIQIGGIGSLAPNQKSQL
                     AAFGFRALIAGTLSALVSSTIIGMITL"
     gene            complement(9627..10208)
                     /locus_tag="PLF20_06355"
     CDS             complement(9627..10208)
                     /locus_tag="PLF20_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005640152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HOX80659.1"
                     /translation="MKKVQMEIAGMSFSQVQSGAYALILGEIGGKRRLPIIIGSAEAQ
                     AIAIELEKMKPARPLTHDLFKTFAETFGVRVIEVFINKFIEGVFHAVLVCDDGHNIVE
                     IDSRTSDAVALALRFNCPIYTTEEILDAAGIVIDENALIEEEHSHEAGEEENLLGWEA
                     YSDEELEQMMQEAIENEDYERASKIRDELKQRK"
BASE COUNT         3179 a         2253 c         2015 g         2958 t
ORIGIN      
        1 acttcaacgg aaagattttc gtctcggtct tcgataagaa agccactatt cggacgcttg
       61 ggaacgatcc gaatactaca cccatgtctt ttacgatcca gaataagcga attgctcata
      121 ccatctctaa agtaataaat ggaagattcg aagtttggtt tattctacca aaagatatag
      181 aacagaatta tgggtttgga aaattcagct attatgccac ggatagtatt agtgatgcag
      241 ctggagcatt tattgatttc atcataggcg gaatagacga agtcttggct accgatactg
      301 aaggacccga aatcgaaata tttctcgacg acaagaactt tatttcggga aacctctcga
      361 gccctaatcc tgttctgctg gcttctctgg ccgattcttc cgggattaac tgtttcggtt
      421 atggattagg acgcaacatc tctgcatgga tagatggaca aaccagtaat gccatcaatc
      481 tcgacgattt cttcgtccca ctcgacccat tgaatactcg cggaaatatc acctatactt
      541 tccataacct cgaaaatgga cagcacacac ttacgctgcg ggcctgggat cttcagggaa
      601 atagcaacca aaaaaccata aaatttaccg tagcacccaa cgcttctatc aaattatgga
      661 aaatcaccaa tcatcccaat cctttcagtg ataaaacctt cttttatttc gaacacaatc
      721 aaccgggaaa ttccctgcgg ataactattc atatcttcaa cttccaggga atcaaggtaa
      781 aaaccataag gtcgaagcaa tctacttcgg gttatagtat aatccccatc gagtgggatg
      841 gaagaggcga ttcgggagaa ttacttccat cgggaatgta tccttatcag attattgtag
      901 agaataataa tggaactata agtacgcaaa gccaaaagtt aatgattatc aggcataatt
      961 agggaaaaac caattctgaa tcaatcctcc aattcttaat cccttgtgaa tgaaaaatac
     1021 tataacatat cttttctcgt tacttttgca gctttatttt caattcatcg aaatataaaa
     1081 caaaatacct tatatgccga aattcattaa accgactgcc ttggctctgt ttacagggtt
     1141 gatgtgtatt cctgcaatat tgaaagctca ggatgagtat ccgattacta ctgccgtacc
     1201 ttttctttta ataggacccg atgcgcgttc tggttcgatg ggtgaaacgg gcgttgcaac
     1261 cgatcccgac cctgtttcta ttcattggaa tgcagctaaa tatgccttca tcgacaaaca
     1321 aatgggattt tcggtttcct attccccatg gctacgtcaa ttggttaacg atattggact
     1381 cggttacctc acattttatt atcgctttga tgataaacag gccttaggag cctcacttcg
     1441 ctatttttct ctgggagaaa tcacatttac cgattatata ggtaatccca agggcacata
     1501 taagcccaat gaattttcgc tcgatgctgc ttattcacgt aaattaaccg ataacctctc
     1561 tggagcggta actggccggt tcatttattc gaatctaacc ttaggccaaa atgtacaggg
     1621 agtggaatcg catgcaggaa caagttttgc cgctgatata tccttgtttt acactaaaga
     1681 cctgtatatc aaccgcaatg acgcatggtt caatttcgga ctaaatattt ccaacattgg
     1741 agctaaaata gcctatacct cgactatcga aaaagacttc attccaacca atctgcgctt
     1801 cggaccaagt tttaaatatg ctttcgacga ttacaatacc ctctccttca acatagatat
     1861 tacaaaactt ctggttccta cgcctcccat ctacaaactc gacagcattt cgggtcaacc
     1921 catattcgac gaacaaggaa atcaggtgat cgaaaaaggt tacaacccta atgtatctgt
     1981 cttcaaagga atgatccaat ccttttatga tgctccaggt gggtttagcg aagaattgaa
     2041 agaaataact tatgctattg gtgctgaata tttgtacaac aagcaatttg ctatacgagc
     2101 gggctatttt catgaatccg ccaccaaggg agctcgccag ttcttcacct tcggcgctgg
     2161 cttacgttac aacgttttta gcatcgattt ctcctatctt gtgagcactg accaaagaaa
     2221 tcctctcgat aaaactctgc gtttcactct taatttcgac ttcgaagcat tccgttcgca
     2281 ttgagataat taactcgatc cgaacaaggg ttcgttaaac tcataataca actcgccggt
     2341 tcaagacaag gaaccggttt tttcataata accagaaaat ctattcgact ttagtttcag
     2401 gggggataat ttttttataa agtcgaaaat ttgaatacaa aaagaattat ttggaaaagg
     2461 gcttttcctc ctcgagtttt tgtaaatttg aaacatggat aaaaacagac ctgtagaaaa
     2521 gaaagaccgc cccaaaagcc ttgtagataa accaaatagc ataaaggggc aaattctgga
     2581 caatgtacca ggaaaaattc cacctcaagc cttagaagtg gaagaagtag tgcttggcgc
     2641 attattactc gagaaaaatg cattaacgtc ggtgattgat tttttaaaac cggaggcatt
     2701 ttacagggaa gctcatcaac acattttcat ggcaatcaaa aggctgtttg ccgaaagtca
     2761 gcccatagat ctgataacgg ttaccaacga actgcgaaaa accggagagc tggagattat
     2821 cggaggccca tattatttag ctcagttgac gaatcgtgta gcctcttcgg caaatattga
     2881 atttcatgcc cgcatcatag ccgaaaaata tattcaaaga gaattgatca gggtttcgac
     2941 cgagattatt cacgatgctt atgaagattc gatggatgcg ctcgatttgc ttgataaggc
     3001 cgaaatggcc ttgttttcaa tcagcgagga aaatttccgg cgcgattacg aaaaaatgga
     3061 ttctttggtg cgcaaggcca tcgatgaaat tagaaaagcc tatgaacagg aggatgccct
     3121 aacgggagtt ccttcgggat ttaccgatct ggatagaatc accggaggtt ggcaaaaatc
     3181 tgacctcatt gtaatcgcct caaggcctgg catgggaaaa acggcattta tcctaagcat
     3241 ggctcgcaac atagctttga tgaaaaaacg tccggtggcc atattctctt tggaaatgtc
     3301 ggccatacaa ctggttacac gtcttatctc ggccgaatcg ggtctttcag ccgaaaaact
     3361 ccgatcggga cagcttaagg atttcgaatg ggaacaactc aacgtaaaaa ttactcctct
     3421 gctcgaagct cccatttata tcgacgacac cccctccctc tccatattcg aactaagggc
     3481 aaaatgccgc cggctgaaag aacaatacga cattagtata gtaatgatag attacatcca
     3541 gttgatgagc acaggagtgg aaaatcgcgg aaaccgcgaa caggaaattg cattgataag
     3601 ccgttctcta aaagccttag ccaaagaatt ggacattcct gtcatcattc tttcacaatt
     3661 gaatcgcagc gtcgaaaccc gtggcggaac caaaaagccc atcttgagcg acttgcgtga
     3721 atcaggagct atcgaacaag acgccgatct cgtactgttt atctatcgtc ctgaatatta
     3781 taaaatcgaa accgacgaaa aaggctctac acacaacaag gccgatatca tcattgccaa
     3841 gcatcgaaac gggaaaaccg acgaagttcg attgcaattt atcgataaat ttgccagatt
     3901 tactgacgat gtcgacatca ctcagattac ttccaacctc tccatgggag taaactatca
     3961 agggcctacc ctgaccctgc cctctaaaat gaatgatgat catcccgacg atatccccta
     4021 ttagcacttc tttcccttat attataataa tccggttatt tcttcaagaa agatgaaaaa
     4081 aattctgacg ctttgcatta ttctattgat attaccttta tgggccttac ccatgtatct
     4141 gcttgtgcct atggacgaag ctcagagaaa ccacctcaaa ggctatggca ttgcttatct
     4201 atccttgact gaggggcttg aaatagattg gttgctgaat taccggggag gaagtttctt
     4261 gataccttat actgcttcca ttgatcgtga atgcaaacta agaggtatta ctacggaaac
     4321 cttgcccgat gctcaggttg cggccattct cgacgaaatc tcacgacctg aagtaaatat
     4381 ggatgccctt aaacttcaga aagcccctcg catagcagtt tattctccca agaataaatt
     4441 accatgggac gatgccgtta cgcttgctct cacttatgcc caaatcccct acgatgtaat
     4501 ttacgatgaa gaagttattg ccggaatcct tcctacctat gattggttac accttcatca
     4561 tgaagatttt accgggcaat acggaaaatt ctgggcaaga tacaaggatt atccatggta
     4621 taaagaagat gtaaggctga atgaagaaac ggcaagaaaa ttaggtttca ataaggtctc
     4681 gaaactcaaa ctagctgtag cgcttaagat taaagacttc gttgcgggag gtggctatct
     4741 gttttcgatg tgttccgcac ccgatagtta cgatgtagct cttgcggcac agaataccga
     4801 tatttgcgac attatgttcg atggcgatcc catcgatccc gatgcccagt cgaaactcga
     4861 ttacagccag tgtttcgcct ttaccgattt taccatttcg accaaccctt acgaatatga
     4921 gatctcatcg atagatgtcg atcccaatca gcgaggtctt aacgaacaaa acgactattt
     4981 cacgcttttc gatttctccg ccaaatggga tccaataccc accatgctct gtcagaacca
     5041 tacacgaatc atcaaaggat ttatgggtca aactacagct ttcagaaaac agtatgtaaa
     5101 gcccgatgta ttgattatgg gggaaaacaa ggaagccaat gaagcgcgtt atattcatgg
     5161 agattttggc aaaggaacct ggacattctt aagtggccac gatcccgaag attatcaaca
     5221 tctggtaggc gaccctccta ccaatctcga tctctatccc aattctccgg gttaccgcct
     5281 gattctgaat aatgtcctgt tccccgctgc taaaaagaaa aagcacaaaa cctaataact
     5341 aatcgtaaaa taaaccactt catccttggt tttcgaacca tttgggagta atccccaaag
     5401 agtagccttt accactgccg aacattcatt ggctttttga taatcaagat ccatctggtt
     5461 tatttcaaaa tcgggcatct gaaataatgc ctttcctcct cttggataat gacatataaa
     5521 tcggtatgtc aaagtatcga gagttacata aataaaacga tcgaactctc ttccccggcc
     5581 cgatgctatg ccatacatgt acaaaacatc gtcataaatg tcataaggcg tttgagatcc
     5641 tttctgccaa taatatttat aattactact atactgaatc ctttgctttt tcgaataacc
     5701 agccacgacc aacgagatag aacccgtatc gacttttact ctgtaaaaaa ccttgggttt
     5761 actgatacgt gtaatgttga tctgcccgcc tattcgcttt tcattgatgg ttaaaccttg
     5821 tggaaaaatg gtaagctgtg cattggaatc gctcaaaggt gcattccagt gtatccgaaa
     5881 ttgtccgctt cgcttcgaac catcagggca attctgttct ttatcgtacg taaaaatcca
     5941 ttcctctccc tgtttcttta ctatagccga atcgataatc ccttcaccct ttttcaatat
     6001 agaagtatcg aacgataact gataagcctg gcggaacaaa tcgcgataaa ttgcatcggc
     6061 cgtaagaaaa tcacttatgg cttgcttgtt ataagaagta tcgaacctat attcttcttt
     6121 atccttgcat gccgttaaac atagaatagc cattgcgatt agaatgagaa ttttatcctt
     6181 tctaaagcaa agaatgaatt tccatgaatg gcacgaaaag tgaagcatag catgaattta
     6241 atgttttctg ttataagtta aatacgagta tctatatgga ttggtttcgt taggaaaaac
     6301 atcttcctgc attacgagtt gccattcttt ttcgtcgaaa tcggggaaaa aggtatcagc
     6361 ttcgaaatcg tgatagattc gagtgatata gagtttatca acataaggca gaaaaagtcg
     6421 atagagagcc ccacctccta tgacgaaact ttcctcattt aaagggcata aatccagtgc
     6481 ctgttcgagg gaagagacca cagtagctcc ttccaattga agtactcgac ggcttagaac
     6541 aatattgttg cggccaggta agggcttttt gggcagtgat tcccacgttt tacgacccat
     6601 tattatgtta tggcccatgg taatccgttt gaaacgtttc agatcgtccg gcagatgaca
     6661 gagcatctgg ttattcaggc cgatggcatt gcgattggcg attgcaacta taatagtgag
     6721 aggtttcttt tccatctaaa ccgatatttc agcttttata tggggatgag ctcgataatt
     6781 gatcaattga aaatcttcat atcgaaagtc gaaaatagaa tgaatttctg gattcaaaat
     6841 catagtagga agtgggaagg gttctcgtgt aagctgcagt tttgcctgtt cgatatgatt
     6901 tttatatata tgggcatcac ctagggtatg aatcaattct ccaggttggt atcccgttat
     6961 ttgagccgtc atcagggtta gcaaggcata cgaagcaata ttgaagggaa ctccaagaaa
     7021 tacatctgca cttcgctgat agagctggca actcagttta ttgttagcga tataaaactg
     7081 gaaaaatata tggcagggag gcaaggccat tttatcgatt tcttctacgt tccatgcact
     7141 aacgatatgc cggcgagaat ctggattttg ctttatcgaa tcaatgactt gactgatttg
     7201 gtcgatggat ttgccctctc tggtaggcca tgatcgccat tgataaccat aaattggtcc
     7261 aagatcacca tttgcgtcgg cccattcatc ccagatggtt accttatttt cgtgcaggta
     7321 tcgaatattg gtatctccac gtaaaaacca gagcagttca taaataatac tgcggacatg
     7381 aagtttttta gtagtaagca atggaaatcc atcttcgaga ttgaaacgca tctgataacc
     7441 aaacacgctg attgttcccg tacccgtacg gtcatgtttt tcagttccag ttgcgagaat
     7501 atgacgaaga agttcgagat attgtttcat agattcactt taagatttta ggcgaaggac
     7561 atccaaaata taattccaag ttatgttttc gtcggcccat ccatatatga taacgtcggg
     7621 gatagcagaa taatttttca gccataattc gagaattgaa aaaatagaag gttcagtcat
     7681 gcttattgca gcctcatcgt ttatattgga ttttctgtgg gaaggatcgg ccgggactgc
     7741 aaggatcaaa actctttgct ttccactggc cgcgaattgc aacatgccaa tgggaataat
     7801 tttcaatatg gttcccgaag gtttagaatt tgataacagc attactctta taggatcgaa
     7861 ttcttgagcc gacttcgatc tattggacga tggaatgaag ccaaaattaa gaggcgcagg
     7921 tttctgaaaa gcatttccct ggataaaact gttgctcgaa ggatgaaaat gaacgcaatt
     7981 aaggttcccg gctggagttt ctatcaccat tctcaaataa tgatcctcgc ctatggacga
     8041 aaaattgtca tactggggcc ttatacaagc actaatgact attagaagga atgaaattaa
     8101 agcgaaaggc tttctcttca tcataatgta atcattccaa tgatagtgga agaaaccaat
     8161 gcagacaatg tacccgcaat taaagcacga aacccgaagg ctgcgagttg gcttttttga
     8221 ttgggggcaa gagagcctat cccgccgatt tgtattccaa tagaggcaaa attagcaaaa
     8281 ccacaaagca ggtaagtaga aataataatg ctcttatatt cgaagaaaag acctgcagct
     8341 ttatagttag caagactaat atatccaacg aattccgtca taataatttt ttcgcccagc
     8401 aggcttccta ccagtgtaat atcatgaata ttgactccaa taagccacat caaaggagcc
     8461 agagagtacc caaggatgaa ttgaagggtc aaaccctcgt agcgtccgct ggtttggttg
     8521 gcaatccagt cgtttatgcc caaataatgg ccaatcaaat catttaaaat aaaattggcc
     8581 atggctataa aagcaacaaa tactagcagc atggccgcaa cattggctgc tagtttcagc
     8641 ccttcagtag ctccattcga gatggcatcg agcacattat taccaatacg ctcttttgga
     8701 attttaacag ttttatctac actttcggtt tggggtacca gcattttact gacaacgacg
     8761 gcacctggag cggccattac cgaagccgaa agtagatgtt tagcgaagac gagcctctgg
     8821 accgggtcat cgcctcctaa aaagctaatg taagcagcca gcactcctcc cgccatcgta
     8881 gccatcccag ccgtcatgac cagaaatatt tccgaacggc tcattgaggg aagatagggt
     8941 ttaatcatga ggggagcttc ggtttggccc agaaatatgt tgccagcagc acacaggctc
     9001 tcagcacccg aaatcttcaa ggctttggcc aataaccatg caagcccttg caccactatc
     9061 tgcacaattc ctaaataaaa caaaaggctc attaaagccg aaaagaaaat aatggtcggt
     9121 aaaacttgaa aagcaaaaat aaaaccgaag gaatttgcat cgagcagctt ccctaaaaga
     9181 aattcactcc ctgctttggt aaaattcaaa atctcgacaa atatcttacc acccacttcg
     9241 aatataatac gtatagcagg aatatataaa acaccaatag ctaaaaaaat ctgtatggcc
     9301 aatccggtaa gaaccacttt ccattcgatg gctttccgat tcatgctcaa aaaatacgat
     9361 atagccaata aaacaaccat gcccaggagc cctctcagaa cggaggttag tgaaagaccc
     9421 agtttgagtt cctcaggagc atgagtgatt actgagtttg ctccttgcaa ggctgtatcg
     9481 gctaaagata gctgtacatt cagggtatca ggcgtattta ttacctgcgg cattcctata
     9541 attggtaaaa aacaaatcga taatgtagcg agaatcagtt tgatggtagg atttttcata
     9601 ggaattatat gacaattagg ttagtcttat tttctttgtt tcagttcatc cctaatctta
     9661 cttgctcttt cataatcttc attttcaatg gcttcctgca tcatttgttc taactcttca
     9721 tcggaatatg cctcccatcc caataaattt tcttcttcac ctgcttcatg tgagtgctct
     9781 tcttcaatca gtgcattttc atcgatcact attcctgcgg catcgagaat ttcttcggta
     9841 gtatagatag ggcaattgaa tcgcagagcc aaagcaacgg catcggaagt cctcgaatca
     9901 atttcaacaa tattatgccc gtcgtcgcat accaagacag catgaaaaac tccttcgata
     9961 aatttattga taaacacctc gatcacccgt acgccaaaag tttcggcaaa agtcttgaat
    10021 aagtcatggg ttaatggcct ggcgggtttc attttttcca gttcgatagc tatagcttga
    10081 gcctcggcac ttcctataat gatgggtaaa cgcctttttc ctccaatttc acctaaaatc
    10141 aaagcatacg caccactttg tacttggcta aatgacattc cggcaatctc catttgaact
    10201 ttcttcatcc ttaatttact atatttatta ttgtaaatca gtcgattaat ataatatttt
    10261 aatatgtata ttcccttaaa aacttcatgc tctcatcaat ttatatctgg catacaaaat
    10321 aaagcaaatc ttacgaaaaa ttggttatat atattttttc actattaacc gacaaaattc
    10381 taaaagttcc taaacatttt ttata
//
LOCUS       DAOORU010000082        10366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8019_length_10366_cov_5.118320, whole genome shotgun sequence.
ACCESSION   DAOORU010000082 DAOORU010000000
VERSION     DAOORU010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10366
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8019_length_10366_cov_5.118320"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..792
                     /locus_tag="PLF20_06360"
     CDS             310..792
                     /locus_tag="PLF20_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80660.1"
                     /translation="MKALTYSIFVLLISLFSVSSLNAQVVQINQTFSSDSIFTPFSGT
                     APVYSMNFSGGINLLSDSSLVWVVLIDTYGNHFLVFESYPLITLENAFDTLWASDETT
                     YPGAGARNILIASDLLDYHEPIVSETKMKSSWRDRFYWTYTRPIMSDLKFFLILQKII
                     "
     gene            1013..1504
                     /locus_tag="PLF20_06365"
     CDS             1013..1504
                     /locus_tag="PLF20_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80661.1"
                     /translation="MKFRILFSGIVFLSALSNIYGQWPVIYEDSINVVPDRVWEHYDK
                     GYIFTGEKYLGWSYYGWINKTDINGEILWEKSYGNPSDRTKFSSSRITTDGGLIISGG
                     SNKLSSSCIDPIIVKINACGEKEWCKIYSAQWCNSWAQDIEILPDGGYLALINRWNNS
                     EED"
     gene            1529..2233
                     /locus_tag="PLF20_06370"
     CDS             1529..2233
                     /locus_tag="PLF20_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80662.1"
                     /translation="MGDIIWPQTYATDTAFFSESPHSLLKTQDSCLVITGEAYYPDST
                     YPGHWIQKIILIKVKQNGEVIFEVPWGSNNGIYCDGRASTIDAANNIYTAGRRARTMA
                     PSGDSPCLFKTSATGYPVFYNDLKETSTMGIATTINWFQDSTLVQCMYWGSHVGVDTT
                     GLVNTDSFGTIIKEKPLLINYGTGAFWGSDITFNNRLILAGSVYSGSTFSVYLYKVNF
                     DLEYDSIYTTPFTYDN"
     gene            3499..4584
                     /locus_tag="PLF20_06375"
     CDS             3499..4584
                     /locus_tag="PLF20_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80663.1"
                     /translation="MKICKRCILNENYPGITFNDDSICSLCISKTDFIPKGEEKLIRI
                     FEKARKRNKKYDALVPLSGGKDSTYILHLAVNIYNLKVIAMTYDNGFMSELSLDNIKN
                     AVDITKVDHVFYKPDQELQKKIYRIMLLKTGDICGACDIATRACILKASLEYDVPIVL
                     YGTSPLEDNSFVPDSIEDIGRFKYVLISSGEINDKEIKDFTIYPWLNDLILSFNKLVG
                     IYPREVRPLFYIKNPSDKEMGEIISKELKWRDDGREYSRHLDCIAEPFTNYIRNRIYG
                     YERRICQYSNMVRNNEISRAHALELYEDDKIDQPPENYIEILNYLGLNEKDLDQILSY
                     KPLMYEDYLSRMNRIYQWLAKFKRFLQ"
     gene            4594..5436
                     /locus_tag="PLF20_06380"
     CDS             4594..5436
                     /locus_tag="PLF20_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX80664.1"
                     /translation="MKIISIIAENWKADGGAAFGVVPKTIWGKLYPADDDNLQKITSR
                     CLLVDTGSRVILFDTGMGNKQDEKFFRYRYRFGDDNLFENLKYAGYSADDITDAVFTH
                     LHFDHCGGASCWNSEGSKVVRVFPNATYHVSKAQAQWALYPNKREAASYLKENLEPIF
                     EAGPLNLIEKPGPFIQQIDLRFYNGHTSGQIIPFIHLNNQTVVFVSDFIPSSAHLPLV
                     YIPAYDIQPLITLTEKEEFLHEAIEKQYILVFGHDYFNEAATVIIKGKGFQAEKIGKL
                     SEFI"
     gene            complement(5579..6967)
                     /locus_tag="PLF20_06385"
     CDS             complement(5579..6967)
                     /locus_tag="PLF20_06385"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOX80665.1"
                     /translation="MKNHISKFILISVISLLSYSCLGQTSISGKIEGAAGMKLGIAYY
                     YGENLRAFDSLRCNENGEVNLVVPKYIPPSQMCILTQQNDGFQFFYDGKSLTFHTNIS
                     ALQDSLNFIDSKGNTDFLKFTKYEVRYEKIKELLENMLDEYPHELPFYQEIIDEYNLR
                     QIDYEATLNKIITENQENLAGKYLMTRRKPFVSPNLLGEERKNFLKQHFFDKISFDDK
                     GLLYTNAYPRLVIEYLELYANPNFDKKKLEISLIEGIKIIMSILQPYAEAYDMITEYL
                     LDGFQNFGFERVVQFISERYEQGNCINTNPHLTSRIEISKRLSPGSPAPEIKGKDLRD
                     HEIKLSNYKGNYVLLIFWGSWCNHCSALLPKIASLYARPHQKKWEIITVVIDTNLRAV
                     EDSVRNNHYTWPVIADGKGWDTPPAIDYGINVTPAMFFIGPDLNIIAKPSDFSEVLDL
                     ALTYGLIKINSH"
     gene            7207..7911
                     /locus_tag="PLF20_06390"
     CDS             7207..7911
                     /locus_tag="PLF20_06390"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HOX80666.1"
                     /translation="MDESEVLILRSKMENLTEDAALKEVAEWFPSRVCFASSLSAEDQ
                     VILHLIATQNLPIRIFTLETGRLFEETYDLLEKSRLRYKINIEVIFPDYKRVEKLVNE
                     KGINLFYYSVENRKMCCSVRKVEPLRRALEGMKLWITGLRRQQSQTREHTQLLEWDEV
                     NGLLKFNPLIAWTDEKLWNFIHDHQIPYNPLHDKGYPSIGCEPCTRAIQPGEDVRAGR
                     WWWEAQGGRECGIHRS"
     gene            complement(8697..>10366)
                     /locus_tag="PLF20_06395"
     CDS             complement(8697..>10366)
                     /locus_tag="PLF20_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOX80667.1"
                     /translation="RQNDIEGHGGIEDGNWISGIKFIDQARLGNQENYPEYRKQNPAY
                     NRFYFLPLILGLIGLFYHIKKHPRDSFVVALLFFMTGIAIVIYLNQTPFQPRERDYSY
                     AGSFYAFTIWVGLGVLALNDWLGKYIKPVAAAILAILIALPVPYVMAKDGWDDHNRSH
                     KTAARDYAFAYLDSTQPQSLLFTFGDNDTFPLWYAQEVENHRTDVRVVNYMLASGDWY
                     IHQMARKVYDSEPLPFTLKPEQYDKGNNDYVVVVDLNKSGQPILLRDIMRFIADERNK
                     RDFGDGQRLSFIPSKTIRIPVDTAAVKASGLIPNEMKNRVLPYLDFTIRRNYIFKNDL
                     MLLDILATNDWKRPIYLTSPSGIGGAINLDEYSHLEGMVYKFLPVKATDYIRGLGGVS
                     ADTCYDILVNRIKHWGNLNDPRVTVDRESFRNAAIPRQNYMRVAQSMLNKGKNEEAEK
                     ALDLSLQYFPTSKIFPDKYMLSYVDLYYAAKATEKANNMALQLANIFSQDLNFYLSLE
                     PKYSSQYEEEMSENAYLLQRLSQVASQNGQDSTAKVIEAMINLKLSQ"
BASE COUNT         3277 a         1802 c         2053 g         3234 t
ORIGIN      
        1 tacaggttaa aattaatgat ggactcaatt tactgctttt acataggtgc tttgcatcag
       61 gcgggctgac gtgcaaactt ggagcttcgt gcttctattt aagttcagtg gtggttgaca
      121 gttttgtgct ccgaactgaa agtcagcgaa gctaaaccca ccactgcgcc aagcgccaaa
      181 acgttaatta caaaaaaatc tattggttta tgctgaatat gatgttttaa taaatttaga
      241 aaactcatat actatcctga agaatccaga taatagatta tatttacagt gttcaaatat
      301 taaaaagcca tgaaagcgct tacttattca atctttgttt tgctcatctc actatttagc
      361 gtttcatccc ttaatgcaca ggtcgttcag atcaatcaga ccttttcttc cgacagcatc
      421 ttcaccccct tttcaggaac agcgcctgtc tattcaatga acttttcagg tgggataaac
      481 ctgctcagcg actccagcct agtatgggtg gtcctgatcg atacatatgg gaatcatttc
      541 ctggtttttg aatcttatcc gttgatcacc cttgagaatg cctttgacac cttgtgggcc
      601 tccgatgaaa caacttatcc tggggctggg gcaaggaaca tactgattgc atctgattta
      661 ctggattatc atgaaccaat tgtcagtgag accaagatga aatcatcttg gagagaccgt
      721 ttttactgga catatacacg accaattatg tctgatttaa aatttttcct aatcctgcaa
      781 aagattatat aattactgaa tatcgaaaga atgattctaa cgaggaagtt attctcgaaa
      841 tgattgattt taatggtaaa atagtgcgat cctataattt gccaaaaact gaaaaccaac
      901 agttgatctc tttacaaggt ctttccgggg gcacatattt tatcaatcta tataaaaata
      961 attcctttaa aggagcctat aaagttattg taatccgcta atttacttac ttatgaaatt
     1021 caggattctt ttttcaggga tagttttcct ttctgcatta tccaatatat atggccaatg
     1081 gcctgtaatt tatgaagatt cgattaatgt tgtgccagac cgggtatggg aacattatga
     1141 taaaggctat atttttacag gagaaaaata tcttggctgg tcatattacg gatggataaa
     1201 taaaacagat attaacggag aaattttatg ggaaaaatca tatggaaatc catctgatag
     1261 gactaaattc tcatcgtcac gaattacaac tgatggggga ttgattatat caggaggatc
     1321 aaataaactt tcttcaagtt gtattgatcc aattatcgtt aaaataaatg cctgtggaga
     1381 aaaagagtgg tgtaaaatct atagcgccca atggtgtaat agctgggctc aggatatcga
     1441 gatccttccg gatggtggtt atttagcatt gatcaatcgt tggaataata gtgaagaaga
     1501 ttgaatctgg ttattcaaac tggatagtgt gggtgatatt atttggccac aaacgtatgc
     1561 aacggataca gctttttttt ctgaaagtcc acattcctta ttaaaaacgc aagattcttg
     1621 cttggtgata accggagaag catattatcc agattccact tatcccgggc attggattca
     1681 aaaaattatt ctgattaaag taaaacaaaa cggagaagta atttttgagg ttccctgggg
     1741 atcaaacaat ggcatatact gcgatggccg ggcctctacc attgatgcag ccaataatat
     1801 ttatactgcc ggaagaaggg caagaaccat ggctccttct ggtgattcac cttgcttatt
     1861 caaaacctcg gccacagggt atccggtttt ttataatgat ctgaaagaaa catctacaat
     1921 gggtattgca acaacaatta attggttcca agattctacc ttagtccaat gcatgtattg
     1981 gggatcacat gttggagtag atacaaccgg tttggttaat acagattcat ttggcactat
     2041 tatcaaagaa aaacctttat tgattaacta tggaaccggt gctttttggg gatctgatat
     2101 tacctttaat aacaggttaa ttcttgctgg atcagtttat tctggcagta cttttagtgt
     2161 atatctatac aaggttaact tcgatctcga atacgacagt atttatacca cccctttcac
     2221 ctacgacaac tgatgtccat attctattgt ttcggacact attcccctgg atgactgtga
     2281 agtgattgta tctattgatg atcccattga acatccggaa aagacgaggt taagggttta
     2341 tccggatccg gcaaaggaga aagtcacagt tgagatgccg gagtatctaa tcagaaaatc
     2401 agaaggtaat ggaatctctg ccacaacata ttaccatcag tggcctgagg tgcgaatggt
     2461 ggtgtttaat ctgttcgggc gaatgatatt cagtaaagaa atacaacaaa gtgaaaaaac
     2521 cgtgtcaata aatatttcaa cttggccaaa agggatgtat ttggtcagca tcgtattcat
     2581 gaatgatata gtagggagtg gtaaatttat tgtgaattag gtgtaatctt ccaaggaatg
     2641 agattctagt aagtgaaaac catgagacag caagccataa cttctagtat acagagtctt
     2701 gaaatactat gtcttaacag ttgggccctt cgtataaagt gcttagttgt gtattagtcc
     2761 ctataaatta ggacgagagt atagataaaa atctgtactt ttatgtctat gaaaaaaaat
     2821 tcaccaaaga agagaagctt caaattatta aggaagcctc ccagaacggg gttaacccaa
     2881 cctttgaaaa atatggagtt tatcaagcat catatattat caaataaaca taataggacg
     2941 tgtacccaat gagatagtgg tggaagaaat aaaaaaatat aaagaaggat actgacactg
     3001 attatggata tattaagatg acttattatt tgctcatact tggatatctt attaaccata
     3061 agaaggttta caggttaata atcaaaatcg attcctgagc ttgagggaag ttccttgtcc
     3121 tcatccgaaa tccctcgatg ggatagagga tgagaattta gaaaaagaaa tgtccggcgc
     3181 cgaaacattt ccacaaccat catcggtata acaatcgccg ttggttgttc cttgtcgacg
     3241 caaagttctt taactttttt taattttgta aaaaccttct atacgattgt tcgaacaata
     3301 ggggttatgc cctgctaaag atctggtgtc cattctgcaa tgttgtacaa agattctatc
     3361 agaatttttc tggattgatt ttcttagata ccacaaatat tctccatgtt tgatacatga
     3421 atttagcttt tagatctggt aaaattttct tgttaaattt gtcgccagac atttattata
     3481 caaattttgt aaattaaaat gaaaatctgc aaaagatgta ttttaaatga aaattatccg
     3541 ggtataacct ttaatgatga tagtatatgt tcattatgta tcagtaaaac ggattttata
     3601 cctaagggtg aagaaaaatt aatcaggatt tttgaaaaag ccagaaaaag aaataagaaa
     3661 tatgatgctt tggtaccttt gagtggaggc aaagacagta cttatattct acatttagcc
     3721 gtaaatattt ataatttaaa agtcattgca atgacttatg ataatggctt tatgagtgaa
     3781 ctatcgctcg acaatataaa aaatgcggtg gatataacaa aggttgacca tgttttttat
     3841 aaacccgatc aagaactaca gaagaagata tataggataa tgcttctaaa aactggtgat
     3901 atttgtggag catgcgatat tgcaaccagg gcatgtattt taaaagcttc gttggaatat
     3961 gatgtcccaa tagttttata cggtacatct cccttagaag ataattcttt tgttcccgat
     4021 tcgatagaag atattggacg ttttaaatat gttctaataa gttctggtga aattaatgac
     4081 aaagagatca aggattttac gatatatcca tggcttaatg acttaatatt gtcgttcaat
     4141 aaattagttg gaatctatcc cagagaagta aggcctttat tttacataaa aaatccatcc
     4201 gataaagaaa tgggtgaaat aatttcgaaa gaattaaaat ggagagacga tggtcgggaa
     4261 tattcacggc atttggactg tattgccgaa ccttttacga attacataag gaatagaata
     4321 tatggttatg aaagaagaat ttgtcaatac agtaatatgg tcagaaataa tgaaatttca
     4381 agagcacatg cactcgaact ctacgaagat gataaaattg atcagcctcc tgaaaattat
     4441 attgaaatac tgaattattt gggattaaac gagaaagatc tcgatcaaat tttatcgtat
     4501 aaacccttga tgtatgaaga ttatttatca agaatgaata ggatatatca gtggctggcc
     4561 aaatttaaaa gattcttaca ataaactgaa ccaatgaaga tcatctcaat cattgccgag
     4621 aactggaaag ccgatggagg agccgcattt ggcgttgttc ccaagacaat ttgggggaaa
     4681 ttatatcctg ctgatgacga taatctgcaa aagattacca gcaggtgctt actggttgac
     4741 acgggttcga gggtaatttt gttcgataca ggcatgggaa acaaacagga cgaaaagttt
     4801 ttccggtacc gctatcgttt tggagatgat aatttatttg aaaatctgaa atatgcaggt
     4861 tattcggccg atgacatcac cgatgcggtc tttacacatt tgcacttcga tcattgtgga
     4921 ggagcctcat gttggaatag cgaaggttcg aaggtagtac gtgtttttcc gaatgccacc
     4981 tatcatgttt cgaaagctca agcccaatgg gccttgtatc ccaataaaag agaagctgct
     5041 tcgtatctga aagaaaatct cgaacctatt ttcgaagctg gccctttgaa ccttatagaa
     5101 aagcccgggc cattcattca gcaaattgat ttgcgtttct ataatggaca taccagcggt
     5161 cagataatcc ctttcatcca tcttaacaac caaaccgtcg tttttgtgag cgattttatc
     5221 ccatcttcgg ctcatctgcc tttggtatat attcctgctt atgatatcca acctttgatt
     5281 acccttaccg aaaaggaaga attccttcac gaagccattg aaaagcagta tattttagtt
     5341 tttggtcatg attacttcaa cgaagctgcc accgtaatca tcaaaggaaa gggttttcag
     5401 gccgagaaaa taggtaaact atccgaattt atttgaaaat tcagttgaaa ataatttatc
     5461 ttattgaatt ttaaagtatt gctgaataaa cttttgaaag aaatagtaaa aaacaaaata
     5521 gtggtataaa tcattcatat acaatatttt acaaacaaac tttcaaaact gtaatttgtt
     5581 agtgcgaatt tatttttatt agcccgtagg taagagcaag gtcaagtact tctgaaaagt
     5641 cagagggctt agcgatgata ttgaggtcgg ggccgataaa aaacatggca ggtgtaacat
     5701 taattccata gtcgattgcc ggcggtgtat cccatccttt tccatctgca attacaggcc
     5761 atgtgtaatg attatttcgt accgaatctt caacggctcg taaattagta tcgatcacaa
     5821 cggtgattat ttcccacttt ttttgatgag gtcgagcata aagagaggct attttgggta
     5881 aaagtgccga gcaatggtta caccagctcc cccagaatat caacaataca tagttacctt
     5941 tatagttgct gagttttatt tcatgatccc tcaaatcttt tcccttgatt tcgggggcag
     6001 gcgagcctgg tgaaagacgc ttcgaaatct ctattcggga ggtaagatgg ggattggtat
     6061 ttatacaatt tccctgttcg taacgttcgg aaatgaattg caccacacgc tcgaaaccga
     6121 agttctgaaa gccatcgagc aaatattctg taatcatatc atacgcttct gcataaggct
     6181 gaagtatact catgattatt tttattcctt ctatcaatga gatttctaat ttctttttat
     6241 cgaagttggg atttgcatat aattccaaat attctatcac aagccttgga taagcattgg
     6301 tatagagaag acccttgtcg tcgaaagaaa ttttatcgaa aaaatgttgt ttcagaaaat
     6361 ttttcctttc ttcacctaag agattggggc taacgaaagg ttttcgtcga gtcatcaaat
     6421 atttaccagc gagattttcc tggttctcgg taatgatttt gtttaaggtt gcctcgtagt
     6481 cgatttgccg taaattgtat tcatcgataa tttcttgata gaaaggaagt tcatgaggat
     6541 actcatccaa catgttttcc aagagttcct tgattttctc gtatctgact tcgtatttgg
     6601 taaattttag gaagtcggta tttcccttcg aatctataaa attcaaactg tcctgcaagg
     6661 ccgatatatt cgtgtgaaag gtaaggcttt tgccatcgta aaaaaattga aaaccatcgt
     6721 tttgctgagt aagtatgcac atctgggaag gaggaatata tttgggaaca accagattaa
     6781 cttctccatt ttcgttgcac ctgagcgaat cgaatgctct tagattttca ccataataat
     6841 aagcaattcc caatttcata ccggcagcac cttctatctt tccgctaatt gatgtttggc
     6901 ctaagcatga gtaggagagg agagatatta cagaaatcaa gatgaatttc gatatatggt
     6961 ttttcataaa atcttgttgc aaatccataa aaatgttcgt aagccccaca attaacaaat
     7021 ttatgccgtt ttagtcaatt tattatccaa tttactacac ttattcatat tttatgaagt
     7081 gaataaacat ataatagaaa gtgaatcaat aagtagaata tatgaatgat gagttttggt
     7141 aattctctat ttggagttta gggcgaagat gagataagcg gttatctttg cacaaactgc
     7201 caaaatatgg acgaaagcga agtattgatt ttgagaagca agatggagaa tttaactgaa
     7261 gatgcggctt taaaggaagt tgcggagtgg tttccttcgc gagtatgttt tgccagcagc
     7321 ctgagtgccg aggatcaggt gattttgcat ttgattgcca ctcaaaatct tccgatacgt
     7381 atttttacgc tcgagacggg gcggcttttc gaagaaactt atgatttact cgaaaaatcc
     7441 cgtttaaggt acaaaatcaa tatcgaagta attttccctg attataagcg agtggagaag
     7501 ttggtgaatg aaaaggggat taatcttttc tattatagtg ttgaaaaccg taaaatgtgc
     7561 tgtagcgttc gaaaggttga acctttaagg cgtgctctcg aaggaatgaa gttatggatt
     7621 accgggctca ggagacaaca gtcgcaaaca cgagaacaca cacagttatt ggaatgggat
     7681 gaggtaaatg gtttgttaaa attcaatcct ctcatagcat ggactgacga aaagttatgg
     7741 aatttcattc atgatcatca gattccttat aatcccttgc acgataaggg atatccgagc
     7801 ataggttgtg aaccttgtac tcgtgccata caacccggtg aagatgtcag agctgggcgc
     7861 tggtggtggg aagctcaagg aggacgcgaa tgtggaattc accgaagtta aaacatgttg
     7921 caaccaatat tttttaacgg agggtcaagc actaagaaat taaaaataaa gaagctgatt
     7981 ttaattttaa atggcaatat tttcagctaa gaattttttc aaattcatta tgagtcccta
     8041 taaagattat tattatatta tgtcggctct tgtgctacga gaatttttat atcaggttta
     8101 aatctcatag agaattcaat gacccacaat attctgaata tcctactgaa aaaaatccga
     8161 tatactggat ctgatcacta aaacatcgtc taacgaaaac agcattgcct tgaattattt
     8221 ctgtggctcg agaaccactt caaaatcagc ccatattcat catcgaaaat gggttggcat
     8281 cgataaagtc agcacctgac ataaaggcta ctattaaaaa aatgagctcc atacaatcag
     8341 acttttttta ccgccagcca catttgaatt gatagatttg agccagattc tttctacaaa
     8401 tactgatata gaatcttcta ttcagtaatc aaattttctc attcaaattt gatagtgagg
     8461 tattgttatt gcaaggttat acaaggcgat tattattgtt tatttcccct taaaaagttg
     8521 cattaattac cttgaactta caatttttga gttcgttttt gtgccacagt attaatctta
     8581 gaatgaatta tttgaataat aaataagaaa atttagaaat gaaaaagacc cggaactaat
     8641 gccccgggtc tttaacaaaa taaagcaaaa tgaacaaaat aataaataaa atggacttac
     8701 tgagatagtt tgaggttaat catagcttcg atgactttag cggtactatc ctgtccattt
     8761 tgagaagcta cctgacttaa ccgctgcaac agataggcat tttctgacat ctcttcttcg
     8821 tattgagatg aatattttgg ttcgagcgaa aggtagaaat tgagatcctg agaaaagata
     8881 ttagccaact gcagtgccat gttgttcgct ttttcggtag cttttgctgc gtaatataaa
     8941 tcaacatatg agagcatata tttatcgggg aatatttttg aggttgggaa atattgtaac
     9001 gacaaatcga gtgctttctc tgcctcttcg tttttgcctt tatttagcat agactgggca
     9061 acacgcatat agttttgtct tggtattgca gcgttgcgaa aactttccct gtcaaccgta
     9121 accctgggat cattgagatt tccccagtgt ttaatgcggt ttacaagaat atcatagcat
     9181 gtatcggccg acacacctcc caatcctcta atatagtcgg tggctttaac aggcaagaat
     9241 ttataaacca ttccctcaag atgactgtac tcatccaaat taatagctcc tccaattcct
     9301 gaaggactcg ttaaatatat aggtcttttc cagtcgttgg tggctaagat atcgagcaac
     9361 attaaatcat ttttaaagat ataatttctg cgaatagtga agtcgagata gggtaaaact
     9421 ctatttttca tttcgtttgg gatgaggcca cttgctttta ctgcggctgt atccacaggg
     9481 atacgaatgg tcttggaagg tatgaagcta agcctttggc catcgccaaa atcacgcttg
     9541 tttctttcat cggcaataaa tctcatgatg tccctcagca gaatgggttg tcccgatttg
     9601 ttcagatcaa ccaccactac gtaatcgtta ttgcccttat catattgttc gggtttcagg
     9661 gtaaatggaa ggggttcaga gtcatagact tttcgggcca tttgatggat gtaccagtcg
     9721 cctgaagcca gcatgtagtt tactactctt acgtcggtgc gatgattttc tacctcttgg
     9781 gcataccaca aggggaaagt atcattatcg ccgaaggtaa aaagcaggct ttgaggctgt
     9841 gttgaatcga gataagcaaa ggcataatcg cgggctgcag ttttatgtga acggttgtgg
     9901 tcgtcccatc catctttggc catgacatag ggaacgggca atgcaatcaa aatagctaag
     9961 atggctgctg caacgggttt gatgtatttc ccgagccaat cgtttagggc aagtactcct
    10021 aaccccaccc agatggtaaa ggcataaaag gagcccgcat agctgtaatc tctttcacgg
    10081 ggttggaaag gagtttggtt caaatagata acgatagcta ttcccgtcat gaagaagagc
    10141 aaagccacca caaaactatc gcgtggatgt ttcttaatat gatagaataa accaatgagg
    10201 cccaaaataa gtggcagaaa ataaaaacga ttgtatgccg gattttgttt gcggtattcg
    10261 ggataatttt cctgattgcc taatctggcc tgatcgataa acttaatccc acttatccag
    10321 ttaccgtctt ctatccctcc atgaccttca atgtcgtttt gcctgc
//
LOCUS       DAOORU010000083        10344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8045_length_10344_cov_5.397609, whole genome shotgun sequence.
ACCESSION   DAOORU010000083 DAOORU010000000
VERSION     DAOORU010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10344
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8045_length_10344_cov_5.397609"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1096)
                     /locus_tag="PLF20_06400"
     CDS             complement(104..1096)
                     /locus_tag="PLF20_06400"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOX80668.1"
                     /translation="MKNLLIANGLLFIFMISACNRAPSPSSRSSVDDAGTDGTLIIFH
                     AGSVTRPVRVIADSFEKKHPRVKILHEICGSRECAKKVTELEKPCDILISSDIDVIES
                     MLYPEYVEGWIGFATNEMVIAFTETSRYHRQISAQNWPDILLKKDVNYGRSDPDSDPC
                     GYRTLFTFQLAASYYHRPELAKILASKNQKYIRPKEMDLLALLQSGSLDYIFIYRSVA
                     LQHHLKFISLPPEINLSNPLLASEYAKAVVSVSGKKPGEKQLVYGSPMVYGITLIRNA
                     PHKKLALLYLDEFLSPKEGLAVFEANGQPALDPLICYPLDSLSASQPCNGQFFR"
     gene            complement(1109..2818)
                     /locus_tag="PLF20_06405"
     CDS             complement(1109..2818)
                     /locus_tag="PLF20_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80669.1"
                     /translation="MISKKLFLKLSTTIGVLLWLPIVEKSFGQHPQIEEVTIIGSFTP
                     HISDANKILRNPMLRDTSIILKPMEYQIISRPLHFSFPLYSIEAQKVQAESDAMYLKN
                     YLRLGMGSLLNPYADFYANIPQSKRTAFGIHYRHLSAQADLEDYGESSFSDNLAEIYG
                     KTIGKNLAWNGGINFQRNMLHWYGFNPAKFSLPANSDTSITKQVYTRLCGNIELKKNV
                     SNHDPFYFDSRLDALWMEDKYGTSELNLSYFGNIQFTNSILSARSNDNIGLGLEASFW
                     NNQWDIYPDDRTGLISFHPYFSTQIDEYELYAGLNTSITVDSGDTKVHVFPELKGSLT
                     IVKEVLKAYIGIKGDICRNNLNQFSTINPFISSYVPFNFRKNSFEFYGGLSSNLGRYV
                     GLSASISTSNFKNQAFFINDTTVPLYNQFTAIYDSGSVLKVNAEISFHKQENIIFVLG
                     LNYSSYSLENLHKPWHVPEFMVYANGRFVIGQKFIVKPIIYFQSMTYANIFEKQNGKW
                     KESEPLKLDPFLDLNIGLEYRINPKFSTFTNFNNITGGNYQRWYNYPVHAFNVMAGIT
                     YSF"
     gene            complement(2847..5849)
                     /locus_tag="PLF20_06410"
     CDS             complement(2847..5849)
                     /locus_tag="PLF20_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX80670.1"
                     /translation="MKKLVFIACGVCLSIFFIPIKAQKSYSLIDDEIQWQTAIELFER
                     EKYGAAQHIFEQISKNTNAEKKMLANYYSAVCSFKNKNPDAGQQLMNFIQKYPTSDKI
                     NQVKFYRACFLFNNKDFKSADQLFTETDTYELPPSHLPEFYFKAGYCRFLVGDMERAK
                     NYFYEIKDGNHIYADQASYYYGHILYKQGKYENALQEFDKLINHPDYKSVIPYYIAQI
                     YYRQGKYEELLQTASPLLENTLGRQKADIARMIGDAYLQLENYSKAYEYLLMHEQLTK
                     AKPDRAQAYSLAYAAYKSNLFEEAISYFQIAAQGKDSLSQNAWYHLGDCYLKTGQKDF
                     AQNAFYTAYQLSYNQTSREDALFNYAKLSYELASDPYNESISALKKYLTEFPESHRTD
                     EANQYLINLALISKNYKQALEAIENIKFKDIWIKTAQQRILYYLGIESFNENKYQEAI
                     HYLQEAAGMDFDKTIRLQALYWTAEAIYRTGDYVSALKAYHKFLLSAGAVDIPEYTSV
                     HYNIGYAQFKLSQYDEASVSFRKFLSTYQGNDKAMINDAYLRIGDCFFIGRNYTEAIK
                     YYQKAYNNGSTEADYALYQIALSQGVQKQFQEKIATLKNLISKYPRSPYNPAAYYELA
                     LTSLLINKNNDALGYLDQIINKYPKSNYVSKSMLKKGMIYYNDNQNEKALVELKAVVN
                     KYPGTSDAQEALVAISNIYVEMNRVDEYLSYLKTVPNAELLMSGQDSLIYVAAENQYQ
                     KGDCKSATEGFYQYVRNNPQGRFSANAWFYIGECARRTGNSSGALQAYENVTLRPGNP
                     FMDRASSYAAQLRFMQKDYTRALEHYQRLESVAADKNSLTEAFAGQMRCFYQLGEMNA
                     VIDAANQLMKSEKVTHNLLVEAHLYMGRAAFHLNNIPLAEKEFGIITRLTQSEAAAEA
                     QYHLSLITFNRNDLSNAEKMTFQLINNYPSADYWRVKGFILLSDIYAANGNIFQARQT
                     LQSIIDNYKGEDLRKEAQNRLNNLPPVQ"
     gene            complement(5941..6414)
                     /locus_tag="PLF20_06415"
     CDS             complement(5941..6414)
                     /locus_tag="PLF20_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008629484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOX80671.1"
                     /translation="MKINLIAIGKTDEAYVEDGCQIYEKRIMHYLPYARYEIPSTKIN
                     YKYDKNRQLEAEASLLEKNLEPEAFVVLLDEHGKEFTSVGFAQFLQNRMNAGIKNLTF
                     VIGGPYGFHQRIKQRANEIMSLSLLTFPHQLVRLIFVEQLYRALTILNHEPYNHS"
     gene            6593..7000
                     /locus_tag="PLF20_06420"
     CDS             6593..7000
                     /locus_tag="PLF20_06420"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HOX80672.1"
                     /translation="MKILLQKEFLLFLAIFSGFSASVCAQNNQAVIVKAISDGDAHEL
                     VKYFHTMVNLAIPGSEGVYNRNQSEALLQKFFSQNEVLSFKVKHEGRSNEGTRFLIGK
                     MNCPTKIYNLYYLMKEMEGKYWIIQFQLEPFEE"
     gene            7025..7861
                     /gene="nadC"
                     /locus_tag="PLF20_06425"
     CDS             7025..7861
                     /gene="nadC"
                     /locus_tag="PLF20_06425"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOX80673.1"
                     /translation="MNVEEIIELALREDIGEGDHTTLATVSRRQRAHARLLIKGKGVI
                     CGIDIARRIFQRLDSSMEFTEILHDGAEVEAGEIGFTLYGNARAILMGERTALNFMQR
                     LSGIATQTCQLVKLTEGFPVRLLDTRKTTPLLRQLEKYAVRVGGGVNHRFGLFDMILI
                     KDNHVDYAGGIAQAIRAVNQYLKENHLDLKVEIEVRNLTELNHVLDEGHIHRIMLDNF
                     TPDELKMAIKIIGGRFETEASGGITLQNIREYAATGVDYISVGALTHHIKSLDLSLKA
                     YI"
     gene            7862..8836
                     /locus_tag="PLF20_06430"
     CDS             7862..8836
                     /locus_tag="PLF20_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOX80674.1"
                     /translation="MKNRFISNILFEDTWIGRQLDKLSRRGLCIIHWSKKIVVPGFEG
                     MPLYDVMTFFLKSLTKGNISAKASGIAYSFLAAIFPAIIMLFTIIPFIPIENFQPMLM
                     GLLEDFLPDEIWRVVNKTVEDIITRPRGGLLSVGFIMALYFCSNGITGLTNAFNSSVH
                     AFKTRSWFKQKAINIFLILLYTILLIVAIALISLGSLTFSFLNEHHIISNIFTYYLLQ
                     IGRWIILIATVYFGISFLYYFAPDRRNKFKFFSAGGTLSTFLILLTSWGFSFYIQNFS
                     RYNAVYGSIGTVLILMFYLWLNSMILLIGFELNASIRQARMNKGMRDS"
     gene            8878..>10344
                     /locus_tag="PLF20_06435"
     CDS             8878..>10344
                     /locus_tag="PLF20_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80675.1"
                     /translation="MKRFRHQLLLLAFVLVGNENILFAQLEANNGYFGNYAGVSFASG
                     ETVALLDGALNTSEGVATISNSSGLLLFYIDGQTIWNRNHQIMSNGTGLWGHSSSTQS
                     GVVVKKPGNNTLYYVFTMDQVATGGIHGVSYSIVDMNLEGGLGAVTIKNIEIVSNSNC
                     TEKITAVKHTNGMDIWVITHGWNNNQFLAFLVTNSGINTTPIISNTGQIHTGINRHRA
                     GYLKSSPNGTYLALAVYDYDLSVMIEMVKFNTLTGIVTTDSKCFYDFIPSAYGIEFSP
                     DNTKLYASGARDNGKIFQYDLNLPNGSSIVNGKTEIASNGNYAAMQVANNGRIYISRY
                     NYTFLCTINNPNNAGLSCGYVENNVSLEGRTATFGLPTFIQSFFVSVQAYSNSPLCVG
                     ATLYLYCIAAGAVSFEWIGSAGFSSNLQNPVISNVQTLNSGIYTVTAYSASGTYNSAQ
                     VIVNVNPNPQISLGPDTTICSNTSIIITPGNGYQSYLWN"
BASE COUNT         3082 a         1881 c         2113 g         3268 t
ORIGIN      
        1 attattttat cttttttgaa attatattac ctaattttgg agggtttagg tttaaacatt
       61 tggggaaaaa taatttacag tcgatagata aaaaaagaaa aattcagcga aaaaattgtc
      121 cattgcatgg ttggcttgcc gaaaggctat cgaggggata acaaataagt gggtctaatg
      181 caggctgacc attggcttcg aaaacagcca agccttcttt gggtgataag aattcatcga
      241 gatacaacag ggcaagtttc ttatggggtg catttctaat aagtgtgatg ccgtaaacca
      301 taggactgcc atatactaat tgtttttctc ctggtttttt accactgaca ctcacgactg
      361 cttttgcata ctcagaagcc aacaaaggat tggaaagatt gatttcaggt ggtagcgaaa
      421 tgaatttcaa atgatgttga agggcgacag aacgataaat gaatatataa tcaaggctgc
      481 ccgactgcaa taaggccaaa agatccattt cttttggccg gatatatttc tgattcttcg
      541 aagccagaat tttggctagc tcagggcggt ggtaatatga tgccgcaagc tgaaaggtga
      601 acaaagttcg atacccgcaa gggtcggaat caggatcgga acggccgtaa ttcacgtctt
      661 ttttcaataa tatatctggc cagttttgtg cggagatttg tcgatggtat ctcgaggttt
      721 cagtgaaagc tatgaccatt tcgttggtag cgaagccaat ccaaccttct acgtattcag
      781 ggtaaagcat gctttcaatc acgtcgatat ccgagctaat caaaatatca cagggttttt
      841 cgagttcggt tacctttttt gcacactccc ggctaccgca tatttcgtga aggattttca
      901 ccctcggatg ttttttttcg aaactatctg cgataactct caccggacgg gtaacgctcc
      961 cggcatgaaa aattatcagt gtgccatcgg tccccgcatc atccacactt gatcgactgg
     1021 aaggtgaagg agctctatta caggctgata tcataaatat aaataaaagc ccgtttgcaa
     1081 tcaatagatt tttcataaac aaacccggtt aaaaagaata agtaattccc gccatcacat
     1141 tgaaggcatg tacaggataa ttgtaccagc gttgataatt cccacccgta atgttattga
     1201 aattggtgaa agtcgaaaac ttaggattaa tgcgatactc gagccctata ttcaagtcga
     1261 ggaatggatc gagtttcagg ggttcagatt ctttccattt cccattttgc ttttcgaaaa
     1321 tattggcata ggtcatactc tggaagtata ttatgggttt cacaataaat ttctgcccaa
     1381 ttacaaaacg tccatttgca tagaccataa attctggaac atgccacggt ttatgcagat
     1441 tttccaacga ataggaggaa taattgagtc caagaacaaa gatgatgttt tcctgtttat
     1501 gaaatgatat ttctgcattt accttgagaa cgcttccgct gtcgtaaatg gcagtgaatt
     1561 gattatataa tgggacagtg gtatcgttga tgaaaaacgc ctgattttta aaattagaag
     1621 tgcttatgct ggcactcaac ccaacataac gtccaaggtt ggaactcagc ccaccataaa
     1681 actcgaagga atttttcctg aaattaaaag gaacatagct cgaaatgaaa ggattgatcg
     1741 tactaaactg gttcaaattg tttcggcaga tatcgccctt aatccctata taagctttca
     1801 gaacttcctt gacgatggtc aaactacctt tcagctcagg gaaaacatgg actttagtat
     1861 ccccactatc cacagttatt gatgtattta aacctgcata caattcatat tcatcaattt
     1921 gcgtactgaa ataaggatgg aaactgatga ggcctgttcg atcatcggga taaatatccc
     1981 attgattgtt ccaaaagctt gcctccagcc ctagcccgat gttgtcgttg cttcgggcag
     2041 aaagaatgga attggtaaat tgaatattgc caaaatatga gagattcagt tcggaagtgc
     2101 catatttatc ttccatccac aaagcatcga gcctcgaatc aaaatagaaa gggtcgtggt
     2161 tagaaacatt tttctttagt tcaatatttc cacatagtcg tgtatatact tgttttgtaa
     2221 tacttgtatc tgaatttgcc ggaagtgaaa atttggccgg attaaatcca taccagtgaa
     2281 gcatgttcct ttgaaaattt attcctccat tccatgcaag atttttccct atggtttttc
     2341 catagatttc ggccagatta tcgctaaatg acgattcacc ataatcttct aaatcggctt
     2401 gggcggaaag atgcctgtaa tgaattccga aggcagtacg cttggattgc ggaatattgg
     2461 cataaaaatc ggcataaggg ttgaggaggc ttcccatacc taatcgaaga taatttttta
     2521 gatacattgc atcgctttcg gcttgaactt tttgcgcctc gatagaataa agcggaaagg
     2581 aaaaatgcaa aggcctcgag ataatttgat attccatggg tttgagaatt atactcgtat
     2641 ctcttaacat gggatttctc aaaatcttat tggcatcgga gatatgggga gtaaaacttc
     2701 caatgatggt tacttcttca atctgaggat gctggccaaa gcttttttcg acgatgggaa
     2761 gccacaaaag aaccccaatg gtcgtactta atttcaagaa aagtttttta cttatcatat
     2821 tttcaatggg tttgtcgaaa tgagaattat tgaacagggg ggagattatt caagcggttt
     2881 tgagcctctt tgcgcaagtc ttctccctta taattatcga tgatgctttg cagagtttgc
     2941 ctagcctgaa agatattacc atttgcagca taaatatctg aaaggagaat aaatcccttc
     3001 actctccaat aatcagcgga aggataatta ttgataagct gaaaagtcat tttttctgca
     3061 ttggataaat cgttgcggtt gaatgttatc aaggaaagat gatattgagc ttctgctgcg
     3121 gcttccgact gtgtaagccg tgttatgata ccaaattctt tttcggccag aggaatatta
     3181 ttcagatgaa aagccgcacg tcccatatat aaatgagctt caaccagaag gttgtgggtt
     3241 actttttccg atttcatcaa ttgattagcg gcatctatta cggcgttcat ttcaccaagc
     3301 tgataaaaac agcgcatttg gccagcaaag gcttcggtaa gagaattctt gtcggccgca
     3361 acagattcta gacgttggta atgttcgagg gctcgagtat aatctttttg catgaagcga
     3421 agctgtgccg cataactcga agcacgatcc atgaagggat tgcccggcct taaagttaca
     3481 ttttcataag cttgaagggc cccagaacta ttgcctgtcc ttcgggcaca ttccccaatg
     3541 taaaaccacg cgttcgccga aaatcttccc tgaggattat ttcttacata ttgataaaat
     3601 ccttccgtag ccgatttaca atcgcctttc tgatattgat tttcggccgc tacataaatc
     3661 agggagtctt gtcccgacat taaaagttca gcattgggca cagttttcag atacgacagg
     3721 tattcatcta cccgattcat ttctacataa atattactga tggccaccag tgcttcctga
     3781 gcatcagacg taccgggata tttattgact actgccttta attctaccag ggctttttca
     3841 ttctgattgt cgttataata aatcatccct ttctttagca tggatttgct cacataattg
     3901 ctcttaggat atttattgat aatctggtcg aggtaaccta aagcatcatt gttcttgttg
     3961 ataagaagag aggtgagggc taattcgtag taagccgctg gattgtatgg agagcgcgga
     4021 tatttggaga ttaaattttt cagcgtagca attttttcct gaaactgttt ttgaacacct
     4081 tgcgaaagag caatctgata cagggcataa tcggcttcag tacttccatt gttataagct
     4141 ttctgataat atttaatggc ttcggtataa tttcgaccaa taaaaaagca atccccaatt
     4201 ctcaggtaag catcattgat catggcttta tcgttgcctt gataagtaga aaggaatttt
     4261 ctaaacgaga ccgaagcctc atcgtattga ctcaatttaa actgtgcata cccgatgtta
     4321 tagtgcacgg aggtgtattc aggaatatcg acggcgcccg cgcttagtaa aaatttatga
     4381 taagccttga gagccgaaac ataatcgcct gttcgataaa tggcttcggc agtccaatat
     4441 agagcctgaa gccgtatggt tttgtcgaaa tccataccag cagcttcttg cagataatga
     4501 atggcttcct gatacttatt ttcattgaac gattctatgc caagataata aaggatgcgt
     4561 tgctgagcag ttttaatcca tatgtcttta aacttaatgt tttcgatggc ttcgagcgct
     4621 tgcttgtaat ttttgctgat gagagcaagg tttatgagat attgattggc ctcatcggtg
     4681 cgatgcgatt cggggaactc ggtcagatat ttttttaatg ccgagatgga ttcattatat
     4741 ggatcggaag ccaattcata agaaagttta gcataattga acagagcatc ttcacgcgat
     4801 gtttggttgt acgataactg ataagctgta tagaatgcat tctgagcaaa atctttctgt
     4861 ccggtcttca agtaacaatc gcccaagtga taccatgcat tttgagatag ggagtccttt
     4921 ccctgggcag caatctgaaa ataggaaatg gcttcctcga aaagattaga tttgtaggct
     4981 gcataagcca aactataagc ctgtgctctg tcaggcttgg ctttggtaag ctgctcgtgc
     5041 atcaacagat attcataggc tttggaataa ttctcaagtt gcaagtaagc atctccaatc
     5101 attcgggcaa tgtcggcttt ctgcctaccc aaggtgtttt cgagcaaagg agaagcggtt
     5161 tgcaaaagct cttcatattt tccttgacga tagtatattt gggcaatgta ataaggtata
     5221 accgatttat aatcaggatg gtttattaat ttatcgaact cttgcagggc attttcatat
     5281 ttcccctgtt tatatagtat gtggccatag taatatgaag cttgatcggc atagatatga
     5341 ttcccgtcct taatctcata aaaatagttt ttggcacgtt ccatatctcc caccaaaaac
     5401 ctgcaatatc cagctttgaa atagaattcg ggcagatgcg aagggggtag ctcgtaggta
     5461 tctgtttccg taaataactg atcagccgac ttgaagtcct tattattgaa aagaaagcaa
     5521 gctcgataaa atttcacttg gtttatttta tcgctcgtag gatatttttg gatgaaattc
     5581 atcaactgct ggccagcatc agggtttttg ttcttaaaag aacaaaccgc cgaataatag
     5641 ttggcgagca tcttcttctc agcattggta tttttggaaa tttgctcgaa aatatgttgt
     5701 gctgctccat acttttctct ttcgaaaagc tcgatggccg tttgccattg aatctcatca
     5761 tcaatcaaag aatatgattt ttgggcttta attggaataa aaaatattga aaggcacacc
     5821 ccgcaagcaa taaaaacaag ttttttcatg gaattaagtt cttgagatta attaaaacca
     5881 gaataaatta gatttacata aagaaacgga ttcgaaggtt tcatatttta tgattttcgt
     5941 tcaggaatga ttataaggct catgattcaa tatggttaag gctctgtata attgttccac
     6001 aaagataagg cggaccagtt gatgcgggaa ggttaagagg gaaaggctca ttatttcgtt
     6061 ggctctttgt tttatgcgtt ggtgaaaacc ataaggaccg ccaatcacaa aagttagatt
     6121 ctttatccct gcgttcattc tgttttgtag aaattgagca aatcccacag aagtaaattc
     6181 ctttccatgt tcatcgagta agaccacaaa agcttcaggt tcgagatttt tttctaaaag
     6241 cgaggcttct gcttcgagct ggcgattttt gtcatactta taatttattt ttgttgaagg
     6301 tatctcataa cgagcataag gaagataatg cataatacgt ttttcgtata tttggcaacc
     6361 atcttccaca taggcctcat cagttttccc gatcgctatt aagttaatct tcataagtaa
     6421 aatttagagc aagtgcgaaa ttatgatttt tcgaccttat tgtattgaat tcctcatggt
     6481 aatcatattt taccataaat ttgctcattt tcgcaaccaa tgcttatggc acttttattg
     6541 taatataaac cgtttcagaa tttctttcat tattcattaa ttcgaaaggg gcatgaagat
     6601 attattacaa aaagaatttt tgctgtttct ggcgattttt tcaggctttt cggcttctgt
     6661 ttgtgcccag aataaccagg ctgtgattgt aaaagctatt tcggatggcg atgcacatga
     6721 attggtaaag tattttcata ctatggtcaa tttagccatt cccggaagcg aaggtgtata
     6781 taaccgtaat cagtcggaag cacttttgca gaaattcttc agtcagaatg aagtattatc
     6841 attcaaggta aagcacgaag gacgatcgaa cgaaggaaca cgttttctta ttggcaaaat
     6901 gaattgccct actaaaattt ataatctgta ttatttaatg aaggagatgg aggggaaata
     6961 ctggattatc cagttccaat tagaaccatt cgaagaatga ttctcaagaa acattgaaat
     7021 taaaatgaat gtagaagaga ttattgaatt agcccttcga gaagatatag gtgaaggtga
     7081 tcataccacc cttgcaaccg tatctcgacg acaaagagcg catgctcgat tactgataaa
     7141 gggaaaggga gtcatctgcg ggatagatat tgcccgaaga atatttcagc gcttggattc
     7201 ctccatggaa tttactgaaa ttttgcacga cggagccgaa gtagaagccg gagaaattgg
     7261 ttttacgctt tatggcaatg ccagggctat acttatgggt gaaaggactg ctcttaattt
     7321 tatgcaacga ttgagcggca tagccactca aacctgtcaa ctggtaaaac ttactgaagg
     7381 cttcccggtt cgattgctcg atacgcgcaa aactacccca ttacttcgtc aactcgaaaa
     7441 atatgctgta cgggttggag gaggagtcaa tcatcgtttt ggacttttcg acatgatcct
     7501 tatcaaggac aaccatgtgg attatgccgg aggaattgca caggctatca gagcagtgaa
     7561 tcaatatctc aaagaaaacc atcttgattt gaaagtggaa atagaagttc gcaacctaac
     7621 tgaattgaac catgtacttg acgaagggca tattcaccgc attatgctcg ataattttac
     7681 acctgatgaa ttgaaaatgg ccataaaaat aatcggaggc cgttttgaaa cagaagccag
     7741 tggaggcatt acgttgcaga atataagaga atatgctgcc acaggggttg attacatctc
     7801 ggtaggtgct cttacccatc atattaagag cctcgatcta agtttgaagg cttatatata
     7861 agtgaagaac cgctttatat cgaatattct tttcgaagat acttggattg gccggcaatt
     7921 ggataagctg agccgaaggg ggctttgtat cattcattgg tcgaagaaaa tagttgtacc
     7981 tggtttcgaa ggaatgcctc tatacgatgt aatgacattc ttcctgaaat cgctaacgaa
     8041 ggggaatatc agtgccaagg cttcgggtat agcctatagt tttttagctg ctatctttcc
     8101 tgcaatcata atgctattta ctatcattcc ttttatccct atcgagaatt ttcagcctat
     8161 gctgatggga ttgctcgaag atttcttacc cgatgaaatc tggcgagtgg ttaataaaac
     8221 agttgaagat atcatcacac gaccacgagg tggtttgctc tccgtagggt ttatcatggc
     8281 cttgtatttt tgctcgaatg gcataaccgg tcttaccaat gcttttaatt cgagtgtgca
     8341 tgcttttaaa acacgttctt ggttcaaaca aaaagccata aatatattcc tcatacttct
     8401 atataccata ttgctgatcg ttgccattgc cctaatcagt ttgggaagtc ttacctttag
     8461 ttttttgaat gaacaccata tcattagcaa tatctttact tattatctct tacaaatagg
     8521 gaggtggatt attttgatag ctactgttta ttttgggata agctttctct attatttcgc
     8581 acccgacagg agaaacaaat tcaaattttt ttcggccgga ggaacattgt ctactttcct
     8641 tatcttatta acttcttggg gttttagctt ttatattcag aatttttcac gctataatgc
     8701 ggtttatggc agtattggta ctgtgcttat tttaatgttt tatctttggt taaattctat
     8761 gattttattg ataggtttcg aattaaatgc cagtattaga caagccagaa tgaataaggg
     8821 aatgagagat tcataaatat tattaccttt accctatctt tatacgaaat tttttaaatg
     8881 aaacgattta ggcatcaact gttgctattg gcgtttgttt tagtgggtaa cgaaaatatt
     8941 ttgtttgccc agcttgaggc caataacggg tattttggca attatgctgg cgtgagcttt
     9001 gccagtggcg aaaccgtagc actgttagac ggggcgctga atacaagtga gggtgtagca
     9061 acgattagca attcatcagg tttacttctt ttttatatcg atggtcaaac tatctggaat
     9121 cgaaatcatc aaataatgtc gaatggtaca gggttatggg gacattcttc tagtactcaa
     9181 tcgggtgtcg tagtcaaaaa acctgggaat aatacccttt attatgtgtt tacaatggat
     9241 caagttgcaa caggaggcat tcatggggta agctattcta ttgtggatat gaatttggaa
     9301 ggagggttag gagcagtaac tattaaaaat attgaaattg tttctaattc aaattgtact
     9361 gaaaaaataa ctgctgtaaa gcataccaat ggcatggata tatgggtaat aacacatggt
     9421 tggaataaca atcaattttt agcgttcttg gttacaaaca gcggaataaa tacaacccca
     9481 ataatatcaa atacaggcca aattcataca gggataaata gacatagagc gggatattta
     9541 aaatcatcac caaatggaac atatctggca cttgcggtat atgattatga tctttctgtg
     9601 atgattgaaa tggttaagtt taatacatta acgggaattg taactacaga ttcaaaatgt
     9661 ttttatgact ttataccttc tgcttacggt atagagtttt ctcctgacaa cactaagtta
     9721 tacgcatcag gcgctagaga taatgggaaa atttttcaat acgatttgaa tttacccaat
     9781 ggcagttcta tcgtaaatgg aaagacagaa attgcctcta acggaaatta tgctgctatg
     9841 caggtggcta ataatggcag aatatacatc tcacgttata actatacatt tttatgtaca
     9901 attaataatc ctaacaatgc cggattgagt tgtgggtatg tggaaaataa tgtctccctc
     9961 gaaggcagaa cggcaacttt tgggttacct acttttattc aaagtttttt cgtatcagta
    10021 caagcctatt caaatagtcc cctttgcgtg ggggcaactc tttatctata ttgtatagcg
    10081 gctggtgcag tttcttttga atggataggc tctgcagggt ttagctcaaa tcttcagaat
    10141 cctgtaatta gtaatgtgca aaccttaaat tctggtatat atactgttac agcttattca
    10201 gccagtggta cttataattc agcacaagta atagttaatg ttaatcccaa cccacagata
    10261 tcattaggac ctgatacgac gatttgttct aatacttcaa tcataattac tcctggtaat
    10321 ggatatcaat catatttatg gaat
//
LOCUS       DAOORU010000084        10251 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8156_length_10251_cov_6.107493, whole genome shotgun sequence.
ACCESSION   DAOORU010000084 DAOORU010000000
VERSION     DAOORU010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8156_length_10251_cov_6.107493"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLF20_06440"
     CDS             <1..482
                     /locus_tag="PLF20_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80676.1"
                     /translation="MIIVSHDRDFLQGLTHRVIEFKQHHINEYLGDIYDFLSARQIDT
                     LRELDTENKIIQNNQILQQSQNKIEYEKRKAHERKRRKIISEIEKCEEAVFQTDNELA
                     EMEHKLANPAQYQDTINSKDFFNRYDYLKKKSERLMTEWEDLHLMLEELDHSDLTDS"
     gene            complement(462..1448)
                     /locus_tag="PLF20_06445"
     CDS             complement(462..1448)
                     /locus_tag="PLF20_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOX80677.1"
                     /translation="MDTEIKSIEQIHELINKEIESLPFSGYPSNLYDPIAYMMHQNGK
                     RLRPALALIACNMFGGDLKKILRPAIGIEIFHNFTLVHDDIMDQSPIRRNHETVYRKW
                     GTNVAILVGDTMFALAYEFLIQTDEGFLGEILRLFNKTAIQVCEGQQLDIEFENRTDV
                     SIDEYIEMIREKTAVLLGTSLMTGAIVARTPKQQAEQLYQMGIKMGLAFQLMDDILDC
                     YAQQDKFGKSIGNDIMTNKRTYLFLQAYQKANPSQRIELDECFGNNTMPADEKISRVK
                     HIYDDLKVGDDGYALADGYFSDALRLLDELPVEDNRKIGLKTLIERLAVRQV"
     gene            complement(1467..2333)
                     /gene="rfbA"
                     /locus_tag="PLF20_06450"
     CDS             complement(1467..2333)
                     /gene="rfbA"
                     /locus_tag="PLF20_06450"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOX80678.1"
                     /translation="MKGIILAGGSGTRLHPLTLALSKQLMPVYDKPMIYYPLSVLMMA
                     DIREILIISTPNDLPNFERLLGDGSQIGCRFSYAVQEIPNGLAQAFVIGEKFIESDKV
                     CLILGDNIFYGRGLQELLLASNDPEGGIVFAYHVSDPERYGVVEFDENFKAISIEEKP
                     KVPRSNYAVPGLYFYDNSVVKIAKNIKPSARGEYEITDVNRHYLVKGKLKVGLLSRGI
                     AWLDTGTFESLTQASQFVEVIQNRQGLLIGSIEEVAYRRGFITKDQLLELAEPLLKSG
                     YGRYLQRIAETE"
     gene            complement(2384..3604)
                     /locus_tag="PLF20_06455"
     CDS             complement(2384..3604)
                     /locus_tag="PLF20_06455"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOX80679.1"
                     /translation="MKESIVILTGGGPAPGVNTVIGTVSKVFLKSGYHVIGIHEGYKT
                     LFSDEPNITDIEFRLADNIYKTGGSFLQMSRYKPKEEEFNTRFFTKHNVKLLVTIGGD
                     DTASTANKISMFLQENRLDVKNIHVPKTIDNDLPLPEGQTTFGYESAKEEGVRLATTV
                     YEDARTSGNWFIVSAMGREAGHLAFGIGASCHYPMIIIPEMFNKTEITIDKILRLIIS
                     AMIKRKLLGISYGGAIISEGVFHFLSDEEILSTGINFTFDEHGHPELGNVSKAHIFNL
                     LLQQRLRQLNISIKSRPVELGYELRCVRPIGFDLMYCNLLGIGVKRLFDQGYSGCMVT
                     CDAKGDISPLFLKDVADENGKVKPRLVNIESEKVKMVFEWNTQYIEPEDYKEAQKYLP
                     NPEIYDFKKILHWD"
     gene            complement(3622..4122)
                     /locus_tag="PLF20_06460"
     CDS             complement(3622..4122)
                     /locus_tag="PLF20_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOX80680.1"
                     /translation="MDEQKNIRPSWDEYFIEVANTVANRATCDRGRSGCVIVRDKQIL
                     VTGYVGSPVGFPHCDEVGHLLKKVIHEDGTITTHCMRTVHAEQNAIAQAARRGIALEG
                     GTIYCRMTPCRTCAMLIINCGIKRVVCEKKYHTGQESEEMFAKAGIKLDYVSTELMKY
                     DNQRDE"
     gene            complement(4127..4861)
                     /gene="gldF"
                     /locus_tag="PLF20_06465"
     CDS             complement(4127..4861)
                     /gene="gldF"
                     /locus_tag="PLF20_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     permease subunit GldF"
                     /protein_id="HOX80681.1"
                     /translation="MFTLFKKEINAFFNSIIGYIVLAVFLVVNGLFLWVFPLDFNILD
                     FGYANMDGLFILTPFVFLFLIPAITMRTFADEKSKGTIETLFTKPLTDMQIILAKYFA
                     SFLLVVLSLLPTLIYFFSVYQLGMPRGNLDSGAIWGSYIGLLFLGASFTAIGVFSSSI
                     TDNQIIAFIVGAGLCAFVYLGFEFIYGLALFGKIDLFIKSLGMNAHYASMSRGVIDTR
                     DVIYFFSVIVIFLLLTQFVLERRKWK"
     gene            5093..6334
                     /locus_tag="PLF20_06470"
     CDS             5093..6334
                     /locus_tag="PLF20_06470"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HOX80682.1"
                     /translation="MKAEIITIGDEILIGQIIDTNSAWIANELNLIGIDVCRMISIAD
                     QADEIIRAVDDSFQRVDVIVMTGGLGPTSDDKTKQVLCDYFQTHLLFDEEAYKDVEKF
                     FSDRNLEVNEFNRQQAMLPENAINFPNSMGTARGMWFTKDGKHLFSLPGVPYEMKAIM
                     QQSVLPELKKLSGLQFIFHKTIMTTGIGEAFLAEKIHTWETELPSFIKLAYLPSPGLV
                     RLRLSAKGSHPEIVQNAVMQKVEELEKIIPEYIYGFDDEPLEKVVGDILKQKEKTLCT
                     AESCTGGYISHLITSIPGSSDYFKGGVVAYSNEIKAEILQVDENLLKEYGAVSQQVVE
                     AMAINARRLLQSDFSIAVSGIAGPDGGTPQKPVGYVWIAVADAQTCKSEVFHFGNQRM
                     QNIQRTALSALNLIRLMLIYP"
     gene            6440..6682
                     /gene="rpmB"
                     /locus_tag="PLF20_06475"
     CDS             6440..6682
                     /gene="rpmB"
                     /locus_tag="PLF20_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOX80683.1"
                     /translation="MARICEITGKQVIVGNKVSHSNHKTKRKFYSNLQSKKFYVPEED
                     AWITLKVSAAGIRTIHKKGIYAALKDASRKGIIVVE"
     gene            6863..9427
                     /locus_tag="PLF20_06480"
     CDS             6863..9427
                     /locus_tag="PLF20_06480"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX80684.1"
                     /translation="MKTKPLFIADFRIFILFISIVLTSYTLFSQKKPMAQVSGYISNQ
                     HGEPLELVNISIEGLPGGTSTDRNGFFQLRVPAEIEVNLIVSFIGFKTERIPLHLHQG
                     EKITIRRTLEIQNTLLSPVSIEDHQIRSSSLTRIDPKAATTIPTLGGGIEALIKTMPG
                     VTSSNELSSQYSVRGGNYDENLVYVNDVEIYRPFLIRSGQQEGLSFLNPDLVSSILFS
                     AGGFEAKYGDKMSSVLDIQYKKPAQFGGSASLSLLGGSFQIEGISKNKKLNYLLGVRQ
                     KTNQYILSSQQTKGDYKPSFTDVQANVNYNLHPRLEISALGNFSINLFRLVPHDRETS
                     FGTIQEAYRLRVFFEGQEDDKFVNGMGAITATWKPCDPLRLKFITSAFRTVETETYDI
                     LAQYWIGDLETDQTNGGSGKVVEERGVGTYLNHARNRLEATVASFEHKGTFLLPNNNY
                     MNWGVKYQHESIKDKLNEWTLIDSAGFALPLTIDSVGYTHPQIQPPLQIPMNEYLYSK
                     NTLNSNRLSAYFQRSWNFISDSTKFSLTAGIRSQYWDLNHQWLFSPRATLSITPNWKK
                     DIIFRISTGYFYQPPFYRELRDYQGIIHTDVKAQKSVQVVAGADLNFKAWSRNFKFVA
                     EAYYKYFDHLIPYDIDNVRIRYYAKNNAHGYAAGIDLKVNGEFVKGIESWASLSLMKT
                     MEDIEDDYYWEYYNQAGEKIIPGFTTDTRAVDSIKIEPGYLPRPSDQRVSFNLFFQDY
                     LPNNPTYKMHLNLVFGTGLPFGAPKSPRYQHIYRTPSYRRVDIGFSKLLKSEASNAPG
                     FLQYFKSIWITAEVFNLLQFNNTVSYSWISDVNGRRYAIPNYLTPRLVNLRLIFNF"
     gene            9438..10076
                     /locus_tag="PLF20_06485"
     CDS             9438..10076
                     /locus_tag="PLF20_06485"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOX80685.1"
                     /translation="MQRGLIVFSMKDLERMKERVAHLPMKIEENEISVLPVAEYQGEI
                     VVIERLRNLSQALEEIYSWGYVGFDTETRPNFHKGENHKPALMQVAGARKTFLFRLNK
                     LGFPPLLRSFISDENIIKVGLALENDLPELTLHQKFSPAGMVDLNQLCPQLGFQNIGA
                     RKLTAIFLKVRLKKSQQTSNWENSILTEAQLKYAALDSWICREIYSILMGIV"
BASE COUNT         3148 a         2171 c         2012 g         2920 t
ORIGIN      
        1 ccatgatcat tgtttcgcac gacagggatt ttttacaggg actcacccat agagttatcg
       61 aattcaaaca acatcatatt aatgaatatc tgggcgatat ctatgatttc ctctcggccc
      121 gccaaattga tactctccgt gagctcgata ctgaaaataa aatcatccaa aataatcaaa
      181 tcctacagca aagccagaat aagatagaat acgaaaaacg aaaagcacac gagcgtaaaa
      241 ggagaaaaat tatttcagag atcgaaaaat gtgaagaggc ggtttttcaa accgataacg
      301 agttggccga gatggaacac aaacttgcca accctgctca atatcaggat accatcaaca
      361 gtaaagattt cttcaacaga tatgattatc tgaaaaaaaa gtcagaacgc ttaatgacag
      421 aatgggaaga tttgcatctt atgctcgaag agcttgacca ctcagacctg acggacagct
      481 aaacgctcga taagtgtttt caatccaatt tttctattgt cttcaactgg caattcatcg
      541 agcaggcgaa gggcgtcgct aaaatatcca tcggccaagg cataaccatc atcacccact
      601 tttaaatcat cataaatatg ttttactctc gatatttttt catcggcggg catggtattg
      661 tttccgaaac attcatccag ttcgatgcgt tgggatggat tggctttttg ataagcttgc
      721 agaaacaaat atgtgcgctt attggtcata atatcattac cgatactttt cccgaactta
      781 tcctgttgag cataacaatc taggatatcg tccattagct gaaaggctaa acccattttt
      841 attcccatct gataaagctg ttcggcctgt tgttttgggg ttctggccac tatagctcca
      901 gtcatcagtg aagtaccaag cagtactgcg gttttttccc gtatcatctc gatatactcg
      961 tctatgctca catcggttcg gttttcgaat tcgatatcga gttgctggcc ttcgcatact
     1021 tggatagccg ttttattgaa cagccgcaaa atttcaccta aaaagccttc atcggtttgg
     1081 atcagaaatt cataagcaag agcaaacatg gtatcaccca caagaatggc aacattagta
     1141 ccccatttcc tatacacggt ttcatgattt cgtcgtatag gcgactggtc catgatatca
     1201 tcgtgtacta atgtaaaatt gtgaaagatc tcgattccta tggccggtcg tagaattttt
     1261 ttcaaatctc cgccgaacat attgcaggca attaaagcca gagcaggtct gagcctttta
     1321 ccattctgat gcatcatata agcaatggga tcataaaggt tagaagggta accgctaaag
     1381 gggagacttt cgatttcctt attgatgagt tcatgtattt gttctatact cttgatctcc
     1441 gtgtccattt tagttgattt tgagttttat tcggtttcag caattcgttg taaatatcgt
     1501 ccataaccgc ttttgagcag aggttcggct aactcaagga gttgatcttt agtgataaat
     1561 ccgcgacggt aagccacttc ttcgatactt cctatcaata gaccttggcg attttggatt
     1621 acttcgacaa actgagaagc ctgagtcagc gattcgaatg tccctgtatc gagccatgca
     1681 ataccgcggc ttagcaaacc tacctttaat ttgcctttca cgagataatg gcggtttaca
     1741 tctgtaattt catattctcc ccgtgcactt ggttttatat tcttagctat tttcactaca
     1801 gaattatcgt aaaaatacaa acctggcaca gcatagtttg agcgtggtac cttaggtttt
     1861 tcttcgatgc tgatggcctt gaaattctca tcgaactcta ctacaccata ccgctcggga
     1921 tcgcttacat gataggcaaa aactatccct ccttcaggat cgttggaagc taaaagcagt
     1981 tcttgtaacc ctcgtccata aaaaatatta tcgcctaaaa tgaggcaaac cttatcggac
     2041 tcgataaatt tttctccaat gacaaaagcc tgtgccaaac cattaggtat ttcctgaacg
     2101 gcatacgaaa accggcatcc tatctgagaa ccatcgccca acaatcgctc gaaattgggt
     2161 aaatcattag gagtggaaat gatgagaatt tccctgatgt cggccatcat caatacggat
     2221 aatggataat agatcatggg tttatcataa acaggcatca attgcttact cagggccaat
     2281 gtaagcggat gaagcctcgt acccgagccg ccagctaaaa taattccttt catctatgag
     2341 attttaaatc gtgaatttca agcatacatt taaaaacaga tgatcaatcc caatggagaa
     2401 tttttttaaa atcatatatt tcagggttgg gtaaatactt ttgagcttct ttataatctt
     2461 caggctcgat gtattgggtg ttccattcga aaaccatttt taccttttcc gattcgatat
     2521 ttaccaggcg aggttttact tttccatttt catcggctac atctttcaga aatagtggag
     2581 agatgtcacc ttttgcatcg caagtgacca tacaacccga ataaccctga tcaaacagtc
     2641 gtttaactcc aattcctaaa agattgcaat acataagatc gaagcctata ggccgaacgc
     2701 atcgcaattc gtaacccagc tctacagggc ggctcttgat ggaaatattc aactggcgaa
     2761 gcctttgctg gagcaataga ttaaagatat gtgccttgct tacattgccc agttcaggat
     2821 ggccatgttc atcgaaagta aaattgatgc ctgtgctcaa aatttcttcg tcgctcagga
     2881 aatgaaacac accttcgcta atgatagccc caccgtaaga tatgcctagt agtttacgtt
     2941 ttatcatggc tgagataatc aaacgcagaa ttttatcgat agttatctcg gttttattga
     3001 acatctcggg aatgataatc ataggataat gacatgaggc tccaatgccg aaagccaaat
     3061 gtccggcttc acggcccatg gccgacacga tgaaccagtt tccgctagtg cgggcatctt
     3121 cataaaccgt ggtagccagc ctcactcctt cttccttggc cgattcataa ccaaaagtgg
     3181 tttgcccttc cggaagtggc aaatcattgt caatggtctt aggaacatgt atgtttttta
     3241 catcgaggcg gttttcttga agaaacatcg aaattttatt ggccgtcgag gccgtgtcgt
     3301 cgcctcctat ggttaccagc agttttacat tatgttttgt gaaaaaacgg gtattgaatt
     3361 cttcttcttt aggcttgtag cggctcattt gcaaaaatga tccacccgtt ttgtaaatgt
     3421 tgtcggccag tcggaactca atatcggtga tattaggttc atccgaaaac aaggttttat
     3481 agccttcgtg tattcctatg acatgatagc ccgactttaa aaaaacttta cttactgttc
     3541 caattacagt attaaccccc ggagcagggc ctccccccgt caaaattacg atcgattctt
     3601 tcatacaaaa ttctttaata attattcgtc tctttggttg tcatatttca taagttcagt
     3661 gctcacataa tccagtttga tgcctgcctt agcaaacatt tcttcacttt cctgaccagt
     3721 gtggtatttt ttttcgcaaa ccactctttt tatgccacag ttaatgataa gcatggcaca
     3781 agtccggcaa ggggtcatcc ggcaataaat ggttccgcct tcgagggcta ttccgcgtcg
     3841 ggctgcctgg gcaattgcat tctgttcggc gtgtactgta cgcatgcaat gagttgttat
     3901 cgttccatct tcatggatga ccttcttcaa taaatgtccc acttcatcgc aatgaggaaa
     3961 acctacaggt gagcctacat aacctgtaac cagaatttgt ttgtcgcgta cgatgacaca
     4021 tcccgaacgg cctctgtcgc aagtagcccg attagctacc gtattggcaa cttctatgaa
     4081 atattcgtcc cagcttggac gtatattctt ctgttcatcc ataagattat ttccattttc
     4141 gacgttcaag aacaaattgc gtaagaagta aaaaaattac gattacactg aaaaaataga
     4201 ttacatcacg agtatcgatg acacctcggc tcatcgaagc ataatgagca ttcataccca
     4261 gagatttgat aaataaatcg attttcccaa acaaagccaa accatagata aactcgaaac
     4321 caaggtagac aaatgcacat aaaccagccc cgactataaa ggcaataatt tgattgtcgg
     4381 tgatgctcga tgaaaatacg ccaattgccg taaaagatgc acctaaaaac aataaaccaa
     4441 tataggaacc ccagatggct ccactatcca aattgcccct gggcataccc agctgatata
     4501 ccgaaaagaa atagataagt gtgggcaaaa gggaaagaac aactaataag aaacttgcaa
     4561 aatattttgc taaaataatt tgcatgtccg taagcggttt tgtaaataag gtttcgatgg
     4621 tgcccttgct tttctcatcg gcaaatgtac gcatggtaat agccggaatc aggaatagaa
     4681 agacaaaggg tgtcaaaatg aataagccat ccatattggc ataaccaaaa tcaagaatat
     4741 tgaaatcgag cggaaatacc cataaaaaca agccattcac caccagaaag acggctaaaa
     4801 caatgtagcc gatgatggaa ttgaagaaag cattgatttc ctttttaaac aatgtaaaca
     4861 taaactttca gcctcttaaa gtattacacg attcctttct cgattggcag gattcaatta
     4921 atcagtcagg tgcaaaacta taaaatttgc ataagtaaag aaaaaatgta tccatttaat
     4981 gaccaagatc cgacacaaca gaaatgaatg ggaggattga cttgtttagt gaaaaacagc
     5041 aatatgataa agcattgtag atataaattt gcacatcgaa ttataatgaa ccatgaaagc
     5101 agaaattatt accataggcg atgaaatatt gataggtcag atcatcgaca cgaattcggc
     5161 atggatagct aacgaattga atttgattgg tatagacgta tgtcgcatga taagtatagc
     5221 cgaccaggca gatgaaatca ttcgagccgt cgacgatagt ttccagcgag tcgatgtaat
     5281 tgttatgacg ggggggctgg gccccacaag cgacgataaa accaaacagg tgctttgtga
     5341 ttatttccaa acgcatttgc tgtttgacga agaagcttat aaagacgtgg agaaattttt
     5401 cagcgatcgt aatcttgagg tgaacgagtt caaccgacag caagccatgt tgcccgaaaa
     5461 tgccatcaat ttccccaatt caatgggaac tgcccgagga atgtggttca ccaaagatgg
     5521 gaaacatttg ttttcgttgc cgggtgtacc ctacgaaatg aaagccatta tgcagcaatc
     5581 ggtacttccg gaactgaaaa agttgagcgg cctgcaattt atcttccata aaacgattat
     5641 gactacagga attggcgagg ctttccttgc cgagaaaata catacatggg aaacggaact
     5701 gccttctttt atcaaactgg cttatttacc ttcgcctggg cttgtcaggt taaggttatc
     5761 ggctaagggc agccatcccg aaattgttca aaatgccgtg atgcaaaaag tagaagagct
     5821 cgaaaagatt atacccgaat atatttatgg tttcgatgat gaacctctcg aaaaagttgt
     5881 aggcgatatt ctgaaacaaa aagagaaaac cttatgcacg gccgaaagct gcacaggagg
     5941 atatatttct catctgatca ccagcattcc gggaagttcg gattatttca aaggcggtgt
     6001 agtagcctat tccaatgaaa ttaaggctga aattctccag gttgacgaaa atcttttaaa
     6061 ggaatatgga gcagtaagcc agcaggtggt ggaagccatg gctataaatg cgcgtcgatt
     6121 gttgcaaagt gattttagca tcgctgtgag tggcattgct ggccccgatg gaggaacacc
     6181 ccaaaaaccc gtgggatatg tttggatagc cgtggcagat gcacaaacct gcaaatcgga
     6241 agtattccac tttggcaacc aacggatgca gaatattcaa cgtactgccc tgtctgccct
     6301 caacttgatt cgccttatgc tcatctatcc ttgattttgc atactttaaa aaaaattctg
     6361 ctctaaatct tttttattcg aaaattttac cgaaatttgc actcccaatt aaagagtaga
     6421 atcttttaaa gatcgaatta tggctagaat atgtgaaatt acaggaaaac aggtaattgt
     6481 agggaataag gtttcccact ccaatcataa aaccaaacga aaattttatt ccaatctgca
     6541 atcgaaaaaa ttttacgtac ccgaagaaga tgcgtggata actctgaaag tttcggctgc
     6601 tgggatacgt accattcata agaagggtat ttatgctgcc ttaaaggatg ccagccgcaa
     6661 aggaatcatc gtggttgaat aaagaaaaca agaaaaatag tgaattgcaa aaaagtcgct
     6721 gtaaaattcg gcgacttttt attttgttta ccaatgtgta cttttttatc aactaagccg
     6781 tttaaagatg aaaggtttgg caactaattg ccaaactttt aattttggcc tctcgcaaac
     6841 ctattttcaa cagaaactga aattgaaaac gaagccttta tttattgctg attttcgaat
     6901 atttatcctg tttatcagta ttgttttaac ctcgtataca cttttcagtc agaaaaagcc
     6961 aatggctcag gtttcgggct atatatcgaa tcagcatgga gaacccctcg aactcgtaaa
     7021 tatctctata gaaggattac ctggcgggac ttcgactgac aggaacggct tttttcaatt
     7081 acgggttccg gccgaaatcg aggtgaatct gatagtttca ttcataggtt tcaaaaccga
     7141 acgcattcct ctccatcttc atcaaggtga aaaaatcact attcgtcgca cgttggagat
     7201 tcaaaacacc ttactttctc ccgtttctat cgaagatcat caaattcgtt catcaagcct
     7261 tacccgcatc gatccaaaag cggcaactac cattcctacc cttggcggcg gaattgaagc
     7321 tctcattaag acgatgccgg gcgtaacttc cagtaatgaa ttgagttcgc aatactcggt
     7381 aagaggaggc aattacgacg aaaaccttgt ttatgtgaat gatgttgaaa tttataggcc
     7441 tttcctcata cgttcggggc agcaggaagg attgagtttt ctcaaccccg accttgtatc
     7501 ttctatctta ttttcggcgg gtggtttcga agccaaatat ggcgataaga tgtcgtcggt
     7561 gctcgacata caatataaaa aacctgcaca gtttggaggc agcgcttcct tgagcctgct
     7621 gggcggctca tttcagatag aaggcatctc gaaaaacaag aaattgaact atttgctggg
     7681 tgttcgtcaa aaaaccaacc agtatattct ttcttctcag caaaccaaag gagattacaa
     7741 accctccttc accgatgtac aagctaatgt gaactataac ctccatccac gtctcgaaat
     7801 ttcggcactg ggtaactttt ccatcaatct tttccgctta gtacctcatg atcgtgaaac
     7861 atcatttggt accattcagg aagcctatcg tttgagggtc tttttcgaag gtcaggaaga
     7921 cgacaagttt gtgaatggca tgggagccat tacggctaca tggaaaccct gcgatccttt
     7981 acggctgaaa ttcattacct cggcattcag aaccgttgaa acagaaactt acgacatttt
     8041 agcacaatac tggataggtg atttggaaac agaccaaacc aatggaggct caggcaaagt
     8101 ggtcgaagaa cgtggagtag gtacttacct caatcacgct cgcaaccgcc tcgaagccac
     8161 cgtagcctca tttgagcata agggaacttt tctgctgccc aataataatt acatgaactg
     8221 gggtgtaaaa tatcaacacg aaagcatcaa agataaactc aacgaatgga ctttgatcga
     8281 ttcggcaggc tttgccctcc cacttaccat cgattcggta ggttataccc accctcaaat
     8341 tcaacctccc ctccaaattc ccatgaacga atatctttat tcaaaaaata cactgaattc
     8401 taaccgttta tctgcttatt ttcagcgttc atggaacttc atttccgaca gtacgaagtt
     8461 ttcacttact gcaggaataa ggtctcaata ttgggacctc aatcatcaat ggttatttag
     8521 cccaagagcc acgctttcca taacacccaa ctggaaaaaa gacatcatct ttcgaatttc
     8581 gacgggatat ttttatcaac ctccatttta tagggaactg cgcgattatc agggaatcat
     8641 tcatacagat gtaaaggctc agaaatctgt tcaggttgtg gcaggtgccg atttgaactt
     8701 taaagcatgg agtcgaaatt ttaaattcgt tgccgaagct tattacaagt atttcgatca
     8761 tcttattccc tacgacatcg acaatgtaag aatccgctac tatgcgaaga acaatgcgca
     8821 tggatacgca gcaggaatcg atttgaaggt aaatggtgaa ttcgtaaagg gaatagaatc
     8881 atgggcaagc ctttctctga tgaaaacgat ggaagatatt gaagacgact attattggga
     8941 atattataat caggcaggtg aaaaaattat tccaggattt actaccgata ccagagccgt
     9001 tgatagtata aaaattgagc caggttattt acctcgtcct tccgatcaaa gagtaagctt
     9061 taatctgttt tttcaggatt accttcccaa taatcccacc tacaagatgc atttgaatct
     9121 ggtttttggc acaggacttc cctttggagc tccaaaatcg ccccgttatc agcatattta
     9181 ccgcactcct tcctatagaa gagtcgatat tggtttttcg aaactcctca aaagcgaagc
     9241 ttccaatgca ccaggatttc ttcagtattt caaatctatt tggattaccg ccgaagtgtt
     9301 taatcttttg caattcaata acacagtttc ctattcatgg atttccgatg tcaatggcag
     9361 aagatatgcc atacctaatt accttacacc acgtttggtc aatctgcgct tgatattcaa
     9421 tttttaatcc aagggaaatg caaagaggcc tgatagtatt ttcaatgaag gatctggaaa
     9481 ggatgaaaga aagagtggct catcttccga tgaaaataga agaaaatgaa atatcggtct
     9541 tacctgtggc tgaatatcaa ggagaaatcg tagtcataga acgattaaga aatctaagtc
     9601 aggctttgga agaaatctat tcatggggtt atgtaggttt cgataccgaa acacgtccga
     9661 attttcataa gggagaaaac cataaaccgg cccttatgca agttgccggc gctcgaaaaa
     9721 cctttctgtt tcggctcaat aaactggggt ttccaccttt attgcgctcg tttataagcg
     9781 acgaaaacat cataaaagta ggcttagccc tcgaaaatga tcttcccgaa cttacactcc
     9841 accagaaatt ttcgcctgcc ggcatggtcg acctcaatca attatgtcca caacttggtt
     9901 ttcaaaatat tggagcgaga aaattaaccg ctattttcct aaaagtaagg ctcaaaaaaa
     9961 gtcagcaaac aagcaactgg gaaaattcaa tccttaccga agcacaattg aaatacgcag
    10021 cactcgattc ttggatctgt cgtgagatat attctatcct catgggtata gtataaaccc
    10081 agagattaac gattccttca ttttttcaac tgtccaaatc tatacgccat catttttgat
    10141 gcatagatgt tgaagcttat cgatgaaacc aaaagcaacc gctgaatctc ttctaaagac
    10201 aagattcaat gagtagggtc tgacattcat cttacattga ttgggtaggt a
//
LOCUS       DAOORU010000085        10233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8166_length_10233_cov_5.796718, whole genome shotgun sequence.
ACCESSION   DAOORU010000085 DAOORU010000000
VERSION     DAOORU010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10233
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8166_length_10233_cov_5.796718"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PLF20_06490"
     CDS             <1..961
                     /locus_tag="PLF20_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HOX80686.1"
                     /translation="VSKISYTDISGDSWEEYNVFHHGFIRYLQVKGYNPDEVLQLSDK
                     EIDKLIEDSPYYNSTANDIDWVAKVRMQGNIQKWVDHSISVTVNVPAETSEELVSKIY
                     LTAWESGCKGVTIYREGSREGVLIKEKKEEEMVIHENNAPRRPKVLEASVVRFLNGSE
                     EWVAVVGIYNGRPYEIFTGKAEGFYAPQWVTHGWVIKNRQPDGKARYDFQFEDKDGYR
                     TTMEGLSRMFDAEYWNYAKLISGILRHGMPLTSVIDLVSKLRLDSDSINSWKTGVERA
                     LKQFIPNGTKVAKATCEKCGQTGTLQYQEGCLICTSCGYSKCG"
     gene            1343..3244
                     /locus_tag="PLF20_06495"
     CDS             1343..3244
                     /locus_tag="PLF20_06495"
                     /inference="COORDINATES: protein motif:HMM:NF013781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOX80687.1"
                     /translation="MAEKDSNTGKFFEEFKPVSTEEWEEAIRKDLKGADYEKKLVWQS
                     PEGFKVMPYYREEHLEGIPFIHSLPGIFPFVRGNKKTDNTWKIRQDIAIDKDNFENSL
                     QKAKTIIQKGVNSIGFRVAEEFSIEYSDFEKLMTQIWQNGIEINIEAGLQTPQIAEFL
                     SKYAQNNKLPFEKLEGCLSFDPLIHVLLNGNYPVGEDEISLFDKARQIIETTKDFPKV
                     KSLTISGYLFGNSGSSIVQELAFSLSAAVEYFHRLTDLELSVSDISNKMRFIFSIGSN
                     YFFEIAKLRAARMLWARVVNEFRPSDMDSCKMNIHCVTSAYNKTLYDPYVNMLRTTTE
                     AMAATLGGTDSLEVLPFDFPSGKTTEFSERIARNQQLILKEEAGFDKIADPSAGAYYI
                     ENLTDNIAREAWKLFLEIENQGGYYQALRVGKIQEMISGNALKREAAIASRKETLVGT
                     NQYPDLNEYIDKELDPSIYTKPQIPTAAYTIQPLIPYRGSMPMEELRYKTDFYSKNHK
                     RPAVFLLLIGNVAMRQARAQFSSNFFGCAGFQIITGNPVASVDEGIRAAHESKAEIVV
                     ICSSDEEYIQYAPAIYQELKDETLVVVAGNPPSAEELKSMGLTHFIHLRSQLLDTLRQ
                     YQKDLHIVS"
     gene            3401..5545
                     /gene="scpA"
                     /locus_tag="PLF20_06500"
     CDS             3401..5545
                     /gene="scpA"
                     /locus_tag="PLF20_06500"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOX80688.1"
                     /translation="MKPNFEDISAFSTEQFPEVVAPAEIPSWETPENIRVKSVYTAED
                     LANMEHLQYAAGIPPFLRGPYSTMYVMRPWTVRQYAGYSTAEESNAFYRRNLAAGQKG
                     LSIAFDLATHRGYDSDHPRVIGDVGKAGVAVDSILDMKTLFDQIPLDQMSVSMTMNGA
                     VLPIMAFYIVAGLEQGVKLEQLSGTIQNDILKEFMVRNTYIYPPQPSMRIIGDIFRYT
                     SKYMPKFNCISISGYHMQEAGATADLELAYTLADGLEYLRTGINAGLQIDEFAPRLSF
                     FWGIGMNHFMEIAKMRAARMLWAKIVKQFDPKNPKSMALRTHSQTSGWSLTAQDPYNN
                     VARTCIEAMAAALGHTQSLHTNALDEAIALPTDFSARIARNTQIYLQEETQICRTVDP
                     WAGSYYVESLTHHIAHRAWKLIQEIEDLGGMAKAIETGLPKLRIEEAAARKQARIDAN
                     KDIIVGVNKYRLEKEEPIEVLEVDNTAVREAQIRRLNELKASRDSEAVKKALDAITKA
                     CTTGEGNLLELAVEAAKARATLGEISYACEKVFGRYEAVIKSVSGVYSLESKDDDNFR
                     KACEMADEFARLEGRRPRIMIAKMGQDGHDRGAKVVATGYADIGFDVDIGPLFQTPEE
                     AARQAVENDVHILGVSSLAAGHKTLVPQVIEELKKLGRDDILVIVGGVIPPQDYEFLY
                     EAGVAAIFGPGTVVSEAAIKILEILIKSRKAE"
     gene            5695..7296
                     /locus_tag="PLF20_06505"
     CDS             5695..7296
                     /locus_tag="PLF20_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HOX80689.1"
                     /translation="MKKSPQIPHTYVIVFAIIFLAAMLTWIIPGGQFSRQTIRTSDGT
                     REVIDAHSFQSIDHQPQTWQIFSAFYKGFIRTPKIIVFILILGGAFWLLNESKAVDVG
                     VYSFLAKTQKFNRYKFFRKIGVNNLIIAVVMLIFSFFGAIFGMSEETIAFVIIFVPMA
                     ISMGYDSIVGVSMCFIGAGIGFAGCLMNPFTLGIAQEIAGLPLFSGIEYRFFIWIIVN
                     IFGIGFVLWYANHVKRNPRLSPVYENDQYWREQHANRQNLSLTPANKSTYLAYSLILS
                     AALVAAILLPINQIRLGNTSFKLPALPIAALLFAFFGWVSLKKSREFFMLNILGFTII
                     YLIIGVTGFSWGLLELATLFFTMAILIGIGLGKTPNQITQSFMAGAKDILSAALVVAL
                     AGGIIVILEDGKIIDTILYSISNSIQGAGKISSLSLMYLFQSAMNAIITSGTAKAALL
                     MPILRDVSDMIGLSQQATVTAFHIGDGFTNLITPTSGVLIGVLEVARIPFVKWFKWAW
                     KFILMMVILGWLLLIPTVTIPLNGF"
     gene            complement(8074..>10233)
                     /locus_tag="PLF20_06510"
     CDS             complement(8074..>10233)
                     /locus_tag="PLF20_06510"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOX80690.1"
                     /translation="RMVIRDRNHPSVILWSIGNEEGYIQTNGNGKRMAITLIEKLKRL
                     DPSRKVTYAADVPNVFNGINEVIPIRGFNYREKAMEAYHKDHPNQPIIGTEMGSTVST
                     RGIYSYDSINCYLPDLDLTAPWWASKADEWWPIVAENPWAAGGFIWTGMDYMGEPTPF
                     HWPNIGSHFGVMDQCGFPKNLYYYYQSWWDTTLPVLNLAPHWNWLNRWGFEQEVWIDS
                     NSDSVNLKLNGKNLGTRPMQKNQHLKWKVKYEPGILEAKGYKNGFEFITKMETTGPAF
                     ELVVSPNKTTAMANSTDVVIINISVVDTGGREVLTADNRVKFKVTGPAKIIGVGNGDP
                     SSHEPDKCETNLWQRSLFRGKCQVILQMGDTPGKVGFEASSEGLWPASTEIHTLPDSF
                     HHIAPTEDQIRPAGINDKERIMGADISFLPQLEAEGMKFYDFGNNIPQDVFEILKKYG
                     FNYIRLRIFYQPENPEGYSPRKGYCNLAQTLEMARRIKKAGMKFLLDFHYSDYWADPG
                     KQYKPKAWDSLPFDILKDSVRNYTIRVLRALQAQNTLPDMIQPGNEINHGLLWPEGHI
                     SQPDHLADLLQASISGIKEVAPDIPIMLHLALGGQNDESVFWLNNMFARGIDCDVIGL
                     SYYPRWHCTLDDLKYNMRDLIRRYGKDIIVVEYSQKKEEVAGAVFGMPNDKGKGCFIW
                     EPLNTWEGIFDKNGKPTRWLHAYENIKAMYLDRNLNK"
BASE COUNT         3079 a         2089 c         2156 g         2909 t
ORIGIN      
        1 tgtcagtaaa atctcctata ccgatatatc gggcgattcc tgggaagaat acaatgtatt
       61 tcatcatggt ttcatccgat atttacaggt taagggttac aatcccgacg aggttttgca
      121 actctccgac aaagaaatcg acaaactcat cgaagattca ccctattata attcaacagc
      181 caatgatatt gactgggtag ccaaagttag aatgcaggga aacattcaaa aatgggtaga
      241 tcattccatt agcgtaacgg taaatgttcc tgccgagaca agcgaagaac tagtgagtaa
      301 aatatattta acagcatggg aatcgggttg caaaggtgtt accatttatc gtgaaggatc
      361 cagagaaggt gttctgatca aagaaaagaa agaagaggaa atggtgatac atgaaaacaa
      421 tgccccgcgt cgtcctaaag tactggaagc cagcgtagta agattcttga acggctctga
      481 agaatgggtg gcagtggtgg gaatttataa tgggcgacct tatgaaatct ttacgggaaa
      541 agcagagggc ttttatgctc cacaatgggt tacccatgga tgggtcatca aaaatcgcca
      601 acccgatggc aaggctcgtt acgactttca gttcgaagat aaggacggat atcgcaccac
      661 catggaaggg ctttcaagaa tgttcgatgc agaatattgg aattatgcca aactgatttc
      721 gggtatcctg cgccatggaa tgcctcttac ttcagtaata gatttggtat cgaaactaag
      781 actcgattcc gattcgatca attcatggaa aaccggagtc gaaagagccc ttaaacagtt
      841 catacccaat ggaaccaagg tggccaaggc aacctgtgag aaatgtggac aaaccggaac
      901 gcttcagtat caggaaggat gtttgatttg tacctcatgt ggttattcga aatgtggtta
      961 aaaggtgtta ttttattatg atacatcgaa ggaatcaaca aaagtgaatg tcaaggttga
     1021 ggctgcctgc catcggcagg cagcctcaac cttgacaaat caagtaggat gcctatttga
     1081 tttgatgtaa tgctctgtaa atcaatcttt tggcttctat atttattctc tattcatact
     1141 attgttgtag gtttggatgt aaaaaatcta cattttatcc cagcgaaaag ccataacttt
     1201 gcagctcatt ccagtcgttt caataaaata tgtctgtgaa atacctaaat aactaataat
     1261 gaatatttta aaacaattac atttaggttt ttcgaaaaat agaatcatca tcgtaatcaa
     1321 aacaaattaa ccatataacg aaatggcaga aaaagacagc aacactggca agtttttcga
     1381 agaattcaag cccgtaagca ctgaagaatg ggaagaggct ataaggaaag atctaaaagg
     1441 agccgactac gaaaagaaac ttgtctggca aagtcccgaa ggatttaaag taatgccata
     1501 ttacagagaa gaacacctcg agggtattcc ttttatccat tccttgcctg gtattttccc
     1561 tttcgtacgg ggtaataaaa aaaccgacaa tacttggaaa atacggcaag acattgcgat
     1621 cgataaggac aacttcgaaa actcacttca aaaagcaaag acaatcattc aaaaaggagt
     1681 caattctatc ggcttccgcg ttgccgaaga attttcgatc gaatactccg acttcgaaaa
     1741 gctaatgacg caaatctggc aaaacggaat tgaaataaat atagaagctg gacttcagac
     1801 acctcaaatt gcggaattct tatcgaaata tgcccaaaat aataagctgc cttttgaaaa
     1861 attagaaggt tgcctcagtt ttgaccctct aattcatgtt ttattaaatg ggaattatcc
     1921 tgttggtgaa gatgaaatta gtcttttcga taaagcccga caaattatcg aaaccactaa
     1981 agattttcca aaagtaaaat ctcttactat atctggatac ttattcggaa attcaggaag
     2041 cagtattgta caggaactcg cctttagttt gtcggcagca gtagaatatt tccatcgttt
     2101 aacagatttg gaattgagtg tttctgatat ttcgaacaaa atgcgcttta ttttctccat
     2161 tggttcgaat tatttctttg agatagccaa gttgcgagct gcacgcatgt tatgggcaag
     2221 agtcgtaaat gaatttcgac ccagcgacat ggattcctgc aagatgaata tacactgtgt
     2281 tacatcagcc tataacaaaa ccctgtatga tccttatgta aacatgttgc gtacaacaac
     2341 cgaagccatg gcagcaacgc tgggcggaac cgattcgctc gaggttttgc ctttcgattt
     2401 tccttcgggt aaaaccactg aatttagcga acgtattgcc cgcaatcaac aattgattct
     2461 gaaagaagaa gccggcttcg acaaaattgc cgacccctcc gcaggcgctt attatatcga
     2521 aaaccttacc gataacatag ctcgtgaggc ttggaaactt ttcctcgaaa tcgaaaatca
     2581 aggaggttat tatcaagctc ttagggtagg aaagattcag gaaatgattt cgggaaatgc
     2641 cctcaaacgc gaagccgcta ttgcctcaag aaaagaaacc ctggttggca ccaatcagta
     2701 tcccgatctg aacgaatata tcgacaagga gcttgatcca tccatctata caaaacctca
     2761 gattccaact gctgcttata ccatacaacc tcttatccct taccgtggca gcatgcccat
     2821 ggaagaatta agatacaaaa ccgatttcta ttccaagaat cataagcgtc cggcagtatt
     2881 tctcctgctc attgggaatg tggccatgcg acaggcaaga gcccaatttt cgagtaattt
     2941 tttcggatgt gccggctttc aaatcataac gggcaatccc gtagcgagtg ttgacgaagg
     3001 cattcgagct gcccatgaaa gtaaagccga aattgtggtc atctgctcat ccgacgaaga
     3061 atatatccag tatgctcctg ctatctatca ggaattaaaa gacgaaactc tggtggtcgt
     3121 ggcgggaaat cctccctctg cagaggaact gaaatctatg gggctgacac attttatcca
     3181 tctccgttcc caacttctcg atacactccg ccagtatcaa aaagatttgc atattgtctc
     3241 ttaatgataa attcctaatg acaaattatc attcattaaa aacaatagaa gtacataaaa
     3301 acttttgtat aagaattaca ataaaaaagc attgaattac tggacaagga aagtaaattt
     3361 ctgcaaaact gaataaatta aaagattgaa ataaaatata atgaaaccaa atttcgaaga
     3421 catttcggca ttctctacag agcagttccc tgaggtagtt gcaccggcgg agattccttc
     3481 atgggaaacg cccgagaaca ttcgggtaaa atcggtttat acagccgaag acctagccaa
     3541 tatggaacac ttgcaatacg cagctggtat tcctcccttt cttcgcggcc cttacagtac
     3601 gatgtatgtc atgcgtccat ggacagtccg gcagtatgca ggatattcta cggccgaaga
     3661 atccaatgcc ttttaccgtc gcaatctggc cgcagggcag aagggacttt ccattgcctt
     3721 cgatttggct actcatagag gctacgactc cgatcatcca agagttattg gagatgtcgg
     3781 gaaagcggga gttgctgtag attccatact cgatatgaaa accctgttcg atcagattcc
     3841 actcgaccag atgtcagttt cgatgactat gaacggagct gtacttccta ttatggcctt
     3901 ttatatcgta gccggactgg agcaaggcgt aaaactggag caactttcgg gcaccataca
     3961 aaacgacatt cttaaggaat tcatggtgcg taatacctat atttatcctc ctcagccttc
     4021 catgcgtatc attggagata ttttccgcta tacatcgaaa tatatgccca aattcaactg
     4081 catcagcatt tcgggatatc acatgcagga agccggtgct actgccgacc tcgagctggc
     4141 ttatacgcta gccgatggct tggaatatct gcgaactgga atcaatgcag gccttcaaat
     4201 cgatgaattt gcgccccgcc tttctttttt ttgggggata ggcatgaatc attttatgga
     4261 gattgccaag atgcgtgctg ctcgtatgtt atgggccaaa atcgtgaaac agttcgatcc
     4321 aaaaaatccc aaatcgatgg ctttacgtac acattcgcaa acatcgggct ggagtttaac
     4381 ggctcaagat ccttacaaca atgtagcacg tacctgtatt gaagccatgg cagcagcatt
     4441 gggtcacaca caaagcctgc acacaaatgc tctggatgaa gccattgcac tgcccaccga
     4501 ctttagtgca cgtattgcac gcaataccca aatctatcta caagaagaaa ctcagatttg
     4561 tcgtaccgtc gatccatggg caggctccta ttatgtggaa tcattgacac atcatattgc
     4621 gcatagagca tggaaactca ttcaggagat agaagacctg ggaggaatgg ccaaagccat
     4681 agaaaccggc cttcctaagt tgaggataga agaagctgct gcccgcaaac aggcaaggat
     4741 cgacgccaat aaggatatca tcgtaggagt gaataaatac agactcgaaa aagaagaacc
     4801 tatcgaagtt cttgaagtag ataatactgc cgtgagggaa gcccaaatta gacgcttgaa
     4861 cgagctgaag gccagtcgcg acagcgaagc cgtcaagaag gcattggatg caattaccaa
     4921 agcttgtacc acaggtgaag gcaatcttct ggaactagcc gttgaagctg ctaaagcaag
     4981 agcaaccctg ggtgaaattt cttatgcatg tgaaaaagta tttgggagat atgaagccgt
     5041 gattaaaagt gtatcaggag tttattcgtt agaatcgaaa gatgatgaca attttcgcaa
     5101 ggcttgcgaa atggccgatg aatttgcacg cctcgaaggt cgaaggcctc gcatcatgat
     5161 tgctaaaatg ggacaggatg gccatgatcg cggagccaaa gtagtagcca caggctatgc
     5221 cgatattggc ttcgacgtgg atatagggcc tttgtttcaa acccccgaag aagccgcccg
     5281 acaggccgtc gagaatgatg tacatatact cggagtttct tctctggccg caggtcataa
     5341 aaccctcgtc cctcaggtca tcgaagaact caaaaaactc ggacgcgacg acattttggt
     5401 gattgtaggc ggagttattc cacctcagga ttatgaattt ttatacgagg ccggtgtagc
     5461 agccatattt ggcccaggta cagttgtgag cgaagctgct atcaaaattc tcgaaatcct
     5521 tatcaaaagc cgaaaggctg agtaaacttt attataagat aaagacaata gcattcgttc
     5581 cgttgcattc cgatgatata aatgcgggca aagactggct tctgaaatta tcatctatac
     5641 gaattcgata aattcttcta agtttgaggg tagattttag ctgcctcatc ttctatgaaa
     5701 aaaagtccac aaatccctca tacttatgta attgtttttg ccatcatctt tttagcggct
     5761 atgcttacat ggataatccc tggggggcaa ttttcacgcc agaccattcg aacaagcgat
     5821 ggcactcgtg aagtaataga tgcacattcc ttccagtcga tcgaccatca acctcagaca
     5881 tggcaaattt tttcggcatt ttataaggga tttatcagaa cacccaaaat catcgtgttt
     5941 attttgattc ttggaggagc tttctggctg ctcaacgaaa gcaaagctgt agatgtagga
     6001 gtttattcat ttttagcaaa gacccagaaa ttcaatcgat ataaattctt tcggaaaata
     6061 ggggtcaata acctgatcat cgcggtcgtc atgctgattt tcagtttttt tggagcgatt
     6121 ttcggcatga gtgaagaaac cattgccttt gtgattattt ttgtgcccat ggccatctct
     6181 atgggctacg attctatagt aggagtgagt atgtgtttta taggtgcagg aattggcttt
     6241 gctggttgcc tgatgaatcc ctttacgctt ggaattgcac aggaaattgc gggcttgcct
     6301 ttattttcgg gaatagaata ccgtttcttt atctggatta tagtaaatat ttttgggata
     6361 ggttttgttc tgtggtatgc taaccacgtt aaacgaaatc cacgcttaag cccggtttat
     6421 gaaaacgacc agtactggag ggaacaacat gccaatcggc aaaatttaag tcttacgcct
     6481 gccaataaat ccacttacct tgcttattct cttattctga gtgctgctct tgtggcggct
     6541 attcttcttc ccataaatca aatcagattg ggaaatacaa gtttcaaatt acccgctttg
     6601 ccaattgcgg ctctgttatt tgcatttttt ggttgggtga gtttgaaaaa atccagagaa
     6661 tttttcatgt tgaatattct tggttttacc ataatctatt taattattgg tgtaacaggg
     6721 ttttcgtggg gtttgctcga gttggctacc ttgtttttta caatggctat tctcataggt
     6781 ataggtctgg gcaaaacgcc taatcaaatt acccaatcct tcatggcggg agccaaggac
     6841 attctgtcgg ctgctttggt agttgctttg gctgggggta tcattgtgat tctggaagat
     6901 ggaaagatta ttgacaccat tttatatagt atatccaatt ctatccaggg agccggaaaa
     6961 attagcagcc tctccttgat gtatctattt cagtcggcca tgaatgcaat cataacgagt
     7021 ggaacggcaa aagctgcctt gttgatgcca atattgcgtg atgtatccga catgataggc
     7081 ttgtcgcagc aggcaaccgt aactgctttc cacatcggtg acggatttac caatctcatc
     7141 actccaactt cgggagtatt aattggagtg ctcgaagtag cccgtatccc ttttgtcaaa
     7201 tggttcaaat gggcttggaa atttattcta atgatggtta ttttaggctg gttgttgctt
     7261 atccctacgg taaccatacc attgaatgga ttttaatgca gcagaattta tattatatga
     7321 acttctatta gagaatatta atgatcggct aaaactactg tattagcccc tacaaattac
     7381 gagaataata taaatataat ctatactttt atgcttatga aaaaaagaaa attcaccaga
     7441 gaggaaaatc ttcaaattat taagtaaacc tcccagatta gtgttaaaca aatccttaaa
     7501 aaataaggag tatatcaagt atcctactat tcaaagaaga agtttgaaga gatgagagaa
     7561 gaaggctttc acatgaaatg actccaatgc atttaaaacg tattcgtgaa ttggagaaag
     7621 aagtaactgt tttaaagcaa cttttaacgg tgaaggaact ggaaagcaag ttaagaaagg
     7681 cgttttaaaa aaagttagtc ttggagagaa gaagggaatg gcagcttgat acaatcatct
     7741 tggtttaaag cttgaaacca cattggtttt ggctgaaatt aagcgacatc aaaattacta
     7801 tcattccttg gggaaggaaa agaggggaaa gaccgagtga aaccacactt attgtaataa
     7861 aaaaacagag tagaacgtat acgctttaag aatgagaaca taggaaaaaa tgtcaagcgc
     7921 cgaaacattc cctcaaccat tatcggtata acaataatcg ttggttgttg cttgtcaacg
     7981 caaatttctc tagatttttt aaattttgta aaattatcta tgaaattttt cgaataacaa
     8041 gggtctaagc ctccacattg atttttataa atctcattta tttaaatttc ggtcgagata
     8101 catggccttg atattttcgt aggcatgcaa ccatcgagta ggtttgccgt ttttatcgaa
     8161 tatgccttcc caagtattca agggttccca gataaaacaa ccctttcctt tatcgttagg
     8221 cattccaaat actgccccgg ccacttcttc ctttttttga gaatattcca ccacaatgat
     8281 gtctttgcca tatcgcctta tcaagtctct catgttatat ttcaaatcat cgagggtgca
     8341 atgccagcgt ggataatacg aaagtccaat tacgtcgcaa tcaatgcctc tggcaaacat
     8401 attgttgagc cagaagaccg attcgtcgtt ttggcctccc aacgcaagat gtagcataat
     8461 tgggatgtca ggtgcaactt ccttgattcc cgaaatagaa gcctggagta agtcagccaa
     8521 atgatccggt tgagaaatat gaccttcggg ccatagcaaa ccgtgattaa tttcattacc
     8581 cggctgaatc atatcgggta acgtattttg agcctgcagg gcacgaagca ccctaatggt
     8641 ataatttctt acggaatctt tcaaaatatc gaatggtaat gagtcccagg ctttgggttt
     8701 atattgtttg cctgggtcag cccaataatc gctataatga aaatcgagaa ggaatttcat
     8761 tcccgctttt ttaattcgtc gggccatttc gagggtttgt gccaagttgc aatatccttt
     8821 cctgggggaa taaccttcgg ggttttcggg ttgataaaag attcttaatc gaatataatt
     8881 aaatccatat ttttttagga tttcgaatac atcttgaggt atgttgttgc caaaatcata
     8941 aaatttcatt ccttcggctt ccagttgcgg taagaaactg atatctgcac ccataattct
     9001 ttccttgtca tttatgcctg caggtctgat ctgatcttcg gtaggagcaa tatgatgaaa
     9061 ggaatcagga agagtatgga tttcggttga agctggccat aagccttcgc ttgaagcctc
     9121 gaagccgacc tttccgggcg tatcgcccat ttgaagaatt acctgacatt ttcccctgaa
     9181 tagagacctt tgccaaagat tggtttcgca cttatcgggt tcatgcgaag aaggatcgcc
     9241 attgcccact ccaataattt ttgcagggcc tgtaacctta aatttgaccc tgttgtcggc
     9301 tgtaagcact tcgcgtcctc ctgtatccac cacactaata ttgatgatta ctacatcggt
     9361 tgaattggcc atggcagtag ttttattcgg agaaactacc aattcgaagg caggacccgt
     9421 agtttccatt ttcgtgataa actcaaaccc atttttatat ccttttgcct cgagaatacc
     9481 tggttcatat tttaccttcc atttcagatg ctgatttttt tgcataggac gtgttccgag
     9541 gtttttacca tttaatttca aattaacact gtcggagttg gaatctatcc atacctcttg
     9601 ttcgaaaccc cagcgattaa gccagttcca atgtggtgcc aaatttaaaa cagggagtgt
     9661 ggtgtcccac catgactggt aataatagta gagatttttt gggaaaccgc attggtccat
     9721 tacaccaaaa tgcgatccta tgttaggcca atgaaaaggt gtaggttcgc ccatgtaatc
     9781 catgcctgtc cagataaatc caccggcagc ccaaggattt tcggctacta taggccacca
     9841 ttcgtccgct ttcgaagccc accaaggagc tgttaggtct aaatcgggca aatagcaatt
     9901 gatggaatcg taggaataga tgccacgtgt actaacggta ctccccatct cggtgccaat
     9961 aataggctga tttgggtgat ccttatgata ggcttccatg gctttttcac gatagttgaa
    10021 accacgaatg ggaattactt cgttgatccc attgaaaaca tttgggacat cggcagcata
    10081 agtaacctta cgtgatggat ccaaccgttt taatttttca attaaggtaa tggccatgcg
    10141 tttaccgttt ccatttgttt gaatataccc ttcctcattc ccgatcgacc acaaaatgac
    10201 cgaaggatga ttcctgtcgc gaatcaccat tct
//
LOCUS       DAOORU010000086        10087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8299_length_10087_cov_3.828449, whole genome shotgun sequence.
ACCESSION   DAOORU010000086 DAOORU010000000
VERSION     DAOORU010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8299_length_10087_cov_3.828449"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLF20_06515"
     CDS             complement(<1..663)
                     /locus_tag="PLF20_06515"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOX80691.1"
                     /translation="MNKFSKFFLIFAGMSFVLASCTKDLDTVPIDKDVVTSAQVYENP
                     KAYKQILAKIYAGLAVSGQQGPSGMPDISGIDEGFGQYLRGYWYHQELTTDEAIIGWN
                     DQTIKDFHWQTWGAGDVFISAFYYRIFYQISLCNEFIRETTDAKLDERNVTGSLRTDI
                     EHYRAEARFMRALSYYHALDMFGTVPFVTEKDPVGSFFPPQISRKDLFDYIEKELLEI
                     EPL"
     gene            complement(684..3677)
                     /locus_tag="PLF20_06520"
     CDS             complement(684..3677)
                     /locus_tag="PLF20_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX80692.1"
                     /translation="MMKHANLLKVLFLFFFAAVALPSFAQQLTVKGKVTDASDGTTLP
                     GVTIVVKNTTTGTVTDINGNYSITVPKGSTLAFSFVGYQTQEFVIETSQTLNVALSPT
                     ITSLSQILVIGYGQVKKGDATGSITAVTTQDFNRGSITNPIELMAGKTAGVQIVTNSG
                     APGAGATIRIRGGSSLSASNDPLFVIDGVPIDNTSIYGTRDPLSTINPNDIETFTILK
                     DASAAAIYGNRASNGVIIITTKKGTLAPGKKAPQFEYNGGFSLYTIPKKREVFDGDQF
                     RSIINERYANRPEVLALLGDANTDWQEEIYRSSFAHDHTVSATGAIGALPYRASVGYA
                     NYDGILKTDNMQRTTFGLNLNPSLFDDNLKINLNGKGMFIDQRFANEDAIGSAIQMDP
                     TKPVHDSNAYGGYWTWLQANGNPVDQATDNPVALLEQKHDNADVSRFLGNAQFDYKMP
                     FLPELRANLNLGLDYSKTNGTVYVPGNAAFAFDPQYGGGTNTQYTQEKRNELLDFTLN
                     YVKEVPTIFSKFDVMGGYSWQHFYREDTNSTTNDLNGPFFIQKRIFDTSSNKSEFYLV
                     SFFTRFNYSLFDRYLLTFTLRDDGSSRFSKDNRWGLFPSVALAWRIMDEPWMKNMKTL
                     SQLKLRLGWGITGQQNITNDDYPYLPRYTSSWATASYILGTDTLITLRPEGYDPNIKW
                     EETTTTNIGLDYGFLNDRIYGSLDVYWRKTKDLINFIPVPAGSNLSNYITTNIGDLEN
                     KGVEFAINGKPFVGPDFYWDLGVNATYNKNKITKLTASDDPNYLGVLVGTIAGGVGNT
                     IQIHSVGYPSNSFFVYEQVYDANGKPIEGLYVDRNKDGKINDDDRYHYKKPAADVYLG
                     INSRMQYKNWDFIFSGRANIGNYVYDNFSSNNSTYERLYRPEGPYLANVSADVAETGF
                     KVPQYLSDYYIKNASFFKLDNLAIGYTFDKLFNSSAKLRLSATVNNVFTITNYKGLDP
                     EIFSGIDYSMYPRTRVFVLGLNLIF"
     gene            4006..5013
                     /locus_tag="PLF20_06525"
     CDS             4006..5013
                     /locus_tag="PLF20_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOX80693.1"
                     /translation="MQNKVTIKDIAKTLGVSISTVSRALKDHPDISESTRNLIKNFAQ
                     EINYRPNVLALSLRRQKSNTIGLIIPEIVHHFFSSVISGMEDLSYREGYNIIICQSTE
                     NQFREATALQTLIDHRVDGILVSLSKSTQNFSQFKKAQEEGFPMVFFDRICESIETDR
                     VVMDDLEGGRLATEYLINKGCQNILHLAASPILLIGRNRRDGYLQALKDHNIPIKEEL
                     ILKCDTQEEVSTLRNHLYELLPQIDGVFAVNDSTAIAMIQILKKKGYRIPQDIKVVGF
                     GDGPNATIVEPALTTIEQKGYDMGRESVRLLLQRLNNPSASISYQTRVFPPTLKIRES
                     A"
     gene            5486..6745
                     /locus_tag="PLF20_06530"
     CDS             5486..6745
                     /locus_tag="PLF20_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80694.1"
                     /translation="MKHLFPLLSLVLLVSLFNIGCSKDEDEELPKDLVVNYTASFTDV
                     ASTLQDFIMKAQVYESSNFESLTADQGKKIINDYITAGENFIKSVDDYEKYKIGTKNS
                     SPWLKNSMDSPCSMYDAIPDVGNGISPGFVKSVGDLIADTKGDVKKIEKKFNDGEISE
                     EVFTAALDSLKKIKTLKAANFGMSSIIGAGTSGLTGAAIFSVGGIGMAMSAPALLGVT
                     AAGVVVGTGYYLISNWWSGIKKNGQDDKLGEIVVASGKIGDPIPTTMLSNGASLTVAI
                     SGYAPVYIPAFILPSQGIKKTLEITPIKLEDATPTTKVEVCTLEEEFTSENCEDVLYV
                     TATPSPIDPGPGEGVTVTATIFPPIAGCQISFSIVGTDGYTKSETHETDANGKASFFI
                     PGGAEGVFDIVTITTSNGHTYTVTYTF"
     gene            6845..>10087
                     /locus_tag="PLF20_06535"
     CDS             6845..>10087
                     /locus_tag="PLF20_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOX80695.1"
                     /translation="MKTRHLLRKTFIIGLVVFSLPRLYSQNGFTENKGQWDSNVLFFL
                     QPQADVAVFIEAGAITFNIQLLRHDETPNFENISAKTNHEGVKGHAFRIIFEDADFSN
                     ITALRPKPELLNYFLGKNPEKWSTGVKIFNELYIYDAYKNIDLKIISDIGGIKYEFII
                     HRGGRVKDIAIRYDGLENISLSNGQLILSTSLGEIIEEAPKVIVEDKGSTFVTGCQYQ
                     LEANRIKFHLDPSFLNSDKIVIDPRLVFSTYSGSEADNWGFTGTYDEAGNVYSGGIVF
                     NIGYPVSLGAYQQSFGGGCDIGIIKYDSTGTNRIYATYLGGLYADLPHSLIVNTSNEL
                     VIFGTTGSPNFPVTPSAYDTSFNGGDAVNYASLNFPLGSDIFIAKFSEDGSQLIASTF
                     IGGSENDGLNFRSRYASMLMTGNDTLYYNYGDGARGEVITDPQDNIYIGSTTFSNDFP
                     ITAATFQPSYKGKQEGVVCKFDRTLSYLNWSSYLGGSNDEGIFSIDINSYGDLYVTGG
                     TTSSDFPVTSGAYRTQFNGGSADAFISCISANGVNLKGSTFWGSLSYDQGYFVRIGPD
                     DEVYVFGQTKASDSSMMIDALYGQPNSGQFISKFSPLLDELRWSTVVGKGDGQPNISP
                     TAFMVDVCGRIYYSGWGRIWGNSYVNGNWYLWGTPFGTHGLAVTPDAVQPITDGQDFY
                     IAILSSEAAGLDYATFFGEQHYSGCSFSGHDHVDGGTSRFDPRGNIVQSVCASCGGCQ
                     QFPTYPNPGAWSNTNHSTNCNNAIFKIHLKSDIATANFIIPQSGCAPYEVQFQNTSVG
                     LSFLWDFGDTISGAANYSSLKNPSHTYHQAGLYTITLISFLPGSCNSSDTIQKNLLIL
                     NDTTMWLDSLVICPNNSVQIGISPSPDTNVCYQWIPDTCLSNPAISNPFAFPQTTTTY
                     MCLVTHPGCTDTLLQTLTVIPFIISAGEDTVSCSKWFTLQGSSESAGTLFHWSSLKNF
                     LDTLNLSPFDSTAFIDLTSYSGNTFYLKAYNSIGCESIDSVKVIYGGTSISTSSDRLA
                     CYNDTLHLHAYNLYPNNILTWAWQPEAYILTGAHSPDPIVKPDTSTWFFVS"
BASE COUNT         3054 a         2010 c         2021 g         3002 t
ORIGIN      
        1 caaaggttct atctcaagca attccttttc gatgtaatcg aaaagatctt tacggctaat
       61 ttgaggtggg aagaatgagc ctacagggtc tttttcggtc acaaaaggaa ctgtcccaaa
      121 catatcgaga gcatgataat agctcaaagc acgcataaac cgggcttcag cacgataatg
      181 ttcgatatcg gtacgaaggc tgccagtaac attgcgttca tcgagtttag catcggttgt
      241 ttctctgata aattcattac aaagagaaat ttgatagaat atacgatagt agaaggcgga
      301 gataaaaaca tcgccggcac cccatgtctg ccaatggaaa tcctttattg tctggtcgtt
      361 ccatccaata atggcttcat cggtagtgag ttcctggtga taccaatacc cacgcagata
      421 ttggccaaat ccttcgtcaa taccactaat atcgggcatg cccgaagggc cctgttgacc
      481 ggagacagcc aagccggcat aaattttagc aagaatctgc ttgtaggctt taggattttc
      541 atatacctgg gctgaagtaa caacatcctt gtcaattgga actgtatcga ggtccttggt
      601 gcaggaggcc aaaacaaagc tcatacctgc aaaaattaaa aagaatttac tgaacttatt
      661 catattagct gtttttttat cggttaaaat attaaattaa gacccagaac gaataccctg
      721 gtacgaggat acatgctata gtcgatcccg ctgaatattt caggatcgag acctttgtaa
      781 ttggtaatgg taaacacgtt atttaccgta gccgaaagac gcagtttagc gctgctgttg
      841 aacaatttat cgaaagtata gcctatggct aaattatcca atttgaagaa ggaagcattt
      901 ttgatgtaat aatcggagag atattgagga actttaaagc ctgtttcggc aacatcggca
      961 cttacattgg ccagataagg tccctcgggg cggtatagac gttcataggt agaattatta
     1021 gagctgaagt tatcatatac atagttgcct atattagccc gaccgctaaa tatgaaatcc
     1081 cagttcttgt attgcatacg gctgttgatg cccagataga catcggctgc aggtttttta
     1141 taatgatatc tatcatcatc gttgattttt ccatctttat tgcggtcaac ataaagccct
     1201 tcgatgggtt ttccattggc atcgtaaacc tgttcataaa cgaagaaact gttagaaggg
     1261 taacctaccg aatgaatttg gatggtattg ccaacgccac ctgcaatggt acctacaagt
     1321 actcctaaat agttaggatc gtcacttgct gttagtttgg taattttatt tttgttatag
     1381 gtagcattga cacccaaatc ccagtagaaa tctggaccaa caaaaggttt cccattgatg
     1441 gcaaactcca ctcctttatt ttcgaggtcg cctatattgg tggtgatgta gttggaaaga
     1501 ttagaacctg ccggcacagg aatgaagttg ataagatctt tggtttttct ccaataaaca
     1561 tccagcgaac catagattct atcgttaagg aaaccataat cgagtccgat attggtagtt
     1621 gtggtttctt cccatttgat attcgggtca taaccttcgg gacgcaaggt gatcaacgta
     1681 tctgtcccca gaatgtaaga agctgtagcc caagaactcg tgtaacgagg taaatatgga
     1741 tagtcatcat tggtaatgtt ttgttgacct gtgatacccc aacccaaacg aagcttcaat
     1801 tgggataggg ttttcatatt tttcatccaa ggttcatcca tgattctcca ggctaaggca
     1861 accgaaggga acaatcccca gcgattatcc ttcgaaaatc tcgaagaacc atcatcacgc
     1921 aacgtaaagg taagtaggta cctatcgaac aaggaataat tgaacctcgt aaagaaagaa
     1981 accaaataaa attcactttt atttgaactt gtatcgaaaa ttctcttttg gataaagaaa
     2041 ggtccattca aatcgttggt cgttgaattc gtatcctcac gatagaaatg ctgccatgaa
     2101 taaccaccca tgacatcgaa tttgctaaat atggtgggaa cttctttgac gtaatttaaa
     2161 gtaaaatcga gcagttcatt acgtttttcc tgagtatatt gtgtgttagt gccacctcca
     2221 tattgagggt cgaaagcaaa agcggcattt cctgggacat aaactgttcc attggtttta
     2281 gaataatcga gaccaaggtt aaggttagct ctcaattcag gaaggaaggg cattttataa
     2341 tcgaactggg cgtttcctaa gaatcggctc acatcagcat tatcgtgttt ttgttcaagc
     2401 agtgccactg gattatcggt cgcttgatcc acaggatttc cattggcttg aagccaggtc
     2461 cagtatccac cataagcgtt cgagtcgtga actggtttgg tgggatccat ctgaatggcc
     2521 gacccaatag catcttcatt ggcaaaacgc tggtcgatga acattccctt tccgttgagg
     2581 ttaattttta ggttgtcatc gaaaagagaa ggattaaggt tgagtccaaa ggtcgttctt
     2641 tgcatattgt cggtcttaag aatgccatca taattggcat agcccacgga agcacgatag
     2701 ggcaatgcgc caatggcacc tgttgcactt accgtatgat cgtgcgcaaa tgatgaacgg
     2761 taaatttcct cttgccaatc ggtattggca tctcccagca aagcaagaac ttcaggtctg
     2821 ttggcatagc gttcattgat gatgctcctg aattgatcac catcaaaaac ttcacgtttt
     2881 ttagggatgg tgtagaggga aaaacctccg ttatattcga attgaggagc ttttttgcca
     2941 ggagctaaag tacctttctt ggtagtaatg attataactc cattggatgc acgattacca
     3001 tagatagctg ctgccgaagc atccttcagg atggtaaagg tttcgatgtc gttaggattg
     3061 atggtactca atggatcgcg tgttccataa atggaagtat tgtcaatagg cacaccatcg
     3121 ataacaaaaa gaggatcgtt gcttgccgac aatgaagaac ctccacggat acggatagta
     3181 gctccagcac ctggcgcacc actattggta acaatctgca caccggccgt tttaccagcc
     3241 atcaattcaa ttgggttggt aatacttcca cggttaaaat cctgcgttgt cactgcagta
     3301 atcgaacccg tagcatctcc ttttttcacc tgtccatatc ctataaccaa tatctggcta
     3361 agactggtga tggttggaga tagagctaca ttaagggttt gtgaggtttc aatcacaaac
     3421 tcctgagttt gatagcctac aaatgaaaaa gcaagagtag atcccttagg tacggtaata
     3481 ctgtaattac cgttgatgtc agtaactgta cctgtagtag tgtttttcac tacaatggta
     3541 actcctggta gcgtggttcc atcagaagca tccgttactt tcccctttac tgtaagctgc
     3601 tgcgcaaacg aaggcagagc aacagccgca aaaaagaaaa gaaacagtac cttcagtaag
     3661 ttagcatgtt tcatcataca tctggtttta gttatggttt aacattaaat gatttggtta
     3721 acacctcttt tcagaatatt ctttcatttt cgatgaaaaa actactgaaa acagatggtt
     3781 cgattttaac tttttcccga accaaatata caactttttt cgttttcaat tgcaaatatg
     3841 tttcactgat tttgagattt ttgagttttt ttttgcgaaa acgatttcgc aaacgtttgc
     3901 gcttgaaaaa ttattgaatt attttcgttg gccgcgtata aatcgttcga taaatcatcg
     3961 aaggaagcgg cataaaatta ttaagcaatt aaaagtcagg gtattatgca aaataaagtt
     4021 actatcaaag atatagccaa aacattggga gtttctatat caacggtatc cagggcactg
     4081 aaagatcatc ccgatatcag tgaatcgact agaaatctca ttaagaattt tgcgcaggaa
     4141 atcaactatc gccctaatgt tttggctttg agtctcaggc gacaaaaaag caataccatt
     4201 ggactgatca ttcccgaaat cgttcatcat tttttttcat cggtgattag tggcatggaa
     4261 gacctcagct atcgtgaagg atacaatatc attatttgcc aatcgacaga aaatcagttt
     4321 cgagaagcta cagcacttca aactttgatt gatcacaggg tggatggaat attggtttca
     4381 ctgagcaaat ccacacaaaa tttttctcaa ttcaagaagg cacaggaaga aggtttcccc
     4441 atggtatttt tcgatagaat ttgcgaaagt atagaaaccg atcgagtagt catggatgat
     4501 ctagaaggag gtcgcttggc aaccgaatac ctgatcaata aaggctgcca aaatatactt
     4561 catctggcgg catcgcccat tttactgatt ggacgtaacc gccgcgatgg atacctccaa
     4621 gcattgaaag atcacaatat cccaatcaaa gaagaactta tcctgaaatg tgatactcag
     4681 gaagaagttt caacccttag aaaccatctt tatgaacttt taccgcaaat tgatggggtt
     4741 tttgcggtaa atgacagtac tgccatcgcc atgattcaaa tactgaaaaa aaaaggctat
     4801 cgaattcctc aagatattaa agtagtaggt tttggcgatg gacctaatgc tactatagta
     4861 gaacccgccc tcaccacaat agaacaaaaa ggttacgaca tgggccgtga atccgtaaga
     4921 ctgctcctgc agcgcctcaa caatccatcg gcttccatca gttatcaaac ccgtgttttc
     4981 cctcctacac tcaagatcag agagtctgcc tgatgaattc tttgcgccca taattctcga
     5041 cagaatagcc ccttaaaaat aaaacaaaac aatgaataaa agactgaata tgaaataatt
     5101 aagcaagatg aatcattaga gcttgcattc tatcatttca tagaacgaag gatttgtgat
     5161 aaaatcaatg gaaggtggat gcctgaaaaa tttctttttt ttacctatga tttctcacat
     5221 aaattgttca ttcaaccatt attgaaaatt tctacataaa aaaattcttg ttttctttga
     5281 aaaaagtgat ttttatgtat acctttgtta ataaatatca aacctgattc aaaaaccatt
     5341 ctattgtatt ctttcagttt aacatcttat aaattacttt gtgatttctc atgtattaca
     5401 tctatcttac atcattcata attaaaagtc atcgaaccac atttttaaaa tcaaccttaa
     5461 gtttaatcca aaatccttaa caactatgaa acatttattt cctttgctca gtttagtttt
     5521 acttgttagc ttattcaaca taggctgcag taaagatgaa gatgaagaat tacccaaaga
     5581 tctggtagtt aattacactg ccagttttac cgacgtagct tctacactgc aagattttat
     5641 catgaaagca caagtgtatg aaagttctaa ttttgaatca ttaactgccg atcaaggcaa
     5701 gaagatcatc aacgattaca ttacagctgg tgaaaatttc atcaagagtg tggatgatta
     5761 tgaaaaatac aagattggca ccaaaaattc ttccccatgg ttaaagaatt cgatggattc
     5821 accctgtagc atgtacgatg ctatccctga tgtggggaat ggaatatcac caggattcgt
     5881 aaaaagtgtt ggtgatttga tagctgacac aaaaggcgat gtaaaaaaga ttgagaaaaa
     5941 atttaacgat ggcgagataa gtgaagaagt ttttacggcg gctctcgatt cgttaaagaa
     6001 aataaaaaca ttgaaggcag cgaattttgg gatgagctcc attattggag ctggaacatc
     6061 gggtttaaca ggggcagcta tttttagtgt agggggaatc ggtatggcca tgtctgcgcc
     6121 tgccctgctg ggagtaacag ccgcaggagt tgttgttggt acgggatatt atttgatatc
     6181 caattggtgg tcaggaatta agaaaaatgg acaggatgac aaattaggcg agattgtagt
     6241 ggcttcagga aaaattgggg atcctattcc tacaactatg ctcagtaatg gcgccagtct
     6301 aactgttgct atttcaggtt acgctccggt ttatattcca gcattcatcc taccttcgca
     6361 aggaataaag aaaaccctcg aaattactcc gattaaatta gaagacgcca ctcctacaac
     6421 aaaggtagaa gtatgcaccc tagaagaaga atttacatct gaaaactgtg aagatgtact
     6481 ttatgttact gccacaccct cacctatcga cccaggccca ggcgaaggcg ttacagtaac
     6541 tgccaccatt ttccctccca tagcaggatg ccaaatttca ttcagtattg ttggaacaga
     6601 tggatacact aaaagcgaaa cgcatgaaac cgatgctaac ggaaaagcat ctttcttcat
     6661 ccctggtggc gccgaaggag tttttgatat cgtgaccatt actacttcga atggccatac
     6721 ttatactgta acttatacat tttaaaatta tctaattgtc ttattataaa aaagccttcg
     6781 ttgccgaagg cttttttatt gccttgttca cttgttcaat tatttaactt atatttgttt
     6841 ctctatgaaa acaaggcatt tattaagaaa gacatttatt attgggctgg tggtttttag
     6901 tcttcccaga ttatattccc aaaatggctt cactgagaat aaaggacaat gggattctaa
     6961 cgttctcttc ttcttgcaac cccaagcaga tgtcgcagta tttattgaag caggtgccat
     7021 aaccttcaat attcaactat tacggcatga cgaaacacca aattttgaaa atattagcgc
     7081 taaaactaat cacgaaggtg ttaaaggaca tgcatttcga attattttcg aagatgctga
     7141 tttttcaaat atcactgctt taagaccaaa acccgaacta ctcaattatt ttcttggaaa
     7201 aaatcccgaa aaatggagta ccggtgtaaa aatctttaat gaattataca tctatgatgc
     7261 atacaagaat atagacctta aaattatttc cgatattgga ggtattaaat acgaattcat
     7321 cattcatcga ggagggcggg tcaaagatat tgccattcgg tatgatggat tggagaatat
     7381 atcattgagc aatggccaac ttattttgag tacatcattg ggagaaatta tcgaagaagc
     7441 tccgaaagtc atagttgaag acaaaggaag cacatttgtg actgggtgtc aatatcaact
     7501 cgaagccaac aggataaaat ttcatttaga tccatcattc ctcaactctg acaaaattgt
     7561 tattgatccc cgtttggtat tttccacgta ttcaggaagc gaggccgaca attggggttt
     7621 tactggtact tacgacgagg cagggaatgt atattccgga ggaattgttt tcaatattgg
     7681 atatccagta agtttaggag cttatcaaca aagttttggt ggtgggtgcg acataggcat
     7741 tatcaaatac gattctacgg gaacaaatcg catctatgct acttatttgg gtggacttta
     7801 tgccgatctg ccgcatagtt taatcgtcaa tacatccaac gaactggtta tttttggtac
     7861 cactggttcg cccaactttc cagttacgcc atcggcttac gatacaagtt tcaacggagg
     7921 ggatgctgtt aattatgcat ctttaaattt tcctttaggc tccgatattt ttattgctaa
     7981 attcagtgaa gacggctcac aattaatagc ttctactttt ataggaggca gcgaaaacga
     8041 tggcttaaat tttcgctcac gctatgcatc catgcttatg acaggcaacg atactttata
     8101 ctataattat ggagatggtg cccgtggcga agtaatcacc gatccgcaag ataacattta
     8161 tattggctcc accacatttt cgaacgattt tccgataact gccgcaactt ttcaaccctc
     8221 ttataaaggg aaacaggaag gtgtggtatg taaatttgac aggacattga gctatttgaa
     8281 ctggagcagt tatttaggcg gatcgaacga tgaaggtata ttttccatcg atatcaactc
     8341 ttatggcgat ttatacgtta ctggtggcac caccagcagc gatttccccg taacatctgg
     8401 agcctatcga acccaattta atggaggaag tgccgatgcg tttatttcgt gcatttcggc
     8461 taatggagta aatttaaagg gatcgacctt ttggggatcg ctatcttatg atcagggata
     8521 ttttgtgcgt atcgggcccg atgatgaagt atatgtattc gggcaaacta aagcctctga
     8581 ttcgtccatg atgatcgatg ccctttatgg ccagccaaac agtggacaat tcatatcgaa
     8641 attttcacct ctactcgatg aactgagatg gagtacagta gttggaaaag gcgatggcca
     8701 acccaatatt tctccaacgg catttatggt cgatgtatgt gggaggattt attattcggg
     8761 atggggaaga atatggggta acagttatgt aaatggtaac tggtatttat ggggtacccc
     8821 ttttggaact cacggccttg cggttactcc cgatgctgtt caacccatca ccgatggaca
     8881 agatttttat atagccatat tatcatccga agcggcagga ttagattatg ctactttttt
     8941 tggagaacag cactattcgg gttgttcatt cagtggtcat gatcatgtgg atggaggcac
     9001 aagccgcttc gatccgagag gaaacatcgt tcaatcggtt tgtgcctcat gcggaggctg
     9061 tcagcaattc ccaacttatc ccaatcctgg tgcatggtcg aatacaaacc attcaaccaa
     9121 ctgcaacaat gccatattta agattcattt gaaaagtgat attgccacag ccaatttcat
     9181 catccctcaa agtggttgcg ctccatacga agttcaattc caaaatacca gcgttggttt
     9241 gtcttttttg tgggattttg gagatacgat ctcgggagcc gccaattact ccagtttgaa
     9301 aaatccatct catacttatc accaagccgg attatacact attaccctca taagcttttt
     9361 accaggcagt tgcaattcat cggatacgat tcagaaaaac ttgcttattc taaacgatac
     9421 tacaatgtgg ctcgatagct tggtgatttg cccgaataat tctgttcaga ttggaatttc
     9481 tccttcccca gatacgaatg tatgctatca atggattcct gatacttgcc tcagtaaccc
     9541 tgctatttcc aatcctttcg cattcccgca aactaccaca acttacatgt gcttggttac
     9601 acatcccgga tgcaccgata ccctccttca aaccctcaca gtgattccat tcattatttc
     9661 ggcaggcgaa gataccgtaa gttgttccaa atggtttacc ctacaaggtt catcggaaag
     9721 tgcaggtact ctattccatt ggtcatcttt aaaaaatttt ctcgatacat tgaacttatc
     9781 tccctttgat agcactgctt tcatcgatct aaccagctac tcaggaaata cattttatct
     9841 aaaagcttat aattcaattg gatgtgaatc tatcgatagt gtaaaagtaa tttatggagg
     9901 gacaagcatt tcgaccagtt cagaccgttt agcttgctat aacgacactt tacatctaca
     9961 tgcctataat ttatatccta acaatatatt aacatgggca tggcaacccg aagcctatat
    10021 tcttacaggc gctcacagtc cggaccccat agtcaagccc gatacaagca catggttctt
    10081 tgtgagt
//
LOCUS       DAOORU010000087        10035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8346_length_10035_cov_5.232064, whole genome shotgun sequence.
ACCESSION   DAOORU010000087 DAOORU010000000
VERSION     DAOORU010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10035
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8346_length_10035_cov_5.232064"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..1119)
                     /locus_tag="PLF20_06540"
                     /pseudo
     CDS             complement(31..1119)
                     /locus_tag="PLF20_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339297.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
     gene            1333..2694
                     /locus_tag="PLF20_06545"
     CDS             1333..2694
                     /locus_tag="PLF20_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOX80696.1"
                     /translation="MNDLIMSAFKAYDIRGIFNEDFDEEDVFRIGYYLPGLLRTHKIL
                     IGSDARESSPAIHKALCDGVIRAGADVYDMGMSTTPMVYWATARFGFGASIMITASHN
                     PPQYNGMKISRENALPVGYDSGLNKLEHLIQSPVVHNAVINGKIIEFDKKTEYLDFLI
                     HSGQYDFGNLKMAIDCGNGMAGLFARGLFGNESFYLFEDIDGRFPNHLPNPLEGKNLE
                     PLKAKVLERECDLGVIFDGDADRVMFIDENAQFISPDLMIAVLAHYYLRNDRLYARVL
                     HDIRTSKSVSEYIEKLGGQVHIWKVGRAFAAPKLREIDGLFGGELAGHYYFKDFYFSD
                     SGLMSALRLLTVLSRFKTRGVRPSRLIQSIQHYHNSGELNFLVRNKTEAMNELLRVFL
                     SKEDPLRVYDFDGYRVEFSGEWFNVRPSNTEPYLRFIAEANTSEKLSEMIETTKAILA
                     RFM"
     gene            complement(2957..3029)
                     /locus_tag="PLF20_06550"
     tRNA            complement(2957..3029)
                     /locus_tag="PLF20_06550"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2994..2996),aa:Thr,seq:tgt)
     gene            3215..4087
                     /locus_tag="PLF20_06555"
     CDS             3215..4087
                     /locus_tag="PLF20_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010539018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOX80697.1"
                     /translation="MSFRIIVLAKQVPDTRNVGKDAMKADGTVNRAALPAIFNPDDLN
                     ALEMALKIKDQMVDAEVIIVTMGPSRAAEIIREALYRGADRGYLVTDRKFAGSDTLAT
                     SYTLSLAIRKLEPFHLLITGRQAIDGDTAQVGPQTAEKLGIPQITYVEELISIDLQQV
                     VVKRRLEQGVETVKSPLPVCITVHGSAPACRPRNAKLLMKFKHARTYTELQEETRDYT
                     QLRFEKPYLDIEELSGDALEADEEKIGLAGSPTKVKKIENVVFQHKESKILNNSDQDI
                     EWLVRQLIQNHIIG"
     gene            4118..5140
                     /locus_tag="PLF20_06560"
     CDS             4118..5140
                     /locus_tag="PLF20_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007486135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOX80698.1"
                     /translation="MNNVFVYCEINEEGHIAEVSLELLSKGRRLANKLNVELEALAIG
                     YRLENIADEVFTYGVNILHLADDKRLFPYLTLPHASIVTGIFKEEQPQIALFGATSIG
                     RDLAPRVASALRCGLTADCTSLEIGNHTELKSNKVYQNLLYQIRPAFGGNIIATIVNP
                     DTRPQMATVRDGVMKKEPLPSPFSGKINHVEVQKYLSDVDFVVQIIDRHIEQRKVNLK
                     GAQIIVSGGYGVGTPENFKLLYELAETIGAEVGASRAAVDAGLADHERQIGQTGVTVQ
                     PKLDIACGISGAIQHGAGMDKSAQIISINKDPNAPINQIADYTIIGDVAEIIPKFIHF
                     YKLNSK"
     gene            5154..6857
                     /locus_tag="PLF20_06565"
     CDS             5154..6857
                     /locus_tag="PLF20_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOX80699.1"
                     /translation="MANFYTDNKSLKFYLSHPLMPKIISLREFDYTEKNEYDYAPIDF
                     EDALDSYDKVLEIIGEISGDIIAVNADDVDKEGPHLVNNEVVYARGTQENLEAIKNAG
                     LFGISLPRKYGGLNFSIVPYVMSAEIVSRADASFSNIWGLQDCAETIHEFASEEIKNE
                     FLPRFPRGATAAMALTEPDAGSDLQAVQLKATPNQDGKTWRLNGVKRFITNGDADISL
                     VLARSEENTTDARGLSLFLYDRNSKAVTIRRIENKLGIKGSPTCELVFRDAPALLVGE
                     RKLGLIKYVIALMNSARLGVGAQSVGIAEAAYREALKYANEREQFGKAIVNYPAIYEM
                     LIRMKVKIDAMRSLLYETARFVDINKSYNIAAEKRKLEPNERNEQKYYQKMADMYTPL
                     LKLISSEYCNQIVYDALQIHGGPGFMKDFPVERLYRDARITSIYEGTSQLQVVAAIRG
                     VTTGFYLKAIQDYDSQIIIPEEYLKKSLQLMTQQYKETVDKVNTEKDSEIVEFHSRRL
                     VEMAGNIILGYLLLLDSQRNPEFLKSARIFIENAKSENQSMHEYIMNFNDREIDNFKF
                     I"
     gene            complement(7157..7978)
                     /locus_tag="PLF20_06570"
     CDS             complement(7157..7978)
                     /locus_tag="PLF20_06570"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOX80700.1"
                     /translation="MRKIYILSDGTGRTASYALEAAMVQFQEMKDETKIELFPDVRTS
                     QQIDQIVEKASKEEALIIYSLVGEQTRHYMQTICRHYIIETVDLMGPLLMKLSAHFER
                     QPAGTPGLYQQMNKEYFRRIDAVQFAFTHDDGLRIEELSKAEIVLVGVSRSFKTPLSI
                     YLAYKGWFVANVPIVLDIEPPEELFRIPPHRVFALTTSALELSKLRQTRVEILNGYAE
                     NYASFDYVKKELAWAHTIYRKQPLWHIIHISHKSIEEISIEILSFIRQHHNNHPF"
     gene            complement(8102..8557)
                     /gene="smpB"
                     /locus_tag="PLF20_06575"
     CDS             complement(8102..8557)
                     /gene="smpB"
                     /locus_tag="PLF20_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOX80701.1"
                     /translation="MRRKSDINIKNRKAGFEYFLIETYVAGIQLTGTEIKSIRAGKVN
                     LTDAYCSFIGNELFVLNMHISEYELGTHYNHDPKRHRKLLLTKRELKRLQTKVKEKGF
                     TIIPIRLFINEKGYAKLEIALAKGKHSYDKRESIKQKDQKRDMERFENY"
     gene            8793..9737
                     /gene="arcC"
                     /locus_tag="PLF20_06580"
     CDS             8793..9737
                     /gene="arcC"
                     /locus_tag="PLF20_06580"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOX80702.1"
                     /translation="MDKLAVIAFGGNALLRGDQKGTIDEQEANVYETCTYLVPLIKQG
                     YNFVIGHGNGPQVGNVILQHQAGEKMFGIPAQPIDFCVAETQGSIGYMIEQQLRNVLI
                     EEHLERDILTIITEVIVDKDDPAFHNPTKPVGPFYSKEEADKIAAETSAKFHEDPRGR
                     GWRKVVASPMPLQINNVKSIEKLARDGQIIIAVGGGGIPVFWVHSNKLQGIDAVIDKD
                     LASSLLAVEIKADIFIILTDIPKVYINFHKPDEKALDRMSITEARRYLSDNQFSEGSM
                     APKVRACIKFVEKSGKEALITMASELGKPGAGTIIYAE"
     gene            9773..>10035
                     /locus_tag="PLF20_06585"
     CDS             9773..>10035
                     /locus_tag="PLF20_06585"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136980.1"
                     /note="catalyzes the formation of ornithine and
                     carbamylphosphate from citrulline in the arginine
                     catabolic pathway; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase subunit F"
                     /protein_id="HOX80703.1"
                     /translation="MAFNLRNRSFLKLLDFTSEEIKYLLGLSKELKKAKYTGTEHQRL
                     KGKNIVLLFEKDSTRTRCAFEVAALDQGAHVTYIGPSGSQMGK"
BASE COUNT         2945 a         1946 c         2161 g         2983 t
ORIGIN      
        1 ggcgcgaaaa agctagatgc cagcgcgata caagaaccac tgctgcgaaa gtcgtggctc
       61 gatcattgca tctgttcttt cgctgaatcc gactcggttc aaataaggtt cttctttcac
      121 aagaaatcct ttttggcgta agtcttcagt aattttatta cgaaccttca aacggtcttc
      181 acccacataa agaatggcct tttcgttcaa agttccatcg tcattgaaga tatcaatggc
      241 atcgagctta tgtttcatac ctaaattata gtcgttgatg tcgtgagcag gagtaacctt
      301 cagacaaccc gtaccaaatt ctctgtcgat gtattcatcg gtgataatgg gtactattct
      361 gttgatgagc ggtataactg cttttcggcc gtgaagatgt ttgtatcttt catcatcggg
      421 atgaatgcag actgctgtat cgcccaaaat ggtctcgggt cgggtcgtgg caatgatgat
      481 ccattcgtct tcactatcgt aaattttata tctcacataa tagagtttgg attgcacttc
      541 cttgtaaatg acttcttcgt cggacaaggc cgttaatgct tgtggatccc agttaatcat
      601 ccttacgcct ttgtagatga gtcctttatg gtataaatcg acgaaaacct cgattaccga
      661 ctcgtacata tcgggatcca tggtaaattt cgttctcgac cagtcgcacg aagctcctaa
      721 tttacgaagc tgctgcagaa tgatacctcc atgtttttct ttccattccc aagcatattt
      781 aagaaactcc tcacgggaaa gtgaagattt ttcgattcct ttttctcgca ggttagctac
      841 cactttggct tcggtagcta ttgatgcatg gtcggtacca ggcacccaga gtgtattttt
      901 cccctgcatg cgagccctgc gtattagtac atcctgaatt gtattgttga gcatgtgccc
      961 catgtgcaat actccggtaa cattcggagg aggtatgaca atacaataag gttcacgctc
     1021 gtcgggcacc gaatggaaaa aaccatgact ttcccagaaa gaataccatt tatcttcggt
     1081 agtttcagga ttgtatctcg aaggaatctc ttttgtcatt tgaaggatgt tatgactcat
     1141 tacggccgca aaattaagaa aacactatca atctctttgt taggatatac agcctcgatg
     1201 atggaaagcc ccatttgcct tagtgtatcc aacctgttga ttccagaaat acctcaaact
     1261 gaaaaatgag aaggcatcat tgaaccgaga gaatgaagtt ggtgtaaata attacttttg
     1321 tatcaaccac aaatgaatga tttgattatg tcggctttca aggcttatga tatcagaggc
     1381 atattcaacg aagatttcga tgaggaagat gttttccgca taggttatta tttgcctggt
     1441 ttgcttcgaa cccacaaaat attgattgga agtgatgcca gagaatcatc tccggctatt
     1501 cacaaggcat tgtgtgatgg agtcatacga gcgggtgccg atgtttatga tatggggatg
     1561 agcacaactc ctatggttta ttgggcaaca gctcgtttcg gttttggggc ttccatcatg
     1621 ataacggctt cgcataaccc ccctcagtat aatgggatga agatcagtag agaaaatgct
     1681 ttgcctgtag gatatgattc tggattgaac aaacttgaac acttgataca atcgcccgtt
     1741 gtgcataatg ctgttataaa tgggaaaatc atcgaattcg acaagaagac agaatatctt
     1801 gattttctga ttcattcagg gcaatatgac tttggaaatc taaagatggc tatagattgc
     1861 gggaatggca tggcgggttt gtttgccaga ggattgttcg gaaatgaatc gttttacttg
     1921 ttcgaagata tcgacggacg ttttcccaat catcttccca atcctctgga gggaaaaaat
     1981 ttggaacctt tgaaggcaaa agtgctcgaa agggaatgcg atttgggagt tattttcgat
     2041 ggagacgccg accgtgtgat gtttatagat gaaaatgcgc aatttatttc gcctgatcta
     2101 atgattgccg tgttagcgca ttattatcta agaaacgatc gattgtatgc cagggttctg
     2161 catgatattc gaacttcgaa atctgtatcg gaatatatcg aaaagttagg agggcaggtt
     2221 catatctgga aggttggcag ggcctttgct gctccaaagt taagagagat agatggtttg
     2281 tttggaggtg aactggctgg ccattattat ttcaaagatt tttatttttc cgattctggc
     2341 ttgatgagcg cattacgatt attgaccgtt ctttccagat tcaaaacccg gggtgttcgt
     2401 ccttctcgac tcattcagtc cattcaacat tatcataatt caggagaatt gaattttctt
     2461 gttcgaaata aaactgaagc catgaacgag cttcttcgtg tatttctttc gaaagaagat
     2521 cctctccgag tttatgattt cgatgggtat cgggttgaat tctcaggtga atggttcaat
     2581 gtaagaccct ctaatacaga accttatttg cgttttattg ctgaagccaa tacctctgag
     2641 aaattatcgg aaatgattga aacgacaaaa gctatacttg cgagatttat gtgaggaatt
     2701 tattcgaatg attttattat aaatatttgt aataggcgag ctaatccctt cggatggttt
     2761 gagccaaata gtcatcaaat tttgccttca ggcttattac aaggttatac gggagaattt
     2821 aacctgttct gacaatgatc tgatattttt ccccttcttg atgagattat cgcctatagc
     2881 atgtattaaa aaatccatta ttctggcaaa aagaatgaca aaacgatgga tgtacttctc
     2941 aaatcaccaa taaatgagcc gatggacgga ctcgaaccgc cgaccggctg attacaaatc
     3001 agctgctcta ccaactgagc tacatcggct gaaaatcgtt gcaaaattat aattttttca
     3061 caaagaatca tccattctga aaaataaccc tattttctat tcttatctga tgaaaaatgc
     3121 cagaattttt gccctgaggt ttttttgtaa ttttgcggct tccttttgtg aagtttttat
     3181 aacttgagga aagaaaacct aattgtcaga tattatgtcg tttagaatta tcgttcttgc
     3241 caaacaggta cccgataccc gtaatgtagg caaagatgct atgaaagctg atggaaccgt
     3301 caacagggcg gcacttccag ctattttcaa tcccgatgat ttgaatgcac tcgaaatggc
     3361 ccttaagatc aaagatcaga tggtggatgc cgaagtgatt atcgtaacca tgggtccatc
     3421 tcgagcggcc gaaattattc gtgaagcgct ttaccgcggt gccgaccgag gatatctggt
     3481 aaccgatcgc aaatttgccg gcagcgatac tctggctact tcctatacac tttcgcttgc
     3541 catcagaaaa ctcgaacctt ttcatcttct cattacgggc cggcaggcca tcgatgggga
     3601 taccgcacaa gtgggtcctc aaaccgccga aaaacttggc atacctcaaa ttacttatgt
     3661 tgaagaactg atttctatcg atttacagca agttgtcgta aaacgcagat tagagcaggg
     3721 agtcgaaaca gtaaagtcgc ctttgccggt ttgcattacg gtacatggct cagcacctgc
     3781 ctgccgacca cggaatgcca aattgctgat gaagttcaaa catgctcgta catataccga
     3841 acttcaggaa gaaacccgcg actataccca attgcgcttc gaaaagcctt atctcgatat
     3901 cgaagaattg tcgggcgatg ctcttgaagc cgatgaagaa aaaataggac tggcaggttc
     3961 acctaccaaa gtaaagaaaa tcgaaaatgt cgtatttcag cataaagaat caaaaattct
     4021 gaacaacagc gatcaggata tagaatggtt agtacgacag ctgattcaga accatattat
     4081 tggataatgt ttgccttaca atttaaaaga aagtcttgtg aataacgtat ttgtatattg
     4141 cgaaatcaac gaagaaggcc atatagccga agtgagcctc gaactgttat cgaaaggccg
     4201 taggctggcc aacaaactga atgtcgaatt ggaagccctg gctataggat atcgactcga
     4261 aaatattgca gatgaggttt ttacttatgg agtcaatatc cttcatttag ccgacgataa
     4321 aaggctattt ccatatttga cccttccgca tgcttctatt gtaactggaa ttttcaaaga
     4381 agaacagcca cagattgcgt tatttggtgc tacctccatt ggccgcgacc ttgcaccacg
     4441 cgtggcctct gcattgagat gcggacttac tgcggattgt acgagccttg aaataggcaa
     4501 ccatactgaa ctaaaaagca ataaggtata tcagaatctt ctctaccaga ttcggccggc
     4561 ttttgggggt aatattattg caacaattgt taaccctgat acacggcctc aaatggccac
     4621 tgttcgtgat ggagtcatga agaaggaacc tcttccatcg ccattctctg gaaaaataaa
     4681 tcatgtggag gtacaaaagt atttatccga tgtcgacttt gttgtccaga tcattgatcg
     4741 tcatatcgaa caacgaaagg tgaatctgaa gggtgctcaa atcattgttt cgggtggcta
     4801 tggagtgggt acgcccgaaa attttaagtt gctttatgaa ttagccgaga ccattggagc
     4861 tgaggtaggt gcttcccgtg ctgctgtgga tgccggactg gccgatcatg aacgccagat
     4921 tggacaaacc ggtgttactg ttcaacctaa attggatata gcctgtggaa tatcgggcgc
     4981 aattcagcac ggagcaggca tggataaaag cgctcaaatc atttctatca ataaggatcc
     5041 caatgcccct atcaatcaaa ttgccgatta taccatcata ggcgatgtgg ctgaaatcat
     5101 tcctaaattt atccattttt ataaactcaa ctcgaagtaa taattaatat atcatggcaa
     5161 atttttatac cgacaacaag tcccttaaat tttatctttc ccatcctttg atgccgaaaa
     5221 tcattagttt aagagaattc gactatactg aaaagaacga gtatgattat gcccctattg
     5281 atttcgaaga tgcgctcgat agttatgata aggtgctgga gattattggg gagattagtg
     5341 gcgatattat tgcagtgaat gccgatgatg tggataaaga aggtccgcat cttgtgaata
     5401 atgaagtagt atatgcaaga ggaactcagg aaaatttgga agctatcaaa aatgcaggat
     5461 tatttggaat ttcactgccc cgaaagtatg gtggtttgaa tttttctatt gttccctatg
     5521 tgatgtcggc cgaaatagtt tcacgtgccg atgccagttt ttcgaatata tggggtttac
     5581 aggactgtgc cgaaaccatt catgagtttg cctcagaaga gatcaagaat gagtttctac
     5641 ctcgatttcc tagaggtgct actgcggcta tggctttgac cgaacccgat gccggcagtg
     5701 atcttcaggc agtccagctc aaggcaacgc ccaatcagga tggtaaaact tggcgattga
     5761 atggggtgaa acgctttatt accaatggag atgccgacat atctctggtt ttggctcgct
     5821 cggaagagaa tactaccgat gccagaggcc tttccttgtt tttgtatgat agaaattcga
     5881 aagcagttac cattcgcaga atcgagaaca aacttgggat aaaaggttca cctacctgtg
     5941 agctcgtatt tcgagatgct ccagctttat tggtagggga gaggaaattg ggcctcatca
     6001 aatacgtaat tgccctgatg aattcggctc gtctgggtgt gggagcccaa tctgtaggca
     6061 tagccgaggc agcttaccgc gaagcattga aatatgccaa cgaacgcgaa caatttggca
     6121 aggcaatcgt caattaccca gcaatatacg aaatgcttat acgcatgaag gtaaaaatcg
     6181 atgccatgcg ctccttactt tacgaaaccg cccgatttgt cgatattaac aaatcatata
     6241 acatagctgc cgaaaaaaga aaactcgagc ctaacgagcg caatgagcaa aagtattatc
     6301 agaaaatggc cgatatgtac acacctctat tgaagcttat ttcaagcgaa tactgtaacc
     6361 agatcgttta tgatgctctc cagatacatg gtggacctgg atttatgaag gatttccccg
     6421 tagagcgcct ataccgggat gccagaatta ctagcattta tgaaggaaca tcacagttac
     6481 aggtagtagc cgccattaga ggcgttacaa ctggatttta tcttaaagca atacaagatt
     6541 atgattcgca gataatcatt ccagaagagt atttgaaaaa gtcgcttcaa ttgatgaccc
     6601 aacaatataa agaaacagtt gacaaggtta atactgaaaa agatagtgaa attgttgaat
     6661 ttcactcccg cagattggta gaaatggcag gaaacattat cttgggttat ctccttttgc
     6721 tcgattctca gcgaaatcct gaattcttga aatctgctcg gattttcatc gaaaatgcca
     6781 aatcagaaaa ccaaagcatg catgaatata tcatgaattt taacgatcgc gagatagata
     6841 atttcaaatt catttagcaa aagttttcca gtaagggcaa tatatcttta cagaaacgat
     6901 atgcaatgaa tgtcgcagca aggctgagag ttcagaggtt ctgttccttt gaaaactttg
     6961 ttacgaccga ccactccttt tcatttgtat tatgatttta aaaacggaca ataatatgtt
     7021 gacatttgca aatgtcttgc gatttgtaaa caagtttgat ttcgatactt tgatttattg
     7081 ccgaattttc agtagcttta ctctggggat agcttttgtg atgtaaggga tagaagtttt
     7141 tcagaagagt ctgaagttaa aagggatggt tgttatggtg ttgcctgata aaagacagga
     7201 tttctatcga aatttcttcg atagatttat ggctgatatg aataatatgc cacaagggct
     7261 gtttgcgata gatagtgtgc gcccatgcca attccttttt cacataatcg aacgaggcat
     7321 agttttcggc atatccattt agaatttcca cacgtgtctg acgtaatttg ctcaactcga
     7381 gagcggaagt ggtcaatgcg aaaacacgat gtggaggaat acggaacagt tcttcgggtg
     7441 gttctatatc gagaacgata gggacattgg caacaaacca gcctttgtac gccagataaa
     7501 tactcaaagg ggtcttgaat gagcggctga cgccaacaag cacaatttcg gctttcgaca
     7561 attcctcgat acgcaaaccg tcatcatgcg taaaagcaaa ctgcacagca tcgattcttc
     7621 taaaatattc tttattcatt tgttgataga gtcctggtgt gccagctggc tgacgctcga
     7681 aatgagccga gagtttcata agtagaggcc ccatcagatc aactgtttct atgatataat
     7741 gcctgcagat agtttgcatg taatgtcggg tttgttctcc cacaagacta taaataataa
     7801 gagcttcctc ttttgatgcc ttttccacaa tctggtcgat ctgctgcgaa gtccttacat
     7861 ccggaaataa ttcaatcttt gtctcatctt tcatttcctg aaactgaacc atggcagctt
     7921 ccaaggcata actcgccgtg cggccagtcc catccgacaa gatgtatatc tttctcatag
     7981 aggtctaata ttattggttt atccttaaat atccgataat tttcgagatt tttaccgatt
     8041 caaagcagag aaattagatt ttttgagaat gggtattgtg tttagagaaa aacaatacaa
     8101 atcaataatt ctcgaagcgt tccatatcgc gtttctggtc tttttgttta atgctttctc
     8161 gtttgtcata gctatgtttt cccttggcca gagcaatttc gagttttgca taaccttttt
     8221 cattgatgaa taaacgtatc ggaatgatgg taaaaccttt ctcttttacc ttggtttgca
     8281 agcgtttgag ttctcttttt gtcagcaata attttctatg gcgtttagga tcgtgattat
     8341 aatgtgtacc taattcatat tcggaaatgt gcatgttcag aacaaataat tcatttccta
     8401 tgaagctaca ataagcatcg gtgagattga ccttgccagc tctgatggat ttgatttccg
     8461 ttcctgtaag ctgtatacca gccacatagg tttctatcag gaaatattcg aatccagctt
     8521 ttcggttttt aatatttatg tcggattttc ttctcattcc ttgcaaaatt atccaaaatg
     8581 ccgttattac gttttttcca gaaataaaaa gcaaagagga attgtcgaac taatttttct
     8641 tcatagattt gcagtcgatt tttaaaatcg attcattagc tgtaagcatt gagcaaggac
     8701 aaaatttcgg cccttgtttt tttgttttca gcatcgagta caatagcaaa tcatcagtta
     8761 ttgttcaatt tattttgcga aaaagtcatt ttatggataa acttgcagta atagcctttg
     8821 gaggcaatgc tcttctacga ggagatcaaa agggaaccat tgatgagcag gaagccaatg
     8881 tatatgaaac ctgtacatat ttagtgcctc tcatcaagca gggatataat tttgtgattg
     8941 ggcatggcaa tgggccgcaa gtggggaatg ttattcttca acatcaagcg ggagaaaaga
     9001 tgtttggtat cccagcccag cccattgatt tttgtgtggc tgaaactcaa ggatctatcg
     9061 gatatatgat agaacagcaa ttacgcaatg ttctcatcga ggagcacctc gagcgagata
     9121 ttttgaccat cattaccgaa gtaattgtag ataaggatga tccggctttt cataacccta
     9181 cgaaacccgt tgggccgttt tattcgaaag aggaggccga taagatagca gccgaaacca
     9241 gtgcaaaatt tcatgaagat cctcggggga gaggctggcg taaggttgta gcttcgccca
     9301 tgcctctgca aatcaacaat gtaaaatcga tcgaaaagct agcacgtgac ggccagatta
     9361 ttattgcggt gggtggagga ggcattccgg ttttttgggt tcattccaat aagcttcaag
     9421 gaatcgatgc cgtaatcgac aaagatctag cttcttcatt actcgctgtt gaaattaaag
     9481 ccgatatatt tattatttta accgatattc ctaaggtata tatcaatttt cataaacctg
     9541 atgaaaaggc tctggatagg atgagcataa ccgaagcaag aagatacctt tctgataatc
     9601 aattttcgga aggaagtatg gcgccaaaag taagagcctg catcaagttt gtcgaaaaat
     9661 caggaaaaga agccttgatt actatggcca gtgaacttgg taaacctggt gccggaacaa
     9721 ttatttatgc tgaataaatt atcaaccatc aaaataaaaa ggagatatac gtatggcatt
     9781 caatttaaga aatcgcagtt ttttgaaact tctcgatttt acatctgaag aaatcaaata
     9841 tttgcttggt ctttcaaagg agctgaagaa ggcgaaatat accggcacag agcatcagcg
     9901 cttgaaaggg aagaatattg tgttgttgtt cgaaaaggat tctacacgaa cgcgttgtgc
     9961 atttgaagtg gccgcgctgg atcagggagc acatgttacc tatattgggc cttcgggctc
    10021 tcaaatgggg aaaaa
//
LOCUS       DAOORU010000088         9979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8396_length_9979_cov_6.378678, whole genome shotgun sequence.
ACCESSION   DAOORU010000088 DAOORU010000000
VERSION     DAOORU010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9979
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8396_length_9979_cov_6.378678"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /gene="ftcD"
                     /locus_tag="PLF20_06590"
     CDS             <1..1425
                     /gene="ftcD"
                     /locus_tag="PLF20_06590"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOX80704.1"
                     /translation="CPLVPVANISMEETVKYAHRLAERIGKELNIPVYCYENAALKPE
                     RRNLADVRAGEYEGLAEKLANPDWKPDFGPAEFNPWTGAIAIGARDFLIAVNFNLNTT
                     SIRRANAVAFDVREKGRPLREGNSIKGKIKKDKEGNEIWIPGLLKGTKAIGWYIDEYG
                     IAQVSMNITNISLTPLHIAFETVCEKARERGLRVSGTEIVGLVPARAILDAGRYFLHK
                     QQRSLGVSDKEIIKIAIKSMGLDDLKPFRPEEKIIEYMLESDVKEKKLIDMSCSDFTY
                     ETASESPAPGGGSISAYIGALGASLGTMVANLSAHKPGWDNRWEEFSHWAVKGQNIIR
                     DLLLLVDEDTRAFNQIMEAYGLPKTTEEEKSTRQKAIQQAMLYATQTPFNTMEKALEA
                     FEVIKTMAELGNPNSVSDAGVGAICARSAVIGAFLNVKINAAGLKNKKLAEDFIRRGN
                     EIERKAIEIETEILQMVNEKIGNI"
     gene            complement(1916..3232)
                     /locus_tag="PLF20_06595"
     CDS             complement(1916..3232)
                     /locus_tag="PLF20_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004314588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOX80705.1"
                     /translation="MKNTPIDYQIVRDCISKNGLISVGKASIREIKKLVDDIEQASGQ
                     RFIRMEMGNPGLPPAAPGVKAQIEALEKGIASEYPDIQGIPPLKKEISRFLKLFVNID
                     VDPQYCFATVGSMQGSFASFLTVNRMTDGKNKTLFIDPGFPVHKQQVKVLGQPFKSFD
                     VYDYRGERLRDKLEEELADGTVASLIYSNPNNPAWFCFNEQELQIIAEVATKHDVVVI
                     EDLAYFGMDFRKDYGQPGQPPYQPSIARFYDKYILLISSSKIFSYAGERIACLIISPK
                     LYHTNTPDLKKYYGTDSFGHAFVFGTLYTLSSGTAHSPQCALSHLLKLVNDGEYHFLN
                     EVREYGEKAAIMKKMFLENGFRIVYDKDLDVPIGDGFYFTFSYPGFSGDELLEEMIYY
                     GISAITLDITGSQRKEGIRACVSLVHRDQFPDLECRLKRFHIDHPI"
     gene            complement(4018..5709)
                     /locus_tag="PLF20_06600"
     CDS             complement(4018..5709)
                     /locus_tag="PLF20_06600"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOX80706.1"
                     /translation="MDSKDLLRKYFVQLYGELSITLLEKFLAELESTDGLSLDPLPSQ
                     WYKDAVIYSTYVDLFDKDFQGLTDRLNYLKQLGIDCLWLLPILDSPMRDAGFDIRDYR
                     CIRRSLFGLPEDAPAEVTQQLFHEFLNEAHQKNIRVIFDIALNHTSDQHSWFQESRKG
                     PDNAYRNYYIWNKDSNKYNETRLLFKGLCPSNWEYDNGWYYFHRFYEFQPDLNYRNPL
                     VLLEMSRILVFWLSQGIDGFRADAIPFLWKEDGTDCENLPQTHIIVKFFRAVIDIIRP
                     GALLLAEACQPPKEVVKYFGEGDECHAGYHFPLMPRIFISMALADYQPVRKVLNPKVT
                     PPIPEICQWINFLRLHDELTLEMVSPEERHIMNAYYRHDLRWNFREGEGISARLAELF
                     RFDPRKILQAFSIMLTLPGSPVIYYGDEVGKGNDEEWYEYMKSVSGHPDSRFYVRGPI
                     YWEEVEIALGNPSSFPAKIYTGLQQMLKIRRKSKVFGHRAIQWLDDIPCEILAFVRYY
                     QNEKILVVHQLANTTGEFLWPEFVVAPIDLFTNKPIDLHLPLALHPFDFFWIKYS"
     gene            complement(5829..7367)
                     /locus_tag="PLF20_06605"
     CDS             complement(5829..7367)
                     /locus_tag="PLF20_06605"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HOX80707.1"
                     /translation="MFRNYSFFPLCFFVFFSFIACNKEVKDKTSSSELVLYDSAIVFY
                     YPDGKFEDSLKNALGLEEFHHLRDSLTQFNFIWRKWADSLRLKQIVTDARLIEFKIGN
                     GIAYTIKLTKLCQPWGIILFYKNLYPEFITHPPTFPPAVHTSETQTLKQQSKKSAISR
                     TSIQNDDHLSSSKKPADSLPFLKRLSAHIIQVFTPPKEFTYEGPSASAAFHGKVLPQK
                     VVSAFVDNDVFLGTDLWYTHGANITWINPAWQSNPLTFLLHPVIGKSYDYYGLLIRQN
                     FYTPVYNTYEEIQYNDRPFASYFYLGQFKVSNFPSFRMKFSTEFDIGMLGPSSLGQEV
                     QTFVHNSNKKPIGWKHQIHDDIVLQYQAEIEKSLFFVPGHDITLESSLQAGTLYDKLS
                     AGFAYRFARFDRRYTNDLPQRNKSVIWKDFFAGAQIEFFTKVEATTVFYDATLQGGMF
                     NHSSPYTLREKDVERYLMMTSSGITTMFKGISLGANVYLLSPEFAQQKRWHKWGRIIL
                     SIAY"
     gene            complement(7501..9207)
                     /locus_tag="PLF20_06610"
     CDS             complement(7501..9207)
                     /locus_tag="PLF20_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/sugar symporter"
                     /protein_id="HOX80708.1"
                     /translation="MNNMQFGTIDYVIFILYILTIIGVGLWVSRTKKGAQKTSQDYFL
                     ASKALPWWAIGSSLIAANISAEQFIGMSGSGFAIGLAIASYEWMAAVSLLIVGKFFLP
                     VFIEKGLYTIPEFIEKRYSTQLKSILAVFWIALFIFVNLTSVLFLGSKALDTIIGTGN
                     GDLIMYSIIGLALFAAAYSLYGGLSAVAWTDVIQVILLIGGGLVTTFLALGHVSPDGG
                     IISGFKHIYEVAPEKFSMILQKGEIITPNGEDAYNYLPGLAVLIGGMWVANLYYWGFN
                     QYIIQRTLAAKSLKEAQHGIIFAAFLKILIPLIVVIPGIIAFVLNADPTGHITPQSAD
                     PTFFNAMGAVDNDRAYPWLVKTFIPTGLKGLVVAALVAAIVSSLASMLNSTSTIFTLD
                     IYKPYFSKNASEKELVTVGRITSAIALIIAIFIAPLLGNIKQAFQYIQEYTGIVSPGI
                     LAIFLLGLFYKKATNRSAIFGALFSIVFAFYLKIGQNGWMAGTPLAGFFPSFPWMDQM
                     LIVCLGTMLVMVIYSYFEARGVNDPKGIPLKKSTFHTDSVFNILATVVILILTAIYAI
                     FW"
     gene            9426..>9979
                     /locus_tag="PLF20_06615"
     CDS             9426..>9979
                     /locus_tag="PLF20_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HOX80709.1"
                     /translation="MKISENFFGRIEEKEIKIFELENPKGSKVSITNFGAIVQSLYVR
                     DREGNFEDIVLGFDSLEDYIQQTSYFGAIAGRYANRIAFAQFTLDGKTYQLSANDGNH
                     HLHGGFKGFDKVIWDARPYLDTTGACLELHYISKDGEEGYPGTLDVYVTYCFTLDDEL
                     KIYYQATTDHPTILNLTHHSYFNL"
BASE COUNT         3236 a         1969 c         2008 g         2766 t
ORIGIN      
        1 tgtccgttgg ttccagtggc aaatatttcg atggaagaga ctgtaaaata tgcccatcgt
       61 ttggcagaaa gaatagggaa ggaattaaac attcccgttt attgctatga aaatgcagca
      121 ttgaaacccg agcggcggaa tctggccgat gtaagggcag gagaatacga aggtctggcc
      181 gaaaaacttg ccaatcctga ttggaagcct gattttggac cggctgaatt taatccatgg
      241 acaggtgcca ttgccatagg agcccgcgat tttctcattg ccgtaaactt caatttgaat
      301 actacatcta ttcgcagggc taacgctgta gcattcgacg taagagaaaa aggacggcct
      361 ttgcgcgagg ggaattccat taaaggaaaa ataaaaaaag ataaagaagg caacgagatc
      421 tggatacctg gcctcttgaa aggcactaaa gctattggat ggtatatcga tgaatatggt
      481 atagcccagg tttcgatgaa tattaccaat atttcactca caccccttca tattgctttt
      541 gaaaccgtat gtgaaaaagc cagagaaaga gggctcagag tcagtggaac cgaaatcgta
      601 ggactagttc cggcgcgagc tatactcgac gcaggccgtt atttcctgca caaacagcag
      661 cgaagtctcg gcgtttcgga taaagaaatc atcaagattg cgattaaaag catgggtctc
      721 gatgatttga agcctttccg acccgaagaa aaaatcatcg aatacatgct tgagtccgat
      781 gtaaaagaga aaaaactcat cgatatgagt tgttctgatt ttacttacga aacggcttca
      841 gaaagtcccg ctcctggtgg tggatccata tctgcctata ttggtgccct tggagcatcg
      901 ttgggaacca tggtggccaa ccttagcgct cataagccag gctgggataa tcgctgggaa
      961 gaattcagcc attgggccgt aaaaggacaa aacatcattc gagacctcct tttattggtg
     1021 gatgaagata cacgagcatt caaccaaatt atggaagcct atggtttgcc taaaaccaca
     1081 gaagaagaaa aatctactcg acaaaaagct atacagcagg ccatgttata tgctacgcaa
     1141 acacccttca atacaatgga aaaagctctg gaagctttcg aagtgatcaa aacaatggca
     1201 gagttaggta atcccaattc ggtgagtgat gcaggagttg gtgccatatg tgcccgctcg
     1261 gccgttatcg gagctttctt gaatgtaaaa atcaatgctg caggattaaa gaacaaaaaa
     1321 ctggccgaag attttatccg tcgaggaaat gaaattgaac gaaaagccat tgaaatagaa
     1381 accgaaatcc ttcaaatggt gaatgaaaag attggaaaca tttaaaatct aattatttgt
     1441 agagtttttt gaatatctta tattctctat gtaagaggtg gaaaatttta tcaatacagt
     1501 tagaggtatt caaaatttat ctgaattatc ctaaaaatgc atgatccgaa gataatcgaa
     1561 catctttcgc tatcatattt cttatacggg taaagctata tcaagggcaa aatgaagagc
     1621 ccgagtccga gaaactgaac gatgcggcaa tgattcgact tttttctatt caaaactccg
     1681 taatcttctt tgcggacatt gtaaggtata gaaaaccaac cacaaccttc gtcaaaataa
     1741 tatcaggcag aaaaaagcaa taacaccttg catgatctgt aacccatgct ccataatact
     1801 tttaactttt ccatccactc gtcctcagcc ctaaaaatac cattcccctt ggaacatgcc
     1861 actcatgaaa cgtgaattaa aattttaaga cgaggaaaac gtagccttct tttttctata
     1921 taggatgatc tatatgaaac cttttcaggc ggcattcgag atcgggaaat tgatcacgat
     1981 gaaccaaaga aacgcaggct ctgattcctt ctttgcgctg acttccggta atatccaagg
     2041 taatggcgct aatcccataa tagatcattt cttcgagcag ctcatcccca ctgaatccgg
     2101 gataactaaa agtaaaataa aaaccatcgc caattgggac atcgaggtct ttatcataaa
     2161 caatacggaa gccattttcg aggaacattt tcttcattat tgcagctttc tcaccatatt
     2221 ctcttacttc gttcagaaaa tggtattccc catcattcac taattttaac agatgggata
     2281 gtgcacactg gggtgaatgt gctgttccgc tgctaagagt atataatgta ccaaaaacaa
     2341 aggcatggcc aaaactgtcg gtaccgtaat acttttttaa atctggagta tttgtatgat
     2401 ataactttgg tgaaataatg agacatgcga ttctttctcc tgcataacta aaaatcttgg
     2461 aactcgatat caagagaatg tatttatcgt agaaacgagc tatgcttggt tgatatggag
     2521 gttggccggg ctgaccataa tcctttctaa aatccatccc aaaataggct aaatcttcaa
     2581 tgaccaccac atcgtgtttg gttgctacct cggcaatgat ttgtagttct tgctcgttga
     2641 agcagaacca tgcaggatta ttgggattgg aataaataag ggaagccacc gtgccatcgg
     2701 ccaattcctc ttccagttta tctcgtagtc tttcacctct ataatcataa acatcgaacg
     2761 atttgaaagg ctgtcccaaa actttaacct gttgtttatg aactggaaac cccggatcaa
     2821 tgaataaggt cttgttttta ccgtcggtca tgcggttaac ggtgagaaaa gatgcaaaac
     2881 taccctgcat gctgccaaca gtcgcaaaac aatattgtgg gtcgacatca atatttacga
     2941 aaagctttaa gaatcgggaa atttcctttt taagcggagg aataccttgg atatcagggt
     3001 attcggaggc aatacccttt tcaagagctt ctatctgggc ttttactcca ggcgctgcag
     3061 gaggtaaacc cggattaccc atttccatac ggataaacct ttgaccactg gcctgttcaa
     3121 tatcatcaac cagtttcttg atttcacgaa tggatgcctt tcctaccgaa atcaatccat
     3181 ttttcgagat acaatctcta acaatttgat aatcaattgg agtattcttc ataatataaa
     3241 tagttttgca aggctaaaat atgcaataaa ttcataactg acaatcacaa aaacaattca
     3301 tcccatttct ctaagggaac tttccatagc agaagcgcag aaatacgcta caagcgactt
     3361 aaattctcaa ggttcgaggc tgaagatagc catagaagga ctaagttttg aaaaactcgg
     3421 ggatgacaaa atgggaacca tcattttcga gccaaggtca gctttatttt tttatggatt
     3481 gaatttttga acatgtttaa tgtttccaac atcgatgaat ttgaatttcg aattgatctc
     3541 gaaaggtaaa tcaggtggca ggataaatcg aaagcgagcc cgcttgccgg gttctgagtt
     3601 ggaagtaatt gtttcctaaa agccttttcg ataatggcgg aaattaccga taataattcc
     3661 aaaagtaaat aagcatgatg aatgtgaaat gcattggtat gtcaggaagt ataaaacaga
     3721 aaatctattc cgcgattaga tacgatgagg agtaaggtca aatttgaatg aattccttct
     3781 tcgatcaata ttcgtgaact ctggggatag ggagattcga cgagttataa attcacatca
     3841 cgaacgattt gaattttacc ttcttttaaa tacaggttgc aagctcgaaa gcgccaaatc
     3901 tgccgagaaa gaattatcaa caataagaac aggttcttat ttctttcggt caaagtcaat
     3961 tcccgctcgt aaaatattga attatagtag atatattttt catgctatga ttctatttta
     4021 tgaatatttt atccagaaaa aatcaaatgg gtgtaaggca agggggagat gaagatcgat
     4081 aggtttattg gtgaaaaggt ctataggagc cacgacgaat tcaggccata agaattcgcc
     4141 cgttgtattg gcaagctggt gaaccaccaa aatcttctca ttttgatagt agcggacaaa
     4201 agccaagatt tcgcagggta tatcatcgag ccattggata gctcgatggc caaagacctt
     4261 tgattttctg cggattttca acatttgttg taaacccgta taaattttcg cagggaaaga
     4321 agaaggattt ccgagtgcaa tttccacttc ctcccaatag ataggccctc tgacgtagaa
     4381 gcggctatcg gggtgtcctg aaacagattt catgtattca taccattctt catcattacc
     4441 tttgcccact tcgtcgccat agtagattac cggtgagcct gggagagtaa gcataataga
     4501 aaacgcctgt aaaatttttc tggggtcgaa gcgaaaaagt tcggccagtc gtgctgatat
     4561 accctctcct tcacggaaat tccagcgtaa atcatgacga taataggcgt tcatgatatg
     4621 gcgttcttct ggagaaacca tctcgagtgt aagctcgtcg tgaaggcgga gaaaattaat
     4681 ccactgacag atttcgggaa ttggaggagt aactttggga ttcaacactt ttcggacagg
     4741 ttgatagtcg gccagagcca tcgaaatgaa aatccgtggc atcaatggaa aatgataacc
     4801 tgcatgacat tcgtcgccct ctccaaaata tttcacgacc tccttaggtg gttgacaggc
     4861 ttcggccagc aaaagtgccc ctggccgaat tatatcgata actgctctga aaaatttcac
     4921 gatgatgtgt gtctggggca aattttcgca gtcagttcca tcttccttcc ataaaaaagg
     4981 aatggcatcg gcacgaaaac cgtcgatccc ctgagagagc cagaaaacca atatgcggct
     5041 catttccaat aaaaccaaag gattgcgata attcaaatcg ggttgaaatt cataaaaacg
     5101 atgaaaataa taccaaccat tgtcgtattc ccagttactt gggcaaagac ccttgaacag
     5161 aagacgggtt tcattgtact tgttgctgtc cttattccag atatagtaat ttctgtaagc
     5221 attatcgggc cctttgcgag actcctgaaa ccaggaatgc tggtcggagg tatgattcag
     5281 agctatatcg aaaatcacac gaatattttt ctggtgagcc tcattcaaga attcgtgaaa
     5341 caactgctgg gtaacttcgg caggagcatc ttcgggcaaa ccaaacaaac ttcgacgtat
     5401 acagcggtaa tcccgaatat cgaagccagc atctctcatg ggagaatcaa gaataggtaa
     5461 aagccataaa caatcaatac ccagttgttt cagataattc aatcgatcgg ttaaaccttg
     5521 aaaatcctta tcgaacaaat ccacataggt cgaataaatc actgcatctt tataccattg
     5581 agaaggcaaa ggatcgagag ataacccgtc agtagattcc agttctgcga ggaatttttc
     5641 gagcagagta atagataatt ctccatagag ttgaacaaaa tatttccgaa gaagatcctt
     5701 cgaatccata ataaaagatg attaaggcta atgatttgcg aaaatacaca atggaataaa
     5761 aatatctaat tattccattc gaggatgata tttgaaaaaa tacacgactt ggtaaaagaa
     5821 ttcagtattc aataggcaat gctcaaaata attctacccc atttgtgcca gcgtttttgt
     5881 tgggcaaatt cggggctaag cagataaacg ttagctccca gactaatgcc tttgaacatc
     5941 gtagtaatgc ctgagctggt catcatcagg tatctttcca catctttttc tctaagggta
     6001 taagggctcg aatgattgaa cattcctccc tgtaaagtgg catcataaaa aacagtagta
     6061 gcttcaacct tagtaaaaaa ttctatttga gctccagcga aaaaatcctt ccatattacc
     6121 gatttattcc tttgaggaag atcattggta tagcgccgat cgaaacgggc aaagcgataa
     6181 gcaaatccgg cggatagttt atcgtataaa gttccagctt gcaaactgct ttctaaagtg
     6241 atatcgtgcc ccggcacgaa aaacaatgat ttttcaattt ctgcctgata ttgcaaaaca
     6301 atatcatcat gtatttgatg cttccatcca atgggtttct tattcgaatt gtgcacaaaa
     6361 gtttgaacct cctgtcccaa agaacttggg cctaacatac cgatatcgaa ctcggtggaa
     6421 aatttcattc gaaaggaagg aaagttcgaa accttgaact gacctaaata gaaataagaa
     6481 gcaaaagggc ggtcattata ctgtatctct tcataggtat tataaacggg agtgtaaaaa
     6541 ttttgccgga tcagcaatcc ataataatca tatgattttc cgattaccgg atgaagcaag
     6601 aatgttaaag gattactttg ccaagcagga tttatccagg tgatattagc cccatgggtg
     6661 taccataggt cagttccgag aaatacatcg ttatctacaa aggcactcac tactttttga
     6721 gggagaactt taccatgaaa agcagcagaa gccgaaggtc cttcataagt aaattcttta
     6781 ggtggtgtaa aaacttgaat aatatgagct gataaacgtt ttagaaatgg gagcgaatcg
     6841 gctggttttt tcgatgagct taaatgatcg tcgttttgaa tgctagtgcg acttatggca
     6901 cttttcttcg attgttgctt caatgtttgt gtttcagaag tatgaactgc tggaggaaaa
     6961 gtgggtggat gagttatgaa ctcgggataa agattcttat aaaataaaat aataccccaa
     7021 ggttgacata atttagtaag ctttatggtg taagcaattc cattccctat tttaaattca
     7081 atgagtcgag catcggttac gatttgtttt aaacgcaggc tatcggccca tttgcgccat
     7141 ataaaattaa attgtgtaag gctatctctc aaatgatgga attcttcgag tccaagcgca
     7201 ttcttcaaac tatcttcgaa cttaccatct ggataataaa agacgatagc agaatcgtaa
     7261 agcactaatt cagaagaaga tgttttatct tttacttctt tgttgcatgc aataaagctg
     7321 aagaaaacga aaaagcagag agggaaaaaa gagtagttac gaaacatcat tgatgattca
     7381 actcgcatga atgattaaac ggatggcaaa ggtaagaggt tattcataaa ttgagggaga
     7441 ttttaatatg gcgaaggaag ctcgatcctt cgccatatta aaacattctt tttacaaacc
     7501 ttaccagaat atggcataaa tggccgtcaa aataagaatt accacagtag ctaaaatgtt
     7561 gaataccgaa tcggtatgga aagtgctctt tttcaaagga attccctttg gatcattcac
     7621 acctcgtgcc tcaaaataac tataaataac catcaccagc atggtgccca aacatacgat
     7681 cagcatttga tccatccatg ggaaggatgg gaaaaatcct gcaagtggtg tacccgccat
     7741 ccatccgttc tgtcctattt ttaaatagaa agcaaacacg atactgaaga gagctccaaa
     7801 gattgcagaa cgattggtag ccttcttata gaataatcct aaaaggaaaa tggccagaat
     7861 tcccggactc acaataccgg tatattcctg tatatattga aatgcttgct taatatttcc
     7921 cagtaagggt gctataaata ttgcaatgat aagagcaatc gcagaagtaa ttcgacctac
     7981 agtcaccaat tccttttcgg aagcattctt cgagaaatag ggtttataga tatcgagtgt
     8041 aaaaattgta ctggtagaat tcagcataga ggcgagggaa gaaactatgg cggccaccaa
     8101 cgcagcaacc acaagaccct tcagtcctgt tggaataaat gtcttaacca accagggata
     8161 cgcccggtca ttatcaacag ctcccattgc attaaaaaag gtaggatcgg cactttgggg
     8221 ggtaatatgt ccagtgggat cagcattcag tacaaaggca ataattcccg gaataactac
     8281 aatcaaagga ataagaattt tgagaaaagc ggcgaaaatt attccgtgct gtgcttcttt
     8341 tagcgatttg gccgctaagg ttctttgaat gatatactga ttgaatcccc agtaatacaa
     8401 attggcaacc cacatgcctc caatcagaac agccaagccg ggcaagtaat tataagcatc
     8461 ttcgccattc ggagtaataa tttctccctt ttgaagaatc atgctgaatt tttccggcgc
     8521 aacttcataa atatgtttga atccagagat aattcctcca tcaggagata catggcccag
     8581 tgcaagaaag gtagttacga gtcctcctcc tatgagcagt attacctgaa tgacgtcagt
     8641 ccatgctact gctgaaaggc caccatatag ggaataggct gcagcaaata atgctaatcc
     8701 aataatagag tacatgatta aatcgccgtt tcccgttcca atgatagtat cgagagcttt
     8761 actgccaaga aataaaacgg aggtgaggtt cacgaaaata aacagcgcaa tccagaacac
     8821 cgcaagaatg gatttcaact gcgtgctgta acgtttttcg atgaactcag gaatggtgta
     8881 taaacccttt tcgataaaaa ctggcaggaa aaatttaccc acaataagca acgacacagc
     8941 cgccatccat tcataagaag caatagccaa gcctattgca aatcccgaac ccgacatccc
     9001 tataaactgt tcggcggaaa tattggctgc tatcagtgaa gaacctattg cccaccaggg
     9061 caaagccttg ctcgccagaa aataatcctg agaggttttc tgcgctccct tcttagtgcg
     9121 tgacacccat aatcccacac caatgatggt gagaatatat aaaataaata ttacataatc
     9181 aatcgtacca aattgcatgt tgttcatagt cggtcatatt tattttttta tcaattttgg
     9241 gcgcaagata taaaaatatt ttttccgtga agcaaatcct ctcttttctg attgtaaaaa
     9301 atacaataat tcaatagttc accatgaaaa caagctctaa aaaatttgtt tatctaactg
     9361 tatttgttat tattacaagt cagcttactc tttcctcatg ctttaatatg aaatctgaca
     9421 ctactatgaa aatctccgaa aatttttttg gtcgaataga agaaaaagaa attaaaatct
     9481 ttgaactcga aaatccaaag ggaagcaagg taagtataac gaattttggt gctattgtcc
     9541 aatcgttgta tgtaagagac agggaaggaa atttcgaaga tatcgtcctt ggattcgaca
     9601 gtttggaaga ttatatacag caaacctcat attttggagc cattgccggc cgctatgcca
     9661 atcgtattgc ttttgcacaa tttaccctcg atgggaaaac ctatcaatta agcgccaatg
     9721 atgggaatca tcatcttcat ggaggtttta aaggattcga caaggtcatc tgggatgcaa
     9781 gaccatattt agataccaca ggcgcatgtc tcgaattgca ttacatcagt aaggatggtg
     9841 aagaaggtta tcctggcaca ctggacgtat acgtaactta ttgttttaca ttagatgatg
     9901 agctcaaaat ttattaccag gccaccaccg atcatcctac catacttaat cttacccatc
     9961 atagttattt caatctcag
//
LOCUS       DAOORU010000089         9958 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8413_length_9958_cov_6.157831, whole genome shotgun sequence.
ACCESSION   DAOORU010000089 DAOORU010000000
VERSION     DAOORU010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8413_length_9958_cov_6.157831"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="PLF20_06620"
     CDS             <1..783
                     /locus_tag="PLF20_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80710.1"
                     /translation="TTDYARILGDPGYAHEIERKNNIVNEFYSNTYQAYRSGQYYLVV
                     MNCDEAASRFLNHPLLPKFEFLKSLAIGKIDVIDSLAVNLQRFIMHYPKHELAAHARN
                     ILDKLSKLDPSITSRIQLSGDSISLGTSAEPPKTPYLVETQSDHFVLLMVNPLQTDIN
                     ALKIRIADFNERAFRLENFYTSDILLNDTLQIISIGVFKNASKAMDYYHHILDNEYVF
                     SMLKEKFHETFVISTKNYQTFYQDRDISKYKSFFNKEYLKKN"
     gene            835..1215
                     /locus_tag="PLF20_06625"
     CDS             835..1215
                     /locus_tag="PLF20_06625"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOX80711.1"
                     /translation="MAKNTIIESPIVNIIGSMTSIEGDVSCGGDIRIEGQLKGKLECK
                     GKVVIGESGVVEGEIYCRNGDFAGKIKGSVNTSELLTLKSTVVLQGDITCSKLAIEPG
                     ARFSGKCTMELPENVQAAGQNEKE"
     gene            1202..1456
                     /locus_tag="PLF20_06630"
     CDS             1202..1456
                     /locus_tag="PLF20_06630"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HOX80712.1"
                     /translation="MKKNDIKPSLFNYAKYSSLAFQMLVVILIGVLGGIKLDQYLQLK
                     FPVFTILFSIAAVFIATYLGMKDFIKRPPKPSQKIDHNDE"
     gene            1446..1835
                     /locus_tag="PLF20_06635"
     CDS             1446..1835
                     /locus_tag="PLF20_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80713.1"
                     /translation="MTNEFKHYLRKLIILSVFLAILGTILKFVLPKGIITPAMPFILI
                     FFMAHTLVYNNMAVKMIHQNPKKFVNFYMLSTVGRLILFVLLLVLYAFLVPKDFKAFT
                     VCFFIFYLIYTFFELSTLLPQLRKKKP"
     gene            1847..2260
                     /locus_tag="PLF20_06640"
     CDS             1847..2260
                     /locus_tag="PLF20_06640"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HOX80714.1"
                     /translation="MIYLTRRETFNAAHRLFRPEYSDKKNLEIFGKCSNPNWHGHNYT
                     LYITVKGIPNSETGMAINLKELSQIIQKYIIEKVDHKNLNTEVDFMYGRMTSIEMIAI
                     AFWEQLKQPILAIGAQLYKIRLEETENNFVEYFGQ"
     gene            2352..2969
                     /gene="folE"
                     /locus_tag="PLF20_06645"
     CDS             2352..2969
                     /gene="folE"
                     /locus_tag="PLF20_06645"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HOX80715.1"
                     /translation="MIDENSLGGYEKIDKYNYETLEKLSFHYYEILKLIGEDADREGL
                     LDTPMRVAKAMQFLTHGYDLEPKEILLSARFKEDYRQMVIVKDIEIYSLCEHHMIPFI
                     GKAHVAYIPNGYITGLSKIARVVDAYARRLQVQERLTTQIKECIQEALNPLGVAVVIE
                     AKHLCMAMRGVQKQNSVTTTSDFTGAFTRKETREEFIHLIGSELS"
     gene            complement(3228..3449)
                     /locus_tag="PLF20_06650"
     CDS             complement(3228..3449)
                     /locus_tag="PLF20_06650"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HOX80716.1"
                     /translation="MKAGKWFIIIGLVLIIIGLMLYFLPSLFRWFGHLPGDIRIEKGN
                     TRIYFPLTSMIVISLVITVIINLFRFFKS"
     gene            complement(3579..4202)
                     /locus_tag="PLF20_06655"
     CDS             complement(3579..4202)
                     /locus_tag="PLF20_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HOX80717.1"
                     /translation="MNEDLLDKGIELPIMEEFYTLQGEGIQTGRAAYFVRLGGCDVAC
                     EWCDTKESWNSNLFPPKKVDDMILRVMVTKAQSVVVTGGEPLNWNLDYLCQKLKENNI
                     LTFLETSGSQPLSGVWNWICVSPKKITSPLQHILDKANELKVIIQNSTDFAWAEEIAS
                     KVKTDCYLLLQPEWSRQQQITPAIIDYIKKFPQWRLSLQVHKFLKIP"
     gene            complement(4212..5396)
                     /locus_tag="PLF20_06660"
     CDS             complement(4212..5396)
                     /locus_tag="PLF20_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80718.1"
                     /translation="MRSYFRLYGLSVVLFLLVSFELLGQNNDLSFPIRTQYYNPPIHI
                     RKLPAALSETSGLIYYNGLFWTHNDSGGEPKIYGIDTLKGKIIQTIHLNASHKDWEDI
                     AQDDDYIFIGDFGNNLGTRNDLCIYRIKKEQIPQQGDVSLTPEVIRFRWDKSLETSLP
                     DRKTNFDCEAMFCLHDRLILLTKNWGNQRTSIYSLPKIPGDYSPVFLLDFNANGLITG
                     ADFLEDDQTLALCGYKSYVPFIWLIKDFQTIDTRGWTALRLELDDRAGSQTEGICFGP
                     AKSLYVSSEATAFYPALIFKLDYPALSSRLPLRGEDVFSYQYVFDNEQLRIITSCKKI
                     KNYKVQILDNKGNILKEKKMKCTGNMDVNVWPLHESNKKMILRMKGGKYEVTSILDSD
                     FL"
     gene            5581..6840
                     /locus_tag="PLF20_06665"
     CDS             5581..6840
                     /locus_tag="PLF20_06665"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOX80719.1"
                     /translation="MLKKIAGTTLVRFISALASLIILSMNARVLGPPGLGIISLIVVG
                     IAIIQNISNIIGGSALVYLASRYDNFYLYLLSSLWALISASIGTVIMSLTKTIPAEHV
                     YNVLFLSLLASLINIQQMLLLGHERVKAYNWSSLLQSLVNALALFYFFFIANDRTVDG
                     FVHSLYIAFGVCFLLTYFMIFPSLKITAIEGLVPLVKEIVRLGGSIQIAGLLQTLNYR
                     FSYFLLKKFFGTGTLGRFDAGVKLSEGIWLAAKSIALVEYSRISNESDEQKSYRMAIA
                     LFKLSFFITLAGIILLLLLPTVAFEFLLGQGFPDIKWIILSLSPGILAIASGMIFSHL
                     FSGTGKPHYNIIGSGIGLITLIAAGYLFIPKFGLMAAGLVTSFSYLSSLACQIYLFRK
                     MSGYAWSVFIPRRSDFAFLFTKTNRKP"
     gene            7144..7698
                     /gene="rfbC"
                     /locus_tag="PLF20_06670"
     CDS             7144..7698
                     /gene="rfbC"
                     /locus_tag="PLF20_06670"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008658903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOX80720.1"
                     /translation="MKIIETRIPGLLIVKPEVFEDERGYFFESYNQQKFQELGIDVVF
                     VQDNESKSQKNVIRGLHFQKPPFAQGKLVRVMKGSVLDVAVDLRKGSPTYGQWDSIVL
                     SESNKFMYWIPEGMAHGFRTLQDDTLFFYKCTHFYNKGSEQALRWNDPALNIDWQLME
                     PPILSEKDRLSPFFKDFVSPFEYE"
     gene            complement(7950..8023)
                     /locus_tag="PLF20_06675"
     tRNA            complement(7950..8023)
                     /locus_tag="PLF20_06675"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7987..7989),aa:Met,seq:cat)
     gene            complement(8096..9004)
                     /locus_tag="PLF20_06680"
     CDS             complement(8096..9004)
                     /locus_tag="PLF20_06680"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOX80721.1"
                     /translation="MRQDGKFSAFVYLLVILAMVFWGMSYVWTTMVFRYLNPITTIFL
                     RLVISSALLFFIFGLTHQLQPVRKKDYKLFLFAALFEPFLYFLGEGYGVKYSTPTISA
                     IIISTIPIFSAIAASIFLKEVLQPLQIFGFFLSLGGIIFMLLGPNFSFQFNPIGIVSL
                     FFAVATAVIYAVLLKRLTMLYSSFTIVAWLNFIGAFYFLPLVLIVEPSQLTLLHFEWG
                     LWYPLIMLAVFASSLAFIFFTFGMHHLGVSRTSIFTNFIPVFTAIFSYFKIHEKLTVN
                     ILVGMLMVIVGVIITQKNVRIKTLRA"
     gene            9059..9445
                     /locus_tag="PLF20_06685"
     CDS             9059..9445
                     /locus_tag="PLF20_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80722.1"
                     /translation="MKKFKIKGKKYSSHDFDYGDEETEGKENRDWGLEIGDWRLEIGG
                     WRLEIGDWGLEIGDWRLEIGIWRLEVGDWRLEIGDWRLEIGDWGLEIGDWNLEIGDWG
                     LEVGICLQFGISKLSVVSCNLRKSAD"
     gene            9478..9672
                     /locus_tag="PLF20_06690"
     CDS             9478..9672
                     /locus_tag="PLF20_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80723.1"
                     /translation="MLTKGRENRGREEGRLEIGGWRLGIGDWRLEVGDWRLEIGDWGL
                     EIEGWRLEVGDWRLEVGICL"
BASE COUNT         3081 a         1728 c         2072 g         3077 t
ORIGIN      
        1 actaccgatt atgccagaat tttaggcgac ccgggttatg ctcatgagat tgaaaggaaa
       61 aataatatcg tcaacgaatt ttattcgaat acttatcaag cttatcgatc gggccaatat
      121 tatctggtag taatgaattg cgatgaggca gccagcaggt ttctcaatca tcctcttttg
      181 ccgaaattcg aatttttaaa gtcattagct attggaaaaa ttgacgtaat cgattcgttg
      241 gcggtcaatc tgcaacgttt tatcatgcat tatcctaaac acgaacttgc tgctcatgct
      301 cgcaacattc tcgataaact ctccaaattg gacccttcga ttacctcaag aattcaacta
      361 tccggtgata gtatatctct gggtacatct gccgaacctc caaaaacgcc ttatctggtc
      421 gaaacacaaa gcgatcattt tgtactgctt atggttaatc ctctacaaac cgatatcaat
      481 gcattgaaaa tcaggattgc cgattttaac gaacgagcct ttcgcctcga aaatttttat
      541 acttcagata ttttgcttaa cgacactttg cagatcatca gcattggtgt ctttaagaat
      601 gcttcgaagg ccatggatta ttatcaccac attctggata atgagtatgt tttctcgatg
      661 ttgaaagaga aattccacga aacattcgtt atttccacga aaaactatca aaccttttat
      721 caggatagag atatttcaaa atataagtct ttctttaata aggaatatct gaagaaaaat
      781 taaggatttc gataaaatca gtgattttta gtgtaaattt gaaaaaaata tactatggct
      841 aaaaatacta ttatagagag ccccattgtg aatatcatcg gttcgatgac atctatcgaa
      901 ggcgatgtga gctgtggagg cgatattcgt atcgaaggtc aattaaaggg caaactcgaa
      961 tgcaaaggca aagtcgtgat tggggaatcg ggagtcgtag aaggggagat atattgccgc
     1021 aatggtgatt ttgccgggaa aatcaaagga agcgtcaata cttccgaact cttaacctta
     1081 aaatcaaccg ttgtgcttca aggagatatt acttgtagta aattggctat agaacctggt
     1141 gcgcgtttta gtgggaaatg caccatggag cttcccgaaa acgttcaagc cgctggccaa
     1201 aatgaaaaag aatgatatta aaccctcact gttcaactac gccaaatact cttcacttgc
     1261 ttttcaaatg cttgtggtca tccttattgg tgttttgggt ggaataaaac tcgatcaata
     1321 tctgcaattg aagttcccgg tctttactat cctgttttcg attgcagcag tgttcatagc
     1381 tacttattta ggcatgaagg acttcataaa acgacctccc aaaccttctc aaaaaattga
     1441 tcataatgac gaatgagttc aaacattatt tgcgcaaatt aataatcctt tctgtatttc
     1501 tggctatact tggtactata ttgaagtttg tacttcccaa aggcattatt acaccggcca
     1561 tgccttttat ccttatcttt tttatggctc atacgctggt ttataataat atggcagtga
     1621 aaatgattca tcaaaaccct aaaaaatttg tcaattttta tatgctttct actgtcggcc
     1681 ggttgatttt attcgtgttg ctacttgtcc tctatgcctt tcttgtccca aaggatttca
     1741 aggccttcac cgtatgcttc ttcatttttt acttgattta cacttttttt gaactttcta
     1801 cccttttgcc tcagcttcgc aagaaaaaac cataagtatt gtcgcgatga tctaccttac
     1861 tcgccgtgaa actttcaatg cagctcacag gcttttcagg cccgaatata gcgataaaaa
     1921 aaacctcgaa atttttggaa agtgcagcaa tccaaactgg cacgggcata attatacctt
     1981 gtatattact gtgaaaggaa tacccaattc cgaaactggc atggccatca acttgaaaga
     2041 attaagccag attattcaaa aatatatcat cgaaaaagtg gatcataaaa acctcaatac
     2101 ggaagtagat ttcatgtatg gcaggatgac ttcgattgaa atgatcgcta ttgctttttg
     2161 ggaacaacta aagcaaccta ttttagccat aggagctcaa ctttacaaaa ttcgacttga
     2221 agaaaccgaa aataactttg ttgaatattt tggacagtaa atcaaacatt cctgatcaag
     2281 aaatgttaca tcaccaataa cccttagcat ttaccaacaa tatgaacaag tccgacaaga
     2341 ttattcacga tatgatagat gaaaattcgt tgggtggata tgaaaaaatc gacaaatata
     2401 attatgaaac actcgagaag ctatcatttc attattatga gattttaaaa ctgataggag
     2461 aagatgccga tcgtgagggt ttgctcgata cccccatgcg ggtagccaaa gccatgcagt
     2521 ttcttacaca cggctatgat ctcgagccca aggaaatttt gctctcggct cgttttaagg
     2581 aagattatag gcaaatggtg atcgtaaaag acatcgaaat ttacagcctg tgcgagcacc
     2641 acatgattcc gtttatcggg aaagctcatg tggcttatat acctaatgga tacatcacgg
     2701 gtttgagcaa gattgcccga gtggtcgatg cttatgctcg tcgcctgcag gttcaggaaa
     2761 ggctaactac ccaaatcaag gaatgcatac aagaagcttt gaatccccta ggtgtagctg
     2821 tagttatcga agctaaacac ttatgcatgg ccatgcgtgg tgtacaaaaa cagaattccg
     2881 taaccaccac ttccgatttt acaggtgctt ttacacggaa agaaacacgt gaagaattta
     2941 tccacctcat tggttctgag ttatcatgag ttttgaacgt aatttgttga gggatacccc
     3001 ttacctgtga taaagtgcga ggtgagaggg atttttgata aatggcgaaa accatagagg
     3061 tgggtgattg gaaagcatga attgttgata agttggaatt agtaagatgc agggtcataa
     3121 attcgagttg caacgctggc gtagtgaatc taaatagaat tcgaattctt agaatgttat
     3181 ttttgtgttt aatagggcga tatatgatag cttctttgca ctctttttca agatttgaaa
     3241 aaccgaaata aattaatgat gacggtaata acaaggctta tgacaatcat tgaagtaaga
     3301 gggaaataaa ttcgagtatt tcccttttcg atgcgaatat cgcctggaag atgtccaaac
     3361 catcgaaata atgacggtaa gaagtataac atcaatccga tgataatcaa cacaagtcct
     3421 ataataatga accatttgcc tgctttcata ttttcaacca attcgctgcg atgataatta
     3481 ttttaattta cgtgataaaa aaatgaaata atatttttta atgagttttt tattgaaaca
     3541 ttgcatcaga tttttgaact tgtctaacct tgatgctatt atggaatttt gaggaattta
     3601 tggacttgaa ggctgagtcg ccattgcgga aatttcttga tataatcgat aatggcagga
     3661 gttatttgtt gttgtcggct ccattcgggt tgaagcagca aataacaatc ggttttgact
     3721 ttcgaagcga tttcttcagc ccaagcaaag tctgttgagt tttgaataat gactttcaat
     3781 tcattggctt tatcaaggat atgttggagg ggggaagtaa tttttttagg tgaaacacag
     3841 atccagttcc atacgcctga aagtggctgc gaaccagagg tttcgagaaa agttagaata
     3901 ttattttctt ttaatttctg gcagagataa tcgagattcc agttcaaggg ttctcctccc
     3961 gtaaccacaa ccgattgagc ctttgtaacc atcacacgta atatcatatc atccactttt
     4021 ttaggtggaa aaaggttgga attccagctt tcctttgtgt cgcaccattc gcaggctaca
     4081 tcgcagccac ccaatcgaac aaaataagct gcacggccgg tttgaattcc ttcgccctgt
     4141 aaggtgtaaa actcttccat gatgggaagt tctatgcctt tatcgagtaa atcttcattc
     4201 ataaaattct atcataaaaa atcagaatca agaatagacg taacttcata tttgcctccc
     4261 ttcatcctaa gaatcatttt tttgttcgat tcatgaagag gccagacgtt tacatccata
     4321 tttcccgtac atttcatctt cttttctttt aaaatgttcc ctttattatc taaaatctga
     4381 actttataat ttttaatttt tttacaggaa gttatgattc tgagttgttc gttatcgaaa
     4441 acatactgat aggagaaaac atcttcgccc ctgagaggca gacgagaaga aagagcagga
     4501 taatcgagtt tgaatattaa cgcaggataa aatgccgtag cttcgctcga gacataaaga
     4561 gatttagcgg gtccaaaaca aatgccttcg gtttgggagc ctgctcggtc gtcgagttcc
     4621 agccgaaggg cagtccatcc tctggtgtcg atggtttgaa aatccttgat gagccaaata
     4681 aaaggcacat aacttttata accacataag gccagggttt gatcgtcttc gaggaaatct
     4741 gctccagtaa ttaacccatt ggcattgaaa tcgagtaaaa ataccggtga ataatcgcca
     4801 ggaatttttg gcaaagaata aatcgaagtt cgctgattac cccagttttt cgtaagaagg
     4861 attaagcgat cgtgcaaaca aaacatggct tcacaatcga aatttgtttt acggtctggc
     4921 aaactcgtct ccaaagattt atcccatcta aaacgaatca cttcaggtgt aaggcttaca
     4981 tcaccttgtt ggggtatttg ttcttttttg attcgataaa tgcataagtc gtttcgggtg
     5041 ccaagattat tcccaaaatc accaataaaa atatagtcat catcttgagc aatatcttcc
     5101 caatccttgt gagaagcatt caaatgaatg gtttggatga tcttcccttt taaagtatct
     5161 attccataga ttttgggctc cccgccgcta tcgttatgtg tccagaataa accattatag
     5221 tagatgaggc ctgaagtttc actcaaggcg gctggaagtt tgcgaatatg tatgggtgga
     5281 ttgtaatatt gtgttctgat agggaaactc aaatcattgt tttgccccaa tagttcaaat
     5341 gaaactaaca ggaacaatac cactgataaa ccatataatc tgaaatacga tcgcatttct
     5401 gtaattttag gtattttaga atttataaaa ttagacataa tattaattga acatattttg
     5461 agacgctcag atttttttgg caaatttaca ctttaagcaa tagagctttc ttcggtgggc
     5521 ggactcatcg tgaggatccg attatctgat atatatttgc cccataaagc ttatgggagg
     5581 atgctaaaaa agattgcggg gacaacctta gtgaggttca tcagtgcttt ggccagccta
     5641 atcattctga gcatgaatgc gagggtatta gggcctccgg gcttaggaat catctctttg
     5701 attgtagtgg ggattgctat tattcagaat atcagtaata ttattggagg ttcggccttg
     5761 gtttatctgg cttcgcgata tgataatttt tatctttact tgttgtcgtc gttgtgggca
     5821 cttatttctg cttctatagg aacggtaata atgtcattga ccaaaaccat tccagccgag
     5881 catgtttaca atgtactttt tttatcacta ttggcatcgc tcatcaatat tcaacaaatg
     5941 ttgctcttgg ggcacgaaag agtgaaagcc tacaattgga gcagtttgtt gcaatcatta
     6001 gtcaatgctc tggcactgtt ttatttcttt tttattgcaa atgatcgaac agtggatggc
     6061 ttcgtccact cgttatatat agcgtttggc gtctgctttt tacttaccta tttcatgatt
     6121 tttccttccc tgaaaattac cgctattgag ggattggtcc ctttggtgaa ggaaattgtg
     6181 cgtttgggtg gctctataca gatagcaggt ttattgcaaa ccttgaatta tcgttttagt
     6241 tattttttat tgaaaaaatt ttttggtaca ggtactttgg gacgctttga tgcaggtgta
     6301 aaactctcgg agggaatctg gctcgctgct aaaagcatag cactggttga atactccaga
     6361 atttcaaatg aatccgatga acaaaagtcg tatcgcatgg ccatcgctct gtttaaattg
     6421 agttttttta ttactcttgc aggtataatc ctcttattat tgctccctac tgtagccttt
     6481 gaatttttat tgggtcaggg tttccccgac atcaagtgga taattctctc actttcgcct
     6541 ggcattcttg ccatagcctc gggaatgatc ttcagtcatt tgttttcggg aaccggcaaa
     6601 cctcattata acatcattgg ctccggaata ggtttaatta ccctgattgc agccggatat
     6661 ttatttattc caaaattcgg acttatggct gctggcttgg taacctcatt ttcctatctt
     6721 tcttcattgg cttgccaaat atatcttttt agaaaaatgt cgggatatgc ttggagcgtc
     6781 tttatccctc gacgcagcga ttttgccttt ctttttacaa aaaccaaccg taaaccataa
     6841 tttgaaaatc atcagtctga tccttaaaaa cttttgcctt atttttcgtt agtttaaatg
     6901 cttgacaaac cttaacatta ttgagataat gaggaaacga ggagcctggg agactgttac
     6961 aagaagacca ggagaggtga caaggtgggt ggtggatgat gattggtggt ttataactgg
     7021 tggatgaatt tcgttaattg tcagttgttt gttgtttgtt tgtaatttgg agaccaaaga
     7081 ctgtgggaag ggtaaggtgg aatttataag ataatatgat aattttgcag aaattcgatc
     7141 gaaatgaaga ttattgagac cagaatacct ggtttattga ttgtaaagcc tgaggtattt
     7201 gaggatgaaa gaggttattt tttcgaatca tataatcagc agaaatttca agaattaggt
     7261 atagatgtag tttttgtgca agataatgaa tccaagtcgc agaaaaatgt aatacgtggt
     7321 ttacattttc aaaagcctcc tttcgctcaa gggaaactgg tgagggtaat gaagggttcg
     7381 gttttggacg tagcggtcga tttacgtaaa ggctctccta cttatggtca atgggattca
     7441 atcgtactta gcgaaagcaa taaattcatg tattggattc ccgaaggaat ggctcatggc
     7501 tttcgaaccc tgcaagacga taccttgttt ttctacaaat gcacgcattt ctataacaag
     7561 ggatcggaac aggctcttcg ctggaatgat cccgctttga acatcgattg gcagttaatg
     7621 gaaccaccca tactttccga aaaggacagg ctttcaccat tcttcaagga ttttgtcagc
     7681 cctttcgaat atgaatagga ccatatcttc attttatatt aattaatact catcaacctt
     7741 ctttttaaca atatcctgta tttttttctc ttttaaaatt tagctcttat tccagggggt
     7801 taatatttct actaattcta tatttaccac aattctttca acaatcaaaa gaatctcaga
     7861 tgagcacagg ttgaggatag gaaggaaatt tttaaaaact tataagaagg aaataaaaca
     7921 aagccttaca ttgtaggcaa agcctttagt agcggggacg agatttgaac tcgtgacctc
     7981 cgggttatga atccgacgct ctaaccacct gagctacccc gccttaaaaa gttcggcaaa
     8041 attagataaa ataatccagc ttgcaaacaa tcctccgctc acttctaatc ttttgctatg
     8101 ctctcagtgt ttttattctg acattttttt gagtgataat cacaccgaca attaccatta
     8161 acattcctac taaaatatta accgtcaatt tttcgtgaat cttgaaatag gaaaatatcg
     8221 ctgtaaatac tgggataaaa ttcgtaaaga tactcgtgcg gctcacacca agatggtgca
     8281 tgccaaaagt aaaaaagatg aatgcaagtg aagatgcaaa aacagcaagc atgatcagtg
     8341 ggtaccataa tccccactcg aaatgaagca aagtaagctg gctgggttcg actatcaata
     8401 ccaatgggag aaaataaaat gcgccaatga agtttaacca tgctacgatg gtgaaagaag
     8461 aataaagcat agtcagtctt ttcagcaaaa ctgcataaat taccgcagta gcaacagcaa
     8521 aaaacaaact aacaattcca attggattga attggaaaga aaaattgggg cctaacaaca
     8581 taaaaataat tcctcctaac gaaagaaaga atccaaaaat ttgcagtggt tgtaaaactt
     8641 ccttcagaaa aatagaagcc gcaatagccg aaaaaatagg aatggtagaa ataataatag
     8701 cacttatggt tggagtggaa tacttcactc catatccttc acccaggaaa tataagaatg
     8761 gttcaaacaa tgccgcaaat agaaataact tgtaatcttt ctttcgaaca ggttgcaatt
     8821 gatgagtaag tccgaaaata aaaaaaagta gagcactact gatgaccaat cgaagaaaaa
     8881 ttgtcgtaat aggatttaaa tatcgaaaca ccatggttgt ccatacatac gacatacccc
     8941 aaaaaaccat agctaatatc accaataaat agacaaaagc cgaaaattta ccatcttgtc
     9001 tcatccctaa gcttcttgag ttggtattgc aaaagtacaa acaccttcca gctgcatctt
     9061 gaaaaaattt aaaatcaagg gaaaaaaata tagttctcat gactttgact atggagatga
     9121 ggagacggaa ggaaaggaga atagagattg gggattggag attggagatt ggagattgga
     9181 gattggaggt tggaggttgg agattggaga ttggggattg gagattggag attggagatt
     9241 ggagattgga atttggagat tggaggttgg ggattggaga ttggagattg gagattggag
     9301 gttggagatt ggggattggg gattggagat tggagattgg aatttggaga ttggagattg
     9361 gggattggag gttggaattt gtttgcaatt tggaatttct aagttatcag ttgttagttg
     9421 taatttgcgt aaatcagcgg attaatctgc ggaaatcagc gagaaaaaat tttcctgatg
     9481 ttgacaaaag gaagggaaaa cagaggaagg gaggaaggga gattagagat tggaggttgg
     9541 agattgggga ttggggattg gaggttggag gttggagatt ggagattgga gattggagat
     9601 tgggggttgg aaattgaagg ttggagattg gaggttggag attggaggtt ggaggttgga
     9661 atttgtttgt aatttggaat ttctaagtta tcaagtgtta gttgtaattt gcggaaatca
     9721 gcggattaat ctggggaaat cagcgagaaa aaattttcct gatgttaact aaaggaaggg
     9781 aaaacagagg aatggaggaa gggagaatag agattggaga ttggaggttg gaggttggag
     9841 cttggtaatt gttggttgtt cgttgtcagt ttaaaaatac ttgacaaata gaaaaattat
     9901 ccttatcttt ctcctataaa tcctacccaa gttataccgc ctttggggaa gcgagttt
//
LOCUS       DAOORU010000090         9869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8497_length_9869_cov_4.754229, whole genome shotgun sequence.
ACCESSION   DAOORU010000090 DAOORU010000000
VERSION     DAOORU010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9869
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8497_length_9869_cov_4.754229"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..530
                     /locus_tag="PLF20_06695"
     CDS             81..530
                     /locus_tag="PLF20_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HOX80724.1"
                     /translation="MELKDIVAISGKTGLFKVVGQTKTGAIVESLIDGKRQPVFIHDR
                     LSSLADITVFTTEEDRPLRKILQAIRERENGGPAPSHKSDETTLKNYFATVVPDYDNE
                     RVYFSAIKKIISWYNLLHEKGLLDLLNEPEEKDISEENSTPVENPEQ"
     gene            613..1395
                     /locus_tag="PLF20_06700"
     CDS             613..1395
                     /locus_tag="PLF20_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOX80725.1"
                     /translation="MPGKFIHDFIVLRNISLNASHFILELLCPIQLPEIFPGQFVEVK
                     VEHSPATFLRRPFSIHEVDRQENYLQLLIQIKGDGTRQLSLLEPGESLNMIYPLGNSF
                     HMVKHEKVLLVGGGCGVAPLLFLARILKENENELYILIGAKTKEDILEKKKFEKFGKV
                     FITTEDGSEGRLGLVIHHGLWRQTPFMADKIYTCGPEPMMKEVARLALIHGINCEVSL
                     EQTMACGLGACLCCVVKTKDGHTCTCTQGPVFDSKELSGWVN"
     gene            1411..2328
                     /locus_tag="PLF20_06705"
     CDS             1411..2328
                     /locus_tag="PLF20_06705"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOX80726.1"
                     /translation="MADLTTQLHRLSLRNPVMTASGTFGYGEEFTDFIDLNHLGAIIV
                     KGTTATSREGNPYPRMAETASGMLNAVGLQNKGIDHFIHEIWPRLKQFKTPFIVNVSG
                     STIEEYIETAEKLDSLEGMPAIELNISCPNVKEGGMTFGTSCLAAASVTKAVRKVFHK
                     TLIVKLSPNVTDITEIAKAVEGEGADAVSLINTLLGMAIDAQSRKPILSTITGGLSGP
                     AIKPIALRMVWQVYHTVKIPVIGIGGIMNAMDAIEFLLAGAVAVQVGTANFIDPAVTL
                     KIIEGINDYCEQNNLSSIKDLIGALIIPE"
     gene            complement(2476..3345)
                     /gene="sucD"
                     /locus_tag="PLF20_06710"
     CDS             complement(2476..3345)
                     /gene="sucD"
                     /locus_tag="PLF20_06710"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOX80727.1"
                     /translation="MSVLVDKNSRVIVQGFTGNEATFHALQMIAYGTNIVGGVTPGKG
                     GTYHLNRPVFDTVKEAVNGTSANVSIIFVPPAFATDATMEAAEGGINLIVCITEGIPV
                     QDMIKVKEYIKAFGCRLIGPNCPGVITVGEAKVGIMPGFIHKRGVVGVVSRSGTLAYE
                     AVNQLTKVGLGQTTGIGIGGDPIPGTTIVEAVQLLMDDPETKGIVVIGEIGGTMEAEA
                     AYWIKDHGTKPVVAFIAGSTAPKGRTMGHAGAIIGGKDDTASAKKTILRQCGIFVVDS
                     PAEIGSRMLEILV"
     gene            4239..5777
                     /locus_tag="PLF20_06715"
     CDS             4239..5777
                     /locus_tag="PLF20_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOX80728.1"
                     /translation="MLVKTYGSAVIGVNALTITVEVDIEAGVNFFLVGLPDNAVKESQ
                     QRIEAALYNNELKYPGKKIVINMAPADIRKEGSAYDLTLAIGILAASEQVNANLLDQY
                     IIMGELSLDGELKPIKGALVIAIEARKQGYKGFILPSANAREAATVSDLEIIGAENLR
                     QVVDFLNGKIKIEPTVVNTREEFYQRLNEYEYDFADVKGQENIKRALEIAAAGSHNVI
                     LIGPPGSGKTMLARRLPSILPPLNLQEALETTKIHSVAGRLGCNASLISLRPFRAPHH
                     TISDAGLVGGGTFPQPGEISLAQNGVLFLDELPEFKRSVLEVMRQPMEDRVVTISRAK
                     HSVEYPASFMLVAAMNPCPCGYYNHPDIPCTCAPGQVQKYLNRISGPLLDRIDIHVEV
                     TPVPFRELAGQREGEKSAVIRKRVVGARKIQEERFKNYPNIHCNAQMTSKMIKTFCTI
                     PPEGQTILRTAMEKLGLSARAYDRILKVSRTIADLDDSPQIRTEHLAEAIHYRSLDRE
                     NWGK"
     gene            complement(5861..6757)
                     /locus_tag="PLF20_06720"
     CDS             complement(5861..6757)
                     /locus_tag="PLF20_06720"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HOX80729.1"
                     /translation="MEKLTSIMNRPIQRVAAIHDLSGFGKASLTVVIPILSTMGIQVC
                     PLPTAVLSTHTRFQGFHLIDLTPHLQTIIDHWKELHLHFDAIYSGFLGSHQQIKIVKN
                     FIDDFNTDKTLVVIDPVMGDNGHLYHSIQPEMITGMRSLVSKANVITPNITELSFLLD
                     KDYDPGISENKVKEYIRRMMDLGPETVIVTSVPQPDLPKRTSVIAYSRLDGRYWKVSC
                     DYLPASYPGTGDAFASIITGSLLQGDSLPIALDRAVQFLSYAVRATFGYQYNSIEGLL
                     LERVLQNLHAPVQISSYELLDE"
     gene            6864..8963
                     /locus_tag="PLF20_06725"
     CDS             6864..8963
                     /locus_tag="PLF20_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOX80730.1"
                     /translation="MKRLHTYLLSLLVLIAAGSVRAKEVPLNKAKQAAENQYHMQAGY
                     KGNIAETFEIKAKGQNVMYAFNFSEGGFVIISADDAFYPVIGYSLSGFYSEENQPEGF
                     RGMMADYRDQILYLKDLSIEATEEIAQIWTDLLSGQTLKRGGKAVGPLLPSQWNQDDP
                     YNYYCPADPAGPGGHVYVGCVATSMSQIMYYWRWPLQGEGSYTYYMPQYGYISANFGE
                     TQYNWNGMVYTSPVALNLPIALIGFHTGVSVHMDYSPTGSGAQSANVPDAAINYFRYN
                     SQIQYLQRIGYTSSVWENMVKEQMDNGYPVYYSGLEAGSNAGHAFVVDGYDDQTPRNF
                     HFNFGWDGYMDGYYTLANAGGFSTQQTMVRNFFPGTGYPYYSSAFTEIKDLVGTIEDG
                     SGPVADYLNNTDAKWLFNPQTEFDSVTNITISFYRFDLGTGDTLYIYDGEDENSLLIG
                     KYTGNNLPAAFTSSRNKVMIHFVSDGSVTSQGWLLNFKANLPVYCTGQKIFTQYDTTF
                     DDGSGRFYYNNNTSCTFKIVPPYASSVTVYFDYFDTEPENDWLKIYDLSTQQLLATYT
                     GSYPASSPPAPVTSPSGQVYMIWKTNSGINADGWQLHYETLNIGIESQGLLAECHVWP
                     NPASELLHLSFVVEKQQYINVQLFNATGSVVFSKDLKSFSGKYEEQIQVDKLAKGIYI
                     LKITGEKGIYNKKVVIQ"
     gene            complement(9167..>9869)
                     /gene="argF"
                     /locus_tag="PLF20_06730"
     CDS             complement(9167..>9869)
                     /gene="argF"
                     /locus_tag="PLF20_06730"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOX80731.1"
                     /translation="IYDGIEYRGYAQEIVETLARYSGVPVWNGLTNEFHPTQVLADLL
                     TMMEHCDKPLNKISFAYCGDARNNMGNSLMVGAAKMGMDFRAVAPKKLWQNEELTDIC
                     RKIAEETGARITLTENVDEGVKGVDFLYTDVWVSMGEPAEAWAERIHLLKPYQVNREL
                     VRKTGNPQVKFLHCLPSFHNRETSIGEEIYQKIGLESMEVTDEIFESPMSLVFDEAEN
                     RLHTIKAVMVATLGA"
BASE COUNT         2969 a         2077 c         2017 g         2806 t
ORIGIN      
        1 cggtcttgat tcttatttca gcgcaaggaa ttcagcgaaa tttcatgtaa ttttgcaagc
       61 ggaatcttaa attttggcaa atggaattaa aagatattgt agcaatttca ggaaaaacgg
      121 gtctttttaa agtagtaggc caaactaaaa cgggtgccat tgtagaatcc ttgatcgacg
      181 gtaaaagaca acccgtattt attcacgacc gtttgagcag tctcgctgat attactgttt
      241 ttaccaccga agaagaccgt cctttgagaa aaatcctaca ggccatcagg gaaagggaaa
      301 atggagggcc agccccaagc cataaatcag atgaaaccac cctaaaaaat tatttcgcaa
      361 ccgtagtacc tgattacgac aacgaacgag tctatttttc ggcgataaaa aaaatcatct
      421 catggtacaa ccttcttcac gaaaaaggat tactcgattt attaaatgaa cccgaagaaa
      481 aagatatttc tgaagaaaac tcaacaccag ttgaaaatcc agaacaataa tcttccaaaa
      541 atccgtcgat acaaaaaaga cggatttttt tatgttattt tgtaatcgaa gttccgttca
      601 tgaaaattta ttatgccagg caaattcatc catgatttta tcgttctaag gaatataagc
      661 ctgaatgcat cgcacttcat ccttgaattg ctttgtccta tacaactacc tgaaattttt
      721 ccagggcagt tcgtagaagt aaaagtggaa cattcaccgg ctacatttct aaggcgtcca
      781 tttagcattc atgaagtcga taggcaagaa aactaccttc aacttctcat acaaattaaa
      841 ggtgatggaa ctcgtcaact ttccttactc gagccaggcg aaagtttgaa tatgatttat
      901 ccattaggaa attcttttca catggtaaaa catgaaaaag tgctattggt tggaggagga
      961 tgcggtgtag ctcccttgtt gtttcttgca aggattttaa aggaaaacga aaacgagctc
     1021 tacattttaa ttggggctaa aacgaaagag gatatccttg aaaagaaaaa attcgaaaag
     1081 tttggtaagg ttttcatcac caccgaagat gggagtgaag gaaggcttgg tcttgtgatt
     1141 catcacggcc tttggaggca aacgccgttt atggcagaca aaatatatac ctgcggtcct
     1201 gagcccatga tgaaggaagt ggccaggctt gcgctaattc atgggataaa ctgcgaggtt
     1261 tcgctcgaac aaactatggc atgcgggctt ggtgcgtgtc tttgctgtgt cgtaaaaaca
     1321 aaggatggtc acacatgtac ttgcactcag gggcctgttt tcgattccaa agaactttcg
     1381 ggctgggtta actaatacat tcaagaagcc atggcagatt taactacaca actccaccgt
     1441 ctttcgcttc gaaatcccgt aatgactgct tcaggcactt ttggctatgg agaggaattt
     1501 accgatttca tcgatttgaa tcatttggga gcgatcatcg tgaaaggtac cacggcaaca
     1561 tcgcgtgaag gaaatcctta tccaaggatg gccgaaacag cctcgggaat gctcaatgca
     1621 gtaggtttgc aaaataaggg tatcgatcat ttcatccatg aaatatggcc tcgcttgaaa
     1681 cagtttaaaa cgcctttcat cgtaaatgtt tcggggagca ccatagaaga atacatcgaa
     1741 acagccgaaa aactggattc tctcgaagga atgccggcca tcgagctcaa tatctcctgc
     1801 cccaatgtca aagaaggagg catgaccttt ggaacttcgt gcctggccgc tgcgtctgta
     1861 accaaagccg taaggaaagt ttttcacaag actcttatcg taaaattgtc tcccaacgta
     1921 accgatatca ccgaaattgc aaaggcggtt gaaggtgaag gtgcagatgc ggtttcactc
     1981 atcaacactt tgctcggaat ggccatagat gcacaaagcc gcaaacccat tctttcgacg
     2041 ataaccggtg gattgagcgg acctgcgatt aaacccattg ctctgcgaat ggtatggcaa
     2101 gtttatcata ctgtaaaaat tcctgtcatt ggtattgggg gcattatgaa tgccatggat
     2161 gccatagaat ttcttcttgc aggagccgtt gccgttcaag ttggaacagc caattttatc
     2221 gatcctgccg taacactgaa gattattgaa gggataaatg attactgcga acaaaacaat
     2281 ttatcgagta taaaagattt gattggagcc ttaattattc ccgaataaac aacctttaaa
     2341 acaaattggt caaaacgctt ccctctcgat ttttcttcgt gagcaggatt ggaatgtacc
     2401 caaggcatta tttcctgata aatgagtagg aatgcgaaag aacctgttac atttcgattt
     2461 ttgattccaa atttcttaaa ctaaaatctc caacattctg cttcctatct ctgcggggga
     2521 atcgaccaca aaaataccac actggcggag gattgttttt tttgcactgg ccgtatcgtc
     2581 ttttcctccg atgattgcgc cagcatggcc catggttcgg cctttgggtg cggtggaacc
     2641 ggcaatgaag gccactacag gcttggtacc atgatctttt atccagtaag cagcttcggc
     2701 ctccatggtt cccccaattt ctcctatcac tacaattcct ttcgtctcgg gatcatccat
     2761 gagcaattga acggcttcta caatggtcgt tcctggaata ggatcgccgc ctatgccaat
     2821 acccgtcgtc tgccctaatc ccactttggt taattgattc actgcttcgt aagccaaagt
     2881 accactacgg gaaacaacgc ctaccacacc tcttttgtgg ataaaacctg gcatgattcc
     2941 tactttggct tcacccacag tgatgactcc tggacaattc ggcccaatga gcctgcaacc
     3001 aaaagccttg atatattctt taaccttgat catgtcttgt acaggaattc cttcagtaat
     3061 acatacaatc aggtttattc caccttcagc agcctccatg gtggcatcgg tggcaaaagc
     3121 tggtggaaca aaaattatag aaacattcgc cgatgtgcca ttcacagctt ccttcacggt
     3181 atcgaaaaca ggacgattca aatgataagt ccctcctttg cccggagtta ctccccctac
     3241 gatattcgtc ccatatgcta tcatctgcaa agcatgaaaa gtggcttcat tgcccgtaaa
     3301 tccttgtaca ataacccttg aattcttgtc aactaataca ctcattgtaa attaagaatg
     3361 tcgatatctg aaacaaaact tgactttctc cctcttcaaa tccaattacg aaaagagact
     3421 tcgaaaaaag agctataaat atgaacaaac aactcgaaaa aagtttggcc aaattataaa
     3481 aattcacaca aagataaaac aacccaggtt taaaatgata aagcctcaac gataaaacaa
     3541 gaaatcgctg aattcatcat gaaaatttcg ttcggctcta gccctgtgca tcagccttaa
     3601 ctatatttac aggctttgta tatcgaacaa atatcactct actattctcc agttcaaatc
     3661 tattttagat acttaaaaca tattgattcc catgctactc gaaaaccagc tccgtccaca
     3721 tcgtaggaat ttacaagcct ttatctgaaa tctataaaaa atgcttactg gcatgatttt
     3781 cgaaatcaag aacagctcga tctaaatttc ctctcctgct tgatatcagt attagtaaat
     3841 gtatgtggta cagcatgatt acacaaacca ggcaagatat aaacccttat tcgcttcata
     3901 atacattagg ttttcaaaat ctatatcagg gtataccctg cgtaaaaaga tgatccattc
     3961 agccaaacct tcaaaacgtc agaatgtaat ccgttttctc tgttatttta tagctttgcc
     4021 gatatatacg aacgaattca cactttcttg tgaacatgca aggccttgtc gatattatcc
     4081 atttctgagg tgtcagctgc taaaatacat cgattcaggc gatagtctaa gccgaactat
     4141 aaagaggatc aagtttgaaa ttcgtcgata agattaatag caaaaacatt ttactatctt
     4201 taccaaatca ttaattttaa aaaccagcct gagagtttat gcttgtaaag acttatggga
     4261 gtgcagtaat tggggtaaat gcgctgacga taactgttga ggtagatatc gaagcggggg
     4321 tgaatttttt tctggtaggt ttacccgaca atgcggtaaa ggaaagccag caaagaatag
     4381 aagcggcctt atataataat gaattgaaat acccgggtaa gaaaattgta atcaatatgg
     4441 cgccagccga tattcgaaag gaaggatcgg cttatgattt gactctggcc ataggcatac
     4501 ttgcagccag tgaacaggta aatgcgaatc tactcgacca gtacatcatc atgggggagc
     4561 tatcgttaga tggtgagtta aagccaataa aaggggcgct ggtaatagcc atagaagccc
     4621 gaaaacaggg gtacaagggt tttatccttc cgtcggccaa tgcccgtgag gcagccacgg
     4681 taagcgatct tgaaatcatt ggtgccgaaa acctccgaca ggtagttgat tttttgaatg
     4741 gaaagataaa gatcgagccc acggttgtga atacgcggga agagttctat cagcgcttga
     4801 atgaatatga atacgatttt gctgacgtaa aaggtcagga aaatataaag agagccttag
     4861 agatagcagc ggccggaagt cataatgtga ttttaattgg tcctccgggc tcgggtaaga
     4921 ccatgttagc tcgccgattg ccttccattt tgccacccct gaacctgcag gaagcgctcg
     4981 aaaccactaa aatccattcc gtcgccggac gtttaggatg taatgcctct cttatttcgc
     5041 taaggccttt cagggctcca catcatacca tttcagatgc aggactggtg ggtgggggga
     5101 ctttcccaca acctggagaa atttctctgg cgcaaaatgg agtcttgttc ttagatgaac
     5161 ttcccgaatt caagcgtagc gtcctcgaag tgatgcgtca gcccatggaa gaccgtgtag
     5221 ttaccatctc aagagctaaa cattcggtag aatatcctgc gagttttatg ctggtagcag
     5281 ccatgaatcc ctgtccatgt ggatattata accatcctga cattccctgc acctgcgcgc
     5341 cgggacaagt acagaaatac ttgaatagaa tttcgggacc tttgttagat agaatcgaca
     5401 tccacgtaga agtaactcca gtacctttta gagagcttgc aggacagcgc gaaggagaaa
     5461 aaagtgcggt cattagaaaa cgtgtagtcg gcgccagaaa aattcaagaa gaaagattca
     5521 agaattatcc gaatatccac tgcaatgcac aaatgacttc taaaatgatc aagacttttt
     5581 gtacgattcc acccgaaggt caaactatac ttcgcactgc catggaaaaa ttaggtcttt
     5641 ctgcaagagc ctatgatcgt atcctcaagg tatcacgcac catagccgat ctcgacgata
     5701 gtccacagat tagaaccgaa cacctagcag aggccatcca ttacagaagc ctcgatcgcg
     5761 aaaattgggg gaaatgaaga caaaaccttt atttgtgcaa attgaattcc tatatcatac
     5821 aaaccattac cctgtctatg gtattctaat tcaatgaacc ctattcatcg aggagttcat
     5881 agctgctaat ttgcactgga gcatgaagat tttgcaaaac ccgttcaaga agcagtcctt
     5941 caatcgaatt atattgataa ccaaaagtag ctctgactgc ataagacaaa aattgaacag
     6001 ctcggtcgag agcgatggga aggctatccc cttgaagaag cgagccagtg atgatactgg
     6061 caaaagcatc gcccgtacct ggataactgg ctggaaggta atcacaactg actttccaat
     6121 atctgccatc cagccgacta taagcaataa ctgatgtcct ttttggcaaa tctggttgag
     6181 gtacactggt aacaattacc gtttcaggac ctaaatccat cattcgccga atatattctt
     6241 tcactttgtt ttcacttatc ccagggtcgt aatctttatc gagcaaaaaa gaaagttcgg
     6301 taatattagg tgtaataaca ttagccttcg agacgaggct tctcattcca gtgatcatct
     6361 caggctgtat tgaatgatat aaatgcccat tgtctcccat cacgggatca ataaccacca
     6421 atgtcttatc tgtattgaaa tcatcaatga aattttttac gattttgatt tgctggtgcg
     6481 atccgaggaa tccgctataa attgcatcaa aatgcagatg gagttctttc cagtgatcga
     6541 taatcgtttg aagatggggt gtcaaatcaa tgagatgaaa tccttgaaat cgggtatgtg
     6601 tggataaaac ggcagtaggc aaagggcaaa cctgaatacc catggtagaa agaatgggaa
     6661 tcactactgt cagggaagcc tttccgaatc ccgaaagatc atgaattgcc gctactcgct
     6721 gaataggcct gttcataata gatgttaatt tttccacaaa tataaatttt atgtcatgta
     6781 aattaatgtc caatttctcc acaagtgaat ttgttttatt tttgtgctcc tattattgtt
     6841 gtacaattta atttttttat tggatgaaaa gattacatac ttacttactc agtttattag
     6901 tattgatagc agccgggtct gttagagcaa aagaagtacc gttgaataag gcaaagcaag
     6961 ctgcagagaa tcaatatcat atgcaggccg ggtataaggg aaacattgct gagactttcg
     7021 aaatcaaggc aaaggggcag aatgtgatgt atgcttttaa tttcagtgaa ggcggatttg
     7081 ttatcatctc ggcagatgat gcattttatc ctgttattgg ctattcgctg tcaggatttt
     7141 acagcgaaga aaaccagccg gaagggtttc gagggatgat ggccgattat agagatcaga
     7201 ttttatatct gaaagatttg tcaatcgaag caactgaaga gattgctcaa atttggacag
     7261 atttattatc aggtcagact ttgaaaagag ggggcaaggc ggttggacct ttattgccca
     7321 gtcagtggaa tcaagatgat ccgtataatt attattgtcc ggccgatcca gctggaccgg
     7381 gtggacacgt ttacgtgggt tgtgtagcca cttccatgag tcagattatg tattactgga
     7441 gatggccttt gcagggagaa ggttcctaca cctattacat gccacaatat ggatatatct
     7501 cagccaattt tggagaaaca caatacaatt ggaatggcat ggtatatact tcgcccgttg
     7561 ctcttaattt acccattgct ttaatcggtt tccatacggg agtatcggtg cacatggatt
     7621 acagcccaac aggttcgggt gcgcaatcgg ccaatgtccc cgatgctgct ataaattact
     7681 tccgttataa tagtcagatt caatatttgc agcgaattgg ctatacttcg tcggtatggg
     7741 aaaatatggt aaaagaacag atggataatg gctatccagt atattattct ggtctcgagg
     7801 caggttcaaa tgctggccat gcttttgttg tggatggcta tgatgaccaa actccccgta
     7861 attttcattt caattttggt tgggacggtt atatggatgg atattatact ttggcgaatg
     7921 ccggtggttt ttctactcag cagactatgg tgcgtaactt ctttcctgga accggttatc
     7981 cttactattc atctgcattc accgaaatca aggatctggt tgggacaata gaagatggtt
     8041 ctggcccggt ggcagattat ctgaataata ctgacgctaa atggcttttc aatccgcaaa
     8101 cagaatttga ttccgtaacc aatattacca tatcattcta ccgtttcgat ttaggaaccg
     8161 gcgatacgct atatatctat gatggagaag atgaaaattc tcttctcatt ggaaaatata
     8221 caggcaataa cttacctgct gcatttactt cgagccgtaa taaagtgatg attcacttcg
     8281 tttctgatgg atctgttact tctcaaggat ggctcctgaa ttttaaagct aatcttcctg
     8341 tgtattgcac gggtcagaaa atcttcaccc aatatgatac taccttcgac gatggaagtg
     8401 gccgctttta ttacaataac aatacaagct gtacatttaa gattgttcct ccttatgctt
     8461 cctctgtaac tgtatatttt gattatttcg atactgaacc agaaaacgat tggttgaaaa
     8521 tttacgacct atccacacag caattgctgg caacttacac agggagttat cctgcttcca
     8581 gtccaccagc acccgttact tcgcccagtg ggcaagtcta tatgatttgg aaaaccaata
     8641 gtggtatcaa tgccgatgga tggcaattgc attatgaaac cttgaacata ggaatagaat
     8701 cccaaggact tctggccgaa tgtcatgttt ggcctaatcc agccagtgag ttacttcatc
     8761 tatcctttgt ggtcgaaaaa cagcaatata taaatgttca gttatttaat gccacgggat
     8821 cagtggtatt ttcgaaggat ttaaagagtt tttcgggaaa atatgaggaa cagattcagg
     8881 tcgataaatt agctaagggc atttatattt tgaaaatcac tggagaaaaa ggaatatata
     8941 acaagaaagt agtcattcaa taagggaagt ctgaattata gtttcttttg cggccgccac
     9001 tgcgtggcgg ccgcatcttc tacaacccca tccccctcaa tcatcagaaa tttatatccc
     9061 taaagaaaac cggctcgcct attatgcgat ccggttttct tttaaatcat cgaagctcgt
     9121 cgaaaaaacg aagatactgc ttcctgtttt ggttagtgac gtattactaa gctcctaaag
     9181 tagctaccat aactgctttt atcgtatgaa gacgattttc ggcttcatca aaaaccaatg
     9241 acatgggcga ttcgaatatt tcatcggtaa cctccatgct ttccagacca attttctgat
     9301 aaatttcttc acctatactc gtctcacggt tatgaaacga aggaaggcaa tgtaaaaatt
     9361 ttacctgagg attacctgtt ttcctcacta attccctgtt aacttgatat ggtttcaaca
     9421 aatgaatacg ttcggcccaa gcttcagcag gttcacccat cgagacccaa acatctgtat
     9481 agagaaaatc tactcctttt acgccttcat ccacattttc ggtcagcgtg attcgggcac
     9541 cggtttcctc ggcaatttta cgacaaatgt ctgtcaattc ttcattctgc cacaattttt
     9601 taggcgctac tgccctgaaa tccattccca ttttggctgc tcctaccatg agcgaattac
     9661 ccatattatt tctggcatct ccacaataag caaacgaaat tttattcaat ggcttgtcgc
     9721 aatgctccat catagtgaga aggtcggcca gaacctgagt gggatgaaat tcattggtca
     9781 aaccattcca aaccggaact cccgaatatc ttgccagagt ttccacgatt tcctgtgcat
     9841 atccccggta ttctattccg tcatatatt
//
LOCUS       DAOORU010000091         9758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8651_length_9758_cov_4.644234, whole genome shotgun sequence.
ACCESSION   DAOORU010000091 DAOORU010000000
VERSION     DAOORU010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9758
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8651_length_9758_cov_4.644234"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="PLF20_06735"
     CDS             complement(<1..441)
                     /locus_tag="PLF20_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80732.1"
                     /translation="MKIKKLLEFTGWAVAVAGTSFLLSFANSEQNKTVCKDIIIQLHQ
                     GDYALVSENEILGKLTKNYGNLQGKPIHKIPFRNIESEIMKISALSRADVFATPSGYV
                     KISAVERMPLIKIFNHQNKNFYLDKEGNIFTTQTSHSARVLIANG"
     gene            complement(438..1805)
                     /gene="murC"
                     /locus_tag="PLF20_06740"
     CDS             complement(438..1805)
                     /gene="murC"
                     /locus_tag="PLF20_06740"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOX80733.1"
                     /translation="MKPDELKSVYFLGIGGIGMSSIARYFHQKGISVSGYDRCPSRLT
                     EELMAEGIEIHFEERPACIPSEIELVIYTPAIPADNLEFRYAAEKGIPMMKRSQVLGL
                     LTCEKPTLAVAGTHGKTTISTMAAHILFNSHLGCTAFLGGISKNHQSNFITKNGSNWV
                     VIEADEYDHSFLELKPQIAIITAIDPDHLDIYHDYQNLVNAFKDFAGLISANGLLILK
                     KGLENLLSGSYLCRKATYSFNDTSADYFARDILLEKMKYSYSIQTPEGIIGPVQLKVP
                     AYVNIENSIAAFAAALAAGCSAEEIIRALSDFEGIRRRFDILYQSDNFTLIDDYAHHP
                     EEIKALAISLRNLFPTKKLTAIFQPHLYSRTRDLAQGFAQSLSLFDEVFLMDIYPARE
                     HPIHGVDSQMIMKKMSQVAHLVTREEAFREIISRKHQVLATIGAGDIEKLALLAKENL
                     NRQKS"
     gene            complement(1802..2905)
                     /gene="murG"
                     /locus_tag="PLF20_06745"
     CDS             complement(1802..2905)
                     /gene="murG"
                     /locus_tag="PLF20_06745"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOX80734.1"
                     /translation="MNILISGGGTGGHIFPAIAIAKALQKLRPDADILFVGAKGKMEM
                     EKVPQAGFIIKGLWISGFSRSFSISNLLFPLKLMVSSISAYYILRRFRPVVVIGVGGF
                     ASGPVMRVASYLKIPIVIQEQNSYPGVTNRILAKKASRIFTAYPNMEAFFPAEKTLLT
                     GNPVRKEIINTEHKRPEAIAYFELKEKVTTILVIGGSQGALSINEAVKTCLSLRHTQW
                     MWQTGRIYFEKAFAFINENHLENVKTRAFIERMDLAYAAADIIISRAGAIAISELCII
                     GKPTILVPYPYAAANHQMHNARRLEAAGAAIIIKDDELPLALPFTLEKLLDDTHWQEN
                     LSTQIRKWSIPDADERIAREIIQMVENSKPAKP"
     gene            complement(2902..4140)
                     /locus_tag="PLF20_06750"
     CDS             complement(2902..4140)
                     /locus_tag="PLF20_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOX80735.1"
                     /translation="MAEITKKISGDRVIWVMVILLAAFSILAVYSSTGSLAYKKQNGN
                     TEFYLLKHGGIILVGFILMYLTYRIPYHYFSKYALYGLIGAIALLIYAIINGKTINES
                     YRFIEIPMTHITFQPSDIAKVALYAYIARILNQKQEVLPKFKEGFWHLLWPVGLVCLL
                     VLPGNFSTAALIFLVAFAMFIVGNAYWKHLLILLLSGLILAVSVITIGQEFPNLLPRA
                     TTWSDRIVDFVKGDELDAVERLNDPDYQQSEQAKIAIATGGLFGKAPGKSIQRNFLPS
                     AFSDFIWAVIIEEYGLILGGISLMMIYLIILYRGIKIALACPTIFGTLLAFGITFSII
                     LQAFVNTAVAAGLMPVTGQTLPMVSMGGTSIWFTCIMFGMLQSIARGDKKIDIVQEEK
                     LVENTEEKIEENICMEEVLA"
     gene            complement(4181..5521)
                     /gene="murD"
                     /locus_tag="PLF20_06755"
     CDS             complement(4181..5521)
                     /gene="murD"
                     /locus_tag="PLF20_06755"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOX80736.1"
                     /translation="MNKYPIVVLGAGESGAGAASLARTEGYGVFVSDLGTIQPYYKSI
                     LQKRNIPYEENRHSPEIILYADEIIKSPGIPDNTPIVLEAKQKGIPVISEIEFAARFS
                     NGRFICITGSNGKTTTALLTHHILSNGSIDAGLAGNVGKSFAMELLKGDHEWWVLEIS
                     SFQLDGMFKFKADIAILTNITPDHLDRYANFEEYTASKMRIIQNQGPTDAFIWFANDP
                     VIWSEVDKRNLTQRIIPYGEKALGYDEECWIENNKIKIKINQYIMSITIENLALQGRH
                     NTHNSMAAAVAAKLLDVRSRKIKESLSDFKNIEHRLEFVASVHGIDFINDSKATNVNS
                     TWYALESMKKPVIWIAGGKDKGNDYSILIDLVKQKVKAIVCLGVDNSKIRRTFAGLVD
                     TIVEAYSADQAVEAAYRLGQNGDVVLLSPACASFDLFENYEERGKKFKQAVKRL"
     gene            complement(5527..6780)
                     /gene="mraY"
                     /locus_tag="PLF20_06760"
     CDS             complement(5527..6780)
                     /gene="mraY"
                     /locus_tag="PLF20_06760"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOX80737.1"
                     /translation="MLYYLFTYFDLAFDFPGAGLFQYISFRAASAVILSLIITLIFGG
                     TIIQYLKKRQIGEEIRDLGLEGQLQKRGTPTMGGLMIIAGIVIPTLLFARLDNVYIIL
                     MIITTLWLGFIGFVDDYIKVFKKNKEGLQKRTKLAGQVILGIVVGVTMYFSPDIVIRE
                     KTETHAFLETTNVLRESNYERAQEKFTAESTKSLKTTIPFVKNNEFDYAVLVNWIGKE
                     ARSYAWLVYIPIIILIITAVSNGVNLTDGLDGLAGGTSAVAASTLGILAWVSGNIIFA
                     DYLNIMYIPNTGELAIFISSLVGATIGFLWYNTYPAQVFMGDTGSLTLGGIIAVIAII
                     IRKELLIPILCGIFLAEALSVLLQVTYFKYTKRRFKQGRRIFKMAPLHHHYQILGYHE
                     AKIVQRFMIVSFMLAVITIITLKIR"
     gene            complement(6781..8250)
                     /locus_tag="PLF20_06765"
     CDS             complement(6781..8250)
                     /locus_tag="PLF20_06765"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004341558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOX80738.1"
                     /translation="MNKTLKDIIVNISAESIIGVLDQPVNRVVLDSREANEGSVFVAI
                     KGQKTDGHEFIEKAIENGATVIVCQSLPIVLDPRVTYLQVKNTSLAVGPIASAFFDYP
                     SRRLKLVGVTGTNGKTTITTLLYRLFKSLGYTSGLISTIENRIDNEILPAHLTTPNAV
                     SLNALLNSMAKAGCSHVFMEVSSHAIAQGRIYGQEYTGGIFTNLTHDHLDYHKTFSEY
                     LNAKKMFFDNLGNTAFALSNADDPNGEVMLQNTKARKYFYGLKNLADFHSRILEMDLE
                     SMLLHIDGYETWFRLTGRFNAYNLTATYGAAILLGETSEEVLRVMSNLHPAEGRLDIL
                     KSEEGINVIVDYAHTPDALQNVIETVRDIHAGAGRLIVVCGAGGDRDKSKRPKMAAIA
                     AENADHLILTSDNPRSEDPLQIIEDMKAGLNPSLSYRVLSIPDRREAIHIACMIAKTG
                     DVILVAGKGHEKYQEINGVKYPFDDKEILSQYLHLIKPR"
     gene            complement(8252..>9758)
                     /locus_tag="PLF20_06770"
     CDS             complement(8252..>9758)
                     /locus_tag="PLF20_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HOX80739.1"
                     /translation="MQKISGGEWIPVMGAQRIEPRNGKDIVTTIDIKLQDVAEQALLE
                     QLTENDAYQGCVVLMEVATGDIKVIANLRKIKDGSYDETYNYAIAESIEPGSIFKLPS
                     LVAVMEYAGYNIKDSVSYQGSISWSRNAVMKDDHPLNRTYITLEDAFAHSSNVGVSKI
                     VYKAFGKKPKRFSNALYNMGLGKPLGIEIPGEGEPFIRKADDPNWSKLSLPYMAIGYE
                     LKITPLQILTFYNAIANNGIMMKPRFVKQIQESGITVKQYEPQVLIKEVCSQKTALKA
                     REMMKAVTEKGTARGAFNGAPYKVAGKTGTAQIVTSKKYNKENYNASFVGYFPADNPK
                     YSLIVIVSNPSKGKIYGGAVAAPVFRAIADQIYATQLGVQDASTAYTSEFVTKNDSIS
                     QQHVSMIGWLPDLLQVNRLLGFNCDTLELTNEFGYCERHNHRVSTKILSFSDKSIPNL
                     KGMPAKDAVYLLESMGLKVILEGKGKVNAQSLPAGTQVKTGSVITLTLSHS"
BASE COUNT         2675 a         2080 c         2013 g         2990 t
ORIGIN      
        1 gccgtttgct atcaatactc tggctgaatg tgaggtttgc gtggtaaaga tgttcccttc
       61 cttgtctaaa taaaaattct tattttggtg gttgaaaatt tttatgagcg gcattctttc
      121 tacggctgaa atttttacat agcccgaagg agttgcaaaa acatcagccc tcgataaagc
      181 ggatattttc attatttctg attctatgtt tcggaatgga attttatgga taggctttcc
      241 ttgtaaattg ccgtaatttt tggtaagttt tcctaagatt tcgttttcag aaacgagcgc
      301 ataatcgcct tgatgaagct gaataatgat atccttgcat acggttttat tttgctcgga
      361 attagcgaat gaaagcaaga aggaagttcc agccactgcc accgcccatc cggtaaattc
      421 aaggagtttc ttaatcttca tgatttttgt cggttaagat tttctttggc aagcaaggct
      481 aatttttcga tatcacctgc accaattgtt gccaacacct gatgtttgcg gcttatgatt
      541 tctctgaatg cttcttcacg ggtaactaaa tgtgccactt ggctcatttt tttcataatc
      601 atctgggaat ctacaccatg aatagggtgt tcacgtgcag gataaatatc catgagaaat
      661 acctcatcga aaagagacag actttgtgca aaaccttgag ctagatctct ggtacgacta
      721 tagagatgag gttggaaaat ggcagttaat tttttcgtgg gaaataaatt ccgcaaagat
      781 atggccaatg cctttatttc ttcgggatga tgcgcatagt catcaatcag ggtgaaatta
      841 tccgactgat aaagtatatc gaatcgacga cgaattcctt cgaaatctga caaagcccga
      901 ataatctctt ctgcactgca tcccgctgca agggctgcag caaaggctgc aatactgttt
      961 tcgatattta cataagcggg cacttttaat tgtactgggc caataattcc ttcgggtgtt
     1021 tgtatagagt aggaatattt cattttctcg agcaaaatat ctctggcaaa ataatcggcc
     1081 gaagtatcat tgaatgagta agtcgctttg cggcataaat aactgccaga tagaagattt
     1141 tcgagtcctt ttttcaggat aagcaatcca ttggcagata tcaggcccgc aaaatcctta
     1201 aaagcattca caaggttctg ataatcgtga tatatatcca gatgatcggg gtcgatagcc
     1261 gttattatgg caatttgtgg tttcaactca aggaacgagt ggtcatattc gtcggcttcg
     1321 attaccaccc aattgcttcc attttttgta atgaaattcg attgatgatt tttgcttatg
     1381 cctcccagaa acgccgtaca acccagatgt gaattaaaaa ggatatgggc cgccattgta
     1441 gaaatagtgg ttttcccatg tgtacctgca actgccagtg taggtttttc gcaggtaagt
     1501 aatcccagaa cctgtgatcg tttcatcatg ggaatgcctt tttcggcggc ataacgaaat
     1561 tcgaggttat cggcagggat agccggagta tagattacca attcgatctc ggaaggtatg
     1621 caagcggggc gttcttcgaa atgaatttct ataccttcgg ccataagttc ttcggtgagg
     1681 cgtgaagggc atcggtcgta gcccgataca cttattcctt tttgatgaaa gtatcgggca
     1741 attgagctca tgccaatacc tcctatgccc aggaaatata ccgattttag ttcgtcaggt
     1801 ttcatggctt tgcaggtttt gaattttcga ccatttgaat aatttctcgg gcaattcgtt
     1861 cgtcagcatc tgggatactc catttgcgga tttgagtgga tagattttct tgccaatggg
     1921 tatcgtcgag gagtttttcg agagtaaagg gcagagcaag ggggagttcg tcgtctttga
     1981 tgatgatggc tgcgcccgcc gcctcaaggc ggcgggcatt atgcatctga tgattggctg
     2041 cagcgtatgg atacggaacc agtatagtcg gcttcccaat gatacataat tcgcttatag
     2101 ctatggcacc agccctcgaa ataataatgt cagccgctgc ataggctaga tccatccttt
     2161 ctataaaggc tcttgtcttt acattctcca gatggttttc gtttataaac gcgaaggcct
     2221 tctcgaaata aattctgccc gtttgccaca tccactgggt atgtctcagc gaaagacagg
     2281 ttttaactgc ctcattgatg gataaggcac cctgacttcc tccaatcact aaaatcgttg
     2341 taaccttctc tttcagttcg aaataagcaa tggcttcagg tcgcttatgt tcggtattaa
     2401 taatttcctt gcgtacagga ttaccagtaa gcaaggtttt ttcagccggg aaaaatgctt
     2461 ccatattggg ataagccgtg aaaattcgtg aggctttctt cgcaagtatt ctattagtta
     2521 cccctggata ggaattttgt tcttgaatta caatgggaat cttaagataa gaagcgactc
     2581 gcatgactgg tccacttgca aatcctccaa cacctatcac tacgaccgga cgaaaccttc
     2641 gaagaatata gtaagcactt atgctgctta ccattagttt caaagggaaa agcaagttag
     2701 aaatcgaaaa tgaacggctg aaaccactaa tccacaatcc tttgatgatg aaacctgctt
     2761 gaggcacttt ttccatttcc attttgcctt ttgccccaac aaaaagaatg tcggcatcag
     2821 gccttagctt ttgcagagcc tttgcaattg caatggccgg aaaaatatga cctccagttc
     2881 ctccaccact tatcaggata ttcatgccag tacttcctcc atgcaaatat tttcttctat
     2941 tttctcttcc gtattttcta caagtttttc ttcctgtacg atatctatct ttttatcgcc
     3001 tctggcaata ctttgcaaca ttccaaacat gatgcacgtg aaccagatag aagtgcctcc
     3061 catactgacc ataggcagag tttgccctgt tacgggcatc aagcctgctg caaccgccgt
     3121 attgacgaat gcttggagaa tgatgctaaa agtgattcca aaggccagta atgtcccgaa
     3181 tattgtggga caagcaagag caatctttat ccctctatat agaatgatca gataaatcat
     3241 catcaaactt atgcccccta aaataagtcc atattcttcg atgatcacgg cccagatgaa
     3301 gtcggaaaag gcactgggaa gaaaatttcg ctgaattgac ttccctggcg ccttgccgaa
     3361 taatcctcct gtggcgatgg caatcttggc ttgttccgat tgttgataat caggatcgtt
     3421 aagacgctca acggcatcca attcatcgcc ttttacaaag tcgacgatac gatcgctcca
     3481 tgttgtggct cttggtaaaa gattgggaaa ttcctgaccg atcgtaatta ccgatacagc
     3541 caaaatcaac cccgatagca ataagatgag aagatgtttc cagtaagcat ttccgacgat
     3601 aaacatggca aaagcaacca gaaatattaa agcagcagtg gaaaaattgc ccggcaacac
     3661 gaggagacat acaagcccca ccggccatag caaatgccag aatccttcct tgaattttgg
     3721 aagaacttcc tgtttttgat tcaatattcg tgcaatatag gcatatagag ccaccttggc
     3781 aatatcactg ggttgaaaag taatatgtgt catgggaatt tcgatgaaac gataggattc
     3841 attaatggtt ttcccattta tgatggcata aataagaagg gcaatagcac ctatcaaacc
     3901 atataatgca tatttcgaaa agtaatgata gggtatccta taggtgagat acattagaat
     3961 gaatcccacc agaataatgc ctccatgttt gaggagatag aattcagtat taccgttctg
     4021 ttttttataa gccaacgaac cggtcgagct gtacactgcc aaaatggaaa acgctgccag
     4081 cagaatcacc attacccaga taactctgtc tcctgatatt ttttttgtaa tttctgccat
     4141 tttattgcct tcttattttg cttataacat aacatgctaa ttaaagcctt tttacggcct
     4201 gtttgaattt tttacctctt tcttcataat tttcgaaaag gtcgaaactg gcgcaagctg
     4261 gcgaaagcaa aaccacatcc ccattttgtc cgaggcgata tgctgcttca actgcctgat
     4321 ctgcagaata ggcctcgact atcgtatcga ctaatcccgc aaaagttctt cggatttttg
     4381 aattatccac acctaaacac acgatggctt taaccttttg ttttactaag tcaatgagta
     4441 tggaataatc atttcccttg tctttcccgc ctgctatcca tatcacgggt tttttcatac
     4501 tttcgagggc ataccaggtc gaatttacat tggtagcctt tgagtcgttg ataaaatcaa
     4561 tgccatgaac actcgctaca aattcgaggc ggtgttctat attcttgaaa tccgacaggc
     4621 tttcctttat cttgcggctg cgcacatcca gcaactttgc agctacggct gcagccatcg
     4681 aattatgcgt attatgcctg ccttgcaatg ccaggttctc gattgtgata ctcatgatgt
     4741 attgattgat ttttattttt atcttgttgt tttcgatcca gcactcctcg tcgtaaccca
     4801 aagccttttc accataagga attattcgct gagtgaggtt tcgcttatcg acctctgacc
     4861 aaattacagg atcattggca aaccagatga aggcatcagt gggtccctga ttttgaataa
     4921 tacgcatctt cgaagccgta tattcctcga agttggcata ccggtcgaga tgatcgggag
     4981 taatattggt tagaatggct atgtcggctt tgaacttgaa catgccatca agctgaaaac
     5041 tactgatttc gagcacccac cattcatggt ccccttttag caactccatg gcaaagcttt
     5101 tacccacatt acccgccaaa cctgcatcaa tacttccatt gctgagaata tgatgcgtga
     5161 gaagcgcagt ggttgttttt ccgttgctgc cggtgataca aataaacctt ccattactga
     5221 aacgtgcagc aaattcgatc tccgaaatca cgggtatccc tttttgtttt gcttctaaaa
     5281 ctataggggt attatctgga attcctggac tcttgataat ttcgtcggcg tagagaatga
     5341 tttccggcga atgccggttt tcttcatagg gaatattcct tttttgcaag atggatttat
     5401 aatatggttg aatagttccc aagtcggaaa caaatactcc ataaccttca gttctggcca
     5461 aagaagctgc accggctccg ctttctcctg ctcctaaaac tactatgggg tatttgttca
     5521 tattgtttat cgaattttaa gggttataat ggttattacg gcaagcatga atgaaacgat
     5581 cataaagcgt tggacaattt tagcttcgtg atagcccaga atctgataat gatgatgaag
     5641 tggggccatt ttaaagattc tgcggccttg tttgaatcgg cgtttagtgt atttaaaata
     5701 ggttacctga agcaaaaccg acagggcctc ggccagaaaa ataccacata agatgggaat
     5761 taacagttct ttacgaataa taatggcaat tactgcaata attccaccca gagtgagcga
     5821 gccagtatct cccataaata cctgagccgg ataggtgttg taccacaaaa agcctatcgt
     5881 agcacctacc agagaactga tgaaaatagc cagctctcct gtattgggta tatacatgat
     5941 attgagataa tcggcaaaaa tgatatttcc cgagacccac gcaagaatac caagagtgga
     6001 ggcagccaca gccgaagtgc cgcctgccag gccatccaaa ccatccgtga gatttacgcc
     6061 gttcgatacc gcggtgatga tcagaattat gatggggata tataccaacc aggcatagct
     6121 ccgtgcttct ttgcctatcc agttcaccaa tacagcataa tcaaattcgt tgttttttac
     6181 aaaaggtatg gttgttttca gcgatttggt agattcggca gtaaattttt cttgggcacg
     6241 ttcataatta ctctcacgca gaacattggt agtttccaga aatgcatgag tttcagtttt
     6301 ttcacgtatc actatgtcag ggctgaaata catggtaact cccactacta ttcctaaaat
     6361 tacctgtccg gcaagcttgg ttcttttctg aagaccctcc ttgtttttct tgaatacctt
     6421 aatataatca tctacaaatc cgatgaaacc aagccataat gtagtgatta tcattaagat
     6481 aatatagaca ttgtctaaac gtgcaaagag cagggttgga ataacaattc cagcaataat
     6541 cattaaaccg cccatagtag gagtacctct tttttgaagc tgaccttcca aaccaagatc
     6601 gcgtatttct tctccaattt gtcttttctt tagatattgg ataattgttc caccgaagat
     6661 gagtgtaata atcaaggaaa gaataaccgc cgacgctgcc ctgaatgata tatactggaa
     6721 aaggccggct ccggggaaat cgaatgcaag atcgaaatat gtgaagagat aatatagcat
     6781 ttatcgtggt tttattagat ggaggtattg tgaaagaatt tctttatcat cgaaaggata
     6841 tttcacgcca ttgatttcct gatatttttc gtgtccttta cctgcaacga ggattacatc
     6901 gccagttttg gctatcatgc aggctatatg gatggcttca cggcggtcgg ggatgctaag
     6961 aactctgtag gaaagtgagg ggttcaaacc agccttcata tcctcgataa tctgcaaagg
     7021 atcttcgctg cggggattat cggaggtaag tatcaaatgg tcggcatttt cggcggctat
     7081 ggctgccatc ttcgggcgtt ttgacttatc gcggtctcct ccggcgccgc acacaacgat
     7141 caagcgacca gcacctgcat gtatatcacg cacggtttcg attacattct gtaaagcatc
     7201 gggtgtgtgt gcataatcaa cgataacatt tatgccttct tcacttttca aaatgtccaa
     7261 tcggccttcg gcgggatgca aattgctcat cacccgtaaa acctcttcag aggtttctcc
     7321 cagtaaaata gctgcaccat aagttgccgt cagattatag gcattgaaac gacccgttaa
     7381 tctgaaccaa gtttcatatc catcgatatg gagcagcatg ctttccaaat ccatttcgag
     7441 tatgcggctg tggaaatcgg ctaaattttt tagaccgtag aaatacttcc gagctttcgt
     7501 attttgcaac ataacttctc cattcggatc atcggcatta ctcaatgcaa aagccgtgtt
     7561 gcccaaattg tcgaaaaaca tctttttggc attcagatat tccgaaaagg tcttgtgata
     7621 atccagatga tcgtgtgtga gattggtaaa aatgcctcca gtatattctt gtccgtaaat
     7681 acgtccctga gctatggcat gcgaacttac ttccatgaat acatggctgc aaccggcttt
     7741 tgccatactg ttcaataaag cgttcaatga aacggcgttg ggtgtagtga gatgggcagg
     7801 tagaatttca ttgtcgatcc tgttttctat ggtcgagata agcccactag tatatccgag
     7861 gctcttgaac aagcgatata acagagtggt tattgtggtt ttaccattag ttccggttac
     7921 acctacaagt ttgagccttc tggaaggata atcgaaaaat gctgaagcta ttggcccaac
     7981 tgccaatgaa gtatttttaa cttgaagata ggttactcga gggtctaaaa cgataggcaa
     8041 agattgacaa acaatgaccg ttgctccgtt ttctatggcc ttttcgataa attcatgccc
     8101 gtctgttttt tgtcctttaa ttgctacaaa aacacttcct tcattggctt ctcgcgaatc
     8161 taatactacc cggtttacag gctggtctaa aactccaata atggattctg cacttatgtt
     8221 aacaataata tcttttagtg ttttattcat cttaagagtg tgataaggtg agggtgatta
     8281 cacttcctgt ctttacttgg gtgcctgcgg gtaatgattg tgcattaacc ttgcctttgc
     8341 cttcgagaat tacttttagt cccatagatt cgagtagata aacagcatct tttgctggca
     8401 tccctttcaa attgggtatg gatttatctg aaaaagataa aatcttagtt gaaacacgat
     8461 gattatggcg ctcgcaatag ccaaattcat tggttagttc taaagtgtcg cagttgaaac
     8521 ccagtaaacg atttacctgt agcaggtcgg gcagccatcc tatcatcgaa acgtgttgct
     8581 gagatatact atcattttta gtgacaaact ccgaggtgta agccgtagat gcatcctgaa
     8641 cgcctaattg agtggcatag atttgatcgg caatggcccg gaaaaccggc gcagccacgg
     8701 ctcctccata gatttttcct tttgaaggat tgcttacaat cactatcagt gaatattttg
     8761 gattatcggc cggaaaatat cctacgaaag aggcattgta attttccttg ttgtattttt
     8821 tgctggttac aatctgtgcg gttcccgttt ttccggcaac tttgtaagga gcaccattaa
     8881 aagctcctct ggctgttcct ttttctgtaa ctgctttcat catttcgcgt gccttgaggg
     8941 cagttttttg agaacagact tcttttatca aaacttgagg ttcatattgt tttaccgtaa
     9001 ttcctgattc ctgtatttgt tttacaaaac gtggcttcat cataatacca ttattggcta
     9061 tggcattata gaaggtaagg atctgcaagg gagtaatctt caattcataa ccaatggcca
     9121 tatagggaag cgaaagtttc gaccaattcg ggtcgtcggc ttttctgatg aagggttcac
     9181 cttcgccggg gatttctata ccaaggggct tccctaaacc catattataa agcgcattgc
     9241 tgaagcgttt tggttttttc ccaaaagcct tgtacacgat tttcgaaact cctacattgg
     9301 atgaatgagc gaatgcatct tcaagagtga tataggttct gttcaaggga tggtcatctt
     9361 tcattacagc gtttctcgac caactaatac ttccttgata cgacactgaa tccttgatat
     9421 tgtatcctgc atattccata accgctacca gcgaaggcaa cttgaagata gaaccgggtt
     9481 cgatgctttc tgctatggca taattgtatg tttcgtcata actgccatcc ttgatctttc
     9541 gaaggttcgc tatgactttg atgtcgccgg ttgcaacttc cattagcacc acgcaacctt
     9601 gataggcatc attttcggtt aattgctcaa gcagagcttg ttcggcaaca tcctgaagct
     9661 tgatgtcgat ggtagttacg atatctttgc catttcgtgg ttctatacgt tgcgcaccca
     9721 ttacaggaat ccattcccct cccgaaatct tttgcata
//
LOCUS       DAOORU010000092         9747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8664_length_9747_cov_5.591519, whole genome shotgun sequence.
ACCESSION   DAOORU010000092 DAOORU010000000
VERSION     DAOORU010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9747
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8664_length_9747_cov_5.591519"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..1698
                     /locus_tag="PLF20_06775"
     CDS             316..1698
                     /locus_tag="PLF20_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOX80740.1"
                     /translation="MSKSNRRDFIKITMGGLAAAGLLKHSGGLRSKPILMQVPERKGS
                     TVMGMTSKALPTVRIGVIGLGMRGSEAVTRLLQVEGTEIVALCDLLPERVEMAKEVVR
                     KQRGTEPVGFFSGEEEWEKLCRRDDIDLVYICTPWEWHTPMAVDAMRNGKHAAVEVPA
                     ALTIEECWELVDTSEATRQHCIMLENCCYDFFELNTLVMARRGLFGEIMHAEGAYIHD
                     LRWLKFDEQNGYYPMWRLEYSKFHTGNPYPTHGLGPIAQIMGINQGDCMEYLTSMSTN
                     QVGMSLYAKETFGPDSKFAKPEYRLGDMNTTLIRTINGKTMMIQHNTTSPRPYSRIHL
                     ISGTKGIAQKYPEERLAFEPEAHRWLNDKEMKKVMEDNQHPLIKQIGEKARKVGGHGG
                     MDFIMDYRLIYCLRNGLSLDETVYDAATWSSLVELSEISVKNNGQPVAIPDFTRGEWE
                     HTPPLGIVTL"
     gene            complement(1752..2597)
                     /locus_tag="PLF20_06780"
     CDS             complement(1752..2597)
                     /locus_tag="PLF20_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOX80741.1"
                     /translation="MQKNTAMPILIIVDFSDNSLEAAIQAANLAELLHEDLLLVHAVN
                     HYTRAYLHENNLDSEWIEESMEWLSQKLTRGREIDVEFVIREGSVYEVISKISRESNA
                     LLIAFSLKGKPGLQKFTPEKIMRMVAASPIPSIIFPRHVCNKFSPMLFPVNLFRGWAK
                     KAKALTHLSQWFGKQVIVVENHSSVMFASEIERERYRFSEKLNSFGLECTFASYLLQG
                     SYTNQLAYYAKRYKAGLLVLMNDEEEESLHFRPGLSETKIIRSKIGLPVLCVSPYMFS
                     QDESR"
     gene            complement(2894..3475)
                     /locus_tag="PLF20_06785"
     CDS             complement(2894..3475)
                     /locus_tag="PLF20_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008146779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOX80742.1"
                     /translation="MEAKSIKGTRTEQNLLKAFAGESQAKNRYIFFAKKAREEGYEQI
                     AAIFELTAENEETHAKQFFRFLEGGMVEITASYPAGVIGTTMENLEQAATGEYEEWSQ
                     LYPEFARIAEEEGFKQIAVKFRTVAKVEIEHEKRYRKLLANIENGEVFKKKQKVRWIC
                     RKCGFVYVGESAPQRCPGCDHPQAFFELLTENY"
     gene            3905..6628
                     /gene="ppdK"
                     /locus_tag="PLF20_06790"
                     /pseudo
     CDS             3905..6628
                     /gene="ppdK"
                     /locus_tag="PLF20_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009126652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
     gene            complement(7306..7394)
                     /locus_tag="PLF20_06795"
     tRNA            complement(7306..7394)
                     /locus_tag="PLF20_06795"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7358..7360),aa:Ser,seq:cga)
     gene            complement(7436..7524)
                     /locus_tag="PLF20_06800"
     tRNA            complement(7436..7524)
                     /locus_tag="PLF20_06800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7488..7490),aa:Ser,seq:tga)
     gene            7659..9140
                     /locus_tag="PLF20_06805"
     CDS             7659..9140
                     /locus_tag="PLF20_06805"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOX80743.1"
                     /translation="MKKFLYIVLLLITVSYSTFAKKLQASLNYATFYSPVDGPYIETY
                     FSVNGNSVVFAWNENGLFRATIEIAMIFRQDEKIVNFKKYELLSPEIRDTTSVAVDFI
                     DQQRIPLPVGKYDFELSITDKNNRKESYVHHIPIEIYYSDTSLVISGIQLVESYNKSE
                     KESILNKNGYELVPKVSTFFSSDQNKLIFYAEIYNAVEIFGEGQKFLVRTYISNYESG
                     FHFNQFSKVKRMDAENVNVVFNEFNITRLPSGNFNLVIEARDRQNRLVSINTLFFQRS
                     NPGYIPDLTDIDSLSLRKSFASHIKNPDTLREYIKCLRPIAADYEKFFIDKGMKDAPV
                     EVMQSYLYSFWKNRSETDPEGAFKAYLEEVAKVQTLFGTSYLRGYNTDRGRIFLQYGP
                     PDVINDYPFDAGLISTLSDNEINKDVGGSVPYQIWHYYQIKDQRNKFFVFYNPHLIPN
                     NYQLLHSDVKGEIYNPVWQSELHRIPFINIDAGNPSSNPNPNQ"
     gene            9174..>9747
                     /locus_tag="PLF20_06810"
     CDS             9174..>9747
                     /locus_tag="PLF20_06810"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOX80744.1"
                     /translation="MPARIAIAILNWNGKEFLVKFLPSLVQFGKLGVEIYVIDNASSD
                     GSVDFLRENFKEVNIIENSENYGFAGGYNHGLKYIDAEYYIILNSDIQLTEGWIDPMV
                     EYLDGHAEVAACQPKIRSYDEPERFEYAGASGGYIDFWGYPFCRGRLFQAIEKDYGQY
                     DDITEIFWASGACLFIRKDCFWEVGGFDEDF"
BASE COUNT         2985 a         1869 c         2073 g         2820 t
ORIGIN      
        1 tagcaaactc tccgaattga accccgtgaa gaaactttga gttcgaagag gaaatgaata
       61 tttttaatgc gaaagcctta tctcttcagc cagattgaac tttgctctga atattcataa
      121 tatatttata attgctcatt ataagccatt tttgaaaaaa gaattatgag atttatagaa
      181 aaaatttagc atgaattggc tgaattggat agtttgagct gtttgtcaga gaacaataag
      241 ataggtggag aaagaattcc gaccgaaata aggaaaaaat tttgaaatta gcgccaataa
      301 attatcctaa tcattatgag taagagcaat cgcagagatt ttatcaagat taccatggga
      361 ggacttgctg ctgcagggct tcttaagcat tcagggggtt taaggtcaaa acccattctt
      421 atgcaggttc cagaaaggaa gggaagcacg gtaatgggta tgacgagtaa ggccttacca
      481 actgtaagaa tcggtgtgat tggattaggt atgcgcggca gtgaagctgt tacgagatta
      541 ttgcaagtgg aaggaaccga aatcgttgca ttatgcgatt tattgcccga aagagtagaa
      601 atggccaaag aagtggtaag aaaacaaaga ggcactgaac ctgtgggctt tttttcgggg
      661 gaagaagaat gggaaaaact ttgccgccgc gacgatattg atctggttta tatttgtact
      721 ccgtgggaat ggcatacccc aatggccgtc gatgccatgc gaaacggaaa acatgccgct
      781 gtggaagtgc cggcagcctt aaccatagaa gaatgctggg aattggttga tacttccgaa
      841 gcaacacgtc agcattgcat aatgctcgaa aactgctgtt acgatttttt cgaactcaat
      901 acccttgtaa tggcccgtag aggactattt ggagagatta tgcacgccga gggagcctat
      961 attcacgatc taagatggtt gaaattcgat gaacaaaatg gatactatcc aatgtggcgt
     1021 ctcgaatata gtaaatttca tacaggaaat ccctatccta cccatggact cgggcctata
     1081 gcccaaatca tgggaatcaa tcagggcgac tgcatggaat atctcaccag catgtcgact
     1141 aatcaggtgg ggatgagttt gtatgcaaaa gaaacttttg gccctgatag taagtttgcc
     1201 aaaccggaat acaggcttgg cgatatgaat acgactctga ttcgcacaat caatggcaaa
     1261 acaatgatga ttcagcataa tacgactagt cccaggcctt acagtcgtat tcatcttatt
     1321 tcggggacaa aaggaatagc gcaaaagtat cccgaagaac ggctggcttt cgagcccgag
     1381 gcgcatcgct ggctcaatga taaggaaatg aaaaaagtaa tggaagacaa tcaacatccc
     1441 ttaatcaaac agataggaga gaaagccaga aaagttggtg gccatggcgg tatggatttt
     1501 atcatggatt accgtttgat ttattgcctg cgtaatggcc tatctctcga tgaaaccgta
     1561 tatgatgctg cgacatggtc ttctttggtc gaactttccg aaatatcagt gaaaaataat
     1621 ggtcagcccg ttgctattcc tgattttacg cgaggagaat gggaacatac acctccactg
     1681 ggaatcgtta cgctttgatt tttattgaaa tcgtttttct tcggtgatgg tttgttcccg
     1741 atttattgtc atcaccttga ctcatcttga gaaaacatat atggtgaaac acaaagtaca
     1801 ggtaaaccga tcttactgcg gatgattttt gtctcggaaa ggccaggcct gaaatgtaat
     1861 gattcctctt cttcatcatt catcaataca agcaatcccg ccttgtaacg tttcgcataa
     1921 tatgccaatt gattcgtata gctaccttga agtagataag aagcaaaagt acattcgagc
     1981 ccgaaagagt tcagtttttc actaaaacgg tatctttcac gttcgatctc actggcgaac
     2041 ataacgcttg agtgattttc tacaacgatt acctgcttcc caaaccattg actcaaatgt
     2101 gttaatgctt tggctttttt agcccatcca cggaatagat tcacaggaaa aagcatgggg
     2161 gaaaatttat tgcatacatg ccttgggaaa ataatggatg gtataggtga agcagcaacc
     2221 atgcgcataa ttttttcggg tgtaaatttt tgcaaacctg gtttcccttt caagctaaaa
     2281 gcaattaata aggcattact ttctctggaa attttcgaaa tcacttcgta aacacttcct
     2341 tcacgaatta caaattcgac atcgatttct cttcctcgtg ttagcttctg agaaagccac
     2401 tccatcgatt cttctatcca ttcactgtcg agattgtttt cgtgcagata tgcccgcgtg
     2461 taatggttta ctgcgtgtac caaaagcaaa tcctcgtgca gcaattctgc taaatttgct
     2521 gcctgaatag cggcctctaa gctattatcg gaaaaatcaa caataattag gatgggcatg
     2581 gccgtatttt tttgcaagta acaaaaattt tttctattga agttatttct cagaaaatat
     2641 atttgctaat tatttcgagt cctgtattat cttatggttg aatgaattaa aaataagagg
     2701 catgcatagc atggcatctt tgatatcgga aatataataa tttatccttt agagtgggat
     2761 ttctctccta tcgtgaaaat ttgaaaaacc cttcgaaaac gcaataagat gaaccttaac
     2821 aagtgataca ttttttaatt tcattcctta ataccaaggg ttcatcggat tttgtcatta
     2881 ttcgaggaaa ccttcaataa ttctcggtca ataattcgaa aaatgcctga ggatgatcgc
     2941 atccggggca acgctgagga gcactttctc ctacgtaaac gaagccgcac ttacgacaaa
     3001 tccagcggac cttttgcttt ttcttgaaaa cttctccgtt ttcaatattg gctaataact
     3061 tacggtagcg tttttcatgt tcgatttcta ctttagcaac cgtacgaaat ttcacggcaa
     3121 tctgcttgaa cccttcttcc tcggcaatgc gcgcaaattc agggtataat tgcgaccatt
     3181 cttcgtattc gccggtggct gcttgttcga ggttttccat agtcgtgccg attacacctg
     3241 ctggataaga ggcagtaatc tcaaccattc ctccttcaag aaatctgaaa aactgcttgg
     3301 catgcgtttc ctcattctcg gctgtgagct cgaaaatggc tgcaatttgt tcataccctt
     3361 cttcccttgc cttttttgcg aagaaaatat agcgattctt tgcctgcgat tctcccgcaa
     3421 aggcctttaa cagattttgt tcggttcgtg tcccttttat actttttgct tccataacaa
     3481 aatgattatt aagatgaaga ttgttttatt tttcatttaa gattcaagtt cgagtcatcg
     3541 aaatgtttca acatagctac aaagatatac ttttttatat gtatatggaa aaatgataat
     3601 cagaaaccca aattgaagtc tttcattgtt agtttttatt cgtgatgaat gataatacca
     3661 tttccctttc attcagaata tcaagagaaa gtttagtgaa atgggaatcc atgactttga
     3721 tttttctggt gaagcatata ttttcgaaga tttataaaat gaggtcagtg gtgagatgta
     3781 ataagaaatc tttaagttat tcagaatatt ttacgaaaag ttatactttt gcaggcttgt
     3841 ttgaaaaata tttcatgaaa ttgaaaatca tttgaataat caacccaaat aattgaaact
     3901 gaaaatggca aagaaactca aaaaacacgt ttacctattc ggcaacaaga cagccgaagg
     3961 aagtgccgaa atgaaagatt tactcggtgg caaaggagcc aaccttgcag agatgaattt
     4021 gataggaatg cctgtgccac ctggctttac catcacgacc gaggtttgtg atgcctatta
     4081 taaattgggc cgcgaagctg ttattgaaat gataaaggat gaggtagaag ccggaatccg
     4141 ctttatagaa aatattatgg gcactcggtt tggagatcct aacaatccgt gtttggtatc
     4201 tgttcgttcc ggcgccaaag cctctatgcc cggcatgatg gataccattc tcaaccttgg
     4261 tttgaatgtt gccgtagtgg aaggtatgat caaaaaaacc gggaatccac gatttgtatg
     4321 ggatagttat agacgtttcg tacagatgta tggagacgtc gttttgggca tgaaacccgc
     4381 ttcgaaacaa gatgtggatc ctttcgagga aattatcgaa gaattaaagg aagaacgcgg
     4441 cctacatttt gacaatgaat tgactaccga agatttgaaa gaattagtgt ttcgatttaa
     4501 aaaggctgta agagaacgta cagggaaaga ttttcccgaa aatccatggg atcagctctg
     4561 gggtgccatc atggcagttt ttgattcgtg gatgaacgat cgtgctatct tgtatcgtca
     4621 gttgaacaat atccctgatg attggggaac ggcagttact gtacaggcaa tggtatttgg
     4681 caacatgggc gaaacttctg ctacaggagt ggcttttacg cgtgatgctg ccacagggga
     4741 aaaaatattc aatggggaat atcttatcaa tgctcaaggt gaggatgtag tagccggaat
     4801 ccgtacccct caacaaattt ctctcgaagg ttctagaaga tgggctgcct tagctggcat
     4861 ttcggaagaa gaacgacgct ctaaataccc ttcgctcgaa gaaaccatgc ctcatattta
     4921 tcaggaactt ctcgaaatac agcaaaaact cgaacagcat tatcgcgata tgcaagatat
     4981 cgagtttaca attcaggaag ggcgtttgtg gatgcttcag acccgtaacg gtaagcgtac
     5041 gggcactgct caggtaaaga ttgccataga tatgctccat gaaggaatga tagacgaaaa
     5101 aaccgctctc ttgagagttg aacccaataa acttgacgaa ctactacatc cggtattcga
     5161 caatgaagct attaagaaag cagaagtcat tgccaaaggt ttacctgctt caccgggagc
     5221 agcgacagga cgtattgtat ttcaggccga tgaagctgag cactgggcag ctagaggcga
     5281 gaaggtgatt ttagtgcgta tagagacttc gcccgaggat ttgcgtggaa tgaatgcggc
     5341 cgagggaata ctcacttctc gtggaggcat gacttctcat gctgccgtag ttgcacgcgg
     5401 aatgggcaaa tgttgtgtat cgggagcagg ttctcttaaa attgattata aacaaaaaac
     5461 tatccaaacc aatggaatga cgttcaagga aggcgactgg atttcattga atggaagcac
     5521 aggtgaagtt tataaaggaa aaattgctac ccgtgatcct gaactcagtg gtgactttgg
     5581 tgaactaatg cgtttgactg ataaatattc acgcatgtac gtcagaacca atgctgatac
     5641 tccaaacgat gccagagtag cccgtaactt tggtgcgaga ggaattggcc tgtgtcgcac
     5701 cgaacatatg ttttttggag gcgaaaagat caaggccatg cgcgaaatga tcttagccga
     5761 tgacgaagcc ggccgccgga aagccctcga taaacttctt cccatgcaac gcgaagattt
     5821 cgaaggcatc ttcgaagcca tgcacgattt accagttact atccgtttgt tagacccgcc
     5881 tttacatgag ttcgtccctc aagaagaaat caaccagcgc gaaatggcaa aagaaatggg
     5941 acttactctc gaacatatca aggcaaaggt cgaagaactt catgaagtga atcctatgtt
     6001 aggccatcgt ggatgccgcc tgggcaatac ttaccccgaa atctctgaaa ctcaatcccg
     6061 cgcaattatc gaagctgcat tgaaccttaa aaaaagagga ataaaggcaa ggcctgaaat
     6121 catgattccg cttacaggta cacttaagga aatgcaaatc caggaggata ttgttcggtc
     6181 aaccgccgaa aaagtgtttg tagaatataa cgaccgaata gactatctgg taggaaccat
     6241 gatagaagtt ccacgtgcta ccctcgttgc cgatcagatt gcccaatcag ccgaattttt
     6301 ctcgtttgga accaacgacc taacccagat gaccttcggc tattcacgtg atgacgctgg
     6361 aaaattcctt ccggtttatc taaaaaaagg aatcctgaaa aatgatcctt ttcaagtcct
     6421 cgaccaagag ggagtaggac aacttattcg tatgggcgtt caaaaaggac gtcaaacacg
     6481 tgaaaaattg aaaatcggaa tatgtggcga acatggtgga gaaccctcct caatcgaatt
     6541 ttgcaattca gttgatttcg attatgtaag ctgctcgccc taccgtgtac ctattgcccg
     6601 ccttgctgct gctcaggctg tcctgaaaca atcccccaaa cccgaatcag aagaagaaac
     6661 atataagaag gataaaaagg gcaagaagga taaaaagggc aagaaggata aaaaggataa
     6721 aaaggacaaa aaagataaaa agaacaaaaa gtcgaaaaag gaaaagaaga acaagaagaa
     6781 gtgatccgat gatctgaatt ttatattgta gagcaggatg cataagggaa taattctata
     6841 tacatcctgc ttttttatag aaaatgtatg ggactgcagg tgatattaac ataagtgttg
     6901 cgagatacaa cagaaaacaa gagacaaaca gaaaaggaga aattcatttt atactttaac
     6961 tttttgctac cagttgttat cccttgggtc actttgcaat ttcaacccta aaaagtggta
     7021 agagtccaca taatagtgca gattcttata agacttacaa taattccaac cttgcactaa
     7081 ggttttttta ctccgtaatc tacacctcgc acccagtttc ctgtcgtttc acacccaatc
     7141 gctaagcctg caaagtctct atgtattata ttatatgttt tcatgcactc tgtgacccgc
     7201 aacaccaatc ccttattaca aactgatgga aaaatacggt aaattttaat tggaatagtt
     7261 attcgagaaa tagggtggtg ggaaatatag aagttgaaaa aattgcggag agggggggat
     7321 tcgaaccccc ggtccctctt acgtaggaca acggttttcg agaccgccgc attcgaccgc
     7381 tctgccacct ctccctttta taacaatatt agttagatgc atacataaaa ttcagcggag
     7441 agagagggat tcgaaccccc ggtccacctt ccggcgaaca acggttttca agaccgccgc
     7501 aatagaccac tctgccatct ctccagcggc aaaagtacaa atttttcttc tcccatctca
     7561 atttttattt ttacgtttac tcgaatggtt tggcactaaa aatgtgtaat tttgtataaa
     7621 atagtatttt gttctatttt tcctaaactg tcctcgaaat gaagaagttt ttatacatag
     7681 tgttgctctt gattacggtt tcttattcca cttttgcaaa aaaactacag gctagcctca
     7741 attatgctac cttctattct ccagtggatg gcccttatat tgaaacctat ttttcagtca
     7801 atggaaattc tgtagttttt gcttggaatg aaaacggatt gtttcgggct actatcgaaa
     7861 tagctatgat tttcagacaa gatgaaaaaa tagttaattt caagaaatat gaactactta
     7921 gccctgaaat acgcgatacc acttcagtag cagttgactt tatcgatcag cagcgcatac
     7981 cccttcccgt gggtaagtac gattttgaac tgagcattac cgataagaat aatagaaaag
     8041 agtcttatgt tcatcatatt cctatagaaa tttattacag cgataccagc ttggttattt
     8101 ctggaatcca gttagttgaa tcatacaata aaagtgaaaa agagtcgatt cttaataaga
     8161 atggatatga gcttgttcct aaggtaagta catttttctc ttccgatcag aacaaactga
     8221 ttttttatgc cgagatttac aatgcggtgg aaatttttgg tgaaggccag aaatttctcg
     8281 tgcgaactta tatctccaat tatgagtcgg gatttcattt caatcagttt agtaaggtaa
     8341 agagaatgga tgccgaaaat gtcaatgtag tgttcaatga atttaatatt actcgtttgc
     8401 ccagcggaaa tttcaactta gtgatcgaag cccgagatcg gcaaaatcgt ttggtcagta
     8461 ttaacacctt attctttcaa aggagcaatc cgggttatat tcccgactta acagatattg
     8521 acagtttatc gttgcgtaaa tcctttgcct cgcatattaa gaaccctgat accctacgag
     8581 aatatattaa atgcctgcgc cccattgctg ccgattatga aaaatttttc atcgacaagg
     8641 gaatgaaaga tgcacccgtg gaagtgatgc agtcttattt gtattctttc tggaagaatc
     8701 gttcggaaac cgatcctgaa ggtgcattta aggcctatct ggaagaagta gccaaggtcc
     8761 agactttatt tggcacttct tatctaagag gatataatac cgatcgtggt cgtattttct
     8821 tacagtatgg cccgcccgat gtgattaacg attatccctt cgatgcagga ttaatatcta
     8881 cgctatcgga taatgaaatc aataaagacg ttggcgggag cgttccttac cagatatggc
     8941 attattacca aattaaggat cagcgcaata aattttttgt attttataac cctcatttga
     9001 ttcctaataa ttatcaactc ttgcacagtg atgtcaaggg tgagatctat aatcctgtat
     9061 ggcaaagtga gttacataga attccgttta taaatatcga tgcaggcaat cctagttcaa
     9121 atccaaaccc aaaccagtaa tcgcatgaga attttatctt taatataaac cttatgcctg
     9181 cgaggattgc tattgctata ctcaactgga atgggaagga atttcttgtc aaattcttgc
     9241 cttcccttgt tcagtttggt aagcttggtg ttgaaattta cgtgatcgat aatgcttctt
     9301 ctgacggttc ggttgatttt ttaagagaaa atttcaaaga agtaaatatc atcgaaaatt
     9361 ccgagaacta tggatttgcc ggaggttaca atcacggact caaatacata gacgcagaat
     9421 attatatcat tttgaattcc gatattcagc ttaccgaagg ttggattgat ccaatggttg
     9481 aatatttaga tggccatgcc gaggttgctg catgccagcc taagattcga tcttatgacg
     9541 aacctgagag atttgaatat gccggtgcct cgggtggcta tatcgatttt tggggttatc
     9601 cgttttgtcg tggccgtttg tttcaggcta tcgagaaaga ttatggccaa tacgacgata
     9661 ttacagaaat attttgggct agtggggctt gtttgtttat cagaaaagat tgtttttggg
     9721 aagttggtgg gtttgatgaa gattttt
//
LOCUS       DAOORU010000093         9654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8765_length_9654_cov_5.512866, whole genome shotgun sequence.
ACCESSION   DAOORU010000093 DAOORU010000000
VERSION     DAOORU010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9654
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8765_length_9654_cov_5.512866"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="PLF20_06815"
     CDS             complement(<1..571)
                     /locus_tag="PLF20_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80745.1"
                     /translation="MRKLYITFILLIAGIGLNAQNAAKLRSNLPQAKAPERPMIAIEP
                     VQQNPISAATILPGEYKKGQKGIVSVIPIGTSANAFTYGYGGGQKTMVFADDNLNAVI
                     NIHRMGPGTTPPGLSGYLAMDRGINGAAKPGDWSSNVEIYNSTISGGTYFLDAARYPQ
                     GVIYNPEGNTDPANAYAIFFAPNLSSTATT"
     assembly_gap    825..924
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1244..3103)
                     /locus_tag="PLF20_06820"
     CDS             complement(1244..3103)
                     /locus_tag="PLF20_06820"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80746.1"
                     /translation="MRKFTFILVGMLVVSGLMAQTTRVKVNGCNIAVKMEKATSEGIS
                     PVNVVSPYVNPVNDAIEEDIGTTIYDLPSNSCTPYGRLYVYPDGTKAGVWTIGKNPTA
                     YPDRGTGYNYYNGSSWGPDPTLRIESVRAGWPSYAPCGPNGEIVVSHQSGTLPLVVSK
                     RETKGSGNWEQSFLNPPSGATGMLWPRMITTGQDHNTVHIIALTAPTGNNGTVFQNMD
                     GALVYCKSEDGGTTFGEWQLLDGLTSEFYLGFGGDSYSWLIPYENDIAFVICDNITDL
                     IIMHSTDNGETWEKIIVWEHPYPFYSSSMATDKFYCPDGSAHGVIDKYGKIHLAFGVN
                     RSYSDGSSSYWFPWVDGLAYWNSDIPVWLGGDTIALHPDTLYEHGRLIGWMQDVNGNG
                     SLDLIGLTSGSYPLYYLSPTSMPQIIYDNDNDIIYAVFVSITEGYHTDSQDYRHLWVR
                     VSYDKGLTWSDFTDLVANIFHIYDECVFPVISPTLDGGCPAIIYQRDEEPGLSARGDE
                     DPPTTNYITYMKINEYVGVKEVAKHTFTVEQNYPNPSEGMTKVIVEVGQPNRLILSVS
                     TVTGQKVMVIDKGNVNAGQHIFEINTSNLQSGIYFYTVRCGNESMTRKMVVNN"
     gene            complement(3282..4430)
                     /locus_tag="PLF20_06825"
     CDS             complement(3282..4430)
                     /locus_tag="PLF20_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80747.1"
                     /translation="MELIRVSDKRTSRMFLDTARIIYRNDPVWVCPLDQTVEDIFDPR
                     QNVFFSHGEAERWVLKEKGTLIGRVAAFINYKKAYKGEYPIGGMGFFECIDDEKAADM
                     LFNSCQHWLEQKGMKAMDGPINFGENDSFWGLLVEGFTHPGFGMNYNPPYYRRLFENY
                     GFKRYYSQITKHLDIHAPFPERFWKIAHWILQKPEFVFRHLQVSKIQEYAGDFKKIYD
                     QAWQWHENFAPIEIETLIRTFEESRSIIDPELIWFTYYRGEPCGFFVVFPDLNQIIKP
                     FYGKLNLLNKLRLLYRVRTHKMNRARMLIFGIIPKVQRYGVEAGLIWHLNEAFKKKPW
                     YNELELSWVGDFNPKMQSLLKAVGGVKAKEHWTMRKFFDENLSFAPIT"
     gene            4648..6078
                     /locus_tag="PLF20_06830"
     CDS             4648..6078
                     /locus_tag="PLF20_06830"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOX80748.1"
                     /translation="MKKIHILVVKSYLGPLVLTFFIALFVLLMQFLWKYVDDLVGKGL
                     EWFHIANLMVYASTTFVPLALPLAILLSSIMTFGNLGEHYELVAMKSAGISLGKAMRP
                     LIVLSVLISIGAFLFSNMVLPVANLKFKSLLYDIRKQKLALNIQPGLFYNGIDNYTIR
                     VDQKNDETNTVYGVIIYDHSAQLGNMKFTTASEGKMQLTPDEKFLIFTLHNGYNYEEL
                     FNEKDYRNRRQFQIVRFDENRQKFDMTGFGLQRTDEELFRKNYQMMNVSQLNYAIDSL
                     EKEYRIRVKEVSRSIYSRYSILQNADTLHKETSMRFSLGQLDASERERVITIALQNTR
                     NLKEYLSVTENDQRIRNTLINRHKVVWHQKFTLSIACLLFFFIGAPLGAIVRKGGLGL
                     PVVMSAFIFIIFHIISITGEKYAITGSAMVAPAVWTSTLVMFPLGIWLTWKAISDSPI
                     LDMDTYNRFFNHFIPKKWKLPLSEQV"
     gene            6120..7325
                     /locus_tag="PLF20_06835"
     CDS             6120..7325
                     /locus_tag="PLF20_06835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOX80749.1"
                     /translation="MDILFLCNKPPFPAREGGPIAMNMLIEGLIEQGHQVKVLTVSSY
                     KYPFRPELIPPDYMEATCIEAVEVDLHIRPFKAFINLFARKSYHVERFISKTFRRRLI
                     NILQEHEFDIVQLETVFLCPYLPDIRNHSMARIVLRTHNIEHLIWKRLWENSKPGPKK
                     VYLKHLWTTLRKYELGILDKADGIVAITRKDAEFLKPFTRVPIIDISFGIDSSQFPEP
                     TYDDCEIPSLFHIGSMDWIPNQEGIQWFLSEVWPKVYDNFPFLKFYLAGRNMPEWLLN
                     GFWPNVVVLGEVEDARKFMLSKWIMVVPLLAGSGIRVKIIEAMANGRPVIASPIGAEG
                     IEYTEGENILLASTPDQFLEAIRKCIDNPQFAIQLGRNAHLLVMKEHDRRKLITELTH
                     FYHRLVYTP"
     gene            7402..8571
                     /locus_tag="PLF20_06840"
     CDS             7402..8571
                     /locus_tag="PLF20_06840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX80750.1"
                     /translation="MKLLVILSRVPYPLEKGDKLRAYHQIRCLSKYHQITLIALTDQK
                     VHPQAKDKLLNFCDEVYFLKLNKLGILLNVIGAFFRGWPLQIGYFYNKKVKKEIDSIV
                     RQVKPNHIYCQLIRTAEYIHDFDIPKTIDFQDTFSIGMKRRYEKANFLLKPFFHLEYK
                     RLMRYERRIFDLFHNHTLISCPDKELFPHPNKYKIFIIENGVDFEYFETKSPIKKYEV
                     IFTGNMGYPPNIDAAFYLANEIMPLVRKQFPQAHLVLAGATPHPKIKRLANTYTIVTG
                     WVDDIREWYDTSMIFIAPMRLGTGLQNKLLEAMAMHLPCITSSLANEALHAIPNEEIL
                     IGDSAEEYANHIIDLLVNPALYNKLILNGHRFVIENYSWENASEKLNAILTHQKD"
     gene            complement(8701..9246)
                     /locus_tag="PLF20_06845"
     CDS             complement(8701..9246)
                     /locus_tag="PLF20_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HOX80751.1"
                     /translation="MKSAKLRLVLFVVLLTTTIAFQPEEKSTVQWQTFESAIAKNEGK
                     IKKKVFIDVYTDWCGWCKKMDATTFSEKAVASYMNEHFYCGKLNAERCDSIRYNGKIY
                     VNTCPSSKKSAHQLAIALLQGRMYYPSYVILDENNQKLTSFEGYLKPEEMMPILVYFA
                     ENHYLKMSWENFKKEYDDHKK"
     gene            9371..>9654
                     /locus_tag="PLF20_06850"
     CDS             9371..>9654
                     /locus_tag="PLF20_06850"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80752.1"
                     /translation="MPELTYEFVKSLPKAELHCHLDGSLRMGTILELAKEQNIELPAW
                     DEENMKKILVPPLTCESLEEYLKPFDIPVSVLQTREALERATYELAQDCA"
BASE COUNT         2922 a         2060 c         1750 g         2822 t
ORIGIN      
        1 aagtggtagc agtactggaa aggttaggag caaagaatat agcataggca ttggctggat
       61 cggtatttcc ttcagggtta tagataacgc cttgcgggta acgggcagca tccaagaaat
      121 atgtacctcc agaaatagtg gagttataga tttcaacatt gctcgaccag tcacctggct
      181 tggcagctcc attaataccc ctgtccatgg caagatagcc agaaagaccc ggcggagtag
      241 ttccaggacc cattctatga atattgatca cggcattcag attgtcgtcg gcaaaaacca
      301 tggttttctg gcctccacca tacccataag taaatgcgtt ggccgaagta ccaataggaa
      361 tgactgatac aatccctttc tgaccttttt tgtattcgcc cggcaaaata gtggcagcag
      421 aaatgggatt ttgttgaaca ggttcgatag caatcattgg tcgttccgga gctttagcct
      481 gtggtaggtt ggaacgcaat tttgcagcat tctgggcatt caacccaata cctgcaatca
      541 aaagaatgaa tgtaatgtag agctttctca tagcagtaaa ttttatggcg taaagataca
      601 tgattttaat tgaatacggt gcaagaattt cattatctat tctattaaaa atccctttag
      661 aatttggatt cgaatcatta cttcgctgaa gatcaatgga aagaataagc ataatatgaa
      721 taaataatta aaaaattttc ttaatatctt cttaaccctt gttcgacaaa aaaaaaggcc
      781 atcccttcga agaggacggc cttttcccaa gatcgatttt atccnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn nnnnnnnnnn nnnntttaca gggcttatcc cttccgaagt ggctttttcc
      961 attttcacgg ctatgttgcc gccgtttacc tttacacggg tagtctgggc cattaaccca
     1021 ctcactacca gcacacaaac tacaattaaa gtaaattttc tcatggcgtt ggttatttgg
     1081 ttaaaataat gctgtaaaaa taatacaatt tttaatcaaa tcataaaaat atcattcgat
     1141 ttcaaaaaaa ttcttgaatg aagttcaaaa tatgatgaga tgaattaata aaaaaaggcc
     1201 atcccttcga agaggacggc cttttcccaa gatcgatttt atcctaatta ttcacaacca
     1261 tcttcctggt catgctctca ttgccgcaac gtacagtgta aaaataaata cccgactgta
     1321 aattgctcgt gttgatctcg aatatatgct gaccagcatt tacattgccc ttgtcaatca
     1381 ccattacctt ctgaccagta acagtgctta cactcaaaat taatctattc ggctggccaa
     1441 cctctactat caccttcgtc ataccttccg atggattagg ataattctgt tctacggtaa
     1501 aagtgtgctt agcaacctct tttactccta catactcatt gattttcatg taggtaatgt
     1561 aattcgtcgt aggtggatcc tcatcccccc ttgccgataa accaggctct tcatccctct
     1621 gatatataat tgctggacag cctccatcca gcgtcggact gattacagga aatacacact
     1681 catcatagat gtgaaaaata ttggcaacca aatccgtgaa atcactccat gtcaatccct
     1741 tatcatacga aaccctcacc cacaaatgac gataatcctg cgaatcggta tgatatccct
     1801 ccgttatgct cacaaatacg gcataaataa tatcattatc attgtcgtat atgatctgcg
     1861 gcatcgaagt cggacttaaa taatacaggg ggtaactccc gctggtgagg ccaatcaaat
     1921 ctaaactgcc attcccattc acatcttgca tccatcctat taatctccca tgctcataca
     1981 atgtgtcggg atgtaaagca atggtatcgc ctcccaacca taccggtata tccgaattcc
     2041 aataagccaa accatccacc cagggaaacc aataggaact cgacccatca gaataggaac
     2101 gattcactcc aaaagccaaa tgtatcttcc catacttgtc gattactcca tgggcagagc
     2161 catccggaca ataaaactta tccgtagcca ttgaggagga ataaaatggg tagggatgct
     2221 cccatacgat tatcttctcc cacgtttcac cgttgtcggt ggaatgcatg atgattagat
     2281 cagttatatt atcacagatt acaaaagcaa tatcattctc ataaggaatg agccatgagt
     2341 atgaatctcc tccaaatccc aaataaaatt ccgaagtcaa gccatcgaga agctgccatt
     2401 ctccaaaggt agtacctcca tcctccgact tgcaataaac aagtgcgcca tccatatttt
     2461 gaaagacagt accattattg ccagtagggg cagtaagggc gattatgtgg actgtgttat
     2521 gatcctgacc cgtagtaatc attctgggcc ataacatacc tgttgctccc gaggggggat
     2581 ttaaaaatga ctgttcccaa ttgccactgc ctttagtttc acgtttcgaa accaccaagg
     2641 gcaaggttcc actctgatga gaaaccacga tctcgccatt gggcccacaa ggagcatacg
     2701 atggccaccc agctctcaca ctttcgatgc gtagagtagg atcgggtccc catgaagaac
     2761 cattgtaata attatatcca gtgccacggt cgggataagc tgttgggttt ttcccaatcg
     2821 tccatacacc ggccttcgta ccatccggat atacatacaa acggccatag ggtgtgcaag
     2881 aattggaggg aaggtcatat atggtcgtac ctatatcctc ttctattgca tcgttaaccg
     2941 gatttacata aggactcacc acatttacag ggcttatccc ttccgaagtg gctttttcca
     3001 ttttcacggc tatgttgcag ccgtttacct ttacacgggt agtctgggcc attaacccac
     3061 tcactaccag cattccaaca agaatgaatg taaatttcct catagatttt tagtttaaaa
     3121 aggttgataa ttattaaatc accacaaaaa tataattttt ttcaagtgca agattaattc
     3181 agtaaaaaaa atattgaatc atccaaaatt aatgattcat gataaattca atagagaatc
     3241 atgtaaaaac acattcatga tttgcttgaa ctttttaaat atcaggtaat tggagcaaat
     3301 gaaagatttt cgtcgaagaa cttgcgcatg gtccaatgtt cttttgcctt aactccacca
     3361 acggccttga gcaaggattg cattttaggg ttaaagtcgc ctacccaact caattcaagt
     3421 tcgttatacc aaggtttttt cttgaatgcc tcattcaaat gccatatcaa ccctgcttcg
     3481 acaccatagc gctgaacttt gggaatgatg ccgaaaatga gcattctggc tctattcatt
     3541 ttatgagttc tgactcggta aagcaatctt aatttattca acaggtttag tttgccataa
     3601 aatggtttga taatctggtt cagatcgggg aaaaccacaa aaaatccgca aggttcgcca
     3661 cgataataag taaaccagat taattctgga tcgatgatag accgactttc ttcaaaagtt
     3721 cgaatcaaag tttcaatttc gataggagca aaattctcat gccattgcca tgcttggtca
     3781 taaattttct taaaatcgcc tgcatattct tgaattttgc ttacttggag atgtcggaat
     3841 acaaattcgg gcttttgcag aatccaatgg gcaattttcc agaaacgttc aggaaaagga
     3901 gcatgaatat cgagatgttt ggtaatttgg ctataatatc gtttaaaccc ataattttcg
     3961 aacaaacgcc ggtaatacgg agggttataa ttcattccaa agccaggatg cgtaaatcct
     4021 tcgacgagca acccccaaaa cgaatcgttt tcgccaaaat ttatgggacc atccatagcc
     4081 ttcatccctt tttgctcgag ccagtgttga caactattga atagcatgtc agcagccttt
     4141 tcgtcgtcaa tgcattcgaa aaatcccata ccacctatgg gatattcacc cttataagct
     4201 tttttataat tgatgaaggc ggctactcga ccaatcaatg tccctttttc tttcagcacc
     4261 cagcgttcgg cttctccatg cgaaaaaaat acattttgcc ttggatcgaa aatatcttca
     4321 acggtctgat cgagtggaca aacccacaca ggatcattcc tataaattat tctggccgta
     4381 tcaagaaaca tccgtgaggt tcgcttatct gatactcgaa tcaattccat aggagttacg
     4441 atttcggcaa aattaaattt tttactattt atgccttcaa aatcaattca gctgaaaacc
     4501 caacatgata aataattgaa aacccgttta aaatttcaaa tatttttaat gtcagatatt
     4561 tacacatcga tgtgtgaaac gggtttgcga tacgaaatcg ccgaatggag tgtttcttat
     4621 taattttgca cagttaattc aagcgtcttg aaaaagattc acattttagt ggttaaatcg
     4681 tatttagggc cattggtgct tacatttttc atcgcacttt tcgtcctttt gatgcaattt
     4741 ttatggaaat acgtcgacga tctggtaggt aaagggctcg aatggttcca tattgccaat
     4801 ctaatggttt acgcttctac aaccttcgtt cctcttgctt tacctctggc tatcctgctt
     4861 tcttcaatca tgactttcgg aaatcttggc gaacactatg aactcgtggc catgaaatcg
     4921 gccggcatca gtctgggcaa agccatgcgt ccacttatcg ttctatcggt attgataagc
     4981 attggagcct ttttattcag caatatggtt cttccggtgg ccaacctgaa attcaaatct
     5041 ttactgtatg atatccgtaa acaaaagctg gcattgaata tacagccggg cttattctac
     5101 aacggaattg acaattacac catcagggtc gatcaaaaga acgatgaaac caacaccgtt
     5161 tacggcgtaa tcatttacga tcatagtgct cagttgggca acatgaagtt taccacagcc
     5221 agtgaaggta aaatgcagct tacccctgac gaaaaattcc ttatttttac acttcataat
     5281 ggttataact atgaagaatt gttcaacgag aaagattatc gcaatcgcag gcagttccaa
     5341 atcgtacgat tcgatgagaa taggcagaaa ttcgatatga ccgggtttgg gcttcaacga
     5401 acagatgagg aattatttcg taaaaactac cagatgatga atgtgagcca gttgaattat
     5461 gccatagact cactcgagaa agaatacaga atacgggtaa aggaagtctc acgttccatc
     5521 tattcgcgat attccatttt gcaaaatgca gatacattgc acaaagaaac ttccatgaga
     5581 ttttctctgg ggcagctcga tgctagcgaa agggaaaggg ttatcacaat tgctcttcaa
     5641 aacacgagaa acctgaagga atatttatcg gttacggaaa atgatcagag gatacgaaat
     5701 actctgatca accgccataa agtcgtttgg catcaaaaat ttaccctctc tattgcttgt
     5761 ttgttgtttt ttttcatagg agccccgctg ggagccattg taagaaaagg ggggctaggt
     5821 cttcctgtag taatgtctgc tttcattttc atcatcttcc atattatctc tattacggga
     5881 gaaaaatatg ccataaccgg aagtgcaatg gttgccccgg cagtttggac ctctaccctc
     5941 gtcatgtttc cccttggaat atggcttaca tggaaggcta tttccgacag tcctattctc
     6001 gacatggata cttataaccg atttttcaac cattttattc caaaaaaatg gaagctcccc
     6061 ttatcagaac aagtataaac tgagaatcat caacacttca gtatgcgaat agaataaaaa
     6121 tggatatact ctttttatgc aacaagccac cgtttccagc ccgtgaggga ggtcccatag
     6181 caatgaatat gctcatagaa ggactgatcg aacaggggca tcaggtgaaa gttttgacag
     6241 ttagctctta taaatatccc tttcgtccgg aattaattcc tcccgactat atggaagcta
     6301 cctgcataga ggcagtcgaa gtggatcttc atattcgccc ctttaaagct tttatcaatt
     6361 tattcgccag aaaatcttat cacgtagaac gtttcatttc gaagactttc cgccggagac
     6421 ttatcaatat tctccaagag catgaattcg acatcgtcca actggaaacc gtctttttat
     6481 gtccctacct cccggatata aggaatcatt cgatggcccg tatcgtattg cggacgcaca
     6541 acatcgaaca ccttatttgg aagcgattat gggaaaacag caagcctgga ccgaaaaaag
     6601 tctatcttaa acatctctgg acaactttaa ggaaatacga gttggggatt ctagataaag
     6661 cagatggaat tgttgccatt actcgcaaag atgccgaatt ccttaaacca tttacacggg
     6721 ttcccatcat tgatatttcg tttgggatag atagttcgca atttcctgaa cctacttatg
     6781 acgattgtga aatcccttcg ctttttcata ttggcagcat ggattggata ccgaatcagg
     6841 aaggtattca atggtttctg tcagaagtat ggccaaaagt gtacgataat tttccttttt
     6901 tgaaatttta tcttgctggc cgaaatatgc ccgaatggct tttgaatggt ttctggccta
     6961 atgttgtagt tttgggtgaa gtggaagatg cccgaaaatt catgctgtcg aagtggatta
     7021 tggttgttcc tttgctggcc ggtagtggaa ttcgggtgaa aatcatagaa gctatggcta
     7081 atggcaggcc ggttattgcc tctcccattg gagccgaagg gatagaatat acagaagggg
     7141 aaaatatcct gctggcttct actcccgacc aatttctcga agccattcga aaatgcatag
     7201 ataacccaca gtttgctatc cagttgggac gtaatgcaca cttactcgtc atgaaagaac
     7261 atgacagacg aaaactcatc accgaactca ctcattttta ccatcgcctt gtatatactc
     7321 cttaacctat attcgccctt tctgattttt tcattcgacc aaggactcgt aattttgcaa
     7381 gaatgcaaca atgaataaag gatgaaatta ctggtgatac tttcgcgcgt gccctatcca
     7441 cttgaaaaag gagataaact cagggcttat caccaaatcc gttgcctttc gaaatatcat
     7501 caaattaccc tcatagctct taccgaccaa aaggtccatc ctcaggcaaa agataagctg
     7561 ctgaacttct gtgatgaagt ttatttcttg aaattaaata aattaggaat actacttaat
     7621 gtcattggcg ccttttttcg cggatggcca ttgcaaatag gttatttcta taataaaaaa
     7681 gtaaagaagg aaattgattc catcgtccgt caagtaaaac ccaaccatat ttattgtcaa
     7741 ctgattcgaa ctgcagaata catccatgac tttgatattc ctaaaaccat tgacttccag
     7801 gatacttttt caataggcat gaaacgtcgt tacgaaaagg ccaattttct tctcaaacca
     7861 ttcttccatc ttgaatacaa acgtctgatg cgttacgaac gaagaatatt cgacttgttt
     7921 cataatcaca ctctcatttc ctgccccgac aaagagcttt tccctcaccc taacaaatat
     7981 aaaattttta tcatcgaaaa tggagtcgat ttcgagtatt tcgaaacaaa atctccaatt
     8041 aagaaatacg aagtaatctt tacaggaaat atgggatatc cgccaaatat cgacgcagca
     8101 ttttatttag ccaacgagat tatgcccctt gtgagaaaac aattccccca ggctcattta
     8161 gttctggctg gagcgacacc ccatccaaaa atcaaaagat tagccaacac ttataccatt
     8221 gtaacaggat gggttgatga tatccgtgaa tggtacgaca cttctatgat ttttatagct
     8281 cccatgcgcc ttggaacagg cttacaaaat aaacttctcg aagctatggc catgcatcta
     8341 ccctgtatta cctcttcact ggccaatgag gccttgcatg caattcccaa tgaagaaatc
     8401 ctgataggag attcggccga agagtatgcc aatcatatca tagatttact cgtaaaccct
     8461 gcactctata ataaattaat cctcaatgga catcgattcg taatcgagaa ttacagttgg
     8521 gaaaatgcct cggaaaaatt gaatgccata ctcactcatc aaaaggattg aaaagtcaaa
     8581 taattactta taactccctc cttctctcac ataaatattt cttttcccgt aaaaaattcg
     8641 aataaaccat aagaattctg gaatagttat tataaacgat tgatacacgt tctcagaggt
     8701 ttacttctta tggtcatcgt attccttttt aaaattttcc cacgacatct taagataatg
     8761 gttttcggca aaatatacga gtattggcat catctcttcg ggttttaggt atccttcaaa
     8821 agaagtcaat ttctgattgt tttcatccag tatcacataa gaaggataat acatacgacc
     8881 ctgaagtaag gcaatagcta actggtgtgc tgattttttg ctagatggac aagtattcac
     8941 gtaaattttt ccgttatatc taatcgaatc acatcgttcg gcattcaatt ttccacaata
     9001 gaagtgttca ttcatgtaag atgctacggc tttttcggaa aaggtagtag catccatttt
     9061 tttacaccaa ccacaccaat cggtgtatac atcgataaat acctttttct ttatctttcc
     9121 ttcgtttttt gcaatggccg attcgaatgt ctgccattga acagtggatt tttcttcagg
     9181 ctgaaatgct atcgttgttg taagtaacac aacaaacaga acaagtctta attttgcaga
     9241 tttcacgaat gaatccattt ggttgtacaa aaataaatca tacatgaatc ataatggcaa
     9301 accccgtcca gttatgcatc atatttgaaa cataaaacta cccgcttaca tatcggataa
     9361 aaagaatgat atgcccgaat taacttacga atttgtaaaa tctctcccca aagctgaact
     9421 tcattgtcat cttgatggat cattaagaat gggtactatt ttagaacttg cgaaggagca
     9481 aaatattgaa ttaccagctt gggacgaaga aaacatgaaa aaaattctag ttccgcctct
     9541 cacttgcgaa agtcttgaag agtatttaaa accattcgat attcctgtat cagttcttca
     9601 gacccgtgag gcacttgaaa gggctaccta tgagctggct caagactgtg caga
//
LOCUS       DAOORU010000094         9576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_8859_length_9576_cov_4.197458, whole genome shotgun sequence.
ACCESSION   DAOORU010000094 DAOORU010000000
VERSION     DAOORU010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9576
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8859_length_9576_cov_4.197458"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PLF20_06855"
     CDS             complement(<1..1040)
                     /locus_tag="PLF20_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOX80753.1"
                     /translation="MKQQISKPQGKLGVLIVGMNGAVATTFIAGTMAVRKGMGVPVGS
                     LTQMGTIRIGRRIENNFPKIKDFLPLYELDQLVFGGWDINDESALDGTKTAEVLNEKD
                     INKVADELATLRPMKAVFDKEFVKKLDGNWVKKANTKMELMEMLRNDIRQFKADNHCD
                     DMIVIWCASTEVYTPIQEVHSSIEKFEEGMRNNNLAIAPSMLYAWAALLEGVPFINGA
                     PNLTIDTPAAFQLAEKLHVPIAGKDFKTGQTLVKTTIAPMLKMRMLGLEGWFSTNILG
                     NRDGEVLDDPGSFKTKEESKLSVIESILQPELYPELYKNYYHKVRINYYPPRKDNKEG
                     WDNIDIFGWLGYP"
     gene            complement(1058..1783)
                     /locus_tag="PLF20_06860"
     CDS             complement(1058..1783)
                     /locus_tag="PLF20_06860"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOX80754.1"
                     /translation="MNPIHNIKAILFDFGGTLDTDGVHWSEKFWEAYTSAGIPVDKSE
                     YEKAFIEAGDHLVSVIIQPHFGFKDTLAAQIELQLRYLESNHNLPPVEHYKYKNVILE
                     YSYKEASKTLEKSNQIISKLAQDYLLGVVSNFYGNMDTVLKEFHLIEYFQIVIDSARV
                     GVRKPSPDIFRLAIHQLRVPAGNTVVVGDSYDRDIVPAKQCGCITVWLEGRSWKKEEG
                     GKQADYAIHSLKELDTLIHNITL"
     gene            complement(1767..2771)
                     /locus_tag="PLF20_06865"
     CDS             complement(1767..2771)
                     /locus_tag="PLF20_06865"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOX80755.1"
                     /translation="MKKEYKINTLFFLIGIGIFTFLIIRFGIVNILNEIAQIGVWFIP
                     IIFIWMIIYSLNTIAWKIIIGNHLKVNFLQLVSLKISSFALNYITPVIALGGEPWKVM
                     NIKRLIGIERASSSVILYDLMHILSHFFFWTFTLFIIFFTVKPTLATYIVLGVVLAFL
                     ISIILIIYKWYKKGIVFSFIHFLNKIPLLKKVAAKLSKQEDTLEEIERQIVAFYNTRR
                     KEFYLSLGMEFFARIVGAIEFFLIMRAIQIDINFFEAIYISAATSLLTNLVGFVPLQL
                     GTREGSLYLVYESLKLAPAQGIVVSLATRVREFFFIFFGLILMRINPLTKPVEDESHS
                     "
     gene            complement(2768..3688)
                     /locus_tag="PLF20_06870"
     CDS             complement(2768..3688)
                     /locus_tag="PLF20_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOX80756.1"
                     /translation="MKQSELQNTFKSSDTEEAIDIYFYRRLGYRVALFSAKHKIRPNT
                     ITIISIFWGVVAGHLYYYNNIWINILGILFLIIANTLDSADGQLARMTNDKTELGRIL
                     DGLAGNLWFLSIYIHIGLRLTHNGYGSWIWIIGIVTGISHIFQAAIADYYRNGHLYFI
                     KGEAGSEFHSSKDIKRQYDSLSWKKDFWMKLFMYFYHNYTREQELFTGNLQKLIGCVQ
                     KNFQGQIPQWLRDDFRKENKPLMKYTNILSFNTRAITLWISLLINLPLLYWFFELIVL
                     NIILIYMVLKQQFISKKYLNLLQKEVLQNT"
     gene            complement(3685..4536)
                     /locus_tag="PLF20_06875"
     CDS             complement(3685..4536)
                     /locus_tag="PLF20_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80757.1"
                     /translation="MNLHFAAISRGKRYSPNHITNDARILQLTIEELEKQGHSVTVYD
                     EDEIRPDLIDTPYIFNMIRGTRSLGVLQELEANGALVINSTEAILNCYRKNLATLLPP
                     ANIPIPSSIVIDTYMPLPEIPFASNPFKVWIKRGDVHAIHREDVTTVYSIEELNEIIL
                     EYRQRGIHSAVIQQHVDGPVIKFYAVRNTPFFHWYFHDSTFTMQINEIKLKYLAAKCA
                     ETLKVDVYGGDAIVSPDGKIQIIDLNDWPSFAPVRDEAAKYIAQVIINKAYSHSVELN
                     LHSFPSI"
     gene            complement(4580..5179)
                     /locus_tag="PLF20_06880"
     CDS             complement(4580..5179)
                     /locus_tag="PLF20_06880"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX80758.1"
                     /translation="METKQKIIDTAIELFQQNGIKSVTMDWIASEAGVSKRTIYEIFT
                     KKAELLKACMDKITEENLNALHQIRQNSTCFFETIKQLEEFHIQSLQRFHPNFFLDVQ
                     KSFPSLWKKMNDQNDTMKMSEIMYFLKESIMNGLIRADLHITFISRVILELFKLAFSI
                     ELDNDTRIDRKNIYKDLIINYFRGIATDKGLKELEQLEH"
     gene            complement(5337..6041)
                     /locus_tag="PLF20_06885"
     CDS             complement(5337..6041)
                     /locus_tag="PLF20_06885"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOX80759.1"
                     /translation="MKFAIIAAGEGSRLRKEGLYIPKPMIEIGGMTLIDRLIDRAKRY
                     EFSTACIIVNDIYPELANWFRDKDYGIFIQLIVKSTPGSMHSFYELSQFLRDDDFILT
                     TVDPIYTDESFRYFLEFIDTHPHADGIMAATNYVDDEKPLWIICNNRMQIVEFSDTQK
                     ESSLVSAGFYYFKPLVINFLEKAMQQGMFRMRDIQRFLLKQGCSLIACNVGKVIDIDH
                     TSDILQAELMLKSKNS"
     gene            complement(6053..6889)
                     /locus_tag="PLF20_06890"
     CDS             complement(6053..6889)
                     /locus_tag="PLF20_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOX80760.1"
                     /translation="MVSNFITISIVGLLAGFIFSVPVAGPISILITSNALKGKFWYSL
                     KAAIGASFADFFYVFISMLGLTHFYEWYKPAIPYILLVGAVFVFVIGFRITKTRLKVE
                     NLNDNSILKSKYHNRGGFRTGLFLNFLNPTLFFGWLSSTFITFSLVASLGFNMGGLED
                     SFQESINAVDHSMLQKPSLFTPSLKDSLSYNAPESVEHEAYTTAKAPVTRWYHYTLSA
                     LYAFFVSIGSITWFYIYVSFLVKNRHRINISTLTNVIRGLGFILIGISIYLLGRGIDL
                     LT"
     gene            complement(6903..7646)
                     /locus_tag="PLF20_06895"
     CDS             complement(6903..7646)
                     /locus_tag="PLF20_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HOX80761.1"
                     /translation="MMKAVILAAGLARRLKPITNYTPKCLLKIRNKTILGMILDNLIE
                     NQIFQIVIVTGFESAQIQQYVKKNYPQLNIEFVYNSDYEFTNNAYSLLLTQKAIEGHS
                     LLLLDGDIVFDPLIIDKLVHCKQENSLALNKAHKNFLSSEEIKVRITKDNYIVEISKD
                     INPILAAGESAGIQLFSVATTNKLYQILHQQIDYQQHINEFYESAFEELIHRGEKIYA
                     MDIAPFQSMEIDTETDWRKANNSFNINIL"
     gene            complement(7659..8555)
                     /locus_tag="PLF20_06900"
     CDS             complement(7659..8555)
                     /locus_tag="PLF20_06900"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HOX80762.1"
                     /translation="MLVSTREYKASLKALEIEEYFDLWIYRPLGYIVAKLVQSISVTP
                     NQLTVLAMFCGIASGYVFSLGTQQAFILGALLYFMFNILDCSDGQLARIKGTSSVAGK
                     ILDGAADYIAGIAIYIGIGIGYVEYVSNPVGWWILLALAAISNIVHSIVIDSERYRFT
                     HHAWGRPHQLTNDYEYYKEELNRLRKIPGTCFERFVLQTYLVYLNISRFFHKSPKNQY
                     FIYDPGIYYRKFRKMMKAWTFLGPTTHITIIIVCCLFMRPDIAAWIIVVPMNIYWLLL
                     VWIQNKKLKTLHFITNPNPTKN"
     gene            complement(8753..>9576)
                     /locus_tag="PLF20_06905"
     CDS             complement(8753..>9576)
                     /locus_tag="PLF20_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80763.1"
                     /translation="VSSALFAQVENWAKSMGMKAVQGPLGFSDMDKEGMLIEGFEELG
                     TIATYYNHPYYPIHTEKAGYQKDVDWLEYELNAEVTVPEKFIRVGQIAQERYKLSIVQ
                     FKKKKDLLKYAHQIFEVLNESFRPLYGFAPLTDAQIDYYIKMYFSFIVLDFISVVVDE
                     HDKVAAFGITMPSLSKAVQKSGGKILPFGFIHILRAMKKNDTVDFYLTAVRPELQGKG
                     VNALLFIDLIPKYRKFGIKKAESNVELENNQMVRRQWDMFPKRQHKRRRAYIKHI"
BASE COUNT         2862 a         1809 c         1895 g         3010 t
ORIGIN      
        1 ggataaccca gccaaccaaa tatgtcaata ttgtcccagc cctctttatt atctttacga
       61 ggtgggtaat aattgatgcg aactttgtga tagtagttct tgtataattc ggggtagagt
      121 tcgggttgca gaatgctttc tattacactg agtttacttt cttccttggt tttgaaagaa
      181 ccaggatcgt cgagtacttc gccatcgcga ttgcccagga tattggtcga gaaccagcct
      241 tcgaggccta gcatgcgcat tttcaacatg ggtgcaatag tggttttcac taaggtttgg
      301 cccgttttga aatctttccc tgcaatggga acatgtaatt tttctgccaa ctggaaagcg
      361 gcaggtgtat cgatggtaag gttgggagct ccattgatga atggaacgcc ctcgagcagt
      421 gctgcccatg catataacat actcggagca attgccagat tattgttgcg cataccctct
      481 tcaaactttt ctatactcga atgcacttct tgtataggag tataaacctc ggtactggcg
      541 caccaaatca caatcatatc atcgcaatga ttgtcggctt tgaactggcg aatatcgtta
      601 cgcaacatct ccatcagctc cattttggta ttggcttttt ttacccaatt cccatcgagt
      661 ttttttacga attccttatc gaatacggct ttcatgggac gaagagtagc taattcatcg
      721 gctaccttat taatatcttt ttcgtttaaa acttcagcgg tttttgtacc atcaagagct
      781 gattcgtcgt tgatatccca tcctccgaaa accaattgat ccaattcata caagggtaaa
      841 aagtctttga ttttgggaaa attgttttca atccgtcttc caatgcggat agtccccatt
      901 tgagtgagag atcccactgg aacacccatt ccttttctta cagccatcgt accggctata
      961 aatgtcgtag caactgcgcc attcattcct acgatcaata ccccaagttt tccttgaggc
     1021 ttgctaatct gttgtttcat gatgattaaa tttaatttta aagggttata ttatgaatta
     1081 aagtatcgag ttcttttaga ctatgtattg cataatcggc ttgcttgccg ccttcctcct
     1141 ttttccagct ccttccctcg agccaaacgg taatgcaacc gcattgcttg gccggcacaa
     1201 tgtcacgatc ataagaatca cctacaacga cagtatttcc tgcaggcact ctcagttgat
     1261 gaatggcaag tcgaaaaatg tcaggactgg gtttacgaac gcctaccctt gccgaatcta
     1321 tcacaatttg aaagtattca ataagatgaa attcctttaa aactgtgtcc atattgccat
     1381 aaaagttgga tactacgccg agaagataat cttgggcaag ttttgaaata atttgattcg
     1441 atttttccag ggttttacta gcttctttgt acgagtattc taagatgacg ttcttgtact
     1501 tgtaatgttc aaccggaggc aggttatgat tcgattctaa gtaacgtaac tgaagttcta
     1561 tctgggcagc taaggtatct ttgaaaccaa aatgaggctg aatgataacc gaaacgagat
     1621 gatctccagc ttcgatgaaa gccttttcgt attcggactt atcaacgggt atacctgccg
     1681 atgtatatgc ttcccagaat ttttcgctcc agtgcactcc atccgtatcg agtgttcctc
     1741 caaaatcaaa caaaatggcc ttgatattat gaatgggatt catcttctac gggttttgtc
     1801 aaaggattga ttcgcataag gatgagtccg aaaaagataa agaaaaattc tcgaacacgt
     1861 gtagccagac ttacgacaat accttgtgca ggggctaact ttagactttc gtatactaaa
     1921 taaaggctcc cttcgcgagt tcctaactgc aagggtacaa aacctactaa attggttagc
     1981 agcgaagtgg cggcactaat ataaatggcc tcgaaaaaat tgatatcgat ttgaatagcc
     2041 cgcatgataa gaaagaactc gatcgcacca acaattcgtg caaaaaattc catcccaagg
     2101 cttaaataaa attcttttcg gcgagtatta taaaatgcca caatctgtcg ttctatctct
     2161 tcgagcgtgt cttcctgctt cgacagcttg gctgcaactt tcttcaaaag cggaatctta
     2221 ttcagaaaat gaatgaaaga aaatactatc ccttttttat accacttata aataatcagt
     2281 attatgctga tgagaaaagc aagtaccacg cccagcacga tgtaggtagc caaggtaggt
     2341 ttcactgtaa agaagataat aaagagggta aaagtccaga agaagaaatg ggaaagaata
     2401 tgcatcaaat cataaaggat gactgatgaa gaagctcttt cgattcctat taaacgtttg
     2461 atgttcatta ctttccatgg ttctccgcct agggcaatca ctggagtgat gtaattgagt
     2521 gcaaatgaac tgattttaag agagaccaac tgaagaaaat ttaccttcaa gtggtttcct
     2581 atgatgattt tccaagcaat agtattcaaa ctataaataa tcatccagat gaagatgatg
     2641 ggaataaacc atacgcctat ttgagctatc tcattcagta tgtttacaat accgaatcga
     2701 ataatgagaa aggtaaaaat gcctatccca ataagaaaga acagcgtatt tattttgtat
     2761 tcctttttca tgtgttttgt aagacttcct tttgaagcaa gttcaggtat ttcttactga
     2821 tgaattgttg ctttaaaacc atgtatatca ggataatatt caatacaatg agctcaaaaa
     2881 accaatagag caaaggtagg ttaatcagca atgatatcca taatgtaata gctcgtgtat
     2941 tgaatgataa tatattggtg tatttcatca aaggcttgtt ttctttgcga aaatcatcgc
     3001 gaagccattg aggtatctgg ccttgaaaat tcttctggac gcagccaatg agtttttgta
     3061 gatttcctgt aaaaagttct tgttctcggg tatagttgtg ataaaaatac atgaagagtt
     3121 tcatccagaa atcttttttc cacgaaagtg agtcgtattg tctttttata tccttacttg
     3181 aatggaattc gctgccggct tcgcctttaa tgaagtaaag atgtccgttt cggtaataat
     3241 cggcaatagc tgcctgaaag atatgcgaga taccagtaac gataccaatg atccagatcc
     3301 atgaaccata gccgttgtgg gtaaggcgga gcccaatgtg aatgtaaatg ctgagaaacc
     3361 atagattgcc tgccaaacca tcgagtattc tgcccagttc ggttttatcg tttgtcattc
     3421 ttgcaagctg gccgtcggca ctgtcgagag tatttgcaat gatcaggaaa agaataccga
     3481 gaatattgat ccagatgttg ttgtaataat aaagatgacc cgccacaaca ccccaaaata
     3541 tgctgataat ggtgatggta ttcggacgta tcttgtgctt tgccgaaaat aaggctaccc
     3601 tgtaacctaa tcttcgataa aagtaaatat cgatcgcttc ctcagtatcc gatgatttaa
     3661 aagtattttg taattcgctt tgcttcatat actcggaaaa ctgtgcaagt tgagttctac
     3721 ggagtgtgaa taagctttgt ttataattac ttgtgcgatg tatttagcag cttcgtcacg
     3781 tacaggagca aaactcggcc aatcattcaa gtcaataatt tgaatcttac catcgggcga
     3841 aacaatagca tcacctccat atacatcaac ctttagggtt tcggcacatt tggctgccaa
     3901 atatttcagt tttatttcat taatctgcat ggtaaaggta ctatcatgaa aataccagtg
     3961 aaaaaacggt gtattcctta ctgcataaaa ttttataacc ggtccatcta catgttgttg
     4021 aatcactgca ctgtgaatgc ctctttgtcg atattcgaga atgatttcgt ttaattcttc
     4081 gatactgtat acagtggtta catcctcgcg atgaatggca tggacatcac ctcgttttat
     4141 ccataccttg aatggattgg aggcaaaagg aatttcgggc agaggcatat aagtatctat
     4201 cacaatggag gagggaatgg gaatattggc aggaggtaat aaagtggcaa ggtttttacg
     4261 gtagcagttg agaattgctt cggtagaatt gatgaccaaa gcgccattgg cttccaattc
     4321 ctgcaatacc ccgagcgacc gagtgcctcg tatcatattg aaaatatagg gtgtatcgat
     4381 caaatcgggt cttatttcat cctcgtcgta aaccgtaaca ctatgcccct gcttttcaag
     4441 ttcttcgatg gttaattgaa gaatccgagc atcgttggta atatgattgg gtgagtatct
     4501 tttccctctc gatatggcgg caaaatgtag attcataatc gctgatttag ttgtaagcag
     4561 aatttgaaaa ttgaacttcc taatgttcta attgttcgag ttctttcaat cctttgtcag
     4621 ttgctatccc tctaaaatag ttaataatca agtctttata aatatttttt ctatcgatgc
     4681 gagtatcatt atctaattca atagaaaagg caagtttaaa aagttcgagt ataacccggc
     4741 taatgaatgt gatgtgaaga tcggctctaa tcaagccatt catgatgctt tccttcagaa
     4801 aatacataat ttcactcatt ttcatggtat cgttctgatc gttcattttt ttccacaggc
     4861 ttgggaaaga tttctgaaca tcgagaaaaa aatttggatg aaaacgctgt aacgattgta
     4921 tgtgaaattc ttcgagttgt ttgatagttt cgaaaaaaca agtggaattt tgtcggatct
     4981 gatgaagtgc attgaggttt tcctctgtaa ttttatccat acaggctttc aaaagttcgg
     5041 cctttttggt aaaaatttcg taaatggtac gtttggaaac tcctgcttcg cttgctatcc
     5101 aatccatcgt aactgatttg attccatttt gttggaacag ctcaatggcg gtatcaatga
     5161 tcttttgctt tgtttccatt ccatcatcac gattggaggc gtaaaagtac aaaacttttt
     5221 cattttgaaa agttttcttt acgaaatagg taagtatagc taaagttcaa tgactgctaa
     5281 attaggttag attattctat attagatatt taagatacta taaaccattt taaattttaa
     5341 ctattttttg attttaacat gagttcggct tgaaggatat cgcttgtatg atcgatgtct
     5401 attaccttgc caacgttgca agcaatcaga gaacatccct gttttaaaag aaaccgctgg
     5461 atatcgcgca tacgaaacat accctgttgc attgctttct cgaggaagtt tataacgagg
     5521 ggcttgaaat agtaaaaacc ggcactcact aacgacgact ctttttgcgt gtcggaaaat
     5581 tctacaattt gcattctatt gttgcagata atccaaagtg gcttttcgtc atctacataa
     5641 tttgtggcag ccattatgcc atcggcatgt ggatgagtgt cgataaactc tagaaagtat
     5701 ctgaacgatt cgtcggtata aattggatcg actgtagtca ggatgaaatc atcatcgcga
     5761 agaaactgac taagttcata aaaactatgc attgaacctg gagtcgattt gacaattaac
     5821 tggatgaata tcccgtaatc cttatctcga aaccagttcg ccagttcggg atagatatcg
     5881 tttacgatga tgcaggcagt tgaaaattca tatcgtttcg cacgatcgat caaacggtct
     5941 atcagtgtca ttccaccgat ttcgatcatg ggcttgggaa tgtaaaggcc ttctttacgc
     6001 aggcgactgc cttcacctgc tgctataatg gcaaatttca tcgacgagta gattaagtga
     6061 gtaggtcaat accgcgacct aaaagataga tagatatacc tatcaatata aaacccaggc
     6121 cgcggataac atttgtaagt gtgctgatat tgatgcgatg ccggtttttt actaaaaacg
     6181 agacataaat gtagaaccat gtaatactac caatggatac aaaaaacgca tacaaggccg
     6241 ataaggtgta atgataccag cgtgtaacgg gagctttagc cgtagtgtag gcttcatgtt
     6301 ctaccgattc cggggcattg taagaaaggc tatcttttaa ggaaggtgta aatagagagg
     6361 gtttttgtaa catggaatga tcaactgcgt ttatgctttc ttggaaagaa tcttccaatc
     6421 ctcccatgtt aaaaccaaga gaagctacaa gcgaaaaagt tataaaagta gaggaaagcc
     6481 atccaaaaaa aagtgtgggg tttaagaaat taaggaacaa tcctgtgcga aatcctcccc
     6541 gattatggta tttacttttc agaatagaat tatcgttaag gttttcaact ttcaacctgg
     6601 ttttagtaat tctaaatcca attacaaata cgaaaaccgc tccaaccaat agaatatatg
     6661 gaatagcagg tttataccat tcgtaaaaat gggttaagcc gagcatggag ataaaaacat
     6721 agaaaaaatc ggcaaacgaa gctcctatgg ctgctttaag gctataccaa aattttcctt
     6781 tgagagcatt ggaagtaatc aaaatactga tagggccagc aaccggcact gaaaatatga
     6841 atcccgccaa taaacctaca atagagatag tgataaaatt gctgaccatt ttttaattcg
     6901 atttataaaa tattgatatt aaatgaattg ttcgctttgc gccaatcggt ttcagtatct
     6961 atttccatgc tctggaaagg agcaatatcc atagcataaa ttttttctcc ccgatggatt
     7021 aattcctcga atgccgattc ataaaattca ttgatatgtt gttgataatc aatctgttga
     7081 tgaagaatct gatagagctt gtttgtagta gctaccgaaa aaagctgaat acctgccgat
     7141 tctcctgcgg ctaaaatcgg attgatatct ttactgattt caacgatata attgtctttg
     7201 gtaattctca ccttgatttc ttcactgctt aaaaagtttt tatgggcctt gttcaaagcc
     7261 agagaatttt cttgtttaca atgaacaagt ttatcgataa tcaaaggatc gaaaacaata
     7321 tctccgtcga gcaaaagtaa gctatgtcct tcgatagctt tttgggtaag tagtaaagag
     7381 taagcattgt tggtgaattc ataatcggaa ttatatacaa attctatgtt gagttgagga
     7441 tagtttttct ttacatattg ttggatttga gccgattcga agccggtaac gattacgatt
     7501 tgaaaaatct ggttttcgat caggttgtcg agtatcattc ccaaaatggt cttgtttctg
     7561 attttgagca aacatttggg agtataatta gtgatgggtt ttaaccttcg tgctaatcct
     7621 gcagctaaaa ttacggcttt catcatattt tgtaaatctt agtttttagt tggattgggg
     7681 ttagtaataa aatgaagtgt ctttaacttt ttattctgaa tccacactaa gagtaaccag
     7741 tagatattca taggtactac gatgatccag gcggcaatgt cgggtcgcat gaaaagacag
     7801 cacacaataa tgatggtgat atgagttgtg ggacctaaga atgtccaggc cttcatcatt
     7861 ttgcgaaatt ttctgtaata aattcccggg tcatatataa aatattgatt tttaggtgac
     7921 ttatggaaga atcggctaat gttcaaataa accagatagg tttgtaaaac gaagcgttcg
     7981 aaacaggtac ctgggatttt tcttaaacga ttgagctctt ccttataata ctcgtaatcg
     8041 tttgtcaatt gatgaggtct tccccaggca tgatgtgtaa aacggtaacg ttccgaatcg
     8101 attacaatgg aatggacaat attggaaata gcagccagtg ctagcagtat ccaccatcca
     8161 actggattag aaacatattc gacatagcct attcctatac caatatagat tgcgatgccg
     8221 gcgatataat cggcggcacc atccagaatt ttacctgcaa ccgatgatgt tccttttatt
     8281 cttgccaatt gtccatcgct gcaatctaat atattgaaca taaaatagag caaggcaccc
     8341 agaataaatg cctgctgggt tcccagggag aagacatatc cgctggcgat accgcaaaac
     8401 atggctaaaa ccgtaagctg gttgggtgtt acacttattg actgaaccaa ttttgccact
     8461 atatatccta aaggccgata aatccataaa tcgaaatact cctcaatttc taaagccttg
     8521 agtgaagcct tatattctcg ggtacttaca agcattgttt atggttcgaa ttgatgtggt
     8581 ctacaaaaat ataaaatttc acaaaatctc attattcgtc agctcttggg gaaacatcat
     8641 ctcgtcgata tttcattgta aactgatgat tattatttga aaaggaattg attttttgtt
     8701 tggcaaatct gttcactgaa gttttgtttc gattcattgg tcgtacaggt gatcagatgt
     8761 gctttatata ggctcgtctt cgcttgtgtt gtcttttggg gaacatatcc cactgccgcc
     8821 ttaccatttg gttattctcg agctcaacat tgctttcggc tttttttatc ccaaattttc
     8881 tgtatttggg aataaggtca ataaacaata gtgcatttac tccttttccc tgaagttctg
     8941 gtcttacggc tgtcaagtaa aaatcgaccg tgtcgttttt cttcatggct cgtaaaatat
     9001 ggatgaagcc aaaaggcaga atcttgccac ctgacttttg tacggccttg ctcagtgatg
     9061 gcatggtaat gccaaaagct gcaactttgt cgtgttcgtc aacgacaacc gaaataaaat
     9121 cgagtacgat gaacgagaaa tacattttga tgtagtagtc gatttgagcg tcggttagag
     9181 gggcaaaacc atacaaggga cggaaggact cgttaagaac ctcaaagatt tggtgtgcat
     9241 atttcagcag atccttcttc tttttgaatt gtacaatact gagcttgtag cgttcctgag
     9301 caatctgtcc tactctgatg aacttttcgg gaacggtaac ctcggcattc agttcatatt
     9361 cgagccaatc gacatctttc tgataacctg ctttttcagt atgaatggga taatagggat
     9421 gattatagta agtagcaata gtccctaatt cttcgaagcc ttctatgagc ataccttcct
     9481 tatccatgtc ggaaaatccc agtggtcctt gtacggcttt cattcccatg ctttttgccc
     9541 aattttcgac ctgagcaaat aacgcagaag atactt
//
LOCUS       DAOORU010000095         9426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_9035_length_9426_cov_5.751254, whole genome shotgun sequence.
ACCESSION   DAOORU010000095 DAOORU010000000
VERSION     DAOORU010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9035_length_9426_cov_5.751254"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..2059
                     /locus_tag="PLF20_06910"
     CDS             392..2059
                     /locus_tag="PLF20_06910"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007653523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HOX80764.1"
                     /translation="MKTDIEIAHEVKLKHINEIAGMLGINPEDLVLYGKYKAKIPLNY
                     INYDKIKGNKLVLVSAISPTPAGEGKTTVSIGLAQALNRIGKKTTAVLREPSLGPVFG
                     VKGGATGGGYSQVLPMEDINLHFTGDFAAIERAHNLLAALIDNNIQSKKRSLGIDPRT
                     VKWKRVMDMNDRSLRRIIVGLGGTTNGVPRETGFDITAASEVMAILCLAENLQELKNR
                     LGNIFIGYTYDKKPIFARDLNANGAMTALLKDAFMPNLVQTLEGTPAIIHGGPFANIA
                     QGTNTVVATKMGLSLSDFVVTEAGFGFDLGAEKFIDIKCQYAGLFPCSVVLVATVRAL
                     KYHGGKPLKDLSQPDIEALKKGIGNLEKHIENMKLFGIHPIIALNRFISDTQEELDYI
                     VQVCKNLKVEVAVVDVWGKGGEGALNLATIVEHVATKCPNHHNTLYDWNWTPEQKIET
                     IAKKIYGANAVDYTAQAKSDMEKVYSLGLDKLPVCVAKTQKSLSDNPELLGRPKDFVI
                     TVREVEIASGAGFIIPITGQIMRMPGLPDEPAAERIDIDENGRIIGL"
     gene            2577..3800
                     /gene="mnmA"
                     /locus_tag="PLF20_06915"
     CDS             2577..3800
                     /gene="mnmA"
                     /locus_tag="PLF20_06915"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOX80765.1"
                     /translation="MGKKSSKTKKVAIGLSGGVDSSIAAWLLLQEGYDVFGIFMINWH
                     DKAGLLQGDCPAEHDLIITQLVARHLDIPLHVIDFSEQYRERVVNYMFEGYRRGLTPN
                     PDILCNREIKFDLFIDKARELGADYIATGHYCRKNIIEVNGEPVYRLLSGIDKNKDQS
                     YFLCQLNQEQLSQTLFPIGELTKPQVREIARKLNMPTADRKDSQGICFVGKVDLPVFL
                     QQKLEPKEGKIIEIDKNFPYPSLRAFNSEICDINNLKLWSVDYIYHPEDGIVVGLHHG
                     AHFYTIGQRKGLNVGGKAEPLFVLSIDIKENVVYVGQGQDHPGLYRRGLFIKIEDVHW
                     LRPDLKMQEGEIRDYLVRIRYRQPLQTAHLFMQNNGLYIIFDKMQRGITPGQFAAWYN
                     EEELIGSGPISNEME"
     gene            complement(4012..4085)
                     /locus_tag="PLF20_06920"
     tRNA            complement(4012..4085)
                     /locus_tag="PLF20_06920"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4049..4051),aa:Ala,seq:cgc)
     gene            complement(4308..5423)
                     /locus_tag="PLF20_06925"
     CDS             complement(4308..5423)
                     /locus_tag="PLF20_06925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOX80766.1"
                     /translation="MADKQPIKVLHVSTQTGWRGGEQQIMYLVRLGKEVRHYLFCPEE
                     AALRKEAKKEEITIKTFDSSSKFSFFLKVPFQLWQFISEVRPHLIHVHDSGAHTLAYL
                     LARRKELALPLIVSRRVDIRRGKNIFSRKKYNQPEIAKIFCVSNYVKTIMESVVKDRN
                     KLLTIYSGIDLQRFLYRSPYPKLRNELDIPDDYLIVGNIAALTKPKGYPVFVKTAAEL
                     LKHRQNFIFVIAGDGPMRANIEKMIQNLGLEKNVILLGFRDDIPEILPSLDILLFPSE
                     NEGLGTTILDAMACGVNVVATSAGGIPEMITHQMNGLLAPVGDIQQLASNVLRLACNA
                     ELRNMLKENAYITVKNFSVENMVTQTIKAYHEIPGTI"
     gene            complement(5402..6163)
                     /locus_tag="PLF20_06930"
     CDS             complement(5402..6163)
                     /locus_tag="PLF20_06930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOX80767.1"
                     /translation="MEALSVVIITYNEERNIERCIRSVVGIADEIVVVDSFSTDRTRE
                     IAGEFGAKVIKHEFEGYVQQKNYALAQATHRWVLSLDADEALSEKLRNSIKEIKFNPS
                     ADGYTMNRLTNYCGKWIRHSGWYPDKKLRMVLKKKAYWTGTNPHDKLELFESKNIRHI
                     PGDILHYSYYSLEDHLQQIDRFMQIRAYELFEKRIKPNVWHFYLKPLHKFFKHYFFQL
                     GFMDGYEGYQIARLSAFGIFIRYAKLRELWRTNNP"
     gene            6669..7802
                     /locus_tag="PLF20_06935"
     CDS             6669..7802
                     /locus_tag="PLF20_06935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HOX80768.1"
                     /translation="MIFLGLGAFCIFCFAALLDPFLNLWDERFHALVGKNLMKHPLMP
                     TLYDDPIVQMEYDKWDRYHIWLHKQPLFLWQIALCFKLFGISEFALRLPSVILGTILV
                     LITYRSGKLLVNQRVGYIAAILTLSTLYIIELIAGREELDHNDVSFLVYISLSIWSLI
                     EYQFTHKKIWIYLIGLFSGMAILCKWLVGLVVYLGWIILRGVEKKFKVTENKDILLSL
                     LITIIISAPWQIYILLKFPNEAIASYKYNALHFKTALEGHGGDLFYHFKMMTRIYGSW
                     SKYLIIPAFITFFIKSKDKKLFYSLSSIVIIVYVFFSLAATKMPSYTIIVVMINFIAF
                     ATLIDFILNYISHFIKNPLVNHLIFAASILMIFTLKFDINTLR"
     gene            7842..8114
                     /locus_tag="PLF20_06940"
     CDS             7842..8114
                     /locus_tag="PLF20_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80769.1"
                     /translation="MMINNKKIFTSLKLPPNSVLFNVEGRYYVDAMFYTGLPAYNFLP
                     STDQCNDLTKKGKQIVILNSNNKEIPAYYKNDPTIMFIKEELICHE"
     gene            8385..9092
                     /locus_tag="PLF20_06945"
     CDS             8385..9092
                     /locus_tag="PLF20_06945"
                     /inference="COORDINATES: protein motif:HMM:NF017395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOX80770.1"
                     /translation="MLIFLHIDLTKNLKYDRITKRIIKNSLKYNSNCIDIGCHKGQIL
                     DVMIQYAPAGKHYAFEPIPYLFDELQIKYKNKAVVYPFALSDTSGKSSFQLVKNAPAY
                     SGIKKRHYDISNPDIEEITVKLKTLDEIIPENEIIDFIKIDVEGAEFCVLKGGKNLLK
                     KNKPIILFEFGKGASDYYGTKPSDLFSYLTEQIGLKIFTLDAYLKSKRNISQSEFEHL
                     YISGKEYYFIAASDIKE"
BASE COUNT         3100 a         1731 c         1691 g         2904 t
ORIGIN      
        1 gaatcgaacc tggatttaaa gtttaggaaa cttccgttct atccgttgaa ctatggggcc
       61 caaagtgggc ggcaaagata cagagttttt aacaaatctt ttatcaatta cccaagtaaa
      121 tatacttatg ataaaacaaa tcaactcgac tttgcctcac aaattgtgaa ttaagataaa
      181 agctatcatc aggaccgtct gaatgaatac tcttcttctg attccataaa tcttataaaa
      241 ctaaaaaaaa tctttctcgt tttgagccaa aaataagagt acttttgcct ctctaagata
      301 aggatatcta tacattgata attaaaatgc aaataattcc gaaactgagg ggaaagtatt
      361 aataaataaa taatattaaa actctatcca tatgaaaaca gatattgaaa ttgctcatga
      421 agtaaagttg aaacacatca atgaaattgc aggaatgcta ggaatcaatc ccgaagatct
      481 ggttctctac ggtaaataca aagcaaaaat cccattaaat tatatcaact atgataaaat
      541 aaagggaaac aagctggttc tggtatctgc catttcgccc acgcctgcag gcgaagggaa
      601 aactaccgtc tcgattgggc tggctcaagc cttgaaccgt attggcaaaa agaccactgc
      661 agtattgcgt gagccttcat taggtccagt atttggtgtg aaaggaggag ctacaggtgg
      721 ggggtattca caggtccttc ccatggaaga tatcaatctg cattttacgg gggattttgc
      781 tgcaatcgaa agagcacaca atcttcttgc agctttaatt gataataaca tccaaagtaa
      841 aaagcgctcg ctgggaatag atccacgcac agtgaaatgg aaaagagtaa tggatatgaa
      901 cgaccgttcg ctgcgacgta tcattgtggg cttgggtggg accacgaatg gggtacctcg
      961 tgaaacgggc ttcgatatta ctgcagcctc cgaagtaatg gccatccttt gcctggccga
     1021 aaatctccag gaactgaaaa atagactcgg caacattttc attggttaca catacgataa
     1081 aaaacccatc tttgctcgcg acttaaatgc aaatggcgca atgacagcct tactgaaaga
     1141 tgcctttatg cctaacctcg tgcaaactct cgaaggcact ccagccatta ttcatggagg
     1201 accatttgcc aatattgccc aaggaaccaa cacagtagta gctaccaaaa tgggactatc
     1261 cttaagtgat tttgtcgtta cagaggctgg tttcggtttt gacttgggtg cggaaaaatt
     1321 tatcgacatc aaatgtcaat atgccggctt gttcccatgc tcggttgttc tcgtggctac
     1381 ggtgagagca ctgaaatatc atggtgggaa accattgaaa gacctttccc aacccgacat
     1441 tgaagcgctg aaaaaaggca tcggaaatct cgagaaacac atcgaaaaca tgaaactgtt
     1501 cggcatccat cctattatag ctcttaaccg ctttataagc gacactcagg aagaattgga
     1561 ttatatcgtt caggtatgca aaaatctgaa agtggaagta gctgtagtag atgtttgggg
     1621 aaaaggagga gaaggggctc tgaatttagc gactatcgtt gaacatgttg ctactaaatg
     1681 ccccaatcac cataatactt tatatgattg gaactggaca cccgaacaaa aaatcgaaac
     1741 catagccaaa aaaatttatg gggcgaatgc agtggactat actgcacaag caaaatcgga
     1801 catggaaaag gtctattctc tcgggctgga caagctgcct gtctgcgttg caaaaaccca
     1861 gaaatcactt tccgataacc ctgagttgct tggccgacct aaagatttcg tgattaccgt
     1921 gagggaggtg gaaattgctt caggggcagg atttatcatt ccaattacag gtcaaatcat
     1981 gcgtatgcca ggcctgcctg atgaacccgc cgcagaaaga atagatatcg acgaaaacgg
     2041 taggataata ggtctctaaa cttgacccat ttgcaaacac taaatgttat tattcagaac
     2101 ccctgattcg attttaatca ggttgtcgtg attttgatga ttgcattata gtattactta
     2161 ctctaaataa tcactcttat caccctcttg aatataatct cattatcagc aaccgctaat
     2221 tcctttctta aattcctttc ttgatgtttg tatctgaaag gttctttaca accaggcaaa
     2281 aaaattgttg cacgaagagg tgcaaagcag gctaaggatt cgaaatgatt attcttcgcg
     2341 atctttgcgt cttagcgaaa aatttatgca cgcagagtcg aaaaggacgc caaagaatag
     2401 actgcttatt ctttgtagcg ttctttgcat ttctaagact gtgcttttat aaaatattga
     2461 tcggcaatta taaagtcaaa agttctgaag ttgataaaat attttcaaaa atacagctaa
     2521 acaatttgtt caatgaataa gtctactgaa aatcagagta attttgcagc tgttttatgg
     2581 gaaaaaaaag ttcgaaaact aagaaagtag ccattggcct ttcgggtgga gttgattcca
     2641 gcattgctgc atggcttcta ttgcaggagg gatatgatgt ttttggaatt tttatgatca
     2701 attggcatga taaagctggt ttgcttcagg gagattgccc tgccgaacat gatctcataa
     2761 ttactcagct tgtagcccgt catttggaca ttcccttgca tgtaatcgat tttagcgaac
     2821 aataccgcga aagagtcgta aattatatgt tcgaaggata ccgtcgagga ttgacaccta
     2881 acccggatat cttgtgcaat cgtgaaatca aattcgactt atttatcgat aaagcccgtg
     2941 agcttggcgc tgactacata gctacgggcc attattgccg aaaaaacatt atagaagtga
     3001 atggagaacc tgtttaccgt ttattgtcag ggatagacaa aaacaaagat caaagttatt
     3061 ttctttgcca actaaatcaa gaacagctca gccagactct tttccctatt ggcgaactta
     3121 ccaagcccca agtaagagaa atagccagaa aactgaatat gccgacagcc gatagaaaag
     3181 attcccaggg tatatgtttt gtagggaaag tggaccttcc tgtatttctt cagcaaaaac
     3241 tcgagccgaa agaagggaag atcatcgaaa tcgataaaaa ttttccttat ccatcattac
     3301 gagctttcaa ttcagaaata tgtgatataa ataatttaaa actttggtca gtagattata
     3361 tctatcatcc agaagacggc atagtagtgg gtttacacca tggagctcat ttttatacca
     3421 ttgggcagag aaagggcttg aacgttggcg gaaaagccga acccctcttc gttctttcca
     3481 tcgatatcaa agaaaatgtt gtttatgttg gacagggtca ggatcatcca ggcctgtacc
     3541 gaaggggctt gtttattaaa attgaagacg ttcactggtt gcgtcctgat ctaaaaatgc
     3601 aggaaggcga aataagggac tacctggtta gaataagata caggcaacct ctacagactg
     3661 ctcacctctt tatgcaaaac aacgggcttt atattatttt cgacaaaatg caaagaggaa
     3721 ttactccagg gcaatttgcc gcatggtata acgaagaaga attgataggc agtggcccta
     3781 ttagcaatga aatggaataa tcgatcaaac ttcatgaaac ctcttcatgc aaggaacgaa
     3841 ttccctcttt cttggcttca aaatatgact ctgactctaa tccatccccc acctgatgtt
     3901 taatatagtg gcaaaaaaaa tatcaaaact ttcggttcta aggatcgaaa gttggcttgt
     3961 taaaattctc aacttctatt catttaaata aagaacaaac cataatatga gtggagtata
     4021 tcggatttga accgatgacc tccagtgtgc gatactggtg ctctagccaa ctgagctaat
     4081 accccaaatg attgcagcaa aattacgtca gaattcttaa ttgggctcag aaatcctaca
     4141 taaaattcaa aacttatggt catacgcact gaaaaataaa tcccatacat tacaaatcaa
     4201 aaatttacaa ataaataaga tggttatttc cccatgatgc aattcgcaag atttaggttg
     4261 gaaaccattt ttacccaaat cctataccca ttcgaataaa ataaaatcta aatagttcca
     4321 ggtatttcat gataagcctt tatggtttga gtcaccatat tttcgacact gaaatttttc
     4381 accgtgatgt aagcattttc tttgagcata tttcgcaatt cagcattgca agccagacgt
     4441 aatacattag aagccagctg ttgtatatca ccaacaggtg ccagtaatcc attcatttga
     4501 tgggtaatca tttcggggat gccacctgcc gatgtagcta ctacattcac cccacatgcc
     4561 atagcgtcca gaatggttgt tcccagcccc tcgttttcgg atgggaagag caaaatatcc
     4621 agagaaggca aaatctcagg tatatcgtcc ctaaatccta acagaataac atttttctcc
     4681 aatcccaagt tttggatcat tttttcgatg ttcgctcgca taggaccatc gccagcaatc
     4741 acaaatataa aattctgccg atgtttcaat aattctgccg cagtttttac aaaaaccgga
     4801 tatcctttag gcttcgttaa agcggcaata ttaccaacaa taagataatc gtcgggaatg
     4861 tctaattcat tgcgtaattt gggataaggg ctccgataga gaaaacgctg taaatcgatg
     4921 ccactataaa tggtcagtaa tttatttctg tctttcacca ccgattccat aatcgttttc
     4981 acataattac tgacgcagaa aatcttggca atttcgggct gattatattt tttacgtgag
     5041 aagatatttt ttcctcgacg aatatcgact ctacggctca cgatcaaagg taaagccagt
     5101 tcttttcttc tggccaataa ataggcaagt gtatgagctc cactatcgtg aacgtgaatg
     5161 agatgaggcc gaacttcact gataaattgc cataactgga atggaacctt caagaagaag
     5221 ctaaatttcg atgaagaatc gaaagttttt atcgtaattt cttctttttt tgcttctttt
     5281 ctcaaagcgg cttcttcagg gcaaaacaag taatgcctaa cttctttgcc caatctgacg
     5341 agatacatga tttgttgttc acctccacgc caacctgttt gcgtcgaaac atgcaaaacc
     5401 tttatgggtt gtttgtccgc cataattcac gaagtttagc ataacgaata aagattccaa
     5461 atgccgataa tcgagcaatt tgatagcctt cataaccatc cataaacccg agttgaaaga
     5521 aataatgttt gaaaaattta tgtaagggtt tgagatagaa atgccaaaca ttgggtttga
     5581 ttcgtttttc aaaaagctca taagcccgaa tttgcataaa acgatcaatt tgctgaagat
     5641 gatcttctaa agaataatag gaatagtgca gaatgtcgcc tggaatatgg cgaatatttt
     5701 tactttcaaa aagttcgagt ttgtcgtgtg gattggtgcc tgtccagtag gctttttttt
     5761 tcaaaaccat cctcagtttt ttatctggat accagccact atggcgaatc cattttccac
     5821 aatagttggt tagtcgattc atggtatatc catcggccga aggattaaat ttaatttcct
     5881 ttatactatt ccttagcttt tcagaaagag cttcgtcggc atcgagagaa agaacccaac
     5941 gatgtgtggc ttgggcaagt gcatagttct tttgttgtac ataaccttcg aactcatgct
     6001 tgatgacctt cgcgccgaat tcacctgcaa tttctctggt tcgatcggtc gaaaatgaat
     6061 ctacaacaac tatctcgtca gcaattccaa ctaccgaccg tatacagcgt tcgatattcc
     6121 tttcttcatt ataagtgatg atgacaaccg ataaggcttc cattcgtctt aacaatttct
     6181 gagacaaaca attgtaaaaa aatgaatcgt aaagaaaatt cttacaaata ttgcgataaa
     6241 tttacaactc acataaaaag tagttcattc atcaaaaata aatcttctgc caaacatctt
     6301 catttcgtta aattccgcat ctatacatga aactgatttt cgaatccatc atttttactc
     6361 aaatctgttt aaaaattgta tatttgcgcc ttcatcagat atttcaatcc cctattttga
     6421 aagggataag aattcaatca aaatcgatac attaggcaaa atatttcttt gcctcttata
     6481 atgaagtgaa catggtttat acgatccagt ttaatgaata aaatatttac aggagttcaa
     6541 tgaatcattc acaaaatatt ttccttaatt cttatttcag tacaacacaa attattttct
     6601 tcatacttgg gggtgcattc ttgcttacca gtcttttctt tcagctaaaa aataaattca
     6661 agatttctgt gatattttta ggattgggtg ctttctgcat cttttgtttt gcagcactgt
     6721 tagacccgtt cctgaatctc tgggatgaac gctttcatgc tcttgtaggc aaaaatctga
     6781 tgaaacatcc actaatgcct actttatatg atgatcccat agtgcaaatg gaatatgata
     6841 aatgggatag atatcatatt tggttgcata aacaaccttt gtttctgtgg caaattgccc
     6901 tgtgtttcaa attattcgga atttctgaat ttgctttaag attgcccagt gtaattttag
     6961 gaactatttt ggttcttatt acctaccgtt cgggcaaatt actcgtcaat cagagagtgg
     7021 gttatattgc ggccatattg acattatcta cactttatat cattgaatta atagctggta
     7081 gagaagagct cgatcataac gacgtttctt ttttagttta tatttcacta agtatatggt
     7141 ctcttattga atatcaattc acacataaaa aaatatggat ttaccttatt ggtttattct
     7201 cgggcatggc gattttatgt aaatggttgg tgggtcttgt ggtatatctt ggatggataa
     7261 tcttaagagg tgtagaaaaa aaatttaaag ttaccgaaaa caaagatatt ttactttcac
     7321 tactaataac tataattata tcagcaccct ggcaaattta tattttatta aaatttccta
     7381 atgaggcgat tgcttcctac aaatataatg ctctgcattt taagacggct cttgaaggac
     7441 atggagggga tttgttttat catttcaaaa tgatgacaag aatttatggt tcatggtcaa
     7501 aatatctgat tattcctgcc tttatcactt tttttataaa aagtaaagat aaaaaattat
     7561 tctattcatt atccagcatt gtaataatag tttatgtatt cttttcattg gctgcaacta
     7621 aaatgccatc atacactata atcgttgtaa tgataaattt tattgctttt gctacgctca
     7681 ttgattttat attgaattat attagccatt tcattaaaaa tcctttggtt aatcatttaa
     7741 tatttgctgc ctctattctc atgatattca ccttgaagtt tgatataaac acattgagat
     7801 aaaaacatac cctttggaaa ggaaacaatt attatacaaa aatgatgatc aacaacaaaa
     7861 aaatatttac ttccttgaaa ttacctccca atagcgtatt attcaatgtc gaaggcagat
     7921 attatgtcga tgccatgttc tatacgggtt tacctgccta taattttctg ccttcaaccg
     7981 atcaatgcaa tgaccttaca aaaaaaggaa agcaaattgt aatactcaat tctaacaata
     8041 aagaaatacc agcttattat aaaaacgatc caaccattat gttcataaag gaggaactca
     8101 tttgtcatga ataaaatcaa caaaattaat ataaataata aacaggtcat cgtgtcggtc
     8161 actaaaaata aaaattttgt tacatataat ttatattttt tgttatttta ttataataaa
     8221 cataaaattt tgaaattagt taattttcat ttctaatgag atttgcattt gaataaattt
     8281 tttattttca gtttgacatg gttatttttc atttttctat ataaataaag tacataataa
     8341 ctaatttgat tttcatcatg aacttaagag acattatcag agaaatgctg attttccttc
     8401 acatcgattt aacaaagaac cttaaatatg ataggattac aaaacggata attaaaaatt
     8461 cgctaaaata taattctaat tgcattgaca taggctgcca taaggggcaa attttagatg
     8521 tgatgatcca atatgcacca gccggcaagc attatgcctt tgaacccatt ccttacctgt
     8581 tcgatgaact gcaaataaaa tataaaaaca aagcagttgt ttatcctttt gcattgtccg
     8641 ataccagtgg caaatcgtct ttccagcttg taaagaatgc ccctgcatat agcggaatca
     8701 aaaaaagaca ttacgatatt agtaatccgg atattgaaga aattaccgtc aaattaaaaa
     8761 cactagatga aattattccc gagaacgaaa tcattgattt tattaagata gatgtggaag
     8821 gagcggaatt ttgcgtattg aagggaggga aaaatttact gaaaaaaaat aagcctatta
     8881 tattatttga atttggaaaa ggcgccagcg attattatgg cacaaaacct tccgatttat
     8941 ttagttactt gaccgagcaa atcggtttga aaatttttac tctcgatgca taccttaaaa
     9001 gcaaacgaaa tataagtcag tcggaattcg aacatttata catttctgga aaggaatatt
     9061 attttatagc ggcatctgat attaaagaat aaaccttcgt gagaagcaaa atgctaatag
     9121 caataaaata ttattcactg aataaaaagt cacagaattt tttaaaaaat taatatataa
     9181 acaactataa acaaacaata tagaatctct taataatcat gggattttat taatactgtc
     9241 ttagcgagga agtattaaaa tggaaattct tcgggtattt cttcttgaat tttagtgctt
     9301 ttttctggag ctaaagcttg aaaatgtatg gggttggcag ccacatatat ggctttattg
     9361 ggcaaagcgg gacaggcata ttcgcatgca ccacaaccaa cgcagatatt ctgatttact
     9421 tcggga
//
LOCUS       DAOORU010000096         9393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_9084_length_9393_cov_5.227029, whole genome shotgun sequence.
ACCESSION   DAOORU010000096 DAOORU010000000
VERSION     DAOORU010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9393
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9084_length_9393_cov_5.227029"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="PLF20_06950"
     CDS             complement(<1..409)
                     /locus_tag="PLF20_06950"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOX80771.1"
                     /translation="MTTKTETKQFQYEIQAYQEDCVNNIISLFESLRQKANFKEVLTA
                     HHKKNKYNFPVQDTKNIDIMMETGTGKTFTFIKTIFELSKHFGYKKFIILIPTVPIRE
                     GTKTNLEDTKDYFKSLYANEKEKEIETFVYEGGN"
     gene            complement(455..1408)
                     /locus_tag="PLF20_06955"
     CDS             complement(455..1408)
                     /locus_tag="PLF20_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_091910270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOX80772.1"
                     /translation="MNILDFAINYPDEETCREKFKQQRDQMGVTCRRCNCKEHYWLEN
                     KQAYECKRCQARQTLRSGTVMQHSNLPYRYWFVAMHLLTATKGSFSAAEIQRQLGHKR
                     YQPIWEMVNKLRDVMGKRDDKYTLEGAIELDDAFFSTEISVEEKEKPLKRGRGSQKKT
                     KVLVMAESKTVENPKPGKKPKKAGYLKMKVINDLKADTITKNVKEHVESTADLTTDDS
                     TSYTKLKEHVHSHTTSVVPHEELPNVLPWVHTAISNAKRQLLGVYYKVKPEYLQYYLN
                     QFCYKFNRRYFGENQFDRLLIAAVSCAPDFKSRIYNRNYCG"
     gene            1865..2074
                     /locus_tag="PLF20_06960"
     CDS             1865..2074
                     /locus_tag="PLF20_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80773.1"
                     /translation="MSCRNTLAANSLFAQQDPPLPPGDASGNDNKAPRNGAPLGSGIG
                     LLLAMGAAYGGKKVYDYRKHKKEDN"
     gene            complement(2215..3555)
                     /locus_tag="PLF20_06965"
     CDS             complement(2215..3555)
                     /locus_tag="PLF20_06965"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOX80774.1"
                     /translation="MAKHSLIRDISGVMGSNILAMVSTLGISILLSRVLGPQGYGLFT
                     SLYVVPQVVMGLVQLGMSRSAVFHLGHKKFNECQTISSIYVMLCMTSTVGIIVSAIAY
                     AILNNPLLETNWILLALCIIPFALGNIYTSGIFIGKEDIQTSNKIYWIPVTLNLILTA
                     ITVGILQWGITGALIANLLATATIFFVFLLRIHHQYALSFRFDTKILKSLASKGLGFA
                     LAFVMLQLNYKVDILLLQKLAKPSEIGYYALGVSVTEQLWLLPYAIGVVLMSRTANAT
                     NERMMAKTTALMVRICLPAALIAALVLIALTPPLLPLIFGKSFIPSIRVVQTILPGIV
                     IFMIFRIIESYFAGLGKPMLSVMTLIPSLLLNIILNLLWIPRYGIIGSAMATNFSYAF
                     ATIIFLFVFQKYSRLSFKELLILKPSDLQRLKKGGRENGGKEMRRREEEGTIRV"
     gene            3637..4806
                     /gene="rffA"
                     /locus_tag="PLF20_06970"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             3637..4806
                     /gene="rffA"
                     /locus_tag="PLF20_06970"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR02379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HOX80775.1"
                     /translation="MHIPFNKPYFGPREAFATLGVALSGQFSGNGKYTQRCQTFFEKR
                     FHFAKTLLTTSCSDALEMAAILSEVGPGDEVIMPSFTFPSTANAFLLRGAKIVFADTL
                     PDIPNIDPKAIENLITPKTRAIVVVHYCGISCDMEAIQQLANKYGLLLIEDAAHSIDS
                     YYKGKPLGSFGHLAAFSFHETKNIISGEGGLLVVNDEQFTKRAEIIWEKGTNRAAFQR
                     KEVNRYEWVDIGSSFLPAEMVAAFLYAQLSKFDRIQKLRKKVWLSYYEYLKPLEEKGF
                     LQLPIVPEYASVNGNFFYIAVKDGYTRDELLRYLQSRGIHAVFHYLPLHQSPFFTFRH
                     DGRALPHTEKFSSTIIRLPFYPRLSKRKIKFVCNAISSFFESPQAYPVMVDHQMK"
     gene            5039..5659
                     /locus_tag="PLF20_06975"
     CDS             5039..5659
                     /locus_tag="PLF20_06975"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOX80776.1"
                     /translation="MLRDNIINTSVKLFRSMGIRSVTMEMVADEIGISKRTLYEHFKT
                     KDELVETCVHSELNRLIGKDTSIIERSSHIIETYLLFMWSHIQELRQTNYLFIKDLRQ
                     LYPSSLYCMTTEFESRIKEGIKNFIEKGKGQNIFRPEINVELATIILFEQLQMLMENS
                     EKIFSMHYFSPAEVFEHISINFIRGMATKEGVELIDHYYNEHQLTN"
     gene            5713..7050
                     /locus_tag="PLF20_06980"
     CDS             5713..7050
                     /locus_tag="PLF20_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX80777.1"
                     /translation="MKVNLKMILWGSLFIGFFLLCSIELKSQNDTLRLTLQQAVDRAI
                     EQNLLQQVSSLEIERKQAKVQEYMAALYPTIQGSGSYTRNIKKPVIFMPPESPFGPTL
                     KIGSDNAYSGSLTFSLPLFAMNVYESIKLGKMDAELSVEKERENRINLTANVRNTYYN
                     VLLLQQTVQVYNRSYQNAIENLKNIQQMQANGLVSEYDAIRAKVQVDNLYPNLLQAEN
                     IYQNLLNIFKIVLNIDTDTPLVLDETELLKVQLEELDTIPSTWYHDNPTLRQFGINRR
                     LLGIQKQMIRNSNIPILAAFGSYTYQTQANDFKFGDYNWVASSAIGLQLSVPIFKGFS
                     MRRQLSQAEIGLRQLDLQETYTRNNLEAQIQNAKNAIFAAIKKVNAAQGNVELAQKGY
                     QIARTRYNTGQATLVELNDAENAMMQAKLNLIQARSEYLTARNEYQKIIGKTL"
     gene            7096..8139
                     /locus_tag="PLF20_06985"
     CDS             7096..8139
                     /locus_tag="PLF20_06985"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX80778.1"
                     /translation="MKKKNFMAIMLLISVTFLVSCSNHKKESDATMQQTPNVVTAPIE
                     FTNIERMIKYPVSILAYKENHLAPATAGRVEKILVDVGDRVRAGQVVAQLDRTNYQQA
                     YIQYQKIRLDLMRMDSLLKVGAIAPQQYDQVKMQYDIAKTNLEFLEDNTTLKSPINGE
                     VTGKYLNDGEIFTMSPAAGVGKPAIVSIMQLDRLKLLVGVSSKYLPNIKRGMKVDVTS
                     EIYPGKIFSGSIDKIYPIVDNMSKTFTVEVIINNEGRILRPGIFATASIDIGKDQVLV
                     IPSFVIQKQSGTNERFVFIYKDGKAIRKIIEVGDVLDDKVEIVSGLNQGDELIVEGQM
                     VIKDGQAVNRISR"
     gene            8179..>9393
                     /locus_tag="PLF20_06990"
     CDS             8179..>9393
                     /locus_tag="PLF20_06990"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOX80779.1"
                     /translation="MSLYSESVRKPITTIMIFVAVIVLGIFSLINLPIDLMPDMDIPA
                     MSVITYYPGASAEDIEVNVTKPLENQLNSLQNLKKVISTSKDNISFILIQFEYGTNMD
                     QASNDVRDLLSLIEKTLPDQAEKPTLFKFNSSMMPVVIYGVTAQESYPALEKLIDERI
                     TERLSRIDGVGAVTVMGKPERIIDVRIDPRKLDSYGLTLEQIGNVIGAENLALPAGRM
                     EVGQMEFPVRTNGEFKSSDVVKDIVVFRSNAGLVYLKDIAQVKDTLKYIYVDERIDGE
                     VGVRLMVQKQSGANTVQIAKLVRERMDELSKELPKDVKIVKIVDTSEFIQNSINNLSE
                     TLLYAFIFVTLVVFIFLGRWRSTFIILLTIPVSLIAAFIFLYAIGGTLNIISLSALAI
                     AIGMVVDDAIVVL"
BASE COUNT         2881 a         1837 c         1957 g         2718 t
ORIGIN      
        1 tatttccacc ttcgtaaaca aaggtttcaa tttccttttc tttttcgttg gcgtataagc
       61 ttttaaaata atctttggta tcctctaggt ttgtttttgt tccctcgcga ataggaacag
      121 tagggataag aatgataaat tttttgtagc cgaaatgctt actcagttca aaaatcgttt
      181 tgataaacgt gaacgttttc cccgttcccg tttccatcat aatgtcaata tttttagtgt
      241 cttgaactgg aaagttatat ttattctttt tgtggtgtgc tgtcaacacc tctttaaagt
      301 ttgctttctg gcgaagactt tcaaaaagac tgataatatt atttacacaa tcctcttgat
      361 aggcttgtat ttcgtattga aattgttttg tttctgtttt tgtcgtcatt tattacttca
      421 attttccaat tgtcaaagtt aaaattttaa atgattatcc gcaatagttc ctattgtaaa
      481 ttcttgattt gaaatcaggc gcacacgata cggcggctat caacagcctg tcaaactggt
      541 tctcgccgaa gtaacgcctg ttgaacttgt aacagaactg attgaggtag tactgtaaat
      601 actcgggttt taccttgtaa tacacgccca ggagctgtcg tttggcattg ctgatcgcgg
      661 tgtgtaccca aggcagcacg ttaggcaatt cctcgtgtgg aacaacggat gtcgtgtgtg
      721 aatggacatg ttctttcaat ttagtgtaag aagttgaatc atcggtggtc agatccgccg
      781 tgctttcgac atgctctttg acattctttg taattgtatc ggctttcaag tcgttgatga
      841 ctttcatctt caggtatccg gccttcttgg gttttttacc cggtttgggg ttttcaaccg
      901 ttttgctttc agccatcacc aggaccttgg tctttttttg gcttccacgg ccgcgtttca
      961 acggtttctc cttctcttca acggatatct cggtggaaaa gaaggcgtcg tccagctcga
     1021 tggcgccctc gagcgtgtac ttgtcgtcac gcttgcccat cacgtcacgc agtttattga
     1081 ccatttccca tatgggctgg taacgcttgt gccccagctg tcgctgtatc tcggcggcgg
     1141 aaaaggagcc cttggtagcc gtgagcaggt gcatggccac gaaccagtaa cggtatggca
     1201 ggttggaatg ctgcatgacc gtgcccgaac gcagcgtctg gcgcgcttgg caacgcttgc
     1261 attcgtaagc ctgcttgttt tccagccaat aatgctcctt acaattacaa cgacggcagg
     1321 taactcccat ttggtctctt tgttgtttga atttttcccg acaggtttcc tcatcggggt
     1381 aatttatagc aaaatccagg atattcatag cttgaaatat tacatgtgtt cagaatgtaa
     1441 atataatgaa tatctgtgat ttgtgtaagc atttgcgaac ttatttataa ctatttttcc
     1501 gctaaattct ttaggaataa ctgcggatat acattaaatt taatattgtt gtctgcccgt
     1561 ttttctcttg aaatccactc gtattgtgtt agggtggtgg gtgggcttgg gtgcgtaggc
     1621 aagagcaagc tcttttgcaa gctgaagcat cggcttgtgc gttggcaaaa atgcaaatgt
     1681 gcttgctgtg cggtggctgt ccttcaaaaa tggttttaaa aaatatgcgg tggaaaaaaa
     1741 ataaaaaaca ttgggtcggc ttgagtgtcg gctcaaatgt cctgttgaag tatggtcttt
     1801 atgttgtctg tcacctgtca aaatggttta ctttttcttc tctgtttaac ggtcattttc
     1861 tgttgtgtcg tgtcgtaata cgcttgcagc taacagttta tttgcgcaac aagacccacc
     1921 actaccgcca ggtgatgcaa gtggtaatga taataaagca cctcgtaatg gagctccttt
     1981 aggcagtggc ataggtttac tattagccat gggtgctgct tatggtggca agaaggtata
     2041 tgattaccgc aagcataaga aggaagataa ttaaacctga gttaaggagg tggatagagc
     2101 catgttttct cactaaagag gaggcatggc ttttttagta aaaagggaat ggagtttggg
     2161 agttaaaaat tgaaaggctg aaaatattct atcatttaag aaaaaattca taaattaaac
     2221 acggatagtt ccctcctcct ctcgtcttct catctccttt cctccgtttt cccttcctcc
     2281 ctttttaagc cgttgcaaat cggagggttt cagaataagg agttctttga acgaaagtct
     2341 gctatatttc tggaatacaa aaagaaatat aattgtagcg aaagcataac taaaattggt
     2401 agccattgcc gagccaataa tgccatatcg aggtatccag agcaagttga ggataatatt
     2461 caacagaagg gaaggtatca gcgtcatgac gctaagcatg ggttttccca aacccgcgaa
     2521 atagctttcg atgattctga agatcatgaa gatgacaata cctggcaaga tggtttgaac
     2581 gacacgaata ctgggaatga aggattttcc aaaaatcaag ggcagcaaag gaggagtaag
     2641 ggcaatgaga accaaagcgg caatcaatgc tgcaggcaga caaatacgca ccatcagggc
     2701 agtggttttg gccatcattc tttcattggt cgcattagcc gtccgactca tcagaacaac
     2761 tccaatggca taaggcaata gccataattg ttcggtaacg gaaacgccaa gagcataata
     2821 accaatttca gaaggtttgg ctaatttctg gagcaaaaga atatccactt tataattcaa
     2881 ttgaagcata acgaaggcca aagcaaaacc caaaccctta ctggcgaggc ttttgagaat
     2941 tttggtatcg aaacggaaac taagggcata ttgatggtga atgcgcaaga ggaacacgaa
     3001 aaatatagtg gctgtggcta ataaattggc tataagtgct cctgtgatgc cccattgaag
     3061 aatgccaacc gtaatggcag tcaaaattag attgagagta accggtatcc agtaaatctt
     3121 attcgatgtt tgaatatctt ccttgccaat aaatattcca gatgtataga tgttacccag
     3181 tgcgaatgga ataatgcaca aagccagtaa aatccaattg gtttcgagca atggattatt
     3241 caatatggca taagcaatag ccgatacgat gatcccaaca gtagaagtca tacacaacat
     3301 cacatagatg gaagaaatgg tttggcattc gttaaatttt ttatggccta gatggaatac
     3361 agccgaacgg ctcattccca attgcaccaa acccattacc acttgaggca caacatataa
     3421 ggaagtaaag aggccatatc cttgggggcc aagaactcgt gaaagcagaa tgcttatgcc
     3481 cagagtactg accatggcca gaatattact gcccattact ccgctaatat ctcttataag
     3541 gctatgtttt gccataaatt catatgcgaa ggtaaggatt tcgacccgag cacgagaata
     3601 tgtaatttcg cagcctaaac cacaaattaa acaaaggtgc atattccttt caacaaaccc
     3661 tattttggcc cgcgagaagc gtttgctact cttggtgtgg cactaagtgg tcaattttcg
     3721 ggtaatggga aatacaccca aaggtgtcag acattttttg aaaagaggtt tcattttgca
     3781 aagactttac ttaccacttc atgcagcgat gctttggaaa tggcagccat tctttcggaa
     3841 gttgggccag gcgatgaagt catcatgcct tcgtttacat ttccttccac cgccaatgcc
     3901 ttcctactga ggggagccaa aatcgtcttt gccgatacct tacctgatat tcccaacatc
     3961 gatccaaaag ccatagaaaa cctcattacg ccaaaaacac gcgcaattgt ggtggttcat
     4021 tattgcggca tatcctgcga catggaggcc attcagcaat tggcaaacaa gtatggcctt
     4081 ttattaatag aagatgcagc tcactccatc gattcctatt ataaaggaaa acccttgggg
     4141 agctttggcc atctagcggc cttctcattt cacgaaacaa aaaacatcat ctcgggagaa
     4201 ggtggcttat tggtcgtaaa cgatgagcaa tttacgaaac gagccgaaat tatatgggaa
     4261 aaaggtacca acagagctgc tttccagcgt aaagaagtca atagatacga atgggtagat
     4321 attggttctt cttttttgcc agccgaaatg gtagccgctt ttctttatgc acagttaagc
     4381 aaattcgatc gcattcagaa gcttcgtaag aaagtatggc tttcatatta tgaatacctt
     4441 aagcctttag aagaaaaagg atttttacaa ctccccatcg tgcctgaata tgccagcgta
     4501 aatggaaatt tcttctatat cgcggtgaaa gacggttaca ctcgagacga attgcttaga
     4561 tatctgcaaa gcagaggcat acatgccgtt ttccattatt taccccttca tcagagccca
     4621 ttttttactt tccgacacga cggacgagct ctgccgcata ccgaaaaatt ttcgtccaca
     4681 atcattcgtc ttccctttta tccaaggctc tcgaaaagaa agatcaaatt cgtttgcaat
     4741 gctatttctt ccttcttcga aagcccgcaa gcatatcctg tcatggtaga tcatcagatg
     4801 aaatgaaaaa aaattcatgg aatcatccat ctgttatccc tcattcatga attcttgaat
     4861 aaaagattga ttttgagtta aaaaccaaaa aatattctct aacataaaaa ttggacggat
     4921 attggaatat gttaaatttt cttgaaattt tatttttacg agcaatttgt aagaatattt
     4981 gttatacatt tgcacgtcga aaacttaaaa agttttttaa gttttcaaat gggtaaaaat
     5041 gttaagagac aatatcatca atacttcagt caagcttttc aggagcatgg gtatacggag
     5101 cgtaaccatg gaaatggttg ctgatgagat cggtatatcg aaacgtactt tatatgaaca
     5161 tttcaagacg aaggacgagt tggtggagac ctgtgtacat tcagaactta atcggctgat
     5221 aggaaaagac acttctatta ttgaaaggag ttctcacatt atagaaacct accttttgtt
     5281 tatgtggagt catattcagg aattacgtca aaccaattat ctatttataa aggacttgag
     5341 gcagctttat ccgagcagcc tctattgcat gactactgaa tttgaatcga gaatcaagga
     5401 agggataaaa aatttcattg aaaaggggaa agggcagaac atcttcaggc ctgaaataaa
     5461 tgtagaatta gcaacgatta ttttattcga acaattgcaa atgttaatgg aaaatagtga
     5521 aaaaatattt tcgatgcatt acttttcacc cgccgaagta ttcgagcaca tttccatcaa
     5581 tttcatacgg gggatggcta ccaaagaagg agtcgaatta attgatcatt attacaatga
     5641 gcaccagcta accaattaaa ccatttcaaa agagattatc caatatcaga atataaacaa
     5701 tagacacgag atatgaaagt aaacttaaaa atgattcttt ggggaagcct gtttatcggt
     5761 ttctttttac tttgcagcat agaattaaag agccaaaacg ataccttgag gttaacgctt
     5821 caacaagctg ttgacagggc tatcgagcaa aacctcttac agcaggtttc ctcactcgag
     5881 atcgagagaa aacaagccaa ggttcaggag tatatggcag ccttgtatcc aacgattcag
     5941 ggcagtggaa gttatacgcg taacatcaag aaacccgtta tcttcatgcc gcccgaatcg
     6001 ccttttggcc ctactcttaa aataggatcg gataatgcct actcaggttc gcttaccttt
     6061 tcacttcctt tgtttgcgat gaatgtttat gaatctatca aattaggaaa gatggatgcc
     6121 gaattaagcg tagaaaaaga aagggaaaac cgaatcaacc tcaccgccaa tgttagaaat
     6181 acttattata atgtattgtt actgcaacaa acggttcagg tgtataatag aagttatcag
     6241 aacgccatcg aaaacctaaa aaatattcag caaatgcagg ctaatggatt ggtatcggaa
     6301 tacgatgcta tcagagcgaa ggttcaggta gataatcttt atcccaatct tttacaggct
     6361 gaaaatatct atcagaatct cctcaacata tttaaaatag tattgaatat cgataccgat
     6421 actcctttgg ttttggatga gacagaatta ctaaaggtgc aactggaaga actcgacacc
     6481 attccttcta catggtatca tgataatccg actttgaggc agtttggaat caatcgtcga
     6541 ctcttgggca ttcagaagca aatgattcgc aatagcaata ttcccatatt ggcagcattt
     6601 ggcagctata cttatcaaac acaggccaac gatttcaaat ttggcgatta caattgggtg
     6661 gcttcttctg caataggttt acagctttcc gttccgattt tcaaaggatt ttcgatgcgc
     6721 cgtcaactga gccaagccga aataggtttg cgtcagctcg atcttcagga aacctataca
     6781 cgcaataatc ttgaggctca gattcaaaat gctaagaatg ctatttttgc tgccattaaa
     6841 aaggtgaatg cagcccaagg caatgttgaa cttgctcaga agggatatca gattgcccgt
     6901 acacgataca atactggaca ggccacattg gtcgaactga atgatgcaga gaacgccatg
     6961 atgcaagcca agctcaatct catccaggcc agatcggaat atctaactgc cagaaatgaa
     7021 taccaaaaga ttatcggcaa aacattgtaa ttgcaatata aaataattat acaagaataa
     7081 aacatacaac caactatgaa aaagaaaaac ttcatggcaa taatgctgct tataagcgta
     7141 acctttctgg tttcatgcag caaccacaaa aaagaatccg atgctacaat gcaacaaacc
     7201 ccaaatgtag taacggcccc tattgaattc accaatatcg agcggatgat caaatatccg
     7261 gtctccattc ttgcctataa ggaaaatcat cttgctccgg caactgcggg gagagtagaa
     7321 aaaatcctcg tcgatgtggg tgatcgtgta agagccggtc aggtggttgc tcagctcgac
     7381 cgcaccaatt accagcaagc ctatattcaa tatcaaaaaa tacgtttgga tttgatgcgc
     7441 atggattctc tgttgaaagt cggagcaatc gccccacagc aatacgatca ggtaaagatg
     7501 caatatgata tagccaagac caatcttgaa tttctcgaag acaacaccac cttgaaatcg
     7561 cccatcaatg gagaagttac gggaaaatac ctcaatgatg gtgagatctt tactatgagc
     7621 cctgcagcag gcgttggcaa acctgctatt gtgtctatca tgcaactgga tcgtttaaaa
     7681 ttgctagtgg gcgtttcatc aaaatatctt cccaatatca aaagaggcat gaaggtggat
     7741 gttacttcag aaatttatcc tggaaaaatt ttctcgggaa gcatcgacaa gatttaccct
     7801 atcgtagata atatgagtaa aactttcact gtagaggtaa ttatcaataa tgagggtcgt
     7861 atattaaggc caggcatatt tgctactgcc tccatcgata ttggtaagga ccaggttttg
     7921 gtcattcctt cttttgtaat tcagaaacaa tcgggaacca acgaacgctt cgtatttata
     7981 tataaagatg ggaaagccat acgcaaaatc atagaagttg gagatgtttt ggacgataag
     8041 gtcgaaatag tgagcggctt gaatcaaggc gatgaattaa ttgtcgaagg acaaatggtt
     8101 attaaggatg gacaggctgt aaatcgtata tcaaggtaaa ccctgtcgga aacttaaaat
     8161 ttttaaaatc gaaaatatat gagtttatat agtgaatcag taagaaagcc cattacaacc
     8221 ataatgattt ttgtggctgt gattgtgttg ggaatatttt cgttaataaa tttacccatt
     8281 gatttaatgc ccgacatgga tattccggcg atgagtgtga ttacttatta cccgggtgcc
     8341 agtgcggagg atatagaggt gaatgtaacc aagcctcttg aaaatcaatt aaactcttta
     8401 cagaatttaa agaaggtcat ttccaccagc aaggataata tctcattcat tcttatccag
     8461 tttgagtatg gaaccaatat ggatcaggca agtaatgatg taagagattt acttagtctt
     8521 atcgaaaaaa ccttgcccga tcaagccgaa aaacccacct tattcaagtt caattcttcc
     8581 atgatgccgg ttgtaattta tggagtaaca gctcaagaaa gttatccggc gctcgaaaag
     8641 ctcatcgacg aacgtatcac agaaaggctt agccgtattg atggagtagg tgcagtcacg
     8701 gtaatgggca aacctgaacg tattattgat gtgagaatag acccacgcaa gctggattca
     8761 tacggcctaa ctctggaaca gatcggaaac gtcattggag ccgaaaacct tgcactccca
     8821 gccggtagaa tggaagtcgg gcaaatggaa ttccccgtaa gaaccaatgg agaatttaaa
     8881 agcagcgatg tagtaaaaga cattgttgtt ttccgatcga atgcaggttt agtatatttg
     8941 aaagacatag ctcaagtaaa ggatacccta aaatacattt atgtagatga acgcatcgac
     9001 ggggaggtgg gtgtaagatt gatggtacag aaacagagtg gagccaatac tgtgcaaatt
     9061 gccaaattag tacgcgaaag aatggatgaa ctcagtaagg aactccctaa ggatgttaaa
     9121 atagttaaaa tcgtcgatac atctgaattc atccaaaatt ctatcaataa tctatcggaa
     9181 accctattat atgcctttat atttgttact ctggtagtat tcattttttt aggcagatgg
     9241 cgttcaacct ttatcatctt gcttaccatc cctgtctcac taatcgctgc ctttatcttc
     9301 ctttacgcta ttggtgggac tttgaatatc atatcgcttt cggcccttgc cattgcgatt
     9361 ggtatggtag tggacgatgc cattgtggta ttg
//
LOCUS       DAOORU010000097         9370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_9111_length_9370_cov_5.703596, whole genome shotgun sequence.
ACCESSION   DAOORU010000097 DAOORU010000000
VERSION     DAOORU010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9111_length_9370_cov_5.703596"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLF20_06995"
     CDS             <1..253
                     /locus_tag="PLF20_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80780.1"
                     /translation="KFILLLKILMTVWTDSNYMKRRKGSKEVFDGYFKFEGRYIWYYG
                     YKTKDNDTLHLYLEEKLHVEEEKDFLERIENGSKVYTIE"
     gene            877..2181
                     /locus_tag="PLF20_07000"
     CDS             877..2181
                     /locus_tag="PLF20_07000"
                     /inference="COORDINATES: protein motif:HMM:NF028426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HOX80781.1"
                     /translation="MKNAFASILLIIIVFSSILAQDDIAFYSQKALRTQNRIYNPDIK
                     TVLIFPTGYPLEMPVISLNSDKTLQLQFDDLAGGVKNFQYTFLHCDANWEPSQLRMNE
                     YMEGFDSDEIRDYKFSFNTTTSYTHYSLIFPNDRIRLTKSGNYLLVVYLDSPTQPEFS
                     LRFIIYEPRVIIQDVKIGRAHLPAYMNTKHEVDFTIRPVKYKIPVPDRDLTIVILQNW
                     RWDNALTIKQPRNITPDLLDYDYEEENLFDAGNQYRSVDIKSLRYRSEYIADILYLAD
                     GYHVVMQLDRIKAGKPFVNDPDLNGRLYIKTEDMEHSETEADYAMVHFSLPLGYPLAH
                     ASIFIMGELTGNSLQPEAKMIYNYETMRYEGSLRLKQGYYNYIYVVANHESGAGDTSI
                     IEGNFWETDNEYTILVYHREPGEIFDKLVGYYQSGNEYSPKQ"
     gene            2234..3025
                     /locus_tag="PLF20_07005"
     CDS             2234..3025
                     /locus_tag="PLF20_07005"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOX80782.1"
                     /translation="MEKTENEVIIIGAGNVGTHLAHQLDKKNFTIRGICSRTIQSARQ
                     LAKYYVCESWTNAALIPPDAQYYFICVNDEAIAKVADAMPQTNGLVMHTSGSTPIEVL
                     SRFPNHGVFYPIQTFTKNHETDFSLIPICIEASNKKNLNILKKLALQFSNNIIEANSA
                     QRKHIHLSAVFACNFTNYMYVIAHDILNEQKLPFSILLPLILESFEKVTLMEPWQAQT
                     GPAVRGDSSIIEEHIRMLSENPDYEVIYRIISEQITKRKNKEKTE"
     gene            3061..3540
                     /locus_tag="PLF20_07010"
     CDS             3061..3540
                     /locus_tag="PLF20_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005936823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HOX80783.1"
                     /translation="MFDYDGVLTTGEIFILPDGEVLRGGYVRDGYAIQHAIKNGYRIV
                     IISGGKSDSMVHRLNALKVNDFFLGIADKLSIFNEYIHLHHIEPQGVLYMGDDIPDLT
                     VMQQVGLPCCPADASEEIKAISLYISHREGGKGCVRDVIEQVMKLQGKWMNGDAFYW"
     gene            3518..4537
                     /locus_tag="PLF20_07015"
     CDS             3518..4537
                     /locus_tag="PLF20_07015"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HOX80784.1"
                     /translation="METHFTGKPDIVMGKSLLCRIKTQFTVYARLLRWINLLMILLIV
                     LIIRYGFFLPLYMAIGLASPLSHTIYFLVVLSIVLITAAGYIVNDIYDQKIDRLNKPT
                     RLVIGQAVSVSRGWILYVALNIFGLISGGIAALQIEQPMLLWLFVLSFGLLWYYSAYL
                     KRKPFWGNFLVSFLSALVIITPWLFEFYALLLKPDEWIAFYRFYPHVNYIILSLSIFA
                     FISTLIREIIKDAEDMEGDQTYECQTLPIILGWKTTHRILQALIALMMILLAMAQYWL
                     RSHHYNLAFYWYFTVQFLSIRLLTVLPADVNKNQLHRCSSLAKITMLAGILGILALIP
                     PLFST"
     gene            4587..4991
                     /locus_tag="PLF20_07020"
     CDS             4587..4991
                     /locus_tag="PLF20_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOX80785.1"
                     /translation="MIEIFNKALISRFIKFIIVGFSGLIVDYGITAFSKEILKIQRFI
                     SNAMGFICAASSNYYLNRIWTFSSTNPEVVREFGFFFVISLIGLGINSLILWFVLKKF
                     KLNFYLAKIFAIAGATLWNFFANAFITFQMPI"
     gene            complement(5655..7748)
                     /locus_tag="PLF20_07025"
     CDS             complement(5655..7748)
                     /locus_tag="PLF20_07025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005778221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HOX80786.1"
                     /translation="MYKINRNLFISICLVMSLTACVRQEKKGTSDEIIGPKTLQLEND
                     LLTAELLWNIGRVSDIQLSPDASEILFRVTWYNLDKNKGNHELYLMNSDGNNCRRITH
                     TPFSEYEARWTPDGKITFISSESGTPQIWIMDKNGKNRKQLTRVENGVNGYKFSADMS
                     LLVYVSDVPRQRIKKKLFEGLDKTSGRLMNDLMYRHWDSWVDAFSHIFITTFNGQPVS
                     QGKDIMNGEPWESPIRPFGGMEQVAISPDSKTIIYTCRKKQGKEYALSTNTDLYAYSL
                     ATGQTTNLTSGMMGYDMEPVFSPDGKYLAWWSMERDGYEADQQRLFLLNLFTGEKIYA
                     SQGFDGDVSQITWSKHGDLIYFISNHQATYQIYSYSMADGEIRQVTDGMHDFKTVLDG
                     GDKLYGIRMSIQQPDEIFSIDPSNGEFTEISFINKDLFARLTLGKVEERWTKTTDGKM
                     MKSWVIFPPHFDSTKSYPTLLFCEGGPQNATSQFWSYRWNFQLMAANGYIIVAPNRRG
                     VPGFGQEWKEQVSGDYGGQNMKDYLSAIDDVTSEPWSDENHLGAIGASYGAFSVYWLA
                     GHHNKRFKAFIAHDGMFNLEAQYLETEEMWFVNWDLGGPYWDKDNAIAQRSFANSPHR
                     FVDKWDTPILIIHSEKDYRIVASQGMSAFNAAVLRGIPAEFLYFPDENHWVLQPQNSV
                     LWQRVFFDWLDQWLK"
     gene            7988..8593
                     /gene="pnuC"
                     /locus_tag="PLF20_07030"
     CDS             7988..8593
                     /gene="pnuC"
                     /locus_tag="PLF20_07030"
                     /inference="COORDINATES: protein motif:HMM:NF016837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HOX80787.1"
                     /translation="MESFWNLLIHNIQRTAPLEFIAVGFGLLSVFFIMKENIWGYPTG
                     LLNVGLYVYLCFKAGLFANMGINVFYFIVSIYGWYNWAHPGEQETRLRVKRLNRKETL
                     IAICSMMILLFFIWRLLRDFPESSYPFFDALTTSIFIIAMVLQALKCLESWILWIAGD
                     FIAIPLFASSGLAFTCLQYLAFLIMAISGFFSWKKSLLRND"
     gene            8586..9143
                     /locus_tag="PLF20_07035"
     CDS             8586..9143
                     /locus_tag="PLF20_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX80788.1"
                     /translation="MIKISITGPESTGKSWLSENLAYVFNTIWVKEYAREYLERLDHP
                     YTIDDVITIARKQRQLEHEIASYKPHLMFCDTDMLVCKIWCEVKFGYCPPQIIELYQE
                     DDYQLYLLMDIDLPWVYDPLREHPHFRQELFKIYLNTLTLDHKPFEVIKGLNGERLIN
                     AVNAIMHHFVGIKEMLIPERIQSYE"
     gene            9204..>9370
                     /locus_tag="PLF20_07040"
     CDS             9204..>9370
                     /locus_tag="PLF20_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005779953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOX80789.1"
                     /translation="MSVSDNLKKILSTLPQGVKLVAVTKTHPPEVIMEAYQAGHNIFG
                     ENKAQEMRSKY"
BASE COUNT         2817 a         1872 c         1807 g         2874 t
ORIGIN      
        1 gaagtttata cttttactga aaattttgat gactgtatgg acagactcta attatatgaa
       61 aaggagaaaa ggaagcaagg aagttttcga tggttatttc aagttcgaag gtcggtatat
      121 ttggtattac ggttacaaga caaaagacaa tgatacgctt cacctgtatc tggaagaaaa
      181 actacatgtc gaagaggaaa aggattttct ggagagaata gaaaatggct caaaagttta
      241 caccatagaa tgattcaaca aaaagaatcc agagtttggt acgattgctc tgcttacaaa
      301 tgctgaaaag acgccacaga agatttactc gaatataaaa gtcgtggaga ggcggagcaa
      361 atgattgaca ccttgaagga cgtagtagaa gccgatatga gttacatgca aaacgaattt
      421 gcactcgaag gctggatgtt tatcaatttc attgccttac actgatatta tagaatatat
      481 caattgttga caaaacatga actcaatgga aaatttttca cttttggctt gattaatttc
      541 ctaaccagaa taaaaacgaa tgaaaatcaa cgataaataa aataccatta aaattaccaa
      601 ggaaggagct aacctttcga aaaaattgga gctgcctaaa acgtaaattt tgcgaattag
      661 gtcttaaata aacttatttc caaaccatct atcgattccg gaagcataat tttcgatcta
      721 cttattcttg tggcaattca gaactctcga tttaagccga atcaaggggt ccggataata
      781 gaagtgaagt gagtaagtgt tggaacagag atttttttcg aataaattcg tcgtattcac
      841 tgctctttct tatcttcgca taataaaaat acaagtatga aaaacgcatt tgcttctatt
      901 ctattgataa tcattgtttt tagttcgata ttggctcagg atgatatagc gttttatagt
      961 caaaaggcat tacgaacgca aaacagaata tacaatcccg acattaaaac cgttttgatc
     1021 tttccaacgg gttacccctt agaaatgcct gtaatttcgc ttaattcgga caaaacgcta
     1081 caactgcaat tcgacgattt ggcaggtgga gtcaaaaatt ttcaatacac cttccttcat
     1141 tgcgatgcca attgggaacc atctcaacta aggatgaatg agtatatgga aggattcgac
     1201 tcggatgaga ttcgtgatta caaattttca ttcaacacca ctacctccta tacgcattac
     1261 tcgttaatat ttccgaacga tcgtatcagg ctcaccaaat caggaaatta tcttcttgta
     1321 gtttatctcg attcgcctac tcagccagag ttttccttac gattcataat ttatgaaccc
     1381 agagtaataa ttcaggatgt taaaattggc agagcacatt tacctgctta tatgaatacc
     1441 aaacatgaag ttgatttcac aattcggccg gtgaaataca agattcctgt accggacagg
     1501 gatctcacca ttgtgattct ccagaactgg cgttgggaca atgcgcttac aataaaacag
     1561 ccccgcaata ttacacccga cttgttggat tatgattatg aagaagagaa tttattcgat
     1621 gcaggcaatc aatatcgctc ggtcgatatt aaaagtttac gctatcgttc ggaatatatc
     1681 gcagacatcc tctatcttgc cgatggttac catgtagtga tgcagcttga tcgaatcaag
     1741 gcaggcaagc cgtttgtaaa cgatcccgac ctcaacggtc gtttatacat caaaacagaa
     1801 gacatggaac acagcgaaac tgaagccgat tacgccatgg tacatttttc acttccattg
     1861 ggatacccat tggcacatgc aagcattttc attatgggtg aacttacagg aaattccctc
     1921 cagccagaag ccaaaatgat ttataattac gaaactatgc gatatgaagg aagtttacga
     1981 ttgaaacaag ggtattataa ttatatttat gtcgtggcca atcatgaatc cggtgcaggt
     2041 gatacttcca tcatcgaagg aaacttctgg gaaaccgaca acgaatacac cattttggtc
     2101 tatcatcgcg aaccaggtga aatattcgac aaacttgtag gttattatca atcgggtaat
     2161 gaatacagtc caaagcaata agggaaggat gagatatttt taaagaatta cttttgtata
     2221 aattttttga agaatggaaa aaaccgagaa tgaggtgatt attattgggg caggcaacgt
     2281 agggactcat ctggcacacc aactcgacaa aaaaaatttt accatcaggg gaatatgcag
     2341 ccgtacgatt cagtcggcac ggcaactggc aaaatactat gtatgcgagt catggacgaa
     2401 tgcagcctta attcctcccg acgcacaata ttattttata tgcgtgaacg atgaagccat
     2461 tgcaaaggtg gcggatgcca tgccccaaac caacggcttg gttatgcata cttcaggctc
     2521 gacacctatc gaagtcctgt cacgtttccc taatcatggc gttttttatc ctatacaaac
     2581 ctttaccaaa aatcacgaaa ccgatttttc gctgatcccg atttgcatcg aggcctctaa
     2641 taaaaagaac ctcaatattc ttaaaaaatt agcccttcaa ttttcgaaca acattatcga
     2701 agccaattcg gctcaaagaa aacacattca tttatcagcc gtttttgctt gcaactttac
     2761 caattacatg tatgtgatag cacacgatat tctcaatgaa caaaaattgc ctttctctat
     2821 tcttttgcct cttatactcg aatcattcga aaaggttacg ttgatggaac catggcaggc
     2881 ccaaaccggc ccggctgtca gaggcgattc ttcaattatt gaagaacata taagaatgct
     2941 ttccgaaaat cccgattatg aggttattta tcgcattatc tctgaacaaa tcaccaaacg
     3001 aaaaaataaa gaaaaaacag agtgaattat aaagaattac ttcatcagat tactacgttc
     3061 atgttcgact atgatggtgt gcttaccacg ggagaaattt tcattctacc tgatggtgaa
     3121 gttctaagag gaggatatgt gcgtgatggt tatgccattc agcatgccat aaagaatggc
     3181 tatcggattg taataattag cggaggcaag agtgattcga tggttcatcg cctgaatgcc
     3241 ctaaaagtaa atgacttttt cttaggcatt gccgataaac tctccatctt caatgaatat
     3301 attcatcttc atcatattga acctcagggt gttttgtata tgggggatga tattcccgat
     3361 ctcaccgtaa tgcagcaggt tggcttacct tgctgccctg ccgatgcctc tgaagaaata
     3421 aaagcaatct ctctttacat ttctcacaga gaaggaggca aaggttgcgt aagagacgtg
     3481 atcgagcagg tgatgaagct tcaagggaaa tggatgaatg gagacgcatt ttactggtaa
     3541 gccagatatt gttatgggaa aatccttact ctgcagaatt aaaacccagt tcactgttta
     3601 tgcacgcctt ttacggtgga tcaacctatt gatgatattg ctcatcgtcc tcatcattcg
     3661 ttatggcttc ttcctgccgc tttatatggc tataggatta gcttcgcctc tcagccatac
     3721 aatatatttc ctcgtcgtac tgtcaattgt gctaatcacc gcagcaggct atatagtaaa
     3781 tgatatttac gaccagaaaa tagatcgcct gaacaaacct acaaggttag taataggaca
     3841 ggcggtgagt gtaagccgag gatggatcct atatgttgct ttgaatattt ttggtttgat
     3901 ttcaggggga atagcagctc tacagataga gcaacctatg ctcttatggt tgtttgtttt
     3961 atcgttcggt ctgctttggt attactcggc ctatctaaaa cgaaaaccct tctggggaaa
     4021 ttttctcgtg tctttcctct cggctttagt cattattaca ccttggcttt tcgaatttta
     4081 tgcactgttg cttaagcctg acgaatggat tgcgttttat cgtttctatc ctcatgtaaa
     4141 ttatattata ctgtcgttga gtattttcgc ttttattagc acattgataa gagaaattat
     4201 caaggacgcc gaagacatgg aaggcgatca aacttatgaa tgccaaaccc tgcccattat
     4261 tcttggatgg aaaactacgc acagaatact gcaggctctc attgctttga tgatgattct
     4321 gttagcaatg gcgcaatatt ggcttcgatc ccaccattat aaccttgcat tttactggta
     4381 tttcacggtt caattcttat cgatccgttt gcttacggtg ctccccgccg atgtcaacaa
     4441 aaaccagttg catcgctgtt catcccttgc taagattact atgctggcgg gaatacttgg
     4501 gatccttgca ctgatccctc ctttgttttc gacataacac actatctatg aataaattat
     4561 caaatctttg attaatccga ttaattatga tagagatatt caacaaagcc ttgatcagca
     4621 gattcataaa gtttataatc gtgggatttt caggacttat tgttgactat ggtattacag
     4681 cattttcgaa ggaaatttta aaaatacagc gcttcatatc taatgcaatg ggatttattt
     4741 gtgcagcctc atcgaactac tatctgaacc gaatctggac cttttcgagc actaacccag
     4801 aagttgttag agaattcggc ttctttttcg ttatttcatt gataggtttg ggtattaata
     4861 gccttattct atggtttgtc cttaaaaaat tcaaactgaa cttttacctt gcaaagatct
     4921 ttgctattgc gggagcaaca ctttggaatt ttttcgccaa tgcttttatt acctttcaga
     4981 tgccaatcta attaaccatc tcctgccctt ccaaaatgtt atattttctt ttacaacgct
     5041 gtcagatatg ttcatttcta tatattttac ccggctataa ctatttaaat aacacatttt
     5101 cgataaggat gagaaatcag atatggattt ttcctgaatt gttataaata attcaaaaga
     5161 aatttcagtt taatatctgt aattaaaaaa ccgatacttg ttttctccca attcgatctt
     5221 tccctactta ttatgaattg aaaaaattct aacggacatg gttggagaaa tgaaatgagt
     5281 tggataacag taagtcatca gaagaaaaaa cttcattctg ttcttgaatt caaaaaagag
     5341 gcaggaaaat ataatagaga aagagaaata gctcaaattg cgatgagcac gaattattta
     5401 tagtggcctc gatacgggct tcgccctact cggccaccta ccttcattga ctgtcgagta
     5461 atccgtcata gcgcatcgag ataagcgcct ttcaagtttt tttatagata attcaggata
     5521 aaaaagaaga ggggagtata ttcgatctca tcctatcttt tgaaagcctg ccaacatatt
     5581 ccagtgaggt catagactca gcgaaataca gaaaaaaatc taaatcaggc aattatggtt
     5641 cgatggatgg tagcctattt caaccattga tccagccaat cgaagaagac tcgttgccag
     5701 agcacagagt tttgaggttg caatacccaa tgattttcgt caggaaaata aaggaactcg
     5761 gctggtattc cccgaagcac agcggcatta aaggcgctca taccctgtga ggccacgatt
     5821 cgataatctt tttcactgtg aatgattaaa atcggtgtat cccatttatc tacaaagcga
     5881 tggggcgaat tggcaaacga gcgctgggca atggcattat ctttatccca gtaaggaccg
     5941 ccgaggtccc aatttacaaa ccacatttct tcagtttcga gatattgagc ttcgagattg
     6001 aacatgccat catgagcaat aaatgctttg aatcttttat tgtgatgccc tgccagccaa
     6061 taaacactga atgctccata acttgcgcca atagcaccca gatggttttc gtcgctccag
     6121 ggttcgctcg ttacatcgtc aatagccgat aaatagtcct tcatgttttg gccaccataa
     6181 tctcccgaga cctgttcctt ccattcctgt ccgaaacccg gtactccgcg tcggttagga
     6241 gctacaatta tatatccatt agccgccata agctgaaaat tccatcggta cgaccaaaac
     6301 tggctcgtag cattttgagg acctccttca caaaaaagca gggtggggta agattttgta
     6361 ctgtcaaaat gaggagggaa aataacccac gacttcatca tttttccatc ggtggttttg
     6421 gtccagcgtt cctcaacttt tcccagagta agccgagcaa ataaatcttt gttgatgaat
     6481 gaaatttcag taaattctcc attgctggga tcgatggaga agatttcgtc gggttgttgg
     6541 atactcattc taatcccata aagtttatcc cctccatcca aaactgtttt aaaatcatgc
     6601 attccatcgg taacttgccg gatttcgcca tctgccatag aatacgaata aatctgataa
     6661 gtagcctggt gattactgat aaaatagatc aggtcgccat gtttgctcca tgtaatctga
     6721 cttacatccc cgtcgaatcc ttgactggca tagatttttt cgccagtgaa taaattcaac
     6781 aaaaacaatc tttgttgatc ggcttcgtag ccgtctcttt ccatgctcca ccatgccaga
     6841 tatttaccat cgggtgaaaa tacgggttcc atgtcatagc ccatcattcc tgaagttaaa
     6901 ttagtagttt gtccagtcgc caacgaataa gcatacaaat cggtattggt gctcagggca
     6961 tattctttcc cctgtttttt ccgacatgtg taaataatgg ttttgctatc ggggctaatg
     7021 gctacttgtt ccattccacc gaatggtctt atgggcgatt cccatggttc gccattcatg
     7081 atgtctttgc cttggcttac tggctggccg ttaaaagttg taataaatat gtgcgaaaaa
     7141 gcatctaccc atgaatccca atgacgatac atcaagtcat tcatcaatcg gccgctggtt
     7201 ttgtcgagcc cttcgaaaag tttcttcttg attcgttgcc ggggaacatc cgaaacataa
     7261 accaacagcg acatatcggc tgaaaattta taaccattta ctccattctc aactcgagta
     7321 agctgtttgc ggtttttccc atttttatcc attatccaaa tttgtggtgt gcccgattcg
     7381 gaagaaataa aggtaatttt tccgtcgggg gtccatcggg cttcatactc cgaaaatggt
     7441 gtatgcgtaa tccggcggca attgtttcca tctgaattca tcaagtaaag ctcatggttt
     7501 cctttatttt tatccagatt ataccaagta actctaaaaa gaatttcact ggcgtcgggc
     7561 gagagttgaa tatcacttac ccgtccaata ttccacaata attcggcagt tagcaggtca
     7621 ttttcaagct gaagggtttt aggcccgata atttcatcgc tggtaccttt cttttcttgt
     7681 cttacacatg ccgtaaggct catgacgagg caaatagaaa taaaaagatt tcgattgatt
     7741 ttatacatgt tacgatgatt ttagttgatt aatctatcaa aaattatttt accggttttt
     7801 gatgacacaa atattgcaat aaaaattcat aaggcagaat aattttcgca tccatcatgt
     7861 aatcgtattg gaaattaatt cttaatgaaa tgaaactgca ataatgaatt gtattggtca
     7921 tggcaaatca ttcacatttc tgagctaagc agagcttttc gttatttttg cccgaaattt
     7981 tgacggaatg gaaagtttct ggaatctgct tattcataat atacagcgga ctgcacccct
     8041 cgaattcatt gcagtaggtt ttggtcttct aagtgttttc tttattatga aggaaaatat
     8101 atggggatac cctacgggtc ttttgaatgt gggactatat gtgtatttgt gtttcaaggc
     8161 cggcttattt gccaatatgg gaatcaacgt gttttatttt atcgtgagta tttatggatg
     8221 gtacaattgg gctcatccgg gagaacaaga gacccgattg cgtgtaaagc ggctgaatcg
     8281 caaagaaact ctaatagcca tctgcagcat gatgattctt ctctttttca tctggaggtt
     8341 attacgagat ttccccgaga gctcttatcc tttttttgat gcactaacga catcgatttt
     8401 tatcattgcc atggtacttc aagctttgaa gtgtctcgaa agctggattt tatggattgc
     8461 gggcgatttt atagctattc ccttgtttgc ttcgagcgga ttggccttta catgtttgca
     8521 ataccttgca ttcctcatta tggctatttc tggattcttt tcatggaaaa aatctctctt
     8581 gcgaaatgat taaaatttcc attaccgggc ctgaatctac cggaaaatct tggttatccg
     8641 aaaacctcgc ttatgttttt aatacgattt gggtgaaaga atatgcccgg gaatatctcg
     8701 aacgcctcga tcacccttat acaattgatg atgtgattac aattgctcgc aaacaaaggc
     8761 aactcgagca tgaaatagca agctataaac ctcatcttat gttctgcgat accgatatgc
     8821 tggtatgcaa aatttggtgc gaggtaaagt ttggctattg cccacctcaa attatcgagc
     8881 tatatcaaga agatgattat caactctatt tactgatgga cattgattta ccttgggtgt
     8941 acgatccttt gcgcgaacac ccacacttca ggcaagagct tttcaaaata tacttgaata
     9001 ctttgacatt agaccataag cccttcgaag tgataaaggg cttaaatggg gaaaggctta
     9061 taaatgccgt gaatgctatt atgcatcatt ttgtcggaat caaggaaatg ctaatcccag
     9121 aaagaatcca atcgtacgag taaaattcca tcacttcgag tggctttcat acctttgtac
     9181 ttcattcttc aaaagtgttc gtcatgtcgg tatccgataa tctaaaaaaa attctatcaa
     9241 ctttacctca aggggttaag ctcgtagcag ttaccaaaac tcatccgccc gaagtgataa
     9301 tggaagctta ccaagcaggc cataatatct ttggagaaaa caaggcacag gagatgcgct
     9361 caaagtatga
//
LOCUS       DAOORU010000098         8934 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_9660_length_8934_cov_5.505124, whole genome shotgun sequence.
ACCESSION   DAOORU010000098 DAOORU010000000
VERSION     DAOORU010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9660_length_8934_cov_5.505124"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLF20_07045"
     CDS             complement(<1..588)
                     /locus_tag="PLF20_07045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOX80790.1"
                     /translation="MKHAQFIIFIINLAYILGASGQEIQFGNPLTIPLRLSGTFGELR
                     SDHFHTGIDIKTNQQEGYPVIATEDGYVARIAVSPYGYGKALYINHPSGYTSVYGHLQ
                     RMNGTIATYLHRKHYEMQKFQLDFSLQPYEIKVRKGDTIAWSGNSGSSGGPHLHFELR
                     ETSSQAAVNPLQFGINVKDWIRPTITLLKVYPDDFG"
     gene            complement(585..1082)
                     /locus_tag="PLF20_07050"
     CDS             complement(585..1082)
                     /locus_tag="PLF20_07050"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOX80791.1"
                     /translation="MNNHIVNIKLVIFDWGNTLMRDNPELQQPMYMWGKVELIPSATE
                     ALEYLSKKYNLCVATNAGVSDTQAMIKALERVDIARYFQYFYSSIDLRVSKPLPAFFE
                     KICHEIGIPPSQTVIIGNSYDKDIVGAKQAGLHTIFFDEWKQANLNPDADRVIHHLKE
                     LTEIL"
     gene            complement(1176..3212)
                     /locus_tag="PLF20_07055"
     CDS             complement(1176..3212)
                     /locus_tag="PLF20_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008772597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOX80792.1"
                     /translation="MNKISYTLLLAFVMITACNQTQKKQPNEEFRWQIDRFADIKIMR
                     YQVPGWDSLSLQQKELIYDLSQAALCGRDIIFDQNYKYNLAIRRTLESIYENYRGERS
                     GADWDNFMVYLKRVWFSNGIHHHYSMDKFLPQFSKEYFLQLVADCPDNTLPLQVNQSK
                     DEFLGWISDIIFNPEIAPKRVCLDASKDLVTSSACNFYEGVTQKEVEEFYNSFKIPDP
                     QRPLSLGLNSKVVKKKGKIVEIPYVADGLYGPAIQKIIYWLEKAVNVAENENQKKGLL
                     MLIDYYRTGDLYTWDQYNILWAQDTSFVDYVNGFIEVYGDPLGRKATWESVVDFRNDE
                     ATRRTKIISQNAQWFEDHSPIDPRFKKPKVKGVSAKVITVAQLGGDCYPSTPIGINLP
                     NADWIRKEHGSKSVTMENITYAYDQASLGTGFLEEFCYSPEEVEMAKKYGPLADNLHT
                     DLHECLGHGSGQLLPGVSSEALKNYQSALEEARADLFALYYMMDPKMIELGLIPNLDV
                     AKTEYNAYIRNGLMTQLTRIEPGKNIEQAHMRNRQLIAAWCYQKGLPEKVIEKKVKDG
                     KTYVIINDYERLRSLFGQLLAEIQRIKSEGNYTAGKELVENYAVKVDQTLHKEILERY
                     KKLNLAPYGGFINPMMKPIYENGQLVDVEISYPDDYVEQMLYYSKNYSFLPIYN"
     gene            complement(3236..3991)
                     /locus_tag="PLF20_07060"
     CDS             complement(3236..3991)
                     /locus_tag="PLF20_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HOX80793.1"
                     /translation="MSGKEQKLELAFTPLLFRHDALSAPYVVVLIDILRATTSICTAF
                     AHGVEAMIPVASLEEARHYKEWGYIIAAEREGIKPDFADLGNSAFHFMKPKLRGKTIV
                     YSTTNGTQAFSVVGYTIDVVIGAFVNLSTLTKWLIEDGRNIYLLCAGWKGNFNLEDTV
                     FAGALAASLMQSSQIIIQSDAVMASIDLWNLAKNNLAEYLLKTEHSQRLKRLGLDDVI
                     PYSLTFDTCPVVPVARNGMIRLATQTNHEHFYD"
     gene            4321..4962
                     /locus_tag="PLF20_07065"
     CDS             4321..4962
                     /locus_tag="PLF20_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HOX80794.1"
                     /translation="MTKSKRLTSLHGIKSQKNLINRRSFEEGTTEANEAKVDYQIRYH
                     RIYDDNWDFRNANTKEYTHCFHSYPAMAIPQVARRIIENFGKKSKLLFDPYCGTGTSL
                     VEANLQGINAIGTDINPLARLIAAAKTTKIDIQVLDSFLQDFINYIFEINFEVENVKP
                     IFIPEIKNIDFWFCKGNEKNWESCLDILKTSLILCLSPINCKIQGTYNLYRNH"
     gene            complement(5233..5547)
                     /locus_tag="PLF20_07070"
     CDS             complement(5233..5547)
                     /locus_tag="PLF20_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80795.1"
                     /translation="MRFLSVGAYQYWSREDTIYNNKLYHILYLEQHCLSLVTFKYCEA
                     IKEDNLKRIYIIDCNCAACGENEVILYDFSKTIDNTVFVGVKGLWSGKLLNHNRNIFD
                     SR"
     gene            5762..5911
                     /locus_tag="PLF20_07075"
     CDS             5762..5911
                     /locus_tag="PLF20_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80796.1"
                     /translation="MFLGKLKAWVENTKYKQMVIGDGGIKNEKELFLYFFFFFLLAYF
                     PVSLA"
     gene            6006..7469
                     /locus_tag="PLF20_07080"
     CDS             6006..7469
                     /locus_tag="PLF20_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOX80797.1"
                     /translation="MIEDYFSPFRKNIVGNERMIETPFGCRKLIYADWIASGRLYKPI
                     EEKMLNVFGPWVANTHTETSETGTLMTKSYQYAHQLIKQHVHASPHDVIITYGFGMTG
                     VVNKLQRILGLKFCGRLFSSTCLAERDKPIVFITHMEHHSNHTSWFETAADVEVIGHT
                     PNLEVDLNDLEDKLKKYKDRKFKIASVTACSNVTGIQPPYYEIAKLIHSYGGLCFVDF
                     AASAPYVDINMHPDNPLEKLDAIFFSPHKFLGGPGSSGVLIFDSSLYHNPVPDQPGGG
                     TVDWTNPWGEYKYIDDIELREDGGTPGFLQAIRTALCIELKNRMGTPNIHQREQQLVK
                     KAFELFRRIPHVHILADEFEDRLGVFSFYIDNVHYNLVVKLLNDLAGIQVRGGCTCAG
                     TYGHYLLNVSYEQSKRITDKINQGDFSEKPGWVRLSLHPTMTNEELEIAAATTYEIAT
                     NIEYYREQYIYDPRKNEFRHRSEPIDKIVFVKDWFTL"
     gene            7632..>8934
                     /locus_tag="PLF20_07085"
     CDS             7632..>8934
                     /locus_tag="PLF20_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOX80798.1"
                     /translation="MMKESYVGLVNESLETNWDLKAFSDYEGGTYSFAEVASHIHYLH
                     QLYQKCGIKAGDKIAIYGRNSSNWAIAYLSVITYEAIVVPILPNFGPNEVHHLMNHSE
                     SCFVFASQSLWEALDASKMLTIMMSISLDDFSCLYATQDTFLDNVEEFNRIKSNLQDK
                     TCINWQVPSNDKLLAIIYTSGTTGFSKGVMLTHNSITANIVFSRNHMLLKPGDRILSL
                     LPLAHAYGCSIEFLYPFTLGCHITFLGKTPSPQVLMRAFSEVKPRLILSVPLILEKIY
                     KTQVKATLSTPRMKIFLGIPFIENIVYKKVLQKLLDFFGDNFIELIIGGAALNEEVQI
                     FLRKIRFPFTVGYGTTECGPLITYAPWYENRLNSCGRCVDTLEMKIDSADPQKEVGEI
                     LVKGENVMVGYYKNPEATRETFTNDGWLRTGDLGIMDSDGFL"
BASE COUNT         2631 a         1737 c         1771 g         2795 t
ORIGIN      
        1 accaaaatcg tcgggataaa ctttcagcag ggtaatagta ggccgaatcc agtctttcac
       61 gtttattcca aattgtaaag gattgacggc tgcctgtgaa gaagtttcgc gaagttcgaa
      121 atgaaggtga ggccctcccg acgagcccga gtttcctgac catgctattg tatcgccttt
      181 tctaactttt atttcataag gttgtaagct gaaatcgagt tgaaattttt gcatttcata
      241 gtgttttcga tggagatacg tggctattgt gccgttcatt ctttgcaaat gcccataaac
      301 actggtatat ccactgggat ggttgatata aagagctttc ccataaccat agggagaaac
      361 ggcaattcgg gctacatatc catcttcggt agcaatcacc gggtaacctt cttgctggtt
      421 cgttttgata tctatgcctg tatggaaatg gtcggaacgc agttcgccaa acgtacccga
      481 aagccttaac gggatggtca ggggattccc gaattggatt tcctggcctg aagctcccaa
      541 tatgtaagcc aggttaatta tgaagataat aaattgtgca tgtttcataa aatctccgtc
      601 aattctttta aatgatggat taccctatca gcatcagggt ttagatttgc ctgtttccat
      661 tcatcgaaaa atatagtatg aagcccggct tgttttgctc ctacgatgtc cttatcataa
      721 gaattgccta ttatgactgt ttgtgaagga ggtattccaa tttcatggca aattttttcg
      781 aagaaggcgg gaagaggttt gcttactctc aaatcgatag atgaataaaa atattgaaaa
      841 taacgtgcaa tatccacacg ttcgagtgcc ttgatcatgg cttgtgtatc gcttactcca
      901 gcattggttg ccacacataa attatatttt ttcgagagat attccaaggc ttctgtggcg
      961 ctgggaatta attctacctt tccccacata tacatgggtt gctgaagttc agggttatcg
     1021 cgcatcaagg tatttcccca atcgaaaata acaagtttta tattgactat gtgattgttc
     1081 attccgaaat tttgttacgg caaattcacg catggaggac ggaagatcga agaatttccg
     1141 acgccctcct gcatgcaatt caaagaaatg ttcaattaat tgtatattgg taaaaacgaa
     1201 taatttttcg aataatagag catttgttcg acgtaatcgt cgggatagga aatttcgacg
     1261 tcaaccaatt ggccattttc atatatgggt ttcatcatag ggttgataaa tcctccataa
     1321 ggagcgagat tcaatttttt atagcgttcg agaatttcct tatgcaaagt ttggtcaact
     1381 ttgacggcat agttttctac caattccttg cctgccgtgt aatttccttc cgatttgatg
     1441 cgctggattt ctgcaagtaa ttgtccaaaa agagaacgca aacgttcata atcattgata
     1501 attacgtagg tttttccatc ttttaccttt ttttcgatga ctttttcggg taggcctttt
     1561 tgataacacc aggcagcaat aagctggcgg ttacgcatgt gagcctgttc gatgttttta
     1621 ccgggttcga tacgggtgag ctgagtcatc aatccattcc gaatataggc attgtactcg
     1681 gttttggcaa catctaggtt gggaatgagt ccgagctcta tcatttttgg atccatcatg
     1741 taatataatg caaacaaatc ggcacgagct tcttcgaggg cagattgata atttttgaga
     1801 gcttcgctgc ttacgccagg taaaagttga cctgaaccat ggccgaggca ttcatgaagg
     1861 tcggtgtgaa gattatcggc caagggccca tatttttttg ccatttctac ttcttcggga
     1921 gaataacaaa attcttcgag aaatcccgtt cctaacgaag cctgatcgta agcataagta
     1981 atgttttcca tggtaaccga tttcgaacca tgttctttgc gaatccaatc ggcatttggc
     2041 aaattgatcc caataggagt cgagggataa caatcgccac ccaattgtgc taccgtaatc
     2101 actttggccg agactccctt aacctttggt ttcttgaaac gaggatcgat gggggaatga
     2161 tcttcgaacc actgtgcatt ttggctgatg atcttggtac ggcgtgtggc ctcatcattc
     2221 cgaaaatcta ctaccgattc ccatgtggcc ttgcgtccca gaggatcgcc ataaacttcg
     2281 atgaaaccat ttacgtaatc tacgaaggaa gtgtcctgtg cccaaagaat gttgtactga
     2341 tcccatgtat ataaatcgcc cgttcggtaa taatcgatga gcatgagtag accttttttc
     2401 tggttttcgt tttccgccac atttaccgcc ttctcgagcc agtaaatgat cttttgaatg
     2461 gcaggcccat aaagtccatc ggcgacataa ggaatttcaa cgattttacc tttctttttc
     2521 acgactttcg aattcaatcc gagcgaaaga ggccgttgtg ggtcaggaat cttaaaacta
     2581 ttgtaaaatt cttctacttc tttttgagta actccttcat aaaaattgca ggccgaagag
     2641 gttactaaat ctttcgaagc atcaagacaa acacgttttg gggcaatctc aggattaaag
     2701 atgatatcag aaatccagcc caaaaactca tccttcgatt gattcacttg aagagggaga
     2761 gtattatcgg ggcaatcagc cactaactgc agaaaatatt ccttactgaa ctggggtaag
     2821 aacttatcca tagaataatg atgatggata ccatttgaaa accacaccct tttcaagtaa
     2881 accatgaaat tgtcccagtc ggctccactg cgctcgcctc tatagttttc gtagatggat
     2941 tcgagcgtgc gtcgaatagc aaggttatat ttataattct ggtcaaaaat gatatcgcgt
     3001 ccgcaaagtg ctgcctgact caaatcataa atgagctctt tttgctgaag acttaaacta
     3061 tcccagcctg gaacttgata acgcataatt ttgatgtcgg caaagcgatc gatttgccaa
     3121 cgaaattcct cgtttggttg tttcttttgg gtttggttgc atgctgtgat cataacgaag
     3181 gcaagtaata aagtgtaaga aatcttattc ataatatgaa gatttaaaaa cggttttaat
     3241 cgtaaaaatg ttcatgattt gtttgcgtgg caagacgaat catgccgttt cgagccacgg
     3301 gtactacagg gcaggtatcg aaggtaagag agtagggaat aacatcgtcg agccccagtc
     3361 gttttagcct ttggctatgt tcggtcttga gaagatattc ggcaaggttg tttttagcca
     3421 ggttccataa gtcaatagaa gccataacgg catcgctttg gataattatt tgactcgatt
     3481 gcataagaga tgcagccagt gctccagcaa acacagtgtc ctccaaattg aaattgcctt
     3541 tccaacctgc acataaaagg taaatattcc tgccatcctc gatgagccat ttggttagag
     3601 tgcttaaatt cacaaaagca cctattacta catcgatagt ataaccaaca acagaaaatg
     3661 cctgagttcc gtttgtagtg ctatacacta tggtttttcc tcttaatttg ggtttcataa
     3721 agtgaaaggc tgagttacct aggtcggcaa aatcgggttt aattccttca cgctcagcgg
     3781 ctataatgta tccccattcc ttataatgtc ttgcttcttc gaggcttgcc acgggaatca
     3841 tggcttccac tccatgagca aaggccgtac aaatagaagt tgtagccctc agaatatcta
     3901 tcaacacaac cacatagggt gcagacaatg catcatgccg aaatagtaaa ggcgtaaatg
     3961 ccaattcgag tttttgttct tttccggaca tctatctctc aaattatttg gcctggctaa
     4021 attacaaaac acctcggaag taaaagcttc accttcttcc tgagcttact ttattcattc
     4081 atattcaatt aatttgttaa ttgcatgaga ttttttcagt gagctttatc tcagagattg
     4141 ccaaaacttt tcccagtata atttttgatg gtaatagaaa gtttttcaat gcttttggaa
     4201 cactcatttt aatcaatatt gctataattc tttgaattaa ctggagttat attcaatggc
     4261 aaattttaat ttatttgata atgattgttt atcataaagg ttaaaagatg aaattgcatc
     4321 atgacaaaaa gtaaaaggct tacttcgtta catggaataa aatctcaaaa gaatttgatt
     4381 aacaggagat catttgagga gggaacaaca gaggcaaatg aggctaaagt tgattatcaa
     4441 atacgttatc ataggattta tgatgataat tgggatttcc gaaacgcaaa cacaaaagaa
     4501 tatacgcatt gctttcactc ctatcctgca atggcaattc ctcaagtggc aaggagaatc
     4561 atcgaaaatt ttggtaaaaa atcaaaacta ctatttgatc catattgtgg tactggaacc
     4621 tccttggttg aggctaactt gcaaggaata aatgcaatag gaaccgatat caatccttta
     4681 gctcggctta ttgcagccgc aaaaaccacc aaaattgaca tccaagtttt ggattcattc
     4741 cttcaggact ttattaacta tatcttcgaa atcaattttg aagtagaaaa tgtaaaacct
     4801 atctttattc cagagatcaa gaatattgac ttttggtttt gtaagggtaa tgaaaaaaac
     4861 tgggaatctt gcttggatat attgaaaaca tcgctgatcc tgtgtcttag ccccataaat
     4921 tgtaaaattc aaggaactta taacctctat agaaatcatt gagttatgct tgttcctatt
     4981 accggatggt gaaatatatt taacttggat ttttccgaat gagaaggaac tccttcaaat
     5041 aacagaaaac aagtttcata atcaggattc agatatttta cggtatcatt ttgcctcata
     5101 catattaatt tcttcacaat aaaacctgta gggaaagggt aaataggcga aagcaacccg
     5161 aaggtactac caatactatt catccatttt tctacatact cgtaaaagtg aaatctcttt
     5221 ctgcatccat cttcaacgag aatcgaatat atttcggtta tgattcaata gcttgcccga
     5281 ccaaaggcct ttcacaccaa caaaaacagt gttgtcaatg gttttcgaaa agtcatataa
     5341 tattacctca ttttctccac acgcagcaca attgcaatcg attatataaa ttcgtttcaa
     5401 attgtcttct ttgatagctt cgcaatactt aaaagtaact aaggataaac agtgttgctc
     5461 aagatataat atgtggtaaa gcttgttgtt ataaatagtg tcctcacgag accaatattg
     5521 ataggctcct accgacaaaa accgcatgaa ttgttctatt ttactccata tcttattgtg
     5581 actctttctc caatttgttt cggagtttca tcaaggcgag tcgagagcct ttctattaaa
     5641 agggatgaat atgcgatgat ggggtaattt ctcttttttc ttcaaaaaac tcattgaatt
     5701 tcaaaaattc aagtgatagg ataatttttc tatttgtcaa agtttagtaa aaaagttgaa
     5761 aatgttttta ggaaaattaa aagcttgggt tgaaaataca aaatataaac agatggtaat
     5821 tggcgatggg ggaataaaga atgaaaagga gttattttta tatttcttct tctttttcct
     5881 attagcatat tttcctgttt ccttggctta aaatccccaa tgtttgtgca ctccttgcaa
     5941 cattatattt catactcctt cacaaaaacc tgtaaatttg ccccattttc cgaatcattc
     6001 gttttatgat tgaagattac ttttcgccat ttcgtaagaa tatagttggg aatgaaagga
     6061 tgatcgagac tccttttggg tgcaggaaac tcatctatgc cgattggatt gcaagcggca
     6121 ggctctacaa acctatcgaa gaaaaaatgt tgaatgtttt cggcccctgg gtagccaata
     6181 ctcataccga aaccagcgaa actgggacac tgatgaccaa atcgtaccaa tatgctcatc
     6241 agttgatcaa acaacatgta catgccagtc ctcatgatgt catcatcact tatggttttg
     6301 gaatgacggg tgttgtaaat aaattgcagc ggattcttgg attgaaattt tgtggacgtt
     6361 tattcagttc cacctgtctt gctgaaaggg acaaacccat tgtgtttatt acgcacatgg
     6421 aacatcacag caatcatact tcatggttcg aaaccgcggc cgatgtcgaa gtgattggtc
     6481 ataccccaaa cttggaagtc gatttaaatg atctggaaga caagttaaaa aagtataaag
     6541 acagaaaatt taaaattgcc tcggtcacgg cttgttcgaa tgttacggga atacagccac
     6601 cctattacga aattgccaaa ctcattcata gttatggagg tttgtgtttt gtagattttg
     6661 ccgcttcagc tccttatgta gatattaaca tgcatcccga caacccgctc gagaagctcg
     6721 atgctatttt cttttcgccc cataagtttc ttggtggtcc cggaagcagc ggtgtattga
     6781 ttttcgattc atcactctat cataaccctg tccccgatca gcctggagga ggaacagtgg
     6841 attggacaaa tccatgggga gaatataaat atatcgacga catcgaactt cgtgaagatg
     6901 gaggcactcc aggtttttta caagccatcc ggacagcttt gtgtatcgag ctgaaaaatc
     6961 gaatgggaac cccaaacatt catcaacgtg aacaacagct cgttaaaaag gccttcgaat
     7021 tatttcgtcg aatccctcat gttcatattc tggccgatga attcgaagat cgactgggcg
     7081 ttttttcgtt ttatatagat aacgtacatt acaatttagt cgtaaaactc ttgaacgatt
     7141 tggctggaat acaggtaaga ggtggctgca cttgtgccgg tacatatgga cattatttat
     7201 tgaatgtaag ttacgaacaa tcgaaacgca ttaccgataa aatcaatcag ggcgattttt
     7261 cggaaaaacc tggctgggtc aggctttcac ttcatcctac aatgacgaat gaagagctcg
     7321 aaattgcagc ggctacaacc tacgaaattg ctaccaatat tgagtattat cgagagcaat
     7381 acatttatga tccaaggaaa aacgaatttc ggcatcgttc tgaacccata gataaaattg
     7441 tttttgttaa ggattggttt actttataaa atttttcttc aagatttttt cattgataaa
     7501 gacttatttc tattttttgg tgtatcttta ttctttggtc tctattccaa attgaaagaa
     7561 ttcctgaaga ttacgcttat tcgatcaaat actttacttt agcattttac tatttctaaa
     7621 atcctatatc catgatgaaa gaatcatacg tcggacttgt aaatgagagt cttgagacca
     7681 attgggatct caaggccttt agcgattatg agggtggcac ctattctttt gccgaggttg
     7741 ccagtcatat ccattattta catcagctct atcaaaaatg cggaataaaa gcaggggata
     7801 aaatagccat ttatggtcgg aattcgagca actgggcaat tgcttatttg tcggttatta
     7861 cctacgaagc catagtagtc cccatactgc ctaactttgg gcctaacgaa gttcatcatt
     7921 tgatgaatca ttcggagagt tgcttcgtat tcgcttcgca gtctctatgg gaagctttgg
     7981 atgcttcgaa gatgcttaca ataatgatga gcatttcttt agatgatttt tcctgtcttt
     8041 atgcgactca ggatacattt ctggataatg tcgaggaatt caatcggata aaatcgaatc
     8101 tacaggataa aacatgtatc aactggcagg ttccatccaa cgacaagctt ctggccatta
     8161 tttacacttc tggaaccact ggtttttcga agggggtaat gctgacacac aatagcatta
     8221 cagctaatat tgttttttcg agaaatcaca tgctcttgaa gccaggcgac cgtattctga
     8281 gcttattgcc actggcgcat gcatacggat gttctatcga atttttatac ccctttactc
     8341 tgggttgtca tatcaccttt cttgggaaga cgccatcacc tcaagtattg atgcgggctt
     8401 tttccgaggt taaaccccgt cttattcttt ctgttcctct catactcgag aagatttata
     8461 agactcaggt aaaagcaaca ctttctaccc cacgcatgaa aatatttctc ggcattcctt
     8521 tcatcgaaaa catagtgtat aagaaagtcc ttcagaaact cttggatttt ttcggtgata
     8581 acttcattga actcatcatt ggaggtgcag cacttaacga ggaggttcag atcttcttga
     8641 ggaagataag atttccattt acggtaggct atggaactac ggaatgcggt ccactgatta
     8701 cttacgctcc ctggtacgaa aatcgcctta attcgtgtgg tagatgtgtg gataccttag
     8761 aaatgaaaat tgattcagcc gatcctcaga aagaagttgg cgaaattttg gtaaaaggag
     8821 aaaatgtgat ggtgggctat tataaaaatc ctgaggctac tcgggaaact tttaccaatg
     8881 atggatggct gcgtacgggc gatctgggca tcatggattc cgatggattt ctct
//
LOCUS       DAOORU010000099         8822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_9823_length_8822_cov_5.524353, whole genome shotgun sequence.
ACCESSION   DAOORU010000099 DAOORU010000000
VERSION     DAOORU010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9823_length_8822_cov_5.524353"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(282..983)
                     /locus_tag="PLF20_07090"
     CDS             complement(282..983)
                     /locus_tag="PLF20_07090"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOX80799.1"
                     /translation="MKQKKISRLFFILIISFSVFISKESEAQVFRGILIGGFNLSQVD
                     GDETYGFHKLGANIGAGVMLPFTRKWSVTLETFLSQKGSHQDAFYVDTLPTGNILTGE
                     YDLRMNYVEIPLLIHFTDKETVSAGVGVSYGRLIGVTEKEHGKKVESTSVNNGPYKKD
                     DWDIIGDIKLPLYKNLKINLRYSYSLAKIRTRDFYDRNGEYLKTRDQYHNLFSIRFLW
                     VINEKTRTLQEQKNE"
     gene            complement(1143..3275)
                     /locus_tag="PLF20_07095"
     CDS             complement(1143..3275)
                     /locus_tag="PLF20_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HOX80800.1"
                     /translation="MPSPLSRLIAKELNLTEQQVENVINLLQEGASIPFIARYRKELT
                     GSLNELSIIAIRDRLKQLTELEKRRDSVIHSVREQGKLTEELEQALRRATTLTEIEDL
                     YLPFRPKRKTRASMAIEKGLEPLAKIILAQKEEDILRLAKRFIDVEKGVENEDEALQG
                     ARDVIAEWVNQDAVVRQRLRRLFRSEAVIASRLVKGKDEEAQKYRSWFHWEEEVRRIT
                     SHRLMAILRGEAEGYLKVEIQPSLAKALEIIERIYMKARNMSSAQVRLALYDAYKRLL
                     QPSLETEIRSELKLKADIEAIGVFSENLRQLLLAPPLRGKNVLAIDPGFRTGCKVVCL
                     DQHGNLLHNETIYPHPPENKVKESMHKIKNLVNAYKIDAIAIGNGTAGRETERFIRHI
                     PFEKDIIAIVVNEAGASIYSVSDIAREEFPDYDVTVRGAVSIGRRLIDPLAELVKIDP
                     KSIGVGQYQHDVDQKMLQESLHDVVESCVNSVGVDVNHASKELLTYISGIGPVLAKNI
                     IEYRKENGPFPSRQQLLEVPRLGAKAFEQSAGFLRIPDAPNPLDTSAVHPESYPMVEA
                     MAAKLGVSVNELIQNKELQQSINIEDFVTEKCGIPTLTDIMKELAKPGRDPRVNFDFF
                     EFDKNVHQIEDLTVGMILPGIVTNITRFGVFVDIGVHQNGLVHISQLSKNYVSDPSQV
                     VRLNQKVKVKVLEVDVARSRISMSMKDV"
     gene            complement(3438..4241)
                     /locus_tag="PLF20_07100"
     CDS             complement(3438..4241)
                     /locus_tag="PLF20_07100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HOX80801.1"
                     /translation="MMKKFLIVLLCLVMAACQVTKKSSIQGHDDNDHLIMATLWFQQA
                     AEVRALYYQAFHLAQLQLEHDLAFGDNSTKRAVVLDIDETVLDNSPHEGYLIQNGKSY
                     PEGWSEWVSLAQAAALPGALDFINFAISHDVDVFYITNRKKDELNVTILNLRRLGFSM
                     ADSSHILVRTDGANKENRRQRVRQTHRIVLLIGDNLNDFSNLWEKTLPQRRNFLADSL
                     QYEFGKRFIILPNPMYGDWENALYQYNYHLSPSQRDSIRRSFIKAFSPL"
     gene            complement(4252..4944)
                     /gene="mtgA"
                     /locus_tag="PLF20_07105"
     CDS             complement(4252..4944)
                     /gene="mtgA"
                     /locus_tag="PLF20_07105"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOX80802.1"
                     /translation="MKKLMRRVLKWIRNIILILIGLSLFFTLLYKFVPVYYTPLMFIR
                     YFENVKAGKEARIERNWKRLDEISPNLWLAVVASEDNNFLKHHGFDMDAIKKAQKHNQ
                     THARKRGASTISQQVAKNVFLWPSRTWVRKGLETYFTFLIETFWSKKRILEVYLNVIE
                     IGPGIYGAEAASQIYFHKPASKLTRGEAALIAAVLPAPLRRNPAKPSPYVLSRQQWIL
                     WNMNNIGKIEFD"
     gene            5211..6365
                     /locus_tag="PLF20_07110"
     CDS             5211..6365
                     /locus_tag="PLF20_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOX80803.1"
                     /translation="MKNFVFYNPVKIYFGEGQISKLSNEIPKDATVLMTYGGGSIFRN
                     GVYEQVKLALANYKVLEFGGIEPNPRYETLIKALPIIKENKVDFLLALGGGSVIDGTK
                     FISAASFYEGDPWDFISKGIRVQKALPIGVVLTLPATGSEMNSGAVISKESTKEKCSF
                     FSPLVFPRFSVTDPSFTFSLPPRQVANGIVDAFIHTMEQYLTYPVSAPLQDRMAESIL
                     NTLMEEGPKAFTKPYDYDTRANIMWCATLALNGLIACGVPTDWATHGIGHELTALHGL
                     DHALTLAIVFPGVMKVMQDEKREKILQYGERVLKITQGTEKERIEQAILKTDEFFRSL
                     GMKTRLSENGIDQDAIETIVERFSKPRMICLGENQSITPVKIRQILLDRL"
     gene            6390..7961
                     /locus_tag="PLF20_07115"
     CDS             6390..7961
                     /locus_tag="PLF20_07115"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOX80804.1"
                     /translation="MKLFKNFLFRSFLIILFILRIFSIVEAQEEGISPEIIKNHIDFL
                     ASDKLQGRKTPSAGLDTAASYIAHELQNLGINSLNGTYFHSLNLCRCGLGKRQHFELN
                     TPSGKVVVPLKTGYIPFQTIGDTTVEGVLVFAGYGLNLPNLGYDDYSNIDVKGKIVVV
                     LSHFPGETSDDHRAKLIEKSGKTSINEKIATARNKGAIGLILLTNPKNHLLLKPQGHT
                     WAALLKRAKEDSDPLILKFHKESFTAIHAGEETMKLLFGSKENLIKIQEKIDKNFQPY
                     SFEIPGFTASIATSLHKRFIKADNVIGILEGNNPKLRSQYLIIGAHYDHIGTMKSEGT
                     DNSDTIFNGADDNASGTAAVLAIARAFAKAEKLPERSVVFVFFAGEELGLLGSRSFVL
                     DPPLPLDSIIAMLNFDMVCRGGSDTLYISPESTNSALYLMAKEKAMAEGFKTVQQNMI
                     SFGGSDHQPFLDKNIPALHFFTGLHADYHQVSDNPDRCDSVKASKVARLGWKLAWEIA
                     NSNITFANHYSEIKN"
     gene            8064..>8822
                     /locus_tag="PLF20_07120"
     CDS             8064..>8822
                     /locus_tag="PLF20_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80805.1"
                     /translation="MKHQHLALLLLFLSPLMGIAQKKYNPEVKLNKGDSITYLIQTNV
                     DMTQSMMGQEMKMNVSTQSKNIIAVSDIDKDKYYHLSQLSKEMTVKMKMDQMDSTMLV
                     PDITNLPVLVMSKKGTILRKSVSTNEKGQTMGNDFGDVTASSPFIEFPDRKISEGESW
                     ETLQNDTVNFMGGDLITNSKTVYTLIGTDEKEGMKCLKINHKTTADNEGTTTMQGMDF
                     LIEGTTIMEGTTWIDLKTAIPVFIDGTAESQMTLL"
BASE COUNT         2651 a         1822 c         1748 g         2601 t
ORIGIN      
        1 agccactatt gatgcggcaa tggatttcga aatgcgaacc ataaagattt attcaagccg
       61 tgctaaatag ttggacgagc ctcatgaaga agaattgtac cgaattcatg ctggttgcag
      121 gaaaggccat catgagttgc tcgaacggat taatgatgaa gtgaaaagta ccaatataga
      181 attataaccg attctggcca ttctgaaaaa ctttcacact cgattcattc ttattttact
      241 tgcctttgct tgttatcctg atgcagccct cactgattag gctactcatt cttctgttcc
      301 tgcaaggtac gtgttttttc gttgatgacc cacaaaaaac gtattgagaa caggttgtgg
      361 tactggtcac gggttttgag gtattcgcca ttacgatcgt agaaatcccg tgtgcgaatc
      421 ttggcaagcg aatacgaata tcttagattg atttttagat ttttataaag tggtaactta
      481 atatctccaa taatatccca gtcatctttt ttataagggc cattatttac agaagtagat
      541 tctacttttt tgccatgttc cttttccgta acgcctatca aacggccata gctaacaccc
      601 acgccggcac ttacagtttc cttatccgta aaatgaataa gtaagggtat ttcgacgtaa
      661 ttcattctca aatcatattc tcctgtcaga atattacctg tggggagcgt atccacataa
      721 aacgcatctt gatgagatcc tttctgtgaa agaaacgttt cgagggtcac cgaccatttt
      781 cgggtaaagg gcaacattac ccctgctcct atatttgctc ctaacttgtg gaagccgtag
      841 gtttcatctc catcaacttg gcttaggttg aagcctccta taagaatacc cctgaatact
      901 tgggcttctg attcttttga aatgaacacc gaaaaactaa taattaggat aaagaataac
      961 cgggaaattt ttttttgctt catagaaaga cggctttgat agcaacaaat tttattagtg
     1021 atgtagcgaa aatttttgca atcgagttgt tcgttcgacg aataaattcg ttggcaaaag
     1081 taccgaatta aaaccattag gccgaaagat aaaaacagat gaagaagcaa tggagatata
     1141 aattacacat ctttcatact catgctaata cggcttctgg caacatcgac ctccagtact
     1201 tttaccttta ctttttgatt gagacgaact acctgtgaag gatcactgac ataattcttg
     1261 ctcaattgac tgatatgtac cagcccgttc tgatgcactc caatatctac gaaaacaccg
     1321 aagcgagtaa tatttgtaac aataccaggc aaaatcattc ctactgtcaa gtcttcaatt
     1381 tgatgaacgt ttttatcgaa ttcgaaaaaa tcaaagttta cacggggatc gcggccaggt
     1441 ttagcaagtt ccttcataat atcggtgaga gttgggatgc cacatttttc tgtcacaaaa
     1501 tcctcaatat tgatactttg ttgaagttct ttattttgga tgagttcatt gacactcact
     1561 ccaagttttg ctgccattgc ctcgaccatg ggatagcttt cggggtgaac ggcactggta
     1621 tcgagagggt tgggtgcatc gggaatacgt agaaatcctg ctgactgttc gaaggctttt
     1681 gctcccaatc ttggcacttc aagcagttgc tgtcgcgagg gaaatggacc attctccttt
     1741 ctgtattcaa taatattttt ggctaaaacg ggtccaattc ccgaaatgta ggtcagcaat
     1801 tccttacttg catggtttac atcgactcca acgctgttta cgcagctttc taccacatca
     1861 tgcaaggatt cctgaagcat tttttggtcg acatcgtgtt gatactgtcc cactccaata
     1921 gatttgggat cgatcttcac cagttctgcc agaggatcga taagcctgcg gccaatagag
     1981 actgcgcctc tcacggtgac atcataatcg ggaaattctt cacgggcaat atcagaaacc
     2041 gagtaaattg aagctccggc ttcattcacc acaattgcga taatatcttt ttcgaagggg
     2101 atgtggcgga tgaatctttc ggtttcgcgg cccgcagttc cattaccaat ggctatagca
     2161 tcaattttat aggcattcac cagattcttg atcttgtgca tgctttcttt taccttgttt
     2221 tcgggaggat gaggatagat ggtttcgttg tgtaaaagat tgccatgctg atcgaggcaa
     2281 accaccttac atcccgttcg aaaacccgga tctattgcca gaacgttttt ccctcttaat
     2341 ggaggtgcaa gtaacaattg gcgtaagttt tccgaaaaaa ccccgatggc ttcaatatca
     2401 gccttaagtt ttaattccga gcggatttcg gtttcgagtg aaggttgcaa aagacgctta
     2461 taagcatcgt ataaagccaa gcggacctga gccgatgaca tgtttctcgc cttcatataa
     2521 attctttcga ttatttcgag tgccttagcc agagagggtt gaatctccac tttcaggtat
     2581 ccttcggctt cacctcgtaa aatcgccatc aaacgatgcg aagtaatcct tctcacttct
     2641 tcttcccaat gaaaccatga acggtatttt tgcgcttcct cgtccttacc tttcaccaag
     2701 cgtgaagcaa ttactgcttc acttcgaaat aacctgcgca atctctgacg taccactgca
     2761 tcctgattta cccattcggc aatgacatcg cgagctccct gcaatgcctc atcttcattt
     2821 tcgactccct tttcaacatc gataaacctt tttgccaacc ttaatatatc ctcttctttt
     2881 tgtgccagta tgatttttgc caatggttct aacccttttt cgatagccat gctggctctg
     2941 gtttttcgtt tgggacgaaa aggcagatat aggtcttcga tttcagttaa agtggtagcc
     3001 ctgcgaagtg cttgttccag ctcttcagtc agtttccctt gttctcttac cgaatgaatt
     3061 accgagtcac gtcgtttttc aagttcggta agctgtttga gcctgtcgcg tatggcaata
     3121 atactcagtt cgttaagact gcccgtaagt tctttcctgt aacgcgcaat gaaaggaatg
     3181 ctggctcctt cctgaagcag gttgatgacg ttttcaacct gttgttcagt taaattcaat
     3241 tctttagcaa tcaatcgaga aaggggtgaa ggcatttttc ctatttttta caaagatagt
     3301 ctccttgatt ttaacagaaa ttcgttgact ttttggagtt attttcaatc gaaaaaaatt
     3361 attatccaaa aggaaataaa ccagttttcg agttttacac gacattcttc aagatgatgc
     3421 cttctcgtgc taaaaaatta taatggtgaa aaagccttta taaatgaacg acgtatggag
     3481 tctctttgag aaggggacag atgataattg tattgataga gtgcgttttc ccagtctccg
     3541 tacataggat tgggtaaaat gatgaagcgc ttgccaaact catattgcag tgaatcggca
     3601 aggaagttgc gacgttgggg caaagttttt tcccataaat tactaaaatc gttcaaatta
     3661 tccccaatca ataaaacgat tcgatgagtt tgacgaactc tctgccggcg attctccttg
     3721 ttagccccgt cggttcttac gagtatgtgc gaagaatcgg ccatagagaa ccctaatcgc
     3781 cgaagattga ggatggtaac attcagttca tcttttttac ggttagtgat ataaaaaaca
     3841 tcaacatcat ggcttatggc aaaattgata aagtcgagtg cacctggaag agcggcggcc
     3901 tgtgccagag aaacccattc actccatcct tctggataac ttttcccgtt ttgaatcaaa
     3961 taaccttcat gagggctatt atcgagcacg gtttcatcaa tatcgagtac cacggctctt
     4021 tttgttgagt tatctccaaa ggcaaggtcg tgctcgagct ggagctgagc caaatgaaaa
     4081 gcctgataat ataaagcacg aacttcggcc gcttgctgaa accaaagcgt cgccataatc
     4141 aaatggtcat tatcgtcgtg tccctgaatt gacgattttt tggtaacctg acaggcggcc
     4201 atgactaaac ataataaaac tattagaaat tttttcatca tataagtaaa ttcaatcgaa
     4261 ttcaatttta ccgatattat tcatattcca aagaatccat tgttgacgtg aaagtacata
     4321 aggagatggt ttagcaggat ttcgcctgag aggagccggc aacactgccg caataagtgc
     4381 agcctctcca cgtgtaagtt ttgaagcagg cttgtgaaag taaatctggc tggctgcttc
     4441 tgccccataa atcccagggc ctatctctat tacattcaaa taaacttcga gaattcgttt
     4501 tttgctccaa aaagtttcta tcagaaaggt gaaatatgtc tccaatccct ttcgaaccca
     4561 agtacgagat ggccataaaa aaacgttctt agctacctgt tggctaatgg tacttgctcc
     4621 tctttttctg gcatgcgtct gattgtgttt ttgggccttt tttatggcat ccatgtcgaa
     4681 tccatgatgc ttcaaaaaat tattgtcttc cgaagctacc accgccaacc ataaattggg
     4741 cgagatctca tccagtcttt tccaattcct ttctattctt gcttcctttc ctgccttgac
     4801 attctcgaaa tacctaataa acatcaatgg agtataatat acgggaacaa acttgtagag
     4861 tagagtgaaa aataaagaca aaccaataag aattagaata atattcctga tccactttaa
     4921 aacacgtctc atcaattttt tcataggggc aaacaagctg aatctgaccg aaagtcatct
     4981 ttcgtgcaaa aaagataaaa agaaatgatt acacaaaatt aaggaggata gcctaagatt
     5041 ccgctgatgt catgaaaaat gattgattaa ggaagatatg ctatctacac ctcgatcgat
     5101 caatcgaatc acaattaaaa taattctctc gatgaaatcc tattattttt gcaactaaca
     5161 tagtgaataa taactttaca tcgaaaaatt gaaatcgaaa aacccacaca atgaaaaatt
     5221 tcgtttttta caatcctgtg aaaatatatt ttggggaagg acaaatttcg aaactttcaa
     5281 atgaaattcc gaaagatgcc actgtgttga tgacttacgg tgggggcagt attttcagaa
     5341 atggggttta tgaacaggta aagttggcct tggccaatta taaagtactc gagtttggag
     5401 gtatagagcc caatccacga tatgagacat taatcaaggc tttacctatc atcaaggaaa
     5461 ataaagtaga ttttttatta gccctgggcg gtggctctgt tatcgatgga acgaaattca
     5521 tttcggcagc gagtttttat gaaggagatc cctgggattt tatatcaaaa ggcataaggg
     5581 ttcagaaagc tcttccgatt ggtgtagtgc ttactttgcc ggcaacggga agtgagatga
     5641 attcaggagc ggtaatctcg aaagaaagca ccaaagaaaa atgttctttc ttcagtcctt
     5701 tggttttccc acgtttttcg gtcaccgatc cttcatttac cttttccttg ccgccacggc
     5761 aggtagccaa tggtatcgtt gatgccttta ttcacaccat ggaacaatat cttacttatc
     5821 ctgtgagtgc acccttacaa gaccgcatgg cagaaagcat actgaatact ttaatggagg
     5881 aaggtccaaa agctttcaca aaaccctatg attatgacac acgtgcaaat atcatgtggt
     5941 gtgctacctt ggcgttgaat ggattgatcg cttgtggtgt tcccaccgat tgggctactc
     6001 atggcatagg gcatgaactc accgctctgc atggtctcga ccatgccttg acgcttgcca
     6061 ttgtctttcc cggcgtgatg aaggtgatgc aggatgaaaa acgcgaaaaa attcttcagt
     6121 atggtgaaag agtcttaaaa attactcaag gaaccgaaaa ggaaagaata gagcaggcca
     6181 tattaaaaac cgatgaattc ttccgctctt tagggatgaa aacccgcctg agtgaaaatg
     6241 gtatagacca ggatgccatc gaaaccattg tcgaacgctt ttctaaacct cgaatgattt
     6301 gtttgggcga aaaccaaagc attactcccg taaaaatccg tcagattttg ttggatagac
     6361 tttaatcaat gaactccaat gtataattta tgaaactctt taaaaatttt ttatttaggt
     6421 cgtttctcat catcttgttc attctgagaa tattttccat agttgaagca caggaggaag
     6481 gcatttctcc cgaaatcata aagaatcaca tagattttct ggcatccgac aagctgcaag
     6541 gacgaaaaac tccaagtgca ggtctggata ctgctgcaag ctatattgct cacgaattgc
     6601 aaaatcttgg aattaattcc ctgaacggca cttacttcca ttccttgaat ttatgcagat
     6661 gtggtctggg caaacgacag cacttcgaat tgaatacacc ctcagggaaa gttgtcgtcc
     6721 cgttgaaaac aggttatatt ccttttcaga ctataggcga tactaccgtt gaaggagtat
     6781 tggtctttgc aggttatgga ctcaacctgc ccaatttggg atatgacgat tacagcaata
     6841 ttgatgtaaa aggtaagatt gtggtggtac taagccattt tcctggtgaa acttctgatg
     6901 atcacagagc aaaactgatc gaaaaatcag ggaaaacatc tatcaatgag aaaattgcta
     6961 ctgcacgaaa caaaggagct atcggcctga ttttacttac aaaccccaag aatcatctct
     7021 tgttgaaacc tcaggggcac acttgggcag cccttttgaa aagagcaaaa gaagattccg
     7081 atccgctgat tttgaaattt cacaaggaat catttactgc catacatgct ggtgaggaaa
     7141 cgatgaaatt attgtttggc agcaaagaaa acctcatcaa gattcaagaa aaaatcgaca
     7201 agaattttca accttattct tttgaaattc ccggatttac agcatccatt gcaacaagtc
     7261 ttcacaaacg ttttataaaa gccgacaacg tcattggaat tctcgaaggc aacaacccaa
     7321 aattgaggtc gcagtatctc atcattgggg ctcattacga tcatattgga acaatgaaat
     7381 ccgaaggaac tgataattcg gatactattt tcaatggtgc cgacgataat gcttcgggta
     7441 cggccgccgt actggctatc gccagagctt ttgcaaaagc cgaaaaattg ccagaaagaa
     7501 gtgtggtttt tgtgtttttt gctggtgaag aattaggatt gcttggctcg cgtagttttg
     7561 tcctcgaccc tcctctcccg ttagacagca tcattgccat gcttaatttc gatatggtat
     7621 gccgcggtgg aagcgatacg ctatatattt ctcccgaatc caccaattca gccttatatt
     7681 taatggctaa agaaaaagcc atggcagaag gattcaaaac ggtacaacaa aacatgattt
     7741 catttggtgg aagcgatcac cagccatttc tggataaaaa tatacccgca ttgcattttt
     7801 tcactggact tcatgcagat tatcatcaag taagcgataa tcccgaccgc tgcgattcgg
     7861 ttaaagcttc caaagtggct cggctcggtt ggaaacttgc atgggaaatt gccaattcaa
     7921 atattacctt tgcaaatcat tattcagaaa taaaaaatta aaggtccaaa atcattcgaa
     7981 aacttatctt tcattagaat taaaacaaca ccctcgattc atgaattgtt tcacacgatc
     8041 ttttcacata aaacctaaaa tttatgaaac accaacatct tgctctgctt ctattgtttc
     8101 ttagtcctct gatgggtatt gcccaaaaga aatacaatcc cgaggtcaaa ctcaacaaag
     8161 gcgattcaat aacctatcta attcaaacca atgtcgatat gactcaaagc atgatgggac
     8221 aggaaatgaa aatgaacgtt tccactcagt cgaaaaacat cattgccgta agtgatatcg
     8281 ataaggataa atattaccat ttaagccagt taagcaaaga aatgacggtt aaaatgaaaa
     8341 tggatcagat ggatagcacc atgctcgtcc ccgatattac caatcttcca gtattagtaa
     8401 tgagtaaaaa gggaaccatt ttacgcaaat cggtttcgac caatgaaaaa ggtcaaacaa
     8461 tgggtaatga ttttggagat gttacagcca gttcgccttt tattgaattt cccgacagaa
     8521 aaatttcgga aggcgaaagt tgggaaacgc ttcaaaatga taccgtgaac tttatgggtg
     8581 gcgatttgat taccaactca aaaaccgtat atactcttat tggaaccgat gaaaaagagg
     8641 gaatgaaatg cttgaagatc aatcataaaa caaccgccga caatgaaggg acaactacga
     8701 tgcaaggaat ggattttttg atagaaggaa ctaccatcat ggaaggaaca acatggattg
     8761 atttaaaaac ggccatacca gtttttatag atggcacggc cgaaagtcag atgacacttt
     8821 ta
//
LOCUS       DAOORU010000100         8752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_9928_length_8752_cov_5.539726, whole genome shotgun sequence.
ACCESSION   DAOORU010000100 DAOORU010000000
VERSION     DAOORU010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8752
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9928_length_8752_cov_5.539726"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..771)
                     /locus_tag="PLF20_07125"
                     /pseudo
     CDS             complement(1..771)
                     /locus_tag="PLF20_07125"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
     gene            892..2517
                     /gene="lnt"
                     /locus_tag="PLF20_07130"
     CDS             892..2517
                     /gene="lnt"
                     /locus_tag="PLF20_07130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOX80806.1"
                     /translation="MQLKKWHLALLALLAGSLLALSWPSRGFPGFLFVAWVPLFFMEQ
                     YFLDCKDKNSSWSLFGWAFLAFLIWNTLTTWWIWNSTKIGSIAAVLLNSFFMAVVFDL
                     YHITRRKLWQGQQFGWLILFYWPAWEYLHLNWDFSWPWLSLGNGFASYIKWIQWYEYT
                     GILGGTFWVLVSNLLIFILLKNIFTHTISPFRISILGIAIILWISVPLLISFHKYNTF
                     TDQGPATGVVIVQPNLDPWNEQFEIPVEEVFRRSIDLAIPFLDSSVDFLVAPESLLQE
                     NIWQNHPWNAKSIQLLRYFVAQHPDLTVLMGASTFKMYEPGEKLSPTVRKFTDGSGYY
                     DAYNTALVIDTGRELGYYHKSKLVPGPEKLPFPRLTRPIQEVVLNFGGTTGSLGISSE
                     RTVFTHRRTGARFNAAICYESVYGEFMTGFIRNGSQLIFVMTNDGWWGNTEGHRQHLT
                     FSSLRAIETRRCIGRAANTGISCFINQKGDILQSIPYWTKGAIKDQLHLNDNLTFYVK
                     YGDYIGRISALVTILFLFLVFCYNLMKRGKSKF"
     gene            complement(2535..3773)
                     /locus_tag="PLF20_07135"
     CDS             complement(2535..3773)
                     /locus_tag="PLF20_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX80807.1"
                     /translation="MLIIIKLIRESYLMAAAEISVNKTRTFLSLLGITIGIFCVVSVF
                     TVFDSMESTIKSSIESLGNNVVYIQKWPWLFGDDYPWWKYVNRPETKLEELKKLQERS
                     QTAEAMAFLTGTNRTVKSLKHSLKNVTVLAVSYDFFKLRNMEIGEGRYFSPLESNNGR
                     NVTILGNAIAQKLFDGEPAVGRQIKIYGLNTEVVGVLAKEGNNMGESFDEEVIVPVLF
                     AANFLNIDRDGSIAVIAKEGIAKQELIDELTGIMRSLRKLSPGMEDNFSINEISTIST
                     RFDQFFRIMAVIGWVIGGFSLLVGGFGIANIMFVSVKERTNMIGIQKALGAKNYFILL
                     QFLFEAVFLSLLGGILGLFIIFIFTLISTYAFDFRLILTFANIILGVTVSIIIGLIAG
                     IIPSYSAARMDPVEAMRSTF"
     gene            3965..5479
                     /gene="purH"
                     /locus_tag="PLF20_07140"
     CDS             3965..5479
                     /gene="purH"
                     /locus_tag="PLF20_07140"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOX80808.1"
                     /translation="MRIHRALISAFHKEILLPWAETLFSHGVELLATDGSYRFLKDKG
                     FEVQSIESLTGFPSIFSGRLKTLHPTIMGGILYQRDHPDDLNEAKLYGIPAIDLVIVD
                     LYPFEQTVASTNHQEEIIENIDIGGISLIRAAAKNFKSVAVVADNASMNEALEWYRKG
                     DGSFTEEQRMILATRAFAVSSRYDAAILSYFSNQTGNHYLRITEDHHKILRYGENPHQ
                     KGIYFGRSSSYFAQLHGKEISYNNLLDIDTALSLISEFTEPTIAIIKHGNSCGIASHA
                     ELMTALEMTLRGDPVSAFGGVIVSNSTIDKETVVKINSLFFEVILAPGFENEALQMLK
                     SRKNRVILQTTGKSLPDKLYRSAAGGFLMQDRDSFPDDPRQWECKTRVEPTPEQTEDL
                     LFANKVVKHLKSNAIALVKNKQLIGMGAGQTSRVDALRYAIEKSHNFGFDLEGAVMAS
                     DAFFPFADSVELAHKAGITAIIQPGGSIKDQESIDYCDQHNMAMVFTGIRHFKH"
     gene            5495..6517
                     /locus_tag="PLF20_07145"
     CDS             5495..6517
                     /locus_tag="PLF20_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOX80809.1"
                     /translation="MGLFSFLSREIAMDLGTANTIIIHNDRVVVDEPSIVAIERSTGK
                     VIAVGKRAQMMHGKTHENIKTIRPLRDGVIADFQAAEIMMREMIRMIPENHSIFPQSL
                     RMVISIPSGITEVEERAVKDSAEQAGAKEIRLIHEPMAAAIGIGIDVLEPTGNMIVDI
                     GGGTCEIAVIALGGIVTNESIHVAGDDFNDDIEEYMKKQHNINIGERTAERVKIEVGA
                     AITDLENPPADYAVHGRDMLTGIPKEIKVNYVEIAQCLDKSISKIEAAVLHALEMTPP
                     ELSADIYRTGIYLAGGGGMLRGLDKRITQKTKLPVHVAEDPLRAVARGSGIALKNFDK
                     FTFLIK"
     gene            6557..7402
                     /gene="mreC"
                     /locus_tag="PLF20_07150"
     CDS             6557..7402
                     /gene="mreC"
                     /locus_tag="PLF20_07150"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOX80810.1"
                     /translation="MNNILRFIARYHFFSLFLLLEIISFLLIFSFNYYPQAVYFKLAT
                     SFNGKVREINASIANFYKLKSTNRQLAIENSILRSSLPSSFLAVDTNVFWHHDTLYRQ
                     RYKYITAHVIGNSLGKRNNYIMIDKGSREGVGIDMGVIGPQGVVGIVVTVSDHFASIM
                     PVLHSATMISAKLKKNNELTSVIWKGGNPFKASLINLPGQVQISKEDSVVTSGYSLIF
                     PEGILIGTIDHFHLNTDDNYYDADILLSTRFGSLSYVYVVQNLLRDEQLQLLKKQDEI
                     ISEEN"
     gene            7549..>8752
                     /locus_tag="PLF20_07155"
     CDS             7549..>8752
                     /locus_tag="PLF20_07155"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009040806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOX80811.1"
                     /translation="MTKKVFLTFFLACGFILGSYACTNYIITKGASVDGSVMISYSAD
                     SHVLYGELYHWDAATWPAGTMLEVYEWDTGKYLGKIEQALQTYNVIGNMNEYQVSIGE
                     TTYGGREELWHQPGSIIDYGNMIYIALQRSRTAREAIKVMVELSEKYGYVSEGESFSV
                     ADAEEAWILEVIGKGKEIRIDASGLRKESKEFQRTLKPFVDKVFYSDHDFLKKLEEIA
                     GKNFAEEHGKRLIEEYQKGIKGMVWVALRIPDGTVSGHANQARITTFPLANGTTSITS
                     QNLDKILDPQVECAYSYDVISFARQRGYITDAITDEKFSFSDTYAPLNFEAARFSEIR
                     VWTMFNKVAPGMSKYWEYVKGYIEFDPVTGYGSNRMPLWVKPQKKVRVRDMMDFMRDH
                     LEGTELDMS"
BASE COUNT         2610 a         1805 c         1788 g         2549 t
ORIGIN      
        1 ttgatgaaaa gttttggaaa aatttttagg gtgacgcatt gtcgaagtca ggagatcaca
       61 accagctagg gcaattacct gatctatatt tcgaaaactg gctcccatga tttcggtagg
      121 atacccaaat ttcatataat aatggaagat gtcacaaaca ctttttactc caggatcctc
      181 ttcgattgga acatacgtaa gccctttctc ttttttgtac cagtcgagga tacggccgac
      241 aaacggagaa ataagggtca catgagcttc agcgcatgcc actgcctgaa ttttcgagaa
      301 aagtaaggtg agattgcaat gaatgccatc cttctcaagg gcttcggcag ctcttatccc
      361 ttcccatgta ctcgcaatct tgataagaat acgttttcta ttcattcccg cttcttcata
      421 tagatcaatc aagtgccttg ccttggaaat gcttctttgt gtatcaaaag ataatcgggc
      481 atcaacttcg gtacttactc ggccgggtac aatttttaaa atctccagtc aaaacaatag
      541 ggctatttca tccagacaaa tactcaattg atgttttgga tcaaacgact ttcgtttggc
      601 tatagaaaca gctttttcga ccagataacg gtacttttcc tgacaggctg ccacatagat
      661 caatgaagga tttgttgttg catctacagg gcgataagcc aaaatagatt caaaatcacc
      721 tatatcggct actactttgc tgtattgtcg aagttgctca aggctgttca ttgcactttt
      781 aaccaaaatt gagttataat tcgttgcaaa aataatgaac ctctttcgat tacttcgaat
      841 cattcctatc agtatggaaa accccatatc tttgaaccca aaaatagaag gatgcaactg
      901 aaaaaatggc acttggctct gctggctctg ctggcgggta gtcttcttgc actttcatgg
      961 ccatcgaggg gattcccggg atttctgttt gtggcatggg ttcctttatt ttttatggaa
     1021 caatattttc ttgattgtaa agataaaaat agcagctgga gcttatttgg ctgggcattt
     1081 ttggcctttc ttatttggaa taccctcact acatggtgga tttggaattc aaccaaaata
     1141 ggaagcattg cagcagttct actcaacagc ttttttatgg ccgttgtatt cgatctttat
     1201 catattacac gtcggaaatt atggcaaggg caacagtttg gctggctcat cctgttttac
     1261 tggcctgcat gggaatacct tcacttaaat tgggattttt cgtggccatg gcttagtctt
     1321 ggtaatggtt ttgcttctta tataaaatgg atacaatggt atgaatatac gggtatattg
     1381 gggggaacct tctgggtcct ggtctccaat ttattgattt ttattctgct gaaaaatatt
     1441 tttacccaca ctatctcgcc atttaggata agtatattgg gtattgcaat catactttgg
     1501 atttctgttc cgctgctgat ttcttttcac aaatataata cctttacgga tcaaggccct
     1561 gctaccggag tggttattgt tcagccaaat ttggatccat ggaatgaaca attcgagata
     1621 ccggtcgaag aagtattccg tcgcagcatt gatttagcca ttcccttcct cgattcgagt
     1681 gttgattttc tggtagcacc cgaatctctc ctacaggaaa acatctggca aaaccatcca
     1741 tggaatgcca agagcataca attattaaga tactttgttg cccaacatcc cgatctcaca
     1801 gtattgatgg gtgcttctac attcaagatg tatgaacccg gagaaaagct ttctcctaca
     1861 gtaagaaagt ttactgacgg aagcggatat tatgatgctt acaatactgc gcttgtgatc
     1921 gataccggta gagagctcgg atattatcac aaatcgaaat tagtccctgg ccccgagaaa
     1981 cttccatttc cacggcttac tcggcccatc caggaagtcg tgttgaattt tggagggact
     2041 actggaagtc tgggtatttc ttccgagcgt acagtattta cacatcggcg taccggagca
     2101 cgattcaatg ccgctatatg ttacgaatcg gtttacggag aatttatgac aggatttatc
     2161 cgaaatggtt ctcagctcat atttgtgatg acaaatgatg gttggtgggg caataccgaa
     2221 ggacaccgcc agcatcttac cttttcatct cttcgtgcca tcgagaccag acgctgcata
     2281 ggaagagccg ccaatacagg aatctcgtgt ttcattaatc aaaaagggga tattctgcaa
     2341 tccattccat attggaccaa aggcgccatt aaggatcaac tccatttgaa cgacaatctt
     2401 accttttacg taaaatacgg agattacatt ggacgcatct cggctttggt tacgattttg
     2461 tttttattcc ttgttttttg ttataatttg atgaagaggg gcaaatcaaa attctgagtt
     2521 tctttaagca aatctcaaaa tgtcgatcgc atagcttcta caggatccat gcgagccgca
     2581 gaataagaag gaattattcc ggctatcaat cctataataa tcgaaaccgt aactcccaga
     2641 ataatattgg caaatgtcag aatcagccga aaatcgaagg cataagtact gatcaaggta
     2701 aagatgaata tgatgaatag gcctaaaata ccgcccagca agcttaaaaa aacagcttca
     2761 aataaaaact gcaggagaat gaaataattc ttggcaccca gagctttttg aatgccaatc
     2821 atattggttc gttcttttac cgatacaaac ataatattgg caattccaaa tccacccact
     2881 agcaatgaaa aacccccaat aacccatccg atgactgcca ttatgcggaa aaattggtcg
     2941 aatcgggtac taatggtact gatttcgtta atggaaaaat tatcttccat tcctggcgat
     3001 aatttgcgta gcgagcgcat aataccagta agctcatcga tgagctcctg ttttgcaatt
     3061 ccttctttag caatgactgc aatggaacca tcgcgatcga tattgagaaa atttgcagca
     3121 aaaagaacgg gcacgatcac ctcctcatcg aagctttctc ccatattatt cccttccttt
     3181 gcaaggactc ctacgacttc ggtatttaat ccgtaaatct ttatttgtcg cccaactgcc
     3241 ggttctccat caaacaactt ttgtgctatg gcatttccta aaatcgtcac atttcttcca
     3301 ttattagact caagaggcga aaaataacgg ccttcgccaa tttccatatt gcgcagcttg
     3361 aaaaaatcat acgaaactgc caaaaccgtc acatttttca aactatgttt caaacttttt
     3421 accgtcctgt ttgtaccagt taaaaaggcc attgcttcgg cagtttgcga ccgctcctgc
     3481 agcttcttca attcttccaa tttggtttcg ggcctgttca cgtatttcca ccagggataa
     3541 tcatctccaa ataaccaggg ccatttctga atgtaaacca cattatttcc caaagattct
     3601 atgctcgatt taattgtgct ttccatcgaa tcgaatacag taaaaaccga aaccacacaa
     3661 aagatcccta tggtaatccc cagcaacgat aaaaaggtgc gcgtcttatt tacacttatt
     3721 tcagctgctg ccatcaggta gctttccctg attagcttaa tgatgatcaa catataaaaa
     3781 ttctattgtt atgattatgc aaacctacat gaaaagagtt tatctcaatt tgaaaaggac
     3841 aaattaattt gcatttcgaa tggaattgtt tgccttatga atgcattttg gttaaaattg
     3901 cagccgcatt ctcatttttt ataaatgggt ctatttaagt tgttttcgtg aaattttatc
     3961 ccttatgaga attcatagag ccttaatttc ggcattccac aaagaaattc tccttccgtg
     4021 ggccgaaact ctcttttccc atggagtcga gcttctggca acggatggat catatcgctt
     4081 cttgaaagat aaagggtttg aagttcaaag tatagaatct ttaacaggat tcccttccat
     4141 ttttagtggc aggctcaaga cgctgcatcc cacgataatg ggaggcatac tatatcagcg
     4201 tgatcatccc gacgacctga acgaagccaa attatatggt attcctgcca ttgaccttgt
     4261 aatagtggat ttgtatccct ttgaacaaac cgtagcaagc acaaatcatc aggaagaaat
     4321 tatcgaaaat attgatattg ggggcatttc gctaattcga gcggctgcca agaattttaa
     4381 atctgtagcc gtagtggccg ataacgcaag catgaatgaa gccctcgaat ggtatcgaaa
     4441 aggggatggc tcttttactg aggagcaacg catgatactt gccacacgtg cctttgcagt
     4501 cagctcacgc tatgatgcgg ctatcctatc ctatttcagc aatcagaccg ggaaccacta
     4561 cctcagaatt acagaagatc atcacaaaat attgcgttat ggtgaaaacc cacatcaaaa
     4621 ggggatttac tttggcagat cgagttcgta ttttgctcaa cttcacggaa aagaaatttc
     4681 ttacaacaat ttactcgata tagatacggc attaagtctg atatctgaat tcaccgaacc
     4741 caccattgct attatcaaac acggtaattc atgcggaata gcttctcatg cagaattaat
     4801 gaccgctttg gaaatgacac ttcggggaga tccagtatcg gcctttggtg gtgtgattgt
     4861 cagcaatagt accatagata aagaaacagt ggtaaaaatt aattccttgt tttttgaagt
     4921 tattcttgct ccgggatttg aaaatgaggc tctccagatg ttgaaatctc gtaaaaacag
     4981 agtaattttg caaactaccg gcaaaagttt acccgataag ctataccgtt cggctgcagg
     5041 aggtttttta atgcaagaca gagatagctt ccccgacgat cctcgacaat gggaatgcaa
     5101 gactcgagtc gagcctactc cggaacaaac agaagacctg ttgtttgcca acaaggtagt
     5161 aaagcatttg aagtcgaatg ccatagccct tgtaaaaaac aaacaactta tcggtatggg
     5221 agccgggcaa acttcgagag tagatgctct gaggtatgct atagaaaaga gccataattt
     5281 tggcttcgac cttgagggag cagtgatggc ctccgatgcc tttttccctt tcgccgattc
     5341 agttgaattg gctcataaag cagggatcac tgccattatc cagcccgggg gttctattaa
     5401 agaccaggag agcattgatt actgtgacca gcataatatg gccatggtat ttacaggcat
     5461 tcgacatttt aaacattaac atataaatcg ttttatgggc cttttttcat ttttaagcag
     5521 ggaaatcgca atggatttgg gtacggccaa taccatcatt atacataacg atcgagtagt
     5581 agtagacgaa ccaagcattg tagccattga acgctctacg ggcaaggtga ttgccgttgg
     5641 caagcgagcc caaatgatgc atgggaaaac ccatgaaaac attaaaacca tacgtccatt
     5701 acgcgatgga gtaattgccg attttcaggc cgccgaaatc atgatgcgtg aaatgatacg
     5761 aatgataccc gaaaaccata gtatctttcc tcaatcactc agaatggtca tttcgatacc
     5821 ttcgggtatt accgaagttg aagaacgtgc ggtaaaagat tcagccgagc aggcaggtgc
     5881 aaaagaaatc cgtctgattc atgaacctat ggccgctgct attggaattg gtatcgacgt
     5941 tctagaacct accggaaaca tgattgttga tattggaggt ggtacttgtg aaattgctgt
     6001 tatagccctt ggtggaatcg taaccaatga atctattcac gtagccggag atgattttaa
     6061 cgacgatata gaagagtaca tgaagaaaca gcataatatc aacatagggg aacgtactgc
     6121 cgaaagagtg aaaattgaag tgggagctgc gatcaccgat ttggaaaatc cccctgccga
     6181 ctatgcggtt catggccgcg acatgcttac cggaatccct aaggaaataa aagtaaatta
     6241 tgtcgaaatt gcccaatgcc tcgataaatc aattagtaaa attgaggctg ccgttctcca
     6301 tgcactcgaa atgacacctc ctgagctttc ggcagatatc tatcgtacag gtatctatct
     6361 ggcgggtggc ggagggatgc ttcgaggcct cgacaaaaga atcacacaaa aaaccaaatt
     6421 acccgttcat gtagccgaag atcctctgcg cgccgtcgcc cgaggctcag gaatagctct
     6481 caagaatttc gacaagttta cattccttat taaataatat taggagaaat atttttacga
     6541 aaccggccga aagattatga ataacatcct gaggtttata gctcgatatc atttcttttc
     6601 cctcttttta ttactcgaga taatatcctt tttactcata ttttctttca attattatcc
     6661 tcaggctgtt tatttcaaac tagctacttc ttttaatgga aaagttcgag aaatcaatgc
     6721 ttccattgcc aatttctata aattaaaatc tacaaaccga cagcttgcca tcgaaaactc
     6781 catcctgcgc agctcccttc cttcgagctt tctggctgtc gataccaacg tcttctggca
     6841 tcatgatacc ctttacaggc aaagatacaa gtacatcaca gcccatgtca tcggtaacag
     6901 ccttggcaaa cgcaacaatt acatcatgat tgacaaggga agtagagagg gagttggcat
     6961 cgacatgggg gtcataggac ctcaaggagt agtaggaatt gttgtgactg tttccgacca
     7021 ttttgcttct attatgcctg tgcttcacag tgctactatg attagcgcaa aactgaaaaa
     7081 aaataatgaa cttacttcgg ttatctggaa aggagggaat ccattcaaag ccagcctgat
     7141 caacctaccg ggacaagttc aaatttcaaa agaagacagc gtggttacta gcggctattc
     7201 tttgattttc cccgaaggta tccttatcgg aaccattgat cattttcatc tgaataccga
     7261 cgataattat tatgatgccg atatattact gagtacgaga ttcggcagtc tttcctatgt
     7321 ttatgtggtt caaaatcttc tgagagacga acaactgcaa ctgctgaaaa aacaagatga
     7381 aattatttct gaagaaaatt aatctcattg gtcaattatt taattatcag caatttattt
     7441 gacaacaggg ctttatttgc tcaaggggtg gggggtatgt aaaaaataat ctataatttt
     7501 aggccatcaa actaaccttt tctttattta atttataaat tgatttttat gactaaaaaa
     7561 gtatttctta cattcttttt agcctgtggt tttattttgg gcagttatgc atgtaccaat
     7621 tatatcatta ccaaaggcgc atcagttgat ggaagtgtaa tgattagtta ttcggccgat
     7681 tcgcatgttc tttatggaga actatatcat tgggatgctg ctacctggcc tgcaggaacc
     7741 atgcttgagg tatacgaatg ggatacgggc aagtatctgg gtaaaattga acaagctctt
     7801 caaacctaca atgtgatcgg gaatatgaac gaatatcagg tttctattgg tgaaaccact
     7861 tacggaggcc gtgaagaact gtggcatcag ccgggttcta taatagatta tggcaacatg
     7921 atctatatag ctttgcaacg atctcgcacg gcgcgtgaag ctataaaagt catggtcgaa
     7981 ctatccgaaa aatatggtta cgtgagcgaa ggtgaatcat tctcggttgc cgacgcggag
     8041 gaagcatgga tattagaagt tattggaaaa gggaaagaaa ttcgcatcga tgccagtggc
     8101 ttacgcaaag aaagcaagga atttcagaga accctaaaac ccttcgtgga taaagtattt
     8161 tatagtgacc atgattttct gaaaaaattg gaagaaatag ctgggaaaaa ttttgcagaa
     8221 gaacatggca agcgccttat cgaagagtac caaaagggaa taaaaggaat ggtatgggta
     8281 gccttgagaa tacccgatgg tacagtttcg ggccatgcca atcaggctcg tataactacc
     8341 ttcccactgg ccaatggaac tacctccata acttctcaga atctcgataa gattttagac
     8401 cctcaggttg aatgtgctta ttcatacgat gtgatatctt ttgcccgtca gcgtggatat
     8461 atcaccgatg ctataactga cgaaaaattc agctttagtg atacttatgc ccctctcaac
     8521 ttcgaagcag cacgattttc tgagatcaga gtctggacga tgttcaacaa agtagctccc
     8581 ggcatgagta aatattggga atatgttaaa ggctatatag aattcgatcc agttacgggt
     8641 tatgggagca atcgtatgcc tctttgggtt aaaccccaaa aaaaggtcag agtaagagat
     8701 atgatggatt ttatgcgcga ccatctggaa ggcaccgagc tcgatatgag ca
//
LOCUS       DAOORU010000101         8533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_10257_length_8533_cov_5.509436, whole genome shotgun sequence.
ACCESSION   DAOORU010000101 DAOORU010000000
VERSION     DAOORU010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10257_length_8533_cov_5.509436"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="PLF20_07160"
     CDS             <1..917
                     /locus_tag="PLF20_07160"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOX80812.1"
                     /translation="YYTITDVDLNDVENVIASPDLISYAEERGWYDQAQDGDFNFRKS
                     YGNDDNLNHPVNVMRMWCALNKLSGQTYAVEDEFPWIIHPKKRISVENLMDLLGTHQQ
                     GEAMKEKEEAEGSWHPHYEGSNSICAGHTRYGFVAQLQSGYPVDFGCVMWLAPYRPCT
                     QTFIPLFCGMLSAPENMQSRDLKDARRLHFESLAMNDLPSSHAYLSFKAYSDMVETNY
                     STLIPQISKEIEAQTMENLRGYQAKLLDWTAAYKEKPKELRKQITEFSHTAFSLSLGR
                     VRSFLSKQAPQQSESFIQSPSLQETEVK"
     gene            1434..5186
                     /gene="purL"
                     /locus_tag="PLF20_07165"
                     /gene_synonym="purI"
     CDS             1434..5186
                     /gene="purL"
                     /locus_tag="PLF20_07165"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOX80813.1"
                     /translation="MFADKTFVSEFQYQSRLKMILIFRSRDFSKVYALESSFSLTSSD
                     IQKLRWLFHEAEFIEEGYLKGRFIGPRREMITPFSTNAVEITQIMGISGIRRIEEFRI
                     MKPGDDYDRMLEAIYENLHQDIFRIDRLPAPVEFIEDIAAYNEKAGLALRKEEIEYLE
                     EISRLMGRKLTDSEVFGFSQVNSEHCRHKIFNGTFIIDGQRQAYSLFELIKATTQKNP
                     NRVVSAYKDNVAFLSGPQIIQFSPKNPTFPDYFSVEPIDSVISLKAETHNFPTTVEPF
                     NGAATGSGGEIRDRMAGGQGSIPLAGTAIYMTSYPRTSLQRPWESFLPEREWLYHSPA
                     QILIKASNGASDYGNKFGQPLICGSLLTFEHKSGSITYGYDKVVMLAGGVGYANATHS
                     QKRHPEPGDKIVVMGGDNYCIGMGGGAVSSVDTGRYENSIELNAIQRSNPEMQKRVAN
                     ALRAMCEQTNNPVISVHDHGAGGHLNALSELVDDEGGIIYLDQLPVGDPTLSAKEIIG
                     NESQERMGLLMKQEDIAFLEKVAQRERAPMYEVGYITGDDCFVVEDSRNHLRPIDLRL
                     EYLFGKPPHLVLEDNSKPHEHELLEYDPSLWYDYLLQVLQCEAVACKDWLTNKVDRSV
                     TGRIAHQQTTGPLQLPLNNLGVVALDYDGNLGIATSIGHAPIAGLIQPDAASRLSVAE
                     ALTNLIWAPLAYGLKGVSLSANWMWPCYNDGEFARLYRAVEALSQFAIALGINIPTGK
                     DSLSMTQKYPDGSKVLSPGTVIVSAAAEVSNIRNVVSSVICPVFPSFLIYIDFSDVEF
                     HLGGSTFAQVLNSVGNTSPDVKDTVYLVRAFHAVQELVKHKYVLAGHDVSAGGLVVTL
                     LEMHFSQPDVGSRLQLDALPEPDLIRLLFSEKPAVVVQTDKLPEVSAILDKNSLRWAI
                     IGQLEPKRELRMLHCNREYVLDIDELRNEWFRSSYLLDRLQSGNIKARERFENYARTE
                     RKMRFPDEFTGCMSDLGIDSKRKSPSGIKAAIIREKGSNGDREMAYALWLAGFDVKDV
                     HVTDLVSGRETLDEVQMIVFVGGFSNSDVLGSAKGWAGAFLYNPKAKASLEHFYRRHD
                     TLSLGVCNGCQLMAELNLIYPAHEQRFEMLPNESQKFESTFLTVKIPRNRSVMLSSLE
                     GSELGIWVAHAEGRFYFPYQENRYQITAKYSYDFFPGNPNGSMYDAAAVVSEDGRHLA
                     IMPHLERSIFPWQWGYYPHHRKEDEISPWMMAFVNARKWIKNKNKG"
     gene            5255..5965
                     /gene="deoD"
                     /locus_tag="PLF20_07170"
     CDS             5255..5965
                     /gene="deoD"
                     /locus_tag="PLF20_07170"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOX80814.1"
                     /translation="MGIHLDARVGEIAESVLLPGDPLRAKFLAEKYFDNPVCHNQIRG
                     MFGYTGYVNGKKISVQGTGMGVPSISIYVTELIQTYGCKNLVRIGTCGAIQPELKLGD
                     TVLAITASTDSHVNTLTFGGREYAPHADFELLKMAYNSGKALGHQVKVGSVFTTDSFF
                     VDDLEKEYEIWRRHGVLGVEMETSALYTLAARFRCRALSILTVSDQLVTGERATAQER
                     LTAFTAMMEIALACLKSL"
     gene            complement(6053..7171)
                     /gene="dnaN"
                     /locus_tag="PLF20_07175"
     CDS             complement(6053..7171)
                     /gene="dnaN"
                     /locus_tag="PLF20_07175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOX80815.1"
                     /translation="MKFIISSTNLLKSLQTISGVVTTSNTLPILEYFLFKTKENDLEI
                     TASDQETTMSITVTPIMMEDGGAIAIPSKILLDTLKTYHDTPLTFLTDPSTFAVEIIA
                     DNAKFKLSGQDPQDFPEIPELINPATVTVNGEILVRGISKTIFATGTDELRPEMTGVL
                     FEFTEDKATLAATDAHKIIRYRRPDVHSSETMSFIMPKKPLNQLKNTVPSDETPVLIE
                     YNLKNARYTFNNVVLTSTLIEGKFPNYEAVIPRDNPNILIIDRIALMTSIRRVSLFGN
                     QSTHLIRFRISGQMISLSAEDYDFQNEASEVLSCAYDGEDIEIGFNSKFLLEMLSNID
                     TPNIRIETSAPNRAGVIFPETDENEVEDLLMLIMPVMI"
     gene            complement(7415..8200)
                     /locus_tag="PLF20_07180"
     CDS             complement(7415..8200)
                     /locus_tag="PLF20_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80816.1"
                     /translation="MKLLEKPILIVQLIFFLFSSVIALSQGINQIDAQGRKQGVWRKS
                     YENGQIRYEGNFQNDQPVGEFRHYHPNGKLKAIIIHTGDGHSSAAEFYNENEILMARG
                     FFYDEWKDKTWEYFNQKNGKLISIENYQQGIPHGEWIVYYKEDTVVSENFTYDHGQKE
                     GPWKQFFADGKLKLDASYLHHQLDGPYSLYSPEGKEVVKGFYQNGKKMGEWEFYTNDG
                     QLKRIEWYDQNEKIKEEIFIPEEPIKDVPLDPLLDPENQIRIP"
     gene            8311..>8533
                     /locus_tag="PLF20_07185"
     CDS             8311..>8533
                     /locus_tag="PLF20_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HOX80817.1"
                     /translation="MENYPSKYLEAAVNELSKLPGIGRKTALRLCLYLLDQPQAFAQN
                     LGEALIRLRENIRYCEQCHNISDEPVCRIC"
BASE COUNT         2399 a         1574 c         1973 g         2587 t
ORIGIN      
        1 actactatac catcaccgat gtagatttga atgatgtgga aaatgtaata gccagtcccg
       61 atcttatcag ttatgctgaa gaaaggggat ggtacgatca ggctcaagat ggggatttta
      121 attttcggaa atcttatggt aacgacgaca acctgaatca tcccgtgaat gtcatgcgaa
      181 tgtggtgcgc cttgaataaa ttatcaggac agacctatgc ggtggaagat gaatttccat
      241 ggattattca ccctaagaaa agaatcagtg tagaaaatct gatggattta ttgggaacgc
      301 atcagcaggg agaagccatg aaagaaaagg aagaagcaga aggaagttgg catccgcatt
      361 acgaaggttc aaactccatt tgtgccgggc atacacgtta tggctttgtg gctcagctcc
      421 aaagtggtta ccctgttgat tttggctgtg taatgtggtt agctccttat cgaccatgta
      481 cacaaacctt tataccttta ttctgtggaa tgttgtcggc acctgaaaat atgcagtcga
      541 gagacttgaa agatgcgcga agattgcatt tcgaatcact tgcaatgaat gatttaccgt
      601 cgagtcatgc ttatttaagt ttcaaggctt atagtgatat ggtagaaacc aactattcta
      661 cattaattcc tcagatttcg aaagaaattg aggctcaaac catggaaaat ttgagaggat
      721 atcaagcaaa gcttcttgat tggacggctg cttataaaga gaagccaaag gaattgagaa
      781 agcaaattac tgaattcagt catactgctt tttcgctcag tctcgggagg gtaaggagtt
      841 ttctgtcaaa acaagcgcct caacagagcg agtcatttat tcaatctccg tcattgcagg
      901 aaacggaggt caaatgagta attttgttgt tcatttttgg atatgaatta aaaattcctg
      961 accatgataa ttatcagtcg cttttatttc aaaacagttt aaattccaga agaattcgag
     1021 tttctcctgc taaaggttat ggttttttta ttgaaatttc ttaacccttg agatatttga
     1081 attcaaattt gtcattagaa taaagattct attgctggtt atagatagag agaatgaaat
     1141 ataatgaggt ggattactgt ataatatagg attactgtat aatatattag agggaaagaa
     1201 ttcattctgg ttttgaattt agaataataa aagaaattac acagagtaaa tgaaagaatc
     1261 caaaatagca gagaacacga aattttattg acggtggcct tgataagggc ttcgacaagc
     1321 tcggctttat tcggccacct gcattcatcg attgcagggt aaactcctta gagcgttggt
     1381 ggcaactgcc tcttgaggtt tatattggat aatttgggtt aaaaaaggtc attatctttg
     1441 cggataaaac atttgtaagc gagttccaat atcaatcaag gcttaaaatg atattaattt
     1501 ttcgatcgag agatttctca aaggtttatg ccctggaatc ctcattttct ctcacttcga
     1561 gtgatattca aaaactcagg tggctttttc atgaagcaga atttattgag gaaggttatt
     1621 tgaaaggaag gtttattggg ccaaggcgtg agatgattac gccttttagt acgaatgccg
     1681 tcgaaattac tcaaattatg ggaatttcgg gtatccgtcg catagaagag ttcaggataa
     1741 tgaaaccagg tgatgattat gatcgcatgc tcgaagcaat ctatgaaaat cttcatcagg
     1801 atattttccg aatcgaccgc ttgcccgcgc ctgttgaatt tatcgaagat attgcagctt
     1861 ataacgaaaa ggccggactt gctcttcgta aggaagaaat tgaatatctt gaagaaatca
     1921 gtcggttgat gggacgaaaa ttgactgata gcgaagtgtt cggtttttcg caggtaaact
     1981 ccgagcactg ccggcacaaa atattcaatg gtacttttat catcgatggt caaagacaag
     2041 catattcttt attcgaactt atcaaagcca caacacaaaa aaatcctaat cgggttgttt
     2101 cggcttacaa ggataatgtg gcctttctat caggcccaca gatcatacaa ttttcaccta
     2161 aaaatcccac ttttcctgat tatttctcag tcgagcccat tgattcggtc atttcgttga
     2221 aagccgaaac ccataatttt ccgactacag tcgagccatt caatggggca gctacgggta
     2281 gtggaggcga gataagagac agaatggctg gtggccaggg cagtattcct ctggcaggaa
     2341 ctgctattta tatgacttcc tatccacgta cttcactcca acggccatgg gaatcattcc
     2401 ttcctgaacg ggaatggttg tatcattctc cggctcaaat actcatcaaa gcctctaacg
     2461 gtgcaagcga ttacgggaat aaatttggtc agccattaat ttgtggttct ttgctaacat
     2521 tcgagcataa aagtggcagt ataacttatg gttacgataa ggtagtgatg cttgccgggg
     2581 gggttggtta tgccaatgca acccattcgc agaaaaggca tcccgagccg ggagataaaa
     2641 tcgtggtgat gggaggcgat aattattgta tcggaatggg cggtggggct gtgtcaagcg
     2701 tagatacggg ccgctatgaa aattctatcg aactgaatgc catccaacga tcgaatccag
     2761 aaatgcaaaa acgagttgcc aatgcgctca gggctatgtg cgaacaaacc aataatcctg
     2821 tcatttcagt tcatgaccac ggagccggag gacacctgaa tgcactttcg gaactggtgg
     2881 atgacgaagg gggaatcatc tacctcgatc agctccctgt tggcgatcct accttatcgg
     2941 ccaaggaaat cattggaaat gaatcacaag aacgcatggg ccttttgatg aaacaagaag
     3001 atatcgcatt tttagagaaa gttgctcaaa gagaaagggc gcccatgtac gaggtgggct
     3061 atattacagg cgatgattgc tttgttgtag aagactctcg caatcatcta cgccccatcg
     3121 atctgcggct cgaatatctt ttcggcaaac ctcctcatct tgttttagaa gataatagca
     3181 agccccatga acatgaactt ctcgaatatg atccatcctt gtggtatgat tatctgttgc
     3241 aggttttgca atgtgaagcc gtagcttgta aagactggct taccaataag gtagatcgtt
     3301 cggtaacggg tcgtattgct catcagcaaa ccacggggcc tttacagctt cctttgaata
     3361 atttgggtgt cgtagcattg gattatgacg gcaatttggg tattgcaact tctattggcc
     3421 atgctcctat tgcagggctc atccagcctg atgccgcttc ccggctgtca gtagctgaag
     3481 cacttaccaa tttaatttgg gctcctttgg cttacggcct gaaaggtgtc tcactttcgg
     3541 ccaactggat gtggccttgc tataatgatg gagagtttgc ccgcctctat cgcgctgtcg
     3601 aagcattgag ccagtttgcc attgctctgg gaatcaatat tcctacgggg aaggattctc
     3661 tcagtatgac acaaaaatat cccgatggca gcaaggtgct atcgccaggt actgtcatcg
     3721 tttctgctgc tgctgaggtt agcaacattc gcaatgtagt ttcatccgtc atctgtccgg
     3781 tattcccttc ttttcttatc tatattgatt ttagcgatgt agaatttcat ttaggtggaa
     3841 gtacttttgc gcaagtattg aattcagtgg gaaacacatc tcccgatgtg aaagatacgg
     3901 tatatcttgt gcgcgctttt catgctgttc aggaactagt gaagcataag tatgttcttg
     3961 cgggtcacga tgtttcggct ggaggtcttg tcgttacctt gctcgaaatg catttcagtc
     4021 agccagatgt gggaagtcgg cttcaactcg atgcgcttcc cgaacccgat ctgattcgtc
     4081 ttttatttag tgagaaacct gcggtagtcg tacagaccga taaacttccc gaagtgagtg
     4141 caatcctcga taaaaattcg ttacgctggg caatcattgg ccagctcgag ccaaaaaggg
     4201 aactgagaat gcttcattgc aatcgagaat atgtattgga cattgatgaa ttgcgaaatg
     4261 aatggtttcg ctcgtcctat ttgctcgata ggctgcaaag tgggaatata aaagccagag
     4321 aaagattcga gaattatgca cgaacggaaa ggaaaatgag atttcccgat gagtttacag
     4381 gttgcatgtc cgatttgggt atcgattcca agcgtaaatc cccatcagga ataaaggctg
     4441 cgattatacg tgaaaagggt tcgaatgggg atcgtgaaat ggcgtatgcg ctttggctgg
     4501 ccggtttcga tgtaaaggat gttcatgtta ccgatttggt gagtggtcgt gaaaccctcg
     4561 atgaagtgca aatgattgta tttgtgggag gattttccaa cagtgatgtg ctgggttcgg
     4621 ctaagggctg ggcaggagct tttttgtaca atcccaaagc caaggcttcg cttgagcatt
     4681 tctatcgtcg ccatgatacg ctttcgcttg gtgtttgcaa tggttgccag ttgatggccg
     4741 aattgaatct catctatccc gcgcatgaac aaagattcga aatgttaccc aatgaatcgc
     4801 aaaagttcga gtcgactttc ttaactgtca aaattcctcg gaatcgttcg gtgatgttga
     4861 gttccttaga gggttcggag ttagggatat gggtagcaca tgccgaaggg cgtttttatt
     4921 tcccttacca agaaaaccgt tatcagataa ccgctaaata ttcgtatgac tttttcccgg
     4981 gcaatccgaa tggctcgatg tatgatgcag cagcagtagt atcggaagat ggacgtcatc
     5041 ttgccataat gcctcatctt gaacgttcca tctttccatg gcaatggggt tattaccctc
     5101 atcacaggaa ggaagacgaa ataagccctt ggatgatggc tttcgtgaat gcccgaaaat
     5161 ggattaaaaa taaaaacaag ggataatcct atacagtaca gataaaattt tgatgaaata
     5221 aaatcgaatt gttaatcaga taaatgaaga ttctatggga atacatttag atgcaagggt
     5281 aggtgagatt gccgaaagtg tcttattgcc cggtgatcca ttaagggcta aatttttagc
     5341 cgaaaaatat ttcgataatc cggtttgtca caatcagata agaggaatgt ttggctatac
     5401 aggttatgta aatggaaaaa agatttcggt acagggaaca ggaatgggag ttccttctat
     5461 aagtatatac gttacggagc ttatacagac ttatgggtgc aagaatttag ttcgcattgg
     5521 tacatgtggt gccattcagc ctgaactgaa attaggcgat actgtattag ccattactgc
     5581 aagtaccgat agtcatgtaa atactttgac ctttggaggg agggaatatg ctcctcatgc
     5641 tgatttcgaa ttgttgaaaa tggcttataa tagtggaaaa gcattagggc atcaagtgaa
     5701 ggttggaagt gtatttacaa cggattcttt ttttgtggat gatttggaga aggaatacga
     5761 gatttggcgc agacacggag ttttgggtgt agagatggaa accagtgcgc tttacacgct
     5821 ggcagcacgt tttcgctgta gagccttgtc gattttgacg gtcagtgatc agttggttac
     5881 gggcgaacgt gccacggctc aggaaagact tactgcattt acagctatga tggaaattgc
     5941 cttggcctgt cttaaaagct tataggaata cgaaaaaccc cttatcattt tttcgataag
     6001 gggctggaga attcagcagt ttgttattgc tgatgggaga gaggctaatt tcttagatca
     6061 ttacaggcat aatcaacatc agaaggtctt ctacttcatt ttcgtcggtt tcgggaaaga
     6121 taacacctgc gcggtttgga gccgacgttt cgattctgat gttgggtgta tcgatattgg
     6181 atagcatttc gagcagaaac ttggagttga atccaatttc gatgtcttcg ccatcataag
     6241 cacaggatag aacttccgag gcttcattct ggaaatcgta gtcttcagca cttagtgaga
     6301 tcatctgacc tgaaattcta aagcgaatca ggtgggtgct ttgattgcca aagagggaaa
     6361 cccttcgaat ggaagtcatt aaggctatac ggtcgatgat gagtatattg ggattgtcgc
     6421 gcgggatgac ggcttcataa ttgggaaatt tgccttcgat gagggtagaa gtaagtacta
     6481 cattattgaa ggtatatctg gcatttttca ggttatattc gatcagaacg ggagtttcgt
     6541 ccgaaggcac agtatttttc aattgattga gaggtttctt tggcatgata aacgacatgg
     6601 tttcggatga atggacatct gggcgcctgt atcgaatgat tttatgtgca tcggtagctg
     6661 ccagggtagc tttgtcttca gtaaactcaa ataatactcc cgtcatttcg gggcgtaatt
     6721 catcggttcc cgttgcaaag atagtttttg aaatgcctct tactaaaatt tctccattta
     6781 cagttacggt ggcaggattg atgagttctg gaatttctgg aaaatcttga gggtcttgtc
     6841 ctgaaagttt gaatttggcg ttatcggcta taatttctac agcaaaggta gaaggatcgg
     6901 taagaaaagt aagaggtgtg tcgtgataag ttttcaaagt gtcgagaagg atcttcgaag
     6961 gaatagcaat tgctcctcca tcttccatca taatgggagt aaccgtaatg ctcatggttg
     7021 tctcctgatc gctggccgta atttccagat cattttcttt ggttttgaaa agaaaatatt
     7081 ccaaaatagg cagagtgtta ctcgtagtta cgactccact aatggtttgt aagcttttta
     7141 aaagatttgt actcgaaatg atgaatttca tggatataaa tgttttgtgt ttactcggaa
     7201 agaaaaatct ttgtttgcac aacgaaggac aaatttacga atttcctcca atttagtatt
     7261 ctcttttatg aaaaattatt ttggagattt tcgactgcta tgtgatcgga gatgttatca
     7321 tactttttat tacgtcttag atgaagtata gtaaggttca gaatcgatca tccattttcg
     7381 aggaagcaaa aaattaatct ggcgaattga atatctatgg tatcctaatt tgattttcag
     7441 ggtcgagcaa gggatctaat ggaacatcct tgatgggctc ttcgggtatg aatatttctt
     7501 cttttatctt ttcgttctga tcataccact cgatgcgttt cagttgccca tcgttggtat
     7561 agaattccca ttctcccatc ttttttccat tctggtaaaa tccttttaca acttccttgc
     7621 cttccggaga atatagggag tatgggccat ctaactgatg atgcaaatag ctagcatcga
     7681 gtttcaattt cccgtcagcg aaaaattgtt tccatgggcc ttctttctgt ccatggtcgt
     7741 aagtgaagtt ttcacttacg acagtgtctt ccttatagta aacaatccac tctccgtgag
     7801 gaataccctg ttgataattt tcgatggaaa tgagtttccc gtttttctgg ttaaaatatt
     7861 cccatgtttt gtctttccat tcatcgtaga agaaacctct ggccataaga atttcatttt
     7921 cgttataaaa ctcggccgcc gaagaatgtc catcgcctgt atgaataata atggctttca
     7981 actttccatt ggggtgataa tgccggaatt cacccacggg ctgatcattc tgaaagtttc
     8041 cctcataacg aatctgtcca ttttcgtacg actttctcca taccccctgt tttcggcctt
     8101 gagcatctat ctggttgatt ccttgcgata aagctattac agaagaaaaa agaaaaaaaa
     8161 ttagctggac aataagaata ggtttctcga gtagtttcat ttcgaattca tttatacgtt
     8221 ctgcgatggc attttatagt cttggtctaa atcttctgat gacatcaatg caaatttcgg
     8281 ataattttgc attttaaatg aaataagatt ttggaaaatt atccttcgaa gtatttggaa
     8341 gctgcggtaa atgagctgtc gaaactgccg ggtataggtc gtaaaactgc tttgcgtttg
     8401 tgtttgtatt tgctcgacca acctcaggct tttgcacaaa acttgggtga agcactcatt
     8461 cgattgcggg aaaatattcg ttattgcgag caatgtcaca acattagcga tgagccggtt
     8521 tgcaggattt gtt
//
LOCUS       DAOORU010000102         8461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_10358_length_8461_cov_4.368665, whole genome shotgun sequence.
ACCESSION   DAOORU010000102 DAOORU010000000
VERSION     DAOORU010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8461
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10358_length_8461_cov_4.368665"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1267)
                     /locus_tag="PLF20_07190"
     CDS             complement(14..1267)
                     /locus_tag="PLF20_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX80818.1"
                     /translation="MKTMQKKLSNVFYVILGLPATAMGFGLSVQIAVLSWILRSKYGL
                     NIEEIGIVWAAGPIAGILGQPIVGLISDNVWFWGGRRRPFIIIGGILAALAIFALPHI
                     GFISHTLGMESIIGVAITVALTLDLSINISFNPTRSIIADVTPEGPQRTKGYTWMQAI
                     SNFFGALAYLIAALFGNYVLIYVGVVIILLFSIIPLFFITEPRHLEQSIIDNLSNTAS
                     TTKTQWGELFKIYFAHAFSWLGIQTMFVYSFAFLEQRFNVNDAQYLGQIIGWMFFILN
                     AVGSLFPIFFLEPLSRKIGRVKTHYLSLLTMSLGYFLIAFYVRSPQEIYAVFILLSLG
                     WAAVVSLPFAIMTEKVAKNKTGFFMGIFNLSVVLPQLVSSFVLGHYIEKATDKSLIYQ
                     IAAISLLVSALLWVFVRENPKKSEK"
     gene            complement(1286..3163)
                     /locus_tag="PLF20_07195"
     CDS             complement(1286..3163)
                     /locus_tag="PLF20_07195"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HOX80819.1"
                     /translation="MKIRMSLSRRKTSLIICLGFSYLLSVAQQPSIERIEPPFWWIGM
                     NESNLELLFYGKNLGDFKAYSSVPGLEIINTISIENPNYLFVYIKMDSNLKEGVYPLL
                     LKGDKDKEISVLYRFDKREPGSASRKGFGWGDNIYLLMPDRFANGNPSNDDIDAMVEK
                     ADRNNPNGRHGGDLAGVAKYLDYLKELGMTAVWLNPIFENNQPHYSYHGYAITDLYNV
                     DPRLGSNEEFVRLVKQAHQKGLKIIMDMVFNHLGDQHWMIRDLPSSSFIHRWPEYTPS
                     NFRSTTIPDPYHSEYDYQKMINGWFDRHMPDLNQNDSLLADYLIQNSIWWIEYSGIDA
                     IRMDTYQYADKDFMARWVNRIKGEYPDFSIVGELWVEQKALHAYWMEGNYRFGNYDSP
                     LQFLTDFPLYIALRESFLDKEKGWEKGVERIYYCLAQDFVYDEPRNNMIFIDNHDLSR
                     FYSIAGENLQKFTLGLVMLYTLRGIPQFTYGTEQLMTGLESDGHGLIRGDMMGGWPND
                     PVNAFEAKGRNKQQQKAWELISSLANWRKSSNVIHSGNTRHFLPENNVYSYFRYNEND
                     TVWVILNYGDEEALVETGRFAEMLGQHRRGHEIVSNREISWGETLTLQPYQALIIELI
                     P"
     gene            complement(3564..3836)
                     /locus_tag="PLF20_07200"
     CDS             complement(3564..3836)
                     /locus_tag="PLF20_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80820.1"
                     /translation="MLSIQCIDTIRLLWWNFCDPTRGSTNTSTYSTSQYNPWYPYRTN
                     NRKTISKRYDMDAFHLFIELHPFPNLGPDITVCENQPVILDAGYNL"
     gene            complement(3983..6373)
                     /locus_tag="PLF20_07205"
     CDS             complement(3983..6373)
                     /locus_tag="PLF20_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOX80821.1"
                     /translation="MKQSYWIFNCICRLFIFFLIHDVLHAQNTERYLSSNSNGHQLFI
                     HTTEGEYRFVVKTDKILEVSFRCNECLATDTSVSVVLGDRTTPWRMMKDGEFANEDWE
                     MMIRKQPFEIVFTYREKPAVVFSGYFASNESNRGFHFVIGDEKFITGGGMRALPLNIK
                     GRLLKLENRPWYGYGFEADQLNYSIPLLIMGSGYAIFFDNTAKGYADIGKTQSKILKF
                     GTRGGRMAFYLIVGENLQEITQNFTLLTGHQPLPPLWTFGHLLSRMAYRTQAQTDSIV
                     RATIQNGYPLDAIILDLFWFGDTLFHTLGNLDWYRPNWPDPIQMMNSFREMGVKTVVI
                     TEPYIVKGSHTFEEADRLGILGKNKDGRTFILDKFYFGDGGIIDVSLPKACEWMWKKY
                     DTLKKQGVAGWWVDLAEPEYHPDGIIYHGGTSELLHNAYGHLWDKMLYDYYRKYYPHE
                     RLFNLNRSGFAGSQRFGVLPWTGDVSRSWAGLKAQIPILLNMSLCGVAYVHSDAGGFA
                     QGLKDEELYIRWIQMSTFSPVFRPHGSGIPSEPIFFNPETQKIVKDFIHLRYSLLPYN
                     YSLAYQNTTQGIPMARPLFYQLKQPENDLQSWSYFWGPSFLVSPIVEKNEQKHTLWLP
                     KGKWYDWWTGEVYRGGRKIKIQNHIQQLPLFARAGCFIPMIPPIQTTDKYSTDTLIIH
                     YFLASQPHHEDFTLFVDDGKDALSIDSSHFELIHLLADQKKHALTIQLVSENHGFVHK
                     PRKRYIKMMIHTNFPMQMYLKQEEKRDKIETLQVSPLIYQFAFEWEETCFSFEIGY"
     gene            6786..8180
                     /gene="glmM"
                     /locus_tag="PLF20_07210"
     CDS             6786..8180
                     /gene="glmM"
                     /locus_tag="PLF20_07210"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOX80822.1"
                     /translation="MALIKSISGIRGTIGGKPGQGLTPIDVTRFTSAYLSLIRHHGGE
                     KHPVIVVGRDARPSGDLINGIVCATLQSMGADVIDIGLTTTPTTEIAITALQADGGII
                     ITASHNPANWNALKLLNRDGEFISSDEGEEILRISEEESFIYAELADIGRYSQNDEML
                     SHHIRRILQLPLVDVEAICRANFKIVVDTVNSSGGIAIPSLLRALGVKEIICLNCTPD
                     GNFAHNPEPLPEHLGEISEAVKKHGAHLGIVVDPDVDRLAFVCENGEMFGEEYTLVAV
                     ADYVLQHRGGPTVSNLSSTRTLKVITQKAGYEYFASAVGEVNVVELMKKTHAVIGGEG
                     NGGIIYPELHYGRDALVGVALFLSYLAHTGLTASSLRKSYPNFFMSKNKIDLPENTDI
                     RSILEEISKKYSSFPQNKVDGLKIEFPDEWVHLRRSNTEPIIRIYAESLSPDKAEELV
                     KKFIYEIEQFIPIL"
BASE COUNT         2661 a         1745 c         1633 g         2422 t
ORIGIN      
        1 aaactcagga gattcatttt tccgattttt ttggattttc ccttacaaaa acccataaca
       61 aagccgaaac gagtaaagat atggctgcga tttgataaat caatgattta tcggtggctt
      121 tttcgatgta atgtccgaga acaaaacttg aaaccaattg aggcaaaact accgaaagat
      181 taaaaattcc cataaagaaa cctgttttgt ttttagccac tttttcggtc ataatggcaa
      241 aaggcaggct tactacggca gcccatccaa gactgagcaa tatgaacact gcgtatattt
      301 cctgaggcga acgaacatag aaagctatca agaaatagcc taacgacatg gtaagcaaag
      361 agagataatg ggtttttact ctaccaattt tccgtgataa aggttcaaga aagaaaatag
      421 ggaaaagaga acctactgca ttcaaaataa aaaacatcca cccgatgatc tgtccaagat
      481 attgcgcatc gtttacattg aagcgctgtt cgagaaaagc aaaagaatag acgaacatgg
      541 tttgaatgcc caaccatgaa aaagcatggg cgaaataaat cttgaacaat tccccccatt
      601 gggttttagt tgttgatgca gtatttgaaa gattatcaat gatggactgt tcaaggtgtc
      661 tgggttcggt gataaaaaac aagggtatta tcgagaacaa caatatgatc accacaccta
      721 cataaatcaa cacataattg ccaaacagtg cagcgataag atacgccaga gcaccgaaga
      781 aattcgaaat tgcctgcatc caagtatatc ccttagtacg ttggggaccc tcgggtgtaa
      841 catcggcaat gatagaacgc gtgggattaa aactgatatt gatacttaag tcgagggtca
      901 aagccacggt gatggccaca ccgataatgc tttccattcc caaggtatgg ctgatgaatc
      961 cgatatgggg aagggcaaaa atggccaagg ctgccaagat acctccaata atgatgaacg
     1021 ggcgtctcct tccaccccag aaccatacgt tatcactaat caatcccaca ataggttgtc
     1081 ccaaaatacc ggcaataggt cctgcggccc acactatccc gatttcctca atattgagtc
     1141 cgtatttgct tcgtaaaatc cagcttaaaa cggctatctg aaccgaaagg ccaaatccca
     1201 ttgctgtggc tggcaaacct aaaatcacat aaaaaacatt gctgagcttt ttctgcatcg
     1261 ttttcataag atgaatattt gaggtttagg ggataagttc gataattaga gcttgatagg
     1321 gctgtaaagt taaagtttct ccccagctta tttcgcggtt cgagacaatt tcatgcccac
     1381 ggcgatgttg gcccaacatt tcggcaaatc tgcccgtttc tacaagggct tcttcatctc
     1441 catagttcaa aataacccat accgtatcgt tttcgttata gcgaaaatag gaatagacat
     1501 tgttttcggg caaaaaatgc cttgtgtttc ctgaatgtat tacatttgat gatttccgcc
     1561 aattagccag agagctgatc aattcccatg ctttttgttg ctgtttattc cgtccttttg
     1621 cttcgaaggc attaacaggg tcattaggcc agccgcccat catgtctccc cgtataagac
     1681 catggccgtc cgattccaga cccgtcatca gttgttcagt gccataggta aattgtggaa
     1741 tgcctcttag ggtataaagc ataacgagtc cgagagtaaa tttctgaaga ttttcacccg
     1801 ctatagaata aaatctgctc aggtcgtgat tatcaataaa aatcatgtta ttacgaggtt
     1861 catcatatac gaaatcttga gcaagacaat agtagattct ttccacacct ttttcccacc
     1921 ctttctcttt atcgaggaat gattccctca aggcaatata caaggggaaa tctgttaaaa
     1981 actgaagggg agaatcataa ttaccgaaac gataatttcc ttccatccaa taagcatgca
     2041 aggccttttg ttctacccat aactcaccca ctatactaaa atcgggatat tctcccttga
     2101 tccgattaac ccatctggcc ataaaatcct tatcggcata ttgataagtg tccattctga
     2161 tcgcatcaat gccggaatat tcgatccacc agatactatt ttgtatcaga taatcggcaa
     2221 gaagtgaatc attttgattc agatcgggca tgtggcgatc gaaccaacca tttatcattt
     2281 tctgataatc atattctgaa tgataaggat cgggaatagt ggtactgcga aaattcgaag
     2341 gggtatattc tggccatcgg tgaatgaatg aagaggaagg aagatcacgt atcatccagt
     2401 gttggtcgcc taaatgatta aaaaccatat ccatgataat ttttaatcct ttttgatgag
     2461 cctgttttac cagtcgaacg aattcttcgt tagaacccaa gcgcggatca acattataca
     2521 ggtcagtgat ggcatatcca tgataagaat aatgtggttg attgttttcg aaaatcggat
     2581 taagccaaac agccgtcatt cccaattctt tcagataatc gagatatttg gccacaccag
     2641 ccagatcccc accatgacga ccattcggat tgtttcggtc ggctttttct accatggcat
     2701 ctatatcgtc attactggga tttccgtttg caaaacgatc aggcataagt aaataaatat
     2761 tatcgcccca tccgaaacct tttcgacttg ccgaaccagg ttcacgcttg tcgaaacgat
     2821 acaaaacgct tatctcttta tccttgtcac ctttcaacaa aagcggataa acaccctcct
     2881 tcagattgga atccattttt atataaacaa aaagataatt gggattctca atagatattg
     2941 tattaataat ctccaatcct ggcactgacg aataagcctt gaaatctccc aaatttttcc
     3001 catagaacaa taattccaga ttcgactcat tcattcctat ccaccaaaac ggaggttcaa
     3061 ttctttcgat cgagggctgt tgtgcaacac ttaataagta tgaaaacccc aaacagataa
     3121 tcaagctggt tttcctgcgt gatagtgaca tcctgatctt cattcattta catttatgag
     3181 tgatatggat ataattctct aaacaatcga gtggtctgtg gatttttttc tttttatcga
     3241 cgcaaatcta aggcaaatat ttataaaaat tttcattatc agctcaaaag aaaaagtcaa
     3301 tggagaatct tcgactttta ctcaataagt cctttactgt tattctcgaa gtattccagc
     3361 aacgtgaaaa tttagccaat gtaccataaa aatcaattga gtatgatgag cctcgatgaa
     3421 ggtttcgtgc cttgaccagt gaatacatcg atattacctc tgcccataca gccattttga
     3481 gaaattacag taatactcta tgttccactg gtgtctacgg ttatgctgct cgatgcggct
     3541 tctatattcc ataagcagta ccattagaga ttgtagccag catccagaat tactggttgg
     3601 ttttcacata ctgtaatatc tgggccaaga ttggggaagg gatgaagctc gatgaaaaga
     3661 tggaatgcat ccatgtcgta gcgtttgctt atagttttac gattattcgt acgatatgga
     3721 taccaggggt tatattgaga tgtgctatag gttgaagtat tggtagagcc tctggtggga
     3781 tcacagaagt tccaccatag cagccgtatg gtatctatgc attggatgga aagcacaacg
     3841 gtattctctc acatcgacaa gtgtagtcga tgtacagatt ttgactacca aacagaaaat
     3901 tctcctttgg gaacaagata aaaattatcc tcgcttgtct cgaagactat gcaaaacagt
     3961 atattctgta atagatgctg cttcagtatc ctatttcaaa agaaaaacat gtttcctccc
     4021 actcgaaagc aaattggtaa ataagtggtg aaacttgcaa agtctctatt ttatccctct
     4081 tctcttcttg tttcagatac atctgcattg gaaaattcgt atggatcatc attttgatat
     4141 atcgtttccg aggtttatga acaaatccat gattttcgga aactaactgg atggtcaaag
     4201 catgcttttt ttggtcagcc aaaagatgga tcagttcgaa atgagagcta tcgatggaga
     4261 gagcatcctt cccatcgtcc acaaaaaggg taaaatcttc atgatgtggt tgactagcca
     4321 aaaaataatg tatgatcaaa gtatcggtag aatatttatc ggtggtttgt atggggggga
     4381 tcatgggaat aaagcagcct gcacgggcaa aaagaggcaa ttgttgaata tgattttgaa
     4441 tctttatttt tctgccaccc cgataaacct cacctgtcca ccaatcatac cacttacctt
     4501 tgggaagcca tagggtatgt ttttgttcat ttttttctac aataggtgaa accaaaaatg
     4561 aagggcccca gaaataactc cagctttgca aatcattttc gggttgtttt agttgataaa
     4621 ataatgggcg tgccatagga attccttggg ttgtattttg ataagccagg gaatagttgt
     4681 aaggtaaaag agaatagcgt aaatgaatga aatcttttac gattttttgt gtttcggggt
     4741 taaaaaatat gggttcggaa ggtattcccg atccatgagg tctaaaaaca ggtgaaaatg
     4801 tactcatctg tatccatcgg atatagagtt cttcatcttt caggccttgg gcaaatcctc
     4861 ccgcatcgga atgaacgtaa gccacaccgc aaaggctcat attcaacaaa atgggtattt
     4921 gtgccttcaa tccggcccaa ctacggctca catctcccgt ccagggtaat acaccaaaac
     4981 gttgtgaacc cgcaaaaccc gaacggttca aattgaataa acgctcatgc ggataatatt
     5041 ttcgataata atcgtacaac attttatccc aaagatgacc ataggcatta tgcagtaatt
     5101 cggaggttcc tccatgatag atgattccat cggggtgata ttcgggttcg gccagatcga
     5161 cccaccagcc agctacaccc tgctttttga gggtatcata tttcttccac atccattcgc
     5221 aggctttagg taagctcaca tcaatgatgc caccatctcc aaaatagaat ttgtccaaaa
     5281 taaaagttct tccatcttta tttttcccaa gaattcccaa acgatcggct tcttcgaaag
     5341 tatgcgagcc ttttacaatg taaggttcgg taattaccac cgttttcacg cccatttcac
     5401 gaaacgaatt catcatttga atgggatcgg gccaatttgg gcggtaccaa tcgaggttgc
     5461 caagagtgtg aaataaggta tcaccaaacc aaaacaaatc gagaatgatg gcatcgagag
     5521 gatatccatt ttggatggta gctctgacga tactgtcggt ttgagcctga gttcggtaag
     5581 ccatacggct tagtagatga ccaaatgtcc aaagaggagg caaaggttgg tgtccggtaa
     5641 gcaaggtaaa attttgcgta atttcctgta aattttcacc aacaatcaga taaaaagcca
     5701 tcctccctcc tcttgtgcca aattttaaaa ttttactttg ggttttgcct atatcagcat
     5761 agccctttgc cgtattatcg aaaaaaatag cataacccga tcccataatc aataatggaa
     5821 tgctgtaatt gagctgatcg gcctcaaaac cgtaaccata ccatggcctg ttttccagtt
     5881 tcaataatcg tcccttgata ttcaaaggaa gtgctctcat acctcctcca gtaataaatt
     5941 tttcatctcc tataacgaaa tggaaacccc gattcgattc gttcgaagca aaataacccg
     6001 aaaaaacaac cgccggtttc tctcgataag taaatactat ttcgaagggt tgttttcgaa
     6061 tcatcatttc ccaatcctca ttggcaaatt ctccgtcttt catcattctc caaggagtag
     6121 ttcgatcgcc aagaactaca cttaccgaag tatctgttgc aagacattca ttacaccgaa
     6181 aacttacttc caaaattttg tcggttttca caacaaaacg atattcgcct tccgtagtat
     6241 gtataaaaag ctgatgccca ttgctattcg atgataaata tctttctgta ttctgagcat
     6301 gaagcacatc atgtataaga aagaatataa acaaccgaca gatgcagtta aatatccaat
     6361 aagattgttt catgtgatgt tatagctatg acttttcttt aaaaaccgag ctaaagtaaa
     6421 gatttctctt gtaaattcga agatggactg ttgcttatta actattaatc tacagatttt
     6481 taaagataat gttaatggca ttccctttcg ttgatttacg ctgattgaat ttgctattcc
     6541 acgcagattt tctgtgaaca tcagctatgg aaatgattta accccctatt gtcaggataa
     6601 tatatgagac aaaagcaaat tccaaagaag aattaaacaa aagagaatag ctcgtaatca
     6661 ataaaatact aataagagaa ggaacaaaat tgatgattcc attccctatc aatatgttat
     6721 aaaaatcaaa gggaaagggt gtaaagtgag taaaaaacaa aatgtatctt tgcccaaaaa
     6781 tataaatggc attaatcaag agtatatcgg ggatacgagg tacaattgga gggaaacccg
     6841 gccaaggact cacacccatt gatgtaacca gatttacttc ggcttacttg tcattgattc
     6901 gacaccatgg gggggaaaag catcctgtta tcgtagttgg tcgagatgct cgtccatcag
     6961 gcgatctgat caatgggata gtttgtgcaa ccttacaatc catgggagcc gatgtaatag
     7021 atataggact tactaccaca cctaccaccg aaatagccat cacggcatta caagccgatg
     7081 ggggcatcat cattactgcc agccataatc ctgcgaactg gaatgctttg aagttattga
     7141 accgagatgg agaattcata tcatctgatg aaggagaaga aatattaagg atttcggaag
     7201 aagaatcttt catatatgcc gaattggccg atattggccg atattcacaa aatgatgaaa
     7261 tgctgtcgca tcacattcgc cgaattctgc aacttccctt agtggatgta gaagccattt
     7321 gcagggcaaa tttcaagatt gttgtagata cagtcaattc atcaggggga attgccattc
     7381 cgtcattgtt aagagctctt ggggtaaagg aaatcatctg tttaaactgt actcccgacg
     7441 gtaattttgc acataatccc gaacccttgc cagagcatct gggtgaaatt tccgaggcag
     7501 taaaaaaaca tggtgcgcat ctgggcatag tagtcgatcc cgacgtcgat cgtctggctt
     7561 ttgtttgtga aaatggagaa atgtttggcg aagaatatac attggtggct gtagccgatt
     7621 atgtacttca gcatcgtgga ggtcctacgg tttcgaatct ttcctctacc agaaccttga
     7681 aggtgattac tcaaaaggca ggttacgaat attttgcttc ggctgttggc gaagtaaatg
     7741 tggtggaatt gatgaaaaaa actcatgcag tcataggagg cgaaggtaac ggcggaatca
     7801 tctatcccga gctgcattac ggtcgtgatg ctctggttgg cgttgcccta ttcttgagct
     7861 atctggcaca cacagggctt accgcttcga gcttgcgcaa atcttatcct aacttcttta
     7921 tgtcgaaaaa taagatcgat ttgcccgaaa acaccgatat tcgatccatt ctcgaagaaa
     7981 tatccaaaaa atattcttct tttccacaaa ataaagtgga tggactcaaa atagaatttc
     8041 ccgacgaatg ggtgcatttg cgacgctcca ataccgaacc cattattaga atatatgccg
     8101 agagcttatc gccagacaaa gccgaagaac tcgtaaaaaa atttatatac gaaatcgaac
     8161 aattcatacc gattttatag ataaacgcgt tcgaactata cttgacttat gccataccta
     8221 tagatttatc gaaaacaaat accatccctc ggattacctg actaacttat tgaaaacgtt
     8281 tgcatagcca tgcccaaaaa gaaaaaacga aaaccactaa aatcgaaaaa actaatcatc
     8341 aagttaggtg cttctcaaat ggaacttctc aataattttt gcattcggca tcaaaccaca
     8401 cccaacaaag tgatcaaaca aagcctaatg acctacctca ataattgtaa acctgaggat
     8461 t
//
LOCUS       DAOORU010000103         8446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_10381_length_8446_cov_4.585627, whole genome shotgun sequence.
ACCESSION   DAOORU010000103 DAOORU010000000
VERSION     DAOORU010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8446
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10381_length_8446_cov_4.585627"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..2171
                     /locus_tag="PLF20_07215"
     CDS             156..2171
                     /locus_tag="PLF20_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOX80823.1"
                     /translation="MEYKKLKIVKEDPWLEPVEKEVFDRYQRYINRLNEITSQWGSLL
                     KFANAHNYLGIQYSKKDKGWYYREWAPRAFDLYLMGDFNNWDRFSHHLEKKDNGIWEI
                     FLPYDLYKDKFIHGSKVKVLVHGRNGWHERIPVYIRRVVQDPNTKDFTGQLWFPAHPY
                     DWEGDNYRISGKEAPYIYECHIGMAQEEPRIGTYREFADNILPHIKTCGYNMIQIMAI
                     AEHPYYGSFGYHVSNFFAPSSRFGTPEDLKYLIHKAHLMGLGVIMDIVHSHTVKNYNE
                     GLNDFDGDEGLYTHIGPRGDHPAWDSKLFDYGKTEVLQFLLSNIAYWMKEFHFDGFRF
                     DGVTSMMYFHHGFVGNWTRELYFLDGVEWDAITYLQLANTLVHKIRPDSLTIAEDVSG
                     MPGLCSPISHGGIGFDYRLGMGIPDYWIKILNERKDEDWDMDELYHAMLNRKFGVKTV
                     AYCESHDQALVGDKTIAFRLMDQEMYWHMRIDDANLVIDRGIALHKLIRIFSLSLGGE
                     AWLNFMGNEFGHPEWIDFPREGNCWSYWYARRQWSLAYDPNLKYKFLLAFDKAMIHLA
                     KTSDIMRSPYPNLLNIDQHHKTIVFERAGLIFIFNWHPTLSIPDYAFPVPEPGVYRII
                     LCSDDGQFGGHQRVNTHLDYLAIPGPEGTGVARIYNINRALIVLQKV"
     gene            2171..2740
                     /locus_tag="PLF20_07220"
     CDS             2171..2740
                     /locus_tag="PLF20_07220"
                     /inference="COORDINATES: protein motif:HMM:TIGR02985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HOX80824.1"
                     /translation="MKNSHPLSNSGLSEENFKAIFLEEFSNLKAYAQSIVKENETSSD
                     IVQEAFMALWENRKIFENKRDPKPYLATIIRNKSLNYLRNNKKYHPDLEAAEGWEQHL
                     GISCNDQFTQKQLKAKIQETLDHLPPKCREIFLMNRQEGLKYKDIAFRLGISIKTVEA
                     QMAKALKAFHDNLDQFILLTLIFLMNIFF"
     gene            2796..3812
                     /locus_tag="PLF20_07225"
     CDS             2796..3812
                     /locus_tag="PLF20_07225"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOX80825.1"
                     /translation="MDVPEDILELITSYFSGELNEEGIQQLIDWLNKSSENRKLFLDY
                     KKAWELQASILKQPVNPEEEWEKFRKKNLSTFTKKEIKEERVKKLMINRSYWRIAALL
                     ALIIISTFAAIYLFRNEEVEILLTQKTKETFTLPDGSTITLAPESKAIYDKTYGKKER
                     KIIVTGWVYLEVAKNERLPFRVWAGNLEILVTGTSFYIHSSATEQDASVILESGKVKA
                     YPKGKPSEAIELKPGQAASYVSSKDIITITSPEPNYAAWNTHKFQFNNTSLDEIIALI
                     ESVYSIHIDLSSPELGSCKLTATFENQSPESILQVIASALHLKLGGDDTSFILSGMPC
                     QSNK"
     gene            3812..6424
                     /locus_tag="PLF20_07230"
     CDS             3812..6424
                     /locus_tag="PLF20_07230"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOX80826.1"
                     /translation="MAKRCSIFVFFFLIWNYQAYLQENISTYTYRARQIPLQQVIHDL
                     EIITGLPFAYSPERIPLTKKISCKVKTNHLTTFLDEVFERNGIQWENINGQIVLTGTI
                     EPLKKQKIVISGIISDSTSGALIPGVLLTTESGKELAFSNSYGYFSVEIPIETKSLFV
                     SCVGYKTQTVKLNYPNIDLLWIKLNPLVIEMKAIEIKATYKNEDKASFGVNIDQSIWG
                     MGGQNDIIQVLNSVPGFHLFGDGSTRFFVRGGESNQNLILIDESPIYNPSHLFGFFNA
                     LAPEAISQVNAWKGDAPARYGGGLASIIDVHVREGNMYKNIISGNLGPYASSLTLEGP
                     IIKEKLSYLISARRSNMEWLKPSNYTPNSFNFYFYDINLKINSILNTRNRLYLSFYRG
                     EDNISQLTESSIKTFGMRWNNLTGNFRWNYIPTNKIFLNTTLAFNKYSYFLFLDARHN
                     SYWKSSIEGLHLKSDLTWYVNADNTLRTGIFIENLIADQGNIYPSRIDSLNYTKSFPF
                     YRSLSSQLYIEHQAALLDKRLVINYGFRMPIWSDYGPFYVHYYNPEYEPFYTDTIGKD
                     RIYYTYIGFEPRIHLSFDVQPHWKIKLNYNRTLQFFQSLSNSISPFSTIENWAPAGPN
                     ISPQIGDLFSIGSENYFLQNTYSLLLEAYLRFAHNQIDYKEHANLLYNAYQEGELRFG
                     KAKAYGIEMWLKKNTGKLSGWLGYTYSKVSRETKDINDNQPYYPYYDRPHQIQIRLNY
                     HPSQRISLYASWYYMTGGKTTIPSGFYDNNNFIIPIYEEKNNARLPDYHRMDIAAEFQ
                     LNKKDARFRQTLSVSIYNIYNRNNPFLVSFNKIMDDKGNFVVPANFYQKQIIIPTQLS
                     VAGIIPTIIYKFSL"
     gene            6426..7244
                     /locus_tag="PLF20_07235"
     CDS             6426..7244
                     /locus_tag="PLF20_07235"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 family protein"
                     /protein_id="HOX80827.1"
                     /translation="MKKCIVYFIVVIFLYGCEKEFAPDLSSEVNKKVVINALFTDEAN
                     ENEVEIRWSVRQPNDVPSPINEAQVILSSIDEVIIFKLDSLKPGIYRAKMNLKSNQEY
                     TLTVSVEGQVFSARAELAEGKAFNPPTFSFSNDEKLYYLDKVAIPFDPSSPAIYQITA
                     DWSEVSGYTQLPSEQTHAVIYFFSLSTLDVSQLLPPASEKIGFPAGTIMDVKRFALTQ
                     SHAEYWRQVLLETSWAGGLFATEPANIISNFSNDALGWFGLCGTTQLSVIVGEK"
     gene            7315..8244
                     /locus_tag="PLF20_07240"
     CDS             7315..8244
                     /locus_tag="PLF20_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80828.1"
                     /translation="MEKHLLSRYLLPFVIGVCLAGCDKNNETEPEVDTSDAQQLIQED
                     NLFRANVNEITLEAALLSLESNSKLGNIDYLCNVNIDSITLAGDSTLIYVTFKGENCS
                     GTRSRYGKMIIHRKTSTYWIQAGTSIIIEVKDYSITNLTSNKTMVLNGKIITQNVSGG
                     NIVLLGLQQQSIIHRSQGYMHTLFPDGSTRLWHHARQTIFTHSFNNLIITEDGMGNID
                     GYEKLICWGTLRNGQKFYNQISEPVTRKRECNFLPYAGIQKHIIPQNNMTVTTTFGFN
                     SNNKPINVGKCPSRLRFQWQKQNKSGILFLPLE"
BASE COUNT         2785 a         1566 c         1569 g         2526 t
ORIGIN      
        1 aaatattacc atacccgatg aacattggaa aataatcgta aatggcgaca gatttttatc
       61 ctctcctgta ccctataccg aaaaaggacc tcttactata cccgcatctt cagtcattat
      121 acttgcagtt actgaataat actctttatt tctctatgga atacaaaaaa ttaaagatag
      181 tcaaagaaga cccttggttg gagccagtcg aaaaagaggt tttcgatcgt tatcaacgtt
      241 atataaatcg tttgaacgaa ataactagcc agtggggcag ccttctgaaa tttgccaatg
      301 cacacaatta tctgggcatt caatacagca aaaaggacaa ggggtggtat taccgagaat
      361 gggctccacg tgcattcgat ctttatttaa tgggcgactt caataattgg gacaggtttt
      421 cgcatcattt ggagaaaaaa gacaatggga tatgggaaat ttttctccct tacgatttat
      481 ataaagataa attcattcat ggcagtaagg tgaaagttct ggttcatgga agaaatggct
      541 ggcatgagcg tattccggtt tatatcaggc gagtcgttca agatcccaat accaaagatt
      601 ttacaggaca gttatggttc cccgcccatc cttatgattg ggaaggtgat aattacagaa
      661 tttcgggcaa ggaagctcca tatatttacg aatgtcatat cggtatggct caggaagaac
      721 ctcgaatagg aacctatcgt gaatttgccg ataatatact tccgcacatc aagacctgtg
      781 gatataacat gatacaaatc atggccatag ccgaacatcc ttattatggt tcgtttggtt
      841 accatgtgag caatttcttt gcaccctcct cacgttttgg tacaccagaa gacctgaaat
      901 atttgattca taaggctcat ttaatgggtt taggagttat tatggatata gtgcactcac
      961 atacagtgaa aaattataat gaaggtttga acgatttcga cggtgatgaa ggtttatata
     1021 ctcatattgg ccccagaggc gatcacccag catgggactc caaactgttc gattatggaa
     1081 agacagaagt attgcaattc ttgctttcca atattgccta ctggatgaaa gagttccact
     1141 tcgatggttt tcgttttgat ggagtaacct ccatgatgta ttttcatcat gggtttgtgg
     1201 gtaactggac tcgtgaactt tattttttgg atggagtgga atgggatgcc attacctacc
     1261 ttcaattggc taacactctc gtacacaaaa tcagacccga tagtctcacc atagctgaag
     1321 atgtgagcgg aatgccagga ctttgcagcc ctatctctca tggcggaata ggatttgact
     1381 accgtttggg aatgggaatt cccgattatt ggattaaaat attaaacgaa agaaaagacg
     1441 aagactggga catggatgaa ctttatcatg ccatgcttaa tcgcaagttt ggggtaaaaa
     1501 ccgtagccta ttgtgaatcg cacgatcagg cactggtggg tgataaaacc attgcattca
     1561 ggttgatgga tcaggaaatg tattggcaca tgagaataga tgacgcaaat ctcgttattg
     1621 atcgaggaat tgctctgcat aagttgatac gtattttttc actttctctg ggtggtgagg
     1681 cttggttaaa cttcatgggc aatgagtttg ggcatcccga atggatagac ttccccagag
     1741 aaggtaactg ctggagttat tggtatgcgc gtcggcaatg gtcactggct tatgatccca
     1801 acctaaaata caagtttttg ctggctttcg acaaagccat gatacattta gcaaagacct
     1861 ctgatattat gcgctcgcct tatcccaatt tgttgaacat cgaccagcat cataaaacca
     1921 ttgttttcga acgcgctgga ctaatcttta tctttaattg gcatcccacc ttaagcattc
     1981 ccgattatgc ctttccagtt cccgaaccgg gtgtttatcg catcatactt tgtagcgatg
     2041 acggacaatt tggtggacat caaagagtaa atacacatct cgattatttg gccattcccg
     2101 gccccgaagg tactggtgta gcaagaattt acaatatcaa ccgcgctctt attgtacttc
     2161 aaaaagtttg atgaaaaaca gtcatccatt atcgaattct ggactttcgg aagagaattt
     2221 caaggctatt ttcttggagg aattttctaa tcttaaagcc tatgcacaat ccatagtaaa
     2281 agagaatgaa acctcctcag acattgttca ggaggctttc atggcactat gggaaaaccg
     2341 caagattttt gaaaataaac gcgatcccaa accttattta gctactatta ttcgaaacaa
     2401 atcacttaac tatttacgta ataataaaaa atatcatccc gatctcgaag ctgctgaagg
     2461 ctgggagcaa catctgggca tatcatgcaa tgatcaattc actcaaaaac aattaaaagc
     2521 gaaaatacaa gaaacccttg accacctccc tccaaaatgt cgtgaaatct ttctgatgaa
     2581 cagacaggaa ggattaaaat ataaagatat tgccttcaga ttgggaattt ccattaaaac
     2641 tgtcgaagct caaatggcca aggctttaaa agctttccat gataatctcg accagtttat
     2701 cctattaaca ttaatattct taatgaatat tttcttttaa tttaacataa gggtaaatga
     2761 atgatagagt gttttatata aaggaggaat agaccatgga cgttccagaa gatattcttg
     2821 aattgattac tagctacttt tcaggcgaac tgaatgaaga aggaattcag cagctcattg
     2881 actggttaaa taaatcaagc gaaaacagga aactattttt agattacaaa aaagcatggg
     2941 aactgcaggc aagcatcttg aaacaaccag tgaatccaga agaagaatgg gaaaagttca
     3001 gaaaaaagaa tttatctacc tttacgaaaa aagaaataaa ggaagaaaga gtaaaaaaat
     3061 tgatgataaa tcggtcgtat tggcgtatag cagctttact ggccttgatc attatttcga
     3121 cttttgcggc catttatctt tttaggaatg aggaagtaga aattttactc acccaaaaaa
     3181 caaaagagac ctttactttg cctgatggat caacaattac gctggcacct gaaagtaagg
     3241 caatttatga taagacgtat ggcaaaaaag aaagaaaaat tatcgttaca ggttgggttt
     3301 atttagaagt tgcaaaaaac gaaagacttc catttagggt atgggctgga aatttagaaa
     3361 ttttagttac aggaacatca ttctatattc attcttctgc tactgaacaa gatgcctctg
     3421 ttatactcga gagcggaaaa gtgaaagcct atcccaaagg aaagccttcc gaagccattg
     3481 aacttaagcc aggtcaggca gcttcttatg tctcaagcaa agacattatt actattacct
     3541 ctcctgaacc gaattatgca gcatggaata ctcataaatt tcaatttaat aatacatctc
     3601 ttgatgaaat cattgctcta attgaaagcg tttatagtat tcatatagac ctttcatctc
     3661 ctgaattggg cagttgcaaa ctaaccgcaa catttgaaaa ccaaagccct gaatccatac
     3721 ttcaggtgat tgcatctgca cttcatctga aacttggagg tgatgataca tcattcatcc
     3781 taagcggtat gccctgtcaa tcaaataaat aatggcaaaa aggtgcagta tatttgtttt
     3841 cttcttcctt atttggaatt atcaagccta tttacaagaa aatatatcta catatacata
     3901 ccgagctcga caaattccac ttcaacaagt cattcatgat ttggaaatca taacgggttt
     3961 accttttgca tacagccccg agcgcattcc tttaaccaaa aaaatttctt gcaaggtcaa
     4021 aacaaaccat ttgacaacat ttcttgatga ggtttttgaa agaaatggga tacagtggga
     4081 aaacatcaat ggacaaatcg tactcacggg gacaattgaa ccattaaaaa aacaaaaaat
     4141 agttataagt ggaataattt ctgattctac cagtggagcc cttattcctg gagttttgct
     4201 tacaacggaa tcgggcaaag aacttgcctt tagtaattcg tatggatatt tttccgttga
     4261 aatacccata gaaacaaaaa gtttgtttgt aagttgtgtt ggatacaaaa ctcaaacggt
     4321 gaagctgaat tatcccaata tagatttatt atggataaaa ctcaatcctt tagtgataga
     4381 aatgaaggcc atagagatta aagctacata taaaaacgag gacaaagcga gttttggggt
     4441 caatatcgat caatctattt ggggaatggg agggcaaaat gatatcatac aagttttgaa
     4501 tagcgttccc ggttttcatt tatttggaga tggcagtaca agattttttg tgcgaggtgg
     4561 ggaaagtaat cagaatctaa tccttattga tgaatctcct atttataatc cttctcatct
     4621 ttttggattt ttcaatgctt tagctccaga ggctatcagc caagtaaatg cttggaaggg
     4681 agatgctcca gcacgatatg gaggaggatt ggcatctatc attgacgtcc acgttcgtga
     4741 aggaaacatg tataaaaata taattagcgg aaatttaggg ccttatgctt cctcactcac
     4801 tcttgaagga cccatcatca aagaaaaatt aagttatctt atttcagcac gcagatcgaa
     4861 tatggaatgg ctaaaaccct caaactatac tccaaattcc ttcaatttct atttttatga
     4921 tatcaatctg aaaattaata gtatactgaa tacccgaaac agattgtacc tcagtttcta
     4981 tagaggtgag gacaatattt cacaacttac cgaatcatcc ataaaaactt ttggtatgag
     5041 atggaacaat ttaactggca acttccgctg gaattatatc ccaactaata agatattcct
     5101 taatactacc ttggctttca acaagtacag ctatttttta tttcttgatg cccgacataa
     5161 ttcttactgg aagtcgtcaa ttgagggact gcatttaaaa tctgacctta catggtatgt
     5221 aaatgctgac aataccttac gcacagggat tttcatcgaa aacctcattg cagatcaagg
     5281 aaatatttat ccatcgagaa ttgacagctt aaactataca aaatcttttc ctttttaccg
     5341 atctctttct tcccagctat atatcgagca tcaagctgcc ttacttgaca aaaggctggt
     5401 aattaactat ggatttcgta tgcccatttg gagcgactat ggtccctttt atgttcatta
     5461 ttataatccc gagtatgaac cattttatac ggatactatt gggaaagata gaatctatta
     5521 tacttacatt ggttttgaac cccgcatcca tttaagtttt gatgttcaac ctcattggaa
     5581 aattaaatta aattataaca gaacattgca gttttttcaa tcactttcca attctatcag
     5641 tcctttttcc accattgaaa actgggcacc tgcaggtccc aatatttctc cacaaatagg
     5701 agatttattt tctattggta gcgagaacta ttttcttcaa aatacctatt cattattatt
     5761 ggaagcatac ttgagatttg cccataacca gattgattat aaagaacatg ctaatttact
     5821 gtataatgca tatcaagaag gtgaattacg tttcggaaag gcaaaagcat atggaataga
     5881 gatgtggttg aagaagaata cgggtaaact ttcaggatgg ttaggttata cctacagtaa
     5941 ggtaagccgt gaaacaaaag atattaatga taatcagcct tactaccctt attacgatcg
     6001 gccacatcaa attcagattc gacttaatta ccatccatcc cagcgcatca gtctttatgc
     6061 ttcttggtac tacatgactg gaggtaaaac cactattccg tctggatttt atgataataa
     6121 taactttatt attcctattt atgaagaaaa aaataacgca cgtttacctg attatcacag
     6181 aatggatatt gcagccgaat ttcaattaaa taaaaaagac gcccgttttc gacaaactct
     6241 aagtgtgagc atctacaaca tttataaccg aaacaatcct tttttagtca gttttaataa
     6301 aattatggat gataaaggga attttgtggt tccagcaaat ttctaccaaa aacaaatcat
     6361 tatccccact caattatcgg tagccggaat tattcctact atcatttata aattcagtct
     6421 ttgaaatgaa aaaatgtata gtctatttta ttgttgtaat ttttttatat ggttgtgaaa
     6481 aagaatttgc acctgattta tcttctgaag taaacaagaa agtagtgatt aatgcgctat
     6541 ttaccgatga agcgaatgag aatgaggttg aaataagatg gtcagttcgt cagccgaatg
     6601 atgtcccatc tcctattaat gaagctcagg taatattaag ttcaattgat gaagttatca
     6661 tatttaagtt agacagttta aagccgggaa tatacagagc aaaaatgaat ttaaaatcca
     6721 accaggaata tactcttacg gtgagtgtgg aaggacaggt tttttcggcg agagccgagc
     6781 ttgctgaggg caaagccttt aaccccccca ctttttcatt ttctaatgat gagaaattat
     6841 attatttgga taaagtagcc attcctttcg atccatccag tcccgccata tatcaaataa
     6901 cagccgactg gtcagaggta tcgggatata ctcagcttcc ttctgaacaa actcatgctg
     6961 taatatattt ttttagtttg tcaaccttag acgttagcca acttttgcct ccagcttctg
     7021 aaaagattgg gtttccggca ggtacaatca tggatgtaaa acgttttgca ttgactcaga
     7081 gtcatgcaga atattggcga caggtattgc tcgaaacaag ctgggctgga ggattatttg
     7141 ccacggaacc tgcaaatatc atttcaaatt ttagcaatga tgccttaggt tggttcggat
     7201 tatgcggcac tacacagctt tcggtcattg ttggagaaaa ataatcgatc ctacataagg
     7261 gtaaatctta gttcatgtgt attttttata gaaaacatta taaaaattct taccatggaa
     7321 aaacatctat tatcacgata ccttctgcca tttgtgattg gcgtatgcct tgccggatgc
     7381 gacaaaaata acgaaacaga accagaagta gatacatccg atgcgcagca gcttatccaa
     7441 gaagacaacc tttttagggc caacgtcaat gaaattacgc ttgaagctgc cctcctctct
     7501 cttgaatcaa attcaaaatt ggggaatatt gattatttat gcaatgtaaa tatcgactcc
     7561 ataactctgg caggtgatag tacccttatt tatgtgacat ttaagggaga aaattgcagc
     7621 ggtacacgct cacgctacgg aaagatgatt attcaccgca aaacttctac ctattggatt
     7681 caggcaggaa caagcatcat catagaagta aaggactatt ccattaccaa ccttacctcc
     7741 aataaaacca tggtattaaa cggaaaaatt attacacaga atgtcagcgg cggcaacatc
     7801 gttttactcg gtttgcagca acaatcaata atccatcgaa gtcaaggata catgcacacc
     7861 cttttccccg atggaagcac aagattatgg catcatgcgc gccagactat atttacgcac
     7921 tcttttaata acctcatcat tacagaagat ggaatgggga atatagatgg ttatgaaaag
     7981 ttaatctgct ggggaacttt acgtaatggg caaaaatttt ataatcagat ttcggaaccc
     8041 gttactcgaa agcgagaatg caatttccta ccttatgctg gaattcaaaa acatattata
     8101 ccccaaaata atatgacggt tactacaact tttggtttta atagcaataa taagccaata
     8161 aatgttggga aatgcccatc aagactacgc tttcaatggc aaaaacaaaa taaatctgga
     8221 atattgttcc tacccctcga ataattttaa atattaaaaa ataaatcata tataacccta
     8281 aaatttaaat gaaatgaaaa cacaaaaatt gattttcgga atttttacga cagttgtatt
     8341 tgcgagtttg ctctctttaa cgagttgcaa caaggatgaa gataaatctg caaataatga
     8401 taatgaaacg agtgccacat tcgttactct aagtcaggac gaaaac
//
LOCUS       DAOORU010000104         8419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_10425_length_8419_cov_6.192372, whole genome shotgun sequence.
ACCESSION   DAOORU010000104 DAOORU010000000
VERSION     DAOORU010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8419
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10425_length_8419_cov_6.192372"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1399)
                     /locus_tag="PLF20_07245"
     CDS             complement(47..1399)
                     /locus_tag="PLF20_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOX80829.1"
                     /translation="MAKYSIIDVETTGTSVKGERITEIAIFNHDGQKITDEFVTLVNP
                     EKKIPYRISQITGITDRMVENAPRFCEIARKVLELTEDSVFVAHNVGFDYGFVKEEFK
                     NLGYRFERDKLCTVRLSRKLLPHHPSYSLGNICRDLGIQIFDRHRAGGDALATVKLFE
                     YLLSIDPNLLKINLKGINSSISAATLHSLPEEAGVYYFYDSSQQIIYIGKSKNIHNRV
                     VSHLNSETTRKALEMKHAIADISYEITGSDLIAQLLESEEIKKHKPLYNRQQRRTGFR
                     IGLFTYFDEKGYMRLKIDKLSDGLLPVTTYNSVMSAKRALFALCDEYHLCQKLCGLYK
                     TKGACFQHGIGQCMGACIGLESTADYNARVEAAITPVKFEHENFFIIDRGRCPEEYSV
                     VEVRHGAYLGFGYFDGNDFDGTADFLHSCIHRFSDTRDARRIILSCLKHHEFERLLVY
                     "
     gene            complement(1454..2254)
                     /locus_tag="PLF20_07250"
     CDS             complement(1454..2254)
                     /locus_tag="PLF20_07250"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HOX80830.1"
                     /translation="MYFASNIKLLRKRKGRTQDDVALALNMKRPTLSGYENAVAEPPL
                     SVLAQFSEYYKLSIDTLLKVDLSKLPESQLSQIERGYDPFVRGSQLRVLATTVDAQNE
                     ENIELVNEKAKAGYATGFADPDYVSILPTFRLPFLSRNKKYRTFQISGDSMLPIPDGA
                     YVTGEFVQNWLSIHDRQPYIILTLDDGIVFKVVENRIAQEGKLRLYSLNPVYQPYDVD
                     IRDVREVWKFVNYITNQFPEPLPSSDLIYQTLNEVKDDIKSIRKKLDE"
     gene            2358..3524
                     /gene="dinB"
                     /locus_tag="PLF20_07255"
     CDS             2358..3524
                     /gene="dinB"
                     /locus_tag="PLF20_07255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOX80831.1"
                     /translation="MSTKYINEPGRTILHLDMDTFFVSAERLYNHKLEDKPVIIGGMG
                     DRGVVASCSYEARRFGVHSAMPVRMAKILCPEAIFIRGDMELYTRLSRMVTQIIAEKA
                     PLFEKASIDEHYLDLTGMERYFGCYKWAHELRQSIIHHTGLPISLGLSVNKTVAKMAT
                     GEAKPNGEKEVPSMEVKNFLNPLPIHKIPGIGEKTYQTLRSMGIVNIQTLSAIPPDIM
                     ETLLGKNGIILWKRANGIDTTPVIPYSEEKSIGHETTFDQDTIDVNKITNTLLRMTER
                     LTFELRKKQKLTSCVTVKIRYANFDTHSLQRQIPYTSLDHELFPVVKNLFEKLYQRRM
                     LIRLVGVRFSHLIHGFHQLDVFDPSPRLIDLYKAMDHIRLRYGPKAIRRGSTFE"
     gene            3675..4844
                     /locus_tag="PLF20_07260"
     CDS             3675..4844
                     /locus_tag="PLF20_07260"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HOX80832.1"
                     /translation="MKLLYLCRLCYRDWKHEGKMEWHPHRKTSLILLIFLQNATSISA
                     QGKNENNFLTFSASYVGEGFANFSGGIKTGLTYLGLVSLNTYFDASKAHLWEGGGFFL
                     SLYNTHGGTPATDFVGDYQGVSNIEAGNHTYLYELWYAQKFRKVKGIIGMQDLNVNYA
                     ASQYGNSFINSSFGIPSTFAYNISSPIFPLTALGFHLEWQPTEHFRWKAAIFDGTPEG
                     LENFHLKWKLNKQDGFLSITEIEWTKNWKGNHSGSYKVGAYFHNSQDSLSQSQENGGF
                     YFVGDQTINEHIGIFSQIAVSPSSKNSNPVYAGLGLNYKGIISKRQKDILGLAVAYAG
                     LKDRADETALETMYHFIINEHIPLIPDFQYIINPAGQDIALKNSLVGFLRMRFDF"
     gene            4858..7347
                     /gene="feoB"
                     /locus_tag="PLF20_07265"
     CDS             4858..7347
                     /gene="feoB"
                     /locus_tag="PLF20_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOX80833.1"
                     /translation="MEETNNFKRLSDLSPGDEAIIVKVLGHGSFRRRLAEMGFVRGKK
                     VKVIKNAPLQDPVEYEIMGYNISLRRSEAELIQVISVQDAAAFTRNKHFNGTIDEETL
                     KVSALEKEKSIHVALVGNPNSGKTTLFNRISGSHERVGNYGGVTVDVKEAEVKKNAYA
                     IKLVDLPGTYSITEFSPEELYVRVYLTEKMPDIVVNVVDASNLERNLFLTTQLIDMDL
                     KVVIALNMYDELQKKGDRLDYKYLGKMLGIPIVPTIATKGEGIEELLQKLIDVYEDKD
                     PIVRHIHINYGSIVEKAIKQIQDVIWQNPAITDKLSSRYLAIKLLEGDKTILSELEKY
                     DNYPQIESTTRQAIQSLEKEYGDSCETVITQLKYGFIEGALKETYHLSKQKHKEKQQT
                     LDNLFLHKFWGYPIFLFFVWLMFQSTFSLGKYPTQWLESGINLLGSWLGRIMVPGPLC
                     DLLVNGIIGGVGNVLVFFPNILILFFFVSILEDTGYMSRAAFLMDKLMHKIGLHGQSF
                     IPLVIGFGCNVPAILATRTLKNRKDRILTIIIIPFMSCSARLPVYLLLISAIFPKNQA
                     LILFSLYSIGIFMAIITALFMKRTVFKEEESPFVMELPPYRIPTLKTINIHMWDKSRE
                     YLRKIGNIILVASIIIWAMGYFPRHPEIDKKYEEQILSVQNNTTLSKGEKEKTVNQLQ
                     TTKMAELQQKSIIGQLGKVVEPAIRPLGFDWKMGIGLFTGLAAKELVISTMGVLYQVD
                     NSGDNSVSLQEKLKNEVHTAGKLKGEKVFTPLVAFAFMVFILLYVPCVATIAAMKKEA
                     GWQWAVFGVVYNTLVAWLMAFLIYQIGNCIV"
     gene            complement(7510..7779)
                     /locus_tag="PLF20_07270"
     CDS             complement(7510..7779)
                     /locus_tag="PLF20_07270"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOX80834.1"
                     /translation="MTTHEELKNEVLTLADEVGVKPNAIHIRAMKRKWASCSSKGRLS
                     FNEILLDEPKEVRYKIILHELLHLKYPNHGKMFKLLLDYYLQKVL"
     gene            complement(7781..>8419)
                     /locus_tag="PLF20_07275"
     CDS             complement(7781..>8419)
                     /locus_tag="PLF20_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80835.1"
                     /translation="MYQILRENYDRGIDIKKDFTRKTIELVKKHTKSGEIQPTIDIFE
                     INEETLEKIEKSKASDIEKVFNLIKSIERTVAQEGDTAPYLKSIAEKAEMLAKLFQDR
                     QKTTRETLEVLKKIIEEIVTAKKEQEEKKMKSEIFSIYWILDRSGFENATSMANEMEK
                     VFAQYPHWHKSNKHGLKIRQKLYEVLVHSGITDSKKISETAQQIMKLLNVKI"
BASE COUNT         2541 a         1592 c         1694 g         2592 t
ORIGIN      
        1 ctgactttcg aaattaaaaa taaagaagtt taaatagtcg aatcggttaa taaaccaaca
       61 aacgttcgaa ttcatgatgt ttgaggcatg aaagaatgat acgtcgagcg tcgcgggtat
      121 cactaaaacg atggatacag gaatggagaa aatcggccgt tccgtcgaaa tcgtttccat
      181 caaaatagcc aaaacccaga taagcaccat gcctaacttc tactaccgaa tattcttcag
      241 ggcaacgtcc tctatcgatg ataaaaaagt tttcatgttc gaattttact ggagttatgg
      301 ccgcttcaac acgagcattg taatcggctg tagattccaa tcctatgcaa gctcccatgc
      361 attgaccaat gccatgctga aaacaggcac ctttagtttt atacaagccg caaagtttct
      421 gacaaagatg atattcgtcg cataaggcaa aaagagcacg cttagccgac atgaccgagt
      481 tgtaggtagt aaccggaagc aaaccatccg atagtttatc tatcttcagt cgcatataac
      541 ccttttcatc gaaataagta aaaagcccaa tacgaaatcc tgttcgtcgc tgttgacggt
      601 tatacaaggg tttatgtttt tttatttcct ccgattcgag taactgagca atcaggtcgc
      661 tgcctgtaat ttcataagaa atatcggcaa tggcatgttt catttcaaga gccttgcgtg
      721 tggtttcgct attcaggtgt gaaactacac ggttgtgaat gtttttgctt ttgcctatat
      781 agatgatctg ttgagatgag tcataaaagt aataaactcc ggcttcttcg ggcaaggagt
      841 gtaaagtagc cgcagaaata gaagaattta tgcctttgag gttgattttt aagagatttg
      901 ggtcgatgct caataaatat tcaaacagtt tgaccgtagc cagcgcatct ccaccagccc
      961 tatgccggtc gaagatttga atacccaaat cccgacaaat atttccaaga ctataagaag
     1021 gatgatgggg aaggagttta cggctcagtc gaaccgtaca aagtttgtcg cgctcaaaac
     1081 gataacccag atttttaaat tcttctttca caaaaccata atcgaatcct acattatgcg
     1141 ccacaaaaac ggagtcttcg gtcagctcga gcactttgcg ggcaatctca caaaaccggg
     1201 gtgcattctc taccattctg tcggtgatgc cggttatctg gcttatgcga taaggaattt
     1261 ttttctctgg attcactaaa gtgacgaatt catcggttat cttttgccca tcatgattaa
     1321 atatggcaat ctcggtgatg cgttctccct taaccgaagt acctgttgtt tcgacgtcaa
     1381 taatagaata tttcgccatt tgctaagaga taatggactt aataaattga ttgtgaataa
     1441 aactgggaga atctcattca tctaattttt tacggattga ctttatatcg tctttcactt
     1501 cgtttaaggt ttgatagatg agatcggagg aaggcagagg ttcaggaaac tggttagtaa
     1561 tgtaattgac gaatttccaa acttcgcgta cgtcgcgtat atctacatcg tagggttgat
     1621 atactgggtt gagggagtag agtcggagtt taccttcttg ggcgattcgg ttttccacca
     1681 ctttaaacac tataccgtcg tcgagggtaa gaatgatata aggttgtcgg tcgtggatgc
     1741 tgagccagtt ttgtacgaat tcgcctgtga cgtaagcgcc gtcgggaata gggagcatag
     1801 aatcgcccga aatttgaaag gtgcgatatt tcttattacg cgagagaaaa ggcagacgaa
     1861 aagttggcag tatgctcaca taatcggggt cggcaaaacc cgtagcatag cctgctttgg
     1921 ccttttcgtt gaccagttcg atgttttctt cattttgtgc atccacagta gtagccagca
     1981 cccttaattg ggagccacgc acaaaaggat cgtatccgcg ttctatctga gaaagttggc
     2041 tttcgggcag cttcgataag tcaaccttta aaagagtatc gatagaaagc ttgtaatatt
     2101 ctgagaattg tgccaataca ctaagaggtg gttcagctac tgcgttttca tatccgctga
     2161 gggtaggccg tttcatgttc aaagccaggg ctacatcgtc ttgagtgcga cccttacgct
     2221 tgcgaagcag tttgatgttg gatgcaaaat acataagaca attttttttt gcaaagataa
     2281 catttttcat aaaaaatgat tatattgcca tcgaaatttt gattaaaaaa taatcattta
     2341 ctatgaaatg catgcctttg tcaacgaaat atataaatga gccgggacgt actattttgc
     2401 atctcgacat ggacacgttt tttgtttcgg ccgagcggct ttacaaccac aagctcgaag
     2461 acaaaccggt aatcattggt gggatgggcg acagaggcgt ggtagcctca tgcagttatg
     2521 aagccagacg ttttggggtg cactcggcaa tgccagtacg tatggccaaa atactttgtc
     2581 ctgaagccat ttttattcgt ggcgatatgg aactctatac ccggctttca cgaatggtta
     2641 ctcaaatcat agccgaaaaa gcccctttat tcgaaaaagc ttctattgat gaacattatc
     2701 tcgatcttac aggaatggaa cgttactttg gatgctacaa atgggcacat gaacttcggc
     2761 agagcatcat ccatcatacc ggcttgccta tctcattggg actttcggta aataaaaccg
     2821 ttgcaaaaat ggctaccggc gaagccaaac ccaatggaga aaaggaagta ccatccatgg
     2881 aagtaaaaaa tttcctcaat cctcttccta tccataaaat cccgggcatc ggagaaaaaa
     2941 cctatcaaac cctccgttct atggggatag tcaacataca aaccctcagc gccatccctc
     3001 ccgatatcat ggaaaccctc ctgggcaaaa atggaattat cctctggaaa cgcgccaatg
     3061 gcatcgacac tactcccgta atcccctatt ccgaagaaaa atccatagga cacgaaacca
     3121 ccttcgatca agataccata gatgtgaaca aaattacaaa taccttactg cgcatgaccg
     3181 aacgcctaac tttcgaactg aggaaaaaac agaaactcac ctcctgtgtt accgtaaaga
     3241 tacgttatgc caacttcgat acgcacagcc ttcaaagaca aatcccttat acttcgctcg
     3301 accatgaact tttccccgtg gtaaaaaacc tgttcgaaaa actctatcaa cgacgtatgc
     3361 tcatccgctt ggtaggtgta cgcttcagcc atctcatcca tggatttcat cagctcgatg
     3421 tcttcgatcc ttcgccccgc ctcatcgatc tgtataaagc catggaccac atccgattgc
     3481 gttacggtcc caaagccatc cgccggggaa gcactttcga ataggaaccc ttatcccgcg
     3541 gtttttaaaa taaattgtcc atatttgacg caaattcatc aactccgatt tagagcccat
     3601 attagaaaaa aaactgttta gaattgatct aaataattat ttttgcagtc ggaaacaaca
     3661 ttaagatatt accaatgaaa ttgctgtatt tatgcagatt atgctacagg gattggaagc
     3721 atgaagggaa aatggaatgg catccacaca ggaagacatc cttgatactg ttaattttct
     3781 tacaaaatgc tacgtctata tctgctcaag gtaaaaacga aaataatttt cttacttttt
     3841 ctgcctctta tgttggagaa ggttttgcca atttttctgg aggaataaaa acaggcctta
     3901 cctatttggg gctggtttca ttgaatactt attttgatgc ttcaaaagca catctatggg
     3961 aaggtggtgg cttttttctg agtttatata atacacatgg gggtacgcca gccactgatt
     4021 ttgttggtga ttatcagggt gtttctaaca tagaggcagg caatcacact tatttgtatg
     4081 agctctggta tgcacagaaa ttcagaaaag tgaaaggcat cattggcatg caggatctaa
     4141 atgtaaatta cgctgccagt caatacggga attcatttat caatagttca tttggaattc
     4201 cttccacctt tgcctataat atttcttcac caatttttcc actcacagca ttgggttttc
     4261 atttagaatg gcagccgact gaacatttca ggtggaaagc agccattttt gatggaacgc
     4321 cagaaggctt ggaaaatttt catttaaaat ggaaattgaa caaacaagat ggatttttga
     4381 gtattactga aattgaatgg actaaaaact ggaagggaaa tcattcagga agctataaag
     4441 tcggtgctta ttttcataat agtcaggata gcttgtctca gtcacaagaa aatggtggat
     4501 tttactttgt aggagatcag acaataaatg aacacattgg aatattttct caaatagccg
     4561 taagtccatc atcaaaaaat tcaaatcctg tttatgctgg tttaggttta aattacaaag
     4621 gaattatcag taaaagacag aaagatattt tagggttggc agtggcttat gcagggctga
     4681 aagatagagc cgatgaaaca gccttagaaa caatgtatca ttttataatc aatgagcata
     4741 ttcctttaat accagatttt cagtatatta taaatccagc tggacaggat attgctttaa
     4801 aaaattcctt ggtaggtttt ttaagaatgc gatttgattt ttaaagttca ttgaaaaatg
     4861 gaagaaacca ataattttaa gcggctttca gatttatcac caggtgatga agcaattatt
     4921 gtgaaagtgc tggggcatgg ctctttcagg agaagacttg ccgaaatggg ttttgtgaga
     4981 ggcaaaaaag taaaggtgat taaaaatgct cccctgcaag atcctgttga atacgaaatt
     5041 atgggatata acatttcgtt gaggcgaagc gaagcagaat tgattcaggt gatttctgtt
     5101 caggatgctg cagcatttac aaggaacaaa cattttaatg gaacgattga tgaagaaact
     5161 ttaaaagttt cggctcttga aaaggaaaaa tctattcatg ttgcattggt tggcaatccc
     5221 aacagtggaa aaacaacatt gtttaatcga atttctggtt cacacgaaag agttggaaat
     5281 tatggcggtg taacagtgga tgtgaaagaa gccgaagtaa agaaaaacgc ttatgccatt
     5341 aagctggttg atttacctgg aacttattcc atcacagaat tttctcctga agaactttat
     5401 gtcagagttt atttgacgga aaaaatgccc gatatagttg tcaatgtggt cgatgcttcc
     5461 aatctggaac gcaatttatt tttgaccact cagctgattg acatggactt gaaagttgtg
     5521 attgctctga atatgtatga tgaactccaa aaaaaaggtg ataggcttga ttacaaatat
     5581 ttagggaaaa tgcttggaat acctattgtg ccaactattg ctaccaaagg tgaaggcatc
     5641 gaagaattgc tgcaaaaatt aattgatgtt tatgaagata aagatcccat tgtcaggcac
     5701 atccacatta attatggatc tatagtcgag aaagccatca aacaaattca ggatgtgatc
     5761 tggcaaaatc cagccattac ggataaatta tcttcccgtt atctggctat caaacttttg
     5821 gaaggtgata aaaccattct ctccgaactg gaaaaatatg acaattatcc tcaaatcgaa
     5881 tctactacga ggcaagctat tcaaagcctc gaaaaagaat atggcgatag ttgcgagacc
     5941 gttattactc aactaaaata tggattcatc gaaggagcct taaaagaaac ctatcatcta
     6001 tcgaagcaaa aacataagga gaagcaacaa actttagata atttgtttct gcataagttc
     6061 tggggttatc ctatttttct gttcttcgta tggttaatgt ttcaatcgac cttcagcctt
     6121 ggaaaatatc ctacccaatg gctggaaagt ggaattaact tactgggtag ctggctgggg
     6181 aggataatgg tcccaggacc attgtgtgac ttattggtga atggaatcat tggtggagta
     6241 ggaaatgtgc tcgtcttctt tccgaatatt cttattttgt ttttcttcgt ttccatcctc
     6301 gaggatacag gctatatgtc gagagcggca tttttgatgg ataaattgat gcataaaatt
     6361 ggattgcatg ggcaatcatt tattccactg gtaataggat ttggctgcaa tgttccggct
     6421 attttggcca cgcgaacatt aaaaaatcgt aaagaccgaa ttttaaccat tatcattatt
     6481 ccgtttatgt cttgcagcgc tcgattgcca gtttatctgc tgctcatttc ggccatcttt
     6541 ccaaaaaatc aagcgctcat tctattttca ctttattcga ttggaatttt tatggccatt
     6601 atcacggcat tgttcatgaa acggacagtt ttcaaagaag aagagtcgcc ttttgtaatg
     6661 gaattgcctc cctatcggat accaactcta aaaaccatta acattcacat gtgggataaa
     6721 agtcgggaat atttgcgaaa aataggaaat attatcctgg tagcttccat tatcatctgg
     6781 gcaatgggct attttcctcg tcatccagaa attgataaaa aatatgaaga acaaattttg
     6841 tctgtacaaa acaatactac tttaagtaaa ggtgaaaaag aaaaaacggt taatcaattg
     6901 caaacgacta agatggcaga attacagcaa aagtctatta ttgggcaatt gggtaaagtg
     6961 gtagagcctg caattcgtcc cttaggcttc gattggaaaa tgggcattgg attattcaca
     7021 ggattagctg ctaaagagtt ggtaattagt acaatgggtg tgttgtatca agttgataat
     7081 tccggagata attctgtttc tttacaagaa aaattaaaaa atgaggttca tacagcaggt
     7141 aagttaaaag gagaaaaagt ctttacccct ttggttgctt ttgcttttat ggtttttatt
     7201 ttgctgtatg ttccatgtgt agcaactatt gctgctatga aaaaagaagc aggctggcaa
     7261 tgggctgttt tcggagtagt ttacaataca ttggtagcct ggctgatggc ttttctgatt
     7321 taccaaattg gaaattgtat agtatagttt aaaaatgcta ataaacaacc acattaatct
     7381 tcattacaaa gctgttttat ttcaaactat tatttcgcaa tattttagat atctgcttgc
     7441 atactaaatt gattgatttt gatgcaaatc aatgtaaaac aaacaaataa aaattatttg
     7501 agatataatt tacaatactt tctgcaaata ataatccagc aaaagtttga acattttgcc
     7561 gtgattggga tatttcagat ggagtagttc gtgcaagatg attttatacc ttacctcttt
     7621 cggctcatca agcaatattt cgttgaaact caatcgacct ttgctcgagc aacttgccca
     7681 tttccttttc atggccctga tatgaatggc attgggcttt actcccactt cgtcggccaa
     7741 agtcagcact tcgtttttta attcttcgtg cgtggtcatt ttaaattttg acatttaaca
     7801 atttcataat ttgctgagcg gtttccgaaa tttttttact atcggtaatt cccgaatgaa
     7861 caagtacttc gtaaagcttt tgtctgattt ttaggccgtg tttgttgctc ttgtgccagt
     7921 gcggatattg tgcaaatact ttttccatct cgttggccat cgaagtagca ttttcaaaac
     7981 cggatctgtc taatatccaa taaatactga aaatttcgct tttcattttt ttctcttctt
     8041 gttccttttt ggccgtaacg atttcttcaa tgattttttt cagcacttcg agcgtttccc
     8101 gagtagtttt ttgcctgtcc tgaaaaagtt ttgccagcat ttcggctttt tcggcaatcg
     8161 atttcagata gggagccgta tcgccttcct gtgctacggt tcgctctata cttttaataa
     8221 gattgaaaac tttttctata tcggaagctt tgcttttttc aattttttcc agcgtttctt
     8281 cattaatttc gaatatatca atggtaggtt gaatttctcc gcttttcgta tgctttttta
     8341 ccagttcaat cgtttttcgg gtaaaatctt tcttgatgtc gatgccacga tcgtaatttt
     8401 ccctcagaat ttggtacat
//
LOCUS       DAOORU010000105         8363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_10520_length_8363_cov_5.336062, whole genome shotgun sequence.
ACCESSION   DAOORU010000105 DAOORU010000000
VERSION     DAOORU010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8363
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10520_length_8363_cov_5.336062"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..239
                     /locus_tag="PLF20_07280"
     CDS             90..239
                     /locus_tag="PLF20_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80836.1"
                     /translation="MNTSALILMISTWTIVTCFAVYFFIKILTAKKLDEPDSYLDNDD
                     QTGNG"
     gene            412..1557
                     /gene="wecB"
                     /locus_tag="PLF20_07285"
     CDS             412..1557
                     /gene="wecB"
                     /locus_tag="PLF20_07285"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOX80837.1"
                     /translation="MIRIVTIIGARPQIIKAAAISRTIRDFFTETIQEIIVHTGQHYD
                     EKMSQVFIEQLQIPTPHYNLGIGSASHGIQTARMIEKIENVLLTEKPDFVILYGDTNS
                     TLAGAVAGAKIHIPVAHVEAGLRSFNKLMPEEINRIVCDHCSTLLFSPTATGFHNLVR
                     EGFLPDTLPPYHINNPKIYHCGDVMYDNCLYFSDMAERNSQILQQYGLNPGKFILITI
                     HRDSNTDQPQRLESIFRSLIRLTENTHLPVVLPIHPRTSKVLKSKLQLDPYKNLQHQP
                     NILIIPPASFFDMILLEKNAALILTDSGGVQKEAYFYRKPCVILRAETEWKEIIETGA
                     GALADANEEQILTKSIEYLKNPPRNFPPIFGDGLTAHFILNEIVATE"
     gene            1694..2047
                     /gene="rpsF"
                     /locus_tag="PLF20_07290"
     CDS             1694..2047
                     /gene="rpsF"
                     /locus_tag="PLF20_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOX80838.1"
                     /translation="MNNQYETVFIMTPVLSEDQMRETVKKFHDLLVEKGAEIVHEENW
                     GLRKLAYPIQKKSSGFYQLFEFKAEGSLINELETAYRRDERIIRFLTVLLDKHAIAYN
                     EKKRTNKNDIPTLND"
     gene            2067..2336
                     /gene="rpsR"
                     /locus_tag="PLF20_07295"
     CDS             2067..2336
                     /gene="rpsR"
                     /locus_tag="PLF20_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOX80839.1"
                     /translation="MAQGESEIKYLTPIAVETKKKKYCRFKKSGIKYIDYKDESFLLK
                     FVNEQGKILPRRLTGTSLKYQRKVAQAIKRARHLALLPYVADLLK"
     gene            2350..2799
                     /gene="rplI"
                     /locus_tag="PLF20_07300"
     CDS             2350..2799
                     /gene="rplI"
                     /locus_tag="PLF20_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOX80840.1"
                     /translation="MEVILIQDVPKLGYTDEIVNVKNGYALNYLIPKGYAIMANPSNR
                     KVLAENLKQKAFKQEKVQNEALELAKKLDGLLIRVGAKASSSGKIYGSVNALQIAEVL
                     KNQHHIEVDRKRILIDGDSIKELGTYTATINLHKDVKATIQFEVFAE"
     gene            2840..3622
                     /locus_tag="PLF20_07305"
     CDS             2840..3622
                     /locus_tag="PLF20_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOX80841.1"
                     /translation="MAGFLSMLSHQQIAFIRKLKEKSFRYQEGVFLAEGEKLVTDLLQ
                     SGCMPMEIYFIESYKGPLTLHPRAKAISSKEMEGISQLKTTSTISALFPIPSLPQLNT
                     IIFKDEWGIVLDDISDPGNMGTLLRTADWFGIRYIVCSSTTVDVYNSKCVQASMGSIA
                     RTYVYYLPLDEFFATLPNEVKIFGAQMNGHSIYTTNFPKGGLLLLGSESSGIHPEYEH
                     FIHKSVAVPAAKHNLKQGPESLNVAIAGSIILSEINQQHFSA"
     gene            3666..3914
                     /locus_tag="PLF20_07310"
     CDS             3666..3914
                     /locus_tag="PLF20_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80842.1"
                     /translation="MLKLILIAFTLLIIPAAGIAIKMFFIKGTKFKKTRASSLPGENL
                     SCTCGGEGSYQEYIGKTVALSSFIQLFWRADSQIPGFE"
     gene            complement(3865..4893)
                     /locus_tag="PLF20_07315"
     CDS             complement(3865..4893)
                     /locus_tag="PLF20_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOX80843.1"
                     /translation="MIKTLRTQSLLSYLALSLLLMSCSRKPEKTIITGEAQGTYYQVT
                     YLDVAKRNFSKEIDSLLHAFDRSVSLWDDSSIICRINRNETGVKTDSVFRFVFTQSME
                     VAKRTDGAFNPCVGPLVKAWGFHRKQGKMPDSLLIIELLKHIDYRNIWLQGDSVVKSD
                     TSIELDFNAIAQGYSADLLASFLRQKGITSYLVDIGGEVVTGDPKPDGNQWRVGIEKP
                     SDSASSEREIEAVVEIAHKAIATSGSYRKYRIENGRRYSHTIDPKTGYPVNHNLLSVS
                     VVAKDGITADAYATAFMVMGAEKAWRFIRDNKDLGLEAYFILDENKSNFRIIQTPGFV
                     SLLSKTTE"
     gene            4971..5144
                     /locus_tag="PLF20_07320"
     CDS             4971..5144
                     /locus_tag="PLF20_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010263415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOX80844.1"
                     /translation="MDSGIYKCGLCEYMYRPQKGNPENGIAPGTPFDELPDDCICPRC
                     GNPKTYFYSTDDE"
     gene            complement(5123..5884)
                     /locus_tag="PLF20_07325"
     CDS             complement(5123..5884)
                     /locus_tag="PLF20_07325"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOX80845.1"
                     /translation="MTNQEERIRTAAEFIKKSKYLVTFTGAGVSVESGVPAFRGQGGL
                     WTKYDPKVLDIDYFMKFPEKSWPVIIEIFYQYMGKAKPNAAHLILAKWEKKGLLQMII
                     TQNIDNLHYEAGNRNIIEFHGNTRNLICLECRKIYKVDKNLLENIPPRCSVCKSILKP
                     DFVFFGEAIPTEAYSASIQAAQKCDVLIVIGTTGEVMPANLIPGLAKNNGARIIEINP
                     HISEHTQVVTDVFLPIKASEAMMALDEAIYSSSVE"
     gene            6029..8218
                     /locus_tag="PLF20_07330"
     CDS             6029..8218
                     /locus_tag="PLF20_07330"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOX80846.1"
                     /translation="MKKFYYLVFLVVLVTLSKSLHSQTKELTLDAILKDRGLATKSSY
                     WITPLNDGKNYLKKENDTLKIYDYKTGAYVGYLIALKDLLDAQGEKISMEDFSLSNDG
                     KKMLIATAIEPIYRHSYYAKYYIYDLQQKKLSALSDKGQQRLAEFSPDGSKVAFVREN
                     NLFIKNLDDGSEMPLTTDGKINEIINGTTDWVYEEEFGFTKGFFWSPDGSKIAYYRFD
                     ETRVKQYCFPKYGKLYPENYCYKYPKAGEENSIVDVYVYNIEEKKALKLNTGSENDQY
                     LPRMQWAGNSGMLAIQRLNRLQNHLEILLYNPSSALGNIIYSEENKYYIEITDDLMFT
                     PDGNQFFITSQGNGYNHIYLHSTDGRLNKQLTIGEWEVDHIIGYDAIRKLVYFSAAYS
                     SPLNREICTVDTKGHLKIIAGQPGNNTASFNPNYTYFLKRWSDANTPHIMSICDARGK
                     ELRILENNAGFTEKAKEYQLQPVEFFSFTTSYGVTLDGWMIKPPHFNTQQPYPVLVYL
                     YGGPGSQTVTNYWEGGRLWYQMMALHGIVVVSVDNRGTGFRGEEFRKMTYSQLGKYET
                     DDQIELAKYLIRSGIADSTRIAIFGWSYGGFMSSLCMTKGSDYFTTGIAVAPVTNWRY
                     YDNIYTERYMGLPQSNSQGYDDNSPIHYTDKLKGNYLLIHGTADDNVHFQNSVEMVSA
                     LVNANKHFQTMYYPDSNHGIYTGKNTTYHLYSLMTEFLYEHLLLKQN"
BASE COUNT         2605 a         1697 c         1574 g         2487 t
ORIGIN      
        1 tttattctcc ttacattgtt tattggtctc ggtgtcatgg ttcatctggc ttatcgaaat
       61 cgtaaaaaat accataaaat ataactctta tgaatacctc ggcattaatt ctcatgatat
      121 ctacctggac aatagttact tgttttgcag tatatttctt catcaagata cttactgcaa
      181 aaaagctcga cgagcccgat agttatctgg ataatgacga tcaaacaggc aatggataac
      241 agagacgggg gagacgaggc gacagggaga ttggtggatg gtgattggtg agtggaatgt
      301 ttgtaatttg gaatttggta gttgtaagtt aaaaaaatgg agagatagaa attttacttt
      361 actttgcctc attcaagaaa tggtgtaatt atcggctaag ttttcaagat catgataaga
      421 attgtaacca ttattggagc aaggccacaa ataataaaag ctgcggctat ttcaagaact
      481 ataagagatt tttttacgga aaccattcaa gaaatcattg tccacactgg ccagcactac
      541 gacgaaaaga tgtcgcaggt ttttatagag cagcttcaaa ttccaacccc acattacaat
      601 ttaggaatag gttctgcttc gcatggcatt caaactgccc gaatgataga aaaaatcgaa
      661 aacgtgcttc ttaccgaaaa acccgatttt gtcattttgt atggagatac caattcgact
      721 cttgcaggag ccgtagctgg agcaaaaatc catatccccg tcgctcatgt tgaagccgga
      781 ttgagatcat tcaacaaact catgcccgaa gaaatcaatc gcattgtttg cgatcattgc
      841 agcaccttgc ttttcagccc cacagccacg ggtttccata atctcgtacg cgaaggattt
      901 ctgcccgata ctttacctcc ttaccacatc aacaatccta aaatttatca ttgtggtgat
      961 gtcatgtatg ataattgcct gtatttctct gatatggccg aaaggaacag ccaaatttta
     1021 caacaatatg ggcttaatcc aggaaaattt attctaatca ctattcatcg cgatagcaat
     1081 accgatcagc ctcagaggct cgaatccata ttcagatcgc tcatccgctt gacagaaaac
     1141 acccatttgc ctgttgtttt gcctatccat ccccgaacat ccaaagtctt gaaatcgaaa
     1201 cttcaactcg atccctataa aaatcttcag catcaaccca atatccttat tattccacct
     1261 gcttcctttt tcgatatgat tttactcgaa aagaatgcgg ctcttatttt gaccgattcg
     1321 ggaggagtac aaaaagaagc ctatttttat cgaaaacctt gtgtaatact tcgagccgaa
     1381 accgaatgga aagaaattat agaaacaggt gcgggggctt tggctgatgc caacgaagaa
     1441 caaatcttaa ccaagagcat agaatatttg aagaatcctc cacggaattt tcctcccatt
     1501 tttggcgacg gcctgactgc acattttatt ttaaatgaga ttgttgctac ggaataagct
     1561 cctaaatcca tcctgaatgc tgaactttag ctaaatttta cgattttttt tgtgcaattt
     1621 gtaagaattt aataattttg ccatccaatt taataaaaaa tttattcagt ctaattaatt
     1681 taatttcaat taaatgaata accagtacga aaccgttttc attatgactc ccgtcttgtc
     1741 tgaagatcag atgagggaaa cggtaaagaa gttccacgat ttactggtgg aaaaaggtgc
     1801 tgaaattgtg cacgaagaaa actggggttt gcgcaagctt gcctatccaa ttcaaaaaaa
     1861 atcatcgggt ttttatcagc tcttcgaatt taaggccgaa ggcagcctga ttaatgaatt
     1921 ggaaaccgcc taccgacgtg atgaacgtat tatacgtttt ctcacggtat tgctcgataa
     1981 acacgctatt gcttacaatg aaaaaaagcg taccaacaaa aacgacattc ctacattaaa
     2041 cgattgaaca ctaaaaattg aacattatgg cacaaggtga aagtgaaatc aaatatttga
     2101 ctccaatagc ggttgagacc aaaaagaaga aatactgccg tttcaaaaaa agcggtataa
     2161 aatatattga ttacaaagac gagtcatttt tgctgaaatt tgtcaacgaa cagggaaaga
     2221 ttttgccgcg caggctcacg ggtacttcct tgaaatacca aagaaaagtg gctcaggcca
     2281 tcaaaagagc ccgtcatctt gcacttcttc cctatgtagc tgatttactc aaataaggag
     2341 gaaaaaagta tggaagttat tttgattcaa gacgtaccta agctgggcta taccgacgaa
     2401 atcgtaaacg tgaaaaacgg ttatgcactt aattatctca tccccaaagg atacgctatt
     2461 atggcgaatc cttctaatcg taaagtattg gccgaaaatc ttaaacaaaa agcctttaaa
     2521 caggaaaagg ttcaaaacga agccctcgag ttggctaaaa aattagatgg ccttctcatc
     2581 agagtgggtg ccaaagcttc gagcagcggt aaaatttatg gttcggtgaa tgcattgcaa
     2641 attgccgaag ttcttaaaaa tcaacatcat atcgaagtag atcgcaaacg catcctcatc
     2701 gatggggatt ccattaaaga actcggaaca tacacggcaa ccataaatct tcataaagac
     2761 gtcaaagcta ctattcaatt tgaagtcttt gcagaataat ttgtttggct ttcaataatt
     2821 ggtcccgtca tgaccctcca tggcgggttt tttatctatg ctttcacatc aacaaatagc
     2881 tttcattaga aaactcaagg agaaatcctt tcgctatcag gaaggagttt tcctggcaga
     2941 aggagaaaaa ttagttactg atttgcttca gtcgggatgc atgcccatgg aaatttattt
     3001 tatagaatca tacaagggtc cattaacctt gcatcctcgg gcgaaagcca tttcaagcaa
     3061 agaaatggaa ggaatttcac aactgaaaac tacctcaacc attagtgctt tgttccccat
     3121 tccatcgctc ccacagctta atacgataat ttttaaagac gaatggggca ttgtactgga
     3181 tgatatcagc gatccgggaa acatgggtac attattgcga actgccgatt ggtttggcat
     3241 acgttatatc gtttgttcgt ccactaccgt ggatgtatac aattcaaaat gtgttcaagc
     3301 tagtatgggc tccattgctc gtacttatgt ttattatcta ccgctcgatg agttttttgc
     3361 tacccttcca aacgaagtta aaattttcgg cgctcaaatg aatggccatt ctatttatac
     3421 gacgaatttt cccaagggag gtcttctttt attaggaagc gaatcctcag gcattcatcc
     3481 cgagtatgag cattttatcc ataaaagtgt agccgtcccc gccgccaagc ataatttgaa
     3541 acaagggcca gaatctctta atgtagcaat tgctggatcg attatacttt ccgagattaa
     3601 ccaacaacat ttttcagctt gatttgctgc aaaaattaca aaatgaccga ttttgaaaca
     3661 cggtgatgct caaacttatt ttgatagcat ttactcttct aataattccc gctgcgggta
     3721 tagccatcaa aatgtttttc atcaagggaa ccaagttcaa gaaaacacgt gcctcgtcgt
     3781 tgccgggcga aaaccttagc tgcacttgtg gaggcgaagg ttcataccaa gaatatattg
     3841 gcaaaacagt agctctttca tcctttattc agttgttttg gagagcagac tcacaaatcc
     3901 cggggtttga atgattcgga aattcgattt gttttcgtcg agaataaaat aggcttcaag
     3961 tcctaaatct ttgttatctc taataaatct ccatgctttt tcggctccca ttaccataaa
     4021 tgctgttgca taagcatcgg ctgtaatgcc atcttttgcc acaacactta cacttaacaa
     4081 attatgattg acaggataac ctgttttggg gtcaatcgta tgagaatatc gccttccgtt
     4141 ctctatacga tatttcctgt aacttcccga agtagcaatg gctttgtggg ctatttccac
     4201 cacggcttca atttcccttt ctgagctcgc actgtcggat ggtttttcta ttccaactct
     4261 ccattgattt ccatcgggtt tcggatctcc cgtaactact tccccgccaa tatcaaccaa
     4321 ataggaagtg attccctttt gccgtaaaaa tgaagccagc aaatcggcag aataaccttg
     4381 tgctatggcg ttgaaatcga gttctatgct tgtatccgat ttcacaacac tatcgccttg
     4441 caaccagatg ttccgataat cgatatgttt caacaattca ataatcaaga ggctatcggg
     4501 catttttccc tgcttgcgat gaaatcccca agccttcacc aacggcccta cgcacggatt
     4561 gaaagctcca tcagttctct tggctacttc catcgattgg gtaaacacaa agcggaaaac
     4621 actatcggtt tttacacctg tttcgttacg gttgatacgg caaatgatgc tactatcgtc
     4681 ccataacgag accgaacggt cgaaggcatg caaaagggaa tctatttcct tgctaaagtt
     4741 cctcttcgca acatctaaat aagttacctg ataatatgtt ccttgagcct caccggtgat
     4801 gattgtcttc tcaggtttgc gactgcatga catcaataat agcgataagg cgagatatga
     4861 caaaagagac tgagttcgaa gcgttttaat cataatttct cgaatgatta acatttatcc
     4921 gcgtgcaaaa ttcggtattt ttgctcgaga tttaaatttt gaagcgtatt atggattctg
     4981 gtatttataa gtgtggattg tgcgaatata tgtacaggcc acagaaaggc aatcccgaga
     5041 atggcattgc accgggtacc cccttcgacg aacttcccga cgactgcatt tgccctcgtt
     5101 gtggcaatcc gaaaacatat ttttattcaa cagatgacga ataaatggct tcatcaagcg
     5161 ccatcatggc ttcagaggcc ttaatgggaa ggaaaacatc ggtaacgacc tgtgtgtgtt
     5221 cagaaatgtg tggattgatt tcgataatcc ttgctccatt attcttcgct aacccaggaa
     5281 taagattagc gggcataact tcgcccgtag tgccaataac aatcaataca tcgcattttt
     5341 gcgctgcctg tatagaagca gaataggctt cagtgggaat agcttcgcca aagaatacaa
     5401 aatcgggctt taatatagat ttgcaaactg aacatcgggg aggaatattt tcaagtaaat
     5461 ttttatccac tttataaatt ttcctacatt ccaagcaaat caaatttcgt gtattgccat
     5521 gaaactctat gatgtttcga ttaccggcct cataatgtaa attgtcgata ttttgtgtaa
     5581 taatcatttg gagtaaaccc tttttctccc attttgctaa gataagatga gcagcattgg
     5641 gcttggcttt acccatatat tgatagaata tttcgatgat tacaggccaa ctcttttctg
     5701 gaaatttcat aaaataatcg atgtctaaga ccttgggatc atatttagtc cataaccccc
     5761 cttggcctct gaaggctggc actccacttt ctacacttac tcctgcaccc gtaaaggtta
     5821 ccagatattt cgactttttg ataaattcgg ccgcagttct tattctttcc tcctgattcg
     5881 tcatattcta tttttttatt atttcgaaag ttactcccga ttataattct actttctcat
     5941 caagaaatat agaaaataat ctgtcttcat tgaaaatccg cgaaaaatca ataattttga
     6001 ccacacatat ttcttaaatg caaatatcat gaaaaaattt tattatcttg tgtttttggt
     6061 ggttttggtt accttatcga aatctctaca ttcgcaaacc aaagaactta cactcgatgc
     6121 tatattgaag gaccgaggtt tggcaaccaa aagttcttac tggattaccc ctttaaacga
     6181 tggaaaaaat tacctaaaaa aggaaaacga cactttaaag atttatgatt acaaaactgg
     6241 ggcttatgtt ggctatctta tagctctcaa agacctgctc gatgcgcaag gcgaaaaaat
     6301 ttcgatggaa gattttagcc tcagtaacga tggcaaaaaa atgctgattg caacggcaat
     6361 tgaacccatt taccgacatt cttattatgc aaaatattat atttatgatc tacagcaaaa
     6421 aaaattgagt gctttgtcgg acaaggggca acagcggctg gccgagtttt cacccgatgg
     6481 cagtaaggtt gcttttgtta gagagaacaa cctgtttatt aaaaatctgg acgatggaag
     6541 cgagatgcca ttgacaaccg acggaaaaat caatgaaatc atcaatggca cgaccgactg
     6601 ggtgtatgag gaagaatttg gttttactaa aggatttttc tggtcgcccg atggcagcaa
     6661 aatagcttat tatcgattcg acgaaactcg tgtaaaacaa tattgcttcc caaaatatgg
     6721 aaaattgtat ccggagaatt actgttataa atatcccaag gctggcgaag aaaactccat
     6781 cgttgatgtc tatgtttata acattgaaga aaagaaagca ttaaaattga atacgggttc
     6841 agaaaacgat cagtatttac ctcgcatgca atgggctgga aattcaggta tgctggccat
     6901 tcagcgcctc aatcgcttgc agaatcatct cgagattctt ctttataatc cttctagcgc
     6961 attaggaaat atcatctata gtgaagagaa caaatactat atagaaataa ccgacgacct
     7021 catgtttact cctgatggga atcaattttt catcacgagt cagggaaatg gttacaacca
     7081 tatttatctt cattcaaccg atggaagatt aaataaacaa cttacgatag gagaatggga
     7141 agtagatcat atcattggtt atgatgctat ccgcaagctt gtttattttt ctgcagccta
     7201 tagcagcccg ctaaatcgtg aaatctgcac agttgacact aaggggcatc ttaaaatcat
     7261 tgctggccag ccaggaaata acacggccag ctttaatcca aattacacat attttcttaa
     7321 aagatggtcg gatgccaata caccacatat catgtcgatt tgcgatgcca gaggcaaaga
     7381 actccgaatc ctcgaaaata atgcgggatt taccgaaaaa gcaaaagaat atcagcttca
     7441 acctgtggag tttttttctt ttacgacttc ttatggagtt actttggatg gatggatgat
     7501 aaagcccccc catttcaata ctcaacagcc ttatccagta ttggtttatc tgtacggagg
     7561 acctggatcg caaaccgtaa ccaattattg ggaaggcgga agattgtggt atcaaatgat
     7621 ggctttacat gggatagtcg tagtttcagt agataatcgc ggaactggtt tcagaggaga
     7681 agaattcaga aaaatgactt atagccagtt ggggaaatat gaaaccgacg accagataga
     7741 gctcgccaaa taccttattc gttcgggaat agccgattct acacgtattg ctatatttgg
     7801 ctggagctat ggaggattta tgtcgtcgct gtgtatgacc aaaggctccg attattttac
     7861 tacaggtata gctgtagctc cagttaccaa ctggagatat tacgacaaca tctatactga
     7921 gcgctatatg ggattacctc agtccaactc tcaaggctac gacgacaatt ctcctatcca
     7981 ttataccgac aaacttaaag ggaactatct gctgatacat ggcacggccg acgataacgt
     8041 ccattttcaa aattcggttg aaatggtttc ggcactggtt aatgccaata aacattttca
     8101 aacaatgtat tatcccgaca gcaatcacgg aatttatact ggaaaaaata ctacatacca
     8161 tctctattca ttgatgactg agtttctcta tgaacatctt cttttaaaac aaaactaaca
     8221 tatcccgaaa acatatttta tcgattcgtt tcgtattcat aaataatgta taaatagaaa
     8281 aatttcacat cttataaatt ttgttttgtt atttagagtc tgtctataaa ctcaaattgt
     8341 taataacttt ttgataacta att
//
LOCUS       DAOORU010000106         8277 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_10659_length_8277_cov_5.990513, whole genome shotgun sequence.
ACCESSION   DAOORU010000106 DAOORU010000000
VERSION     DAOORU010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10659_length_8277_cov_5.990513"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="PLF20_07335"
     CDS             complement(<1..1071)
                     /locus_tag="PLF20_07335"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOX80847.1"
                     /translation="MSLIINEKKLLGINGLGRIGKLTLWYHLIQRKFDGIVVNIGREV
                     GKSLDDLVQIILNDSTYGRLDHFLYGHSGKKCQVEIPDYESQIVFIDGFPIKILNKER
                     SPQNINWRNEKVRIVVDCTGKFLDPTISPAEGKDALCGHLTAGAQKVISSAPFKIKNA
                     STHDLDSFPTLIYGINHTSYDPLRHHIISAASCTTTGLAHMIKPLLENKETSNVLTAS
                     MSTVHAATNTQSVLDSVPAAGATDLRKNRSVFNNIILSTTGAAKTLEMVIPQVTNFGF
                     MADSIRIPTNTVSLIALNITFSSSLDEKGMPSINANSIKEIYKTAANGNQKGLLVFST
                     KQNVSADLLGFAAAIVIEGNDIH"
     gene            complement(1103..5158)
                     /locus_tag="PLF20_07340"
     CDS             complement(1103..5158)
                     /locus_tag="PLF20_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOX80848.1"
                     /translation="MFTYLQKDALPEKYSWFLSLSENYWGIHKRTQDFFEELKKPDHN
                     SKKLIDLLTDVCISDFWIYKDLPDKHQVIQFIYEIFDSLLGENLSEENAKRLVYVFLD
                     FFSKNYNSFGEKDKLVFEFIDILKKNYEKRSLSYLSNIGQFKKSLQKAAYNKTTSGPV
                     IDFMRLLIKNNIVFWDTTTRIEEWYRNNSDKMSADYSDVIKSLGKDFFNDYYQKLNEA
                     DSWESLCELAFTFSDIIEEFRSKIENFNKATEQFSYIFYLLQCPGMAFHKDFLLMDLN
                     RAIKRISKELNEDECIESIDKLFSLFSNFNKTHMSLILDSILALGKEIININNKKIIH
                     YLEDRIIEFGFVSPGITYLDNDWELKVDPNHVKNTRVWLELIEFNPEMMKKLLSALII
                     NLRIGGIFIYDSDLFQKDVSKLLNSKISPIYKQIKQLCRIFPVYYNEIGAEGLIREIS
                     TKIDEISYRNDKLIHFLRKQIHTEGNSSHVQITMEIIRFWYDLDKKRLGKIIPPNVLD
                     TIEIKGKWVKGVHQVLRKISQFSGCTLDELLTKEQDDIEIFLSSINHDDKDDLERVAL
                     IFELYRLLKEKYRFETTKIIAVLKRYHFITNDDIESLEAHLNQNDPIGALKEIYSLMI
                     KLNEIIFDPEISEGWESIFYKRHVAAGIPSMYGYYRETKFEALGLTFRLEKVASQLVS
                     TIISEINTEYFTAKTLKKIYSILQLLREGLSLDGIYDQGFDSNLKIFQNSLTSESFTI
                     NQYINIFQFMEMNIKEIINKYFISPYESLLKIIIPQYVPQSNTMSADALNKLIIRKSE
                     IFYRELLSSAFLIQYLDNFIGALLNNLRNLINNLSDTEIRSMLSYDPDMVISPLYHET
                     PALDNQAFIGSKAYFLKKLYLNQYPVPPGFVLSTEVFRRVNLILKLPSLNSEIDQFIK
                     TYVTELENMSGLKFGDTKKPLLLSVRSGAAISMPGAMLTLLNVGLNDEITESLSKCNN
                     FGWTSWDCYRRLLQSWGMAHNLDRNDFDQIMLDYKQKYNVAQKIDFSPEMMREIAFAY
                     KQLLIDNNIYFEEDPFLQLKKAIISVFYSWDTPRAKVYREYMHIAEEWGTAVIVQQMV
                     FGNLHNESGSGVVFTHDVHDPSSGISLSGDFTFLSQGEDIVAGLVNTLPISEKQRKEF
                     YHKSPFSLETKFPKIYCRLKEIAHELIEIHGFGHQELEFTFETSDPKDLYILQSRDMR
                     VMKQNSVEVFATSAKKMQLVGCGIGIGNKVLNGVIVFDSHDLQVLKQESPHKNAILVR
                     PDTVPDDIEMIFECEGLLTAKGGATSHAAVTAASLGKIGVVNCDSMIVFEKEKKCIIN
                     GNTFTSFDLIAIDGNKGLVYKGNYPIKIQEL"
     gene            complement(5161..6159)
                     /gene="gap"
                     /locus_tag="PLF20_07345"
     CDS             complement(5161..6159)
                     /gene="gap"
                     /locus_tag="PLF20_07345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOX80849.1"
                     /translation="MSVKIGINGLGRIGRLVFRLAIETPGIELVHLNDMMDTPLMSHL
                     IKYDSLHGKLNADLSCQQDHLVVNGSRILVTHCEKPDAIPWKHTGVDIVIDASGKFKT
                     STSLQAHLRNGAKKVILTCPALDNSIERTIVMGVNHKEILPSDRIISNASCTTNCVAI
                     MLKVLQDEFGVKKAFMNTIHPLTNNQNIQDGYHTDYRRARSAINNIIPTTSSAIDAIR
                     IIFPEMKDTFDGFATRVPVAECSFVELIAELDKNVEKYIVNQAFLKHSEGNLNAYLEY
                     CSDPIVSSDIHNNRHSAIFDSLATKVLAGDFIQILGWYDNEFGYSARIIDLIKYIS"
     gene            6677..8173
                     /locus_tag="PLF20_07350"
     CDS             6677..8173
                     /locus_tag="PLF20_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005863241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HOX80850.1"
                     /translation="MTVNFEFFLFIVSILLFFSIIAGKAGYRFGIPVLLLFLMVGIVA
                     GSDGLGIEFSSARQAQYIGIIALNIILFSGGMDTRFSEIKPVLKPGIAISIGSTILTA
                     FITGFFIYWFTNYIFDSVRFNLVEALLLASIMASTDSASVFSILRSKGLTITQNLRPL
                     LELESGSNDPIAYMLVIICIELLKNSSIHFGQIMLLFVQQIVVGVLVGLIFAKLVVHI
                     INHIHLDYEGLYSVLLLAVVFFLFSFSTILGGNGYLAVYLGGLLIGNHSFVHKRSTLK
                     FFDGLTWLFQIIMFLTLGLLVNPHELLPIAILGIIIGMFMIIVSRPLSVFAILTPFKK
                     ITFKGRVFVSWVGLRGAVPIIFATYPLIHGIEQAQTMFNIVFFITILSLVLQGSSVPM
                     VARWLGLTAQTTKKTKPQLYEFDMEFPDDIKSAISEMTITEQMLKHGRRLMDIRLPDK
                     ILVVMIKRNDKYFIPRGNTHLEPGDVALLIADNEEVLKETYRHLGVPY"
BASE COUNT         2424 a         1560 c         1482 g         2811 t
ORIGIN      
        1 atgaatatca tttccttcga tcactatggc tgctgcaaaa ccaagtaaat cggccgatac
       61 attttgtttg gtagagaaaa ccaataaccc tttctgattg ccattggctg cagttttata
      121 aatctctttg atgctattgg cattaataga aggcattcct ttctcatcaa ggcttgagct
      181 gaaagtgata ttgagggcaa tcaatgacac ggtgtttgtg ggtattctta tagaatctgc
      241 cataaagccg aagttagtga cttgtggtat gaccatttcg agtgttttag ctgctccagt
      301 tgtcgaaaga ataatattat tgaataccga cctgttcttt cgtagatctg ttgctccggc
      361 tgcagggact gaatcgagca cgctttgtgt attggtagcg gcatggactg tcgacataga
      421 agctgtaagc acattactgg tttctttatt ctcgagaaga ggctttatca tgtgagcaag
      481 tccggtagta gtacagctgg ctgctgaaat tatatgatgt cttagagggt cataactggt
      541 atggtttatt ccataaatga gagtagggaa gctatcgaga tcatgggtcg aagcattttt
      601 aattttaaat ggagcgcttg aaattacttt ttgcgcacca gcagtcaaat gaccacataa
      661 ggcatcctta ccttcggcag gtgaaatggt agggtctaag aactttccgg tgcaatccac
      721 tacgattctt actttctcgt ttctccagtt gatattttga gggcttcttt ctttattgag
      781 gatttttatt ggaaatccat cgatgaaaac tatttgactt tcataatctg gaatttcaac
      841 ctgacatttt ttccccgaat gcccatacaa aaaatgatcg agcctaccgt aagttgaatc
      901 gttgagaatg atttgaacaa gatcatcaag acttttgcct acttctctac caatattaac
      961 gacaataccg tcgaattttc tttgaatgag atgataccat aaggttagtt ttccaatacg
     1021 gccgaggcca ttaattccta ataatttttt ttcattgata attagtgaca taacggttta
     1081 ttttggttta ttacaaaatt atctataatt cctgaatctt gataggataa tttcctttgt
     1141 ataccaaccc tttgtttccg tcgatagcaa tgagatcaaa tgaagtaaaa gtatttccat
     1201 taataataca ttttttttct ttttcaaaaa cgatcatgct atcgcaattt actacaccaa
     1261 tcttccctaa tgatgctgca gtaacggctg catgtgaagt ggctcctcct tttgctgtta
     1321 ataatccttc gcattcaaaa atcatttcta tatcatcggg gacagtatct ggtcgtacca
     1381 agattgcatt tttgtgagga ctctcctgtt tgagtacttg cagatcatgc gaatcgaata
     1441 caatgactcc atttaaaact ttgtttccaa tgcctattcc acatcctacg agttgcattt
     1501 tttttgcgga agtagcaaaa acctcaacag aattttgttt cataactctc atgtctcttg
     1561 actgtaggat gtataaatct ttggggtcgg aggtttcgaa tgtaaattct aattcttgat
     1621 gcccaaatcc atgaatctct atcaattcgt gagcaatttc tttgagtctg caataaattt
     1681 tgggaaattt tgtttcgagc gagaaggggc ttttgtgata aaattccttg cgttgctttt
     1741 cgctgatggg taaagtgttt actaatccag caacaatatc ttcgccttgg cttagaaaag
     1801 tgaaatcgcc ggacaggctg atacccgaag atggatcatg aacatcatgg gtaaatacga
     1861 cacctgaacc agattcattg tgtagatttc cgaaaaccat ctgttgaact attactgcgg
     1921 ttccccactc ttcggcaatg tgcatgtatt ctctataaac cttagctcgc ggcgtatccc
     1981 atgaataaaa taccgaaata atggctttct tcaattgtaa gaatggatct tcttcgaagt
     2041 aaatgttatt atctataagt agttgtttat aagcaaacgc aatctcacgc atcatttccg
     2101 gagaaaagtc gattttttga gctacattgt atttttgttt ataatcgagc ataatctggt
     2161 cgaaatcatt tctatcgagg ttgtgagcca tcccccatga ttgtaaaaga cgacgatagc
     2221 agtcccatga agtccaccca aaattgttgc atttgctcaa actttcggtt atttcgtcgt
     2281 ttaatccaac attcaggaga gttaacatag ctcctggcat agaaatggct gcacctgaac
     2341 gcacactcaa taataatggt tttttggtat ctccaaattt taaacctgac atgttttcca
     2401 gctcagtaac ataagttttg ataaactggt caatttcaga atttaaggaa ggtaatttca
     2461 agatcaaatt tactcttctg aaaacttcgg tactgagaac aaatcctggg ggaacaggat
     2521 attgatttaa ataaagcttt ttaaggaaat aagcttttga ccctataaaa gcctggttgt
     2581 ccaaggccgg tgtctcatgg tagagtggac ttatgaccat atcgggatcg tagcttaaca
     2641 tgctacggat ttctgtatcc gagagattgt tgattaggtt tctcaaattg ttcagcagag
     2701 ctcctataaa gttatccaga tattgaatga ggaaggctga agagagtagc tccctataaa
     2761 atatctccga ttttcgtatt atcagtttat tgagagcatc tgcactcatg gtattcgatt
     2821 gaggtacgta ttggggaata ataattttca aaagagattc ataaggactg ataaaatatt
     2881 tattgatgat ttctttgata ttcatttcca tgaactggaa gatgttgatg tactgattga
     2941 tggtgaaact ttcggaagtg aggctgtttt ggaatatttt taaattggaa tcgaatccct
     3001 ggtcgtagat tccatctaaa ctcaaaccct ctcttaatag ctgcaaaatc gaataaatct
     3061 tttttagtgt tttagcagta aaatattctg tattgatttc ggagattata gtcgaaacta
     3121 attgagaagc cactttttcg agccggaaag tgagccccag ggcttcgaac ttcgtttcgc
     3181 gataataacc atacatcgaa ggaataccag cagctacatg ccttttataa aatattgatt
     3241 cccaaccttc actaatttcc ggatcaaata ttatttcatt caattttatc attaacgaat
     3301 aaatttcttt taaggctcca atagggtcat tctggttcaa atgagcctca aggctctcga
     3361 tgtcgtcatt cgtaatgaaa tggtatcgtt ttaaaacagc aattatcttg gtagtttcga
     3421 atcgatattt ttcttttaaa agcctgtaca attcaaatat caaagccact ctttccaaat
     3481 catccttatc gtcgtgattt atggatgaaa ggaatatctc tatatcgtct tgttcctttg
     3541 tcaataattc atcgagggtg caaccagaga attgggaaat ttttctaaga acttgatgaa
     3601 ctccctttac ccatttccct tttatttcta ttgtatcgag tacattggga ggaattattt
     3661 ttccgagtct cttcttatct aaatcatacc agaaccttat gatttccatg gttatctgta
     3721 catgtgaact atttccctcg gtatgtatct gtttgcgtag aaaatgtata agcttgtcgt
     3781 ttctataaga tatctcgtca atttttgtgg aaatttcgcg tattaagcct tccgctccta
     3841 tctcattgta atacacaggg aaaatacggc atagttgttt aatttgttta tagataggag
     3901 atattttaga gttgaggagt ttggatacat ctttttgaaa aagatcggaa tcgtaaataa
     3961 atataccgcc aatacgcaaa ttgatgatta aagcagagag aagctttttc atcatctcgg
     4021 ggttaaattc gatcagttcg agccataccc tggtgttttt aacatgattt ggatctacct
     4081 ttaactccca atcattgtct aaataggtaa ttcccggact aacaaaaccg aattcgatta
     4141 tcctgtcttc gagataatga attatctttt tattgtttat attgatgatt tcctttccca
     4201 aagccagaat ggagtctaag ataagactca tatgcgtctt attgaaattg gaaaaaagag
     4261 aaaataattt atcgatggat tcgatgcatt cgtcttcgtt caattccttg cttattcttt
     4321 tgatagccct gttgagatcc atcaatagga aatctttatg aaaggccatg ccagggcact
     4381 gcaataaata aaaaatataa gaaaattgct cggtagcttt attaaaattt tctattttgc
     4441 ttctgaattc ttctataata tcggaaaacg taaaagccag ttcacacaat gattcccatg
     4501 aatcggcttc gtttaatttc tgataataat cattgaaaaa gtccttccct aatgatttta
     4561 ttacatcgga ataatcggcc gacatcttat cactattatt cctgtaccat tcttcgattc
     4621 gagttgtagt atcccaaaaa acaatattat tttttatgag tagcctcata aaatcaatta
     4681 caggtccaga ggtcgtttta ttataagctg ctttttgaag actttttttg aactgcccaa
     4741 tattacttaa atagctcaac gatctttttt cgtagttttt tttgagaata tcgataaatt
     4801 caaacaccaa cttatccttt tcgccaaaag aattatagtt tttcgagaaa aaatcgagga
     4861 aaacataaac taagcgtttt gcattctctt ctgataaatt ttcccccaac aaagaatcga
     4921 atatttcata aataaattga ataacttgat gtttatcggg taaatcctta tatatccaga
     4981 agtcacttat acagacatct gtcaaaaggt cgatcaattt tttactatta tgatctggtt
     5041 tcttgagttc ctcgaaaaaa tcctgtgttc ttttatggat tccccaataa ttttcggaaa
     5101 gcgataaaaa ccacgaatat ttttctggaa gagcatcttt ctgtaagtag gtaaacacgg
     5161 ctaagagata tatttaatca aatcaataat tcttgcagag tagccaaatt cattatcata
     5221 ccaaccgagt atctgaatga aatcgcctgc caatactttt gtagccagcg aatcgaatat
     5281 ggctgaatga cgattattgt gtatatcact gcttactatt ggatcggaac aatattccag
     5341 gtaggcatta agattaccct cagaatgttt taaaaatgct tggtttacaa tatatttctc
     5401 gacattttta tccaactcgg cgattaattc gacgaatgaa cattctgcca caggaacacg
     5461 cgttgcaaat ccatcgaatg tgtccttcat ttcagggaag ataatcctga ttgcatcaat
     5521 agcgctcgag gtagtcggaa ttatattgtt gatagcagat cttgcccgac ggtaatcagt
     5581 gtgatatcca tcctgaatgt tctgattgtt ggtgagggga tgaatggtat tcatgaaagc
     5641 ttttttcacc ccaaattcat cttgtaaaac cttcagcatt attgctacac aattggtagt
     5701 acatgaagca ttggatatga ttctatcgga gggtaaaatt tctttatgat tcacccccat
     5761 tactatggtt cgctcgatcg aattatctaa ggctgggcaa gtcaaaataa cctttttcgc
     5821 tccattgcgc agatgtgctt gcagtgaggt gctggtttta aattttccgc ttgcatctat
     5881 cacaatatcc actcccgtgt gtttccatgg aatcgcatcc ggtttctcac agtgcgtaac
     5941 caaaatcctc gagccattaa ctaccagatg atcctgctga caactaagat cagcatttag
     6001 ctttccatgc aagctgtcgt attttattaa atgactcatt aagggagtgt ccatcatatc
     6061 attcaagtga actaattcta tcccaggggt ttcgatggct aaccgaaaaa ccagcctgcc
     6121 gattcttcct aacccattga tgcctatttt aaccgacatg accgttgagt aatgagttaa
     6181 taaccagaat gtaggatata caaacggatc ttcagtatat cagtttttta ctatatctga
     6241 ttcagcaagc aaatttacgc atttattcca tgatgacacg tataaaagaa cctgtatgcc
     6301 gatggaagaa tttggatctc ttttcatgga atttaatcaa tggcatcata ctaatttaaa
     6361 ataggaatac tttacttttg aattaattct attcagctaa tatccacctt ccttattcgc
     6421 ataaaaattt caacggcaat tattcgaaaa gctaaaacat cattttttga aaactgaggg
     6481 aaacaatttc aacttacaaa gctaaatatt gaagataatt cagtaatttt agccctacga
     6541 aaaattttaa ttgttggaag atttttcctt gaatggaata aatttagcaa tgcagtatct
     6601 gtctgatttt gccgctcatt aaattaccat tcatgcttga aagtgaattg atatgaaatc
     6661 cacataaata agttatatga ctgtgaattt tgaatttttt ctttttatag tatccatatt
     6721 gctctttttc agtatcattg cagggaaagc aggataccgg tttggaattc cagtattgct
     6781 actattctta atggtgggta ttgtggcggg gagtgatggt ttagggatag aattcagttc
     6841 agcccgtcaa gcacagtata ttggaatcat tgctcttaac atcattcttt tttcgggagg
     6901 aatggatacc cgtttttccg agattaaacc cgtattgaaa ccaggaatag ccatatcgat
     6961 tggcagtacc attctaacgg cattcatcac tggttttttt atttattggt ttaccaatta
     7021 cattttcgat tccgtgagat tcaatcttgt agaagcatta ctattggcca gtattatggc
     7081 ttcgaccgat tcggcttcgg ttttctccat acttcgttca aaaggcttaa ccattacgca
     7141 aaacctccga cccttgctcg aactcgaaag tggcagtaac gaccccatag cttatatgct
     7201 ggttattatc tgcattgaat tattgaaaaa ttcgtcaata cattttggac aaataatgct
     7261 attattcgtt caacagatag ttgtcggagt cctagtaggc ttgatattcg ccaaactggt
     7321 agttcatatc attaaccata tccatctcga ttatgaaggc ctatattctg ttttgctact
     7381 tgctgtggta tttttcctgt tcagtttttc cactatttta ggtgggaatg gttatttggc
     7441 agtttatctt ggaggattac tcattgggaa tcattctttt gtacataaac gtagcacttt
     7501 aaaattcttc gatggtttaa cctggttatt tcaaatcatc atgttcttga ccctcggact
     7561 tcttgtaaac cctcacgaat tgctgccaat agccatactg ggcatcatca ttggaatgtt
     7621 tatgattatc gtttcgaggc ctctttctgt ttttgctata ttgacgcctt tcaagaaaat
     7681 tacgttcaaa ggtcgtgtat tcgtttcgtg ggtggggttg cgaggcgccg tacctatcat
     7741 ttttgccact tatcccctca ttcatggaat agaacaagcc caaaccatgt tcaacatcgt
     7801 ttttttcatt actattcttt cacttgtatt gcaaggttca tccgttccta tggtcgcccg
     7861 atggctcggt ttaacggctc aaactaccaa aaaaaccaaa ccccaactct acgagtttga
     7921 catggaattc ccagatgata tcaaatcggc tatcagtgaa atgacgatta ccgaacaaat
     7981 gctaaaacat ggccgacgat tgatggatat tcgcttgccc gataaaatac tcgtggttat
     8041 gataaaacgt aatgataaat attttatacc cagaggcaat acacacttag aaccaggcga
     8101 cgttgctctg ctaatcgccg acaacgagga agttttaaaa gaaacctatc gtcacttggg
     8161 agttccttat taattcctct ctcatttttt ataattaccc tcttttcatt accttaaccc
     8221 ccttcctgtt ttccgcattg ggacacccta aaaattttta caaacaattc ggtacag
//
LOCUS       DAOORU010000107         8233 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_10737_length_8233_cov_5.911714, whole genome shotgun sequence.
ACCESSION   DAOORU010000107 DAOORU010000000
VERSION     DAOORU010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8233)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8233)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8233
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10737_length_8233_cov_5.911714"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..359
                     /locus_tag="PLF20_07355"
     CDS             45..359
                     /locus_tag="PLF20_07355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX80851.1"
                     /translation="MGLNIGSMGGVQGSVKFPGLSLYSASKAAVAVMTEVLAEELRPR
                     GIHVNCLAPGSIKTEMFREAFLGVDEAFDPEELAEFISDFAEYGFEFFNGKILPVSVT
                     TP"
     gene            complement(432..1532)
                     /locus_tag="PLF20_07360"
     CDS             complement(432..1532)
                     /locus_tag="PLF20_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80852.1"
                     /translation="MNTINTDNCEAYFLDYYEGRLSKDEIASLRQFLAMHPEWQTKFE
                     DWENIHLPNIQLSFPEKEWLKHPSADEAINISFSNYEYYFIAALEGELTQPQRYSLDL
                     FLKQHPELEKEFDLYALTRLKPQHIPFPDKKQLLHKKKNIPLKYFVPALAAAAAIMLL
                     ILFQPSKHRNYPPASDRPFAQTSTVQEHSLTPLSLPSIIPSTEEKTTRKIQPAAKLTS
                     TSLPQRQVLIPIANMPSKEITNISIHTTPPEVKNIHRIQTAIYMEMIAQQQNNTNEDR
                     PHLLAKIVKPVVGLFQKKSNDEMANRNEEINLWTLAEYTIKGFNAITQKDIEWRTQKD
                     EEGKIVALAIEGENFKIARVKPQKQEETEKEQ"
     gene            complement(1545..2027)
                     /locus_tag="PLF20_07365"
     CDS             complement(1545..2027)
                     /locus_tag="PLF20_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOX80853.1"
                     /translation="MTTAEYNHCVEQHADNVYRFVVKNIGNEQVAKDIVQEVFTKMWE
                     KASTIAYQKSKSYLFTAAYHTLIDTVRYQNRFASLETSPEQAYEPSNYDGIKGILEEA
                     LNRLPPVQKAVVMLRDYEGYSYAEIGEITGLSESQVKVYIFRARQSLKNYLVTIDNLI
                     "
     gene            complement(2109..3896)
                     /gene="lepA"
                     /locus_tag="PLF20_07370"
     CDS             complement(2109..3896)
                     /gene="lepA"
                     /locus_tag="PLF20_07370"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOX80854.1"
                     /translation="MKNIRNFCIIAHIDHGKSTLADRLLELTDTISERQQQQQVLDNM
                     ELERERGITIKSHAIQMNYHYNNEDYILNLIDTPGHVDFSYEVSRSIAACEGALLVVD
                     ATQGIQAQTISNLYLALDHELTIIPVLNKIDMENAMIDEVSDQMIDLLGCSPDEIIAV
                     SAKTGYQVEEILKAIVERISPPQGDPEAPLQALIFDSVFNPFRGIIAYFRVFNGTLRT
                     NDFVKFVATGKEYHADEIGILGLERKPCNELSAGNVGYIISGIKSAKEVKVGDTITHV
                     ERPCDKPIEGFSNVKPMVFAGIYPVDADEYEELRGSLEKLQLNDASLVFEPESSAALG
                     FGFRCGFLGLLHMEIVQERLDREFDMDVITTVPNVSYKVYTTKGEVIDVYNPSGLPDP
                     TQIHHIEEPYIRANIITKAEFVGAIMKLCIEKRGTLVNQTYLTTDRIELIVDLPLGEI
                     VFDFYDKLKSISKGYASFDYHIIGYKEANLVKLDILLNGDSVDALSTLIHRDNAYNFG
                     RRICEKLKELIPRQQFDIAIQAAIGSKIIARETVKALRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKRMRQIGNVEVPQSAFLAVLKLD"
     gene            4188..5003
                     /locus_tag="PLF20_07375"
     CDS             4188..5003
                     /locus_tag="PLF20_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80855.1"
                     /translation="MRKIALVFDFDDTLAPDSTSAFLEWMGVNAQDFWKNRVDPLCSD
                     GWDPVPAYLYSLLKLSNENEEYKITRQCLEEFGSKMKFHKGVMKIFSFLISYFAKHYP
                     EISLEFYIISSGIGDIIRNCRIRKFFSDIWTCDFHYNEEGWAIFPKNIVSFTDKTRYL
                     FQISKGISREDSKADHYAVNRRVSSNGYRIPLQNMIFVGDGLTDVPCFSVIRRHGGIA
                     IGVYDKHRHERWAKAWNFLEEDRIDNLLSADYSLDSDLTRSLIMAIERIADRM"
     assembly_gap    5760..5769
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6053..6673
                     /locus_tag="PLF20_07380"
     CDS             6053..6673
                     /locus_tag="PLF20_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80856.1"
                     /translation="MKSLKINLFLALPIMIILGTSCSKEQVNQQKNQLKTSDTCLIKI
                     ANIDSIGIPHLAIDSSRILNAYQTYMNNYNDDSLNIKYYADSLNFNWYYNDSTDVESL
                     ANRQFYLIIYGHKFQNDEFYGYSKIATELIRIENEVFFNRCNDNKSIGVTYSYECINK
                     GCAAGCEWVYAWHWWIFHGRITNCTCPETPGECEFHMGVKHDLSLF"
     gene            6823..7602
                     /locus_tag="PLF20_07385"
     CDS             6823..7602
                     /locus_tag="PLF20_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80857.1"
                     /translation="MKKNFFIPIITSLILLFLSPTLHSQNPIVIAEKQGSNYKIIYDS
                     VKLIQLIIEDLEFFYKDEHVLITPSKIYQLPFWEPFDSLKTFLKARFNESSHIIGGYT
                     KDLTYKGSFDHPELIGEEILGLILKANNEPKDTSGFKVYALKSKVDSIKIEKIHGQWC
                     LVGYGKYDSADAKAYYFLKKKGKKMILSVKDNILEGMVICSVVNPEYDCNPVLGEYGC
                     ECMEDKGKQKGCDPRIDQRSTSLSGHSSFGMSSLLPSPYPF"
     gene            7672..8223
                     /locus_tag="PLF20_07390"
     CDS             7672..8223
                     /locus_tag="PLF20_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80858.1"
                     /translation="MKQVFLAHTQEKVAIIVLAAVLFSMLSCNKSSKQVAIARLNSKD
                     SSFEFSMDTNRLKALMIEGFDIDRSDSNILLTPTAIYYANIMVSDTHTAPVMAYFKER
                     LPKGSQATITFTTTENKSDTGSIFKTRVILAADAEPIHFDGLTKMEAKTQINGIKISK
                     IRSAYWLEERVFMTNCRWHFSFV"
BASE COUNT         2420 a         1467 c         1631 g         2705 t
ORIGIN      
        1 ctactggaga aaatgggaat atctattacc taaaaattta gggtttggga ttaaatattg
       61 gttccatggg tggggtgcag ggaagtgtaa aatttccggg cttatcctta tatagtgcct
      121 cgaaagctgc agtagccgtc atgaccgagg tgcttgcaga agaactcagg cctcggggca
      181 ttcatgtcaa ttgtctggcg ccaggttcca taaaaactga aatgttcaga gaagcctttc
      241 tcggagtgga tgaagccttc gatcccgaag aactcgcgga attcataagc gatttcgccg
      301 aatatggctt cgaattcttt aatgggaaaa tcctgcctgt ttcggttact acaccttgac
      361 tttatcaact ttttctgata gtgtaatttg tttttactat gtttttgcgg gaaatgaccc
      421 gaaatgagcc attactgttc tttttctgtt tcttcctgtt tctgaggctt aactctggca
      481 atcttgaaat tttctccttc gattgccaga gctacaattt ttccttcttc atccttctgg
      541 gttctccatt ctatatcttt ctgagttatg gcattgaacc ccttgatggt atactcggcc
      601 agagtccata aattaatctc ttcgtttctg tttgccattt catcgttcga ttttttctgg
      661 aatagtccaa caacgggttt cacaattttg gccaataaat gaggccggtc ttcatttgta
      721 ttgttttgct gttgtgcgat catttccata taaatggctg tttgtatgcg atggatattt
      781 ttaacttcgg gaggagttgt atgtatagaa atatttgtaa tttcttttga tggcatgttg
      841 gctataggaa tgagaacttg ccgctgaggc aaggaagtag aagtgagctt tgcagcaggt
      901 tggattttgc gggtggtttt ttcttctgtt gaggggataa tagagggcaa agaaagcgga
      961 gtaagagaat gttcctgaac tgttgaagtt tgggcaaatg gtctatcaga tgctggtgga
     1021 taatttcgat gtttcgaagg ttgaaataat atcaataaca ttatagcggc tgctgcagcc
     1081 aaggctggaa cgaagtattt tagaggtatg tttttctttt tatgtaacaa ttgtttttta
     1141 tcggggaagg gaatatgttg tggtttcagg cgggttaaag catacagatc gaattccttc
     1201 tccagttcgg gatgttgttt caagaagaga tcgaggctgt agcgttgggg ttgggtaagc
     1261 tcaccttcta aggcagcaat gaaataatat tcgtagttgc tgaagctgat gtttatggct
     1321 tcgtctgccg aaggatgttt gagccattcc ttttcgggga atgacaattg gatattgggc
     1381 aggtgaatat tttcccaatc ctcgaatttg gtttgccatt ctggatgcat agccaaaaat
     1441 tgccttaagg aagcaatttc gtctttgctt aatctgcctt cgtagtaatc gaggaaatag
     1501 gcttcgcaat tgtctgtatt aatggtattc atcacttcga gtctttaaat aaggttatcg
     1561 atagtcacaa gataattttt aaggctctgg cgagcgcgaa agatataaac cttcacctgg
     1621 ctctcgctca gtcccgtaat ttcaccaatt tcggcatagg aatacccttc gtaatctcgc
     1681 aacatcacga cggctttttg taccggaggt aatcggttta aggcttcttc caatattcct
     1741 ttgatgccgt cgtaattaga aggttcgtaa gcttgctcgg ggctggtttc caagcttgca
     1801 aaacgattct gatatctcac cgtgtcgatc aatgtatgat aagccgctgt aaacagatag
     1861 gattttgatt tttgataagc aatagtcgag gccttttccc acattttcgt aaacacttcc
     1921 tgaacgatat ccttggccac ttgttcattt ccaatatttt tgactacaaa ccggtaaaca
     1981 ttatcggcat gttgttccac gcaatgatta tattcggcag tggtcatgag aattttggga
     2041 tgttcgaatg attaactaaa gacggaggaa tgctggaaaa gttacaaaaa atgtagaatt
     2101 cttgcttatc agtccagttt taaaacagcc agaaaagccg attgaggaac ttcgacgttt
     2161 ccaatctggc gcatacgttt tttccctttc ttttgttttt cgaggagttt tcttttacgt
     2221 gtaatatcgc ctccataaca tttggcggtg acatccttgc gcaaggcttt tacggtttcg
     2281 cgggcaatga ttttcgaacc tatggctgcc tgaatggcga tatcgaattg ttggcgggga
     2341 atgagttctt tgagtttttc gcagatacgg cggccaaaat tataggcatt gtcacggtga
     2401 atcagtgtgg agagtgcatc taccgaatcg ccgttgagca atatgtcgag ttttacaaga
     2461 ttggcttcct tgtatcctat gatgtgatag tcgaatgagg cataaccttt cgaaatggat
     2521 ttcaacttgt cataaaaatc gaatacaatt tctcccaagg gaagatcgac gataagctcg
     2581 atgcggtcgg ttgtaagata agtttgattg accagtgttc cgcgtttttc gatgcagagt
     2641 ttcatgatag ctccaacaaa ttccgctttc gtaatgatat tggcgcggat gtaaggctct
     2701 tcgatatggt gaatttgtgt gggatcgggt aaaccgcttg gattgtaaac atctattact
     2761 tcgcccttgg tggtgtaaac tttataggaa acgttaggaa cggtagtaat tacatccatg
     2821 tcgaattctc tatcgagcct ttcctgaacg atttccatgt gcaacaatcc cagaaatcca
     2881 catctaaatc caaaacccag cgcggccgat gattcgggtt cgaacaccag tgaagcatcg
     2941 ttgagttgta gcttttcgag cgaaccgcgt aattcttcgt attcatcggc atctacagga
     3001 taaataccag caaaaaccat gggtttcacg ttgctgaatc cttcgatggg tttatcacaa
     3061 ggtcgttcca catgcgtgat agtatcgcct accttgactt cctttgctga ttttattcct
     3121 gaaatgatat aaccaacatt tccagcacta agctcgttgc atggtttacg ctccaaaccc
     3181 aatattccta tttcatcggc atgatattct ttacccgtgg caacgaattt tacgaaatcg
     3241 ttcgtgcgaa gggttccatt gaaaaccctg aaataagcaa tgattcctct aaaaggattg
     3301 aaaacagaat cgaatatcag agcctgcaaa ggggcttcgg gatcaccttg gggaggtgaa
     3361 attctttcaa cgattgcctt taggatttct tcgacctgat atccggtttt ggcactcact
     3421 gcaatgattt catcgggcga acatcccaaa aggtcaatca tctggtcgga aacctcatct
     3481 atcatggcgt tttccatatc tatcttattg agaaccggaa tgatggtcaa ttcgtgatcg
     3541 agggccagat aaagattgga aatagtttgt gcctgaatgc cctgcgtagc atctacaacc
     3601 agtagagctc cttcgcaggc tgctatgcta cgtgatacct catacgaaaa atcgacatgc
     3661 cctggcgtat ctatcagatt gagaatgtaa tcctcattat tgtaatgata attcatctga
     3721 atagcgtggc ttttaatggt tatgccgcgc tcacgctcca gctccatatt gtcgagcact
     3781 tgctgctgtt gctgacgttc gctgatggta tcagtaagtt ctaaaagcct gtcagccagc
     3841 gtgctcttgc catgatcgat gtgggcaatg atacaaaaat tgcgaatgtt tttcatctct
     3901 gcaaatttaa aagcaaaata acgattcgac tatatttcgg aaaatttcct tcatcgagat
     3961 gctcgatttc gatgtcattg cttatttctg gtcattataa aagtcgttga taaatgaatg
     4021 aattcagggt ttcattacaa ttaaatcgat tttcttcatt cataatctga caaactacag
     4081 gataaatctc agaaaattaa taagaagagt tctttatgga aacttttagg aaaacttcat
     4141 cccatgaggt tcattatctt tgcttgtgaa agaggagaga attcaaaatg agaaagattg
     4201 ctcttgtttt cgactttgat gatacattgg caccagacag tacctcggct tttttggaat
     4261 ggatgggagt gaatgcgcaa gacttctgga aaaatcgcgt agatcctctg tgcagtgatg
     4321 gttgggatcc cgttccggcc tatctttaca gccttttaaa gctttcgaac gaaaatgagg
     4381 aatacaaaat tacccgccaa tgtctcgagg aatttggttc gaaaatgaaa ttccataagg
     4441 gcgtgatgaa aattttctct tttcttatta gctattttgc caaacattat cctgaaatct
     4501 ctctcgaatt ttatattatt agttcaggaa ttggcgatat tattcgtaac tgtcgcatcc
     4561 ggaaattttt cagcgatatc tggacctgtg attttcacta taatgaagaa ggatgggcta
     4621 tttttcccaa aaacatcgtt tcgtttaccg acaagacaag atatttattt cagatttcga
     4681 agggcataag ccgcgaggat tccaaagccg accattatgc ggtaaatcgc agggtatcgt
     4741 cgaatgggta tcgtattcca ttgcagaata tgattttcgt tggagatggc ttgacagatg
     4801 ttccctgttt ttcggtgatt aggcgacatg gcggtattgc tattggggta tacgacaaac
     4861 atcgacatga acgctgggca aaagcatgga attttttgga agaagaccgg atagacaatc
     4921 tcctttcggc cgattattct ttggattcag atttgactcg ctcactgatt atggccatcg
     4981 aacgcattgc cgacagaatg taaagtttgg agtgaagcca tagaaatagg tatggctgaa
     5041 tcttcatcct cgcccaaaat atctccacag gctttaactg aaaaagaaat tctgtactaa
     5101 caaattgaat ttcgatgaaa tttgagataa taattttaga tcgtttctac gggacttgaa
     5161 tttgtttttc ttattttttg tctaccgata tattatcttt acaggacaaa taaatctatt
     5221 catcttccat tcgttagttt tgcaaaaatt ttaaaggttg ttatagtcta attatgaact
     5281 cgatatgaat aaaaatgcaa ttccttattc tatgaaagtg ctgtaaagtt cgaaataatg
     5341 aaagaacttg gattcttgat tgaagagatg atttatcgtc aggagaggtt atcggaagaa
     5401 taatttcact ggtaaagctc ttaaagataa ttcgattcgg gcaaagatta aagaggacga
     5461 tgaatttgag aatatttctt aagaaattct ctgaaaaaac ttcaaaatgc ttgcaagatt
     5521 gaaaatttct tttacttttg cttcctaaag attgtgattc atattaaatg ccagagtcaa
     5581 caataatatt atcagtttta gttgtattaa tggttttctt cttgaaagag acattaagca
     5641 taactcttac gaaacaggaa acttccctat tggttaaccc atcatctatg cttccgctat
     5701 ccgaattttt tgttaattta ttttatatca ataatttaac tatcatcccc cccccccccn
     5761 nnnnnnnnnt aaatagtaat gcaatactta gtcttgtcat tttctttttt ggagtttttg
     5821 ttataatacg aatacctgat aatctattgg agaatatgat gaatggatct agtgataaaa
     5881 aatttaaaat catccccccc cccccccatc ctcactgcaa aatcacgaat ttattcattt
     5941 cctaaatcat agaggtagag actgggtaat tttggttttt taacattaac aaactcttac
     6001 caaaaacaat taataaacaa attttaatta ataatttaaa aaactaataa aaatgaaaag
     6061 tttaaaaata aatttatttt tagcattacc tatcatgata attttaggta catcatgttc
     6121 aaaagaacaa gtaaatcaac aaaaaaatca gcttaaaacc tctgatacat gtctgattaa
     6181 aattgctaat attgattcta taggaatacc acatttagct attgactcat cccgtatttt
     6241 aaatgcctat caaacatata tgaataatta caatgatgat tcgcttaata taaagtatta
     6301 cgcggattcc ttaaacttca actggtatta taacgatagt acagacgttg aatcattggc
     6361 aaataggcaa ttttatctta tcatttacgg ccataaattt caaaatgatg aattttatgg
     6421 gtattcaaaa atagccactg aacttataag aatcgaaaat gaagtatttt ttaatagatg
     6481 caatgacaat aaatctattg gagttactta ctcatatgaa tgcataaata aaggctgtgc
     6541 tgctggatgc gaatgggtat atgcctggca ttggtggatt tttcatggaa gaattacaaa
     6601 ttgcacatgc ccagaaactc ctggtgaatg tgaatttcac atgggggtta aacatgattt
     6661 atcgcttttc taattattaa ttcaaatcaa tttttctgcc tacaaatgcc tatatctatt
     6721 aatctagcta atttaagaat ttaacattaa tagaagtagg catttttaca agataattta
     6781 aattatttat tctgttattt ttttaattta ttattttaca ttatgaaaaa gaattttttt
     6841 attcccatta ttacttcgct tattttatta tttcttagtc caactcttca ttcacaaaat
     6901 cctattgtta ttgctgaaaa acaaggcagt aactataaga ttatttatga ttctgtaaaa
     6961 ttaatccaat tgattatcga agaccttgaa tttttttata aggatgagca tgttttaata
     7021 actccctcaa aaatatatca gcttcctttt tgggaacctt ttgatagctt gaaaactttt
     7081 ctcaaagcac gctttaatga aagtagtcat ataattggtg gttataccaa agatttgact
     7141 tataaaggat cttttgatca tcctgaactt attggtgaag aaatacttgg tctcatctta
     7201 aaagctaata acgagcccaa agatacaagc ggatttaaag tttacgccct aaaaagcaaa
     7261 gtcgatagta ttaaaatcga gaaaattcat ggtcagtggt gtttagtagg ctatggaaaa
     7321 tatgattcgg ccgatgctaa agcttattat tttttgaaga aaaaaggcaa aaaaatgatt
     7381 ttatccgtaa aggataatat attagaaggg atggtaatat gttcggtggt taaccctgaa
     7441 tatgattgta atccagttct tggagagtat ggttgtgaat gtatggaaga taaagggaaa
     7501 caaaagggtt gcgatccgcg aatcgaccag cgcagtactt cgctgtctgg tcatagttct
     7561 tttggtatga gttcactctt accctcgccc tatcctttct aaaaagtcca actatttctt
     7621 tgaacaaatt ttttaattat taaatattct gttaaatcaa aaaaaatcat tatgaaacaa
     7681 gtttttttag cacatactca ggagaaagtt gcgattatcg tcctggcagc agtcctattt
     7741 tctatgcttt cgtgtaacaa aagtagcaag caggttgcta ttgcgagatt gaattcgaag
     7801 gatagcagtt tcgagttttc gatggatacc aatcggctga aagccttgat gattgaaggt
     7861 ttcgatattg atcgctccga ttctaatatt ctattaaccc ccaccgccat ctattatgcc
     7921 aatattatgg taagtgatac gcatacagct cctgtcatgg cttatttcaa ggaaagactt
     7981 cccaagggct ctcaggcaac cattactttt acaactaccg aaaataagag cgatacgggc
     8041 agtatattca agaccagagt cattttagct gctgatgccg aacccataca tttcgatggc
     8101 ttaacgaaga tggaagcaaa aacccaaatt aatggcatca aaatttcgaa aattaggagt
     8161 gcttattggc ttgaagaaag ggtatttatg accaactgtc ggtggcactt ttctttcgtt
     8221 taaagaaatc tgg
//
LOCUS       DAOORU010000108         8163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_10858_length_8163_cov_6.055131, whole genome shotgun sequence.
ACCESSION   DAOORU010000108 DAOORU010000000
VERSION     DAOORU010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10858_length_8163_cov_6.055131"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="PLF20_07395"
     CDS             complement(<1..433)
                     /locus_tag="PLF20_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80859.1"
                     /translation="MTQEINYTFDFSYDHDWRLTGTTFTMNGISKRLITLEYNELSQL
                     KAKYFNGTATAYLQRQLYHYNARGWLTSINNPYECPNDVFAMKLYYGNVPTYANSLAT
                     SCYNGNIAMIEWFTQNQNTYVNGYGFRYDGLNRLKAGRFFSR"
     gene            complement(932..3343)
                     /locus_tag="PLF20_07400"
     CDS             complement(932..3343)
                     /locus_tag="PLF20_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX80860.1"
                     /translation="MKKLSLIILFFFNIAWLWGQKIQVIDRSTLQPIAGASVFVVHDG
                     QKIGVVTNQRGMADISGIISSNLPVHITAIGYKEQAVSVEALEHTDYKVMLIERPYDL
                     NEIVVSASRFEEKRANVPQQMMMIKQKDIKFLSQPTSAELLQSSGQVLVQKSQLGGGS
                     PILRGFEANKVLLVVDGIRMNNAIYRGGHLQNIISIDNSMLEKVEVIYGPGSVVYGSD
                     ALGGVIHFHTLKPILAENGKTLTKTHAFTRYSTAYEEKTAHVDFNIGTRRFASLSSIS
                     VSDFGDLRQGDVRNPFYGDWGKRLFYVERIAGKDSIMTNENPNIQKGSAYTQYDFMQK
                     FLFKQSEKVSHTLNLQFSTSSDINRYDRLTEVSGGKPKYAEWYYGPQKRLLAAYQLEL
                     GKAQIWDRAKLIAGYQNIEESRHNRSFGKSKLSHRTEKLDVFTLNLDFDKQIAKQEIR
                     YGLEGTYNDVNSEAFAENINTGEISRLDTRYPDGGSKIRTLAAYVTHTWEINPKFILS
                     DGLRYSNIYLNAKFNDTTFFPFPFNEVTQKSSSLNGNLGLTYMPGKDWRFTLLGSTGF
                     RAPNVDDLAKVFESVPGSVIVPNPDLKPEYIWNAELGISKLFMNCVNVELTGYYTLLK
                     DLLTVEPSLFNGQDSILYDGVLSRVTTSVNADEGYIYGLSGTLIAQVTDNLIISSTLH
                     YTYGRIKTDTVDYPLDHIPPIYGKTSFRLNLNKFKGEFYVMYNGWKRLEDYNVKGEDN
                     LNYATEKGMPAWYTLNLRTSWQIDPRFEIQLALENILDQNYRVFASGISAPGRNFSVT
                     LRGNF"
     gene            complement(3463..3972)
                     /locus_tag="PLF20_07405"
     CDS             complement(3463..3972)
                     /locus_tag="PLF20_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80861.1"
                     /translation="MRKIWLLLVVSIFLFYTATYYPLFFLRRAEIKHEIKKIMLATNQ
                     FDTLSFSPSAFHSIRWIEKGKEFIKEGKMYDVANTIQKNDSIIQITVIEDKEEDNLYR
                     TLRNNTQNSDANSFYQIIKLLTGTIFIIQNQNFFIFIPAGEIEFLLSNIKPSKTFEEV
                     PSPPPRCII"
     gene            complement(4239..4376)
                     /locus_tag="PLF20_07410"
     CDS             complement(4239..4376)
                     /locus_tag="PLF20_07410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80862.1"
                     /translation="MLEGIIQKTWIPKGINKGIILEAETFSKEAPTEENGKENKREDI
                     V"
     gene            complement(4706..4779)
                     /locus_tag="PLF20_07415"
     tRNA            complement(4706..4779)
                     /locus_tag="PLF20_07415"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4743..4745),aa:Asn,seq:gtt)
     gene            4867..5859
                     /locus_tag="PLF20_07420"
     CDS             4867..5859
                     /locus_tag="PLF20_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOX80863.1"
                     /translation="MKHILGIGNALVDMITSIPGDDVLHHLSLPKGSMTLVDAALAQN
                     ITKTTSKFSPKLTSGGSAANTIFGLAHLGLQVGYLGKTGKDELGEIYENDLKACGITT
                     HISKSTTPTGRAVALISPDSERTFAVYLGAALELSPYDIMESILAQYAHVHIEGYQVQ
                     NHEVLLKILERAQACGCSISLDLASYNVVEENREFLQYILNNYVNIVFANEEEARALT
                     GEEPIGALDKLSQICPMAIVKIGKNGSMIKTNEKVWKIDAIEANAIDSTGAGDMYASG
                     FLYGLVHHYDIGTSGKIASLVAGKVTEIEGARLPGLVWDEIRQQIQWFIKNSVR"
     gene            5958..8087
                     /locus_tag="PLF20_07425"
     CDS             5958..8087
                     /locus_tag="PLF20_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOX80864.1"
                     /translation="MKVYQTKEIRNIAIVGAAKTGKTTLAEDMLYEGGVINRRGSIED
                     KNTVSDYRDIELERQNSIYSTVLYAELNDKKINMIDCPGFDDFVGEVLAALKVTDTAL
                     MLINAQNGVEVGAEINFRNASKAKMPLIFVINHLEHENSNFDETIRQMRQSFGGGVMI
                     CQYPVNEGLGFDAVIDLLQMKMLKFNASGNTEILEIPASEKVKADEMYSAMIEELAAN
                     EESLMEKFFENGSLTEEEISEGLKIGLINRGIFPVFCIHAKQNLGVRRLMEFIISSVP
                     SPDEAPRAKTIDGNEVTYNPADPACAFIFKTTMEPHLGEMSFFKVYTGEITEAMDMIN
                     AENGSKERMSQLFAVAGRNRQKIEKVMPGDIAATIKLKNVRTNHTLNSPKAPDVKIEP
                     IVFPAPKISFAVKAKNSTDDEKVILILNDLAKIDPTLVVEQSKELRQLIVSGQGELHL
                     NIQKWLIENIYKIPIDFLPPRIPYRETITKPAKSTYRHKKQSGGAGQFGEVHMMIEPY
                     YDGMSNQSEFPIRTQEVYDLDWGGKLVFNNCIVGGAIDARFMPAILKGIMEKMEQGPL
                     TGSYARDIVVNIYDGKMHPVDSNEISFKLAGRNAFKEAFKNAGPKILEPIYEVEVILP
                     EDKFGDVMTDLQGRRAIILGMDSEGNYKKIIARVPLAEMNRYSTALSSLTSGRATYSL
                     KFVEYAQVPPDVQTALLKTYEEQETEE"
BASE COUNT         2426 a         1610 c         1721 g         2406 t
ORIGIN      
        1 atctggaaaa aaatctgcct gctttcagac ggttgaggcc atcataccta aaaccatatc
       61 cattcacata agtgttctgg ttttgtgtaa accattctat catggctata ttgccattat
      121 aacagcttgt agccaaacta ttggcataag tgggcacatt gccgtagtaa agtttcatgg
      181 caaatacatc gttggggcat tcgtaaggat tatttatgga agtaagccag ccacgagcat
      241 tgtaatggta taattgccgc tgcaaataag ccgtagccgt accattgaaa tattttgcct
      301 tgagttgcga taactcatta tattccaatg taatcaaacg tttcgatatt ccgttcatgg
      361 taaaagttgt acccgtaaga cgccagtcat gatcgtatga aaagtcaaaa gtataattaa
      421 tttcttgagt caaactatat ttatgagaat tttttgattt tatgagagcc ccgttgaaat
      481 aatactggtt accggtatat gtatcattgt tgtaaatctg cacggggcga ttgtaagcgt
      541 cgtaaaaagt aacttcgtaa agtaaaggaa caaataattt gccagtgagt tggccttgag
      601 gattttttat tgcagaatca ctaaactgag gctcagcctt ccaatattgc aggtactcat
      661 tctcggcgtt accatcacag ttaaagtcgt aattatcatt gaaatataca atttctacct
      721 taacaggata ggaatcactg gctattattt ttgcaaactc aattggccaa gacagcttga
      781 attgttcaac tgtagagtca aataaatggt tcttcctttt tagtccagga taatcccaaa
      841 cgcaaataaa gctgtcccaa tccgttttga ataatctcga ttcaaggaat gggacagcta
      901 tgaaaaaaat tactctgttc gaggagtatt tttagaaatt ccctctgagg gttacggaga
      961 agtttctgcc tggtgcgctg attcccgaag caaatactct ataattctgg tcgagtatat
     1021 tttcgagcgc cagttgaatc tcaaatctgg gatcgatttg ccatgaagtg cgcagattaa
     1081 gggtgtacca ggctggcatg cctttttcag tagcataatt gaggttgtct tcacccttca
     1141 cgttgtagtc ctccaagcgt ttccatccgt tgtacatcac ataaaattcg cctttgaact
     1201 tattaaggtt taagcgaaaa ctcgttttgc cataaatggg aggaatatgg tcgagaggat
     1261 aatccacggt atctgttttt atacggccat aggtatagtg cagggtggag ctgatgatta
     1321 aattatcggt cacctgagca attagggtgc cactcaatcc ataaatatag ccctcgtcgg
     1381 catttacgct ggtagtaacc cggctcaaca caccatcata aagaatgcta tcctgtccat
     1441 tgaatagaga aggctcaaca gtaaggaggt ctttcagcag ggtgtaataa ccagtaagtt
     1501 ccacattgac gcaattcata aagagtttgg agatacccag ttcggcattc cagatatatt
     1561 cgggtttcaa atcgggattg ggcactatta ccgagcctgg taccgattcg aataccttgg
     1621 ccagatcatc cacattggga gcacgaaagc ccgtagaacc caacaacgta aatctccaat
     1681 cctttccagg catataggta agtcccaaat ttccattaag tgagctggat ttttgagtta
     1741 cttcgttaaa aggaaagggg aaaaaggtcg tatcgttgaa tttggcattc aaataaatat
     1801 tgctataacg cagtccatcg gagaggatga atttagggtt gatttcccat gtatgagtaa
     1861 cataggcagc cagtgttcgg atttttgaac ccccgtcggg atagcgagta tctaatcgac
     1921 tgatttcgcc tgtattgatg ttttcggcaa aggcttcaga gttcacatcg ttataggttc
     1981 cttcgagccc gtaacggatt tcctgctttg ctatctgttt gtcgaagtcg aggtttaaag
     2041 taaaaacatc gagtttttcg gttctatggc tcagcttgct tttcccaaat gagcgattat
     2101 gacggctttc ttcaatattc tggtatccgg caatcaattt ggccctgtcc caaatttgtg
     2161 ccttccccag ttcaagttga taagcggcaa gcagacgttt ttgaggccca taataccatt
     2221 cggcatattt tggtttgcca cccgaaacct cggtcaaacg gtcataacga ttgatgtcgg
     2281 atgatgtaga aaattgcagg tttaaggtgt gtgatacctt ttcggattgc ttgaagagga
     2341 acttctgcat gaaatcgtac tgggtataag cagaaccttt ctgaatgttg ggattttcgt
     2401 tggtcataat gctatctttc ccggcaatac gttccacgta aaaaagacgt ttgccccaat
     2461 ctccataaaa tggatttcta acgtcgccct gtcgcaagtc gccaaaatcg gaaacgctaa
     2521 tcgaactcag cgaagcaaat cttctggtac cgatattgaa atcgacatga gcagtttttt
     2581 cttcataagc ggtggaatat cgggtaaatg catgggtttt tgtaagagtt ttcccattct
     2641 ccgctaagat gggttttaga gtatggaaat ggatgacacc gccaagtgca tcactcccat
     2701 aaactactga gccagggcca taaatcactt ccactttttc gagcatagaa ttgtcgatgc
     2761 ttatgatatt ttgcaaatgg cctccacgat aaatagcatt attcatgcgg atgccatcta
     2821 ctaccaaaag aaccttgtta gcctcgaaac ccctcaatat gggactacca ccgcccaact
     2881 ggctcttttg caccaatact tgtccagaac tttgcaataa ttcggccgaa gtgggctgag
     2941 aaagaaattt aatatccttt tgcttgatca tcatcatctg ttggggaaca ttggctcttt
     3001 tttcctcgaa acggctggcc gaaaccacaa tctcgttcaa atcgtaaggc ctttcgatca
     3061 gcatcacttt ataatccgta tgctcgagtg cttcgacgct tacagcctgt tctttataac
     3121 ctattgccgt tatatgtaca ggaagattcg atgaaatgat tcctgaaata tcggccatgc
     3181 ctctctgatt ggtaaccaca ccgatctttt gtccatcgtg aacaacgaat accgaggctc
     3241 ctgcaattgg ttggagcgta cttctatcga tgacctgaat cttctgtccc caaagccagg
     3301 caatattgaa aaaaaacaag atgattaaag ataatttttt catattcaat aagttattta
     3361 atagtgaaag taaataaatt gtaatatcac tctttttttg tccttaatat gtgataagag
     3421 cataagagtg ataagaaaaa agataagatc gaggtatgag attcaaatga tacaacgtgg
     3481 aggtggagag ggcacttctt caaaagtctt cgaaggttta atattggaga gcaaaaattc
     3541 tatttctcct gctggaataa aaatgaagaa attttgattt tgaatgataa aaatagtgcc
     3601 cgtaagtaat ttaattattt gatagaatga attcgcatca gagttttggg tgttgtttcg
     3661 gagagttcgg tataggttat cttcttcttt atcttctata acagttatct ggatgatgct
     3721 gtcgtttttc tgaatagtat tggccacatc gtacatctta ccttctttga taaattcttt
     3781 tcctttttcg atccagcgga tagagtggaa ggcagaagga gaaaaagaca aggtatcgaa
     3841 ctggtttgta gcgagcatta tcttttttat ctcatgtttg atttcagcac ggcgaagaaa
     3901 aaacagagga tagtaagtag ccgtgtaaaa tagaaaaatg ctaacaacca atagcaacca
     3961 gattttgcgc attatagcgt gagtttctgg acaaaattac acgatttatc tttcgttcca
     4021 ttccctcaga tgatttggtt ggaaaatacg ctcaagtgtt tcgaatattg tcgaaacttt
     4081 tgaaatatgc ttctttgtaa acgaatcaga agaggtgatt ttacatctga aaaaaagggt
     4141 ataaaaacta ttttgcccct tatgtcaatg atagatcgaa atttttgctc ctgctgctgg
     4201 attccgagca aactcgggac gatcccctga ttattcattt atacaatatc ttctctttta
     4261 ttttctttgc cattctcttc tgttggggct tcttttgaaa atgtttccgc ttccagtatg
     4321 atacccttat ttattccctt tggtatccat gtcttttgga ttattccttc caacatctta
     4381 cgcgatttct atcgcttaag ccataactca ctttttattg cttctccctt gttttgaaaa
     4441 cctattactc ttttcatctt cgatagacgt ccacttcgag tactatataa ccccaccgaa
     4501 cagttatgct cccataatca acgtcccggt taactcgtac ttcctgtgta atttcttccc
     4561 gtcttctgag attgaagtat atatacgtag tacattacta ttaaaaataa tattagccgg
     4621 ctaataaacc ggctatcatc catgtaatat tttattctcc ttcttggctc ccgagcaggc
     4681 tcggaaggac cctctgaaaa ttctgctccc cctgctggac ttgaaccagc gaccctctga
     4741 ttaacagtca gatgctctaa ccagctgagc taaggaggaa ttcttatgaa agagggagca
     4801 aaattaataa aaatttctta ctttggctac atttgcaaaa attttatttg taaagaccac
     4861 agactcatga agcacatact tggcattggt aatgctttag tagatatgat taccagtata
     4921 ccgggcgatg atgttcttca tcatttgtcc ttgcccaaag gtagtatgac cttggtggat
     4981 gctgcattag cccaaaatat tactaaaacc acttctaaat tctcacccaa gttaaccagt
     5041 ggaggttcag cggcaaatac catttttggt ttggctcatc ttggactaca agtgggttat
     5101 cttggtaaaa caggaaaaga tgagttaggc gaaatttacg aaaacgatct gaaagcctgc
     5161 ggaatcacga cgcatatttc aaaaagtacg acacccacgg gcagggcagt agcgcttatc
     5221 agtcccgatt ctgaacgtac ttttgcagtt tatttggggg ctgctcttga gttgtcgcct
     5281 tacgatatta tggaaagcat tctggcacaa tatgctcatg ttcatattga aggttatcag
     5341 gtacagaatc acgaagtatt gttaaaaata ttggaaaggg cgcaggcttg cggttgcagt
     5401 attagcttgg atttggccag ttacaatgta gtagaagaaa atcgagaatt tttgcagtat
     5461 attttgaata attacgttaa tattgttttt gccaatgagg aagaggcaag agccttaacg
     5521 ggagaagaac ctattggagc acttgacaaa ctttcgcaga tatgcccaat ggccattgta
     5581 aaaattggga agaatggttc gatgattaaa accaatgaaa aagtttggaa aatcgatgca
     5641 atagaagcca atgccattga cagtacggga gctggagata tgtatgcctc tggatttctc
     5701 tatgggcttg tgcatcatta tgatatagga accagcggaa agattgcttc gttggtcgca
     5761 gggaaagtaa ccgaaatcga aggtgccagg ttgcccggtt tagtctggga tgagattaga
     5821 cagcaaatcc aatggtttat taaaaattcg gttagatagc gaaaaatttt tagtgggcgc
     5881 aaacatttgg caatcgaagg cttttttata attttgccgc tcaattgcaa atacttaaat
     5941 tataaccatt taaacaaatg aaggtttatc aaacgaaaga aatcagaaat attgccattg
     6001 taggtgcggc gaaaacggga aaaaccacac ttgcagaaga tatgctatat gaaggtggag
     6061 tcatcaatcg taggggttct atcgaagata agaatacggt ttccgattat cgcgacatag
     6121 agctggagcg tcaaaactcg atatattcca ccgttttata tgccgagctg aatgacaaga
     6181 aaatcaatat gatcgattgc ccgggttttg atgattttgt aggagaggta ttggctgccc
     6241 tcaaagttac cgatacggcg ctcatgctca tcaatgctca aaatggcgtt gaagttggag
     6301 ccgagatcaa tttccgcaat gcttccaaag ccaagatgcc tttgattttt gtgattaatc
     6361 atctggaaca tgaaaattct aacttcgatg aaaccattcg tcaaatgcgg caaagctttg
     6421 gaggtggagt aatgatttgt caatatcctg taaacgaagg acttggtttc gacgccgtga
     6481 tcgacctttt gcagatgaag atgctgaaat tcaatgcttc aggaaatacg gaaattttag
     6541 aaatacccgc ttctgaaaag gtgaaagccg atgaaatgta ctcggccatg atagaagaac
     6601 ttgccgcaaa cgaagaatca ctgatggaga agtttttcga aaatggctct ttaactgaag
     6661 aagaaatcag tgaaggtttg aagataggtt tgatcaatcg tggaatcttc ccggtttttt
     6721 gtattcatgc aaagcaaaat ttgggggttc gtcgtttgat ggagtttatt ataagttctg
     6781 tgccttctcc ggatgaagca ccacgagcca aaactattga tgggaatgaa gttacttaca
     6841 atccagccga tccagcttgt gcttttatat tcaaaaccac catggaaccc catctgggcg
     6901 aaatgagctt cttcaaggta tataccggtg agatcaccga agccatggat atgataaatg
     6961 ccgagaatgg ttcgaaggag cggatgtctc agctttttgc cgtggctggc cgaaatcgtc
     7021 agaaaatcga aaaagtgatg ccaggggata ttgccgctac cataaagctg aagaatgtac
     7081 gtaccaacca taccttgaat tcgcccaaag ctcccgatgt caaaattgaa cccattgttt
     7141 ttccggcacc caaaatctcg tttgcggtaa aggctaaaaa tagcactgat gacgaaaaag
     7201 tgatactgat tctcaatgat ctggcaaaaa tcgatcccac gctcgttgtc gagcaatcga
     7261 aagaactcag acagcttatc gtgagtggac agggagaact ccatcttaac attcaaaagt
     7321 ggctcatcga aaacatttat aagattccaa tagatttctt gccgccacga ataccttatc
     7381 gagaaaccat aaccaagccc gccaaatcca catatcgtca taagaaacaa tcgggaggtg
     7441 caggtcagtt tggtgaagta cacatgatga tagaacccta ctatgacggc atgtccaatc
     7501 agtcagagtt tccgatacgc acacaagaag tgtacgatct ggattggggt gggaaattgg
     7561 tattcaacaa ctgcattgtg ggaggtgcta tcgatgcaag gttcatgccg gcaattctca
     7621 agggaatcat ggaaaaaatg gaacaaggtc ctctcacagg atcatacgct cgcgacattg
     7681 tcgtcaatat ttacgatggt aagatgcacc cggtcgattc caatgaaatt tccttcaaat
     7741 tggcaggccg taacgctttc aaggaagcat tcaagaatgc cggacccaaa atcttggagc
     7801 ctatctatga agtagaagtc attcttccag aagataaatt tggtgatgta atgaccgacc
     7861 tgcagggacg aagggctatt atccttggaa tggacagcga aggaaactat aaaaagatta
     7921 ttgcccgtgt tccattggca gaaatgaatc gttactccac tgccctcagt tcacttacct
     7981 caggtagagc tacatattcc ttgaaatttg tcgaatatgc ccaagttccg cccgacgtgc
     8041 agacggctct ccttaaaact tacgaagaac aggaaacgga agaataaaat caatccgaaa
     8101 cccttgtttt cctgttttcc gcattgggac accctaaaaa tttttacaaa caattcggta
     8161 cag
//
LOCUS       DAOORU010000109         8093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_10990_length_8093_cov_5.534337, whole genome shotgun sequence.
ACCESSION   DAOORU010000109 DAOORU010000000
VERSION     DAOORU010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8093
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10990_length_8093_cov_5.534337"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="PLF20_07430"
     CDS             <1..1143
                     /locus_tag="PLF20_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80865.1"
                     /translation="FVCDGYQGTGYYHFNFGWGGSFNGYYYTNNLTPGGSNFNLAQEI
                     IINCHPDTVDYIYPNPSNEPENLTFRMGSIDDGNEPRYPHPVMSKQWLISPQNQIDSI
                     SSITINFHRFDLDPGQAFLYIYDGPTSNDSLLGSFSGNVLPPSLTSSSNQVYIEFLSP
                     PQSSHDGFFATYSSTQPIWCSGTTTYTEPYGTISDGSGKFWYRNRSICLYKIIPPNAQ
                     QVAIYFTKFLTEADHDKVKIFDYSSGQLLAEYSGHYDPNSPPPPVLSPSGKMYITFNS
                     NNTIRDDGWEGYYESYFVGQKDELEHHVRIFPNPAQNIIWIELLAELHPTRLTILNAI
                     GQIVSELILTNNEQPIEISVKNLQNGFYILNIQCDDQQFTGKILKY"
     gene            complement(1184..2299)
                     /gene="recF"
                     /locus_tag="PLF20_07435"
     CDS             complement(1184..2299)
                     /gene="recF"
                     /locus_tag="PLF20_07435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOX80866.1"
                     /translation="MWLRHLNLIQFKNFGELEVDFSPKINCFIGENGAGKTNILDAIY
                     YLSFTRSCFNSMDVQNILHGEEFFAIHGDYVMDSSLENKVHCIQKKGHAKEFSLDEKR
                     YERMADHIGLIPLVMLSPYDNDLIYDGSEARRKYIDGVISQFDHPYLDDLLIYNKALA
                     NRNLLLRQFADSGRFDPEMLEIYNPQLIGPGNRIFEKRQKFIDQFVPIFEKYYNLISA
                     EKEKISLHYVSQLAEKPMQTLLCENFDRDRLTRYTNGGIHKDDLEFIIENYPIKRFGS
                     QGQQKSFIVAIKLAQFEFTRELTGIKPILLFDDIFDKLDECRVETIVRLVGSENFGQV
                     FITDTQANRVYNIFIENPIHHKIFMVRNGELEEVDLI"
     gene            complement(2319..3203)
                     /locus_tag="PLF20_07440"
     CDS             complement(2319..3203)
                     /locus_tag="PLF20_07440"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF002187.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOX80867.1"
                     /translation="MKVNIDPNSGFCFGVIKAVETAEEHLQRGHELLGLGEMVHNEAE
                     LERLENLGLKTVSIEQFPDLKGKAVLLRAHGEPPSTYRIAAENSVQLIDATCPIVKRL
                     QEEMKKAWQSGKDQGVQMIIFGKPLHAEAIGLNGQVDNNGIIVENLDDLQTIDARRPA
                     HLFSQTTMDAGKFEKIAEKLREMYTETGQNLIIHKSFCRQVSNRMKSIKEFAASVDVL
                     FFVASKNSSNGKVLYQTAQSINERTFFISGIQDIDFQKIGIDWGKVETIGISGATSTP
                     PWLMHEIARLIENAFDLT"
     gene            complement(3847..4926)
                     /gene="mltG"
                     /locus_tag="PLF20_07445"
     CDS             complement(3847..4926)
                     /gene="mltG"
                     /locus_tag="PLF20_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOX80868.1"
                     /translation="MAYYVKNKYSSRRRRTSGQKIIFGVLIVCIVLALIIAYGLYSAL
                     FKPNVWTKGENIVYINIPTGSNFEDLKNMMYERGLIVNRKTFEWLARQRKLDKHVIPG
                     RYPLTNQMSNLELIRMLSGGHQVPVQLVFNNMRSKEQLASRISQQIEADSATLIRLMN
                     DNEFLRKYNLNSDNVFVLFIPNTYEFWWNTSAESFMDRMYREHKIFWDSTRFEKARNL
                     GLSITEVVTLASIIEQETNKNDEKSRMAGVYLNRLKLGWPLQADPTLKYVIGDWSIKR
                     VMGIHKEIDSPYNTYKYKGLPPGPICIPSIASIDAVLNAEKNNYLFFCAREDMSGYHY
                     FTASHEQHRKNAEKYRQALNKLGVY"
     gene            complement(4930..5460)
                     /locus_tag="PLF20_07450"
     CDS             complement(4930..5460)
                     /locus_tag="PLF20_07450"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOX80869.1"
                     /translation="MIQGDNIRLRALEMEDADWIFHFENDPSLWRVTQTVTPYSRYAI
                     EQYVLNASIQDIYGSRELRLVIETLRDKRPVGLIDLFEFDPLNRRAGVGVAIEQKERR
                     QGFASEALQLLIQHCFRVLALHQLYCNILVSNEASIQLFEKFGFEVTGRKKEWIIKHN
                     QWEDELFLQLFNPQTI"
     gene            complement(5463..6257)
                     /gene="dapF"
                     /locus_tag="PLF20_07455"
     CDS             complement(5463..6257)
                     /gene="dapF"
                     /locus_tag="PLF20_07455"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOX80870.1"
                     /translation="MTVNFAKYHGTGNDFILIDNRTDFFSVNKEFISNLCHRRFGIGA
                     DGLILLNKANGFDFEMLYFNSDGNQATMCGNGGRCIVAFAQKLGIIQQNAIFKAVDGL
                     HTARILEVDKHDTLIELSLNDVELYHFVGADCLLDTGSPHLVRFMEYSDLRKFNVKKE
                     GRKIRYRSPYTNEGINVNFVCETSEGIFVRTYERGVEAETLSCGTGVTASALAWSLNK
                     NLLRGPVEVFTRGGNLTVDFEKADEKFIHINLTGLATFVFTGKYSI"
     gene            6454..7896
                     /locus_tag="PLF20_07460"
     CDS             6454..7896
                     /locus_tag="PLF20_07460"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOX80871.1"
                     /translation="MKKYLSLLLVAIIGGMMAVGIYKAVEPKQSDLQNREIIPVQQVS
                     LTDAHAMPLPDFVEAANMSVHAVVHIKAEFARKNSLYEDFFENFFTPFSRPQPSQPII
                     GTGSGVIISPEGYIVTNNHVVQDAINLEITLNDKRVYKAKIVGTDPSTDLALIKIDEN
                     NLPYLVYGNSDDLRVGEWVLAVGNPFNLTSTVTAGIVSAKARNINILGDRTGQTIESF
                     IQTDAAVNRGNSGGALVNTRGELVGINAAIASGNGFYTGYSFAIPINLVRKVVKDLLQ
                     YGEVQRGYIGVQFQEVNAELAKKKKLDKIKGVYVSGVTENSSAEKAGIKPGDVIVAIN
                     NREVNSNSELLEIIGQYSPGDKIKLTIERNGEPKTYDITLTNEKGTTERIKHEEASFI
                     ENLGASFRPVPDEIKKQLGIQSGLEVTELRPGALSAIGIKKGFIITRIDRVRVNSVKD
                     IEDALKDKRGGILIEGIYPNGVRAYYGLGL"
BASE COUNT         2513 a         1712 c         1465 g         2403 t
ORIGIN      
        1 tttgtgtgtg atggttatca gggaactgga tattaccatt ttaattttgg ttggggaggc
       61 tcattcaatg gctactatta taccaataac cttacacccg gaggcagcaa cttcaattta
      121 gctcaggaaa tcatcatcaa ttgccatcct gatacagtcg attatatcta tcccaatcct
      181 tcgaacgagc ccgaaaatct taccttcagg atgggaagca tcgacgatgg gaatgagcca
      241 cgctatccac atccagtgat gtcgaagcaa tggctcattt ctccgcaaaa tcagatagat
      301 tctatttcga gcatcaccat caattttcat cgtttcgacc ttgaccctgg acaggccttt
      361 ctatatattt atgatggtcc cacttctaat gattctcttt taggttcgtt cagtggaaat
      421 gtacttccac cttccctcac ttcttcgtcc aatcaggttt atattgaatt tttgagccca
      481 cctcaatctt ctcatgatgg tttctttgca acttactcga gtactcagcc tatttggtgt
      541 agtggaacta ccacatatac cgaaccttac ggaaccatca gcgatggttc gggaaaattc
      601 tggtaccgaa accgaagcat ctgcctttat aaaattatcc cgcctaatgc tcaacaggtc
      661 gctatttatt ttacgaaatt cctcacggaa gccgatcatg acaaggtaaa aatcttcgat
      721 tattcctctg gccaattact ggcagaatat tccggacatt acgatcccaa ctcccctccc
      781 ccacccgttc tctcacccag cggaaaaatg tatatcacct tcaatagcaa taacaccata
      841 cgtgatgatg gttgggaggg ctattatgaa agctattttg tagggcaaaa agatgaattg
      901 gaacatcatg tgagaatttt ccctaatcct gctcaaaata taatctggat cgaactcctg
      961 gccgaattgc atcctacccg cttaaccata ttgaacgcta taggacaaat cgtttcggaa
     1021 ctcatactta ctaataacga gcaacctata gaaatttctg tcaaaaacct tcaaaatggt
     1081 ttctacatct taaatattca atgtgatgac caacaattta caggaaaaat cctgaagtat
     1141 tgaatataga aaaaccacaa aaagggaaat acactctaaa agttcaaatc aggtcaactt
     1201 cttccagctc tccatttcta accataaata tcttatggtg gatgggattc tcgatgaaaa
     1261 tattataaac tcgatttgcc tgtgtatcgg taatgaaaac ctggccaaag ttttcacttc
     1321 ctacaaggcg aactattgtt tcaaccctgc attcatcgag cttatcgaag atgtcatcaa
     1381 acagaagaat tggcttgatg ccggtcaatt ctctggtaaa ttcgaactgt gctagcttta
     1441 tggctacaat gaaagatttt tgttgaccct gtgaaccaaa tctcttgata ggataatttt
     1501 cgataatgaa ctccagatca tctttatgaa tacctccatt ggtataacga gtcaggcggt
     1561 cgcgatcgaa attttcgcaa agcagtgttt gcataggctt ttctgcaagt tgactcacat
     1621 agtgaaggct tattttttcc ttttcggctg atataaggtt gtaatacttt tcgaaaatcg
     1681 ggacaaattg atcgatgaat ttctggcgtt tttcgaaaat acgatttccc gggccaatca
     1741 attgaggatt gtatatttcg agcatttcgg gatcgaaacg gccagaatcg gcaaactgac
     1801 gaagcaataa gttacgattg gcgagcgctt tgttgtaaat gagcagatca tcgagataag
     1861 gatggtcgaa ttgggagatc actccatcga tgtatttacg acgagcctca cttccatcgt
     1921 aaatcaaatc attatcataa ggagaaagca tcaccagagg aatcaatcct atgtgatcgg
     1981 ccatccgttc gtaacgtttt tcatcgagcg agaactcctt ggcatgccct tttttctgaa
     2041 tacaatgtac tttattttca aggctactat ccatcacgta atctccatgt attgcaaaaa
     2101 attcttcacc atggaggata ttttgaacat ccatcgaatt gaaacatgaa cgagtaaacg
     2161 aaagataata aatagcatcg aggatattgg tctttccggc tccattttct cctataaaac
     2221 aattgatctt tggagaaaaa tcaacttcca gctcaccaaa attcttaaat tgaatcagat
     2281 ttaagtgcct cagccacatg ctgcaaaaat acgatacttt aagttaggtc gaaggcattc
     2341 tcgattaacc ttgcaatttc gtgcataagc catgggggtg tacttgtagc tccactaatg
     2401 cctatggtct ctacctttcc ccaatcaatt cctattttct gaaaatcaat gtcttgaatt
     2461 ccagaaataa aaaaagttcg ttcgttgata ctttgggcag tttgatagag caccttgcca
     2521 tttgaactat ttttactggc aacgaaaaac aaaacatcca cagaagctgc aaactcctta
     2581 atactcttca ttctgttact tacctgacgg caaaaacttt tatggataat aagattttga
     2641 cccgtttcgg tatacatttc cctcaatttc tcggcgattt tttcgaattt tcctgcatcc
     2701 atggtagttt ggctaaataa atgtgccgga cgtcgtgcat caatagtttg caaatcgtcg
     2761 agattttcca caataatccc attattatcg acttgaccat tcaatcctat ggcttccgca
     2821 tgtaaaggct tgccaaagat gatcatttgt actccttgat ctttcccact ttgccatgct
     2881 ttcttcattt cttcctgaag tcgcttaacg attggacaag tagcatctat gagctgaaca
     2941 ctattttcgg ccgcaattcg ataggtcgaa ggaggctcgc catgagcacg caaaagcact
     3001 gccttccctt ttaaatcagg aaactgttca atagaaaccg tctttaaacc caaattctct
     3061 aatcgctcta attcggcctc attatgcacc atttcaccca ggcctaataa ttcatgaccc
     3121 ctttgcaagt gttcctccgc cgtctcaacc gctttaatta ctccaaaaca aaatccactg
     3181 ttgggatcaa tgttcacttt cattagtcta tcgagtttcc ctattcattg ttctacccaa
     3241 gattcaatct gcaaactcaa tcatcttatc aaccagtacg agacttctta gttcgaacgt
     3301 cgaaaaaatt tcaatcataa aaatattcta caaagatacg aacacctccg aaggactcca
     3361 catcaattat gattttccac cccgagataa agaaaaagcc ttcgatatta ttgtgtcaat
     3421 acctaaatga tgtcgaccat tttttaagct agtcatgggg gatgcgagag aaaaattcac
     3481 tcatataatc gcagatattc acagaaaatc agctttaatc agcgaattaa atcattggca
     3541 ttgaaaatca gcgcgaggaa atgctggtgc ccattatctc taaaaagccg ataattaata
     3601 aataacgatt catctccaaa tttatattat aatagaaata ataatgtaat ttcctcataa
     3661 aaaataagga ggatttgagc aggttcagct atcaggttat atccaacctc ttgataaacc
     3721 aagttatatt tccaggcaga tgatagctat tctccttctc catacttcaa gttttaaact
     3781 caggtcgtgg tttcctctcg aagaaaatac tcgaaatcca aaaacgcttg ctgcaataac
     3841 aagaaatcag taaacaccca atttattcaa ggcctggcga tatttctccg cattttttcg
     3901 gtgttgttcg tgcgaagcag taaaatagtg gtatcccgac atatcctctc gtgcgcagaa
     3961 aaaaaggtaa ttgttctttt cggcattcaa cacagcatca attgaggcaa tagaaggaat
     4021 acagataggt cctggaggta aacctttata tttataggta ttgtaaggcg aatcgatttc
     4081 tttgtgaatt cccatcactc ttttgatgga ccaatcgcca atgacatatt tgagagtagg
     4141 atctgcttgg aggggccaac ctagtttcaa acggttgaga tatactccgg ccatacgaga
     4201 tttttcatcg tttttattgg tttcttgttc gataattgag gcaagagtaa ccacttcggt
     4261 gatgcttaat ccaaggttgc gggctttttc gaaccgtgta ctgtcccaga atatcttatg
     4321 ttcccgatac atcctatcca taaacgattc agccgaagta ttccaccaaa actcataagt
     4381 gttggggata aacaagacaa acacattatc gctgtttaaa ttatattttc tcaaaaactc
     4441 attgtcattc attaaacgta tgagagttgc cgagtcggct tcgatttgct gcgaaattcg
     4501 tgaagccaat tgttctttcg agcgcatatt gttaaataca agttgtacag gcacctgatg
     4561 tcctcccgag agcatgcgta taagttcgag attgctcatt tggttggtca aagggtagcg
     4621 cccgggaata acatgcttat cgagcttgcg ctggcgcgct aaccattcga aagttttcct
     4681 gttgacaatc aggcctctct catacatcat attcttcaaa tcttcaaaat tagagcccgt
     4741 aggaatattt atataaacta tgttttcgcc ctttgtccat acattcggct taaacagggc
     4801 agaataaaga ccataagcta tgatgagtgc taaaacaatg caaacaatca atactccaaa
     4861 aatgatcttt tgccctgaag ttcgcctccg cctcgaagaa tacttatttt ttacataata
     4921 tgccatatct taaatggttt gaggattgaa taattgcaag aaaagctcat cttcccattg
     4981 gttgtgtttg atgatccatt cttttttccg gccggtgact tcgaatccaa atttttcgaa
     5041 aagttggatg ctggcttcat tcgagacgag gatattgcag tatagctgat gaagcgctaa
     5101 aacccgaaag caatgctgga tcaatagctg aagtgcttcg ctagcgaagc cctgtcttcg
     5161 ttctttctgt tcgatggcaa cacccacacc agctctccgg ttcaagggat cgaactcgaa
     5221 aaggtctatc aaacctacgg ggcgcttatc gcgaagagtt tctatcacca agcggagttc
     5281 acgactacca taaatatcct gtatgcttgc attcaaaaca tattgttcga tggcatagcg
     5341 tgaataaggt gtaaccgttt gcgtaaccct ccataaggaa ggatcatttt cgaaatgaaa
     5401 aatccagtca gcatcttcca tttccaaagc cctaagacga atattatctc cctgtatcat
     5461 gattatatcg aatattttcc agtaaataca aaagtggcaa gccctgtaag attaatgtga
     5521 ataaattttt catctgcctt ttcaaaatcc actgtcaaat tacctcctct cgtaaaaacc
     5581 tctaccggac ctctgagcaa attcttatta agactccagg ctaaagccga ggccgtaaca
     5641 ccagttccac aacttaaagt ttctgcttct acgcctcttt cgtaagttct aacgaaaata
     5701 ccttccgaag tttcacatac aaaatttaca ttgattcctt cgttcgtata gggagatcga
     5761 taacggattt tcctgccctc ttttttcaca ttgaattttc gaagatcaga atattccata
     5821 aaacgtacca aatgtggcga acctgtatcg agtaaacaat cagccccaac gaaatgatat
     5881 aattccacat cattcaacga taactctatt agggtatcat gcttatctac ttctaatatc
     5941 ctcgcagtat gaagtccatc gactgcttta aaaattgcat tttgttgaat aatccccagc
     6001 ttttgagcaa aagccacgat acatcgccct ccattgccgc acatggtagc ttggttgcca
     6061 tcagaattga aataaagcat ttcgaaatca aatccattag ccttgttcag taaaatcagg
     6121 ccgtcagctc caatcccaaa acggcgatgg cataagttag aaatgaattc tttattcact
     6181 gaaaaaaagt cagttcggtt atctatcaat atgaaatcat tcccagttcc atgatatttg
     6241 gcaaaattta cagtcatttg ttcacatcaa ttaaaccaca ttaaacttat attaaaaagc
     6301 ctcgaattta aacagaaatg aaaatattgt caaaattacc gaagcgaaat tagtatgaaa
     6361 atgaatcaag gtataaaaat tgaatgaaat ttgtagaata aaaaaatagt taaaagtatg
     6421 aaagttatat ttaaaaattt aaaaatcaat gacatgaaaa aatatctttc cttgttgctt
     6481 gtagctatta taggtgggat gatggcggta ggtatttata aagctgttga acccaagcaa
     6541 tcggatctcc aaaatagaga aataattccg gtgcagcaag ttagccttac ggatgcgcat
     6601 gccatgcctc tgcccgattt tgtcgaagca gccaacatgt cggttcatgc cgtagtccat
     6661 attaaagcag aatttgcgag gaaaaactct ctttatgaag attttttcga aaactttttc
     6721 actcctttca gccgacctca accttcgcaa cctattattg ggacaggttc aggcgtaatc
     6781 atttcacctg aaggctatat tgttaccaat aaccacgttg tacaggatgc catcaattta
     6841 gaaattaccc tcaacgataa aagagtttac aaggctaaaa tcgtgggtac ggatccctcg
     6901 actgatttag ccttaataaa aattgacgaa aacaaccttc cttatctggt ttatggcaat
     6961 agtgatgatt taagggtggg ggaatgggtt ttggcagttg gaaatccatt taatctgacc
     7021 agtacagtta cggctgggat cgttagtgcc aaagcaagaa acatcaatat tttaggcgat
     7081 cgaacgggtc aaaccatcga atctttcata caaaccgatg ccgcagtcaa tcgtggcaac
     7141 agtggtggag cactcgtcaa tacacgaggt gaattggttg gtatcaatgc tgctattgca
     7201 tccggcaacg ggttttatac gggttactcc tttgctattc ctatcaatct tgttcgcaag
     7261 gttgtgaaag accttctgca atatggcgaa gtccagagag gttatattgg tgttcaattt
     7321 caggaagtga atgcagaatt ggctaagaag aaaaaactcg acaagatcaa gggagtctat
     7381 gtttctggtg taaccgaaaa tagctcggcc gaaaaggcag gtatcaaacc cggcgatgtt
     7441 atcgtggcta ttaacaacag agaggtcaat agcaattcgg aactgttgga aattattggt
     7501 caatatagtc ccggcgataa aatcaaactg accatcgaaa gaaatggtga acctaaaact
     7561 tacgacatta ccttgactaa tgaaaaagga actaccgaaa gaataaaaca tgaagaagct
     7621 tcattcattg aaaatcttgg tgcttccttt cgacctgttc ccgatgaaat aaagaaacaa
     7681 ttgggcatac aatcgggact cgaagtaact gaattacgtc ccggagctct gtctgccatt
     7741 ggaatcaaga aaggattcat tattacccga atcgataggg taagagtgaa ctcagtaaaa
     7801 gatatagagg atgccttgaa agacaaacgc ggaggcatac tcatcgaagg tatttatcca
     7861 aatggcgtaa gagcatacta tggactcgga ttataaataa atagattcac cttgcaatca
     7921 tataacaagg gtttcttgac ttatacagag aaaccctttg ccattgtatt ttacatcgta
     7981 aatctctttc agttcaaact gtaaaacatt tgatttatat caagtattcc atttgaaatc
     8041 taattttttc aaaaagggga actcgaaaca catctattca ctaaatataa gtc
//
LOCUS       DAOORU010000110         7973 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_11206_length_7973_cov_4.986108, whole genome shotgun sequence.
ACCESSION   DAOORU010000110 DAOORU010000000
VERSION     DAOORU010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7973
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11206_length_7973_cov_4.986108"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(26..304)
                     /locus_tag="PLF20_07465"
     CDS             complement(26..304)
                     /locus_tag="PLF20_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80872.1"
                     /translation="MKVLVHAELGGGVSEWKGVSGIDGMGVGEFVEWYTRNGGTLRHS
                     LLSGNYLPQGIKFIEITEPNGGLCKLCIPTIIDRVIQQAIAQHRLFVS"
     gene            complement(389..1972)
                     /locus_tag="PLF20_07470"
     CDS             complement(389..1972)
                     /locus_tag="PLF20_07470"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOX80873.1"
                     /translation="MRNKKKKILWALSLIFSIIVFPEITLGDDPIAIDRMITITRELP
                     PFHGVEAGGTFNVVINIGQLQEVKIEVDEDQIENVITEVVNEILYIKGNKLKNPVVNI
                     SITIPELKYLNASGAATFKLNGMNRVKEMKLMASGAANVELNVEIDHLLSDVSGAADV
                     KVNGRASWHKSDVSGAGSLKASELVSDTLIIFLRGAADAFVDVKDLAIVQKSGAANIK
                     YRTKPRILTDVGYSTQYKIDTADKGVVNLGGDSVVVDLGKLKIKVDDNEGVSTIKIGK
                     QKLIVDDDGNVKWKKETNQHCFTGHWGGFDLSLNGYLNSDGNMDFAGADRYLDLNMPK
                     SIGVHLNAYEQSIKISRSGNFGFITGLGLEWHNYRFDQGVYLDEKAYRLNGYLIDGVR
                     IKKNKLMVRYLTVPVLFEYQHKGNSKLELYHFTAGLLLGARIGSHTKTVYAEQLKEYQ
                     LIDPVNYEVWKTMTSPRQETEKVHDDFNLNPFKADVMVRMGWGYVNLFGTYSITTLFR
                     NHRGPELYPFSVGLTLIGW"
     gene            2119..2997
                     /gene="prmC"
                     /locus_tag="PLF20_07475"
     CDS             2119..2997
                     /gene="prmC"
                     /locus_tag="PLF20_07475"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOX80874.1"
                     /translation="MTLEISNNRVYHIYRHYRHKLSSIYPSEEASTIARMVMQEILGI
                     DQIKFLSEPDLRISESEILKIHFAFKDLLNHRPLQYVLGYAWFRNIRLKVGHGVLIPR
                     PETEELVGWILEIFLDKPNLRILDIGTGSGCIAISLAKELKGAQITATDISEMALEYA
                     RENASEHGTAIQFVLHDITSNAKSTQFEPFHVIVSNPPYVTQSEKSNMHPNVLRYEPT
                     EALFVNNEDPFIFYRSIAGYGQKFLDEDGWIYVEINERFPAMIQKIFSSHKYRNIETR
                     KDFNGKYRMMRAQKPE"
     gene            complement(3014..3271)
                     /locus_tag="PLF20_07480"
     CDS             complement(3014..3271)
                     /locus_tag="PLF20_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80875.1"
                     /translation="MKEIRILGILITDRIKEAGQTQKVLSEFAHIIKSRLGFHEVTEA
                     VCSRLGIIMLQLVGSIDEQDKLESALNQIGGIQVRRMQFEV"
     gene            complement(3377..4804)
                     /gene="hydG"
                     /locus_tag="PLF20_07485"
     CDS             complement(3377..4804)
                     /gene="hydG"
                     /locus_tag="PLF20_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOX80876.1"
                     /translation="MKFNPEKCRMKDERMKPFIDTDEIWDLLDHTKPDRVRVKETIAK
                     SLEKNRLTLEETALLINATETDLVEEIKQGARELKKRIYGNRIVLFAPLYIGNKCTNN
                     CQYCGFRTSNKAAVRKTLTDDEIVREVEALEDNGQKRLILVYGEHPDYDANYIAHTVR
                     LVCGVKKGKGEIRRVNINAAPLDIDGFRTVKQAGIGTYQIFQETYHPEAYKTYHLGGK
                     KTDFNWRLTALDRAQEAGIDDVGIGALIGLYDWRFEVMGLVRHANHLEACYNVGPHTI
                     SFPRIQNASSLDIDGRYLVNDADFAKLIAILRLAVPYTGMILTARENPRLRREVIPFG
                     ISQIDGGTRLELGTYSQNLNAEQDLNREQFKINDDRSLNEIIEELIQNDFIPSFCTAC
                     YRIGRTGEHFMEFSVPGFIKRFCTPNALLTLSEYLVDYAPQDTAQMGWKAIEQNLAHL
                     GDTANTQEIRRRIERIKQGERDLYF"
     gene            complement(4877..5506)
                     /locus_tag="PLF20_07490"
     CDS             complement(4877..5506)
                     /locus_tag="PLF20_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HOX80877.1"
                     /translation="MSHRSLPDKTIERLSQYRRNLLICLSQGKHHIFSHEIALIHHIT
                     PVQVRRDLMLIGYSGTLRKGYDVKELIDLIGLILDTEEGLKVAIVGIGNLGRSLIGYF
                     SGKRSKLNIVAGFDNNPDKIGKIYAGVHCYSSERMQEIIVEEEISIAIITVPADNAAI
                     VAERLVMAGIKGILNFSPKPLNVPPHVYLEEIDMITSLEKVAYYVKKQL"
     gene            complement(5524..6666)
                     /locus_tag="PLF20_07495"
     CDS             complement(5524..6666)
                     /locus_tag="PLF20_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HOX80878.1"
                     /translation="MAKLKILVVDDEPFIRSGIHRILDNFQVDYPFMTEPYEFEILDA
                     VTGEEAIEIINNHQPDIVLLDNKLPGIQGVEVLSYVKNKKLDIVVIMITSYASLDLAV
                     RATKDGAHDFVPKPFTPQELRSVIESTTKHIFLKRMTQQMNIAGKQIRFQFLSVLSHE
                     LKAPLNAIEGYLQMMKEKQLGDKIDDYAQVIDRSLARIKGMRTLILDLLDMTRIETGE
                     KEKKIERLNICAIAQTSIDTSKPMAIQRDVKIKLDCPKDLEFETDASDMEIIFNNLIS
                     NAVKYNKEGGSVTVRIHKDENNLVIEVSDTGIGMTEEETSRLFQEFVRIKNEKTRNIT
                     GSGLGLSIVKKIVDTYNGNISVQSTPDVGSTFIVTLPVQSLTDIIL"
     gene            complement(6681..7073)
                     /locus_tag="PLF20_07500"
     CDS             complement(6681..7073)
                     /locus_tag="PLF20_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOX80879.1"
                     /translation="MEAHKKTIFIVDDDPDYLFQLQVLLEKNGFRVITAESQREAQKI
                     IERTKPDLAIVDLMMENEDSGFILSYQIKRLYPDVPVIIFTAVTAETGISFETELGMN
                     SRWIKADLFLPKGLRPDQLIREINKLLK"
     gene            complement(7101..>7973)
                     /locus_tag="PLF20_07505"
     CDS             complement(7101..>7973)
                     /locus_tag="PLF20_07505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX80880.1"
                     /translation="QDYLNCGACGYDTCEEHAIAIAQGFAEPEMCLPYTIEKLHKSIN
                     DLNITNEKLAHAKAALKQSEKLAHMGQLSAGIAHELNNPLGVITMYANIMMEERPEND
                     PLRKDLELIVEQANRCKKIVSGLLNFARKNQVNLTETHLEQLVQRSLDSVVKPDNVKT
                     RMISEMKDPICFLDQEQMMQVLTNLEKNAIEAMPPEGGEITVTLKDNLHEVIIKVADT
                     GIGIEKENMDKLFTPFYTTKELGKGTGLGLPLIYGIVKMHKGQISVMSNNNKEEGPTG
                     TIFTITLPRNPNSV"
BASE COUNT         2217 a         1740 c         1451 g         2565 t
ORIGIN      
        1 atcagccagc ctcgtaatac aggattcagc tcacaaataa cctgtgctgg gctatcgctt
       61 gttggatgac tcggtcgatg atcgtgggaa tacataactt gcataaccct ccgttaggct
      121 cagtgatttc tatgaatttg ataccttgtg gcaaatagtt gccactcagt aaggagtgac
      181 gtaaggtgcc cccattacgg gtgtaccatt cgacaaattc tcctactccc atcccatcaa
      241 tcccgcttac acccttccat tcgctaacac ctcctcccaa ctcggcgtgt acaagaactt
      301 tcatcttgca agtagtaatg tatgcccgtc atacaaaaat ggctgccccc gttccggggg
      361 cagccataaa catccaatcc aacgcaaact accaaccaat caatgtcaat cccacagaaa
      421 atgggtaaag ctcgggacca cgatgattcc gaaacaatgt tgtaatactg tacgttccga
      481 acaaattgac ataaccccaa cccattctta ccattacatc ggccttgaat ggattgagat
      541 taaaatcatc atgaaccttt tcggtttctt gacggggaga tgtcatggtt ttccaaactt
      601 cgtaattaac gggatcgatc aactggtatt ctttcaactg ctcggcatac acggttttcg
      661 tatgtgaacc tatgcgagct ccaagtaaaa gcccagccgt aaaatgatat aattcgagct
      721 tcgaatttcc tttgtgctga tactcaaaca atacaggtac tgtaagatat cttaccatca
      781 gtttgttctt ctttattctt acaccatcta tcaaataacc attcaaacga taggctttct
      841 cgtcaagata aactccctga tcgaaacggt aattatgcca ttccaagcct aaacccgtta
      901 taaaaccaaa atttcccgaa cgagatattt ttatactttg ttcataggca tttaagtgaa
      961 ctccaatgga tttaggcata ttcaaatcaa gataacgatc agcaccggca aaatccatat
     1021 ttccatcact gtttaagtaa ccgttcaaac ttagatcgaa tcctccccaa tgccctgtaa
     1081 aacaatgttg gttcgtttct ttcttccatt tcacattgcc gtcatcatcg acgattaatt
     1141 tctgtttccc aattttaatc gtacttacgc cttcattatc atccacttta attttcaatt
     1201 tacccagatc gacaactacc gagtctcctc ccagattgac tacaccctta tctgcagtgt
     1261 cgattttgta ctgggtagaa taaccgacat ctgtcaaaat acgaggtttc gtcctatatt
     1321 tgatatttgc agccccgctt ttctgcacaa ttgccaaatc ttttacatcg acaaaggcat
     1381 cggctgctcc acgtaaaaat atgatcaaag tatcgcttac cagttccgaa gctttcaaac
     1441 ttccagctcc acttacatcc gacttgtgcc aactcgccct gccattcacc ttaacatcgg
     1501 cagctcccga tacatccgaa agcaaatgat ctatttcaac attcagctca acattggctg
     1561 caccactggc cataagtttc atttctttta cccgattcat cccatttaac ttgaatgtag
     1621 ctgcaccact tgcgtttaga tatttcaatt ctggtatggt gatagatata ttcacaacag
     1681 gattcttcag tttattccct ttaatgtata aaatttcatt caccacttcc gtaatcacat
     1741 tttctatttg atcttcatcg acttcgattt tcacttcctg taactgacca atgtttatta
     1801 ccacattgaa agtgcctccc gcctcaactc catgaaaagg gggcaattct cttgttatcg
     1861 tgatcatcct atcaattgct atgggatcat cacccagcgt gatttccgga aaaacaataa
     1921 tcgagaatat caggctaagt gcccaaagaa tcttcttctt tttgtttctc attgctataa
     1981 attttgaatt tcttggtttt tgaattttat ttatgacgaa tttttacttc agatgttaca
     2041 aatattcggt ttgtttattc attatacgaa ttcgaatatc tttcaattat agttctggaa
     2101 acttactaac tttgctgcat gacacttgaa ataagcaaca atcgggttta ccatatttac
     2161 cggcattatc gtcacaaact ttcttcgatt tatccttcgg aagaagcatc caccatagct
     2221 cgtatggtga tgcaggaaat tttaggaatt gaccaaataa aatttctttc cgaacccgat
     2281 ctgcgtattt ccgaatcaga aatcctaaaa attcatttcg ccttcaagga tttattgaat
     2341 catcgacccc tacaatatgt actaggttat gcatggttta ggaatatacg gctaaaagtc
     2401 ggccatggcg tgcttattcc aaggcctgaa accgaagaac tggttggttg gatactcgaa
     2461 atttttttgg acaaacccaa tttgagaatt cttgatatag gaacgggaag tggctgtata
     2521 gcaatttcat tggcaaaaga gttgaaaggt gcccagatta cggcaaccga catttcagaa
     2581 atggctctcg agtatgcccg cgagaatgct tcagaacatg gaacagccat tcagtttgtc
     2641 ctccatgata ttacttccaa tgcaaaatca acacaattcg aacctttcca tgtcattgtt
     2701 tcgaatcctc cttatgtaac tcagtcggag aaatcgaata tgcatcctaa tgttttacga
     2761 tatgaaccta ctgaggcctt atttgtaaat aacgaagacc ctttcatttt ttaccgcagc
     2821 attgctggat atggacaaaa attcctggat gaggatggct ggatttatgt agaaatcaac
     2881 gaaagatttc ccgctatgat acaaaaaata ttttcttcgc ataaatatcg taatatcgaa
     2941 acaagaaaag acttcaatgg aaaatacagg atgatgagag cccaaaaacc cgaatgagca
     3001 tcttctggta gatttatacc tcgaattgca tccgcctgac ttgaataccc ccaatttgat
     3061 tcaaagcact ttccagttta tcttgttcat ctatggaacc aacaagttgg agcataatga
     3121 tccccagcct cgaacagact gcttctgtaa cttcatggaa gcctaaacgc gatttgatta
     3181 tatgagcaaa ttcggataat actttctggg tctgccctgc ttcctttatg cggtcggtta
     3241 taagaattcc caggattctg atttctttca tctgtcaaag attttcaaat ttaatacaaa
     3301 atattcataa tgaaacactt atactaatct tggaaggctc attcgattcc tattctgtat
     3361 atagtatttt aaaattttaa aaatacaggt ctctttcgcc ctgtttgatt ctttcgattc
     3421 gtcggcgtat ttcctgagta ttagctgtat cgcccaaatg agcaagattt tgttctatag
     3481 ctttccagcc catttgtgca gtatcttgag gagcataatc cactaaatat tccgaaagcg
     3541 ttagcagcgc atttggtgta caaaatcttt taatgaatcc gggaacgctg aattccatga
     3601 aatgctcgcc cgttcgccct attcgatagc aagccgtaca gaatgagggg atgaagtcgt
     3661 tttgaatgag ttcttcgatg atttcattga gtgaacggtc gtcgttaatt ttgaattgtt
     3721 cacggttaag gtcttgttct gcattaagat tttgagaata ggtacccaat tccaagcgtg
     3781 ttccgccatc gatctgagaa atgccgaagg ggattacttc tctgcgtagc cttgggtttt
     3841 ctctggcggt taaaatcatt cctgtataag gaactgcaag gcgaagtatg gctatgagtt
     3901 tggcaaaatc agcatcattc accagataac gtccatcgat atcgagagaa gaagcatttt
     3961 gaattctagg aaagctgata gtatgtggtc ccacattgta acaagcctcc agatggtttg
     4021 catgtcttac caatcccatc acttcgaaac gccagtcgta taaccctatc agggcaccaa
     4081 ttcctacgtc atctatccct gcttcctgag ctctgtcgag ggcagtaaga cgccagttga
     4141 aatccgtttt cttacctcca agatgatatg tcttataggc ttcaggatga taggtttcct
     4201 gaaaaatctg ataagtgcca atgcctgctt gctttactgt acggaaacca tcgatatcga
     4261 gtggtgctgc attgatattc actcttctga tttcgccttt tccttttttt actccacaaa
     4321 ccaaacgaac cgtatgggca atataattcg catcgtaatc gggatgctcg ccataaacca
     4381 ggatgagcct cttttggccg ttatcctcga gggcttctac ttccctgaca atttcatcgt
     4441 cagtcagggt ttttctcaca gcagccttgt tgcttgttct gaacccacaa tattgacaat
     4501 tattggtgca tttgttgcct atgtataaag gagcgaataa aacgattcgg ttcccataga
     4561 ttcgtttttt taattcacgt gctccttgct taatttcttc gacaaggtcg gtttcggtcg
     4621 cattgatcaa cagcgcagtc tcttcaagtg tcagccgatt tttttcgagc gatttggcta
     4681 tggtttcttt cactcttacc cgatcaggtt tggtatgatc gagcaaatcc caaatctcat
     4741 ccgtgtcgat gaaaggtttc attctttcat ccttcattct gcatttttct ggattaaatt
     4801 tcattgacca attgaattta aaattctttt gcaaatttac gactaataag agccctatat
     4861 tattctatac agaaaattat agttgtttct taacgtaata agccactttt tctaaagaag
     4921 taatcatgtc aatttcttcg agatagacat gcggaggcac atttaatggt tttggggaga
     4981 aattaagaat tccctttatt ccggccataa cgagtcgttc ggctacgata gcggcattat
     5041 cagccggaac agtaataata gctatgctaa tttcttcctc aacgatgatc tcctgcattc
     5101 tttcgctgga ataacaatga acaccagcgt agattttccc gatcttatcg gggttattgt
     5161 cgaatccagc cacgatattg agttttgatc tcttaccact aaaatagcca atgagagaac
     5221 gcccaagatt cccaattcca actatggcaa cttttaatcc ttcttccgta tcgaggatca
     5281 aaccaatcag atctatcaat tcctttacat cataaccttt tcttaacgtg ccggaatagc
     5341 ctatgagcat aaggtcacga cgcacttgta ccggtgtaat atgatgaatc agagcaatct
     5401 catgtgaaaa aatatgatgc ttcccctgcg ataagcatat aagaagattt cgtcggtatt
     5461 ggctcaatct ttcaatggtt ttatcgggta aagacctgtg gctcatcttc atagataaat
     5521 gatttataga atgatatcgg ttaaggactg aacaggcagg gttacgatga aagtgctccc
     5581 cacgtcaggt gtactttgca cggatatatt tccattatat gtatcgacaa tcttctttac
     5641 aatagataat cccaaaccac tgcctgtgat atttcttgtc ttttcatttt ttatccttac
     5701 aaattcctga aacagacgag aggtttcttc ttcggtcata cctattccgg tatccgatac
     5761 ttcgatcact aaattatttt catccttatg tattctaaca gtaacacttc caccttcctt
     5821 attgtatttt accgcgtttg atatcaaatt attaaaaata atttccatgt cggaggcatc
     5881 ggtttcaaat tccaggtctt taggacaatc gagctttatc ttcacatcac gctgtatagc
     5941 cataggtttt gaagtatcaa tagaagtttg agctatagca caaatattga gtctttcgat
     6001 tttcttttct ttttcacccg tttcgatgcg cgtcatatcg agcaagtcta aaataagtgt
     6061 acgcatgcct ttaatacgtg ctaacgaacg gtcgattact tgagcgtagt cgtcaatttt
     6121 atcaccaagc tgtttctcct tcatcatttg cagataccct tctatggcat tcaaaggagc
     6181 ttttaattca tgactcaaga cggatagaaa ttggaaacgg atttgtttcc cggcaatatt
     6241 catttgctgg gtcatacgtt tgagaaaaat atgttttgtc gtagattcta tcaccgatct
     6301 caattcttga ggagtaaaag gctttggaac gaagtcgtgt gccccatctt tagttgctct
     6361 gacggccaga tcgagggagg cataggaagt aatcataatg actactatgt cgagtttttt
     6421 gttcttaaca tacgaaagca cttccacacc ctggataccg gggagtttat tatcgagcaa
     6481 gacaatatcg ggctgatggt tattgatgat ttcgatagct tcttcgccag taacagcgtc
     6541 gagtatctcg aattcataag gttcggtcat aaagggataa tccacttgga aattatcgag
     6601 tatgcgatgt atgcctgagc gaatgaaagg ctcatcatct actaccaaaa tcttgagttt
     6661 tgccattttt gaatatttta ttattttaac aatttattga tttcccgaat caattggtcg
     6721 ggacgcagac cttttggtaa aaacaggtcg gccttgatcc atcgagaatt catgcccagt
     6781 tctgtttcaa aacttatgcc tgtttcggca gtaacagcag taaaaataat tacaggaaca
     6841 tcgggataca atcttttgat ttgatagctc agaataaaac cactatcttc attttccatc
     6901 atcaaatcaa ctatggccag atcgggtttg gtgcgttcta tgattttttg agcttctcgc
     6961 tggctttcgg ccgtaattac ccgaaatcca ttcttttcga gcaatacctg caactggaac
     7021 aagtaatcgg gatcatcatc gacaataaaa atcgttttct tatgagcttc catgatggta
     7081 atttttttat aatgaaattg ttaaactgaa ttggggttgc ggggtaaggt aattgtaaaa
     7141 attgttcctg taggcccttc ttctttattg ttattcgaca tgacgctaat ctggcctttg
     7201 tgcattttca caattccata gatgagcgga agtcccaagc cagtgccttt gcccagctct
     7261 ttggtagtgt agaagggagt aaatagcttg tccatatttt ccttctcgat gcctatgcct
     7321 gtatcggcaa ctttaataat aacttcatga agattgtctt taagggtgac agtaatttcg
     7381 ccgccttcgg gaggcatagc ttcaatcgca tttttttcga gatttgtcaa aacctgcatc
     7441 atctgttctt gatcaaggaa acagataggg tctttcattt ctgaaatcat tcttgttttc
     7501 acattatcgg gtttaaccac cgaatcgagg cttcgttgaa ctaattgttc cagatgggtc
     7561 tcagtcagat tgacttgatt tttacgtgca aaattgagta aaccacttac aattttctta
     7621 cagcgattgg cttgctccac aatcaattcc agatccttac gcaggggatc attctcaggc
     7681 cgttcttcca tcataatatt agcatacatt gtaataactc ccaaaggatt attgagttca
     7741 tgggcaatac cggcagaaag ctgacccatg tgtgccaatt tttccgattg ttttaaagcc
     7801 gctttggcat gagcaagttt ttcgttggtg atgttcaggt cattgatgga tttatgtaac
     7861 ttttcgatgg tatagggcag gcacatttcg ggctctgcaa agccctgagc aattgctatg
     7921 gcatgttcct cgcaagtgtc ataaccgcat gctccacagt tcaaataatc ttg
//
LOCUS       DAOORU010000111         7961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_11223_length_7961_cov_4.913230, whole genome shotgun sequence.
ACCESSION   DAOORU010000111 DAOORU010000000
VERSION     DAOORU010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7961
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11223_length_7961_cov_4.913230"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /locus_tag="PLF20_07510"
     CDS             <1..1508
                     /locus_tag="PLF20_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX80881.1"
                     /translation="HKPLLNLNHYWTISNKASLSTSAYFSYGKGGGNWAENFSGKNVF
                     EYQTPSGQVDWDAIARDNLTHQDIYITPQGDTLRHFAKNSQTLFLASHYWAGLISAYS
                     YKPNDQIILTTGIHARYFKSWLWEEIYDLLGAQYFIDDYAWAIESRQGRNILKKRGDT
                     TRIDNGAVVGLASGFVRANYEQGDLSGLLSFGLNYNTYQREDRYNYPTHPASSIVNKT
                     GYELKAGMAYAFNSQLRVYFNGGYTSRAPYYKFVFPNFNNNPAQNLKNEKITSLEVGM
                     GWQKNDFNLNLAGYYVAWQDKSLLSNEYKLIDNQTTTRSLVTGLDALHEGIEAEAHYS
                     ALKSNLNLSAFVSVGNWKWQNNVDAKIFNNENQLIDSVKVYAEGLYVGDAPQFQAGVS
                     ALIHFLNQFDFHLSYIWNDRFYADFDPALRTKPEDAQSWRIPGYGLTDVRLGFHFEMM
                     GMETELALACNNLFDVEYITRGNDGIHHNKETFRGYWGPGRTFKLSLEIKF"
     gene            2057..3514
                     /gene="dnaA"
                     /locus_tag="PLF20_07515"
     CDS             2057..3514
                     /gene="dnaA"
                     /locus_tag="PLF20_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOX80882.1"
                     /translation="MDRHETKTNEAREKWAKCMKVIEDNIPPASFKTWFIPIEPLKFE
                     NNVLTIQVPSQFFYEWLEEHYIDLLKKTIRKELGPDCRLEYSIIMDNCYPLSDNITMR
                     LPASNRNTIENPDVSIPIDINPDGLAREIPNPFIIPGLKKIKIQSQLIDTLSFENFVE
                     GDCNQLARSAGYAIAQNPGKTSFNPFFIYSAVGLGKTHLAHAIGLQSKVNFPDKTILY
                     VTAEQFQHQFTDAVRNNTSNDFMNFYQMIDVLLIDDIQFLAGKQKTQDVFFHIFNHLH
                     LKGKQIVITSDKPPIELAGIEDRLLSRFKWGLAADIQTPDFETRVAILQKKIYNEGLV
                     IPEDVVEYIAANITTNVRELEGALTSVVAQSALNKKAISLELARKIIDKFVICTTKEI
                     SMDYIQKVVCDYFNISIEQLKSKSRKRSIVQARQLVMYFAKEYTTSSLAAIGLQCGNK
                     DHSTVLHACRTVKNLFETDRTFKQYYTDIERKIKI"
     gene            3526..4503
                     /locus_tag="PLF20_07520"
     CDS             3526..4503
                     /locus_tag="PLF20_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOX80883.1"
                     /translation="MLLESIPQPVDIYEAHKRISPYIHRTPVITSKTLDELSQCNLFF
                     KCENFQKAGAFKSRGACNAVFSLDDDESAGGVATHSSGNHAQALARAALLRGIKSYIV
                     MPRTAPIVKIAAVKHYQGKITFCEPTLAAREETLKNLLQQTNAIEIHPYNNYSVIAGQ
                     ATATMEIFEEIGWPDFLVAPVGGGGLLSGSSLAVRYFSNHTKTLGAEPEGADDAYLSF
                     HSGMIVPSIHPVSIADGLLTSLGSRTFPVIIENVTEILRVSEEKIVKAMQLIWERMKI
                     IIEPSAAVSFGAVLQYPEIFHSSKTVIILSGGNVDLGCLPFAAIKENMR"
     gene            4500..5213
                     /locus_tag="PLF20_07525"
     CDS             4500..5213
                     /locus_tag="PLF20_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOX80884.1"
                     /translation="MSKGKLFLIPAPISINSPFGWDVKTLSIITEMRYFIVEEIRTAR
                     KLLRRVIPSFPIDECTFQILNEHTPPADIPTMLQPMMKGHDMGLMSEGGIPCIADPGA
                     VLVWMAHCKNIQVIPLPGSSSIFLALAASGFNGQNFCFHGYLPVEKIARKQRIIELEQ
                     KAKHQTQIFIETPYRNISLLGSIMQYCQPTTNLCIASGLLSPNEFIKTMEIKQWKAFT
                     PPIHRVPAVFLLGNVYLTI"
     gene            5302..6489
                     /locus_tag="PLF20_07530"
     CDS             5302..6489
                     /locus_tag="PLF20_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX80885.1"
                     /translation="MKLLQQKMSKYDIPQKAQKMGVYPYFRVIESEQDTVVKINGRDI
                     LMFGSNSYLGLTNHPMIKEAAKKAIDKYGSGCAGSRFLNGTLDIHIKLEEKLAEFVQK
                     DAAIIFSAGFLVNAGVIPTLTGRDDYIILDELDHASIIEGRRLSFSQAFKYRHNDMSS
                     LEKILKQCKPDKIKLIVVDGIFSMEGDIVDLPGIVKLAKQYNATVMVDDAHSLGVIGK
                     NGSGTASHFGLEEDVDLIMGTFSKSLASIGGFIASDFYTINYLKHNCRSLIFSASIAP
                     AATGSVLAALEIIKNEPERIEKLWENTHYAMKGFKELGFDTGRSETPVIPLYVRDDAK
                     TFMFTRMLLDEGVFVNPVVSPAVPSDSTLIRFSLMATHTYQQIDQALEACQKVGRKLN
                     LID"
     gene            6514..7668
                     /locus_tag="PLF20_07535"
     CDS             6514..7668
                     /locus_tag="PLF20_07535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOX80886.1"
                     /translation="MNTIEIKPVKNVKDLQQFVAFPNILYKGDPHYVPVIVEDGIVTL
                     SSKHNPIIKDCKTAYWMAFRDGKMVGRIAGIINNEFIYKWGKRYARFGWMDFIDDPRV
                     AAKLFETVENWAIQNGMEAINGPLGFTDLDTSGLLIKGFEYKATILGRYNYPYYQKYI
                     EDLGYRKDADWIEYQIDIPDAMPRKLEQIAHYVTQRHNLIPHTFKNINEAFPYVNQIF
                     DIVNQIYGLVYGYVPITKEIGDFYISRYKYLLDPELISIITDRADKVIAFGVAMPSYS
                     DVFIKSKGNMLRLKWLLFKGEHKKTDTVDMYYIGVRPGYIRKGISSVLMYEIGEKIIE
                     RGYRYAESNFEYESNFAVRSLWRHFSHRQHKCRRCYQKYFLSSTRSEDSI"
     gene            7680..>7961
                     /locus_tag="PLF20_07540"
     CDS             7680..>7961
                     /locus_tag="PLF20_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80887.1"
                     /translation="MAIEIKEISGRKEIKKFVCFPFPLYKGNPFWVPPLIFDEVNALD
                     PRKNPSFEFCEAKLWMAYKDGRAVGRIAGIINHRANQIWNERNARFGWLD"
BASE COUNT         2470 a         1665 c         1598 g         2228 t
ORIGIN      
        1 atcataagcc acttttaaat ttaaaccact attggactat cagtaataaa gcctctcttt
       61 caacttcggc ctatttcagc tatgggaaag gaggaggcaa ttgggccgaa aacttcagtg
      121 ggaaaaacgt attcgaatat cagacgcctt cgggccaagt cgactgggat gccattgcca
      181 gagataatct cacccatcag gatatctata ttacacccca aggcgataca ttgcgccatt
      241 ttgcaaaaaa ctcccaaacc cttttccttg cttcacatta ctgggcagga cttatctcgg
      301 cttatagtta taaacctaac gatcaaataa ttctcactac cggaatccat gcacgttatt
      361 tcaaaagctg gctatgggaa gaaatttacg acttgctggg agcacaatat tttatcgatg
      421 attatgcttg ggctattgaa agtcggcagg gaagaaacat tttgaaaaaa agaggagaca
      481 cgacgagaat tgataacggg gctgttgttg gactggcttc gggctttgta cgggcaaatt
      541 atgaacaagg cgatctgtcg ggattgctat cattcggact caactacaat acctatcaaa
      601 gagaggatcg ctacaattat cccacacatc cggcaagttc catcgtcaat aaaaccggat
      661 acgagctgaa agccggcatg gcgtatgcat tcaactcaca attacgagtt tacttcaacg
      721 gaggttatac ttcgagagcc ccttactaca agttcgtgtt ccccaacttt aacaataatc
      781 cagctcaaaa cctcaagaac gaaaaaatta cttcgctaga agttggaatg ggatggcaaa
      841 aaaacgactt caacctcaac ctcgcgggat attatgtggc atggcaggac aaatcactcc
      901 tttcaaacga atataaactg atcgataatc aaactacaac ccgctcgctg gttacaggtc
      961 tggatgcatt gcatgagggt atagaagccg aagcccatta ttcggcattg aaatctaatc
     1021 tgaatctttc ggcttttgtt tctgttggga actggaaatg gcaaaacaat gtggatgcaa
     1081 aaatattcaa caacgaaaat cagttgatcg acagtgtaaa ggtttatgcg gaaggtttgt
     1141 atgtgggtga tgcgccacag tttcaagccg gagtttcggc gctgattcat ttcctgaatc
     1201 aattcgattt tcacctgtct tatatctgga acgaccgatt ttacgccgat ttcgaccccg
     1261 ccctaaggac aaaacccgaa gatgctcagt catggcgaat tcccggatat ggattaaccg
     1321 atgttcgctt gggatttcat ttcgaaatga tgggaatgga aactgagctg gcgctggctt
     1381 gcaataacct tttcgatgtg gagtacatca cccgtggaaa cgatggaatt catcataata
     1441 aggaaacctt caggggctac tggggaccgg gaagaacctt taagttaagc ctcgaaataa
     1501 aattttaatc cttattgaca cctgacactt aaaacaatag gcgtcaatct cattgggata
     1561 taactattta tttattgata aacaggtagt taaggaataa ctccctgact gattctctca
     1621 ttatactcat ttataatcaa tcatctctat ttagaatgat tccaatatta aatttttcat
     1681 ttttggtttg cccatttgaa aaattccttt aatttagacg gtgggaaaaa ctccctccaa
     1741 atactttaat aaattgaaaa acaaatgatt atattaataa ttcattatta tgcctttatg
     1801 atagataaac ctttatttta acagaatatt tatctcttga gatgaataga agtgcaaaat
     1861 aaaactttga aaaccaaacc aaaacgagtt tcaagaaaag tgtatacaca aagaattgaa
     1921 aaatgcaagg aggaaaaggc ttttttttca aaaattttac acacaatttt gttgaagact
     1981 tttggagagg gggcacaaat gagcaaaatt gtatttattg aactgaatga taacttaaaa
     2041 atatcaacga atgtatatgg atagacacga aactaaaacc aatgaggccc gagaaaaatg
     2101 ggcaaagtgt atgaaagtca tcgaggataa tattcctccg gcaagtttca agacttggtt
     2161 tattcccatc gagccgctga agtttgagaa taatgtctta accattcagg ttccgagtca
     2221 atttttctat gaatggctcg aggagcacta tatagacctt ttgaaaaaga caatcagaaa
     2281 agaattggga cctgattgcc gtctggaata cagcatcatc atggataatt gctatccgtt
     2341 atccgataac ataaccatgc gtttgcccgc ttcgaatcgg aataccatcg aaaatcccga
     2401 tgtaagcatt cctatcgaca ttaatcctga tggcttggcc agagaaattc ccaacccttt
     2461 tatcatacca ggtttaaaga aaatcaagat tcaatcacaa ttaatcgata ctctgtcatt
     2521 tgaaaatttt gtagaaggcg actgcaacca attagctcgt tcggctggat atgccattgc
     2581 tcagaatccg ggcaaaacct cctttaatcc tttttttatt tacagtgctg taggtttggg
     2641 aaaaactcat cttgcccatg ctattggcct gcagtcgaag gtaaattttc ccgataagac
     2701 gatactttac gttactgccg aacaatttca gcatcagttt accgacgcag tacggaacaa
     2761 tacttccaac gacttcatga acttctacca aatgatcgat gttttgctga tcgacgatat
     2821 tcaatttttg gctggaaagc aaaaaactca ggatgtattc ttccacattt tcaatcattt
     2881 gcatttaaaa gggaaacaga tcgttattac ctccgacaaa ccgccgatag agcttgctgg
     2941 tattgaagac cgtctgcttt ctcgattcaa atggggtttg gccgccgata tacaaacacc
     3001 tgattttgaa acacgtgtgg caatacttca gaaaaaaata tataatgaag gccttgtaat
     3061 acccgaagat gtagtcgaat acattgccgc caacattacc accaatgtgc gtgaactcga
     3121 gggagctctt acatcggttg ttgcccagtc ggcactcaat aaaaaggcga tttcgctcga
     3181 gttggcacga aaaatcatcg ataaattcgt gatttgcact accaaggaaa tctctatgga
     3241 ttacattcaa aaagtggtat gcgattattt caatatcagt atcgaacagc tcaagagcaa
     3301 gtcacgcaag cgctccatcg ttcaggcacg ccagcttgtc atgtattttg caaaggaata
     3361 tacaacatct tctttagcag ctattgggct tcaatgtggc aacaaagacc actcgactgt
     3421 attgcatgcc tgccgaactg taaaaaatct gtttgaaacc gatagaacct ttaaacaata
     3481 ttatactgat attgaaagga aaataaagat ttaaaccgaa accgtatgct tctcgaaagc
     3541 attcctcaac ctgttgatat atatgaagct cacaagcgta tttctccgta cattcaccga
     3601 acacctgtaa ttaccagcaa aactctggat gaactaagcc aatgtaatct ttttttcaaa
     3661 tgcgagaatt ttcagaaggc aggggcgttt aaatcgcggg gagcgtgcaa tgctgttttt
     3721 tcgctcgatg atgatgaaag tgcaggggga gtagcaaccc attcgtcggg gaatcacgct
     3781 caggcccttg ccagagccgc attgcttcgc ggaataaaat cctatatcgt tatgccacgt
     3841 acggccccaa tagttaaaat tgctgccgta aaacattatc agggcaaaat aaccttctgt
     3901 gaacccactc ttgctgctcg tgaagaaacc ttgaaaaact tgcttcaaca aaccaatgcc
     3961 atcgaaatcc atccctacaa caattatagt gttattgctg ggcaagccac cgctacaatg
     4021 gaaattttcg aagaaatagg ttggcctgac tttttagttg cccccgtggg tggaggagga
     4081 ttattgagtg gttcgagctt agctgttcgt tatttttcga atcataccaa aacattggga
     4141 gccgaacctg aaggcgctga cgatgcttat ctctccttcc atagtggtat gattgtccct
     4201 tccattcatc ccgtaagcat tgccgatggt ttgctcacct cactgggctc gagaactttt
     4261 cctgttatta tagaaaatgt tactgaaatc ctgagggtta gcgaagaaaa aatcgtgaag
     4321 gcaatgcaat tgatctggga aagaatgaaa ataattatag aaccgtcggc tgccgtttcg
     4381 tttggggcgg ttcttcaata tccggaaata ttccacagtt ctaagactgt catcatcctt
     4441 tcgggtggaa atgtcgatct tggttgcctt ccctttgcag ccatcaaaga aaatatgaga
     4501 tgagtaaagg aaaattattt ttaattcccg cccctatttc catcaattcg ccctttggct
     4561 gggatgtaaa aaccctttcg attatcacgg agatgcgata ttttattgta gaagagattc
     4621 gcaccgcaag aaaactcctg cgcagggtca ttccctcctt ccctatcgac gaatgcacat
     4681 tccaaatcct taatgaacat accccaccgg cagatattcc cactatgctc caacccatga
     4741 tgaagggaca cgatatggga ttgatgagcg aaggaggaat tccttgcatt gccgatccgg
     4801 gggccgtttt agtttggatg gctcactgca aaaatattca ggttatccct ttaccgggtt
     4861 cttcttctat ttttttagcc ttagctgcat caggtttcaa cggacaaaat ttctgttttc
     4921 atggctacct gcccgtcgaa aaaattgcac gtaaacaacg aataattgaa ctggaacaaa
     4981 aagcaaaaca tcaaacccaa atctttatcg aaacgcccta ccgaaatatc tctctcttgg
     5041 gttcaatcat gcagtattgc caacccacca ccaatttatg cattgcttca ggcctactct
     5101 cccccaacga atttataaaa accatggaaa taaaacaatg gaaagcattc acccctccta
     5161 ttcatcgagt acccgcagtg tttctacttg gaaatgttta tcttacgata tgagggagca
     5221 ataagaaatt gccttaagta aagtttccat atatttgcca gtttttgtcg aatcttaact
     5281 aaaacctatt atcaagacag tatgaagcta ttacagcaga agatgtcgaa gtacgacatt
     5341 ccacagaaag cccaaaaaat gggtgtgtat ccttactttc gggtcattga atcggagcag
     5401 gacacagtgg tgaaaatcaa tggaagggat atattaatgt ttggttcgaa tagttacctc
     5461 ggactcacca atcatcccat gattaaggaa gccgcaaaga aagccataga taaatatgga
     5521 agtggatgcg ctggttcaag atttttgaat ggtacgcttg atattcatat caaactggaa
     5581 gaaaaactag cagaattcgt tcagaaggat gcagccatta ttttcagtgc aggtttttta
     5641 gtcaatgcag gagtcattcc cactttaact ggacgtgacg attatattat cctcgacgaa
     5701 ttagaccatg cctcgatcat agagggtcgc cgtctatcgt tttctcaggc ttttaaatac
     5761 cgccataatg atatgtcttc gctcgagaaa atcctcaaac aatgtaaacc cgacaagatc
     5821 aaacttattg tggtagatgg tatcttcagt atggaaggag acattgtaga tttacctggc
     5881 atcgtaaaac tggcaaaaca atataatgct acggtaatgg tcgatgatgc tcattcgtta
     5941 ggagtgattg gcaaaaatgg ttcgggcact gccagccatt tcggattgga agaagatgtt
     6001 gacctcatca tgggtacatt cagcaaaagc ctggcttcga taggaggttt catcgcttcc
     6061 gatttttata ccatcaatta tttgaagcac aactgtcgtt cattgatatt tagcgccagc
     6121 attgctcctg ctgctacagg tagcgttttg gcagcactcg aaatcattaa gaatgaaccc
     6181 gaacgcatcg aaaagctttg ggaaaatacc cattacgcca tgaaagggtt caaggaatta
     6241 ggtttcgata ctgggaggtc ggaaactccc gttatccccc tttatgtaag agacgatgca
     6301 aaaaccttca tgtttacacg aatgttgctc gatgaaggag tttttgtaaa tcccgtagtt
     6361 tcgcctgctg tgcccagcga ttcgaccctc atccgttttt cgctgatggc cacccatacc
     6421 tatcaacaaa tcgaccaagc cttggaagct tgccaaaaag taggcagaaa gctcaacctc
     6481 atagattgaa gtcaaccttt aggatgcctc accatgaata caatagagat taaaccggta
     6541 aaaaacgtca aggatcttca acagttcgtt gctttcccca acatactcta taaaggagat
     6601 cctcattatg tacccgttat cgtcgaagat ggtatagtaa ccctttcttc caaacacaat
     6661 ccaattataa aagattgcaa aacagcctat tggatggctt ttcgggacgg gaaaatggta
     6721 ggacgtattg ccggaatcat taacaatgaa ttcatttaca aatggggaaa gcgttacgct
     6781 cgtttcggat ggatggattt tatcgacgac cctcgggtag ccgccaaact cttcgaaaca
     6841 gtagaaaatt gggctattca aaatggaatg gaagccatca atggtccact tggctttacc
     6901 gatctcgata cctcagggtt gctcatcaaa ggtttcgaat ataaggccac aatattgggt
     6961 agatacaatt atccctacta tcaaaaatat atagaggact tgggctatag aaaagacgcc
     7021 gactggatcg aatatcagat tgatattccc gatgccatgc ctcgtaaatt ggaacaaata
     7081 gctcattacg ttactcagcg ccataatttg ataccacata ccttcaaaaa tataaacgaa
     7141 gccttccctt acgtcaatca aatcttcgat atcgtaaacc aaatatatgg cctggtttat
     7201 gggtatgtcc ctattaccaa agaaattggt gatttctata tttcccgtta taaatatctt
     7261 ctcgatccag aattaatttc cattattacc gatcgagccg ataaagtaat cgcctttggg
     7321 gttgctatgc cctcatacag cgacgtgttc atcaaatcga aagggaatat gcttcgtctt
     7381 aaatggcttc tttttaaagg agaacataaa aaaaccgata cggtcgatat gtattatata
     7441 ggagttcgtc cgggatacat tcgcaaagga atcagttcgg tattgatgta tgagattggg
     7501 gaaaaaatta ttgaacgtgg ttatcgttat gcagaaagca acttcgaata tgaaagcaat
     7561 ttcgcggtgc gatcgctttg gagacatttt tcacatcgtc agcataaatg ccgcagatgc
     7621 tatcagaaat atttcttaag tagcacacgt tcagaagata gcatataaaa cttattctta
     7681 tggcaatcga aatcaaagaa atttcaggac gaaaagaaat caagaagttt gtatgctttc
     7741 catttcccct ttacaaaggg aatcctttct gggttccccc tcttattttc gatgaagtga
     7801 atgctctcga cccgaggaaa aatccttcct tcgaattttg tgaggccaaa ttatggatgg
     7861 cctataagga tggacgagca gtgggacgca tagccggaat catcaatcat agggctaatc
     7921 agatatggaa cgaacggaat gcccgttttg gctggctcga t
//
LOCUS       DAOORU010000112         7951 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_11246_length_7951_cov_4.968845, whole genome shotgun sequence.
ACCESSION   DAOORU010000112 DAOORU010000000
VERSION     DAOORU010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11246_length_7951_cov_4.968845"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..1352)
                     /gene="metK"
                     /locus_tag="PLF20_07545"
     CDS             complement(12..1352)
                     /gene="metK"
                     /locus_tag="PLF20_07545"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOX80888.1"
                     /translation="MGYLFTSESVSEGHPDKVADQISDALLDEFLRWDPDSKVAVETF
                     VTTGLVVVGGEVKTSGYVDVQEIARQTIRRLGYTKAEYRFDSDSCGVISAIHEQSPDI
                     FQGVAKSNPLEQGAGDQGMMFGYACKEMDNYMPMPIELAHILVQQLAAFRREGKEMPY
                     LRPDAKSQVTIEYNDEGQPVRIDTIVLSTQHDEFIIPENSSPNAKREAENCMQKKIIE
                     DVRNILIPRVRETIPARVDQLFKNGYKLYVNPTGKFIIGGPHGDTGLTGRKIIVDTYG
                     GKSAHGGGAFSGKDPSKVDRSGTYAMRHIAKNLVAAGACDEVMVQVAYAIGIAEPIGL
                     YINTFNTCKAKNKDGKIFTDAELARIVLEIFDLRPYSIITRFGLKNPIYLPTAAYGHF
                     GRDPYTQEVEVFYQNDHTYVKTENGKERYFKKVEFFPWEKIDYVDILKKEFNLD"
     gene            complement(1673..2581)
                     /gene="gldA"
                     /locus_tag="PLF20_07550"
     CDS             complement(1673..2581)
                     /gene="gldA"
                     /locus_tag="PLF20_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HOX80889.1"
                     /translation="MSVKVDRVTKIYGSQRVLDDVSFQVEKGQITGLLGPNGAGKTTL
                     MRIITCFIPPTEGRVLVNGINTMENPVAVRKMLGYLPENNPLYPDMYIREYLEFIAGL
                     YKVSLPKKRIEEIIDLTGLEPEISKTIGKLSKGFRQRVGLAQALIPDPEVLILDEPTS
                     GLDPNQIIEIREVIKKASANKTIIFSTHIMQEVEALCQRVIIINKGKIVADDLTANLS
                     NLVSSSETILAGFDRKVEIHLIRQINGVIAVKAKENNVFEIKAKPNTDIRPALFKFAV
                     DHHFTVLSLQQSEKSLEEIFHEITQK"
     gene            3352..3807
                     /gene="rplM"
                     /locus_tag="PLF20_07555"
     CDS             3352..3807
                     /gene="rplM"
                     /locus_tag="PLF20_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOX80890.1"
                     /translation="MNTLSYKTISANKQTVKKEWVLIDAENLVVGRLASEVAKILRGK
                     NKPYYTPHVDCGDHVVIINAEKIRFTGKKMDDKKYVSYSGYPGGQRSTTPKKLLKRVP
                     ERVIENAIRGMLPKNRLGRELFRNLHVYAGSSHPHQAQSPKQIKIIEAE"
     gene            3811..4197
                     /gene="rpsI"
                     /locus_tag="PLF20_07560"
     CDS             3811..4197
                     /gene="rpsI"
                     /locus_tag="PLF20_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOX80891.1"
                     /translation="MDVINTSGRRKTAIARIYMSQGNGKIMINGRELNDYFKTNVLIY
                     KVLQPLELTNNQGKYDIRVNLEGGGITGQAEALRLAIAKALVEMNAEYRPILKDKGLL
                     TRDPRMVERKKPGRPKARKRFQFSKR"
     gene            4312..5217
                     /gene="rpsB"
                     /locus_tag="PLF20_07565"
     CDS             4312..5217
                     /gene="rpsB"
                     /locus_tag="PLF20_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009162128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOX80892.1"
                     /translation="MSRVTFEELLEAGVHFGHLRRKWNPNMAPYIFMERNGIHIIDLY
                     KTIAKLEEATAAVKQMAKSGKKILFISTKKQAKEIVAEQAKRVNMPYVVERWPGGMLT
                     NFATIRKAVKKMAAIDKLMETENFLTMSKRERLQITRERAKLEKNLGSIADLTRLPSA
                     IFVVDILKEHIAVAEARKLNIPIIAMVDTNSNPNLVDFPIPANDDASKSIQLIVKTIA
                     DAIEEGLNERRFEKDQVEQEIEESYDETAEKKKLELDTSLEEEELVARNKLHKGTPAG
                     EDEEASTTKRPRKIISKSSRSDSHR"
     gene            5254..6087
                     /gene="tsf"
                     /locus_tag="PLF20_07570"
     CDS             5254..6087
                     /gene="tsf"
                     /locus_tag="PLF20_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOX80893.1"
                     /translation="MPISASDVNKLRQITGAGIMDCKHALEESNGDFDQAIDILRKKG
                     QKIANKRADRDTSEGMVKAATNPEKTYGAIIMVNCETDFVASNSEFTQLVKKLMDKII
                     EKKPKSLDEARNLKLDDRTFDDHLTELIGKTGEKMEFSIFQYIEAPLVDAYNHHNNRL
                     ATIVGFNKNDREEFATIAHEVAMQIAAMNPIAIDKDDVDPKIIEHEMEIGREQARTEG
                     KAENMLDKIAEGKINRFFKESTLLNQDFVSDQKKTVGQYISEFDKELKVTKFYRLILG
                     E"
     gene            6450..7739
                     /locus_tag="PLF20_07575"
     CDS             6450..7739
                     /locus_tag="PLF20_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80894.1"
                     /translation="MLKKNKKIIAISGGVVLILLIILISTRNKPADKELIVKVQRGPF
                     KITVTTTGELEAKHSEKILGPDGLNEVGIWRTTIEDIIPDGSIVDSGQYVARLDQTEI
                     SNKIKDMETELEKLETQVIKTRIDTSIEMRAARDELINLKYALEERRIILEQSAYEPP
                     ATIRQAEINLEKAERAYEQAIKNYQLKLDKNRANMQDVQASYLQAKRRLENLLKVREG
                     FVVKAPKSGMLIYRRNWDGKKMGVGAQISTWDNIVATLPDLTKMISKTYVNEIDISKV
                     KTSQKAEISIDAFPNKKLTGLVDEVANIGEQIQGSNAKVFEVVINVNEFDSILRPAMT
                     TKNVIITREFQNVLYLPIEAVHTEGKVTFVYKDNKKVEISTGESNENYIIVKNGLKEG
                     DAVYISIPKETDHWPVARLSKSESTDKPKDGRDKSNG"
     gene            7732..>7951
                     /locus_tag="PLF20_07580"
     CDS             7732..>7951
                     /locus_tag="PLF20_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX80895.1"
                     /translation="MDKKSHSQPGIKYIHILRIAGDALMVNKTRTLLTALGIIFGVAA
                     VIAMMAVGNGARKEILDQMKLVGVNNIII"
BASE COUNT         2638 a         1603 c         1552 g         2158 t
ORIGIN      
        1 attttctggg atcaatccag attgaattcc tttttcaata tatctacata atcgattttt
       61 tcccaaggga agaattctac ctttttaaaa tatctttctt tgccattttc ggttttaaca
      121 taagtgtggt cgttctgata aaaaacttcc acttcctgag tatagggatc gcggccaaaa
      181 tggccataag cggcagtggg aaggtaaatg ggatttttga gtccaaagcg agtaataatg
      241 gaataaggtc gcaagtcgaa tatttccaat acgattcgtg caagttcagc atcggtaaat
      301 atttttccat ccttattttt agctttacat gtattgaatg tattgatata caatccaata
      361 ggctcggcaa tccctatagc ataagccact tgtaccatca cttcatcgca agcaccagca
      421 gccacgaggt ttttggcaat atgccgcatg gcatacgttc ccgaacgatc caccttcgaa
      481 ggatccttac ccgagaaggc accacctcca tgtgcgcttt tcccgccata ggtatccaca
      541 ataatcttcc tcccagtgag tcctgtatct ccatgaggac ctccgataat aaattttcct
      601 gtaggattga catagagctt atagccgttt ttgaataact gatccacccg agcgggaatt
      661 gtttccctta cccttggaat caggatatta cgtacatctt cgatgatttt cttctgcatg
      721 caattttcgg cttctctttt tgcattgggg ctcgaattct caggaatgat aaattcgtcg
      781 tgttgggttg aaagaacgat agtgtcgatt cgaacgggtt gcccttcgtc attgtattcg
      841 atagttacct gcgacttagc atcggggcga aggtagggca tttctttacc ttcccttcga
      901 aatgctgcca gttgctgtac aagaatatgc gccagttcaa ttggcatggg catataattg
      961 tccatttcct tgcaagcata gccaaacatc attccttggt cgccagcacc ctgctcaagt
     1021 gggtttgatt tggctactcc ctgaaaaata tcaggtgatt gctcatgaat tgcactaatc
     1081 actccgcaag agtcggaatc aaaacggtac tcggctttgg tatatcccaa acggcgaata
     1141 gtttgccgtg ctatctcctg gacatccaca taacccgagg tttttacttc gcctcccacc
     1201 actaccaaac cggtagttac aaaggtttcg acggcaactt tcgaatcggg atcccaacgt
     1261 aaaaattcat cgagcaatgc atccgaaatt tgatcggcta ccttatcggg atggccttct
     1321 gacactgatt ctgaagtaaa taaatatccc attatgtctt taatttattg gttgaacatg
     1381 taaatgggga aattgattga gaaggaaaaa aatattgttt tagcattttt ttctgtggtt
     1441 gcaatcaatc aaatctctcc acttgctttt aataaatgaa ctgcaaaaat agaataaatc
     1501 tttcgacaaa aaatatccca accaaagatt ttactctttc taaacataaa atattgatat
     1561 atggggaaat atcctattca tcgaaaaaac acaaattcaa aagttgttga cttgtgctgt
     1621 aaccctgaaa agaaaagggt gaatttaaaa tcatctaact aatggatgag aattactttt
     1681 gggtaatttc atgaaatatt tcttcgagac tcttttcgct ctgttgcaat gataacaccg
     1741 taaaatgatg atcgacggca aacttgaaca gtgcaggccg aatatccgta ttgggtttcg
     1801 cctttatttc aaacacattg ttttcttttg ccttcaccgc aattactcca ttgatttgac
     1861 gaatgaggtg aatttcgact tttcggtcga atccggcaag gatcgtttca ctgctcgata
     1921 caagattcga cagattagcc gtaagatcat cggctacaat tttgcctttg ttgatgatga
     1981 tgaccctctg gcataatgct tccacctcct gcatgatgtg tgtggaaaag atgatggttt
     2041 tattggcgct tgccttttta atgacttccc tgatttcgat aatttgatta ggatcgaggc
     2101 ctgaagtagg ttcatccagg attaagactt cagggtcggg aatcaaagct tgtgccagtc
     2161 caactctctg ccggaaacct ttcgacaatt ttccaatagt cttcgaaatt tcgggttcga
     2221 gccctgttaa atcaataatt tcctcaatgc gtttctttgg taaagagact ttatacaagc
     2281 cggcaataaa ttcaagatat tcacgaatat acatatcggg gtacaaagga ttgttttcgg
     2341 gcagataacc aagcatcttt cgcactgcaa ccggattttc catagtattt attccattga
     2401 ccaaaactcg accttcggta ggaggaataa aacaggtaat gattcgcatc aacgtagttt
     2461 ttccggcccc attaggacca agcaggccag taatctgacc cttttctacc tgaaaagaaa
     2521 catcatcaag gactctttgc gagccataaa tctttgtaac ccgatctacc tttactgaca
     2581 taatttgcca atttctaaca atacgaaagc aaaattaagt gaaacgtttc agatggaata
     2641 ggatcaaatc tttatcaaac tgtctatcgc catgtaaaaa ccaacaaagg cgaaacttct
     2701 ttcagcattt aaccatatta aggataactg ttccacgagg tgtggaatac atccttcagg
     2761 ttcggctcca aaattcaaat ccatacattt tacggcagaa aaatacccgt cgagtaatct
     2821 caagcgaata gcccagcttg aatacttgaa taaatgattg atcctttatt cgttaaaaac
     2881 agataagccc tcggcaatta ttattcaaaa ctttgtttgc ttctgaacaa taatgaaata
     2941 caacttgtta aaaatcaatg gataacggat cttcccaatc gacttgattt catataagtg
     3001 gaatttgtga aactgaaggg acaaaaatca tcctcacaac gaataaggca tgttcctagt
     3061 ataaataata aggaaattaa atacgccagg ggccaattcc cttacactgc ttatccgtcc
     3121 acattgtcta taggaagtta cttgatagag tgctaaacat tttattctat ttattataac
     3181 tttgcacatc acttacagtg tattaacaaa acaaattaat aggcggatta ccagacctgc
     3241 aacaatattt ataaaaaaat ttgagagtat aggtttgttt aataagaaat attgccatac
     3301 ctttgcaccc catttttaag cttttcaaag tataagaaaa tgaactcatc gatgaatact
     3361 ttaagttata agactataag tgccaacaaa caaactgtta aaaaggaatg ggtacttata
     3421 gatgctgaaa acctggttgt aggccgattg gcgtcggaag ttgccaaaat cctgagaggc
     3481 aagaacaaac cttattatac acctcatgta gattgtggtg atcacgtagt tatcatcaat
     3541 gccgaaaaga taagatttac ggggaaaaaa atggatgata agaaatatgt gagctatagt
     3601 ggctatcctg gtggacaaag gtcaactaca cccaaaaaat tattaaaacg cgttcccgag
     3661 cgggttatcg aaaatgccat cagaggaatg cttccgaaaa acaggcttgg cagagaatta
     3721 ttccgcaatt tgcatgttta tgcaggtagt tctcatccgc accaggctca atcacccaaa
     3781 cagatcaaaa tcatagaagc cgaataaagt atggacgtca tcaacacatc aggtagaaga
     3841 aagaccgcaa ttgccagaat ttacatgtcg caaggcaatg gaaaaattat gataaatggg
     3901 cgagaactga acgattattt caaaaccaac gttttgattt acaaagtact acaacctctc
     3961 gaactcacca ataatcaagg caagtatgat attcgggtta atttggaagg aggtggtata
     4021 acaggacaag ccgaagccct taggcttgcc attgcaaaag cattggtaga aatgaatgcc
     4081 gagtatcgcc caattcttaa agataaagga ttgttgaccc gtgatcctcg aatggtggaa
     4141 cgcaaaaaac ccgggcgtcc aaaagcccgt aagcgtttcc agttttcaaa acgataatgt
     4201 tatttgttac ggggaaattg tgtttagtat ccaaatccct gcgattgctt tgcaaagcac
     4261 tacttaggga ttgattttag ttgaaagtaa accattaaat atctaaattt catgtcaaga
     4321 gttacattcg aagaattatt agaagccgga gtgcatttcg ggcatctgcg tcgtaaatgg
     4381 aatcctaata tggctcccta catttttatg gaacgtaatg ggattcatat catcgacctg
     4441 tataaaacca ttgcaaaact cgaagaagca accgccgcag tgaaacaaat ggctaaatcg
     4501 ggcaaaaaga tactttttat ttccacaaaa aaacaagcta aagaaatcgt agccgaacag
     4561 gcaaaacgag tcaatatgcc ttatgtagtc gaacgctggc ctggcggcat gctcaccaat
     4621 tttgccacca ttcgcaaggc ggttaaaaaa atggctgcca tcgacaagct gatggaaaca
     4681 gagaatttcc ttaccatgtc gaaacgcgag cgccttcaaa ttacccgaga aagggcaaaa
     4741 ctcgaaaaga atcttggctc catcgccgat ctaacccgtc tcccatcagc catctttgtc
     4801 gtagatatcc tcaaagaaca tatcgctgta gctgaagcca gaaaattaaa cattcccatc
     4861 atagctatgg tagataccaa ttcaaaccct aatctggtag atttccccat tccagccaat
     4921 gatgatgctt caaaatcgat tcaactgatc gttaaaacca tagccgacgc tatagaagaa
     4981 ggcctcaatg aaagaagatt tgaaaaagat caggttgaac aggaaattga agaatcgtat
     5041 gatgagactg ccgaaaagaa aaaattagaa ctcgatactt ccttggagga agaagaactt
     5101 gtagccagaa acaaacttca caaggggact ccagccggtg aagatgaaga agcttcaacc
     5161 accaagcgtc cacgaaaaat tatctcgaaa agttcaagat ctgactcgca tagataattt
     5221 ctcaagaaag aaaataattc aaaccaatta attatgccaa tatctgcttc agatgttaac
     5281 aaactgcgcc agataacagg tgcaggaatt atggactgca aacatgcact cgaagaatcg
     5341 aatggcgatt tcgatcaagc cattgatatt ttacgaaaaa aaggacaaaa gatagccaat
     5401 aaaagagccg accgcgatac ttcagaaggc atggtaaagg cagccacaaa tccagaaaag
     5461 acttatggcg ccatcattat ggtgaattgt gaaaccgact tcgtggcttc aaattccgaa
     5521 tttacacagt tggtaaaaaa actcatggat aaaattatcg agaaaaaacc caaatcactc
     5581 gatgaagccc ggaatctaaa actcgacgat cggactttcg atgaccactt aaccgaactc
     5641 ataggaaaaa caggcgaaaa aatggagttc tccatttttc aatatatcga agcccctctg
     5701 gtagatgctt acaaccatca taacaaccgt ctggcaacca ttgtaggatt caataaaaat
     5761 gacagagaag aattcgctac cattgctcat gaggtggcta tgcaaattgc agcaatgaac
     5821 cctattgcca tcgataaaga tgacgtagat ccaaaaatta tcgaacatga aatggaaatt
     5881 ggtcgcgaac aggctcgtac cgaaggaaaa gccgaaaata tgctcgataa gattgccgag
     5941 ggaaaaatca atcgcttctt caaagaaagt actttgctaa atcaggattt cgtttctgat
     6001 caaaagaaaa cagttggcca atacatcagt gagttcgata aagaactaaa ggtcacaaaa
     6061 ttttatcgcc tcattttagg cgaataaatc atcctatttt ttgtccaaaa agcgggtctc
     6121 acattgctga aatccgcttt tttatttaaa tttggatact ttcccagatc attttctgta
     6181 acatgatcga ttcagaatat ccttaattta taacaaaatt tataacaaac gagtacaatt
     6241 ctctctatat tctactttat ggctaatttc agtatttcag tatttattag cttgaaatag
     6301 attccctatc tttcgacttg ctgtgttata aattccagct gaattcatac tcaccgagat
     6361 gtatcagaat tgtaccgaaa gaaattctct gttctctttg acttagttcg acgtaaatga
     6421 gtaatattgc aattcgaaaa tcaaacgaga tgttgaaaaa gaacaaaaaa atcatcgcta
     6481 ttagtggggg agtggtgttg atattgctca ttattttgat ttccactcgg aataaacctg
     6541 ccgacaagga attgatcgta aaagtgcaaa gaggtccttt taagattacg gtgacaacta
     6601 ccggggagct tgaagccaag cattcggaaa aaatcctagg tcctgatgga ttaaatgaag
     6661 tagggatatg gcgaaccact atcgaagata ttattcccga cgggtcgata gtagattctg
     6721 gtcaatatgt agccaggctt gatcaaaccg aaatcagcaa taagataaaa gatatggaga
     6781 ccgagcttga aaaactcgag actcaggtga taaaaactcg aatagataca tctatcgaaa
     6841 tgcgtgctgc aagggacgag ttgattaatt taaaatatgc tctcgaagaa agaaggatta
     6901 ttctcgaaca atcggcctac gaaccgccgg caaccatccg tcaggcagaa atcaatctcg
     6961 agaaagccga aagagcttac gaacaagcca taaagaatta tcagctcaaa ttggataaga
     7021 atcgtgccaa catgcaggat gtacaggctt catatcttca agctaaacgc cggctcgaga
     7081 atttactcaa agttagagaa ggctttgtgg taaaggcacc caaaagcggt atgctgattt
     7141 accgccgcaa ctgggatggt aaaaaaatgg gtgtgggcgc tcaaatcagt acatgggaca
     7201 acattgtggc cacccttccc gatctcacaa aaatgatttc aaaaacttac gtcaacgaaa
     7261 tcgatatctc gaaagttaaa accagccaaa aagccgaaat tagcatagat gccttcccca
     7321 ataaaaaact cacgggccta gttgacgaag tagccaatat tggcgagcag atacagggat
     7381 caaatgccaa agttttcgaa gtagtgatta atgtaaatga attcgacagc atattgcgtc
     7441 ctgccatgac aaccaaaaac gttataataa ccagagaatt tcagaatgta ttgtatttgc
     7501 ctatagaggc tgttcataca gaggggaaag ttacctttgt atataaagac aataaaaaag
     7561 tcgaaatttc tacaggcgaa agcaacgaaa attatatcat cgtaaaaaat gggctaaaag
     7621 aaggggatgc agtttacatc agcattccca aagagactga tcactggcct gttgcccgct
     7681 tgagcaagag tgaatccaca gataagccga aggatggaag ggacaaatca aatggataag
     7741 aaaagtcatt cccagccagg aatcaaatac attcacattt tacggattgc aggtgacgcc
     7801 ctgatggtaa acaaaaccag aactctgctt actgcgctgg ggattatctt tggtgttgcc
     7861 gccgtcatcg caatgatggc tgtgggcaat ggagcgcgta aagaaattct ggatcaaatg
     7921 aaattagttg gggtcaacaa tatcatcatt a
//
LOCUS       DAOORU010000113         7749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_11625_length_7749_cov_4.805563, whole genome shotgun sequence.
ACCESSION   DAOORU010000113 DAOORU010000000
VERSION     DAOORU010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7749
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11625_length_7749_cov_4.805563"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3231)
                     /locus_tag="PLF20_07585"
     CDS             complement(<1..3231)
                     /locus_tag="PLF20_07585"
                     /inference="COORDINATES: protein motif:HMM:NF039534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HOX80896.1"
                     /translation="MNSFSKITIAFCFLLMLFLQNVVLGQDTWIRKSDFAGSGRKGAV
                     ACTIGDKSYIGMGFDGIYHKDFWEYDPAIDSWIQLTDMPGQARAYAACFALGNKIYIT
                     TGYNGGFLLDTWVYNTTNHTWSAGINFPGGARQGCISTVIGDKAYVGLGYDGAYRKDF
                     YEFDPVSNSWTARNNFGGQGRIYASAFSIGTKAYVGLGYNGSYLSDFYVYDRVSNTWS
                     PINALSHGRSGAVGFALNQKGYIALGDDGTNLLNDLWEYDVNANLWSRKSDFPALPRE
                     NSVSFAYSDAAYISCGGDNATGYFRDLWKWQDDHCTLSVTATKKNVSCFGEADGSIHL
                     TTTGGIEPLIFTWNSGDNTSHISGLLPNTYQVTVRDEENCKLIKRFTITQPPALIFGK
                     DTSQWFLPRKGGGAAHDEGRCIVADEAGNIFLAGTFGGNAVFGSTSLTSTGGKDIFLA
                     KYNHLGNLQWIQQIGGSLDDEVTGIGLDSIGNIFLSGSFRVMALFGGNQYFAQGESDA
                     FLAKYSPTGILKWVQTGGGNFEDKALALYVVSDGSCFVTGYFQGNADFQGHNLISSGG
                     EDVFIARYNGAGVLDWIQQGGGLSPDRGHAVIYTIADEVVVTGIFQGNATFGNSSVTS
                     AGDNDIFIARYDRYGNLLSLQSSGGIADDQPFGLDADEQNNIFISGYFGATATFGGVN
                     LTCAGSKDVFVVKLNPSGNAIFAKRAGGSGEDIAYGLVYTPSGQIYITGKFSGSSQWD
                     SFDLVSSGGSDIFICRLDSAGVFRMVKSTGNIGDDVSYDLTLDKQYNPIFIGNFTNSL
                     LLAGTTLISSGQSDMIFAGLKESTFYRAPTIIPVTCESGNDGSIEVYIAGGTPDYTFQ
                     WSNGATTQNINEIPEGDYSLSISDTKGCTKDTSFVVSSQFTLPQAPVSAIVNRQGFCA
                     DDPGNITLTAIGGSGNNLVWFTGECGGDSIGTGTSITIPSPDTTTVYFARWENPCGVT
                     TCANIVVNVLPLPTPPNSIQIIPSTACEGAGSVILTATGGSGEVFRWYEGSCNGPLIA
                     TGNPAVIPAPSVSTTYFGRWETTCGVSICLSGSVTVLALAKPVTAVT"
     gene            complement(3770..4666)
                     /locus_tag="PLF20_07590"
     CDS             complement(3770..4666)
                     /locus_tag="PLF20_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80897.1"
                     /translation="MTGNYRRETKRIIFKSENFTEDDIVGIIMNLEQEYDQNNRLVME
                     RRYDNEGNLEATMSFQYDDSGNLIEQSEIDSSGEFAQKLTFQYDSMSRLIEKDIFYVD
                     ESFDREIYHYNSNNLLDKVEFINYDGEREQITEKRYNNDKLIEENTYDDSVQFTSSKK
                     WSYDDLGREVKFENETEDQYILSESVYDDEGRLILRKVYGKNGNLFETWERIYNPNPY
                     IEEYWETNRFGRSRYHVNYDENNRELSRQVTNENGEIMLEVERLYRPDGLLDRQRIII
                     HGSGMRPSQFEEYRNFYLPLNQ"
     gene            4987..7095
                     /locus_tag="PLF20_07595"
     CDS             4987..7095
                     /locus_tag="PLF20_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOX80898.1"
                     /translation="MPVLCLSIVVGFSSCTRTEKITYPETKRVDSVDEYFGQKILDPY
                     RWLENDTSAETSEWVKAQNEITNAYLEKIPFRKSLKERFTQIWDYPKYGIPFSKGNLY
                     FYFKNDGMQNQSVLYVQEGLEGEPRVLLDPNRLSEDGTVAIASFSVSNDARFLAYSIA
                     KAGSDWNEIYVMDINTRQNLPDHITWIKFSGMSWKGDGFYYSRYEAPKPGEELSIKNQ
                     NQRVFYHKLGSDPTKDELIWEDPQHPLRTFGASVTEDERFLIISESESTYGNKLHIKD
                     LQKPASSYIHIGDSFDWEFWVVDNLNDDLIVVTNYLAPRSQAVSINFSHPEISSWQTL
                     IPEKEEVLEGIYLANGKIISQYLKDACNHLYSHNLDGQLIDEIPLPGIGTIGGFSSEK
                     EKNIAFFAFTSFTYPTTIFKFDVEKNQTEVFRKPDIKFNSEDYTTEQVFYTSKDGTKV
                     PMFIVHKKNFLKNGKNPTLLYGYGGFNISLTPSFSISLIPFLENGGVYAEANIRGGGE
                     YGEEWHQAGTKMKKQNVFDDFIAAAEYLISNKYTSPKKLAINGGSNGGLLIGAVLNQR
                     PDLFAVAVPEVGVMDMLRYHKFTIGWAWAYDYGTAEDSEEMFHYLLSYSPLHNIKLGT
                     KYPAVMAMTADHDDRVVPAHSFKYTATLQAAQAGDAPILIRIESKAGHGAGRPTSKII
                     DNVTDKFSFIMYNLGMSPKF"
     gene            7215..>7749
                     /locus_tag="PLF20_07600"
     CDS             7215..>7749
                     /locus_tag="PLF20_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80899.1"
                     /translation="MTIKPGDKVRFLSSSGGGIVTRVIRPGIVAVEIEDGFEIPTAIN
                     DLVKIEETSPAARFFADNDPFLSQKDNESKTEPAVSPPFKSEQNASPLNESYIEKLPA
                     SRKSTINPEGIYLAFEPHDQNLFIIGYLDLCIVNFSSYDVLYTFSHKSPKGGYVGRDY
                     GSIPPYSKVIIDIIQREE"
BASE COUNT         2314 a         1623 c         1515 g         2297 t
ORIGIN      
        1 ggtaactgct gtaaccggct ttgcaagtgc taaaaccgtg acgctacctg aaaggcaaat
       61 ggaaacccca caagtagttt cccaacgtcc gaaataagta gtagaaaccg aaggagcagg
      121 aataactgct gggtttccgg tagctataag aggtccatta catgaaccct cataccaacg
      181 gaatacctca ccacttccgc cggtggctgt aaggattacc gaacctgcgc cttcacaagc
      241 cgtagaagga ataatttgaa tactgttggg tggtgttggt aaaggaagaa catttaccac
      301 aatattggca caagtggtga caccgcaagg attttcccag cgagcgaagt aaactgtggt
      361 ggtatccggc gaaggaatgg taatggatgt tcctgttcct atagaatcac ctccgcattc
      421 acctgtaaac caaacgagat tgttgccgct tcctcctatt gcagtaagag taatgttgcc
      481 aggatcatcg gcacaaaagc cttgcctgtt cacaatagct gaaaccgggg cttgtggtaa
      541 agtaaattgg gatgaaacca caaaagaggt gtctttggtg catccctttg tatcactaat
      601 agaaagtgaa taatctcctt cgggtatctc gttgatattt tgagttgttg caccattcga
      661 ccattgaaag gtataatcgg gtgtaccacc ggcaatgtat acctcgatag atccatcatt
      721 accactttca caagtaacgg gaattatggt tggtgctcga taaaaggttg actctttcaa
      781 tcctgcaaat atcatatcgc tctggccaga actgatgaga gttgtgcccg ccaataatag
      841 gctgttagta aaattcccga taaagatggg attatattgc ttgtcaagag tcaaatcata
      901 ggatacatca tccccaatat tgccagtgct ttttaccatg cgaaaaacgc cagcagagtc
      961 aaggcggcat ataaaaatgt ctgaccctcc tgaactaacc aggtcgaaag aatcccattg
     1021 agacgaaccg ctaaattttc ctgtaatgta tatctgtcct gagggagtat aaacaagtcc
     1081 ataggcaata tcttctccgc ttccaccggc acgttttgca aaaattgcgt ttcccgaagg
     1141 atttagtttt accacaaaaa catctttgct cccggcacat gtaagattta ctcctccaaa
     1201 tgttgccgta gctccaaaat atccagaaat gaaaatgttg ttttgttcat cggcatccag
     1261 gccaaagggt tgatcgtcgg caattccgcc ggagctttgc aatgaaagca gattgccata
     1321 gcggtcgtag cgagcaatga aaatatcatt atcgccggca ctagtcaccg aagaattacc
     1381 aaatgtggca ttaccttgga atatacctgt tacaaccact tcatcggcta tggtgtaaat
     1441 cacggcatgg cctctgtcgg gtgatagacc ccccccctgc tggatccagt ccaatactcc
     1501 ggcaccgttg tatcttgcaa taaaaacatc ttcaccgcca ctacttataa ggttgtggcc
     1561 ctgaaaatcg gcattaccct gaaaatatcc cgtaacgaaa caacttccgt cggaaacgac
     1621 atataaagcc aaagccttgt cttcaaaatt accccctcct gtttgtaccc attttaaaat
     1681 gcccgttgga ctatattttg ccaggaaagc atcactttcg ccttgtgcaa agtattggtt
     1741 gcctccaaaa agggccatca cgcggaaaga gcccgaaagg aaaatattac ctatactatc
     1801 taaacctata cctgtaactt cgtcatcgag gcttccacca atttgctgta tccattgtaa
     1861 atttcccaga tgattatatt tggctaaaaa aatatccttt ccgcctgtgc tggtaagcga
     1921 tgtgctccca aaaaccgcat ttccgccaaa agttcctgcc agaaatatat ttcccgcctc
     1981 atcggcaacg atgcagcgcc cctcatcatg ggctgctcca cctcctttac ggggtaaaaa
     2041 ccattgtgaa gtgtctttac caaaaattaa ggccggaggt tgagttatag tgaaacgttt
     2101 tatcagttta caattttcct catcgcgaac tgttacctga taagtatttg gaagcaatcc
     2161 agagatatgg gaagtattat ctccagaatt ccaggtaaat attaatggtt ctatgccacc
     2221 ggttgtcgta agatgaatag acccgtcggc ctctccaaag cacgaaacat tttttttagt
     2281 agccgtaaca ctcaaagtac aatgatcatc ctgccatttc cacaaatccc tgaaataccc
     2341 tgtggcatta tctcctccac acgaaatata ggcagcatca gaataagcaa aagatacgga
     2401 gttctcacgc ggtaatgctg gaaaatcgct tttacgtgac catagattgg cattcacatc
     2461 gtattcccat aagtcattca atagattggt gccgtcatct cctaaagcaa tatatccttt
     2521 ttgattgaga gcaaacccaa ctgctccact tcttccatgg cttaaagcgt ttatgggaga
     2581 ccatgtattg gatactctgt cataaacata aaaatccgaa agataagagc cattataacc
     2641 taagcccaca taagccttgg ttccaatgct aaaggcagat gcataaattc tgccttgtcc
     2701 tccaaaatta ttccttgcag tccaagaatt acttactgga tcgaattcgt aaaaatcctt
     2761 cctgtaagcg ccatcgtatc cgagccccac ataagccttg tcgccaatta cggtagagat
     2821 gcatccctga cgagccccac caggaaaatt tatacctgcc gaccatgtat gattcgttgt
     2881 gttatacacc caagtgtcca ataaaaatcc gccattgtat cctgttgtga tgtaaatttt
     2941 gtttcccaag gcaaaacaag cagcatatgc tctggcctgt cctggcatgt cggtaagctg
     3001 gatccatgaa tctatagcag gatcgtattc ccaaaaatct ttatggtaaa ttccgtcgaa
     3061 ccccatgcca atataagact tatcgccaat ggtacaagca acagctcctt ttctgccact
     3121 acctgcaaaa tcactctttc taatccaagt atcctggcct aagaccacgt tctgaagaaa
     3181 aagcatcaat aagaaacaga atgcaatggt aatcttgcta aaactattca tgggtcaatc
     3241 aattaaaaac ttggggttta ggttaacttt cattcttcga gagccctgaa caagattctc
     3301 tactttaaaa gcaactttgt cgagtagtag attataaatt tctaatagag taatttttat
     3361 ggcatttact gtcacattaa taggaatata ggattctaat gatgaaataa ttggttgaga
     3421 gttggtctta tggttctgtc tccatagctc gaaacttttt ccctttttgt aaatcgagtt
     3481 ttggcaaata atcgattggt ggtgaaatga tagagccctt tcaccacctc tggtaccaga
     3541 agaatgaaaa attaatggga agggcttcat catagctaaa tcattcatca tttataagct
     3601 aattgcacat cttttcgtat tagtaaacaa agttcaaatt tatgaataaa attttgcaat
     3661 gggctgaaac atcctcaaga tattttatat gaaagagaca atcagcgatc gattcttgtt
     3721 gtattgatgt catatttcag gagattcagc taaataggta caatgatcat cattgattca
     3781 ggggaaggta aaaattacgg tattcttcaa attgtgatgg gcgcatacct gagccatgaa
     3841 tgataattct ttgtctatcg agcaatccgt caggccggta aagacgttcg acttcaagca
     3901 tgatttctcc gttttcattg gtaacttgac gtgaaagttc tctgttgttc tcatcataat
     3961 tcacatgata tctcgagcgg ccaaatctgt tggtttccca atattcttct atataagggt
     4021 tgggattgta aattctttcc caagtttcga acagatttcc gttcttgcca tatacctttc
     4081 ggagaatgag gcggccttca tcatcataaa cagattcgct taaaatgtat tgatcctctg
     4141 tttcattttc aaattttact tcgcgtccaa gatcatcgta actccatttc ttagaactgg
     4201 tgaattgcac tgaatcatcg taagtatttt cctcgatcag cttatcatta ttatatcttt
     4261 tttcggtaat ctgttctctt tcgccgtcgt aattgataaa ttcaacttta tcaagcagat
     4321 tgtttgaatt gtaatgataa atttctctgt cgaatgattc atctacataa aaaatatctt
     4381 tttctatcaa tctcgacata gaatcatatt gaaatgttag tttttgagca aattcaccac
     4441 tggagtcaat ttcagattgt tcgatcaaat ttccagagtc gtcatactga aaagacatag
     4501 tagcctccaa attaccctcg ttatcatagc gtctttccat tactaatcgg ttgttttggt
     4561 cgtattcttg ctcaaggttc atgatgatgc caacgatatc atcctcagta aaattttccg
     4621 acttaaaaat gatacgcttc gtttcccttc tgtaatttcc tgtcatagct gattaaatga
     4681 tttaattggc cgtaaaaata gaaatttccg gcttaaagcg attcatatta ggtgcttcat
     4741 aaaaaagatg aataggtatg gggtcgagag taggagccat aacaggaatg attcgcaagg
     4801 atattgggtg tgtgggctaa ggaggtattg tttgcacata tattttttcc ctgcggattt
     4861 ttgttcacgg ttttattatt tgccatttgc agaaatagca agagaatcca aatgtttatc
     4921 tttgcctcac tttcattaac tattctttaa atatcttgtt atatgaagat agaattcaag
     4981 aaaatcatgc ctgtgttgtg tctgagcata gtggtaggtt tttcgtcctg cacacgaacc
     5041 gaaaaaatca cttatcccga aaccaaaaga gtagattcgg tggatgagta ttttggacaa
     5101 aaaattctcg atccttaccg ctggctcgag aatgacactt ctgccgaaac atccgaatgg
     5161 gtgaaggctc aaaatgaaat taccaatgct tatctcgaaa aaattccttt tcggaaatcc
     5221 ttgaaggaaa gatttacaca aatttgggat tatcctaaat atgggattcc cttcagcaag
     5281 gggaatcttt atttctattt caagaatgat ggaatgcaaa atcaaagtgt actctacgtt
     5341 caggaaggtc ttgaaggaga accgcgggtt ttactcgatc ccaatcgttt atccgaagat
     5401 ggaactgttg ccattgcttc attttcggtt tcgaacgacg ctcgattcct ggcttattcc
     5461 atagccaaag ccggttccga ctggaacgaa atttatgtga tggatataaa tacccgacag
     5521 aaccttcccg atcatattac ttggataaaa ttttcgggta tgtcgtggaa aggggatgga
     5581 ttttattata gccgctacga agcccctaag ccaggtgagg aactttcgat aaagaatcag
     5641 aatcagagag tattttatca caagttagga agtgatccta ccaaggatga attaatttgg
     5701 gaagaccccc aacatcctct gcgaaccttt ggtgccagcg ttaccgaaga tgaaagattc
     5761 cttattatct ctgaaagcga atcaacctat ggcaacaaat tacatattaa agacttgcaa
     5821 aaacccgctt catcttacat tcatatcggc gactctttcg actgggaatt ttgggtagtt
     5881 gacaatttaa atgatgactt gattgttgtc accaattatt tagctcctcg cagtcaggcc
     5941 gtgtccatca atttttcgca tcccgagatt tcttcgtggc aaacccttat ccccgaaaaa
     6001 gaagaagtcc tcgaaggaat ttatctggca aatggtaaga ttatttctca atatctgaag
     6061 gatgcttgta atcatttata ttcccataat ctcgatgggc agctgataga cgaaattccg
     6121 cttccaggca ttggtaccat cggaggtttt agttctgaaa aagagaaaaa tattgccttt
     6181 tttgctttca cctcatttac atatcccact accatcttta aatttgatgt agaaaaaaat
     6241 caaaccgagg tttttcgtaa acctgatatt aagtttaatt cagaggatta tactactgaa
     6301 caggtatttt atacaagtaa ggatgggaca aaagttccaa tgtttatagt gcataaaaag
     6361 aattttttga aaaatggtaa gaatcctacg ctcctatatg gctatggtgg gtttaatata
     6421 agcctcacac cttcatttag tatcagtttg attccttttc tcgaaaatgg tggagtttat
     6481 gcagaagcta acattagagg aggaggtgaa tacggagaag aatggcatca ggcgggtact
     6541 aagatgaaaa aacaaaatgt tttcgatgat ttcattgctg cggccgaata tcttatttcg
     6601 aataaataca catcgcccaa gaagctggct atcaatggag gtagtaatgg cggcctcctc
     6661 attggtgctg tgttgaatca gaggccagac ctgtttgcgg tggcagtacc cgaagtgggt
     6721 gtgatggata tgttgcgcta tcataaattt actataggat gggcttgggc ttatgattat
     6781 ggtacagccg aagatagtga ggaaatgttc cattatctgt tgagctattc gcctttgcat
     6841 aatatcaagc ttggtacgaa atatccagca gtcatggcaa tgactgccga tcatgatgat
     6901 cgggttgtac cggctcattc gtttaaatat accgccacgc ttcaggccgc acaagcaggt
     6961 gatgctccca tacttatccg tattgaatcg aaagccgggc atggggccgg aagacccacc
     7021 tccaagatta tcgacaatgt taccgataaa ttttctttca ttatgtataa ccttggaatg
     7081 agtcccaaat tttaagatag aattccatca tttaattgat tgttatataa ctgagaaacc
     7141 cgggttttaa ttcccgggtt tcttttgtaa acaagttaca attggtaatt ttgtacgatg
     7201 atatgtgccc tgctatgacg ataaaaccag gagataaagt acgatttctc agctcgagcg
     7261 gcggaggaat agtaacacga gtcattcggc ccggtatagt tgcagtagaa attgaagacg
     7321 gatttgagat accaacagct atcaacgatt tggttaaaat tgaagaaact tctcctgcag
     7381 ctcgattttt tgccgataat gacccctttc tttctcaaaa agacaatgaa tcaaaaaccg
     7441 aacccgccgt ctctccgccc ttcaagtcag agcaaaatgc ttctccttta aatgaaagtt
     7501 atatcgagaa actacccgca tcacgaaaat caaccatcaa tcctgaagga atataccttg
     7561 ctttcgagcc tcacgaccaa aatttattca tcattgggta tctcgattta tgtattgtaa
     7621 acttcagcag ttacgacgtt ttatatacct tttcccataa gagccccaaa ggaggttacg
     7681 taggaagaga ttatggttct atacctcctt attcgaaggt aatcatcgat atcattcagc
     7741 gggaagaat
//
LOCUS       DAOORU010000114         7703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_11716_length_7703_cov_5.396967, whole genome shotgun sequence.
ACCESSION   DAOORU010000114 DAOORU010000000
VERSION     DAOORU010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7703
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11716_length_7703_cov_5.396967"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PLF20_07605"
     CDS             <1..1186
                     /locus_tag="PLF20_07605"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOX80900.1"
                     /translation="PNLWEYNYRSMLNYMEQCLYGIRGIVTDSITSQPVKAMVFVKSH
                     DIDSSMVFTRLPWGNYHRLIAAGNWTLEFSAPGYVTKTISNVQTTNYNTVFLDVQLCP
                     IGVWADFAADKTQVEAGESVNFTDNSVGSPTQWVWNFDGGNPSTSNSQNPQNIFYEED
                     GVYDVALTASNSSYSNTKTKINYIRVGNQYLMDNQTITTCSGKFYDQGGPNYDYSADQ
                     HLITTFFPAEPGKKIRIAFESFALEASQDCTRDYLKIYDGSSTTSPIIGTWCGSEGPD
                     SVVSTDPSGALTVEFNSNMFVQTAGWVASIMCDSGVGISSQQKTKTFVHTSPTGKIEI
                     YNAKQGTVATLFSLSGSKLIEKGLNASTEIISTHSLPEGIYLLRLTSDTETKVYKILL
                     RK"
     gene            complement(1667..2254)
                     /gene="coaE"
                     /locus_tag="PLF20_07610"
     CDS             complement(1667..2254)
                     /gene="coaE"
                     /locus_tag="PLF20_07610"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOX80901.1"
                     /translation="MKKVALTGNMGSGKSLVTEIFQSMGIPVFQADVAGHEALNEPEI
                     IRQLRKRWGSQVLHPYTNQPNRKTIATIVFNDEDELLFLNELIHPIVRQYFENWYLKY
                     TDQPYVIHEAAILFESGFHTQFDKIIFVSAPSTLRIQRVMQRDGVEAEDIIARMNRQW
                     PEEMKIPLADYIIKNDEQQPLIPQVVRIHELLSQK"
     gene            complement(2256..3176)
                     /locus_tag="PLF20_07615"
     CDS             complement(2256..3176)
                     /locus_tag="PLF20_07615"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HOX80902.1"
                     /translation="MFFLLVSAVLWAIIRLSTEYEETLAIPLSFQTAVGDYSLVYYSD
                     TVIYAKAKLKGTQLASWQFRNNVNPFLLKFNNLRLKPNRQGYAELFIPTTQLSAELTT
                     WLHSTSSVVVIRPDTLYFGFERTYQKRVPVKPCLKYTLSPQYFLVDSVRVSPDSVLIQ
                     GPTRIIDTISFVNTHWMDAGEISSDKTFTVKIQNPYSHLGIKISDEKVNLKLKVERST
                     EATVELPIRLPDSASCSLKLFPDKVSLRLLVPFGVYKNLNTNDFLVSVACPDSSDFKR
                     KMLTVKVDRLPAGSKLVEIEPNEVEFLIFN"
     gene            complement(3333..3671)
                     /gene="yajC"
                     /locus_tag="PLF20_07620"
     CDS             complement(3333..3671)
                     /gene="yajC"
                     /locus_tag="PLF20_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOX80903.1"
                     /translation="MLFHFLSVLLVSQPQGKNAGSGLTALLPLIIIIVIFYFFFIRPQ
                     TKKAKEERKYRENLKKGDKVITIGGIHGKIVEVNDTTFIIEVEEGSKLKIEKTAVAMG
                     GSQTSVGSSK"
     gene            complement(3699..4190)
                     /locus_tag="PLF20_07625"
     CDS             complement(3699..4190)
                     /locus_tag="PLF20_07625"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOX80904.1"
                     /translation="MANKLENEAMKFFRFVLLIGSLLLIFSCTHRNENNLKGDLVKNP
                     VSLKKSDSTQSPVMKFETLEHDFGRVFAGEKVSYSFKFYNKGKSDLLITNVTASCGCT
                     VPEWSKTPVAPGEEGHITVTFNTAGRSGFQQKQITVLANTVPNTVSLMIKAQVYEPGS
                     NKE"
     gene            4270..4785
                     /locus_tag="PLF20_07630"
     CDS             4270..4785
                     /locus_tag="PLF20_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HOX80905.1"
                     /translation="MLIYLVGYMASGKSYTGSRLASKLGIRFIDLDHSIEEKYRISIK
                     NFFLKYGEDQFRIIENQMLHRTFQLADAVIATGGGTACYFDNMEQMNRHGLTIYLKQD
                     AEEVFQRLKRSKKPRPMLSASPDPELKEEILTQLRERVPYYEKAHIIFDIHKMNIHQL
                     ANEVLQYKNQF"
     gene            complement(4776..6185)
                     /gene="rlmD"
                     /locus_tag="PLF20_07635"
     CDS             complement(4776..6185)
                     /gene="rlmD"
                     /locus_tag="PLF20_07635"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOX80906.1"
                     /translation="MRNRNLPIIERLEITDAGAEGMAVGRFQDMVIFVPWAVPGDIVD
                     VRVVRKRKRFWEARLIRIIKESENRRKPWCSHFGICGGCRWQNMDYSTQLFYKAKWVN
                     DTLLRLGKFEFPEPLPILGSPGKVSYRNKMEFTFSNRRFLDEGENADDPEITLQALGL
                     HLPDRFDRVLDIQHCYLQPESSNKIRLAVKRFALEHGLVFQDLVTHHGFLRNLIIRNN
                     QEGDYMVIMVFGEYQPEPIHLLMDFLKETFTEIKSLYYLINTKANDSFSELEPILYAG
                     QEVIIEEMDDLKFRISPLSFFQTNILQTKCLYKVAMDFVELKAHEIVYDLYTGTGTIA
                     CFVAKHCQKVIGIEYVERAIEDARMNARMNSIENVEFLSGDVLQIMNQDFIAKYGFPD
                     VIITDPPRSGMHPQVVKRMLDILPSRIVYISCNPATQARDVSLLSEKYHVRKVQPIDM
                     FPHTSHVENVMLLERKDQN"
     gene            6354..7403
                     /gene="porQ"
                     /locus_tag="PLF20_07640"
     CDS             6354..7403
                     /gene="porQ"
                     /locus_tag="PLF20_07640"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HOX80907.1"
                     /translation="MRHRKNILVLSIILGLGNSFCQAQTGGTKTYEFLNLPTSARAAA
                     LGYNVLSISDNDISLVNTNPSLLNPEMHNNLVLSFVDYFADINYGYATYARSFSKTGN
                     FAATLQFIDYGTFTSADPTGSIQGSFGANELALNLSWGRSLDSNFSIGASLKGIYSSF
                     ESYTSTGIAVDVAGSFILPEQFLSISLIARNIGLQLSTYSSTGSEDLPLEIDLAVSKR
                     LEHVPLRFSIIATNLQKWDLTYTDPFLIETDPLTGEEKQKNGLSGFPDKLMRHFIFGA
                     ELQPVKALSLRLGYNYQRRQQLKLESKMGMVGFSWGIGLKISKFQLNFAHSIDHLAGA
                     PNYISLSTNFGDFLK"
BASE COUNT         2331 a         1603 c         1471 g         2298 t
ORIGIN      
        1 gcctaatctg tgggaatata attaccgttc catgctcaat tatatggaac aatgccttta
       61 tggaatcaga ggaatagtaa ccgattccat tacttcacaa cccgttaaag ctatggtatt
      121 tgtaaaaagc cacgacatcg acagttccat ggtctttacc cgcttgccat ggggaaatta
      181 tcaccggctc atagctgcgg ggaattggac actcgagttt tctgctccgg gttatgttac
      241 taaaactatc tctaatgtgc aaactacaaa ctataacact gtttttctcg atgttcaact
      301 ttgccccata ggcgtgtggg ctgattttgc agccgacaaa actcaggtag aggctggaga
      361 atcggtgaat tttaccgata attcggtggg cagcccaact caatgggttt ggaatttcga
      421 tggtggaaac ccatccactt ccaattctca aaatccccaa aatatttttt atgaggaaga
      481 cggagtttac gatgtagctc tgactgcctc taattcgtct tatagcaata caaagacgaa
      541 aattaactat atacgtgtag gcaatcagta tttaatggac aatcaaacca ttactacctg
      601 ttcgggaaag ttttatgacc agggcgggcc aaactacgat tattccgctg accagcactt
      661 aattactact ttctttcccg ccgaaccagg caagaaaata cgtattgcct tcgaatcatt
      721 tgctttggaa gcaagccagg attgtacaag ggattatttg aagatttatg atggttcatc
      781 gaccacttct ccaatcatag gaacctggtg cggttcggaa ggccccgatt cggtggtttc
      841 gaccgatcca agtggcgcac ttacggttga attcaattcc aatatgtttg ttcagacagc
      901 cggttgggta gcttcgataa tgtgcgattc aggtgtggga atatcttcac aacaaaaaac
      961 taaaactttc gtacatacct ccccaacggg aaaaatagaa atatataatg ccaaacaggg
     1021 cactgttgct acactttttt ccctctctgg aagtaaactc atcgagaagg gcttgaatgc
     1081 ctccaccgaa atcatttcaa ctcattcatt accagaaggg atataccttc tgcgccttac
     1141 ctcagacact gaaactaaag tttataaaat ccttctcagg aaataacttc caacgaaaaa
     1201 aagggaatca ataccaaaat tttatgcagc tgtgcccgaa ttttttactt gtttcctttc
     1261 tcgcagagaa ttcctaaatc ggcattaaat taaagtgtcg cagttttgaa gagggcgatt
     1321 gaatatcact tactctaaat gatcaatttc atcatgccgt tgaaaggtac ttctttcatc
     1381 tgaaacagct catttgttaa cgtttccatt tctgacgcta aatctaaaag cttcttggcg
     1441 tcatggcgag aaaagtattg cacgcaaagg ctgcagaatg ccctaaggag ttgaacagat
     1501 ttttttacat cacgaaaacc aattacattt aaacaaaact taataggttt taaagagagt
     1561 attttatgat aaattggtat gtaattacag gatcaaaagt tccgacttca atcatttttt
     1621 caaatttcga aactcgggtt aagtaagtca gcttgtagaa aaaattctat ttttgggata
     1681 ataactcgtg aattcgaacc acttgaggga ttaagggctg ttgttcgtca tttttgataa
     1741 tataatcggc gagaggaatt ttcatttcct cgggccattg tctattcatg cgagctataa
     1801 tatcctcagc ctcgactcca tctctttgca tcactctttg aattcgaaga gttgaggggg
     1861 cagatacaaa aatgatttta tcgaactgag tatgaaatcc actttcgaaa aggattgctg
     1921 cttcgtggat gacataaggc tgatcggtgt atttcaggta ccagttctcg aaatattgcc
     1981 gcacgattgg atggatcaat tcattgagaa ataaaagttc atcctcatca ttgaatacga
     2041 ttgtagcaat cgtctttcga tttggctgat tcgtataagg atgcaatact tgcgagcccc
     2101 agcgtttccg taattgccga ataatctcag gttcattaag agcttcatga ccagccacat
     2161 cagcctgaaa tacaggtatt cccatacttt ggaagatttc ggtaaccaaa ctcttcccac
     2221 ttcccatgtt accagttaga gctacctttt tcattttaat taaaaattag aaattccact
     2281 tcattgggtt caatttcaac taacttagaa cctgcaggca agcgatctac cttcactgtc
     2341 agcattttac gcttgaaatc ggaagaatca gggcaagcca cagaaactaa aaaatcattg
     2401 gtattcagat tcttataaac accaaacggt actaaaagtc gaagagaaac cttatcggga
     2461 aacaatttta aagagcaact cgccgaatcg ggcagtcgga tgggcaattc cacagtagct
     2521 tcggttgaac gctctacctt tagtttgagg ttgacctttt cgtccgaaat ttttattccc
     2581 agatgtgaat aaggattctg aatcttcaca gtaaaagtct tatcggaaga tatttcacct
     2641 gcatccatcc aatgtgtatt gacgaaagaa atggtatcga ttattctcgt gggtccttga
     2701 atcagaactg aatcaggcga tacacgaacc gaatcgacca aaaagtattg agggcttaat
     2761 gtatatttca ggcatggttt caccggaact cgtttttggt aagttctttc gaaaccgaaa
     2821 taaagtgtat caggtcgaat cacaaccaca gaagaggttg aatgaagcca tgtagtcaat
     2881 tcggccgaaa gttgagttgt tgggatgaat agctcagcat atccttgacg attgggtttt
     2941 aagcgtaaat tattaaattt cagtaagaaa ggatttacat tattacggaa ttgccaggaa
     3001 gccagttgag tacctttaag ttttgccttg gcataaatga cagtatcgga ataatatacg
     3061 agcgaataat cacccacagc cgtttgaaaa ctaagcggaa tggcaagggt ttcttcatat
     3121 tcggtcgaaa gcctgatgat tgcccataga acagctgata ctaacaaaaa aaacataaat
     3181 atgggcaagc cttttcgaaa tggcataatg cgctgccata atctgttaag aaagggtata
     3241 tgaattggca tgctcacgag aagaaattgt tcttccgaaa atacagcaaa tgcccctaat
     3301 cataaaatca caggcatttc aataaaaatt cattatttag aggaacctac actggtttga
     3361 gaaccaccca tggctacggc ggttttctcg atcttgagct tcgaaccttc ttccacttcg
     3421 atgataaacg tcgtatcatt cacttctacg attttaccat gaataccgcc aatagtgatg
     3481 actttatcac cttttttgag gttctcacgg tattttcgct cttcttttgc ctttttcgtt
     3541 tgtggacgaa taaagaagaa gtaaaaaatc acaatgataa taattagtgg aagcagggcc
     3601 gtaagtccgg aaccggcatt tttcccttgg ggttggctaa ccaataatac acttaagaaa
     3661 tggaaaagca taattgaaat gattaagtta ttgaaaattt attccttatt actgccaggc
     3721 tcatagactt gtgccttaat catcaaggaa acagtgttag ggacagtatt tgccagcacg
     3781 gtaatttgct tttgctggaa tccacttcgg ccggctgtat taaaggtaac agtaatatgg
     3841 ccttcctctc cgggcgcaac aggcgttttg gaccactcag gcacagtgca tccgcaactt
     3901 gcagtcacat tcgtaattaa aagatcagat ttgcccttgt tataaaactt aaaactatac
     3961 gaaactttct ctcccgcaaa tacacggcca aaatcgtgtt ccaaggtttc gaatttcatc
     4021 actggcgatt gagtagaatc gcttttcttc aaacttacag gattcttcac cagatcacct
     4081 tttagattat tttcgtttct atgggtacag gaaaaaatca acagcaaact tcctatcaat
     4141 aagacgaaac ggaaaaactt catagcttcg ttttctaatt tattggccac aaaattacaa
     4201 cttctctcgt aattttttca atcatcaaat gcatcgactt atttttgcac ctccttaatt
     4261 caccataaca tgcttatcta tctggtaggt tatatggcaa gcggtaagtc atacaccggc
     4321 tctcgacttg caagcaaatt gggcattcgg tttatcgatc tcgatcactc cattgaagaa
     4381 aaatacagaa tcagtataaa gaacttcttc ctcaaatatg gagaagacca atttcgaatc
     4441 atcgaaaatc aaatgcttca tcgaaccttt caacttgccg atgctgtaat tgctaccgga
     4501 ggtggaacgg cttgctattt cgataacatg gaacaaatga atcggcacgg acttacaata
     4561 tatttaaaac aagatgccga ggaagttttt caacggctga aacgatcgaa gaaaccccgt
     4621 cctatgcttt cggcttcccc cgatcccgag ttgaaggaag aaatattaac ccagctaaga
     4681 gaaagagttc cttattatga aaaagcccat attattttcg atattcataa aatgaacatt
     4741 catcaactgg ctaatgaagt tttacaatat aaaaatcagt tttgatcttt tctttcgaga
     4801 agcattacat tctcgacatg tgaagtgtgc ggaaacatat ctattggctg aacctttcta
     4861 acatgatatt tttccgataa caaacttaca tctcttgcct gtgtggctgg attgcaactg
     4921 atatatacaa tacgtgaagg caaaatatcg agcattcgtt ttactacttg cggatgcatc
     4981 ccactgcgcg gtggatccgt aataatcaca tcaggaaagc catatttcgc gataaaatcc
     5041 tgattcatta tctgaagcac atcgcctgat agaaattcta cattctctat actattcata
     5101 cgggcattca tgcgagcatc ttcaatggct ctttctacat attctattcc aatcactttt
     5161 tggcagtgtt ttgctacgaa acaagcaatc gtgccagtgc ctgtataaag atcataaaca
     5221 atttcatgag cttttaattc gacaaaatcc attgcaactt tgtaaagaca ttttgtttgt
     5281 agtatatttg tctgaaaaaa tgacagagga ctgattctga atttcaaatc gtccatttct
     5341 tcgataataa cttcctgacc tgcatataaa atcggctcga gttctgaaaa agaatcattt
     5401 gcctttgtat tgatgaggta gtagagcgat ttgatttcgg taaaagtttc ttttaaaaaa
     5461 tccattaaaa gatgaatagg ttcaggttga tattctccaa agaccattat taccatgtaa
     5521 tcgccttctt gattattacg aatgatgagg ttgcgtaaaa atccgtggtg ggtaaccaga
     5581 tcctgaaaaa ccaaaccatg ttcgagggca aacctcttca cggccaaacg aatcttattc
     5641 gaggattcag gctgaagata gcaatgctgg atatcgagta cacggtcgaa ccgatcaggt
     5701 aaatgcaaac ccaaagcctg cagggtaatt tccggatcat ctgcattttc gccctcatcc
     5761 aagaaccttc gattactgaa ggtaaactcc attttattac gataagatac ttttcctggt
     5821 gaaccgagaa tgggaagagg ttctggaaat tcaaatttac ctaaacgcaa caaggtgtca
     5881 ttcacccatt tggccttata gaacaattga gtagaatagt ccatattttg ccagcggcat
     5941 cccccacata taccaaaatg gctgcaccaa ggttttctcc tgttttcgga ttccttgata
     6001 attcttataa gacgagcttc ccagaatcgt tttcttttcc ttactacccg aacatcgaca
     6061 atatcgccag gtacagccca tggaacaaat attaccatat cctgaaacct cccaactgcc
     6121 ataccttcag cacctgcatc ggttatctca agccgttcga taatgggaag attcctattg
     6181 cgcattgcta caaaacttcg gcttacaaaa ttactttaaa ttcaacaacg ggctccatgt
     6241 aataaaaacc actctcctcc gtttaggtga aatcagacac ggcaattggt tgtcatactg
     6301 cttttgttta cttttacccg ccgttttata atattaaatt taatcctgac acagtgagac
     6361 atagaaaaaa tatccttgta ttgagcataa ttttaggctt ggggaattct ttctgccagg
     6421 cgcaaacagg aggcacaaaa acttacgaat tcttgaattt acccacatcg gcgcgtgctg
     6481 ccgccttagg ctataatgta ttgagtatca gcgataacga catttctctg gtcaatacca
     6541 acccctcact tttgaatcca gaaatgcaca ataatttggt tttgagcttt gtggattatt
     6601 ttgcagatat caattatggg tatgccacct atgcccgttc attctccaaa accggaaact
     6661 ttgcagctac cctacaattt attgattatg gcacctttac ctccgccgac cctacgggta
     6721 gcattcaagg aagttttgga gcaaatgagc tggctttgaa cttaagttgg ggtcgaagtt
     6781 tggatagcaa ttttagtatt ggagcaagtt taaagggaat ttattcctcc ttcgagtcat
     6841 atacctctac tggaatagcc gtagatgtgg ccggttcgtt tattcttccg gaacaatttc
     6901 tcagcatttc actcattgct cgtaatatag gattgcaatt atcgacttat agctcgacgg
     6961 gcagcgaaga ccttcctttg gagatcgatc ttgccgtaag taaacgcctc gagcatgttc
     7021 cactgcgttt ctcgataata gccaccaact tgcaaaaatg ggatcttact tatactgacc
     7081 catttctcat cgagaccgat ccgcttaccg gagaagaaaa acaaaaaaac gggcttagtg
     7141 gcttccccga caaacttatg cgtcatttca tttttggggc agaactccaa cccgtaaagg
     7201 ccttaagcct tcgcctgggt tataattatc aacgcaggca acaattaaaa ctagaatcaa
     7261 aaatgggtat ggttggtttt tcatggggca taggtctgaa aatttccaag tttcaactca
     7321 attttgctca ctccatcgat catcttgccg gcgctcccaa ctacatttcc ctatctacca
     7381 acttcggcga tttcttgaaa tgaaattgtc aatacctaaa tgacgttgac cgtttttaac
     7441 ctgattttta aaattgattc aagagatatc taaactctgc tctgtgaaat tctgcgtata
     7501 acccggcaca tctttatgtt ctctgtagtt aatttttcct aaccacagag agcacggagg
     7561 agcttagagg gtgttaatct tttttcgctg agtatctcgg tgtactctgt ggttaaaaag
     7621 gaggaaagga aaacggagaa agggagacga ggggacgaga agttagggaa ttggaatttg
     7681 gaatttgtaa tttggagttt gaa
//
LOCUS       DAOORU010000115         7689 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_11733_length_7689_cov_4.875950, whole genome shotgun sequence.
ACCESSION   DAOORU010000115 DAOORU010000000
VERSION     DAOORU010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7689
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11733_length_7689_cov_4.875950"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..890
                     /gene="mazG"
                     /locus_tag="PLF20_07645"
     CDS             90..890
                     /gene="mazG"
                     /locus_tag="PLF20_07645"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOX80908.1"
                     /translation="MNSENQQALEKFEQLLEIMDKLRAECPWDRSQTSESLRHLTIEE
                     TYELSDAILSHDGDAIRGELGDLMMHLVFYAKIASERGDFNLAEVLEGINRKLINRHP
                     HIFGDAVVTSAQEVADNWEKIKLEKEGKNSVLEGVPPSLPPLMKAYRMQEKARGIGFD
                     WENSQQVWEKVLEEMQELKQEVENLSPKERLEEEFGDLLFSLVNYARFIGVNPEDALE
                     NTNRKFISRFQYMEDVLNFNGQKFDQLTLNQMDKLWEEAKKSEKNQGK"
     gene            complement(980..1795)
                     /locus_tag="PLF20_07650"
     CDS             complement(980..1795)
                     /locus_tag="PLF20_07650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02757 family protein"
                     /protein_id="HOX80909.1"
                     /translation="MEKGKYFLDLKFFLDEAAEFFNCPEFIPEDPICIPHRYQMREDI
                     EISAFLAAIMAWGNRKTIITKASELLERMHNHPFEFIMDDNPKKYKFVEGFKHRTLND
                     EDIIFLLEGLHFIYKKHQSPEKLFMPRQNEPDTSGGIQRFVELMLQSPHSKRSLKHLP
                     NIEKGSAAKRINMFLRWMVRKDDKGVDFGLWYIPANKLLCPLDVHSGRTARELGLLSR
                     PQNDLKAVIELTSHLRELDANDPIKYDFALFGLSMQKTSLWTSISGRPGDEEG"
     gene            complement(1795..2928)
                     /locus_tag="PLF20_07655"
     CDS             complement(1795..2928)
                     /locus_tag="PLF20_07655"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOX80910.1"
                     /translation="MTRKKKSKKHKLVGGFLRLISLLIAIGLIISCIAQFVPPDKFWP
                     PAFFGLSFLIWFVANLFFLIFWIIRRARFAWIHVACLILSLHLFSHFIPLKGSKSSDK
                     LPASISLLSYNIKGLAGVNSIKSSGLSDSLFNFIKNENANIVCLQEYTERRRENKPPA
                     ELVFKSMDLDHFAKSAYFKNKEVARLFIATFSQFPICNKIEVHSDHRLIALITDIVVE
                     KDTFRVFNVHLQSISFNDENYRFLMEAQQNISSLQSDSMQQDSKKIFWKIRTGFIKRA
                     GQARLLREIIETSPYPVVVCGDFNDTPASYAYHTIKRGLTDAFMEKGKGIGNTYFGNL
                     PKIRIDYVLLHPKFQCQRFEIIKQTISDHHPVKAVFYKKKYDE"
     gene            complement(2992..3894)
                     /locus_tag="PLF20_07660"
     CDS             complement(2992..3894)
                     /locus_tag="PLF20_07660"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOX80911.1"
                     /translation="MQGYSFHHQVTSPWKQFTGFFRRGDALSVLILINVVIWVIISLI
                     RVGFALSGESSFAFLQSTLAFLGAPASPEALATSFWTLITYSFVHIDFFHLLFNMLWL
                     YWIGKLFIEYMSQHQLIWVYLLGGMSGYLVYMLAFNAFPGFLHIAPTSRLIGASAAVM
                     AIVTAIAFYLPNYRINLLFLGRIRLIHLAIALFVLDFLMISSNNPGGHLSHIGGCIFG
                     YLYVLSIRKNWLKKLKLKVLFKPKPKAKIKYRPGSEKEEKEYFEDKKHMEEEIDRILD
                     KIKLTGYQGLTAKEKETLFKYSKK"
     gene            complement(4014..4850)
                     /locus_tag="PLF20_07665"
     CDS             complement(4014..4850)
                     /locus_tag="PLF20_07665"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOX80912.1"
                     /translation="MANDEHRLTGFLGLTPVVKNLVLINILFFFATFALLGSSRGQLD
                     LTEYLALYYPGSDKFRWWQLVSYMFMHANFSHIFFNMFALWMFGNILEQLWGPKRFLT
                     YYMITGIGAAIVHIAVMYYQFHILDGQIKEFMLSPNPVDYANLIAKNFKGIYNPEWFD
                     QLISAWSLTPESPLLKQEALNSLGHLVVTKMNIPTIGASGAVFGILLAYGLTFPNSLI
                     YIYFLLPIKAKWFVLIYAVLEFWFGISGAGDNVAHFAHLGGMIFGYLLMVYWHKRDHR
                     YY"
     gene            complement(4937..6763)
                     /gene="mutL"
                     /locus_tag="PLF20_07670"
     CDS             complement(4937..6763)
                     /gene="mutL"
                     /locus_tag="PLF20_07670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOX80913.1"
                     /translation="MPDLIQLLPESVSNQIAAGEVIQRPSSAVKELLENAIDAGADSI
                     QLIVKDAGKTLIQVVDDGCGMSPTDARLCFERHATSKIHQAQDLFAIRSLGFRGEALA
                     SIAAIAQVELRTRRHEDEIGTQVLIEGTEVIDQQPIQCQAGSTFLVKNLFFNVPARRA
                     FLKSEAVEMRHIIEEFERVALVYPEIQFSLVHNGRTVFQLLKSTQKQRIVQIMGNHLN
                     PLIVPVEEQTDFIKIHGFTTKPEYSKKIRGEQYLFTNGRYVKIPYLQHAIESAYQGLI
                     PQGSFPSYFIYIQIDPSNIDVNIHPTKTEIKFINENLIYAVLKAATRKSIGQFSISGT
                     INFDEEWAFGDGELKPGQTIKPPKIHVNPNYNPFQHQNSQPSHERRNLENWQKLYEFP
                     EKEQRKNKDEYSAQLSSPPSQESVAIKKENERGFLQVHKKYLMTVVKSGVMLIDIEKA
                     KQRIFYENFLQALEKEGIPSQQEIFPLNIHLGQAESLLVNQMKPTLALMGFTIDNLST
                     NTFVIQGIPQGMDASEARESLETIIDSLLREEDIEHADIRVISAKTMAATLARHQLRP
                     MNEEEMRTLTDQLFSTAMPSLSPFGEKILNIIPLESIEELLK"
     gene            complement(6763..7014)
                     /locus_tag="PLF20_07675"
     CDS             complement(6763..7014)
                     /locus_tag="PLF20_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80914.1"
                     /translation="MAFLYRPKPKKYDHKPIYYNPDEEKAKRWQEKKESSDPTSDKLR
                     RDIERQWALRQKKKKSGNISLIVYILLILFLLYIIFILG"
     gene            7156..7530
                     /locus_tag="PLF20_07680"
     CDS             7156..7530
                     /locus_tag="PLF20_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HOX80915.1"
                     /translation="MKPEKLKNIILIGDRVLIKAKSANSKTKGGLVLPPGYSEREEVQ
                     SGYVIKTGPGYPIPLPSESDIEPWKSKDEAPSYIPLQAKPGDLALFLQKGAIEIVIDS
                     EKYFIVSHQSLLLLERDEELFT"
BASE COUNT         2311 a         1427 c         1625 g         2326 t
ORIGIN      
        1 aaaacttaat cttcatctcc tctgaaaaag ttgcatttat tagttgaatt tagttcagaa
       61 taaaaataga atttgtaaaa taaattgaaa tgaattctga aaatcaacaa gcgcttgaaa
      121 aatttgaaca acttctcgaa atcatggata aacttagggc agaatgtcca tgggatagaa
      181 gtcaaacgag tgaaagcctg aggcatttaa ctatagaaga aacctacgaa ctttccgatg
      241 ctatactcag ccacgatgga gatgcgattc gtggtgaatt gggcgatttg atgatgcatt
      301 tggtattcta tgcaaagata gcctccgaaa gaggtgattt taatttagct gaggtgttgg
      361 aaggcataaa ccggaagctc atcaaccgac atcctcatat ttttggagat gcggtagtta
      421 ccagtgctca ggaagttgcc gataactggg aaaaaatcaa actcgaaaaa gaagggaaaa
      481 attcagtatt ggagggagtg ccaccttctc ttccgccttt gatgaaggct tatcgtatgc
      541 aagaaaaagc tcgtggcatt ggtttcgatt gggaaaattc gcaacaggtt tgggaaaaag
      601 tgctcgagga gatgcaagaa ttaaaacaag aagtcgaaaa tttatcccct aaagaaagac
      661 tcgaggaaga gtttggcgac ttgttgtttt ctttggtcaa ttatgcacgc tttataggag
      721 tgaatcccga agatgccctc gaaaatacca atcgcaaatt catcagccgt tttcaataca
      781 tggaggatgt gctaaacttc aatggtcaaa aattcgacca gctcacattg aatcaaatgg
      841 ataaactttg ggaagaagca aaaaaatcgg agaaaaacca gggtaagtga attctgaaat
      901 ggagaatggt gagatgaaag ttaaaaatga gttcttatgg gttttatggt gaagttaaag
      961 tctctcagtc tctccgtctt caaccttctt cgtctcccgg ccttcccgat atgctcgtcc
     1021 aaagggatgt tttttgcata cttagcccaa acagtgcaaa atcgtatttt atcggatcgt
     1081 tagcatctaa ttccctcagg tgggaagtta gttctattac tgctttcaag tcgttctggg
     1141 gtcgggatag caggcctaat tcacgtgcgg tgcggccgct atgcacatcg agcgggcaca
     1201 ataatttatt cgccggaata taccacaggc caaaatcaac acctttgtcg tcctttctaa
     1261 ccatccatct taaaaacatg ttgattctct ttgcagccga gcccttttcg atgtttggta
     1321 aatgtttgag agagcgctta gaatgtgggc tttgcagcat cagctccaca aatcgctgaa
     1381 taccacctga tgtgtcgggt tcattttgcc gaggcatgaa aagtttttca ggactttgat
     1441 gtttcttata gatgaaatga agcccttcta acaaaaagat tatatcttcg tcattcaggg
     1501 tcctgtgttt gaagccttcg acaaatttat attttttggg attgtcgtcc ataatgaatt
     1561 caaagggatg attgtgcatg cgctcgagca gttctgacgc ttttgtgatg atggtttttc
     1621 gatttcccca agccatgatg gcagctaaaa atgcacttat ctctatatct tctcgcattt
     1681 ggtaacgatg tggaatgcaa attgggtctt ctggaatgaa ttctgggcaa ttgaaaaatt
     1741 cagcagcctc atctaaaaaa aatttcaaat caagaaaata tttccccttt tccattattc
     1801 atcgtatttc ttcttgtaaa agactgcttt tacaggatga tggtccgaaa tggtttgttt
     1861 gatgatttcg aatctctggc attgaaattt gggatgtagc agcacataat caattcgaat
     1921 tttaggaaga tttccaaaat aggtgttgcc aatgcctttt cctttttcca taaaggcatc
     1981 tgtgagtcct ctttttatag tatggtaggc ataagatgcc ggagtgtcgt tgaaatcgcc
     2041 gcaaaccacc acaggatatg gtgaagtttc gattatttca cgtaagagcc gggcctgacc
     2101 agcgcgtttg ataaatccag tccgaatctt ccagaatatt ttcttggaat cttgctgcat
     2161 ggaatcgctc tgaagtgaag aaatattttg ttgagcttcc attaaaaacc tgtaattctc
     2221 atcgttaaac gaaatagact gtaaatgaac attaaataca cggaaagtat ctttttcgac
     2281 cacaatatca gtaattaacg ctatcaatcg atggtcactg tggacttcta ttttgttgca
     2341 aatcgggaat tgactaaagg tagctataaa caaacgggca acttctttat ttttaaaata
     2401 ggccgatttg gcgaaatgat ccaaatccat ggatttgaaa accagttccg ctggaggctt
     2461 gttttccctt cgcctttcgg tatattcctg caaacaaaca atgttggcat tttcattttt
     2521 aataaaattg aacaaagagt cggataagcc tgaactttta attgagttta cacctgcaag
     2581 tcctttgatg ttataactga gcaagctaat agaagcaggc agcttgtcgg agcttttgga
     2641 acccttcaat gggatgaaat gcgagaagag atgaagagaa agaataaggc atgctacatg
     2701 tatccatgca aaacgagccc tgcgaatgat ccaaaaaata aggaagaaca ggttagccac
     2761 aaaccagatg aggaatgaaa gtccgaagaa tgcaggaggc cagaatttat cgggaggaac
     2821 gaactgagct atacagctta taatcaatcc aatagcaatc aataggctaa tcaaacgaag
     2881 gaagccccct accagtttgt gcttcttgct tttctttttc ctcgtcatta ttcttatcgt
     2941 gttttaataa tttggaagaa tctatgaatt tgtatgatag aatctacgtt tttacttttt
     3001 actgtatttg aacaaggttt ctttttcttt agcagttagt ccttgatatc cggttaattt
     3061 aatcttatcg agaatacgat cgatttcttc ttccatgtgt tttttatctt cgaaatactc
     3121 tttttcttcc ttttcgcttc ctggcctgta tttaattttc gcttttggtt tgggtttgaa
     3181 caatactttc agttttagtt ttttcagcca gtttttacga atggataaaa catagaggta
     3241 gccaaaaata catcctccaa tatgtgatag atgtccgccc gggttattcg agctaatcat
     3301 caggaaatcg agaacaaaca gggcaattgc aagatgaatc aggcgaatgc gacctaagaa
     3361 cagaagattg attctgtaat tgggcaagta gaatgcaatg gcagtaacta tggccatcac
     3421 ggcagccgaa gcacctatga gtcttgaagt aggagcaata tgcagaaatc cggggaatgc
     3481 attgaaggct agcatatata ccagatatcc cgacatcccg cctagaagat atacccaaat
     3541 caattgatgc tgcgacatat attcgatgaa tagttttcca atccaataca accacagcat
     3601 attgaaaagc aaatggaaaa agtcgatatg aacgaaactg tatgtaatca aagtccagaa
     3661 ggaagtggca agtgcttcag ggcttgcagg agctcctaag aatgccagtg tgctttgcag
     3721 aaaggcaaat gaagattcgc ccgagagagc aaaccccacg cgtatcaaac tgataatcac
     3781 ccaaatcaca acgttgataa gaatcaatac agacagggca tcgccacgac ggaaaaagcc
     3841 agtaaactgt ttccatgggg atgtaacttg gtgatgaaat gaatatcctt gcataaagcg
     3901 aatttttcga ttccaaattt agcagtgtgc tattacactt gccagaaata taaactattt
     3961 aacctcgaaa tttatttctt gaaataaaaa aattcatgaa tagcgaatca tggtcagtaa
     4021 tagcgatggt ctcgtttatg ccagtacacc atgagaagat aaccgaaaat cataccgccc
     4081 agatgggcaa aatgggccac attatcgcct gctccgctga ttccaaacca aaattcgaga
     4141 actgcataga tcaaaacgaa ccattttgct tttataggaa gaaggaaata aatataaatc
     4201 agcgaattgg ggaaggtgag gccataagcc agcaaaatac cgaagacggc tccgctggcg
     4261 cctatagtag gaatattcat cttggtaact accagatgac ccaacgaatt gagtgcttct
     4321 tgcttcaaca gaggactttc gggagtaagg ctccatgcac taatcagctg gtcgaaccat
     4381 tcgggattgt aaattccttt aaagtttttc gcaatcaaat tggcataatc gaccggattg
     4441 gggctcaaca tgaattcctt gatttggcca tcgagaatat gaaactgata atacatcact
     4501 gcaatatgaa ctatggctgc accaatgcca gttatcatgt aataggtaag aaatcttttt
     4561 ggtccccaca attgttctaa tatatttcca aacatccata aggcaaacat attgaaaaag
     4621 atgtgcgaga aattggcatg catgaacata taagaaacca gttgccacca tctgaattta
     4681 tccgaaccag gataatagag tgcaaggtat tcggtaaggt cgagctgccc tcgtgatgaa
     4741 ccaagaagag cgaaagtggc aaagaagaat aatatgttga taagtactaa attcttaaca
     4801 acgggtgtta atcctaaaaa acctgtcaat cggtgttcat cgttggccat gagtgttatg
     4861 aattagagga tatattggga tgaataatcg gcaaatatga tgaaacttta gtaaataaca
     4921 ataggggagt ataaagttat ttaaggagtt cttcgataga ttccaaagga atgatattta
     4981 gaattttctc tccaaaaggg cttagtgagg gcatggcagt ggagaaaagc tggtcggtaa
     5041 gtgtgcgcat ttcttcttcg ttcattggtc gaagttgatg ccgggctaag gttgctgcca
     5101 tggtcttggc cgaaataact ctaatatcgg catgttcgat gtcttcttct ctcaataaac
     5161 tgtcgataat tgtttcgagt gattctctgg cttcggaggc atccattcct tgaggtatgc
     5221 cctgaatgac gaaagtattg gtactcagat tatctatggt aaaacccatc agcgcaagtg
     5281 tgggtttcat ctgattcact agaagagatt cggcttggcc aagatgaatg ttcaaaggga
     5341 aaatttcctg ttgggatgga attccttcct tttcgagagc ctgcagaaaa ttctcataaa
     5401 aaattcgttg ttttgctttt tcgatatcaa ttagcatcac acccgatttt acaaccgtca
     5461 tcaaatattt tttatgaacc tgaagaaaac ctctttcgtt ttcttttttt atcgcaaccg
     5521 attcctgaga tggtggggaa gataactgtg cagaatattc atctttattt tttctttgtt
     5581 ctttttcggg aaactcgtat agtttctgcc agttttcgag attgcgtctt tcgtgggatg
     5641 gctgcgaatt ctgatgttga aagggattgt aattgggatt aacgtgaatt tttggcggtt
     5701 tgatggtttg gccgggtttt agttctccat cgccaaaagc ccattcttcg tcaaaattga
     5761 tggttcccga aattgaaaat tgcccgatcg attttcttgt tgctgccttt aatacggcat
     5821 atatcagatt ttcgttgatg aatttgattt ctgttttggt gggatgtata ttcacatcga
     5881 tattggaggg gtcgatttga atatagatga aataagaagg gaaagagcct tggggaatga
     5941 ggccttggta agccgattct atggcatgtt gcaaataggg gatttttaca tatctaccgt
     6001 tagtgaataa atactgttcg ccccttatct ttttcgaata ttctggtttt gtcgtaaatc
     6061 cgtgaatttt aatgaagtcg gtttgttctt caacggggac gatcagggga ttgagatgat
     6121 tgcccataat ttggacaatt ctttgttttt gtgtagattt taaaagttga aataccgtac
     6181 gtccattatg aacaagtgaa aattgaattt cgggatatac gagtgccact ctttcgaatt
     6241 cttcgatgat atgcctcatt tcgacggctt ccgactttag gaaagctcgc cgggcaggaa
     6301 cattgaagaa gagattcttc accaagaatg tcgaacctgc ctgacattgg atgggctgct
     6361 gatcgatcac ctctgtgcct tcgatcagca cctgagttcc tatttcgtct tcgtgacgtc
     6421 gtgtgcgaag ttcaacctga gcaatggctg caattgatgc caaggcttcg cctctgaaac
     6481 ccaatgagcg aatggcaaat aaatcttgag cctgatgtat ttttgaggtc gcatggcgct
     6541 cgaaacatag tcgggcatcc gttgggctca taccacaacc atcatctacc acttgtatca
     6601 gggttttacc ggcatctttt acaatcaatt gaatagagtc ggcgccggca tctattgcat
     6661 tttcgagtaa ttctttcact gctgatgaag gcctctgaat gacttcaccg gcagcaatct
     6721 gattggatac agattcagga agaagttgta tgagatctgg cattaaccaa ggataaaaat
     6781 gatatataat aaaaacagaa taagtaatat atatacaatc aaggaaatat tgcccgattt
     6841 tttcttcttt tgtcttaaag cccattgcct ttctatatcc cggcgcaatt tatcagaagt
     6901 aggatcggaa gactcttttt tctcctgcca tctcttcgcc ttttcttcat caggattgta
     6961 ataaataggt ttatgatcgt atttcttggg tttgggacga tacagaaaag ccatattgtt
     7021 acggaatgat tgaaatttgt tatcgatttt gcaaaattac ttcatttgcg tttatttggg
     7081 ttcgaattaa acattaaaaa cgaacttagg gaggaaatag taattttgcc ataaaattca
     7141 tttcaagcaa attcaatgaa gcctgaaaaa ttaaaaaaca ttattctaat tggcgatagg
     7201 gtgttgataa aagccaagag tgcaaatagc aaaactaaag gtggtcttgt tcttcctccg
     7261 ggttattctg aaagggaaga agtgcaatcg ggttatgtga ttaaaaccgg gccaggttat
     7321 cctattcctc tacccagcga aagtgatata gaaccatgga aatcgaaaga tgaagctcca
     7381 tcttatattc cacttcaggc taaacccggc gatttagccc tgttccttca aaagggtgct
     7441 atcgaaatcg taatcgacag cgaaaaatat tttatcgttt ctcatcaatc cttgttactg
     7501 cttgaacgcg atgaagagct ttttacttga aaaccatcgc attattgctt taattgcctt
     7561 agcttcatca tggtttctaa tttttccttc ctgccaaaga aacattcagc aggatgaaga
     7621 ttcgggtttt cgactcgata ccattgcttc gccccaatat gctggattgt ttgttgctgg
     7681 ttatattca
//
LOCUS       DAOORU010000116         7664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_11775_length_7664_cov_4.637009, whole genome shotgun sequence.
ACCESSION   DAOORU010000116 DAOORU010000000
VERSION     DAOORU010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11775_length_7664_cov_4.637009"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1411)
                     /locus_tag="PLF20_07685"
     CDS             complement(170..1411)
                     /locus_tag="PLF20_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOX80916.1"
                     /translation="MKLPISISLRYLLGKKTHHAINFVSGISVAGILTGTAALVIILS
                     VFNGFQQVIQSLYNVFDPDIKITAVKGKVFKPNEDIKNRISSMAGVAGISETIEDNAL
                     FRYRNHQHIGLIKGVDQTYPLFSHLREKILQGEFVLSDSTGPYAVVGAGVAYYLNMGL
                     DDAFYPLEVFVPSRKSNLSALFSEEAFNSLAIRPSALFSVQQDFDISYVITPLSFARK
                     LLEYSTEVSAFEVYLKPNADIKSIQKKISEMMGSGFKVANRYEQQASLYKIMRTEKYA
                     IFIILAFILIVATFTLIGSLSMVISEKQRDIAIFMAMGAPTSYIRKVFLYQGLWISVA
                     GGIGGTLLGAFICFLQQTFGFVKINTSGSSFVISQYPVEMQGMDFLVVFVFVVIIGII
                     ASWIPARKSTQKSFIAYLRVE"
     gene            complement(1425..2252)
                     /locus_tag="PLF20_07690"
     CDS             complement(1425..2252)
                     /locus_tag="PLF20_07690"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOX80917.1"
                     /translation="MQYDPIKSDLGRFFNRTPRLRILFYRLLNLLLLRTWHVKKELRI
                     WESQHRETAKILDAGMGFGQYSYLLAQHHPNWEILGVDIKDDQVDDCNTFFRKAGLKN
                     ARFEVADLERFSSPPLFDLILSIDVMEHIPNDEAVFQNFFHALKPGGMLLISTPSDQG
                     GSDVHPHDEDISENASHSFIGEHVRDGYNILMIQKKLREAGFSCTEAYYTYGKPGKIS
                     WNLSMKYPIVMLNLSKLFFILLPFYYLIAFPFALLLNQMDISRKHSSGTGLIVKAWK"
     gene            complement(2258..2593)
                     /gene="rbfA"
                     /locus_tag="PLF20_07695"
     CDS             complement(2258..2593)
                     /gene="rbfA"
                     /locus_tag="PLF20_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOX80918.1"
                     /translation="MEKTRLEKVARLIQKEMSLMLQDAVPYTGGGILTVTRVSVTPDL
                     QIARIHISIFGLPDKKAALEQLKLHGPELRYQLGVKIRHQVRIIPQLQFFLDDSLDYI
                     ERIDKLLNQ"
     gene            complement(2829..3050)
                     /locus_tag="PLF20_07700"
     CDS             complement(2829..3050)
                     /locus_tag="PLF20_07700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80919.1"
                     /translation="MYPNPVKDYQIIESGPQISEAILLYNLSGKLVLRSWLQTQTQRL
                     STSFLPSELYLWNIQYHGKTIEQGKWTKE"
     gene            complement(3774..4331)
                     /gene="ruvC"
                     /locus_tag="PLF20_07705"
     CDS             complement(3774..4331)
                     /gene="ruvC"
                     /locus_tag="PLF20_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006844542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOX80920.1"
                     /translation="MTGQDRIILGIDPGTTIMGYGLIECQKGEIELLTLGVLRLSRIA
                     NQPLKLKRIFERTLSLIDEYKPDELAIEAPFYGKNVQSMLKLGRAQGVAIAAALYRNI
                     PIFEYSPKKIKQSVTGNGNASKQQVAAMLGRLITIAETPESLDATDALAAAVCHYFQD
                     DPHSEVKKYTGWKAFLNDHPDRRED"
     gene            4349..5374
                     /locus_tag="PLF20_07710"
     CDS             4349..5374
                     /locus_tag="PLF20_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HOX80921.1"
                     /translation="MTKVTKRNINIFLQILILIGAYGYLAWKLFIAPSTRLTAQNISQ
                     LFHISNFWLVISIVLLLMLVNWSIEAAKWKYLISKIEEVSFPLALKAIFTGVTVSIFT
                     PNRVGEFFGRVFLLKNRQPMRGILITILGSFSQIVIYMICGITGGIIMFYLFLLPQNP
                     SLTKLQWIIISLAVIILAVTILIFLNISFLGSILQNLIPKQWHRIKRWFAILSYYSKR
                     ELTKVLIMSFLRYCVFSFQYIILLRMFNVPLSVGNLLVALMVIFALLVIIPSVALSEL
                     GVRSTVAVFVIGMMLPVGLRNDPMVNLGIIGAASTLWLINVALPAIIGSFFVFKLKFF
                     KGNGPKS"
     gene            5381..6481
                     /locus_tag="PLF20_07715"
     CDS             5381..6481
                     /locus_tag="PLF20_07715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX80922.1"
                     /translation="MDKLSFLILIIPCIYALMMIAFAFGWLWLPAYRNECRKSVSIII
                     AVRNEEENIPQLLQGLHLQSYPTHLLQIIIVDDHSTDRTFQLIENDPLVQNIRLPSNL
                     QGKKAAIQKGIEEAKGEILLFTDADCFLPPDWVTSMIMPFANEKIHLVAGPVQIKGEG
                     LWAKIQEIEFYSLMAVTAGSIGIRLPLMNNASNMAVRKQSWYEAVLMGLGKKFVSGDD
                     VFMMMSIAKIFGSRSITFLKSNKAIVVTKALRNISDFFRQRMRWASKTKGYSAILPRL
                     VAFIVLLTNLLFLISMFWAIFYFPAFSLTIEIFIVKSLADLVVMIPALRFFQSLWKIP
                     AIIFLQPFYPFYVVSVALMSLWARNVWKGRRI"
     gene            6594..7292
                     /gene="tsaB"
                     /locus_tag="PLF20_07720"
     CDS             6594..7292
                     /gene="tsaB"
                     /locus_tag="PLF20_07720"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOX80923.1"
                     /translation="MTNLLCIESSTSICSVALANEGQLMGLEETEDKNAHSARLTVLI
                     EKLLKDNNLKMSQLDAVAMSRGPGSYTGLRIGASVAKGLCYGLNIPLIAVSTLQAMAL
                     QAARILQISPQEDVVFIPMIDARRMEVYSAVYNFRGEELREVRAEIITADLFEDIHSR
                     IVICGDGAAKCRQLFENDLRFIIMERLLASAAWLVPLAWENFVKGKFENTAYFEPYYL
                     KDFIAGIPHIKGLK"
     gene            7289..>7664
                     /locus_tag="PLF20_07725"
     CDS             7289..>7664
                     /locus_tag="PLF20_07725"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HOX80924.1"
                     /translation="MNIEKNVNLAIFASGSGTNAENIIKYFKDHGYIRVIRVYCNNPQ
                     AYVINRVKPYEIPVTLFGKEDFVENGLVHQKLKEELIDYIILAGFLWKIPDFLIQQFS
                     GRILNIHPALLPKYGGKGMYGER"
BASE COUNT         2253 a         1452 c         1545 g         2414 t
ORIGIN      
        1 caagaaaagc agcaaaaagc taaactgtgt aaaaattttg tttttcattg gtaaaatgga
       61 attgatttct tgagttcact tcgagaattt cttgcgcaaa attagtacta tttcttcggt
      121 atgttaatgt aatgtatgcg atgaaaatca tttttttaga aatgaaatgt tattcgactc
      181 tcagataagc aatgaatgat ttttgagtac tttttcttgc tggaatccat gaggcgatta
      241 tcccaataat cacaacgaag acgaaaacca ctaaaaagtc cattccctgc atttcaacgg
      301 ggtattgact aataacgaat gaacttccgc tagtgttgat cttgacgaat ccgaaagttt
      361 gctgtaaaaa gcaaatgaag gctcccagca gtgtaccccc tatgcctcca gctacactaa
      421 tccataatcc ttgatataga aatactttcc gaatatagga tgtaggagct cccatggcca
      481 tgaaaatagc tatgtctctt tgtttttcgg aaataaccat cgaaagcgaa ccgataagcg
      541 taaatgtggc cacaatcaaa ataaaggcaa ggataatgaa aatagcatat ttttcggttc
      601 gcatgatttt atataacgaa gcttgctgtt cgtagcgatt ggcaactttg aaccccgaac
      661 ccatcatctc cgaaatcttc ttttgtatag attttatgtc ggcatttggt tttaaataga
      721 cttcaaaagc actcacctct gttgaatatt ccagtaactt ccgcgcaaat gaaaggggag
      781 taattacgta ggaaatatcg aaatcctgtt gtacgctaaa caatgcagaa ggtctgatag
      841 caagtgagtt gaaggcttct tccgaaaata aagccgatag attcgatttt cgggaaggaa
      901 caaatacctc gaggggatag aacgcatcgt ctaaacccat attcaaataa taggctactc
      961 ccgctcccac aaccgcataa ggacccgttg aatcgctcag tacgaattca ccttgaagaa
     1021 ttttttctct aagatgagag aacagcgggt aggtctgatc gactcctttg atcaaaccaa
     1081 tatgttgatg atttctatat cggaataaag cattgtcttc gatggtttca cttatacctg
     1141 caacgcctgc catagaagaa attctgttct tgatgtcttc gttgggtttg aatacctttc
     1201 ctttcactgc cgtgatttta atatcgggat cgaaaacgtt atagagactt tgaattacct
     1261 gctgaaagcc attgaaaacc gaaaggatga taaccagagc agcggtcccg gttaatatgc
     1321 ctgctacaga tatccccgaa acaaaattaa tggcatggtg agtttttttg cctaataaat
     1381 acctcaaaga tatagaaatg ggcagcttca taccttcctc atatttattt ccaggccttg
     1441 acaatcaatc ccgttccgct ggaatgcttt cgacttatgt ccatttgatt cagcaaaagt
     1501 gcaaacggaa aagcgatcaa atagtaaaat ggaagaagaa tgaagaataa tttactgaga
     1561 ttgagcataa cgatagggta tttcatggaa agattccatg aaatttttcc tggtttgcca
     1621 taagtgtaat aggcttcggt gcatgaaaat cctgcttccc ttagtttttt ctgaatcata
     1681 aggatattgt agccatctct tacatgttca cctataaagg aatggctggc attctcagag
     1741 atatcctcat cgtgagggtg aacatcggag ccgccttgat cgcttggtgt tgagatcaga
     1801 agcattcctc cgggttttaa ggcatgaaag aaattctgaa agacagcctc atcgtttgga
     1861 atatgctcca tcacgtcaat cgataaaatg aggtcgaata aaggaggtga agaaaatctt
     1921 tcgagatcag ccacttcgaa tctggcattt ttcaatcctg cttttctaaa gaaggtatta
     1981 cagtcatcta cctgatcgtc tttaatatct acccccagga tttcccaatt ggggtgatgt
     2041 tgagccagaa gataggaata ttgcccgaag cccatgcctg catcaagtat cttggccgtt
     2101 tctcgatgtt gcgattccca aattcgaagc tcttttttta catgccaggt acgtaataaa
     2161 agtaagttca acaatcggta aaaaagaatg cgtaagcgag gagttcgatt gaaaaacctt
     2221 cccaaatccg atttaatcgg gtcgtattgc ataaggttta ttggttcaaa agtttatcaa
     2281 tgcgttcaat ataatctaaa ctatcgtcga gaaaaaattg aagttgaggg atgatacgta
     2341 cctgatgcct gattttgact ccaagctgat agcgtaattc tggcccgtgc aatttgagct
     2401 gttcgagagc ggctttctta tcgggcagac caaatatact aatgtggata cgtgcaatct
     2461 gcaaatcggg tgtaacactt acacgggtta ccgtaagtat gcctcctcct gtataaggga
     2521 cagcatcctg cagcataaga ctcatttcct tttgtatgag cctggctacc ttttccaatc
     2581 ttgtcttttc cattttctaa cttccctgtt caataagctt tggcttagat catttttaat
     2641 catctgacga ttttcggaat ttttgcaaaa ttatcgaaaa aatactacaa atttttggct
     2701 ttgcatcttc tatcattgaa aatatgggaa tagttagaaa tatggctaga ttagcggagc
     2761 atttagcttc aggaaaattc acctgcgaat actgtagtga gaattatctt attcgaaggc
     2821 tccaaccatt actccttcgt ccatttcccc tgctctatgg ttttgccgtg atattggata
     2881 ttccataaat acaattcaga aggtagaaat gaagtggaga gccgttgtgt ttgggtttgt
     2941 agccagctgc gtaacacgag cttacctgac aaattgtaga gcaaaatggc ttcgctaatc
     3001 tgaggcccgc tttcgatgat ttgataatcc ttgacgggat tgggatacac tatggcatcg
     3061 tgggacgagt attcttaccc cttcatctga tgatggaatc aaccctaagg aatcggtttt
     3121 gatagatacg atttcttggt ggtaatcatt attccaccat tcatattgag ttcctcccag
     3181 caaacaccca ccgtcactgg tcgctaggat atcccaaagg ccataatata catttgctcc
     3241 ataaaacctt tgccattcca atccgagcgt atcatttaac atggcgatac aataccatga
     3301 gggctttgat gaaaataagg tattgtagtc ttggtttttc gtaaatccac aaaaatcctg
     3361 tttgcttcga tatatgaaat attatttata tatgcgggat aatctaccgt atcttttgct
     3421 ccaagataag attcatataa aatattatgt aaattcaaaa catcttatta tatatacatt
     3481 aatcattttt gagggcaaaa ggtaaccccc caaagataaa aatttttcaa caaatacaaa
     3541 atttttttta gaatcttgct cttcatggtt tagaaaattt agaccttcaa aagataaaac
     3601 taaaatttta aaaaacaagg gatttatatg ggaaaaaccg gagaaaagga ggaagcgaga
     3661 cggggggact gggagacgag gagacgaggg gacagggagg atggtggata gataagttaa
     3721 aagtttataa agtcaaaaac ggtcaacata tttcaggcac cgatatccgg catttaatct
     3781 tcccttctat cggggtgatc attgagaaaa gccttccaac ctgtatattt tttgacttca
     3841 gaatgaggat catcttgaaa ataatgacaa actgcggcgg ccaaggcatc ggtggcatcg
     3901 agagattcgg gagtttctgc tatagtgatc aaacgaccta acatggcagc aacctgctgt
     3961 ttagaagcat taccattgcc cgtaaccgat tgtttgattt ttttgggaga gtattcaaag
     4021 atgggaatat ttctgtaaag agcggctgca atggctactc cctgagccct tcctaatttt
     4081 aacatgctct gcacattctt tccgtaaaat ggggcttcaa tggccaactc atcgggcttg
     4141 tattcatcga taagtgatag cgtacgttcg aatattctct ttaattttaa cggctgattg
     4201 gcaatcctgg aaagacgcaa taccccaagg gttaataact ctatttcgcc tttctggcat
     4261 tctatcaaac cataacccat aatagtagta cccgggtcta tccctaaaat gattctatct
     4321 tgccctgtca aaatttcaaa ctttagtaat gacaaaagta accaaaagaa acatcaacat
     4381 ttttcttcag attttaatcc tgataggagc ttatggttat ttggcgtgga aattatttat
     4441 agctccttct actcgtctca ctgcacaaaa tatttctcaa ctttttcata tctcgaattt
     4501 ctggctggtc atcagtatcg tcttgttgct catgctcgtc aattggagca tagaagccgc
     4561 taaatggaaa tatttaattt cgaaaatcga agaagtaagc tttcccctag ctttgaaagc
     4621 tatctttacg ggagttaccg taagtatttt tactcctaac cgtgtaggag aattttttgg
     4681 aagagttttc cttcttaaga atagacaacc catgagaggc attctcatta ctattttagg
     4741 aagtttttct caaatcgtaa tttacatgat ttgcggcatt acaggtggaa tcatcatgtt
     4801 ttatcttttc ctgctgcctc aaaatccttc tctaacaaaa cttcaatgga tcattatatc
     4861 gttagcggtt attatcttgg ccgtaaccat actcatcttc ctgaatattt catttttggg
     4921 aagtattctt cagaacctca ttcccaaaca atggcacaga ataaaacgct ggtttgctat
     4981 actatcgtat tattccaaaa gagaattgac caaggttctc atcatgagct ttctacgtta
     5041 ttgcgtcttt tctttccagt atattatttt acttcgaatg ttcaatgtcc ctttgagcgt
     5101 ggggaactta ctcgtggcac ttatggttat ctttgcctta ttagtcatca ttccttctgt
     5161 tgcgctttcc gaattgggag taagaagtac ggtagccgtt tttgttattg gtatgatgtt
     5221 gcctgtaggc cttcgcaacg atcccatggt gaatctgggg ataataggtg cagccagcac
     5281 tttatggctt atcaatgtag ccttgcctgc tatcataggc agctttttcg tgttcaaact
     5341 caaattcttc aaaggcaatg gtcccaaatc ataaggcaat atggataagt tatcattctt
     5401 gatcttgatt attccttgca tttatgccct gatgatgatt gcctttgctt ttgggtggtt
     5461 gtggcttccg gcttatcgga acgaatgtcg aaaaagtgta agcatcatta tcgctgtaag
     5521 aaatgaagaa gaaaacattc ctcaacttct gcaaggcctc catttgcaat cgtatcctac
     5581 tcatctcctt caaatcatca ttgttgatga ccattctaca gatcgtacct ttcaattgat
     5641 cgagaatgac cctttggttc agaatattcg cttgccttcg aacttgcaag ggaaaaaggc
     5701 agccatacaa aaggggatag aagaagccaa gggtgaaatt cttcttttta ccgatgccga
     5761 ttgtttttta ccacctgatt gggtaacaag catgattatg ccatttgcta acgagaaaat
     5821 acatcttgtg gctggccctg tacagataaa aggagagggc ttatgggcta aaattcagga
     5881 aattgaattc tacagcctga tggcagtcac tgctggaagc attggcataa ggttacccct
     5941 catgaataat gcatcgaata tggccgtcag gaaacaatcg tggtacgagg cagtacttat
     6001 gggtttagga aagaagttcg tttcagggga tgatgttttt atgatgatgt cgatagcaaa
     6061 gatttttggg tcaagaagca ttactttcct caagtctaat aaggctattg ttgttaccaa
     6121 agccttacga aatatatccg atttttttcg acagcgtatg cgttgggctt ccaaaaccaa
     6181 ggggtattct gcaattcttc ctcgattggt ggcttttatt gttttgctga caaaccttct
     6241 tttcttgata tccatgttct gggctatttt ttatttccct gccttcagcc ttaccatcga
     6301 aatatttatc gtaaaatcct tggctgacct cgtggtaatg atccctgctc tgcgattctt
     6361 tcaatcactt tggaaaattc ctgccattat ttttcttcag cctttttatc ctttctatgt
     6421 tgtgagtgtt gccttaatga gtctttgggc gagaaacgta tggaaaggtc gccgaatata
     6481 aaaggtttag gctaattttg tcccatcgaa agtaaaatga ggtatttcaa taaaaatttt
     6541 agataaaatt ctcgaaaaga gaataaacaa atactggaga tattttttaa agaatgacaa
     6601 atttgctgtg catagaaagt tcgacgtcta tttgttccgt agcattggca aatgaagggc
     6661 agcttatggg attggaagag accgaagaca agaatgctca ttcggcgcgg cttacggttt
     6721 taattgaaaa attattgaaa gataataact taaagatgag tcaattggat gcagttgcca
     6781 tgagtcgtgg tccgggttct tatactggat tgcgtattgg ggcttctgtt gcaaagggtt
     6841 tgtgctatgg tttgaacatt cccttgattg ccgtgagtac tttacaggct atggctttgc
     6901 aagccgcaag aattttacag atttctcctc aagaggatgt agtttttatt cccatgatag
     6961 atgcgagaag gatggaagta tattctgctg tttataactt taggggtgaa gaactaagag
     7021 aagtaagggc agaaattatt actgcagatt tatttgagga tattcattcc agaattgtta
     7081 tttgtggaga tggagcagca aagtgccggc aactttttga aaacgatctg cggttcatca
     7141 ttatggaaag gcttctcgcc tctgctgctt ggttagttcc tctggcctgg gaaaatttcg
     7201 tgaagggaaa gttcgaaaat accgcttatt tcgaacctta ttatttaaag gattttattg
     7261 ccggaatacc tcatatcaaa gggctgaaat gaatatagag aaaaatgtta atctggctat
     7321 atttgcttcg ggcagtggta ctaatgccga aaatatcata aaatacttca aagaccatgg
     7381 atatattcgg gtaataaggg tttattgcaa caatcctcaa gcttatgtga taaatcgtgt
     7441 aaaaccatac gagattcccg ttaccctctt tggtaaggaa gatttcgtcg aaaatggctt
     7501 agtgcaccaa aaactgaaag aagaactcat cgattatatc attcttgcag gttttttatg
     7561 gaaaattcct gattttctca ttcaacaatt ttctgggcgc attcttaata ttcatcctgc
     7621 tcttttgcct aagtatggcg gcaaaggtat gtatggcgaa aggg
//
LOCUS       DAOORU010000117         7591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_11928_length_7591_cov_4.561040, whole genome shotgun sequence.
ACCESSION   DAOORU010000117 DAOORU010000000
VERSION     DAOORU010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11928_length_7591_cov_4.561040"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="PLF20_07730"
     CDS             <1..172
                     /locus_tag="PLF20_07730"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOX80925.1"
                     /translation="RHERRVELAQEFHRYFDLMRWGKQTAEEALGSGFSYENNRYFPI
                     PQNEIDANEAIP"
     gene            225..1298
                     /locus_tag="PLF20_07735"
     CDS             225..1298
                     /locus_tag="PLF20_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80926.1"
                     /translation="MFKKASILLQSLMLILMMSTATLFVACGDDEDEVVAGDKTQLNA
                     LIADLETIANSATTADYPQAAIDEFKAAIQTAKTAAQGTLTQEQINNLITQLNEAKAS
                     FESKAYGYIDESLYLIMGWHFDEGDGTTTTDYSSNHYVGTFKRGSVDVLGEDALMPSW
                     TNNGLKGKAIYYNKGAHLEVPYASAFLPSNLTISVWIKPDELYEHNYIISQNYWNGYK
                     LQTQGAGKPFFTYKKADGNIIDADNETDNSVKAGQWNHIVITLDGTAKLLKFYVNGTL
                     TKTWDEATKGIGPLTQTLTNPDPQPFIIGGVATDAELLANFMEWTTAENLGYFKGIID
                     ELRLYNIALTEGQVTKLYNSDKP"
     gene            1427..4525
                     /locus_tag="PLF20_07740"
     CDS             1427..4525
                     /locus_tag="PLF20_07740"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80927.1"
                     /translation="MKHSFILSLLLLASIYSTAQTGQININRIEQMPNLPSDFLVRNW
                     HKVALDYDQFVFDTTRQGQYLPFSRLGMLGQFNYPDNRPIFLDTYVGSSWHHQQAEAI
                     NLMPAVIGASLVGIDKSNQNGMNYVAALKDFFNLKNGQNVYLNNYFASSGSDWWYDLM
                     PNVYFYQLKWLYPDEAPEFEYQYSEVAERWLGCVYALGGNTKPWQIPNMNYRAFNLIT
                     GKPLSTGVPEPEASGAIAWLLLQAYFTTHQEEFRQGAELAMEFLDGLTSNPSYELQLS
                     YGVTAAAAMNAIVGTNYNLTKMLSWCFDRGNLRGWGANVGKWGEVEVNGLIGEVNENE
                     GYAFVMNGFQQAGALTPLAKYDKRYARTLGKWLANLASSSRYFYWNALPSDYQDSYSW
                     ASQYDTSACIPYEALKSTWQGKSPFARGDAIAGGWAATNLSLYSGSSVGYLGAMIKRT
                     SVDQILAFDLNQTDFLVKDSIPAFLLYNPNDSNLTCSFDLPSGNWKVYESLTETFFPE
                     VISGSFTLNIPADEACLIRLYPASETCTIEGNHLVCNGKIIDYQYHYNFSKKLRIKAL
                     ESEPNPVEKNASFRVWVKADQIPEGVVPVYFWNFNGTQITTLVDSTHLKTPSQTGLYV
                     LTLKVQAGDQWAEDSLRIEVVDWIPKPPLIDSISVSKFYCNPLEKITLEIWADTTGSG
                     PYNFSWTSSAGQISFSNNIAEFVAPSAEQIVSITGKITNSLGLSSTKSIEILVRDTLH
                     HSAQPLIWYPFDTSDSNAVTNKFHATSIGTIKAPDARNIENKAKRFISNSDLIYTENE
                     PELDFSYPLTLSCWIYPEKIYQEHYIISHGSWQERFKLSLLSDHHLRLTVNTTSGIFD
                     LDSKNPIPLEQYTHVTGIYTGFSLEIYINGKLDNFMSCSGNLNPTSHPLTLGRMNEIE
                     TKYSFWGRIDEFKLWNDALSLSEVKNLPFIWFSLADGQKLHVYPTLLRKGESIKISGI
                     EAGSEVKLYSVFGQVVGINLQINPGETSNVRFPAYIRTGMYLLRCNQPSKGTEVVKIV
                     IY"
     gene            complement(4891..6096)
                     /locus_tag="PLF20_07745"
     CDS             complement(4891..6096)
                     /locus_tag="PLF20_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOX80928.1"
                     /translation="MPEISEKGKKMPASPIRKLVPFADKAKQRGIQVFHLNIGQPDIE
                     TPQTILDAIHHFDQKVIEYSHSAGFLSYRTKLTQYYAKLGISIQPDDIIITTGGSEAI
                     LFALQSTMDPGDEIIIPEPFYANYNGFAINSGVIVKPIPSFIENGFALPAISEFEKAI
                     GPRTKAIMICNPNNPTGYLYSPEELELLRDIALKYNLYLFADEVYREFCYDGREHFSI
                     MQLEGLDQHAVLIDSVSKRYSACGIRIGCIISKNKQLMATAMKFAQARLSPPSIGQVA
                     AEAALDTPAQYFENVRNEYFARRNVVVEAINKMPGAFCTSPQGAFYVMARLPIDDSDL
                     FCKWLLEDFESKGRTVMIAPGTGFYSTPGKGIDEVRISYVLRVDELQQAMECLAEALT
                     IYPHRKHCK"
     gene            complement(6256..6741)
                     /locus_tag="PLF20_07750"
     CDS             complement(6256..6741)
                     /locus_tag="PLF20_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HOX80929.1"
                     /translation="MKRKLIFVLGIFLTLNYLQAQDAKPVSKNGPIITFDQKLRDEAG
                     NIVWDYGTIYKDSKGDSYFRFTNTGKEPLVLSEVRSSCGCTVPSWPREPILPGKSDTI
                     KVRYATNRLGVINKTITVMSNAVNNPVILRIKGNVIEKPAEVVPEKRSVDAAQPLNKA
                     K"
     gene            7206..7580
                     /locus_tag="PLF20_07755"
     CDS             7206..7580
                     /locus_tag="PLF20_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOX80930.1"
                     /translation="MENQIDTGLLHIPATEPIVIFAIILLSILLSQTIFRKIKLPAII
                     GYILAGIALGPHGWGILQNDSSIQLFGNAGLLFIMFVAGLDLNLQEFKHTAYKSIIFG
                     LLTFSIPLSLCFLSCRTLLHLD"
BASE COUNT         2214 a         1639 c         1477 g         2261 t
ORIGIN      
        1 tcgccatgaa cgcagagtag aactcgccca agagtttcat cgctatttcg acctcatgcg
       61 ctggggaaag caaaccgcag aagaagctct tggcagtgga ttttcatacg aaaataaccg
      121 ttatttcccc attccacaga atgaaataga tgctaatgaa gcaattccgt aacctttatt
      181 attaaataat taatttatta ttaacctttt aaaactcaag acctatgttt aaaaaagcaa
      241 gtattttact tcagtcctta atgctcatcc taatgatgag cacagctaca ctatttgtag
      301 cctgcggtga tgatgaagat gaggttgtgg caggcgataa aacccaactc aatgccctga
      361 ttgccgattt ggaaaccata gccaatagcg ctaccactgc cgattacccc caagctgcca
      421 ttgatgagtt caaagccgct attcaaactg caaagactgc agctcagggc acactcactc
      481 aagaacagat caacaacctg attactcagc ttaatgaagc caaagcctca ttcgaatcga
      541 aagcctatgg ctatatcgat gaatctctct acctgatcat gggatggcat tttgatgaag
      601 gagatggaac taccactacc gattattcat ctaaccatta tgtcggtacc tttaagagag
      661 gtagcgtcga tgttttggga gaggatgcct taatgccttc atggactaat aatggattga
      721 aaggcaaagc catttattac aataagggcg ctcatctgga agtgccttat gcatcggctt
      781 tcctgccttc taatcttacc atttctgttt ggataaaacc cgatgaactt tacgaacaca
      841 actacatcat atcacaaaac tactggaatg gttataaact tcagacccag ggagcaggca
      901 agcctttctt tacctataaa aaggccgatg gtaacataat cgatgccgac aacgaaaccg
      961 ataactctgt aaaggccggt cagtggaatc acatcgttat aacccttgat ggaactgcca
     1021 agctattgaa gttttatgtg aatggtactt taacaaagac ttgggatgaa gccactaaag
     1081 gcattggtcc tctcactcaa acgcttacca atcccgatcc tcaacccttc atcattggcg
     1141 gagttgctac cgatgccgag ctgttggcca atttcatgga atggacaacg gccgaaaatc
     1201 tcggttattt caaaggcata atcgatgagt tgaggcttta taacattgcc ttgactgaag
     1261 gacaggttac caaactttat aattcggata agccctgaac gcattttgtg cgattttggt
     1321 tagtcatggt ttaacaatgt tgtgtttttt cgggccagcc ttcggctggc ccgatttttg
     1381 ccccttcctt tcatcgtcct attcttgatt atgttactcc cttcttatga aacattcctt
     1441 tatcctttct cttcttttac tcgcctcaat ttattcaaca gcccaaacgg gtcagataaa
     1501 catcaaccgt atcgaacaaa tgcccaacct cccttctgac ttcttagtca gaaactggca
     1561 taaggttgct ctcgactatg atcaattcgt attcgatacc actcgtcaag gtcaatatct
     1621 tccattttca agactcggaa tgttagggca gtttaattat cccgacaatc gtccgatatt
     1681 tcttgatact tatgttggca gtagctggca tcaccagcaa gccgaagcca tcaacctcat
     1741 gccggctgtt attggggcaa gtctggttgg tattgacaag tcgaatcaga atggaatgaa
     1801 ttacgtggcg gctttgaaag attttttcaa tctgaaaaat ggccaaaatg tgtacctcaa
     1861 caattatttt gcttcttcag gctctgactg gtggtacgac ttaatgccca atgtttattt
     1921 ttatcagttg aaatggttat atcccgatga agcaccagaa ttcgaatatc aatattctga
     1981 agtggcagaa cgatggttgg gttgtgtata tgccttggga gggaacacca agccttggca
     2041 aattcccaat atgaattaca gggccttcaa tctgataact gggaaacctc tttccacggg
     2101 tgttcccgaa cctgaagcct cgggagctat tgcatggtta ctgcttcagg catattttac
     2161 aacccatcag gaagaatttc gccaaggagc cgaattagcc atggaatttc tcgatggttt
     2221 aacctctaat ccctcttacg agcttcaact ttcttatgga gtaacggctg ctgctgccat
     2281 gaatgccata gtgggaacca attacaatct cacaaaaatg ctcagttggt gtttcgatcg
     2341 tggaaatttg cgaggctggg gcgccaatgt cgggaaatgg ggtgaagtcg aggtaaatgg
     2401 cctcattggt gaagtaaatg aaaacgaagg atatgctttt gtgatgaatg gatttcagca
     2461 ggcaggtgca cttacacccc tcgcaaaata cgataaacgt tatgctcgca cattgggtaa
     2521 atggttagcc aatctggcca gctcctcaag atatttttac tggaatgctc ttccatccga
     2581 ttatcaagat agttattcat gggcttcaca atatgatact tcggcttgca tcccttatga
     2641 agctctaaaa tcgacttggc aaggtaaatc gccttttgcc agaggtgatg ccatagctgg
     2701 aggctgggct gctaccaatc tttctctata ttcaggatca agtgttggct atctgggcgc
     2761 aatgatcaaa agaacttcgg tagaccagat cctcgctttc gatctgaatc agactgattt
     2821 tctggttaaa gattctatcc ctgcattttt actctataac cctaacgatt cgaaccttac
     2881 atgcagcttc gatcttccct caggaaactg gaaggtatac gaaagcctta ccgaaacctt
     2941 tttccctgaa gtaatctccg gttcgtttac actgaatatt cccgccgacg aagcctgttt
     3001 gataagatta tatccagcat cagagacttg cacaatagag ggaaatcact tggtttgtaa
     3061 tggaaaaatc atcgattacc aatatcatta taatttctcg aagaaattac gcatcaaggc
     3121 attggaatca gaacccaatc ccgttgaaaa aaatgcttca tttcgcgtct gggtcaaagc
     3181 cgaccagatt cccgaaggtg tagttcctgt ctatttttgg aatttcaatg gaacacagat
     3241 tacaaccctc gtcgattcca ctcatttaaa aactccctcg caaaccggtc tgtatgtttt
     3301 gactctcaaa gtacaggcag gagaccagtg ggctgaagat tcccttcgaa tcgaagtggt
     3361 cgattggatt cctaaacccc ctctcatcga ttctatcagc gtttccaaat tttactgcaa
     3421 tccactggaa aagatcacgc tggagatatg ggctgatacc acagggagcg ggccttacaa
     3481 tttttcatgg acatcaagtg ctggtcagat atctttttcg aataatattg ctgaatttgt
     3541 ggcaccttcg gccgaacaga tcgtcagcat caccgggaaa ataaccaatt ctttgggatt
     3601 aagctctacg aaatcgatcg aaatattggt tagagatacc ttgcatcatt cggctcaacc
     3661 cttaatctgg tatcctttcg acacgagtga tagcaatgcc gtaaccaata agtttcacgc
     3721 cacttctata ggcacaatca aggcacccga tgcccgaaac atcgaaaata aagctaaacg
     3781 tttcattagc aacagcgatt tgatttacac cgaaaatgaa cccgaactcg acttttctta
     3841 tcccttgact ctgagctgct ggatatatcc cgaaaaaata tatcaggaac attatatcat
     3901 ctctcatggt agctggcagg aaaggttcaa actgtcgctt ttgtctgatc atcacctcag
     3961 gctgacggtc aatacgacta gcggaatttt cgacctcgac agcaaaaacc ccattccctt
     4021 ggaacaatat acccatgtaa cgggcattta tactggtttt tcgcttgaaa tttacatcaa
     4081 tgggaagtta gacaatttca tgtcgtgcag cggtaatctt aacccaacct cccatcctct
     4141 taccttgggt cgaatgaatg aaatagaaac caagtatagt ttttggggtc gtatcgacga
     4201 gtttaaactt tggaatgatg cattgtcact ttccgaggta aaaaatcttc ctttcatctg
     4261 gttttcttta gccgatgggc aaaaattaca tgtttatccc actttgctta ggaaaggaga
     4321 aagtataaaa atttccggaa tagaagcagg ctctgaagtc aaattatatt ccgtgtttgg
     4381 acaagtcgtc ggcataaatt tgcaaataaa cccgggcgaa acttcaaatg tcaggttccc
     4441 ggcatacatc agaaccggaa tgtacttgct acgctgcaat caaccatcca aaggaaccga
     4501 agtagttaag atcgtcattt attgactggc tcaatgctga aaaccgatgg ctttcccaag
     4561 gagaacttat tcgaatttaa aatgcttcac atgaatgctg agaatgtcaa atccggataa
     4621 atttgttcga actacatctt ttgaaaagca aaaatcatcc ttgattcttt attatccgaa
     4681 catagaatct tctacttgaa tttccttgag cctatgttca atcattattt ccgatgaaat
     4741 ttgcttcatc taatcaattt ttctcaccga cagcatcatc aataaatttt gttcattttt
     4801 acctttttaa aaggtataac caataaaaac atcaagctgg ttcaatggat atttttccaa
     4861 aaatccagct caagttcaag aaaaaaaata tcatttgcaa tgctttcggt gggggtaaat
     4921 tgtcaaggct tctgccaggc attccatagc ctgttgtagt tcatcaactc taagcacata
     4981 cgagattctg acctcatcga ttcctttacc tggagtagaa taaaaacccg tacccggcgc
     5041 tatcatcacc gtacgtcctt tcgattcaaa atcttcgaga agccatttgc aaaagaggtc
     5101 actatcatca atcggtaatc gagccattac ataaaaagct ccctgagggc tggtacaaaa
     5161 tgcgcctggc attttattga tggcttcgac taccacattg cgtcgggcaa aatattcatt
     5221 cctgacattt tcaaaatatt gggctggtgt atcgagcgca gcttcggcgg ctacctgacc
     5281 gatacttgga gggctcaatc tggcctgagc aaatttcatg gcagtagcca tcaattgctt
     5341 attcttcgaa attatgcatc ctatccttat tccacaggca ctgtaacgtt tgctgaccga
     5401 atcgatcaaa acggcatgct gatctaatcc ttccagctgc ataatactga aatgttcacg
     5461 tccatcataa caaaattccc tgtacacttc atcggcaaaa agatataagt tgtacttcaa
     5521 ggcaatgtct cggagtaatt caagctcttc gggactatat aagtaacctg tagggttatt
     5581 cggattgcaa atcataatgg ccttggtacg gggtccaatt gccttttcga actcactgat
     5641 ggcaggaagg gcaaaaccat tttcgataaa ggaagggata ggttttacga tgactcccga
     5701 attaatggca aaaccattgt aattggcata gaatggctcg ggaattataa tttcatctcc
     5761 aggatccatg gtagattgca gagcaaataa gattgcttcc gagcctcctg tcgtaatgat
     5821 gatatcatcg ggttggattg aaatacccag cttggcataa tactgagtga gcttggtccg
     5881 gtacgacaaa aatccggctg aatggctata ttcgatgacc ttctggtcga aatggtgaat
     5941 agcatccaag attgtttggg gtgtttcgat atcgggttga ccaatattca gatgaaacac
     6001 ctggatacct ctctgtttgg ccttatcggc aaatggaacg agtttgcgaa tgggagaagc
     6061 tggcatcttc ttgcctttct ctgatatctc tggcatagca atgaatttaa agttaagtaa
     6121 ccgcaaaggt aaacaaatac taccatttta aattttacag acaacgcaac atctattgaa
     6181 atctcctcaa acctacaccc acaaaaaaac ccagaggtac attctccggg catgagtatt
     6241 cttcaagatt agagtttatt tggctttatt caaaggttga gcagcatcga ccgacctctt
     6301 ctctggaact acttcggcag gcttttcgat tacattgccc ttgatgcgta aaattactgg
     6361 attattcact gcattcgaca tcaccgtaat agtcttattg attactccta aacgatttgt
     6421 agcatacctt acctttatgg tatcactttt acccgggagt atgggttcac ggggccagct
     6481 gggcacagta catccacacg aagaacgtac ttcggaaagc accagaggct ctttaccggt
     6541 attggtaaac cggaaataag aatctccttt gctatcctta tatattgtac cataatccca
     6601 tactatattc cctgcttcat cacgcagttt ttgatcgaaa gtaataatgg gaccattttt
     6661 gctgactggt ttcgcatcct gcgcctgtaa ataattcaag gtcaggaaaa ttcctaaaac
     6721 gaatattaat ttgcgtttca tagtatatag ctgattttta aggtttgcat ttcgtttcaa
     6781 ttcatacgac cgcaaaagta atgaatttcg aggctatggt taaaattttc gaacttgttc
     6841 actaaaattt taatcttttc ccccttcatt ttgttaaaat tttgtttcat gatgttttat
     6901 gaatgaatcc attctcgaac ttaataagaa acaaccttcg gagaattaca gaaattatgc
     6961 cataaccttt aagaattgaa aaagaatggg aaggagtaga aaaaatttgg tcgattttat
     7021 aaaacatgct gacgttaaaa aaggatacta tggaatagga cacggctaat tcgataatga
     7081 ttcagtgata aatggagatt tctttgattt tttaaataat ttatatacag caaattaaac
     7141 taaacatcat tttcctattc ggctttatgt tctaattttg tatttcattt ttcgctcaaa
     7201 taaggatgga aaatcaaata gacaccggct tattacatat tcctgcaaca gagccgatag
     7261 taatttttgc catcatctta ctgagtattc ttctttcgca aaccatattt cgaaagatta
     7321 agctaccggc gatcattgga tacattttag ccggaatagc gcttgggcct cacggttggg
     7381 gtattttaca aaatgacagc agcattcaat tgtttggtaa tgcgggcttg ttattcatca
     7441 tgtttgtggc cgggttagac ctcaatctac aggaattcaa acatacagct tacaagagta
     7501 ttatttttgg attgttaaca ttttctattc cgttgtcgct gtgtttttta tcgtgccgca
     7561 ccttacttca tctcgattaa ttaccatctt t
//
LOCUS       DAOORU010000118         7570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_11974_length_7570_cov_5.340652, whole genome shotgun sequence.
ACCESSION   DAOORU010000118 DAOORU010000000
VERSION     DAOORU010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11974_length_7570_cov_5.340652"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1518)
                     /locus_tag="PLF20_07760"
     CDS             complement(<1..1518)
                     /locus_tag="PLF20_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HOX80931.1"
                     /translation="MDENIQNEELFPEDSIKENETSASSEYRTITLRGMYENWFLDYA
                     TYVIMERAIPEMADGLKPVQRRLLHAMYEMDDGRYNKVANIIGQTMKYHPHGDQSIGD
                     ALIQLGQKELLIDCQGNWGNIFTGDQAAAPRYIEARLSKFSLEVVFNPKTTQWKLSYD
                     GRNKEPVTLPVKFPLLLAQGVEGIAVGLASKILPHNFNELIKASIKILNNEDFEIFPD
                     FQTGGLADCSKYNRGLRGGKIRVRARIVQLDKKTLAITEIPYGVTTSSLIDSILTAND
                     KGKIKIRKIEDNTAENVEILVHIASGVSPDQTIDALYAFTDCEVNISPNATVILNGKP
                     AFIDVNEILRINTQNTVELLKKELLIRLNELEDDWHHSSLEKIFIEKEIYLQIRQSKS
                     FEEAISTIQKGLKPFLQLLHREVTYEDITHLTEIRIKRISAYNTFEADRHIESIENEM
                     QKVKERLENLIDHAIEWFEHIGNKYGIHNERKTELRSFDTIEAAAVAATNEKLYVN"
     gene            complement(1551..2786)
                     /locus_tag="PLF20_07765"
     CDS             complement(1551..2786)
                     /locus_tag="PLF20_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005867620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOX80932.1"
                     /translation="MIYPKIRLSENIYWVGTNDRKTRLFENYWPIPSGIAYNSYLILD
                     DQITLMDTVSMGTMDSFLEKLKEILNGRAINYLIINHMEPDHSGAVKTIINEFPQVTI
                     VGNNRTFPMLEGYYGIKKNLMEVEENAVLHTGQHQFHFYLTPMVHWPETMMTYESTEK
                     ILFSADAFGSYGTLDGGIFDDELNLSFYEDEMRRYYSNIVGKYGLQVQKALAKLESLD
                     LRMIASTHGPIWRLHIPKVVEFYRNWSTYKTEQGVVIAFGSMYGNTEKMADIIARAIA
                     EEDIRNVRIYDVSKTHSSYIISDIWKYRGVILGSCAYNGGIFTPMRDLLMQLEHIMPR
                     NHFLSIFGSMSWGGGGVSTLKKFAENIKWELIGEAIEVKHSPSDKDIVSLIELGKQMA
                     RRLKENDSSFSENNKPEMI"
     gene            complement(2802..4844)
                     /locus_tag="PLF20_07770"
     CDS             complement(2802..4844)
                     /locus_tag="PLF20_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOX80933.1"
                     /translation="MEFIYPWYLVGLAAISIPIVIHLFNFHRYKKVYFTNVRFLQAIQ
                     SETHRSSRLRNLILLLLRILFIITLVLAFAQPYIPASNNIKQGNPFAVSIYLDNSQSM
                     ETGMGNENLLHQAREKALEIVNSFSSTDKFQVLTNDLEGKYQHFVPANEASIWINDVK
                     SSLAQVSTSTLLQTQANLLRYEKNYNHLAFFISDFARSTFSLPTNLDTNITWIFIPMQ
                     SPLPLNVSIDTCWFDTPIIAPGLESVLRVNVTNHSANQSRELMVKLSTNEIQRGLKPL
                     SLTPKTTFTLSFPLFNADTGTIMGSLQINDNGFTFDDELYFAYTVHPKLEILIVNNSE
                     GENPHLNQLFHSDSLMWVDNQPIHQLIYSRLEKSSLTILNELTEYPQGLILSVRHSLN
                     AGHSVAILPHPSKGIDHLERFLSNLGAGLELHADTSSTTVSQVNTDHFIFKPAIERMP
                     NMPLLPSVKKYFKINLNSRSGYDNLMTLRNGDPFLIAKRLGNGHLFFQASPLRPEFTD
                     FMRNNLFVAAYLNMAISGQYQNPLYAVYGKPSIFYFEKPAETTPAPHLIKSDNTIDII
                     PFFRDRDGQTEIIVRNELPEPGIYHLRRNNKTNGFIALNAPRTESLPTCFSVDELDRM
                     LEQKGWTNSIIIQKDDSFPIRSYIAETIQGKKLWRIFLFAALILLASEVILLRFWK"
     gene            complement(4882..5562)
                     /locus_tag="PLF20_07775"
     CDS             complement(4882..5562)
                     /locus_tag="PLF20_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOX80934.1"
                     /translation="MRLKINQITFLFLIIVVFSLENLHAQEKLRIGLLKYSGGGDWYA
                     NPTSLPNLIKFCNENLHTNIDPEPATVEPGDPSLFNYPFVHMTGHGNVIFSEQEISNL
                     KKYFEAGGFLHIDDNYGMDLYIRRELKKIFPDQDFVELGPNHPIFHQRYDFPNGLPKI
                     HEHDNKPPQAFAFFLNGRMVCLYTYECDLGDGWEDIRVHGDSEATHLKALQMGANLIQ
                     YVFTQVIQ"
     gene            complement(5567..6289)
                     /locus_tag="PLF20_07780"
     CDS             complement(5567..6289)
                     /locus_tag="PLF20_07780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOX80935.1"
                     /translation="MQVFYSDQLSPAMVLPARIKLNDEESHHAIKSMRLKNGDSLILT
                     DGKGYHYKAMMAASAIPNAEVIVNEVRQVKDFDKPHLHLAISLLKNPDRLEWMLEKAV
                     ELEIDEISFIICERTEKPSVNMERMKRIAIAALKQSMGACLPNIYGPLLFDEFLENYN
                     PRWRGFIAWCEERNNILWEVCPQNTDIVVIIGPEGDFSNREVNKAISKGFVPVNLGRK
                     RLRAETAGLTSIMWFHLFNKIY"
     gene            complement(6292..6990)
                     /locus_tag="PLF20_07785"
     CDS             complement(6292..6990)
                     /locus_tag="PLF20_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HOX80936.1"
                     /translation="MKRYKDLFFDLDDTLWDFDRNSEETLRKLYHGYLAQELGKENYA
                     RFLSTYHRINERLWNDYKLNKIKKSEVAYQRFLFTLDESGLSNASLARLLAEEYLRSS
                     PAKTNLIAGAVEVLETLAKSYKLHIITNGFTEVQIPKLHNSGIRHYFTTLTTSEEAGC
                     LKPCGQIFNFALEKAEAQINESLMIGDDPEGDIRGAREIGLDTVLFVKHHKRIQTEST
                     YTISELKELLQILS"
BASE COUNT         2157 a         1499 c         1501 g         2413 t
ORIGIN      
        1 gttcacatat aatttttcgt tggttgcagc tactgctgct gcttctattg tatcgaaact
       61 gcgtagttcg gtttttcgtt cattatggat cccgtattta ttgccaatat gttcgaacca
      121 ttcgatggca tgatctatca aattctcaag ccgctctttt actttttgca tctcattttc
      181 aatactttca atatgacgat cagcctcaaa agtattgtag gccgagatac gttttattct
      241 tatttcagta agatgagtga tatcttcgta ggtaacttcg cgatgcaaga gttgaaggaa
      301 gggtttcaat cctttttgaa tggtactgat ggcttcttcg aaacttttac tttgcctgat
      361 ctggagataa atttcttttt cgatgaatat tttttccaat gaactgtggt gccagtcgtc
      421 ttccagctcg ttaagcctga ttagtaattc tttcttcagt aattcgacgg tattctgagt
      481 attgatacgt aagatttcgt taacgtcgat gaaggcaggt tttccattca gaatgacggt
      541 agcattaggg cttatattta cttcgcaatc ggtaaaagca tagagggcgt cgatggtttg
      601 gtcgggtgat acgccggaag cgatgtgtac aagaatttct acgttttcgg ctgtattgtc
      661 ttcaattttt ctgattttta tttttccttt gtcgttggca gtcaaaatgc tgtcgatgag
      721 gctggaggtg gtaactccat aggggatttc ggttatggca agggtttttt tatcgagttg
      781 aacgattctt gcacgtactc ttatcttacc acctctgaga cctcggttgt atttagaaca
      841 atcggccagt cctcccgttt ggaagtcagg gaaaatttcg aagtcttcat tatttaggat
      901 ctttatgctg gctttgatca gttcgttgaa attatggggt agaattttag aagcaagtcc
      961 cacggcaatt ccttcaacac cttgagcaag cagaagaggg aattttacag gaagggtaac
     1021 aggttcttta ttacgaccgt cgtaactgag tttccattga gtcgttttag gattgaatac
     1081 gacttcgagg gaaaattttg agagacgcgc ttcgatgtat cgtggtgcgg cagcctgatc
     1141 acccgtgaaa atatttcccc aattcccttg acaatcgatt aaaagctctt tttgccccag
     1201 ttgtatcagt gcgtcgccaa tagattgatc gccatgagga tgatatttca tggtttggcc
     1261 tatgatgttg gccaccttat tatagcgtcc gtcgtccatt tcgtacatgg catggagaag
     1321 gcggcgttgt accggtttaa ggccatcggc catctcgggg atggctcttt ccatgatcac
     1381 ataggtggcg taatcgagaa accagttctc gtacattccc ctcaaagtaa ttgttctata
     1441 ctctgagctt gctgaggttt cattttcctt gatggaatcc tcgggaaata attcttcgtt
     1501 ttgaatattt tcgtccatgc cttacacccc gtccgtaaaa gtaggatggg tcaaatcatt
     1561 tccggtttat tgttttcact gaacgacgaa tcgttttctt ttaatcttct ggccatttgt
     1621 ttacccagtt ctatcaatga tacaatatct ttgtcacttg ggctgtgctt aacttcgata
     1681 gcctcgccaa tcagttccca tttgatattt tcggcaaatt tttttaaagt acttactcca
     1741 ccgcctcccc agctcatcga accaaaaata gatagaaagt gattcctggg cataatatgt
     1801 tccaattgca tcaggaggtc gcgcatggga gtaaatattc caccattata tgcacagctg
     1861 cccagaataa cccctcgata tttccatata tcgctaatga tataactgct atgcgttttc
     1921 gatacatcgt aaattctgac attgcgaata tcttcttcgg ctattgctct cgcaataata
     1981 tcggccatct tttcagtatt gccatacatc gaaccaaatg ctatcactac tccttgttca
     2041 gttttataag tactccagtt tctataaaat tccacaactt tcggaatatg taaacgccag
     2101 atagggccat gagtcgaagc gatcatgcgc aagtcgagcg actcgagttt agcgagggct
     2161 ttctgtacct gtaatccata tttacccaca atattcgaat aatatcttct catttcgtct
     2221 tcataaaacg aaagattcaa ttcatcatca aatatgcctc cgtccaaggt accatagctt
     2281 ccgaaagcat cggccgaaaa aagtattttc tctgtcgatt cataagtcat catggtttcg
     2341 ggccaatgaa ccatgggtgt taaatagaaa tgaaactgat gttgacctgt atgtaggacc
     2401 gcattttcct ccacttccat caaatttttt ttaataccat aataaccctc gagcatagga
     2461 aaggttcgat tgttgccaac aatggttact tgaggaaatt cattgataat ggtttttaca
     2521 gcgcccgaat ggtctggctc catgtgattg ataatcaaat aatttattgc acggccgttg
     2581 agaatttctt tgagtttctc gagaaaagaa tccattgtgc ccattgaaac cgtgtccatc
     2641 aatgtaatct ggtcgtcgag aatcaaatac gaattgtaag ctattcccga aggaataggc
     2701 caataattct cgaataagcg agttttgcgg tcattggtgc ccacccagta aatgttttct
     2761 gacagtcgaa ttttaggata tatcattatt gtaaaacttc gttatttcca aaaccgcagc
     2821 aaaattactt ctgatgcaag taaaatcaaa gctgcaaaaa ggaaaattct ccagagtttt
     2881 ttcccttgaa tggtttcggc aatataagat cggataggga aagaatcatc cttttgtatg
     2941 attattgagt tggtccagcc cttttgctcg agcattctat cgagttcgtc gaccgaaaaa
     3001 caagtgggca aggattccgt gcgtggggca ttcaaagcga taaaaccatt cgttttattg
     3061 ttccttctta aatgataaat acccggttcg ggaagttcat tgcggacaat aatctcagtt
     3121 tggccatctc tatctctgaa aaatgggata atatcgatag tattgtcgct ttttatcaga
     3181 tgaggtgctg gagtagtttc ggcaggtttc tcgaaataaa aaatggatgg tttgccgtaa
     3241 acagcataca agggattttg atattgacca ctaatagcca tgtttaaata agcagctacg
     3301 aatagattgt tgcgcatgaa atctgtaaat tcaggacgaa ggggggaagc ctgaaaaaat
     3361 aaatgaccat ttcccaaacg tttggcaatg agaaagggat cgccatttcg taaagtcatc
     3421 aaattatcat agcccgatcg ggaattcaaa tttattttaa aatatttttt aactgacggc
     3481 aacaaaggca tattaggcat tctttcgata gctggtttaa aaataaaatg gtcagtattt
     3541 acctgtgaaa ctgtagtcga tgaagtatcg gcgtgcaatt ccagccctgc cccaagatta
     3601 gataagaatc tttctaaatg atcaatccct ttggagggat ggggtagaat agccacggag
     3661 tgccctgcat tgagactgtg tcttacgctc aatatcagac cctgaggata ttcggtcaac
     3721 tcgttcaaaa tggttagaga ggatttttcg agccgggaat aaataagctg atgaataggc
     3781 tgattatcaa cccacatcaa actgtcggaa tgaaataact gattgagatg aggattttct
     3841 ccttcactat tattgacaat aaggatttcg agctttggat gaacagtata ggcaaaataa
     3901 agctcatcgt cgaaagtaaa accattatcg ttaatttgta gcgatcccat tatagtacca
     3961 gtatcggcat taaacagcgg gaatgaaaga gtgaaagtag ttttgggagt caagcttaag
     4021 ggtttgagtc cgcgttgtat ttcatttgtg ctgagtttta ccatcagctc acggctttgg
     4081 ttggcactat gattcgtaac attgacacga agcacggatt cgagaccggg agcaataatt
     4141 ggtgtatcga accagcaagt atcaatactg acatttaagg gaagaggcga ttgcatgggg
     4201 ataaaaatcc aagtaatatt cgtatcaaga ttagttggta aactaaaagt gcttctggca
     4261 aaatccgaaa taaaaaaagc caaatgattg tagttttttt catagcgaag caagttagcc
     4321 tgagtttgca ataaagtaga agtgcttacc tgtgccagac tcgatttaac atcatttatc
     4381 cagatactcg cctcattggc aggtacaaaa tgctgatatt tcccttccaa atcgttggta
     4441 agcacttgaa acttgtcggt cgaagaaaaa ctgttcacga tttcgagggc cttttctctg
     4501 gcctggtgca acagattttc attacccata cctgtttcca tgctctgtga gttatctaaa
     4561 taaatgctca cggcaaaggg attcccctgc tttatattgt tggaagccgg aatataaggc
     4621 tgggcaaatg ctaacaccaa ggtaatgatg aaaagtattc gtaagagcaa caaaatcaaa
     4681 tttcgaagac gcgacgatct atgcgtctct gattggatag cctgtaaaaa ccttacattg
     4741 gtaaaatata cttttttata ccgatgaaaa ttgaaaagat gaataacgat agggattgaa
     4801 atagcggcca atccgactaa ataccaagga tagataaatt ccatgattga atataaacaa
     4861 aatcaattga aacgtaaaaa ttcattgaat tacctgagta aacacatatt gaatcaaatt
     4921 tgctcccatt tgcaaggcct tgaggtgagt agcctcgctg tcgccatgca cgcggatatc
     4981 ttcccagcca tcgcccagat cgcattcgta agtatacagg caaaccatcc tgccattcaa
     5041 aaaaaaggca aaagcctgag gtggtttgtt gtcgtgctca tgtattttgg gcaagccatt
     5101 cggaaaatca tatctttgat gaaatatggg gtgattgggc cctaattcca caaaatcctg
     5161 atcggggaag atttttttta attctcgtct gatgtatagg tccattccat aattatcatc
     5221 tatatgaaga aatcctccgg cttcgaaata ttttttcaga ttactgattt cctgctcact
     5281 gaatatcaca ttcccatgtc ccgtcatgtg gacaaaggga taattaaaca gcgagggatc
     5341 gccgggttca acggtggcag gctcggggtc gatattcgta tgtaaattct cgttgcaaaa
     5401 tttgatcagg ttaggtaaag aagttggatt ggcataccaa tcgccacccc cactgtattt
     5461 caaaagtcct atcctcagct tttcctgtgc atgaagattt tccaaagaaa acaccacaat
     5521 tattaaaaac aaaaaagtta tctgattaat ttttaatctc atactattaa taaattttgt
     5581 tgaatagatg aaaccacatg atggaagtca aaccggcagt ttcggctctc agccgcttac
     5641 gacccaagtt tacaggtaca aatcccttac taattgcttt attgacttct ctattagaaa
     5701 aatctccttc gggaccaatt attacgacaa tatctgtatt ttgagggcaa acttcccata
     5761 aaatgttatt tctctcttcg caccatgcga tgaaacctct ccagcgaggg ttgtaatttt
     5821 ccaaaaattc gtcgaagagc aatggcccat atatatttgg taagcatgct cccatcgatt
     5881 gttttaaagc cgcaattgct atgcgtttca tcctttccat attcacgctt ggtttttccg
     5941 ttcgctcgca aataataaat gatatttcat ctatctccaa ttccacggcc ttttcgagca
     6001 tccattccag acggtcgggg tttttgagca aagaaatagc caaatgaagg tgaggtttgt
     6061 caaagtcctt cacctgccta acttcattca ctatgacctc ggcattggga atagcacttg
     6121 cagccatcat tgccttatag tgatagcctt tcccatcggt cagtatcaaa gaatcaccat
     6181 ttttcagacg catcgacttg atagcatgat ggctttcttc atcgttcaat ttgattcttg
     6241 ctggaagaac catagctgga cttaactgat cggagtaaaa tacctgcatg cttaacttaa
     6301 aatttggagt aattctttta attcggaaat cgtataggtc gattccgttt gtattctttt
     6361 atggtgtttt acgaacaaaa ccgtatcgag accaatttct cttgctcccc gaatatcacc
     6421 ttctgggtca tcgccaatca tcagactttc attgatttgg gcctctgctt tctcgagagc
     6481 aaagttaaaa atttggccgc agggtttcag acaacctgcc tcctcagaag tagtaagcgt
     6541 tgtaaaataa tgtcttatgc ctgaattgtg caatttgggg atttgtactt cagtaaagcc
     6601 attggtaata atgtgtagtt tataactttt ggccaaggtt tccaaaactt ctacagcccc
     6661 tgctatcaaa ttggttttgg ccggacttga acgcaagtat tcttctgcaa gtaatcgagc
     6721 cagcgaagca ttgcttaagc ccgattcatc taacgtaaac aaaaaccgct gataagcaac
     6781 ttccgatttt tttatcttgt tcagtttata atcattccat aacctttcgt tgatacgatg
     6841 ataggtcgag aggaatcgtg cgtaattttc ctttcccaat tcctgagcca aatatccatg
     6901 atataatttt cttaaggttt cttccgaatt acgatcgaaa tcccacaagg tatcgtcgag
     6961 atcgaaaaaa aggtctttgt atcgcttcat ctgtaaaggt caataaaatg atttgcaaaa
     7021 taaacacatt ttgactcttc cgtgcttgaa agtggcattt catcatcgag aatcgaagac
     7081 caaatcatct cttatctcaa gtatcaagca tatcttatta taagtattca ggaggctgtt
     7141 tatctttatc tggttcagcg gtttgaaaaa tgatttagcc tgatttacag ttattcgaat
     7201 ttcatcctaa aaaattttgc ccaatcccca gcttatctat ttgttataac ttattttctg
     7261 gataggtaga atttctgttt gctttttgta atggattcct tcttcaaagg gaaaaattta
     7321 actggtttta tgaaattgtg ctcataaaag tattatattt gaacttgatg gggataaatg
     7381 tattccacca atgtatttaa cttaaattca attatttata tgaagaaaaa cgtagttggt
     7441 ttaatttttt gtttgctttg gggagcgatg tatcccttag cagggcaaat cgtttgggtt
     7501 cctttcgatg agtcgaacgc agcaaatcag cttacacctt cccactcatc tcattcccgg
     7561 catttatctc
//
LOCUS       DAOORU010000119         7530 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_12068_length_7530_cov_4.885619, whole genome shotgun sequence.
ACCESSION   DAOORU010000119 DAOORU010000000
VERSION     DAOORU010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7530
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12068_length_7530_cov_4.885619"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(206..820)
                     /locus_tag="PLF20_07790"
     CDS             complement(206..820)
                     /locus_tag="PLF20_07790"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOX80937.1"
                     /translation="MNTILLCLTCLFVGYLIGSILFAHLITRIISGKNIRELGNLNPG
                     AYNVFTQVNKFWGVVSGLLDAVKALLPMMIASYFFDVPNIALGCLGIGAIFGHRYPLY
                     YHFKGGRGASTLLGMFLFFIPIEFGISLVVDAILVLGFIRKQYGTWGPSILIALSLVS
                     CLFFQHPAEVKILVWIGGLIVLWFNRDGLQKLFIKPVAEETLSN"
     gene            complement(832..1716)
                     /locus_tag="PLF20_07795"
     CDS             complement(832..1716)
                     /locus_tag="PLF20_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOX80938.1"
                     /translation="MEKKIFFITADNLGLTREQIDYPHLEILKFPVIIDGIGYRQSDE
                     YNAKWLISKYENENIVAKSSTLVQKEIVDIVEKYHNDFDMMIHVLMSSGMSAASFKVA
                     ENVRKQFENVIPIINIDSRQAINGVGNVLLAIIDIMKANPILSPEEIDQKAQEVVEST
                     FSYFVLPDLKYLYKGGRVGKAQSLMGSILHIIPVVGLIGTEKEGIIVPIGKGRTFKQV
                     NSLIYDKITEKMNEKGVSKIKRIISIFGYGDKNADAFTDLKEKVQAIPCDDFIEGKPA
                     IVDAVYCGPGAYGVSVYL"
     gene            complement(1727..3541)
                     /locus_tag="PLF20_07800"
     CDS             complement(1727..3541)
                     /locus_tag="PLF20_07800"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HOX80939.1"
                     /translation="MNKRDATLSISNTEDLAEVINDIHHYLNKHKEYLNSLNVFPVPD
                     GDTGLNMVLTLQGAMANMTGKASLEKSAGEYLKEFAEHMLLNSRGCSGVILSLFVQGF
                     ANVVSNNNFSKENIYKAIENGYRNAYEGTKNPQEGTMLTLMRAFRDKFYEQMQHEDNP
                     LLIIRDIIPQLKEVLNQTPEMLPILKQAGVVDSGAAGFIILLEGIDKELKNHQLTGIS
                     IPSVLGIGKTIRKLLKDRRSNLKRDPLLPLILNIDFNRIYNKRLQEVLADARKVFGHF
                     HINNRMSLQKKIIDNLQGIDKSWNPEIKYRYCTEFTLESDHISKEHLIELLNGYGDSL
                     IIIQSNNRFKVHIHTNKPNDVFEQASEYGNLLFTKVDDMKKQHRNFISEDIIGFERDK
                     SVFCIVSGRGFAEILKNLGADDILCYGKNKPSVDQLLKNLNNLKAKNIIAAADDHDIL
                     MALKLAISLCKSNVYVVESDNPISLISIMMNMPKDYDITTMFEEAMKNLHNIPFSSIA
                     RATRDVSGINGIEVKKKDYFAIYNKSIITSNPRLNSLIEEIIGRFAADSVLITFYRGE
                     KVKNNAELLQVLKIKYQTIEFEEYYGGQSKYDYYITFE"
     gene            complement(4193..5239)
                     /gene="rlmN"
                     /locus_tag="PLF20_07805"
     CDS             complement(4193..5239)
                     /gene="rlmN"
                     /locus_tag="PLF20_07805"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOX80940.1"
                     /translation="MQRLYGKTLAELGEIAQALSFPSYTAKQIAQWLYQHHINHIDEM
                     NNLSKTARDKLKERFTLGLSAPVRVQISKDGTKKYLYSLAGGRFIEAAYIPDNDRGTL
                     CVSSQMGCKMGCLFCMTGKQGFQANLTVNEILNQIRCLPEWHELTNIVFMGMGEPLDN
                     IDNLMKSLEILTSDWGYARSPKRITVSTIGIIPTMKTFLENSHCHLAVSLHSPFNEER
                     KKLMPVENVYPINEIVQLLKNYDWTGQRRLSFEYIMFKGLNDSLTHARALLKLLNGLN
                     CRLNLIRFHAIPGSTLEPSTNETIHSFKDFLNSKNLLTTIRASRGEDIFAACGLLSTK
                     ELIQQKNIGNSIGD"
     gene            complement(5475..6848)
                     /locus_tag="PLF20_07810"
     CDS             complement(5475..6848)
                     /locus_tag="PLF20_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80941.1"
                     /translation="MGSLAMDSFGNIALGYSVSSSTVYPGIRYTGRLVNDPLGEMTIQ
                     ETTIIDGGGSQTGGFMGNGRWGDYSAMMVDPVNPSTFWYTQEYMQTTSSQGWKTRIAS
                     FSFENVFAVTLTASPDHICLGDSSHLFALAMGGTGNYTYSWTSYPEGFISNLPNPWVS
                     SIDTTIYICQVTSGNDTITDSVSVFVNLPPVVWAGNDTVICANNSLQLDALAVNYDSL
                     LWITSGDGTFSANNTLTSVYTPGAGDIASGSVNLGLYATALTGCPGPAFDEIILNITP
                     LPVVSAGTDTLICFWQLPFGCMGQSTNTTSIYWTTYGDGTFDDPENLNAKYFAGSNDM
                     NNGSVILKLTGQPLIPCTDNVSDEMTLYLDPCVGIVENNSPNFQLHIHPNPNNGNFFV
                     TLNASKSAEIVLCITDNKGNEIYRNTLSSSKIQHYPINLGNPSSGVYLLKVESKTGKS
                     TAKIIVK"
     gene            complement(6939..>7530)
                     /locus_tag="PLF20_07815"
     CDS             complement(6939..>7530)
                     /locus_tag="PLF20_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80942.1"
                     /translation="KDNWELIAISTSPDPTGSWYRYAFQFSDMPDYPKLGVWNDAYLL
                     TVNRFSSGSTTYKGIGAYALQRSKMLVGDPNATMITFTYGTNGPYSMLPADVDGTLPP
                     EDEPAYFAYKSDPNQLVIYKMNIDWTNPTASTFGLDQSILVAFYSSNISGIPQPGTTR
                     KLDPITDRLMFRLQYRNFGSYASMVTSHTVNVSGTA"
BASE COUNT         2262 a         1451 c         1455 g         2362 t
ORIGIN      
        1 tatctcatta tataattgtt acaaaaataa cctctaaaat gtgtcgaagt caggagatca
       61 atgcggctta tgacgatatc aagaaacaaa gaggatttaa ttgagtatca ccctcctcac
      121 gaggattaat tcattcctcc ttgtatagtt ttaattggat cgattcctgg gaatgaaatc
      181 caaaattatc ctcaacttta tcggtttagt tagataaggt ttcctcagct acaggtttaa
      241 tgaaaagttt ttgcaatcca tccctgttaa accataatac aatgagtcct ccaatccata
      301 ctaaaatttt tacctcggca ggatgttgga agaaaaggca agaaaccaag cttagcgcta
      361 taagtataga aggcccccat gtgccatatt gttttcttat aaaacctaaa accagtatgg
      421 catctacgac caaagaaata ccaaactcga tgggaataaa aaatagaaac atccctagta
      481 aagttgacgc tccacgccca cctttgaaat gataataaag agggtaacga tgtccgaata
      541 tggcacctat tcctaagcaa ccaagagcga tgttgggaac atcgaaaaaa taactggcaa
      601 tcatcatggg caacagtgcc ttaacagcat ccaataatcc tgacaccaca ccccagaatt
      661 tattcacctg ggtaaaaaca ttataagcac ctggattgag atttcccagt tctctgatgt
      721 tttttccaga aataattctt gtaatcaaat gggcaaaaag aatggagcct atcaggtatc
      781 caacaaaaag gcaagtcagg cataatagaa tcgtattcat aacaaaaaag tttacaaata
      841 gactgaaact ccataagcgc ctggtccaca gtaaacagca tctacaatcg ccggtttccc
      901 ctctataaaa tcatcgcagg gaattgcttg caccttctct tttaaatcgg taaaggcatc
      961 ggcattttta tcgccataac caaaaatcga aatgattctt tttattttac ttacgccctt
     1021 ttcgttcatc ttttcggtta ttttatcata aatcaaagaa ttgacttgct taaaggttct
     1081 tcctttgcca ataggcacaa ttatgccttc tttttcagtt cctattagcc caacaacggg
     1141 tataatatgc aaaatacttc ccatcaatga ttgggccttc cctacccttc ctcctttata
     1201 caaatatttc agatcgggta aaacaaaata actgaaggtg ctttccacta cttcctgggc
     1261 cttttgatcg atttcttccg gactcaaaat gggattggct ttcatgatgt cgatgattgc
     1321 taacagaaca ttccccacac cattgatggc ttgccggctg tcaatattta ttattgggat
     1381 cacgttttcg aactgtttac gaacgttttc ggccacttta aacgaagccg ccgacattcc
     1441 gctactcatc agcacatgaa tcatcatatc gaagtcatta tggtatttct ccacaatgtc
     1501 tacaatttct ttttgaacta aagtggatga tttagctacg atattctcat tttcatattt
     1561 tgaaatcaac catttagcat tatactcatc gctctggcga tatcctatcc catcgatgat
     1621 aaccggaaat ttcagaattt caagatgagg ataatcgatt tgttctctgg taagccctaa
     1681 attatcggct gtaataaaaa atattttctt ttccataacg gtgattttat tcgaaagtta
     1741 tataataatc atatttcgac tggcctccat aatattcttc gaattcgata gtttgatatt
     1801 tgatttttaa aacttgcagt aattcggcat tgtttttaac tttttcccct ctataaaagg
     1861 taattagaac actatcagcg gcaaatctgc caattatttc ctcgatgagt gagtttaatc
     1921 gagggttgga ggttattatt gatttattgt atattgcaaa ataatccttt ttcttgactt
     1981 ctatgccatt tatgccagat acatctcttg tggctcgagc tatggaagaa aaggggatgt
     2041 tgtgaaggtt tttcattgct tcttcgaaca tggtagtaat atcgtaatct ttcggcatat
     2101 tcatcattat gctgatcaaa gaaatgggat tatccgattc gaccacatac acattcgatt
     2161 tgcataacga aatagctaat ttcaaagcca tcaggatatc atgatcgtcg gccgcagcaa
     2221 ttatattctt tgctttcagg ttattcagat tcttcaaaag ctgatccacc gagggtttat
     2281 tcttgccata acagagaata tcgtcggctc ccaaattttt taatatctca gcaaatcctc
     2341 ttcccgaaac aatacagaaa acacttttat cgcgttcgaa tccaataata tcctcactaa
     2401 tgaaatttct gtgctgtttc ttcatatcat ctacttttgt aaacagcaag ttgccatact
     2461 ccgatgcttg ttcgaaaaca tcattgggtt tattggtatg aatatggacc ttgaatctat
     2521 tgttgctctg aattattatc aaactatcac cataaccgtt caataattct atgagatgct
     2581 ctttcgaaat gtggtcggat tccaaggtaa attctgtgca atatctatat ttgatttctg
     2641 gattccacga tttatcgatt ccctgcaaat tgtcgataat ttttttctgg agagacattc
     2701 tattgttgat atgaaaatga ccgaaaactt ttctggcatc agccaatact tcctgtaacc
     2761 gtttattgta tatgcggttg aaatcgatat tcaaaatcaa cggaagtagg ggatctctct
     2821 tcaaattcga tcttcggtcc tttagcaatt ttcttatggt tttaccaata cccagtaccg
     2881 aggggataga gatcccggta agttgatggt ttttcagttc tttatcaata ccttccaaca
     2941 atataataaa acctgcagca cccgagtcga ctactcctgc ttgtttcaaa attggcagca
     3001 tctcaggagt ttgattcaaa acttctttca attgagggat aatatcccgt ataattagaa
     3061 gtggattatc ttcgtgctgc atttgttcat aaaacttatc cctgaatgcc ctcatcaggg
     3121 ttagcatagt tccttcctga ggatttttgg tgccttcgta agcatttcta tacccatttt
     3181 caatagcctt gtagatattt tccttcgaaa aattattgtt gcttaccaca tttgcaaagc
     3241 cctgcacgaa aagcgaaaga ataacaccag agcaacccct gctgttcaga agcatgtgct
     3301 cagcaaactc cttcagatat tctcctgccg atttttctaa agaggctttg cctgtcatat
     3361 tcgccattgc tccctgaagc gtgagaacca tgttaagtcc cgtatctcca tcaggtaccg
     3421 gaaatacatt caaactgttc aaatattcct tgtgcttatt gaggtaatga tgaatatcat
     3481 tgatcacctc tgccaaatct tctgtatttg agatagataa agttgcatct cttttattca
     3541 tatctttcaa attataaaaa ttcaggtcaa gtggctcata ttcatttaaa ttgtttctaa
     3601 agatacaaag ttaggagaat gttcttaagt tttacaaatt cctatattga aaccttagag
     3661 aaaataaatt acaattggct ttatctcatt gattgcgaat aacaaatcga tagaattaga
     3721 attattcgaa tcaaattaaa tcaatatcag cttgtaagga gaattaatga atcaaagact
     3781 gatatgttgc aaagaggata acatatttta tattactcta tcctctattc gaaaatatta
     3841 aagaggagtt ggaagtttat cttattgttt tatttttctt tttctctttt tatccaagcc
     3901 tgcttttgat tgatatggtg aaatagctct atgaagcctt tcagatcttt tttcggagaa
     3961 tcgaatatga atggagagat gaccgagaat ttggttttta atgatatggc tcatcagttt
     4021 caatccttag ggttccaatg atgaaagtat agttataagg agaaggattt agagatagat
     4081 atgatatttt tcaatgctca ttcaatagaa agatctcttg aatggggtgt gtaatagtta
     4141 tgaatggttt tgaaacgact ttataaaaag gcagggcgtt ccccatcatt tcttaatctc
     4201 cgatcgaatt tcctatattt ttttgctgga taagttcttt tgtgctaaga agcccgcagg
     4261 ctgcaaaaat gtcttctcct ctcgaagctc ttatggtggt aagaagattt ttggaattta
     4321 gaaagtcttt gaaggaatga atagtttcgt tggtcgaagg ttctagggtt gaaccgggga
     4381 tggcatgaaa gcggatgaga ttcaaacggc aattcagtcc attgagtaat tttaacaggg
     4441 ctctggcatg agtaagtgaa tcgtttaatc ctttgaacat gatatattcg aatgacagtc
     4501 tgcgctgacc tgtccaatcg taatttttga gaagttgaac gatttcattg atagggtaga
     4561 cattttcgac aggcatgagt tttttacgtt cttcattgaa aggagaatgg aggctgacag
     4621 ctagatggca gtggctgttt tcgaggaaag ttttcatggt agggattatt cctattgtcg
     4681 aaacggtgat acgcttcggg cttctggcat aaccccagtc ggaggtaagt atttcaagac
     4741 ttttcattaa attatctata ttatcgagag gttcgcccat tcccatgaac acaatattgg
     4801 tcagttcatg ccattcgggt aggcatctaa tttgattcag gatttcattt acagttaggt
     4861 ttgcctgaaa accttgtttt cccgtcatgc agaataaaca acccatcttg caacccatct
     4921 gagaggaaac gcaaagcgta ccacggtcat tatcgggaat ataagctgct tcaataaatc
     4981 gccctcctgc caagctatac aagtattttt tggtcccatc cttcgaaatt tgaactcgta
     5041 caggagccga gagtcccaaa gtaaaccttt cctttaattt atcacgagcg gttttcgaaa
     5101 gattattcat ctcgtcgatg tggtttatgt gatgttgata aagccattgg gctatttgtt
     5161 ttgctgtgta ggaaggaaag ctcaatgctt gggcaatttc gcccaattcg gccaatgttt
     5221 ttccataaag gcgttgcata tcctggagtg aataaatata tttccgagct gggttattta
     5281 aatttagggc ttttgcaatg aattagggta tcttgtgctg caaaaatcca tttcggtaat
     5341 attcgcaaaa ataagtaata aatttatttg tatgtagaaa ctcctaaggg ttgaatgaac
     5401 ataaattata ttctaaacgt gaagcagcct catcaattaa aaactgacga ggctgcattg
     5461 aaaagaatgg ttatttattt aactataatt ttggctgtgg acttgccagt tttactttca
     5521 accttcaaca aataaacacc cgaagatgga tttccaagat ttataggata atgttgaatc
     5581 ttagaagaag acaaggtatt acgataaatc tcattccctt tattgtcggt aatacataaa
     5641 acgatctcgg cagattttga tgcattcaag gtaacaaaga aatttccatt gttagggttg
     5701 ggatgtatat gtaattggaa attgggagaa ttattttcga ctatacccac gcaagggtcg
     5761 agataaaggg tcatttcatc ggatacattg tcagtacaag gaatcagggg ttgaccagta
     5821 agttttagga taacgcttcc attgttcata tcattagagc cggcaaaata tttggcattc
     5881 aagttttcgg gatcatcaaa ggttccatct ccataagtag tccaataaat cgaggtggtg
     5941 ttggtggatt gacccataca gccaaaagga agttgccaga agcagatgag agtatcggtt
     6001 cctgctgaaa caacaggcaa aggagtaata ttcaggatta tttcatcgaa agctggaccg
     6061 gggcagcctg taagagccgt tgcataaagg ccgagattaa cgcttccgct ggcaatatcg
     6121 cctgcaccgg gggtatagac agatgtaagt gtattattag ccgaaaaagt cccatctcca
     6181 gaggttatcc aaagcaggga atcgtagttt acggccaaag catcgagttg aagactgttg
     6241 ttagcgcata ttactgtatc gttcccggcc caaaccacag gtggaaggtt cacaaaaaca
     6301 gaaacactgt cggtgattgt atcatttcca cttgttacct ggcaaatata tattgttgtg
     6361 tcaatggaac ttacccaggg attaggaaga ttggaaatga atccttcggg ataagatgtc
     6421 catgaataag tataattacc cgttcctccc attgctaaag caaacaaatg agatgaatcg
     6481 cccaagcaga tatgatcggg ggaagcggtg agtgtaacgg caaatacatt ttcgaaggaa
     6541 aaggaggcaa tacgtgtttt ccagccttga gaactggttg tctgcatata ttcctgagtg
     6601 taccagaatg tagaaggatt aacgggatcg accatcatgg ctgagtaatc gccccaacgg
     6661 ccattcccca tgaatcctcc agtttgggaa cctccaccgt caatgattgt agtttcctga
     6721 atagtcattt ctcccaaagg atcattcacc aagcgtccag tataacgtat tcccggatag
     6781 acggtagaac tggatacaga ataacctaag gcaatgtttc cgaaagaatc catggcaaga
     6841 gagcccatcc agcgatgtac attgtcgggc gcataagttc cttgctgata gagcgaccat
     6901 gaggttgtgg gggtctttct tacttcatac catcttactc acgccgtacc acttacatta
     6961 accgtatggc tggtaaccat gctggcatac gatccaaaat ttctgtactg taacctaaac
     7021 atcaggcgat cggtaatagg gtcgagtttg cgagtggtac ctggctgtgg tattcccgaa
     7081 atattcgaac tataaaatgc aacaagtatg gattggtcta aaccaaaagt cgaggcagtt
     7141 ggattagtcc agtcaatatt cattttatag attactaact ggttgggatc gcttttataa
     7201 gcaaaatagg cgggttcatc ttcgggaggt aaagttccat caacgtcggc aggaagcatg
     7261 ctataagggc catttgttcc gtaagtgaag gtaatcattg ttgcattggg atcgcccacc
     7321 agcattttag accgttgaag tgcataagcc cctatgcctt tataagtagt gctccccgaa
     7381 gaaaaacgat tgactgttaa caaataagca tcgttccata ctccaagttt agggtaatcg
     7441 ggcatatcgg aaaactgaaa agcatagcga taccagcttc ctgttggatc aggcgaggtg
     7501 ctaatggcga taagttccca attgtccttt
//
LOCUS       DAOORU010000120         7487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_12159_length_7487_cov_5.381324, whole genome shotgun sequence.
ACCESSION   DAOORU010000120 DAOORU010000000
VERSION     DAOORU010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7487
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12159_length_7487_cov_5.381324"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="PLF20_07820"
     CDS             <1..261
                     /locus_tag="PLF20_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOX80943.1"
                     /translation="RRARFRTVISLIIQGKEYFFEGIVEGKIIENKRGNMGFGYDPIF
                     IPNGYSRTFAQMDEAEKNHISHRGIALRKMAEFLEYKKIFEQ"
     gene            complement(265..1248)
                     /locus_tag="PLF20_07825"
     CDS             complement(265..1248)
                     /locus_tag="PLF20_07825"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOX80944.1"
                     /translation="MPKLNIAVVGGGNSGEFDISIRSSKRVASLLDPLKYNVYLIVIK
                     GNNWEYINEKGRHYKINIQDFSLNIEGEQIVFDLVFNAIHGTPGEDGKIQGYFEMLGI
                     RHTSCNSITSALTFDKYFCNKVVSSFGVKTSPSVHLFRDKRISIEEIVKVLKLPVFVK
                     PSCGGSSVGMSKVTHCNDLKPAIELAFQHDREVLIEEFIPGREITCGVYSERGKSKAL
                     PLMEIVSKKEFFDYEAKYDPQLAEEILPAPIEENLAERCCSISEMLYSSLGCRGVVRF
                     DYILSNSEFWFLEVNTVPGMTAASIVPKMAIKAGFELGKFYDLLIEEALRD"
     gene            1486..2319
                     /locus_tag="PLF20_07830"
     CDS             1486..2319
                     /locus_tag="PLF20_07830"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOX80945.1"
                     /translation="MKFTDFIKSKLFFRNLALMVVVTLILIWLILQSLKLYTRHGQFI
                     LLPDFTGKPLDELVQFADEKGLKVIITDSIYDENMPPRSIRVMDPQPNTGVKSGRKIY
                     VTIASGTPDLVPMPDIRELSLRQAVETLVLSGLRIDYISYVEGPFQNSVIGQRIGTRN
                     VNPGELLPRNSKIVIVVEKGDNPVPAVVPDVIGLHPYEAYNTLFRSGLNLGKISFPDG
                     IPQDSAMVIHQEPGIETRLTPGSYVSLVFGQIKDAKQLKSLRQHHQNRDSIIDENEEE
                     F"
     gene            2337..4313
                     /locus_tag="PLF20_07835"
     CDS             2337..4313
                     /locus_tag="PLF20_07835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80946.1"
                     /translation="MKIDIEHWFLRSRMLKIIRNILLLLLCFLLKPLIAQEILSGLEY
                     NPAVGRESKVLLLKKTNQETSSLLLPFFDDFSKSEVFPDPNLWDDHNVYVNQGFAFNP
                     PNYRVVTLDALDSTGKVYAHATHTPFEADRFTSRPIRLDSIFSPAPRALRKADSIYLS
                     FYFQPQGHGNPPQKNDSLILYFYNSRDSIWNRIWATPGMSIDTFYAHHKSWWKQVNIW
                     IYDSAQYYHDGFRFRFVNYASLANNVLPSWQSSMDHWNLDYIYLNKNRYHSDTVYRDL
                     GFVNVSTSMLKTYNSMPYTHYSNDPVKEMNDTLYTVISNLDIVAHPSNYSFAVFDGNH
                     QQITSYSGGSYPVYPVYDSGYVKYKPFARPRITTILPIDPLGQKDSADFTVRHVIIGD
                     YTPEDRLFDTLEVKQIFRNYFAYDDGTPEAGYGVTPSGSMAAVRFSLNMKDTLRAVKI
                     YFNSTRSNANEQYFFLCVWDDNNGLPQNLLYDTIVKPDFAANINDFQTFIFEKPIPVR
                     NAFYVGFITTTDDNLNIGFDRSRNSSAMNLFNTGGQWLPSQFKGSLMIRPIVGKPLAG
                     VSKDKGNSKNLAVFPNPVIENVIYLSGELLLNGINENFTLSLFNSNGQLVFRSYLKPR
                     LDLPPSLSNGIYILQIRGKNGDIVYTTKLAVSKI"
     gene            4317..5348
                     /locus_tag="PLF20_07840"
     CDS             4317..5348
                     /locus_tag="PLF20_07840"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOX80947.1"
                     /translation="MNPEQDFNEDLFEHYRIVVDKGQSLLRIDKFLVKRVENASRTKI
                     QNAARAGNILVNHRTVKPNYKVKPGDVISILLPGEPREIENIPEDIPLNIVYEDEDVL
                     IVNKPSGMVVHPAYGNYTGTLFNALLFLFMGKTNKDGKEIYPWLVHRIDKDTSGLLII
                     AKNELAQAILAHQFYEHSIKREYTALIWGDLENETGTIEGYINRNPKDRRIMTIYQDP
                     EKGRTSITHYNVIKRWGYVTLINCSLETGRTHQIRAHFASIGHPLFGDTPYGGRQILK
                     GSLYSKYKQFVENSFGLLKGQALHARTIEFVHPSSHQKVYFESELPADFQEVIERWDK
                     YVLGRNLQS"
     gene            5457..7073
                     /locus_tag="PLF20_07845"
     CDS             5457..7073
                     /locus_tag="PLF20_07845"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOX80948.1"
                     /translation="MHTTRYIFVTGGVTSSLGKGIVSASLAKLLQARGYKVTIQKLDP
                     YINVDPGTLNPYEHGECYVTEDGAETDLDLGHYERFSNEPTSQANNVTTGRIYQAVIE
                     KERRGDYLGSTVQVIPHITDEIKYRIKLLSKYKDYDFIITEIGGTVGDIESLPYIETV
                     RQMRWEMGNLCVVIHLTLVPYLSSTGELKTKPTQHSVKTLLEQGVQPDIIVCRTEKPL
                     NEALRNKIALFCNVSSQAVIESIDMESIYEVPLKLRDEGLDREVLRKCGLPFEGQPDL
                     KSWEEFLYKMKNPKYSVEIGLVGKYVELKDSYKSIMESFVHAGTYNQVKVELRMIHSE
                     FLTQENLSEKLSGLHGILVAPGFGIRGIEGKILAIHYARTRGIPFLGICLGMQCAVIE
                     FAQNVMGLKKANSTEFDPRTPDPVIDIMEQQKKISGMGGTMRLGAYPCQLMEGSKSRN
                     IYKVPLILERHRHRYEFNNKYLEQFEQAGMIAAGINPKENLVEIVEIPKHPWFIGVQF
                     HPEFKSTVASPHPLFISFVEAACLHLEQNT"
     gene            complement(7296..>7487)
                     /locus_tag="PLF20_07850"
     CDS             complement(7296..>7487)
                     /locus_tag="PLF20_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80949.1"
                     /translation="ATAIVKVITRNPGVHPSRLTAAGRSEYAPLATNSTSEGRAKNRR
                     TEIILTPKLDELFQIIETH"
BASE COUNT         2267 a         1555 c         1498 g         2167 t
ORIGIN      
        1 agacgtgccc gatttcgtac ggtgatttct ctcatcatcc agggaaaaga atattttttc
       61 gaaggaatcg tagagggcaa aatcatcgaa aataaaaggg gtaatatggg ttttgggtac
      121 gatcctattt ttattccaaa tggttatagc cgaacttttg cccagatgga cgaagctgag
      181 aaaaatcaca taagccacag aggaattgct ttgagaaaaa tggccgaatt tcttgaatat
      241 aaaaagatat tcgaacaata aaattcaatc tcgcagagct tcctcgatta acaaatcata
      301 aaatttgccg agctcgaaac cagccttgat ggccatttta ggaactatac tggcggccgt
      361 cataccgggt actgtattta cttcgagaaa ccaaaactca ctgttcgata aaatataatc
      421 gaatcgaaca acccctctac aacccaggct cgagtaaagc atttccgata tcgagcaaca
      481 tctttctgca agattctcct ctatgggcgc aggtaaaatc tcctcggcca gttggggatc
      541 gtatttggcc tcataatcga aaaattcttt cttactcaca atttccataa ggggaagcgc
      601 tttcgactta cccctctcgc tataaactcc gcaggtgatt tctctgccag gaatgaattc
      661 ctcgatcagt acttccctgt cgtgctgaaa tgcaagttct atagctggtt tcaaatcatt
      721 gcagtgagtt actttactca ttccaacgct gctccctcca caactgggtt tcacaaaaac
      781 aggcaacttc aataccttaa ctatttcttc gatcgaaatc cttttgtctc gaaataaatg
      841 aacagaggga gaagttttta ccccaaaaga actcactacc ttgttgcaga agtatttgtc
      901 gaaagttaaa gccgatgtga tcgaattaca cgatgtgtgg cgtatgccca gcatctcgaa
      961 gtaaccctga atctttccgt cttcgcctgg agtaccatgt attgcattga ataccaaatc
     1021 gaatacaatt tgttcccctt caatattcaa gctgaagtcc tgtatattga ttttataatg
     1081 cctgcctttt tcatttatat attcccaatt attccctttg attacaatta gatatacatt
     1141 atatttcagg ggatcgagaa ggctagctac acgtttggag cttcgaatag aaatgtcgaa
     1201 ttctcctgaa ttgccccctc ccacaaccgc tatatttagt ttaggcattg ttctataaaa
     1261 tttagcatgc gaaattacaa aaacattgtt cagctatagg attagaaatt ttctttcgaa
     1321 cctaaagatc ataaagaatg aaacggccgg aaaaccttaa aaatgacaga ttattgatac
     1381 ttttgcaaac ctgagcaata aagtcattcg gataaagttt ctatgaaagt gaatgggaaa
     1441 taccctctta tatcgtccta tcaattataa taagaacatc ctgtgatgaa atttacggat
     1501 tttatcaaat ctaagttgtt tttccggaat ctggccttga tggtggtagt tacactgata
     1561 ctcatctggt tgattctgca atcgctgaag ttatacaccc gtcatggtca atttattcta
     1621 ttgcctgatt ttacgggtaa acctttggat gagctggttc aatttgccga tgagaaagga
     1681 ctcaaggtta ttattactga ctcgatatat gacgaaaaca tgccaccacg aagcattcgg
     1741 gtaatggatc ctcaaccgaa tacaggtgta aaatcaggcc gaaaaatata tgtaaccata
     1801 gcgtcaggta cacccgattt agttcccatg cccgatatca gagagctgtc attgcgtcaa
     1861 gccgtcgaaa cacttgttct ttctggttta cgaatagatt acatttccta tgtggaaggt
     1921 ccttttcaaa attcggtaat tggtcaacgt atcggcacgc gtaatgtaaa tccaggcgaa
     1981 ttgttgccaa gaaattccaa aatcgtcatt gtcgtcgaaa aaggggataa tccagtacct
     2041 gccgttgttc ccgatgttat cggcttacac ccttacgaag cctataatac tttgtttcgc
     2101 tcaggcctaa atctcggaaa aatttctttt cccgatggca ttccacagga ttcagccatg
     2161 gtaatacatc aggagccagg tattgagact cgacttactc ccggttcgta tgtaagcctg
     2221 gtttttggcc agataaaaga tgccaaacaa ctgaaatcac ttcgtcaaca tcatcaaaac
     2281 agagatagca tcattgatga aaacgaggaa gaattttaaa atttgaagtc aaaaaaatga
     2341 aaatagatat cgaacactgg tttttgcgaa gtcgaatgtt gaaaataatc agaaatattc
     2401 tgttgctctt attgtgtttt ttgttgaagc cattgatagc tcaggagatt ttatcgggct
     2461 tggaatataa tcctgctgtt ggcagagaat cgaaggtcct tcttttaaaa aaaaccaatc
     2521 aagaaacatc ctccttgctt ctgccttttt tcgatgattt tagtaagtcg gaagttttcc
     2581 ccgatcctaa tctgtgggat gaccacaatg tctatgtaaa tcagggcttt gctttcaatc
     2641 cacctaatta cagggtggtt acactggatg cattagattc tacgggaaag gtttatgctc
     2701 atgccaccca tactcctttc gaagccgatc gatttacttc gagaccgatt cgactcgatt
     2761 ctattttttc acccgctccg cgtgccttgc gtaaagccga ttccatttat ttaagcttct
     2821 actttcaacc tcaaggccat ggcaatcctc ctcaaaaaaa tgattcgtta attctctatt
     2881 tctacaattc ccgcgatagt atatggaacc gtatatgggc tacacctggc atgtccatcg
     2941 atactttcta tgcacatcat aaatcatggt ggaaacaggt aaatatctgg atctacgata
     3001 gcgctcagta ttaccatgat ggttttcgat tccgattcgt caattatgcc agcttggcca
     3061 ataatgttct gccttcatgg caaagcagta tggatcattg gaatctcgat tatatctatt
     3121 tgaacaagaa tcgctatcac agcgatacgg tttaccgtga cctgggcttt gtgaacgttt
     3181 cgacctctat gctcaaaacc tacaactcca tgccttatac ccattacagc aacgacccgg
     3241 tgaaagaaat gaacgatacc ctctataccg taatttcgaa cctcgatatt gtggcccatc
     3301 cttccaatta tagctttgcc gttttcgatg gaaatcatca gcaaatcacg agttattcgg
     3361 gaggaagtta tcctgtctat cctgtttacg attcgggtta cgtaaaatac aagccatttg
     3421 cacggccaag aatcacaact atacttccca tcgatcctct tggccaaaaa gattcagccg
     3481 attttaccgt caggcatgtg attataggag attatacccc cgaagaccgt cttttcgata
     3541 ccctggaagt aaaacagata tttagaaatt attttgctta cgacgacgga acccctgaag
     3601 ccggttatgg cgtcacacct tcgggttcga tggccgcagt gcgctttagc ctcaatatga
     3661 aagatacctt acgcgcagta aaaatctatt ttaattctac tcgcagtaat gccaacgaac
     3721 aatatttctt tttatgtgtt tgggacgaca acaatggcct gccacaaaac ctcctctacg
     3781 ataccatagt aaagcccgat tttgcagcca atattaatga tttccaaaca tttatcttcg
     3841 aaaaacccat tcctgtccga aatgctttct atgtaggttt tataactaca accgacgata
     3901 atctaaatat aggattcgac cgttcacgca atagctcggc tatgaacctg ttcaataccg
     3961 gcggacaatg gctgccctct cagtttaaag gttctctgat gattaggccc attgttggga
     4021 aaccacttgc aggtgtatca aaagataagg gaaattcaaa gaatcttgca gtatttccca
     4081 atccggtcat agaaaatgtg atttatttaa gtggtgagct gttgctaaac ggtataaatg
     4141 agaattttac cctcagtctg ttcaattcta acggacaact tgtttttcga agttacttga
     4201 aaccccgact cgacctccct ccttcccttt cgaatggtat atatattctt caaatcagag
     4261 gaaagaacgg ggacatcgtt tacacgacca aactggcagt ttctaaaatt taaaccatga
     4321 atcctgaaca ggattttaat gaggatttat tcgaacatta ccggatagtc gtcgataaag
     4381 gtcaatcctt attacgcata gataagttcc ttgtgaagag ggtggaaaat gcttcgcgga
     4441 ctaaaattca aaatgcagcc agggcaggga atattctggt aaaccataga accgtaaaac
     4501 ctaattacaa agtaaaacca ggcgatgtta tcagcatttt attaccgggc gaacctcgtg
     4561 aaatagagaa tattcctgaa gatatacctc tgaatattgt ttatgaagat gaagacgttt
     4621 tgatagtgaa caaaccttcg ggcatggtgg ttcatcctgc ctatggtaat tataccggaa
     4681 cgcttttcaa tgcgttgttg ttccttttta tgggaaaaac caataaagat ggaaaggaaa
     4741 tatacccatg gttggttcat cgtatcgata aagatacttc gggattattg atcattgcaa
     4801 agaatgaatt ggcacaagcc atacttgctc atcaattcta cgaacattcg atcaaaaggg
     4861 aatataccgc tttgatttgg ggagatctgg aaaatgaaac aggaacaata gaaggatata
     4921 tcaaccgcaa cccgaaagat cgtaggataa tgaccattta tcaggatcct gagaaaggaa
     4981 ggacaagtat cacgcattat aacgtcatca aaagatgggg ttatgtaact ttaataaatt
     5041 gcagtcttga aacaggccgt actcatcaga taagagccca ttttgcatct attgggcatc
     5101 ctctttttgg agatactccc tatggaggcc gccaaatcct caaaggttca ttgtattcaa
     5161 aatataaaca attcgtcgaa aatagtttcg gtttgttaaa ggggcaagcg cttcacgccc
     5221 gaaccataga atttgtccat ccctcaagcc atcaaaaggt ttatttcgaa tcggaattgc
     5281 ctgccgattt tcaggaagta atcgaaagat gggataaata tgtactggga agaaatcttc
     5341 agtcataata taattatttg aataataatg cgataactaa aattcaggga ttttcgaaaa
     5401 tctatttgaa atttgttgta acttgccgcc ccgtaacgta actaaaaaag ttaactatgc
     5461 atacaacacg atatatattc gttacgggag gtgttacctc ttctctggga aaaggaatag
     5521 tatcggcttc attggctaaa ttactgcagg caagaggtta taaagtaact attcagaaac
     5581 tcgaccccta tatcaatgtc gatccgggaa cactcaatcc gtatgaacat ggcgaatgtt
     5641 atgtaaccga ggacggggct gaaaccgatc tcgaccttgg ccattacgag cgtttttcga
     5701 acgaacccac ctctcaggca aataatgtga ccaccggaag aatctatcaa gcagtaatcg
     5761 aaaaggaaag acgaggagac tatttgggtt ctaccgtgca ggttatcccg catataaccg
     5821 acgaaattaa ataccgtatc aaactgcttt ccaaatataa ggattacgac ttcattatca
     5881 ctgagatagg aggtacggta ggcgacatag agtcgctgcc ttatatcgaa actgtacgtc
     5941 agatgcgctg ggaaatgggt aatctctgtg tagtgattca tcttacactg gttccttatc
     6001 tctcttcgac gggggaactg aagacaaaac ctacccaaca ttccgtaaag actttactcg
     6061 aacaaggagt ccaacccgac atcattgtct gccgtaccga aaaacctctc aatgaggctt
     6121 tgcgcaataa gattgcactg ttttgcaatg tttcttctca agccgtaatc gaatccatag
     6181 atatggaatc catttatgaa gtcccattga aattaagaga cgaaggcctc gacagagaag
     6241 tactgcgtaa atgtggactt ccgttcgagg ggcaacccga cctcaagagc tgggaagagt
     6301 ttctttataa gatgaagaac cccaaatatt ctgtggaaat tgggctggtt gggaaatacg
     6361 tagaattgaa agacagctat aaatctatca tggaatcgtt cgtgcatgcc ggaacttata
     6421 atcaggttaa ggttgaactg cgaatgatac attcggaatt tttgacccaa gaaaatcttt
     6481 cggaaaaact atcggggctt catggtatat tagttgcacc tggatttgga attcggggta
     6541 tcgagggtaa gattctggcc atccattatg ctcgaacaag aggaattccg tttttgggaa
     6601 tatgtctggg tatgcaatgt gctgtaatag agtttgccca aaatgtaatg ggactcaaga
     6661 aggccaattc taccgagttc gatccacgta cacctgaccc tgtgatcgac atcatggaac
     6721 agcagaaaaa gatttcgggt atgggtggaa ccatgcggtt aggggcttat ccctgccagt
     6781 tgatggaagg atcgaaatca agaaatattt ataaggttcc acttatcctc gagcgacata
     6841 ggcatcgtta cgagtttaat aataaatatc tcgaacagtt cgaacaagcc ggaatgatag
     6901 ccgccggcat caatcccaaa gagaatttgg ttgaaatcgt cgaaatccca aagcatccat
     6961 ggtttatagg cgttcaattt catccagaat ttaaaagtac agtggcttcg cctcatccat
     7021 tatttataag ctttgtagaa gctgcctgtc tgcacctcga acaaaacact tgataagtgt
     7081 tgtcaggata ttgggagatt cggtgcctga ggtacgaggg gacgagaaga taaggaaact
     7141 tttaagacag gtaattggaa agtcagctcg aataagattt tatgaaatat tttttaatgc
     7201 tcatatattc aatctcatcc tttcaacttt taacttttat ctcaccaatc ccttctcgcc
     7261 atccatcaga aaccttgaac tcctgaccac atgtgttagt gtgtttcgat aatctgaaag
     7321 agttcgtcga gttttggagt aagaatgatt tcggttcggc ggttttttgc tctgccttcg
     7381 cttgtgctgt tagtagcaag aggcgcatat tcgcttcttc cagcagcagt caaacggctg
     7441 ggatgaactc cgggattacg agtaatgact tttacgatag ctgttgc
//
LOCUS       DAOORU010000121         7484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_12166_length_7484_cov_5.773859, whole genome shotgun sequence.
ACCESSION   DAOORU010000121 DAOORU010000000
VERSION     DAOORU010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7484
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12166_length_7484_cov_5.773859"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..973)
                     /gene="pyrF"
                     /locus_tag="PLF20_07855"
     CDS             complement(143..973)
                     /gene="pyrF"
                     /locus_tag="PLF20_07855"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008563999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOX80950.1"
                     /translation="MNRQQLVSLIRRKKSMLCIGLDSDITKIPSHLLSYDDPVFEFNK
                     AIIDATLPFAIAYKPNFAFYESRGLVGWMSLKRTMDYLESFREEIFTIADAKRGDIGN
                     TSQQYAKAFFDKQSSGFDFDAATINPYMGTDSVSPFLEFPDKWVVLLTLTSNKGAVDF
                     QHLQKEGSKERLFEIVIKKSLEWGSTPSNMMYVVGATQASLLLKIRKILPEHFFLVPG
                     IGAQGGNLEEVLKYGSIREGGLLINASRSVIYASDGTDFSIRAAEVACALQCQMAAFI
                     "
     gene            complement(981..2054)
                     /gene="prfA"
                     /locus_tag="PLF20_07860"
     CDS             complement(981..2054)
                     /gene="prfA"
                     /locus_tag="PLF20_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOX80951.1"
                     /translation="MIDKLEAIYKRWLEIGETMNQPDVISDMKRYVKLSKDYKELMPI
                     VEAYKEYERICSNFESSKEMLNTEKDEELREMAKDEVEVLQEYKEKMEEKIRLMLLPK
                     DPQDSKNAIIEIRAGTGGDEASLFAGDLFRMYTRYCEIKGWKVEIVDFTEGSLGGYKE
                     IIFNVIGEEVYGTLKYESGVHRVQRVPKTESQGRIHTSAASVVVLPEADEVDVEIKPS
                     EIRKDIFCSSGPGGQSVNTTYSAVRLTHIPTGIVVSIQDEKSQLHNLEKAMNILRTRI
                     YEREYQKYLDNVASKRKTMVSTGDRSAKIRTYNYPQGRVTDHRINYSIFNLPAFMNGE
                     IQEMIDQLMLAENAERLKTEISE"
     gene            complement(2153..2872)
                     /locus_tag="PLF20_07865"
     CDS             complement(2153..2872)
                     /locus_tag="PLF20_07865"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOX80952.1"
                     /translation="MTKLSVNINKIALLRNARGGNLPDVVKAAMDCERFGAHGITVHP
                     RPDERHIRYSDVRAIKLVLTTEFNIEGYPIPKFLDLVLEVKPAQVTLVPDPPDALTSN
                     AGWDTIKNESFLREVVTELKNHGIRTSIFIETDARMIENAAKIGTNRIELYTERYAAS
                     YLMDAPKAIQPYVEAAQVALKCELGINAGHDLNLQNLKFFTQNIPGLLEVSIGHALIC
                     DALYYGLENTIQMYLRCLQPS"
     gene            complement(3025..4608)
                     /locus_tag="PLF20_07870"
     CDS             complement(3025..4608)
                     /locus_tag="PLF20_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HOX80953.1"
                     /translation="METKRAFLCSLILFFFLYSAEMRGQDSLSSNLIIQYEFNGDGTN
                     IVNTKYYLKIGDETKNYDFKWMPGRDGSERSALLFTGNSNGLKTSLNISPQILPELTY
                     IAWVYDRPDGFLFGSTLPVNSEKKFSSRSLQFSKGTVIAGFNKLDTLKKPYFASLQTS
                     KLKKDKWNFIALTVSSVDSVLRLYVNNEYFESKGVCYTPNNSFLIIGNTVNSPYTRPY
                     RGRVDEIRVYNKALTAAEISLISGFSISNAQERLERNATLKKALIAGLIAFLFLSVVY
                     MIVVLITERRYRPIKVSSFRSDSNKAPIDRKTEEANKLASDCVENAFETWETVSEEGG
                     QVLKSPTKHKHFKTTYKALAEAKSYNPDDIDVINRMNELGNLLNKLSKRKFYGNWFLA
                     ILTLIFPIVYFLIERNHLDTKGMIGVGVMLLPALAYILSNFAPNYVVANRTNKVGGIF
                     GKVIAFIMGAAASAVAVDYYTETTWSDGSKTTEYDTGSNAISLVIWVLLIIAAILLSM
                     ILAIIAAIIAFFRNYIFYI"
     gene            complement(5488..5832)
                     /locus_tag="PLF20_07875"
     CDS             complement(5488..5832)
                     /locus_tag="PLF20_07875"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80954.1"
                     /translation="MKAVKRLISTKRLPYLLKIYGRELTPEVILSCIYAVFYSNIYRE
                     KYAELLKIDFSRVPFPKDYKVFSKMAALVNELKDLHLMQSGRLDKLVSKYGGESDRID
                     MIVYRDSERRFI"
     gene            complement(6060..6506)
                     /gene="tsaE"
                     /locus_tag="PLF20_07880"
     CDS             complement(6060..6506)
                     /gene="tsaE"
                     /locus_tag="PLF20_07880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOX80955.1"
                     /translation="MMIVFRTSHLGELDHTASLMLSAFPNHRIFAVEGPIGSGKTTLI
                     KAFCQTLQIYDKVSSPTFSIINVYESPQGLVYHFDLYRLNDMAEFIATGAMEHLESGA
                     YCFIEWPNKALEILPHETIIIKINVEDDGARIYEMEPLPERTHKLR"
     gene            6733..>7484
                     /gene="rocD"
                     /locus_tag="PLF20_07885"
     CDS             6733..>7484
                     /gene="rocD"
                     /locus_tag="PLF20_07885"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOX80956.1"
                     /translation="MSNQLTSQQLIQLEENYGAHNYHPLPVVLAKGQGPYVWDIEGKK
                     YYDFLSAYSAVNQGHCHQKIVNALIEQAQKLTLTSRAFFNDSLGVYEKFITEYFGYDK
                     VLPMNTGVEADETALKLCRKWAYQKKGIPDGEAKIIVCEGNFHGRTITVISMSTDPDA
                     YREYGPYTPGFIKIPYNDLEALAKELEDPTVAGFLVEPIQGEAGVYVPDEGYLAKAYA
                     MCKEKNVLFMADEVQTGLGRTGKLLACDYENVR"
BASE COUNT         2295 a         1475 c         1374 g         2340 t
ORIGIN      
        1 ttaaaaaata attcatgata ttcgacatgg gtattctttt agtctcctcg tctccaaatc
       61 tcttattctc ctcgtccttt tgtctgccga tcaattagtc tccaaagaag cgtaatacaa
      121 accactcttt ttcaaagatt catcaaatga aagccgccat ttgacactga agtgcgcaag
      181 ctacttcggc agcacgaatc gaaaaatctg ttccatcgct agcataaata acactgcgag
      241 aggcatttat caaaaggccg ccctcacgga tcgatccata tttcaaaact tcttccaaat
      301 ttcctccttg agcaccaatt ccaggaacta aaaaaaagtg ttccggtaaa atttttctaa
      361 ttttcaaaag taaagaagct tgagttgcac caacgacgta catcatattc gaaggcgttg
      421 aaccccattc caatgatttt tttataacaa tttcgaagag ccgctctttg gaaccttctt
      481 tttgaagatg ttgaaaatca actgctccct tattgctggt tagcgttaat aaaacaaccc
      541 atttgtcagg aaattccaaa aaaggcgaaa ccgaatcggt acccatgtaa ggattaatcg
      601 tagctgcatc gaaatcgaag ccggaacttt gtttgtcgaa aaatgctttg gcatactgct
      661 gacttgtgtt tccaatatcg ccccgtttgg catccgctat tgtaaaaatt tcttctctaa
      721 aagattccag ataatccatg gttcttttca gactcatcca gccaaccagt ccgcggcttt
      781 cgtaaaaggc aaaattgggc ttgtaggcta tggcaaaagg cagtgtcgca tcgatgatgg
      841 ccttattgaa ttcaaaaaca ggatcgtcat acgaaagaag atgagatggg atttttgtta
      901 tgtcgctgtc gaggccaatg cacagcatag attttttacg tcgaataaga ctaacaagtt
      961 gttgcctgtt catgataaat ttattctgaa atttcagttt tcaatctttc agcattttcc
     1021 gccagcatca attgatcgat catttcttgt atttcaccat tcataaaagc tggtaggtta
     1081 aagatggaat aattgattcg gtggtcagtt acacgtcctt gaggatagtt gtaggtacga
     1141 atttttgctg agcggtcgcc agttgaaacc atagtttttc gtttcgaagc cacattgtcg
     1201 agatattttt gatattctct ttcgtaaata cgagtacgta atatattcat ggctttctcc
     1261 agattgtgta attgcgattt ttcgtcttga atcgaaacca cgataccggt aggaatatgg
     1321 gttaaacgaa cagccgaata ggtggtattc acactttgac cgcctggccc cgaggaacag
     1381 aaaatatcct tacgtatttc agaaggctta atctcgacat ccacttcatc agcttcggga
     1441 agaaccacca cgctggctgc tgaagtatgt atccttccct gagactccgt tttaggcaca
     1501 cgctgcactc tatgaactcc cgattcatat ttcagtgttc cgtaaacctc ctctcctatt
     1561 acgttgaaaa ttatttcctt ataaccacca aggctacctt cagtaaaatc tactatttcc
     1621 actttccagc ccttgatctc gcaatagcgg gtgtacattc ggaacagatc gcctgcaaac
     1681 aaagaggcct catctcctcc tgtacccgcc ctgatttcga taatggcatt cttgctatcc
     1741 tgaggatctt tcggaagtag catcaaacga attttttctt ccatcttttc tttatattcc
     1801 tgtaatacct ctacctcatc cttggccatt tctcgcaatt cctcatcttt ttcggtgttg
     1861 agcatttctt tcgagctttc gaaatttgag caaatccttt cgtactcttt ataggcttca
     1921 acaatgggca tcaattcttt atagtctttg ctcagcttca catatcgctt catgtcggag
     1981 ataacatcgg gctggttcat tgtttctcca atctcgagcc aacgtttata aatagcttct
     2041 aatttatcga tcattccttg aaatatcttc gaaaaaaagt ctgcaaaatt actgaacttc
     2101 tgtcagtaaa tttgaccgga taataaaaaa ccaaaaaata tccttatgcc tgttacgatg
     2161 gttggaggca acgcaaatac atctgaatgg tattttcgag gccataatag agtgcatcgc
     2221 aaattagggc atgaccaatc gaaacctcaa ggaggcccgg aatgttttga gtaaaaaact
     2281 ttaaattctg aagattgaga tcatgaccag cattgattcc taattcacac ttcaaggcca
     2341 cctgtgccgc ttcaacataa ggctgaatag ctttaggggc atccataaga tacgaggcag
     2401 cataacgttc ggtgtatagc tcgatgcgat tggtgccaat cttggcagca ttttctatca
     2461 tgcgtgcatc agtttcgata aatatggagg tgcgaattcc atgatttttc aactcagtaa
     2521 ctacttccct gaggaaggat tcgtttttta tcgtatccca tccagcattg gaggtaagcg
     2581 catcaggtgg gtcaggaaca agtgttacct gagccggttt aacctcgagt accaaatcca
     2641 gaaattttgg aattgggtat ccttcgatat tgaactcggt ggtaaggacg agttttatgg
     2701 ctctgacatc actgtagcgg atgtgccttt catcgggccg ggggtgtact gtaattccat
     2761 gtgcaccaaa acgttcacaa tccatagccg cttttactac atccggaaga ttgcctccac
     2821 gagcatttct aagaagggca attttgttga tgtttacact taattttgtc atatcgatga
     2881 actatccaat tgattttaca aaaatactgt taatctctcc aggcaaaaca tgacggaagt
     2941 tttagaccct tagtgtggga aaaacgtttt gaggtaaaat tctcgagtat aaaagaattt
     3001 tggtcataac aaaaagctca ccctctaaat gtaaaaaata tagttgcgga aaaaagcaat
     3061 gatggcagct ataattgcca aaatcataga aagtaggatg gccgcaataa tgagtaaaac
     3121 ccaaataaca agagaaatgg catttgagcc tgtgtcgtat tcagtcgttt tacttccatc
     3181 actccatgtt gtttcggtat agtaatcgac ggcaactgcc gatgctgccg cacccataat
     3241 aaaagcaatg accttgccga aaatgcctcc cactttgttg gtgcgattgg ccacaacata
     3301 gttgggagca aaatttgaca aaatataagc aagtgccggt agtaacatta ctcccactcc
     3361 tatcatccct tttgtatcga gatgattacg ttcgattaaa aaataaacaa tggggaaaat
     3421 caacgttaaa atagccaaaa accagttgcc ataaaacttc ctcttactta gtttattaag
     3481 taggtttccc aattcgttca ttcggttgat gacgtcgata tcatcagggt tgtatgattt
     3541 tgcctcagcc aaagctttgt aagtggtttt aaaatgctta tgttttgtag gagattttaa
     3601 aacctgaccg ccttcttcac tcacggtctc ccacgtttcg aatgcatttt caacgcaatc
     3661 agatgccaat ttattcgcct cttcagtttt tcgatcaata ggtgccttgt tgctatcgct
     3721 ccgaaatgag gatactttaa tgggccgata acgtctttcg gttatcaaca ctactatcat
     3781 atatacaacc gacaagaaaa gaaaagcaat caagccagct attaatgcct ttttgagcgt
     3841 agcatttcgt tctaatcgtt cctgtgcgtt tgaaattgaa aagccactga tgagtgaaat
     3901 ttcagcggct gtaagagctt tattataaac tcgaatctca tccacacgac cccgataagg
     3961 tctggtgtaa ggagaattca cagtgtttcc aatgattaaa aagctattgt taggagtgta
     4021 acatactcct ttcgattcga aatattcatt attgacgtaa agacgcaata cagaatcgac
     4081 tgaactaacc gttagagcaa tgaaattcca tttgtccttt tttagtttcg aggtttgcaa
     4141 ggaagcaaaa tagggtttct tcaaagtatc caatttattg aatccggcaa tgacagttcc
     4201 tttcgaaaac tgaagcgaac gagaagaaaa ctttttttcc gaatttactg gaagtgtcga
     4261 tccgaaaaga aatccgtccg gacgatcata aacccaggct atataagtaa gctcgggtaa
     4321 tatttgtggc gaaatattca gggatgtttt caatccattc gaattgcctg taaataaaag
     4381 agccgaacgt tccgatccat ctcgtccggg catccatttg aaatcgtagt ttttcgtttc
     4441 atcaccaatc ttgagataat acttggtgtt aacgatattc gtaccgtctc cattgaattc
     4501 gtattgaatg atcaagttgg acgataaact atcctgcccc ctcatttcag cagaatacaa
     4561 aaagaaaaat aatatcagtg aacacaaaaa agctcgttta gtttccatat aaaaaattat
     4621 ttaaatttaa taaagatgtt tattttttga agaaattgta taaaaatgtc attcgttaat
     4681 ataaaaaatt catgttaaaa tttgatagac agatcaaaaa aaataaataa aataatgcta
     4741 tgacaaaaaa taaaattaaa agatttttaa agaactaaga aaaattttaa gaaaccaatt
     4801 tgtagattac aattaattta ataatttaca ataagttgaa aaaggataat ataattttat
     4861 aatatttgaa atatttattg taatgttctg atttgtctat tataggaaag aatagatctt
     4921 ttgaattttt atcttcaatc tatttcacct caaaaaacaa tttttcgaaa gaaaccacaa
     4981 acgtatgaat tcttttctgt ttacaaatat ttattatgaa atttctgaaa ttttttttag
     5041 tagttggcat aaaacattgt atagccgaaa attataatta ggtttcaggg gcacctctta
     5101 ccaaattatg atctatgaag cgtcaagaat ccccttttct gatatcatca ttttaacacg
     5161 gatgaaagca atgaaatacc aacccgctta acgattgcta tcaagagtaa tcttatcttt
     5221 ttggatttta aattcttctg tatgggatag tctaaaaact ttccctttaa aattcgatga
     5281 tcctcttttc aacttcggga taggtcttgc caatttcttg ctgtgttata atgttgaggc
     5341 tcaatgcggt aatgattttg caataattca cagcctcata tatatttctt cctttcctat
     5401 ctttcagaaa tttataaagc tcctgatatc ctccaatctc atagttccaa acttcaggct
     5461 gtacattatc aaaatatttt tcatcgttta tataaatcgc ctttctgaat cacgataaac
     5521 aatcatgtca atcctatcac tttcaccccc atattttgat acaagtttat ctaatcgtcc
     5581 actttgcatc aaatgcaaat ctttcaactc attcaccaaa gcagccattt tgctgaaaac
     5641 cttataatct tttgggaaag gcacacgcga gaaatcaatt ttcaaaagct cggcatattt
     5701 ctcacgataa atattgctgt aaaaaacagc gtaaatgcag gacagaatta cttcgggcgt
     5761 aagttcacga ccataaattt ttaaaagata cggtaacctt ttcgtcgaga ttaggcgttt
     5821 tactgctttc atattccatt ttaggttcaa acaaaatcat catcgacgag gaatgcgatt
     5881 tttggggagc ttgctctttg taaaggtaaa gtggaaataa agataattga tatccaacga
     5941 tgtaattgct gtagctcctg ctatcatctt atcgcttacc aaagtataat tcaatttttt
     6001 gagatttctt gaacgtggct taacccctta tattgataca atcataactc atataggttt
     6061 taccgaagtt tgtgggtcct ttctgggagg ggttccattt cataaattcg ggcgccatcg
     6121 tcttccacat ttatttttat gatgatggtc tcatggggaa gaatctccag agccttatta
     6181 ggccattcaa taaagcagta agctccactt tcgaggtgtt ccatggcacc agtagctata
     6241 aactctgcca tgtcgttcag gcggtaaaga tcgaagtgat atactaaccc ctgaggtgac
     6301 tcatatacat tgatgataga aaaggtgggg cttgatactt tatcgtaaat ctgtaatgtc
     6361 tggcaaaaag cctttattaa ggtagttttc ccgctaccga taggcccttc cacggcaaaa
     6421 atcctatggt tgggaaaagc cgagagcatc aggctggccg tatggtctaa ttcgcctaaa
     6481 tgtgaagttc taaaaacaat catcatctga ttttagtgat ttcaaaatga aataaaactg
     6541 cttagtatcg tttttgaatc atgaatttgc aaaaatattt tatttggcgc acatttagca
     6601 gattagcagc attttatcca atagacagag tagatatctg gtaactgcat ttttttatat
     6661 tccactaaat agctaatttt gctaggtttg aataggattg aggaatacta acgttttata
     6721 aaattttata ttatgtcaaa tcaactcaca tctcaacaac tcattcaatt agaagaaaat
     6781 tatggagcac acaattacca tccattgccg gtagtgctgg caaagggtca gggaccgtat
     6841 gtttgggaca tcgaagggaa gaagtattat gattttcttt cggcttactc ggctgtaaat
     6901 caaggacatt gtcatcaaaa gattgtgaat gcactaattg agcaagcaca aaaactcacc
     6961 ctcacttcac gtgcattctt caatgattcc ttaggggtgt atgaaaaatt cataaccgag
     7021 tattttgggt atgataaagt tttacccatg aatacaggtg ttgaagccga cgaaactgcc
     7081 ctgaaattat gccgcaaatg ggcctatcag aaaaaaggca tacccgatgg cgaagcaaag
     7141 attatcgtat gcgagggtaa ttttcatgga cgcaccatca cggtgatttc gatgtcgacc
     7201 gaccctgatg cttaccgtga atacggtcct tatactcccg gtttcattaa aattccttat
     7261 aacgatttag aggctttggc aaaggaattg gaagatccga cagtggcagg gtttctggtt
     7321 gaacccattc agggcgaagc aggcgtatat gttcctgatg aagggtattt agcaaaagct
     7381 tatgctatgt gtaaagaaaa gaatgtactg tttatggccg atgaggtaca gacaggatta
     7441 ggacgtacgg ggaagctact tgcttgcgac tatgaaaatg tacg
//
LOCUS       DAOORU010000122         7408 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_12324_length_7408_cov_6.243166, whole genome shotgun sequence.
ACCESSION   DAOORU010000122 DAOORU010000000
VERSION     DAOORU010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12324_length_7408_cov_6.243166"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            476..1903
                     /locus_tag="PLF20_07890"
     CDS             476..1903
                     /locus_tag="PLF20_07890"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOX80957.1"
                     /translation="MENLSITHQLFRMSKLPFIQGYLLKKVDKYLYDIIVEENKRNLE
                     SVKMRKYHFISAMMHSAMKNVRKGRISTYAIQRLNEVLVENAFFKAPEQMKNAKEAFK
                     DKFGYDAPSFITLSPTQACNLKCSGCYASSDPHAMASLPYSIVDRLTGEVHDSFGSRF
                     ITISGGEPFLYKSEGHTLIDLFDKYKDMFFLVYTNGTLITKELAHELARVGNATPAIS
                     VEGFEKETDDRRGKHVHKRILETFHNLRKSGVPFGISVTASNNNIQTLLQDKFYDYYF
                     DGLGATYMWLFHFFPIGRGKEQFDLMLKPADRLKLYEMWEKQIAQKKHCIADFWNSGV
                     LTCGCIAYGGNRGFLYIDWNGNIMPCVFVPYYQHNIIDLYNSGKDLTYALQSDFMKNG
                     RKWQQEYGLNNQKSPNNWLMPCSIRDHYDNFRKNILTPEAKGENQEAQEILDDSLYYE
                     KMTLFDEELKKLTDPVWKSKYLNEG"
     gene            2442..2999
                     /locus_tag="PLF20_07895"
     CDS             2442..2999
                     /locus_tag="PLF20_07895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOX80958.1"
                     /translation="MIKVGIIIGSTRPGRNAEAVGKWVYEIAIKRNDATFELVDLKDY
                     NLPLLDEAIPASMHQYEHEYTRRWAEKIASLDAYIFVTPEYNHAPTAALKNALDYIYT
                     EWNNKAAGFVSYGSMGGARAVEQLRQIMSELQIADVRAQVSLSLFTDFENFSVFKPAP
                     MHDTYLNTMIDQLVAWGEALKTVRK"
     gene            3003..3257
                     /locus_tag="PLF20_07900"
     CDS             3003..3257
                     /locus_tag="PLF20_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80959.1"
                     /translation="MNASEKLPVFYTPHGGGPWHVMEDYMGDPEGYGMLKNYLEKLGK
                     EYREKIESLLVISAHWEEPLPAVHFGSNPSMFYDYYGFNK"
     gene            complement(3520..>7408)
                     /locus_tag="PLF20_07905"
     CDS             complement(3520..>7408)
                     /locus_tag="PLF20_07905"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HOX80960.1"
                     /translation="EKVSLRGKAIDYSGFPIGQAKVSYRVTRSAFSPYPRSKWWPPLI
                     PSEETEICNMTTTTDEKGFFNVDFTAYPDPSSANDPKIAYRFFIEATIVDLNNETHEA
                     GTSLSIGNRALISYVEVPSQVDKNQPVNFKIISTNLSGKPVNSRGTIYINRMISPKKI
                     YYPRPFSNPDLPVIPMNIFQTAFPQYPFQNEDEFDQWEKGEVVLSQGYDTKKDSILRI
                     NEFGNWPSGVYRVSIYAIDTFGQPIENQRYFVLYDTHEKKPPYPTPLWFVAEQNSYEP
                     GQTARFIIGSSEKNAKIFLQVVLPDSVIMEDWIKLSNQQKLVEIPVKERYRGNFKINL
                     LFFKNNHVFYESQTIEVPYSNKKIEFTLSTFRSTLEPGAEETWQISLKEPSNKPVLAE
                     VLANMYDASLDVFQNSAWNIPSYSPNSIFSWWRIFNQNSVGGYYLNSNIGPYLPVKEI
                     KFDRIDWFGYPIYARYKMYRGAVSAGKTQQPNYELVVTQNAEELEADEGVMVYNKIYD
                     DRTPKKVSIRKDFSETAFFMPHLTTDSNGRLQLKFKMPESLTRWKFRLFAHTQDMKLG
                     FIEKEIITAKQLMVVSNAPRFLREGDTLIFPTKLTNMSDNDLEGNIRLEFFDLLSDKP
                     LHLIAKGDSSVKIFSTRAGLSDQYEWKIIVPSGTSVLRYRLTAQCESHSDALEDLIPV
                     LSDQIWLGDAHPLFAAASQTTDFEISMNPEILHDSRTHITFEFTTNPAWIVLQALPSI
                     SLPERQSVDAWFASFYANAIGKNIIEKNPSIRPVIEAWKRYQPSALQSNLQKNEDLKN
                     IVLNETPWVGQAETESASKNQLADFLDENKMNNDLQIAFQKISQMQSSNGGFSWFEGM
                     PDSRYITLEIVKGLSRLLHQQMIDIEDYDGLDKLLSSAVDYLDQRVLEDYKNELEHLV
                     RDKNYQGQLSYTMAKYLYARSLLVNAFPIDQKYKESWDYYMNLARRDWIKQSLGTQAL
                     LAISFFENKEDRQARTILKSLRDRALFDNEKGLYWARKHNRWMAEGELETQAAMIEAF
                     MYDESYYSDVENMKKWLIYKKRTQNWGTSMATTEAVYALLLKGKPLLNENKPVKITVN
                     GESLLANESGDLAIEAGSGYFRKTWTNSQNIPNIMKIHIENLNDIPIWGGMYVQYFKP
                     IDEVEASSGGLKIEKQLFMERLGESSGPVLMPISPSRQLEAGNKVVVRLIVENDNDLS
                     YVHLKDYRATGFEPMDVISGYRWQNGTAYYLTNSDVATQFFFEYLPRGKRVYEYSLRV
                     FQKGSYSSGFASLQCLYAPEYSAHSGSIHVKTK"
BASE COUNT         2268 a         1486 c         1375 g         2279 t
ORIGIN      
        1 ttttatcact aaatttgtct aaccaaaaag ttgcatttgt gacttgaatt cagcttatat
       61 taatatacat ataaaataag atttatatcc ccatgcggaa tgcaaatcaa aacattgatt
      121 aaccctaatt tatcgatttt actttaatac tcataaccct ttctttatct ttcctttagt
      181 taaatttacg aaaggtgcct actactatcg aagaaaatac aattgatgct actccgcgat
      241 ttttatttta tataatcctt taaaacatat atacggtttc atttttattc tctaaataat
      301 ttcttcaaca atttataaca gaattatctt gaatttgatt acgattttgt atctcatttt
      361 cttcattgtt gcttaaattt gtcatggttt tcaaaagagg caaaatggaa tgaagaattt
      421 tgaccgggtt ttgtttacgg aataattgaa ttagaacgtt ttaaaaacat atatcatgga
      481 aaatttgtcg attacccatc agctgttcag aatgtcgaaa ttacctttta ttcagggata
      541 cttgctcaaa aaagtcgata agtatcttta tgatattatc gtcgaagaaa ataagcgaaa
      601 tctcgaatcg gtaaaaatga ggaagtatca tttcatttcg gccatgatgc actcggcgat
      661 gaagaatgtg aggaaagggc gaatatccac ctatgccatt caaaggctca atgaggtgct
      721 agttgagaat gcttttttca aagcgcccga gcaaatgaaa aatgctaaag aggcatttaa
      781 agataaattt ggctacgatg cccccagttt tataacgctt tcacctactc aggcctgtaa
      841 tttaaaatgc agcggatgtt atgccagctc cgatcctcat gctatggcta gtctccctta
      901 ttctatcgtc gaccgtttaa ccggcgaagt tcatgattcc tttggaagcc gttttattac
      961 catcagtgga ggtgaacctt tcctttataa aagcgaagga catactttaa tcgatttatt
     1021 cgataaatat aaggatatgt tcttccttgt ttatacgaat ggcacactga ttaccaaaga
     1081 attagcacat gaacttgcac gagtaggcaa tgccactccc gccatttcgg tggaaggatt
     1141 cgaaaaagaa accgatgaca gacgtggcaa acacgtccat aaaagaatcc ttgagacatt
     1201 ccataacctc cgcaaatctg gagtaccctt cggaatttca gtcactgctt ccaataataa
     1261 tatccaaacc ttacttcagg acaaatttta tgattattat ttcgatggat tgggagcaac
     1321 ctatatgtgg ctgtttcatt tctttccgat tggcagaggc aaagaacaat tcgacctcat
     1381 gctaaaaccc gccgatcgcc tcaaacttta cgagatgtgg gaaaaacaaa ttgcccagaa
     1441 aaaacattgc atagccgatt tctggaatag cggagtgctt acctgcggat gtattgccta
     1501 tggcggaaat cgcggcttcc tctatatcga ctggaatgga aacatcatgc cctgtgtgtt
     1561 cgtcccttac tatcaacaca atatcatcga tttatacaac tcaggcaagg atctcactta
     1621 tgcccttcaa tccgatttca tgaaaaatgg ccgaaaatgg caacaggaat atggattaaa
     1681 caatcaaaaa tcgcctaaca actggctcat gccttgctct atacgcgacc actatgataa
     1741 ctttcgaaaa aatattctta ccccggaggc aaaaggtgaa aaccaggaag ctcaggaaat
     1801 cctcgacgac tccctttatt atgaaaaaat gactttgttc gacgaagaac tgaaaaaact
     1861 gaccgatcca gtctggaaaa gtaaatatct gaatgaagga tgaaacctac tacattatcc
     1921 tctctggtaa aaattccaag acagtaggat aaaatcgaca atatggccta aacttcctaa
     1981 atactttcat tttcctttgc aaacctatct atcgaaaatt ttccttccac tcaaattgtt
     2041 gtttgaacgg cgaggaagat tctatggcaa tgattccttt caaccatctt gcgttaaata
     2101 agtatccatc agtttacaat ttggtttcat tcgtaaacaa tttttgaatg aaaggataga
     2161 acattttgat tgaattggtt gttattgaat aatagttcca taaccggctc tgttttcaaa
     2221 agctaccttc tattttataa taaccaataa atcaataata taatattaaa tatcaaaaaa
     2281 atcaacagaa atttaaaatt agatgtcata acataatttt tatatattta catatattag
     2341 taaaactttt aaaatgactt ataaaagaaa aacgaatgaa acataaacta aaggatatca
     2401 acaatcaata aactattaaa acatcttaaa ttattaaaaa catgataaaa gttggaatca
     2461 tcattggaag tacgcgaccc gggcgcaacg ctgaagctgt tggcaaatgg gtctacgaaa
     2521 ttgcaatcaa aagaaacgat gccacatttg agttggtaga tttgaaagat tataatcttc
     2581 ccttgctcga tgaagccatt ccggcctcca tgcaccaata cgaacatgaa tacacccgca
     2641 gatgggctga aaagattgct tcattagatg catatatctt tgtcacgcct gaatacaatc
     2701 atgctcccac agcggctttg aaaaatgccc tcgattatat ttataccgaa tggaataata
     2761 aagctgccgg atttgtcagc tatggttcta tgggtggggc acgtgcggta gaacaattgc
     2821 gccagattat gagtgaattg caaatagccg atgtacgcgc tcaagtttct ctttcacttt
     2881 tcactgattt tgaaaatttc agcgtattca agccagcgcc aatgcacgat acctatctga
     2941 atacgatgat cgaccaattg gtggcctggg gcgaagccct gaagacggtc aggaaataaa
     3001 ccatgaatgc ttctgaaaaa ttacccgtat tctatacccc tcatggagga ggaccatggc
     3061 acgtaatgga agattacatg ggcgatcctg agggatatgg aatgctaaaa aactatctcg
     3121 aaaaacttgg aaaagaatat cgtgaaaaaa tagaatctct tttagtcatt tctgcccatt
     3181 gggaagaacc cttgcctgcg gtacattttg gctctaatcc ttccatgttt tacgattatt
     3241 atggattcaa caaatagaaa ataaattcat agcattaaat aacccatcat tttcgcaaaa
     3301 ggatgaaaat tgatgatgaa atcaacggac acctcagaaa tgagtatcgt agaggtttga
     3361 tcaatcttat acgaagaagc gaacgagctc aaccgcttat tggataaaat caaagaaaag
     3421 gattctaaat gtcactcaaa aagaaaaatc cattcaccgc tatcgggctt catcaaattt
     3481 cggtatcaat aaaatcgagc cttgtcttgc tcgaactttt tattttgttt tcacatgaat
     3541 acttcctgaa tgtgcagaat attctggagc gtaaagacat tgcaaacttg caaaaccgct
     3601 gctgtaggat cctttctgaa atactctcag tgaatattca tagactcttt tcccgcgtgg
     3661 gaggtattcg aaaaagaatt gtgtagctac atcactattg gtaagataat aagcggtacc
     3721 gttctgccat cgataacccg aaattacatc catgggttcg aaacccgtag ccctgtaatc
     3781 cttcaaatga acataactca agtcgttgtc gttttcgacg atgaggcgaa cgactacctt
     3841 attacccgct tcgagctgcc gggaaggaga aatcggcatc aaaacgggtc ctgatgattc
     3901 tcctaacctt tccataaaaa gttgcttttc gatttttaaa ccaccactgg aggcttctac
     3961 ttcatctatt ggcttgaaat attgaacgta cattcctccc caaataggta tatcattgag
     4021 attttcgata tgaatcttca ttatgttggg aatattttga gaatttgtcc atgtcttcct
     4081 gaaataccca cttccagctt caatggctaa atctcccgat tcgtttgcca aaagactttc
     4141 accgttaaca gtaattttta ctggcttatt ttcattcaaa agaggtttcc ctttcaagag
     4201 cagggcataa accgcctcag tcgtagccat ggaggttccc cagttttgag tccgtttttt
     4261 ataaatcaac cattttttca tgttctctac atccgaataa tagctttcat catacataaa
     4321 ggcttctatc atggcagctt gggtttccaa ttcaccttcg gccatccatc tattgtgttt
     4381 tcgtgcccaa taaagtcctt tctcattgtc gaaaagagcc cggtcgcgga gcgatttcaa
     4441 aattgttcgc gcttgcctat cttccttatt ttcgaagaaa gatatggcca gtaaggcttg
     4501 ggtgcccaag gattgtttta tccagtcgcg cctggccaaa ttcatataat aatcccatga
     4561 ttccttatat ttttgatcaa taggaaaagc atttaccaat aaacttctgg catataaata
     4621 tttagccata gtataactca actggccttg ataattttta tctctaacca aatgttcgag
     4681 ttcattctta taatcttcca acactcgttg gtcgagataa tccactgccg acgaaagaag
     4741 cttgtccaat ccatcataat cttcaatatc aatcatctgt tgatgtagga gcctgctcaa
     4801 acctttcaca atttccaaag taatatatct tgaatcgggc ataccctcga accagctaaa
     4861 tccgccattg gatgattgca tttgactaat cttttgaaaa gctatctgca agtcattgtt
     4921 cattttattt tcatcgagaa aatcggctaa ttgattttta gaggcagatt cagtttcggc
     4981 ttggcctacc catggtgttt cattgagcac aatatttttc aaatcctcat ttttctgaag
     5041 attcgactgc aaagcagacg gctgatatct tttccatgct tcaataacag gccggatgga
     5101 aggatttttc tcgataatgt ttttcccgat ggcattggca taaaatgagg caaaccatgc
     5161 atcaaccgat tggcgttcgg gcaaagaaat ggagggcaag gcttgaagta caatccatgc
     5221 aggatttgta gtaaactcga aggttatatg ggttcgacta tcatgcaaaa tttcaggatt
     5281 catagagatt tcgaaatcgg tagtttgcga agcggcagca aataaaggat gcgcatcgcc
     5341 aagccagatt tggtcagaca gaacaggaat caggtcttcg agagcatcac tatggctttc
     5401 acattgcgca gtcaagcgat agcgcaaaac acttgtgcct gagggaacta ttatcttcca
     5461 ttcgtattga tcacttagtc ctgctctggt cgaaaaaatc ttgacagagc tgtcaccttt
     5521 cgctattaaa tgaagaggtt tgtccgaaag taaatcgaag aattcgagac gaatgttgcc
     5581 ttccaagtca ttatcggaca tgttggtgag ttttgtcggg aaaatcagtg tatcgccttc
     5641 acgcaagaat cgaggagcat tcgatacgac cataagctgt ttggcggtaa taatttcttt
     5701 ctcgatgaat ccaagtttca tgtcctgcgt atgagcaaac agccggaatt tccatctcgt
     5761 gagcgattct ggcatcttaa atttcaactg taagcgtcca ttgctatcag tggtcaaatg
     5821 cggcatgaaa aaggcggttt cgctgaaatc cttacgaatt gaaacttttt ttggtgttct
     5881 atcatcatat attttgttat agaccatcac tccttcatca gcttctaact cctcagcatt
     5941 ttgtgttacc acaagctcgt agtttggttg ttgtgttttt ccagccgaaa ctgcacctct
     6001 atacattttg tatcgtgcat aaattggata accaaaccaa tctatcctgt cgaattttat
     6061 ttctttaaca ggtaagtatg gcccaatgtt gctattcaga taatatcccc ctacgctatt
     6121 ttgattaaaa attctccacc agctaaaaat agaatttggg gaataagagg gaatattcca
     6181 ggctgaattt tggaatacgt cgagggaggc atcatacata ttggccaaca cttccgctaa
     6241 tactggcttg tttgaaggtt ctttcaggct aatttgccaa gtctcttcgg caccgggttc
     6301 caacgtcgaa cgaaaggtgc tgagcgtaaa ttcgattttc ttgttcgaat aaggaacttc
     6361 aatggtttgg ctctcataaa aaacatgatt gttcttgaaa aataagagat tgatcttaaa
     6421 attccctcta tatctttctt ttacaggtat ttcaaccaac ttttgctgat tgcttagttt
     6481 gatccaatct tccatgatca cactgtcagg gagtacaacc tgaagaaaaa ttttcgcatt
     6541 tttttcggat gaacctatta tgaatcgagc cgtttgtcct ggttcgtaag agttttgttc
     6601 agcaacaaac cacaaaggtg taggataagg tggctttttt tcgtgagtgt catataaaac
     6661 aaagtacctc tgattttcaa ttggctggcc aaatgtatca atggcataaa tactgaccct
     6721 gtaaacacct gaaggccaat tcccgaattc atttattctt agaatgctat ctttctttgt
     6781 atcataacct tgtgacagca caacttctcc tttttcccat tgatcgaatt catcttcatt
     6841 ctgaaaagga tattgaggga aagccgtttg gaagatattc atgggaataa caggtaggtc
     6901 ggggttacta aaaggtcggg gataatagat tttcttggga gaaatcatcc gattgatata
     6961 aatagttccg cgcgaattca ccggtttccc ggaaagattt gttgatataa ttttaaagtt
     7021 gaccggctga tttttatcaa cctgagaggg aacctccaca taggatatca atgctcgatt
     7081 ccctatcgaa agcgaggttc cagcttcgtg tgtctcgttg ttaaggtcta ctatagtagc
     7141 ttcaataaag aagcgataag ctattttagg atcatttgcc gatgaaggat cgggataagc
     7201 agtaaaatca acattgaaga atcccttttc atctgttgtt gtggtcatat tgcaaatctc
     7261 cgtttcttcg gatggaatca gcggaggcca ccatttcgag cgtggataag gagagaaggc
     7321 agaccttgtt actctgtagc ttaccttagc ttgcccaatt gggaagccac tgtagtcaat
     7381 ggctttacct ctcaggctca ctttctct
//
LOCUS       DAOORU010000123         7291 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_12567_length_7291_cov_5.041874, whole genome shotgun sequence.
ACCESSION   DAOORU010000123 DAOORU010000000
VERSION     DAOORU010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7291
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12567_length_7291_cov_5.041874"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PLF20_07910"
     CDS             <1..933
                     /locus_tag="PLF20_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOX80961.1"
                     /translation="IYIFPSLYGEGYDALMLMLNGHGTEVVNQSLFGNLQMSSFMFPL
                     ILLVIMLTKVIAMALTNAAGGIGGVFAPSLFMGGMSGLFFTRTLDLLTGIRMPENNFV
                     LVGMAGVMAGVMHAPLTAIFLIAEITGGYQLLTPLMLTAAISFITIQYFEPHSIYTKK
                     LAERGELITHHKDKAVLSMMKVTDLLETNFKTVFPDATLGEFVKVVASSERNVFPVVD
                     KENNFLGVVFINDIRHILFQQELYHKIFVKDLMFMPDVKVSPDDTMEDVAKLFQQTAH
                     YNIPVIKDGKYLGFVSRANVFSNYRKLLKEFSED"
     gene            1047..3026
                     /locus_tag="PLF20_07915"
     CDS             1047..3026
                     /locus_tag="PLF20_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HOX80962.1"
                     /translation="MFKNHPKGLLVLAITNMGERFGYYTMLAILTLYMQAKFGLTSAT
                     TSMIYGTFLALVYFLPILGGVIADKALGYGKTILLGILVMFIGYLLLAIPLESNAVGL
                     AMMIFSLVLIALGTGFFKGNLQALVGKLYDDPRYGKNRDIAFSIFYMFINIGAFFAPS
                     AANYINNTVLRSADFTYDASIPKNYVILNDEKNAIDKNTFILKYISPEEQKLSGKEFE
                     SVLNKAKKEHKAVFTDDIEFALFRLKAAGLYQYTQSGKITDSSMMISSHEYELLQSRD
                     PKDAQDKADVITKIQAIHVTDPNLGGGPNPDDFARNFGKHYVSHSLSTSYNLAFGVAC
                     ISLIISVLIFLLFKKTWKAVDKTVKQEIAEAKITGKEVKMMPKEEVRERMIALFLVFF
                     VVIFFWMSFHQNGLTLTFFARDYTMSNIPAAWTMLFNLFPLLSLIVFLYGIYLSTIGL
                     LGSKPNRKIGILLVLFGIAGMAGYYFTSIYGIDKMTKITPQIFQQFNPMFIIILTPLT
                     VALFGYLASKGKEPSAPRKIGIGMFLAAVGYAIMMICSIGLPAPAEISGISDKLVSPS
                     WLIGTYFVLTIAELFLSPMGLSFVSKVAPPQYSGIMQGGWLAATAIGNLLVGVVGTFW
                     EKLPLVGFWSILIVCCLLSGIFIFSIMKKLETATE"
     gene            3101..4054
                     /locus_tag="PLF20_07920"
     CDS             3101..4054
                     /locus_tag="PLF20_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108921.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HOX80963.1"
                     /translation="MKYPWGDDNPYYSYSTYSKRIFGGRIQKLSIKAGFTCPNRDGTK
                     GIGGCYYCNNDAFNPSYCRSDKSITQQIEEGIAFHAWRYRRATKYLAYFQAFSNTYAP
                     LSHLQKLYGEALSHPAVVGLVISTRPDCLPEETLIYLSQLSKEYFIQVEIGIESCYDA
                     TLSRINRGHSFDDTIKALDNLSMRGIMTGAHLIFGLPGETIEMMLNEAQIVSQLPLTS
                     IKLHQLQIIEGSVWATEYRLRSESFRLFERNEYVDFIIKFIERLRPEIVVERLSAEAP
                     PRFVIAPDWGLERADAFQQDIREKMLSLQTYQGKLFKFNEK"
     gene            4152..6590
                     /locus_tag="PLF20_07925"
     CDS             4152..6590
                     /locus_tag="PLF20_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX80964.1"
                     /translation="MKKLYSLIVAMFLIVGAFAQTPPYTDNFESYTVGGYLAAQNPDW
                     WTTWSNTPGTGEDAIISNAHANSGAQSVLVDETGGSTDLILKLGNKTVGLYYIGWYVF
                     VDNGKAGYYNIQHFQSPGTEWAFEVYFNTNGSGTLKAGGNSITFSYPKATWFKVYHEI
                     DLDNDQIKLYVNGTQVHQWPFSYQASSTTGTKQLGAVDFFAGAQTGETPQYWFDDISF
                     ELAPAPLYSQNFDSWAVGSYVAVNDPEWFTTWSNSPGTGEDALIDNAFSHSSSNSARV
                     DETDGATDLILKLSNKTSGHYNLSWYMYIENGKAGYYNIQHFQSPGTEWAFEMYFKAN
                     GTGKLLEGNNTITFTYPQATWFKVEHDIDIDNDWIKLYVNNVLIEEWPFSYQSGSTTG
                     TNQLGAVDFFAGAEQGSGETPKYFFDDVVFMAYGAIPEANIEVDPTSLAAYLEPNSTI
                     TEQITITNSGSLDLSFDALVILNSGKMAGKATATPQPAVLTNAFAGPVLNGGAPSTDA
                     TAVLHYDGDNYSAIGWSSAPVTVTVAAMFPYTMTAPYAGMKINSVDVYINDLNSSGSN
                     EMTVKIYGMGLTYEPGPLIYSQPFTPMGGGWEHIVLTTPVLVTGEDIWVGYQFTQNET
                     GIYIPGTDAGPNHPYGDFLSTGVGWSHLSNNPSLLYNWNIRANLNGDPFNKWLTITPT
                     SGTIAPAGSQILDVTFNSNNQPVGIYEGIVRIMSNDPDSPSVDVLCTLSVSVGINDMN
                     KVAVLVYPNPAQDFINVAANQKITEIRIMNFAGQTLYKGNENHINISNLPKGIYYLQT
                     ITHEGISNIKFIKN"
     gene            6789..>7291
                     /locus_tag="PLF20_07930"
     CDS             6789..>7291
                     /locus_tag="PLF20_07930"
                     /inference="COORDINATES: protein motif:HMM:NF037004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80965.1"
                     /translation="MKYCKPVLLFVLFILILPGCGAQVDEQKRNLILMDLLKRSVEEG
                     HFSPVAMDDQYSKKAYNLFIEQLDPLKRFFTEDDLAEMKKYELRLDDEFKNYDTEFFE
                     LTYNILSKRLKQIEDFYSEILKRPFDFGKEEYLEMDPDKRRYPKNDEELKTAWEQMLK
                     YEVLNWLT"
BASE COUNT         2083 a         1500 c         1489 g         2219 t
ORIGIN      
        1 atctatatat ttccttcact ttatggggaa ggatatgatg ctttgatgct gatgctcaat
       61 ggccatggca ccgaagtggt caaccagagt ttgtttggaa atctgcagat gtcgtccttc
      121 atgtttccat tgatcttgct tgtaatcatg ttgaccaaag taatagccat ggctctgaca
      181 aatgctgcag gtggtattgg tggggttttt gcgccttctc tgtttatggg tggcatgagt
      241 ggtttgtttt ttactcgcac gctcgatctt cttacaggaa ttcgaatgcc cgaaaacaac
      301 ttcgttctgg taggaatggc tggtgtaatg gcaggagtta tgcacgctcc tcttacggct
      361 atattcctga ttgccgaaat tacaggaggt tatcaattgc ttaccccttt aatgctgact
      421 gctgctattt cttttatcac catccaatat ttcgaacctc attccatcta tacaaagaag
      481 ctggccgaac gtggcgaact gattactcat cataaggata aagctgtact ctcgatgatg
      541 aaagttaccg atttgcttga aaccaatttc aaaactgttt ttcctgatgc taccttggga
      601 gaattcgtaa aagtagtagc aagttccgag cgtaatgttt tccctgtggt agataaagag
      661 aataattttt tgggagtagt tttcatcaac gacattcgac atattttgtt tcaacaggaa
      721 ctatatcaca agatcttcgt aaaagatttg atgtttatgc ccgatgtaaa agtaagcccc
      781 gacgatacta tggaagatgt ggctaaactg tttcaacaaa ccgcccacta caacattcct
      841 gtgataaaag atgggaaata tctgggcttc gtttccagag ccaatgtctt ttccaattat
      901 cgtaaattgc ttaaggagtt ttcggaagac tgatcatctg ttttctttgt tgttttatcg
      961 tagatttaat tttctgtttt taaaaaggat gtatatttgc tgccaaagtg aaccgaatgt
     1021 ttaattaaat atctaaatct aagtatatgt tcaagaatca tcccaaagga ttattggttt
     1081 tagccattac caacatgggc gagagatttg gctactacac catgttggct attcttacct
     1141 tgtatatgca ggccaagttt ggtttgactt cggccacaac cagcatgatt tatggaacct
     1201 ttctggctct tgtttatttc ctccccattt taggaggagt tatagctgat aaggcactgg
     1261 gttatgggaa aacgattctg ttgggcatcc tggtcatgtt tatcggatat ctgttgcttg
     1321 ctattccact cgaaagcaat gcagtaggat tggccatgat gattttttcg ctcgttctta
     1381 ttgctttggg tactggattt ttcaagggaa atttacaggc actcgtaggc aagctttacg
     1441 atgatccccg gtatggaaag aatagagata ttgcattttc gattttttat atgtttatca
     1501 atattggggc cttctttgct cccagcgcag caaattatat caataatacc gttttgaggt
     1561 cggccgattt tacctacgac gcttccatac ctaaaaacta tgtcattctc aatgatgaaa
     1621 agaatgccat tgataagaac acatttattc tgaaatatat aagccccgaa gaacaaaagc
     1681 tgtcgggaaa ggaattcgaa tccgtcttga acaaggcaaa aaaggaacat aaagcggttt
     1741 tcaccgatga tatcgaattt gcccttttca gattgaaagc cgctggcctt tatcaatata
     1801 ctcaatcggg caagattacc gattcctcca tgatgattag cagccatgaa tacgaattgt
     1861 tacaaagccg tgatcccaaa gatgctcagg acaaagccga tgtaataacc aaaatacaag
     1921 ctattcatgt aacagaccca aatttggggg gtggcccaaa ccccgacgat tttgctcgca
     1981 attttggcaa gcattatgtt agtcattcac tttccacctc ttataatctg gcctttggcg
     2041 tagcttgcat cagtttgatc atttccgttc tcatcttcct gctgttcaag aaaacatgga
     2101 aagcagtaga taaaacggta aaacaggaaa tcgccgaagc taagataaca ggcaaagagg
     2161 taaagatgat gccaaaagaa gaagtgagag aacgaatgat cgctctgttc ttggtatttt
     2221 tcgtggttat attcttctgg atgtcgttcc atcaaaatgg attaacgctt accttctttg
     2281 cccgcgatta taccatgagc aatattccgg ccgcatggac tatgctgttc aatctattcc
     2341 cactgctatc tctaattgta tttttatatg gcatttatct ttcgaccata ggattgttgg
     2401 gcagcaaacc caatagaaaa atcggaattt tacttgttct ttttggtatt gcaggcatgg
     2461 caggttatta ctttacaagt atttatggca tagataagat gaccaagata acccctcaaa
     2521 tcttccagca gttcaatccg atgtttatca tcattcttac gcctcttaca gtcgctttgt
     2581 ttggatatct tgccagtaaa gggaaagaac cctcagcacc ccgaaaaata ggtattggga
     2641 tgtttctggc agccgttggt tatgccatta tgatgatttg ctccataggt ttgcccgccc
     2701 cagccgaaat ttcgggtata tccgataaat tagtatctcc ctcatggctt atcggtactt
     2761 attttgtgct aactattgcc gaattattcc ttagccctat gggactgtcg ttcgtgtcga
     2821 aagttgcacc accccaatat agtgggatca tgcagggtgg ctggctggcc gctacggcca
     2881 tcggaaacct gctggtgggt gtagtgggta ccttctggga aaaacttcct ctggttggat
     2941 tttggtcaat cttgattgtc tgttgtctcc tttcgggcat tttcatcttc tcgatcatga
     3001 aaaagctcga aaccgctaca gagtaatttc ttctcagtcg aagaattatt taccacatga
     3061 attctgatgt atataaaccg gaattcgaaa agtgaaagta atgaaatatc cctggggtga
     3121 tgataatcct tattattctt attcgactta ttccaaacgg atttttggag gaaggattca
     3181 aaaactctct ataaaggcag gttttacttg cccaaaccgt gatggaacaa aaggaatagg
     3241 aggttgctat tactgtaata acgatgcatt caacccttct tattgtcgtt ctgacaagag
     3301 catcactcag caaatcgaag aaggaatagc ctttcatgcg tggagatacc ggagggctac
     3361 caaatatctg gcttattttc aagccttttc gaatacttat gcacctttgt ctcatttaca
     3421 gaaattatac ggcgaagccc tcagtcatcc cgcagtagtt ggtctggtaa tctctacccg
     3481 tcccgactgc ctgccagaag aaacactcat ttatttgtcc caactttcga aagaatattt
     3541 catacaggta gaaattggga tagaatcgtg ttatgacgcg actttgagtc gaataaaccg
     3601 tgggcatagt tttgatgata ccataaaagc cttggataac ctttccatga gaggtataat
     3661 gacaggagct catctcatct ttggtcttcc gggcgaaact atcgagatga tgctgaatga
     3721 agcccaaata gtctcgcaat tgcctctgac gagtattaaa cttcatcagc ttcagattat
     3781 cgagggttcg gtttgggcaa cagaatatcg ccttcgttcg gaaagtttta gacttttcga
     3841 acgcaatgaa tatgtggatt tcattataaa attcattgaa agacttcgcc cagaaatagt
     3901 agtagagcga ttatcggccg aagctcctcc gcgcttcgtg attgcacccg attggggttt
     3961 ggaacgtgcc gatgcttttc agcaagatat tcgagaaaag atgctgagct tgcaaaccta
     4021 tcaagggaaa cttttcaaat tcaatgaaaa ataggggaaa aaataaaatt ttctcgaatt
     4081 ttttgtagaa tattaaataa atatattaag tttgtatccg tattcataag tatcaacaaa
     4141 aatctaaaac tatgaaaaaa ttgtattctc ttatcgtcgc gatgttcctg attgtgggag
     4201 cttttgcgca aactccaccc tatactgaca atttcgagtc atatacggtg ggtgggtatc
     4261 tggctgctca aaatcctgac tggtggacta catggagtaa caccccgggt acgggtgaag
     4321 acgctatcat ttcgaatgct catgccaact caggcgctca atcagtactg gttgacgaaa
     4381 ctggcggctc tactgacctg attttgaaac tcggtaataa aaccgtaggc ctctattaca
     4441 taggttggta tgtctttgtt gataatggta aagcaggtta ttacaacatt caacatttcc
     4501 aatcaccagg tactgaatgg gccttcgagg tctattttaa tactaatgga agtggtacac
     4561 ttaaagcggg agggaatagc ataaccttta gctaccccaa agctacatgg tttaaggttt
     4621 atcatgaaat cgacctcgat aatgaccaga ttaaacttta tgtaaatgga actcaggttc
     4681 atcaatggcc attcagctat caagccagct ctactacggg cacaaaacaa ctgggagcag
     4741 ttgacttctt tgctggagct caaacaggcg aaacccccca gtactggttc gatgatattt
     4801 cattcgaact tgcacccgca cctctatatt cccagaattt cgatagctgg gctgtgggta
     4861 gctatgtagc cgtgaatgat cctgagtggt ttactacatg gagcaattcc cctggcactg
     4921 gagaagatgc tctgatagat aatgcttttt cccatagctc ttccaattct gcccgtgtcg
     4981 atgaaactga cggcgctacc gacctcatct taaaactgag taataaaact tctggtcatt
     5041 ataacctctc ctggtatatg tatatcgaaa acgggaaagc agggtattat aatattcagc
     5101 acttccaatc gcctggtacc gaatgggctt tcgaaatgta tttcaaagca aatggaacgg
     5161 gaaaattgct ggaaggaaac aacaccatta cctttactta tcctcaagct acatggttta
     5221 aagtagaaca cgatatcgat atagacaacg attggatcaa actctatgtg aataatgtat
     5281 tgatagaaga atggccattt agctatcaat cgggttcaac cacaggaacc aatcaacttg
     5341 gagctgttga tttctttgct ggggcagaac agggaagtgg tgaaacacca aaatatttct
     5401 tcgatgatgt agtgtttatg gcttatggag ccattcccga agccaacatc gaagtagatc
     5461 ctacgtcatt ggctgcctat ctcgaaccca attcaaccat tactgaacaa attaccatta
     5521 ccaacagtgg ttcactcgat ttgagttttg atgcactggt aattttgaat tcgggtaaaa
     5581 tggcaggcaa agcaaccgca actcctcaac cagctgtatt gaccaatgcc tttgcaggtc
     5641 ccgtactcaa tggaggagct ccttctaccg atgctactgc cgttttgcat tacgatggag
     5701 ataattattc tgctatagga tggagcagtg cacctgttac agttaccgtt gcggctatgt
     5761 tcccctacac catgactgct ccctatgccg gcatgaaaat caactcggta gatgtttata
     5821 tcaacgatct gaattcaagt ggcagcaatg agatgacagt gaagatctat ggaatggggt
     5881 taacatacga acccggcccg ctcatttatt ctcaaccttt cactcctatg gggggaggct
     5941 gggaacacat cgttcttact actcctgtct tggttaccgg tgaagatata tgggtaggct
     6001 atcaatttac acaaaacgaa accggaatct atattcctgg cacggatgcc ggtcctaatc
     6061 atccttatgg cgatttcctc agcacgggag tgggctggag ccacctgtcc aataatccca
     6121 gcctgcttta taactggaac atccgcgcca acctcaatgg cgatcctttc aataagtggc
     6181 ttaccattac tcctacctct ggaacaattg ctcctgccgg ctcacaaatt ctcgacgtaa
     6241 ctttcaattc taataatcaa cccgttggaa tctacgaagg aatcgtacgt ataatgagta
     6301 atgaccccga ttcgccttcg gttgatgtac tctgtacact ttcggtatca gtaggaatca
     6361 acgacatgaa caaagtagct gtattggttt atcccaatcc cgctcaagat tttatcaatg
     6421 tggctgctaa ccaaaaaatt accgaaatca gaattatgaa ttttgcagga caaaccctct
     6481 acaaaggcaa tgaaaaccat atcaatatta gcaatttgcc aaaaggaatc tattacctgc
     6541 aaaccattac ccacgaaggg atttccaata tcaaattcat taaaaactga gatcctttct
     6601 ctttgacttt gattttcacc gcccttgcta tatgccgggg cggttttttt atttttttca
     6661 ccaaaatttt acactccttt attacaatat tccagacgat aactcttttt tcttcatact
     6721 tttgcaaatg tttaactttc gacctgctta ttcgtggatc atttatcatt tcctatttta
     6781 atatgattat gaaatactgt aaaccagtct tattattcgt tctgtttatc ttgatattac
     6841 cgggttgcgg ggctcaggtt gacgagcaga aaaggaatct tatcctgatg gatcttttga
     6901 aacgctcggt ggaagaaggc cattttagcc ccgttgccat ggatgatcaa tattcgaaaa
     6961 aagcttataa tttatttatc gaacaactcg atcctctcaa aagattcttt accgaggatg
     7021 atctggctga aatgaaaaaa tatgagctga ggttagatga tgaattcaag aattatgata
     7081 ctgagttctt tgaattgact tataatatct tatcgaagcg gttgaaacag atcgaagatt
     7141 tttactccga gattttgaaa agacctttcg atttcggaaa ggaagaatac ctcgagatgg
     7201 accctgataa acgtcgatat cctaaaaatg atgaagaact taaaacagct tgggagcaaa
     7261 tgttaaaata tgaggtactc aattggctta c
//
LOCUS       DAOORU010000124         7212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_12741_length_7212_cov_6.374319, whole genome shotgun sequence.
ACCESSION   DAOORU010000124 DAOORU010000000
VERSION     DAOORU010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7212
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12741_length_7212_cov_6.374319"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /gene="sufD"
                     /locus_tag="PLF20_07935"
     CDS             complement(<1..1206)
                     /gene="sufD"
                     /locus_tag="PLF20_07935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HOX80966.1"
                     /translation="MEIVNQSLLDTSYWKEIFQQHRDAITHYDTPLLTKMRNEALENF
                     LSSGIPDLNHERWRNTDIRKFFRNDWNFLFQMPDNHGKIEDFFLCEVQGFDTLLITLF
                     NGWYTYKNSPLRILTDGTIIGGLRAALVEAPEIIERYLGRFADFRQNAFTSLCTAFAS
                     EGFFIYVPDGVQLQKPIQMVNVVNYSQPLFMQTRNLIIAGKGSSIQLLHCDDSNNNEP
                     AFTNSLTEIYVGEGASVDYYKLQNINDHSVIEHNAYIHQEKQSVCNTYSINLHGGLIR
                     NETYNHLAGHHAEGNHFGLYLMDKNQQADNLIFVDITASDCVSYELFKGVLDDYAHGV
                     FNGHILVHNDGQRTQAYQSNRNILLTDKALVNTKPFLEIYADDVKCSHGATIGQLDKE
                     AMFYLRSRGI"
     gene            complement(1225..1983)
                     /gene="sufC"
                     /locus_tag="PLF20_07940"
     CDS             complement(1225..1983)
                     /gene="sufC"
                     /locus_tag="PLF20_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HOX80967.1"
                     /translation="MLLQIKNLHVAIQGKEIIKGIDLVVNEGEIHAIMGPNGTGKSTL
                     ASVIAGREDYEVTEGEIVYKGKNLLEMPVEQRAREGIFLSFQYPVEIPGVSMANFMRT
                     ALNDIRKYKGLEPLSGSEFLLRMEEKKQLVEIQSQLTNRSVNEGFSGGEKKKNEIFQM
                     AMLEPTLAILDETDSGLDIDALRIVANGVNKLRRPDNAFIVITHYQRLLNYVIPDFVH
                     VLYDGKIVKSGKKELALELEEKGYEWIKKEVASL"
     gene            complement(2034..3482)
                     /gene="sufB"
                     /locus_tag="PLF20_07945"
     CDS             complement(2034..3482)
                     /gene="sufB"
                     /locus_tag="PLF20_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013815481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HOX80968.1"
                     /translation="MPNDKDDILNEITNSDYKYGFVTNIETEFAPKGLSEDIIRYISR
                     KKDEPDFLLDFRLKAYQRWKEMQHPQWAHLDIPPIDYQDIIYYAAPKVNKGPSSLDKV
                     DPELLSTFEKLGIPLEEQKRLTGVAVDAVMDSISVKTTFQETLNKLGIIFCSMSEAIR
                     HYPDLVKLYLGSVVPYADNFYAALNSAVFSDGSFVYIPKGVRCPVELSTYFRINAAKT
                     GQFERTLIVADEGAYVSYMEGCTAPMRDENQLHAAVVEIVALKDAEVKYSTVQNWYPG
                     DREGKGGIYNFVTKRGLCKGSYSKISWTQVETGSAITWKYPSCILQGDNSRGEFYSVT
                     VTNHYQQADTGTKMIHLGRNTSSIIISKGISAGFSNNSYRGLVRVAKKAENSRNYSQC
                     DSLLLGDKCGAHTFPYIQVENKSSIVEHEATTSKISDDQLFYCKQRGIDTESAIGLIV
                     NGYAKEVLKQLPMEFAAEAQKLLAISLEGSVG"
     gene            complement(3830..4126)
                     /locus_tag="PLF20_07950"
     CDS             complement(3830..4126)
                     /locus_tag="PLF20_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOX80969.1"
                     /translation="MNTLTKDLIFPKLGMGYNDLEPYIDAMTMEIHYTKHHRAYYDNL
                     MNAMVFPILGLDVWEHAYYLKYQNRRPDYIAAFLKIVNWKMIEENYRNALSMIR"
     gene            complement(4294..4944)
                     /locus_tag="PLF20_07955"
     CDS             complement(4294..4944)
                     /locus_tag="PLF20_07955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HOX80970.1"
                     /translation="MEGEYIFSSEIETYCQQHSEPETPLLSEIARYTWTTQAYPHMIS
                     SYIQGRLLSFISRILKPNCILEIGSFTGYSTLCLAEGLQPAGSLVTIEKNRELEEKLN
                     EFFSKSSNSKQIKLLIGDALRIIPDLQVTPDLIFIDADKEQYPQYLEICLPIMKKGSL
                     LIADNVLWGEKVIHHDSKSNKETHGIIRFNEMMTQKTELSVVMLPIRDGLTLAIRK"
     gene            complement(4946..6142)
                     /locus_tag="PLF20_07960"
     CDS             complement(4946..6142)
                     /locus_tag="PLF20_07960"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOX80971.1"
                     /translation="MQLLHQEIHSNVSHLAIFIKAGSRDEQPGKEGLAHFVEHNLFKG
                     TKKRNAFRVMNRIEAVGADFNAYTTKEETCLYASFLSPYYSRVVELFSDILLNSSFPE
                     KEIENEKEVVLNEIASCDENPDESILDEFENLIFDHHPLGHSILGTATTVSKFNQNDL
                     KAFIQAIYHPSRILLISAGNIPRQLLTKLIAHHFEKMPGINSLRQQNPPEVLPSFCAK
                     QYRQNHEAYCIMGIRTVAANHQHSYPLSLLNNILGGASMTSRLNLSLREKHGLTYCVE
                     SNYTSYSDSGYLYIYFQTSPRHIQSAIQLIYNEIKALKTKKLGSLQLSQAKRQYLGQA
                     FISWDIPQNRILNMGKQMLLQDKIYDPEDLSLIIGQISAKQLQEIAMEYFIPDRFNTL
                     LYYPDF"
     gene            6260..7114
                     /locus_tag="PLF20_07965"
     CDS             6260..7114
                     /locus_tag="PLF20_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOX80972.1"
                     /translation="MLKLLLAVILLGVVVTAAGNIMGKQSKNENKKEKKKKSFAKWLG
                     GGLGWALGGPIGGIIGFVLGSVYDGMQSGEFEYKGAQDVFPGQPAYTRRGDFTTSLLV
                     LSAAVMKADEKVMKSELDYVKDFLKRNFGEENAAEMLLVLRSILKQEIPVQAVCQQIR
                     ENMDYSIRLELLYFLFGLTLADKQISKAELDVLQSISLWMGITMADFLSIKALFIKDS
                     KAAYKILGVDENATNEEIKKAYRQLVINHHPDKVSHLGEDIQRAANEKLKQINAAYDE
                     IKKQRGFN"
BASE COUNT         2122 a         1412 c         1491 g         2187 t
ORIGIN      
        1 aatgcctctg gaacgaagat aaaacatggc ctccttatcg agctggccaa tagtagcacc
       61 atgactacat tttacatcat ctgcataaat ctcgagaaaa ggcttggtat tcaccaaagc
      121 cttatcggtt agaagaatat ttcggttact ttggtaagct tgtgtgcgtt ggccgtcgtt
      181 gtgaacgagg atatggccat tgaaaactcc atgtgcataa tcgtcgagca cacccttaaa
      241 taactcataa ctcacgcaat cggaagcggt aatatctaca aagatcagat tatcggcttg
      301 ctgattctta tccatgagat aaagaccgaa atgatttccc tcggcatgat ggccggccaa
      361 atgattgtaa gtttcattac ggataaggcc tccatgtaaa ttgatgctgt aagtattgca
      421 aacgctttgt ttttcctgat ggatataagc gttgtgttcg attacgctat gatcgttgat
      481 attttgtaat ttgtaataat caacactcgc cccttctcca acgtaaattt cggtaagaga
      541 gttggtaaag gcaggttcgt tattgttgga atcgtcacaa tgaagcaact gaatactgct
      601 tcccttcccg gcaatgatca gattacgagt ttgcataaac aatggctggg aataattcac
      661 tacattgacc atctgaatag gcttttgcaa ttgcacacca tcgggcacat aaatgaagaa
      721 tccttctgaa gcaaaggcgg tgcaaagaga agtgaaggcg ttctgtcgaa aatcggcaaa
      781 gcggccgaga tatctttcga tgatttcagg agcttccacc agggctgccc tcaggccgcc
      841 aataatggtc ccatcggtaa gaatccgtaa gggagaattc ttataagtgt accagccatt
      901 gaaaagggta ataagtaagg tatcgaatcc ttgcacttca cacaggaaga aatcttcgat
      961 cttaccatga ttatcgggca tttgaaataa aaaattccaa tcattcctaa aaaattttct
     1021 aatatcggtg tttcgccagc gttcgtggtt tagatcggga atacctgagg ataaaaaatt
     1081 ctcgagagcc tcattgcgca tttttgtgag cagaggagta tcgtaatgcg taattgcatc
     1141 cctgtgttgt tgaaagattt ctttccaata tgaagtatcg agtaacgatt gatttacgat
     1201 ttccatatca tcaatgcttg aaaattataa ggaagcaact tcttttttta tccattcgta
     1261 gcctttttct tcgagttcga gggcgagttc cttttttcct gattttacga tttttccatc
     1321 gtaaagcaca tggacaaaat cgggaattac ataattgagg agtcgctggt agtgtgtaat
     1381 cacgataaag gcattatcag ggcgtcgcaa tttattcacg ccattggcca ctatacgcaa
     1441 agcatcgata tcgaggcctg agtcggtttc atctaaaata gctaatgtag gttcgagcat
     1501 ggccatttga aaaatctcgt tctttttttt ctctcccccg ctaaaccctt cattaacact
     1561 acgattggta agttgagatt gaatctccac cagttgtttc ttttcttcca tcctcaacag
     1621 aaattccgaa ccactcaagg gttcgaggcc tttgtatttg cgaatgtcat tcaaggctgt
     1681 gcgcataaaa ttagccatac ttacgccagg tatctcaacg ggatattgaa aactcaggaa
     1741 aatgccttca cgtgcccgct gttcgacagg catctcgaga agattcttgc ccttgtacac
     1801 gatttctcct tcggtaactt cataatcttc acggcctgca atcaccgaag ccaaagtgct
     1861 cttccccgtt ccattaggcc ccatgatggc atgaatctca ccttcgttca ccactaaatc
     1921 tattcccttt ataatttctt tcccctgaat ggcaacatga agatttttaa tctgtaatag
     1981 catgatattt ctgtttgatt ttttcgattg ataaaattat tcgataagac acgctaacca
     2041 acgcttcctt cgagactgat agcgagcaat ttctgagctt cggcggcaaa ttccatgggt
     2101 aattgtttca atacttcctt ggcatatcca tttacaatca atccaatggc cgattccgta
     2161 tctatgcctc tttgcttgca ataaaacagc tgatcgtctg aaattttcga tgtagtagcc
     2221 tcgtgctcaa cgatggatga tttattttcg acctgaatat aaggaaaagt atgtgcaccg
     2281 catttatcac ccaacaacaa tgaatcgcat tgactgtaat ttcgggaatt ttcggctttc
     2341 tttgcaaccc ttaccaatcc tcgatagctg ttattgctaa aacctgccga tattcccttg
     2401 ctgatgataa tgctagacgt attgcggcca agatgaatca tcttagtccc agtgtcggct
     2461 tgctgataat ggttagtaac ggttacggaa taaaactcgc ctcttgaatt atccccctga
     2521 aggatgcagc ttggatattt ccaggtaatg gccgacccgg tttcgacttg tgtccatgat
     2581 atcttcgaat aacttccctt gcataaacca cgtttggtaa caaagttata tatgcccccc
     2641 tttccttccc tgtcgccagg ataccagttc tgaaccgtcg aatacttcac ttcggcatct
     2701 tttaatgcta caatttcgac tacggcagca tgaagctgat tctcatcgcg cataggagcc
     2761 gtacatcctt ccatatagct tacataggct ccttcatcgg ccacaatcag cgtgcgctcg
     2821 aactgacccg ttttggctgc attgattctg aagtaagtag aaagttcgac aggacaacgc
     2881 acgcctttag gaatataaac gaatgaaccg tcggaaaaaa ctgccgaatt caaagccgca
     2941 taaaaattat cggcataggg tacaacacta cccagatata actttaccag atcgggataa
     3001 tgtcttattg cttcactcat cgaacaaaag atgatgccga gtttgttgag ggtttcctga
     3061 aatgtcgttt ttaccgagat gctatccatt acggcatcaa cagccacgcc agtcaggcgc
     3121 ttctgttcct cgagaggaat accaagtttt tcgaaagtac ttaacaattc gggatcaact
     3181 ttatccaaag aagacgggcc tttattgact ttaggagcag cataatatat aatatcctgg
     3241 taatcaatgg gtggtatatc taaatgagcc cattgtggat gctgcatttc cttccatcgc
     3301 tggtaagcct tcaacctgaa atcgagtaaa aaatcgggct catccttttt tcgagaaata
     3361 taacgaataa tatcttccga aagccctttg ggagcaaact cggtttctat attggtaacg
     3421 aacccgtatt tataatcgga atttgttatt tcattcaaaa tatcgtcctt gtcgttaggc
     3481 ataactaaaa tttaattcct ctcgcgtttg tatttctgtc gaattcgcaa gaactatcat
     3541 gaattgacga tacatttatt tttaaaaaat cacgtcgagt ttccaaggaa tgatctgcaa
     3601 aattagacaa ctttcggcat ttctggcttt aaaaatgagg ttcaaataaa aacaagctaa
     3661 gtaattattc aaggcacatc atctattatt gattctgtct ttttataaaa ttttaaaccc
     3721 tttcggtgct tttatctatc atgtcaatct cgatcaaaaa atgtactgac tcccaaccac
     3781 tttcatatcg agaatcaatc actaaaatca aaaaacattc aaaaatcaat tatctgatca
     3841 tggaaagagc atttcggtaa ttttcttcga tcattttcca attaactatt ttcaagaagg
     3901 cggcaatata atctggacgg cggttctggt attttaaata atatgcatgt tcccaaacat
     3961 cgaggcccag gatgggaaaa accatggcat tcatcaaatt atcataatag gcacgatgat
     4021 gcttggtata atgaatttcc atggtcatcg cgtcgatata aggttcaagg tcattatacc
     4081 ccatccctaa tttcggaaaa attaaatctt ttgtcagtgt attcatgatt ttattagctt
     4141 attaagaata tttctaaata aacattgaaa aacaagaaag gttcacatag caacgataaa
     4201 atcgaaagat gaccggtaaa attctggata atgatgtttc aatcaatgat ggagcggagt
     4261 aaaatatctc gatctatcat aaggaacaga aaattatttt cttatcgcca gagtcaagcc
     4321 gtctctaata gggagcatca ccacagaaag ttcggttttt tgcgtcatca tctcattgaa
     4381 tcgaataatg ccatgggttt ctttattgga ttttgaatcg tgatggatga ctttttctcc
     4441 ccaaagtacg ttgtcggcta taagcagaga gccttttttc ataataggca agcatatttc
     4501 aaggtattgt gggtattgtt ccttgtcggc atcgataaaa atcagatctg gagtaacttg
     4561 caaatcgggg atgattcgca gagcatcccc gatgagaagc ttaatctgtt tcgaattaga
     4621 tgatttcgaa aaaaattcat ttagtttttc ctcgagttcc cgattttttt cgatggtaac
     4681 caatgaaccc gcgggttgaa gcccttcggc gaggcataag gtagagtatc ccgtaaatga
     4741 accgatttcg agaatgcaat tcggtttgag tatacggcta atgaagctga gcaaacgccc
     4801 ctggatatag ctcgaaatca tgtgaggata ggcctgagtt gtccatgtgt atcttgcaat
     4861 ttcgctcagc aaaggggttt cgggctcaga atgttgctgg cagtatgttt caatttcact
     4921 cgaaaaaata tattctcctt ccatattaaa aatcaggata atacaataaa gtattaaaac
     4981 gatcgggaat aaaatattcc atggcaattt cctggagttg tttggctgaa atctggccga
     5041 tgatcaacga tagatcttcg gggtcataga ttttatcttg caaaagcatt tgttttccca
     5101 tattgaggat acggttttga gggatatccc atgatataaa ggcttgtcct aagtattgac
     5161 gcttagcctg cgataactgc aaacttccca attttttggt tttaagggct ttgatttcat
     5221 tataaataag ttggatagcg gattgaatgt gtcgtggcga ggtttggaag taaatataaa
     5281 ggtatcccga gtcggaataa gaggtgtaat tagattctac acaataggta agtccatgct
     5341 tttctcttag cgaaaggttg aggcgtgaag tcatggaggc tccacccagg atattgttga
     5401 gaaggctcaa aggatagcta tgttgatggt ttgctgccac agtacgaata cccatgatgc
     5461 aataggcttc gtgattttgc ctgtattgtt tcgcacagaa tgaaggaaga acttcgggag
     5521 gattttgttg acggagagag tttattccag gcattttttc gaaatgatgt gcaattaatt
     5581 ttgtaagtaa ttggcgtgga atgtttcctg ccgaaattag taaaatacgc gaagggtgat
     5641 atatagcttg aatgaaggcc ttcaaatcgt tttgattgaa ttttgaaacc gttgttgcag
     5701 taccgaggat agaatgcccc agtgggtgat ggtcgaaaat gagattttca aattcatcga
     5761 gtatgctttc atcgggattt tcgtcgcagg aggcgatctc attcaaaact acttcttttt
     5821 cgttttcgat ttctttttct ggaaaggaag aattaaggag aatgtcgctg aaaagttcga
     5881 ctacgcgaga ataataaggc gaaaggaagg aagcataaag gcaagtttct tcttttgtag
     5941 tataggcatt gaagtcagct ccgactgcct cgattcggtt cattacccga aaagcattcc
     6001 gtttttttgt gcccttgaag agattatgtt ctacaaaatg ggccagacct tcttttccgg
     6061 gttgttcatc gcgcgaacct gctttgatga agatagccaa gtgagaaaca ttcgaatgaa
     6121 tttcctgatg cagcagctgc attccattaa gaaattcatg aaaccagtaa ttcataacat
     6181 aaatttacac cacaaaatta gagatatttg caggtgagaa atggtgatta tagataatga
     6241 ttaattttgc gacctgaaaa tgttgaaatt attattagcg gtcattttgc tgggtgttgt
     6301 agttacagca gcaggtaaca ttatgggaaa acaaagtaag aatgagaaca agaaggaaaa
     6361 gaagaagaaa agctttgcca aatggctcgg tgggggcctg ggttgggcat taggggggcc
     6421 aataggtgga atcataggtt ttgttttggg ttcggtatat gatggcatgc aatcgggtga
     6481 gttcgaatat aaaggagcgc aggatgtatt cccggggcaa cctgcttata ctcgacgtgg
     6541 cgactttact accagtcttc tggtgctttc ggcggcagtt atgaaagccg atgaaaaagt
     6601 aatgaaatcg gaactcgact atgtaaaaga tttcctgaaa cgaaattttg gtgaagagaa
     6661 tgctgctgaa atgcttctgg tattgcgcag tattttgaaa caggagattc ctgttcaagc
     6721 cgtatgccaa cagattcgtg aaaacatgga ttatagcatc cgcctcgaat tgttatattt
     6781 tctctttgga cttactcttg ccgataagca aattagcaag gccgaactcg atgttttaca
     6841 atcgattagc ctttggatgg gaattactat ggccgacttc ctttccatca aagcactttt
     6901 tataaaggac tcaaaggctg cttacaaaat tcttggagtc gacgaaaatg ctaccaatga
     6961 agagattaag aaggcttatc ggcaactggt cattaatcat catcccgaca aggtttcgca
     7021 tttgggagaa gacattcaaa gagctgccaa cgaaaagttg aagcagatca atgcggctta
     7081 tgacgaaatc aagaaacaaa gaggatttaa ttgagtatca ccctcctcat gaggatttat
     7141 tcattcctcc ttgtatacct gacttcgaca atgcgtcacc ctaaaaattt tttcaaaatt
     7201 tttcatcgaa aa
//
LOCUS       DAOORU010000125         6993 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_13243_length_6993_cov_5.194581, whole genome shotgun sequence.
ACCESSION   DAOORU010000125 DAOORU010000000
VERSION     DAOORU010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6993)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13243_length_6993_cov_5.194581"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1324)
                     /gene="glyA"
                     /locus_tag="PLF20_07970"
     CDS             complement(44..1324)
                     /gene="glyA"
                     /locus_tag="PLF20_07970"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOX80973.1"
                     /translation="MIRDIQVFDIIEKERLRQTEGIELIASENFVSQQVLEAMGSVMT
                     NKYAEGYPGRRYYGGCQFVDMTEQLAIDRLKELFGAEYANVQLHSGAQANMAVFLACL
                     KPGDTFMGLDLAHGGHLSHGSPVNSSGILYRPLAYRVDEETGTIDYDKMEELALLEKP
                     KLIIAGASAYSRDWDFARMRKIADKIGAILMADIAHPAGLIARGLLNDPLPYCHIVTS
                     TTHKTLRGPRGGIILMGKDFENPWGQKTPKGEIKMMSQILDSAVFPGVQGGPLEHVIA
                     AKAVAFGEALTPEYFEYIKQVKKNAEVMAKVFIEKGYKVISGGTDNHLMLIDLRPKFP
                     EITGKQVENTLVKANITLNKNMVPFDSRSPFQTSGVRVGTPAITTRGLKEDQLPSIVE
                     MIDEVISHIDDDSVIEKVGHKVKEMMSAYPLFAY"
     gene            complement(1346..2392)
                     /locus_tag="PLF20_07975"
     CDS             complement(1346..2392)
                     /locus_tag="PLF20_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOX80974.1"
                     /translation="MNEQIINNTIPKSAVPLTPEDFVSTEMVRWCPGCGNHSILKSME
                     KVFPVIGYKKENYVLVSGIGCSSRFPYYVNTYGFHGIHGRATAIASGVKISNPTLSTW
                     IITGDGDSMAIGGNHFIHLIRRNIDVNLLLFNNQIYGLTKGQYSPTSPLGAITKTSPY
                     GTIEHPFTPGELVLGAEGTFFARGIDVLPKLLTDIMFEAARHDGTSVVEVLQNCVIFN
                     DGAYEYIANRQHRDDRTILLEHGKPMLFGKNKDKGLRWNLNSLEVITLGENGMGINDV
                     LVHDAHNPDPGLHLSLAKMNWPEYPVALGVIRAAKFPTYDDLMEEQIRKIKAKSPIKC
                     MDDLLNSGDTWEVE"
     gene            complement(2405..4243)
                     /locus_tag="PLF20_07980"
     CDS             complement(2405..4243)
                     /locus_tag="PLF20_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOX80975.1"
                     /translation="MEEYQNQTEVKDDIVIRFSGDSGDGMQLTGTLFSETAAFTGNDL
                     LTFPDFPAEIRAPQNTLAGVSGFQLHFGSKKIYNSGDLCDILVVMNPASLKANLKWAR
                     KGATIIVDEDNFEDKAIQKAGYTSNPLTDGSLNSYVVIKVPMSRLAQEAVKDLGVDPK
                     SAERSKNMFALGMLLYMFNKPLDHAYHFIEKKFSKKSLVVEMNKRVLKAGYDYADTVE
                     ILPSRIHVPPAKMPKGKYRNITGNIATAWGLMAASEKSARPLFLGSYPITPASEILIE
                     LAKHRNLGVKTFQAEDEIAGINSAIGASYAGSIGVTSTSGPGLSLKVEALGLAVMTEL
                     PLVVVDVQRAGPSTGMPTKPEQSDLNLALFGRNGEAPLIILSASSPADCFYAAFEAAK
                     LSLEHMTPAILLSDGYIGLGSELFRIPKMADLPEIKPPIAQPNTPYKPYARDPETLVR
                     KWAIPGTEGLRHRIGGLEKTNIDGLVSTDPINHETMVKLRAEKVKRIENFIPLQEVYG
                     ESEGRLLVVSWGGTYGAVRNAVDHCQKDGYKIAHAHFRYINPLPRNTAEIFKRYEKVL
                     VCELNAGQFANYLRMNFSEVKYTQYNKIQGLPFMVSELYKAIISNF"
     gene            complement(4645..5226)
                     /locus_tag="PLF20_07985"
     CDS             complement(4645..5226)
                     /locus_tag="PLF20_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOX80976.1"
                     /translation="MKKLILLTSFVIISFLCVNAQMKINPSVGIGIAVPMGDFGDAVG
                     LGYGASVGADFKIKPHIKPELIASLGYYMFSSKEQKYTKSSMTGGEYGFKTKANTFYI
                     GAGAKYVTLSGFYGAALISFHNFTNKVEYNYPDYLPLEDETISKDFTRLGFTPIVGYQ
                     FKLAALKLEVYVRYSIISDYNNFGAGASLKFSL"
     gene            5789..5959
                     /locus_tag="PLF20_07990"
     CDS             5789..5959
                     /locus_tag="PLF20_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX80977.1"
                     /translation="MAYVITEDCIACGTCIDECPVEAISEGDIYKIDPDMCTDCGSCA
                     EVCPTEAIHPAE"
     gene            6636..6968
                     /locus_tag="PLF20_07995"
     CDS             6636..6968
                     /locus_tag="PLF20_07995"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HOX80978.1"
                     /translation="MSRIRNWRKGGHCFVRYADDCNIFVCSHEAGERVLRSVSSFIEG
                     KLKLKVNTEKSKVCLSREAKFLGYGIEIDGTLTISGISIKRFKDKIHRLTQRNRGRSI
                     AQHRLFVS"
BASE COUNT         1969 a         1397 c         1551 g         2076 t
ORIGIN      
        1 agcttatcac cgagagtctc ttcatacagc gattacgcaa gcatcaataa gcgaataaag
       61 ggtaagcgct catcatttct ttaaccttat ggccgacctt ttcgataaca gaatcatcgt
      121 cgatatgaga gataacttca tctatcattt cgacaatcga aggcagttgg tcttctttga
      181 ggcctcgagt ggttatggca ggtgttccga ctcttacacc ggatgtctgg aaaggtgaac
      241 ggctatcgaa tggtaccata ttcttattca aggtaatatt agccttaaca agtgtattct
      301 cgacctgttt tccggtgatt tctgggaatt ttggtctgag gtcgataagc atgaggtggt
      361 tatcggtgcc tccactgatc actttatacc ctttttcgat gaataccttt gccattactt
      421 cggcgttttt cttcacctgt ttgatatatt cgaaatattc gggagtaaga gcttcaccaa
      481 aggccacggc ttttgctgca ataacatgtt caagggggcc gccttgcacg ccggggaaaa
      541 ctgcagagtc gagaatttgc gacatcatct ttatttcccc tttaggtgtt ttttgtcccc
      601 agggattttc gaaatccttg cccatgagga taattccgcc acgagggcca cgcagggttt
      661 tatgcgtggt agatgtaaca atgtggcagt atggcagggg atcgttgagc aaacctcggg
      721 caatgagtcc cgcgggatgg gcaatatcag ccattaggat agctcctatt ttatcggcaa
      781 tcttcctcat tcgtgcaaaa tcccaatcgc gcgaataagc cgatgccccg gcaatgatga
      841 gttttggctt ttcgagcaag gccagctctt ccattttgtc ataatctatt gtaccggttt
      901 cttcatctac cctataagcc agtgggcgat ataagattcc agaagaattt accggcgaac
      961 catgcgaaag atggcctcca tgcgccaggt ctaatcccat aaaggtatct ccgggtttga
     1021 ggcaggccag aaagactgcc atattggcct gtgctccact atgcagttga acgttggcat
     1081 attcagctcc aaagagttct ttcaagcggt cgatggcaag ttgttcggtc atatctacaa
     1141 attggcaacc tccataatac ctcctgcccg gataaccttc ggcatatttg ttggtcatta
     1201 ccgaacccat tgcttccaga acctgctggc tgacaaaatt ttcagaagca atcaattcaa
     1261 tgccttcggt ttgacgaaga cgttcctttt caataatgtc gaaaacctga atgtctctta
     1321 tcattgctta aaattatttt gatgtttatt caacttccca tgtgtcgccg ctattcaaga
     1381 ggtcgtccat gcatttaatc ggactttttg ccttgatttt tcgaatttgt tcttccatca
     1441 ggtcgtcata agtaggaaat ttcgcagctc taataactcc caaagctact ggatattcag
     1501 gccagttcat tttagcaagg ctcagatgca aaccaggatc ggggttatga gcatcatgca
     1561 ccaatacatc gttgattccc atcccatttt cacccaatgt aatcacttcg agactattta
     1621 gattccatct gagcccttta tccttatttt ttccgaaaag cataggtttg ccatgttcga
     1681 gtagaatggt tctatcgtcg cgatgttggc ggttggcaat gtactcgtat gcgccgtcat
     1741 tgaaaattac gcaattttga aggacttcga ccacagaagt tccatcatga cgagcggctt
     1801 cgaacatgat atcagtaagg agttttggca aaacatcaat tccgcgggca aaaaaggttc
     1861 cctctgctcc caagacgagt tcgcctggag tgaatggatg ttcgatcgta ccgtagggag
     1921 aagtctttgt tatggcgccc agaggtgatg taggagaata ttgtcctttg gtgaggccat
     1981 aaatctgatt gttgaacaga aggagattga catctatatt gcgccggata agatggataa
     2041 agtgatttcc accaatagcc atagaatcgc catcgccagt aatgatccat gtgcttaggg
     2101 taggattgct aattttaact cccgaagcta tagccgtagc ccggccatga ataccgtgaa
     2161 aaccataagt attcacataa tagggaaatc tcgaagagca gcctatacct gaaaccagaa
     2221 cataattttc tttcttgtaa cctattacgg gaaaaacttt ttccatggat ttcagtatgg
     2281 aatgattacc gcagccaggg caccatctca ccatctcggt gctaacgaaa tcttcaggcg
     2341 taagagggac ggctgattta ggaatggtat tattgattat ttgttcgttc atgattcttt
     2401 cttcttaaaa atttgatatg attgctttat agagctcact caccatgaag ggaaggcctt
     2461 gaatcttatt gtattgtgta tattttactt ccgagaaatt cattctcagg tagttggcaa
     2521 attgaccggc attgagttcg cataccagaa ctttttcgta acgtttgaaa atttctgctg
     2581 tgttgcgagg aagaggatta atgtaacgga aatgtgcatg agctattttg taaccatctt
     2641 tttggcagtg atcgacggca tttcgcacgg caccataagt tccgccccag ctaactacta
     2701 aaaggcgtcc ttcggactct ccgtaaactt cctgcaaggg tataaagttt tcgatacgtt
     2761 ttactttttc ggcacgtaat tttaccatgg tttcgtgatt gataggatcg gtcgagacca
     2821 ggccatctat gttggttttt tcgaggcctc ctatgcggtg acggagtcct tccgtccccg
     2881 gtatagccca tttacgaacg agggtttcgg ggtcgcgggc ataaggttta tacggtgtat
     2941 tgggttgggc tatgggaggt tttatttcgg gaaggtcggc cattttgggg attcggaaaa
     3001 gctccgaacc aaggccaata tagccatcac ttaataagat agctggagtc atgtgttcaa
     3061 gggaaagttt ggcagcttcg aatgctgcat agaagcagtc ggccggagag gaagcagaga
     3121 gtatgataag cggggcttcg ccattgcgtc cgaaaagtgc aaggttcaag tcggattgtt
     3181 cgggtttggt aggcattcct gtggaaggtc cggcacgctg tacatccacc acaacaagtg
     3241 gaagctctgt catgacggcc aggccaagtg cttcgacctt gagcgacaag ccggggcccg
     3301 aagttgaagt tactcctatt gacccggcat aactggctcc aattgctgag ttaatcccgg
     3361 ctatttcatc ttcagcctga aaggtcttta ctcctaaatt gcgatgtttt gcaagctcga
     3421 tgagtatttc agaagcaggg gtaataggat atgaacctaa aaaaagtggc cgagccgatt
     3481 tttcggaagc agccatcaat ccccaagcag tggctatatt tcccgtaata ttgcgatatt
     3541 ttcctttggg cattttggcc ggaggaacgt ggatacgaga gggtaaaatc tctacggtat
     3601 cggcataatc ataacctgct ttcaaaacac gtttgttcat ttcgaccact aaagatttct
     3661 tgctgaattt tttctcgatg aaatgataag catgatccag gggtttattg aacatatata
     3721 gcaacatacc gagagcgaac atgtttttgg agcgttccgc cgatttggga tccacaccaa
     3781 ggtctttcac ggcttcttgc gccagccggc tcatgggtac cttgattacc acgtaagaat
     3841 tcaaactgcc atccgtcaag ggattgctcg tgtagccggc tttttgaatg gctttgtcct
     3901 cgaagttgtc ttcgtcaacg ataatagtag ctcctttcct tgcccatttc aggtttgcct
     3961 tgagagaagc aggattcatg actacaagta tatcacaaag atcacccgaa ttataaatct
     4021 tcttcgaacc aaaatgtaac tgaaaacccg aaactcccgc aagagtgttt tgaggtgccc
     4081 tgatctcggc cggaaaatcc ggaaaagtta aaagatcatt gcccgtaaac gccgcagtct
     4141 ccgaaaataa agtacccgtc aactgcatac catcaccaga atcaccggaa aaacgtatta
     4201 ctatatcgtc ttttacctcg gtttgatttt gatactcttc catatacatg acttttgaat
     4261 gttgatgcaa aggtatggct tatttatcaa ttgccaatta caatttattt cgtaatttgt
     4321 tatgagtctc ttaacagaga atcgaactct ccgaggagcc tgttaaaata tctcatttta
     4381 tatgtatttt acaagaaacg gaaatgtctt gtaaacgaag ataaataatt caaaatagaa
     4441 agggaataat ccattttttc gaatgtgtat agcaaaaaaa ccagctcgat atttagctcg
     4501 gaggaacgaa ccaaaaaccg aagctggctt ttgaaattga atatgcttac ttaattgagt
     4561 ttaaagcttg aacaaaacca tacctgaaat ctgttgaaaa ttagaagggt tataacaaca
     4621 aaggttgttg ttaatgattt agacttagag tgaaaatttc aaagatgcac ctgcgccgaa
     4681 gttgttgtag tcggatatga tggaatatct aacataaact tcgagtttta aggcagcaag
     4741 cttgaactga tagcccacaa tgggggtgaa acccaaacgt gtgaaatctt tacttatggt
     4801 ttcatcttcc aaaggtaggt agtcggggta gttatactcc accttgttgg taaaattatg
     4861 aaacgaaatc agtgcagcac cataaaaacc acttagagtt acatatttgg ctcctgctcc
     4921 aatataaaac gtatttgctt tcgttttgaa gccatattct ccacctgtca ttgagctttt
     4981 agtgtacttt tgttctttcg aagagaacat gtagtaaccc aagctggcaa tcagttcagg
     5041 cttgatatgc ggtttaattt taaagtcggc ccctacggaa gcgccataac ctaaacctac
     5101 agcatcacca aaatcaccca tggggacggc aatacctatt cctactgatg gattaatctt
     5161 catttgtgca tttacacaca aaaatgaaat gattacaaag ctggttaata gaattaattt
     5221 tttcatagta attgattttt aaatttttaa gttattgatt tagatgaatg aaacgagaaa
     5281 gatggttgtt ttttaaattt atatggttct attcatttgc tttaaactct ttgaagattt
     5341 ttttaagtcg catcataccc ttcttccctt cagggtggca aggagtattg ataagacttg
     5401 tcattgatag aacactcaga tggttttagt gttattctta tcatttttca ggatgatttt
     5461 aattcaatac aaatgtatgt aaacaaatca cgagatggca aaattttaca ttgaaaggta
     5521 aaatttattt tgtggtttct tcttaatgta catatcttta tcactattaa cgcattatac
     5581 atttaaaccc agcatattta aactcaaatt gccccaatcg atgacctata gctgttgcgt
     5641 atcgttgcaa taaatcgact attccatttt tagtcggtaa ttatgtacga atataggtat
     5701 tttttagaat caaaataaat tcagagagat aaatgagtat taaatgaaaa gatataattt
     5761 tgccaagtca attgaaaaat ttaaaatcat ggcttacgta attactgaag attgtattgc
     5821 ctgtggcacg tgcattgacg aatgccccgt cgaagcaatc agcgaaggtg atatttacaa
     5881 aatcgatccc gacatgtgca ccgattgtgg tagttgtgcc gaggtttgcc caacagaagc
     5941 gattcatccg gctgaataac tggcaattta aaatttcaat caggaaccac cgtactcttg
     6001 cggtggtttt tttttatgac gggcatacat tactacttgc aagatgaaag ttcttgtaca
     6061 cgccgagttg ggaggaggtg ttagcgaatg gaagggtgta agcgggattg atgggatggg
     6121 agtaggagaa tttgtcgaat ggtacacccg taatgggggc accttacgtc actccttact
     6181 gagtggcaac tatttgccac aaggtatcaa attcgtagaa atcactaagc ctaacggagg
     6241 gttatgcaag ttatgtattt ccacgatcat cgaccgagtc atccaacaag cgatagccca
     6301 ggttttaagt ccgatatacg agaagacgtt ttccgattat agttatggtt ttcgacccaa
     6361 acgtaatgat cgacaggctg tacgcaaggc gagtgaatat gtagttatgg ggagggaaat
     6421 tgttgtagat atcgacttaa agaacttctt tgacgttata aatcacgatc gtttgatgta
     6481 ccgattaagc gaaactatcg gggacaaggt actacttaag ctgatacgca ggtatcttcg
     6541 aagcgggatc atggcagatg gagtgttgaa ccaatgcact gaaggtactc tgcaaggcag
     6601 tccgctctcg ccattactgt ctaacatagt gttggatgag ccggataagg aattggagaa
     6661 aagggggaca ctgttttgtt cgatatgctg acgattgtaa tatttttgta tgcagccatg
     6721 aggcaggtga aagggtattg cggtcggtaa gcagttttat cgaaggcaaa ttaaagctca
     6781 aggtaaatac tgaaaagagt aaagtttgcc ttagccgaga agccaaattt ttgggatacg
     6841 gcattgaaat agatgggacg ctcaccatta gcggaataag tatcaagcgt ttcaaggaca
     6901 agatacacag gttaactcaa cgtaatcgag ggcgaagcat agcccagcac aggttatttg
     6961 tgagctgaat cctgtattac gaggctggct gat
//
LOCUS       DAOORU010000126         6935 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_13394_length_6935_cov_4.398837, whole genome shotgun sequence.
ACCESSION   DAOORU010000126 DAOORU010000000
VERSION     DAOORU010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13394_length_6935_cov_4.398837"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..345
                     /gene="rpsN"
                     /locus_tag="PLF20_08000"
     CDS             76..345
                     /gene="rpsN"
                     /locus_tag="PLF20_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOX80979.1"
                     /translation="MAKESMKAREVKRKMLNEKYAAKREALKKAGDYVALQKLPRNAS
                     PVRLHNRCKITGRPKGYMRDFGLSRINFREMASAGLIPGVKKASW"
     gene            377..775
                     /gene="rpsH"
                     /locus_tag="PLF20_08005"
     CDS             377..775
                     /gene="rpsH"
                     /locus_tag="PLF20_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004357709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOX80980.1"
                     /translation="MTTDPIADYLTRLRNAIHANHRIVEIPSSTIKKSMTQILFEKGY
                     IINYKFEDDKFPSTIKIALKYHPVTKACAIKELKRISRPGRRIYTSADKMPRVLNGLG
                     IAIISTSQGLMTDKEARKLNIGGEVICYIS"
     gene            791..1351
                     /gene="rplF"
                     /locus_tag="PLF20_08010"
     CDS             791..1351
                     /gene="rplF"
                     /locus_tag="PLF20_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOX80981.1"
                     /translation="MSRIGKKPIEIPSGVKVSLSPDHFITVQGKLGELCQKIDPAIKV
                     NIEGGEIRLEIDNPDDKNLRAKHGLYRSLINNMVIGVYEGFTIQQEIVGVGYKVAVSG
                     QLLEFSLGYSHSLFLELPPEIKAEAVTVRGKNPILILKSHDKQLLGMVARKIRSFRPP
                     EPYKGKGIRFVGEVVRRKAGKSASSK"
     gene            1374..1736
                     /gene="rplR"
                     /locus_tag="PLF20_08015"
     CDS             1374..1736
                     /gene="rplR"
                     /locus_tag="PLF20_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOX80982.1"
                     /translation="MSTKNRKLKRRQRIKTSIRKRLRGTPERPRLTVYRSNSQIYAQI
                     VDDTQGKTLIATSSLQKDFSREKMTKTQQARIVGVEIARKALEAGITAVVFDRNGFLY
                     HGRVKALADAARENGLIF"
     gene            1746..2264
                     /gene="rpsE"
                     /locus_tag="PLF20_08020"
     CDS             1746..2264
                     /gene="rpsE"
                     /locus_tag="PLF20_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOX80983.1"
                     /translation="MANANIRRVKSSEIEFKDRLVSIQRVTKVTKGGRAFSFSAIVVV
                     GNENGVVGYGLGKAKEVTAAISKGIDDAKKNLIRVPIIKGTIPHAQEGKYSGAVVFLK
                     PAAPGTGVIAGGAMRAVLESVGIKDVLAKSKGSSNPHSVVKATINALLQLRDPYTVAQ
                     LRGVDLDTVFNG"
     gene            2288..2467
                     /gene="rpmD"
                     /locus_tag="PLF20_08025"
     CDS             2288..2467
                     /gene="rpmD"
                     /locus_tag="PLF20_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOX80984.1"
                     /translation="MKRIRIKQVRSWIGKPIDQKRTLKALGLGRLNRTVEHTATPQIL
                     GMVRKVAHLVSVEEI"
     gene            2504..2950
                     /gene="rplO"
                     /locus_tag="PLF20_08030"
     CDS             2504..2950
                     /gene="rplO"
                     /locus_tag="PLF20_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOX80985.1"
                     /translation="MDLSNLKPAKGSVKSKRRLGRGQGSGRGGTSTRGNKGAQSRSGN
                     KRKIGFEGGQMPLHRRVPKGGFKNFNRVEYTGINLDLLQNLSDKWAITDFDLNTFIHH
                     GLISKNERVKILGRGQISSKLNVTAHAFSKKAREVIESLGGTVTLI"
     gene            3048..4400
                     /gene="secY"
                     /locus_tag="PLF20_08035"
     CDS             3048..4400
                     /gene="secY"
                     /locus_tag="PLF20_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOX80986.1"
                     /translation="MKKIIQTLRNIFQIEELRQRLLYTLGIILIYRLGAYITIPGIDP
                     NQLTAIRNQTKDGLLGLLNMFSGGAFSHASIFALGVMPYISASIVIQLLGIAVPYFQK
                     LQKEGESGRKKINQITRYLTIAVTALQAPGYIANLINQSPDAVLLVNPASNSPTTFFW
                     ISSVVILVAGTMFVMWLGERITDKGIGNGISLIIMIGIIARLPFALAGEFVSRMEEKG
                     GGLVFFVIEIAVLIGVILVTIMLVQGTRKIPVQYAKRIVGNKQYGGVRQYIPLKVNAA
                     GVMPIIFAQAIMFLPLTIAGFARSETLTGFAAAFTNINSFWYNFVFAILIIVFTYFYT
                     AITVNHNQMAEDMKKNGGFIPGVKPGKKTAEFIDNVMSRITLPGSIFLAFVAIMPAIV
                     RLFGVSSQFAQFYGGTSLLILVGVVLDTLQQIESHLLMRHYDGLMKSGRIKGRTSFAM
                     "
     gene            4415..5197
                     /gene="map"
                     /locus_tag="PLF20_08040"
     CDS             4415..5197
                     /gene="map"
                     /locus_tag="PLF20_08040"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOX80987.1"
                     /translation="MSLVKSPEEIELVRQSSLLVGKTLAEVGKHIRPGIKTIELDKIA
                     EEFIRDHGAFPGFKGYNGFPKTLCISVNSVVVHGIPGNYELKEGDIVSVDCGTILNGY
                     WGDSAYTFAVGEIEPRIQMLLQRTLESLYKGIEAAVAGNRVGDIGHAIQSYVESFGYS
                     VVRDLVGHGLGTNMHEKPEVPNFGRRGTGQKLQEGMILAIEPMVNLGSWHIIQDRDGW
                     TIRTKDGMPSAHFEHDIVVLKGKAQILSTFDFIDEVLTSRKN"
     gene            5199..5417
                     /gene="infA"
                     /locus_tag="PLF20_08045"
     CDS             5199..5417
                     /gene="infA"
                     /locus_tag="PLF20_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOX80988.1"
                     /translation="MAKQVSIEQDGTVIEALGNAMFRVELENGHRIIAHISGKMRMHY
                     IKILPGDKVKVEMTPYDLTKGRIIFRYK"
     gene            5451..5567
                     /gene="rpmJ"
                     /locus_tag="PLF20_08050"
     CDS             5451..5567
                     /gene="rpmJ"
                     /locus_tag="PLF20_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOX80989.1"
                     /translation="MKVRASVKRRTPDCKIVRRKGVVYVINKKNPKYKQRQG"
     gene            5609..5989
                     /gene="rpsM"
                     /locus_tag="PLF20_08055"
     CDS             5609..5989
                     /gene="rpsM"
                     /locus_tag="PLF20_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOX80990.1"
                     /translation="MARIAGVDLPRNKRGEIGLTYIYGIGRSTSRKILSEAGIDYDKK
                     VAEWNDEELSKVRNIITNHYKVEGALRSEVQMNIKRLMDIGCYRGIRHRLGLPVRGQA
                     TKNNARTRKGKKKTVANKKKATKG"
     gene            6032..6430
                     /gene="rpsK"
                     /locus_tag="PLF20_08060"
     CDS             6032..6430
                     /gene="rpsK"
                     /locus_tag="PLF20_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOX80991.1"
                     /translation="MAKSTKTVKSTKKRVVKVDATGRVYIHSSFNNIIISLTNNNGEV
                     ISWASAGKMGFRGSKKNTPYAAQIAAQECAKVAYDLGLRKVKAYVKGPGAGRESAIRT
                     IHSSGIEVTEIRDITPLPHNGCRPPKRRRV"
     gene            6468..>6935
                     /gene="rpsD"
                     /locus_tag="PLF20_08065"
     CDS             6468..>6935
                     /gene="rpsD"
                     /locus_tag="PLF20_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOX80992.1"
                     /translation="MARYIGPKTKIARRFRDPIYGPDRYFEKKNYPPGQHGQSRRRGK
                     QSEYGMQLAEKQKAKYTYGILERQFRNIFQKAARMRGITGEKLLQLIESRLDNVVFRL
                     GIAPTRASARQLVSHRHIMVNGKIVNIPSYLLKPGDIISIREKSKTLEVITDSL"
BASE COUNT         2240 a         1323 c         1532 g         1840 t
ORIGIN      
        1 cgaaaccgat gccgaagctt atgcattgtt aaaagcattt ggattcccat ttaaaaatga
       61 aaaataatca ggcttatggc aaaagaatca atgaaagcgc gagaagtaaa acgaaaaatg
      121 ctgaatgaaa agtatgctgc caagcgagaa gctttgaaga aggccggtga ttatgtagcc
      181 cttcagaaac ttcctcgcaa tgcttctccc gtaaggctgc acaaccgttg taagattacc
      241 ggacgcccta aaggctatat gcgcgatttt ggcctttcgc gcatcaactt tcgagaaatg
      301 gcttcggctg gtcttatccc aggtgtgaaa aaggcaagct ggtaaccatc aatttgtaat
      361 aaaaataaat agatatatga ctaccgatcc cattgcagat tatctgaccc gattgagaaa
      421 cgcaatccac gccaaccata ggattgttga aatcccttct tcaacgataa agaaaagcat
      481 gacacaaata ctcttcgaaa aaggatatat aatcaactat aagttcgaag acgataaatt
      541 cccttctacc attaaaatcg ccttgaaata ccatcctgta acaaaggctt gcgctataaa
      601 ggaactcaaa cgcataagcc gtcctggacg tcgaatttat acctctgccg ataaaatgcc
      661 tcgtgttctc aatggccttg gcatagcaat tatctctacc tcacaaggtt tgatgacaga
      721 taaggaagcc cgcaaattaa atattggcgg tgaagtgatt tgttatatta gctaattaaa
      781 accagttaaa atgtcgagaa ttggtaaaaa acctattgaa ataccttcag gagtgaaagt
      841 ttcgctcagc cccgatcatt tcattactgt acagggcaaa ctgggagaac tctgccagaa
      901 aattgacccc gccattaagg tgaatataga aggcggagag attcgactcg aaatagacaa
      961 cccggatgac aagaatctgc gagcaaaaca tggactttac cgctctttga taaataatat
     1021 ggtgattggg gtttacgaag gttttacaat tcaacaggaa attgttggag taggctataa
     1081 agttgcggtc agcgggcaac ttcttgaatt ttcgttgggg tattcgcata gcctttttct
     1141 ggaacttcct cccgaaatta aggccgaagc cgttaccgta agagggaaaa atcctatact
     1201 aatcttaaaa tcgcatgata aacaattgtt gggaatggtt gcccggaaaa tccgttcctt
     1261 cagaccaccc gaaccttaca aggggaaagg catacgtttt gtgggtgagg tagtaagacg
     1321 taaagctggt aaatcagcct catcgaaata atttttaaaa cttcgaacgc aatatgtcaa
     1381 ctaaaaatag aaaactgaaa agacgccaac ggataaaaac gagtatccgc aaacgtttaa
     1441 gaggaacacc cgaaaggcca agattaacag tatacaggag taatagccaa atttatgctc
     1501 aaattgtaga tgatacccaa ggcaagactt tgattgctac ctcgagttta caaaaagatt
     1561 tttcaaggga aaagatgacc aaaacacaac aagctcgcat agtaggagtt gaaattgccc
     1621 gtaaagctct cgaagcaggt atcacagcag ttgtttttga tcgtaatggc tttctttatc
     1681 atggtagggt caaagctctg gccgatgccg caagagaaaa tggtttaatt ttttaaattt
     1741 tgaatatggc taacgcaaat ataagaagag taaaatcgag cgaaatcgaa ttcaaagata
     1801 gactggtaag cattcaacgt gtgacaaagg ttacaaaggg tggtcgtgct ttcagttttt
     1861 ctgccattgt tgtggtagga aatgaaaatg gggttgtggg atatgggctt gggaaagcca
     1921 aggaagttac ggctgctata tctaaaggta ttgatgatgc taagaaaaat ctcattcgag
     1981 taccgataat taaaggcacc attcctcacg ctcaggaagg gaaatatagc ggagccgtag
     2041 tgtttctcaa accagccgct cccggaacgg gtgtcattgc tggtggcgcc atgcgtgccg
     2101 tgcttgagag tgtcggaata aaagacgtac tggccaaaag caagggatct tccaatcccc
     2161 atagcgtggt gaaagctacc atcaatgctc tgttgcaact gcgcgatccc tatactgttg
     2221 ctcagttgcg cggtgtggat ctcgatactg ttttcaatgg ttgaactact ataatgcttt
     2281 aaacattatg aaaagaatta gaataaaaca agttcgaagc tggattggaa aacccatcga
     2341 ccagaaacgt acactcaagg cgttgggact tggccgtttg aaccgtacgg ttgaacatac
     2401 cgctacaccg cagattctag gaatggtcag aaaagttgct catttagttt ctgttgaaga
     2461 aatttgatta attcattgat atttatctaa aactgaaaaa aatatggatt taagtaatct
     2521 gaaacctgcc aaaggatcag taaaatcgaa aagacgtttg ggaagaggcc aaggttcagg
     2581 ccgtggtgga acttccactc gcggaaacaa gggagctcaa tcgcgctcgg gtaacaaaag
     2641 aaagattggt ttcgaaggcg gtcaaatgcc actgcatcgc cgtgttccaa aaggtggatt
     2701 caaaaatttc aatcgtgtgg aatataccgg catcaatctc gaccttctac aaaacctgtc
     2761 ggataaatgg gccatcaccg attttgatct caatactttt attcaccatg gactcatcag
     2821 taagaatgaa agagtaaaaa tccttggtcg gggccagatt tcttcaaagc tgaatgttac
     2881 agcacatgct ttttccaaaa aagcacgcga agtcattgaa tcattgggtg gtacagtaac
     2941 tttgatataa ttccagatga aagccgatcg tgaaattatc aatagaaagg aacatataga
     3001 caatccgaaa gtattcactc agcctgaatt aaacaattaa attacagatg aaaaaaatta
     3061 ttcagacact tagaaacata ttccagatag aggaacttcg acaaagattg ctttataccc
     3121 tgggtatcat cttgatatat cgtttgggtg cttatattac cattccgggc atcgatccca
     3181 atcaattgac tgccattaga aaccaaacta aagatggctt gcttggtctg ttgaatatgt
     3241 tttcgggtgg agcattttcg catgcttcaa tctttgctct tggagtaatg ccatacatct
     3301 cggcttctat cgttattcag cttttgggta tcgctgttcc ttattttcag aaactgcaga
     3361 aggaaggtga aagcgggcga aaaaaaatca atcagattac ccgctatctt acgatagctg
     3421 ttacagccct tcaagcccct ggttatattg ccaacctcat caatcaaagt cctgatgcag
     3481 ttttgctcgt caatcctgca agtaattcac ccacgacttt tttctggata tcaagtgtcg
     3541 ttattttggt agcaggtacc atgtttgtga tgtggttggg cgagaggatt accgataaag
     3601 gcataggcaa tggaatttca cttatcatta tgattggtat tatagccaga ttgccttttg
     3661 cgctagccgg ggaatttgta tcgagaatgg aagaaaaagg cggcgggctg gtgttcttcg
     3721 ttatcgaaat cgccgtattg atcggtgtaa tcttagttac aattatgttg gtacagggaa
     3781 cccgtaaaat tcctgtacaa tatgccaaac gtatcgttgg caataagcaa tatggaggtg
     3841 tacgtcaata catccctctg aaagtgaatg ctgccggtgt aatgcccatt atctttgctc
     3901 aggctattat gttcctcccc ctcaccatag ctggttttgc tcgttcagaa acccttacgg
     3961 gttttgcagc tgcatttacc aatattaata gcttttggta caactttgtt ttcgcaattc
     4021 taattatagt gtttacttac ttttatacag caattaccgt aaaccataat cagatggctg
     4081 aagacatgaa gaaaaacggt ggattcattc ccggagtaaa acccggtaag aaaactgccg
     4141 aattcattga taacgtcatg tcgagaatta ctttgcccgg ttctatattt ctggctttcg
     4201 ttgctattat gcctgccatc gtcaggctat tcggagtaag ctcgcagttt gcccaatttt
     4261 atggaggaac ttctttgctt attctagtag gagttgttct ggatacatta caacaaatcg
     4321 agagccacct gctgatgagg cattatgatg gattgatgaa gtcgggaaga ataaagggac
     4381 gtacctcctt tgctatgtaa attcgagtat cattatgagt cttgtaaaat cacccgaaga
     4441 aatagagttg gtacgtcaaa gttctttgct tgttggtaag acccttgcgg aagttggcaa
     4501 acatatccgg cccgggataa aaaccatcga acttgacaag attgccgaag agtttatacg
     4561 cgaccatgga gctttcccgg gatttaaggg atacaacgga ttccccaaaa ccctatgtat
     4621 ttcggttaat tccgtcgttg tacatggaat tccgggaaat tatgaattaa aggagggcga
     4681 tatcgtatca gtcgattgtg ggactatctt gaatggatat tggggtgatt cggcttatac
     4741 ctttgctgtt ggcgaaattg aaccgaggat tcaaatgctt ctgcaacgta cgctcgaatc
     4801 actttataaa ggcattgaag ccgcagtggc aggaaaccgt gttggtgata taggccatgc
     4861 catacaatcg tacgtcgaat cttttggtta ctccgttgta agagaccttg taggccatgg
     4921 cttgggaact aacatgcacg aaaagcctga agttcctaat tttggtcgta gaggaaccgg
     4981 acagaaactc caagaaggca tgattttagc tatcgaaccc atggtaaact tgggatcatg
     5041 gcacatcatc caagatcggg atggctggac catcaggacg aaagatggga tgccatcggc
     5101 tcacttcgaa cacgatatcg tcgttttgaa aggaaaggca caaattttgt cgactttcga
     5161 ttttattgat gaagtcttaa caagtcgaaa aaattgatat ggcaaaacaa gtatctatcg
     5221 aacaagacgg tacggtgatc gaagctttgg gaaacgcaat gtttagagta gaactcgaaa
     5281 atggacatcg aatcattgct catatttcgg gaaaaatgag aatgcattat atcaaaattc
     5341 ttcccggtga taaagtaaag gttgaaatga ccccttatga tctgacaaaa ggtagaatca
     5401 tttttcgtta taaatagttg aataataatc aataaaatat ctgacccatt atgaaagtaa
     5461 gagcttcagt gaaacgcaga actcccgatt gcaaaattgt aagacgcaaa ggagtagtgt
     5521 atgtgattaa taaaaagaac cctaagtata aacagagaca gggttaagaa agaattttat
     5581 aattttggaa ttcaattaag tacaaaatat ggcaagaata gctggtgtag atttaccaag
     5641 aaacaaaaga ggcgaaattg gcctcaccta tatttatggt attggtcgct ctacttcgcg
     5701 aaagattctg agcgaggctg gaatcgacta tgataaaaaa gtggctgagt ggaacgacga
     5761 agaactttcc aaagtacgga acataattac caatcattat aaggtggaag gagctcttcg
     5821 ttcggaagtg caaatgaata taaaacggct aatggatatt ggttgttaca gaggcattcg
     5881 tcatcgcctg ggattgcccg tacgcggcca ggcaaccaaa aacaatgccc gcacacgcaa
     5941 aggcaagaaa aaaaccgttg ccaataagaa aaaagctaca aaaggatgat atcataatgg
     6001 aaattgatta taataaagta aaataaagga aatggcaaag agcactaaaa ctgtgaaatc
     6061 caccaagaaa agagtcgtaa aggtggatgc aaccggaaga gtatacattc attcgtcgtt
     6121 caacaacatt attattagcc ttaccaataa caatggtgag gttatatcgt gggcttcggc
     6181 gggaaagatg gggtttagag gttcgaaaaa aaatacaccc tatgctgctc aaatagctgc
     6241 acaggagtgt gccaaagtag cttacgattt gggattacga aaggtaaaag cttatgtaaa
     6301 aggaccgggt gcaggtcgag aatctgccat acgaaccatt cattcttcgg gcatcgaagt
     6361 taccgaaatc cgcgatatta ctccattgcc acataatggc tgccggcctc ctaaaagaag
     6421 acgcgtttga ttcttcgaat ataaaaatat gttaaaatat aatccttatg gcaagataca
     6481 ttggtccaaa gactaaaata gcaagaagat tcagggaccc gatctatggt cctgatagat
     6541 atttcgagaa gaagaattat cctcctggcc agcatggtca aagccgacgc cgtggaaaac
     6601 aaagtgaata tggtatgcaa ttagccgaaa aacaaaaggc aaagtatacc tatggaattc
     6661 tcgagcgcca gttcagaaat atatttcaaa aagcagcccg tatgagagga attaccggcg
     6721 agaaattgct tcagctcata gagtcacgtc tcgataacgt tgtattcaga ttaggcattg
     6781 cacctacgag ggcttcggct cgccaactgg tttcgcaccg tcatatcatg gtaaatggca
     6841 aaatcgtgaa tatcccttcc tatttattga aaccaggcga tataataagc ataagagaaa
     6901 aatcaaaaac tctcgaagtg attaccgaca gcctt
//
LOCUS       DAOORU010000127         6856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_13563_length_6856_cov_4.893692, whole genome shotgun sequence.
ACCESSION   DAOORU010000127 DAOORU010000000
VERSION     DAOORU010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6856
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13563_length_6856_cov_4.893692"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..706)
                     /locus_tag="PLF20_08070"
     CDS             complement(227..706)
                     /locus_tag="PLF20_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80993.1"
                     /translation="MNQSKTGIFVLPYTCKSVGYALIAVGIIWWVICQHFGITIRIPV
                     FALISSFVSVKVFSIVQTNIVDEIALILMLAGCFLAVFSKERLESEMVNNIRYRSIFL
                     ALLINQLILLFSTLFFYGSAFIVVVLANIVLVSIIYLIILRIELQKIRHSDTTIENH"
     gene            complement(746..1381)
                     /locus_tag="PLF20_08075"
     CDS             complement(746..1381)
                     /locus_tag="PLF20_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80994.1"
                     /translation="MKKLVSVFCAITFAAFLFNGCDDDSDYSIGGSPSELGNKGVIIT
                     SSSQPIAGISGFSATVTSVEDGVSVFEGHAVITNNALKQILSNVPGLNISEDNVSVSG
                     VKVKFTDKGIENKYPFYQGILVKFNAKVGDTYPVKDSDTERKVIYKSTTDDFDYAFYK
                     IKVIEVEHEINFHGLKKINYWVNHKFGLVKVQFELDNGETIAMPFYSGSLK"
     gene            1920..3290
                     /locus_tag="PLF20_08080"
     CDS             1920..3290
                     /locus_tag="PLF20_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HOX80995.1"
                     /translation="MNIWLHFIAIGGSAMHNLALALAEKGYTITGSDDVIFEPSRSRL
                     ARKGLLPEREGWFPEKLDPNIDAVILGMHAKADNPELLRAKELGLKIYSYPEYLYEQS
                     KDKMRIVIGGSHGKTTITAMVLHAFRQAGIDTDYMVGARLEGFDVMVKLSHNAHFMVI
                     EGDEYLTSPIDPRPKFHLYHPHIALLSGIAWDHFNVFPSFEIYRKQFEIFVSLIEPAG
                     ALVYCKEDESVRRIASNAPNNIQKISYSFPEFKIQDHTTLIKAIDEWFPLQIFGKHNL
                     LNLSGAMKVCQLIGIDTLTFMKAMTSFRGASNRLELIAQNELTAVYRDFAHSPSKVKA
                     TIEALVGQYPERNLVACLELHTFSSLNKDFLPQYKGSMEMAHKRIIYFNPHSLQLKNL
                     PPLFADEIKKSFDDDSLQVFNDIDALLAFLETLSWKNSNLLLMSSGNFDGLDSKRLAD
                     KILKHS"
     gene            3336..5303
                     /locus_tag="PLF20_08085"
     CDS             3336..5303
                     /locus_tag="PLF20_08085"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOX80996.1"
                     /translation="MSFVRYYHIILIIILISNCWDMKAQRSQLSTASSRAEKAYYKAT
                     ECLDHRDYKCAERELRKAVSADEGFSEAWMVLAEVLVDQNKIDEAIKIYQTILIKGRR
                     FYPEALYFLGSLELNTGLYKEASRHLSDFLNAQPISRTKEESARVKLASCEFALQAIQ
                     HPVPFEPHNLGPAVNSEYSEYFPCLTIDNQILLFTRRLPDPDIEGREQEDFFISRKQN
                     GNWSKAENIGFPINTRYNEGAPTLSPDGNFLIFTACSNQFGYGEGREGYGSCDLFISR
                     KMGQHWGRPKNIGFPVNTSKWETQPSFSSDGRTLFFIRGFYDKQNSYHQDIYQTILQD
                     DGSWSEPEKLPDCINSPGLEESVFIHPDGQTLYFSSDGWPGMGGLDIFVSRKDSSGQW
                     LTPINMGYPINTYHDENSLMVSADGKLGYFATDRPGGYGKLDLYSFEIPPELRPRIIT
                     YLKGTVFDEADGKKLAAKFELIDLATGQTIIQSESDPVDGSFLVALPSGRRYALNVSH
                     PDYLFYSDHFDLEGNHDYDHPFLKDVPMQAMKIGESVVLRNVFFATDSYELMPESRAE
                     LDRLAALLMQNPNLQIEIAGHTDSQGNDDYNMELSLKRARAVYSYLISKNIDPLRLTY
                     KGYGETKPVDTNDTPEGRANNRRTEFKIIHL"
     gene            5316..5912
                     /locus_tag="PLF20_08090"
     CDS             5316..5912
                     /locus_tag="PLF20_08090"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HOX80997.1"
                     /translation="MIEWFEAHPGFYTYAIIPFLIFFARICDQSIGTLRLILISKGYK
                     TIAPFIGFFEVIIWVLAISQIFKHLDNIVCYLAYGAGFATGNYIGILLEERISLGTAL
                     IRIIPRYDTTDLITYLSERGYGLTFWDAEGRYGNVKIILTIIKRKEIPQVVDIINRFN
                     PNAFYTIEEIKAVNKGILKDEKATDIFRNLIFGPRKAK"
     gene            6340..6498
                     /locus_tag="PLF20_08095"
     CDS             6340..6498
                     /locus_tag="PLF20_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX80998.1"
                     /translation="MRKEKMESGFNEIVDEEIKVIQKDSDTDYGYIKMTYDLSCLKIL
                     INIKKTYS"
BASE COUNT         2183 a         1364 c         1287 g         2022 t
ORIGIN      
        1 atacacagat ggataaaaat ggtggatggt atatggtgat tggtaagtta aaagttcata
       61 aagtttaaaa cggaagaagg gagaacggag gttggtgatt agtgattagt ggatgttgat
      121 tggtgaatgg cgattggagc ttgttcgttg tcagttatta gttgttagtt gtcaattgtt
      181 ggttgtattg caaaaaatgg tcaaaataag attaacagca aagatttcaa tgattctcga
      241 ttgtggtgtc tgaatgccgt attttctgta attctattct caatattatc agatagataa
      301 tggacaccaa gacaatattg gccaatacaa cgactataaa cgcacttcca taaaagaaga
      361 gcgtcgagaa aagcagtatg agttgattga tcaataaagc cagaaagatt gaacggtatc
      421 tgatattgtt caccatttca ctttctaacc tttccttgct gaaaacggca aggaaacagc
      481 ctgcaagcat caaaatcaat gcgatttcgt ccacaatatt ggtctgaaca atcgaaaaaa
      541 ccttcaccga tacaaaagat gaaatcagag caaaaacagg gattcttatc gttataccaa
      601 aatgttgaca aatcacccac caaataattc cgactgcaat tagggcatat ccaacagact
      661 tacaggtgta tggcaaaaca aaaatccctg tttttgactg gttcatgtta ggttaatcca
      721 aacaaatttt aagcaaactg ataaatcatt tcaacgaccc agaataaaaa ggcatagcga
      781 tggtttcacc attatctaat tcaaactgca cctttacgag tccgaattta tgatttaccc
      841 aataattaat tttcttcaag ccatggaaat taatttcgtg ttcaacctca attaccttga
      901 ttttgtaaaa agcataatcg aagtcatcgg tagtggattt ataaatcacc ttgcgttctg
      961 tatccgaatc tttgacggga taggtatcac ctacctttgc attgaatttt acgagtatcc
     1021 cttgataaaa aggatacttg ttttctatcc ctttatcggt aaatttaact tttactccac
     1081 tcacactcac attgtcttca ctgatattaa ggccaggaac atttgacaag atttgtttaa
     1141 gggcattgtt ggtaattaca gcatgaccct cgaatactga tactccatct tcgactgaag
     1201 taacggtggc cgaaaatcca gaaatgccag caatcggctg ggatgaagat gttattatta
     1261 cacctttatt ccctagttca gaaggtgatc ctccgatact ataatcggaa tcatcatcgc
     1321 aaccattgaa taaaaatgca gcaaaagtaa ttgcacaaaa aacagaaact aactttttca
     1381 tagtcataag gatttaaggg ttaatggtaa gttttgattg atattatgaa tttagtcata
     1441 catgcttgaa ttattctatc ttccattgaa aaaatactga actggttcat acaacatcgt
     1501 ataaaactcc tttttggatt ttcattttct ttgaagaaat gagctctctc cgcactccgt
     1561 attctgctct tctgtaacct cattccccaa ttcattattt tcttgcgaag ggaaaaatta
     1621 atatttgatt ttggttatgc aaagatacat ttctctccat ggcttgtata agttttatta
     1681 aaatttttat aaaagaaatt atcaaaccgc catcttgttg gtacttatat tttactttga
     1741 accaagtcca ttcatttagt gactttaccg cggcatttag taatttcata attaaacata
     1801 agatttcgta tcaaaattat tgaattcctt catttcatca agtatagaat gataaacagg
     1861 agaaataagc gagtttaatt tctaatttcg tagttttaat ttaccgaaag aaatcttgaa
     1921 tgaacatttg gctacatttc attgcaattg gaggcagtgc catgcacaat ctggctttgg
     1981 ctcttgctga aaaaggatac acaataacag gcagcgatga tgtaattttc gaaccttcac
     2041 gttcacgtct tgcccgaaaa ggtttactcc ctgaaagaga aggatggttt ccagagaaac
     2101 tcgatcccaa tattgatgca gttatccttg gaatgcatgc caaggccgac aatccagaat
     2161 tgctcagggc aaaagaactt ggattgaaaa tctattccta tcccgaatat ctttatgagc
     2221 aatcgaaaga taaaatgaga atagtgatag ggggaagcca tggcaaaacc accattactg
     2281 ccatggtctt gcatgctttc cgacaagcgg gcatagatac cgattatatg gttggagctc
     2341 gcctcgaagg tttcgatgta atggtaaaac taagccataa tgctcatttt atggtgattg
     2401 aaggagatga atatcttact tcacctattg acccacgccc caaatttcat ctctatcatc
     2461 ctcatattgc attgctgagt ggaatagcat gggatcattt caatgtgttc ccttcattcg
     2521 aaatttatag aaaacaattc gaaatcttcg tttctttaat cgaacctgct ggcgctcttg
     2581 tgtattgcaa agaagatgaa tcggttagac gaattgcctc gaatgctccc aataacatcc
     2641 aaaaaatttc ctattctttc ccagaattta agattcaaga ccatacaacc ttgataaaag
     2701 ccatcgacga atggttcccc ttgcaaatat tcgggaaaca taatcttttg aatttgagtg
     2761 gggctatgaa ggtttgccaa ttaataggaa tcgatactct cactttcatg aaagccatga
     2821 caagttttcg tggtgcatca aaccgtctgg aactgatagc ccaaaacgaa cttacagcag
     2881 tttatcgcga ctttgcacat tcaccttcca aagttaaagc cacaatagaa gcccttgttg
     2941 gccagtaccc cgaacgaaat ctcgtggctt gtcttgaatt gcataccttt agcagcctca
     3001 acaaagattt tcttcctcag tataagggaa gcatggaaat ggctcataaa agaataatct
     3061 attttaatcc tcattccttg cagttgaaaa acctcccacc cctgtttgcc gacgaaataa
     3121 aaaaatcatt cgatgacgat tcattacagg tcttcaatga cattgacgca ttattagcct
     3181 ttctcgaaac cctgtcatgg aagaattcga atttgctact aatgagctcg ggcaacttcg
     3241 atgggctcga ttctaaaagg cttgccgata agattctgaa acattcgtaa taaagttgta
     3301 ttttcgtacc ttatccaaat actaaatttt attgaatgtc ttttgttcga tattatcata
     3361 ttattcttat catcatcctg atttcgaact gttgggatat gaaggcacaa cgaagtcagc
     3421 tctcgactgc ttcatccaga gccgaaaaag cttattataa agccacggaa tgtttggatc
     3481 atcgcgatta caaatgtgcc gaaagggaat tacgaaaagc agtctctgca gatgaaggtt
     3541 ttagcgaagc atggatggtt ttggccgaag ttttggtcga tcaaaacaaa attgacgaag
     3601 ctattaaaat ttatcaaacg attttaataa aagggcggcg tttttaccca gaagcactct
     3661 attttttagg ttcactcgag ttaaacactg gattgtacaa agaagcttct cgacatttaa
     3721 gcgatttttt gaatgctcaa cctatttcaa gaacaaaaga agaatcggca cgtgtaaagc
     3781 tggcttcctg cgaatttgct cttcaagcca tacagcatcc agttcctttc gaacctcata
     3841 atttgggacc tgctgtcaat agcgaatatt ccgagtattt cccctgcttg acaatcgaca
     3901 accagatctt gctttttacg cgtcgccttc ctgatcctga tatagaaggc cgcgaacagg
     3961 aagatttctt catcagtaga aaacagaatg gcaattggag taaagccgaa aatattggtt
     4021 tccccatcaa tacacggtac aatgaaggtg cccctaccct ctctcccgat ggtaattttc
     4081 tgatattcac tgcatgcagc aatcaattcg gatatggtga aggtagagaa ggttatggaa
     4141 gctgtgatct atttatttcc cgcaaaatgg gtcaacattg gggtaggcct aaaaacattg
     4201 gcttcccggt aaatacttct aaatgggaaa cccagccttc tttttcgagc gatggaagaa
     4261 ccctcttctt tattagaggc ttttacgata agcaaaattc ttatcatcaa gatatctatc
     4321 aaaccattct acaagatgat ggttcatgga gcgagcccga aaagttaccc gattgcatca
     4381 atagcccggg attagaggaa agcgttttca tccatcccga cgggcaaaca ctctattttt
     4441 cgtcggatgg ctggccagga atgggaggat tggatatttt cgtcagcaga aaagacagca
     4501 gcggacaatg gttgacaccc ataaatatgg gataccccat caatacctac catgatgaaa
     4561 atagcctgat ggtttctgcc gatgggaaat tgggctattt tgccacagat cgtcccggcg
     4621 gctatggcaa actcgattta tattctttcg aaattcctcc cgagcttcgc cctcgaatca
     4681 ttacctattt aaaaggcact gtattcgacg aagccgatgg taaaaaactc gccgccaaat
     4741 tcgaactcat tgatctggct acaggacaaa ccattattca aagtgaaagc gacccagtag
     4801 atggtagttt tttggtggct ttgccctcag gcagacgtta tgccctcaat gtatctcatc
     4861 ccgactattt attttattcc gaccacttcg acctcgaagg caaccacgat tatgaccatc
     4921 ctttcttgaa agatgttcct atgcaggcta tgaaaattgg cgaatcggtc gtacttcgta
     4981 atgtcttttt tgctacggat agttacgaac ttatgcccga atcacgagcc gaactcgacc
     5041 gcctggccgc tctcctgatg caaaacccta acctgcaaat cgaaatcgcc ggacataccg
     5101 atagccaagg caacgacgac tataatatgg aactttcgct gaaacgagct cgggcagttt
     5161 acagctacct aatcagtaaa aacatcgatc cactgcgctt gacctataag ggatatggag
     5221 aaactaaacc agtggatacc aacgatacac ccgaaggtag ggccaataac cgccgaaccg
     5281 aattcaaaat tattcacctt taaaattgat taacgatgat agaatggttt gaagctcatc
     5341 ctggatttta tacttatgct attatccctt tccttatttt ctttgccaga atttgcgatc
     5401 aaagtattgg aacccttcgt ttgatcctga tttcgaaagg gtataaaacc attgcacctt
     5461 ttataggatt tttcgaagtc attatctggg tgctggccat ttcacaaatc tttaaacacc
     5521 tcgataatat agtatgctac ttggcttatg gagcaggatt tgccacaggt aactatatcg
     5581 gcatattgct cgaagaacgt atctcgcttg gtacagcact tatcagaatt attcccagat
     5641 atgatactac tgacctaatc acatacctga gtgaacgggg ttatggcctt actttctggg
     5701 atgccgaagg ccgatatgga aacgtaaaga ttatcctcac catcatcaag cggaaagaaa
     5761 tacctcaggt ggtggatatt atcaataggt ttaatccgaa tgcattttat acaattgaag
     5821 aaataaaagc cgtcaataaa ggcattctaa aggatgaaaa ggcaactgac atttttcgca
     5881 atctaatttt tgggccgcgt aaagcaaaat gatgacccta tgcgagatac acgttaaggg
     5941 ctatgaggta taggggattt tgatgaatgg ggaaaataga aaccagagtc agaagagaaa
     6001 gaaaaaatta atatttggtg attgtgaaat aaggaatagg caggaagtat cgccccatct
     6061 tccccttcac atggacactg catcacttat catttttaaa tttttactca aagataacca
     6121 tattttatag ccccacacct tcatatatcc gattctttgg aatttttatt atcactaatc
     6181 ttccttattt ggatttgctc cctgaatatt ggataaaagc atggatagaa atctgaactt
     6241 taatacccaa aaaaattcac aataaagaag aagtctaaaa tcataaaaaa acagcctccc
     6301 tgagcagggt tgatcctgcc aattgaaacc aaacttgctg tgagaaaaga aaaaatggaa
     6361 agtggattca atgaaatagt ggatgaagaa ataaaagtca tccagaaaga ttctgatact
     6421 gattatggat acatcaagat gacctatgat ttatcatgct tgaagattct tattaacata
     6481 aaaaaaactt atagctaatg gtcgaaaacc aattgcttca taaccaggtg caagaataat
     6541 tatcgggggg aaaagccgat agaaacaacg tatgaagagc aacataaaaa aaggcatcat
     6601 tagctcctca tcccctatca tcaatcatcg ggaaatatga gtttgagagg acctctttgt
     6661 ccttatttga aattcctcgt tggcatagag gatgataact tagaaaaaga aatgtccgtc
     6721 gctgaaacat ttccataacc atcatcggca taataaacac tgctggttgt tcattgtcga
     6781 cgtaaaattc actgattttt tctatttttc tcctataaat cctacccaag ttataccgcc
     6841 tttggggaag cgagtt
//
LOCUS       DAOORU010000128         6819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_13638_length_6819_cov_8.078208, whole genome shotgun sequence.
ACCESSION   DAOORU010000128 DAOORU010000000
VERSION     DAOORU010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13638_length_6819_cov_8.078208"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            768..3608
                     /locus_tag="PLF20_08100"
     CDS             768..3608
                     /locus_tag="PLF20_08100"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOX80999.1"
                     /translation="MKRTLFLTGFLLMGFWLNAQFQYQPKASVPTDPSVKIGQLPNRL
                     TYYIQKNGQPEKRAEFYLIVNAGAILEDVDQNGLAHFCEHMAFNGSKHFQKHQIINYL
                     QSIGMKFGPEINAFTSHDVTAYMLQNVPLNDPAQMDTALLILYDWASQVSFEDAEIDA
                     ERGVIHEEWRTGRNAMERMNKQAMKKLFYNSKYAVHDVIGDIQIIDIFPYETVRRFYH
                     DWYRPDLQAVIAVGDFDPQIVEQKIVNLFGTLPKPENARERVVEKVPDHEETLVAIET
                     DKEAQFTMVEVVYKHEPIEERNQEYLHQQLVTQLFNQMMNARLSELQRQADPPFIYAY
                     DVYTNLVRSKDAYLAIAISKTGEAMRAIEALLTENERVLRYGFTEGELERAKNEILKQ
                     RENAFNERNKRKSEQLVWQYMNHFLENEPVPGPEFEYIFAKNLLPSITLEEVNALPSK
                     WITDSNRVIVVQGPQKEGLIYPTEQEVLDLLARIEKEEITPYVDKVSDKPLISELPKP
                     GKVIESSEDKATGVITWKLSNGAKVVIKPTDFKEDEILFAAISLGGSSLYPIEEYLSA
                     QFAAAIIGESGLGDFDATELQKALSGKLVDLSPYIDDLKEGFNGRSSKSDLETLFQLL
                     HLYFGKARADENIYGSHMNRLKAFLENSALDPENTFRDTIQVTMASYSPYRKPLTVER
                     LSETNLSLMKQIFDQRFADADGFTYYFVGSFRPDELKPLIEKYLASLPSVKKNEQWRD
                     LKIQPPKGTIKKTVVRDMEDPKATVFISLTGKFDYDSMKRLAMRAINDILSFRFVETI
                     REEEGATYGTSVRTRLSKYPQPQYQLSIQFDCDPENAERMTHIVYQEIEKLRTQGPTE
                     EQMRNFKENQLKTWAENIKENNFWMNKLTTSDFDGESYENILKFPEMLKILTPEMVKK
                     VAQQCYSGENVVQIILMPSDLSKSVKNPNIKP"
     gene            3958..5307
                     /locus_tag="PLF20_08105"
     CDS             3958..5307
                     /locus_tag="PLF20_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004293990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOX81000.1"
                     /translation="MDQKGSPLELGTKRIWPLLLQYSIPSVIAMTASSLYNITASIFI
                     GHGVGALALSGLAISFPLMNLAAAFGSLVGVGASTLMSLRLGQRDYASANKILGNVFV
                     LNILLGLVYTFIVLLFLDPILYFFGASKATISYANDYMLVITIGNVVTHLYFGLNAML
                     RATGNPTKSMMATISTVIINIILTPLFIFGLKWGIKGAAISTVVSQTLLMIWQIRFFS
                     NKENFIHLQKNSFRLERKIVVDSLSIGMAPFLMNAAASIIVIVVNQSLIKYGSDLAVG
                     AYGIINRGASLFVMIAVGLNQGMQPIAGYNFGAKQIDRVNKVLKLTIMLATAIMTVGF
                     IVSELFPHAVATIFTKDEQMIQIVVEGLRIVFIFFPLAGFQMVTSNFFQSIGMPGKAI
                     FMSLTRQVLFLLPFLLILPPHFGLKGVWYSMPAADLLSSLVAAYMLVTQYARSKINS"
     gene            complement(5565..5714)
                     /locus_tag="PLF20_08110"
     CDS             complement(5565..5714)
                     /locus_tag="PLF20_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81001.1"
                     /translation="MKKHHNAIIVAIARQLLTIIWTLLSKEEKFDNEYHLKKNIPSIT
                     SIKVE"
     gene            complement(5720..6034)
                     /locus_tag="PLF20_08115"
     CDS             complement(5720..6034)
                     /locus_tag="PLF20_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HOX81002.1"
                     /translation="MDSMIQKCNELYPKAFSILLGICGIDKLSAAIIISEIGDNMKLF
                     ATAAALVSWAGLRPRNDESAGKIQSHKITHGNKYLRKTLIECAWGASRKRDSVFYCRF
                     DT"
     gene            complement(6080..6598)
                     /locus_tag="PLF20_08120"
     CDS             complement(6080..6598)
                     /locus_tag="PLF20_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOX81003.1"
                     /translation="MKSHFVSEVCMESTGIYWIPVWRLLENNFQLRLVNAQFFKQLPG
                     RKSDVKDAEWIATVLLKEWARDNFVPDGNIQSIRQYGRRINEINKDLVRCQQHIDMIL
                     QRCNIRLSNYVSNMDSKSYRKMVDALIKAETSSYELVKLVHGRTIHRYSKSIIQDALE
                     GDVKQSDRDLLL"
BASE COUNT         2066 a         1301 c         1383 g         2069 t
ORIGIN      
        1 gactcatcga aaggaaccca aacgatttgc cctactaagg gagacaccgc tccccaaagc
       61 aaacaaaaaa ttaaaccaat tacattttct tcatataaat aattgaattt aagctaaata
      121 cattggtgga atacattcat ctccatcaac atcaaatata atactaataa gtaaaatttc
      181 aaaaccgcag ttaaaatatt ttttcttgga ggagaactca ttacaaggag caaacgaaaa
      241 ttctgcctat tcagagaata atacacagca gatagataag ctggcgatta gggaaaaatt
      301 ttttaagatg aaattcgaat aactgtaaat caggtttaat gattgttcaa acccctagat
      361 ccggaaaaga tatgcttctt cttgaatact tatatggatt accatttagc atcattatac
      421 tgaattatat ttcatacgga tgccgctcaa acttcattct ttaaattttt aagaatattt
      481 tttcatagaa tggattaaca acaatggatt agacttgcaa tttagatttc aattaatatc
      541 tcaggttgtt aaatcatttt atagagagag caataaaaaa agcactgcga tgtgcttcat
      601 tccattgttt tattgctgat ttaaagtctt tcgttatcaa gaaaggaaag gaataggtta
      661 ttttggtagt gtatagttta attgtttata tatttgtcct caaaatgcaa tgctaatagg
      721 gagatatttt aatagatgac ataccaatta gttaaatata aaaatttatg aaacgcactt
      781 tatttctcac aggcttctta ttgatgggct tttggcttaa tgcccagttt cagtatcaac
      841 ccaaggcatc agtgcctacc gatccttcgg taaaaatcgg gcaacttccc aataggctga
      901 cttattatat tcaaaagaat ggccagcccg aaaagagagc cgaattttat ctgattgtga
      961 atgctggagc cattctcgaa gatgtcgatc agaacggatt ggctcatttt tgcgagcaca
     1021 tggccttcaa tggcagcaaa cattttcaga agcatcagat tataaattat ttacaatcca
     1081 taggcatgaa attcgggccc gagatcaatg cctttacaag ccacgatgtt actgcctata
     1141 tgcttcagaa tgttcccctc aacgaccctg ctcagatgga tacagccttg ctgattttat
     1201 acgattgggc ttcgcaggtt agcttcgagg atgcagagat tgatgccgaa cgaggagtaa
     1261 ttcatgagga atggcgtacg ggccgcaatg ccatggaacg catgaataag caagccatga
     1321 aaaaactctt ctacaactcg aaatatgcgg ttcacgacgt gattggtgat atccaaatca
     1381 ttgacatttt cccttatgaa accgtcagga gattttatca cgactggtat cgtcccgatc
     1441 ttcaggccgt aattgcggtg ggagatttcg atcctcaaat cgttgagcag aaaattgtaa
     1501 atctgtttgg cactctacct aaaccagaaa atgccagaga aagagtggtg gaaaaggttc
     1561 ccgatcatga ggaaaccttg gtagccatcg aaaccgataa ggaagcacaa ttcaccatgg
     1621 ttgaagtggt ttataaacat gagcctattg aggaacgcaa tcaagagtat ttacaccagc
     1681 aattggttac acagttattc aatcaaatga tgaatgcccg gctgagcgaa ttgcagcgac
     1741 aggccgatcc accctttatc tatgcttatg atgtttatac caatttggtt cgttcgaaag
     1801 atgcctattt ggccattgcc atcagcaaaa cgggagaagc catgagagca atagaggcct
     1861 tgcttaccga aaatgaacgt gttttacgtt atggctttac cgaaggagaa ctggaaagag
     1921 caaagaatga aattctcaaa caacgcgaaa atgccttcaa cgagcgcaat aaacgcaaat
     1981 ctgaacaact ggtttggcaa tatatgaatc atttccttga aaatgaaccc gtacctggcc
     2041 ctgaattcga atatattttt gccaaaaact tattgccaag tattaccctc gaagaggtta
     2101 atgctttacc ttcgaaatgg atcacggaca gcaatcgggt cattgtggtt cagggacctc
     2161 agaaagaggg cctcatttat cctaccgaac aggaagttct cgatttacta gcaaggatcg
     2221 agaaagaaga aataactcct tatgtagata aagtttccga taagcctctt atttcggaat
     2281 tgcctaaacc tggtaaagtg attgaatctt ccgaagataa ggctacaggt gtgataacat
     2341 ggaaattgtc caatggagct aaagtcgtta taaaacctac cgattttaag gaagacgaaa
     2401 ttttatttgc cgcaatcagt ttgggaggct catctcttta tcctatcgaa gaatatcttt
     2461 cggctcagtt tgctgctgct atcattggtg aaagtggtct cggcgatttt gacgctaccg
     2521 aactccagaa agcgctaagt gggaaattag ttgacctatc accttatatc gatgatttga
     2581 aggaaggatt taacggtcgt tcttcgaaaa gcgaccttga aaccttattc cagttgttgc
     2641 atctttattt tgggaaagca cgtgctgatg agaatattta tggttcacac atgaatcgtt
     2701 tgaaagcatt tctcgaaaac agtgctctcg atcccgaaaa taccttcaga gatactatac
     2761 aggtaactat ggcttcatat agtccttatc gtaaacccct taccgtcgaa cgactatcgg
     2821 aaaccaacct cagcctgatg aaacaaatct tcgaccagcg ttttgccgat gccgatggat
     2881 ttacctatta cttcgtaggg agctttcggc ccgatgaatt gaaacctctg attgagaaat
     2941 acctggcttc tttaccttcc gtgaagaaaa acgagcagtg gagggacctg aaaattcaac
     3001 ctcccaaagg cacaatcaaa aaaacagtgg tgcgtgatat ggaagaccct aaggctacgg
     3061 tatttatttc gcttaccgga aagttcgatt atgattccat gaaacgcctg gccatgagag
     3121 ctataaatga tatattgagt tttagatttg tagagactat acgcgaagaa gaaggagcta
     3181 cctatggcac aagtgttaga actcgattgt cgaaatatcc ccagccacaa taccagctca
     3241 gtattcaatt cgactgtgat cccgagaacg ccgaacgcat gacccacatc gtctatcagg
     3301 aaatcgagaa attgcgtact cagggtccaa cggaggaaca aatgcgtaat tttaaggaaa
     3361 atcagctaaa gacatgggca gaaaatatca aggaaaataa tttctggatg aataaactta
     3421 ctacaagtga ttttgatgga gaatcgtatg aaaatatcct gaaattccct gaaatgttga
     3481 agatcttaac tccggaaatg gtgaagaaag ttgctcagca gtgctattcg ggcgaaaatg
     3541 tagtgcaaat tattctcatg ccttccgact tgagtaaatc ggtgaagaat ccgaatataa
     3601 aaccttaaaa aggtaagggt tttgaagggt ggagaaggaa gaatagagaa tggattataa
     3661 agaaatatgg aatagggttt gggaaacaac agataaaaaa acgtttcgta ttgcgaagag
     3721 gcaaggagac gtagagatag agagagtgga agacatagat gctttgcagg tctgacgact
     3781 ttacagagaa ttatatcctt cgtagtgcgg cggagggggg gaagtgtagt acattggaag
     3841 gttaatctta aaagagtggt aattttgccg aatttctgat ttccttcaaa gagacgataa
     3901 gggaagaggt cagataaatg tatttggtca ttttagggca tttttgatat taaactaatg
     3961 gatcaaaaag gttctccact ggaattaggc acgaagagga tatggccttt gcttctgcag
     4021 tattccattc catcggtgat agccatgacg gcttcttcgc tttacaacat cacggccagc
     4081 atttttatag gacatggggt tggtgctttg gctctttcgg gtttagccat cagttttcca
     4141 ttgatgaatt tggccgctgc atttggatca cttgtaggtg ttggtgcttc taccttgatg
     4201 tcattgcgcc ttggacaaag agattatgcg tcggccaata aaatcctggg gaatgttttc
     4261 gttctgaata tcctattagg tttggtttac accttcatcg ttctcttatt tctcgatccc
     4321 atactctatt tttttggagc cagcaaagcc actatctctt atgcaaatga ttatatgctg
     4381 gttattacca tcggtaatgt agttactcat ctttattttg gattgaacgc catgctaagg
     4441 gctactggaa atcctacgaa atcgatgatg gcaactattt ctacggtgat aatcaacatc
     4501 attctgacgc ctttatttat ttttggtctt aaatggggaa tcaaaggagc tgccatttcc
     4561 accgttgtct cgcaaactct tttgatgata tggcaaattc gattttttag caataaagaa
     4621 aattttatcc atctgcaaaa aaacagtttc agattggaac gcaaaatcgt tgtagattct
     4681 ctaagcattg gcatggctcc ctttcttatg aatgccgccg caagtatcat tgtcatcgtg
     4741 gtcaatcaaa gcctgatcaa atatggaagc gacctggcag ttggagctta tggaattatc
     4801 aacagaggag cttctctttt cgtaatgatt gccgtgggat taaaccaggg aatgcaaccc
     4861 atagccggct ataactttgg tgccaaacaa atagaccggg tgaataaggt actgaagctg
     4921 accatcatgc tggctacagc tatcatgact gtcggtttta ttgtgagtga actcttccct
     4981 catgccgtag caaccatttt tacaaaagat gaacaaatga tacaaattgt tgtagaaggt
     5041 ttaagaattg tctttatatt tttccccctt gcaggtttcc aaatggttac ttctaatttt
     5101 tttcagagca ttggcatgcc gggcaaagcc atttttatgt cattgaccag acaggtgctt
     5161 tttcttttac cattcctatt aatacttccg cctcattttg gattgaaagg agtttggtac
     5221 agcatgcctg ccgctgacct gctttcgagt ttggtagcag cttatatgct tgttactcaa
     5281 tatgccagat caaagattaa ttcttaaatt tcctctataa atctcataat tcttttttca
     5341 aaagtggctt ataatgagca attataaata tattatgaat attcaaagca aagttcaatc
     5401 tggcggaaga gataaggctt tcgcattaaa aatattaatt tcctcttcga actcaaagtt
     5461 tcttcacggg gttcaattcg gagagtttgc tatagtcaat ttgcgaaaac gggatattga
     5521 tccaccacaa atttaaagac tttacaaacc atccgacaca ttccttattc tacctttatt
     5581 gaggtaatag aaggaatgtt tttctttaaa tgatattcat tatcaaattt ttcttctttt
     5641 gaaagcaatg tccagatgat tgtgagcaat tgtcttgcta tagctacaat aatggcatta
     5701 tgatgtttct tcatttcttt caggtgtcaa aacggcagta aaagacagag tctctctttc
     5761 tggaggctcc ccaagcacat tctataagag ttttacgaag gtatttgttt ccatgagtga
     5821 ttttgtggct ttgaatttta cctgcgcttt catcgttgcg tggacgcagt cctgcccatg
     5881 acacaagggc tgctgctgtg gcaaacagtt tcatattgtc gcctatttct gatatgatga
     5941 tagctgctga aagtttgtcg atgccgcaaa ttcccaataa tatggaaaat gctttgggat
     6001 aaagttcgtt gcacttctga atcattgaat ccatgcattg ctgcttttga agttgataca
     6061 aatcgaacat ttgtgtgtat tacaggagca aatccctgtc gctttgttta acatcgccct
     6121 cgagagcatc ctgtattatg gattttgagt aacgatggat ggtgcgccca tgaacgagtt
     6181 taaccaactc ataggatgat gtctcagctt tgataagtgc atccaccatt ttgcgataac
     6241 ttttactgtc catgttggat acataattgc tcaagcgaat gttgcaacgt tgtaaaatca
     6301 tatcgatgtg ttgctgacaa cgaactaaat ctttgtttat ctcgttgatg cgtcttccgt
     6361 actgacgaat gctttgaata ttgccatcag gaacaaagtt gtcgcgagcc cactctttga
     6421 gtaaaaccgt tgctatccat tcagcatcct ttacatcgct ctttcgtccg ggcaattgtt
     6481 tgaagaactg ggcattgacc aaacgtaatt gaaaattgtt ttcaagcaaa cgccatacag
     6541 gtatccagta aatacccgta ctttccatgc acacttcact cacaaaatgt gatttcatga
     6601 ctgaacccat ccgttttatc tcacgggtga tcataccaaa tttttcttct gttttttgtc
     6661 ttgctcatcc cagatacaca taaatatact atctttatga acgtcaaggc cactgactgt
     6721 tctcatatgg caatagtttt taattaaaca attgtacctt ttgagtacgt tgattactta
     6781 tagaacaatc atggccttga tttttgttgt gcggcgtat
//
LOCUS       DAOORU010000129         6767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_13785_length_6767_cov_5.293057, whole genome shotgun sequence.
ACCESSION   DAOORU010000129 DAOORU010000000
VERSION     DAOORU010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6767
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13785_length_6767_cov_5.293057"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            133..1866
                     /locus_tag="PLF20_08125"
     CDS             133..1866
                     /locus_tag="PLF20_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOX81004.1"
                     /translation="MHRKSSSFFRRKSVELIIEHAEDKVEGQTSLRRNLGVADLTALG
                     IAAIIGAGIFSTIGTAAAYGGPAVSLLFIFTAIACGFSALCYAEFSSAIPISGSAYTY
                     AYASFGELIAWIIGWDLIMEYAIGNIAVAISWSGYFTGLMEGLGIPIPQFLCIDYLTA
                     SRGFHEIIESGISVEQASRQLQEAFQAWTTAPQIAGIRLIADIPAFGIVVLISWLVYV
                     GIHETKVAGNFMVLLKLITLLVVIGVGAFYVNPDNWSPFAPNGISGVLKGTAGVFFAY
                     IGFDAISTTAEEARNPRQDLPRAMIYALLITTILYILISLILTGMVPYSHLEVSDPLA
                     YVFSFHHINWLSGIISLSAIIAMAGVLLVFQVGQPRIWMSMSRDGLLPKIFSKLHPRH
                     RTPAFATIVAGILVAVPSLFLNLTEVTDLTSIGTLFAFVLVSGGILAVNPHGKKQKKD
                     DINRKFVVPYLNSRIWLLPIWTIMIGSLFYFQPEGFIKSDGLTSLIGNIPILLFAICS
                     IIISIFAILKEWSLIPVLGLLTNLYLMSQLGITNWMRFLIWLVIGLIIYFTYGYRKSR
                     LKGWLKQIKTD"
     gene            1868..2656
                     /locus_tag="PLF20_08130"
     CDS             1868..2656
                     /locus_tag="PLF20_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOX81005.1"
                     /translation="MLTWQAIALFLGGIFAGFVNTLAGGGSIVSLSLMLLFGLDANVA
                     NGTNRLAIIFQSLTSSETFRVNKILDFKRGKAAVWSALIGSLAGAWVAVDLNKEIIEK
                     IITIVMMAMLLFIFYNPSRWLKGDPERNAKPANLFEIFLFLVIGFYGGFVQVGIGYLL
                     LAGLVLQAGYDLVKANALKVFITFVYSPFVFAIFVWHHQVSWIHGIPLAIGASIGAFI
                     ASKLAIKKGGGFIRWTIVVMILITALQTFNVIDLTTLFSFLKPK"
     gene            complement(2677..4029)
                     /locus_tag="PLF20_08135"
     CDS             complement(2677..4029)
                     /locus_tag="PLF20_08135"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX81006.1"
                     /translation="MKKNKRLYWIIGAGLVVILLVILAVNKKNHNNYQRVATEKVQKR
                     NIIEAISATGKIQPVKDIKISPYISGEVIEIFVKEGDRVKVGDILVRIDPEIYKSAYE
                     QAEAQLNSQRANLVNAKAQLAQLEARFQNTETVYLRNKALWEKKVISDADFETAKTNY
                     EVAKADLDAIQATIKAAEYSISSANAALKKSREDLTKTAVYAPADGRVSKLSVEKGER
                     VMGASQFSAGTEIMRIANLDEMQAEVDVNENDIIKVKLGDTALIEVDAYLNRKFKGIV
                     TEVATSATTTGVSIDQVTNFQVKIQLLKSSYLDLIPPDQPDYSPFRPGMSTTAEIQTQ
                     RRFDVLSVPIQAVTTRSDTTGRITRKASKPEEESQTEQSENKLPEIIKEYVFVYDNGI
                     ARLREVKSGIQDNNYIEIISGLKEGEDVIIAPFSAISRKLKNNEMVEKVDREKLYETK
                     "
     gene            complement(4058..5509)
                     /locus_tag="PLF20_08140"
     CDS             complement(4058..5509)
                     /locus_tag="PLF20_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX81007.1"
                     /translation="MMNSFKILKQVILLMLLFFLPFLGYGQEIHEWTLEECITYARNH
                     NINVKKQMLNVQLQKINTTESKLTMLPTLGGNATHVYNWGQTIDPFTNQFATERVRSN
                     SFYVSTQMVLFSGFQKLNTLRKSQLDLNISELEVEKMMNDISLNVATFYLQALYNAEL
                     LNISESQLKVVKLQRDRIKILVESGSLAQGELLNMEAQVASAELQLVEARNNYELALL
                     QLSQLIDLEDSRGFSVVAPELAAPSVNISLSPVDQVVNYALQHQPEIISSKYRIESAR
                     KEFDINRGSYGPTLALNGSWGTGYSGVSLEKDPNVPPTLIAYPIGITQLTGDTVIGIS
                     PEYSYRVKPFGDQLKANENQSLGLYLNIPIFNGYRSRNNVQRTKIAVANAELDLQLSQ
                     NNLRKLIVQAYADASAALKKYVAAETKLHAQQESFNYASEKYAVGLLNAVEYNQNKED
                     LNKAQSELIQAKYDFIFKVKILDFYEGKPLVLN"
     gene            5805..>6767
                     /locus_tag="PLF20_08145"
     CDS             5805..>6767
                     /locus_tag="PLF20_08145"
                     /inference="COORDINATES: protein motif:HMM:NF037667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81008.1"
                     /translation="MMRKIRISMILLLTGILSSVQAQQLKSDTFDVVHYDLHLDIMNF
                     QAKQLNGFAILTLTPKMNQLSYISLDLLSLEVDSVRVEGQPILSWYQDDTLLRIPLLS
                     PVSIGDTFQVQIRYHGTPVVEPSGWGGFHFSSWIAYNLGVAFQANPHNYGRAWFPCID
                     DFIDRATYDYYITTEAGKTAVCGGLLIDSIVHPTNNITWHWKMNQPIPAYLASVAVAS
                     YTKIADIYTGIQADIPILLYFRPSDTAAVNNLFVNLKDILSVYENHWGPYSFDRVGYV
                     GTIEGAMEHAANIALPVSTLSSGYEWLYAHELSHMWFGDKITCSS"
BASE COUNT         1736 a         1471 c         1384 g         2176 t
ORIGIN      
        1 taagttattt gttgttagtg gttcgttgtc agttgttagt ggttcgttgt cagttaattt
       61 gcaagccaaa ttcattaaaa aatatgatcc tttaagattt ttctttttat ttttacggtc
      121 aaacaatgag ggatgcatag aaaatcgtcc tcttttttcc gccgcaaatc cgttgaactg
      181 attatcgaac atgccgaaga caaagttgaa ggacaaacga gtctgaggcg caatctcggg
      241 gttgccgatc ttaccgctct gggtatagcc gccattatcg gtgcgggtat tttcagtacg
      301 attggtactg cagcagctta tggggggcct gctgtttcac ttttgttcat ctttacggct
      361 atagcttgtg gtttttcggc cctgtgctat gcagaatttt cttcggccat acctattagt
      421 ggcagtgctt atacttacgc ttatgcttca tttggtgaac tcatcgcctg gattattggc
      481 tgggacctca tcatggaata tgctatcgga aacattgccg tagcaatttc ctggtcgggt
      541 tattttactg gattgatgga gggtttgggc atacccatcc ctcaattttt atgtatcgat
      601 tatctgactg cctccagagg ttttcatgag attatcgaat cgggaatatc tgtcgaacaa
      661 gccagccggc aattgcagga agcttttcag gcatggacta cggctccaca gatagcagga
      721 ataaggttga tagccgatat tcctgccttt ggcatcgtgg tgctcattag ctggctggtt
      781 tatgttggaa ttcacgaaac caaggtggct ggaaatttca tggtattgct caaactcatt
      841 actttacttg tagtcatcgg agtgggagca ttttatgtaa atcccgacaa ctggtcgcct
      901 tttgctccca atggaatatc gggagtgcta aaaggtactg ctggggtatt ttttgcctat
      961 attggtttcg atgcaatcag cacgactgcc gaggaagcac gcaatccaag gcaagatttg
     1021 ccccgggcca tgatttatgc cttgctgata actacaatcc tatacatcct catcagcctc
     1081 attctcacag gtatggttcc ttacagtcac ctcgaggtat ccgatcctct ggcctatgtt
     1141 ttttcgtttc atcacatcaa ctggctttcg gggattatta gtttgagtgc tattatagcc
     1201 atggcaggtg tacttttggt cttccaagta gggcaacccc gcatctggat gagcatgagc
     1261 cgcgatggac tgcttcctaa aatcttttcg aaactacatc cccgacatcg cacaccggct
     1321 tttgccacca tcgttgcagg cattcttgtg gccgttcctt cccttttcct caatcttact
     1381 gaagttaccg atctcaccag tataggtact ctcttcgcct tcgtcctcgt ttcgggtgga
     1441 attttagccg tcaatccaca cggcaaaaaa caaaaaaaag acgacatcaa ccgaaaattc
     1501 gtcgtacctt atttgaatag cagaatctgg ctcctaccta tctggactat catgataggt
     1561 agtttgtttt atttccaacc cgaagggttt attaaatctg acggtttgac ttcattgata
     1621 ggaaatatac ccattttatt atttgctatc tgttccatca ttatctcgat atttgccatc
     1681 ttgaaggaat ggtcgctgat acctgtattg ggacttctta ccaacctgta tctgatgtcg
     1741 cagttgggga taactaactg gatgcgattt ttgatatggt tggtgatcgg gttaatcatt
     1801 tattttactt atggatatcg gaaaagccgc ttaaaaggct ggctcaaaca aataaaaaca
     1861 gactgatatg ttgacttggc aggcaattgc attgttcttg ggtggaatat ttgcgggttt
     1921 tgtaaacact ctggctggag gtggaagcat tgtgagcctt tcgctcatgc tcctgtttgg
     1981 tttggatgcc aatgtagcca atgggacaaa tcgtttggcc attatttttc aaagccttac
     2041 ctcaagtgag acttttcggg ttaacaaaat tctggatttt aaacgtggga aagctgctgt
     2101 atggagtgcc ctgattggct cactggccgg tgcctgggta gcagtcgacc tgaataagga
     2161 aatcatcgaa aaaataatta ccatcgtcat gatggccatg ctgttgttta ttttttataa
     2221 tccttcgaga tggctgaagg gagaccctga acgtaatgcc aaaccggcta acctgttcga
     2281 aatctttttg tttctggtca ttggatttta tggtggattt gtgcaggtag gaatcggtta
     2341 tttacttctg gccgggcttg tacttcaagc gggttatgat ttggtaaaag ccaatgcctt
     2401 gaaggttttc atcacttttg tctattcacc ttttgtcttt gccatattcg tatggcatca
     2461 ccaggtgagc tggattcatg gaattccatt agccattggg gcatcaatcg gagcatttat
     2521 tgcctcaaaa ttggcaatta aaaaaggagg agggttcatt cgctggacaa tagtcgtaat
     2581 gatcctgatt acagctcttc aaactttcaa tgtcatcgac ctaaccacct tattctcctt
     2641 tctgaaaccg aaataaaaat tgaaggagag atttttttat tttgtctcgt aaagtttttc
     2701 tctatcgact ttttcgacca tttcattatt cttcagcttg cgcgaaattg ccgaaaaagg
     2761 agctatgatg acgtcttctc cttccttcaa acccgatata atttcgatgt aattgttatc
     2821 ctgaattcct gattttactt ctctgagccg ggctatgcca ttatcgtaaa cgaaaacata
     2881 ttccttgata atttcaggca atttattttc gctttgttcg gtttgtgatt cttcctcggg
     2941 tttggaagct ttacgggtaa ttctacccgt agtatcagaa cgagtagtta ctgcctgaat
     3001 aggaaccgaa agtacgtcga aacgtctttg ggtttgaatc tcggcggtag tcgacattcc
     3061 tggccgaaaa gggctataat cgggctgatc agggggtatt aaatcgagat aggaggattt
     3121 gagcaattga atttttacct gaaaattggt aacttggtct atgctcacgc ccgtggtggt
     3181 ggccgaagta gctacttcgg ttactattcc cttgaatttg cgattcaaat aggcatctac
     3241 ttctatcaat gccgtatcac ccagttttac cttaatgatg tcgttttcat tgacatctac
     3301 ttcggcttgc atctcatcga gattagctat tcgcatgatt tcggtaccgg ccgaaaattg
     3361 tgaagcaccc attacccttt cgcccttttc tacactcagt tttgaaaccc ttccatcggc
     3421 aggcgcataa accgccgtct tcgttagatc ttcacggctt tttttcaggg ctgcattggc
     3481 actgcttatg gaatattccg cagcttttat cgtagcctga attgcatcca aatcggcctt
     3541 ggctacttca taattggtct tggcagtttc aaaatcggca tccgagatta ccttcttctc
     3601 ccaaagcgct ttgttacgca gataaacggt ttcggtattc tgaaaccttg cttccaactg
     3661 agccaactgc gccttggcat tcaccagatt tgccctttgt gagttgagct gagcttctgc
     3721 ctgctcgtaa gccgatttat aaatctccgg atctatgcgt accaaaatat caccaacctt
     3781 tactctgtcg ccttccttta caaaaatttc tattacttct cccgaaatat aaggactaat
     3841 cttaatatcc tttaccggct gaattttacc cgtagcagat atcgcctcaa tgatattccg
     3901 tttttgtacc ttttcggtgg caactcgctg ataattattg tgattctttt tatttacggc
     3961 cagaataacc aataaaatca ctactaaacc cgccccaatt atccaataaa gtcgtttatt
     4021 cttcttcatg ataaaaattt tatgattgat agaataatta attcaatact aatggttttc
     4081 cctcataaaa gtccaaaatc ttgaccttga aaataaaatc atattttgcc tgaataagtt
     4141 ccgactgtgc cttgttgagg tcttccttgt tctggttgta ttcaacggca ttgagcaagc
     4201 ccacggcata tttttcactg gcgtaattaa aactttcttg ctgggcatgt aacttggttt
     4261 cggcggctac atattttttc aaggcagcac ttgcatcggc ataagcttgt acgatgagtt
     4321 tacggagatt gttttgcgac agttgaaggt caagttcggc attggcaact gcaattttcg
     4381 ttcgctgtac attgttcctg gaacggtaac cattgaaaat gggtatgttc aaataaagcc
     4441 caagcgattg attttcattg gcttttaact gatcaccgaa aggttttacc cgataggaat
     4501 attccggtga aattcctatg accgtatccc cggtaagttg agtaatccca atgggataag
     4561 ctatgagagt gggaggcaca ttggggtcct tttcgagtga aactcccgaa taacccgttc
     4621 cccaactgcc gttgagtgca agcgtcggac cataacttcc gcgattgatg tcgaattctt
     4681 ttctggcaga ttctattctg tatttcgaag aaatgatttc gggttgatgc tgcaaggcat
     4741 aattaaccac ctgatcaacg ggcgaaaggc tgatatttac cgaaggagcc gccaactcag
     4801 gagctacaac actaaaaccg cgcgaatctt ccagatcgat gagctgagat aattgcaaca
     4861 atgccagttc ataattattg cgagcttcaa ccaactgcaa ctcggcagat gccacctgcg
     4921 cctccatgtt caacaattca ccctgtgcca gtgaacccga ttctactaaa atctttatcc
     4981 gatcacgctg taatttcacc acctttaact gactctccga tatattcaac aactctgcat
     5041 tatacaaggc ctgaagataa aatgtggcaa cattcagcga tatgtcgttc atcatttttt
     5101 ccacctccag ttcacttata ttcaaatcga gctggctctt acgcaaggtg ttgagcttct
     5161 gaaagccact aaataacacc atttgcgtag aaacataaaa actgttagac cttacccttt
     5221 cggttgcaaa ctgattcgta aatggatcga tggtttgtcc ccaattgtac acatgcgtgg
     5281 cattacctcc caaagtgggc aacatcgtca gcttcgattc cgtcgtatta atcttctgaa
     5341 gctgaacatt caacatttgt ttcttgacgt taatattgtg attgcgggca taggtaatac
     5401 attcttccag cgtccattca tgaatttcct gaccgtaacc taaaaaaggt aaaaagaaaa
     5461 gcagcatcaa taaaatgacc tgcttcaaaa tcttaaagga attcatcata tttagaccaa
     5521 ttttcaaatt gtaaaatcta atttcctgag ttttgataca atttatactt ccacgtttag
     5581 acgataaccg aaaaatgatg ttacattttc aggctcaaaa cgcttcaaag gtatgacttt
     5641 ctggtatagc acaaaagaat gtgagatgaa attatttatt ctccttggca aacatcgata
     5701 aacacccgat gagaacttca tttctttttt tgaattttct tcaagataag tcaatgaatt
     5761 aataagagtg gcttatattt gtgcattgat tcctttagaa ctctatgatg agaaaaattc
     5821 gcatctcgat gattttgttg ctcacgggta ttctatcctc ggtgcaggca cagcaattga
     5881 aaagcgatac cttcgacgtg gtacattatg atctccattt ggacatcatg aattttcagg
     5941 ccaaacagct gaatggattt gccattctca cccttactcc caagatgaat caactttcat
     6001 acatttcact cgatttactg agtcttgagg tggactcggt gagagtggaa ggacagccta
     6061 tcctatcgtg gtatcaggat gacactcttt tgcgcatccc tttgctttcg cctgtatcta
     6121 taggtgacac ttttcaggtg caaattcgtt atcatggcac tcccgttgtc gaacctagcg
     6181 gatggggagg ttttcatttt agctcatgga ttgcctataa tcttggggtt gcttttcaag
     6241 ccaatccaca taactatggt cgagcctggt ttccatgtat cgatgatttt atcgaccggg
     6301 ctacttatga ttattatatt actaccgaag ccggcaaaac tgcggtatgt ggcggcctcc
     6361 tcatcgattc tatcgttcat cccactaaca acattacctg gcattggaaa atgaatcagc
     6421 ccattccagc ttatctcgcc tccgtagccg tggcttccta tactaaaatt gccgatatat
     6481 atactggcat tcaagccgat atacctattt tattatactt caggccttca gatactgcag
     6541 ccgttaataa tctttttgta aatctgaaag atattctatc ggtttatgaa aaccattggg
     6601 gcccttatag cttcgataga gtaggttacg tgggaaccat agagggcgcc atggaacatg
     6661 ctgccaatat tgccctccct gtaagtaccc tttcgtcagg ctatgaatgg ctttacgcac
     6721 atgaactctc acatatgtgg ttcggagata agattacctg ttcctcg
//
LOCUS       DAOORU010000130         6736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_13861_length_6736_cov_6.136357, whole genome shotgun sequence.
ACCESSION   DAOORU010000130 DAOORU010000000
VERSION     DAOORU010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13861_length_6736_cov_6.136357"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="PLF20_08150"
     CDS             <1..1237
                     /locus_tag="PLF20_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX81009.1"
                     /translation="GWISTGFITNEISAYYGSTPSIDGIISPGEWSDAFMVDLSNVLG
                     RYDNIVRPIGSVMGYFKVNENKTALYVAVDNKNDVSLKDHDEVALYIDDNNDGSYPEP
                     GDDSEGNYWAVYYGSGNLIKYRPIYNTGGVGTTIELVNPQIEVSDLSGHVVYEFVLPL
                     GHDYPWQINYNANNESGLFVFVLDDPSYYTGWWPCQNMNIFTPEGYGKITFGAIDELP
                     PPPENLQIEVGFGGKIMLHWTQPTINDFNHFNIYWLAEGSPNFTLLDTTVGVQYFYTV
                     PSSGLYKFYVTTVDNTGHESVPSNIVQANIIIGIDEFILNEDFSSIKIWPNPFEGILN
                     IDIEAMKETNLSIRITDMSGKLIANVFDSRISKGYHHFTWEGKMDNGKLLKPGVYLIN
                     LMSETGYIQTYKLVVMGRK"
     gene            complement(1428..1730)
                     /locus_tag="PLF20_08155"
     CDS             complement(1428..1730)
                     /locus_tag="PLF20_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor"
                     /protein_id="HOX81010.1"
                     /translation="MKVNKLKHFSHKYLFLILFVIFLIWLTFFDPNSLITQIKLSHQE
                     KQMRQEAEWYRKEINNYKTQLNEILYNPEVTEKFVREQYLMKRPNEEIFLIARPVE"
     gene            complement(1727..2293)
                     /locus_tag="PLF20_08160"
     CDS             complement(1727..2293)
                     /locus_tag="PLF20_08160"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOX81011.1"
                     /translation="MILTKEEALKLLNEHVQNPRMIQHCLSSEAVMRALARRLGKDED
                     RWGLAGLLHDIDVEYTNADPLTHGLIGAQWLEDMGIDKDIVDAIRMHNEMAAQKPRTT
                     VFEHALSAGETITGLINATALVYPDKDIQLVKPKSIIKRMKEKAFAASVKRENIMECE
                     DIGLSLEEFITIALEAMQAIPDYNEFNK"
     gene            complement(2253..2810)
                     /locus_tag="PLF20_08165"
     CDS             complement(2253..2810)
                     /locus_tag="PLF20_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOX81012.1"
                     /translation="MESTNTNIKPFIHLREASKDDAPIILELICELADYERAPEQVTL
                     TEAELLNDGFGERPLFKVILAETGGKTVGMALWYFAYSTWKGKVLYLEDIIIKKDFRG
                     MGIGSLLFEELILQAGKHRAKRMQWQVLNWNEKAIRFYQKYGANMSSEWLNGSLTEEQ
                     ILNLKDKIQPFHHDINEGRSFETFK"
     gene            complement(2785..3759)
                     /locus_tag="PLF20_08170"
     CDS             complement(2785..3759)
                     /locus_tag="PLF20_08170"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOX81013.1"
                     /translation="MESLKVISVNISKKKGTAKIPCQQIELYESGIINDAHAGDWNRQ
                     VSMLSIESIHRFEEKTGRKVQPGEFAENITTQGFNILKTQPGDRFFNDQIELEVTQIG
                     KKCHGNSCIIFQEIGDCVMPKEGIFCKVIKPGKIKPGDTLYHNPIASSNSAFYLDEHI
                     FKGKVIILSDRAFHGKYTDKSGPEIIKMLGDFFYKQNWKFTLHSQILPDNAECLKNII
                     TQSFNEKTDLLITTGGTGIGPHDTTVDVVAPFLQKQIPGIMEIIRIKYGTVNPAALLS
                     RSVAGIYDKTLVFTLPGSPKAVREYLNEIFKILPHCFEMLYGIDKHKH"
     gene            complement(3834..4289)
                     /locus_tag="PLF20_08175"
     CDS             complement(3834..4289)
                     /locus_tag="PLF20_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOX81014.1"
                     /translation="MSLEILINNDIKQAMLAKDTRKLEALRAIKAALLLEKTGKDISG
                     GEIPAEVEVKTLQRLIKQRKEAANIYTEKGRQDLADEELYQLAIIEKYLPKQLSEAEI
                     QTRVEEIISRLGASSIKDMGKVMEVAAKELSGQANNKTVSEIVKRLLVT"
     gene            complement(4316..5851)
                     /gene="ftsZ"
                     /locus_tag="PLF20_08180"
     CDS             complement(4316..5851)
                     /gene="ftsZ"
                     /locus_tag="PLF20_08180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOX81015.1"
                     /translation="MLKFEAPKNHSSIIKVLGVGGGGNNAVNHMFDQGIVDVDFIQCN
                     TDAQILEASPIPTRIQLGKSGLGAGNRPDIGRKAAEESIEQIRQILENNTKMLFITAG
                     MGGGTGTGAAPVIASVARELDILTVGIVTIPFSFEGRKRKLQAEQGIEELKKYVDTLL
                     VINNDRLRDFYGNLKLREAFAHADDVLTRAAKGIAEIITVKAYINVDFEDVKTVMQNS
                     GTAIMGSSVASGENRALKAAEEALASPLLNDNDINGARNMLLYISSSKDNEITMDEVT
                     EITAYIQNNAGNDVDVIWGVGYDENLGDAIGITLIATAFEAPKTKQDPKAQVAKEKNI
                     FNLNSEEPIPFVSNPSTEENNESIRLVSQSVPEKTPTANETISQEPSQLKKPAQRNIY
                     PLFPEEENPTAHSPYTIPESSNEPEIAKPFTGTEPSYSISEKVEAKNTERVNVLRGFS
                     YPIKSLDQVEKLENEPAYKRRNKNLEPPPLSSESLAPKSAISDQPGGPEIRSNSYLFD
                     NPD"
     gene            complement(5995..>6736)
                     /locus_tag="PLF20_08185"
     CDS             complement(5995..>6736)
                     /locus_tag="PLF20_08185"
                     /inference="COORDINATES: protein motif:HMM:NF025806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOX81016.1"
                     /translation="EKEAGVVLVDVGGGTTDLAIFQDDIIRHTAVIPFGGEIVTEDVK
                     EGCSIIRKHAEELKIKFGSSLASENRDEDVVSIPGLRGRPPKEITLKNLASIIQARME
                     EIMEYVYREIVNSGLERKLIAGIVLTGGGSQLRHLKQLTEFISGMDTRIGYPNEHLSP
                     RCPSELASPMYATGIGLLLMALERTERDLKKRAVLSQTSTKIQEQPVDVPIFEEKQSE
                     KFVDKEPKKSKRESFLERITKWFEDDGL"
BASE COUNT         1690 a         1286 c         1472 g         2288 t
ORIGIN      
        1 aggttggatt tcaactggtt ttattacaaa tgagattagt gcctattatg gatcgactcc
       61 ttcgatcgac ggcattatta gtccgggtga atggtctgac gcttttatgg ttgatttgtc
      121 gaatgtattg gggaggtatg acaacatcgt caggcctatc ggcagcgtga tgggctattt
      181 taaagtaaat gaaaataaaa cggcacttta tgttgcagta gataataaaa atgatgtatc
      241 gctaaaagat cacgatgaag ttgctctata catcgacgac aacaacgatg gttcttatcc
      301 tgaacctggc gatgattcgg aaggtaatta ctgggctgtt tactatggct cgggtaatct
      361 gattaaatac agacccatat ataatactgg aggtgttggt actacgatag aacttgtaaa
      421 tccacaaatc gaagtttcgg atttaagtgg acatgtagtt tatgaatttg ttttgccgtt
      481 agggcatgat tatccatggc aaatcaacta caatgcgaat aatgagagcg ggttatttgt
      541 gtttgttctt gacgatccat cgtattatac cggctggtgg ccttgccaga atatgaatat
      601 ttttactccc gaaggctatg gtaaaataac ttttggcgct attgacgagc ttcctcctcc
      661 ccctgaaaat ttacagattg aagtaggatt cggaggaaag attatgttgc attggactca
      721 acctactatt aacgacttca accatttcaa catctattgg ctagctgagg gaagtcctaa
      781 ttttacattg ctcgatacaa ctgtgggtgt acaatatttt tatacggttc cttcgagtgg
      841 tctatataaa ttctatgtaa caactgtaga taatacgggt catgaatcgg ttccttccaa
      901 tatcgttcag gctaatatta tcattggaat cgatgaattt atattgaatg aggatttttc
      961 atccattaaa atctggccta accctttcga aggaatttta aatatcgata tagaagccat
     1021 gaaagaaaca aacctttcga ttcgaataac ggatatgagt ggtaaattaa tcgccaatgt
     1081 atttgattcg cgaatttcga agggatatca tcattttaca tgggagggta aaatggataa
     1141 tgggaaactt ttgaaacctg gcgtttatct gattaatcta atgtcagaaa ctggatatat
     1201 ccaaacctat aaattggttg taatgggaag aaagtaattt agataaacat tagaaaattt
     1261 cgaaatcatt gaagaaggga tattgctttt ttatcccttc ttttttggta tattacagga
     1321 acgatgaagg tttgtaataa aaattataaa tcagaatagg ttgaaaacaa aaaaatctga
     1381 tttattttga taatttttgt taaagatttt gtatccttcc attttattta ttcaacagga
     1441 cgagctataa gaaaaatttc ttcgttgggt cttttcatca aatactgttc tcttacgaat
     1501 ttttcggtaa cttcgggatt gtacagaatt tcgttgagct gggttttata gttgttgatt
     1561 tcttttcgat accattcggc ttcctggcgc atttgttttt cctgatgcga taatttgatc
     1621 tgtgtaatca ggctattggg atcgaaaaag gtgagccaga taagaaatat gacaaacaga
     1681 atcaagaata aatacttatg ggaaaaatgt tttaacttat tgactttcat ttgttaaatt
     1741 cattataatc gggtatagct tgcatggctt cgagggcaat agtaataaat tcttcgaggc
     1801 tgaggcctat gtcttcgcat tccataatat tttctcgttt taccgatgca gcgaaggctt
     1861 tttccttcat tcttttgata atggatttag gttttactaa ttgaatgtct ttatcgggat
     1921 agaccagtgc ggtagcgttt atcagtccgg taatggtttc accggctgac agcgcatgtt
     1981 cgaagacagt agtccgaggt ttttgagcag ccatttcatt gtgcattcgt atggcatcca
     2041 ctatatcctt gtcgatgccc atatcttcga gccattgggc acctatgaga ccgtgggtga
     2101 gggggtcggc attcgtatat tctacgtcta tatcatgcag taaacccgca agcccccatc
     2161 ggtcttcatc tttccctaac cgacgtgcta aagccctcat cactgcctca ctcgaaagac
     2221 aatgctggat cattcgggga ttttgtacat gctcatttaa aagtttcaaa gcttcttcct
     2281 tcgttaatat catgatgaaa tggttggatt ttatctttca aatttagaat ctgttcttct
     2341 gtcagactgc catttagcca ctccgaactc atatttgccc catatttctg atagaatcgt
     2401 atagcttttt cattccagtt caaaacctgc cattgcattc tttttgcccg atgttttcct
     2461 gcctgcagaa taagctcttc gaatagcagg cttccaatgc ccattcctct gaaatctttt
     2521 ttaatgataa tatcttcgag atacaatact ttgcctttcc aggtagaata tgcaaaatac
     2581 cacagtgcca tgcctactgt tttacctccg gtttctgcta aaattacctt gaagagtggt
     2641 cgttctccaa atccatcatt gaggagttcg gcttcggtta atgtaacctg ttctggagct
     2701 cgttcgtaat cagcaagttc gcagattagc tcgagaatga tcggagcatc atctttcgaa
     2761 gcttctctaa gatgaatgaa gggtttaatg tttgtgtttg tcgattccat ataacatttc
     2821 gaagcaatgg ggtaagatct tgaatatttc attcaggtat tctcgaacgg cttttgggct
     2881 acctggaaga gtgaacacga gggttttgtc gtaaatacct gctacggatc gcgagagtaa
     2941 agcggcagga ttcacggtac catattttat ccgtattatt tccatgatgc cgggaatttg
     3001 tttttgaagg aagggggcaa caacatctac agtagtatcg tgaggtccaa tacccgttcc
     3061 acctgttgta atcaggaggt cggtcttttc gttaaaagat tgggttatta tatttttcaa
     3121 acattcggca ttgtcgggca ggatttgcga atgaagggta aatttccaat tttgtttgta
     3181 gaaaaaatcg ccgagcattt ttatgatttc cggcccgctt ttatcggtat attttccatg
     3241 gaatgctcta tcacttaaga tgataacctt ccctttgaag atatgttcat cgagatagaa
     3301 agcggagttg gaggaagcga taggattatg ataaagagta tcgccgggtt taatctttcc
     3361 tggttttatt accttacaga aaatgccttc ctttggcatc acgcaatcgc cgatttcctg
     3421 aaaaatgatg catgaatttc catgacattt tttcccaatc tgagtaactt ccagttcgat
     3481 ctgatcgttg aagaagcggt cgccgggttg agtttttaga atgttgaagc cctgtgtagt
     3541 aatattttcg gcaaattcgc caggctgtac tttacgaccg gttttttcct cgaatcgatg
     3601 aatgctttct atactgagca tacttacctg acggttccaa tcgccggcat gcgcatcgtt
     3661 tatgattcct gactcataga gttcgatttg ctggcaagga attttagcag ttcccttttt
     3721 tttgcttatg tttaccgaaa taactttgag cgattccatt ggagttggct tgtttgttta
     3781 tttattgggt atttctatca agatgttgat aatgatttcg atagtttgtg ggtttatgta
     3841 acaagcaagc gttttacgat ttccgaaacc gttttattgt tggcttgacc gctcaactct
     3901 tttgcagcca cttccattac ttttcccatg tctttgattg aagaagcgcc taagcgagag
     3961 ataatttctt cgactcttgt ctgaatttct gcctcgctca gttgtttggg gaggtatttt
     4021 tcgatgatgg caagctggta gagttcctca tcggccaagt cctgacggcc cttttctgta
     4081 taaatatttg ctgcttcttt cctttgtttg atgagtctct ggagtgtttt cacctctact
     4141 tcggcaggta tttcgcctcc ggaaatatct ttcccagttt tttctaaaag aagagcagcc
     4201 ttaatggcgc gaagcgcttc gagcttgcgt gtatcttttg caagcatggc ttgctttata
     4261 tcattattga ttagaatttc gagcgacatt ttattgcaaa ttcaagggtt ggaattcaat
     4321 cgggattatc gaacaaataa gaattggagc gtatttccgg gcctccgggt tggtcggaga
     4381 tagcggattt tggtgccagc gattctgatg aaagaggagg tggttccaga ttcttgttac
     4441 gccgcttata tgcgggttca ttctccagtt tttccacctg atcgagtgat ttgataggat
     4501 agctgaaacc cctcaataca ttcactcttt ctgtgttctt agcttcgact ttttcagaaa
     4561 tcgagtagga gggttcggtg cctgtgaaag gtttggctat ttcgggttca tttgaggatt
     4621 cgggtatagt ataaggtgaa tgagcagtgg ggttttcttc ctcggggaaa agaggataga
     4681 tgtttcgttg agctggcttt ttaagttgtg atggttcttg tgaaattgtt tcattcgcag
     4741 tgggagtttt ttcgggtacc gattggctaa ccaatcttat ggattcattg ttttcctctg
     4801 ttgaaggatt tgaaacaaaa ggaattggtt cttcgctgtt gaggttgaaa atattctttt
     4861 cctttgctac ctgagctttg ggatcttgtt ttgtttttgg agcctcgaaa gcagtagcaa
     4921 taagggtaat tccgatggca tcgcccagat tttcgtcgta acctacaccc cagatgacat
     4981 ctacgtcgtt gcctgcatta ttctggatat aggcagtaat ttcggtcacc tcgtccatag
     5041 taatttcgtt atcttttgac gaggagatgt aaagcagcat attgcgtgct ccattgatgt
     5101 cattgtcatt gagtaaaggt gaggcgaggg cttcttctgc ggctttgagg gctctatttt
     5161 cacctgaggc tacgctcgat cccatgatgg cggtaccgct gttttgcata actgtcttta
     5221 cgtcctcgaa gtcaacattg atataggctt tgacggttat gatttcggca attcccttag
     5281 ctgcacgagt caagacatca tcggcatggg caaaggcctc tctgagtttt agattgccat
     5341 aaaaatccct taacctgtca ttgttgatca ctaaaagtgt gtcgacgtat tttttcaatt
     5401 cttctatgcc ttgttcggct tgcagtttac gtttacgccc ttcgaagctg aagggtatgg
     5461 ttactatgcc cacggtaagt atatcgagct cgcgagctac cgatgcaatt acgggagctg
     5521 ctcctgttcc tgttcctcct cccatcccgg cagtaataaa cagcattttg gtgttgtttt
     5581 ccaagatttg ccggatttgt tcgatggatt cttcggcagc ttttctgcca atatcgggtc
     5641 tgttgccagc cccaaggcct gatttcccga gttgaatcct tgtgggtatt ggactggctt
     5701 ccaatatttg tgcatccgtg ttgcattgga taaaatcaac gtcaactatg ccctgatcga
     5761 acatgtggtt tacggcattg tttcctcctc caccaacgcc cagcactttt atgatagaag
     5821 aatgattttt gggtgcttcg aattttaaca taggttgaat ttgtttaaga tttaaaatta
     5881 attaaagatg aattctttgt gaggtctgat gaattcattg ttttttgaac atttcgagga
     5941 aattcttttg tatggaatgg atagagatat ttcatcttcg atgagaatga aagtttatag
     6001 tccatcatct tcgaaccatt ttgtaatgcg ctccaggaag ctttcccttt tcgatttttt
     6061 gggttcttta tcaacgaatt tttcggattg tttttcttcg aaaataggga catcgacggg
     6121 ttgttcctga attttagtag atgtttgcga taggacagcc ctttttttca ggtcgcgttc
     6181 ggtgcgctcg agagccatca ataataagcc gattcctgta gcatacatgg ggctggccaa
     6241 ttctgagggg catctgggtg agagatgttc attgggatat cctatacgag tgtccatacc
     6301 tgagatgaac tcggtaagtt gtttaagatg gcgtaactgg gagccaccac ctgtgagtac
     6361 gattcccgca atcagctttc tttccagccc cgagttgacg atttctctgt aaacatattc
     6421 catgatttct tccatccggg cctggatgat actggcaagg tttttcaaag taatttcttt
     6481 gggtgggcgg cctcttaatc caggaatcga gaccacatct tcgtctcggt tttcgctggc
     6541 caaagacgat ccgaatttta tcttcagttc ctcggcatgt ttacgaatga tggaacaacc
     6601 ctcctttacg tcttcggtta caatttctcc tccaaagggg attacggccg tgtggcggat
     6661 aatatcgtct tgaaagatag ccaagtcggt agttcctccg cccacatcga ccagaactac
     6721 ccctgcttct ttttct
//
LOCUS       DAOORU010000131         6661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_14068_length_6661_cov_4.854375, whole genome shotgun sequence.
ACCESSION   DAOORU010000131 DAOORU010000000
VERSION     DAOORU010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14068_length_6661_cov_4.854375"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /gene="eno"
                     /locus_tag="PLF20_08190"
     CDS             <1..730
                     /gene="eno"
                     /locus_tag="PLF20_08190"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOX81017.1"
                     /translation="AKVLKKAGYSTNVGDEGGFAPNLKSNEEAIQLILQAIEQAGYKP
                     GEDICIALDPASSEYYIPEENVYHLKKSTGEKLSPQQMIDFWTTWVNKYPIISIEDGM
                     AEDDWDGWKLMTQKLGDKIQLVGDDLFVTNTERLAEGISKGVANSILIKVNQIGSLTE
                     TIDAVNMAYRSAYTAIISHRSGETEDTTIADLAVAMNTGLIKTGSACRTDRIAKYNQL
                     LRIEELLGNVARYPGKNFKYYRVA"
     gene            complement(1081..1332)
                     /gene="rpsT"
                     /locus_tag="PLF20_08195"
     CDS             complement(1081..1332)
                     /gene="rpsT"
                     /locus_tag="PLF20_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOX81018.1"
                     /translation="MANHKSAIKRIKTNQKKRLANRYYSKTMRNSVKNFRKIEDKVTA
                     QKKLPQIVSMIDKLSKKGIIHKNKAANLKSKLMLKVNTL"
     gene            complement(1572..3002)
                     /locus_tag="PLF20_08200"
     CDS             complement(1572..3002)
                     /locus_tag="PLF20_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOX81019.1"
                     /translation="MKIQALYSIFFFSVLMGACEPKTEEFAPSRGEADFYHYVALGNS
                     LTSGYADGALYKSAQAMSYPAILSEQLKKVGGGNFTQPVVDNEDGLFAGKLFLDYSLD
                     CNGEFSLSPVISSGNPIGYPLAIRPVGYSVDNFGVPGAKVAHLIFPGYGNPQRLQQNP
                     PTANPYFVRFASDTGTSVLADAMKQNPTFFTLWIGNNDVLGYATSGGENNTADESITL
                     EGNFHFAYETLINTLTSNGAKGALANIPDITSIPFFNTVPTMGLYLDENSASSLNQAY
                     HQVEIFIQSLGYPDFGYGFQFKTGYNAFIIEDRNFPYQVPKALRVRQAKPDELILFTI
                     PQDSLKCGGMGSYNIEKQRPYGIPQQFVLDEEEIAQIQNAVKRYNAIINELAIQKNLA
                     LVDVANLLQQAKSGITYDGLNFDIKYISGGIFSLDGIHLTPQGAAIVANYFIEAINKQ
                     YKASIPKTEITQYPGIKYPETIPLKK"
     gene            complement(3021..4235)
                     /locus_tag="PLF20_08205"
     CDS             complement(3021..4235)
                     /locus_tag="PLF20_08205"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOX81020.1"
                     /translation="MKKFSLLFIILITSSIGLLAGGYQVRLQGARQSGMGLTGTSLIS
                     GASDIFYNPGSLSFLSKKFEFTAGTNAIFSKIEFRSLESDYVAKTNNPVSLPFFFYGA
                     GKITDKITAGLGIYTPYGSKTDWPDDWSGRFLIQNIALSSIYFQPSISFQILDNLSIG
                     AGLAYVMGSVDLQRALPYNDRSFVKLNGNASAFGFNIGVLFKPAESMHIGIDYRSKIE
                     VKLDDGDANFRIPASLVSIITIENKFSSSLPLPANLDCGLSWQITQKLLFSFEINWVL
                     WNCYDSLTFTFIENGDMLNSINPRLYSNSIIPRFGLEYRIQPKLKIRTGAYYDNSPAN
                     DEYFTPETVTLNTLGFTFGLSYHPIENLNFDFSFVQLMGIEGEKKYSPGNFAGKYRSS
                     ASIPGIGISYSF"
     gene            complement(4859..5293)
                     /locus_tag="PLF20_08210"
     CDS             complement(4859..5293)
                     /locus_tag="PLF20_08210"
                     /inference="COORDINATES: protein motif:HMM:NF015444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOX81021.1"
                     /translation="MEFEQDNELIVIRLWKDEEILSSLTEFCNSHQIKAGWISGIGAA
                     EKIIAGYYDFIRKEYVWKEFEGVHEIVPLTGNISQIGEKVFLHLHINFTDHDLSSYGG
                     HLKSAIVNPTLEVIVCKLTRPLVRFYDIETGLYLLKLSNNNI"
     gene            complement(5306..6346)
                     /locus_tag="PLF20_08215"
     CDS             complement(5306..6346)
                     /locus_tag="PLF20_08215"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HOX81022.1"
                     /translation="MFSMNRFQWRFKIFGLLTIGLTLQSCITQRKISFSTLTPAEITL
                     SPEISEVCLINHGISEPEDSNGVFYGFAGQLYYDSTKYDTLISWAVMDGLTDGFATSQ
                     RLVISGEPLLWLRSSIEKETFAFISDTMMDSFCPSNIADASLIIDKMSIFDNFDYFLM
                     DNGLYYMQLTALAAIGFQFYDLQNKQLVDQYISIDTLSYSTYGYSWEKTIATLPGRAE
                     LYQDIAYDIGKKYAWRLTPGLKKHQRFYYLTNDKDIQKGHEYANQGLWATAARYWIKL
                     TDNKNKRKAYLSAFNMALASEMEGRLDIALYWIDRALEKYHTAEAVKYKTILEQRQKD
                     LDLLIKQMSINP"
BASE COUNT         2080 a         1190 c         1277 g         2114 t
ORIGIN      
        1 ggccaaagtt ctgaagaagg caggttattc taccaatgta ggcgatgagg gcggtttcgc
       61 acctaatttg aaatcaaatg aagaagctat tcagcttatc cttcaggcta tcgaacaagc
      121 tggctataaa cccggtgaag atatttgcat agccctcgac cctgcatctt ccgaatatta
      181 catccctgaa gaaaacgttt atcatcttaa aaaatctacc ggagaaaagc tttccccaca
      241 acaaatgatt gatttctgga ccacttgggt gaataaatac cccattatta gcattgaaga
      301 tggaatggcc gaagatgatt gggatggatg gaaattgatg acacaaaaat tgggcgataa
      361 aatacaactg gttggcgatg acctatttgt cacaaatact gaacgacttg cagaaggaat
      421 cagcaaggga gtagcaaatt ctattcttat taaagtaaac caaattggta gtctcaccga
      481 aactatagat gcagtcaata tggcttatcg ttctgcttat accgccatta ttagtcaccg
      541 ctcgggtgaa accgaggata ctaccatagc cgatctggct gttgccatga atacaggatt
      601 gattaaaacc gggtcggctt gccgtaccga tcgcattgct aaatataatc aacttttacg
      661 aatcgaagaa ttgctgggaa atgttgcacg ttatccagga aaaaatttca agtattatcg
      721 tgtggcataa ggatttctaa tcaaaaaatc gatacatctc ccaatatttt ggaataggta
      781 taaaagactt atttgtaata ctccgagcgt ttgattattc tgttatagca gttaattcaa
      841 acgctttttt tataatttca gtattgggct tattatcagc tcatcttttg tttgaccagt
      901 tttttcaaac cgatagaaaa aatttcgata ggttttaaaa attattccct tggtaattat
      961 aatgaaacga aggggaatgg tcaataatta aagataaaaa aatcccaatt ttatgggatt
     1021 tgataaaaat aggaggcttt taaccttatg atgattcagc aggaattttc ttgaaacttg
     1081 ttatagagta ttgactttga gcatcaattt gcttttcaaa ttagcagcct tatttttgtg
     1141 aatgattcct ttctttgata gcttatcaat cattgaaacg atttgtggga gttttttctg
     1201 tgccgtaact ttgtcctcta tcttgcggaa attctttacg ctattacgca tggtcttcga
     1261 ataatatctg ttggccagtc ttttcttctg attggtttta atgcgtttga tcgcagattt
     1321 atgatttgcc atgtttctcc taattttact tcttcaattt tgggctgcaa aggtaagagt
     1381 tttattttga ttgcaaatga tccttttaaa ttttttgtaa aaatagctcc atgtataatt
     1441 ttgaactggg gtgcaaactg ttggaagaat tttcttgtca aaaagcacca gcttgttgaa
     1501 tggatgaagt ttatttaatt ttacgaaaag tttatgtgga attatctgga tcttatttat
     1561 atactaagct attacttttt caatgggatt gtttctggat atttaatacc tggatattgg
     1621 gtaatttcgg ttttgggtat gctggcctta tactgcttgt ttatggcttc gataaaataa
     1681 ttggcaacaa ttgctgcacc ttgaggggta agatggatac cgtcgagtga aaaaatccct
     1741 ccactgatgt atttgatgtc aaaattcagt ccatcgtaag taatccccga ttttgcctgt
     1801 tgcaacaagt tagctacatc aacaagtgcg agattttttt gtatggctaa ctcgttgatg
     1861 atggcgttgt atcgtttaac tgcgttttga atttgtgcga tttcttcttc atcgagcaca
     1921 aactgttgcg gaatgccata aggacgctgt ttttcaatat tatacgaacc cataccaccg
     1981 cattttaaag aatcctgagg gattgtaaac aaaatgagtt catcaggttt ggcttgccta
     2041 accctcaatg ctttaggaac ctggtatgga aaattacggt cttcaataat aaaggcatta
     2101 tatccggttt tgaattggaa accataacca aaatcggggt aaccaagact ttgaatgaat
     2161 atttctactt ggtgatatgc ttggttgaga gaagaggcac tgttttcatc aagatataga
     2221 cccattgtgg ggacagtatt aaaaaacgga atggacgtaa tgtcgggtat attggctaag
     2281 gcaccctttg ctccatttga ggtcaaagta ttgatgaggg tttcataggc aaagtggaag
     2341 ttgccttcta gagttatgga ttcatcggct gtattattct ctccaccaga agtagcataa
     2401 cctaacacat cgttgttgcc gatccataga gtaaaaaaag tcggattttg tttcatggca
     2461 tcagctaaga cagaagtacc agtatcgctg gcaaatctga cgaagtaagg gttggcggtg
     2521 ggtggatttt gctgcagacg ctgtggattc ccatatccag ggaagataag atgtgctact
     2581 ttggcacctg gaacgccgaa attatctacc gaataaccaa caggtcggat tgccaaggga
     2641 taacctatgg gatttccgct tgatataact ggcgaaaggg aaaattctcc attacagtct
     2701 agactgtaat caagaaataa ttttccagca aaaaggccat cttcgttgtc aactacgggt
     2761 tgagtaaaat tgcctccacc aaccttcttg agttgttctg atagaattgc aggatatgac
     2821 attgcctgag cagatttata taaagcccca tcggcataac ccgaagtaag ggaattccca
     2881 agtgctacat aatggtagaa atctgcttca cctctgctgg gagcaaattc ttcggttttg
     2941 ggttcacacg cccccatcaa aactgaaaag aaaaaaatgg agtaaagagc ttgtattttc
     3001 atcgtggaga aatttttaat ttaaaaacta taagagattc ctatacccgg tatagaggcc
     3061 gatgatcgat acttgcctgc aaaattaccc ggactatact tcttttcgcc ttctataccc
     3121 attaattgta caaacgaaaa atcaaaattc aaattttcaa ttggatgata actgagccca
     3181 aaagtaaatc caagagtatt cagtgttacg gtttcgggag taaagtactc atcgttggct
     3241 ggagaattgt cgtaataagc acccgtacga atttttaatt tgggctgaat ccgatattcc
     3301 aatccaaatc ggggaatgat tgaattacta tataaacggg gatttatact attcaacata
     3361 tcaccatttt cgataaaagt gaatgtcagt gaatcgtaac aattccacaa cacccagttg
     3421 atttcgaatg aaaaaagtaa cttctgtgtt atctgccatg ataatccaca atcgagattc
     3481 gctggcaggg ggagagagga agaaaattta ttttctattg tgataataga aacaagagaa
     3541 gcaggtattc gaaagtttgc atcgccatcg tccaatttaa cctcaatctt tgaacggtaa
     3601 tctattccaa tatgcattga ttctgcaggc ttgaatagaa cgccaatgtt aaatccaaaa
     3661 gccgaagcat ttccattgag cttgacaaat gatcgatcgt tatatggcag tgctctctgc
     3721 aggtcaaccg aacccatcac ataggccagg ccagcgccaa tacttagatt atctagaatc
     3781 tgaaaactta tagaaggttg aaaataaatg gagctcagtg caatattttg aatgagaaag
     3841 cgaccgctcc aatcatcagg ccagtctgtc ttgctgccgt aaggagtata aatacctaac
     3901 cctgcagtta tcttatcggt aatcttacca gctccataga aaaaaaatgg cagactcaca
     3961 ggattgttag tcttggcaac ataatctgat tctaaggaac gaaactcgat tttggaaaaa
     4021 atggcattgg taccagcggt aaattcaaat tttttcgata aaaaacttaa actaccggga
     4081 ttatagaaaa tatcgcttgc ccctgagatg agcgaagtac ccgtaaggcc cattcctgat
     4141 tgacgggctc cctgcaacct gacctgatat ccgcctgcaa gcaggcctat actgctcgtt
     4201 attaagataa taaaaagaag actaaatttt ttcattcgaa ggggaattaa tggatcagaa
     4261 ggcaaaaata tcaaaaaaaa ttccagcaaa ttttcataga acgcggtatg ggttatgata
     4321 tgatctcaaa aataatggag gtctccatac accgctcgct tcgctcgcgg cactcgacgt
     4381 ccggggtggt ttaattaaat aaagttctgg tatattgact aaaatgttaa attgaagttc
     4441 tttgattttg gatatataat catattaatg aatgatataa acttctggaa aagtgataag
     4501 atatttatct tttatcaatc cattcattaa gttttaggac atcgaagcta ccttatcttt
     4561 ctaagtatac ctaaaaccga atcgaaatat ttatatactg aatttcaatg ttttacaatt
     4621 atctttataa tatttttata aacactgtta aaccataagg aggttattgc ctcaaaagaa
     4681 gtgatgtgtt aaaagaactc atggttttgg ttacaacaac taaccgttgt tgtggaattt
     4741 agacacatca ggatggaata catacctgta cttttttctt acaggatttg gttaaagact
     4801 acttaactac cccccagttg cgaaactcgg gggtttgttt ttatcatctt acgataaatt
     4861 aaatattgtt attggataat tttagcaagt ataaacctgt ttctatatcg tagaatctga
     4921 caagaggacg agtgagcttg caaactatta cttcgagggt gggattcaca atggcacttt
     4981 ttaaatgccc tccataagag cttaagtcat gatcggtgaa attgatgtgt aaatgcaaaa
     5041 acaccttttc ccctatctga ctgatatttc ccgttagggg gacaatttca tgcactcctt
     5101 cgaattcttt ccaaacatat tctttcctga taaaatcata ataacctgca ataatttttt
     5161 cggctgcacc aattccagaa atccaacctg ccttgatttg gtggctgtta caaaactcag
     5221 tcagggacga tagaatctcc tcgtccttcc agagtcttat cacaattagc tcattatctt
     5281 gttcaaattc catttttgaa aataattaag ggttaatgct catttgttta atcaataaat
     5341 ctaaatcttt ctgtcgttgt tcgagtatgg tcttatattt cacagcttct gcggtgtggt
     5401 atttttcgag ggctctatct atccagtaga gggcaatatc gagccgacct tccatttcgc
     5461 ttgccagagc catgttaaaa gctgataaat atgccttgcg tttgttttta ttatcggtca
     5521 actttatcca gtatcgggca gcggtagccc aaagaccctg attggcgtat tcgtgtcctt
     5581 tttgaatgtc tttatcatta gtgaggtaat agaatctttg atgctttttt agtcctggcg
     5641 tgagacgcca tgcatatttt ttcccaatat cgtaagcaat gtcttggtaa agttctgctc
     5701 ttcctggcag agtggcgatg gttttttccc atgaataacc ataagtcgaa tatgaaagag
     5761 tgtctatcga aatatattgg tcgactaatt gtttgttttg caagtcgtaa aactggaaac
     5821 ctattgctgc cagagcagtt aattgcatat aatacaatcc gttatccatc aaaaaatagt
     5881 cgaaattatc gaatatactc atcttatcga tgatcaatga agcatcggca atgttggaag
     5941 ggcaaaaaga atccatcatt gtatcggaaa tgaatgcaaa agtttccttt tcaatactgc
     6001 tacgtagcca taaaagaggt tcccctgata ttaccaatcg ctgcgaggtt gcaaaaccat
     6061 cggtcaaacc atccattacc gcccatgata ttaaggtatc gtatttggtc gaatcataat
     6121 atagttgtcc ggcaaatcca taaaaaactc cattcgaatc ctctggttca gaaataccat
     6181 gattaatcag acaaacttcc gatatttcag gagatagggt aatttcggca ggtgtcaaag
     6241 tcgagaagct gattttgcgc tgtgtaatac aactttgtaa agtaagccca atggtcagaa
     6301 gaccgaaaat cttaaatctc cattggaagc gattcatcga aaacatgaaa caatttttaa
     6361 gcataatgaa gatgcaagtg aaatttgttt tatacgaagt taaatgtaat tttgataaac
     6421 aaaggaataa aacattgcat aattttgcga attagtattt aaaatactta ttttgaatac
     6481 attatcgatc gtccttattt agaattcttt taaattactg ttattttatt aacattgtta
     6541 tttttttaac aaataaagaa aacttctaat tatctttgca caggaaattt aagttgaaga
     6601 tttagcaatt aagaaattga ttgtgttaat tttaacatca taaaccttaa aagtatccac
     6661 c
//
LOCUS       DAOORU010000132         6573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_14283_length_6573_cov_6.256214, whole genome shotgun sequence.
ACCESSION   DAOORU010000132 DAOORU010000000
VERSION     DAOORU010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6573
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14283_length_6573_cov_6.256214"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..2682)
                     /gene="priA"
                     /locus_tag="PLF20_08220"
     CDS             complement(202..2682)
                     /gene="priA"
                     /locus_tag="PLF20_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOX81023.1"
                     /translation="MKQRATYFADILLPLAVEGYFTYRIPNDLAKDIKLGMRVVVQFG
                     KQKFYTGLVRRIHQNIPKDYTPKYVLSIVDPYPLVSEVHFQFWEWLASYYMANVGEVM
                     NVALPSSLKLASETRLALHPDFDKDYSGLTEKEMMVAEACEIQKQITLADVARITEMA
                     SVFGLIKKLIDKKVIVLEEELQNRFQPKTRIYLSLHPDYKDNDEKLNKIFNELEKRAP
                     RQSEALLMFLSMTRDREEISRNSLQAMANLGPSPVDGLIKKGIFVSKEHVVSRLGELY
                     KGETQEVRLTPLQQKVYESIRKQWSEKPTVLLYGITSSGKTEIYIKLATEIIQQGGQV
                     LYLLPEIALTAQIVNRLRRFFGDDVAVYHSKFNENERAEIWNRVTENPLYDLKPFKLV
                     VGTRSALFLPFSNLKLIIVDEEHDASFRQAQPAPRYNARDAAIYLAGLLHARTLLGSA
                     TPSLESYFNAQSGKYGFAQILTRYSDIMMPEIILADLREEKNKGASKSLFSQTLLQLI
                     SEALSQKEQIILFQNRRGYAPRIECGKCNWIPLCKNCDVSLVYHKEYHQLQCHYCGYT
                     APLPTECSVCHHTALYLRGFGTEKIEDELSIYYPEARIARFDLDSTRNKNAHVDLIQR
                     FEDRDIDILVGTQMVTKGLDFDHVSLVAILNADNMLAYPDFRAFERGYQLMAQVAGRA
                     GRKNKRGKVVIQTYNPQHPAILFVLSNDYEGMAHQQLSERAHYHYPPFTRLILVELKY
                     ADQKNLFEGADHLAKLLRDELGKKVMGPEQPVISRIKGLFIYQILIKLDRNHLLNANK
                     EFIHRTIRQFQTEKEFRRFIVQIHVDPM"
     gene            complement(2687..3919)
                     /locus_tag="PLF20_08225"
     CDS             complement(2687..3919)
                     /locus_tag="PLF20_08225"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOX81024.1"
                     /translation="MLTFAKKFIPLIPPNTHNFVAKTLAGLEEVLEQELKELGATHTQ
                     ILTRAVSFEGTRETVYRTNYHCRTAIKILVPLIQFKASNNEELYEGVRSFQWDQIMNF
                     RDTFMVETVLVHSAFQHSHFISLKTKDAIADWFTNKYKRRPNVDRENPDIRLSLYIHR
                     DECTLYLDSSGDPLFKRGYRMAQGPAPMSEVLAAGLIKLSGWNGEVNFVDPFCGSGTL
                     LTEAALIAHNLPAGLFRKSFGFMHWRDFDRTLWNDIKADARKHKRNQECEIFGSDVSE
                     EAIAIASRNIHRAGFSSEITLKKTRFEDTSFDGPGVLITNPPYGERLQTNDIVALYKS
                     IGDTLKHNYKGFDAWIITSDFFALKNLGLHPTKKVILYNGPLECRFAHFEIYEGSRKQ
                     KNDLFNTLETLPDTSIEE"
     gene            complement(4676..6349)
                     /gene="ettA"
                     /locus_tag="PLF20_08230"
     CDS             complement(4676..6349)
                     /gene="ettA"
                     /locus_tag="PLF20_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOX81025.1"
                     /translation="MAEDHKIIFSMVGVSKIAPPNRQILRNIYLSFFYGAKIGIIGLN
                     GAGKSSLLRIIAGADTSYQGEVVYSPGYTIGYLEQEPRLDDDKTVREIVEEGVLQIVN
                     LLHEYEEVNNRFTEPLNEDEMTRLIERQGELTELIDHFDGWELDNRLEKAMDALRCPE
                     PSLLVRNLSGGERRRVALCRLLLSQPDILLLDEPTNHLDAESIEWLEHHLQNYKGTVI
                     AVTHDRYFLDHVAGWILELDRGEGIPWKGNYSSWLEQKTQRLAQEEKSESRRRKTLER
                     ELEWVRMAPRARQAKSKARLNAYDKLLDEDIAQKEERLEIFIPNGPRLGNKVIEAQHV
                     SKAYESKLLFEDLNFSLPPAGIVGVIGPNGAGKTTLFRLIMQQETPDSGKFEIGESVK
                     LAYIDQVHSPINPDKSVFEVVSEGNEQFYLGGRIINARAYLARFNFTGADQGKKCGVL
                     SGGERNRLHLALTLRSEANVLLLDEPTNDIDVNTLRALEEGLLNFAGCALIISHDRWF
                     LDRVATHILSFEGNSQVYFFEGTYSEYEENKRKRIGDDNPHRLRYKKLQ"
BASE COUNT         1734 a         1358 c         1515 g         1966 t
ORIGIN      
        1 aactgacaat taacaaccaa caagcttcaa tcactaactg ctatctacaa tcccccacca
       61 atcactattc accatccacc atccaccatt ctccctatct cctcgtctcc tcgtctccct
      121 tcctcccttt ttcctttata aactttcaac tttcaacttt taactcatcc atccaccaat
      181 caccatccac tattcatttt tttacatggg gtcaacatga atttgaacga taaaacggcg
      241 aaattctttt tcggtttgaa attggcgtat ggttcgatga ataaattctt tatttgcgtt
      301 gagaagatga tttctgtcga gtttgataag gatctggtaa ataaataagc ccttgatacg
      361 agaaatgact ggttgttccg gccccattac ttttttccca agttcatcac gcagcagctt
      421 tgccagatgg tcagctcctt cgaatagatt tttctggtca gcgtatttta gttcgacaag
      481 aatcaagcgt gtaaaaggag gataatggta atgagctcgt tcactcaatt gctgatgagc
      541 cattccctcg taatcgttcg acaagacgaa aagtatggca gggtgctgag gattataggt
      601 ttgaataaca acttttcctc ttttgttttt tcgtcctgcc cggcctgcca cctgtgccat
      661 caattgataa ccccgttcaa aagctctgaa gtcgggataa gccagcatat tatcggcatt
      721 taagatggct actagtgaaa cgtggtcaaa atcgagtccc ttggttacca tttgcgtacc
      781 aaccagaata tcgatgtctc tgtcctcgaa acgttgaata agatcgacat gtgcattttt
      841 atttctcgtc gaatcgagat cgaaacgcgc aatacgtgcc tcgggataat agatggaaag
      901 ttcgtcttct attttttctg ttccaaaacc tctcaaatag agtgcggtat ggtggcaaac
      961 ggagcactca gtgggcagag gtgccgtata accacagtaa tggcattgta gttgatgata
     1021 ttctttatga tagactagag aaacatcgca gtttttacaa agcggaatcc agttgcattt
     1081 tccacattcg atgcgagggg cataacctct tcggttctga aacaggataa tttgttcctt
     1141 ttgagaaagg gcttcggaga ttagttgtaa taaagtttgt gaaaataaag atttcgaggc
     1201 tcctttgttt ttttcttctc tcaggtcggc caagatgatt tcgggcatca tgatgtcgct
     1261 ataacgagta agaatttgtg cgaagccata tttacccgat tgagcattga aatagctttc
     1321 gagtgaagga gtggccgaac ccagaagggt tcgtgcatgt agcaagccgg caagatagat
     1381 ggcggcgtcc cgggcgttgt agcgtggtgc aggttgtgcc tgacgaaatg aagcatcatg
     1441 ttcttcgtcc acaatgatga gtttcaggtt cgaaaaagga agaaacaaag ccgaacgtgt
     1501 tcctacgaca agtttaaagg gttttaggtc atagagtgga ttttcagtaa cccggttcca
     1561 tatttcggct ctttcatttt cattgaattt ggaatgataa acggctacat cgtcgccaaa
     1621 gaatcttcgc agtcggttga ctatttgagc tgtgagggca atttcgggaa gcagataaag
     1681 cacttggcct ccctgctgaa ttatttcggt ggctaatttg atgtaaatct cggtcttgcc
     1741 gctcgaagtt attccgtaaa gtaaaaccgt tggtttttcg ctccattgtt ttcgaatgga
     1801 ttcatagact ttctgctgaa gaggagttaa tcttacttcc tgtgtttcac ctttgtaaag
     1861 ttcacccaag cggcttacca cgtgttcctt gctcacgaat attccttttt tgatgaggcc
     1921 atctacggga gaaggtccaa gattcgccat ggcctgaagg ctgttgcggc taatttcttc
     1981 cctgtcgcgt gtcatactca gaaacatgag caaggcttca ctctgacgag gtgctcgttt
     2041 ttctaattca ttgaagattt tgttcaattt ttcgtcattg tctttgtaat cagggtgaag
     2101 tgatagatag atccttgttt taggttgaaa acggttttgg agttcttctt cgagtacgat
     2161 gacttttttg tcgatcaatt tcttgatcaa gccgaataca gaggccattt cggtgattcg
     2221 tgccacgtcg gccagtgtaa tttgtttttg gatttcgcag gcttcggcca ccatcatttc
     2281 tttttcagtc aggccactat aatctttgtc gaaatcgggg tggagagcga ggcgtgtttc
     2341 gctggccagt ttcaaggagg aaggtagagc cacattcatc acttcgccca cattggccat
     2401 ataataagaa gctaaccatt cccagaactg aaaatgcact tcgcttacca agggataagg
     2461 atcgactatc gaaagaacgt attttggcgt gtaatctttg gggatgtttt gatgaatacg
     2521 tcggaccaat ccggtgtaga atttctgttt accgaactga actaccaccc tcattcctaa
     2581 tttaatatcc ttggcgaggt cgttggggat gcggtaagta aaatatccct cgacggccag
     2641 gggaagtaaa atatcggcaa aatatgtggc acgttgtttc atgttttcat tcctcgatgg
     2701 aggtatcggg aagagtttct aaagtattga aaaggtcgtt tttttgtttt ctgcttccct
     2761 cgtaaatttc gaagtgagca aagcggcatt cgagaggccc attatagagg ataacttttt
     2821 tggtagggtg taagcccaga tttttcaatg caaagaagtc ggaagtaatg atccaggcgt
     2881 cgaagccttt atagttgtgt ttgagagtat cgcctatgct tttgtacagg gcgacgatat
     2941 cattggtttg caatctctcg ccataaggag gattggtaat cagaacgccg gggccatcga
     3001 aggaagtatc ttcgaaacgt gtttttttca aggtaatctc gctggaaaat ccagcacggt
     3061 gtatgtttct cgaagctatc gcgatagctt cttcggagac atcgctgccg aagatttcac
     3121 attcctgatt tcgtttatgc ttacgggcat ctgccttgat atcgttccag agtgttcggt
     3181 cgaagtcgcg ccaatgcatg aatccgaaac tttttctgaa aagtcctgcg ggcaagttat
     3241 gagcaataag tgcagcttcg gttaatagcg ttccgctacc acaaaaggga tccacgaaat
     3301 tcacctcccc attccacccc gatagcttaa tcaggccggc ggccagaact tcgctcatgg
     3361 gtgcaggtcc ttgagccatg cggtaacctc gtttgaacag ggggtcgccc gaactatcga
     3421 gatatagcgt gcactcgtcg cgatgaatat ataaactcag acgaatgtcg ggattttcac
     3481 gatctacatt cggtcttctt ttgtatttgt tggtaaacca atcggctatg gcatctttgg
     3541 ttttcagcga gatgaagtgc gagtgctgga aggctgaatg aaccaggacg gtttccacca
     3601 taaaagtatc tctgaaattc atgatctggt cccattggaa agaacgcacg ccttcgtata
     3661 attcttcgtt gttgcttgct ttgaattgaa tgagaggaac aaggatctta atagccgtcc
     3721 ggcagtggta attcgtgcga tagacggttt cacgcgtgcc ttcgaaagaa actgctcggg
     3781 taagaatttg tgtatgggtg gctccaagct cttttagttc ctgttccaga acctcttcga
     3841 ggccggcaag agttttggct acaaaattat gggtgttagg gggtatcaaa ggaatgaatt
     3901 tttttgcaaa ggtaagcact tatgcctgat agaatttttt tcatttctct catagcggtt
     3961 tcatgcatcg atattgcttc actcattttg tggatggttt ttttcaggat caaaatcaaa
     4021 acaaaaatgg aaacgagtat aaaaatgagg ataaagattt gatgggagtt ctatagggat
     4081 ggtgattcga tgaaaacagc gaggctacga ggttgggtta cagccagaaa aaatagaaaa
     4141 attcgtgaga atatgcctga cattgtaccg gcaaagtaat ttgcaagatg aatctccatg
     4201 cataacaaag caaaggtggt catggcaaag gcgaggcagg agagaggcaa cagaagaaaa
     4261 attgaagaaa ttgttgattg gaaagatagt caaagaatct ataaagtgct ggatcgataa
     4321 aagtagtgga acccattgcc agtttattgg ttcctttgcc aaaagcgata tctatccagg
     4381 cagcctatat acccacatca tggcaatcca gtccatccaa aatagcaacc cgaaattacg
     4441 gcgataaaag ttctgagagc aagagcgaac tacatagtaa atgaagagaa gagatagcat
     4501 agggttgcat tgcagatgtt agaggtgatg gaaggcgtgc tactgtttaa ggccaagaaa
     4561 aatgaaggag aaatgatgtc gggtaatacg acgggaatgc ctattgaacc aaactctgtt
     4621 ttttggtttt tagggtgagt aaaggttttt tcatagagag aaaccaaaca agaaatcact
     4681 ggagtttttt gtatcggagt cgatgggggt tatcgtcgcc tatgcgtttt cttttgtttt
     4741 cttcatattc gctgtaggtt ccttcaaaaa aataaacctg agaatttcct tcgaaagata
     4801 gaatatgggt agctacgcgg tcgaggaacc agcggtcgtg cgagataatc aatgcgcagc
     4861 cggcgaagtt gagcaagcct tcttcgaggg cacgcaaggt attcacatcg atatcgttgg
     4921 taggttcatc cagaaggagc acgttggctt cagagcgcaa ggtaagtgcc agatggaggc
     4981 ggtttctttc gcctcccgag agaactccgc atttctttcc ctggtcggca cctgtgaagt
     5041 taaaacgcgc cagataagct cttgcattga taatccttcc gcctaagtag aattgttcat
     5101 ttccttcgct caccacttcg aaaacgcttt tgtcgggatt gataggagag tgaacttggt
     5161 ctatgtatgc cagttttacg ctttctccta tttcgaattt tccagaatct ggtgtttcct
     5221 gctgcataat caaacgaaag agagtagttt ttcctgcgcc atttggtcct attactccaa
     5281 ctattccagc gggaggaaga gaaaagttca gatcttcgaa taacagcttg ctttcataag
     5341 cctttgacac atgctgagct tcgatgactt tatttcccag acgagggccg ttaggaatga
     5401 atatttcaag gcgttcttct ttttgtgcaa tatcttcatc gagaagttta tcgtaagcat
     5461 ttaagcgggc cttggatttc gcctgacgcg cacgtggagc catacgaacc cactcgagtt
     5521 ctcgttcgag ggtttttctg cgtcgacttt cgcttttttc ttcctgagcc aaacgttggg
     5581 tcttttgttc gagccatgaa gaataattac ctttccatgg gataccttct ccacggtcga
     5641 gttcgagtat ccagccggct acatgatcga ggaagtatct gtcgtgagta acggctatta
     5701 ctgtgccttt gtagttttgc agatgatgct cgagccattc aatgctttcg gcgtcgaggt
     5761 gattggtggg ctcatccaga agaagtatat cgggttgact gagcagcagg cggcacaaag
     5821 ccactcttcg tctttcacct cccgaaagat ttctgaccaa taaggatggt tcggggcaac
     5881 ggagagcatc catggccttc tcgagccggt tgtcgagctc ccatccatcg aaatgatcta
     5941 tcaactcggt aagctctccc tggcgctcga ttaatcgggt catctcgtcc tcgttcagag
     6001 gttccgtaaa acgattgttt acttcttcgt attcgtggag caagtttacg atttgcaaaa
     6061 ccccttcttc tacaatctcc cgaaccgttt tgtcgtcatc cagccgaggc tcctgctcaa
     6121 ggtatcctat ggtgtaacct ggcgaataca caacttctcc ctgataggaa gtatctgctc
     6181 cggcaatgat tctcaacagg ctcgacttgc cagcaccgtt taatccgatg atgccaatct
     6241 ttgccccata aaagaaggat aaataaatgt tcctaagtat ttgccggttg ggtggggcaa
     6301 ttttgctgac acccaccata gaaaagatga ttttgtggtc ttcggccatt attcgaaatt
     6361 tcatgcaaaa gtaagataat gtgaataaaa ggagtatttt ccttaatttg agtgtaagaa
     6421 tccgagcgac tcccctgtag ttttgtttgt cataaatata tcttttgccg aaatattaaa
     6481 cataaatagc aatattttct ctataaattt cctaatgttt tttttctgaa ggggcttata
     6541 atagacattt attaaatatt tagaacaaag tac
//
LOCUS       DAOORU010000133         6569 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_14295_length_6569_cov_4.222751, whole genome shotgun sequence.
ACCESSION   DAOORU010000133 DAOORU010000000
VERSION     DAOORU010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6569
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14295_length_6569_cov_4.222751"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="PLF20_08235"
     CDS             complement(<1..1302)
                     /locus_tag="PLF20_08235"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOX81026.1"
                     /translation="MKKFIFGWAGLVFILMLASCEDFLELDPVSNGIAVGNAAGDSIL
                     YKTGAEVEAALAGAYADFKNEYYQLDYYVNGDAQSDDAYAGADNPANFQIDDYKIDAL
                     NSNVARDWAYLYAHIGKTNAVINNVDKCPDASLSAARRKEIKAEALFIRAFQYFQLVQ
                     LWGDVPLQLTEVRTISAANLEEIYPLLYPPRTLLDMVYQQIISDLATAARGVKVTAPH
                     KGYATKGAVYTLLAKVYATIEPHDYNKVNLYCDSVMALGYSLMPQYDQLWDNSQENCS
                     ESIFEINYNGGVSDGNWGAKMFRGLDWKKFNIPSNDLVKIFEQEGDMIRFTSSIVWED
                     VSGRWTDPHWPQVHYPFINKWRNYAEGSDQNYIIYRLADVMLLKAEALNEMGDVSGAA
                     SLVNQIRARVNLPETQANYQTSMPLAIEKERRLELAFEGHRW"
     gene            complement(1326..4262)
                     /locus_tag="PLF20_08240"
     CDS             complement(1326..4262)
                     /locus_tag="PLF20_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX81027.1"
                     /translation="MKHFTLLLITLIAFSGSVWGQKKMVTGKVTDEKGASIPGVTIQV
                     KGTYTGTVTDLNGNYKIEVEDKDILIFSFVGYNKQEIPVGSQMVINVVLSEETTILNE
                     IVVIGYGAQQKKDLTTAVAVVNEKDIRNRPIITAAQALQGKAAGVQVIQPSGKPGQQL
                     AVRVRGATSVLAGNDPLYVVDGVPTTDIRGLNPNDISSMTILKDASSSAIYGARAANG
                     VVLITTKRGQEGEPLVTFNTYFGISNLRKNIEVLNTKQYRDLMEEILPGGLDPSMTYF
                     NNWPDYVFGTGYTQSYQFSVSGGSAKSKYYVAGGYLSDKGIVKPARFDRYNVRINLDS
                     EMKSWLKLTSNINVLGIDTKDTPDNLSSGRGGVIMSALNTPPFLHVYKQDGSGQYDPN
                     PFQPSWENPAAYMYGPDQLSTDYQVFGNIGLEARLIKDLYFKTHVGTDITTHQWDYYL
                     DPFRTNYGRNMNGIGRSDKFNNLTWLWENTLDYKFTADQHNFTALAGASIQKFQGNDS
                     YIEGNDFPADTNVRTLWAANNITAGTTREEWALASFFGRVTWDYLSKYYLTMSIRHDG
                     SSKLAHHWGTMPSFSAGWRVSSEEFMKNLTFIDDLKIRGGWGKNGNQEGIPNYARYGL
                     INYYRREITNPLSGPAAVQITYGNPDLKWETTAQTNIGFDLTMFRSRVTLNFDYYYKK
                     TSDVILDVQLPNSLPITSIQTNAGEIENKGIELNLNTINVDKNWKWTTDFNIAFNTNK
                     VLSLKFTDVYYFGRIYSNNSDVVILRTGLPLGSFYGYISEGVNPETGDLVYKDVNNNG
                     ILDPGDRTVIGDPNPDFIFGLTNSFSYKKWDFSFFFQGSVGNDIYNATRIDLEGMFDS
                     KNQSTAVLNRWTPENRNTDIPRAIGGGNMDNVRNSTRFVEDGTYVRLKSLTLSYKIID
                     KPSKKTGISKLSVYLTGNNLLTFTGYSGFDPEVNAYGNSAVEVGIDYGTYPQARSLML
                     GLNVEF"
     gene            complement(4384..6144)
                     /locus_tag="PLF20_08245"
     CDS             complement(4384..6144)
                     /locus_tag="PLF20_08245"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOX81028.1"
                     /translation="MKTIRKFTAMSLAVMALLSLTCLTSCKDDEDENLVIAGFTYQVD
                     SSNFLKVSFTNTSQNYTTCNWNFGDGQTSSEVNPVHIYAAEGEYTVTLTAKDANGNQD
                     VYSTKIVLSDPNVLLTALTGMESKTWKLIRDVTTGRYPLECGPEDHSQIWWAMGRDNN
                     ELANRPCQLNDEWIFYRDGRMEFRAQGDYWAEGGLFSPENQCNTTDQMVGPNGEDLSA
                     WGDGNHTFKLTVGNPSKIKAIGKGAYIGFYKLGNGTEVKTPQDYVEYNIVKLVDGTVD
                     TLIVEGIYRWDPASSGGYWRFVLVHYDNPADEPPIPGPKPEASFSMQMDGRTVTFTNT
                     TENGETYLWDFGDGNTSTEKDPVHTYASDGIYMITLTASNPNGESTVTKQAFVTGTEL
                     TAELLMGGAWRPRAENLSIFVGPGLGDYSWWSVPKNFLDGSTSGTDDWSCITDDEFTF
                     ADGGVYIYNTNGSARNDGYMSSPNGCWADEQIAASGNGAAFGSGTHSWEFVPASGGSR
                     PLIILTNGPTGAAFLGFYKGYYGGENSDGANPPNGGNLTNRYEVMGYANTGTKEYLFV
                     SVDISADHSGSSAWSVILER"
BASE COUNT         1876 a         1411 c         1324 g         1958 t
ORIGIN      
        1 ccagcgatgc ccttcaaagg ccaattctag ccgacgttcc ttttcgatgg ccaagggcat
       61 ggaagtttga taatttgctt gagtttcggg taaattaact cttgcacgaa tctgatttac
      121 tagacttgcg gctccactta catcacccat ctcgttcaag gcttcagcct ttagcagcat
      181 tacatctgcc aaacgataaa taatgtaatt ttggtcgctt ccctcggcat aatttctcca
      241 tttattgatg aagggataat gaacctgtgg ccagtgagga tcggtccacc gccctgaaac
      301 gtcttcccaa acgatagatg aagtaaaacg aatcatgtcg ccttcttgtt cgaagatttt
      361 tacgagatcg ttgctgggaa tattgaattt tttccagtcg aggccacgaa acatttttgc
      421 tccccagtta ccatccgaaa cgcccccatt gtaattgatt tcgaagatag attcggaaca
      481 gttttcctgc gaattatccc acaattggtc atattgaggc ataagtgaat atccgagcgc
      541 cattacactg tcgcagtaaa ggtttacctt gttataatcg tgtggctcga tagtggcata
      601 aaccttggcc agaagagtat agactgcacc ttttgtggca taacccttat gaggagcagt
      661 aacttttact cctctggcag ctgttgccaa atcggaaata atttgctgat aaaccatgtc
      721 aagaagcgtt cttggaggat acagcaaagg atagatttcc tcgaggttgg ctgcactgat
      781 agtacgtact tcggtgagtt gaagaggcac atctccccac aactgtacga gttgaaaata
      841 ctggaaagct ctgataaaca atgcttcggc ctttatctcc tttctgcgag ctgccgataa
      901 cgaagcatca ggacatttat cgacattgtt gatgacagca ttggtttttc caatatgtgc
      961 atagaggtaa gcccagtctc tggctacatt cgaattcagt gcgtcgattt tgtaatcgtc
     1021 gatttgaaaa tttgcaggat tatcggcgcc tgcataagca tcatccgact gagcatctcc
     1081 atttacgtag taatccaact gataatattc attcttgaaa tcagcataag cgccagcgag
     1141 ggcagcttca acctccgcac cagttttgta caagatagaa tcgcctgcag cgtttcctac
     1201 ggctatccca ttggatactg gatcgagttc aagaaaatct tcacaggagg ccagcattag
     1261 aataaataca aggcctgccc atccaaatat gaattttttc atagtatttt tcctcctggc
     1321 attcattaaa attctacatt taatcctaac ataagcgaac gagcctgtgg ataagtacca
     1381 taatctatac caacttccac agcactgttg ccataagcat tcacttcggg atcgaaaccg
     1441 ctatacccgg taaaagttaa aagattgttc ccggttaaat aaacagagag cttgctgatg
     1501 cctgttttct tggaaggttt atctatgatc ttataagaca gggtgagcga ttttaaacga
     1561 acataagttc catcttcaac aaaacgagtc gaattgcgca cattatccat gttaccccct
     1621 ccaatggctc tcggtatatc cgtattacga ttttcgggag tccagcggtt taacacagca
     1681 gtggattgat tcttgctatc gaacatacct tccagatcga tacgcgtggc attgtaaata
     1741 tcattgccca cactaccctg aaaaaagaag ctgaaatccc atttcttata tgaaaaacta
     1801 ttggtcaatc caaaaataaa gtcaggattg ggatcaccaa taacggttct atctccagga
     1861 tcgagaatac cattgttatt tacatcctta taaaccagat caccggtttc gggatttaca
     1921 ccttctgaaa tatagccata gaaagagcct aaaggcaaac cagtacgcag aataactacg
     1981 tcggagttgt tgctgtatat ccttccaaag taataaacat cggtaaattt aagactcagg
     2041 actttattgg tgttaaaggc gatgttgaaa tcggtggtcc atttccagtt cttatcaacg
     2101 ttgatggtat tcagattcaa ttcaattcct ttgttttcga tttctccggc gttggtttga
     2161 atggaggtga tgggcaaaga gttgggcagc tgaacatcga ggatgacgtc gctggttttt
     2221 ttgtaataat agtcgaaatt cagcgtaaca cgggaacgga acatggtgag gtcgaaaccg
     2281 atgttggttt gtgcggtagt ttcccattta aggtcaggat tgccataagt gatttgcacg
     2341 gcagcggggc ctgaaagtgg attagtaatt tcgcgacgat aatagttgat caatccataa
     2401 cgagcgtaat taggaatgcc ttcctgattt ccattctttc cccaacctcc acgtattttt
     2461 aaatcatcta tgaaagtaag atttttcata aattcttcgg agctcacgcg ccaaccagcc
     2521 gagaaagaag gcatagttcc ccagtgatga gccaactttg aagaaccatc atgacgaata
     2581 ctcatggtaa ggtaatattt tgaaagataa tcccaggtta cacgtccgaa gaaactggcc
     2641 agagcccatt cttcgcgggt agtccctgcg gtgatattat tggcagccca aagtgtacgt
     2701 acgtttgtat cggccgggaa atcattccct tcaatataac tgtcgtttcc ttggaatttc
     2761 tgaatacttg ctcctgctaa ggccgtaaaa ttatgctggt ctgccgtaaa tttataatca
     2821 agggtatttt cccataacca agtaaggtta ttaaacttgt cggagcggcc tataccattc
     2881 atgttacgac cataattggt acggaaagga tcgagataat aatcccactg atgggtagtg
     2941 atatcggttc ctacatgagt tttgaagtaa aggtctttga tgagtcgagc ttccaagcct
     3001 atatttccga atacctgata atcggtggat agttgatcgg ggccgtacat atatgctgca
     3061 ggattttccc atgaaggttg aaatggattg ggatcgtact gcccacttcc atcctgcttg
     3121 tatacatgaa gaaagggtgg agtgttcaat gcactcatga ttacgccccc tcttcctgaa
     3181 ctcaggttgt cgggtgtgtc ttttgtatct atccctaata cgttgatatt agaagtaagt
     3241 tttaaccacg atttcatttc gctgtcgagg ttgattctta cattgtaacg atcaaatcgt
     3301 gctggtttta caatgccttt gtcgctcaaa tagcctccag ccacatagta tttcgactta
     3361 gcacttccgc ccgaaacaga aaattggtag ctttgggtgt agccggtgcc gaaaacatag
     3421 tcgggccaat tattgaaata ggtcatcgaa ggatcgagcc ctccgggaag aatttcttcc
     3481 attaaatctc tatattgttt ggtgttcaat acttctatat tttttctcag gttcgatatc
     3541 ccgaaatagg tattgaaagt taccagaggt tctccttcct gcccgcgttt agtagtaatt
     3601 agcaccacgc cgttagctgc acgtgcacca taaatagccg atgaagaggc atctttcagt
     3661 atggtcatac tggagatgtc gttagggttt aaaccgcgaa tatcggttgt cggaacgcca
     3721 tctactacat ataaagggtc attgccagcc agaacagagg ttgctccacg aactcttact
     3781 gccagctgct gaccaggttt gccagatggt tgtattactt gtactccagc agctttaccc
     3841 tgcaaggcct gagccgccgt aataatagga cggttgcgaa tgtccttttc attaactact
     3901 gctacagcgg tagttaaatc ttttttctgt tgagcaccat aacctattac tactatctca
     3961 ttcaatatag tagtctcctc gctaagcaca acattaatta ccatttgtga tcctactgga
     4021 atctcctgct tgttgtatcc aacaaaacta aaaatcaata tatccttgtc ttcaacttca
     4081 atcttataat ttccattaag atcggtaaca gttccagtgt aagtaccttt tacttgaata
     4141 gtaaccccag ggatagaagc acctttttcg tcggttactt tacctgtaac catttttttt
     4201 tgtccccaga cgctacccga aaatgcaata agagttatta atagcagggt aaaatgtttc
     4261 atattttaat tgattataat tttttgaaaa tcaaaaaacc aaagaaaatt tgagagctta
     4321 ataggaataa gaaagaatct ggggtcaatt gttgacccca gacttgaacc agatgcaaaa
     4381 accctatcgc tcaagaatta ccgaccaagc tgaagaaccg ctgtggtctg cgctgatatc
     4441 aacggagacg aagaggtatt cttttgttcc agtattggca tatcccatca cttcatatct
     4501 attggtaagg ttaccgccat tgggaggatt agctccatcg gaattttcac cgccataata
     4561 acctttgtag aagccgagga aagctgctcc tgtggggcca ttggtaagga tgatcaaagg
     4621 acggctaccc ccagaggctg gaacgaattc ccatgaatgt gttccagaac cgaaggcagc
     4681 tccatttccg ctggctgcaa tttgttcgtc agcccagcat ccattgggcg aactcatata
     4741 accgtcattg cgagcactac catttgtgtt gtaaatatat actcctccat cagcaaatgt
     4801 gaactcatca tcggtgatgc atgaccaatc atcagtacca gaagtgcttc catcgaggaa
     4861 gtttttggga acgctccacc atgaataatc gcccaaacca gggccaacga atatcgaaag
     4921 attttcggct cttggtcgcc atgcgccacc cattaaaagt tcggcagtca attctgtacc
     4981 tgtaacaaaa gcctgtttgg ttactgtact ctcaccattg gggttgcttg cggtaagggt
     5041 aatcatataa attccatcac tggcataagt atgcacggga tctttctcgg ttgaggtatt
     5101 gccatcccca aaatcccata aataagtttc accgttttcg gtagtattgg tgaaagttac
     5161 agtacgacca tccatctgca tgctaaaaga ggcttcaggt tttggccctg gaataggagg
     5221 ttcatcggca ggattgtcgt aatgaaccag tacaaatctc caataaccac ccgaagaagc
     5281 cggatcccaa cgatagatac cttctacaat caatgtatca acagtgccat cgactaattt
     5341 tacaatatta tattcaacat aatcctgggg tgttttaact tcagtgccat tacccaactt
     5401 gtagaaacca atgtaagctc ctttaccaat agctttaatc ttggatggat tgcctacggt
     5461 tagtttgaaa gtgtgatttc catctcccca tgctgataaa tcttcaccat taggaccaac
     5521 catctgatcg gtagtattac actgattttc aggagagaat aatccacctt cagcccaata
     5581 atcgccttgg gcgcggaatt ccattcggcc gtcacgatag aatatccatt catcgttcaa
     5641 ctggcaagga cgattggcaa gctcattatt gtctcttccc atcgcccacc aaatttgaga
     5701 atgatcttct ggaccacatt ccagaggata acgacctgtg gttacatcac gtattaattt
     5761 ccatgtcttt gattccatgc ccgttaaagc agtcaaaaga acattggggt cagacaggac
     5821 aattttggtt gaatacacat cctgattccc attagcatct tttgccgtaa gtgtaaccgt
     5881 gtattctccc tctgcagcat agatgtgaac tggatttact tcggaactgg tttgcccatc
     5941 accaaaattc cagttacatg tggtgtagtt ttgcgaagtg ttagtaaaac ttaccttgag
     6001 aaaattactg gaatcgacct gataggtgaa gccagcaatc actaagtttt catcctcgtc
     6061 gtctttgcag gacgtgaggc aggtaagact gagcagtgcc attacggcaa ggctcatggc
     6121 cgtaaatttc cgaatcgttt tcatgcatgt ttggttttaa ggtttaacat attggacttc
     6181 tgggttaagt gttaaattta agattaatca ttgtatctgt ttgagtgtaa aaaatggatt
     6241 attgtaaaat tcgccctata attaatatct atgttaaaat attgaataat aaaattatat
     6301 actcaaaaaa caatctctat atttttatta gtgtatctga tacaaagata taaaaggttt
     6361 tcaataaaca aatcttttac gaaaaaaaaa ttgaaaattt attttccttt caggttatgg
     6421 tagtgtaatc attgagttaa taaataatgc caaggtatta tgaatcattc aattcctgta
     6481 ttctgatgat ggaacgatga atgcattgtc ctcaaatttc agaactcctg atcctataat
     6541 tgcatatcaa tattttacaa aagtaccac
//
LOCUS       DAOORU010000134         6532 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_14407_length_6532_cov_4.341825, whole genome shotgun sequence.
ACCESSION   DAOORU010000134 DAOORU010000000
VERSION     DAOORU010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14407_length_6532_cov_4.341825"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2780)
                     /gene="porU"
                     /locus_tag="PLF20_08250"
     CDS             complement(<1..2780)
                     /gene="porU"
                     /locus_tag="PLF20_08250"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOX81029.1"
                     /translation="MTYKNITRLFFVFALVFHVFSVSAQRRILHWETLQTTTDTTLNL
                     WLQEAAYINDSLNVPVYLEEIPLPVGSNGLEYNLKEVYFEKLESKISGIGTEIKINVT
                     IVTECKQKKARITFIPLRINPENGQSEKLVSFEPSINFTTDKEVQFPASDFASSSLLA
                     SGKWYKIGVAQTGIYRITYHDLKKWGVNPDQIDPRTISLHGFGGRLLPESNLQKTQDD
                     LPPVAIEVIGEEDGIFNPDDYILFYGENPHKWTLNVLGQKLTHQTNIYEDTTYYYLTY
                     GIQNPARIEDFIPPTLSITHKIDYFTYPLLHEKNEQHLTKSGKEWYGETFNENSRQNF
                     TFIVPDHKVGEKGIIELEVAGRSYEANSFVLSYNEQDLATIPLAGISGNLMSQLYARK
                     GKALLNFTPQSDTIILDLTHVKNHPYSIGYLKYIEVNVIRQLIFRGPQMLFNYLPRPQ
                     TGQIPEISVQTTQPDLKIWLVDNLFQPQNIPLQNTDTSVYFKIQSYNGHYFIAFDGSS
                     YYSPSFIEEVPNQNLHALQPAEFIIVSPKVLMNQAKRLAQIHQNREGIGTLTVDVKEI
                     YNEFSSGAKDPSAIRNFMRMLYERAQPGEEPHYLLLFGDGSYDPKHRIPGNKWHIPTF
                     QSQESLEMSSSFVCDDFYGLLDPEEGLDAYGAIDVGVGRFPVYTIEEAKNMVDKIENY
                     FKEGPPAMLPWKNTFCFIADDENGNEHVKGAEKLANIVDTSSSIINVNKIYIDAYKQY
                     STSNGDKYPDVNKAITDQVNEGALFINYTGHGGETGWADENILTVPEIFNWNNKGKMP
                     IFITATCQFSRYDDPERVSAGEHVIQYNNKGGVALFTTTRLTFSHTNNFLNQAFYVSL
                     FEQLKSGNIPHMGDLIRMSKNKNNNNISIRNFSLLGDPVLIPAFPRYSIKTTHINDIP
                     IEYFIDTLRGCQ"
     gene            complement(2809..>6532)
                     /gene="porU"
                     /locus_tag="PLF20_08255"
     CDS             complement(2809..>6532)
                     /gene="porU"
                     /locus_tag="PLF20_08255"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOX81030.1"
                     /translation="DNYSLKDTLSGNQRTILYFHDATNLYETGGLPIFIQRIPLKQLS
                     DSIFVKIFPLISGEDNSTDVVSSEFLNNEYQFFIKKLEINGKAYGQVGILPVRIHPIH
                     NKIEVLEKFHLEITVKEQAFQNFPQLKMGTIQSVLSQGEWYKIQIAETGIYKISFETL
                     QSQGINLSGIDPRNIHIHGNGGRVLPEANNMTRYDDLMEMSIYIFGEEDGKFDPGDYI
                     LFYAQGPGSWAFNTQTKRFQFDKHPYSDFAYCYITINNSKGKRIQLNPLPEVEATQTI
                     TTFTAYIHHEEDLINLGKSGRQWYGESFDIQNTMNFAISLEGVVNGSRVLLESAVVAS
                     SYSISNMKISVGETPFLVQTISPIPPPSLDSDYGKRSIDTASFKANSTTVSLSYKYIK
                     PLNSSQAWLDYFTINYQRNLSFSGSQMSFRKPMHYSSTQVVRYKIGNASQNIQIWEVT
                     NALRPMRMQTQLAGNTLTFDSKNDTLREFIAFDGNKFLNPTFIGKVPNQNLHGMIDAD
                     YIIISHPDFLSQAQELANIHESENQFNVKIVTPEMVYNEFSSGIQDPSAIRDFMRHLR
                     LSSGNIRKPAYLLLFGDASYDYKDRIQGNTNFVPTYQSDESLNPSYSFATDDYFTFLD
                     QSEGKGGGGTPDLCVGRIPVFTQQQADDYMKKFKQYISVSTTSMGEWRTNLCFVADDE
                     DNNSHLRQADGLANFIDTTYPHFNIDKIFLDAYDQITTPGGQRYPDVNKDITQRIENG
                     LLIINYTGHGGELGWAHERILEISDINSWNNFYKLPLFITATCEFSRYDDPQRVSAGE
                     YVFLNPKGGGIALFTTSRLTYGGSNITLNQNFYKYAFERTNGQYRTLGEIIMIAKQKS
                     GSDLNWIKFILLGDPALKLAYPENKVSALTINNTSIEISTDTLKALSNVSITGEVTDP
                     LSYPLHKFNGTVTTTIFDKVSLIKTKANDPESKPTTFALRKNIIYKGLSEVKNGKFYM
                     NFIVPKDIEYKIGEGKICFYACSDKDDAAGYFAPVYVGGINTNAQIDNTPPSIHLFMN
                     DLNFKDGGITDENSVLLAFISDENGINTVGSGIGHDIVAILDNNSNNPLILNNYYKSD
                     LNTYTSGSIAYPLFNLEEGDHEIRLTAWDVYNNSATSKLKFTVKNSASFIISNLYNYP
                     NPFYDKTYFVFEHNKSSEEMQVEIRIMDIYGRVVKTIAHTIFFTGYCSQPIEWDGASD
                     NGKRLSQGIYIYQLTLTDSKGNARQKSGKLMISKNQ"
BASE COUNT         1854 a         1210 c         1387 g         2081 t
ORIGIN      
        1 ttttggcaac ctctcaaggt atcgatgaaa tattcgatgg gaatatcatt gatatgtgtc
       61 gttttgatag aatatcgagg aaaggccggt ataagtacag ggtctcccaa taaactgaaa
      121 tttcggatcg atatattgtt gttgtttttg ttcttcgaca tccggatgag gtctcccata
      181 tgaggtatat tgcctgactt gagctgttca aataacgaga catagaaggc ctgattcaag
      241 aaattgtttg tatgtgaaaa tgttagacgt gtagttgtaa acaaggcgac gccacctttg
      301 ttgttgtatt gtatgacatg ttcgccagcc gaaacccgct ccggatcatc atagcgacta
      361 aactggcaag tagcagtaat aaagatgggc attttcccct tgttgttcca attaaaaatt
      421 tcgggcactg taagaatatt ttcatctgcc catccggttt ctcctccatg accggtatag
      481 ttgataaaca gagctccttc gttcacctga tcggtaatag ccttgttgac atcgggatat
      541 ttgtcgccat tagaggtgct atactgtttg taagcatcta tataaatttt gttaacgtta
      601 attatcgaag aagaagtatc tacgatatta gcaagctttt ctgctccctt tacatgttcg
      661 tttccattct catcgtcggc tataaagcag aacgtgttct tccatgggag catggcggga
      721 ggaccttctt tgaagtaatt ttcaatctta tctaccatat tttttgcttc ttcgatagta
      781 tatacaggga atcgcccaac acccacatct atcgctccat aagcatctag tccttcttca
      841 gggtctaaaa ggccataaaa atcgtcacat acgaaagaag atgacatctc taaactttcc
      901 tgagattgaa aggtagggat atgccatttg tttccaggta ttctatgttt gggatcgtag
      961 cttccatcgc cgaaaagaag aaggtaatgg ggttcttcgc cgggttgtgc gcgttcatac
     1021 agcattcgca tgaagttacg aatagctgaa ggatcctttg cacccgacga aaattcattg
     1081 taaatctctt tcacatctac ggttaaagta cctatccctt ctctattttg atgaatttgt
     1141 gccagacgtt tcgcctggtt catcaatact ttcggactca caatgatgaa ttcggcaggc
     1201 tgtaaagcat gaaggttctg attgggcact tcttcgataa aggagggaga atagtatgaa
     1261 ctaccatcaa aagctatgaa atagtgtcca ttgtaagatt gtatcttaaa atagaccgaa
     1321 gtgtcggtat tttgcaatgg gatgttctga ggctgaaaca aattgtcgac caaccagatt
     1381 tttaaatcag gttgagtagt ttgaaccgaa atttcgggaa tttgaccggt ctggggtctt
     1441 ggaagatagt tgaaaagcat ttgaggccct ctaaaaatca attgtcgtat cacatttact
     1501 tcgatgtatt taagatagcc gatgctgtaa ggatggttct ttacatgagt caagtcgaga
     1561 ataatcgtat ccgattgagg agtaaaattc aatagtgctt tccccttgcg ggcgtataat
     1621 tgactcatca aattgccact aataccagcc aaagggatag tcgctagatc ctgctcatta
     1681 tatgacaata caaatgagtt tgcttcataa gaacgtccgg caacctccaa ttcgataatt
     1741 cctttttcac cgaccttatg atcgggaact ataaaagtaa aattctggcg gctgttttcg
     1801 ttgaaggttt cgccatacca ttctttcccg cttttagtga ggtgttgttc gtttttctcg
     1861 tgtaaaagtg gataagtaaa ataatctatc ttatgggtta tagaaagcgt aggaggaatg
     1921 aaatcctcga tgcgtgcagg gttttgtatt ccataggtta aataataata ggtagtatct
     1981 tcatatatat tggtttgatg agtaagtttc tgccccaaaa cattgagtgt ccatttatgc
     2041 gggttttccc catagaaaag gatataatcg tcggggttga aaataccatc ttcttcgcct
     2101 ataacttcga tggctactgg aggtaaatca tcctgtgttt tttgtagatt cgattcggga
     2161 agtaatcttc caccaaatcc atggagtgaa atggttcgag gatctatttg atcgggattc
     2221 actccccact tttttaagtc atgataagtg attctataga ttcctgtttg ggcaactcct
     2281 attttatacc atttccctga ggcgagtaat gagctgcttg cgaaatctga agcagggaat
     2341 tggacttcct tatctgtcgt aaaattaatg gaaggctcga acgaaacaag tttttcggat
     2401 tgtccatttt caggattaat cctcaatgga ataaaagtta tccttgcttt tttctgcttg
     2461 cattcggtaa cgatagttac attgattttt atctcggttc ctatcccact aattttcgat
     2521 tcgagttttt cgaaatacac ttccttcaga ttatattcga ggccgttgct cccaactgga
     2581 agaggtattt cttccagata aacgggaaca ttcaatgagt catttatata ggcagcctct
     2641 tgcaaccata aattcaaggt agtgtcagta gttgtttgta atgtttccca atgtagaatc
     2701 cttcgttgag ctgagacgga gaaaacatga aaaacaagag caaagacgaa gaacaggcga
     2761 gttatgtttt tataagtcat cgaaaatatg gtgtattatg agcaaagttt attgattttt
     2821 cgaaatcatg agtttgccgc ttttttgtcg agcgtttcct ttcgagtcgg ttaacgtcaa
     2881 ttgataaata taaattccct ggcttagtct ttttccgttg tccgaggcac catcccactc
     2941 gatgggttgg ctacagtatc ctgtaaagaa aatagtatga gcaatagttt tgacaacccg
     3001 gccataaata tccattatcc ttatttcgac ctgcatttct tctgagctct tattatgctc
     3061 aaaaacaaaa taagttttat cataaaaagg gttaggataa ttatatagat tcgaaataat
     3121 gaaggaagca gaatttttca cggtgaattt cagtttggat gtggccgaat tgttataaac
     3181 gtcccatgcc gtgaggcgta tttcgtgatc cccttcttcg agattaaaca agggataagc
     3241 aatacttcca ctggtgtagg tatttagatc tgatttgtaa taattattca ggatgagggg
     3301 attgttcgaa ttgttgtcga ggatggccac aatatcgtgg ccaatgccgc tacctacggt
     3361 atttattccg ttttcatcgg aaataaaggc gagcaggaca gagttttcat cggtaattcc
     3421 tccatctttg aaattcagat cattcataaa taaatgaata gagggaggtg tattgtcgat
     3481 ttgggcgttg gtattgattc caccaacata aacaggtgcg aagtaaccgg ccgcatcatc
     3541 cttgtcgctg catgcataaa agcaaatttt gccttcgcca attttgtatt caatgtcttt
     3601 tggtacgata aaattcatgt aaaacttacc atttttaact tccgataaac ctttataaat
     3661 gatattcttt ctcaatgcaa aggtagtagg tttactttcc gggtcatttg ccttggtttt
     3721 gatgagcgag accttatcga aaatggtggt cgtaacggtt ccattgaatt tatgtaatgg
     3781 atagcttaga ggatcggtaa cttcgccagt tatggaaaca ttcgacagag ccttgagggt
     3841 gtcggtagaa atttcgatcg aagtattgtt gatagtaagt gcactaactt tattctcggg
     3901 ataggcaagt tttagtgctg gatcaccgag caaaatgaat tttatccaat tcaggtcgga
     3961 accgcttttt tgcttggcaa tcatgataat ctcgcccaag gtacggtatt ggccattcgt
     4021 tcgttcgaat gcatatttgt aaaaattttg gttaagagtt atattagaac caccgtaggt
     4081 caatcgtgat gtagtaaata atgcaatgcc tcctcccttt ggatttagga aaacatattc
     4141 tcctgccgaa acgcgttggg gatcgtcata gcggctgaat tcgcaggtgg cagtaataaa
     4201 cagaggtagt ttatagaagt tattccatga atttatatca ctaatttcga ggattcgctc
     4261 atgagcccag cccagctcac caccatggcc cgtgtaattg atgatgagga gtccgttttc
     4321 gatgcgctgt gtgatatctt tatttacatc cggatatcgt tggccaccgg gtgtagtaat
     4381 ttgatcgtaa gcatcgagga aaatcttgtc aatgttgaaa tgaggatatg tagtgtcgat
     4441 aaaattggca aggccatcgg cttggcgtaa atgcgaatta ttgtcttcat cgtcggcaac
     4501 aaaacaaaga ttggtgcgcc attctcccat tgaagtcgtg ctcacactga tatattgttt
     4561 gaattttttc atataatcat cggcttgttg ctgggtgaaa acgggtattc ggccaacaca
     4621 tagatcggga gttccacctc cacctttccc ttcgctttgg tcgagaaagg taaaataatc
     4681 atctgtagca aaagaataag aaggatttaa ggattcgtcc gattggtaag tgggtacaaa
     4741 gttggtattt ccctgaatgc ggtctttata atcgtaagag gcgtctccaa atagtagaag
     4801 atatgctggt ttacgaatgt tgcccgaact caacctcaaa tgcctcataa aatcacgaat
     4861 ggcgcttgga tcttgtatgc ccgacgaaaa ttcattgtaa accatctcgg gagttacaat
     4921 ttttacattg aattgatttt ctgattcatg gatattagca agttcctgag cttgtgacaa
     4981 gaaatcggga tggcttatga tgatataatc tgcatcgatc atcccatgaa gattttgatt
     5041 tggaacttta ccaataaaag tcgggtttag aaatttgttt ccgtcaaaag cgataaattc
     5101 acgcaaggta tcgtttttcg aatcgaaagt aagcgtattt cccgcaagct gggtttgcat
     5161 cctcataggc cgtaaggcat tggtcacctc ccatatctga atgttctggc tggcatttcc
     5221 tatcttatat cgtacaactt gggtactcga ataatgcatg ggtttacgaa agctcatctg
     5281 tgaacccgaa aaactcagat tacgttgata gttaatggta aaataatcga gccaggcctg
     5341 agaagaattc aaaggcttga tatatttata ggaaagtgaa accgttgtac tattggcttt
     5401 aaatgaagct gtgtctatgc ttcttttccc ataatctgaa tcgagagaag ggggaggaat
     5461 aggagaaata gtttgaacaa ggaaaggagt ttctcccact gaaatcttca tattgctgat
     5521 agaataagaa cttgcgacca cagccgattc gagtaaaaca cggcttccgt taaccactcc
     5581 ctcgagggaa atcgcgaaat tcatcgtgtt ttgtatatcg aaggattcac cataccattg
     5641 ccttccactt ttaccaagat tgatcaagtc ttcttcatga tgaatataag ccgtaaaagt
     5701 ggttatggtt tgagtggctt cgacctctgg aagtggattt aattgtattc ttttcccttt
     5761 agaattgttg atggtaatat aacaataagc gaaatcggaa taaggatgtt tatcgaactg
     5821 aaaacgtttt gtttgggtat tgaaagccca cgaaccaggg ccttgggcat aaaaaagaat
     5881 ataatcaccg ggatcgaatt tcccgtcttc ttcgccaaaa atataaatac tcatttccat
     5941 caaatcatca taacgagtca tattattggc ttcgggaagc accctgccgc cattgccatg
     6001 gatgtgaatg tttcgtgggt cgattccact tagattgatt ccctgcgact gaagtgtttc
     6061 aaaggatatt ttgtaaatgc ctgtttcggc aatctgaatc ttgtaccatt cgccctggct
     6121 caaaaccgat tgaatagtac ccattttcaa ctggggaaaa ttttgaaaag cctgttcttt
     6181 cacagtgatt tctaaatgaa acttttcgag aacctcgatt ttgttatgga taggatgtat
     6241 gcgtactggt aatattccaa cctgaccata ggcctttcca ttgatttcta atttttttat
     6301 aaagaattga tattcattgt ttagaaactc tgacgagacg acatcagtgg agttgtcttc
     6361 tcccgaaatt aaagggaata tcttcacgaa aatgctgtcg gataattgct tcaaaggaat
     6421 cctttgaatg aatatgggga ggcctccggt ttcgtaaagg ttagtagcat catgaaaata
     6481 caggatggtt ctttgatttc ccgaaagagt atctttcaga ctataattat cc
//
LOCUS       DAOORU010000135         6465 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_14599_length_6465_cov_5.374883, whole genome shotgun sequence.
ACCESSION   DAOORU010000135 DAOORU010000000
VERSION     DAOORU010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6465
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14599_length_6465_cov_5.374883"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="PLF20_08260"
     CDS             <1..364
                     /locus_tag="PLF20_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81031.1"
                     /translation="QLAVYKEVTTLRRSSWVKTGLEEMSPINVRKENRTRGHVFICML
                     AYKLLFHIWKYFEEDLSTSDKFGSRSTQQYILDCLDEINYIEYQFENTTIKILPKIFN
                     DDQQYILDKLKIKLPQIL"
     gene            complement(989..3631)
                     /locus_tag="PLF20_08265"
     CDS             complement(989..3631)
                     /locus_tag="PLF20_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81032.1"
                     /translation="MRRLIIFLFCLTTFLPLFSQVIIEGKVKDVQNKPLSDIHVMVSM
                     LHSDNIVGFDITTDEGQFRINLRCVGDSVRIKTSSVSHEDFKATIECKSKHLDIILKE
                     SVKDLKEITIKAKHVIKKGDTLSYLVKPFAREQDRTINEVIRRIPGIEVEPSGKILYE
                     GTPISNLYVEGMDLMEDRYSLLTDNLPYDKVSTVEVITNHEPVKILQKYSTSGKVALN
                     LRLRNKTTLTGNAELAGGIPYFTHKVNLTPILFQPTLQSLFSFQSNNSGEPFVETFGP
                     MLTYQPNLRFDDPVSIPSPSPRNIPVADLPPKSYIDNSSLLTSAYILTRSTNQWEWRT
                     SISYKNDKIDKNWSGRTSYLLPTDTLMFDETSAFTSKSKIWTAEINLRHNTEKYYADN
                     RLSFSLEPAKQSGIFTRNSFAYKESFTEKPQKLDWNGRWIFPTRKKLIEVYSFIDYHY
                     NPDLSLRTFPGYIHLNQTSDSVIQYFSGNRLACENYVSLYSSLKSFSLRMDAGFSYQR
                     LHIESNLHSYEANFDFTGQDTNYWNDNVQKRFRIYFRPTLMKRTATTTWNFQIPLSSL
                     LLTEESNIHMVSQNYAKIVAEPSLLYSKKIKNNWNVTASAKRYYQYFASSEIFYGYIL
                     ASPDLLKKNEPLVSGRLYAVNHANATIKYNNIVTGFSAYGSVGYSLQQFDFLRSDSIG
                     LNGSRIYTIKNYANNRQLASLRGSISKSLPDVRTNFAINLNLQSDKGKVLVNGELNDS
                     KTHYITLNPTIYHYFSVFDLVYAFRWLNIDTELGNSSQKQNSDWFRHELSITGNFSRK
                     NLIVTASFIYNDLNKNRIFLTDLSIRYKIKKILSSKMETGLLINNLFDAGKYTYNQLG
                     SYVNLVTEFPLRPFQCLLFVRFNY"
     gene            complement(3656..4486)
                     /locus_tag="PLF20_08270"
     CDS             complement(3656..4486)
                     /locus_tag="PLF20_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLPGLI family protein"
                     /protein_id="HOX81033.1"
                     /translation="MNYNKLKLFFFILLGSSSYLGFSQIFPIPKDIFVEEPKLKVIYR
                     YHYQLDSTNPDEIRKRTIWLLIGDNYSSFEDPKKKWYKDEMSKINSQQQLQDWIYKNT
                     AKMVHENFTIIKNRKLKRLTYYDYILMSGHYRYEETPHLIWKLESDTANILGYTTQHA
                     WTYYGGRIWHAWFTSAIPISDGPYKFMGLPGLILKVEDSKQHYIFEAIAIEQPAEGEN
                     ITYEENQYIETTREKFLEANRNLRLDIINRAQEAGLDAGAQQTAARNMRSHNNPIELK
                     "
     gene            complement(4588..4824)
                     /locus_tag="PLF20_08275"
     CDS             complement(4588..4824)
                     /locus_tag="PLF20_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81034.1"
                     /translation="MELKNLKDSWIPCKIYLSLPSSVGMGQPNKINAAPEEDACRTII
                     VVCPDDGTIAGYAIICDMTDLEVWIAELCGANPN"
     gene            4938..>6465
                     /locus_tag="PLF20_08280"
     CDS             4938..>6465
                     /locus_tag="PLF20_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOX81035.1"
                     /translation="MLKKYTFFMMLIILTSIANAQGFSTVGKLKKVNYTSSFIELFTT
                     NGQVRITPYSDNIFRIRIVKDVFGPDVAYAVIAQPEAVKYSRNEDAENIILKTSTTIL
                     KIGKNPVRFTFLTLDGRIINGDDPALGTNWNGTEVTTCKILQAGEHFIGLGEKTGNFD
                     RRGNAYWHWNTDNPRYDEYSDPLYSTIPFYIGLHSGLTYGIFFDNTYKSYFNFGASQE
                     RFSSFGADDGEMDYYFINGENVAEIIKNYTWLTGRMELPPIWALGNQQCRWSYYPDTE
                     VLEIASMFRDKKIPADNIYLDIHYMDAYKIFTWHPQRFPHPDQLVNNLKKMGFHTTAI
                     IDPGIKVEKGYTAYEDGLKQHMFIKYPDGEPYQGQVWPGWCHFTDYTKPVARQWWGKQ
                     FASLVELGIDGFWNDMNEIATWGSATPTFIQFDWEGYKTTYRQAKNIYGMLMARSTYE
                     GTKELMHGGRPFVLTRSGYAGMQRYTSIWTGDNQANDNHMLLGIRLVNSIGLSGVAFT
                     G"
BASE COUNT         1997 a         1185 c         1274 g         2009 t
ORIGIN      
        1 tcaacttgct gtatacaagg aagttacaac cctaaggcgc tcaagttggg ttaagacagg
       61 tctggaagag atgagtccta ttaatgtcag aaaagaaaat agaacccgag gacatgtgtt
      121 tatatgcatg ttggcttata aacttttatt ccatatctgg aaatactttg aagaagattt
      181 gtcaacttcc gataaatttg gtagcagatc tactcagcaa tatattttgg attgcttgga
      241 cgagataaat tacatcgaat atcaattcga gaatacaacc ataaaaatat tacccaaaat
      301 ttttaatgat gatcaacaat acattttaga caaattaaag ataaagttgc cacaaatatt
      361 ataagcaatg gattttctca ttaaaattct tttaaataat taacatacaa attatttaaa
      421 gctaaatata gaaggaatat ataggaaaat ttgatgaaaa attgaaaaat tcagtactcc
      481 agattctata attgcatatc aatattttaa aaaataatat cctattctaa attgtcgtat
      541 tttctggcaa aaattttcta atatgtaaac ctaacagatc ttaaaagcac tcagaaaaat
      601 cgattcaatc ctcggtgtct ttatgcctat gcgcgaattc ttttctcgcc aagatgccaa
      661 gaacgctaag aaaaatcatt tcaaatcctt agctccgctt gcaacccatt tctcgttaac
      721 aagctaagaa ccattaaaat tatcacgtca ggaaaagaaa tttaaaaaaa gaattaatgg
      781 tggtagatga tggaagtaat cctcaagagt atgataagag tggtgattaa aggaggtaat
      841 gctgtcacgc aatcatcaaa accacgataa gctgattgaa accgaatcag gtgttatgaa
      901 atttattaca ctatcattca tcgaaagaca gtgcaagatg aggatggtcc tttgtaataa
      961 ttttttatga aatcattact cttatttatt aataattgaa cctgacaaat aacaaacatt
     1021 gaaatggtcg taatgggaac tcggttacca gattaacata actgccaagc tgattgtaag
     1081 tgtatttccc tgcatcgaac aaattgttga tcaataagcc tgtttccatt ttcgatgata
     1141 aaatcttctt tattttatag cgaatactta aatcagtaag aaaaatacgg tttttattca
     1201 aatcattata aataaatgaa gccgttacaa ttaaattttt tctactaaaa tttccggtaa
     1261 tggaaagctc atgacgaaac caatcgctat tttgcttctg actagaattg ccgagttcgg
     1321 tgtcaatgtt caaccaacgg aaagcataaa ccagatcgaa taccgaaaaa taatgataaa
     1381 tagttgggtt tagggtgatg taatgtgttt tgctgtcatt taactctcca ttgactaaga
     1441 cctttccttt gtcggattgc aaattcaaat tgatggcgaa attggttctt acatcgggta
     1501 aggatttgga gatagaacct ctgagcgaag caagttgacg gttgtttgca taatttttta
     1561 tagtgtaaat acggctgcca ttgaggccaa tagaatcaga ccttaaaaaa tcgaactgct
     1621 ggagagagta accaacactg ccataagccg aaaaacccgt tacaatattg ttatatttta
     1681 tggtggcatt ggcgtggttt actgcatata atctaccgct aacgaggggt tcgttcttct
     1741 tcagcaaatc ggggcttgcc aaaatataac cataaaatat ttcggaagaa gcaaaatatt
     1801 gataatatct ttttgcagaa gccgttacat tccaattatt ttttattttt ttgctgtaaa
     1861 gcagagaggg ttcggcaact attttggcat aattctgaga gaccatatgt atgttcgact
     1921 cttccgtcaa taaaagcgaa cttaagggta tttgaaaatt ccatgtcgta gtagccgtcc
     1981 ttttcatcaa ggttggcctg aaatatatcc tgaacctttt ctgaacattg tcattccaat
     2041 agtttgtatc ctgccccgta aaatcgaagt tagcttcata agaatgcaaa ttggattcta
     2101 tatgtaggcg ttgatatgaa aatcctgcgt ccatccttaa gctgaatgat ttcaagctgg
     2161 aatacagtga aacataattc tcacaggcaa gcctgtttcc cgaaaaatac tgaatgacag
     2221 aatcggaggt ttggtttaaa tgaatgtatc cgggaaatgt tcgtagtgat aggtcgggat
     2281 tgtaatgata gtcaatgaaa gaatagactt ctatgagttt ttttcgggta gggaaaatcc
     2341 agcgaccatt ccagtccaat ttttgtggtt tttcagtaaa tgattcttta taggcaaaag
     2401 aatttcttgt aaatattccg ctttgcttgg ctggctcgag tgaaaaactc aatctgttgt
     2461 cggcataata tttctcggtg ttgtgtctta gattaatttc tgccgtccat attttactct
     2521 tcgaagtaaa agccgacgtt tcgtcgaaca ttagcgtatc agtaggcaat aaataagatg
     2581 ttcgccccga ccagttttta tctatcttat cgtttttata cgaaattgaa gttcgccatt
     2641 cccattgatt tgtggaacgc gtaagtatat aagccgatgt caagagggag gaattatcga
     2701 tataagattt gggaggtagg tcggctacag gaatatttcg tggcgaaggt gaaggtatgg
     2761 aaacgggatc gtcgaagcga agatttggct ggtaagtgag catggggcca aatgtctcga
     2821 caaagggttc gccactgttg ttcgattgaa agctaaaaag tgattgtaac gttggttgaa
     2881 ataaaatagg tgtcagattg actttatgcg taaaatatgg aatgccacca gccagctcgg
     2941 cattgcctgt gagggttgtt ttattcctta atctcagatt gagagcaact ttccccgacg
     3001 ttgaatattt ctgaagaatt tttactggct catgattagt aatcacctca acggtagaaa
     3061 ccttatcata aggcagattg tccgttagaa gagaataacg gtcttccatc aaatccatgc
     3121 cttctacata aagattcgat atgggtgtac cctcgtaaag tatcttaccg gaaggctcta
     3181 cttctattcc gggaatacgg cggattacct cgttaatcgt cctgtcctgt tcgcgtgcaa
     3241 aaggttttac cagataactt aaggtatcgc cttttttaat gacatgcttg gcttttatgg
     3301 taatctcctt gaggtctttc accgactctt tcagaatgat atcaagatgc ttacttttac
     3361 actctattgt agccttaaaa tcttcatgac tgaccgaaga ggtttttata cgtacagaat
     3421 ctcccacaca acgcagattg atacgaaatt gtccctcatc agtggtaata tcgaaaccaa
     3481 cgatattatc ggaatgtaac atagaaacca ttacatgaat atcgctcaag ggtttatttt
     3541 gcacgtcctt gactttccct tcgattatta cctgcgaaaa aagaggtaaa aaagtcgtta
     3601 gacaaaataa gaatataata agcctgcgca ttttcataat gtggaggaag gcttattatt
     3661 taagctctat tggattgtta tgcgaacgca tatttcgagc tgcagtttgc tgtgctccgg
     3721 catccaaacc agcttcctgt gccctgttga tgatgtcgag acgtaaattg cgattggctt
     3781 ccagaaattt ctctctggtg gtttctatgt attgattctc ttcgtatgta atattttctc
     3841 cctcggctgg ctgttctatg gcaatagcct cgaatatata atgctgtttc gaatcctcga
     3901 cctttagaat gaggccgggt aaacccatga atttataggg accatcgctg atgggaatgg
     3961 cagacgtaaa ccatgcatgc caaatgcgac cgccataata tgtccaggca tgctgtgtgg
     4021 tatatccaag aatattggcc gtatccgact cgagtttcca gattagatgt ggagtttctt
     4081 catacctgta atgcccgctc atcaaaatat aatcgtagta agtaagtctc tttaattttc
     4141 tgtttttaat gatagtaaaa ttttcatgaa ccatctttgc tgtattttta tatatccagt
     4201 cctgcaattg ttgttgggag tttattttac tcatttcatc cttataccac ttttttttag
     4261 gatcttcgaa tgaggagtaa ttatcgccta tcaataacca tatcgttctt ttcctaatct
     4321 cgtctggatt ggtagaatca agctggtaat gatagcgata aatgactttg agctttggtt
     4381 cttcgacaaa gatatctttt gggatgggaa agatttgcga aaagcccaga taagaagagc
     4441 tacccaacag aatgaagaaa aagagtttta atttgttgta attcattttg ttaatattga
     4501 agttagaaaa atagcaaatt tataaactga tacagcagag cctttaatag cttcatgcta
     4561 tcgtaaggct ctgctaaatt aattgaatta atttggatta gctccgcata gctcagctat
     4621 ccaaacttct agatctgtca tatcacaaat aatagcatat cctgctatgg ttccgtcatc
     4681 gggacatact acaattatag tgcgacaggc atcttcctca ggggcagcat ttattttatt
     4741 tggctgtccc attccaacag aggagggcaa actcaggtaa attttacatg gaatccacga
     4801 atcttttaga ttttttagtt ccaatcatac atttcttctt tgttcgcagg gaatcgtatt
     4861 tttgactcca ttcattgaaa gttttcgcaa gtgctgaaaa aatttataag tttgtccttt
     4921 ccgaaaatat aaataccatg ttaaagaagt acacattttt tatgatgctt attattctaa
     4981 cctccattgc caatgcacag ggattcagta cagtgggaaa actcaagaaa gttaactaca
     5041 cctccagttt catcgaatta tttacgacca atggtcaagt tagaataact ccatatagcg
     5101 ataatatctt ccggatacga atcgtaaaag atgtattcgg acctgatgtt gcttatgcgg
     5161 taattgccca acccgaggca gtaaaataca gtcgaaatga agatgccgaa aacatcattc
     5221 tgaaaacttc gactactata ttgaaaatcg gcaagaaccc tgtaagattt acatttctga
     5281 ccctggatgg tcgaatcatc aacggggatg accctgcatt agggacaaac tggaatggca
     5341 ccgaggtaac cacctgtaaa attctgcaag caggggaaca ttttattggt ttaggcgaga
     5401 agacaggaaa cttcgaccgc cgcgggaacg cctattggca ttggaatact gataatcctc
     5461 gttatgacga atattccgat cccttatatt ccactatacc tttttacatt gggctgcatt
     5521 cgggtttgac ctacggtatt ttctttgaca acacctataa atcctatttt aattttggag
     5581 cttcgcaaga aaggttctcc tcttttgggg ccgatgatgg agaaatggat tactatttca
     5641 tcaatggaga aaatgtagcc gaaatcatca agaattatac atggcttacc ggccgtatgg
     5701 agcttcctcc tatctgggcc cttggcaatc agcaatgccg ctggagttac taccccgata
     5761 ccgaagtact cgaaattgca tctatgtttc gtgataaaaa aatccctgcc gacaatatct
     5821 atctcgatat tcactacatg gatgcctata agatatttac atggcatcct cagcgtttcc
     5881 ctcatccgga tcagttggtc aataatttga aaaagatggg atttcatact accgcgatta
     5941 tagacccagg aattaaagta gaaaaaggat atactgctta tgaagacggt ttgaaacagc
     6001 acatgtttat caagtatcca gatggtgagc cttatcaggg gcaggtttgg ccgggatggt
     6061 gccactttac cgattatacc aaaccagtag cccgtcaatg gtggggtaaa cagtttgcca
     6121 gccttgtcga attgggtata gatggattct ggaacgacat gaacgagatt gctacatggg
     6181 gttcggccac cccgactttt atacagtttg attgggaagg atataaaacc acttatcgtc
     6241 aggcaaaaaa catttatgga atgctaatgg cacgcagtac ttacgaagga accaaggaat
     6301 tgatgcatgg gggaaggccc tttgttctca ctcgctcggg ttatgccgga atgcaacgct
     6361 atacctccat ttggacaggc gataaccaag ctaacgacaa tcacatgtta ctcggaatca
     6421 ggttggtaaa cagcattgga ttatctggcg ttgcttttac gggat
//
LOCUS       DAOORU010000136         6446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_14662_length_6446_cov_5.372712, whole genome shotgun sequence.
ACCESSION   DAOORU010000136 DAOORU010000000
VERSION     DAOORU010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14662_length_6446_cov_5.372712"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /gene="malQ"
                     /locus_tag="PLF20_08285"
     CDS             complement(<1..1435)
                     /gene="malQ"
                     /locus_tag="PLF20_08285"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011079.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOX81036.1"
                     /translation="MKIERKSGILLHITSLPGLYGIGTLGKNAFDFVDFLVKAKQKIW
                     QILPLGPTGFGDSPYQSFSTFAGNPLLIDFEKLIEKKWLTPSEFDDAPGNDDYVDFGS
                     VIEFKSKTLRSAFQGFKDVASIDEKIVFSNFCSENQAWLDDYAIFMELKSFHGGRPWH
                     QWDEPYKMRHRIALDEFIVSRQQEIEFWKFLQFVFFEQWSALKAYANQNGIRIIGDIP
                     IFIAHDSADAWSHPEVFQFDEHHNPTFIAGVPPDYFSETGQLWGNPLYNWDYLKQTAF
                     AWWLDRIKAALSLYDFIRIDHFRGFAAYWAVPFGETTAINGKWIDAPGAELFETIQQK
                     LGNLPIIAEDLGVITPDVEELRDRFHFPGMKVLQFAFSPMKNNEYLPHNYNTSNYICY
                     TGTHDNNTTLGWYNELDGATKSGIWRYLSGGNENTVWKFIRLAWSSVAFIAVAPMQDI
                     LELDYTARMNTPGTSSNNWQWRFKKSDY"
     gene            complement(1528..2916)
                     /locus_tag="PLF20_08290"
     CDS             complement(1528..2916)
                     /locus_tag="PLF20_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX81037.1"
                     /translation="MSSLQAKQYSKPKLSFWQIWNMSVGFLGIQFGFALQNANASRIL
                     QTFGADVEHLSWFWIAAPLTGMIVQPIIGHYSDKTWCKLGRRRPYFLAGAILASLALV
                     FMPNAELLTSVIPPMFIGAGILMILDASINVAMEPFRALVADMLPTEQRTLGFSIQTA
                     LIGIGAVIGSWLPYIFAEWLGISKTAPEGQVPDNVVFSFYVGAAVFISCVLWTVIRTK
                     EYPPEQLRQFSDIPADEQTKGGFIEIFRDFAHIPRTMKQLGVVQFFSWFALFSMWVFT
                     TPAVAVHIYGAAIDDRSSEIYQNAGNWVGICFGVYNAISALYAFIIPSIAGKIGRKLT
                     HSLSLILGGIGLISIYFVPSPKWLLLPMVGIGIAWGSILSMPYSILAGSLPAQKMGVY
                     MGIFNFFITIPQIINGIVGGPIVKRIYDSQAIYALVMAGICMLIASISVIFVDDKDDP
                     VLLRKQKNVVFR"
     gene            complement(3507..4568)
                     /locus_tag="PLF20_08295"
     CDS             complement(3507..4568)
                     /locus_tag="PLF20_08295"
                     /inference="COORDINATES: protein motif:HMM:NF019365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HOX81038.1"
                     /translation="MILLFFLLPACSKESNPQPEPEPDAPSFFIRGADLSLLPDIEKY
                     GIQLYDINGNISDMLSIIKAAGCNTVRMRLWYNPADENSSFEQVKDFAARVKAKGMDV
                     WLCIHYSDTWADPGNQKTPRAWNYLHLAELKDSVYQYTYRVVSAIQPLYVQTGNEVND
                     GFLWPLGRISTGGSFYELMTEAVKAAKAAAPAAHLMIHYAGIDGVALFFNQLKYKNVN
                     FDVIGLSYYPYYHGKDMSYLTSVMNSLSKNIQRPIIIAETSYPFTLQWNDLSHNLIGT
                     ADQLIDSFPASPEGQRAYMIRLRHIMEGVKLGSGFCYWGGEWVAYKGPDATDGSPWEN
                     QALFDFEMKALPVLDVFKK"
     gene            complement(4698..5948)
                     /gene="hutI"
                     /locus_tag="PLF20_08300"
     CDS             complement(4698..5948)
                     /gene="hutI"
                     /locus_tag="PLF20_08300"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOX81039.1"
                     /translation="MIIITNIQQLVNVDESGRAVRVGKDMQKLNTISNAWLLIEGDEI
                     RGYGEMDSSEFQQFVNLPDIEVIDADKGMVFPSFCDSHTHLVYAGSREQEFVDKIRGL
                     SYEEIARRGGGILNSSRLLHETSEEQLLEQALFRLDEIIHQGTGAVEIKSGYGLNTED
                     ELKILRVIRRLKELSPLTIKSTFLGAHAIPLEYKDTPDDYVDVIIQEMIPQVASEDLA
                     DFIDVFCDRGFFTPEQTDRILMAGMKYGLKAKIHANELDLSGGVQVGVKYNALSVDHL
                     ENVGDEEIECLLQSETMPTILPGAAFFLNMPHAPARKMIDAGLPVAFASDYNPGSSPS
                     GNMQLVLSMACIQYRLLPEEAILATTLNSAYAMGVEDILGSIAKGKKANLFITHPIPS
                     IYFMPYAFGTNKVKHVILNGKKIF"
BASE COUNT         2066 a         1381 c         1215 g         1784 t
ORIGIN      
        1 tataatccga ttttttaaaa cgccattgcc agttgttgga cgatgttcca ggtgtattca
       61 ttcgggctgt atagtcgagt tcgaggatat cctgcatggg agcaacagcg atgaaagcaa
      121 ccgaactcca tgccaatcga ataaatttcc aaacggtatt ttcgtttcct cctgacaaat
      181 agcgccaaat tcctgattta gtcgcaccat ccagttcgtt gtaccagcca agagtcgtat
      241 tgttgtcgtg agtacccgtg taacaaatat aattggaggt attatagttg tgaggaagat
      301 attcgttgtt tttcatcggt gaaaaggcaa actgcaaaac tttcattcca ggaaagtgaa
      361 agcggtctct caattcttcg acatcggggg ttataacccc aagatcttcg gcaataattg
      421 gcaaattgcc aagcttttgc tgtatggttt cgaatagctc ggcacccggt gcatctatcc
      481 attttccgtt aatggcagtg gtttctccga aaggaacagc ccaatatgcc gcaaatcccc
      541 taaaatgatc gattcgtata aaatcataaa gcgaaagagc tgcttttatc ctatctaacc
      601 accaggcgaa agcagtttgt tttaaataat cccagttgta tagcggattt ccccatagtt
      661 gtccggtctc agaaaaataa tccggtggaa caccagctat aaatgtagga ttatgatgtt
      721 cgtcgaactg gaaaacttcg ggatgcgacc atgcgtcggc actgtcgtga gcaataaaaa
      781 tgggtatgtc accaattatt cttatcccat tttgattggc gtaagccttc agggcactcc
      841 attgctcgaa aaacacaaac tgtaagaatt tccagaattc tatttcttgt tgacgtgaaa
      901 cgataaattc atccagagca atccgatggc gcatcttgta aggttcatcc cactgatgcc
      961 aaggtcttcc tccatgaaaa gatttcaatt ccataaagat ggcgtaatcg tcgagccatg
     1021 cttgattttc agaacaaaaa ttgctaaaaa caatcttttc atctattgaa gccacatcct
     1081 taaaaccttg aaaagctgaa cgcagagttt tggatttgaa ttcgatgaca gatccgaaat
     1141 caacgtagtc gtcattccca ggagcatcat cgaattctga tggggtcaac catttttttt
     1201 cgataagttt ttcgaagtcg atgagcaaag gatttcccgc aaaagttgaa aaactttgat
     1261 agggtgaatc gccgaatcca gtagggccta aaggaaggat ttgccaaatt ttttgcttag
     1321 ctttcactaa aaaatccaca aaatcaaagg cattcttacc taaagttcca atgccataaa
     1381 gccctggcaa agaagtaata tgcaataaaa ttccactttt tctttctatt ttcatgattt
     1441 aatttttaaa aaacgttgat ttgatgagcg attcgaggtt cttattagac gttcatcgaa
     1501 gaacgacaaa tcactcaata tttatttcta tctaaaaacc acattttttt gtttacgtaa
     1561 aagaacggga tcatccttat catccacaaa aatgacggag atggaagcaa tcaacataca
     1621 aatgcctgcc attaccagag cgtatatggc ttgagaatca tagattcgct taacgatagg
     1681 gccgcctact attccgttga tgatctgtgg gatagtgatg aagaaattga agattcccat
     1741 ataaaccccc attttttgtg cgggaagcga acctgccaga atcgaataag gcatgcttag
     1801 aatacttccc catgcgatac ctattccaac cataggaagc aacaaccact tgggtgaagg
     1861 aacaaaataa atggaaatca atccaatgcc tcccagaatc aaagaaagag aatgcgtaag
     1921 cttacggccg attttgccgg caatggatgg aataataaaa gcatataagg ccgaaatggc
     1981 attgtaaact ccaaaacaga ttccaaccca gtttccggca ttctgataaa tttccgatga
     2041 acgatcgtca atagctgcac cataaatatg tacggccaca gcaggtgtag taaacaccca
     2101 catcgagaac aatgcaaacc acgaaaagaa ctgaactaca cctaattgtt tcatggtgcg
     2161 agggatatga gcaaaatcgc gaaaaatctc tataaatccg cctttagtct gttcatcggc
     2221 cggaatatca gagaattgtc gcaactgttc gggaggatat tctttagtac gtatgactgt
     2281 ccagagtaca cacgagataa atactgcagc ccccacataa aacgaaaaaa ccacattatc
     2341 aggaacctga ccctcaggag cagtttttga aatgcccaac cattcagcaa aaatataggg
     2401 aagccatgag cctataacag cgcctatgcc aattaaggcg gtttgaatgg aaaaaccaag
     2461 agttctctgt tcagtaggca gcatatcggc caccagtgct ctgaatggtt ccattgccac
     2521 attgatggaa gcatccaaaa tcataagaat acccgcacca ataaacatgg gtggaataac
     2581 actggtcagc agttcggcat tgggcataaa aacaagagct aaagaagcaa gtatggctcc
     2641 ggcaagaaaa tagggacgtc ggcgacctaa cttacaccat gtcttgtcac tgtaatggcc
     2701 aataataggt tgcactatca ttcccgtaag aggagcagct atccagaacc atgacaaatg
     2761 ctcaacatca gcaccaaaag tctgtaaaat gcggctggca ttcgcatttt gcaaagcaaa
     2821 accaaactga atgcccaaaa aacccacact catattccaa atctgccaga aactcagttt
     2881 aggcttggaa tactgttttg cttgtaatga actcattatt agtagttatt tgaatcggaa
     2941 ttgggaaaga acagaatcct gataaaatcg caaatgtaaa agtttttgaa atgaggatga
     3001 cagattttgg ggattgataa atgggatatg gagtatcgtg agctaaaagt ttataaaaga
     3061 gaaagggcga aagagacgag gagacgggga gacgagggga attgggagaa ctggtagaga
     3121 tcacatgctt ggagattttg agtatggttt acaattagcg ctctactttg cggagctttg
     3181 caaaactaaa ggttctttac agttaaaaat ggagaaatga aagatggaga ttagttaacc
     3241 atcaatatta agcataaaaa ttaattatga attaattgca caaaatctcc gaattgatat
     3301 ttataaacct cggtagaaca aaatagatga aaatccccct cttaaacctt gtaacctttt
     3361 ctattcttat ataaatacct gatataaaac agtttataaa attgtaagta gaacaggtgt
     3421 aaatagaaat taattttcct caattttctg aaaataactt tccacacttt tgtaaaaagg
     3481 tcaataaaca atgcacttgg ttctatttat ttcttgaata catcaagcac gggcagagct
     3541 ttcatctcga aatcaaataa agcttgattt tcccatggag aaccatcggt agcatcgggt
     3601 cctttgtaag ccacccactc tcccccccaa taacaaaaac cagaccccag cttcactcct
     3661 tccataatat gacgaagcct tatcatgtaa gccctttgac cttcgggact tgccggaaaa
     3721 gaatcgatca actgatcggc agtacctatc agattgtgag aaaggtcatt ccattgcagt
     3781 gtaaatggat aagaagtctc ggcaatgatg atcggccgct gaatattttt actcaaggaa
     3841 ttcatcacag aagtcaagta actcatatct ttaccatgat aataagggta atacgaaagg
     3901 ccaatgacat caaaattcac attcttgtat ttcaattgat tgaaaaacaa agctactccg
     3961 tcgatacccg cataatgaat catcagatgt gcagcgggtg cagccgcctt tgctgccttt
     4021 acagcctcag tcatcaattc atagaatgaa ccgccagtgc ttattcttcc taatggccac
     4081 aagaaaccat cattcacttc attgccggtt tgaacatata atggttgaat ggcacttact
     4141 actctgtaag tgtattgata aacactatcc tttaattcag cgaggtgtag ataattccaa
     4201 gctcgaggcg ttttttgatt gccgggatcg gcccaagtat ccgaataatg aatgcacaac
     4261 caaacatcca tacctttcgc cttgaccctt gcagcaaaat cctttacctg ttcgaatgaa
     4321 gagttttcat ccgccggatt gtaccacaaa cgcatcctaa cagtattgca gcctgcagcc
     4381 tttattatac ttagcatatc agaaatattg ccgtttatat cgtaaagctg aattccatat
     4441 ttttcgatat cgggaagcag ggataaatcg gctccacgga taaagaagga aggtgcatcg
     4501 ggttctggct cgggctgcgg attagattct ttggaacagg ccggtaaaag aaaaaaaagc
     4561 aaaatcatga tcgaataaaa tatattcttg taaaatctct tcatcatctt ttttaaatgg
     4621 tcgattataa cacagatcgc tttcttaaca caaattaact catgaagcaa accccaaatt
     4681 tcgagggatt ggtttagtta gaaaattttc tttccattca ggatcacatg tttcacctta
     4741 ttagttccga aggcataagg cataaaatag atagaaggga tgggatgagt aatgaaaaga
     4801 ttggcttttt tcccttttgc aatggagccc aagatatctt ctacacccat ggcataggcc
     4861 gaattgaggg tagttgccag aatagcttcc tcgggcaata aacgatactg aatgcaagcc
     4921 atagaaagaa ccagctgcat attccccgat ggcgatgatc ccggattata gtcgctggca
     4981 aatgcgacgg gcaggcctgc gtctatcatt ttacgggcag gagcatgagg catattcaaa
     5041 aaaaaggcag ccccaggaag tatcgtcggc atcgtctcgc tctgaagcaa acactcgatt
     5101 tcctcatctc caacattctc gagatgatcg accgaaagcg cattgtattt cactcctact
     5161 tgcacccctc ccgataaatc gagctcattg gcatgtatct tcgccttcag accatatttc
     5221 atacccgcca tcaaaatgcg gtcggtctgt tcaggagtga aaaatcccct atcacagaaa
     5281 acatctataa aatcggcaag gtcttctgaa gctacctgag gaatcatctc ctggataatg
     5341 acatcgacgt aatcatcggg ggtatcttta tattccaaag gaatagcatg agcacctaag
     5401 aaagtggatt ttatagtaag agggctcaat tctttcaaac gtcggattac acgtaaaatc
     5461 ttcaattcat cttcggtatt caatccataa ccgcttttta tttcaacagc accagtacct
     5521 tgatgaataa tctcatccaa acgaaataga gcttgctcca acaattgttc ttcgctagtt
     5581 tcgtgcaaca accgagacga attcaaaatt ccccctcccc ttctggcaat ctcctcatac
     5641 gaaagaccac gtatcttgtc cacaaattcc tgctctcggc tgcccgcata caccaaatga
     5701 gtatgcgaat cgcaaaagct agggaaaacc atccctttat ctgcatctat cacctcaatg
     5761 tccggtaagt tgacaaattg ttgaaattca gacgaatcca tttctccata ccccctgatc
     5821 tcatctcctt cgataagtaa ccaagcgttt gaaatcgtgt tcaacttctg catgtccttg
     5881 ccaactctaa ctgctctgcc actttcatct acattcacca gttgctgaat attggtaatg
     5941 attatcatgt ttttagggtt aatttgaatg aattgattgt tctgatttaa gtcgatttca
     6001 gtatagattt atttcaaata tattgaacaa gcatgttctt gcaatagcct gccttccccg
     6061 atgattcaga attccgttgc aagcaagcca ctgcaaatct tttactcgaa gcaccataca
     6121 aaaagacatt tgagctcaaa ttttctaagc atcaaaggta agatattcga gcgaaccaaa
     6181 tccaaactcc gacctcttac tccaattctt ttccataaat ctctatggcg ttcaatgcaa
     6241 acctcaatca ttcaaattgg ctctaatcaa tctggtttaa gcttaaaaat ctgtccatac
     6301 atcttcaaag attctgagtg atattaagtt ttattctaaa ctaatgcgaa tttttaaaat
     6361 ttaatcgatt aaccttattt atccctacgg gacttatccc aacttgagca ttttaccctg
     6421 aaaaaacagg aaaaaaatag agtttt
//
LOCUS       DAOORU010000137         6374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_14866_length_6374_cov_6.032125, whole genome shotgun sequence.
ACCESSION   DAOORU010000137 DAOORU010000000
VERSION     DAOORU010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6374
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14866_length_6374_cov_6.032125"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1479)
                     /locus_tag="PLF20_08305"
     CDS             complement(127..1479)
                     /locus_tag="PLF20_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX81040.1"
                     /translation="MKFQIFKITASAKKSRLFFLIFCVTAFASVHAQEVYNLQRCIAT
                     ALEQNYNIKIVCNEEKIASNNFTRGNAGFLPTIKATTGHEGSSLTTHQTFREGNESNS
                     KTLFTPSADAGINLNMTLFQGYRAQTTYHKLDELKQLGELDTQMAIENLISQIISEYY
                     YYIQQLNLYKNLEYAVQLSRERVRIDEERYILGSSSKVDLLQSIVYLNSDSSRFSKQN
                     EVLRTSQIRLKKLMGENQIEGEFLVIDTSIPINANLDYDELLDNTLSHNTALQIASKN
                     KTITGLDYQIISSRSYPYLNLTAGYNYSYNGYETGSLKNQQVYGFNYGLTLGMNVFDG
                     SNQKREKANARLEVENKEHNYDDILLQIKADLVTIYYAYQNNLKLLQLEQQNLAVARE
                     NLEIAMERYKLGNLSGLELREVQKSLLDAEERLISVKYQTKLAEISLFQISGKLLEYL
                     "
     gene            complement(1460..4504)
                     /locus_tag="PLF20_08310"
     CDS             complement(1460..4504)
                     /locus_tag="PLF20_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOX81041.1"
                     /translation="MSLSSTSINRPVLATVFSLIILLLGGIGITFLGIREFPSVDPPI
                     ISVSTSYPGANSDVIETQITEPLEQTINGIPGIRTLTSVSSQGFSRITVEFELSVDME
                     AAANDVRDKVSQAQRFLPRDCDPPIVSKADADARPIMHIAIHSPKRSLLELSEIAELT
                     FKEQLQTISGVSAVNIWGEKRYAMRIWLDPSKLAGYQMTPLDVRNSLQRENIELPAGS
                     IEGNNTELTIRVLGLMSSPEEFNNLILKRSGDQLIRVKDIGRAELSPEDLRSIMKMNG
                     IPMVATVIVPQPGANQIEIVDEVYKRLDFIKKDLPQDIGIEFGFDNTQYIRSSIKEVQ
                     TTIYVAFFLVVSIIFLFLRDWRTTLIPILAIPVSLIGTFFIMYLAGFSINILTLLGIV
                     LSVGLVVDDAIVMMENIYVKIEQGMSPWEAAIKGSNEIFFAIIATTITLIAVFFPIVF
                     LQGMTGRLFREFSVVIAGAVAISAFVALTFTPMLSSKMLKARPSHGKLYDFTEIFFIR
                     LNAGYKNSLDNFLRRKYLPLTIVGGTIVLIIVLWKVIPAEMAPLEDRSQINLRMIFPE
                     GSTYDYSFAYTEEISALVDKLVPEAEKSTMRVWTGGSSIEIVLVPPSKRKRSQQEIAN
                     QLTTVLGKKTKARVNVLQQSTFGGRRTALPVQYVIQAQNIDKLREVLPEFMARVQDNP
                     YFTMADVNLRFTKPELQAQINRDKANILGVSTQNIGQTLQLALSGQRFGYFIMNGKQY
                     QILGELPRNDRNEPLDLKSLYVRNDAGQMVQLDNFVELKEATAPPQLYRYNRFVSATI
                     SAGLAEGKTISEGIEEMDKIAAGVLDDTFRTALSGDSKDFMESSSSLVFALTIAIVLI
                     FLVLSAQFESFKDPVIVMMTVPLALAGALVFMWYFNVTMNIFSQIGIIMLIGLVSKNG
                     ILIVEFANQRKMAGMSKSEAIRYAAAARFRPILMTSLSTILGILPLALGLGEGAQSRV
                     AMGIAVVGGLTISTFLTLYVVPGIYQIISSETKNNGNEISNL"
     gene            complement(4501..5631)
                     /locus_tag="PLF20_08315"
     CDS             complement(4501..5631)
                     /locus_tag="PLF20_08315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOX81042.1"
                     /translation="MTLRSKRIIRITMTIAAGVVILPLIFYPKIKPLWNKASEGGQLM
                     PQRGAGRGNQPLFASGYVIIPVQMSEMIRSTGTLLPDEEVELTFETQGKVVGIYFDEG
                     RRVKKGELLAKLNDAPLQAQLLKLQAQHKLTEEKVFRQKQLLDRDAISRESYDQVATE
                     LQTIEADIKLLEARISETELRAPFDGIIGLRLISEGAYATTQTPLARLVKISPLKLEF
                     SIPERYAGDVSPGFPVKFSIDGISKEFQADVYAIDPKIEINTRTIVVRAYYPNKNEEL
                     KPGRFTSVSLLLSQIDNAVAVPSEALIPEMNGVKVFVYRNGKAEQVRVTTGLRTESQI
                     EIKSGLKFGDTLLTTAILQLRQGIPVKLDTLIINNENLRTNL"
     gene            complement(5983..>6374)
                     /locus_tag="PLF20_08320"
     CDS             complement(5983..>6374)
                     /locus_tag="PLF20_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81043.1"
                     /translation="LPKNSFKGSSDGIIYSIIIGMACCFVILIYYILSPTMKNLPYYN
                     PMITTMGLVSVIFAIFMGFFTGLGKARKNKFVDSMTGLFAAAFIHGLYKFCFYTEEYT
                     LGVIAAIAELIIAIILIIISVRTQEVS"
BASE COUNT         1837 a         1312 c         1301 g         1924 t
ORIGIN      
        1 ttttatcact aaatttgtct aaccaaaaag ttgcatttgt gacttgaatt cagcatttat
       61 aagcttttaa ctttcaactt tttaaattct attcatcatt caccattttt ctcttagact
      121 tacttactac aagtactcaa gtaattttcc tgaaatctgg aataaggaaa tttcggccag
      181 cttggtttga tatttcaccg aaatgagccg ctcttcggca tcgaggaggc ttttttgaac
      241 ctctcttaat tcgagccccg ataaattgcc caatttatat cgttccatgg caatttcgag
      301 gttttcacgg gctacagcta aattttgctg ctcgagctgg agcaatttta aattattctg
      361 ataagcatag taaattgtaa ctaaatcggc ctttatctga agcaaaatat catcgtaatt
      421 atgctcctta ttttcgacct cgagtcgtgc attggctttt tcacgttttt gattggaacc
      481 atcgaacaca ttcatgccca acgtcaatcc atagttaaat ccgtaaacct gttgattctt
      541 caaactacct gtttcgtacc cattatagga ataattatat ccagccgtaa gattcaaata
      601 aggatatgaa cgtgaagaga tgatttgata atcaaggccg gtaatagttt tattcttcga
      661 ggcgatttgc agagctgtat tatggcttaa agtattatcg aggagttcat cataatcaag
      721 attggcattt ataggaatac ttgtatctat tacgagaaat tcaccttcaa tttggttttc
      781 tcccatcaat ttcttcaatc ggatttgaga agttctcaat acttcgtttt gttttgaaaa
      841 cctcgaacta tcggaattca aatacacgat ggattgcaag agatcaactt ttgacgaaga
      901 tcctaatatg tagcgttctt cgtcgattct gacgcgttcg cgcgaaagtt gcacggcata
      961 ctccaggttt ttatagagat tgagttgttg aatgtaatag taatattccg agataatttg
     1021 tgagataaga ttttcgatag ccatttgggt atcgagttca ccgagctgtt tgagttcatc
     1081 gagtttatga taggtagttt gtgcccgata tccctgaaac agggtcatat tcaggttgat
     1141 gccggcatcg gccgaaggag tgaataaagt tttactgttc gattcgttac cttcgcgaaa
     1201 ggtttggtga gttgtaaggg agcttccctc atggcctgtt gtagccttaa tcgttggaag
     1261 aaatcctgca tttccgcgtg taaaattatt gctggcaatt ttctcctcgt tgcaaactat
     1321 ttttatatta tagttttgct cgagggcggt tgcaatgcag cgttgcaaat tgtaaacttc
     1381 ctgtgcatga actgatgcga aggcagtcac acaaaatatc agaaaaaata atctcgattt
     1441 ttttgctgaa gcagttattt taaagatttg aaatttcatt gccattattt ttagtttcgc
     1501 ttgaaataat ctggtagatt ccaggtacca cgtacaaggt aagaaaagta gaaatggtga
     1561 ggccacccac tacggcaata cccatagcta cacggctttg tgccccttca cccagaccga
     1621 gtgccagagg tagaatgcct aaaatggtag aaagactggt cattaaaata ggacgaaatc
     1681 tggcggctgc agcatatcgg atggcctcac tcttgctcat tccggccatt tttcgctgat
     1741 tggcaaattc cactatgaga attccgttct tcgaaaccag tcctatcagc atgatgattc
     1801 ctatttggct gaagatgttc atggtaacat taaaatacca cataaaaact aaggcgcctg
     1861 cgagtgcaag aggaacggtc atcataacga tgacggggtc tttgaaactt tcgaattggg
     1921 ccgagagtac cagaaagata aggactatgg ctatagtaag ggcaaacacg aggctagatg
     1981 agctttccat aaaatctttc gaatcgccag acaaagccgt gcggaaggtg tcgtccaaaa
     2041 caccggcagc aatcttgtcc atttcctcga ttccttcact tatggttttt ccttcggcca
     2101 ggcctgccga tatggtagcc gaaacaaaac gattgtagcg gtaaagctga ggaggggcag
     2161 tagcttcttt caattcgacg aaattatcga gctgaaccat ttgccctgca tcgtttctta
     2221 catacaaaga tttgaggtcg agaggttcat tccgatcatt gcggggaagt tctccaagaa
     2281 tttgatactg tttcccattc attataaaat atccaaatcg ctgtccactt aaagccagtt
     2341 gcaaggtttg accaatattt tgtgtcgaaa cgcccagaat attggccttg tccctattaa
     2401 tttgggcttg cagttcaggt ttggtaaatc tcagattgac atcggccata gtaaagtatg
     2461 gattatcctg aacgcgtgcc ataaattcag gcaggacttc tctcaattta tctatattct
     2521 gagcctgaat aacgtattga acaggaagcg cagtacgtcg tccaccaaaa gtggattgct
     2581 gtaatacgtt cactcgcgct ttggttttct tacccaagac agtcgtcaat tgattggcaa
     2641 tttcttgttg agaacgtttc ctcttagaag gagggaccag aacaatttcg atggaagagc
     2701 cacccgtcca aaccctcatg gttgactttt ctgcttcagg aacgagttta tccaccaaag
     2761 ccgaaatctc ttcagtataa gcaaaactat aatcataagt cgaaccttcg gggaaaatca
     2821 ttctcagatt gatctgggaa cggtcttcca gaggagccat ttcggccggt atcactttcc
     2881 ataggacgat tatgaggaca attgtacccc ccactatcgt caatggtaaa tatttcctgc
     2941 gcagaaaatt atccagtgaa tttttatatc cagcattcaa tcgaataaag aatatttcag
     3001 taaaatcata gagttttcca tgtgacggcc tggctttcag cattttcgac gaaagcatgg
     3061 gagtaaatgt aagcgctaca aatgccgata ttgccactgc acctgctatg acgacactga
     3121 attcacgaaa gagccttcct gtcatgcctt gcaggaaaac tatgggaaag aaaactgcaa
     3181 tcaaagtaat ggtggtagct atgatggcga agaaaatctc gttcgatccc ttgatggctg
     3241 cttcccatgg gctcatgcct tgttcaatct ttacatagat attttccatc atcactatgg
     3301 catcgtcaac caccaaacct accgaaagta ctattcccaa cagggtaaga atattaatgc
     3361 taaagccagc cagatacatg ataaaaaacg taccaatgag tgaaactggg attgcgagaa
     3421 taggaataag ggtagtacgc caatcgcgca agaaaagaaa aataatagat actacgagga
     3481 aaaatgccac ataaatggtg gtttggactt ctttgatgga tgagcgtatg tattgtgtgt
     3541 tatcgaaacc aaattcgatt ccgatgtctt gaggaagatc ttttttgatg aagtccaatc
     3601 ttttgtatac ttcatccacg atttcgattt ggttagcgcc gggttgaggc acaattaccg
     3661 tagcaaccat aggaattccg ttcatcttca tgatgctacg gaggtcttcg ggcgaaagtt
     3721 cggcacggcc aatgtctttt actctaatca gttgatcgcc tgaacgtttc aggataagat
     3781 tattgaattc ttctgggctg ctcatcaatc caagaacacg tattgtaagt tcggtattat
     3841 ttccttcgat gctacccgct ggcaattcta tattttctct ttgcaaggaa tttctaacat
     3901 cgaggggtgt catttgatat ccggccagtt tggaaggatc gagccatata cgcatggcat
     3961 atcgtttttc tccccaaata tttacggcac tgactcccga gatggtttgc aactgctcct
     4021 taaaggttag ttcagctatt tcagatagct cgagcaacga tcgtttagga ctatggattg
     4081 caatatgcat gataggtcgg gcatcggcat cggctttcga aactataggc gggtcgcagt
     4141 cgcgtgggag aaatctttgt gcttgcgaaa ccttgtccct tacatcatta gccgcggctt
     4201 ccatatcgac cgataattcg aattcaacag taattcggct aaatccctga ctgctaacgc
     4261 ttgtcaaagt tcgtattcca ggaattccat tgatggtttg ttcgagaggc tcggtaatct
     4321 gggtttctat cacatccgaa ttagcacccg gataacttgt acttaccgaa atgattggag
     4381 gatcgacgct gggaaattcc ctgatgccta aaaaagtaat tcctattcct cccagtaaaa
     4441 gaatgatgag agaaaagacc gtagctaata caggccggtt aatgctggtg gaagaaaggc
     4501 tcataggttg gtgcgcaaat tttcgttatt tattataaga gtgtcgagtt ttacgggtat
     4561 accttggcgg agttgcaaga tagccgttgt aagcagcgta tcgccaaatt tcaaacccga
     4621 ttttatctca atctgtgatt cggtgcgcaa acctgtagta actctaactt gttcggcttt
     4681 cccatttcta taaacgaaca cttttacacc attcatctcc gggataagag cttcggatgg
     4741 aacagctacg gcattgtcga tttgtgaaag aagaagactt acactggtaa atctccccgg
     4801 cttgagttct tcgtttttat ttgggtaata agcccttacc actatagttc gggtattgat
     4861 ttcaatcttg gggtctatgg cataaacatc ggcctgaaat tcttttgaaa ttccgtcgat
     4921 ggaaaattta accgggaatc ctggtgaaac atcgccagca taacgctcgg gaatagaaaa
     4981 ttcgagtttc aagggactga tttttactaa tcgagctagg ggtgtttgag tggtagcgta
     5041 ggcgccttcg cttatcagac gcaatccgat gattccatcg aaaggagcac gcagctcggt
     5101 ttccgaaatt cgcgcttcta agagtttaat gtcagcttct atggtctgca actcagtagc
     5161 tacttggtcg taactttcac ggctaatggc atcccggtct aataattgtt tctggcgaaa
     5221 aaccttttct tcagtcaatt tatgttgagc ctgtagcttc aacaattgag cctgcagagg
     5281 tgcatcattc aatttggcta aaagctcacc cttcttcacc cttctgcctt catcaaaata
     5341 aattcctaca actttgcctt gtgtttcgaa agttaattct acttcttcat caggaagcaa
     5401 tgtacccgtg ctacgtatca tttcgctcat ctgaaccgga atgatgacat aaccactcgc
     5461 aaacaaaggt tgattgcctc ttccagcacc acgctgcggc atcaattgac caccttcaga
     5521 agctttattc catagtggct tgatcttggg ataaaaaatc aagggaagta tgaccacccc
     5581 tgcagcaatc gtcatcgtaa tccgtattat tctcttcgat cttaatgtca taaaaacctc
     5641 attatttgtt atagaagaac atattcaggg gacttcaaat ttcgtaatat tattaaagaa
     5701 gagctatgga caacccctcc aatagtatct acctcatata tagttaattg aactcttatt
     5761 ctaatttcaa tgaatggtga aaaatattac aaaatcttca tctttggata ttatttcaaa
     5821 agaatggttt attgagaaaa gggaaaaatg gcatcccatg attcagcatc ccgaaccatg
     5881 gagccggttg gttcattcga atttgatata actccaaacc caatttgctc ttgacgaaag
     5941 atttgcatag gcgttttaac catacacaaa cactttactg tatcaagaaa cttcctgggt
     6001 tctcaccgaa attatgatta aaattatggc tatgatcaat tcggctatag cagcaataac
     6061 tcctaaagta tattcctcag tgtagaaaca aaacttataa agtccatgaa tgaatgcggc
     6121 tgcaaacaat cctgtcattg aatcgacgaa tttattttta cgagccttgc ccaagccagt
     6181 aaaaaatccc atgaatatgg caaaaataac acttaccaga cccatagtgg taatcatggg
     6241 gttataatat ggcaggtttt tcatagtagg gctcagtata tagtatatta gaataacgaa
     6301 gcagcatgcc atgccaataa tgatagaata gataatgcca tccgatgaac ctttaaaact
     6361 atttttaggc aagg
//
LOCUS       DAOORU010000138         6248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_15225_length_6248_cov_4.387696, whole genome shotgun sequence.
ACCESSION   DAOORU010000138 DAOORU010000000
VERSION     DAOORU010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6248
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15225_length_6248_cov_4.387696"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(475..840)
                     /locus_tag="PLF20_08325"
     CDS             complement(475..840)
                     /locus_tag="PLF20_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81044.1"
                     /translation="MYRSKNELTLKTNQLPKTLLNRDTFDLSNKAYKSIIIEAERFDH
                     NLTLYYGMISGECEDEIEYLRKVKRFNHEVSNMDVEHLNDLFFGEPFNKKKLYATIKK
                     VNDNLDKIRQIPKDKRHYE"
     gene            complement(1175..2686)
                     /locus_tag="PLF20_08330"
     CDS             complement(1175..2686)
                     /locus_tag="PLF20_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81045.1"
                     /translation="MEKRGKTFKSETFIFIILIILLIIYRFWILINFGFKYTDSDQTI
                     MWLATVDYYHKSFHEPCFYGQSYNTMLESFLAIPLYAVGCPLYIALPVVTSLLTLFPF
                     IYISILTFIKRSKKTAILLLSLTFLLPIEYDLLTCLSRGFVTGIAIVSLSFHSLFRPF
                     KRNSFFLFGLLSAIGFIVNSNSILLSMPCLLFLFLNNFKNKYFYIEIIMSLLCGFVLF
                     YLMKSFYIYHPNYNLHKYDYKFSWSFFVNGFSSLDKLFNYITPIFWKQGFVVLFIPII
                     FTAYFFKRKMHIEALVSASLSVLILLSLFVSKVYNGTTSIFFPYARMYLAIPLVLVVL
                     LSFIRTRNKYFTIIFVLISVFILSYKFIYTKKEIKECMNRSKSVIGVMDTKKLLSECE
                     ELSTISQKYNIDLMVISFHWNYDFYNYGCPACIEDFPKTLRPAYERRTWRLIEDENYV
                     YENLMIIDLKRDLSNEFDFVSKIQEKSGFYLIKNNILPTKLLLDKLNITVRPY"
     gene            3442..3879
                     /locus_tag="PLF20_08335"
     CDS             3442..3879
                     /locus_tag="PLF20_08335"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOX81046.1"
                     /translation="MFSDEYFMEEAIKEAKAAEEEGEVPIGAVVVWKKKIIARSHNMT
                     ERLNDVTAHAEMLAITAATNYIGGKYLKGCTLYVTIEPCPMCASALAWAQLGKIVFGA
                     HDPKHGYSLISSSLLHPQTVITSGIREKECASIMSEFFKKRRI"
     gene            complement(3880..4275)
                     /locus_tag="PLF20_08340"
     CDS             complement(3880..4275)
                     /locus_tag="PLF20_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81047.1"
                     /translation="MLNYSAKLILAFGETITGNKKILDWLLNNGYPDLAALSYSIRGS
                     EEAFKFLLKYYPRLAALDAAIDNNEKAYLWLKEHNYPFNIIFADACHGKPEALKWLMN
                     HHLEGFIGVTEKIRYFRENQYFDIHKPPF"
     gene            complement(4277..5056)
                     /locus_tag="PLF20_08345"
     CDS             complement(4277..5056)
                     /locus_tag="PLF20_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOX81048.1"
                     /translation="MSTFLFNRIIFGPVRSRRFGKSLGINLLPVTHKVCTYNCIYCEC
                     GLTLEEIEKNREWPAREEVKNKLELWLENMSRNNEPLPDNITFAGNGEPTLHPAFPGI
                     MDDVLELRDRYCPRTRVTVLSNATIANRPLVRQALTKADNNVLKLDTGTETMFRLLNR
                     PPKNLKLEEVIETLTLFKGQLIIQSMFVQGYINGQYIDNTVPGETTPWLEALLRIKPK
                     EVMVYSIARGTPVEGVKPVPFMQLVKIANMVKIARIQAEVY"
     gene            complement(5151..5837)
                     /locus_tag="PLF20_08350"
     CDS             complement(5151..5837)
                     /locus_tag="PLF20_08350"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOX81049.1"
                     /translation="MEEIQAIAMPGTHKRFAEYFLAHETPASKVLDIGAGYGAFSQLL
                     YREGFQVEACDLFPENFQFQSISCHFINLMNRFPFPDQSFDLAVAIEVSEHITDHEMF
                     FSEIERILKPGGRFYISTPNILSLKSRIRFLFTGYYYSFSKLRYDLHDGLQHVASLTL
                     DQYNYIAINHHFHPAEVNIDKQQRTSIWLRIFLIPLLFFFSNLWKTGWFHNQNSLLLG
                     RLLFLCFKKS"
     gene            5981..>6248
                     /locus_tag="PLF20_08355"
     CDS             5981..>6248
                     /locus_tag="PLF20_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOX81050.1"
                     /translation="MLPDLDKIKAPVADELEAFEQSFRMALKSNTRLLDLVLKYLLRQ
                     KGKRIRPLFVFLSAAATGGISPSTQHVAALIELLHTATLVHDDVV"
BASE COUNT         2131 a         1001 c         1042 g         2074 t
ORIGIN      
        1 tctcattgtg tgattgttgt aaaaatgtct tttaaaatgt gtcgaagtca ggaaaagagg
       61 acattgctca ataaacccgc ctttaaaacc catgccttct attagaaaat aaaatttgac
      121 gaaaacggtc tcccattaaa caataaaccc tgcgataagg tatcgagcat aaaaatgttg
      181 tcaacattag aaattactac atcataataa aacggggagg aaataatcat aggcggaagt
      241 atatctctat tttacaaatc gaaatcatac aataaaatat cacccttcac atatagaaat
      301 cctttctttc cctcctaccg caccaaacca ttcaaataaa taaaatgcca actaaccatt
      361 ttatttttat gattttaaat caaaatgttt cataatcaat ttattcataa tgaattatgt
      421 ttataccaac actccttacg caaccattgc tgttgatgct gaaaaggaat gactttattc
      481 ataatgccgt ttatccttag gaatttgtcg tattttgtcg agattgtcat ttaccttttt
      541 gatagttgca tataatttct tcttgttgaa aggttcacca aaaaagagat cattcaaatg
      601 ctcgacatcc atgttcgaaa cttcatgatt gaacctcttc actttacgga ggtattcaat
      661 ttcatcttca cattcacctg atatcatccc ataatacagg gtcaaattat gatcgaatct
      721 ttcagcctcg atgataatgc tcttataagc tttatttgat aaatcaaatg tatccctatt
      781 tagaagtgtt tttggtaatt gattggtttt tagtgtaagt tcatttttac ttcgatacaa
      841 atttgtcaga ttttatagag atattttcaa taaaggtaca aatcttttca atttaaaaaa
      901 cactttaaat ttttagaaac gaattaggat ttattcctat aaatatctta catttccaag
      961 atatcgaaaa caatctacat tttttattat actggtagat ataatatcta atcttgacca
     1021 agtaaggtat ataatttatc gagcttaaca ataacaaatg aaatgaacct aaattttacg
     1081 cttgaaatag attgatattt aaaaatatca aaaagaattg aagtataatt ttcaatttat
     1141 tttttgtaaa caattatttg aaagttgggt taagctaata tggtcggacc gtaatattta
     1201 atttgtccaa taataattta gtgggcaata tgttattctt aatgagataa aagccagact
     1261 tttcttgaat tttagatacg aaatcaaatt cattgcttaa atcacgcttg aggtcaataa
     1321 tcattagatt ttcgtataca taattttcat cttctatcaa tctccatgtt cttctttcgt
     1381 atgcaggcct aagagttttt gggaagtctt caatgcaagc agggcaacca tagttataaa
     1441 aatcataatt ccaatgaaaa gaaataacca ttaggtcaat gttatatttt tgagagatgg
     1501 tcgacaattc ctcacattcg gatagaagtt tttttgtgtc catcactcca ataacagatt
     1561 tagatctatt catgcattct tttatctctt tttttgtata aatgaattta taggataata
     1621 taaaaactga tatcaaaaca aatattattg tgaaatactt gtttctagtt cttataaatg
     1681 aaagtaaaac aacgagaacc aatggaattg ccagatacat tctagcgtat gggaaaaaaa
     1741 tagaagtcgt tccattatat accttagaaa caaataatga caaaagaata agaaccgata
     1801 atgatgctga aaccaaagct tctatatgca tttttcgttt aaaaaagtag gctgtaaaga
     1861 taataggaat aaataaaacg acaaaaccct gtttccagaa tataggtgta atataattga
     1921 ataatttatc gagggagcta aacccattca cgaaaaaaga ccatgaaaac ttataatcgt
     1981 atttatgtaa attataattt ggatgataaa tatagaatga tttcattaaa tagaataaga
     2041 cgaaaccgca taataagctc ataattattt ctatataaaa atatttattt ttgaaattgt
     2101 ttaagaacaa aaacaacaaa cagggcatag atagtaaaat cgaatttgaa ttaacaatga
     2161 aaccaatggc tgataataac ccaaaaagga aaaaagaatt gcgtttaaat ggcctgaata
     2221 aagaatgaaa cgaaaggctt acaatggcga taccagtgac gaaacctctc gaaaggcagg
     2281 ttaatagatc atattcaata ggcaatagaa aagttaatga aagtaataaa attgcagttt
     2341 ttttcgagcg tttaataaat gtaagaatag aaatatatat gaagggaaat aaagtcaata
     2401 gtgaagttac tacaggaagc gcaatatata agggacaacc aactgcatat agtgggattg
     2461 caaggaagga ttccagcatt gtattatatg attgaccata aaagcagggt tcatggaatg
     2521 atttatggta ataatcaaca gtggctagcc acattatggt ttgatcacta tcagtatatt
     2581 tgaatccaaa atttattaat atccaaaatc tatatataat caataaaata attaaaatta
     2641 taaaaataaa agtttcactc ttaaatgttt ttcccctctt ttccatgtta atcaataatt
     2701 tgtcaacatt gttggcaaaa tagaattttc caaatataca tgaaaaataa atatattaca
     2761 attgttatat gaattcataa ttgataattc tttaaataca ataagtttat ctttaatata
     2821 tgttttcgaa gaatccaaat ttatataccc accaccaatc atatgtatgc atattttgaa
     2881 aatcaatgaa tttataataa tccatttata agtgaaccat ttaaattgca ttttttgtaa
     2941 tcaattatta actaattaaa tatactttta ttaatatatt ataaattcct atttttttat
     3001 tcgtcaaagc aagcaatgta aaatttaaac atgcaaacct ataaaatttt tactttcgaa
     3061 cccttcttga agaaaaaaat ctatcctttt aaccttaatt ctttaattct ttaaaatcaa
     3121 tctaaagaca attcatcgat ataatctttg agtatatttt gtgttataga agatttgaca
     3181 gcattgttat attgaaagga atggggaatc atatatctta tgatgatatt aattcaaggg
     3241 cataatatta tttaaagtat tgtctattat agctttgttg ttaccaaatg ctaatattta
     3301 ctttcgagtt attccatata cattaaaatg attaaaagaa tagcatgaca ttctttctta
     3361 tttttgcttc aaatttattt ttttattaga agcttctctt tttaaattca attttaatat
     3421 gaaaggaata tggattttta tatattttct gatgaatatt ttatggagga ggctataaag
     3481 gaagccaaag ctgcagaaga ggaaggtgaa gttcccatag gagcagttgt agtatggaag
     3541 aagaaaatca ttgctcgttc gcataacatg acagaaaggt tgaacgatgt gacggctcat
     3601 gctgaaatgc ttgctattac agcggctacg aattatattg gaggtaaata cctcaagggg
     3661 tgtaccttat acgttaccat agagccttgt ccaatgtgtg cttcggcatt agcatgggcg
     3721 caacttggga agattgtttt tggtgcgcat gatcccaaac acggttattc tctcatttca
     3781 tcttctttat tgcatccgca gacggttatc acttcaggca taagagaaaa agaatgtgcc
     3841 tcgataatga gtgaattttt taaaaaacga agaatttagc taaaaaggag gtttgtgaat
     3901 atcgaaatat tgattttcgc ggaagtatct tattttttct gttacgccaa taaaaccctc
     3961 caagtgatga ttcatgagcc attttaaggc ttcgggcttg ccatgacaag catcggcaaa
     4021 aatgatgtta aagggataat tatgctcctt taaccataaa taggcctttt cattattgtc
     4081 gatagcagca tctaaagcag caagacgtgg ataatatttc aaaaggaatt taaatgcttc
     4141 ttcacttcct cgaatgctat acgagagtgc cgccagatcg ggataaccgt tattaagaag
     4201 ccaatcaaga atttttttat tccctgtgat tgtttctcca aaggcaagaa taagtttggc
     4261 actgtaattc aacatctcag taaacctccg cttgtattct ggctattttt accatattgg
     4321 caatctttac tagctgcatg aatggaacgg gtttcacacc ttctacaggt gtacctcgtg
     4381 caatggaata taccatgact tcctttggtt ttatgcgcag caatgcttcg agccaaggtg
     4441 ttgtttcacc aggcacggta ttatcgatgt actggccatt tatatatccc tgaacgaaca
     4501 tgctttgtat gatgagctga cctttgaaaa gggttagggt ttcaattact tcttccaatt
     4561 tcagattttt tggtggtcgg ttgagcaaac gaaacatggt ttcggttcct gtatccagct
     4621 tcaatacatt attgtcagcc ttcgttaagg cttggcgtac gagaggtctg ttcgctatgg
     4681 tggcattcga aagtactgta actcgtgttc tcgggcaata gcggtctctt agctccagca
     4741 catcatccat tattcccgga aaagctggat gcaaggtagg ttcgccattc ccggcaaaag
     4801 tgatgttatc tggcagaggc tcattgtttc tggacatatt ttcaagccat aattccagtt
     4861 tattcttaac ctcttcacgt gcaggccatt ctctgttttt ctctatttct tcgagggtta
     4921 atccacattc acaataaatg caattatagg tacagacttt atgagtaaca ggtaacaggt
     4981 ttattcctaa cgactttcca aatcttcggc ttcttactgg cccaaaaatg attcgattaa
     5041 atagaaaagt tgacatcttt tattgctaaa attccttgca aaaatagttt acatgcttta
     5101 aactgggcat gaatttaccg gaatagatag aatgtcagta aaataatacg tcaggatttc
     5161 ttgaagcata ggaaaagcag tcttcccagt aataatgaat tctgattatg aaaccaccct
     5221 gttttccata agttactgaa gaaaaacaac aaagggatta aaaatatcct taaccatatc
     5281 gaagtccgct gttgcttgtc gatgttgact tcagccggat gaaaatgatg atttatagct
     5341 atataattgt attgatcgag ggtaagtgag gcaacatgct gaagtccatc atgcaggtca
     5401 tatcgaagtt ttgagaaact gtaataatat cctgtaaata agaatcttat tcttgatttc
     5461 agagaaagaa tattgggagt agaaatgtag aaccttcctc caggcttaag gatacgttct
     5521 atttctgaga agaacatttc gtggtcggta atatgttcac ttacttcaat tgccacagca
     5581 aggtcgaaac tttgatcggg gaaagggaac ctgttcataa ggttgatgaa atgacagcta
     5641 atcgattgaa actgaaaatt ttccgggaat agatcgcagg cttctacttg aaaaccttcc
     5701 cggtaaagta gttgggagaa agcaccataa ccggctccaa tatcgagaac tttagatgca
     5761 ggtgtttcgt gggcaagaaa atactctgca aagcgcttat gcgttccagg catggctata
     5821 gcttgaattt cttccataaa taattatttt atattctctg tttttattct ttcaaaatta
     5881 caatcataat ttattgtaag catattttca cattgattag cctgagaatg tactactcat
     5941 cgaaagttgt taattttgaa attcaaagac cggtaatcgc atgttgccag atctcgataa
     6001 gataaaagct cccgtagccg atgaactcga agctttcgag caatctttcc gaatggcctt
     6061 gaagagcaat actcgcttat tggatttagt gctgaaatat cttttaagac agaaggggaa
     6121 aaggattagg ccattatttg tctttttatc ggcagcggcc acggggggaa tttctccatc
     6181 aacccagcat gtagcggctc ttatcgagct actacatact gctactctgg ttcatgatga
     6241 tgttgtgg
//
LOCUS       DAOORU010000139         6235 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_15267_length_6235_cov_7.853398, whole genome shotgun sequence.
ACCESSION   DAOORU010000139 DAOORU010000000
VERSION     DAOORU010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6235
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15267_length_6235_cov_7.853398"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1047)
                     /locus_tag="PLF20_08360"
     CDS             complement(40..1047)
                     /locus_tag="PLF20_08360"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HOX81051.1"
                     /translation="MSTIKIEKIQHKGSEKIRIEFGYDKERIERVKQIPGAQWSQSQK
                     AWHIPDTNEDVEKFNQLFPDSPIVQPTPEIPPKAEPQEPQKARNEIVVEVSAKNIWLK
                     MPKNTADIAFVKTIKFSRWNPKSYRWEMQNYADNLDRIKQYFGKRITQIVTIDEPKRK
                     TNLAETTKISLYDKELPPTTKNQIRQFEQWMIHKRYSESTVKNYISALTTFLRFVLPK
                     IPESITDEDFVRFVNEYLIPNNLGNTTQNQTVSGAKLFFKQIVKTQFDPEVLERPQRE
                     HLLPKVLSKQEVAVIITGTANLKHRTMLSLIYTCGLRRSDVLGLKIEHVDSKRGLLSV
                     M"
     gene            1195..1824
                     /locus_tag="PLF20_08365"
     CDS             1195..1824
                     /locus_tag="PLF20_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOX81052.1"
                     /translation="MKRTCGLDVHKDTIFCATYDGKKHGSVIEYSTLTTSIRAMGAAL
                     LSEEVKEIAMESTGMYWIPVWNILEEMGFNLTLVNPYLIKQMPGRKSDVKDAQWIATL
                     LHKGLLRSSLVPCKEIRELRTYSRRYVKLQQRKTSVLQDMERTLEVCSIRITSLVSNI
                     SGKSVMKVIHALIEGENDPEVFAGYIHGRIVNKHKRKTIVESLRGHVIS"
     gene            complement(2267..3016)
                     /gene="dapB"
                     /locus_tag="PLF20_08370"
     CDS             complement(2267..3016)
                     /gene="dapB"
                     /locus_tag="PLF20_08370"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOX81053.1"
                     /translation="MKILISGYGRMGKEVERSALESGHQIIGIYDNAKDWDDKAELIP
                     QCDVVIDFSLPAEAPRNIIRSIDNHKPIVCGTTGWYDQLSFISNYVGQKHGALFYAPN
                     FNLGVNIFMNLSEILADIMKDYPQYEPAITEIHHTRKLDAPSGTAIALAERLIKNLPQ
                     KYEWTKCKEGEQALPHQIPITSIREDDITGIHTLEFRSKDDRITLTHEAFDRSGFAIG
                     AIMAAQWLLGRKGIFNMNDLLKNHPTITDIA"
     gene            complement(3029..4576)
                     /locus_tag="PLF20_08375"
     CDS             complement(3029..4576)
                     /locus_tag="PLF20_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HOX81054.1"
                     /translation="MFTPNDLQQLASKGISKETVEKQIQYFREGFPWAELVRPATVGD
                     GILRFDDAQISELENYYEKNRHGLDILKFVPASGAASRMFKHLYEFREQYDGSEDALA
                     KLESDRGLQSVYTFIHRIRDFAFSEQLENIFHQKGTSIDKVLEKKDYNLLISCVLEPW
                     GLNYGNLPKGLILFHKYPEGPRTSIEEHLVEGAQHARKNNDEARIHFTVSPEHKEKFE
                     ELITRKAPFYEKKLDVHFRIEYSIQKSSTDTIAVDLNNQPFRNKDGSLLFRPGGHGAL
                     LENLNELEGEIVFIKNIDNIIGDRLRSETIKYKKVIGGLLLKIRNRVDDFLLHLEHTA
                     VDNELLNEVEKFASETLFIYLPDEYKNMSNHAKAAWWKDKLDRPVRVCGMVKNIGEPG
                     GGPFLVKNQSGEISLQIVESSQINLNDPKQKAIMAQSTHFNPVDLVCSLTDYKGRRFH
                     LPNYVDPSTGFISIKSKDDRDLKALEHPGLWNGAMAKWLTVFVETPLITFNPVKTIND
                     LLRPEHQ"
     gene            complement(4591..5337)
                     /locus_tag="PLF20_08380"
     CDS             complement(4591..5337)
                     /locus_tag="PLF20_08380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX81055.1"
                     /translation="MMRLDSKVAIITGASSGIGKAIALELAKEGAKVAIAARHLDFLY
                     KVEKEILQSGKEAIAFPMDVSNKDNWNEFIHKVIESWQKIDILINNAGIMPLSFIRNL
                     RVDEWERMIDVNLKGVLFGTAAVLPYMRSQGYGHIVNISSTAGRWLFDGGAVYCATKF
                     AVNAFSEGFRQEITSTENIRMTLIEPGITATNLPSTINDPDIRASSEDNPPIMPLQPS
                     DIAAAVIYTLTQPPQVNVNEILVMPTQQKI"
     gene            complement(5342..>6235)
                     /locus_tag="PLF20_08385"
     CDS             complement(5342..>6235)
                     /locus_tag="PLF20_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HOX81056.1"
                     /translation="AFTGYDIGGFGGDASPALYARWISLGALSPFCRTHSAYNTKSSE
                     PWSYGEDVEDIARNYINLRYRLLPYLYSQFYEASQTGMPVQRALAIEYAQDPRSLHTS
                     YQNQYSLGPSLLVAPCKSTDEYVKVFLPRGNWFNIYNDEFLNGDNEIIVESPLQRLPV
                     FVKAGSIIPVHPVRQHTAEDPGDTLELHLFYEKGLHEFIYYEDDGLTYNYKNGEFYKR
                     IIRFNGDRRELTLSSKEGSLPSHFHSLKIVLHGFEKLDSNFQKNITYQKISVLPDGFG
                     YDIKNPCITLTFDENEIKIKL"
BASE COUNT         1718 a         1214 c         1356 g         1947 t
ORIGIN      
        1 gcaaatgggt agcataactg tgacgcaaac agtgcggggt tacatgacgg acaataggcc
       61 tcgtttgctg tcaacgtgtt caattttcag gcccagtaca tcgctccgtc gtaaaccgca
      121 ggtgtatatc aacgacaaca tggtacgatg tttcaaattg gcagtacctg ttataatgac
      181 agctacctct tgtttactca aaaccttggg aagcaaatgc tcgcgttgcg gtcgttctaa
      241 tacttcgggg tcaaattgag tctttactat ttgcttaaaa aagagttttg ctccacttac
      301 ggtctggttt tgcgtggtat ttcccaaatt gttaggtatt aaatattcgt tgacaaaccg
      361 cacaaagtcc tcgtcagtaa tagattcggg tattttgggc agaacaaatc gcaaaaaagt
      421 ggtaagtgcc gatatatagt tttttacggt cgattccgaa tatcgtttat gaatcatcca
      481 ttgttcaaac tgtctaatct gatttttggt ggttggtggc aactctttgt cgtaaaggct
      541 gattttggtg gtttctgcca ggtttgtttt tctctttggt tcgtcaatgg tcacaatttg
      601 ggttatccgt ttgccaaaat attgcttaat ccggtcaaga ttatcggcat agttttgcat
      661 ctcccaccgg taagattttg gattccagcg actaaatttt atggtcttga caaaggcaat
      721 atctgctgtg tttttgggca tttttagcca tatattcttt gccgacacct cgaccacaat
      781 ctcatttctt gctttttgtg gttcctgcgg ttcagccttg gggggtattt cgggtgtagg
      841 ttgaacaatg gggctgtcgg gaaaaagctg gttaaatttt tccacgtcct cgttggtgtc
      901 cggaatatgc catgcttttt gcgactgact ccattgtgca ccgggtattt gtttaacccg
      961 ttcaatgcgt tctttatcgt acccaaactc aattctgatt ttttcagagc ctttgtgctg
     1021 aatcttttct atttttatgg ttgacatagg cttaatagtt gttatggttg acaaattttc
     1081 ataaaaactt tcgattgaca cacagacgaa taaaaatcca gtccatgtcc gaagtgcttt
     1141 acgtgctcga agcacaatcg tatattagcg cataattaat taaacttttg gtttatgaaa
     1201 cgaacatgcg gactggacgt gcacaaagat acaatttttt gtgcgacata tgatggtaag
     1261 aaacacggct ctgtgataga gtattcaacc ctgactacct ccatccgggc catgggcgca
     1321 gccttacttt cagaggaagt taaggaaatc gcgatggaga gtacgggaat gtactggata
     1381 cccgtttgga acattttgga agagatgggc tttaacctga cgctggttaa cccctatcta
     1441 ataaagcaga tgccaggaag gaaaagcgat gtgaaagacg cccaatggat tgcaacctta
     1501 ttgcataagg ggttattacg aagtagttta gttccctgca aggaaatacg agagttgcga
     1561 acctatagcc ggcggtatgt caaattgcag caacggaaaa catcagtact ccaggacatg
     1621 gaacgaaccc tggaggtgtg tagcattcgg atcaccagcc ttgtgagcaa catatctggg
     1681 aagagtgtta tgaaggtcat ccatgcactc atagaaggag aaaacgatcc tgaggttttt
     1741 gctgggtata tccatggtcg gatagtcaat aagcataaaa gaaaaacaat agtagaatct
     1801 ttaagaggac atgtcatctc gtagcatctt gttctattag aatatcgctg gaggaatatt
     1861 agctgctgtg taggcagaag gaacgctgca aggaaaagta caatcctcaa aagttaatca
     1921 tgtacgaacc ggggaaggtc caagcaaaaa tggattacca tcaaaaaggt tcaaacgctt
     1981 ggaaaacctt tttgaaaact aatgcgcaaa ggacggccat ctttatggag ggtaaaaccc
     2041 cgttttcgaa ttggccaatg caacttgtcc tgaaaaggac tttaagaaca tagacatgag
     2101 ttaatgttca gcatactggg ctggaccaaa ctcgcaggag aaactcgcaa caccaaaggt
     2161 gataattttt tggtactatt tataggagaa agatacctcg aaaattttaa aaaacaaaga
     2221 gattttaaaa aacggaggat ggtgattggt ggatggaggt tggtgattag gcgatgtcgg
     2281 ttatcgttgg atgattttta aggaggtcgt tcatattgaa gatgcctttg cgacctaaaa
     2341 gccattgtgc ggccatgatg gctccaatgg caaatccact gcggtcgaaa gcttcgtggg
     2401 tgagggttat cctgtcgtct ttcgaacgaa attccagtgt gtgaatgccc gtgatgtcat
     2461 cttcacgtat agaagtaata ggaatttgat gtggcagggc ttgctcgcct tctttgcatt
     2521 ttgtccattc atatttttgc ggcaagtttt tgattaatct ttcggccaat gcaatggctg
     2581 ttccgcttgg ggcatcgagt ttccgggtat gatggatttc ggtaatagca ggttcatatt
     2641 ggggataatc tttcataata tctgccaaaa tctcgcttaa gttcataaaa atattaaccc
     2701 ccaaattgaa gttaggcgca taaaacaagg ctccatgttt ttgtccaacg tagttgctta
     2761 tgaacgacaa ttggtcgtac catcccgttg ttccacaaac aatgggttta tgattatcga
     2821 tgctacgaat gatgtttcgt ggtgcttcgg caggaagact aaaatctatg acgacatcgc
     2881 actgtggaat gagttcggct ttatcgtccc aatcttttgc attgtcgtaa attccaataa
     2941 tttgatggcc tgattccagg gcagatcgtt cgacttcttt tcccattcgg ccatagcctg
     3001 aaattaatat tttcataatt tttcttgttt attgatgttc gggacgtaga agatcgttga
     3061 tagttttcac gggattgaag gtaatgagag gagtttcgac gaaaacggta agccattttg
     3121 ccatggctcc attccaaaga cccggatgtt ccaaggcttt caagtcgcgg tcgtcttttg
     3181 acttgataga gatgaagccc gtggaaggat cgacataatt gggcaaatga aatcgacgac
     3241 ctttataatc ggtgagacta caaaccagat cgacaggatt gaaatgagtg gattgggcca
     3301 ttatggcttt ttgcttcgga tcatttaaat tgatttgtga agattcaaca atctgtaaac
     3361 tgatttcgcc cgattgattt ttcaccagga aaggtccgcc cccaggttct ccaatgtttt
     3421 ttaccatgcc acaaaccctt acagggcgat cgagcttatc cttccaccat gcagctttcg
     3481 catgattgga catgtttttg tattcatcgg gtaaataaat aaataaagtc tcggaagcaa
     3541 atttttcgac ttcatttaat aactcattat ctacggctgt atgttcgagg tgtagcaaaa
     3601 aatcatcgac tcgattacgt atcttaagta aaaggcctcc tatgactttc ttatatttaa
     3661 tagtctcact tctgagcctg tcgccaataa tgttgtcgat atttttgata aaaacgattt
     3721 caccttccag ttcgttcagg ttttcgagca aggcgccatg accacctggt ctgaagagta
     3781 agcttccatc cttatttcgg aaaggttgat tgttcaaatc gacggcgatg gtatccgtag
     3841 atgacttctg gatggaatat tctattctaa agtgcacatc cagttttttt tcataaaaag
     3901 gggcttttct tgtgatgagt tcttcgaatt tttctttatg ctcgggcgaa accgtgaaat
     3961 gtattcttgc ctcatcattg tttttccttg cgtgttgtgc gccttctact aaatgttctt
     4021 ctatgcttgt acgtggccct tcgggatatt tgtgaaaaag aataagaccc ttaggtagat
     4081 ttccataatt gagtccccaa ggttcgagta cacatgaaat aagcaaattg taatctttct
     4141 tttctaaaac tttgtcgata gacgtgcctt tctgatgaaa aatgttttcc aactgctcgg
     4201 aaaatgcaaa atcgcgaatc cgatgaatga aagtataaac cgattgcaag cctctatcgc
     4261 tttctaattt tgccagtgca tcctccgaac catcatattg ttccctgaat tcgtagagat
     4321 gtttaaacat ccgcgaagct gcgccagatg caggtacaaa cttgagaata tccaatccat
     4381 gacgattctt ttcataataa ttttcaagtt cggaaatttg cgcatcatcg aacctgagaa
     4441 ttccatctcc aaccgttgca ggtcttacaa gttcagccca tggaaaccct tccctgaaat
     4501 attgaatctg tttttcaacg gtttctttgc taataccttt ggaggctaat tgttgtaaat
     4561 cgttgggtgt aaacatcgtt tgaactgttt ttaaattttt tgttgtgtgg gcatgacaag
     4621 aatttcgttc acattgactt gaggaggttg tgttagcgta taaatgactg cagctgcaat
     4681 atcactgggt tgcaaaggca taatgggagg attgtcttca ctgctggctc tgatgtcggg
     4741 atcgttgatt gtgctgggaa gattagtggc cgtaatacct ggttctatta gagtcattct
     4801 gatgttttcg gtcgatgtaa tttcctgcct gaatccttcc gaaaaagcat tgacggcaaa
     4861 ttttgtggca cagtaaaccg cacccccatc gaataaccaa cgacctgcag ttgacgaaat
     4921 attaacgata tggccataac cctgagaacg catatatggt agcacagctg ccgttccaaa
     4981 caatactccc ttcaaattca catcgatcat tctttcccac tcgtctaccc gcaaatttct
     5041 aataaatgat aagggcataa tcccggcatt gttgatgagt atatctattt tttgccagct
     5101 ttcgatgact ttatggataa attcattcca attgtcctta ttgctcacat ccatcggaaa
     5161 ggcaatagct tctttacctg attgtagaat ttccttttct actttataga gaaaatctaa
     5221 atgacgtgct gcaattgcca cttttgcacc ttctttggct aattcgaggg caatggcttt
     5281 accaataccc gatgatgcac ctgtaattat tgcaacctta gaatcgagtc tcatcattta
     5341 cttaaagttt tattttaatt tcgttttcat cgaaagtaag tgtaatgcat ggatttttga
     5401 tatcgtagcc aaatccatcg ggtaagactg aaattttttg ataagtaata tttttttgaa
     5461 agttggagtc gagtttttcg aatccgtgca aaacaatttt gagactatga aaatgagaag
     5521 gcaatgatcc ctctttggac gatagggtta attctcttct atcgccatta aaacggataa
     5581 tgcgtttgta aaattcacca tttttataat tgtaggttaa tccatcgtct tcgtaataaa
     5641 taaattcatg aagtcctttt tcgtagaaaa gatggagttc gagcgtatct ccgggatcct
     5701 cggccgtatg ctgacgtaca ggatgtactg gaattataga gcccgccttc acaaatacgg
     5761 gtagccgctg aagaggcgat tctacaatga tttcattatc accgtttaaa aattcatcat
     5821 tataaatatt gaaccagttg ccacgaggca gaaatacctt tacatattcg tcggtacttt
     5881 tgcaaggagc taccaaaaga gagggtccaa gagaatattg attctggtat gaggtatgca
     5941 aggagcgagg atcttgtgca tattcaatag ccagtgctct ttgaactggc ataccggttt
     6001 gtgatgcttc atagaactgg ctatacagat acggcaagag tcggtagcgc aagttgatat
     6061 aatttctggc aatatcttct acatcttctc catagctcca aggttcggaa gatttggtat
     6121 tataggcgct gtgagttctg caaaagggag agagagctcc taagctgatc catctggcat
     6181 aaagtgctgg cgaggcatca cctccaaacc cgccaatatc gtatcccgta aaagc
//
LOCUS       DAOORU010000140         6163 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_15521_length_6163_cov_5.297315, whole genome shotgun sequence.
ACCESSION   DAOORU010000140 DAOORU010000000
VERSION     DAOORU010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15521_length_6163_cov_5.297315"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1389)
                     /locus_tag="PLF20_08390"
     CDS             complement(<1..1389)
                     /locus_tag="PLF20_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOX81057.1"
                     /translation="MKVSHRLSIFRIVVAILSFTLITLAFFDYVSKLSFHHFRTILFL
                     QFVPSLLQFIQSPSIITAGFILVILLTLLFGRVYCSWICPLGILQDGLCKLKPHKRKR
                     RNRYQYYQTNKWLKYSILILTFVSILLGSTILLRFIDPYSLYGKMTYQLGSPVVVGVN
                     NLAFDILQQMGNYSLKPLPYPSFNLSAFVFSIGFLGILIFLVFWRGRVYCTEWCPVGT
                     LLGLVSKFSIFKLRVSESDCTACGLCARNCKAGCIDTKLKTIDFERCVACFNCLRTCS
                     AHAIIFTHHQSKIGESNLYHPDIQRRRILGMMITATAGLGLKSAMAQANIGTKAMLPN
                     PKPYPVTPPGSLSLEHFNKTCTACHLCINACPTQVLQPAMLQFGLIGLFQPYLDFHKN
                     FCNYDCVRCGEVCPTGAIKRLNSDDKKLVQIGQAHFFRENCVVYTEETACGACSEHCP
                     TKAVHMVSYKAGL"
     gene            complement(1389..2321)
                     /locus_tag="PLF20_08395"
     CDS             complement(1389..2321)
                     /locus_tag="PLF20_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005675936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOX81058.1"
                     /translation="MERREFMRKLCLGAVGGTLMVKGMRAQSKDLELLTPSFIPSSSG
                     YDLVAVKGGEPEVMFDKAIAAMGGMTQFVKAGQKVVVKPNIGWDVAPERAGNTNPQLV
                     SHIIKRCLEAGAAEVCIFDNPCDNWVKTYKTSGIEYAVKLAGGKMFPANEERFFKNVN
                     IQGKVLKTAAVHELILNSDVFINVPILKNHGSTLFTGSMKNLMGVVWDRRFWHRNNLH
                     QCIADFPKFKKPDLNVIDAYRVMFRNGPKGLSQADVKLMKSMIISRDIVAADSAASRL
                     IGLDPSEITHIVMANNSKIGTMDLSKLSIQRINI"
     gene            complement(2674..4011)
                     /locus_tag="PLF20_08400"
     CDS             complement(2674..4011)
                     /locus_tag="PLF20_08400"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOX81059.1"
                     /translation="MKKVLILGAGLIARPIVKHLLEQGYLVTVATRTKSKADEMIKKH
                     PNGTAIAWTVENEDMLESMVHENDLTVSLLPYAYHVLVAKACIKAKKHMVTTSYVKPE
                     MQALDEQVKEAGIIILNEMGLDPGIDHMSAMRIIDHIHQKGGKIEQFYSLCGALPAPE
                     AADNYFRYKFSWSPKGVVMAGNNDACFLMNGKIIEIPSKDLFKNSMKVCFPEVGMLEV
                     YPNRDSILYKEIYGIEEAKTMMRGTFRYPGWCVTIDTMKALNLISYDKYNFAGKTYAE
                     FVGMMIGIEDASGIRQKVADYLGIDVNNYALQAMQWLGLFDDKPMGRAIDSPFEITSD
                     LMISKMQLGDNEHDMVVMQHNFLAVYPDGKREVIRSRMLDYGTPTTDTSIARTVALPA
                     ALGVRMILEGKINIKGVYRPVLPEIYNPVLDGLEELGIKMNEEYGLPESEILL"
     gene            complement(4047..5366)
                     /locus_tag="PLF20_08405"
     CDS             complement(4047..5366)
                     /locus_tag="PLF20_08405"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysine ketoglutarate reductase
                     /saccharopine dehydrogenase family protein"
                     /protein_id="HOX81060.1"
                     /translation="MTQLRIGIRHEDKYAAERRAPLVPRHVAELIRKHGLEVYLQSSP
                     KRIFSDKEYEQAGAKVVSNLEDTPVIFGIKEIPVEVFEYGKTYIFFSHVIKGQPYNMG
                     MLRRMMELKCNLIDYERIVDDFGKRLIFFGHYAGLAGAINTLWTLGLRLKYFGFDTPF
                     LKIKQAYHYKDLNEACKEVSNVGREISRKGLPTELLPFTIGVTGYGNVSSGVQEILSL
                     LPGIEILPEDLLKLKDSGEFQNNLIYKVTFKEEHISTPNDPYKAFDLDEYYSLPENYH
                     GVFEQYLPHLSVLINGIYWDSRYPRLITRQYLKENFSGAKQPKLIVVGDITCDPEGSI
                     EATLKGTTIEDPIFVYHPQTHSMTSGYEGEGLQIMAVDILPSELPRDSSLGFSNVLKN
                     YVYDIAKANYSAASAGEIQLPAPIKRALILHKGELTPDYKYIQKYLE"
BASE COUNT         1954 a         1279 c         1072 g         1858 t
ORIGIN      
        1 aaggcctgcc ttataagaca ccatgtgaac agcctttgtg ggacaatgtt ctgaacaagc
       61 tccacaggca gtttcctcgg tataaaccac acagttctct ctgaaaaaat gagcttgccc
      121 tatctgaacc agttttttgt catctgaatt taatcttttg atagctccag tagggcaaac
      181 ctctccacaa cgaacacaat cgtaattaca aaaattttta tgaaaatcga gatagggctg
      241 gaaaagcccg attaatccaa attgcagcat agcaggctgt aaaacttgtg ttggacaagc
      301 atttatacat aaatgacagg ccgtacaggt tttgttgaaa tgttcgagag agagagaacc
      361 cggtggagta acaggatagg gtttaggatt tggcagcatt gcctttgtac cgatatttgc
      421 ctgagccatg gcagatttca gtcctaaacc tgcagtggcg gtaatcatca tacccaaaat
      481 ccttctacgc tgtatatcag gatgatataa attagattca ccgatttttg attggtgatg
      541 cgtaaaaata atggcatgag cagaacaagt gcgtaaacag ttgaagcatg ccacgcatct
      601 ttcgaaatcg atcgttttta attttgtatc aatacatccg gctttgcaat ttcgggcaca
      661 aagtccacat gcggtgcagt cggattcaga aacgcggagt ttgaaaatcg aaaatttcga
      721 aaccaaaccc aacaaagttc caacaggaca ccattccgta caatacactc tgcccctcca
      781 aaaaaccaaa aaaattaata ttcctaaaaa tcctattgaa aaaacaaagg ccgatagatt
      841 gaaagaagga taaggcaatg gtttaagcga ataattaccc atttgttgaa ggatatcgaa
      901 agccaaattg ttgacgccca ccaccacagg actccccaat tgataggtca ttttcccata
      961 aagagaataa ggatcgataa atcgcaataa aattgtagaa cctaacagaa tagacacgaa
     1021 agtcaaaata agtatgctgt attttagcca tttattcgtt tgataatact gatacctgtt
     1081 ccttcttttc cttttgtgtg gctttagctt gcataatcca tcctgaagta ttcccaatgg
     1141 acaaatccat gaacaataca ctctcccgaa cagtaaagta agaagaatga ccagaataaa
     1201 acccgcggta ataatcgaag gtgattggat aaattgtaaa agtgatggaa cgaattgcag
     1261 aaaaagaatc gtacgaaaat ggtgaaacga caatttgctt acatagtcga aaaaggccaa
     1321 agtaatcagt gtaaacgaca atatggctac gacaatgcga aatatactca atctatgcga
     1381 tactttcatc agatattgat acgttggata ctgagtttcg acaaatccat cgttcctatt
     1441 ttgctgttat tggccataac gatatgggta atttcgcttg ggtcaaggcc aatcaagcgg
     1501 gaagcagctg agtcggcagc cacaatatct cgagaaatga tcatcgattt cattagtttt
     1561 acatcggctt gagataaacc ttttggacca ttacgaaaca taactcggta agcatctatt
     1621 acgttcagat caggtttttt aaattttgga aaatctgcta tacactgatg aagattgtta
     1681 cgatgccaga aacgcctgtc ccaaacaact cccattaagt ttttcataga accagtaaac
     1741 aaggtagagc catggttttt aaggatagga acattgatga acacatcgct gttaaggatg
     1801 agctcatgaa ccgcagccgt ttttaatact ttcccctgga tattgacatt tttaaaaaat
     1861 ctttcttcat tggctggaaa cattttacct ccagctaatt ttactgcgta ttcaattcca
     1921 ctggttttat aagtttttac ccaattatca cagggattgt cgaaaatgca aacctcagca
     1981 gcaccagctt ccagacaacg ctttatgata tggcttacca gttgtggatt ggtattacct
     2041 gccctttcag gagcaacatc ccaacctata tttggcttca cgacaacttt ctgtccagct
     2101 ttgacaaatt gggtcattcc ccccatggca gctattgctt tatcgaacat cacttctggc
     2161 tcgcctcctt taacggcaac caaatcatat cccgaggaag aaggaataaa agagggagtt
     2221 aataattcta agtccttact ctgtgctctc attcccttta ccatcaaagt tccacctact
     2281 gcgcctaaac aaagctttct catgaattcc ctgcgttcca taatttaatc gactgatttt
     2341 taatattttt ccttatattg atatttcatc aattatctac aaaccaacaa ttttattaca
     2401 aatgtaaaag attctcacta aaaattgagg caattcttat attttcaaag atattttcta
     2461 tgatattaag caaatggctt gaaaagaaac gttatgactc ttaaaaaccc caatcgaaaa
     2521 ctcaaggaag gtttttttca gtttggtcat acgggaattt ggacatgaaa agatgataat
     2581 gcgagctttc agagaaactt aaaaaagtat gaatacattt tgggctggca aaaaagtctt
     2641 gccagcccaa aactcaaatt tcatcgattg gttttaaaga agaatctcac tttccgggag
     2701 gccatattct tcattcatct tgattccaag ttcttctaat ccatcgagaa ccggattata
     2761 aatttctggt aatacaggtc gataaacgcc tttaatgttt attttccctt ctaaaatcat
     2821 tctaacgcct aaagcagcag gtaaggctac ggtgcgggca atgctcgtat cggttgtcgg
     2881 tgttccataa tcgagcatgc gcgagcggat tacttcgcgt tttccatcag gataaaccgc
     2941 caaaaaattg tgttgcatca ccaccatatc gtgctcatta tcgcccaatt gcatcttcga
     3001 aatcatcaga tcggaagtaa tttcaaaagg agaatcgatt gcacgtccca taggtttatc
     3061 atcgaacagg ccaagccatt gcatggcttg caaagcataa ttatttacat caattcccag
     3121 ataatcggcc accttttgac gaatgccaga agcatcttcg attcctatca tcattccaac
     3181 aaattcagca taagtttttc cagcgaaatt gtatttatcg tacgaaatca aattcaatgc
     3241 tttcatggta tcaatggtta cacaccaccc tggatatcta aaagttcccc tcatcatagt
     3301 cttggcctct tcgattccat aaatttcttt atacaaaatc gagtcgcgat tgggataaac
     3361 ctcaagcatt ccaacttcgg ggaaacatac tttcattgaa ttcttaaaca gatctttcga
     3421 tggaatttcg ataatttttc cattcatcaa aaaacaggca tcattatttc ctgccataac
     3481 tactccttta gggctccatg agaatttata tctgaagtaa ttatcggctg cttcaggtgc
     3541 aggtaaagct ccgcaaagac tataaaattg ttctattttt cctccctttt gatggatatg
     3601 atcgataatt ctcattgccg acatgtgatc gatacctgga tcgaggccca tctcgttgag
     3661 aatgataatg ccagcttctt ttacctgttc atcgagcgct tgcatttcgg gtttgacata
     3721 ggaggtggtt accatgtgtt ttttggcctt gatacaggct ttggcaacca atacatgata
     3781 agcatagggt aataagctta cggtaagatc attttcgtga accatgctct cgagcatatc
     3841 ttcattttcg acagtccatg cgatggccgt gccattagga tgcttcttga tcatctcatc
     3901 ggctttcgac tttgtacgtg tagccactgt aaccaaatag ccctgttcca acagatgctt
     3961 tacaataggt cttgctatta agccggctcc aagaattaat actttcttca tgtgaatcga
     4021 tttattggta ataaaatgaa aaattcttat tccaggtatt tttgaatata tttataatca
     4081 ggtgttagct ctcctttatg gaggatcaag gcacgtttta taggcgcagg caattgaatt
     4141 tcacccgcgc tcgcggccga ataattggcc ttagcaatgt catagacata attttttaaa
     4201 acattgctaa aacccaaaga ggaatcacgt ggtaactcac ttggcagaat atcgaccgcc
     4261 attatttgta aaccttcccc ctcgtaacca ctggtcatgc tatgggtttg cggatgataa
     4321 acaaaaatgg ggtcctcgat ggtagtgcct tttagtgtag cttcgatgga gccttcaggg
     4381 tcacaggtaa tatctccaac aacgataagt ttaggttgtt tcgctcctga aaagttctcc
     4441 tttagatatt gcctggtaat taaacgtgga taacgcgaat cccagtatat tccattgatt
     4501 agaacagata gatgaggcag atactgttca aaaacgccat gataattttc aggcaaggaa
     4561 taatattcat ccagatcgaa agccttataa ggatcattcg gagtacttat gtgttcttcc
     4621 ttgaatgtca ctttataaat cagattattc tggaattcac cagaatcctt taatttgagc
     4681 aaatcttcgg gcaaaatttc tatccccggt aatagcgata atatttcctg aacaccactc
     4741 gatacattgc cataaccagt aactccaatg gtaaagggga gcaattcggt tggcaatcct
     4801 ttcctcgaaa tctcacgtcc tacattggaa acctccttgc aggcttcgtt caaatccttg
     4861 tagtgatacg cctgttttat ttttagaaaa ggtgtatcga aaccaaagta tttcaatcgt
     4921 agacctaagg tccacaaggt attaatggca ccagcaagtc ctgcatagtg gccaaaaaaa
     4981 atcaaacgct tgccaaaatc atcaacaata cgttcataat caatcagatt acacttcaat
     5041 tccatcatac ggcgaagcat tcccatgtta taaggttgcc ctttaataac atgtgagaaa
     5101 aaaatataag tcttcccata ttcgaaaact tccacgggta tctctttaat cccgaaaatt
     5161 acgggagtat cttctaaatt acttacaacc ttagcacccg cttgctcata ttccttatcg
     5221 ctgaatattc gtttgggaga agactgaaga taaacctcca atccatgttt cctgattaat
     5281 tccgccacat gccgtggaac caaaggagct ctcctttcgg ccgcatattt atcttcgtgg
     5341 cgaattccaa ttctaagttg tgtcatatca ttcaataggt tatcaattta taatttcctt
     5401 ccattatgtc tgcaaacata cgaaaataaa aactttcggc aaaatttctc tgtttttaaa
     5461 attttcccgc ccaattatat taaaaactca tcaattaaat aataatcaat tgctaaccaa
     5521 taatttgaaa tagtaataga tggctttgtc atcaaagtaa aagtaaatac tgagagctat
     5581 tttgaaatcg actcgaaggc aaaaagggaa gtaaaagcca ttcgaaataa tttcgatatt
     5641 cctgtaattt tttcaaaaaa ccgaatgaga tacccttaaa aagaaccaca gtagaatagc
     5701 ttgattcgac aatttatcca ttaaaacctt acaataccgg agcgataagg gagcttaaca
     5761 ttacaaagct catatcgaga ctattgtaaa gaagtagtcg tctccataag cccttaatga
     5821 ccattcgaaa acaatgaagg caatggttaa gcaaggcgaa accctattag aagccactgc
     5881 gcaaatggaa ttttatgttt tccttaattt tagagtgcat tcgcctttct ttaaatactt
     5941 ttatctgctt ctactcattc taaggcataa aagggttcat tccgtcctgt agatatatta
     6001 agtaatgatt caaattatta caattcattt cccatcaaag tttataagat ttttaatcac
     6061 tattaaccga caaaattcta aaaagctcct aaacattttt tatataaaac ttattcagtt
     6121 agttatacat tagcttttcc ttctttaaaa aacctacccc tta
//
LOCUS       DAOORU010000141         6161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_15528_length_6161_cov_4.756469, whole genome shotgun sequence.
ACCESSION   DAOORU010000141 DAOORU010000000
VERSION     DAOORU010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15528_length_6161_cov_4.756469"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PLF20_08410"
     CDS             complement(<1..882)
                     /locus_tag="PLF20_08410"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HOX81061.1"
                     /translation="MYNTLKLYRFNILIPLRANILRNWDYIRKWRLRKISDRQLVNIL
                     GVIVGFLSGLSAIILKNTVYYTHAFITSDFGFGNNSFLLFFSPLIGILLTVLFIRYFI
                     KDDISHGISKVLYAISKNNSLLKPHNTFSSMIASTLTIAFGGSVGAEAPIVLTGASIG
                     SNLGRIAKVSYKHRTLLIGCGAAGAIAGIFKAPIAGIVFTIEVIMIDLTMATLIPLLL
                     SAITASVISFFFLGDDVLFSFTLESSFDLSRLPFYAILGLFCGFVSLYFTRASDYTES
                     KLQRIGNPFHRLLVGGVI"
     gene            complement(1318..2772)
                     /locus_tag="PLF20_08415"
     CDS             complement(1318..2772)
                     /locus_tag="PLF20_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOX81062.1"
                     /translation="MEQIINVIEYSQQFSILEISYIIASVLYIVGLKRLSSPNTARSG
                     NLWAAAGMFLAILFTILFHKVKQEDGTYEGIHNIPLIVVAFAVGGTIGWTAARKVKMT
                     AMPQMVSLFNGMGGACAALISLMEFPHVPDVLVEHHGMLNGYTLSVILGLMIGSVSFA
                     GSMIAFGKLDGKIGDIKASWLKYLNMIILASVLIFAIYILQLDHKPSMGLVYLFAFLA
                     LFYGVSFVMPIGGADMPVVISLLNSFTGVAAAMGGFLYGNRAMLTGGILVGSAGTILT
                     ILMCKAMNRSIINVIAGAFGGKSKEGAADIDRTVREINLSDAAILLCYSQKVYIVPGY
                     GLAVAQAQHLCHELDLLLSSKGVEVKYGIHPVAGRMPGHMNVLLAEADVPYEKLVEAE
                     DANNEMPNTDVVIVIGANDVVNPAAEEDPSSPIYGMPVIRVWEAKNVIVMKRSMAKGY
                     AAIENNLFYHPRTRMLFGDAKVSLQKLIAEIKSL"
     gene            complement(2785..3096)
                     /locus_tag="PLF20_08420"
     CDS             complement(2785..3096)
                     /locus_tag="PLF20_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HOX81063.1"
                     /translation="MDGVLRFIYEYKDAIFIIILSIFLGIEVISQVPAVLHTPLMSGA
                     NAIHGVVIIGSIIVMGHAHETLPLILGFIAVILGTLNVVGGFVVTDRMLEMFKKKKDG
                     K"
     gene            complement(3119..4246)
                     /locus_tag="PLF20_08425"
     CDS             complement(3119..4246)
                     /locus_tag="PLF20_08425"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOX81064.1"
                     /translation="MRVGILKEQPPENRVVLLPEGVATLVKLQIEVLVEQGAGIRAYA
                     DDAMYEAAGAKILTREDVIGLSEVILMINAISSTDIALLKRGQVIIGALNPYFEKDYV
                     KALAAAGITAFSMDVIPRTTRAQTMDILSSMSTVAGYKAVLKAANELPKFFPMLMTAA
                     GTISPAKVLILGAGVAGLQAIATSRKLGAVVEAFDVRTAAKEEVLGLGAKFVEVEGAI
                     EDKSAGGYAVEQTEEFKKRQAQAIHDHAIKSDVIIGTAQIPGKRAPLLLRTETVEAMR
                     PGSVIIDLAASTGGNCELTQNNKTIVHHGIKIIGNSFFPSEIPTDASRMLGKNYINFI
                     KLLITNEGNLNLNWDDDIVKGTLVTHEGQIVHERVKSLINN"
     gene            4679..5239
                     /locus_tag="PLF20_08430"
     CDS             4679..5239
                     /locus_tag="PLF20_08430"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HOX81065.1"
                     /translation="MDFIYIIFLAISLSFDTFAVAISCGLCLPQISLRNALKISLPLA
                     FTQAVMPLIGWLIGSSFEQTLKSYDHWIAFLLLSFLGVRMIVSSFRGKKPPKPFNPLN
                     LPTIMGLSVATSIDALIVGFSLSLIEANLIFAVFSIGFITLIAAMLGISIGKKTSPLL
                     GKKMGAVGGVILIVIGLKILLSHILV"
     gene            complement(5503..5664)
                     /locus_tag="PLF20_08435"
     CDS             complement(5503..5664)
                     /locus_tag="PLF20_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81066.1"
                     /translation="MKIQAELVLNSLLYHPAEALIIGEPRPDASVFSEENEQGMASAL
                     LKPNRSMTY"
     gene            complement(5945..>6161)
                     /locus_tag="PLF20_08440"
     CDS             complement(5945..>6161)
                     /locus_tag="PLF20_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81067.1"
                     /translation="YSSWYINPPEEGIATVDLRNRHFYNVSYIPMIMKFLVLSQYLLV
                     YGRERKRNYISILMIRRWIVCAVLRLS"
BASE COUNT         1932 a         1335 c         1121 g         1773 t
ORIGIN      
        1 tataacacct cccactaaaa gtcgatgaaa aggattaccg atgcgctgca gcttggattc
       61 cgtataatcg gaagccctcg taaaatataa ggaaacaaag ccacaaaata agcccaatat
      121 agcataaaat ggaagacgtg agagatcgaa cgaactttcg agggtaaatg aaaacaaaac
      181 atcatcccca agaaagaaaa aagaaatgac agaggccgtg atagccgata aaagaagagg
      241 tattaaggta gccatagtca ggtcgatcat gataacctcg atggtaaaaa caataccagc
      301 gatgggggcc ttaaaaatac cggctatagc tccagccgct ccgcatccaa tcagtaaggt
      361 tcgatgtttg tatgaaacct ttgcaatccg ccccaaattg cttcctatag aagcacctgt
      421 gagcactatg ggagcctcag cccctaccga acctccaaag gcaatcgtta aagtgctggc
      481 tatcatcgaa gaaaaagtat tatgaggctt caacaaactg ttattcttcg aaatagcata
      541 taacacttta cttattccat gtgatatatc atcttttata aaataacgaa taaaaagcac
      601 tgtaagcaat atccctatta agggcgaaaa gaacaatagg aagctgttgt taccaaagcc
      661 aaaatcactc gtaataaaag catgcgtata ataaaccgta tttttcagaa taatagcact
      721 caacccactc aaaaagccta caataactcc aagaatattg accaactgac ggtcagaaat
      781 ttttctcagc cgccatttcc tgatgtaatc ccaattcctt aaaatattag cccttaatgg
      841 aataaggata ttgaacctgt ataattttaa tgtattatac ataattttgg taggaataat
      901 cccattttat taacgagaat ctgattgcat ttcaattttc catgaaattc gctaggcaaa
      961 aataaaaact tatattttgc aatcctttaa tattttacat ttaaggaacc acatcgaaaa
     1021 atactttcag attaaaaatt ttaaacgcat ttcgagccag tgtcttattt tatcttcttc
     1081 attttgccat atttatttcg ctttgtatcc ttagaactcc tgactctata attgcatatc
     1141 aatcccgtta gttatgacgg gacaaaagta gcatcgaatt tcgacgaatg gatataataa
     1201 ataatcattg aaactgaatt tacgcaaatc ggagccatta tacttgttat ggaaggagta
     1261 tatcgagagg cgaaaagtcg gcccacatgg ccgacttttc aatccaaagc gtaaagatta
     1321 cagactttta atttcagcga tgagtttttg caagcttact ttggcatcac caaaaagcat
     1381 tcgagtgcga ggatgataaa aaagattatt ttcaatagcg gcatatcctt ttgccatcga
     1441 acgtttcata acaatgacat tttttgcttc ccaaaccctg attacaggca taccataaat
     1501 agggctcgaa ggatcttctt cggctgcggg atttacaaca tcattagctc ctattacaat
     1561 taccacatcg gtattaggca tttcgttgtt ggcatcttcg gcctcgacta atttttcgta
     1621 aggaacatct gcctcggcca gtaaaacgtt catgtgcccg ggcatacgtc cagcaacggg
     1681 atgaattcca tatttcactt ccaccccctt actagataac aaaagatcca gttcatgaca
     1741 gagatgctga gcctgagcaa cggccagtcc ataacccgga acgatatata ccttttggga
     1801 atagcaaaga agaatagccg catcacttaa gttgatttct ctaacggttc tatcgatgtc
     1861 tgcagcgcct tctttgcttt tgcctccgaa agcccccgca atgacattta ttatagaacg
     1921 attcatagcc ttgcacatta aaattgtgag aatggtacca gccgatccaa cgagtatacc
     1981 accggtgagc atggctcggt tgccgtaaag aaatcctccc atggccgcag caacccccgt
     2041 aaaagaattc aaaagagaaa taactactgg catatcggct ccaccaatgg gcatgacgaa
     2101 actaacccca tagaacaaag ccaaaaatgc aaatagataa accaaaccca tcgaaggttt
     2161 atggtcgagc tgaaggatat aaatagcaaa aatgagcaca ctggccagta taatcatgtt
     2221 tagatatttc agccagcttg ccttgatgtc gcctattttc ccgtcgagtt ttccaaatgc
     2281 aatcatacta cctgcaaacg aaactgagcc aatcattaat ccaagaatta ccgacagggt
     2341 gtacccattg agcataccat gatgttcgac caatacgtca ggcacatgcg gaaattccat
     2401 aagagaaatc aatgcggcgc aagctcctcc cattccattg aacaatgaaa ccatttgcgg
     2461 cattgccgtc atctttactt ttctggcagc cgtccaccca attgtacctc ctacggcaaa
     2521 tgcaaccaca attaaaggaa tattgtgaat cccttcgtaa gtcccatcct cctgttttac
     2581 cttatgaaac aaaatggtga acaagatggc cagaaacatg ccagcggcag cccataaatt
     2641 tccacttcga gccgtattag gactgctcag acgctttaat ccgacaatgt ataatacgga
     2701 tgcaataata taagaaatct cgagaatcga aaattgctgc gaatactcaa tgacatttat
     2761 aatttgttcc atggcctacc tccgttattt cccatctttt ttctttttaa acatttcgag
     2821 catgcgatca gtgactacaa aaccgccgac gacgttcaaa gtaccaagaa ttacggcaat
     2881 aaatccgagg attaagggga gggtttcgtg agcatgtccc attacaatga tagaacctat
     2941 gatcaccaca ccatgaatgg catttgctcc cgacatcaat ggagtatgca aaacggcagg
     3001 aacctgcgat ataacttcaa ttccaagaaa gatggaaaga atgatgataa aaatcgcatc
     3061 cttatattca taaataaatc tcaatacacc atccataaaa tttattgtta agtgttaatt
     3121 aattattgat aagggattta actctctcat gaacaatttg accctcatgg gttaccagcg
     3181 tgcccttcac gatatcatca tcccaattga ggttgagatt accttcattt gtaatcaaca
     3241 atttgataaa gttgatgtag tttttaccga gcattcgtga agcatcggtt ggtatttcgc
     3301 tggggaaaaa ggaattgcca atgattttaa ttccgtggtg cacaatggtt ttattatttt
     3361 gggtcaattc gcagttgcca ccggtggaag ctgccaggtc gataatcacg cttcccgggc
     3421 gcatcgcttc gactgtttcg gttctcaaca aaagaggagc ccgtttgcca ggtatctgag
     3481 ctgtgccaat gattacatcg gattttatag catggtcatg aatagcttga gcctgtcgct
     3541 tcttaaattc ttcggtttgc tcaacagcat aacctccggc agatttgtct tctatggcgc
     3601 cttctacttc cacgaacttc gcacccaagc caagaacttc ttccttagct gccgttcgta
     3661 catcgaaggc ttccactacg gcgcccagct tacgtgaagt agctatagct tgcaatccgg
     3721 ccaccccagc acctaaaatc aataccttgg ctggtgaaat agtacctgca gccgtcatca
     3781 gcatggggaa aaacttcggt aattcatttg cagccttcaa aactgcctta taaccagcca
     3841 cagttgacat ggacgatagt atatccatgg tttgagccct tgtggtgcgt ggaatcacat
     3901 ccatgctaaa agcagtaatt ccagcggcag caagagcctt cacataatct ttctcgaagt
     3961 agggatttaa agcgcctata atgacctgcc ctctttttaa aagagcaata tcggttgatg
     4021 aaatcgcatt gatcatcaat attacttcgg aaagtccaat gacgtcttcg cgtgtaagaa
     4081 tctttgctcc tgccgcttca tacatggcat catccgcata agcacgtatt ccggctccct
     4141 gctctactaa aacctcaatc tgaagcttta ctaaagtggc aaccccttca ggcaaaagca
     4201 ctaccctgtt ttctggtggt tgttccttaa gaattcctac tctcatagaa tgaatattgt
     4261 tgaagaaaca aaagtataga atttcgtttt tgtgcatatc ctttaatccg aataaagtgt
     4321 gagttgtttc attcattttt tagccaatcg atgttttggc ttccatgaat ttttgataaa
     4381 taagatcctt gctatcatca agatgacctg aattcagaaa aaatcatata aaaatccatc
     4441 gaaaatatat actgaattac ttgatacaaa atatctggta aaaatcgaag caatgggaaa
     4501 tcgttggatg tataaagctt atagatgcac caggcgagaa agaatcaata aaatcatcaa
     4561 gaatatacaa tataccttgt gatacttctt atctaggcaa aatcaccact ttacatccta
     4621 ttctcaattt ttaaagatag agggttatac cctattttta cgagcctaaa ttctgagaat
     4681 ggattttatc tatataattt ttctggccat tagcctttcc tttgatacat tcgctgtagc
     4741 catatcctgt gggctatgtt taccacaaat ctcccttcgg aatgccctga agattagctt
     4801 gcctcttgct tttactcaag ctgtcatgcc tttgattgga tggttgattg gttcatcttt
     4861 cgagcaaact ctaaaaagtt atgatcactg gatcgccttc ctattgttga gctttttagg
     4921 agtaagaatg atcgtttcaa gttttagggg gaaaaaaccg ccaaaacctt tcaatcctct
     4981 caatctgcca actatcatgg ggctttcggt tgccacgagt attgatgcat tgattgtagg
     5041 attcagcttg tcattgatcg aagctaatct aatatttgct gttttttcga tagggttcat
     5101 aactctcata gcagctatgc tcgggatttc aatagggaaa aagaccagtc cgcttctcgg
     5161 gaaaaaaatg ggagcagtgg gaggtgttat tctaatagtc attggactaa aaatacttct
     5221 ttcgcatatt cttgtctagt taaaacaaat acttcaagtt ataatcccat aattcacacc
     5281 gtatctatcg agacgaaaca aaatcgacaa ttgaggatta atgcgacaga tgatccagaa
     5341 attttcatca agtgggcaag gcaaacgatc atgcaagcga tttttctctc ccataccaga
     5401 attctattct aaaagaggta aacaaggaat cttctctaaa acgctattgc tggaatttca
     5461 attgtcgtat ttttgtgtaa agattttcag ataagtgtaa acctaatagg tcatagacct
     5521 gttaggtttt aacagtgccg aagccattcc ttgttcattc tcctccgaga aaacagaggc
     5581 atctggtctg ggttctccaa tgataagggc ctccgctggg tgatatagca aactgttcaa
     5641 aacaagctca gcctgaattt tcatctcatt caattagcct gtaaggtatt gcctgggtat
     5701 tgggctcaaa ttcatttgca taacgatgag ttttctaaat aaaccctaac ctgcgaaaag
     5761 atttattcaa gtaaaaaaag tatttcgacc gaagtttagc cagatcaggg aatgagttca
     5821 aatacgggtc ggcctttttt aatcttctta accctgcatt tcactggatt tccaaaaggc
     5881 attgtaatat atttcgagtt tcccaacgaa atttcatatt catttccaag tccatcattt
     5941 actatcatga tagtcttagg accgcgcata caatccatct tcgaatcata agaatcgaaa
     6001 tatagttcct tttcctttcc cggccataaa ccagcaggta ctggctcaaa acgaggaatt
     6061 tcataatcat cggtatatac gaaacattgt aaaaatggcg atttctcaaa tccacagtgg
     6121 ctatcccctc ctcaggaggg tttatatacc atgaagaata t
//
LOCUS       DAOORU010000142         6160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_15533_length_6160_cov_4.074365, whole genome shotgun sequence.
ACCESSION   DAOORU010000142 DAOORU010000000
VERSION     DAOORU010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6160
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15533_length_6160_cov_4.074365"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..1002
                     /locus_tag="PLF20_08445"
     CDS             430..1002
                     /locus_tag="PLF20_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOX81068.1"
                     /translation="MTLKIEVVLPKEDDKRLLNLLAQEKTKNYGFELLVKTYQERIYS
                     IVRRMVIRHEDANDIVQDVFVKVWQNLDNFRQESSLYTWIYRIAVNESLRHLKSRKVK
                     IFSSDVDVQKHLEQLIDNPLHFNGDQLEAKLQKAILKLPPKQRLVFNMKYYEDLTYEQ
                     MSAILGTSVGALKASYHLAIKKLEHYLDIY"
     gene            1052..1573
                     /locus_tag="PLF20_08450"
     CDS             1052..1573
                     /locus_tag="PLF20_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81069.1"
                     /translation="MKTKKEIDREISEKRLESRFGKKNSFPVPEGYFADLPQNIIQKI
                     KSEQTFTLSFDHSPRTQRRWLIPAITIAVAAFIGAFFLFKPLTTQVNSIDKQVVSKGD
                     IQNDASDYYYISHILYNLPYDHLLNAINEEDDTYLDSVIYSRSLIPDGTLKEEEVIDY
                     LYYHMSIYDITQF"
     gene            1593..2072
                     /locus_tag="PLF20_08455"
     CDS             1593..2072
                     /locus_tag="PLF20_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81070.1"
                     /translation="MMKILGCGLLNLAFFAVPIITLAQPPQDNPDKWEQIKAQKIAYL
                     SAKLELKPSESQKFWPVYNEYSEKRDKLLNEIYPKLSKQELDRLMMLSDKEAAQRMDE
                     YFIKQTQLLDLEKKYNACFRKILPDSKVFRLYMAEMMFKKELLDDMRRSKHGPKEEY"
     gene            complement(2158..3081)
                     /locus_tag="PLF20_08460"
     CDS             complement(2158..3081)
                     /locus_tag="PLF20_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81071.1"
                     /translation="MKRTLLYLFLPVLVNLYSNQSIKAQKVHPNQDTLLAYWQIMLRG
                     ESDSVQRVASLKVKEILSQSFTTTQCLSLKLPKEIRLSALMPTDSAFLLITWNIPLST
                     GGYFYDGLLVAKNTPDSKLSVYPLKDKSDEIKNPETLITRPENWYGALYYQLLEFKSE
                     KGKFYVLLGWDGGNGRIQRKIIEVLSFDDDHLPRFGDKIFDQSPKTRLIFEYASGSYF
                     VLRYEKQHYRKKVWYKRKPVIKEEAMIVMNRLVYEPTVGANVAAANILDAYLFSDGKL
                     ILLKDIDARNPEEQNRPSPKNPPSQGLIPPD"
     gene            3162..4226
                     /locus_tag="PLF20_08465"
     CDS             3162..4226
                     /locus_tag="PLF20_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81072.1"
                     /translation="MTKAERLFILKRLGNLLRWITLTGVYFTPSDEEIELSKKLQRII
                     DENHHFNLWFDPFHVKYSLSALGLMLDETRLENWANRYIIPEDRINRKTIAVVCAGNI
                     PAVGFHDFLCVLVSGHNILVKLSSDDLLLLPWFSEFLHNTNPEFKNIITITEGTISGF
                     DAVIATGSNNTSRYFEYYFGKYPHIFRRNMNSIAILDGQESPQELSGLVDDILIYYGR
                     GCRNVSFLLVPENYDFSPLLDAFRKYSHYAYHSKFYNNYEYNKAVMLVNRIPFYDIGF
                     MLLTSHPSIQSPLAVIHYQTYKKSDEIYSFLHFNRDKIQRNVSHIQGIPIKVDFGKSQ
                     QPALDDYADGVDTMKFLENL"
     gene            4300..4566
                     /locus_tag="PLF20_08470"
     CDS             4300..4566
                     /locus_tag="PLF20_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOX81073.1"
                     /translation="MKKDIHPSDYRLVVFKDMSNDYAFLCRSTAKSKETIIWEDGKEY
                     PLVKVEISHTSHPFFTGKLTLVDTAGRVDKFKSRYQKHYQSKQK"
     gene            4895..6085
                     /locus_tag="PLF20_08475"
     CDS             4895..6085
                     /locus_tag="PLF20_08475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOX81074.1"
                     /translation="MNYILFDDYSRNNLLPLTFLRPVADLLTGILTIRQKWEYYLGDS
                     TSSLTEDYLIEKYPLYKGKNNILINGSVLPSDELIKAIRGLQPNQALINKDAIIALHV
                     PLENLDKIGEGDTEGIEEIDTDIDIIKINYLWDLFLNNAKQIEADYAMLTVGSKSIEP
                     SKSNHIIAPENIFIEEGALVENCNLNATDGPIYIGHNAQIWDGASLRGPIAVGEHAIV
                     KMNAIIYQATTIGSYSKVGGEVENSVIMAYSNKPHSGYLGHSIVGQWCNIAAGTNVAN
                     LNNTYKTIKMWNYPQSRFIDTGLQFCGLVLGDHSKTGINTTFNTGSVVGISSNVFGAG
                     YQRNFVPSFVWGGPSTGYSGYDFEKAIETAKEVYKRRGIELTDVDFKILQHVYEITKN
                     NIRL"
BASE COUNT         1924 a         1123 c         1191 g         1922 t
ORIGIN      
        1 ccccgctcca tctcctcgcg gtccatctgg tgttggaaaa aggtgagtcc tccgggttta
       61 tattgtgcaa taagctgatc gatcgattta tagtaatctt cgtctttatt actataaacc
      121 cttatgctca tcaattgagc tatcctttgt tcggttgtca agctcctata cactgaatca
      181 atccatctaa gagaatcgtg ctgcaagaca aattccgagg agaaatcttt actctctgac
      241 tggaacgata ttaaaagcaa aaaaaataga atgatctttg aaaaaacttt attgatcgaa
      301 ttatttttca taagaagcag attgaataat ttcaacctcg gcaaaagtaa tgaattagtt
      361 ttgttgcata cttttaccgt aatttggccg ttttgaaaat aagtatcaag aaggatgaaa
      421 taatcaaatt tgaccttaaa aatagaagta gttttgccaa aggaggatga taaacgactt
      481 ttgaatctgt tagctcagga aaaaaccaaa aattatggtt ttgagctctt agtaaaaacc
      541 tatcaggaac ggatatactc tatcgtcaga cgcatggtca taagacatga agatgccaat
      601 gatattgttc aggatgtttt cgtaaaggtt tggcaaaatt tggacaactt cagacaggaa
      661 tcatctttat atacatggat ttaccgtatt gcggtgaatg aatcattgag gcacttaaaa
      721 agcaggaaag tcaagatttt ttcgtccgat gtggatgtcc agaaacacct cgagcaactg
      781 attgataatc cattacattt taatggcgat caactcgagg ccaagcttca gaaggccatt
      841 ctaaaactac caccaaaaca acgtttggtt tttaatatga aatattatga agatttgact
      901 tacgaacaaa tgtcggcaat attgggtact tcggttggtg ccctcaaggc gagttatcat
      961 ctggccatta agaaattaga acattatctg gatatatatt aaaccttttt tctgtattat
     1021 tgtcaaacaa actgagaaga gggtacaaga aatgaaaacc aagaaggaaa tcgataggga
     1081 aatttctgaa aaacggcttg agtctcgttt tgggaaaaaa aattcttttc ctgttcctga
     1141 aggttacttt gccgaccttc cccaaaacat catacagaaa attaaatcgg aacaaacttt
     1201 taccctttct ttcgatcatt cccctcgtac tcaaagaaga tggttgatcc cagccattac
     1261 tatagctgtg gctgctttta tcggagcttt cttcttattc aagcccctta ctactcaggt
     1321 aaactcaatc gacaaacaag tggtttcgaa gggagatatt caaaacgatg cttctgacta
     1381 ctattacatt tctcacattt tgtataattt accttatgac catttattga atgctataaa
     1441 tgaagaagat gatacttacc tcgacagcgt catctattcg agaagcttga tccccgacgg
     1501 aacgctcaaa gaagaggagg ttatagatta tttgtattat cacatgtcga tttatgatat
     1561 aacacaattt taaaactatg aaagctcaaa gaatgatgaa aatactggga tgtggattgc
     1621 tgaacttagc attctttgca gtacctatta ttaccttagc tcagcctcct caagataacc
     1681 ccgacaaatg ggaacaaata aaagcccaaa aaattgctta cctcagtgca aagctcgagc
     1741 tgaagccttc cgaaagccag aaattctggc ccgtttataa tgaatactcc gaaaaacgtg
     1801 ataaattgct caatgaaatt tatcctaaac tttcgaaaca ggaattagac agactcatga
     1861 tgctttccga taaggaagct gctcaaagaa tggatgaata cttcattaaa caaacgcaat
     1921 tgcttgatct ggaaaagaaa tacaatgctt gttttcgcaa gattttaccc gatagtaaag
     1981 tgtttaggct ttatatggcc gaaatgatgt tcaaaaaaga attattggac gatatgcgcc
     2041 gctcgaaaca cgggccgaaa gaagaatatt agcagtgaat cgggatatct tgctgatcag
     2101 atttttttat tttgtgccat ttgaaatcgt ttcatagctt atccgctaat aaataatcta
     2161 atcgggtggt attaaaccct gtgaaggtgg attcttaggt gaaggtcggt tttgttcttc
     2221 agggtttctg gcatctatat ctttcagtaa aataagcttc ccatcggaaa aaagataggc
     2281 atccagaata ttcgccgcgg ctacattggc tcctactgtc ggttcatata ccagacgatt
     2341 catcacaatc atggcttctt ctttgatgac aggtttgcgt ttataccata ccttcttccg
     2401 ataatgttgt ttttcatatc ttaggacaaa ataactcccc gaagcatatt cgaatattag
     2461 acgagttttg ggtgattgat cgaaaatttt atctccgaat cgaggcaagt gatcatcatc
     2521 aaaacttagc acctcgatga tttttctttg tatgcggccg ttgcctccat cccagcccag
     2581 caggacatag aatttacctt tctccgattt gaattccaat agttgatagt acaaagcgcc
     2641 ataccagttt tcaggcctgg ttatgagagt ttcgggattt ttgatctcat ccgatttgtc
     2701 tttcagtgga taaaccgata attttgaatc gggtgtattc ttagctacta gtagcccatc
     2761 ataaaaataa cctcctgtcg aaagaggaat attccatgtg atcaatagaa aagctgagtc
     2821 tgtgggcatc aatgccgaga gtcttatttc tttgggcagt ttgagagaaa ggcactgagt
     2881 tgtggtaaaa ctttgagaaa gaatctcctt tactttgagc gatgcaaccc tttgcacact
     2941 gtcgctctcg ccccttagca ttatctgcca atatgcaagc aaagtatcct gattcgggtg
     3001 cactttttga gccttaatgc tttgatttga atataaattc accaaaacag gcaggaagag
     3061 atataatagg gttcttttca tatcatcttg atgtggaggc gagtgacaat ttaaaatatt
     3121 acttcataat ctcttagctt tgcaaaagta aagatttaaa aatgacaaag gcagaacgtt
     3181 tatttatatt gaaacgtttg ggaaatctgt tgaggtggat taccctgaca ggtgtttatt
     3241 ttactccgtc tgatgaagaa atcgagttat caaagaaatt gcaaagaata atcgatgaaa
     3301 atcatcattt taatctctgg tttgatcctt ttcatgtaaa atattcactt tctgcgcttg
     3361 gattgatgct cgacgaaacg agactcgaaa attgggctaa ccgctatatt attcccgaag
     3421 atagaatcaa caggaaaact attgccgtag tgtgcgctgg gaatatacca gcagtcgggt
     3481 ttcacgattt tttatgtgta ctcgtatcgg gtcataacat tctggtgaaa ttatcctccg
     3541 acgacctcct tttgttgcct tggttttctg aatttctgca taacaccaat cctgaattca
     3601 agaatatcat caccatcacg gagggtacaa tttcgggctt cgatgcggta atagccacag
     3661 gttcgaacaa tacttccagg tatttcgaat attattttgg gaaatatccc cacatatttc
     3721 gaaggaatat gaacagcatt gccattcttg atggacaaga atcgccacag gaactttcgg
     3781 gtcttgtcga tgatatttta atctattatg gccgtgggtg ccgtaatgtt tccttcctgc
     3841 tcgttcctga aaattatgat ttctcacctt tgctcgatgc ctttcggaaa tattctcatt
     3901 atgcctatca ttcgaagttt tataataatt acgaatacaa taaggctgtg atgttggtga
     3961 atcgcatccc cttctatgat ataggtttca tgctgctaac ttcccacccc tccattcaaa
     4021 gtccattggc agtgattcat tatcaaacat ataaaaaatc ggatgagata tactctttcc
     4081 tccattttaa cagggataag attcaacgta atgtcagtca tattcaggga attcccatta
     4141 aagtggattt tggaaaatca caacagcctg ctctggatga ctatgctgat ggggttgata
     4201 cgatgaagtt tctggagaat ttataaaata ttctgctctt catatttttt gttattacaa
     4261 aattttgtac ctttgccgcc cgaaaagttc aataaaaata tgaaaaaaga tattcatcct
     4321 tccgattatc gtttggttgt gttcaaggac atgtcgaacg attatgcatt tttatgccgt
     4381 tctactgcta aatcaaagga aaccattatt tgggaagatg gtaaagagta ccctttagtt
     4441 aaggttgaaa tttcacatac ttcgcatccc ttttttacgg ggaaacttac tcttgttgat
     4501 actgcgggaa gagtggataa gttcaagagc aggtatcaga aacattatca atccaaacaa
     4561 aaatagatta ttgcataaga aaatttagtt caaccagctt gtgtcaggct ggttttttta
     4621 atatattgac ctgcgccata ggtcaagggt tcgaaaccag ggatgttttg tgaagaaatt
     4681 tattttttcc agagaggatt ctgaccataa aaccaattaa aattcaatga tttatgacta
     4741 tctttgaaat agtaatgatg cattggttaa ttatttgtga ataaaaaatt actttattca
     4801 acctttgaat tataattttg tctgataatt gtagttagaa atagatgaca ggtgttcttg
     4861 ttttataata cactcaaatc gttagtataa agttatgaat tacattcttt ttgatgatta
     4921 ttcgcgcaac aatcttttgc ctttgacttt tttgaggcct gtggctgatt tactgacagg
     4981 gatacttacc atacgtcaga aatgggaata ttatttagga gacagcactt cgagtcttac
     5041 cgaggattat ttaatcgaaa aatacccttt atacaagggg aagaataata ttctcatcaa
     5101 tggatccgtt ttgcctagcg atgagctaat aaaagccatt cgaggtttac agcccaatca
     5161 ggctttgata aataaggatg ccatcattgc tctgcatgtt cctttggaaa atctggataa
     5221 gattggtgaa ggagacaccg agggtatcga agagatcgat acagatatag atatcatcaa
     5281 aatcaattat ttatgggatt tattcttgaa caatgcgaaa caaatcgaag ccgattatgc
     5341 tatgctgacc gtaggaagta aatctatcga gccaagtaag agtaatcata taattgctcc
     5401 tgagaatatt tttattgaag agggggcatt ggtggaaaat tgcaatttga atgctacaga
     5461 tggtcccata tatattgggc acaatgcaca aatttgggac ggagcaagcc tgagaggccc
     5521 tattgctgtg ggtgagcatg ccatcgtaaa aatgaatgca atcatttatc aagccactac
     5581 tatcggttct tattcaaaag ttggaggcga agtggagaat tctgtcatca tggcctactc
     5641 caataagcct cattcaggat atttgggcca ttcgatagta ggtcaatggt gtaatattgc
     5701 agcaggaacc aatgtggcta atctcaataa tacctataaa actattaaga tgtggaatta
     5761 tcctcaatct cgctttatag atactggatt acagttttgc gggcttgtat tgggcgatca
     5821 ttccaaaaca ggaatcaaca ctacctttaa tacgggttcg gtagtgggca ttagctcgaa
     5881 tgtgtttggt gccggatatc aacgcaattt tgtaccatct ttcgtatggg gaggccctag
     5941 tacaggttat tcaggctacg atttcgaaaa ggccatagaa accgcaaagg aagtctataa
     6001 acgacgcggt attgaactca ccgatgtcga cttcaaaatc ctccaacatg tatacgaaat
     6061 cacaaagaat aatataaggc tttaatttca cacccaaata aaaatgaaat cggtatctct
     6121 gaaagtcttc tgccgagata ccgattcttt tgtaaagcga
//
LOCUS       DAOORU010000143         6132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_15610_length_6132_cov_4.102024, whole genome shotgun sequence.
ACCESSION   DAOORU010000143 DAOORU010000000
VERSION     DAOORU010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15610_length_6132_cov_4.102024"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="PLF20_08480"
     CDS             complement(<1..794)
                     /locus_tag="PLF20_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOX81075.1"
                     /translation="MKKTKNFLIFFVIFSAFACTKTPTSKEKLIARRVDSVIKLMTVE
                     EKIGQLTLFTSDYDITGSTIRENYKEDIRQGRVGAIFNAYGVDYVRELQRMAVEETRL
                     GIPLIFGYDVIHGHRTIFPISLGEAASWDTAAVRRAARIAAIEATAEGLNWTFAPMVD
                     IARDPRWGRISEGAGEDTYLGMAMAKARVKGFQDNDLSSPYTMMACAKHYAAYGAAQA
                     GRDYNTVDVSDRTLREVYLPPFKACVEGRQIDFAQGTVEDIDRIIIP"
     gene            complement(802..1506)
                     /locus_tag="PLF20_08485"
     CDS             complement(802..1506)
                     /locus_tag="PLF20_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOX81076.1"
                     /translation="MILIPFVILSNGQDLKPGTQIPRAGKGIFVKKAQAYPYLLFLPN
                     NFVADHQHSYPLLLFLHGSGERGNDLELVKKNGTPGFVESMEDFPFVMLTPQCPQGET
                     WDSERLMALVNEAIENYSIDTNRMYITGLSMGGYGTFDLACNYPGKFAAILPVCCGGQ
                     KLKAEKLIHTPIWAFHGADDTIVPVSESKNMVEIVNRLGGNAHITILPGVGHNAWEYV
                     YIRPEIYQWLLSHQLK"
     gene            complement(2123..3283)
                     /locus_tag="PLF20_08490"
     CDS             complement(2123..3283)
                     /locus_tag="PLF20_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX81077.1"
                     /translation="MKKTLLSLFTMVALSMSMLGQTVTVTFNVNMTNAGLSDQNVYIA
                     GDFGATTWGSWNEPGTNPACLMTDEDGDMIYSITMDLPKGTYPFKFFKGEGWSGDEWP
                     GDPNRTATVKKAMNYSYVWAVRLDQPQILADFEDGTWGILTPHVMGCGDYDNPDLHPL
                     EETFMIVDNPSPDAMNHSAKVLKFIRRGTTNGGLPWGGYWANNNPSVDAQTNKYVHVK
                     LLKPRISPVKFKLEGGTSGTLEIFSENTQQLTNQWEEFVFKFDTMTGQYPISVLMPDF
                     EDPLTITDDIIIYIDDIRIDTIATVSNGIPSISNDKILIYPIPLTKDLTIENFGKIVR
                     VNVYNLAGQLIKQIETQPSSKVVINLEDLCSGMYLIYITNTEGKTITRKISK"
     gene            complement(3387..5783)
                     /locus_tag="PLF20_08495"
     CDS             complement(3387..5783)
                     /locus_tag="PLF20_08495"
                     /inference="COORDINATES: protein motif:HMM:NF021596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOX81078.1"
                     /translation="MKTKIFVNVHFFFILLIFQLNAQTWLYFQDSPSNIYYDYSWMEL
                     TSPSQLERCGNDLRKFPVESNTPPIQGVNSLRLKWTSNPGGNWYAIAAGTNWTDKDIS
                     QSDSLIFWLYSPTSQERNSLPKIFFEDIYNQKSIMIPMRNYVSQIPTAIWTRISIPMS
                     PFINQINPIDYTVIKTIGFAQNESDGIQHVLFIDDIRVIKGDGTSPSVSAPKGLFAKG
                     YERHVILKWNKNSECNVSDYEVYRSTDNGSSFQLAGTTKKTDTIFNDYITSKLSVTKL
                     TYGVKALNDMGQPSAMSELVETYLKNLSDEELLDMVQEATFRYFWEYGHPVSGMARER
                     LGSGEIVTTGGTGFGIMGILCGIHRNFITRQQGLERILKIVDFLSQTDRFHGAWPHWL
                     NGTSGKVIPFSNYDNGGDLVETAFLIQGLLTARSFFDGEYLEDTLRQKITQLWESVEW
                     DWYTRNGSNVLYWHWSPNYQWIMNMPIRGFNETMIVYILAIASPTHPVAASLYHMGWA
                     SGNYLNGKSFYGYPLAVGWDYGGPLFFTHYSFLGFDPRSKKDAYANYFNNNRNIALIH
                     HAYCIDNPLNHLGYGDSCWGLTASDDPDGYLAHQPHTNNDNGTISPTAALSSFPYTPV
                     ESMKALKYFYRKLPKTWGYYGFYDAFNQKRNWWATSYLAIDEGPILVMIENYRSALLW
                     NLFMKNPEINPALENIGFVYDPFGIDKIPQRNKYFELTPNPYQETISIRYLSNQPTIA
                     SIKIYNSAGQSIPLSEKTLYFNDDSKVKTLNLNSLKKGLYIITIEGNHSKETLKFTKK
                     "
     gene            complement(5845..>6132)
                     /locus_tag="PLF20_08500"
     CDS             complement(5845..>6132)
                     /locus_tag="PLF20_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOX81079.1"
                     /translation="KIHQGDVSGAATYLNKVRQRVNLPVISNPTLDDILKERRLELAM
                     EGDRFFDLVRTGKAAEVLGPLGFTPNKNEVFPIPSQQIELSQGKLTQNQGY"
BASE COUNT         1712 a         1188 c         1318 g         1914 t
ORIGIN      
        1 gggattataa ttctgtcgat gtcttcgacc gtaccctgcg cgaagtctat ctgcctccct
       61 tcaacgcagg ctttgaaggg aggcagatag acttcgcgca gggtacggtc ggagacatcg
      121 acagtattat aatcccggcc ggcctgagct gccccataag cggcataatg ttttgcacag
      181 gccatcattg tataagggct ggacaagtcg ttatcctgga atcccttcac tctggctttg
      241 gccatagcca ttccgaggta agtatcttca ccggcacctt ctgatattct tccccaacgt
      301 ggatcgcgag caatatccac cattggagca aaagtccagt tgaggccttc ggctgtagcc
      361 tcgatggcag caattcgtgc ggctcgacgc acggctgccg tatcccaact tgcagcttct
      421 cccagtgaaa taggaaatat agtgcgatgg ccgtgaatga catcatatcc gaaaatcaat
      481 gggatcccca atcgagtttc ttcgaccgcc atccgctgta actcccttac ataatctaca
      541 ccataagcgt tgaatattgc tcccacacga ccttggcgga tatcttcctt gtaattttcg
      601 cgtattgtgg atcctgtaat gtcgtaatcg cttgtaaaga gagtaagctg gccgattttt
      661 tcttcaacag tcatcaattt tattaccgaa tcgactcgcc tggcaatgag tttttcttta
      721 ctggtaggcg tttttgtgca tgcaaaagca ctgaatatca cgaaaaatat aagaaaattt
      781 ttagttttct tcattttaat cttattttaa ttgatgtgat aataaccatt gatatatttc
      841 tggacgaatg taaacgtatt cccaagcatt gtggccaaca ccaggtagaa tggtaatatg
      901 tgcatttcca ccaagtctgt tcactatttc caccatgttt ttcgattcac tcaccggaac
      961 gatagtatcg tcagcaccgt gaaatgccca gatgggtgtg tgtatgagtt tttcggcttt
     1021 taatttttga cctccacagc acacgggcag tatagcagca aattttccgg gataattgca
     1081 ggcaaggtcg aaagttccat aaccgcccat acttaaacca gttatataca ttctattggt
     1141 atcgatagaa tagttttcga tcgcttcgtt gactaatgcc atcaggcgtt cactgtccca
     1201 tgtttctcct tgggggcatt gtggtgttag cattacaaaa ggaaaatctt ccatggattc
     1261 gacgaatcct ggcgtgccat tttttttgac caactcgaga tcattgcctc tttcgcccga
     1321 accgtgaaga aaaagtaaga gaggataaga atgttgatga tcggcgacga agttattggg
     1381 taaaaataga agatatgggt aggcctgagc ctttttgacg aaaatgcctt tacccgctcg
     1441 aggaatttga gttccgggtt tcaagtcttg gccattcgat agaatgacaa aagggattag
     1501 aatcataaga agaaaggtga tttttaccat atcatgcaaa tattaactgt aaaatctatt
     1561 tcgtcggcat taagatacga ccgggcagtt ttgcttatcc aatgaattga atgtttagct
     1621 atccataccg tctgaaattt cggagaattt tcagttaagt ttcagaatag ttcgatcgaa
     1681 aatctgctcc ttggattcgc tgcattcgcg gcatggtgta tatggcatgt gataaaaagt
     1741 tgttgataga gggttttatg taaatttagt tctcgaagtc aaatatccat ttcgtcccat
     1801 tatgaagctt ctccagttat ttttttataa actttatgaa ctttttgctt cttcaaaaat
     1861 gggggtagca tgatgaacga ttgtaaatga tgtactaaaa taaccatggg ttccagcgtt
     1921 tgcttcctat gcatttcata atttttgaga tatgttggct tggaatttca ttcaaaagaa
     1981 tgaattcaaa gaaaatcccc ttattctgca tttttatcat gatatctctt ctcattaaaa
     2041 ggatttaaga gttaagaaac ttgattaatg caaattgagg ggataatcag atgaaagcaa
     2101 atgcttttac aattgttgct aattatttgc taatctttct tgtaatggtt ttcccttcgg
     2161 tgttggtgat ataaatcaga tacattccgc tgcatagatc ttcgagattg atgaccactt
     2221 tggaagatgg ctgggtttcg atttgtttaa tcagttgtcc ggcaagatta taaacattga
     2281 ctcgaacaat ctttccaaag ttctctatag taagatcttt tgttagggga ataggataga
     2341 ttaagatttt gtcattcgat atagaaggta ttccattgga tacagtggca atcgtatcga
     2401 tacgaatatc gtcgatgtag atgatgatat cgtcagtaat agtgaggggg tcttcgaaat
     2461 cgggcataag aacggagatg ggatattgac cggtcatggt atcaaattta aatacgaatt
     2521 cttcccattg gttggtaagt tgttgagtat tttcactaaa gatttcgagt gtacctgatg
     2581 taccaccttc gagtttgaat ttaacaggag agatacgtgg tttcaagagt tttacatgca
     2641 catatttgtt agtctgagca tctacactgg gattattatt tgcccagtat cccccccaag
     2701 gcaatcctcc attggttgtt cctctacgga tgaatttcag cacttttgcg ctatggttca
     2761 ttgcgtcggg agagggatta tcaacaatca tgaatgtttc ttctaaggga tggagatcgg
     2821 ggttgtcgta atcgccgcaa cccataacgt gaggggtcaa aattccccat gttccatctt
     2881 caaagtcggc caaaatttgg ggttggtcta acctaacagc ccaaacatag gagtaattca
     2941 tcgctttttt aacagtagct gtacgattgg ggtcgcctgg ccattcgtca ccactccaac
     3001 cttcaccttt gaaaaattta aaaggatagg tacctttagg gaggtccata gtaatagagt
     3061 aaatcatatc accatcttca tccgtcatta agcaagctgg attggtccct ggttcattcc
     3121 aactacccca tgttgttgct ccaaaatcgc cagcaatgta aacattttgg tcgctcaaac
     3181 cagcattggt catatttaca ttaaaggtta ctgttacggt ttgtcccagc atactcatgc
     3241 tgagagctac catcgtaaat aatgaaagta aagttttctt cataatttta gtttttaggt
     3301 ttaacagaat aattcgtagc aaatttaaac aatatttatc cgaaaattcc atcttacttt
     3361 aaattttttt aaaaaaagta atttttttat tttttggtaa atttaagagt ctcctttgaa
     3421 tgattgcctt cgatggtaat gatatacaat cctttcttta atgagtttaa attaagggtt
     3481 ttaaccttag aatcatcgtt gaaataaagt gttttttctg ataatggaat cgactgtccc
     3541 gcagagttat aaattttgat cgaagcaatc gtaggttggt tcgaaagata tctaatggaa
     3601 atagtttctt gatatggatt gggagttagt tcgaagtatt tgtttctttg aggaatttta
     3661 tcaatgccaa aggggtcgta aacgaagcca atattttcaa gggcaggatt tatctcggga
     3721 tttttcataa atagattcca aagtaaggct gaacgataat tttcgatcat gaccaatatt
     3781 gggccttcat caatagccag gtaggaggta gcccaccagt tacgtttttg attgaaggcg
     3841 tcatagaatc cataatatcc ccatgtttta ggaagcttac ggtagaaata ttttaaggct
     3901 ttcatggatt caaccggggt ataagggaat gagctcaagg cggcggttgg agaaatagtg
     3961 ccattgtcgt tattagtatg aggctgatgg gctaaatatc cgtcaggatc atcgctagct
     4021 gtcaatcccc agcatgaatc tccataaccc aaatgattca gggggttatc gatacaataa
     4081 gcatggtgga ttaaggctat attgcgatta ttattgaaat aattggcata ggcatctttt
     4141 ttacttcttg ggtcgaaacc taaaaaggaa taatgagtga agaaaagcgg tcctccatag
     4201 tcccaaccta ctgcgagggg atatccatag aatgattttc cattgaggta atttccactt
     4261 gcccatccca tgtgatataa tgaagctgcc actggatgag taggggaggc gatggccaga
     4321 atataaacaa tcatagtttc gttgaagccc cgtatgggca tattcattat ccattggtaa
     4381 tttggcgacc agtgccagta aagtacatta cttccatttc gggtgtacca atcccattcg
     4441 acgctttccc ataattgagt aattttttga cgtaaggtat cttcgagata ttctccatca
     4501 aagaagctac gagcggttaa aagtccttga atcaagaagg cagtttccac cagatcacct
     4561 ccattatcgt aatttgaaaa cgggataacc ttcccgctgg ttccattcag ccagtgaggc
     4621 catgcaccat gaaatctgtc ggtttgggag agaaaatcaa cgattttcaa aattctttcc
     4681 aatccttgct gtctggttat aaaatttcta tgaataccgc ataatatgcc cataattcca
     4741 aaacctgttc caccagtagt aacgatttca cccgatccaa gcctttcgcg tgccatacct
     4801 gacaccgggt gtccatattc ccagaaatat ctgaaggtgg cttcctgaac catatcgaga
     4861 agttcctcat cgctcaggtt tttgagataa gtttcgacca gctccgacat tgccgaaggt
     4921 tgtcccatat cattcaaggc ttttacacca taagttaatt tggtaaccga aagtttggaa
     4981 gtaatataat cattaaatat tgtgtcggtt ttcttggtgg ttcctgccaa ttgaaaactt
     5041 gagccgttgt cggtcgaacg ataaacctca tagtcactga cattgcattc agaattcttg
     5101 ttccatttga gtatcacatg tcgttcgtat cctttggcga acaggccttt cggagcgctc
     5161 accgatgggc tggttccatc tccctttata acacggatat cgtcgatgaa aagcacgtgt
     5221 tgtattccat cgctttcgtt ctgagcaaaa cctattgttt ttatgacggt gtaatcgata
     5281 ggattgattt ggttgatgaa aggagacatg ggtatgctta ttcttgtcca tatagctgtc
     5341 ggaatttgag aaacataatt tctcatggga atcataatgg atttttgatt gtagatatct
     5401 tcgaagaaaa tttttggaag tgaattgcgt tcctggctag taggggaata gagccagaag
     5461 ataagggaat cggactgaga gatatctttg tcggtccagt tggtgccagc ggcaatggca
     5521 taccagttac ctccagggtt ggatgtccat tttaatctca gactattaac accctgaatg
     5581 gggggggtgt tactttcgac gggaaatttt cgcaaatcgt tgccacagcg ttcgagttgg
     5641 ctgggagagg tgagttccat ccaactgtaa tcgtaataga tattagaggg gctatcttga
     5701 aaatagagcc aggtttgtgc attaagttgg aaaatcagaa gaatgaaaaa gaaatgaacg
     5761 ttgacaaaga ttttggtttt catcgggttg tagttatgga gataaagttg cgggctggct
     5821 gcaggccggc ccgcatagta aaaatcaata cccctgattt tgggtcagct tgccttgtga
     5881 aagctcgatt tgttgactcg gaatcggaaa tacctcattt ttgttgggcg taaatccaag
     5941 aggcccaagt acctcagcgg ctttccctgt tctaaccaaa tcaaagaaac ggtcaccttc
     6001 catggcaagt tctaaacggc gttcctttaa tatatcatcg agagtaggat tgctgatgac
     6061 cggaagattt accctttgac gcactttatt caggtaggta gcagcacccg aaacatcacc
     6121 ctgatggatt tt
//
LOCUS       DAOORU010000144         6119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_15642_length_6119_cov_5.985653, whole genome shotgun sequence.
ACCESSION   DAOORU010000144 DAOORU010000000
VERSION     DAOORU010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15642_length_6119_cov_5.985653"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="PLF20_08505"
     CDS             <1..730
                     /locus_tag="PLF20_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOX81080.1"
                     /translation="VHDDVVDDSMERRGFFSINALWKNKIAVLVGDYLLAKGLLLAME
                     NEDYALLKIVSEATLEMSEGELMQMEKARRLDIDEQVYLEIIRKKTASLIASCCACGA
                     ASSGASQELVFRMKTFGEKTGIAFQIQDDLFDYQPTNCTGKPRGIDFRDQKLTLPLIY
                     TLDHCSYSEKRTIIRTLKKDGENRHKVGEIINLVEKYGGLDYAHDKMLNYRNQALELL
                     DVLPASPALDSLRELVVYITHRER"
     gene            1035..3323
                     /locus_tag="PLF20_08510"
     CDS             1035..3323
                     /locus_tag="PLF20_08510"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008627187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOX81081.1"
                     /translation="MSSISDKNLLGRLALEYHSEGRPGKIEVKPTKPYHSQQDLSLAY
                     TPGVATPCLEIKKNLDDVYKYTAKGNLVAVISNGTAVLGLGDIGPVAGKPVMEGKGLL
                     FKVFADIDVFDIEIDERDPKKLIQIIKAISLTFGGINLEDIKAPECFEIEERLMEELD
                     IPLMHDDQHGTAIITSAGLLNAIELAGKDIGKMRIVINGAGAAAISCTKLYLKLGARL
                     ENITMFDSKGMLHVGRTDLNPYKKQFARDGKILGLEEALDGADMFLGLSVAGVLKKEW
                     LKKMAPNPIVFALANPVPEITYEEATSTRDDIIMATGRSDYPNQINNVLGFPFIFRGA
                     LDVRSTKINDEMKLAAARALAALAKEPTPDEVNSAYGVTNLKFGKDYIIPKPNDPRLI
                     LHVAPAVAKAAMESGVARNPILDWDQYQEELNNRMGQGNPVIRQIRTMAQRHPKRIVF
                     AEAENYKILKAAEICLIEGYAKPILLGNKSYIEKLVQQNELELNGVEIIDPRAEEEQE
                     RREEFANLFYEKRHRKGVTYLMACERMHHRNYFGPMLVETGFADGMISGLTTSYPDTI
                     RPALQIIGKQDRWNVVSGMYILHTIKGTLFFADTTVNLNPSTETLVEITLQVAEAVRN
                     FKIEPRVALVSYSNFGSVKGNTPDKVQQAVSMLHNNYSDLIVDGDIQADFALNTEMMK
                     EYFPWCKLADAPANVFIFPYLTAGNAAYKLVQQLTDQNAIGPVLLGLKKPVHVLQMGS
                     TVNNIVNMVSLATIDAQRLSEK"
     gene            3967..5604
                     /gene="pckA"
                     /locus_tag="PLF20_08515"
     CDS             3967..5604
                     /gene="pckA"
                     /locus_tag="PLF20_08515"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOX81082.1"
                     /translation="MQKRSEKIRSELESYGLEGIQEIYYNLSYDELFRHETDPSLEGY
                     ERGFVTNTGAVAVDTGIFTGRSPKDKYIVDEDTSREHIWWKSPTHKSSDNKPISEAVW
                     RDLYAISAKQLSHKKIYVMDGFAGANPDTRINIRVVTEVAWMAHFVKNMFIRPTEEEL
                     ENFEPDFIMLNACKTTDPYWKEHGLNSEVFVAFNLKARRAVVGGTWYGGEIKKGFFSV
                     MNYYLPLKGIASMHCSANVGKEGDVAIFFGLSGTGKTTLSTDPKRLLIGDDEHGWDDD
                     GIFNFEGGCYAKCINLSKENEPDIYHAIRRDALLENVVYDPKTGEIDFSSAAKTENTR
                     VSYPIYHIKNIVKPVSKAGHAKKIIFLTADAFGVLPPVAKLTEDQTLYYFLTGYTAKV
                     AGTERGIKEPSPTFSSCFGAAFLLLHPTVYARELSRKIKEYKSEAYLVNTGWIGGPYG
                     QGHRIDIPSTRAIIDAILDGSINEAEFETLPYFHLLVPKSLKGVESKILDQRSTWPNP
                     EAWDKAARQLAQKFIDNFKNFTDEEEGQRLVSAGPQL"
     gene            5683..>6119
                     /locus_tag="PLF20_08520"
     CDS             5683..>6119
                     /locus_tag="PLF20_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOX81083.1"
                     /translation="MNKITIIGAGNVGATVANVIAHKDLCRELILLDIKEGLAEGKAL
                     DIWQTGPINHFNTKVIGVTNDYLKTKGSGVVVVTSGIPRKPGMSRDDLISTNALIVKE
                     VVEKSIKYSPETILIIVSNPLDIMTYVAYKAASKSRNKVFGMAG"
BASE COUNT         1908 a         1185 c         1318 g         1708 t
ORIGIN      
        1 ggttcacgac gatgtggtgg atgactcgat ggagcgaagg ggtttttttt caatcaatgc
       61 cttatggaaa aacaaaatcg cggtgcttgt tggtgattac ttactggcta agggccttct
      121 attggccatg gaaaatgaag attacgccct gttgaaaatt gtttcggaag ccacactcga
      181 aatgagcgaa ggagaactca tgcaaatgga aaaagctcgc cgtttagata tagatgaaca
      241 agtctatttg gaaattatac gaaaaaagac tgccagcctt attgcctcat gctgtgcttg
      301 tggagctgca tcctcgggtg ccagtcaaga gctcgtgttc aggatgaaaa cctttggtga
      361 aaagacgggt attgcctttc agatacagga tgatcttttt gattatcagc caaccaattg
      421 tacgggaaaa cccaggggaa tcgactttcg agatcaaaaa ctcacacttc ctctcatcta
      481 tactttggac cattgctctt attctgagaa aaggacaatc attcgtacct tgaagaaaga
      541 tggtgaaaat cggcacaaag ttggagaaat catcaattta gtagagaaat atggaggact
      601 ggattatgcc catgataaaa tgttgaatta tcgcaaccag gcactcgaac tgctcgatgt
      661 tttgcctgcc tctcctgctc tcgattcctt gagggaattg gtcgtttata tcactcatag
      721 agaaagatga tatttcgtta tattctcttc gaacttactt atacatcgaa cattttagat
      781 ttggcctctc aaaagacgga gaatacctcg aagttcatta aaaatactga aaaaataact
      841 tgagtagtag taaaatattc atcctcaaag aatactcata gaaaatctca tttcaatggg
      901 gaaatatttt tctagcaaat aaaatttttt agaaaatcca atttttattt agaaaagtat
      961 tcattgcaac gaaaagatag agtagctttg ctttagatat tttcgaatca tttcaccaaa
     1021 agctatttta tattatgagt tccataagcg acaagaattt attaggcaga ctggctttgg
     1081 agtatcattc tgaaggaagg ccagggaaaa tagaggtaaa acctaccaaa ccttatcata
     1141 gtcagcagga tttatcgctg gcttacacac ctggagtagc cactccttgc ctcgaaatca
     1201 agaagaacct tgatgatgta tacaaataca ccgcaaaggg caatttggta gcagtaatca
     1261 gtaatggcac ggcggttctt ggacttggcg atattgggcc cgtagcaggc aagccagtta
     1321 tggaggggaa gggtttatta ttcaaagtat ttgccgatat agatgtattt gatatcgaga
     1381 ttgatgaaag agatcccaag aaactcattc agattatcaa ggcaatttct ctaacttttg
     1441 gaggaatcaa tctggaagat ataaaagctc ccgaatgctt cgagattgaa gaaaggctaa
     1501 tggaagagct cgacattcct ttgatgcacg acgatcagca tggaaccgcc atcattacct
     1561 cggctggcct gctcaatgca atcgagttag caggcaaaga catcggtaaa atgcgaatcg
     1621 tgatcaacgg agcaggagcc gcggccattt catgcaccaa actctattta aaattaggtg
     1681 ctcgtctcga aaacatcacc atgttcgata gcaaaggcat gctccatgtc ggccgtaccg
     1741 atttgaatcc ctataaaaaa caatttgcca gagatggaaa gatattagga ctcgaggaag
     1801 ccttagacgg tgccgatatg tttcttggtc tttcggtagc gggagttttg aagaaagaat
     1861 ggctgaaaaa gatggctcct aaccccatcg tttttgcctt agccaatcca gtacccgaaa
     1921 ttacttatga ggaagccact tcaactcgtg atgatatcat tatggcaacc gggcgcagcg
     1981 attatcccaa tcaaatcaac aatgtgctgg gtttcccatt tattttcaga ggtgctcttg
     2041 atgtacgatc cactaaaatt aacgacgaga tgaagctggc ggctgcccgg gcgcttgcag
     2101 cattagccaa ggaacctaca cccgacgaag taaactcggc ttatggagtg accaacttaa
     2161 aattcgggaa agattatatt attcccaaac ccaatgaccc acgtcttatt ctacatgtag
     2221 ctccggcagt agctaaagct gccatggaat ccggtgttgc ccgcaaccct atcctcgatt
     2281 gggatcagta tcaggaagaa ttgaataacc gcatgggtca gggaaatcct gttatcaggc
     2341 agattagaac aatggcgcag agacatccca aacgaatcgt atttgccgaa gccgaaaatt
     2401 acaaaatact caaggctgct gagatttgcc tcatagaagg ttatgccaaa cccattttac
     2461 ttggcaataa atcttatatc gaaaaactcg ttcagcagaa tgaactcgaa ttaaatggag
     2521 tggaaattat cgatccacgg gctgaagaag aacaagaacg tcgtgaagag tttgcaaatc
     2581 ttttttacga aaaaagacat cgcaagggag ttacttacct aatggcctgc gaacggatgc
     2641 accacagaaa ttattttggc cctatgctgg ttgaaaccgg cttcgctgat ggaatgatat
     2701 ctggccttac taccagttat cccgacacca ttagacctgc acttcaaatc atcggcaaac
     2761 aggatagatg gaacgtagtt tcaggaatgt atattcttca tacaattaaa ggtactttgt
     2821 tttttgccga tacaaccgtc aacttgaatc cttcgactga aaccctggtc gagatcaccc
     2881 tccaggtagc cgaagccgta agaaacttca aaatagaacc ccgtgtggct ctcgtttcct
     2941 attccaattt tggttcggtg aaagggaata caccagataa ggttcaacaa gcagtttcta
     3001 tgctccataa caattattct gatcttattg ttgatggaga cattcaggcc gattttgcac
     3061 tgaatactga gatgatgaag gaatatttcc catggtgcaa attggccgat gcccctgcca
     3121 atgtatttat ttttccctat cttacagctg gaaatgctgc ctataaactc gttcaacaac
     3181 tgaccgacca gaatgcaata ggacctgttc ttcttggcct taaaaaacct gtccatgtcc
     3241 ttcaaatggg aagtacggtc aacaacatcg tcaatatggt cagcttggct acaattgatg
     3301 cacagcgcct ctcggaaaaa tagaggtttt ttcgagccga ataatttatc agctctttcc
     3361 ctatgatgaa agaagaaaag aataagtaaa atccaatcat tgaaaagata atccttatgt
     3421 attgacttca ttctaaaggg gtcaacatgc tccgaaatat ccacaataat gatattgtaa
     3481 tgattagcca tccatagggt tatacgaaat gaatttcatg acaaaatata agcaaagctg
     3541 tcaatctatc ttgatgagtt tattttggta gaataggttt ctgatgtttg cactttcttc
     3601 aggtttttcg tccttagggt cgtagtagtg aaatattcca aatttaggct ggagtgggaa
     3661 atcttataca tgaaaccgga atatttcttc aaaaaatttt tgtgttttga taaatggatt
     3721 tcaggtcatg aaccaaaatt ttattcgtga gaaattttaa tttatttttc tttaaggata
     3781 atgaaaatca agttagtttc agtaaaacag gttaatagat cgatgagagt ttttcctggc
     3841 cataaaggat aaatcttcca tcctaactta aacccagtag aatagtagaa aagtttatag
     3901 ttttgccgag aattaatata gaactttata taaagatttt tacattttaa cttcaatata
     3961 taaattatgc aaaaaagaag cgaaaaaatc aggagcgagc ttgagtctta tggtcttgaa
     4021 ggcattcagg agatttacta caatttgagt tatgatgaat tgtttcggca tgaaacagat
     4081 ccttcgctcg aagggtatga acgtggtttc gtaaccaata cgggggcagt ggcagtagac
     4141 acaggaattt ttactggccg ttcgcccaaa gataaataca ttgtggatga agacacttca
     4201 cgtgagcata tctggtggaa atcgccgaca cataaatcct ccgacaataa acccatttcg
     4261 gaagcagttt ggagagatct ttatgcgatt tcagccaaac aacttagtca taaaaagata
     4321 tacgtcatgg acggatttgc gggtgcaaat ccagatacac gtatcaacat tcgtgtagtt
     4381 actgaagtag catggatggc tcattttgtt aaaaatatgt tcatcaggcc aaccgaagaa
     4441 gagttggaaa acttcgagcc cgattttatc atgctgaatg cttgcaaaac cactgatcct
     4501 tattggaagg agcatggttt gaacagtgag gtttttgttg cctttaattt gaaggcaaga
     4561 agagccgtgg tgggtggaac ttggtatgga ggagagataa agaaaggatt tttctcggtc
     4621 atgaattatt atttgccttt aaaaggcatt gcttctatgc attgttcggc caacgttggt
     4681 aaagagggcg atgttgccat attcttcggg ctgtcgggga ctgggaaaac cacactttcc
     4741 accgatccca aacggcttct cattggcgat gatgaacatg gctgggacga tgatggtatc
     4801 ttcaatttcg aaggcggatg ctatgccaaa tgcataaact taagcaagga aaacgagcct
     4861 gatatatatc atgcaatacg ccgggatgcc ttgctcgaaa atgtggtata cgatccaaaa
     4921 acaggggaga tcgacttttc gagtgcggca aaaacagaaa acacccgggt ttcctatcct
     4981 atatatcata tcaagaatat tgtaaaaccc gtttcgaagg cagggcatgc aaagaagatt
     5041 atttttttga cagccgatgc tttcggagta cttccacctg tggcgaagct gacagaagat
     5101 caaactcttt attattttct aactggatat acggccaaag tggcaggtac cgaaagaggg
     5161 ataaaggaac cctctcctac attctcttct tgttttggtg cggcttttct tctgttgcat
     5221 cctacggttt atgcacgtga actttcgcgt aaaataaaag agtacaaaag tgaagcctat
     5281 ttagtgaata caggctggat aggagggcct tacggtcagg gtcatcgcat cgacattcct
     5341 tctacacgtg ccattatcga cgcaattctc gacgggtcaa tcaacgaagc cgaattcgaa
     5401 actcttcctt attttcatct ccttgttcct aagagtctta aaggtgttga aagtaagatt
     5461 ttagatcaaa gatcaacttg gcccaaccct gaagcatggg acaaagccgc tcgtcaattg
     5521 gcgcagaagt ttatcgataa tttcaagaac ttcaccgacg aagaagaagg gcaaaggctt
     5581 gtttcggctg ggccacaatt ataagaagaa actttcaaca atttcttcga tctgctgtta
     5641 tattcctaaa cagttaattc tttccctcaa tatgcattta ctatgaacaa gattacaatc
     5701 attggcgctg ggaatgtggg agctactgta gccaacgtga tagcccataa agatctatgc
     5761 agggaactga ttttactgga tattaaggaa ggactggccg aagggaaagc tctggatatc
     5821 tggcaaaccg gccctatcaa ccattttaat accaaagtaa ttggtgtgac taatgattat
     5881 ctgaaaacta aaggttcagg cgttgtggtg gtaacttcag gtattccgcg taaacctgga
     5941 atgagccggg acgatttaat cagtaccaat gccttgatcg ttaaagaagt agtggaaaaa
     6001 tcaataaaat attcgcccga aactatactc atcatcgttt cgaatccatt ggacataatg
     6061 acttatgtag cctataaagc ggcttcaaaa tcgaggaaca aagtatttgg aatggcggg
//
LOCUS       DAOORU010000145         5981 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_16114_length_5981_cov_4.833615, whole genome shotgun sequence.
ACCESSION   DAOORU010000145 DAOORU010000000
VERSION     DAOORU010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16114_length_5981_cov_4.833615"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2302
                     /locus_tag="PLF20_08525"
     CDS             <1..2302
                     /locus_tag="PLF20_08525"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOX81084.1"
                     /translation="SHILADLSVHPTNCYDTCDGWAKVNPSGGFSPYSILFSNGIEDS
                     VAANLCSGQYWVSIIDSIGCDTLIEFRIDSPSPFFINYELQHVSCHGDSTGFIHLTVM
                     GATPPYEVLWSNGDSGLYLNNLPAGIYTAQIFDANQCDTLLNFSIQQPEAFGVEAEIN
                     QVSCFGYSDGSIHLQISGGISPYSVIWNNGMQGASINGLHAGTYTALLTDSLQCDTSM
                     TFILTEPPPFFIQAIVVPVSCPGYHDGSVELLLQGGSQPYFIHWVTGDTSHHLENLPV
                     GFYSVRIIDAHQCIFDTGFIIDEPVPFSIIAEITPVTCKGYADGSIMIEASGGNPPYT
                     LTWSNGATGNFLSNLTGGFYTLTLFDAHSCDTTITFLVPEAHQPLNVSYSSYQPSCWG
                     YQDGAYKILPEGGMPPYSIFWGMDEISDSIGNLPAGIYSFEISDAYKCRVNITDTLSS
                     PTPFMIQSIIHHPSCAFGVPDGSIQLLVSGGTPSYYFHWSTGEQTSLLQYIPAGTYEV
                     DLIDSHQCDTNLSFTLLAPSDFQIIKKISHCSCFGNNDGSIAIEIAGNQSDYQILWGD
                     GSNENIRHNFYAGIYPFRVSDKNDCTKYDTAVIEQPEAIEVIKRVTPVGCESDSDGKI
                     ELSIGGGSPPFTVIWLEGFQGEMLYELPKGIYTFQIIDNNNCLVKDTVIVWQKDCCID
                     IPNVITPNSDGFNDKFVIKGIENFPSNELIIYNRWGKEIIQFKGYKNEWDGRNANGEQ
                     VAEGVYYYVLHLTGGRYFQGSITVIR"
     gene            2518..3438
                     /locus_tag="PLF20_08530"
     CDS             2518..3438
                     /locus_tag="PLF20_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOX81085.1"
                     /translation="MKKILVATEKPFAKEAIAAIRKVVEDAGYTLALLEKYTSKTELC
                     NSIADADALIIRSDIVDKEVVEAAKNLKIVVRAGAGYDNIDLEACTKHGIVAMNTPGQ
                     NSNAVAELAFGMMLYMARGGFDGGTGTELRGKTLGIHGYGNVGRYIGKIALGFGMKTY
                     AFRRTSMDEIIKAEGIIPLSKAEDIYKNCQYVTLHIPANEQTKKSINYDLLSLMPKGA
                     TLVNTARKEIIDEESLLKMFAERPDFKYLSDIEPACKAEIIEKYSKRYFFTPKKMGAQ
                     TLEANNNAGIAAAQQIIDFFEKGDVRFKVN"
     gene            3717..4136
                     /locus_tag="PLF20_08535"
     CDS             3717..4136
                     /locus_tag="PLF20_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOX81086.1"
                     /translation="MTVFRFQDLRVYQKTLEFAEWASRIAEGHSSNGQGQIISNKLLD
                     SAVGIVFCIAEGSASPRPVFIEQLKKAKSHVRECIVMSQLMLSLKLLNERNYEYCHST
                     LSEIIRMIGGLIASLSKEQADAPRDDRGNEHKNKDTL"
     gene            4175..5422
                     /locus_tag="PLF20_08540"
     CDS             4175..5422
                     /locus_tag="PLF20_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOX81087.1"
                     /translation="MAILKPFKGLRPPKEIAHLVAARPYDVLNSNEAREEARGNPYSL
                     LHITKPEIDLPMGTDEHAPEVYQKASENFRMFRQKDWLVPDERDLLYIYAQTMDGRTQ
                     YGIVGCAAVDDYFNGVIKRHELTRKDKEEDRMKHVMITNANLEPVFFTYPAVNEIDKI
                     VDKIVKSTEPVYDFIAPDGIGHHFWLIESDETIQEIVRLFAKIPNTYVADGHHRTAAA
                     ALVGAEKRKMNPNHRGDEEYNFFMAVHFPDNQLRIIDYNRVVKDLNGLSKEEFIEKIK
                     QHFVIAEKGQQPYKPFHLHEFSMYLDGTWYSLEALGHTYNENDPIEVLDVTILSRYVL
                     DEILGIKDLRTDKRIDFVGGIRGMQELSRRVDSGEMAVAFALYPVTLKQLIAIADSGK
                     IMPPKTTWFEPKLRSGLVVHLLD"
     gene            5711..>5981
                     /locus_tag="PLF20_08545"
     CDS             5711..>5981
                     /locus_tag="PLF20_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOX81088.1"
                     /translation="MNRLSDKVAIITGAARGMGKEEAKLFAKEGAKVVVADIIENEAI
                     EVAKEIAENGGEAMACKLDVSNAKNWERMVEEISNKWGKIDILVNN"
BASE COUNT         1871 a         1240 c         1211 g         1659 t
ORIGIN      
        1 tagccatata ttggccgact tatccgtcca tccgacaaac tgttacgata cttgcgacgg
       61 atgggcaaaa gttaatcctt caggtggatt ttctccttat tcgattttat ttagcaatgg
      121 aatagaagat tccgttgctg caaatctttg ctcaggacaa tattgggtaa gcattataga
      181 ttctattggt tgtgacacct taatagaatt tcgaatcgat tccccttcgc catttttcat
      241 aaattacgaa ctacagcatg taagctgtca tggcgattcc actggtttta ttcatctaac
      301 cgtaatggga gcaactcctc cctatgaagt gttgtggtcg aatggagata gtggacttta
      361 cctcaataat ctacctgcag gcatatacac agcccaaata ttcgatgcca atcaatgtga
      421 tactctattg aacttttcca ttcaacagcc cgaagccttt ggcgttgaag ccgagatcaa
      481 tcaagtcagt tgttttggat attctgacgg tagcatccat ttacaaatca gtgggggaat
      541 ttcaccctat tcggttattt ggaataatgg catgcaagga gcatcgatca atggattaca
      601 tgcaggtaca tacacagctt tattgaccga cagccttcaa tgtgatacat ccatgacatt
      661 catccttacc gaaccgcctc ctttctttat tcaagccatt gtcgtgccgg taagctgccc
      721 gggataccac gacgggagcg tcgaacttct attgcaagga ggcagtcaac catatttcat
      781 acattgggtt acaggagata cttctcatca tctcgagaac cttccggtcg gattttattc
      841 cgttcggata atagatgctc atcaatgtat tttcgatacg ggtttcatca tcgatgagcc
      901 agtaccgttt tccataatag ccgaaattac acccgtaact tgcaaaggat atgctgatgg
      961 tagcataatg atcgaagcga gtggaggtaa cccgccatat actttgacat ggtcgaatgg
     1021 tgcaacggga aattttttgt caaatctaac tggtggcttt tacactttaa cccttttcga
     1081 cgctcattca tgtgatacca ctatcacatt ccttgttccc gaggctcacc aacctctaaa
     1141 tgtatcctat tcgagttatc agccttcctg ttggggttat caggatgggg cttataaaat
     1201 tttaccagaa ggcggaatgc ctccctacag cattttttgg ggaatggatg aaatatcaga
     1261 cagcataggc aacttacccg ctggaattta ttcattcgaa atttccgatg cctataaatg
     1321 cagggtcaat atcacggata ctctttcctc ccctactccc ttcatgattc aatccattat
     1381 tcatcatccc agctgtgcat ttggggttcc tgacggaagc attcaacttc tcgtctcagg
     1441 aggcacaccc tcttattatt ttcattggtc aaccggtgaa caaacttcct tactgcaata
     1501 tattcctgct ggaacttatg aggtagatct aatcgactct catcaatgcg acaccaacct
     1561 ttctttcact ctattagctc cgtcggattt tcaaatcata aaaaaaattt ctcactgttc
     1621 ctgtttcgga aacaatgatg gaagcatagc aatagaaatt gcaggaaacc agtccgacta
     1681 tcaaatactt tggggagacg gcagtaatga aaacatcagg cataacttct atgccggaat
     1741 ttatcctttt cgggtatccg ataaaaacga ttgtacaaag tatgatactg ctgtgataga
     1801 acaacccgag gccatcgaag tcatcaagag agttacaccc gtaggatgcg agagtgattc
     1861 ggacgggaaa atagaactga gcattggagg aggcagcccg cctttcacag tgatttggct
     1921 ggaaggtttt caaggggaaa tgttgtatga acttcccaaa ggaatttata cctttcaaat
     1981 cattgataac aacaattgct tagtaaagga cacagtcata gtgtggcaga aagattgctg
     2041 catcgatatt cccaatgtaa ttacccctaa cagcgatggt tttaatgata aatttgtaat
     2101 taaaggcatc gaaaacttcc ccagtaatga attgattata tacaaccgct ggggtaaaga
     2161 aatcattcag ttcaaaggtt ataaaaatga gtgggatggg aggaatgcaa atggtgagca
     2221 ggtggccgag ggggtttatt attacgtttt gcatcttact ggcggccgtt attttcaggg
     2281 atctatcact gtaatacgat aaagcaagcg gattgtggct ttggccgagc agagcgaagc
     2341 tcgcagcgag gtcaccaaaa taaataattc tggaattatc attagaattc ttatccttta
     2401 tgacaaattt ggacaatctt tcctctcgaa ataaaaaagt gattctcata taaaatttta
     2461 tttcttaatt ttgttgcagt attcatttga cttataaacc ctaaaattca aaatattatg
     2521 aaaaaaatat tagttgcaac cgaaaaaccc tttgcaaaag aagcaatagc agcgattcgc
     2581 aaggttgttg aagatgccgg atacacactg gctctcctcg agaaatatac ttcgaagact
     2641 gagttgtgca attcgatagc tgatgctgat gccttaatca ttcgcagcga tattgtcgac
     2701 aaagaagttg ttgaagctgc aaagaattta aaaattgtag ttcgtgccgg ggctggctat
     2761 gataatattg atctggaagc ctgcacaaaa catgggatag ttgctatgaa tactccagga
     2821 caaaactcga atgcagtagc cgagctggca tttggtatga tgttgtatat ggctcgggga
     2881 ggttttgacg gaggaaccgg caccgagctg cgtggaaaga cccttgggat tcacggatat
     2941 gggaatgtgg gaagatacat aggtaaaatc gctttaggct ttggaatgaa aacctatgct
     3001 ttccgacgta cctctatgga cgaaatcata aaagcagaag gaatcattcc cttatcaaaa
     3061 gctgaagaca tttacaaaaa ctgtcagtat gttacccttc atatcccggc caacgagcaa
     3121 acaaaaaaat caatcaatta tgatttgcta tcgctaatgc caaaaggagc cactttagtt
     3181 aacacagccc gcaaagaaat catcgacgaa gaaagccttt tgaaaatgtt tgccgaaagg
     3241 ccagatttca agtacctaag tgatattgag cccgcctgca aggctgagat catcgagaaa
     3301 tactccaaac gttacttttt tactccaaaa aagatgggtg ctcaaaccct cgaagccaac
     3361 aataatgcag gcatagccgc tgctcaacaa attatcgatt tcttcgaaaa gggtgacgtt
     3421 cgctttaagg taaattgatc cattccttca ttacatcata acaaaactaa agccgttctt
     3481 tgaaattggg taaaggtatt ccccgtattt tacggatgaa agttctataa atcctttcaa
     3541 atctgcatca tttactaccg aaaggttttg atacaataaa acagaactct cataagaaag
     3601 tgcctttaat catataacga caacatgaat tgaaatcctg aagccaaagg tttggaatca
     3661 ggaagttatc ctcgacagta tttcatagta ttcacttcat ttaagaattt acaccaatga
     3721 cagtttttag gtttcaagat ttgagagttt atcaaaaaac gctggagttc gcagagtggg
     3781 cttcacggat tgccgaagga cattcatcca atgggcaagg gcaaatcatc agcaacaagc
     3841 tacttgattc ggcagtgggt atagtttttt gcatagccga gggttcggct tcgccccgac
     3901 ctgttttcat cgaacaattg aaaaaggcaa agtctcatgt gagagaatgt atcgttatgt
     3961 cgcaactcat gttatctctt aaactgctaa atgaacgcaa ttacgagtat tgtcattcta
     4021 cactttctga aatcattaga atgataggag gactcattgc atccttgagc aaggaacagg
     4081 ctgatgcacc ccgagatgat cgagggaacg aacataaaaa caaagacacc ttataaattc
     4141 caactttgat ttactcgcta aactttacac acttatggct attttaaaac cattcaaagg
     4201 acttagacct ccaaaagaaa tcgcacatct tgtagctgcg cgaccttacg atgtactcaa
     4261 ttcaaatgaa gctcgtgagg aagccagagg aaacccttat tctttgctgc atatcacaaa
     4321 accggagatc gacctgccca tgggcaccga tgaacatgca ccggaagtat atcaaaaagc
     4381 ctctgaaaac ttccgcatgt tcaggcaaaa agactggctt gtgcccgacg agagggatct
     4441 tctatacatc tatgctcaaa ccatggacgg tcgtacacaa tatggaattg tgggatgcgc
     4501 cgccgttgac gattacttca atggcgttat caaacggcac gaactcacac gaaaagacaa
     4561 ggaagaagac cgaatgaaac acgtaatgat cacgaatgct aatctcgaac ccgtcttctt
     4621 tacttatcct gccgtaaatg aaatagataa aatcgtagac aaaatcgtaa aatcaaccga
     4681 gccagtttac gattttattg cccccgatgg aattggacat catttttggc ttatcgaaag
     4741 cgacgaaact attcaagaaa tagttcggct ttttgccaaa attccgaata cctacgtcgc
     4801 cgatgggcac caccgtacgg ctgctgctgc tctcgttgga gccgaaaaaa gaaaaatgaa
     4861 tcccaatcat agaggcgacg aagaatataa cttcttcatg gcagttcatt tccccgacaa
     4921 tcaactgaga atcatcgact acaaccgagt agtaaaagat ttgaacggtt tgtcgaaaga
     4981 agaattcatt gaaaaaatca aacaacattt tgtaattgcc gagaaaggcc agcaacctta
     5041 caaacctttt caccttcatg aattcagcat gtatctcgac ggaacatggt attccttaga
     5101 agccctcgga catacttaca atgagaatga tccaatcgaa gttctcgacg taaccattct
     5161 aagccgctat gttctcgacg aaatcttagg aatcaaggat ctgcgtaccg ataaacgcat
     5221 cgactttgtg ggaggcatca gaggaatgca ggaattgagc cggcgggtcg acagcggtga
     5281 aatggccgtc gcttttgcct tatatcccgt tacgctaaaa caacttattg ctatagccga
     5341 ttcaggtaag attatgcctc caaaaactac ctggttcgaa cctaaattac gctcaggcct
     5401 cgtagttcat ctgctcgact gatttttagc agaaatcctt gctcattatc ctaatttata
     5461 agtaactcat tcgatcttat aatacaacta atagagtcct aaccctccag tatgggctct
     5521 attttttatt cttattcatt atccctcctc gattctttat ggttattttt atgaatggaa
     5581 tatgaacctt tcaaacgttg tattgttaat tcattaacaa aagaaaatca tcaatatttc
     5641 ttttaatttt cagtaaaaag aatcagaaat caattaattt tagatttcaa ttttaaaaac
     5701 aggaggaaat atgaacagat tatcggataa agtagccata ataacgggag ctgccagagg
     5761 catgggcaaa gaagaagcca agttatttgc caaagaagga gccaaggtag ttgtggccga
     5821 catcattgaa aatgaagcca tagaagtagc caaagaaatc gccgagaatg ggggcgaagc
     5881 catggcatgt aaactcgacg tttccaatgc aaaaaactgg gaacgaatgg tagaggagat
     5941 aagcaataaa tggggcaaga ttgatattct tgtcaacaat g
//
LOCUS       DAOORU010000146         5955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_16197_length_5955_cov_5.216949, whole genome shotgun sequence.
ACCESSION   DAOORU010000146 DAOORU010000000
VERSION     DAOORU010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16197_length_5955_cov_5.216949"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..573)
                     /locus_tag="PLF20_08550"
     CDS             complement(88..573)
                     /locus_tag="PLF20_08550"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HOX81089.1"
                     /translation="MTRPNQVWAIDITYIPMARGFMYLTAIMDMYSRFILIWSISNTM
                     DSAWVTDVVKEAVKTYGKPEIINSDQGTQFTSDEYVDYVKSLESTKISMDGKGKAIDN
                     IFIERFWRTIKYDKIYIHCPKNGLELFQICEEFINYYNFERPHQTIDYQIPNNLYHEA
                     A"
     gene            complement(657..902)
                     /locus_tag="PLF20_08555"
     CDS             complement(657..902)
                     /locus_tag="PLF20_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81090.1"
                     /translation="MIDRNTRDLSMNEQCKLLNLHRSGIYYQPKSESTLDLEMMTQID
                     KLHLKDPTLGTRRMKVMLQMMGYKIGRTHVRTLMRKM"
     gene            complement(1009..1200)
                     /locus_tag="PLF20_08560"
     CDS             complement(1009..1200)
                     /locus_tag="PLF20_08560"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOX81091.1"
                     /translation="MKRTRRKFSAAFKLKVVLEALKEQSTLSELAHKYELHPNVISRW
                     KAEFLDNAESVFDSDTQKP"
     gene            1502..2311
                     /gene="ispE"
                     /locus_tag="PLF20_08565"
     CDS             1502..2311
                     /gene="ispE"
                     /locus_tag="PLF20_08565"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOX81092.1"
                     /translation="MIVFANAKINLGLRIIRKRSDNYHDILSVFYPIPLVDVIEIDQS
                     KKLEFYSYGLSITAPSESNLCVKAWNLLFENHVLTPVAIYLLKNIPTEAGLGGGSSDA
                     SFILKALNEMFHLGLSHETLSNYALQLGSDCPFFIHNKPMLVSGCGEVFTPVTLSLEG
                     LYLVLIQPNCKIHTAEAYSHIQPRHPAENPALIVNLPLERWREKLVNDFEDFVFEIFP
                     EVGIIKEKLYDEGAVYASMSGSGSSVYGIFRDEPPPMPWANKYFYRVFILD"
     gene            complement(2308..2676)
                     /locus_tag="PLF20_08570"
     CDS             complement(2308..2676)
                     /locus_tag="PLF20_08570"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HOX81093.1"
                     /translation="MNQRIYQYLPQIGLEEAMMIESFTQQLSDDEMRYFAGMYASRRR
                     DPLLILLLALIGFLGINGVHRFVIGQIGMGILYLLTGGLCLIGTIVDLVNHKQLALDY
                     NRKVASEILMMIKPNSTITA"
     gene            complement(2673..2993)
                     /locus_tag="PLF20_08575"
     CDS             complement(2673..2993)
                     /locus_tag="PLF20_08575"
                     /inference="COORDINATES: protein motif:HMM:NF022281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HOX81094.1"
                     /translation="MNKLKAFGQKGFIHYILSHLEGFIWIFALGGMMISPVTSEQHFT
                     FCPFYHLGFRHCPGCGIGRSMILALHGRIEESLMMHPLGLIAIIILIIRTIEIFSSKL
                     YYSL"
     gene            complement(3068..4081)
                     /gene="murB"
                     /locus_tag="PLF20_08580"
     CDS             complement(3068..4081)
                     /gene="murB"
                     /locus_tag="PLF20_08580"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOX81095.1"
                     /translation="MIIQQNISLKPFNTFQVEARAAFFTDVRQQSDIAEVLEFVHDNP
                     LPALILGSGSNFLFTSDYPGIVIHINTKGIECLGEENDKVYLKAEAGTYWEDLLDYAL
                     QEGFYGLENLTLIPGTVGSAPVQNIGAYGIELKDVFHSLEAIDLKSGLSRIFTLDECE
                     FGYRQSIFKSSQKGCYMITSVTFALSKYYNPCLDYKDLKEFFEGRLIDTITPHTLRDA
                     IRQIRLKKLPEVGKIGSAGSFFKNPVISENFFQKLLSEFPHVNGFKVDNGIKISAAWL
                     ISQCGWKGCRQGDAGVWPQQPLVLVNYGNASGKEILQLANKIIQSVEDRFAIHLEPEV
                     NII"
     gene            complement(4092..4862)
                     /locus_tag="PLF20_08585"
     CDS             complement(4092..4862)
                     /locus_tag="PLF20_08585"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HOX81096.1"
                     /translation="MKKQKSIVILFFTFVLSMMLDACTKEENGGCITSTGKIVYEERS
                     LLASPDTLIIEGEMDVYITMDRKPRLYFQSGENLLAGVVSTLIGKKLTLEDFNTCDFL
                     RNMGVRTKVFLTVTPQSFLFIEFESSGVISSTNTLSLDSIRIECRNGGGTVNLDIETA
                     KAWFIQHSGTGSVDFHIHGKSGYTFVYCMGYGPFDLRDLCTTGYLHFVHLGSNNCWVN
                     AGGILQAEIWSKGDLYYTGSPFTIWLKGEGSGRLIPFD"
     gene            complement(4867..>5955)
                     /locus_tag="PLF20_08590"
     CDS             complement(4867..>5955)
                     /locus_tag="PLF20_08590"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HOX81097.1"
                     /translation="FSFFTLGFLTILGFASRGQDILPIAGYKFQPVVHYGFIIPHHPE
                     MKVFTNHHITGLEVSLLKSSNGEKEWQLKFNYPVTGFSYFYSDLGNTGILGSVHAIYP
                     CIDFLLIRSRNTLLTIRLGAGLSWFTKTFHRIDNYKNVAIGSHINSAICSELSAQFGL
                     SSYLSLKMGASFLHFSNGAVKVPNYGINLPAIHIGLEGTLKKISIIPLAYQSQPVKFQ
                     PRLRVAMSGALKEIFPVGGKQYYALNLSLNGLTPVSRYFISGLGLDITWDQSDKELLA
                     RQGIYVDNEAEIINYGVNLFGGLRIGKLDLCLHAGTYLYRKEKSDNIIYDKIVASYTI
                     FEKMQLYLTLKTHFAKADYFAAGLGYQF"
BASE COUNT         1780 a         1205 c         1075 g         1895 t
ORIGIN      
        1 ggacaatatt tttatcaaag ttaatttatt atgttgaaaa gagggggtct ggggggagac
       61 tcataactca tttttttgtt aataattcta tgcggcttca tgatacaaat tattaggtat
      121 ttgatagtca attgtttgat gaggtctttc aaaattgtaa tagttaataa attcttcgca
      181 aatctggaat aattcaagcc cgttttttgg acaatgaatg tagattttat catatttgat
      241 tgtacgccag aatctttcga taaaaatgtt atcaatagct tttcctttac catccattga
      301 tatttttgtt gattccaaac tctttacata atcaacatac tcatcgctcg taaattgagt
      361 tccctggtcg gaattgataa tttctggttt gccgtaggtt tttacagctt cttttaccac
      421 atctgttacc catgctgaat ccatcgtgtt tgatatgctc cagattaaaa taaagcgact
      481 atacatatcc atgattgccg tcaaatacat aaaaccacga gccattggaa tgtaagtgat
      541 gtcaatagcc cagacctgat ttggacgagt aatattttat tgcctaacaa atatgggtat
      601 ttgtattttg caggatcaat gatagttgtc cggggccgac aataaatgac ttttatttac
      661 atttttctca tcagggttct gacatgtgtg cgtccaatct tatagcccat catctgaagc
      721 atgactttca ttcttcgtgt gcctaaagtg gggtctttga gatgtagctt atcaatttga
      781 gtcatcattt ccagatccaa agttgattca ctttttggct gatagtaaat gcctgaacga
      841 tgcaaattaa gcaatttaca ctgctcattc atgctcaaat ctctggtatt cctgtcaatc
      901 attgctctac gatattttaa aggaaaggac ctaacttttt tttaaccaat cattctcaat
      961 tttcatacgg ccaatttcct tgtaaagatc atcttctttc tgttgttctc atggcttttg
     1021 ggtatctgaa tcaaatacac tttctgcatt gtcgagaaat tcagccttcc atcggctgat
     1081 gacattggga tgtaattcat acttgtgagc caactcactc aaagtgctct gttcttttaa
     1141 tgcttccaaa actaccttga gtttgaatgc agctgaaaat tttcttcttg ttcttttcat
     1201 aatggacagt tttacgttaa tttttaaact tatgttgttg ttctattttt ttagaccatt
     1261 atatttatac ttttactgaa aattttgatg actgtatgga cagactctat ttattacatt
     1321 tttcaactat tttatttctc cttgcttcca gccagcgcca tcaatcaaga tcataataga
     1381 atcttcaggc atttaccccc attccccaat tctccgcgct tattgcgata tgctttcctt
     1441 aagacatgac atctttcgtg actattcata aaaagtttag ttttgccata aattgtatgc
     1501 aatgatcgtt ttcgccaacg caaagattaa tctgggttta cgcattatta ggaaaagatc
     1561 cgataactat catgatatct tgagcgtttt ttatcctatt ccactggttg atgttataga
     1621 gatcgatcaa agcaaaaaac tcgaatttta ttcttatgga ctttcaatta ctgctccctc
     1681 tgaatcgaat ctttgcgtaa aagcatggaa tctgcttttc gagaaccatg ttctgactcc
     1741 tgtggccatt tatctactca agaacattcc aacagaagcc ggactcggag gaggttcatc
     1801 cgatgcaagc tttatattaa aagccctgaa tgaaatgttt catttagggc tttcgcacga
     1861 aactctttcc aattatgcgc ttcaactggg cagcgactgt ccctttttca ttcacaataa
     1921 acctatgctg gtttcgggct gtggagaagt ctttactcca gttacacttt ctctcgaagg
     1981 gttatatctg gtattgattc aaccgaattg taaaatccat acagcggagg cttattcaca
     2041 tattcaaccc aggcacccgg ccgaaaatcc cgctcttatc gtgaatttgc ctttagaaag
     2101 atggagagaa aaattagtga atgattttga ggattttgtt ttcgaaatat ttcctgaagt
     2161 aggaatcatc aaagaaaaat tatacgatga aggcgcagtt tatgcatcga tgagcggtag
     2221 cggttcgagt gtttatggca tttttcgcga tgaacccccg cctatgccat gggctaataa
     2281 atatttctat cgtgttttca ttctcgacta ggctgttatg gtcgaattgg gctttatcat
     2341 catcaaaatt tccgaggcta ctttacggtt gtaatcaaga gcaagctgtt tgtgattgac
     2401 gagatcgaca atggtaccta taaggcataa acctcccgta agcaagtaca gaattcccat
     2461 gccgatttgt ccgatgacga aacgatgcac tccgttaatt cccaaaaatc caataagagc
     2521 taacagaaga atcagcaaag ggtctcttct tcgagacgca tacattcctg caaaatatcg
     2581 catttcgtcg tcggaaagtt gttgagtaaa actctcgatc atcatggctt cttcaaggcc
     2641 aatctgtggt aaatactggt aaattctctg gttcataatg aataatatag tttggatgaa
     2701 aaaatttcaa ttgtacggat gatgagaatg attatggcta tcaagcccaa aggatgcatc
     2761 ataagtgatt cttcgattct gccatgcagg gcaaggatca tacttcggcc tataccgcat
     2821 cctggacaat gcctgaaacc cagatgataa aatggacaaa aggtaaaatg ctgctcactt
     2881 gttacaggac ttatcatcat gccacctaag gcaaaaatcc agataaatcc ctccagatgc
     2941 gataaaatgt aatgaataaa ccctttttgt ccaaatgctt tcaatttatt cattctgtta
     3001 ttgatgtttt ttttgactaa aataaaaaca aaaggttaca tacggatgtc atcattaaaa
     3061 atttttttta aataatattg acttcgggtt ctaaatggat agcgaagcgg tcttcaacgc
     3121 tttgaatgat tttattggca agctgaagaa tttccttgcc tgaggcattt ccatagttca
     3181 ccagtactaa aggttgctgg ggccaaactc ctgcatcacc ctgtcggcat cctttccatc
     3241 cgcattgact gatcagccag gctgccgaaa tttttatgcc attgtcaacc ttaaaaccat
     3301 tgacatgagg aaattcagaa agcaatttct ggaagaaatt ttccgaaatt accggattct
     3361 tgaaaaaact tcctgcactt ccaatttttc ctacctcggg aagttttttt aaacgaattt
     3421 gtcgaatggc atctcgtaaa gtatgaggag taatggtatc aattaatcta ccttcgaaaa
     3481 attctttcaa atccttatag tcgagacaag gattataata cttcgataag gcaaaagtaa
     3541 ccgaagtaat catatagcat cccttttggc tcgatttaaa gatgctttgt ctgtagccaa
     3601 actcgcattc atcgagtgta aaaattctgc tgaggccgct cttcaaatct atggcttcga
     3661 gactatggaa tacatctttc aattctatac cataagctcc aatgttttgt acgggtgcac
     3721 tgccaactgt tcctggaatc agcgtaagat tctcaagtcc gtaaaatcct tcttgcaggg
     3781 cataatcgag caaatcttcc caataagttc cagcttcagc cttcaggtaa accttatcgt
     3841 tctcttcacc taaacattct attcccttgg tattaatatg aattactatt ccgggataat
     3901 ccgatgtaaa caaaaaatta cttccacttc ctaaaatcaa ggcaggcaat ggattgtcat
     3961 gaacaaattc gagtacttca gcaatatcgc tttgctgcct gacatcggta aaaaatgcgg
     4021 cacgggcttc gacctgaaaa gtgttgaaag gctttaatga aatattttgt tgaattatca
     4081 tacgaataaa attaatcgaa aggtataagc cggccgcttc cttcaccttt caaccatata
     4141 gtgaaaggag agcccgtata atataagtcg cctttgctcc agatttccgc ttgcagtatg
     4201 ccaccagcat tcacccagca attgttcgaa cctaaatgta caaaatgcaa ataacctgtg
     4261 gtgcacaaat cacgtaaatc gaaggggcca taacccatac aataaacgaa ggtatatccc
     4321 gatttcccat ggatatgaaa atcaaccgag cccgtgccgc tgtgctgaat aaaccatgct
     4381 ttcgcggttt ctatgtctaa atttacggtt cctcctccgt ttctgcattc tattcgtatt
     4441 gaatccaacg atagagtgtt ggtcgaagat atcacacctg aactttcaaa ttcgataaat
     4501 aggaatgatt gaggcgttac tgtaaggaat accttggttc taacgcccat attgcgcaaa
     4561 aaatcacaag tattgaagtc ttcgagggtt agttttttcc cgattagagt ggatactaca
     4621 ccagctaaca gattttctcc cgattgaaaa taaagccgtg gttttctgtc catagtaata
     4681 tatacgtcca tttctccttc gatgatcaat gtatcgggag atgcaagcaa ggaacgttct
     4741 tcataaacaa tctttcctgt cgaagtgata cagccgccat tctcttcctt tgtacaggca
     4801 tccaacatca tactgagtac gaaagtaaaa aataagataa cgatggattt ctgttttttc
     4861 attggtttaa aattgatagc ccaggccggc tgcaaaataa tcggctttgg caaagtgggt
     4921 tttcaaggtg agatacagtt gcattttttc aaagatagtg tatgaggcta ctattttgtc
     4981 gtagatgatg ttatccgatt tttcctttct gtagagataa gttcccgcat gcaaacaaag
     5041 atcgagttta ccaattcgca aaccgccgaa caaatttact ccataattga tgatttcggc
     5101 ttcattgtct acgtatattc cctgtcgtgc caataattcc ttatcagatt ggtcccaagt
     5161 aatatccagt ccaagcccgc tgataaaata tctcgaaaca ggtgtcaatc cgttcaacga
     5221 caaattcaat gcataatatt gttttccacc taccgggaaa atctctttca acgctcctga
     5281 catggctaca cgcaaacgag gctgaaactt aaccggttga gattgatagg ctaggggaat
     5341 gatggagatt tttttcaaag taccttccaa tcctatatgt atggctggaa ggtttattcc
     5401 gtaattcgga acttttactg ctccattgga aaaatgtaaa aatgaggctc ccattttaag
     5461 gctcaaataa gaagaaagtc cgaattgagc agaaagttca gaacaaatgg cggaatttat
     5521 atgagaacca atggctacat tcttataatt atctatccgg tgaaaagtct tggtaaacca
     5581 gctcaaacct gctcctaatc taatggtaag cagggtattc cggctcctga ttaagagaaa
     5641 atctatgcac ggataaatcg catgaacact gccaagaatt ccagtattgc ccaagtcgga
     5701 ataaaaataa ctaaacccgg taactgggta attgaattta agctgccatt ccttttcgcc
     5761 attgctcgac ttcaacaaac taacttccaa acctgtaata tgatgattgg taaatacttt
     5821 catctctgga tgatgaggaa taataaatcc ataatgcacc acgggctgaa acttatagcc
     5881 tgcgataggt aaaatatcct gacctctgct tgcaaagccc aaaatcgtca aaaatcctaa
     5941 agtgaagaaa ctgaa
//
LOCUS       DAOORU010000147         5925 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_16303_length_5925_cov_5.578194, whole genome shotgun sequence.
ACCESSION   DAOORU010000147 DAOORU010000000
VERSION     DAOORU010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16303_length_5925_cov_5.578194"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="PLF20_08595"
     CDS             complement(<1..553)
                     /locus_tag="PLF20_08595"
                     /inference="COORDINATES: protein motif:HMM:NF015662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81098.1"
                     /translation="MESFKKKIVWRLWFIYWLMFLLGMVILGKVIYIQAVKGKEYREM
                     VKQSTFKFDEIEAIRGNILAADGSLLATTVPIFDVRMDVANPNISKEFFNKKVDSLAL
                     GLSKLFPNKSRNEWKSELKEARKQGNRYYLIKRNVDYSTLKRIKTLPILCEGKNKGGL
                     ILIQKNIRKMPYGMLARRTIGYEN"
     gene            complement(560..940)
                     /locus_tag="PLF20_08600"
     CDS             complement(560..940)
                     /locus_tag="PLF20_08600"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HOX81099.1"
                     /translation="MKKTNSHIKSKKKHRNSVKSWISGAIVINKEIPIIKVVGFLAFI
                     GLIYIASNYHAMESLRRINQLKTDIKELRYEYINAKSQFTDSTKQSRMIYRASALGLR
                     PSQDPPIRIAVCDEDFKELKKLKQ"
     gene            complement(937..1833)
                     /gene="rsmH"
                     /locus_tag="PLF20_08605"
     CDS             complement(937..1833)
                     /gene="rsmH"
                     /locus_tag="PLF20_08605"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOX81100.1"
                     /translation="MSYHLPVLLAESIAALEVGKGGVFVDATFGGGGHTAEILRQMPS
                     GRVIAFDQDSDVIANLPKDERLVFINDNFRYLKNFLRFYEAIPVDGILADLGVSSYQI
                     DRAAKGFSTRWDGPLDMRMDKRKSLTAAQIINKYSETELFRLFKQYGELAQARRIASI
                     ICNERTRMSLETTTQLCELIKPLAPKGAENKFMAQVFQALRIEVNDELEVLKEFLLQS
                     IDVLCPGGRIAIISYHSLEDRLVKNFFKSGNFEGEVHKDFFGNVVCPLRPVNKKPIEP
                     SPTEIVDNPRARSAKLRIAERL"
     gene            complement(1852..2310)
                     /gene="mraZ"
                     /locus_tag="PLF20_08610"
     CDS             complement(1852..2310)
                     /gene="mraZ"
                     /locus_tag="PLF20_08610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOX81101.1"
                     /translation="MITFIGTHECKLDGKGRIQLPISLRKQLPGDGIQEFVIRRSIFS
                     KCLELYPKSEWDHELEKINKLSRYEPENADFIRRFMAGSKSIELDSSGRILIAKDLLN
                     YAEINKDVVIASQLNILEIWDKEKYEQTVNDPNIDFATLAQEVMKNIDKE"
     gene            2572..3447
                     /locus_tag="PLF20_08615"
     CDS             2572..3447
                     /locus_tag="PLF20_08615"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HOX81102.1"
                     /translation="MENQAEDISLNAIQPKKIVDLDEVIRQKSARVYRMLPRFVVHWL
                     EKIIHQDGLNYILTKYEDQEGLEFIESVLNEFGLRVDSDGAMGWDPKGRYIFVANHPL
                     GGMDGMAFMMVVGRYFPEVVFPVNDFLLYLPNIRNLFIPINKHGRNPELIKLLDDTFA
                     SDKTILYFPAGLCSRSQHGEILDLEWKKTFVTKARLHQRDIVPVHITGRNSAFFYRLA
                     NLRKSLHIHFNFEMILLVDEMFKQKGKSLLMRFGPVIPYTQLDRRFSDYEWANLIRAY
                     IYRLGKGDLNPFQPI"
     gene            3525..4502
                     /locus_tag="PLF20_08620"
     CDS             3525..4502
                     /locus_tag="PLF20_08620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOX81103.1"
                     /translation="MKPIIPPVDKRLIERELTESKFLRNTNYGKRQIFIVTYHDSPNI
                     IREIGRLRELSFRDAGGGTGNELDIDVYDTAGVPFKQLIVWDPYDEEIIGGYRYIQMK
                     NLPRLPSGQLDSPTARLFEFSNRFIDEFVPVTIELGRSFIQPSYQATFNVRKGMFSLD
                     NLWDGLGALVIEHPSIKYFFGKMTMYRNYNPRARDILLHFLQKFFPDPDHLVWPHSSL
                     EVKPEDVDIDSLFNGSTYEDNYRLLNQAIRKFNENIPPLVNAYMNLSPTMRYFGTAIN
                     HKFGEVDEGGILITIQDVYEKKKRRHLATYIPKFQLPKFKLHISRRREE"
     gene            4492..5091
                     /gene="yihA"
                     /locus_tag="PLF20_08625"
     CDS             4492..5091
                     /gene="yihA"
                     /locus_tag="PLF20_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOX81104.1"
                     /translation="MKNKLEAVFLKSSVTPSQFPPPTLPEYAFTGRSNVGKSTLINML
                     LQCRGLAKTSSTPGKTRTINHYLVNRSWYLADLPGYGYARLSKIERKALSNMISSYLQ
                     SRKNLLNTFILIDARISPQPIDIDFINIFGEYQLPFCLVFTKTEKLGQMVLQQQIDAF
                     LETLSPFWDEFPKYFITSGKTKVGRDEILDFINDTNLIM"
     gene            complement(5181..5558)
                     /locus_tag="PLF20_08630"
     CDS             complement(5181..5558)
                     /locus_tag="PLF20_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOX81105.1"
                     /translation="MNEENLPIPSEELPPIEMVKVNVDGSTFLTTLSPKFKLSKPYKI
                     NEPDKITAFIPGIIINVLVKKNQVVRKGDKLLILMAMKMNNHIRAPFDAIVKKIYVKK
                     GDQVKKDQLLVELQPILELNSIN"
     gene            complement(5590..>5925)
                     /locus_tag="PLF20_08635"
     CDS             complement(5590..>5925)
                     /locus_tag="PLF20_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOX81106.1"
                     /translation="GGYIAMCSHHLKADFVLAWPTAEIAVMGPEGAANIIFRKEIMEA
                     ENPEEMRKQKIEEYKAKFANPYVAAAYGYVDAVIEPSETRHFLTHALEISVSKSETLP
                     RKKHGIPPF"
BASE COUNT         1654 a         1200 c         1165 g         1906 t
ORIGIN      
        1 tattttcgta gccaatagta cgacgagcaa gcatgccata aggcattttt ctgatgtttt
       61 tctgtatcaa aatgagacca cccttgtttt tgccttcaca taaaataggc agggtcttga
      121 ttctttttag agtggaataa tctacattgc gcttgatcaa ataatatcga ttgccttgtt
      181 tccgggcttc ttttagctct gatttccatt cattcctgct tttattgggg aataatttcg
      241 ataggcccaa cgccagacta tcaactttct tattgaaaaa ttctttcgaa atatttggat
      301 tagccacatc catacgaaca tcgaaaatag gtacagtagt cgccagcagg ctcccatcag
      361 cagcaagaat atttcctcta atggcttcaa tttcatcgaa cttgaaggta gattgtttaa
      421 ccatttcacg atattccttg ccctttactg cctgaatata gatgacctta cccaatatta
      481 ccatacccaa gagaaacatt agccaatata tgaaccatag acgccagact atttttttct
      541 tgaatgattc catatctgac tattgtttaa gttttttcaa ctctttaaaa tcttcatcac
      601 atactgctat tcttatagga ggatcttggg agggtcgtag accgagtgcc gaagcgcgat
      661 agatcattct cgactgcttg gtcgaatcgg taaattgcga ctttgcattg atatattcgt
      721 accttagttc tttgatatcc gtcttaagct gattgattct tctaagtgat tccatcgcat
      781 gatagttgga agcgatatag atcaatccaa tgaatgctaa aaagccaacc actttaatga
      841 tggggatttc cttattgatg actatagctc ccgaaatcca gctttttaca ctattcctgt
      901 gctttttctt cgatttgata tgtgaattgg ttttcttcat aatcgttcag caattctaag
      961 tttagcactt ctggctctcg gattgtcgac gatttcggtg ggcgaaggtt cgatgggttt
     1021 tttgtttaca ggccttaacg gacaaacaac atttccaaaa aagtctttat gaacttcacc
     1081 ctcgaaattc ccgctcttaa agaagttttt taccaaacgg tcttcgagcg aatgatagga
     1141 aatgatggcg atgcgaccac ctgggcataa tacatcgata gattgaagaa gaaattcttt
     1201 gagtacttct aattcatcgt ttacttctat gcgcagagcc tgaaatacct gggccatgaa
     1261 tttgttttcg gctccttttg gggcaagagg ttttattaat tcacacaact gagtggtggt
     1321 ttccagagac attctggtgc gttcgttaca gatgattgaa gcaatgcgac gagcttgcgc
     1381 caattcgcca tattgcttaa acaacctgaa gagttcggtt tctgaatatt tattgatgat
     1441 ctgggcggct gtgaggcttt ttcttttatc catgcgcata tcgaggggtc catcccatcg
     1501 ggtcgaaaaa ccctttgccg ctcggtcgat ctgatatgat gaaaccccta aatcggccaa
     1561 aatcccatct acaggaatag cctcgtaaaa tctcaagaaa ttcttcaaat aacgaaagtt
     1621 atcatttata aaaaccaaac gttcatcttt gggtagatta gcaataacat cgctatcctg
     1681 atcgaaagct atgacgcggc ctgacggcat ttgccgcaaa atttcagctg tgtggccgcc
     1741 tcccccaaaa gttgcatcaa caaacacacc tcctttcccc acctccaagg cagcaatgct
     1801 ttcagcaaga agtacaggaa gatgatagct catatgatct taattatttt gtcattcctt
     1861 atcgatgttt ttcataactt cctgggctaa agtcgcaaaa tcaatattcg gatcgtttac
     1921 ggtttgttca tatttttctt tgtcccagat ctcaagaata ttcaattgcg aggcgatgac
     1981 tacatccttg ttgatttcgg cgtagttgag taagtctttt gcaatcaaaa ttcgacccga
     2041 ggagtccaat tctatagatt tgctccccgc catgaaccga cgtataaaat cagcattctc
     2101 aggctcatat ctacttaatt tgttgatttt ttcgagctca tgatcccatt ccgactttgg
     2161 atataattcc agacatttgc tgaaaatact gcgacggatg acaaattcct ggatgccatc
     2221 gccgggaagc tgcttgcgca aacttatggg aagctgtatg cgccccttgc catcgagttt
     2281 acattcgtgt gtgccaataa aggttatcat cttcagcaaa tcttactaca aaaatacaat
     2341 aatttcccac aatattacac aaaaatacaa aatttcacac aaaatttttc cacaattgtt
     2401 aataactttt tttaaaggct tatttttttg ggaaatccca tgaattaaaa tcaagctgaa
     2461 acccttgtat agagatattt ttgtgcagat ggaaaacaaa atcagatact tctttaaatc
     2521 agctttgata gtgatttttt tatctttgca tgagaaaagt cttggataaa aatggaaaat
     2581 caggcggagg acatatccct taacgctata caacccaaaa aaatcgttga tctcgatgaa
     2641 gtgatccggc aaaaaagtgc gagagtttat cgtatgttac cacgatttgt cgttcattgg
     2701 ctcgagaaaa tcattcatca agacggactc aattatattt tgaccaaata tgaggatcaa
     2761 gaggggctcg aattcatcga gtcggttttg aatgaatttg gtttacgtgt ggattcggat
     2821 ggtgcaatgg gttgggatcc taaaggccgt tatatttttg ttgctaatca tcctcttgga
     2881 ggtatggatg gtatggcttt catgatggtt gtgggacgtt attttcccga agtagttttc
     2941 cccgtgaatg attttttact gtatttaccc aacattcgca atttatttat cccaatcaac
     3001 aagcatggac gcaatcctga gttgatcaag ctgctggatg atacttttgc cagtgataaa
     3061 acaatactct attttcctgc gggtttgtgt tcgcgtagcc agcacggaga gattctcgac
     3121 ctcgaatgga aaaaaacctt tgtgactaaa gcaaggcttc atcaacgaga tattgttcct
     3181 gtgcatatta cgggtagaaa ttcggctttt ttttaccggc ttgccaatct tcggaaatcc
     3241 ttacatattc actttaactt tgagatgatt ctgttggtgg atgaaatgtt taaacaaaag
     3301 ggcaaatcgc tgttgatgcg ttttggcccg gttattccct atacccaatt ggaccggcgt
     3361 ttttccgatt atgaatgggc aaatcttatc agggcttata tctatcggtt aggcaagggt
     3421 gatcttaatc cttttcagcc aatatgatcg ttctttcatt tgattttttt ctgagatgat
     3481 ttcgaaagac aaaccgattt atcttacaat ctgaaaatat ggaaatgaag cctattatac
     3541 cgcctgttga taagagacta attgaacggg aacttacaga atcgaaattc ctgaggaata
     3601 ccaattatgg gaaacgtcag atatttatag tgacttatca tgactctccc aatatcatac
     3661 gtgagattgg gagattgcgc gaactttcct ttcgtgatgc gggtggcggt actggaaatg
     3721 aacttgatat cgacgtttat gacactgcgg gggttccttt caagcagctg attgtttggg
     3781 atccttatga tgaagaaatt atcggaggtt ataggtatat tcaaatgaag aatcttccgc
     3841 ggcttccttc tgggcaactc gattccccta cagctcgtct gttcgagttc tcgaaccggt
     3901 ttatcgatga atttgtgccg gttaccattg aacttgggcg ctcgttcatt cagccttcct
     3961 atcaggcaac tttcaatgta agaaaaggca tgttttcttt agataatctt tgggatggtt
     4021 tgggcgcact ggtaatcgaa catcctagca taaagtattt ctttggtaag atgacgatgt
     4081 atagaaatta taaccctcga gcaagagata ttctattgca ttttcttcaa aagttcttcc
     4141 ctgatcccga ccatctggta tggccgcact cttccttgga agtgaagccc gaagatgtgg
     4201 atatcgactc acttttcaac ggttctactt atgaagataa ctatcgtttg ctgaatcagg
     4261 ccatccgtaa attcaacgaa aatatacccc ctctggtcaa tgcctatatg aatctcagcc
     4321 caaccatgcg ctattttggt acagctatca accataagtt tggtgaagtt gacgaaggag
     4381 gtatcctcat taccattcaa gatgtgtacg aaaaaaagaa gaggcgtcat ttagcaactt
     4441 atattcctaa atttcagctt cccaaattta aacttcatat ttcgcgccgt cgtgaagaat
     4501 aagttggaag ccgttttttt gaaaagcagc gtcacccctt cgcaattccc tcccccaacc
     4561 ctgcctgaat atgcctttac cggaagatcg aatgtaggca aatctaccct tatcaatatg
     4621 ttgctccagt gcagaggttt ggccaaaact tcttcaaccc cgggcaaaac tcgcactatc
     4681 aatcattatc tggtgaatcg ttcatggtat ctcgccgatt tacctggcta cggttatgct
     4741 cgattatcga aaatagaaag gaaagcactc agcaacatga tatcatccta tctccaatct
     4801 cgaaaaaatc tgctcaatac attcattctt atcgatgctc gaatttcacc ccagcctata
     4861 gatattgatt ttatcaatat tttcggtgaa tatcaattgc ctttttgcct tgtatttaca
     4921 aaaaccgaaa aattgggaca aatggtttta cagcagcaaa tcgatgcatt tttagaaact
     4981 ttgtcacctt tctgggatga atttcccaag tatttcatta cctcggggaa aactaaggtt
     5041 ggtcgcgacg aaatccttga ttttatcaac gacacaaacc tcattatgta gcctgcaaaa
     5101 aaaaaatcct cgaaaaaact aaatttccgg gggttcgcat gaagttcatt tggaaaatgg
     5161 gcgatgggga ggttaagcag ttaattaata gaattcaatt caaggatggg ctgaagttca
     5221 acgagaagtt ggtctttttt tacctgatcg ccttttttga cgtaaatttt tttcactatg
     5281 gcatcgaagg gggcacggat atgattattc attttcatgg ccataagaat caataattta
     5341 tctcctttcc gtacgacttg attctttttt actagcacat tgatgattat accgggaatg
     5401 aaggccgtaa tcttatccgg ctcatttatc ttgtaaggtt tactaagttt aaacttagga
     5461 cttaaggtgg taagaaaggt cgatccgtca acattgactt ttaccatttc tatgggaggt
     5521 aattcttctg atggaatggg taaattttct tcgttcatgg gctattgttt taattcaacc
     5581 aataattaat taaaagggag ggattccgtg ctttttgcgt ggtagggttt ctgattttga
     5641 gacagatatt tcgagtgcat gagttaggaa atggcgagtt tcgctaggtt cgattacggc
     5701 atccacatag ccataagctg cggctacgta tggattggca aattttgcct tgtattcttc
     5761 gattttttgt ttgcgcattt cctcgggatt ttcagcttcc atgatttctt tacggaagat
     5821 gatgttagcc gctccttcag gacccataac ggcaatttct gcagtaggcc atgctaaaac
     5881 aaagtctgcc ttcaaatggt gagaacacat ggcaatatat cctcc
//
LOCUS       DAOORU010000148         5923 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_16311_length_5923_cov_5.739264, whole genome shotgun sequence.
ACCESSION   DAOORU010000148 DAOORU010000000
VERSION     DAOORU010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5923
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16311_length_5923_cov_5.739264"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="PLF20_08640"
     CDS             <1..444
                     /locus_tag="PLF20_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOX81107.1"
                     /translation="EEQWDKVMAVNAKGNFLGVKYVLPVMKKAQKGSIINISSIYGLI
                     GSGAAAAYHASKGAVRLFTKTIAAELAKYNIRVNSIHPGVIRTAMTDELLKDPANTKN
                     LLGTTILGRPAEAIEVAYGALFLASDESSYMTGSELVIDGGYTAL"
     gene            complement(775..1341)
                     /locus_tag="PLF20_08645"
     CDS             complement(775..1341)
                     /locus_tag="PLF20_08645"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOX81108.1"
                     /translation="MAGFRNVILFLCILFLFPSICSAQQDTVVRVRPSRSPRTATILS
                     LVLPGMGQAYNHKYWKIPIIYTGFAATGYFAIINGDEYHKFKDAYNFKINNLTGDPPN
                     DYAVKYTADQLRVQRDYYRRNMELSYIIAGFIYILNAVDASVDAHLSSFDISENLSMG
                     LIAPSPPIANSSPFISPSFGFTYKIPLR"
     gene            complement(1364..2269)
                     /locus_tag="PLF20_08650"
     CDS             complement(1364..2269)
                     /locus_tag="PLF20_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOX81109.1"
                     /translation="MTTNKKSALGRGLSAILQSPETDITSRDISGEFVAGAIAEIEID
                     KIEANPFQPRSEFEEASLNELAESIHQQGIIQPITVRKMGYDKYQLITGERRLRASKM
                     AGLEKIPAYIRIANDEEMLEMALVENIHRDDLNPLEIAMSYQRLIEEVGIPVNDVASK
                     VGQNRSTVVNYTRLLKLPPEVQIAIRDVQISMGHARAIINVEDIEDQLIILKEIIDHD
                     LSVRQVEQWVRDLKKAQVSQETEIKKLPNEKPVLPDSYRSVKDHLAEKFGSEVQINRN
                     AKGKGQIIIRFDNDEEFRKIVSLLQ"
     gene            complement(2259..3074)
                     /locus_tag="PLF20_08655"
     CDS             complement(2259..3074)
                     /locus_tag="PLF20_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOX81110.1"
                     /translation="MGKIIAIANQKGGVGKTTTAINLGASLAVLEFKTLLVDADPQAN
                     ATSGLGFDPRNITSSIYECIIGDIDAAKIILTTETPNLDLIPAHIDLVGAEIEMINLP
                     NREKMMRKVLSPIKDSYDFIFIDCSPSLGLITVNALVAADSVIIPVQCEYFALEGLGK
                     LLNTIKIVQSRLNTNLDIEGILLTMYDGRLRLARQVVEEVKMHFQTMVFDTIINRNTR
                     LSEAPSFGQSIIMFDADCVGTANHLNLAREILQKNNLTKHSPLNQNNLNFHDN"
     gene            complement(3204..3536)
                     /locus_tag="PLF20_08660"
     CDS             complement(3204..3536)
                     /locus_tag="PLF20_08660"
                     /inference="COORDINATES: protein motif:HMM:NF023109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HOX81111.1"
                     /translation="MMNEREKTNKGYLDLFSRTIIARNRRYYLDVKSSENNLVLTLTE
                     SKRKFNNRNGKFYYERNRVIVFSEDMDNFMNSFREVEKFLKDYLKRHPDISNFPSPPN
                     AFGSKDSP"
     gene            3714..4838
                     /gene="nusB"
                     /locus_tag="PLF20_08665"
     CDS             3714..4838
                     /gene="nusB"
                     /locus_tag="PLF20_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOX81112.1"
                     /translation="MLTRRHLRIKVLQALYAYFQSDNHEIDLAEKQLFSSIQHIYDLA
                     IYQISFLLNLSRFAEERLEDAKLKFRPNEEDLNPNTRFIENAFLKKLESNQSLMKHIQ
                     KIKISWTEYEDVTRRIYKTLRSSKEYQEYMAKSQISFREDKDFIVHIFNRLIVSDIGL
                     QAIFEEKNIFWTNDIHEETILLNKEILASENSDHLADREILLKIYRKEGNENEDIEVV
                     CHEQSIYEANDYSIAAWLANRILNHLKEKLDESAPLPPLLKTSDITEENDREFLLGLF
                     RKTILHDEDYDEYISEKIINWDLERIAIMDAIILKMAITEILEFPYIPIKVSMNEYIE
                     LAKIYSTPRSSQFINGMLDKIVDSLKEKNLIKKSGRGLLE"
     gene            complement(4855..5490)
                     /locus_tag="PLF20_08670"
     CDS             complement(4855..5490)
                     /locus_tag="PLF20_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018361091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HOX81113.1"
                     /translation="MKKKYKYYVIWRGTQPGVVDSWEECLERVKGFQGAFYKGFYDEE
                     SAREAFAQSPEKYIKPSEPSREDEDHKEIVSSEIILPSICVDAACSGNPGLLEYQGVD
                     TASRRQLFHQGPFKDGTNNIGEFLAIVHALAMCKKKGLNLPIYSDSRNAMQWIKNRKA
                     KTKLTENENNKYLFELIIRAENWLQNNEWDNQILKWDTEQWGENPADFGRK"
     gene            complement(5657..>5923)
                     /locus_tag="PLF20_08675"
     CDS             complement(5657..>5923)
                     /locus_tag="PLF20_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81114.1"
                     /translation="KEKEKEKEKEKEKEKEKETKIAEHTKLIYYGNDTGFDKLSPVWP
                     PIIHRSSSTPSGCIEITDLSNFLIKICGYNSSLGTFRYFSMRLS"
BASE COUNT         1748 a         1194 c         1190 g         1791 t
ORIGIN      
        1 gaagaacaat gggacaaggt aatggccgtc aatgccaaag gcaatttcct gggagtaaag
       61 tatgtattgc ctgtcatgaa aaaagctcaa aaaggctcta ttatcaatat ttcatctatt
      121 tatgggctga ttggttctgg agctgctgca gcttaccatg cttcgaaagg agcagttcgg
      181 ttatttacca aaactattgc cgccgaatta gccaaataca acatccgtgt gaattccatt
      241 catccgggag tgattagaac agccatgacc gatgaattac tgaaggaccc ggcaaatacc
      301 aagaatcttc tcggaaccac catattagga aggcctgcag aagctatcga agtagcttat
      361 ggcgccttat tcctcgcttc agatgaatca tcttacatga ccggctctga attggtaatc
      421 gacggtggtt acaccgctct gtaaaattta ggggcaatga gaaagttatg ctctacaact
      481 ctcatattgc ccgttccacc taatcgatgc aataacctca ggatggcagt gtctgtctac
      541 taatctggcc gattcgtttc aatcaatcta gatggaattt cgaacccctg ggtaaatgtc
      601 atacttctaa tatacaggcc taaaaatacc atctttcaga aatccgcaca cttcttttta
      661 aaaacagata aaccgtttgt cgaaatagtt agcagttgat ttcctatatc gacaaatggc
      721 aaaacatagg aatacgaacc aaaatcaaaa ggttttgaaa acctgatttc tgttttatcg
      781 cagaggaatt ttgtaagtaa atccgaaaga aggagaaata aagggagaac tattggcaat
      841 aggaggagaa ggcgcaataa ggcccatgct caaattttca gaaatatcga aagatgaaag
      901 gtgagcatct acactagcat ctacggcatt taaaatatag atgaatccgg cgataatgta
      961 actgagttcc atgtttcgac ggtaataatc tctttgaacg cgtagctgat cggcagtata
     1021 tttcaccgca taatcgttgg gtgggtcgcc tgtaaggttg ttaatcttga agttataggc
     1081 atctttaaac ttgtgatatt catctccatt gatgatggca aaatatccgg ttgctgcaaa
     1141 gccagtgtat atgatgggaa ttttccagta cttatgatta taagcctgtc ccatacccgg
     1201 taaaaccaac gataatatgg tagccgttcg gggagagcgt gatggtcgaa ctcgaacaac
     1261 agtatcctgc tgggctgagc atatagaagg gaataaaaac aaaatacaaa ggaagagaat
     1321 tacatttctg aatcctgcca tagaaagaaa gtttggcatg gagttactgc aggagcgaaa
     1381 cgattttccg aaactcctcg tcgttgtcga atcggatgat gatttgtccc ttgcctttag
     1441 cgttacggtt tatttgaacc tcagatccaa acttttcggc aagatggtct ttgacagatc
     1501 gatatgaatc gggcaggaca ggtttttcgt tgggtaattt tttaatttcc gtttcctgac
     1561 ttacctgggc ttttttcaaa tcgcgcaccc attgttcaac ctggcggaca gaaaggtcgt
     1621 gatcgataat ttctttcaga attatcaatt gatcttcgat atcttcgacg ttgatgattg
     1681 cccgagcgtg tcccatgctg atttggacat cgcgaatggc tatttgaacc tcgggaggaa
     1741 gttttaacag gcgggtataa ttaacgaccg tggagcggtt ttgacctacc ttgctggcca
     1801 catcattgac aggaattccc acttcttcga tgagtcgctg atagctcatg gctatttcca
     1861 gtgggttcag gtcgtcgcga tgaatatttt ccacgagtgc catttcgagc atttcctcgt
     1921 cattggcaat acggatgtaa gcaggtatct tttcgaggcc agccatctta gaagctctaa
     1981 gcctgcgctc gcctgtaatc aattgatact tatcgtatcc catttttctg acggtaatgg
     2041 gttgaatgat gccctgctgg tgaatggatt cggccaattc gttcaaagag gcttcttcga
     2101 attcagatct tggttgaaaa ggattagctt cgattttatc aatttcgatt tcggctatgg
     2161 ctcctgccac aaattctccc gaaatatctc tcgaagtaat atcggtttcg ggactctgca
     2221 atattgcgct cagccctcgc cccaaggccg atttcttatt agttgtcatg gaaattcaag
     2281 ttattttgat ttaatgggga atgtttggta aggttatttt tctgcagaat ttctctggcc
     2341 agattcagat ggtttgcagt tccaacgcaa tcggcatcaa acatgatgat tgactggccg
     2401 aaactgggag cttcgctcaa gcgtgtattt ctattgatga tggtgtcgaa gaccatggtt
     2461 tgaaagtgca tctttacttc ttcgacaacc tgccgagcaa gcctcaaacg gccatcatac
     2521 atggtaagca atatcccttc aatgtcgaga ttggtgttaa ggcgcgattg aacgattttt
     2581 atggtattca gaagctttcc gaggccctcg agggcaaaat attcgcattg aaccgggata
     2641 atgaccgaat cggcagctac caatgcattc accgtaatca aacccagcga aggagaacaa
     2701 tcgataaaga taaagtcata agaatcttta atgggagaca gaactttccg catcattttt
     2761 tctctgttgg gcaggttgat catttcgatt tctgcaccca ccagatcgat atgtgcaggg
     2821 atgagatcga gattgggggt ttcggttgtc aaaatgatct tagccgcatc gatatcgcca
     2881 ataatgcatt cgtatatgct agaagtaata ttacgaggat cgaaaccaag gccagaggtg
     2941 gcatttgcct gagggtcagc atctaccaat aaagttttga attccaatac ggccaggctg
     3001 gctcccagat tgatcgccgt cgtcgttttg cctacaccac ctttttggtt cgcaattgcg
     3061 ataattttac ccataaaaat atattcgaga aatggaactt tccatcagca aaattaaaca
     3121 aatatatgtc tgctttacct aaaattaggc tatcctatcg gctgattcaa tgtaacaaac
     3181 tgattgataa tttatttact tgttcagggg gaatcttttg agccgaaagc attcggtgga
     3241 gaagggaaat tcgaaatgtc aggatgtcgt ttgagataat ccttcagaaa tttctccact
     3301 tcccgaaaag aattcatgaa attatccata tcttcggaaa atacgataac cctattgcgc
     3361 tcataataaa acttgccatt gcggttgttg aacttgcgtt tgctctcggt caaagtcaaa
     3421 acaagattgt tttcgctgga ttttacatcg aggtaatatc gtctattcct tgctatgatg
     3481 gttcttgaaa ataaatctaa gtaacctttg tttgtttttt ctctttcatt catcatttta
     3541 attacttata attttaatga gacaaaaata gtgggatgaa tcccgacatg caaaattttc
     3601 cattaaaaat ttagattaag atttagaaac agaaattccg aggttctcga ttgttgtgag
     3661 ccacttgata atgatgtatt tttgcagccg taaaaaaatg ctccttctca aacatgctta
     3721 cacgacgtca tcttagaatc aaggttttgc aagctttata tgcctatttc cagtctgata
     3781 atcatgagat agaccttgct gagaagcaat tattcagcag catccagcat atttacgatc
     3841 ttgctattta tcaaatttct ttcttattga atttatcgag atttgctgaa gaacgtctgg
     3901 aagatgccaa attaaaattc agacccaatg aggaagattt gaaccccaat actcgtttta
     3961 tcgaaaatgc tttcttaaaa aaactcgaga gcaatcagag cctgatgaag catattcaga
     4021 agataaaaat ttcatggaca gagtatgaag atgttaccag aagaatttac aaaaccttga
     4081 gatcgtcgaa agaatatcag gaatatatgg caaaatctca aatcagtttt cgtgaggata
     4141 aagatttcat tgttcatata tttaatcggt tgattgtttc tgatattgga ttgcaagcca
     4201 ttttcgaaga aaagaacatt ttctggacca atgatatcca cgaagagacc attttactca
     4261 acaaagaaat tctggcttcg gaaaattccg atcatctggc cgatagagaa atacttctga
     4321 aaatttatcg taaggaaggg aatgaaaacg aggacattga ggtggtatgc catgaacaaa
     4381 gtatttacga agccaatgat tattccatag ctgcatggct tgccaatcgc attctaaatc
     4441 atttaaaaga gaaattggac gaatcggctc cgcttccacc cttgctgaaa acttctgaca
     4501 ttaccgagga aaacgacagg gagttcctgt tgggtctttt tcgaaaaaca attcttcatg
     4561 atgaagatta cgatgaatat attagtgaaa agattatcaa ctgggatctc gaacgaattg
     4621 ccatcatgga tgccataata ttgaaaatgg ctattaccga aatcctcgaa ttcccctata
     4681 ttccaatcaa ggtgagtatg aacgaataca ttgaactggc aaaaatctac agtacacctc
     4741 gcagcagcca gtttatcaat gggatgctcg ataagatcgt cgattcgctg aaggaaaaga
     4801 atctgataaa aaaatcaggc cggggacttt tggagtaaac cttcaggagg ccttctactt
     4861 tcgaccgaaa tcggctggat tttctcccca ttgttcggta tcccatttca agatttggtt
     4921 atcccattcg ttgttttgca gccaattttc ggcgcgtatt ataagctcga acaaatattt
     4981 attgttttca ttttcagtca atttagtttt agcttttcga ttctttatcc attgcatggc
     5041 attgcgggaa tcggagtaaa tgggcaggtt caaacccttc ttcttgcaca ttgccaaggc
     5101 atgaacaata gcaaggaact ccccaatatt attggttcca tccttgaaag gcccttgatg
     5161 gaaaagctgt ctccggcttg cagtatccac accttggtat tcgagaagcc ccggattacc
     5221 cgaacaagcc gcatcgacac aaatgctcgg taaaataatc tcagagctga caatttcttt
     5281 atgatcttcg tcctctcgag atggctcact cggtttgata tatttttcgg gggattgggc
     5341 gaatgcctct ctggcagatt cctcatcata aaaacccttg taaaatgctc cttgaaaacc
     5401 tttcactctt tccaggcatt cttcccagga atccacaact cctggctgcg ttcccctcca
     5461 gattacataa tatttatatt tcttcttcat tctatcgtat tatgaactta aaacattaaa
     5521 aaaacagctt attcatcact tcattaggta caaatatttc gttattaccg aaaaacaaac
     5581 gaaacccttt caccactttc aataaaaggc attcgaaagt aacataactg gttatcattt
     5641 catattgaat acaaaattag gacaatctca tcgaaaaata acgaaatgtt ccgagggaag
     5701 aattataccc acatattttt attaaaaaat tcgacagatc tgttatctcg atacaccccg
     5761 atggagtact tgacgatcga tggataatag gtggccaaac agggctgagc ttgtcgaagc
     5821 ccgtatcgtt gccataataa atcaatttcg tgtgctctgc tattttggtt tctttctctt
     5881 tctctttctc tttctctttc tctttctctt tctctttctc ttt
//
LOCUS       DAOORU010000149         5913 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_16344_length_5913_cov_5.724138, whole genome shotgun sequence.
ACCESSION   DAOORU010000149 DAOORU010000000
VERSION     DAOORU010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5913
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16344_length_5913_cov_5.724138"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(314..535)
                     /locus_tag="PLF20_08680"
     CDS             complement(314..535)
                     /locus_tag="PLF20_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81115.1"
                     /translation="MPRVKKDKLLQQIRVRDMAEVFTPLWICNAQNNLIDEAWFGRKN
                     VFNTEIVNPDGFHSWKTNPDKISFPENKP"
     gene            complement(1026..2408)
                     /gene="mnmE"
                     /locus_tag="PLF20_08685"
     CDS             complement(1026..2408)
                     /gene="mnmE"
                     /locus_tag="PLF20_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004320322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOX81116.1"
                     /translation="MESTICAPATPQGTGAIAIIRLSGPNTFRIIEKIFQPAKSSLKI
                     KDIKSHTVHLGNFHNEDEIIDEGLITVFRAPHSYTGQDVVEISCHGSRYIQNTLLSLL
                     MKHGAVMARPGEFTLRAFLNGKMDLSQAEAVADLVNAEARMAHQLAIRQMRGGFSSEI
                     QQMRQKLIDFTSLIELELDFPEEDVEFANREHLLELLGEIRQKLHNLIESFRYGNVIK
                     HGIPVAIIGKTNVGKSTLLNALLNEERAIVSEIPGTTRDSIEDTMVIEGITFRFIDTA
                     GLRQSKEPIESLGIERTYEKINQARIILYVFDVNETTIEEIKENLCDFQHHLTNPDKK
                     FILVANKIDLLIESPRQFADFVELDTVFISAKRGENIRQLTDLLVNTVKEQINTDVTI
                     ISNARHLEAIQKTDLALQKVESDIKQNLSPDLLSVDIRSALYYLGEITGTIANEDILE
                     NIFSKFCIGK"
     gene            complement(2747..4792)
                     /locus_tag="PLF20_08690"
     CDS             complement(2747..4792)
                     /locus_tag="PLF20_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HOX81117.1"
                     /translation="MINQQLLQPESIAIIGGSNDVTKPGGKVLKNLLDHHFKGKLYVV
                     NPKETKVQGLPCYNDARDLPPTDMAILAVPARLCPESVKILAETKNTKAFIILSAGFH
                     EESQEGARLEEEIVETVNKTGGCLIGPNCIGVMTPYHTSVFTTPIPELVPDGVDFISG
                     SGATAVFIMETAITNGLRFSSVFSVGNSAQIGVEEVLEYLDTHFDPLQSSKIKLLYIE
                     SIAKPDKLLKHASSLIEKGCKIAAIKAGTSAAGSRAASSHTGALSSSDTAVDALLRKA
                     GIVRCHSRQELATVGGIFTHPPLPGNRMAIITHAGGPAVMLTDALSNNGIEIPPLESP
                     ELLSKLYPGSSVANPIDFLATGTASQLAEIIDYCENRFDQIDAMAVIFGSPGLFEVYD
                     VYKVLDEKMRSCRKPIFPILTSIINVKKEIEYFISLSHVNFPDEVVFGNALTKVYNTP
                     PPAAPPTIIDINKELIRKVIDKTSSGYLPPQDVQKLLDASGIPRAKEIVAQNMDEALQ
                     AAQLIGYPLVMKVVGPIHKTDVGGVALNISDLEQVKQNFAKLMAIEDAVGVLFQPMLS
                     GTELFIGANWELKFGHLILCGIGGILIETLKDFSTALAPLSHDEAVSMIHNLKSYSLF
                     KGVRGKEGINEGIFTDILLRVSSLLQAAPEIREMDLNPLLGNSKSMTTVDARIRIEK"
     gene            5136..5717
                     /locus_tag="PLF20_08695"
     CDS             5136..5717
                     /locus_tag="PLF20_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81118.1"
                     /translation="MRIKLVGLVFLGIALLFGLGACKSTKSYEKAAKATVIDLPLSSK
                     EYRTDRDYFRAVQSGKSPDLATSKKIALHNAKSELAGNIQSTLKRVTEQFTNQRSIIN
                     GQEYANEFNELAREEVNRQLNVVKIIGDKLMREKDGSYIYFIAIEMSKENLLNQISDK
                     VSKDAKLQLEFDKQQFKKIFDEEMKKLEEESGY"
BASE COUNT         1722 a         1125 c         1175 g         1891 t
ORIGIN      
        1 aaattattaa aaatttaaat atttaataat cagtaacata acatttaaaa tcataccaaa
       61 aactctattt tttcctgttt tttcagggta aaatgctcaa gttgggataa gtgtttttat
      121 aagccgtatg tgcagcgtcg agccattcgc cggaagtatc caaattttcg ttgataatac
      181 ggagtttgcg gtcgagtatg ccgatgtgtt tctcgaccgg gatatattca ccggtatttg
      241 tgtcgtagcg gctagtaata taaggtgctt caccgcaggt gatttcgagg cgtgtatccc
      301 gtacatagtc tttctatggc ttattttccg ggaaactaat tttatcggga ttggttttcc
      361 acgaatggaa gccatcagga tttacaattt cggtgttaaa tacgttcttt cgtccgaacc
      421 atgcttcatc aatcaaatta ttttgagcat tgcaaatcca cagcggcgta aaaacttcgg
      481 ccatatctcg tacacgtatt tgctgaagaa gtttatcttt tttaacccgt ggcataatat
      541 aatcttacca tgtttgccag taatcagtct gtgtagaatt ggagaatcat attcgtatcc
      601 gctaccaaat tcgagataat tatcagtagt ccaaaagata tttttatgcg tagtatgatc
      661 gcgcaaaagt atttccagga catctggata tttctccata attcgttctc taatatatca
      721 attttattgt atattattcc ttccatttct atttttaaaa ttaattttaa ccatcagata
      781 aaaaggtagc acatattctt tgattttagg atatgaatag tttaagtcat tactatcaag
      841 ccaaccgtat aaaaggtttt ctacaaaatt aaaaaaattt gactcatcaa tgtaattctt
      901 ttaagcggtc attgcctttt taatcgtatt gagatccata gatttctata atttgttaag
      961 tttgattatt ttatacctca ggggtcttga agacaatgag gttaatagga tatttgtttg
     1021 atgaattatt taccaatgca aaacttactg aagatgtttt cgagtatgtc ttcgtttgct
     1081 atagtgcctg taatctcacc aagataatac agggctgatc ggatatctac cgatagaaga
     1141 tcgggggaga gattttgctt aatgtcggat tctacttttt ggagtgcaag gtcggttttt
     1201 tgaatagctt ccagatgtcg ggcattcgaa atgatggtaa catcggtatt gatttgttct
     1261 ttaacggtat tgacgagtaa atcggttaat tgtcggatgt tttcgcctcg tttggccgaa
     1321 atgaaaaccg tatcgagttc gacgaaatcg gcaaactgcc gtggcgattc gatgagaagg
     1381 tctattttat tggctaccag aataaatttt ttatcagggt tagttaaatg atgttggaaa
     1441 tcgcaaaggt tttccttgat ttcttcgata gtagtttcat ttacatcgaa aacataaagg
     1501 atgatacggg cttgattgat tttttcgtaa gttctttcga tgcccagcga ttcgatgggt
     1561 tctttgcttt gacgcaaccc ggccgtatcg ataaagcgga atgtgattcc ctctatgacc
     1621 atggtatcct caatgctatc gcgtgtagta ccgggaattt cggatactat ggctctttct
     1681 tcgttcaata aggcattcaa taaggtggat ttacctacat tggtttttcc gatgatagcg
     1741 actggaatcc catgtttaat tacattgcca tagcggaatg attcgatgag gttgtgaagt
     1801 ttttgtcgga tttcacccag tagttccaac aaatgctcgc gattggcaaa ttcaacgtct
     1861 tcttccggga aatcgagctc caattcgata agtgaagtga agtcgatgag tttttggcgc
     1921 atttgctgta tttccgacga aaatcctcct cgcatctgtc ggatagcgag ttgatgagcc
     1981 atacgtgctt ctgcatttac aaggtcggct acggcctcag cctgcgataa atccattttc
     2041 ccattcagaa aagcgcgaag ggtaaattcc ccgggtcttg ccattaccgc tccatgcttc
     2101 attaataaag acagcaaggt gttttgaata tacctcgaac catgacagga tatttcaact
     2161 acatcttgtc ctgtatacga atgtggagca cgaaatacgg ttataaggcc ttcgtctatg
     2221 atttcatctt cattgtgaaa atttcctaaa tgaaccgtgt ggctctttat atcctttatt
     2281 ttcagactcg atttagccgg ttgaaaaatt ttttcgatga tacgaaatgt attaggaccc
     2341 gagagacgaa taatggcaat agctcccgtg ccctgaggcg tagccggtgc acaaatggta
     2401 gattccatcg caaacatctc cgataaggta ttcagattgg attccatgat ttcttgtttt
     2461 ttttgattaa acttttccag atgctaaatt ggttcttttt tctgtaatgg tacaaattcc
     2521 tatttttctg aataagtcat tttatgcatt cttctctttt ggattaattt tatttgcaag
     2581 tcgacccaca tatgtcaatg ctcgaaatgc attgaccgta ctcacctttc ctccttttta
     2641 cctcttctcc ccgtcccctc gtctccgttc tcccttcctc ccttataaac ttttaacttt
     2701 caacttttaa ctcatctatc caccaaccac cgtttttctg gttcacttat ttttctatcc
     2761 tgatgcgggc atcaacggtt gtcatacttt tcgaatttcc aaggagaggg ttgaggtcca
     2821 tttcgcgtat ttcgggagca gcttgtaata atgacgaaac tcttaacaaa atatcggtaa
     2881 aaattccttc attgattcct tctttccccc ttactccctt gaaaaggcta taacttttca
     2941 ggttatggat catgctgacg gcttcgtcgt ggctcaaagg cgccaaggca gtcgaaaaat
     3001 ctttcagggt ttctattagt atgcctccaa ttccacaaag tataagatga ccaaatttga
     3061 gttcccagtt agcacctata aaaagctcgg tacctgagag cataggctga aagagcactc
     3121 ccacggcatc ttcaatcgcc atgagttttg caaagttttg tttgacctgt tcgaggtcgg
     3181 atatattcag cgcaacgccc cctacatcag ttttgtgaat agggcctaca actttcataa
     3241 ccaatgggta accaatcaat tgagctgctt gcaaagcctc atccatattt tgggcaacaa
     3301 tttctttggc tcgtggaatg ccactggcat ccaataattt ctgaacgtcc tgtggaggaa
     3361 gatatcccga tgaagtttta tcaataacct tgcgaatgag ttctttattg atatctatga
     3421 tagttggcgg tgcagccgga ggtggggtat tataaacttt agtcagtgca ttgccaaaga
     3481 ctacttcatc ggggaagtta acatgactca aggaaataaa gtattcgatt tcttttttca
     3541 cgttgatgat ggaggtaaga attgggaaga taggtttgcg acaactgcgc attttttcat
     3601 ccaatacctt gtaaacgtcg taaacttcaa aaaggcctgg actaccaaat atcacagcca
     3661 tagcatcgat ctgatcgaaa cggttttcgc agtagtcgat aatctcggca agctgactgg
     3721 ctgtgcctgt ggcaaggaaa tcgataggat tggctaccga tgaacctgga taaagtttcg
     3781 agagaagctc ggggctttcg aggggcggaa tttctatacc attgttggag agcgcatctg
     3841 tgagcataac cgccggacca ccggcatgtg tgatgatggc catacgattt cccggaagcg
     3901 gtggatgagt aaatattcct cccacggtgg ccaattcttg ccggctatgg catcttacga
     3961 ttccagcttt acgtaaaagt gcatcaacag ccgtatcgct actggaaaga gcacccgtat
     4021 gtgaagatgc tgcacgactt ccagcggcgc ttgtccctgc cttgattgcc gctattttgc
     4081 atcctttctc tatgagtgat gaagcgtgtt ttaatagctt atcaggctta gcaatgcttt
     4141 caatatataa tagtttgatt ttcgaactct gcaaaggatc gaaatgtgtg tccaaatatt
     4201 ccagaacctc ttcaacgcca atttgtgcac tgtttcctac cgaaaaaaca cttgaaaatc
     4261 gcaatccatt ggtaatagca gtttccataa tgaaaactgc ggtagctccg ctgcccgaga
     4321 taaaatctac tccatcgggt accagttcag gaataggtgt agtaaagacc gatgtatgat
     4381 agggtgtcat tacgcctata cagttgggcc caattaaaca gcctccagtt ttatttacgg
     4441 tttcaacaat ttcctcttcc agccttgcgc cttcctgact ctcttcgtga aaacccgctg
     4501 aaagaatgat gaatgccttg gtgtttttcg tttcagccag aatttttacc gattccggac
     4561 aaagacgtgc tggaacggcc aaaatagcca tatcggtggg gggaaggtcc cgggcatcat
     4621 tataacatgg caagccttgt accttagttt ccttcggatt cacgacatag agcttccctt
     4681 taaaatggtg atcgaggagg tttttcaata cctttccacc gggtttggta acatcgttgc
     4741 ttccaccaat aatagcaatg ctctcaggct gaagtaattg ttgattaatc ataaatttta
     4801 caattaagaa gatttctcga tgaataataa aatgattctt tggcggcgaa attatgaaat
     4861 cgcttcgata atcgaaaaaa gagattagga aaggaggctg tttcaggcat ttcgaaaagg
     4921 aagacgttcg gaaactgcaa cccattcctt tcgaaagatg agcacatcca aaacgataac
     4981 atttttagtt tttttcatga aaatagaatg ttctatttgt aaatcattgt atatcaattt
     5041 aattataaaa gtgaaaattt ttaaaaaaag tttttcaaaa agtgatgcga ttctcaaatc
     5101 ttgttgtata ttgggggcat taaaattata ccacaatgag aatcaaatta gtaggacttg
     5161 tatttcttgg aatcgcactt ttatttggat taggtgcatg taaatcgaca aaatcgtatg
     5221 aaaaagctgc caaggccaca gtaattgatc tgcctttgag cagtaaggaa tatcgaaccg
     5281 atcgagatta tttcagagcc gtacaatccg gcaaaagtcc tgatttagct acttctaaga
     5341 aaatagctct ccataatgcc aaatctgaac ttgcgggtaa tatccaatct actctaaaaa
     5401 gggtaacgga gcaatttacg aaccaacgaa gcattatcaa cggacaggaa tatgcaaatg
     5461 aatttaacga attggcacgt gaggaagtaa atcggcaact gaatgttgtt aaaattattg
     5521 gagataagtt gatgagagaa aaggatggca gctatattta ttttatagcc attgaaatga
     5581 gtaaagagaa tcttctgaac caaatcagtg ataaggtttc aaaagatgcc aaattacaac
     5641 tcgaattcga taagcaacag tttaagaaaa tttttgacga agaaatgaag aaattagaag
     5701 aagaaagcgg atactgaata aaataaaaaa taaacattat aaaaatttaa aaaataaaat
     5761 tagtacgaaa attaatttgg ttaatagaaa taattttttt atattagagt ctgtccatac
     5821 agtcatcaaa attttcagta aaatataaac ttcttatgaa agtttacctt tttgaagtta
     5881 ttcttggtcg attttataat ttttcaatct aaa
//
LOCUS       DAOORU010000150         5905 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_16372_length_5905_cov_6.045983, whole genome shotgun sequence.
ACCESSION   DAOORU010000150 DAOORU010000000
VERSION     DAOORU010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5905
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16372_length_5905_cov_6.045983"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1546
                     /locus_tag="PLF20_08700"
     CDS             <1..1546
                     /locus_tag="PLF20_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOX81119.1"
                     /translation="RTLREVYLPPFKACVDQGVATFMTSFNEIAGVPCTGSKYLLTDI
                     LRKEWGFEGFVVTDYTSINEMIPHGFAADEKHAGELALNAGVDMDMQGAIYYNYLKKS
                     LDEGKVKMADIDAAVKRILIMKFKLGLFDDPYLYCNKEREQQDIMRPEHLTFAREMAK
                     KSIVLLKNEQNLLPLSKNIGTIAVIGPVANNRRELIGSWSAAGDWTKAITLLEGIRQS
                     ISPETKILYAKGCSITDDSTQYIQEAINLAKKAEVVILAVGEAAWMTGEAASRSDLGL
                     PGIQQKLAEAIIATGKPVVTVLMNGRPLTISWLTEHTTAMLETWFGGTMAGPAIADVI
                     FGDYNPSGKLPVTFPRSTGQIPIFYNCKNTGRPFDASNKYTSKYLDIDNSPLFPFGYG
                     LSYTTFEYSNLRLNKNSLNMNDTLVITVSLKNTGKYGGTEIAQLYIHDKVGSVTRPVK
                     ELKGFYKVDLSPGEITEVRFKITKNDLAFCDINMNFTAEPGEFEVFVGSNSVDCLKTN
                     FWLQCN"
     gene            complement(1665..2390)
                     /locus_tag="PLF20_08705"
     CDS             complement(1665..2390)
                     /locus_tag="PLF20_08705"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOX81120.1"
                     /translation="MTVDIFIPCFIDQFYPDTGKNMVKIFERLGIEYHYNPKQTCCGQ
                     MSFNSGFWEDAKKIAIKFLKDFPNDRPIVCPSASCAGFIRNSYHHLFYNSAYHNEYKV
                     LKKNIWEFSDFMVSYLHVTDVGAVFDHIITYHDSCASLREYKLNGQPRLLLSNVRGLK
                     LVEMVQNDVCCGFGGTFAVKHEPISTAMAQQKIENALATGAEYIVSTDSSCLMHLQGY
                     IDKHEMPLKTIHLIDVLASGWKL"
     gene            complement(2444..3691)
                     /locus_tag="PLF20_08710"
     CDS             complement(2444..3691)
                     /locus_tag="PLF20_08710"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HOX81121.1"
                     /translation="MMNRLILTYRPIPSKVSIQLPYSKSISNRLLIIQALAGDTFEIA
                     HLSDADDTRVMAEAIRRYEEGFDVINVAEAGTAARFLTAYFALRAKPVILTGSGNLLR
                     RPISALVNTLRSMGAMIEYLDTPDRLPLHFKGGQLHGGNIQINADISSQFVSALLMIA
                     PMLPDPLNIHLINQEVSMPYVDLTIQCMRRCGAKARRLKNNMIEVDNSGYNFSGSIAM
                     EPDWSSAAPWFAISALQPSSEFLLENLDMNSLQADKLLIEAFKPMGVVTTSTHEGLLL
                     KWRGGVAKEMYFDFRNCPDIALSVIVTAAALNIDGVFANLSTLRFKETDRYQALKEQL
                     LRLGIDCAGKPHEGLIIRNRASFPPSATIFTYHDHRMALAFAPLACIMDYLIILEPQV
                     INKSYPSFWEHLGQIGIQLEYFD"
     gene            complement(3688..4764)
                     /locus_tag="PLF20_08715"
     CDS             complement(3688..4764)
                     /locus_tag="PLF20_08715"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HOX81122.1"
                     /translation="MKGYTEIKSFEHPILLTGSRKILQDSITNLPFDRIMVLCDRNTW
                     RDCFHYIAPALCKKSCHIHIIKEGEDSKNLSTLEGIWNILSAEGFRRDDALLNLGGGV
                     VCDMGGLAAATFHRGMQFIHVPTSLLAMVDAAIGGKNGVNFRGLKNQIGVFAFPYITL
                     IYPVFLKTLPNRQIQSGFVEMLKHALLNGDDLWNRLSAIEHEEVMHHSHYISEALKVK
                     VSYVDKDPFDHGIRQALNLGHTAGHVFEEWSLLNQSEPMLHGEAIALGMLVELGLSVK
                     LTGLDENFALLASNVFRKHIHLPVPTLQDIGQLIDLAGFDKKNKDHFIRFSLLQKVGK
                     PVVQVAVSRETLSHAFRNTILSLT"
     gene            complement(5146..5490)
                     /locus_tag="PLF20_08720"
     CDS             complement(5146..5490)
                     /locus_tag="PLF20_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HOX81123.1"
                     /translation="MNIEELREYCLSKNQAEECLPFDDNSLVFKVKDKIFAILSLTQP
                     FSIALKCDPSIAVSLRERFPSVLPGYHMNKRHWNTVLVDGSIPDELLENWIDLSYDLV
                     VSKLPVSLRNQL"
     gene            5663..>5905
                     /locus_tag="PLF20_08725"
     CDS             5663..>5905
                     /locus_tag="PLF20_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOX81124.1"
                     /translation="MNNEGMTLSQKIADLKQRIRVASEGGGPKAIESQKAKGKLTARE
                     RILALIDPKSFHEYDLFVANAGKDFDMDKKFLPGDGV"
BASE COUNT         1898 a         1265 c         1147 g         1595 t
ORIGIN      
        1 ccgtaccctg cgcgaagtct atctgcctcc cttcaaagcc tgcgttgacc aaggtgtggc
       61 aaccttcatg acttcattca acgaaattgc cggagttcct tgcactggca gcaaatatct
      121 actcaccgac atcctgcgaa aagaatgggg cttcgaagga ttcgtcgtaa ccgattatac
      181 ttccatcaat gaaatgatcc cacatggttt tgctgccgac gaaaaacatg ccggtgaact
      241 tgcccttaat gctggcgtag atatggacat gcaaggagcc atttattaca actacctgaa
      301 aaaatcactc gacgaaggaa aagtgaaaat ggcagacatc gatgcagccg taaaaagaat
      361 ccttatcatg aaattcaaat taggcctttt cgatgatccc tatctatatt gcaataaaga
      421 acgtgaacaa caagatatta tgaggcccga acatcttacc tttgcaagag aaatggcaaa
      481 aaaatccatt gttcttctaa agaatgaaca aaatctctta cccctgtcga agaacattgg
      541 cactatcgcc gtaataggcc ctgtagccaa taatcgtcgt gaactcattg ggtcgtggtc
      601 agcggcagga gactggacaa aagccattac gttgcttgaa ggaattcgtc agagcatttc
      661 acccgagacg aaaattctct atgccaaagg atgtagcatc acagacgatt ccacccagta
      721 catacaagaa gccatcaacc tggccaaaaa agccgaagta gtaattcttg cagtgggtga
      781 ggccgcatgg atgaccggtg aagcggcaag tagaagcgat ctgggattgc ctggtattca
      841 gcaaaagcta gctgaagcaa tcatagcaac gggtaagcct gtggttactg tactgatgaa
      901 tggccgtccc ctcaccattt catggctcac cgaacacacg actgctatgc tcgaaacatg
      961 gttcggtggt acaatggccg gtcccgctat agctgacgtc atcttcggcg attataatcc
     1021 ttcagggaaa ctccctgtaa ccttcccaag aagcacaggt caaattccta ttttctacaa
     1081 ctgcaaaaat accggccgcc ccttcgatgc cagcaacaag tacacttcaa agtatctcga
     1141 tatcgacaat tcacctctct ttccctttgg ctatggattg agttatacca ccttcgaata
     1201 ttccaatcta cggctaaata aaaattcttt gaatatgaac gatactctcg taataactgt
     1261 ttcactgaaa aataccggaa aatatggagg aacagaaatt gcccagcttt acattcacga
     1321 taaggtagga agtgtaaccc gacccgttaa agaattgaag ggattctaca aagtagatct
     1381 cagccctggt gagattactg aggtgagatt taaaatcaca aaaaacgatt tagccttttg
     1441 tgatatcaac atgaacttta ctgccgaacc cggagaattc gaagtctttg taggtagtaa
     1501 ttcagtagat tgtctaaaaa cgaacttctg gttgcaatgt aattaattgc atactacggg
     1561 cgtgttcggc caaaatctca aaaaatggtt ctggggaaga aaccgagcca atttttcctc
     1621 gacagaattt cattaaaact gatccatggg tgggtaaacc tgttctataa tttccaacca
     1681 ctggcaagaa catcgatcag atgaatcgtc ttgagaggca tttcgtgttt gtcgatatac
     1741 ccttgaagat gcatcaaaca ggaagaatcg gtagaaacga tatattcggc acccgtggct
     1801 agagcatttt caattttctg ctgagccatt gccgttgaaa tgggttcgtg ttttacggca
     1861 aaagtgcctc caaatccaca acaaacatcg ttttgaacca tttctaccaa cttaaggcca
     1921 cgaacattcg acagcaaaag acgtggctga ccattcagtt tgtattccct caaacttgca
     1981 caagaatcgt gataagtaat gatatgatcg aaaactgctc ccacatcggt tacatgcaga
     2041 taactcacca taaaatcgga aaactcccag atattcttct tcaaaacttt atattcgtta
     2101 tgataagctg aattataaaa cagatgatga tacgaatttc gaataaaacc tgcacatgaa
     2161 gccgaaggac atacaattgg cctatcgttc ggaaaatcct tcaaaaattt tatggcaatc
     2221 ttttttgcat cttcccagaa tccgctgttg aaagacattt gtccacaaca agtttgttta
     2281 ggattataat gatattctat cccaagcctt tcaaaaatct ttaccatatt cttgcccgtg
     2341 tcgggataga attgatcgat aaaacaaggg atgaaaatat cgacagtcat tgcaaagatt
     2401 aatagcgcaa aagtattaaa actatgttat ttttcgacaa atgtcaatca aaatattcga
     2461 gttgaatccc tatttgtccg agatgttccc agaaagatgg gtaggattta ttgataacct
     2521 gaggctccaa aataataaga taatccatga tgcatgcaag aggagcaaaa gcaagtgcca
     2581 tgcgatggtc atgataagtg aagatagtag ctgaaggagg gaatgaagca cgattgcgaa
     2641 ttattaatcc ttcatggggt ttacctgcac agtcaatgcc aagccttaaa agttgttctt
     2701 tcaaagcctg atatcgatct gtttccttga atctcaacgt gctcaagtta gcaaatacac
     2761 catcaatatt taaagctgca gcggttacta ttactgataa tgcaatatcc ggacaatttc
     2821 gaaaatcaaa atacatttct ttggctactc ctcctctcca cttcagtagc aatccctcgt
     2881 gcgtagaggt agtaacaacc cccattggtt taaatgcttc tataagtagc ttatcggcct
     2941 gaagagaatt catatcgaga ttttcgagta aaaactcaga ggatggctgg agtgcactta
     3001 tggcaaacca tggtgcagca gagctccagt cgggctccat agcaatgctt cctgaaaaat
     3061 tgtaacccga attatctacc tcaatcatgt tgtttttcag tcgtcttgct ttcgccccgc
     3121 atcttctcat acattgtatc gtcaaatcta cataaggcat cgagacttct tgatttatca
     3181 gatgtatatt caatggatcg ggaagcatag gcgcaatcat cagcaaagct gaaacgaact
     3241 gactgctgat atcggcgttt atctggatat tcccgccatg aagctggcca cctttaaaat
     3301 gcaaaggcag acgatcagga gtatccaaat attcgatcat ggctcccatc gaacgcaaag
     3361 tattcaccaa agctgaaata ggacgccgaa gcaagttgcc cgaaccggtt aaaatgacgg
     3421 gtttagccct caaggcaaaa taagctgtca aaaaacgagc cgcagtgcca gcctcagcca
     3481 cattaatcac atcaaaaccc tcttcatatc ttcgaatggc ttctgccatc acccgggtat
     3541 cgtcagcatc cgacaaatgc gctatctcga atgtatcccc ggcaagtgcc tgaattatca
     3601 acaacctgtt gctgatactt ttcgaataag gcaattgaat gctcaccttt gatggaatgg
     3661 gtcgataagt cagaattaac cgattcatca tgtcagagat aaaatagtat ttctgaaagc
     3721 atggctcaaa gtctcccgtg aaacagcaac ctgaactaca ggctttccta ctttctgcag
     3781 caaagaaaat cgaatgaagt gatctttgtt ttttttgtcg aaaccagcca aatcgataag
     3841 ttgtccaata tcttgcaaag tggggacggg taaatgaata tgttttctaa aaacattaga
     3901 ggcaagcaaa gcaaaattct catccaaacc agtcagtttt accgacaacc cgagttcgac
     3961 gagcattccc aaagctatag cctcgccatg gagcatgggt tcggattgat tcaacaacga
     4021 ccactcctcg aaaacatggc cggcggtatg ccctaaattg agagcttgcc ttattccatg
     4081 atcgaaagga tctttgtcta cgtaagagac ttttaccttc aaagcttcag aaatgtaatg
     4141 cgaatgatgc ataacctctt catgttcgat tgcgcttaaa cgattccaca aatcatctcc
     4201 atttagcaag gcatgtttca gcatctcaac gaaccccgat tgtatctgcc tattaggtaa
     4261 ggttttcaaa aatacaggat aaatcaatgt tatataagga aatgcaaaaa cgcctatctg
     4321 attcttcaga cccctaaaat tgactccatt ttttccgcca atggccgcat ccaccatggc
     4381 caataaagaa gtaggcacat gaataaattg cattccccga tgaaatgtgg cagcagccaa
     4441 cccccccatg tcacacacca caccacctcc taaattcagc aaggcatcgt cgcgtctaaa
     4501 tccttctgcc gataaaatat tccaaatgcc ttccaatgtg gaaaggtttt tcgagtcctc
     4561 cccttctttg ataatatgaa tatgacaaga ttttttgcaa agtgcaggtg caatgtaatg
     4621 gaaacaatct ctccatgtat tacggtcgca taaaaccatg atgcgatcga atggcagatt
     4681 ggtaattgaa tcttgtaaga ttttcctact acccgtaagc aggatgggat gttcaaagga
     4741 ttttatttca gtataaccct tcataagatg atggcagttt aaaaccttgt ttcattatat
     4801 aaatcaaaat caataacata tttattttct actttttgca aaattttgta attaatttaa
     4861 tttcagcaag ataaaaagga aaatatattc acttatttaa atttatttat ttttacaaaa
     4921 atttttctgt caaatatcat gaagccaaag ataagaagga ttcgtttttt caagataatg
     4981 gggaaagtcg ttggcctggc aatgggatat ccatattaca agcaaatagg ctgcaatgct
     5041 ggtggatgcc gaatcacctc gaattcttat ctaagtgtat tatgggggat gtttatcggg
     5101 gtatttgatt gtcatcatat tgcttatacc tcggaaatac agccctcaaa gctgatttcg
     5161 taaagataca ggcaatttcg atacgaccaa atcatagctg aggtctatcc aattctctaa
     5221 tagctcatcc ggaatgcttc catcgacgag aacagtattc caatgccgtt tattcatgtg
     5281 atatcctggc aagacggaag ggaatctttc acgcaatgaa acagcaatcg aaggatcaca
     5341 tttaagagct atggagaaag gttgggtgag gctcaagata gcaaaaatct tatctttaac
     5401 tttaaaaacc aaagagttat catcgaaagg caggcattct tcggcttgat tcttcgaaag
     5461 gcaatactca cgcaattctt cgatattcat gattaacatt tattaacaaa agtaagaaaa
     5521 tcatataaca tacacataac cagtcgaaac agtaaaaatt attttcgacc aaaagagagt
     5581 aaataaaaat agcgtttgaa gaaaggtggt tttcctataa atttgcttcc ctttggaaaa
     5641 atcttactaa aacctcacac aattgaacaa cgaaggaatg acattaagcc aaaagattgc
     5701 cgacctcaag caaaggataa gggtagcatc ggagggaggc ggccccaagg ctatagaaag
     5761 tcagaaagcg aagggtaaat tgactgcacg ggagcgaata ttagctctaa tagatccaaa
     5821 gtcatttcac gaatatgatc tttttgtagc caatgccggg aaagattttg acatggacaa
     5881 gaaatttctt cccggcgatg gagta
//
LOCUS       DAOORU010000151         5807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_16707_length_5807_cov_5.180111, whole genome shotgun sequence.
ACCESSION   DAOORU010000151 DAOORU010000000
VERSION     DAOORU010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16707_length_5807_cov_5.180111"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1606)
                     /locus_tag="PLF20_08730"
     CDS             complement(<1..1606)
                     /locus_tag="PLF20_08730"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HOX81125.1"
                     /translation="MKNKQGTGQKIYTFEEALQASTEYFKGDAMAANVWVSKYALKDS
                     ENKIYELTPDDMHWRIANEIARIEARYPHPMNKEEIYELLKQFRYIIPQGSPMAGIGN
                     NIQLGSLSNCFVIGQEKPADSYGGIMRIDQEQVQLMKRRGGVGHDLSHIRPKGSPVKN
                     TALSSTGIVPFMERYSNSTREVAQDGRRGALMLTISVKHPDVEDFIDAKLEAGKVTGA
                     NISVKLTDEFMEAVKNNREYVHQFPIDAEKPSFTKTVYARQLWDKIIHNAWKSAEPGI
                     LFWDTIIRESIPDCYADYGFRTISTNPCGEIPLCPYDSCRLMAINLYSYVENPFTPQA
                     FFNAELFTVHVRKAQRIMDDIIDLELEKIDRILEKITQDPEDEETKRIERLLWENIRH
                     KAVLGRRTGLGITAEGDMLAALGYRYGSDEAINFCVAVQRTLALEAYRSSVEMAVERG
                     PFEIYDSQREENNPFILRLKEADEELYHNMVKHGRRNVSLLTVAPTGSVSILTQTSSG
                     IEPVFAVRYKRRRKINPSGFPVSKISY"
     gene            2410..2586
                     /locus_tag="PLF20_08735"
     CDS             2410..2586
                     /locus_tag="PLF20_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81126.1"
                     /translation="MIRTIPRKPTKPHEMTVKMLCTLTSELLIRFLSGCRGFDDGVIA
                     LPPLNNHSYHPLEI"
     gene            2898..3533
                     /locus_tag="PLF20_08740"
     CDS             2898..3533
                     /locus_tag="PLF20_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOX81127.1"
                     /translation="MITVKSFVFNDFQTNTYILSNEQHQCMIIDCACQNATEWQQLKG
                     WLNENQLTPLHALLTHCHVDHVLGCHYLLDEYGIGYQTHPASKIFIELASEFSSVLGL
                     KLEKFPPPQSYIYEGDCIQMEGLTLKVIYTPGHADGSVCYHSENEKIMFSGDVLFKDS
                     IGRSDLPTGNYEKLRKSIIEKLFVLPDDTVVYPGHGPFTTIGHERKNNPFV"
     gene            complement(3537..4544)
                     /locus_tag="PLF20_08745"
     CDS             complement(3537..4544)
                     /locus_tag="PLF20_08745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOX81128.1"
                     /translation="MLHVHKILVLRFSSIGDIVLTTPVIRCMHRQLENAEIHYLTRTV
                     YMPLLEHNPYIHRLMGFDHSPQELLPRLKAEHYDFIVDLHRNFRTVALRLRLLRPSAS
                     FPKLNIRKWLMVNFHINTLPKIHIVDRYFMAIKRLGVLNDGKGLELFISQKDEVALSE
                     LPANFWNGYGVIAIGAKHYTKQIPVELATEIVSLAPLPFLLVGGIEDYDKGEQINHKI
                     PLKSYNICGKYSLPQTASIVKNAKIVITSDTSIMHIAAAFHKPIISIWGNTLPEFGMY
                     PYMPGNEEKSILIENKELKCRPCSKLGYTECPKEHFKCMRQHNPQQVIGAIHKLLADS
                     R"
     gene            4589..5383
                     /locus_tag="PLF20_08750"
     CDS             4589..5383
                     /locus_tag="PLF20_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008024783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOX81129.1"
                     /translation="MKIIDTHTHLYLSEFEEDLSKVVEEAIRVGVSKFLLPAIDSTTH
                     KHLLSLTNSYPEHFYPMMGLHPTSVKENYMDELAIVHHYLTNHSFVAIGEVGIDLYWD
                     MTFEKQQVKAFKIQAQWAREMGLPIVVHSRNSTEVILQVLEEIGYKGLSGVFHCFNGT
                     LEQALEIASKGFYLGIGGVITYKNSSLSSIVARVPRDFVVVETDAPYLPPVPHRGKRN
                     QPAWIKYTVNKLAEIWQISPEEAAGITTANAIRLFQKNCDLSKLKL"
     gene            5388..>5807
                     /locus_tag="PLF20_08755"
     CDS             5388..>5807
                     /locus_tag="PLF20_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOX81130.1"
                     /translation="MNENSSVLIIYTGGTIGMMHNPITGALEPLDFNHLYKHLPVLEN
                     LKCRIDTYSFDPVIDSSDMNPDFWIELAEVIEKNYESYDGFVVLHGTDTMAYSASVLS
                     FMLENLNKPVVFTGSQLPIGMIRSDGRENFITAVEIAT"
BASE COUNT         1635 a         1202 c         1133 g         1837 t
ORIGIN      
        1 tataggagat tttactgaca gggaatccag aggggtttat cttgcggcgt cgtttgtatc
       61 tgacggcaaa aacaggttcg atccccgatg aagtctgcgt aagtatgctg acgctccccg
      121 taggggctac tgtgagcaat gagacatttc ggcgaccatg tttcaccatg ttgtggtaaa
      181 gttcctcatc ggcttctttc aaacgaagga taaaaggatt gttttcttct cgctgcgagt
      241 cgtatatttc aaatggccct ctttctacgg ccatttctac tgaagagcga taagcttcga
      301 gagctaatgt tctttgcaca gccacgcaaa aattaatggc ttcatcactt ccataacgat
      361 aacccagtgc ggccagcata tcaccttccg cggttatacc taaacccgtc cttcgtccca
      421 atacggcttt atgacgaatg ttttcccata gcaaacgttc tatgcgtttg gtttcttcat
      481 cttcgggatc ttgagtaatc ttttcgagta tcctgtcgat tttctctaat tccaaatcga
      541 tgatatcgtc catgatgcgt tgtgctttcc ttacatgcac agtaaaaagc tcagcattga
      601 agaaggcctg gggggtaaag ggattttcga cataagaata aaggttgatg gccatcagac
      661 gacagctatc gtaagggcag agtggaattt ctccacaggg attggtgctt atcgtgcgaa
      721 aaccataatc ggcataacaa tcaggtatgg attcccttat aatggtatcc cagaacaata
      781 tgccgggttc ggctgatttc caagcattat gaatgatctt atcccatagc tgacgtgcat
      841 aaacagtctt tgtaaatgat ggtttttcgg catcgatagg aaattggtga acgtattccc
      901 gattgttttt caccgcctcc ataaattcat cggtcaattt taccgagata ttagcccccg
      961 ttacttttcc tgcctccagt ttggcatcga tgaaatcttc aacatcggga tgtttcacag
     1021 aaatagtcag cattaaggct cctctccgac cgtcttgagc aacctcacgc gtagaattgg
     1081 aataacgttc catgaaaggg actatccctg tggaagaaag ggcagtattt ttaactggac
     1141 tgcctttcgg acgaatatgc gacaaatcgt gcccaactcc tccccttcgc ttcatcaact
     1201 gcacctgttc ctgatcgatt cgcatgatgc ctccataaga atcggctggc ttttcctgcc
     1261 caatcacaaa acaattgctg agactgccaa gctgaatgtt gttaccaatg ccggccatag
     1321 gactcccctg aggaatgatg tatcggaatt gtttcagcaa ttcgtatatc tcttccttat
     1381 tcataggatg aggatacctt gcttcgatgc gtgcaatttc gttcgctatc cgccaatgca
     1441 tgtcatcggg cgtcaactca taaattttgt tctcgctgtc cttgagggca tatttgctaa
     1501 cccatacatt ggccgccata gcatctcctt tgaaatattc cgttgaggct tgaagcgctt
     1561 cctcaaaggt gtatatcttt tgccccgttc cctgtttgtt cttcatcgtt ccttcttcct
     1621 tttcttccat tccttcaaat aaatctctca tcatggcatt ggcttttaat ccagctttcc
     1681 gtcttcgtaa aacctcttca tacaattgat cttcctcttt atcaggaata tcccgagggc
     1741 tttctggatt tattttgttt tcctgtatca taattttatc tgctcctggt ttcattaaat
     1801 tcggcttaat caagcggttt taaactttta aacaaaagcg gaaaccgtgc cccaaaataa
     1861 ttcaaaataa tcttaatttt cactcattga ttacactttt tatccccttt ttatccacaa
     1921 tcttaaaatc tattgataat ctattgaatg ttagaagatt ctataaacac ttgataatca
     1981 atcaaaattc tgaactactt cagcagttca tcctctcatc atcgaatggt tagaaacaaa
     2041 tgaattgttt ttcgagtttc taattagatt ttgatggaat gatacttatt atccctcgag
     2101 gtattcgaaa aatttataga attgattatt acctgtggtt ttcgaaaagc attcttagat
     2161 cggcgattaa aagaacaatg gcaaaatttt aactgcctga tcattaataa aaatggcaaa
     2221 tttcgagcca aaatatcgct catgcgagaa agccatcgca agaattatag cataatcgat
     2281 atcgatgaat cgaattttat aataatcaat tgtctcccgc aggtcaatgg gaggatacaa
     2341 tacatcaccg cctgattctt gccttgagct tttttctgta tgctctctaa aaatatgaac
     2401 attctcgaaa tgataaggac catcccacgt aagcccacga aaccacacga aatgaccgta
     2461 aaaatgttat gcacattaac atcagaactc ctgattcggt ttctatcagg ttgccgtggt
     2521 tttgatgatg gcgttatagc attacctcct ctgaataatc actcctatca ccctcttgag
     2581 atttagtccc atcatctgca accgctcatt cttttttaat tttttcccag acattgaaat
     2641 ctaaaaagtt ttgcctatta gcgagaaaag aattgcacat agaagcgcaa agtgcgctaa
     2701 ggatttgaag tggttttgtt agtgtttata agataggact tttgtaaaat atcgataggc
     2761 aattatagga taaggagttc tgaaatttta gtttcataat aaaatgcccc ggaaatagcg
     2821 taggatgttt ttctctctat ccaaacttta ttctgttgga tgaattcttt tacttttgct
     2881 tttataactg tttacagatg attacagtaa agtcctttgt ttttaatgat tttcaaacga
     2941 atacgtacat tttgagtaat gaacaacatc aatgtatgat tatagactgc gcctgccaga
     3001 atgctactga atggcaacag ctcaagggat ggctgaatga aaatcaacta acgcctttgc
     3061 atgctcttct tacccactgt catgtcgatc atgttttagg atgccattac ctgcttgatg
     3121 aatatggaat aggatatcaa acacatcctg cttccaaaat ttttatcgaa ctggcttcag
     3181 aattttcatc cgtgttgggg ctgaagctcg agaagtttcc ccctcctcaa tcgtatattt
     3241 acgaaggaga ttgtatacaa atggaaggat taaccctaaa ggtgatttat acaccgggcc
     3301 atgctgatgg tagtgtttgt tatcattcgg aaaacgaaaa aattatgttt tcaggtgatg
     3361 ttttatttaa agatagtatc ggcaggtctg atttaccaac aggcaattat gaaaaactca
     3421 gaaaaagtat aatcgaaaaa ctctttgttt tgcccgacga tactgtagtt taccctggcc
     3481 atggcccttt taccactatt ggtcatgagc gtaaaaataa tccttttgtt tgatgttcat
     3541 cgggaatcgg ccaataactt atgaatagct cctatgactt gctgagggtt atgttgcctc
     3601 atgcacttaa aatgttcttt cgggcactca gtatagccta atttactgca tggccggcat
     3661 tttaactcct tattttctat caaaatagat ttttcctcgt ttccaggcat gtagggatac
     3721 attccaaatt caggcagtgt atttccccag atgctaatga tgggtttgtg aaaagctgct
     3781 gcaatatgca taatgcttgt atcagaggta atgacgattt tggcattttt aacaatagat
     3841 gcagtttgcg gcagagaata ttttccacag atattgtatg atttcaatgg aattttgtgg
     3901 ttgatttgct cacctttgtc ataatcttcg attccaccga caagcaaaaa cggtaaaggc
     3961 gccaggctaa cgatttctgt ggccagttcg acaggaattt gtttggtata atgctttgct
     4021 ccgatagcaa tcactccata gccattccaa aaattagcgg gtaattccga aagagctact
     4081 tcatctttct gtgaaatgaa taattcgagt cctttcccat cattcaaaac tcccaaacgt
     4141 ttaattgcca tgaagtatct atccacaata tgaatctttg gcagtgtatt gatatgaaaa
     4201 ttcaccatca gccattttcg aatgtttaat ttaggaaagc ttgccgatgg cctcagaaga
     4261 cgaagcctaa gagcgacagt gcgaaaattg cggtgaagat cgacgatgaa atcgtaatgt
     4321 tcggctttaa gccgaggcaa taattcctga ggactatgat cgaagcccat taaccgatga
     4381 atgtaaggat tgtgttcaag caaaggcatg tatactgttc gggtcaaata gtgtatttct
     4441 gcattttcga gttgacgatg catgcaacgg ataactggcg tggtgagaac gatatcgcca
     4501 attgaactga agcgaagcac aagaatttta tgcacatgta gcatttgacg caaatttaga
     4561 aaatttgatt ttctggtaat tttgtgccat gaaaattata gatacccata cacatcttta
     4621 tctgtccgaa tttgaagagg atttgtcgaa agttgtcgag gaggccattc gagtgggagt
     4681 aagcaaattt ttactcccag ccatcgacag cactacccat aagcatcttc tatctcttac
     4741 gaattcttat cccgagcatt tttatcctat gatgggtttg catccaacct cggtgaagga
     4801 aaattacatg gatgaacttg ccattgttca tcattattta acaaatcatt catttgtggc
     4861 cattggagag gtaggaatcg atttatactg ggacatgacc ttcgagaagc agcaagtgaa
     4921 agctttcaag attcaagcgc aatgggcaag agagatgggt ttgcctatcg ttgttcattc
     4981 acgaaattcg acagaagtga tactgcaggt gctcgaagag attggttaca aaggccttag
     5041 tggagttttc cattgtttta acggtactct tgaacaagct cttgaaatcg cttcaaaagg
     5101 tttttatctg ggtattggag gtgtcattac ctataagaat tcatccctct cctctatcgt
     5161 tgcccgagtg ccacgtgatt tcgttgttgt ggagaccgat gcaccatatt tacctcctgt
     5221 acctcaccgt ggtaaacgca atcaacctgc atggataaag tacactgtga acaaacttgc
     5281 cgaaatctgg caaatctcgc ccgaagaagc tgccggtatt accactgcaa atgcaatccg
     5341 gcttttccaa aaaaattgtg atttgtcgaa attaaaatta taaccttatg aacgaaaata
     5401 gttccgtgtt gatcatttat acaggtggaa ccattggtat gatgcataat cctattacag
     5461 gagctctcga gcctctcgat ttcaaccatt tatataagca tttgcctgta ttagagaacc
     5521 tcaaatgtag aattgatacc tattctttcg atccagtcat tgattcatcc gacatgaatc
     5581 ccgatttctg gatcgaactg gccgaagtca tcgaaaagaa ttatgaaagt tatgatggat
     5641 tcgtcgtctt acatggtacg gatactatgg cctactctgc ctcagttttg agcttcatgc
     5701 ttgaaaattt gaataaaccc gtagtattta ctggatctca gctgcccatt ggcatgatac
     5761 gttccgacgg gcgtgaaaat ttcattacgg ctgtcgaaat tgctaca
//
LOCUS       DAOORU010000152         5677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_17188_length_5677_cov_6.239417, whole genome shotgun sequence.
ACCESSION   DAOORU010000152 DAOORU010000000
VERSION     DAOORU010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5677
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17188_length_5677_cov_6.239417"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            150..638
                     /locus_tag="PLF20_08760"
     CDS             150..638
                     /locus_tag="PLF20_08760"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81131.1"
                     /translation="MCSIRHNNPEESLKAAELTVAFKNKGIVGFDLAGAEDGFPAKHF
                     KEAFSLIINNNINATVHAGEAYSPESIHQALHMVSANRIGHGTRLRESGDLMNYMNDH
                     RIPIEICITSNVQTKAVDSLQNHPIPFYYDYGLRVTLNTDNRLILNTTLTNEYMIAIK
                     NF"
     gene            1173..3239
                     /locus_tag="PLF20_08765"
     CDS             1173..3239
                     /locus_tag="PLF20_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81132.1"
                     /translation="MKNNHFKYSTALFLGIITQLTQISWLRSIFALFDGNEILSGICL
                     AIWMLFTGLGTKINGQQFFGKHSIFPSIAFVMQGFFPLIMDVLAIAFRNHIFIAGVMP
                     PIHWVAAFIFVWLAPFCFLSGMLYAWLVLQADGPKIYGMESIGGMLAGIFSIVLLLLF
                     PNNQISFYLIIALAFINTTLWLNIQYRFKFLFTLVLFILIIVPLIKVNFPEKANQKLF
                     EGQHLLSTLETPIGRLHLTEYYGDTNIYVNGQPYCSSYRGPEPTENLHFTMLQKPYAK
                     SLLILGEIVPRLLEETQSWNFDQIVAIEPNPFVWPWIEEFIKSPAIPNIRLEKGDAIH
                     QIKTLHETWDVIIINNSDPVNLSSNRWLCVEFFCLLKEKLNHNGIICCRLPSAVNYPE
                     SQMLRVYSCLYQTLSKYFSQVLPVIGTQTWFLASDSPLNPNWHVLMKNPPVASNYVNP
                     NYFDPQELISKSNDLLSRLDRDAPLNTAFRPSAFAFQSFYWLRLNSAKLLWIFPAIIV
                     LIIILLWRQPRKGIIVFLGGFTASTGQILILLLTQIILGNLYILTGLIFGLFMGGLAL
                     GSLSKSFSNAKPSFVLLFMGLTLLVPSAILSRIGPWRFPEFLSYFIIFADVIGLSYLM
                     GLLYRISLATSQTLAAKFYAADLAGATLGMLIPTLFLIPFLGIPATASIITLICIIAS
                     IMNYKL"
     gene            4246..5364
                     /gene="amrS"
                     /locus_tag="PLF20_08770"
     CDS             4246..5364
                     /gene="amrS"
                     /locus_tag="PLF20_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOX81133.1"
                     /translation="MTRRDFLQNSLCGACGMALAWKGLDVFSMNPASLGDEGHEAMFY
                     MNTPRGIKCKLCPHACTIELGKRGDCRVRENRKGRLITLAWSNPCSIHVDPIEKKPLY
                     HFLPGSQSFSLAIAGCNFTCLNCQNWEISQTSPDKTRNYSLSPEEAVKQAKAQKCQSI
                     AYTYSEPVSFYEYTLETSRLALKAGIKNLLISNGYINPAPLDELCNYIDAANIDLKAF
                     DDDTYTMLANGTLQPVLDTLVKLKQKGVWLEITNLIVPKWTDDEKMIDKMCRWLSSNG
                     FKDTPLHFSRFYPQYKLNRLPPTPVSILERAKKIAENYGLQYVYIGNVPGAEQGTTFC
                     PKCKTSLVERIGFKVKKMVIENGRCPRCGATIPGKWSA"
BASE COUNT         1764 a         1082 c          979 g         1852 t
ORIGIN      
        1 gtgcagaagg aaacatcagg tatcttgaga ttcgatttgc tccggtattg gatacacaaa
       61 agggtttgtc gttgtagatg tcgttcaggc agtattgaat ggaaaaaaac gagccgaatg
      121 taatttcaat atgattatta ggaatcatta tttgttctat tcgtcataat aatccagaag
      181 aatcgctcaa ggctgcagaa cttacagtag ctttcaaaaa caaaggaatt gtaggtttcg
      241 accttgcagg agccgaagat ggtttccctg ccaaacattt caaagaggct ttttcgctga
      301 taatcaataa taatatcaat gcgacggtcc atgccggtga agcatacagt cccgaatcga
      361 tccatcaggc tcttcacatg gttagcgcga acagaattgg acatggtact cgactgcgcg
      421 aaagtggcga cctgatgaac tacatgaacg accaccgcat tcctatcgaa atatgtatca
      481 catcgaacgt tcaaaccaaa gccgtcgaca gtttacaaaa ccaccccata cccttttatt
      541 atgattatgg cttgagagtt acactgaata ccgataatcg tttaatttta aatactactc
      601 ttacaaacga atatatgatt gccatcaaaa atttttgatt tactgataaa gatcttatcg
      661 tattaataat caatggattc aaatcagcct tcctcaacca ttccgaaaaa gttcaactca
      721 ttcatcgagt attggatgaa atggctgatt taggtatgcc tgtaaaacgc gaatatctat
      781 aaattaaccg aaaaataaat tataagttca taaaacttga ggtggaattt atttgccttc
      841 gttaattgtc attccattga ctgataacga cttttacgaa agaattgtaa tgcctttcac
      901 cagaatttta tcagaaaata ttcgtcattg aaattatatt actctgtttc gaaaatttac
      961 ttgaagatta aattttacct tagcagagat ttcatcttca agaaaacact aaattcagaa
     1021 gaggatatcc caaaatatat tgattagtaa gtatatataa aaaggcttac catcatattt
     1081 ttattatgga ataaaaaaat gtattttcgt tggaaaattt ataggttaga attaatgcta
     1141 aaggaattat tttgtaaaaa atttctaagc gaatgaaaaa caatcatttc aaatactcta
     1201 cggcattatt tcttggaata ataactcagc ttacgcagat aagttggtta aggagcattt
     1261 ttgcgctttt tgacgggaat gagattcttt ctggaatttg ccttgcaatc tggatgttgt
     1321 ttacgggttt gggaacaaaa ataaatgggc aacaattttt tggcaaacat agtatatttc
     1381 catccattgc cttcgttatg cagggtttct ttcccctaat tatggatgtt ttggcaattg
     1441 catttcgtaa tcatattttt attgcaggcg tcatgcctcc aattcattgg gtagctgcat
     1501 ttattttcgt ctggctcgct cctttttgct tcttgtcggg aatgctatat gcatggcttg
     1561 tgctgcaagc cgatggtccg aaaatttacg ggatggaatc cattggtggc atgctggcag
     1621 gtattttttc tattgtcttg ctgcttttat tcccgaataa tcagataagt ttctacctga
     1681 tcattgcact tgctttcatc aatactaccc tttggctaaa tattcaatac cgtttcaaat
     1741 tcctgtttac attggttctg tttatactca ttattgtgcc tttaataaag gtaaattttc
     1801 cagaaaaagc caatcaaaaa cttttcgaag gccagcattt gctttcgacg cttgagactc
     1861 ccataggcag gcttcatctt accgagtact acggagatac gaatatctat gtgaatgggc
     1921 aaccctattg tagttcttat cgaggccccg aaccgactga aaatctccat tttaccatgc
     1981 tacagaaacc ttatgcaaaa tctctgttga ttttagggga aatcgttcca cggctgctcg
     2041 aggaaaccca atcatggaat ttcgaccaga tagttgccat cgagcccaat ccctttgtat
     2101 ggccatggat cgaagaattc attaaaagcc cggctattcc gaatattcga cttgaaaaag
     2161 gagatgccat tcatcagata aaaacgctgc acgaaacatg ggatgtaatt atcatcaaca
     2221 acagcgaccc cgtaaattta tcttccaata gatggttatg tgttgaattt ttctgcttat
     2281 tgaaagaaaa attgaatcat aatgggataa tatgttgcag gcttccttcg gccgtaaatt
     2341 atcccgaaag ccaaatgctt cgagtatatt catgccttta ccagacatta tcgaaatatt
     2401 ttagccaagt tcttcctgtt atcgggactc aaacttggtt tctcgcttct gattcgccgc
     2461 tcaatcctaa ctggcatgtt ctgatgaaaa accctccagt agcttccaac tatgtgaatc
     2521 ctaactattt cgatcctcag gaactcatta gcaaaagcaa tgacctccta tcgcgactcg
     2581 atcgggatgc accactcaat actgcttttc gtccttcggc ttttgctttt caatcatttt
     2641 actggctcag gttgaattca gctaagcttt tatggatttt ccccgcaatt atcgtgctga
     2701 tcataattct tctgtggcgc caaccccgca aaggaataat agtattttta ggtggattta
     2761 ctgcctcaac aggccaaatt ttaatccttt tgcttacaca aatcattttg ggcaaccttt
     2821 atattttaac cggtctaatt tttggacttt tcatgggggg cttagcctta ggctctcttt
     2881 cgaaatcatt ttcaaatgca aaaccttctt ttgttctttt atttatggga ttgaccttgt
     2941 tggtcccctc tgctattctt agccgtattg gcccatggag gtttccagaa ttcctttctt
     3001 acttcatcat ttttgccgac gtgattgggc tttcctatct catgggattg ttgtatagaa
     3061 ttagtctcgc tacttctcaa actctggcag ccaaattcta tgccgccgat cttgcgggtg
     3121 ctacacttgg catgttgatt ccaacactct tcctaatccc attcttgggt atcccagcca
     3181 ctgcatccat tattacactc atctgcatta ttgcttctat tatgaattat aaactataaa
     3241 aaattatcga cttaaatcga aattaaaatg atattttcga taaagttaaa aagtatcatc
     3301 atctgataaa aatagtttct ggaaacttcc acaaatcctt tgaaaaattt atttgttttt
     3361 ccccactaat taatcaaaca aattgaaaag tattcaaata taaccaattg cactaaaaat
     3421 tctaaaaatc caattttcac ttgaaacgaa taaaattcag ttttatctgg atttaagttt
     3481 gtatctaatc attatttgtt tttgtgtata ttttttgccg tttattgcat ttataaaggg
     3541 cttaaaattt ccaaggatat ttttatctta ttattttaat gattattcca tttttttaaa
     3601 tcacgaaatc cttcagaata cgaaaagaga aagactcgag cctgacttaa ccctttctta
     3661 catatttcgt aacgtattat gaatgaattt atttaatcct gcaatagaag aatgaaaaaa
     3721 tattatttac tctttacatt ttaactattt attatgaggt taggaggatt taataagata
     3781 gccgtatttc aagaatagaa tattggaaaa gaggcaggta ataatcataa cgaaacaaaa
     3841 aatcaggcaa gggattcatt gtataaaaaa cttttccaaa atatcaggcc aattgttcat
     3901 acccaaaaga aaccatgata ttttcatcat taaaaacagg gttcttatta taaaggtttg
     3961 tactcatgaa gaaactcagt acgatgataa ttacaaaaag attcaacccg aacaattatt
     4021 attcaggcgg tgggaaatct accctgcaac ttttctcaac ggtaaatatt ttgttctcga
     4081 taatacgcca tctcaacaca aagggctttt ttgataaaga gactccattc attttttatc
     4141 catttcatga aactatgctt aaaatattta ataaatcttc tgttgaattt tctaaaatga
     4201 agtaactttg tgaaatggaa ttatggaagt aaaggaaaca atagcatgac aagaagggat
     4261 tttttacaga attcgttatg tggtgcttgt gggatggctc tggcatggaa agggttggat
     4321 gtctttagca tgaatccagc tagtttgggt gatgaggggc atgaagccat gttttatatg
     4381 aacacaccga gaggaatcaa gtgtaaactt tgccctcatg catgtacgat agagcttggt
     4441 aaacgaggtg attgcagggt aagagaaaat cgaaagggtc gtttaattac tcttgcttgg
     4501 tcgaatccat gttctattca tgtggatcct atcgaaaaga agccacttta tcatttttta
     4561 cctggttctc aatcattctc cttggcaata gctggttgca actttacctg tttgaactgc
     4621 cagaattggg aaatttcgca aaccagcccc gataaaacac gtaattattc actttcaccc
     4681 gaagaagctg tgaaacaagc caaggcacaa aaatgccaaa gcattgccta tacttattct
     4741 gagcccgtta gtttttatga atatacactc gaaacttctc gcctagcgct aaaagccggt
     4801 ataaaaaatc tactcatctc gaacggatat attaatcctg ctcctctcga tgaactttgc
     4861 aattatatag atgcagctaa catcgatctg aaagctttcg atgacgacac ttatactatg
     4921 cttgctaatg gcacattgca gcctgtgctc gataccttgg tcaaactcaa acaaaaggga
     4981 gtctggctcg agataaccaa tctgattgtt cctaaatgga ccgatgatga aaaaatgatc
     5041 gataagatgt gtcgttggct gtcttccaat ggatttaaag atactccgct gcatttcagc
     5101 cgtttttatc cccagtacaa actgaatcgt ttaccaccta ccccggtaag cattctcgaa
     5161 agagcaaaaa aaattgccga aaattatggc ctgcaatatg tttatatagg taatgttccc
     5221 ggagcagagc aaggcaccac cttttgccca aaatgcaaaa cctctttagt agaaaggata
     5281 ggattcaagg taaaaaaaat ggtgatagaa aatggccgtt gtcctcgttg tggagctact
     5341 attcccggaa aatggtcggc ctgatcaatc gaaatccaaa ttccataaat ttcggtaatg
     5401 attcaacatg acaaaccatc aaattatata atattttact gagtactgcc atcattgttt
     5461 ttccatctgg cttttagcca aacagaacaa aaaagatcgt cagccatttt atatgggcga
     5521 tccaaaatcc catcttctat tttaaataaa aaaattcgac tttcctgtat gttttaacta
     5581 aagtttatta tctctttgaa aaggctttct tcactcatag attaattaga gtctgtctat
     5641 aaactcaaat tgttaataac tttttgataa ctaatta
//
LOCUS       DAOORU010000153         5659 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_17250_length_5659_cov_4.921306, whole genome shotgun sequence.
ACCESSION   DAOORU010000153 DAOORU010000000
VERSION     DAOORU010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17250_length_5659_cov_4.921306"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..139
                     /locus_tag="PLF20_08775"
     tRNA            58..139
                     /locus_tag="PLF20_08775"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:92..94,aa:Tyr,seq:gta)
     gene            145..217
                     /locus_tag="PLF20_08780"
     tRNA            145..217
                     /locus_tag="PLF20_08780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:178..180,aa:Gly,seq:tcc)
     gene            227..301
                     /locus_tag="PLF20_08785"
     tRNA            227..301
                     /locus_tag="PLF20_08785"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:259..261,aa:Thr,seq:ggt)
     gene            353..1540
                     /gene="tuf"
                     /locus_tag="PLF20_08790"
     CDS             353..1540
                     /gene="tuf"
                     /locus_tag="PLF20_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HOX81134.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHIDHGKTTLTAAITMVLSRAGLSEF
                     RSFDSIDNAPEEKARGITINTSHIEYQTANRHYAHVDCPGHADYIKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLARQVGVPRLVVFINKVDLVDDPELLDLVEMETRDLLNF
                     YGFDGDNTPIIRGSALGALNDEPQWVEAVKELMQKVDEWIPLPQRAIDKPFLMPVEDV
                     FSITGRGTVATGRIETGIIRTGDPVEIIGLGAEKLKSVVTGVEMFRKILDEGKAGDNA
                     GLLLRGIDKDEIRRGMVVAAPGSIKPHKHFKAQVYILKKEEGGRHTPFHNKYRPQFYF
                     RTTDVTGEITLPEGVEMVMPGDNLEIEVKLIYEIAMNPNLRFAIREGGRTVGAGQVTE
                     ILD"
     gene            1621..1693
                     /locus_tag="PLF20_08795"
     tRNA            1621..1693
                     /locus_tag="PLF20_08795"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1654..1656,aa:Trp,seq:cca)
     gene            1711..1914
                     /gene="secE"
                     /locus_tag="PLF20_08800"
     CDS             1711..1914
                     /gene="secE"
                     /locus_tag="PLF20_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOX81135.1"
                     /translation="MAKSKKIVRYVKESYDELVHKVTWPSWSELQNSAVVVSIASLII
                     ALVVFLIDIAFENILKFFYGLFM"
     gene            1976..2515
                     /gene="nusG"
                     /locus_tag="PLF20_08805"
     CDS             1976..2515
                     /gene="nusG"
                     /locus_tag="PLF20_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOX81136.1"
                     /translation="MSEEKKWYVIRAISGGERKVKQYLENEIKRLHLEDYISQVLIPT
                     EKVYQIRNGKKVCKERNYFPGYILIEAVLTGEIPGIILNIPGVLGFLGSKDKPQPLRK
                     SEVNRLLGKVDELAEAGEEVNIPFIVGETVTVIDGPFNSFSGVIEEINEEKKKLKVMV
                     KIFGRKTPLELSFMQVAKE"
     gene            2578..3021
                     /gene="rplK"
                     /locus_tag="PLF20_08810"
     CDS             2578..3021
                     /gene="rplK"
                     /locus_tag="PLF20_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOX81137.1"
                     /translation="MAKEVTGIIKLQIKGGAANPAPPIGPALGSKGLNIMEFCKQFNA
                     RTQDVAGKIVPVIITVYKDKSFDFVVKTPPVVVQLFEAVKVQKGSAEPNRNKIGSVTW
                     DQVHEIAAIKMPDLNCFTIESAMKMVAGTARSVGITITGNPPFAD"
     gene            3077..3772
                     /gene="rplA"
                     /locus_tag="PLF20_08815"
     CDS             3077..3772
                     /gene="rplA"
                     /locus_tag="PLF20_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOX81138.1"
                     /translation="MAQLTRKRKEALAQYNRNKAYTLTEACELVKKITYTKFDASVDM
                     DVRLGVDPRKPNQMVRGMVTLPHGTGKTIRVLVLCTPDKEEEAKAAGADYYGLDEYIN
                     KIKNGWTDIDVVITTPNVMPKVGALGKILGPRGLMPNPKSGTVTMDIATAVKEVKAGK
                     IEFKVDKYGIIHAPIAKVSFDANKIYENAKELLQAIIRLKPAAAKGTYIKSIYLSSTM
                     SPGIQVDPRSITA"
     gene            3812..4333
                     /gene="rplJ"
                     /locus_tag="PLF20_08820"
     CDS             3812..4333
                     /gene="rplJ"
                     /locus_tag="PLF20_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOX81139.1"
                     /translation="MRKEDKSRLIDVLTEQIKESNYFYITDASDLNCEITNQLRRLCF
                     KQNIQMKMVKNSLLKKAMERSGRDFSPLYEVLNGHTAVLFSETGSAPARLIQEFRQKT
                     KNPKPLFKGAYIEESFYIGEEQLDALANLKTKNELIGDIIALLQSPALNVISALQSGG
                     GKLAGIVKTLSER"
     gene            4413..4796
                     /gene="rplL"
                     /locus_tag="PLF20_08825"
     CDS             4413..4796
                     /gene="rplL"
                     /locus_tag="PLF20_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOX81140.1"
                     /translation="MADLKAFAEQLVNLTVKEVNELAKILKEEYGIEPAAAVAVAAAP
                     GASTGAAQETVEEKTSFDVILKDAGKEKLKVVKLVKELTSLGLKEAKDLVDAAPKPIK
                     EGISKDEAEGLKKSLEEVGAIVEIN"
     gene            4925..5629
                     /gene="rpoB"
                     /locus_tag="PLF20_08830"
                     /pseudo
     CDS             4925..5629
                     /gene="rpoB"
                     /locus_tag="PLF20_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212060.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
BASE COUNT         1770 a         1130 c         1233 g         1526 t
ORIGIN      
        1 cagctgattt gtaatcagct ggtcgggggt tcgagtccct ctatcggctc agtaaatggg
       61 gaggtaccag agcggtcaaa tggggcagac tgtaaatctg ttggctaagc cttcggaggt
      121 tcgaatcctt ccctccccac ttttgcggaa gtagctcatc tggcagagcg aaagccttcc
      181 aagctttagg tagcgggttc gaatcccgtc ttccgctctt agaaaagccg atgtagctca
      241 gcggtagagc acttccttgg taaggaaggg gtcacgagtc caattctcgt catcggctcc
      301 aacaatgaca cgttcatctt tgataacttt tttcaactaa taaatttttt ctatggcaaa
      361 agaaaaattc gatcgttcca aacctcatgt gaatattgga acaattggtc atatcgacca
      421 tggtaaaacc accctaaccg ctgctatcac catggtgctt tccagagcgg gtctttccga
      481 attccgttca ttcgattcaa tcgacaatgc accagaagaa aaagcacgtg gtataaccat
      541 taatacctcc cacatcgaat accaaacggc taacaggcat tatgcccatg tggattgccc
      601 cggccatgcc gactatatta agaatatgat taccggagca gcccagatgg atggtgctat
      661 cttagtagta gctgctaccg acggacctat gccccaaaca cgtgaacata tcctattggc
      721 ccgccaggtg ggtgttcccc gcttggtagt tttcataaat aaagtggacc tggtagacga
      781 tccagaactc ctcgatcttg tggaaatgga aactcgtgac ctcttgaatt tttatgggtt
      841 cgatggagac aataccccta taattcgtgg ctcggcactt ggtgcgctca atgatgaacc
      901 ccaatgggtc gaagccgtca aagaactgat gcaaaaagtc gatgagtgga ttcctttgcc
      961 tcaacgtgct atcgataagc ctttccttat gcctgttgaa gacgttttct ctattacagg
     1021 ccgtggaaca gttgcaacgg gtagaatcga gacgggaatc ataagaactg gagacccagt
     1081 ggagataatc ggactgggtg ccgaaaaatt aaaatcggtc gtaaccggcg tagaaatgtt
     1141 ccgtaaaatt ctcgacgaag gtaaagctgg cgataatgcc ggacttttgc ttcgtggaat
     1201 cgataaggat gaaattcgtc gtggcatggt tgttgctgct cctgggagca tcaaacctca
     1261 taaacatttc aaggcgcagg tgtatattct taaaaaggaa gaaggtggac gtcatacccc
     1321 cttccataat aaataccgac cccaattcta tttccgcact accgatgtga cgggagaaat
     1381 tactcttccc gaaggcgtcg aaatggttat gccgggcgat aacctcgaaa tcgaagtaaa
     1441 gttgatatat gaaatagcca tgaaccctaa ccttcgtttt gctatacgtg aaggaggcag
     1501 aacagtggga gcgggtcagg ttactgaaat attagactga ttcttttgtt ttatttcttc
     1561 atccaatcca agtccttgct tttcataagc gaggcctgtg ggaaagcaag aaaatgacga
     1621 acgggtgtag ctcaattggt agagtggtgg tctccaaaac cataggctgg gagttcgagt
     1681 ctctccaccc gtgcaaactt gcataaaatt atggctaagt caaagaaaat cgttcgttat
     1741 gtaaaggaaa gctacgacga attggtgcac aaagttacgt ggccttcctg gagtgaactt
     1801 caaaatagcg ctgtggtggt atctattgct tccctgatta ttgccctagt ggtttttctg
     1861 atagatattg ccttcgaaaa catcttaaag tttttctatg gtttgttcat gtgaacaaga
     1921 tgttagcgaa tgcgcagaaa aataccgaat ctattcctaa aataaaggag ggcgtatgag
     1981 cgaagaaaaa aaatggtacg taatacgagc cattagtggt ggcgaaagaa aggtgaagca
     2041 atacctcgaa aacgaaatca agaggcttca tcttgaggat tacatttctc aagtgctcat
     2101 acccactgag aaggtttatc aaattcgtaa tgggaagaaa gtttgtaaag aaagaaacta
     2161 tttcccgggt tatatactca tcgaagccgt attaactgga gaaattccgg gtattatcct
     2221 gaatatccct ggtgtattgg gttttcttgg gtctaaagat aaacctcaac ctttacgtaa
     2281 atcagaagtc aatcgtttat taggtaaagt tgatgaactt gccgaagcag gcgaagaagt
     2341 caatatcccc tttatagtgg gtgaaaccgt aacggtgata gatggtccat tcaatagttt
     2401 cagtggagta atcgaagaaa tcaacgaaga aaagaagaag ctaaaggtaa tggtgaaaat
     2461 ctttggccgt aaaactccac tggaattgag ttttatgcag gttgcaaagg aatagattat
     2521 acctcattgg catgctgaat tagcaagctt ttaacattta ctaaattatt cattacaatg
     2581 gcaaaagaag taaccggaat catcaaattg caaatcaaag gaggagctgc aaatcctgca
     2641 ccacccattg gcccagcctt aggatcaaag ggtttgaata tcatggaatt ttgcaagcag
     2701 tttaatgccc gtactcagga tgtagctggg aaaattgtac cagtcatcat tactgtttat
     2761 aaggataaat ccttcgattt tgtagtcaag acgccaccgg tagtggtaca gttattcgaa
     2821 gcagttaaag ttcagaaagg ttcggcagaa cccaatcgca ataaaattgg cagcgtaacc
     2881 tgggatcagg tacatgaaat tgctgcaata aaaatgcccg acctaaattg ctttacgatt
     2941 gaatcggcaa tgaaaatggt tgccggtacc gcacgcagcg ttggaattac tatcacagga
     3001 aatcctcctt ttgctgatta atattctgac attaattaaa taaagcactt gacatcaaac
     3061 catcaagttt taaaaaatgg cacaacttac acgaaaacgc aaagaagctt tagcacaata
     3121 taacaggaac aaggcttata cactgaccga agcctgtgaa ctcgtcaaaa agattactta
     3181 taccaaattc gatgcatcgg ttgacatgga tgtacgctta ggtgtcgatc ctcgtaaacc
     3241 caatcagatg gttcgtggta tggttactct tccacatgga acagggaaaa ccatacgtgt
     3301 attggtgcta tgtacacctg ataaggaaga agaagctaaa gcagctggcg ctgattacta
     3361 cggactggac gaatacatca ataaaataaa aaacggctgg accgatattg atgtcgttat
     3421 tactacaccc aatgtaatgc ctaaagtagg cgctttgggg aagattcttg gccctcgcgg
     3481 ccttatgccc aatccaaagt cgggtactgt tactatggat atagctacgg cagttaaaga
     3541 agtaaaagcg ggtaaaatcg aattcaaagt ggataaatat ggaatcatcc acgccccaat
     3601 tgccaaagtt tcgttcgatg caaacaagat ctatgaaaat gcaaaagaac tgcttcaggc
     3661 aatcatccga ttgaaaccag ctgccgcaaa gggcacctac atcaagagta tttatttgtc
     3721 gagcacaatg agcccgggca tccaagtcga tccccgttcg attactgcct gatgaataaa
     3781 atctatttca aaatgccaaa aaaaatgcat aatgagaaaa gaagataaaa gtcgactcat
     3841 agacgtattg actgaacaga tcaaggagag taattatttc tatattaccg atgcctctga
     3901 tcttaattgt gagatcacaa atcagttaag gcggttatgt ttcaaacaga atatccagat
     3961 gaaaatggtg aaaaacagct tgttgaagaa agccatggaa cgtagtggac gtgattttag
     4021 ccctctttac gaagtgctaa atggacatac tgcggttttg ttttccgaaa caggcagtgc
     4081 acccgctcgg ctcattcagg aattccggca gaaaaccaaa aaccctaaac cgctttttaa
     4141 gggagcatat atagaagaat cattctatat tggtgaagaa cagctcgatg cgctggcaaa
     4201 cctgaagacg aagaatgaat tgataggtga tatcatcgct ttgttgcagt cgcctgcatt
     4261 aaatgtgatt tctgctttgc agtcgggtgg aggcaaattg gcgggaatcg ttaaaaccct
     4321 ctcagaaagg tgatatttgg aattttaacc ctatgtaacc aaattttcaa attgtaaaac
     4381 aaattttaaa aacacttaat actcaattaa aaatggcaga tctgaaagct tttgcagaac
     4441 aattagttaa cctaactgtt aaggaagtca atgaacttgc aaaaattctt aaagaagaat
     4501 atggaataga acctgctgcc gctgtggcag ttgctgctgc tcctggggct tcgaccggtg
     4561 ctgcacaaga aaccgttgaa gaaaaaactt ctttcgatgt aattttgaaa gatgctggaa
     4621 aagaaaaatt aaaagtggtt aaattagtga aggaacttac cagcttaggc cttaaagaag
     4681 ctaaggacct ggttgatgct gctccaaaac ctattaagga aggcatctcg aaagacgaag
     4741 ccgaaggact taagaagtct ctcgaagaag ttggcgcaat tgttgaaatc aattagatac
     4801 tattctttgt tcctcctctg aaaactgccg tccttggcac acaaggacgg tggtttatct
     4861 cttttttcat gttactgtat tgttctttaa ttcattttcc gttcgaacta aaaaacctaa
     4921 taccttggcg cataaaaaca tagacaggat taacttcgga accacgaagc tcaccatcca
     4981 ataccctgat tttcttgatg ttcagttgca gtcgttcaag gattttttcc aactggaaac
     5041 aattcctgaa aaccgcgtca atgaagggct ttataaggtt ttttccgaaa atttccctat
     5101 ttctgatgcc cgcaacaact ttgtattgga atttcttgat tattttatcg atcctcccag
     5161 atattctatc gatgaatgtt tggaaagagg cttaacttac agtgttcccc tcaaggcgaa
     5221 actgaaattg tattgtaccg atcccgaaca cgaggacttc gaaactatta ttcaggatgt
     5281 gtatctggga atgattccat acatgactcc taagggaact tttatcatca atggagctga
     5341 aagagtagtt gtttcgcaat tgcatagatc gccaggagta tttttcggcc aaaactatca
     5401 cgccaatgga agtattctgt attcggcacg tatcatccct ttcaagggtt catggatgga
     5461 atttaccacc gatatcaata acgtgctcta tgcctatatc gatagaaaga aaaaattacc
     5521 cgttactact ctcctcagag ccattggata tgaatccgat agagatattt tagctatttt
     5581 cgacctattt caggaagtaa aggttacaaa aactacggca aagaaagtaa acaagcagta
     5641 attaacgtga gttttgaat
//
LOCUS       DAOORU010000154         5516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_17801_length_5516_cov_6.260758, whole genome shotgun sequence.
ACCESSION   DAOORU010000154 DAOORU010000000
VERSION     DAOORU010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17801_length_5516_cov_6.260758"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="PLF20_08835"
     CDS             <1..207
                     /locus_tag="PLF20_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX81141.1"
                     /translation="SNDRFIFELPASASYTIQITDISGRQIEKIETAGGTTQWDASAC
                     EPGIYLYRITSEGFLKTGKLVKQK"
     gene            698..1813
                     /gene="meaB"
                     /locus_tag="PLF20_08840"
     CDS             698..1813
                     /gene="meaB"
                     /locus_tag="PLF20_08840"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOX81142.1"
                     /translation="MDSKKKKCVNTLKVNEGIEPPPSINEELLKKPLKRKDIHPAEYY
                     VEGIFGGNRSILSQAITLVESSLKTHQKLAQKIIECCLKKAGNSVRIGITGVPGVGKS
                     TFIEAFGKQLTQTGHKVAVLAIDPSSQRSKGSILGDKTRMMKLSADPNAYIRPSPSAG
                     SLGGVARKTRGTIILCEAAGFDVIIIETVGVGQSETAVHSMVDFFLLLMLAGAGDELQ
                     GIKRGIMEMADAIVINKADGDNLLKAEQAKSQYESALHLFPPKTSSWSPKVVLCSSIY
                     QTGISQIWDMINEYINVVKTNGYFWENRKRQSLQSMDDTLHEYLLESFFSNPSIENKY
                     QQLQKKVMNSELSPYKAARQLFDLYLHILSTSKTIGD"
     gene            2159..2584
                     /locus_tag="PLF20_08845"
     CDS             2159..2584
                     /locus_tag="PLF20_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOX81143.1"
                     /translation="MMRWKNYPNLFDFFDSLFDREKEEVEGTNYYYTVPAVNIIETDK
                     AFELEVAAPGFSKSDFKITVENNVLRIASDVSKAKEKEEKKMECLRQEFAYGKFARSF
                     SLPNIINTEKISAEYKDGILHVILPKREELTISKQVAIQ"
     gene            2682..3221
                     /locus_tag="PLF20_08850"
     CDS             2682..3221
                     /locus_tag="PLF20_08850"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HOX81144.1"
                     /translation="MYRILFNALNGKRLYLIIPMVMMMGMISGCSIGGKIFSSKNEMK
                     KIEKQKETTAEAGVLDSVVKTWLGVPYRYGGTMRSGVDCSGLVREIYKEVYQIELPRS
                     TNDLLPIVQTVGEQNLQPGYLIFYEIEKEKEFHVGIYMGNRKFVHASSTKGVMISSMD
                     EPYFQQRFFKAGKIKNLSY"
     gene            complement(3321..4400)
                     /locus_tag="PLF20_08855"
     CDS             complement(3321..4400)
                     /locus_tag="PLF20_08855"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HOX81145.1"
                     /translation="MDNQSAVSKYSVFDDTLRFYNALLSDIENATKYIYIEIYRFSYH
                     GLGKKFRDALLKKVHQGVEVKILLDSWGTSPNHDFFSPLTKGGGEVRFFEKIRLNLDY
                     FTRSHRRNHRKIIVIDDEILYLGSANITDYNVVWRELIVRMEGELASVFKDIFLSMFE
                     IYNRYHLYSPELTAPISYGGFNIIRDAPSQRHQNIKKHLIKLIRSARKNIFIETPYFL
                     PSFLLRSALIKAARKGVKVFVILPKHSDVRLVDLLRNKYLGPLTEKGIKFFMYIPQNL
                     HSKLLLVDDNTFAFGSPNFDYRSFSFQHEILLSGHDQRLINLLKDHVNETLQDSEPFD
                     YLRWKRRPYIEKVFEWLIVPFRQLL"
     gene            complement(4407..5162)
                     /locus_tag="PLF20_08860"
     CDS             complement(4407..5162)
                     /locus_tag="PLF20_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOX81146.1"
                     /translation="MRVIIVDDEKLARDAIIEIGTRRIPDFEVVAEAASIAEAKQAIL
                     KYQPDLLLLDIQLLDGSGFDLLDQFSLPLNFKVIFITAYEEYAIKAFKASVLDYLLKP
                     IDADEFVTSINRAKFQLNLENFNKNLQTFIHLYHGYNSEQKQAIVIHSRSSLICLNHD
                     EILRIEGEGNHANIFLLSGELIRVSRTLNSFEEELKGKGFLQSHKSHLVNIRYIKTFH
                     SSELSLLMIDGTRVPVAFRRRSQVSYSIANWFK"
     gene            complement(5306..>5516)
                     /locus_tag="PLF20_08865"
     CDS             complement(5306..>5516)
                     /locus_tag="PLF20_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81147.1"
                     /translation="NLGLLHKFTTGDETSVLSLLHFIQFFCRSVVKKNIKSIYKTEEM
                     VKNGNYELIITVKTPVSQVVSSEVV"
BASE COUNT         1723 a         1017 c         1147 g         1629 t
ORIGIN      
        1 tccaatgata gattcatttt cgagctaccg gcttcagcct catatacaat acaaatcacc
       61 gacatttcgg gcagacagat agaaaaaata gaaactgccg gaggaactac gcaatgggat
      121 gcttcggcct gcgaaccagg gatttatctt tatcgcatca cttccgaagg ttttttaaaa
      181 accggtaagt tagtaaaaca gaaataaagc gaaatagcat aagaagaccg cgagacaggg
      241 agacttggga accgccaaag tcggataact tttttctcgc tgattgacgc agatttaatc
      301 cgctgattta cgctgatatt tttctgtgtg ctcctgcgaa agcttttttc tgcgatatcc
      361 ttagacatcg acacacacac taatcaccat ccactatcta acagtcccct tgtcccctcg
      421 tctcccagtc tcctcgtccc ctcctctcct cgtccccacg tctcccttct tccgttttcc
      481 gttttaaact ttatgaactt ttaacttatc catccaccat ccactaatca ccatccacta
      541 ataatttact caataatagt caatcttttt gaataaggga cattcccgaa aaactgggga
      601 aaattaattt caatttacag aatctttcta aagatgattt atcttagcaa aaacttaatg
      661 aaaagcaagt ttaattttgc acaacaaatt ccaatgaatg gatagtaaaa agaaaaagtg
      721 cgttaatacc ttgaaagtga atgaaggtat cgagcctcct ccttccatca acgaagaatt
      781 attgaaaaag ccgttgaaac gaaaggatat ccatcctgcc gaatattatg tggaaggaat
      841 ttttgggggg aaccgttcta ttctctctca agccattact ttagtcgaaa gttcactaaa
      901 gactcatcag aaacttgcgc aaaagattat cgagtgttgt ttgaagaagg caggaaactc
      961 ggtgagaatt gggattacgg gcgtgccggg agtgggcaag agtactttca tcgaggcatt
     1021 tgggaaacag cttacccaaa cagggcataa ggttgccgtt ttggccatcg atcccagcag
     1081 tcaacgctcg aaaggaagta tcttgggcga taagacccga atgatgaagt tatcggctga
     1141 tcccaatgct tatatcaggc cttctccttc ggcaggttcg cttggaggtg ttgcccgtaa
     1201 aactcgtgga acgattattt tatgcgaagc agcaggattt gatgttatca tcatcgaaac
     1261 cgttggtgta gggcaatcgg aaactgccgt tcattctatg gtcgattttt ttctgttatt
     1321 gatgctggcc ggggctggcg atgagctcca aggcatcaaa agaggaataa tggaaatggc
     1381 cgatgcaatc gttatcaaca aggcagatgg agataatctc ctgaaagccg aacaggctaa
     1441 aagtcaatac gaaagtgcac tgcatctttt cccacctaaa acatcgagct ggtcaccaaa
     1501 agttgtactt tgttcctcta tctatcaaac aggcataagc caaatatggg acatgatcaa
     1561 tgaatacatc aatgtggtca aaactaatgg atatttctgg gaaaaccgta aacgtcaatc
     1621 tcttcaatca atggacgaca cacttcatga atacttgctc gagtcgtttt tttcgaatcc
     1681 ttccatcgaa aacaaatacc aacaattaca aaaaaaagtg atgaatagtg agcttagtcc
     1741 ctataaggca gctcggcaac ttttcgatct ctacctgcat atattgagta cttcgaaaac
     1801 aataggagat tagtgaaggg aggaaggaga ggagactggg agacaaggag acggagagac
     1861 aaggagaaaa gaaatgttgt tatttgtaat ttgaaaaagt atcattgtct aaatgatgct
     1921 gatcgttttt gactttagca atcatctgta aagaagcaaa aaataatatc cattgattac
     1981 taatcgattg atttgatttt tttataaaca cgacattaag gcagtaagca taatggattt
     2041 atatgccata atggcgcaaa ttattttact ttttctcatg gcacaggaat tgaccttttt
     2101 ttttcgcaaa taaatacaat gtctaacatt aaaaattagg gagggaaaaa ttatggctat
     2161 gatgcgttgg aaaaattatc ctaatctctt cgactttttc gatagtcttt ttgatcgtga
     2221 aaaagaggaa gtagaaggca ccaattatta ctacactgtt cctgccgtca acatcatcga
     2281 aaccgataaa gctttcgaac tcgaagtagc tgctcctggt ttctcgaaaa gcgatttcaa
     2341 aattacggta gaaaacaatg tattgaggat tgcatcggac gtttcgaagg ctaaagaaaa
     2401 agaagaaaag aagatggaat gcttacgtca ggaatttgct tatggcaaat ttgctcgttc
     2461 attcagtctt cccaatatca tcaataccga aaagatttcg gccgaataca aggatggcat
     2521 tttgcatgtg attctgccta aacgtgaaga attgaccatt agcaaacagg ttgctatcca
     2581 gtaagtttcg agatccttaa tgagtgggat gtttcgctga aacatcccat ttttttatta
     2641 ttgcatgacg aatgtttgcc atgcaagatt actgtttatc aatgtataga attctcttca
     2701 atgctttgaa tggcaagaga ttatatttaa taatcccaat ggttatgatg atggggatga
     2761 tttcgggttg ttcgattggt gggaagattt tttcgtcgaa aaacgagatg aaaaaaattg
     2821 agaaacagaa ggaaacgact gcagaagctg gagttttgga tagtgtggtt aaaacatggc
     2881 ttggggttcc ttatcgctat ggaggtacaa tgagatctgg tgtggattgt tcgggtttgg
     2941 tacgtgaaat atacaaagaa gtttatcaga tcgaattgcc acgttctact aacgatttgc
     3001 tccccattgt tcaaactgtt ggtgagcaaa atctgcaacc cggttatctg attttttatg
     3061 aaatagaaaa agaaaaggaa tttcatgttg gcatttatat gggtaatcgc aaattcgtgc
     3121 atgcttcatc cactaaagga gtgatgatca gcagtatgga tgaaccttat tttcagcaga
     3181 gattttttaa agccgggaaa ataaaaaacc tttcttattg aaaaggccgt agagtatttc
     3241 ccatcttccc tcgagatcca acgatgaatt ctcatattga aagaggtcat tcaataatga
     3301 acaaaatcaa ttccttttat ttagagtaat tgtctgaaag gaacaatgag ccattcgaaa
     3361 accttttcga tgtatggtcg tcttttccat ctcaaataat cgaagggttc tgagtcctgt
     3421 aaggtttcgt tgacatgatc tttcaaaaga tttataaggc gctggtcgtg gccgctcagc
     3481 aaaatttcgt gttgaaagct gaagctgcgg taatcgaagt tcggtgaacc aaaggcaaag
     3541 gtgttatcgt cgaccagtaa gagttttgaa tgaagatttt gagggatata cataaaaaat
     3601 ttgattcctt tttcggttaa ggggccgaga tatttatttc gaagcaagtc gactaaacgg
     3661 acgtcggaat gtttggggag aatgacaaaa accttgactc cctttcgggc agcctttatc
     3721 aaagctgaac gtaagaggaa agaaggcaga aagtagggag tttcgataaa tatattcttg
     3781 cgagccgagc gaattaattt tattaagtgt tttttgatgt tctggtggcg ttgcgagggt
     3841 gcatcgcgaa tgatgttgaa tcctccgtaa gaaatgggcg cggtaagctc gggcgagtaa
     3901 agatgataac gattgtatat ctcgaacatg gataggaaga tgtctttgaa aacactagca
     3961 agttcgcctt ccattcttac gatgagttcg cgccagacaa cgttataatc ggtgatattg
     4021 gccgagccta gataaagaat ctcatcgtcg atgacaatga ttttgcggtg attgcgacga
     4081 tggctacgcg taaaataatc cagattgagc cttatcttct cgaagaacct tacttctccg
     4141 ccgccttttg tgagtgggga gaaaaaatcg tgattggggc tggttcccca cgaatctaat
     4201 agaattttaa cttcaacacc ctgatgaact ttcttcaata aggcatctct gaactttttc
     4261 ccaagaccat gataactaaa acggtaaatc tcgatataaa tatattttgt tgcattttct
     4321 atatccgata ataaagcatt ataaaacctc agagtatcgt cgaaaacgga atatttactg
     4381 acggcagatt gattgtccat aagttgttac ttaaaccagt tagcaatgga gtaagatacc
     4441 tgtgaacgcc tgcgaaatgc aaccggaacc cttgtgccgt caatcattaa taaacttaat
     4501 tcactggaat ggaaggtttt gatgtaccga atatttacca gatgcgattt atgcgattgt
     4561 aaaaaaccct tccctttcaa ttcttcttca aaactgttca aggtacggga tactcttatt
     4621 agttcgccag ataataaaaa tatgtttgcg tggttgcctt ctccttctat acgcagaatt
     4681 tcatcatggt tgagacagat gagcgatgat ctggaatgaa tcactatggc ctgtttttgt
     4741 tcactattgt aaccatgata aagatggatg aaagtctgga ggtttttatt aaaattttcg
     4801 agattcaact gaaatttagc cctattaatg cttgttacaa attcatcggc atcgataggt
     4861 tttagcaaat aatccagtac acttgccttg aatgccttta tggcatattc ttcgtaagcc
     4921 gtaataaaga tgactttaaa attcaaaggc agagagaatt gatctaacaa atcgaaacca
     4981 cttccatcga ggagctgaat atcgagcaaa agcagatcgg gctgatattt caggatggcc
     5041 tgcttggctt cggcaatgct ggcagcttcg gcaactacct cgaaatcggg tattcggcgc
     5101 gtacctattt caatgatggc atcacgtgct aatttttcat cgtcaactat gattactcgc
     5161 atgctgacca agtggttttt tgaatggata aaattagaaa aatattatga caacataaat
     5221 ggatttaatt aaaatcacta gatgacttga tggttcatga agaaagaaaa gaggagtggc
     5281 cgtatcaatc taggttgatt ctgtattata ccacttcgct acttactact tgcgaaacag
     5341 gtgtttttac cgtaataatg agctcgtaat tcccgttttt taccatctct tctgttttat
     5401 agatggattt aatattcttt ttcactactg accgacaaaa aaattgaata aaatgaagga
     5461 gggacaaaac cgaagtttca tccccagttg taaatttgtg tagtaaacct aaatta
//
LOCUS       DAOORU010000155         5475 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_17960_length_5475_cov_6.485055, whole genome shotgun sequence.
ACCESSION   DAOORU010000155 DAOORU010000000
VERSION     DAOORU010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17960_length_5475_cov_6.485055"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PLF20_08870"
     CDS             complement(<1..317)
                     /locus_tag="PLF20_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81148.1"
                     /translation="MKRTCFLILMAALVGFIQGCVKDEVFQGPPKIKSVALNPVSPGE
                     GQVVTVMAKVTDDAGVKSVNLYYKTTADFTKVAMQADTANFYKGQIPGQNKDITVSYY
                     IEA"
     gene            complement(347..1714)
                     /locus_tag="PLF20_08875"
     CDS             complement(347..1714)
                     /locus_tag="PLF20_08875"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOX81149.1"
                     /translation="MNRKLSYLFSALLMLIGIPLWAQKEADTIRYNQYGVAVDRIPLI
                     AERRGGVLVFESRDQKQRIWFDTRVQVDGAIFTGDTYNEIGNGTSIRRARLAMKTELN
                     EKWYGELDLDFANSELELKDAYLAYIPNEHWIIRTGNFKESFSMESTTTSRYLTFMER
                     PMAVSAFAPSRHIGMALQYNYNWLLAIGGIYFQDVSDLEERTYSVDNNKNLGIDEGYS
                     LTGKLVFMPLNNKSGYGIHLGASTSYRTPKTDAEIPGSVRFSTRSLTSINRKKYLDTD
                     NILNVDYTWQNGFELAAYYHNFRIQGEYLLTDVHRKNDLPTENFNGFYVMGSCMLFGG
                     EYRYNQSDGEFTQPSRGRKWGDIELALRYDMLDLNSRMDGVMGGAGEGITAGINYHVN
                     SSIKVMFNYAYLNHDRYANGKGKLFVGHDILGNLTKNPSDVVEAEGKAGEDFSMFSIR
                     FEIDF"
     gene            complement(2523..3209)
                     /locus_tag="PLF20_08880"
     CDS             complement(2523..3209)
                     /locus_tag="PLF20_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007751643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOX81150.1"
                     /translation="MIEKKSPKADLEEKRGIFVEIGLVIALGLILVAFEWKTYEKKQV
                     EVFQRQAIDIPEEMVEITKQETPQAAPPPPQQVTVLNIVDDNANIETDFTIDAEATQS
                     TVVQEYIPVQNISKEEEHIEEQEIFTIVESMPEFPGGEEARTRFLLENIKYPQMARES
                     GIQGVVYVTFVVEPDGSISNVRVIRGIGGGCDEEAIRVVKMMPKWIPGNQRGKPVRVQ
                     FNMPIKFTLQ"
     gene            complement(3374..3757)
                     /gene="gcvH"
                     /locus_tag="PLF20_08885"
     CDS             complement(3374..3757)
                     /gene="gcvH"
                     /locus_tag="PLF20_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008661521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOX81151.1"
                     /translation="MNILDNLKYTRDHEWIKVEGNSAWIGISDYAQSQMGDIVYVEVE
                     TEGEILNKGESLGSIEAVKTVEDIYMPVSGEIVEFNEKLIEKPDLLNKDPYGEGWIVK
                     ITLINPSELDDLLDSQSYKEFIESL"
     gene            complement(3918..4487)
                     /gene="gmk"
                     /locus_tag="PLF20_08890"
     CDS             complement(3918..4487)
                     /gene="gmk"
                     /locus_tag="PLF20_08890"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOX81152.1"
                     /translation="MNRKMLIFSAPSGGGKTTIVKAVLKQFPELAFSVSTTSRPQRPN
                     EINGVDYYFISTEEFRKKIANEEFIEWQEVYPGIYYGTLKSELERIWALGKNPVFDID
                     VKGALNLKNIFQSESLTIFIQPPSLQILEQRLRNRGTETEESLARRLGKAAYELTFAT
                     YFDAVIVNDQLQQAIQEAIHLVADFLHSI"
     gene            complement(4492..5370)
                     /locus_tag="PLF20_08895"
     CDS             complement(4492..5370)
                     /locus_tag="PLF20_08895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOX81153.1"
                     /translation="MIQSMTGFGKTQVLAKGKNITIEVRSLNSRQADLNLKLPVIYRE
                     KEYEIRNTVIDSLERGKIDVSINVDFTNSSHAAILNMAVAKNYFDQLKTLEKELGLDR
                     VQTDYIGIISRMPEVFRMIDSEINQEEWDLLIGGLKDALCQVIGFRKEEGSVLEKDIG
                     QRIGCIEKLLQSIQPYEEERIDLVRNKILKELMALPIQPSYDKNRFEQEMIYWLEKID
                     FTEEKTRLSKHLHHFLRTLEEESSQGKKLGFIAQEIGREINTLGSKANHAEIQRIVIS
                     MKDELEKIKEQLLNVL"
BASE COUNT         1598 a         1110 c         1005 g         1762 t
ORIGIN      
        1 tcagcttcaa tataatagga aaccgtaata tccttgtttt gtcccggtat ctggcccttg
       61 tagaaatttg ctgtatcagc ctgcatggca acttttgtaa aatcggcagt ggttttataa
      121 taaagattga ccgatttaac tccagcgtcg tcggtaactt tggccatgac ggtaaccacc
      181 tgtccttcac caggtgaaac tggattgaga gcaaccgatt tgattttagg agggccttga
      241 aatacctcat cttttacgca gccttgtata aatcccacca atgctgccat taaaattaaa
      301 aagcatgttc ttttcatatt tcaaaaaggt taagagagtt gaacaattaa aaatcgattt
      361 cgaaacgaat ggaaaacatc gaaaaatctt ccccagcttt cccttcggct tcaaccacat
      421 cggaaggatt ttttgtaaga ttgcctaaaa tatcatgtcc tacgaatagt tttcctttcc
      481 cgttagcgta tctgtcgtga ttgagataag catagttgaa cataaccttt atgctggaat
      541 ttacatgata attgatacct gctgtaattc cctcgccagc tccgcccatt acgccatcca
      601 tgcgactgtt gaggtctaac atatcgtagc gaagggcaag ttctatatca ccccatttcc
      661 ttcctctcga aggttgggta aactcgccat cactttgatt atatcgatat tcacctccaa
      721 aaagcataca gcttcccatg acataaaatc cgttgaagtt ttcggtgggc aaatcatttt
      781 tgcgatggac atcagtcaat aggtattctc cctgtatcct gaaattatga tagtaggccg
      841 ccagctcgaa accattttgc catgtataat ccacattgag tatgttatcg gtatcgagat
      901 atttcttgcg attgatacta gtaagagaac gcgtactgaa acgaacactc cccggaatct
      961 cggcgtcggt tttgggagta cggtaagaag ttgatgcgcc taaatgaatc ccatagccag
     1021 atttattgtt aaggggcatg aagacaagtt tacccgtaag ggaatatccc tcgtctatac
     1081 caaggttctt gttattgtcg accgaataag ttcgttcttc cagatcactt acgtcctgaa
     1141 agtagatgcc tccaatagcc agtaaccaat tgtaattata ttgcaacgcc ataccaatat
     1201 gacgtgaagg agcaaaagcc gatacagcca ttggcctttc catgaaagtt aagtagcgag
     1261 aggtggtagt agattccatc gaaaagcttt ccttgaagtt accagttctt attatccaat
     1321 gctcgttagg aatgtatgct aggtaagcat ccttgagttc gagttcagaa ttggcaaagt
     1381 caaggtctaa ttccccatac catttctcgt ttaattcggt tttcatggcc agacgagccc
     1441 ggcgtatcga tgtgccattc cctatctcat tgtaagtatc accggtaaat atagcgccat
     1501 ctacctgaac gcgtgtatcg aaccagattc tctgcttctg gtcacgactt tcgaatacaa
     1561 gtactccacc tctgcgttct gcaatcagtg gaatacgatc tacagcaaca ccatactgat
     1621 tgtatcgaat ggtgtcagcc tccttttgag cccacaaggg aattcctata agcatgagaa
     1681 gggcagaaaa taaataagag agttttcggt tcataagatt ttatttttaa agtgagggat
     1741 tcatgatggc aaatttaagt aaaatagcag attattagta agataatgct tattccgata
     1801 aaatatttcc atgtaaggta acttcaattt taagaaaagg ttccgaaaaa caacacgata
     1861 ttcttttcat cggattttca aatttacggt tataatcatt atcaatactt taaagaccaa
     1921 atatcaacaa ttttttactc cccaaacaag gtattttcat agcatagaca atatggccgt
     1981 tctattcgaa aattattggt aagtgttagc aagttgtaat cctaagacga aatatcaacc
     2041 atctttcccc aattgattga ttaacaatat attttcaaaa tttcaaactt ttacaggcta
     2101 ctaaaagaag cttattattt aacacttttt tttacgccga tatatccaat tttttatgaa
     2161 accattgata tacatggcga cttcatccat aatggtttga aacaacaaat ttcattttct
     2221 attttcgaca aggcgtggaa tactaagtgg tacgcggtgc ttagtacatg gcgaatggga
     2281 gatggtggtg agtgaaaaaa ggcgaaagat gttgcgtgtg gcggggcatt ttggaggttg
     2341 gtgtaaaaaa tgaaagttgt gtaaggggta taatgcaatg ttatagttta aggtatgtat
     2401 cgaacagccg aacgagaatg aataaggccg ggatattttt aaaaaatttc caaattgcaa
     2461 acaaaaaaac cggctctgat tttataatga gtcggttttg ggtgactatg cgaataataa
     2521 ttttattgca gggtaaactt tatgggcatg ttaaattgta cacgtacagg ctttcctctt
     2581 tgattaccag gaatccattt tggcatcatt tttacgactc gaatagcttc ttcatcacat
     2641 ccgcctccta tacctctgat taccctaaca ttacttatgc taccatcggg ttctacgacg
     2701 aaagttacat aaaccactcc ctgtatccct gattcacgtg ccatttgagg gtatttaata
     2761 ttttcgagta aaaatctcgt acgggcttct tcacctcctg gaaattcggg catggattct
     2821 actattgtaa aaatttcttg ctcttcgata tgttcttctt ctttgctgat attttgtaca
     2881 ggtatgtatt cttgtacaac agtactttgc gttgcttcgg catcaatggt aaaatctgtt
     2941 tcgatattgg cattatcgtc aacgatattc agaaccgtaa cctgttgagg tggtggaggt
     3001 gcagcttgag gggtttcctg tttggtaatt tcgaccattt cttctggaat atctatagcc
     3061 tggcgctgga atacttcaac ttgctttttc tcataagttt tccattcgaa ggcaactaaa
     3121 ataagcccca acgcaatcac cagaccaatt tctacaaaaa ttcctctttt ttcttctaag
     3181 tcggctttgg gcgatttttt ctcaatcata agacgaaatt tttaaattat ctgctaaaca
     3241 aataacgtgc taaattactc actatttctt ttcaatgaag aaaattttcg aatttttcac
     3301 tttttctttg atcataatag gatgagagtt gaaaaattca accccataga ataggataac
     3361 aagaagctct ggttcagaga gattcgatga attccttata agactggctg tcgagcagat
     3421 catccagttc gctggggtta atcagagtga tttttacgat ccagccttcg ccataagggt
     3481 ctttattcaa caaatcgggt ttttcgatga gtttttcatt aaactcgaca atttcacccg
     3541 aaacgggcat ataaatatct tcgacagttt ttactgcttc gatgcttccg aggctttctc
     3601 ctttgtttag aatttcaccc tcagtttcca cctctacata aacaatatca cccatctggc
     3661 tttgggcata atcggaaata cctatccatg ccgaattgcc ttccacctta atccattcat
     3721 gatcacgagt gtactttagg ttatctaata tgttcatttt attgtttttt gattttcgat
     3781 ttgcttgcaa atataaagtt ttctttcaag tggtgataca agaaataaaa tttcccaaaa
     3841 atcaaataaa gaagcatgaa aggttcaaaa cttcataaac ataatttctg taatctgtag
     3901 aaaaaacaaa tttggattca aatggaatgc aagaaatctg cgaccaggtg aatggcttcc
     3961 tggatagctt gctgcaattg atcgttcacg ataacagcgt cgaagtaagt ggcgaaggta
     4021 agctcgtagg cagccttgcc caaacgtcga gcaagcgatt cttcggtttc ggtgcccctg
     4081 ttgcgcagcc tctgttccaa aatctgtaac gagggtggct gaataaaaat ggtcaaggat
     4141 tctgattgga aaatattctt caaattgaga gcacctttca catcaatgtc gaacacagga
     4201 tttttgccta gggcccaaat tctttctaac tcgcttttca gagttccata gtaaattccg
     4261 ggataaactt cctgccattc gatgaattcc tcgtttgcaa ttttctttcg aaattcttca
     4321 gtactgatga aataataatc cacaccattg atttcatttg ggcgctgtgg ccggcttgtg
     4381 gtggaaaccg aaaaagccag ttcggggaat tgtttcaaaa cagccttgac gatggtcgtt
     4441 ttacctccac ccgaaggagc tgaaaatatc agcatcttcc tgttcatgac ttcataagac
     4501 atttaaaagt tgttccttga tcttttcgag ttcatctttc atagatatga cgattctctg
     4561 tatctcggca tgattggcct tcgatcccaa tgtattgatt tctcgcccaa tctcctgggc
     4621 aataaaacca agttttttcc cctgtgatga ttcttcttcg agcgttcgaa gaaaatgatg
     4681 cagatgcttg gaaagacggg ttttttcctc tgtaaaatca attttctcga gccaataaat
     4741 catttcctgc tcaaaacggt ttttatcata agatggctga atgggcaaag ccatcaattc
     4801 cttcaaaatt ttgttgcgca ccaaatcgat gcgttcctcc tcgtagggtt gaatgctttg
     4861 aagcaatttt tctatacatc caattcgctg gccaatatct ttttcaagaa ccgaaccttc
     4921 ttcctttcta aagccaatta cttgacacaa ggcatccttc agacctccta tcaaaagatc
     4981 ccattcttct tgatttatct cggaatcaat cattctaaat acttcaggca ttcgcgaaat
     5041 aataccaata taatcggttt gaactctatc aaggccgagt tccttttcga gagtttttaa
     5101 ctgatcgaaa taatttttag ccacggccat attcaggata gctgcatgtg aagaattagt
     5161 aaagtcaaca ttgatagaaa catcgatttt ccctctttcg agagaatcaa tgacggtatt
     5221 tcgaatttca tattcttttt cgcgatatat cacaggaagt ttcaagttta aatcagcttg
     5281 cctgctattg agtgaacgga cctcgatggt aatgtttttt cccttggcca aaacctgagt
     5341 tttcccaaaa cccgtcatgg attgtatcat ttcatttgag ctttatctgc aaaattaaag
     5401 ggaattatat taaatagtat tattaaccga caaaattcta aaagttccta aacatttttt
     5461 atataccact tattc
//
LOCUS       DAOORU010000156         5453 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_18039_length_5453_cov_4.693405, whole genome shotgun sequence.
ACCESSION   DAOORU010000156 DAOORU010000000
VERSION     DAOORU010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18039_length_5453_cov_4.693405"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="PLF20_08900"
     CDS             <1..487
                     /locus_tag="PLF20_08900"
                     /inference="COORDINATES: protein motif:HMM:NF020133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOX81154.1"
                     /translation="IVNPLRIHQRYLDELTHDLVLFYTQTRRDSSRIIENQAEAIASH
                     HQLRTKATIKLKEQAFMMKEALLKGELRRLGEILNFGWEHKKQIADGITNPQIDSIYS
                     TALQNGAIGGKISGAGGGGFMMFYCPENHRYRLAEALKPFGGFVVNYEFTQQGLSTWS
                     F"
     gene            508..1101
                     /locus_tag="PLF20_08905"
     CDS             508..1101
                     /locus_tag="PLF20_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HOX81155.1"
                     /translation="MEKFIISRIEESIVLKQNIINNRELLQNLALLTEACIRCFKEGG
                     KVLFCGNGGSAADAQHLAAELSGKFYFDRPPLPSEALHVNTSFLTAVANDYAYEEVFA
                     RMVRATGNKGDILIGLSTSGNSTNILRAMEAANEKAITTAALTGETGGKLKNLVHYLI
                     NVPSKDTPRIQEAHILLGHILCEQIEKELFINPAHPH"
     gene            1102..1809
                     /locus_tag="PLF20_08910"
     CDS             1102..1809
                     /locus_tag="PLF20_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOX81156.1"
                     /translation="MIKEVIILAGGLGTRLRSALPDMPKCMAPVNGKPFLEYVMDYLE
                     MYGVKKIILATGYLHEKIEEHFHNIYKYMNIEYSREDEPLGTGGAVKKALEKAEGKQV
                     VVMNGDTMFRVNLGKLYDFTLIMKSDLTIVVRQVNDVTRYGTVEFDETGRITAFREKG
                     SMEGSGWINGGTYVIHKDFFLRQALPEKFSLEKDLFEKIYLKHPVYAHKCYQYFIDIG
                     IPEDYERAQEEFRAFEF"
     gene            1769..2368
                     /locus_tag="PLF20_08915"
     CDS             1769..2368
                     /locus_tag="PLF20_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOX81157.1"
                     /translation="MNERRKSLELLNSDRKFALFLDRDGVINRRIVGGYVTRPAEFEF
                     LPGALEAMSIFSKLFYPIIVVTNQQGIGKGKMTEEQLNEIHSFMKEEIRKAGGEIHAI
                     YHCPDLKVSHSTCRKPRPGMALRARHDFPALNFKHSMMVGDMISDMVFGHNLGMITVL
                     IADEQLARKHPMLIDYRFNSLYDFAMIISKFDVQEPPKK"
     gene            complement(2420..4240)
                     /locus_tag="PLF20_08920"
     CDS             complement(2420..4240)
                     /locus_tag="PLF20_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX81158.1"
                     /translation="MLKLRLIFILFLLGMFLPYGKGETSKEIASSKNVGIIKRIDSLL
                     LASENLTNSPERMLFLSMKADSLSKKFAYPYGMISSSYNIARAMFYQSRLKESYKLLD
                     SLLIAIESDSAAISKVMNYQVGRSKIYSLMAIIFQELDEYERSMDYYFKALKLIEKGG
                     QSYDVALIYKGLGGLNLKAGNFGKANEYFNKALIASNKFADPQIKFDIYQEQYEYFKN
                     RKEYSKALEFSVKMIGLSKTAEIPYMTAIALKNSGEIYYLLDEPSLAKAYLYEVIHNE
                     SYEEFPNVLSECYAILARLFFDESEYTEAEGYAQKAYEKAFNTSRLSLKANALYELAK
                     SQQAIGKFSEAFDHLKLHLLYKDSLNNINNTHKILQIYSKAELDKVMTEKKLLENQLT
                     IKMLESSRKGHLLIGSILIILLLSSLALIMVKKYKFEKEVNKKLERQQAIINEQELII
                     EKEKENQLKIELEHKNRELITHTMLLTQMHEDKVKLIDELQEIYSKVSKVDKDSASVI
                     AGRINALRNSINANTWEDFKTYFENVYTDFYSNLLAAYPNLTPNEKKLCALLRLNLNT
                     KEIAAITSREVRSIESARTRLRKKLGLSPNTNLSIFLSKF"
     gene            complement(4313..>5453)
                     /locus_tag="PLF20_08925"
     CDS             complement(4313..>5453)
                     /locus_tag="PLF20_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX81159.1"
                     /translation="IATYSNSFTTDMIIRLIKVNEIGDTLWTSILPNSNGCNLTLAGC
                     LTQTFDGGYMVAAYGWRPPNNNQIVIFKTDNEGNYEWFKEYGGSTNDDYGACIKPTLD
                     GNYILAGYTYSFGSGLCDGYLIKIDPNGDTLWTSVVGGSSYDSFHFVQPTSDGGYIAV
                     GSTQSYGNKEQGFTVKVDNKGKVEWISAQGGNQNEGFEGVVETLNHEYLITGNTSSYG
                     NGQHDFIIVLMDDQGNMLDMKTYGTNGDDFGSTIEIMRDNRYIAGGSKTKNNSLDSWA
                     LCFKADSIVGIDLPRSSQTVISLEISPNPFYNQANIDIFVHSSGMISLNLFDMNGVKV
                     DEIFDGYISAGAHSFQYKNASLSRGIYFCQLKIENQIVIKKVILL"
BASE COUNT         1718 a         1045 c          976 g         1714 t
ORIGIN      
        1 catagttaat ccacttcgta ttcatcaaag atatttagat gaacttacgc acgatttggt
       61 gctattttat acacaaaccc gtcgcgattc gagccgtatc atcgaaaatc aggcagaagc
      121 cattgccagt catcatcagc ttcgtaccaa ggccactata aaattgaagg aacaggcttt
      181 tatgatgaaa gaagctctat tgaagggcga attgagaaga ttaggcgaaa tcttgaattt
      241 tggctgggaa cataagaaac aaatcgctga tggaattacc aatccacaaa ttgatagtat
      301 ctatagcact gctttacaaa atggagctat tggaggtaaa atttcaggag ccggtggtgg
      361 tggattcatg atgttctatt gtcccgaaaa tcatagatat cgattggctg aagctcttaa
      421 acctttcgga ggttttgtcg tgaattacga attcacccaa cagggtttgt ctacttggtc
      481 gttttaatta aatattttac ttgaagtatg gaaaagttta ttatttcaag aatcgaagaa
      541 tctatcgtcc tgaaacagaa tatcatcaac aatagagagc ttttgcaaaa tcttgctctt
      601 ttgacagaag cctgtattcg atgtttcaag gaaggaggca aggtactttt ttgcggcaat
      661 ggcggaagtg cagccgatgc gcaacacttg gccgccgagc tttcgggcaa attttatttc
      721 gaccgccctc ccctgccctc cgaagccctt catgtcaata cctcattttt aacagccgta
      781 gccaatgact atgcctacga agaagtcttc gccagaatgg ttcgtgccac tgggaacaag
      841 ggtgatatac tgattggcct ttctacttca ggcaattcga cgaatattct tcgagccatg
      901 gaagcagcaa atgaaaaagc aatcacgaca gctgccctca cgggtgaaac tggaggcaaa
      961 ctgaaaaacc tcgttcatta tcttatcaat gtaccttcga aagatacacc ccggattcaa
     1021 gaagcacata ttctactcgg acacattttg tgcgaacaaa ttgaaaaaga actgtttatc
     1081 aaccctgctc atcctcattg aatgataaaa gaagtcatta ttctggccgg tggattaggt
     1141 acccgacttc gttcggcttt acccgatatg cctaagtgca tggcccccgt caatggaaaa
     1201 ccttttctgg aatatgtgat ggactacctc gaaatgtatg gagtaaaaaa aattatattg
     1261 gctacaggct atttgcatga aaaaatcgag gaacattttc ataacatata caagtacatg
     1321 aatatagaat actccagaga agatgaaccc ttaggaaccg gaggtgcggt gaaaaaagca
     1381 ttggagaaag cagaaggcaa acaagtagta gtaatgaatg gtgataccat gttcagggtg
     1441 aatttgggaa agttgtatga ttttaccctc ataatgaaat cagatcttac gattgtggtg
     1501 cgacaggtaa atgatgttac cagatatgga actgtcgaat ttgacgaaac aggccgtatt
     1561 acagcttttc gagaaaaagg aagcatggaa ggctcaggtt ggattaatgg tggaacttat
     1621 gtcatccaca aggatttctt ccttagacaa gccttgcccg aaaagttctc actcgaaaaa
     1681 gacttattcg aaaaaattta tctcaaacat cctgtttatg cccacaaatg ttaccaatac
     1741 tttatcgaca ttggaattcc agaagattat gaacgagcgc aggaagagtt tagagctttt
     1801 gaattctgat agaaaatttg ccctttttct cgacagagac ggtgtcatca accgaaggat
     1861 agtgggggga tatgttaccc ggccagctga attcgaattt ttacccggag cattggaagc
     1921 aatgagcatt ttttccaagc tgttctatcc catcatagta gtgactaatc agcaaggcat
     1981 aggaaaaggc aagatgacag aagagcaatt aaacgaaata cattccttta tgaaagaaga
     2041 aatacgcaag gctggaggtg agatacatgc catatatcat tgccccgatc taaaggtttc
     2101 acactctact tgtcgtaagc cgcggcctgg aatggcttta cgcgcccgac acgattttcc
     2161 tgcattaaat tttaaacatt cgatgatggt gggcgatatg ataagtgata tggttttcgg
     2221 acataatctg ggaatgatca cagttttaat tgcagatgaa caacttgcaa ggaagcaccc
     2281 tatgttaatt gattatcgat ttaacagcct ttacgacttc gctatgatta tttcgaaatt
     2341 cgatgttcag gaaccgccaa aaaagtaaag aattaatgta agtaaacggc attctgaata
     2401 tttatgcttt taaagagagt taaaacttgg aaagaaaaat cgaaagattg gtattaggag
     2461 aaagacccaa tttttttctc aatctggtcc gtgccgattc tatgcttctt acttcacgtg
     2521 aagtgatagc tgcaatttct tttgtgttaa gattcaatct gagcagagcg cataattttt
     2581 tttcgttagg ggttaagttt gggtaggcag ccaataaatt cgaataaaaa tcagtataaa
     2641 cattttcgaa ataagtttta aaatcttccc atgtgttggc attgatacta ttacgtaaag
     2701 cattgattct acctgcaatt acagaagcgc tgtctttatc cactttcgac accttgctat
     2761 aaatttcctg aagttcatct ataagtttaa ccttatcttc atgcatttga gttaacagca
     2821 tagtatgtgt aattaactca cgatttttat gttcgagttc tattttcaac tgattttctt
     2881 tttccttttc gatgatcaat tcttgctcgt tgattatggc ttgttgtctt tcgagttttt
     2941 tatttacctc tttttcgaat ttatatttct tcaccataat aagtgccaat gaacttaaca
     3001 ataagatgat caatatagaa ccaataagca gatgaccttt tctggagctt tccagcattt
     3061 tgatggttaa ctgattttct aataatttct tttcggtcat tactttatcg agttcagctt
     3121 tcgaatatat ttgcaatatt ttatgggtgt tatttatatt attcagagaa tccttatata
     3181 acaaatggag ttttaaatga tcaaatgcct cactaaattt tccaatggct tgctgactct
     3241 tggccaattc atataaagcg ttagctttca atgataatct cgaagtattg aatgcctttt
     3301 cgtatgcttt ctgggcataa ccttctgctt cggtatactc gctctcatcg aaaaacaatc
     3361 gggccaatat agcataacat tcagacaata cattagggaa ttcctcataa gactcattgt
     3421 gaataacttc gtataaatag gctttagcta atgaaggttc gtccaataaa taataaattt
     3481 ctcccgaatt ttttaaggca atagccgtca tgtaaggaat ttctgcagtt ttagataaac
     3541 caatcatttt tacagaaaat tctaatgctt tcgaatattc tttcctattc ttaaagtatt
     3601 catattgttc ctggtaaatg tcgaatttta tttgtgggtc tgcaaattta ttgctggcaa
     3661 tcaaggcttt attaaaatat tcattggctt taccgaagtt gccagctttt agattcaaac
     3721 ctccaagacc tttatagatt aaagcaacat cataagattg cccgcctttt tcaattagtt
     3781 tcaaggcttt gaagtaataa tccatcgagc gttcatattc atctagctcc tgaaatatga
     3841 tggccatcaa agaatatatc ttggaacgac ctacctgata attcatcact ttacttattg
     3901 cagccgaatc cgactctatg gctattaaaa gcgaatccaa aagtttatat gattctttca
     3961 acctgctttg ataaaacatg gcacgggcta tattatacga tgaacttatc ataccatatg
     4021 gatatgcaaa ttttttcgac aacgaatcgg ctttcatcga gagaaaaagc attcgttcag
     4081 gactattagt caaattttcc gatgcgagca aaagagaatc tattcttttg attattccga
     4141 catttttcga agatgcaatc tctttacttg tttctccctt accataagga aggaacatac
     4201 ccagcaaaaa aagaataaat atgagcctca acttcaacat aataaaaccg ggaaaagtac
     4261 aaaggtaact cttcccggtt catatttcat cgaagatagt gcttatgata aactaaagca
     4321 aaatgacttt cttaataact atttgatttt caatttttaa ctgacaaaaa taaattcctc
     4381 gcgataatga tgcattttta tattggaaag aatgagcacc ggctgaaata taaccatcga
     4441 aaatttcatc tactttaact ccattcatat cgaagaggtt caaactaatc attcccgatg
     4501 aatgaacaaa aatgtcgatg tttgcctggt tgtaaaaagg attgggagat atttccaaag
     4561 atattaccgt ttgtgaactt cgaggtaaat cgatacctac aattgaatct gccttaaagc
     4621 ataaggccca tgaatcgagt gaattatttt ttgtttttga accacctgct atatatctat
     4681 tatctctcat aatttcaatc gtagaaccaa aatcatcacc atttgttcca taggtcttca
     4741 tgtcgagcat atttccctgg tcatccatta agacgattat aaaatcatgc tgtccatttc
     4801 cataactgct agtattcccc gtaatcaaat attcatgatt caatgtttca actacccctt
     4861 caaatccttc attttggtta ccaccttgag ctgaaatcca ttctacttta cccttattat
     4921 ctaccttaac tgtaaatcct tgttctttat taccatagga ttgagtggat ccaactgcaa
     4981 tataaccgcc atcactcgtt ggctgtacaa aatggaatga atcatagctt gaaccgccca
     5041 ctacacttgt ccatagggta tctccattgg gatctatttt gatcaagtag ccatcacata
     5101 aaccacttcc aaaactataa gtgtaacccg ctaatatata gtttccatcc aaagtgggct
     5161 taatacaagc tccatagtca tcattcgttg aaccgccata ttctttaaac cattcatagt
     5221 taccctcatt atctgtttta aatattacga tttgattatt atttggagga cgccatccat
     5281 atgcagcaac catataaccc ccatcaaaag tttgagtaag gcaaccagct aatgtaagat
     5341 tacaaccatt actattgggt aatatagaag tccacagagt atctcctatc tcgttaactt
     5401 taatcaaacg aataatcata tcggttgtaa aagagttaga ataggtagcg att
//
LOCUS       DAOORU010000157         5432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_18113_length_5432_cov_3.793379, whole genome shotgun sequence.
ACCESSION   DAOORU010000157 DAOORU010000000
VERSION     DAOORU010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5432
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18113_length_5432_cov_3.793379"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3658)
                     /locus_tag="PLF20_08930"
     CDS             complement(<1..3658)
                     /locus_tag="PLF20_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81160.1"
                     /translation="MMKMYQRILLIATIASLISINIYGEEFVGDKLKQSKNVKASTAG
                     CTAASAYQFLDINNVRCRINTGGDMWWNLDTGAGSRGQYFIPANTTKSSLFVGSLWIG
                     GLDVNDQLKLAALRYRQIGNDYWTGPLTIDGTAAVDAAVCAEYDKFFVISRAEVDEFL
                     GWWYSTNRSEEYPGYSIPKSIMDWPAHGDPSKFQSYYLAPFYDMDGNGEYDPNQGDYP
                     YYDIPNELCHSPVQTMEGINGIVKGSILADQVLKGDKTLWWVFNDKGNIHTESSGQAI
                     GLEIRAQAFAFSTNDEINNMTFCSYEIINRSTFTLTQTYFAPNTDPDLGYAWDDYVGC
                     DVLRGMGYCYNGKAVDGTGESEAYGDQPPAVGIDFFQGPYLDPDGYDNPSFLADGILG
                     PSFNGDCSIVNFDSTVIVMTYGKPGEEKTGNFMVRSAAINGVNFGNGIVDDERFGMRR
                     FVYYNNSYGIQGDPNIAAEYYNYLRGIWIDNTKMVYGGNAHPSNPGAVGPETDFMFPG
                     DSDPCNWSTGGAPPNGGLNQNGLYWTEVLAGNAPSDRRFVQSAGPFTLEPGAVNYITF
                     GVPWARAISGGPYASVELLRVVDDKCQALFDNCFKVLDGPDAPDLTIQELDRELIIYI
                     TNKKTSNNHREGYVERDITIKTGDSLYRFEGYQIFQLKDASVSVESIHDPAKARLVFQ
                     CDIRNGVTKLVNHYPDQAIGYSVPVVEVAGGDKGISHVFRIREDAFATGDNRLVNHKQ
                     YYYLAIAYAYNEFKKYTVEPTGLDGQKKPYLAGRKNIKVYTGIPHKTVNGLVTQSNFN
                     DGPVIMRIQGQGNGGMSLELSQATIDEILSKPMASIDNTYGSPDYPIAYQPIYQPGMG
                     PLNITVIDPLNVKKGRFSLQFIRNVEQNGVLTNAAWIMKDEEGKIYRSDSTINYYYQQ
                     LFLDLGLAVHVYQVLYPGDSLSINNGFIESNLIYADSSRRYLSGVYDNNIPSSPQNWI
                     RAGTYKDRENPKYNDWNMTSSPQKPWDANEYYEKVIGGMWSPYGLCAYHDQSNEGPAY
                     NVISKNLDPLSKLASVDIVLTPDKSKWTRSVVIEMCSDPKLSEGGVKRFNIRAAPSVN
                     KEGKKADPNSPPSTNPDDPNYISAVGFSWFPGYAINLETGERLNIMFGEDSWLSGHNG
                     RDMLWNPTSTLYENNQPIFGGKHYVYVMQHTQIIIQGDSIYEFPAYDGCRYISLILQR
                     SPENIMKTAVYGSTM"
     gene            complement(3720..>5432)
                     /locus_tag="PLF20_08935"
     CDS             complement(3720..>5432)
                     /locus_tag="PLF20_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81161.1"
                     /translation="SKTFNYYDKDGNLKTVTMGEDLFDVSLFDADELLNGGNSYVAYY
                     GYDYTGKKLSSQPSFEDFLTKKNADGEFLREVPAFRPNYLATYIQDKFAFKDLIFNVG
                     LRVDRYDANQLVLKDPYSIYPTLSVKEVQKIGDQIVSHPGNMGSDYVVYVDNATNPTT
                     ITGYRNGSVWYNATGIEIADPYILDAGSGVTPYLRNPSNQTLQKEAFQDYDPQWAFMP
                     RISFSFPISDEALFFAHYDILTQRPQGENRFDITDYYFFKTRGTTLDNPNLKSPKTID
                     YELGFKQKLTNASALTLSAYYKEIRNEIQIYRYAGAYPRDYISYNNIDFGTVKGFTIS
                     YDLRRTSNVTMRASYTLQFADGTGSSPTTAAALIAARLPNLRTPMPLAWDRRHSFNVN
                     IDYRYGEGKEYNGPRISRKNKPPVELLRNTGVNFTVIGGSGTPYTRSENITSAVSGGT
                     RILKGSFYGSRLPWQFRIDAKLDKDIHIVWKKKEGEKPKESMLNIYLQMLNIFNFKNV
                     MAVYPATGNPDDDGYLAAAEWQKEINQQLNPDTYRQLYRIYINNPANYSSPRLIRLGL
                     IFNF"
BASE COUNT         1507 a         1163 c         1179 g         1583 t
ORIGIN      
        1 acatggtgct tccatatacc gctgttttca tgatgttttc agggctacgc tgcaatatca
       61 ggctgatata tctgcatccg tcgtaagcag gaaattcata gatcgaatca ccctgaatga
      121 ttatttgggt atgttgcatg acatatacat aatgtttgcc accaaaaatg ggttggttat
      181 tttcgtataa tgtcgaagta ggattccaga gcatatctct cccattatgg ccgctcagcc
      241 agctatcttc gccaaacata atgttgagtc gttcacccgt ttcgaggttg atggcataac
      301 ccgggaacca actaaatcca actgcagaaa tatagtttgg atcatcgggg ttagtactgg
      361 ggggagaatt gggatcggct ttcttgcctt ccttgttcac cgaaggtgca gctcttatgt
      421 taaaacgttt gacacctccc tccgaaagtt ttggatcgga acacatttca ataaccacag
      481 atcgggtcca tttcgatttg tctggtgtta gcacaatatc tacactggca agcttcgata
      541 agggatcgag attctttgag ataacattat atgcagggcc ttcattgctt tgatcatggt
      601 aagcgcagag gccataaggt gaccacatgc ctccgattac cttttcataa tattcattgg
      661 catcccaagg cttttgagga gaactcgtca tattccagtc gttatatttg ggattttcac
      721 gatccttgta agtcccagca cgtatccagt tttgaggact gctggggata ttgttgtcgt
      781 aaactcccga aagataccgg cgagaactgt cagcataaat caaatttgat tcgataaaac
      841 cattgttaat agagagcgaa tcgcccgggt acaaaacctg ataaacatga acggcaagtc
      901 ctaaatcgag gaaaagctgt tgatagtaat aattgatcgt gctatctgat ctataaatct
      961 tcccttcttc atctttcatg atccaggctg cgtttgtcag cactccattc tgttcgacgt
     1021 ttcttatgaa ttgcaggctg aatctacctt tttttacatt caagggatcg attacggtga
     1081 tgttgagagg acccatgcct ggctgatata taggttgata ggcgatggga taatcgggac
     1141 tgccataagt attatcaatg gaagccatag gtttggacaa aatctcgtct attgtggctt
     1201 gcgaaagttc caatgacatt ccaccatttc cttgaccctg aattctcata ataactgggc
     1261 catcattaaa attagattga gtaaccaatc cattgactgt cttgtgagga atacccgtat
     1321 aaacctttat attcttacgt ccagccagat atggtttttt ctgaccatct aatcctgtag
     1381 gttctaccgt atatttctta aattcattat aagcataggc aatggctaaa tagtaatatt
     1441 gtttgtgatt gaccagacga ttatctccgg tagcaaacgc atcttccctt attctgaata
     1501 catgactgat acccttatca ccgccagcaa cttcaacaac gggtacgcta tagcctatag
     1561 cctgatcggg atagtggttg accagtttgg taacgccatt acgaatatcg cattgaaata
     1621 ccagtcttgc cttagcagga tcgtgtatgc tttcgaccga aactgaggca tccttcaatt
     1681 gaaatatctg gtatccttcg aaacggtaga gcgaatcacc tgttttaatg gtgatatcgc
     1741 gttcgacata accttcacga tgattgtttg aagttttctt attggtgata taaataataa
     1801 gttcgcggtc gagttcctga atagtcaaat cgggagcatc ggggccatcg agaactttaa
     1861 agcaattgtc gaaaagagcc tgacacttgt catcaactac ccgcaggagt tcaacggagg
     1921 cataaggtcc tccggaaatg gctctagccc atggtacacc aaaagtgata taattcacgg
     1981 ctccgggttc aagagtaaat ggacctgctg attgaacaaa acgacggtcg gaaggtgcat
     2041 ttccagcaag aacttctgtc cagtatagcc cattctggtt aagaccgccg ttaggaggtg
     2101 caccaccagt tgaccagtta cagggatcgg aatcaccggg aaacatgaaa tcggtttcgg
     2161 ggccaacggc tccgggattg ctggggtgtg catttccgcc ataaaccatt tttgtattgt
     2221 ctatccaaat tccacgtagg taattataat attcagcggc tatatttggg tcgccctgaa
     2281 taccatagga gttattataa taaacgaacc tgcgcattcc aaagcgttcg tcgtcaacga
     2341 ttccattccc aaaattcact ccattgatgg ctgcacttct caccatgaaa ttccccgttt
     2401 tttcttcacc gggtttacca taagtcatta caataaccgt agaatcgaag ttcacgatgg
     2461 agcaatctcc gttaaatgaa ggaccaagaa tgccatccgc caagaaagaa ggattatcat
     2521 acccatcagg gtcaaggtaa ggcccttgga aaaagtctat ccccacggca ggaggttgat
     2581 caccataggc ttctgattca cctgtcccgt caacggcctt tccattatag caatatccca
     2641 tgcctctcaa tacatcgcat cctacataat catcccatgc ataacctaaa tcagggtcag
     2701 tattaggagc gaaataggtt tgggttaagg taaaagtgga acggttgata atttcataac
     2761 tgcagaaagt catgttgttg atttcgtcgt ttgtggagaa agcaaaagcc tgtgcacgaa
     2821 tttcaagacc aatagcctgt ccgctcgatt cggtatgtat atttccttta tcattaaata
     2881 cccaccatag ggttttatct ccttttaaaa cctggtcggc caagatactc ccttttacga
     2941 tgccattgat cccttccatg gtttgtacgg ggctatggca aagttcattg ggaatatcat
     3001 aatagggata gtctccttgg ttggggtcgt actctccatt cccatccata tcgtaaaatg
     3061 gagcgaggta ataactctga aacttcgatg ggtcgccatg agctggccaa tccatgatgg
     3121 atttgggaat gctgtaaccg ggatattctt cggaacgatt cgtactgtac caccatccga
     3181 ggaattcatc tacttcggca cggctgatga cgaaaaactt gtcatattca gcacagacgg
     3241 cagcatccac agccgctgtg ccatcaatgg taagaggacc agtccaataa tcgttcccaa
     3301 tttgtcgata acgcaaagca gcaagcttta actgatcgtt gacgtcgagg ccaccaatcc
     3361 acaaagagcc gacaaaaaga gaagacttag tggtattggc aggtataaaa tattgaccac
     3421 ggcttccagc acctgtatcg agattccacc acatatctcc tcctgtatta atgcggcagc
     3481 gcacattatt gatatcgagg aattggtaag cagaagctgc ggtacatcct gctgtgcttg
     3541 cctttacatt tttcgattgt tttaacttgt cgcccacaaa ttcttcaccg tagatattta
     3601 tactaataag cgaagctatc gtggcgatta gtagaatgcg ttgatacatt ttcatcatat
     3661 tattcgtttt gtttgttatc atatcaaaac ttatacatta tctttcaaaa aatggaagat
     3721 taaaaattga atatcaagcc cagacgaatc aatcgtggac tgctgtaatt agcaggatta
     3781 ttaatataaa tacgatagag ctggcgatag gtatcgggat tgagctgctg attgatttcc
     3841 ttctgccatt cggcggcagc aagataacca tcatcgtcgg ggttgcctgt agctggataa
     3901 acggccatca cattcttgaa attgaaaata ttgagcattt gcaaataaat attcagcatg
     3961 gattccttag gcttttctcc ttctttcttt ttccagacta tgtggatatc tttatcgagc
     4021 ttagcgtcga ttcggaactg ccatggcagt cttgagccat agaagcttcc ttttaagata
     4081 cgtgtaccac cgcttactgc cgaagtaatg ttttccgaac gggtgtaagg cgttcccgaa
     4141 ccaccaatta ccgtgaaatt aacgcctgta tttctgagga gttcgacagg aggcttgttt
     4201 ttccggctga tacgaggccc attgtattct ttcccttctc catagcgata atcgatattg
     4261 acattgaagc tatggcgtct atcccatgca aggggcatag gtgtacgaag gtttggcagg
     4321 cgtgcagcta tgagtgcagc ggcagtggtt gggctcgaac ccgtaccatc ggcaaactgc
     4381 aaggtatagg aagcacgcat ggttacattg ctcgtacgtc tcaaatcgta actaatggta
     4441 aatcctttta cagtaccaaa gtcaatattg ttataggata tataatctct gggataagct
     4501 cccgcgtaac gataaatctg tatttcattc cggatttctt tataatatgc cgatagggta
     4561 agggctgaag catttgtcaa tttttgctta aaacctaatt catagtcaat ggttttggga
     4621 gatttcagat tcggattatc gagggtagtt ccacgggttt taaagaagta ataatcggtg
     4681 atatcgaatc ggttttctcc ctgaggacgc tgggtaagga tgtcataatg ggcaaagaac
     4741 aaagcctcgt cggaaatagg gaatgagaaa gagattcgag gcatgaaggc ccattgcggg
     4801 tcataatctt ggaatgcctc cttttgtaag gtttgattgg acggatttcg taagtaggga
     4861 gtaactccag aaccggcatc cagaatgtaa gggtcggcta tttcgattcc agtggcatta
     4921 taccatacac tgccattacg atagcctgta atggtggtgg gattggtggc attatccaca
     4981 taaaccacat aatcagagcc catatttcct ggatgactaa ctatttggtc tcctattttt
     5041 tgaacctctt tcaccgaaag ggttggatat atcgagtaag ggtcttttaa aacgagctga
     5101 ttggcatcgt agcggtccac acgtaagccc acattgaata tcaggtcctt aaaagcgaat
     5161 ttatcctgaa tgtaggtagc caagtaattg ggacgaaaag caggaacttc acgtaggaac
     5221 tccccatcgg cattcttctt cgtaaggaaa tcttcgaatg atggttgact ggagagtttc
     5281 ttacccgtat aatcatatcc atagtaagct acatacgaat tgcctccatt taatagctca
     5341 tcggcgtcga agagactaac atcgaataga tcctcaccca tggtaacggt tttcaagttt
     5401 ccatccttat cgtaataatt gaaggttttc ga
//
LOCUS       DAOORU010000158         5392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_18270_length_5392_cov_4.501218, whole genome shotgun sequence.
ACCESSION   DAOORU010000158 DAOORU010000000
VERSION     DAOORU010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18270_length_5392_cov_4.501218"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="PLF20_08940"
     CDS             complement(<1..640)
                     /locus_tag="PLF20_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HOX81162.1"
                     /translation="MDPLVYIILAFVFFPVFLLSIFKDVGIAPWKTLIPFYNYYLWNK
                     MIGKRLYWFLLLFVPFINVFMVFLMEVEIAKCYQKYDLGHQALAVLFPVIYLPYLGLS
                     AKEKYLEPSKRPVIKKTALREWVDALIFAVVAALIIRTFIFEAYTIPTPSMEKTLLVG
                     DYLFVSKMSYGPKVPNTPLSFPFVHHTLPFTQYSKSYVEWIKLPYYRFPGFGK"
     gene            813..2741
                     /locus_tag="PLF20_08945"
     CDS             813..2741
                     /locus_tag="PLF20_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOX81163.1"
                     /translation="MVSNDKYDIQNILSQYFGYKSFRPLQEDIIRSVLWRKDTLALLP
                     TGGGKSICYQIPALAMEGVCLVVSPLIALMKDQVETLKKKGVEAEAVFSGMSSYQIEK
                     ILDDAAYGNLKFLYLSPERLITSSFRDRLPYMKLNLIAVDEAHCISQWGYDFRPPYLR
                     IAEIREFHQETPILALTATATSMVINDIQEKLQFKEKNVLSRSFYRPNLIYVVQKEGD
                     KARRLLKICSNLKGSGIVYVRNRRRTVEIAKFLVRNHHSAGVYHAGMSMKQRNNVQNR
                     WMNDELKVMVATNAFGMGIDKPNVRFVVHMDIPDSPEAYFQEAGRAGRDGKLSYAILL
                     YDESDMSDASENFNNSWPSYGVIKNVYNALGNYYNLAIGSGKDISFDFDLNDFCNRFN
                     LKPLPTFNALKIIEKEGYIALNDALDSPSQLMFRLSGDGMYNFQVANKKFDGFIKIIS
                     RMYGGALYTSHQRIIEEAIAKYIHITTDQVIQLLHELDHLEVVSYMPRKNKPQLVFVI
                     ERLDLSNFYLSPENYRLRKEAAQKRLEAMLHYIKDENHCRSQILLSYFGEKTRMRCGA
                     CDYCVKHHSLPLGEKEFEQIVEELKKIIKDQSISLPEVLRQMSPDSDEDNVMQVIVYL
                     TENGKLVRDSFGLLSFKQ"
     gene            complement(2794..3066)
                     /locus_tag="PLF20_08950"
     CDS             complement(2794..3066)
                     /locus_tag="PLF20_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOX81164.1"
                     /translation="MNKAELIDAIATNSGLTKAASKKALDATILAISEALKQGDKVSL
                     IGFGTFGVAKRSARTGRNPRTKAPLQIPAKNVVKFKPGSELSKSVK"
     gene            complement(3401..4375)
                     /gene="fmt"
                     /locus_tag="PLF20_08955"
     CDS             complement(3401..4375)
                     /gene="fmt"
                     /locus_tag="PLF20_08955"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOX81165.1"
                     /translation="MEKNLKLIYMGTPEFAVAPLKALITNGYRVEAVVTAPDKPAGRG
                     QRMQSSPVKSYAQSVGLPLFQPYFLKDPQFIAQLENLTPDIILVVAFRLIPPEIWKLT
                     RIGAINLHASLLPDYRGAAPIQRAIMNGETITGITTFFIDEGIDKGKIILQEVIPIGE
                     TETAGSLHFKMMQQGAELVLKTLDTIARGNYQLKDQGKLICEKKLNLHYAPKISKEDC
                     KIIWHNSAKNIFNQIRALNPHPGADTLLMAPDQTAQILKVYYATPIFEPLGTITPGSI
                     NTDGKSFLQVACGDGFIELTEIQLSGKKRMDIASFLRGFKLDNRWTLI"
     gene            complement(4446..5138)
                     /locus_tag="PLF20_08960"
     CDS             complement(4446..5138)
                     /locus_tag="PLF20_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOX81166.1"
                     /translation="MSKKKKKKEVTNGRIEAVESALSKSERFLEENQKVILIVVSAII
                     VIVLGFLGYQRLIIAPKEVKAANEIFMAQNYFEHDSLYKALNGDGQYAGFLEIVDRYG
                     ATSTGNLAKYYTGICYLKLQDFDNAIKYLKKFNTKNPVAKSMALGGIGDAYLELGKNE
                     KAIEYYKKAAQQKNEFTSPLFLMKAGLSYELLNDYQNALKVYEEIRMNYPKSYEARDI
                     DRFIAKAKGMLR"
BASE COUNT         1685 a          996 c         1048 g         1663 t
ORIGIN      
        1 ctttaccaaa ccctggaaat cgataatagg gtaacttaat ccactcgaca tacgattttg
       61 aatattgagt gaaaggaagt gtgtgatgaa cgaaggggaa cgataagggt gtattgggta
      121 ctttaggccc ataagacatt ttgcttacaa aaaggtaatc gccaacaaga agcgtttttt
      181 ccatcgatgg agtagggatg gtgtacgcct cgaaaataaa cgtacgaata atgagagcag
      241 ctactactgc aaaaattaaa gcgtcgaccc attctctcaa agcagttttt tttatcacag
      301 gccgtttcga aggctcgaga tatttttcct tggccgataa tcccaaataa ggtagatata
      361 ttacaggaaa caagactgcc aatgcttgat gacccagatc atatttctga taacacttgg
      421 caatctccac ctccatcagg aaaaccatga aaacattgat gaaaggaaca aacaacaata
      481 aaaaccaata aagtcgtttc cctatcatct tattccataa ataataatta taaaaaggga
      541 taagtgtttt ccatggtgct ataccaacat ccttaaatat tgacaataaa aaaaccggga
      601 aaaagacaaa ggctaaaatg atgtatacca aagggtccat atattcttta tgtattgata
      661 ttcagtttta acgatacatg tgtaaatttg ttgcaatgat agtaattttg aaggaattcg
      721 attcctgact ttatgttctc cttagtagag gaacaaactc agaatgtgta tgaattttat
      781 gtatcaatat acttttgata tagagtaaaa aaatggttag taacgataaa tacgacatac
      841 aaaatatcct ttcccaatat tttgggtaca aatcgttcag accattgcag gaagacatta
      901 ttcggtcggt attgtggagg aaagatacac ttgcgttatt accgacagga ggagggaaga
      961 gtatttgtta tcaaattcct gccttagcca tggaaggggt ttgcttagtg gtttcgcctc
     1021 tgatagcctt gatgaaagat caggttgaaa ctctcaaaaa aaaaggagtc gaagccgaag
     1081 ctgttttttc gggcatgagt tcatatcaga tcgaaaaaat tctcgacgac gcagcttatg
     1141 gcaatttgaa attcttatat ttatcacccg aaaggcttat cacttcctca tttcgagacc
     1201 gattgcccta tatgaaatta aatctgatag ccgtcgacga agctcactgc atttcgcaat
     1261 ggggttacga cttcaggcct ccatatcttc gtattgctga aatcagagaa ttccatcaag
     1321 aaacccccat acttgccctt actgcaacag ccacttcgat ggtcatcaac gacatacagg
     1381 agaagctgca gtttaaggaa aaaaatgtat tgagtcgtag cttttatcgt cccaatctaa
     1441 tatatgtggt acaaaaggaa ggtgataagg cgagacgttt attgaaaatc tgcagcaatt
     1501 taaaaggctc ggggatcgtt tacgttcgga acagacgcag aaccgttgaa atagcaaaat
     1561 tcctggttcg gaaccatcat tcggccggcg tctatcatgc agggatgtcc atgaagcaaa
     1621 ggaataatgt acaaaaccgc tggatgaacg atgaattaaa agtaatggta gcaaccaatg
     1681 catttggtat gggaatagac aaacccaatg taaggtttgt cgttcacatg gatattcccg
     1741 attctcccga agcatatttt caggaagcag ggcgcgccgg aagagatgga aaattatcgt
     1801 atgccatatt gctttatgat gaatccgata tgagtgatgc cagcgaaaat tttaataatt
     1861 catggccaag ctatggagta attaaaaatg tttacaatgc tctgggaaac tattataacc
     1921 tcgccattgg atctggaaaa gatatctcgt tcgatttcga cttgaatgat ttttgcaatc
     1981 gattcaatct aaagccatta cctactttca acgcattaaa aatcatcgaa aaagaaggtt
     2041 atatagcttt gaatgatgcc ttggactctc cctcccagct tatgtttcga ctttcgggag
     2101 atggaatgta caattttcag gtagccaaca agaaattcga tggtttcata aagattattt
     2161 cacgcatgta tggaggggct ctatacacca gccatcagcg tatcatcgaa gaagccattg
     2221 caaaatacat ccatataaca actgaccagg tgattcagct tttgcatgaa ctcgatcacc
     2281 tcgaagtggt ctcttatatg cctcgcaaaa ataagcccca gctcgttttt gttatagaaa
     2341 gattagacct ttccaatttt tacctatcgc cagaaaatta tcgtttaagg aaagaagctg
     2401 ctcaaaaaag gctcgaagcc atgctccatt atataaaaga cgaaaatcat tgccgaagcc
     2461 aaattctttt atcttatttt ggagaaaaaa ccagaatgcg ttgtggggca tgtgattatt
     2521 gcgtaaaaca ccattcactt cccttgggcg agaaagaatt cgaacaaatt gtagaagaat
     2581 tgaaaaaaat tataaaagat cagtcgatta gtttacccga agttcttcgg caaatgtcgc
     2641 cagattcaga tgaagacaac gtgatgcagg ttattgttta tttgactgag aatggaaaat
     2701 tggttcgcga cagttttggt ttgttgagct tcaaacaata agaaagcccc tcgattagcc
     2761 atcatggggg ctttcttatc aaaaaatttc tacttactta acactttttg aaagttcact
     2821 gccgggttta aatttcacaa catttttggc agggatttga aggggagctt tggttcgagg
     2881 attgcgtcct gtgcgggcac ttcttttggc tacaccaaaa gttccaaaac caatcagaga
     2941 gactttgtcg ccttgtttca aagcttcaga aatagctaag atggtggcat cgagggcttt
     3001 cttcgaggct gctttggtaa gtccggaatt ggtagcaatg gcatcgatta attctgcttt
     3061 gttcataatt gtaggttttt aggttaacat ttaatggtag tgcaaatata tatataaact
     3121 ctttgaaatc aagagaagca ttcattttta cgcaaaattg ttgaaaaaaa tcaaattttg
     3181 ttaataaatt ccctcttttc ggctgcttct aaaccctgtt cactctcatt gagattcaca
     3241 aaaaacatag gttgaatttt tgatgaattt ttttattaaa gcaggaaaaa tgaaagaaat
     3301 ttattataaa taattatttt acagtatatt aaatatgaaa taattggcaa gcgtttagat
     3361 ttttttctaa aacttccaac cccaagacag atgagatact ttaaatcaga gtccagcggt
     3421 tatcgagttt aaatcctcgt aaaaacgagg caatatccat tcttttttta ccacttaact
     3481 gtatctcggt caattcgatg aaaccatcgc cacacgcaac ttgtaagaag gatttaccat
     3541 cggtattgat gcttccggga gttatggtac caagaggttc aaagatgggg gttgcataat
     3601 agactttgag gatttgggca gtttggtcag gtgccattag cagtgtatct gctcctggat
     3661 gaggattcaa ggctctgatt tgattaaaaa tatttttagc cgaattgtgc cagataattt
     3721 tacaatcttc tttgctaatt ttcggagcat aatgcaggtt caatttcttt tcgcagatga
     3781 gtttgccttg atctttaagc tgataatttc ctctggcaat ggtatcgaga gttttcagaa
     3841 caagttcggc tccttgttgc atcattttga agtgcaggct gccagcggtt tctgtttctc
     3901 caatgggaat aacttcctga aggatgattt ttcccttgtc tattccttcg tctataaaaa
     3961 aagtggtaat acccgttatg gtttctccat tcattatggc tctttgtata ggtgccgcac
     4021 ctcgatagtc gggcagaaga gatgcatgaa gattgatagc tcctatccgg gttaatttcc
     4081 atatttcggg tggaattaat cgaaaagcca cgaccagaat aatatcagga gtcaaattct
     4141 cgagctgtgc tataaattgt ggatctttca agaaataggg ttgaaacaag ggcaagccaa
     4201 ccgattgtgc ataagatttt acggggctcg actgcatacg ttgacctctt cctgctggtt
     4261 tatcaggggc cgtaacaaca gcttcgactc tataaccatt tgtgattagg gcctttaacg
     4321 gagccacagc aaattcgggc gtccccatgt aaatcagttt caaatttttc tccatctacc
     4381 aggtataaaa atgaaaaaag cctgatcata atttttgaat caggctcaat atgaattata
     4441 aaaaattatc tgagcatacc tttggctttg gcaataaaac ggtctatatc acgtgcttca
     4501 tagctttttg gatagttcat ccgaatttct tcataaactt tcagtgcatt ctgataatca
     4561 ttcaggagtt cataactcaa acctgccttc ataaggaaga gaggagaggt aaattcattt
     4621 ttttgttgag cggctttttt gtaatattct atcgcctttt cattcttgcc taattcgagg
     4681 taagcatcgc ctattccacc gagagccatt gatttagcca caggattttt cgtattaaac
     4741 tttttgaggt atttgatggc gttgtcaaaa tcctgaagtt ttaaataaca aatgccagta
     4801 taatattttg caaggttgcc tgtgctggta gcgccatatc tgtcgactat ttcgaggaaa
     4861 ccggcatatt gtccatcacc attcagtgct ttatacaaag aatcatgttc aaaataattc
     4921 tgggccatga agatttcgtt ggctgcctta acttctttgg gagctatgat caatctttga
     4981 tagccaagaa atcctaatac aattactatt attgcactta caactataag aataactttc
     5041 tgattctcct ctaaaaagcg ttcacttttg gaaagggcgc tttcaacggc ttcgattctt
     5101 ccgttggtta cttctttctt tttctttttt ttactcattc tgtttgctaa attttttggg
     5161 catgcaaaat taaaaaatga ttgataaatt ccataattcc ttcaattaaa attgataaat
     5221 caaaggctat cccctgataa taaaatacaa tacactcttt ttcattatat tatgaattta
     5281 ttctatggat atactccctt tattgattag ccaaagaaaa gataggccat gaaaattgca
     5341 gtaataatcc cagccagatc ggcaataagc ccgcaaacaa cggcataacg gc
//
LOCUS       DAOORU010000159         5311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_18623_length_5311_cov_4.351218, whole genome shotgun sequence.
ACCESSION   DAOORU010000159 DAOORU010000000
VERSION     DAOORU010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18623_length_5311_cov_4.351218"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1731)
                     /locus_tag="PLF20_08965"
     CDS             complement(<1..1731)
                     /locus_tag="PLF20_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81167.1"
                     /translation="MHNNKSFFCYLHFLLGKAIHQFPILWILWLAFFISNPLKINCQN
                     NQSEELPAIEEIPVSIRIESVGAGMMNSLYEYESEKLFLPVLELFRFLQIKAEPSVTL
                     DSISGFIGKEEYRYLIDNHYKKIFFRGKDFSIAENQSMNTGDDLYIDSRLFGEIFGLH
                     CKFNFRNLSVTIKPDFELPVIRKMRIEQFRKNIKQLRGEVEADTTLKHQHELWKFGMM
                     DWAATSTQSTDSKTSSQFWLRTGAEALYGETNLQLYYSSLNEFTNRNLQYYWRWVDNQ
                     NKVIRQVRAGRISPMSISSIYNPITGISITNASTLYRRSFGEYTISDYTEPGWTVELY
                     VNNVIVDYQIADASGFYKFNIPLVYGASEVMLKFYGPYGEERIQRIFLNVPFSFLPPG
                     ELEYTVNSGLVLDDKNSHFERADVKYGVKRYLTIGGGFEYLSSIVSGKEIPFINASVA
                     PVRNLLISSEYAKGVQTKLLANYRLVSGSSFELKYTHYVPGQQAVRVSYLEDWQATLS
                     MPVRFPKFSAYSRLSYRRNVYKTTTYNIASITLSSYIKKLNASISAYANWMDGDDPFI
                     YANIGLGLPQW"
     gene            complement(1738..2226)
                     /locus_tag="PLF20_08970"
     CDS             complement(1738..2226)
                     /locus_tag="PLF20_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOX81168.1"
                     /translation="MRRVKIFIGLIFFSFSLYAQRTSELKVHVSQELSFGSFYSGRSG
                     GTITISPDGNRWVTGEIILLVNSSGCPALLEIEAPPNRTIHVEFPVSAEIKQAGGQQN
                     LTINNFTTDKLNNSLLTSSRDLPYGNQVRVGATLTVPGSVTLSSGNYIGNFRINVVCI
                     QE"
     gene            complement(2321..3202)
                     /locus_tag="PLF20_08975"
     CDS             complement(2321..3202)
                     /locus_tag="PLF20_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HOX81169.1"
                     /translation="MHKGKLIKTRLQHRGRAYGVKGGWFFVILLLVCPFLGIAQGDLL
                     ITPRRVVLEGNKKTEEIAVANVGQDTAFYNISLIEFHMHEDGRMEQITEPMSGQKFAT
                     PMLRFFPRSIKLAPQESQVIRIQVRRLPNMEEGEYRSHLYFRAVPKSRPEGEKTEETD
                     TTSLKIRLIPIYGISIPVIVRIGNLSATLTISDLSLEQQEGQTTRLKMTLNRQGTQSV
                     YGNVTVDYVAPGKEPVRVSLVKGVAVYTPNTLRYFSMPLTLPEGFSWDKGKLVIRFTD
                     ANEAKPYIFDEKELVLP"
     gene            complement(3267..3785)
                     /locus_tag="PLF20_08980"
     CDS             complement(3267..3785)
                     /locus_tag="PLF20_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HOX81170.1"
                     /translation="MKKLGLFTAGLFVMVFAAQNVNAQSGAQASASANATATVVSVVT
                     VAKSADLDFGTFASGLTAGTVSMETNGDRYKTGGVVLLPTDVGNPATFTAYGPATEHY
                     FITLPDDDDVTLTLSGGTETMDISGFIHNASGIFGAGGSESFNVGGVLNVHALQAAGL
                     YTGTFDVIVTLQ"
     gene            4066..4443
                     /locus_tag="PLF20_08985"
     CDS             4066..4443
                     /locus_tag="PLF20_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81171.1"
                     /translation="MNAIESLFPIHEANAIIMDEHRAFCPKNSYLVLIHSCFLGLIPF
                     FEAIYQKCGLHNTAGEAKCMLQHWIPSLELIRLRNFPYHYRFDISTAKFHPFVLLFIL
                     RMIIFAFSLDLKILKGLCHEDLR"
     gene            4427..>5311
                     /locus_tag="PLF20_08990"
     CDS             4427..>5311
                     /locus_tag="PLF20_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOX81172.1"
                     /translation="MKIYDNEIEKLKQISQLQVDFVKHLLEVINHPDIFLHLSRELHK
                     LVKFDIFNFAILNASLDVIIEATFIKLEDDSFRFIHLPDSFKILPPKREYFSKGSPSL
                     FRLSSEKFLALIGSDSLYREIRNLNIRSVVFMALNPEESRKMILSLGSKSADAFSEEE
                     TMVLECLLPQIRLIIENYFSFEQCQQLRAQLENEKKASLKEWQFKFGKEDLVAESQQM
                     KQVLYKVRQVAPTDSTVLLEGETGVGKELIAKAIHCSSSRNKGPLVMVNCAALPPNLI
                     ESELFGHEKESFTGASERR"
BASE COUNT         1543 a         1074 c         1050 g         1644 t
ORIGIN      
        1 ccattgaggc aaaccgagtc cgatatttgc ataaatgaat ggatcgtctc catccatcca
       61 attggcatac gcacttatac tggcattcag tttttttata tatgacgaaa gtgtaatact
      121 ggcaatgttg taagtagttg ttttatatac atttcgccta tacgatagac gagaataagc
      181 actgaatttt gggaaacgaa ccggcatcga aagagtggct tgccaatcct ccagatagct
      241 tacccgcacg gcttgctgcc cgggaacata atgagtatat tttagctcga acgatgaacc
      301 cgatacaagc ctgtaatttg ccaacaattt ggtttgtact cctttggcat attcactgct
      361 aatgagtaaa ttccggactg gcgcaacgga tgcatttata aaaggtattt ccttgcccga
      421 aacgatagaa gacagatatt cgaaaccacc cccaatggtc agataacgtt tgacgccata
      481 ttttacatcg gctctttcaa aatgtgagtt cttgtcatcg agaacaagac cagaattcac
      541 agtatattcc agctcacctg gcggtaggaa gctaaaagga acatttagga atatacgctg
      601 aatgcgttct tcaccataag gcccataaaa tttcagcatc acctccgaag ctccatatac
      661 caaaggaata ttgaatttgt aaaaccctga agcatcggca atttgataat ctacaatcac
      721 attgtttacg taaagttcga cagtccagcc tggctcggtg tagtcggaaa tagtgtattc
      781 tccaaaagaa cgacgatata aggtcgaagc attggtaatg ctgatgcctg ttataggatt
      841 atagatggaa gaaatagaca taggagatat tctgcctgct cttacctggc gaatgacttt
      901 attctggttg tccacccatc gccagtagta ttgtaagttg cggttcgtaa attcattaag
      961 cgaggaataa taaagctgca aattggtttc gccatataat gcttcggcac cggttcttaa
     1021 ccaaaactgg ctgctggttt tgctgtcggt gctttgtgtc gaagtggcag cccaatccat
     1081 cataccgaat ttccaaagct catgctggtg ctttaaagta gtatctgctt ctacctcacc
     1141 tcttaactgt ttgatgttct ttctgaattg ttcgatcctc attttcctta tgacaggcaa
     1201 ttcgaaatcg ggttttatcg taacagaaag attgcgaaag ttgaacttgc aatgcaaacc
     1261 gaatatttca ccaaaaagac gagaatctat gtaaagatcg tctcccgtat tcatcgactg
     1321 attttcagct atggaaaaat ctttccccct gaaaaagatc tttttgtaat ggttatcaat
     1381 aagatagcgg tactcctctt ttcctatgaa accgctgata gaatcgagtg taaccgaagg
     1441 ttctgccttt atctgaagaa atcggaataa ttctaatacg ggcaaaaaca gcttctccga
     1501 ttcatactca tagagggaat tcatcatacc tgcccctaca ctttctatcc gtatgctgac
     1561 aggaatttct tctatcgctg gtagttcttc gctttgattg ttttgacaat ttattttgag
     1621 aggattcgaa ataaaaaatg ctagccataa aatccacagg ataggaaatt gatgtatggc
     1681 ttttcccaat aagaaatgaa ggtagcaaaa gaaggatttg ttgttatgca cattaatcta
     1741 ttcctgaata catactacat ttattctgaa attgccaata taatttccgg aagaaagggt
     1801 gactgagcct ggtactgtaa gcgttgcccc gaccctaacc tgatttccgt aaggaaggtc
     1861 tctggaggaa gttaataaac tattgttgag tttatcggtg gtaaaattat tgatagtaag
     1921 gttttgctga ccccctgcct gctttatttc tgcagaaacg ggaaattcaa catgaatggt
     1981 acggttagga ggtgcttcaa tttctaataa agcaggacat ccactcgaat tgactaataa
     2041 gataatttca cccgtgaccc atcgattgcc atctggagaa atagtaatag ttccaccact
     2101 acgtccacta taaaatgaac cgaatgataa ttcttgactt acatgaacct ttaattcgct
     2161 ggtcctttgg gcataaagag aaaatgaaaa aaaaataaga ccaatgaaaa tctttactct
     2221 ccgcatatcc ttgggcattt tattcttttt cttctcatca tcttatttga ttatttttat
     2281 atttacctct acgatacaaa aatttatcaa gtgaagtttt tcaaggcaat accaattctt
     2341 tttcgtcgaa gatataaggt tttgcttcgt ttgcatcggt aaaacgtatt acaagttttc
     2401 ctttatccca actaaaccct tcgggaagtg tcaaaggcat cgaaaaatag cgtaaagtat
     2461 tgggcgtgta aacggcaacc ccttttacca ggcttactct tacgggttct tttccgggag
     2521 caacataatc taccgtaaca ttgccataaa ccgattgcgt gccctggcgg ttcaatgtca
     2581 tcttaagccg cgtggtttgc ccttcctgct gctcgaggct taaatcactg atggttaaag
     2641 tagccgacaa attaccgatg cgtacgataa ctggaatgga gatgccatag atagggatga
     2701 gccttatttt gagagaagta gtgtcggttt cttcggtttt ttcgccttct ggcctcgatt
     2761 tgggtacagc cctgaaataa aggtgcgaac gatattcgcc ttcttccata ttgggaagcc
     2821 gccttacctg tattcgtata acttgtgatt cctgaggcgc aagcttgatg gaacgcggga
     2881 aaaaacgcaa cattggcgta gcaaacttct gcccggacat gggttcggta atctgttcca
     2941 tacgcccatc ttcatgcatg tggaattcta tcagtgaaat attatagaat gccgtatctt
     3001 gccctacatt ggctaccgct atctcttctg ttttcttgtt tccttccaac accacccgcc
     3061 gtggagtaat gagcaaatcg ccctgcgcta tccctaaaaa tggacacact aaaagtaaaa
     3121 taacgaaaaa ccatccccct ttaacaccat aagcccttcc cctgtgttgc agtcttgttt
     3181 ttataagttt tcccttgtgc attcgttcgt ttttaaaaat taaaagggca gctttaggcc
     3241 gcccctttac taagaagttg atttcattat tgcagggtaa cgattacatc gaaagttccg
     3301 gtatatagtc ctgcagcttg aagtgcatga acatttaata ctcctcctac attgaatgat
     3361 tcagaaccgc cggcaccaaa aattccagaa gcattatgaa taaatccact aatgtccatc
     3421 gtctcagtac cacctgataa agtaagggta acatcatcat cgtcaggtaa agttataaaa
     3481 taatgttcag tagctggtcc atacgcagta aaggttgcag ggttacccac atccgtaggt
     3541 aagaggacaa cacctcctgt tttatatctg tccccatttg tttccattga tacggtaccc
     3601 gctgttaatc cactggcaaa ggtaccaaaa tcaaggtctg ctgatttggc aacggtgaca
     3661 accgagacca cagttgctgt tgcattagcg cttgcacttg cctgtgcacc tgattgggcg
     3721 ttcacatttt gagcggcaaa gaccattacg aaaaggcctg cagtaaataa tcctaacttt
     3781 ttcatacact tgggtttttt taaatttatg aattggtttt taaggttttt tcccgcatct
     3841 tcggacaact ttttttggaa aaattcatgt cgtaaagacg aaaggatttt tgttttttaa
     3901 attgactgtg aagaatagca aaattaaagc catagtgcca aaaatttttt tccttttgtt
     3961 ttatattgat tttcaatgga atataatttt ccccgaaata ttatttttct taatattaag
     4021 aatttcctat ttttgcattc ttaaatttca gaaattcccc tttttatgaa tgccatagag
     4081 agtttatttc ctattcatga agccaatgcc attattatgg atgaacatcg agctttttgc
     4141 ccgaaaaaca gttatttggt tctaattcac tcttgctttc tcggattaat accatttttc
     4201 gaagccattt accagaagtg cggtcttcat aatacggccg gcgaagccaa gtgtatgctt
     4261 caacattgga ttccgtccct tgaacttatc aggcttcgga attttcctta tcattatcga
     4321 ttcgacattt ccacagccaa gtttcatccc tttgtattgc tttttatctt acgaatgatc
     4381 atctttgctt tttcgttgga tttgaaaatc ttgaaagggc tttgtcatga agatttacga
     4441 taatgaaatc gaaaaattga agcagataag ccagcttcag gttgactttg taaaacatct
     4501 tctcgaggta ataaaccatc ctgatatctt cctgcatttg agcagggaat tacacaagct
     4561 ggtaaaattc gatattttta attttgccat tctcaatgcc agcttagatg ttattatcga
     4621 agcaactttt ataaaattgg aagatgattc ttttcggttt atacatttac ctgacagctt
     4681 caaaatttta ccacccaaaa gggaatattt tagcaagggg agcccgtctc tatttcgcct
     4741 gagcagtgaa aaatttctgg cactcattgg ttcggacagt ctttatcggg aaatccgcaa
     4801 cttaaatatt cgatcggtag tttttatggc tttgaacccg gaagaatcgc gtaagatgat
     4861 attatcatta ggcagcaaat cggccgatgc atttagcgaa gaagaaacaa tggtgttgga
     4921 atgtttatta cctcaaataa gattgattat tgagaattat ttttcgttcg agcaatgtca
     4981 gcaattaaga gctcagttag agaacgaaaa gaaagcctca ttaaaggaat ggcaatttaa
     5041 gtttggcaaa gaggacttag tggcggagag ccagcaaatg aagcaagtat tgtataaggt
     5101 caggcaggta gctccaacgg attccacggt attattggaa ggggaaaccg gtgtggggaa
     5161 agagcttatt gcaaaagcca tacattgcag ttcctcacgg aataagggtc cgttggtaat
     5221 ggtgaattgt gcggctttgc cacccaacct tatcgaatcg gagctttttg gacatgagaa
     5281 ggagagtttt acgggagcct ccgagcgtcg g
//
LOCUS       DAOORU010000160         5292 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_18750_length_5292_cov_4.588314, whole genome shotgun sequence.
ACCESSION   DAOORU010000160 DAOORU010000000
VERSION     DAOORU010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18750_length_5292_cov_4.588314"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /gene="rpoB"
                     /locus_tag="PLF20_08995"
     CDS             <1..601
                     /gene="rpoB"
                     /locus_tag="PLF20_08995"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019149412.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOX81173.1"
                     /translation="LPDGTPVDIVLNPLGVPSRMNLGQIYETILGWAGKELGLRFSTP
                     IFDGASLDQINEYMGKARLPENGSLYLYDGGTGERFDQPATVGYIYMLKLHHLVDDKM
                     HARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAFGAANVLQEILTVKSDDVAGR
                     AKAYEAIVKGENMPVPNVPESFNVLVHELRGLGLNIAFE"
     gene            668..4951
                     /gene="rpoC"
                     /locus_tag="PLF20_09000"
     CDS             668..4951
                     /gene="rpoC"
                     /locus_tag="PLF20_09000"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOX81174.1"
                     /translation="MAFRKDPTAPISNFNQIMISLASPESILERSYGEVLKPETINYR
                     TYKPERDGLFCERIFGPVKDWECHCGKYKRIRYKGIVCDRCGVEVTEKKVRRERMGHI
                     QLVVPVAHIWYFRSLPNKIGALLGLPTKKLESIIYYENYVVINPGIMSSEGISYMDFL
                     SEEDYFKIIENLPPENQNLDDSHPDKFIAKMGGEALYDLLSRIDLDKESFDLRYKASI
                     ETSQQRKQDILKRLQVFEAFREANRRTENRPEWMMVKVVPVIPPELRPLVPLDGGRFA
                     TSDLNDLYRRVIIRNNRLKRLIEIKAPDVILRNEKRMLQEAVDSLFDNSRKASSVRTE
                     SNRALKSLSDSLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLNECGLPKEMASELF
                     KPFIIRKLLERGIVKTVKSAKKIVDRKDPVVWDILENVLKGHPVLLNRAPTLHRLGIQ
                     AFQPKLIEGKAIQLHPLVCTAFNADFDGDQMAVHVPLGNAAILEAQILMLASHNILNP
                     ANGAPITVPSQDMVLGLYYITKPRKSEPGHPVKGEGMVFYSPEEVVIAYNEQKVDLHA
                     IIKVRITDFTTDPPQKELIETTVGRVLFNQVIPKEIGFINQMLTKKNLRDIIGEVLKR
                     TGTAVTAKFLDDIKEMGFMTAFKAGLSFNMGDVIIPDNKDELIAEANAEVEEVIGNYT
                     MGFITNNERYNQIIDIWTHTNSKLTLTLMDKLTKDKQGFNSLFMMLDSGARGSKEQIR
                     QLAGMRGLMAKPQKSSGSGGEIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKT
                     ADAGYLTRRLVDVAHDVIINEEDCGTLRGLTVTALKKDEEIVESLYDRILGRVALHDI
                     YHPYTNELIATAGQELTEEICEIIEKSPIESVDIRSVLTCESKSGVCALCYGRNLAKG
                     KMVQKGETVGVIAAQSIGEPGTQLTLRTFHVGGVAGNIALTSRIEAKYDGRLQIDELR
                     SVEYTSESGRTYHVVLGRSAEMHIVDPNTEIILTSAHIPYGANLYFKSSSMVRKGDLI
                     CDWDPYNALIISDVPGVVAYHNLIEGVTYRTESVEQTGHPYKVVVESRQKTKNPAIRI
                     LNADGEEIKIFDIPVGAHLSVEDGQKIKAGDILVKIPRAVGKSGDITGGLPRVTKLFE
                     ARNPSDPARVSEIDGIVTFGKKLKRGNRELIVTSRVGEEKTYLIPTTKQILIQEHDYV
                     KAGQPLSEGAISPSDILAIKGPMAVQEYIVNEIQEVYRLQGVKINDKHFEVIVRQMMR
                     KVEIEDPGDSKFLEGELVNKPDFQEENDWLFGKKVVIDPGDSPNLSAGQIISTRKLRD
                     ENSMLKRKDKKLVEARDARPATARQILQGITRASLQTKSWISAASFQETTKVLTQAAI
                     NAKIDTLQGLKENVIVGHLIPAGTGLREYENIIVSSIEEYEALKASKDE"
     gene            4982..>5292
                     /locus_tag="PLF20_09005"
     CDS             4982..>5292
                     /locus_tag="PLF20_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOX81175.1"
                     /translation="MEPKKNPPNQIQVDLPEDVASGIYSNLAIITHSDSEFLIDFLSA
                     LPGLPKAKVKSRVIMTPLNAKRLLRALKDNISKFEQTHGEIMESSAHEGFPINMGPTG
                     IA"
BASE COUNT         1567 a         1116 c         1185 g         1424 t
ORIGIN      
        1 cttacccgat ggcacacccg tagatattgt tctgaatcca ttgggcgttc cttcacgaat
       61 gaaccttggc cagatttatg aaaccatcct gggttgggct gggaaagaac ttggcttacg
      121 cttttcaact cccattttcg atggtgcttc attagaccag atcaatgaat atatggggaa
      181 ggccagatta cccgaaaacg gaagcctgta tctatacgat ggtggtacag gcgaacgttt
      241 cgaccaaccg gccacggtgg gttatattta tatgctcaag cttcatcatc ttgttgacga
      301 taagatgcat gctcgttcca taggccctta ttctctcatt acacaacaac ctcttggtgg
      361 gaaggcccaa tttggaggac aacggtttgg agaaatggag gtttgggcac tcgaagcctt
      421 tggtgcagcc aatgttctgc aagagattct tactgttaaa tcagacgatg tagcagggcg
      481 agccaaggct tacgaagcta tcgtcaaggg tgaaaatatg cccgttccca atgtacccga
      541 gtcattcaat gttctggttc atgaacttcg aggtttaggc ctgaatatag cctttgaata
      601 atgccatttg gcgtaagact caggcgtttg tataaaattt ttattttgct gaaaatcaaa
      661 ttcaaatatg gcgtttagaa aagatcctac tgctccaatc agcaatttca atcaaatcat
      721 gatcagtttg gcttctcccg aatccatact cgaacgatca tacggtgaag ttctaaaacc
      781 agagaccatt aattatcgca cttataagcc cgagagagat ggcttgtttt gtgaacgaat
      841 ctttggtccg gtaaaagatt gggaatgtca ttgtggaaaa tacaaaagaa tccgttacaa
      901 gggtatagta tgcgaccgtt gcggtgttga agtaaccgag aaaaaagttc gacgcgaacg
      961 catggggcat attcagcttg ttgtccctgt ggctcatatc tggtatttca ggtctcttcc
     1021 caataaaatt ggcgctctgt tgggtttacc caccaagaag ctggaatcca tcatttatta
     1081 cgagaattat gtagtaatca atcctgggat aatgtcgagc gagggcatat cttacatgga
     1141 ttttttatct gaagaggact atttcaagat catcgaaaac cttccccctg aaaatcagaa
     1201 tctcgatgat agccatcccg ataaattcat tgcaaaaatg ggaggcgaag ccttatatga
     1261 ccttctttca cgtattgatc tcgataagga atcattcgat ttaagataca aggcaagcat
     1321 tgaaacctct caacaacgta aacaggacat tttaaaacgt ttacaagttt ttgaggcatt
     1381 tcgtgaagcc aaccgccgaa ccgaaaatcg tccggaatgg atgatggtaa aggtagttcc
     1441 agtaatacct cccgaattga ggcctttggt tccattggat ggtggccgtt ttgccacttc
     1501 cgacctcaat gatttgtatc gcagggtgat aattcgtaac aatagactga aacgactcat
     1561 cgagataaaa gcacccgacg ttatcttgcg taacgaaaaa cgcatgcttc aggaagccgt
     1621 tgattctctt ttcgataatt cacgcaaggc cagctccgtg agaaccgaat ccaatcgtgc
     1681 tctcaagtca ctgagcgaca gtttgaaagg caaacaagga cgtttccgtc agaatctgct
     1741 tggaaaacgc gtcgattatt caggccgttc cgtcatcgtt gttggccccg aactcaagct
     1801 caatgaatgc gggcttccaa aggaaatggc cagtgaactt ttcaaacctt ttattattcg
     1861 caaactactc gaacgcggaa ttgtaaaaac cgtaaaatcg gccaagaaaa tcgtcgatcg
     1921 caaagatcct gtggtctggg atatcctcga aaatgttttg aaaggtcatc ctgtattgtt
     1981 aaaccgtgct cctaccttgc accgccttgg aattcaggcc ttccagccta aactcatcga
     2041 aggcaaagcc attcagcttc atccacttgt ttgtacggct ttcaatgccg acttcgatgg
     2101 cgaccaaatg gccgttcatg ttcctttggg caatgccgct attttagaag cacaaatttt
     2161 gatgctggca tcccacaaca tcctgaaccc cgccaatgga gctccaatta ccgttccttc
     2221 gcaggatatg gttttaggat tatattatat cactaaaccc cgtaaatctg aacccggaca
     2281 tcccgtaaaa ggtgaaggta tggtatttta ttcacccgaa gaagtagtga ttgcttataa
     2341 tgaacaaaag gttgatctgc atgcaattat aaaggtcaga attaccgatt ttaccactga
     2401 tcctcctcaa aaggaactca tcgaaacaac cgttggaaga gttttattca atcaggttat
     2461 tcctaaagag atcggtttta ttaatcaaat gctgaccaag aagaacctga gagatatcat
     2521 tggcgaagta ttgaaacgta cgggtactgc ggtaactgct aaatttctcg atgatatcaa
     2581 ggaaatgggt ttcatgacgg ccttcaaggc cgggctttcg ttcaatatgg gtgatgttat
     2641 catacccgat aataaagacg aattgatagc cgaagccaat gccgaagtcg aagaagtaat
     2701 aggcaactat acgatgggct ttattaccaa taatgagcgc tataatcaga tcatcgacat
     2761 ttggacgcat accaattcga aacttacgct tacattgatg gataagctca ccaaagataa
     2821 gcaaggattc aattccttgt tcatgatgct ggattcaggt gctcgtggtt cgaaggaaca
     2881 gatcaggcag ttggctggca tgcgtggttt gatggcaaaa cctcagaaat cgagtggtag
     2941 cggtggtgaa atcatcgaaa atcccattct atcgaacttc aaagaaggct tgtcggttct
     3001 ggaatatttc atttctacac atggtgccag aaaaggattg gccgatactg ccctgaaaac
     3061 tgctgacgct ggttatttga ccaggcgcct ggttgatgtt gcccatgatg tgattatcaa
     3121 tgaagaagat tgtgggacct taagaggttt aaccgtaacg gcactcaaga aagacgaaga
     3181 aattgtcgag agcctttacg accgtatctt aggtcgcgta gcccttcatg atatttatca
     3241 tccatatacc aacgaattga ttgctactgc gggtcaggaa ttaaccgaag aaatctgcga
     3301 gattatcgaa aaatcgccta ttgaatctgt cgatatacgg tcggttctta cttgtgaatc
     3361 gaaatccggc gtttgcgcct tatgttatgg acgaaatctg gccaaaggaa aaatggttca
     3421 gaaaggtgaa accgttggag taatcgctgc ccaatctata ggtgaacctg gaactcagct
     3481 tacccttaga accttccacg ttgggggagt tgctgggaat attgccttga cctcacgcat
     3541 tgaggcaaaa tatgatggtc gcctccagat agatgaattg cgctcggttg aatatacttc
     3601 cgaaagcggc cgtacttacc atgtcgttct tggtcgttcg gccgaaatgc atatcgttga
     3661 ccccaacacc gagattatcc ttacttcggc acatattccc tatggagcca acctgtattt
     3721 caagagcagc tccatggtga ggaaaggaga cctgatctgc gattgggatc cctataatgc
     3781 cttgataatt tctgatgttc ccggtgtagt tgcttatcat aatttgatag aaggagttac
     3841 atacaggact gaatcggtcg aacaaaccgg ccatccttat aaggtagtgg tcgaaagccg
     3901 tcaaaagacc aaaaacccag ccatacgtat tttgaatgcc gatggagagg aaataaaaat
     3961 tttcgatatt cctgtcggag ctcatctttc ggttgaagat ggacagaaaa tcaaagctgg
     4021 agatatttta gtaaaaatac ctcgtgctgt gggtaaatca ggtgatatta ccggaggttt
     4081 accgcgtgtt accaaattat tcgaagctcg caatccgtcc gatcctgccc gtgtttctga
     4141 aattgacggt atagttacct ttggcaaaaa attgaaacgt ggcaaccgtg aactcatcgt
     4201 aacgagccgt gtgggcgaag aaaagactta tttgattcct accaccaaac agattctaat
     4261 tcaggaacac gattatgtaa aggccggcca acccctttca gaaggggcaa tctcgccaag
     4321 cgatattctt gctattaaag gtccaatggc tgttcaggaa tatatcgtca acgaaattca
     4381 agaagtttat cgcttacagg gcgtaaaaat taacgataag cattttgaag taattgttcg
     4441 gcagatgatg cgtaaagttg agatcgaaga cccgggcgat tcgaaattcc ttgagggtga
     4501 actggtcaat aaacctgatt ttcaggaaga gaacgactgg ttatttggca agaaggtggt
     4561 aatcgatccg ggagattcgc caaatctttc ggcgggtcaa atcattagta cacgcaaact
     4621 gcgagatgaa aattcaatgc tcaaacgaaa ggataagaaa ctggttgaag ctcgtgatgc
     4681 ccgcccagcc actgcacgcc agattcttca gggaataaca cgtgcttctc ttcaaactaa
     4741 gagctggata tcggcggctt cgttccagga aaccactaaa gtacttactc aggctgctat
     4801 aaatgctaag atcgatactc ttcaaggcct aaaggagaat gttatcgtcg gacatcttat
     4861 tcccgctggt actggcctgc gtgaatatga aaatattatc gtaagcagta tagaagaata
     4921 tgaggctctt aaagcttcaa aggatgaata acaaataata aataaatata tgcgataatc
     4981 catggaacca aaaaagaacc cacccaatca aattcaggtt gatttacccg aagacgttgc
     5041 cagcggcatc tattcgaatc tcgccataat cactcattcg gattcagagt ttttgattga
     5101 ttttcttagc gctttgccag gtttacccaa agcaaaagta aaatcacgcg taattatgac
     5161 tcctttgaat gcaaagcgct tgctacgtgc tttaaaggac aatatttcga agtttgaaca
     5221 gacccatggt gagataatgg aaagcagtgc acacgaaggt ttcccaataa atatggggcc
     5281 aacaggaatt gc
//
LOCUS       DAOORU010000161         5167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_19353_length_5167_cov_6.312402, whole genome shotgun sequence.
ACCESSION   DAOORU010000161 DAOORU010000000
VERSION     DAOORU010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19353_length_5167_cov_6.312402"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..374
                     /locus_tag="PLF20_09010"
                     /pseudo
     CDS             57..374
                     /locus_tag="PLF20_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
     gene            complement(390..1553)
                     /gene="hflX"
                     /locus_tag="PLF20_09015"
     CDS             complement(390..1553)
                     /gene="hflX"
                     /locus_tag="PLF20_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOX81176.1"
                     /translation="MIERVNKKETAVLIGLITPEQPVEKLNEYLDELEFLADTAGADV
                     RKRFVQRVPYPDPRTFVGKGKLKEVFDYVVENAIDLIIFDDELTASQLRNLDRELPCK
                     ILDRNNLILDIFAHRARTSHAIAQVELAQYEYMLPRLARMWTHLEKQRGGIGLRGPGE
                     KEIETDRRLIRKRIALLKNQLEQIDQIKQTQRKSRTNLVRVALVGYTNVGKSTLMNVL
                     SKSSVFAEDKLFATLDTTVRRVVIDRTPFLLSDTVGFIRKLPHSLIESFKSTLDEVRE
                     ADILLHVADVSHPALEEQIEIVKQTLMEIGIHQKNIITLMNKADLLFKQSSADDLTSK
                     AIPRWIIDENQPCLLISAVKQQGTQQLKEILAERVRQIYRLRYPFKTRYDDLE"
     gene            complement(1744..2481)
                     /locus_tag="PLF20_09020"
     CDS             complement(1744..2481)
                     /locus_tag="PLF20_09020"
                     /inference="COORDINATES: protein motif:HMM:NF003198.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047294 - phosphoglycerol
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0006650 - glycerophospholipid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HOX81177.1"
                     /translation="MEVYSKISVNSSEGKGMFAILIDPDKIHHNSFRTVAHKAAEADV
                     DFLFIGGSLLSADIMEECLYEVRCETDIPLVIFPGSVFQINKAADAILLLSLISGRNP
                     EMLIGNHVVAAPYLKASQLEIIPTGYMLIESGVSTTALYMSCTFPIPHSKDEIAVNTA
                     MAGEMLGLKLIYLDAGSGATSPVSASMITKVKQAIGIPLIVGGGIDTPEKARRAREAG
                     ANLVVVGNAIEKDSGLIHEIAYAIHEI"
     gene            2978..3955
                     /gene="dusB"
                     /locus_tag="PLF20_09025"
     CDS             2978..3955
                     /gene="dusB"
                     /locus_tag="PLF20_09025"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOX81178.1"
                     /translation="MKIGSLEISYPFIALAPMEDITDTSFRRICKKLGADMVYTEFIS
                     SQGLTREGEKSLEKLKFSEEERPIGIQIFGNETTSMVEAAKIATTVQPDLIDINWGCP
                     VKKVAGKGSGSGILNDIPKMISITQAVVKATHLPVTAKTRVGYDESNKPIVEITERLQ
                     DAGVAGITIHGRTRSQLFSGEADWTLIGKVKENPRIKIPIIGNGDIVSAHFAKTMLER
                     YHVDGIMIGRAAIGNPWIFDEVKTFLNSEILLPPPNINERINVLIEHLQIAIQIKGER
                     RAILEMRKHYSGYFKGMPNFKKIRTQLMTFNYFHEIDDFLQNVMQAGLT"
     gene            complement(4763..>5167)
                     /locus_tag="PLF20_09030"
     CDS             complement(4763..>5167)
                     /locus_tag="PLF20_09030"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOX81179.1"
                     /translation="NAILLDMPPERVELLKKASRHYRLFLLSNTNQIHFEKYTRDFKL
                     RYGYDFEDLFEKAYWSFRIHQSKPNPEIFKYVLEDSCLNAAETLFIDDTLENVLSARN
                     AGLIPYHLNQKDLTDLFDESGVLKPDLFLENK"
BASE COUNT         1639 a         1029 c          983 g         1516 t
ORIGIN      
        1 tcattgtgtg attgttgtaa aaatgtctct taaaatgtgt cgaagtcagg aaatttgggt
       61 atcctaccga aatcatggga gccagttttc gaaatataga tcaggtaatt gccctagctg
      121 gttgtgatct tttgaccata tcgcctgcat tactatatga actcgaaaat acggagcggg
      181 aggttgtttg caggctcgac gtaaaaaaat ctgccgaatt ggatattgct cctttgccgg
      241 cagatgaaaa atcctttcgt tgggatctta acgaagatgc aatggcaacc gaaaagcttg
      301 ctgagggaat ccgacggttt aaggacgatt tgatcaaact ggaaaatttt ctggaagcca
      361 atttcttttc ctaagttttc gatgggtttt cattccagat cgtcgtatcg cgttttaaaa
      421 ggatagcgaa ggcgataaat ttggcgtact ctttctgcca aaatttcctt taattgctga
      481 gttccttgtt gttttactgc cgaaattaac aaacaaggct ggttttcatc aataatccag
      541 cggggtatgg cctttgaggt caaatcatcg gcagaggatt gtttaaaaag aaggtcagcc
      601 ttattcataa gagtaataat atttttttga tggattccaa tctccataag ggtttgtttt
      661 acgatttcga tttgttcttc taaagcaggg tgtgatacat cggcgacatg aagcaaaatg
      721 tcggcttccc tcacttcatc caaggtagat ttgaacgatt cgatgagcga atgtggcaac
      781 tttcggatga atcccacggt atcggagagt aaaaaagggg ttcgatcaat taccacacgg
      841 cgaaccgtgg tgtcaagggt tgcaaaaagt ttatcttcgg caaatacact cgatttgctc
      901 aatacattca tcagggtaga ttttcctaca ttcgtataac ccacaagagc caccctcacc
      961 agattagtgc gtgatttgcg ctgagtttgc tttatctggt caatttgttc caattgattc
     1021 tttaacaagg caatacgttt tcgaatcaaa cggcggtcag tttcgatttc cttttctcca
     1081 ggtcctctca atccaatgcc gccacgctgc ttttcgaggt gagtccacat acgtgcaaga
     1141 cgaggcagca tgtattcata ttgtgcaagc tccacctgag caatggcatg ggaggtgcgt
     1201 gccctatgtg caaagatgtc aaggatcaga ttgttacgat ctaatatttt gcacggaagt
     1261 tccctgtcta aatttcgtaa ttgtgaggca gtaagttcat catcaaaaat aatcagatct
     1321 atggcgttct caactacata atcgaaaacc tccttcaatt tacctttccc gacaaaagtt
     1381 ctcggatcgg ggtaaggaac acgttgtacg aatctttttc taacatcggc tccagcggta
     1441 tcggctaaaa attcgagctc atccagatat tcattcaatt tttccaccgg ctgttccggc
     1501 gtaatcaatc ctattaaaac tgcagtctct tttttgttaa ccctttcaat catacatcat
     1561 ccattcccac ttgatgcacg aagcaatttt aattccaatt cgaaattttt cgaaaaaatc
     1621 atggaaggct ggattgaatg ggaaaaaatc attctataaa ttgtgtcagc gttgattatt
     1681 ctcagtcggg aatgattaaa agatcgggag ctctatcgtt gttcatacaa tttcaggcac
     1741 atgctaaatt tcgtgtatcg cataagctat ttcatggatt aaccccgaat ctttttcgat
     1801 ggcattaccc actactacga ggttggctcc ggcttctctg gcccgacgag ctttttcggg
     1861 tgtatcgatt ccccctccca caatcaaagg gataccaata gcttgtttta ctttggttat
     1921 catagaagct gaaactggcg aagtagcacc acttcctgca tccaaataaa tcaatttcaa
     1981 tccaagcatt tcaccggcca tagcagtatt tactgcaatc tcatctttag aatgggggat
     2041 ggggaaggtg cagctcatat acaaggctgt agtcgaaacg ccactttcaa ttagcatata
     2101 acccgtagga atgatttcta attgactggc cttgagataa ggtgcggcaa ctacgtgatt
     2161 cccaataagc atctctggat tcctccccga gattaagctt aagagcaaaa tggcatcggc
     2221 tgccttgttt atttggaaaa cactgcccgg gaaaataacc aaaggtatat cggtttcgca
     2281 tcgaacttca tataaacatt cttccataat atcggcacta agcaaactgc ctccaataaa
     2341 taaaaaatct acatctgctt cagcagcttt atgagctact gttctaaaac tattatgatg
     2401 aatcttatcg ggatctatca aaatggcaaa catcccctta ccttcgctac tattgaccga
     2461 tattttcgaa taaacctcca tggttctatg ctctaaggtg caaatataaa aattattttt
     2521 ttgaaagtaa tttgtaaatt ttagaagttg gaattcaaat tttgatgatt aatagttgaa
     2581 gagtcaaaaa aaaaactatg taaatctggt gaaaatcaat caaatggcta gtaattaacg
     2641 acatttcgaa atatttatta ggcaagaatt caaacttcct atgataagtt ttcaatgatt
     2701 tacactccca taaatttata ttcaaaacgg tcacactcct aatgaagtgg tcaaaagaac
     2761 gtgaaacgga ggtcggaaaa tttcccaatc tcaaaatgtt ccagtcttaa aatctcatca
     2821 tctgctttca cctcgccatc cggaccacgg aatacttctc acgcaatgct tttcacattt
     2881 ttctccaacc tacaaaatgc cttctacctc ttaattcatg ctgctttttt ctttttacta
     2941 tcttagcaat taaaatgctt atgcgactgt ttcatttatg aagataggat cattggaaat
     3001 cagctatcca tttattgcac ttgctcctat ggaagatatc acggatacat cgtttcgaag
     3061 gatatgcaag aagttgggtg ccgatatggt ttacacagaa tttatctcat cgcaaggttt
     3121 aacaagggaa ggagaaaaaa gtttagaaaa actgaaattt tcagaagaag aaaggcctat
     3181 tggaatacaa atctttggga atgaaacaac aagcatggta gaggcagcaa aaattgcaac
     3241 cacggttcaa cccgatttga tcgatattaa ctggggatgc cccgtaaaga aagtcgcagg
     3301 caagggttct ggctctggta ttcttaacga catccccaag atgatctcaa tcacccaggc
     3361 cgtagtgaag gccactcatt tgccggttac ggccaaaacc agagtgggct acgacgaatc
     3421 gaacaaaccc attgtcgaga tcaccgaaag actgcaggat gccggagttg ccggtattac
     3481 cattcatggc agaactcgtt cacagttatt tagtggagaa gccgactgga ctcttatcgg
     3541 aaaagtaaag gaaaatccac gcataaaaat tcccattatt ggcaatggcg atatcgtatc
     3601 ggcccatttc gctaaaacca tgctcgaacg ctaccatgta gatggtataa tgattggacg
     3661 ggctgctatt ggcaatccct ggatattcga cgaagtaaaa accttcctca attctgaaat
     3721 tttgcttcca cctcctaata ttaatgaaag aatcaacgtt ttgatagaac atttgcaaat
     3781 agccatccaa ataaaaggcg aacgcagagc tatcctcgaa atgagaaaac actacagcgg
     3841 ctatttcaag ggaatgccca acttcaagaa aatccgaaca caactgatga cattcaatta
     3901 tttccatgaa attgatgatt tcttacagaa tgttatgcag gccggcctca cctgaaatga
     3961 cttctataaa agaaaattag aacacagtca atggtatatt caaaatttat taatagccct
     4021 acctcctatc tatatccctt catttccttc taattatgta aaactaacag catcaaaatg
     4081 atgatatgtt aaatttttat aatagaagat ggtatggtta caccaaaaat tctttccgaa
     4141 gccttaagaa aggaatggga ttttgaaatt gtgtccctgt tttctggtaa aaagctttta
     4201 aagcgtctcc cggaagcacc taatataatt tcgcacgaat attatttact tgaccacttg
     4261 ggatcataac ttttcgagga aatcaaggag ctataattcg agaattctaa tgatcatcgt
     4321 aagcggacag caaaacatag ctgtggctaa gaatatcatt cacaaggttg cttatgagta
     4381 tatgaggaag gacccgtaac atgagaaccg gctttatctt aatattacca catagaataa
     4441 aaagaatacc gaaaagaaaa tagaagtatg ggtataagaa accgcaaaac aatacatcga
     4501 atagaatccg ctgaaagaaa taacccacaa atatcgatgg tgtaagagcc aataaagaag
     4561 acagccccgt tgaatattgc attctcatta caggaaaacg ctcgaaaaaa tatctaaaat
     4621 aaattgtaat ggcttatttt aaaaatacaa agtcaaagcg aaaaaagtag ctgcataatt
     4681 aagagtcggt aaggcaagca tttactggat gattaataag aatctgcata tttgcgaaat
     4741 taagtcatca catagacttc cttcatttat tttctaaaaa taaatcaggt tttaacactc
     4801 ccgattcatc gaacaaatct gtcaaatctt tctggttcaa atgataagga attaatcctg
     4861 catttctggc gctgagcaca ttttcaaggg tatcatcgat gaataaagtt tcggcagcat
     4921 tcaaacatga atcttccagc acgtatttaa aaatttcagg attgggtttg gattgatgaa
     4981 ttctgaaaga ccaataagcc ttttcgaaca gatcttcaaa atcgtaacca taacgcaatt
     5041 taaaatctct ggtatacttt tcgaaatgaa tttgattggt attactaagc aagaaaaggc
     5101 ggtagtgccg gcttgctttt ttcaataatt caactctttc gggaggcata tccagcaaga
     5161 tggcatt
//
LOCUS       DAOORU010000162         5165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_19364_length_5165_cov_5.498826, whole genome shotgun sequence.
ACCESSION   DAOORU010000162 DAOORU010000000
VERSION     DAOORU010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19364_length_5165_cov_5.498826"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1912
                     /locus_tag="PLF20_09035"
     CDS             <1..1912
                     /locus_tag="PLF20_09035"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOX81180.1"
                     /translation="RLGLDQRSQFELYEDLPGGLKQLKHQAELAHEMGKKYFLSYNPW
                     DKPEQEKFHRTNLKKLLEQTDADGLVLDTKGESDAWLQNLADSVKPGIIMYSEGMAVP
                     KNMTGIVAGRVHDALYMPPVLNLNKLIKPDFAIFRVLQVADGELHREIAAAFFNGYGV
                     EINTMRPGRPASLQDDLFFLGQTTKILRDNQSVFFNYNYLPLIHTQMDSIYVNKWETD
                     EKIIYTIYSLRPTGFRSPLFGFDELQVTNSSITEEPYHFVSLWNHEEIQAVKMDGKYY
                     LPVNVASFDRAFLGTRSEAQVECIAVLPKLLEVDLEHDTLYFGSSFGNRIEITAGNPS
                     YSSRTFPFDMGKGFISLYETFGRYEGKIVIQLYGGNELLDERIVKIDPGSPRLIVRQE
                     QTNKPAIAPEDMIFIPGGRYDFVSYDDEDDDEPFISCPYHNDTLHLIMKSFYMDKYPV
                     TNQQFKNFLKKSHYFPVDTTNFLRHWKNGEIPSGMENHPVVYVSYQDAKAYADWTGKR
                     LPTEQEWQYSAQGSSFRKWPWGDTFDASRCNFNVYHTTAVDAFPEGISPFGVMDLVGN
                     VWQITGDVYDNGAYYFNVIKGGSYYKPGKSQWYLKGGPRPLSHSQIQLMVGPSFDRSS
                     TVGFRCIMDAIE"
     gene            1989..3191
                     /locus_tag="PLF20_09040"
     CDS             1989..3191
                     /locus_tag="PLF20_09040"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOX81181.1"
                     /translation="MDYLSKRVKNMEESATLAMTSKSRELASQGLDVINLSIGEPDFN
                     TPEFIKEAGKKAIDENYTHYPPVPGYQDLREAICIKLRRDNQLEYKPNQIVVSTGAKQ
                     ALANCILSTVDPGDEVIIPVPYWVSYTELVKLAEGKPIFVKATVDNHFKITAKQLEEA
                     ITPRTRLLIFNSPNNPSGSVYNWQELKSLAEVLARHPQVLILSDEIYEYIVFSGKHCS
                     LASFPEIKNRVALINGVSKGYAMTGWRLGYIAAPQNIASACNKIQGQITSATSSISQR
                     AAIAAMLRHPDETPELQQMVSAFIQRRDIVLNLLAEIPEIKTNVPEGAFYVFADFSAF
                     LAKSDGKKQIHSIDDLCMYLLEKALVALVPGTAFGDPQCLRISYATSENNLVKALQRI
                     KEALALLH"
     gene            4110..>5165
                     /locus_tag="PLF20_09045"
     CDS             4110..>5165
                     /locus_tag="PLF20_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81182.1"
                     /translation="MKINLINFRKLSWRTLITFIIFVISGYFSFSQTVTELVVPKYIG
                     AKNASGINNERTPFAVCYQIMGLDPNQVYDVKTQIGLVTEASTVWGAGNVWDPISGSF
                     SGNTVVDAFTTNASGSAIFWIYYQPTGNSSRFGAGQQHNLRLGWRPSGGSFPSNPQFV
                     GSKIITALDIAPNATTPETTDDGAYIKGTANPAASGKFVLLFDNVNGTGDPLFSYQIR
                     QALPVNTNQSMLNPEINDIYLQAGTSIIGDYPAVVPISANNPNGIRRIESRNDDNAIY
                     AFSTDADGIWPSGANTTNMNRLDIRYITMTDAPLIPQGIPTVTTVATTDITYNSATSG
                     GTVTAAGGSSVSARGVCW"
BASE COUNT         1509 a         1066 c         1071 g         1519 t
ORIGIN      
        1 tcgccttgga ctcgaccaga gatcacaatt cgagctctat gaagatcttc ctggtggatt
       61 gaaacaactc aaacatcaag cagaactcgc tcacgaaatg gggaagaaat acttcctttc
      121 ttacaatccg tgggataaac ctgaacagga aaaatttcac aggaccaatc tgaagaagtt
      181 gctcgaacaa accgatgccg atggtttggt actcgacaca aagggcgagt cggacgcctg
      241 gttgcagaat cttgccgaca gcgtaaagcc agggattatc atgtattcgg aaggaatggc
      301 cgttccgaag aatatgacgg gaatagttgc cggaagagtt cacgatgcac tctatatgcc
      361 tcccgtcttg aatctgaata aactcatcaa acccgatttt gccatatttc gtgttctgca
      421 agttgccgac ggagagttac atcgcgaaat tgctgcggct ttttttaatg gatatggagt
      481 agaaatcaac accatgcgtc caggcaggcc tgcttctctg caagatgacc tcttctttct
      541 gggtcaaact accaaaattc tgagagacaa ccagagtgta ttcttcaatt ataattattt
      601 acccctgatt catacacaga tggatagcat ctatgtcaac aaatgggaaa ccgacgaaaa
      661 aatcatatat accatctaca gtttacgccc tactgggttc cgtagcccct tattcggatt
      721 cgacgaattg caggtcacca attcaagcat cacagaagaa ccttatcatt ttgtcagcct
      781 ttggaatcac gaagaaattc aagccgtaaa aatggatggg aaatattatt tacctgtgaa
      841 tgtggcttcg ttcgatcggg cctttttggg aacccgaagt gaggctcaag tagaatgcat
      901 agccgttttg cctaagcttt tggaagtcga cctggaacac gatactttgt attttggttc
      961 ttccttcgga aatcgcatcg agattactgc cgggaatcca tcttattcga gcaggacctt
     1021 ccctttcgac atggggaaag gttttatttc actctatgaa accttcgggc gttacgaagg
     1081 gaaaatcgta atccagctct atggagggaa tgaattatta gacgagagga ttgtgaaaat
     1141 cgacccagga agtccaagat tgattgttcg ccaagaacaa accaataaac cagccattgc
     1201 tccggaagat atgattttca ttccgggagg gagatatgat tttgtatcct atgatgatga
     1261 agatgatgat gaacctttta tttcttgtcc ttaccacaat gacactcttc atttaatcat
     1321 gaaatccttt tatatggata aataccctgt taccaatcaa cagttcaaaa attttctcaa
     1381 aaaaagtcat tattttcccg ttgataccac gaacttcttg agacattgga agaatggtga
     1441 aattccgtcg ggtatggaaa atcatcccgt tgtctatgtg agttaccaag atgctaaagc
     1501 ttatgccgat tggactggga aacgactgcc tacggaacag gaatggcaat attcggctca
     1561 agggagcagc tttaggaaat ggccctgggg tgataccttc gatgcgagcc gctgtaattt
     1621 caatgtttat catactacag ccgtcgatgc ttttcctgaa ggcataagtc cattcggtgt
     1681 gatggatctg gtaggcaatg tttggcaaat tacgggcgat gtttacgaca atggggctta
     1741 ttatttcaat gtgatcaagg gaggcagtta ttataagcct ggcaaaagcc aatggtattt
     1801 gaaaggaggt cctcgtccac tttctcactc tcagattcaa cttatggttg gcccttcgtt
     1861 cgatcgttcc tctacggtgg gatttcgctg cataatggat gcaatagaat gacaatagga
     1921 tttttaagtt atttcctaca tttgcgcttt cttaatggag aataccttca ctaaaatata
     1981 agaattttat ggactatttg tcgaaacgtg ttaaaaacat ggaagaatcg gccacactcg
     2041 ctatgacgag caaaagccgt gaattggcca gtcagggttt agatgtcatc aatcttagca
     2101 ttggtgaacc cgatttcaac actcctgaat tcatcaagga agctggcaaa aaagccattg
     2161 atgaaaatta cactcattat cctccggtcc ctggttatca ggatttaaga gaggcgatat
     2221 gcattaaact tcgtcgagat aaccaactgg aatacaaacc caatcaaata gtagtttcta
     2281 ccggtgccaa gcaggctctg gccaattgta ttctaagtac tgtcgatccc ggagacgaag
     2341 ttattatccc tgtaccctat tgggtttctt atactgaact ggttaaacta gctgaaggca
     2401 aacctatttt tgtaaaagcc actgtggata accattttaa gattactgcc aaacaattgg
     2461 aggaagcaat tactcctcgt acccgattgt tgatattcaa ctctcccaac aacccctcgg
     2521 gttcggttta taactggcaa gaattgaaat cattagccga agtcctggct cgtcatcctc
     2581 aggttcttat cctttctgac gagatttatg aatatattgt tttttcgggg aaacattgta
     2641 gcctggcgtc tttccccgaa ataaaaaata gagtggctct gatcaatgga gtatcgaaag
     2701 gatatgccat gacaggctgg cgtttgggtt atattgccgc tcctcaaaat atagcgagtg
     2761 cttgtaataa gatacagggt caaattactt cggcaacgag ttcaataagt caacgtgctg
     2821 caattgctgc catgctccgg catcccgacg aaactcccga actccagcaa atggtttctg
     2881 ccttcatcca acgtcgtgat atcgttttga atcttctggc cgaaatcccc gaaataaaaa
     2941 ccaatgttcc ggaaggtgca ttctatgttt ttgccgattt ttcggctttt cttgctaaat
     3001 ctgacggaaa gaaacaaatt catagtatag atgatctatg catgtatttg ctcgaaaaag
     3061 ccctggtggc actcgttccc ggaactgcct tcggcgaccc acagtgcctg cgtatcagtt
     3121 atgcaacatc cgaaaataat ctggtaaagg ctctgcaaag aataaaagaa gcgttggcct
     3181 tgttgcattg atgcattcgc ctctgttttt aaccttcatc ttttggaggt aaaaaccctc
     3241 ttgtcatctt cttcgaatgc ttaaaatttt tcagaaatta ctgtatctga aaaaaggacg
     3301 atcattacca aattcgatat ttctaaactt tcaagcctaa tcgattgtta ttcccaattt
     3361 actcgaatga gtctattcct tttaaatttt aggaagatgg attcatattc cttatcaaat
     3421 attattaacc cacaaattct tgatgattga taccggcatt tttatacaga aaaatcattt
     3481 gcccaatgtg tgtcgatttt tgtttttgaa atatgaaccg actgaaaagg attagagcgc
     3541 aaaccgaagt cagatgatcg ctggtgaatt caaagtacgg gaatttatca gtaattaccg
     3601 ccaatgagga tatctatttg aggaaaccaa tctattcaga accgggcgga aatattcacc
     3661 cgatcacaat atctcgaact gtgaatgagc ctgatggaag ttttcttatc attatcgttg
     3721 cgaatttcag aagatttttt cttattctca tcatccattt ttcccaaagt tgaattcctg
     3781 agatgcacaa taacatagaa catcctatag ctctgaaggg gaaaatagca aaatattcca
     3841 tagtatatat gatgtagata tatattagct tatggtacga tcatctcctg atccaaggat
     3901 gagagttata ggtttccatc tgatgataga atttgctgag aaatcctttg caaatgatta
     3961 acaatctgac aattcccgaa aaaactattg ttggtgttgt ggtagttaga aaaattatat
     4021 taatttaata caagattcaa actgaattca accaaatcgt ttatttttac actgtaaatc
     4081 catcaattat taactaatta atattcaaca tgaaaataaa tttaataaat ttcagaaagt
     4141 taagctggag aacactgata acctttataa tatttgtgat aagtggttat ttttcgtttt
     4201 cgcagacagt aaccgaattg gtggtgccca agtatatagg tgctaaaaat gcttcgggca
     4261 taaataatga acgtacgcca tttgctgttt gttatcagat tatgggtttg gatcccaatc
     4321 aggtttatga cgttaaaaca cagattggcc ttgtaacgga agcctcgact gtttggggag
     4381 ctggcaatgt ttgggatcct atttctggct cattttcagg gaataccgtt gttgatgcct
     4441 ttactaccaa tgcttcggga agcgctattt tttggattta ctatcaaccc acaggcaata
     4501 gttcgcgttt tggagccggg caacagcaca acttgcgatt aggctggagg ccctcgggtg
     4561 ggtcattccc atcgaatcct cagttcgttg gtagtaaaat aattaccgct ctcgacattg
     4621 ctcctaatgc tactaccccc gagactaccg atgatggggc ttatatcaaa ggcacagcca
     4681 atcctgcagc aagcggaaaa tttgtattac ttttcgacaa tgtgaacggt acgggcgatc
     4741 cgttattttc ctatcaaatt cgtcaagctc tgccggtcaa taccaatcag tctatgctca
     4801 atcccgaaat caatgatatt tacctgcaag ctgggacaag cattataggc gattatccgg
     4861 cagttgttcc aatcagtgcc aacaatccca atggaatcag gcgcatagag tcgcgcaacg
     4921 atgacaatgc tatctatgca tttagtaccg atgccgatgg aatctggcct agcggtgcca
     4981 atactaccaa tatgaatcgt ctcgatatac gctatatcac tatgactgat gctccattga
     5041 tacctcaggg tatacccacg gttacaactg ttgcaaccac tgatattact tacaatagtg
     5101 ctaccagtgg tgggacggtt actgctgcgg gaggaagttc ggtatctgcc agaggcgttt
     5161 gctgg
//
LOCUS       DAOORU010000163         5143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_19476_length_5143_cov_5.087068, whole genome shotgun sequence.
ACCESSION   DAOORU010000163 DAOORU010000000
VERSION     DAOORU010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19476_length_5143_cov_5.087068"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="PLF20_09050"
     CDS             <1..525
                     /locus_tag="PLF20_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HOX81183.1"
                     /translation="LTHHSYFNLSGFTQRNILEHELIIHADSFTPVDSTLIPTGEIVP
                     VKNTVFDFTSPRPIGSRIREVFNKKTGYDNNYVLNTTDSTVHPAAKAYDPESGRILEV
                     FTDQPGLQFYSGNFLDGKIKGKNGKIYQQHDGFCLETQHFPDSPNHPEFPSTVLRPGQ
                     IFRSLTIYKFSTDR"
     gene            988..1467
                     /locus_tag="PLF20_09055"
     CDS             988..1467
                     /locus_tag="PLF20_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX81184.1"
                     /translation="MKTSARFLLPLLIILISSIGVDVFGQTITISLMDGTSNTYPLTS
                     ISKIYFEGNNLIFNEANMPIALDQIRKITFTASSGINIVNSGNTSLVISPNPASDAIK
                     LFNLPNETCTVYIFDLTGLLVLSCILNPSNTSINVNNLTKGIYLVKVMNSTAKFIKL"
     gene            1550..3766
                     /locus_tag="PLF20_09060"
     CDS             1550..3766
                     /locus_tag="PLF20_09060"
                     /inference="COORDINATES: protein motif:HMM:NF025620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HOX81185.1"
                     /translation="MKKIILLLSFLTLGTLDLFAKDYLFIFSGDKSVNAIDRADIDSI
                     KFTSNQTVLSIYKDNEVIASYPLSKIDSLTFGSIDVNEVMIIYSESGIVITNPFANKG
                     INISVNGDDVTVNSKLSNISVDYILSGTTSSGSLKLYSIYKYQLTLNGVNMTNNDGPA
                     INLQSSKMCTLVLSEGTTNILADGATYASSSEDQKATLFSEGQIIFIGDGTLNISSIS
                     KHAIASDDYISLKGGNINVSSAGKDAIHVNDYFTMSDGILQLTPSGDGIDSEGHIDIS
                     GGSITFTSSVANVKAISADSTINVCGGIINLTLKGNAAKGLKGKQDMTLSGGTFTINT
                     SGAAILEASGSGYDPSYCTAIKCNHSIYINGGTFTITATGTGGKGISADNNIEITNGS
                     ITISTSGGGAIYTNTSGKTDAYNATCLTSDGNIVIIDGTIALSSSGAGGKGVSSDGIF
                     TLGSSSTSPTLSITTTGSEISLGNTSYSSPKALKADGDIKFVGGTTLISSADDGIKSE
                     TNVYVNGGNIVVSKSYEAIEAPFIYINGGITDITATNDAINATKGLVSGGTEQNDGSC
                     LYVTGGILIASCTNGDAIDSNGDILITGGLTIANGPSNGIEEAVDFNGIFNMNGGIFI
                     GAGSNSNMTKPMSNSSTQPNMFISSSSLISSSTFLNIQINSTDVITFKPKYGGYKFLI
                     SSPDMTKGASYKIYTGGSYSTSINTGGYYTGGTYTPGNLKKSGTLSLSSTVNTINL"
     gene            complement(3990..4628)
                     /locus_tag="PLF20_09065"
     CDS             complement(3990..4628)
                     /locus_tag="PLF20_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81186.1"
                     /translation="MYENVLIINKKHKKAAKTILEKVLAEKTEKYIIAISGEVGAGKA
                     EIAHVLGRKLKKRGIKLKTMYLDNYYKIPPALRTEWRKEHGLDSIGPHEINWEQVKKN
                     VHDFIEGNRSIMPLVDLMNDQVDQLMTDFKNIEILIISGLYAIELEEADLKVFIELTY
                     RETLDVQRESEKEELDDFRMKVLEQEHKAVQALKHKADFFVDFDTALEIFHL"
     gene            complement(4670..>5143)
                     /locus_tag="PLF20_09070"
     CDS             complement(4670..>5143)
                     /locus_tag="PLF20_09070"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HOX81187.1"
                     /translation="LPKDYLKKNFWYYLNRTSHGSTLSRVVHAKLAHLVGEEKLAWQL
                     FREALISDYIDIQGGTTAEGIHAGVMAGTVWLALTVYAGLSLCEDIPSFQPDLPYDWE
                     AMKFNFIFRGIRYYVEIDRQKIVLTAGAPHNIEVKAKVWGKEYILLPGIAKTIEI"
BASE COUNT         1575 a         1103 c          911 g         1554 t
ORIGIN      
        1 cttacccatc atagttattt caatctcagc ggttttaccc agcgcaatat cctcgagcat
       61 gaactgataa ttcatgccga ttctttcaca cccgtcgata gtactttgat acccaccgga
      121 gaaatcgttc cggtaaagaa taccgtcttc gattttacat caccacgtcc gattggcagc
      181 cgaatcagag aagtattcaa caaaaaaaca ggctatgaca acaactatgt tttaaatacg
      241 acggattcca ctgttcatcc cgccgctaaa gcttatgatc ctgaaagcgg ccgtattctc
      301 gaagttttta ccgaccaacc aggattgcaa ttttattcgg gaaatttcct cgacggaaaa
      361 attaaaggga aaaatggaaa aatctatcaa caacatgatg gcttttgctt agaaacacaa
      421 catttccctg acagtccaaa ccatcctgag tttccttcca ccgtgcttcg acctggccag
      481 atattccgat cgctaaccat ctataaattc tcgaccgatc gttaataagg ccttcaagca
      541 tccgtacacc ctgaataacc taccatcttt taatctaacc ccaaaccccc ctccattccc
      601 ccattatccg tcttccattt tcccgcattt ggcgatgttt ttcacttttc ttagaattac
      661 ctttaatctc tatgacgttt aattaatgtt tattctaaat gtttgaggaa ttcaacctat
      721 cctccattga gaaaatgaga tggttagagc caaagaaaac agtaaaaatg aaaaatttcg
      781 gtttttcaag caacaaaaca acttaacaaa tataagccat gaatggagaa aatcaaaatc
      841 cattgcaaac gaggaagata ggatttatca tataaattaa tctctgactg taaaaattca
      901 atcgaacttg tatataattt tgttaagtgc tttgatgttt aaaaattaaa atctgtttgt
      961 atcataaaat tttaataaag ccatttcatg aaaacttcag cgagattctt actacctctt
     1021 cttataattt tgatttcgtc gataggggtt gatgtatttg gtcaaaccat aaccatttcc
     1081 ctcatggatg gaaccagcaa tacttatcct cttacctcca tttcaaagat ttatttcgaa
     1141 ggcaacaacc tcatttttaa tgaagccaat atgcctattg cactcgatca aatacgtaaa
     1201 attacattta cggcttcatc aggaataaac atcgtaaatt ctggtaacac gagcctcgtc
     1261 atcagcccca accctgcgag tgatgccatc aaactgttca atcttcccaa tgaaacatgc
     1321 acggtctata tatttgatct gaccggttta cttgtgttat cgtgtatcct caatccatcc
     1381 aacacatcca taaacgtaaa caatttaacc aaaggaattt atttagtaaa agtcatgaat
     1441 tcgaccgcta agttcatcaa actttaaaat cttccaacaa tcaaactaat taagattgtc
     1501 cagaacaata aagccgataa ataatatcca catttgaaaa ttcttatcca tgaagaaaat
     1561 aatcctactc ttatcatttc tcacattggg aactcttgat ttatttgcta aagactattt
     1621 gttcatcttc tcgggcgata aatcagtaaa tgcgatcgat cgggcagata tcgacagcat
     1681 aaagtttact tcaaatcaaa ccgtactttc catttataag gataacgaag taattgcaag
     1741 ctatcccctt tcgaagattg acagtttgac atttggcagt attgatgtca atgaagtgat
     1801 gataatttat tccgaatctg gtatagtcat aactaatccc tttgctaaca aaggcattaa
     1861 tatatcggtg aatggtgatg atgttaccgt taattccaaa ctctccaata tctcagtcga
     1921 ttatattctt tctgggacaa cctccagcgg ttctttgaaa ctctacagta tttacaaata
     1981 ccaacttaca ttaaatgggg tcaatatgac caataatgac ggtccggcca tcaatctgca
     2041 atcgagcaag atgtgtacac tcgtgctttc tgagggcacg acaaatattc tggctgatgg
     2101 ggcaacttat gccagcagtt cggaagatca gaaagccacg ttgttttcgg agggtcaaat
     2161 tatttttatt ggagatggga cactgaatat atcgagtatt tcgaaacatg ccattgcaag
     2221 cgatgattac atttccctga agggtggaaa tatcaatgtt agttcagctg ggaaagatgc
     2281 cattcacgtg aacgattatt tcacaatgag tgatgggatt cttcaactca ctccttcggg
     2341 tgatggaatc gattcagaag gacacatcga tatatcaggc gggagtatta ctttcacgag
     2401 ttctgtggca aatgtaaaag ccatttcagc agatagcact atcaacgttt gcggaggtat
     2461 catcaatctt actttaaagg ggaatgcagc caaggggcta aagggcaagc aagacatgac
     2521 gctttcgggg ggaacattca cgatcaacac ctcgggggca gccatattgg aagcttccgg
     2581 ttcgggttat gatccctcct attgtacagc catcaaatgt aatcattcga tttatatcaa
     2641 cggcggaaca tttactataa cggctacagg gacaggtggc aaaggtattt ctgcggataa
     2701 taatattgaa attacaaacg ggtcgattac aatttcaacc tcaggcggtg gagctatcta
     2761 tactaacact tctggcaaga cagatgctta caatgctact tgcctgacgt cggacggaaa
     2821 tatcgttata atcgatggaa cgatcgcttt atcttcgtcg ggagcgggag gaaaaggcgt
     2881 ttcaagcgat gggatcttta ctctggggag ttcttccact tcgcccacac tttctattac
     2941 caccacggga agtgaaatta gcttagggaa cacaagttat tcatcaccaa aagcgcttaa
     3001 agcagatggt gatataaaat tcgtcggtgg aacaacactt atctcttcag cagatgatgg
     3061 aataaaatca gaaaccaatg tatatgtgaa cggtggcaat attgtagtca gtaaatctta
     3121 cgaagccatc gaggcgcctt ttatctatat caacggagga attactgata ttactgctac
     3181 gaatgatgcc ataaatgcta ccaaaggcct ggtcagcggc ggaacggaac aaaatgatgg
     3241 aagttgtttg tatgttacgg ggggaattct catagcctca tgcacaaatg gcgacgcaat
     3301 cgacagcaac ggggatattc tgataacagg tggcctaact atagctaatg ggccatctaa
     3361 tggtatagaa gaagccgttg attttaacgg catatttaat atgaatggcg gaatctttat
     3421 tggtgcaggc tcgaattcca acatgacaaa acctatgagt aattcatcca ctcagcctaa
     3481 tatgttcatc tcttcgagct cattgatctc ttcttctaca tttctgaata ttcaaatcaa
     3541 ttcgaccgat gtcattacct tcaaaccaaa atatggtgga tataaatttc ttatttcctc
     3601 acccgacatg accaaaggcg cttcgtacaa aatctataca ggtggttcct attcgacttc
     3661 tatcaataca gggggatatt atacaggcgg aacatacaca cctggaaact taaaaaaatc
     3721 aggaaccctc tccttgtcga gcacagtaaa tactatcaat ttatgaaata gcaccagtca
     3781 gctttagata attagataat aaaaagaatg ttcctctttt tatacctatt ttatattttg
     3841 gttaaataca ccactccttc ttacttagtc aaaatcgtat ataaggatat tttggatgaa
     3901 attcatttaa tcgcagcctc attttaatac cctgcgccgt gcacatatat aatgtcttct
     3961 aatgccttcg cccttcagtg aatttcaact taaagatgaa agatttcgag agccgtatca
     4021 aaatcaacaa agaaatcggc tttatgtttt aaggcttgca cggctttatg ttcttgctcg
     4081 agcaccttca tacggaaatc atccagttct tctttctccg attcacgttg cacatcgaga
     4141 gtttctctat aagtgagttc gataaaaacc ttcagatcgg cttcttcgag ttcaatagcg
     4201 tatagtccac taatgattag aatttctata ttcttaaaat cagtcatcag ctggtctacc
     4261 tgatcattca tcaagtcaac cagaggcata atcgagcgat tcccttcgat gaagtcatgt
     4321 acattcttct ttacctgctc ccaattgatt tcatgagggc caatggaatc gagtccatgt
     4381 tcctttcgcc attctgtacg taaggctgga ggaatcttgt agtaattatc cagatacatg
     4441 gtttttaatt taatgccgcg cttctttaat ttcctcccca atacatgcgc aatttcggct
     4501 ttaccagccc caacttcgcc tgaaatggcg ataatgtatt tttcggtttt ctcggctaat
     4561 accttttcaa ggatcgtttt agctgccttt ttatgctttt tgttgatgat gagaacattc
     4621 tcgtacattt tccttcaatt ttccgtttcg aaacgtacaa caaaactaat tagatttcga
     4681 ttgtttttgc aatgccaggc aacaaaatat attcctttcc ccacactttc gccttgactt
     4741 cgatattatg tggtgcacca gctgtaagaa ctattttttg cctatctatt tctacatagt
     4801 atctgatgcc tcgaaaaata aaattaaatt tcattgcttc ccaatcgtat ggtaaatcag
     4861 gttgaaacga agggatatct tcgcacaggc tcagtccagc ataaacagtt aaggcaagcc
     4921 agacggttcc ggccattaca cccgcatgaa ttccttcggc tgtagtcccg ccctgtatat
     4981 cgatataatc actgattaaa gcctcacgaa acagttgcca tgccaatttc tcctcaccta
     5041 ccaaatgagc cagtttggca tgaactactc tgctaagcgt gctgccatgt gaggtacgat
     5101 ttaaataata ccaaaaattt ttctttaaat aatctttggg taa
//
LOCUS       DAOORU010000164         4969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_20313_length_4969_cov_5.788360, whole genome shotgun sequence.
ACCESSION   DAOORU010000164 DAOORU010000000
VERSION     DAOORU010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20313_length_4969_cov_5.788360"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            220..906
                     /locus_tag="PLF20_09075"
     CDS             220..906
                     /locus_tag="PLF20_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX81188.1"
                     /translation="MSLNVNDGEIIAMIGPNGVGKSTTLKVICGLVLPHFGEILFQGK
                     KINGSQPYQLVRKGLSLVSEGRKVFSSMTVMKNLEMGGYILKKNIANSVNGKNKLKNS
                     IKNIFEIFPVLKERRKQKAGALSSGEQQLLAIARALILNPKLFLLDEPSLGLSPNNVK
                     IVFEKIREINKKGTTILVVEQNARMALEYADRGYIFEVGGIIMNDTAKNLLNNEKVKK
                     SFLGEINYFG"
     gene            complement(1021..1995)
                     /locus_tag="PLF20_09080"
     CDS             complement(1021..1995)
                     /locus_tag="PLF20_09080"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOX81189.1"
                     /translation="MKSFKNILSQLLKAIIAVGLIWLIIKRTGFTFNEFVDIMRGINL
                     WLYLLALPGVLIVLWLKSRRWHYLMKTEGIHYDTTNTYKSYLTSYSIGLLTPGRFGEI
                     VRVYYIRDLSEGGLFKAFQTVIADRFFDLYFVLGGGLLSYALLLQTENITFLWISVMI
                     VIFGLLILWVLLYCFRNKNQKHNFISFLYGSLYPITGIHSMKNWLLTVFAYMIYFLQS
                     YLIAKALNFHIGFWEMAGVIIITSAVLLIPITWAGFGTRELSLVVLLAHWGISSESAM
                     AFSLLQFVATFFIGGITGLVIWFSHPIPREALKKDYESIKQFLKFRKN"
     gene            complement(2006..3328)
                     /locus_tag="PLF20_09085"
     CDS             complement(2006..3328)
                     /locus_tag="PLF20_09085"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HOX81190.1"
                     /translation="MLGNASKHLECIMRANLCNRCGTCVGLSNGKILFQDREGKYRPK
                     IISPLTQEEAATIWSACSGHEFHFPEFNQWLFASASHFNRLMGSYENLYIGHANDQIV
                     RSNAGSGGILSALLIYLLEKGEIQGAVTLRMSKEKPWLTEPFIATTKEEILESAQSKY
                     VISSVNEILPQIAKFDGKLAYVGLPGQVQAIRKLQKMKHPSVDKILYIFGPFYGNTLH
                     VSSIRSFLAAHKEKDLTSIEKIYFRYGEWPGRMRIEMKNGRIMELPKFHANYLIPFHI
                     LKNSLLCTDLTNEFTDISGGDAWAPVYEERGKGFSLVIARSKQGQNLLEEMANQHIIT
                     LWPIAEEEAIKMHSHGYDLKKRGTFIRMQFRSVLGLKNPDYGYKISGFSLGRYLMEIL
                     IDFLFLLLGTKPARYIADHIPQKTMGYIFENARKYWKRATYSTKREKL"
     gene            complement(3309..4040)
                     /locus_tag="PLF20_09090"
     CDS             complement(3309..4040)
                     /locus_tag="PLF20_09090"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOX81191.1"
                     /translation="MKYKLPKNKWTDKQVEEHWDKVAHKYVKENNKVKYAHDQRFRKM
                     LELFPPLPSGAKILNITSRDAEASIFLQRTYPGIRVINAEISQGLMNEAFRLWPDIEQ
                     IKISTYSSLPFVDEEFDAIVSLETLEHVAEPVAFLKELHRVIRPSGILILSCPPATSE
                     FPYRIFTFLFGGHGEGPHRFLPSKKVKMLLKDTGWQVLHHEGTLLIPAGPVFLQKLGD
                     FLIRKFQHSIIRELGIRQFYVGKRI"
     gene            complement(4044..>4969)
                     /locus_tag="PLF20_09095"
     CDS             complement(4044..>4969)
                     /locus_tag="PLF20_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOX81192.1"
                     /translation="SIFSHSTWRQYLCQNEFICMSNTPFISVIIPAYNEEEGLRNTLD
                     KINSLHLFDRFEFIVVDDGSTDQTTFVAQQYPVRLLKHETNKGYGAALKTGIRKAKGE
                     KVIFMDSDGQHDPAFLPKIAQMLQDYDMVIGERTSDSYQVKARTSGKKIIRIVGEYLV
                     EQKLPDFNSGFRGFDRQLILSLLHIMPNGFSFSTTSTLAFLKEGYSIGTFPIHVKERQ
                     GRNSTVKFLKDGPKTFLLLFRMTMLFNPLKIFFPISVIAFIAGLGWGIHGYVVFSRFP
                     NTATILILSSMFLFLIGLLADQISVMNRRKY"
BASE COUNT         1560 a          926 c          970 g         1513 t
ORIGIN      
        1 ttttaaagaa ggaaaagcta atgtataact aactgaataa gtggtatata aaaaaatgtt
       61 taggaacttt ttgaattttg tcggttaata atgctaattt atattattcc aacattagaa
      121 atgaatgaaa atctgaatat ccaaaaccaa gaatctaaaa tcatattgca agtaaagaat
      181 gtaagtgtat aatatggatc tctgcttgct ttaaataata tttctctaaa tgttaatgat
      241 ggagagatta ttgcaatgat tggtccaaat ggtgtgggta agtcgacaac tttaaaggtt
      301 atttgtggct tagttttacc tcattttggt gagattttat ttcaaggtaa aaaaataaat
      361 ggcagtcaac cttatcaact tgttagaaaa ggattatccc ttgtatctga agggaggaaa
      421 gtgttttcat caatgactgt catgaaaaat ctcgagatgg gaggttatat tttaaagaaa
      481 aatattgcaa attcagtaaa tggaaagaat aaattaaaga atagtattaa aaatatattc
      541 gaaatttttc ctgtattaaa agaaagaaga aaacaaaagg caggcgcact aagcagtggg
      601 gagcaacaac tacttgccat tgctagagct ttgattctca atcctaaact ttttttattg
      661 gacgagcctt ctcttggcct ttcgcccaat aatgtaaaaa ttgtattcga gaaaataaga
      721 gaaattaata aaaaaggaac aactattctt gttgtggaac aaaatgctcg gatggctttg
      781 gaatacgccg atagaggcta tattttcgaa gtaggcggaa ttatcatgaa tgatacagca
      841 aagaatttac taaacaatga aaaagtaaaa aagtcatttt taggagaaat taattatttt
      901 ggctaattct taataccttc tctcaataat atttattttg atttttattt ttataacttt
      961 aaacaacaag attcccccta atttctattt cattaaagtt ttttcatttt aatccagagc
     1021 ttaatttttc cggaatttta aaaactgttt aatgctttca taatcttttt tcaaggcttc
     1081 acgggggatg gggtgtgaaa accatatcac taaacccgtg attcctccaa tgaaaaaggt
     1141 agcgacaaat tgaagtaacg aaaacgccat ggccgattct gaacttatac cccaatgagc
     1201 caacaatact accaacgata attccctggt cccaaaccca gcccatgtaa ttggtattag
     1261 caatacagca ctcgtgatga tgatgacccc ggccatttcc caaaatccaa tgtgaaaatt
     1321 caaggctttg gctatcaaat acgattgcag aaaatatatc atataggcaa aaacagtaag
     1381 aagccaattt ttcatggaat gaatgcctgt gataggataa aggcttccgt acaagaatga
     1441 tataaaatta tgtttttgat tcttgtttct gaaacaatag agcaaaaccc ataaaatcag
     1501 taagccaaag atgacaatca tcactgaaat ccatagaaag gtaatatttt cggtttggag
     1561 caacaaggcg tacgagagca aacctccacc cagtacgaag tacaaatcga agaatctgtc
     1621 ggcaattacg gtctggaaag ccttaaacag cccgccttcg gaaaggtcac gtatgtaata
     1681 aactcgtacg atttcaccaa aacgtccagg tgtaagcaaa ccaatagaat aagaagtgag
     1741 ataagattta taagtattcg tggtatcgta atgtatgcct tctgttttca ttaaataatg
     1801 ccatctccgg ctttttaacc ataaaacgat gagtacaccc ggcaaagcca gtaaatacaa
     1861 ccataggtta atgcctctca taatgtcaac aaattcatta aaagtaaatc ccgtcctttt
     1921 aataatcaac cagattaaac caaccgctat aatggctttc aagagttgtg agaggatgtt
     1981 tttaaaagat ttcatcgttc ggttttcaaa gtttttccct ttttgtggaa taagttgctc
     2041 ttttccagta ttttctggca ttttcgaaaa tataacccat cgttttttga ggaatatggt
     2101 cagcaatata acgtgcaggt tttgtcccta aaaggaggaa aagaaaatcg atgagaattt
     2161 ccatcagata tcgacccagg ctaaatccgg aaattttata tccatagtcg ggatttttta
     2221 aacccaatac acttctgaat tgcatgcgga taaaggttcc tcgttttttc aaatcatatc
     2281 catgagagtg cattttgatg gcttcttctt cggcgatggg ccaaagtgtg atgatgtgtt
     2341 gatttgccat ctcctcgagt aaattttgtc cttgtttgct tcgagctata accaacgaaa
     2401 agccttttcc tctttcttcg taaacaggtg cccacgcatc acctcccgaa atatcggtaa
     2461 actcattggt aagatcagta cataatagac tgtttttaag aatatggaaa gggatgagat
     2521 agttggcatg aaactttggt aattccatta tgcgaccgtt tttcatttct atgcgcatgc
     2581 ggccgggcca ctcaccataa cggaaataga ttttttctat agatgtcaaa tctttttcct
     2641 tgtgtgcagc aagaaagctg cggatggagg aaacatgcag tgtgttgccg taaaatgggc
     2701 caaatatgta aagaattttg tcaactgaag gatgcttcat tttttgcagt ttccttatag
     2761 cttgaacctg gccgggtaaa cctacataag ccagtttgcc atcaaatttg gctatttgtg
     2821 gtaatatttc atttaccgaa ctaataacat acttgctttg agccgattcg agaatttctt
     2881 cttttgttgt ggcaataaat ggttcggtca gccatggttt ctccttggac attctcagag
     2941 taaccgctcc ctgtatttct cctttttcga gcaaatatat caacagggcg cttaaaattc
     3001 ctccactacc tgcattcgat ctgactattt ggtcatttgc atggccgatg tataaatttt
     3061 cgtaacttcc catcaaacgg ttgaaatgcg aagccgatgc gaacagccat tggttgaatt
     3121 cagggaaatg gaattcatga cccgagcaag cgctccagat ggttgcggct tcttcttgcg
     3181 taagaggaga aataattttt ggccgatatt tcccttccct atcctgaaaa aggatttttc
     3241 cattgctaag tcccacacag gttccacagc ggttgcacaa attcgcgcgc atgatgcact
     3301 ccagatgttt agatgcgttt cccaacataa aattgcctga ttcctaactc tcgaataatt
     3361 gaatgttgaa attttcgaat aagaaaatcg ccgagtttct gcagaaatac tggccctgca
     3421 ggaatgagca aagtgccttc gtgatggaga acttgccaac ccgtatcctt caacagcatt
     3481 tttacttttt tggatggcaa aaaacgatgt ggaccttcgc catgaccgcc aaaaagaaag
     3541 gtaaaaatac gataaggaaa ttcacttgta gctggcggac agctcaaaat aagaatgcct
     3601 gagggacgaa tgactctatg cagttctttc agaaaagcta cgggttcagc cacgtgttca
     3661 agggtttcga gacttacgat agcgtcgaat tcttcatcga caaagggtaa actgctgtaa
     3721 gtcgaaattt ttatctgttc gatatctggc cacagtctga aagcctcatt catcaaacct
     3781 tgcgatatct ctgcattgat cacccgaatg cccggataag tacgttgcaa gaaaatgctg
     3841 gcttctgcgt ctcgactggt aatgttcagg atttttgccc ccgatggtaa gggagggaac
     3901 aattcgagca tttttcgaaa cctctggtcg tgtgcatatt ttaccttgtt gttttccttt
     3961 acatacttat gtgccacttt atcccaatgc tcttcgacct gtttatcggt ccacttgttt
     4021 tttggcagtt tatacttcat gatttaatat ttccttcggt tcattaccga gatttgatcg
     4081 gccagtaaac caatcagaaa cagaaacatg ctgctaagga tgagaatagt ggctgtgttt
     4141 ggaaaccggc tgaaaacaac atagccatgg ataccccatc caagacctgc tataaaagct
     4201 ataacactaa tagggaaaaa tatttttaaa ggattaaaca gcatggtcat gcggaaaagt
     4261 aataaaaagg tcttggggcc gtcttttaga aattttacag tactgtttct gccttgacgt
     4321 tcttttacgt ggatggggaa agtgccaata gaataaccct ctttgaggaa agccagtgta
     4381 gaagtggtag aaaatgaaaa accattgggc atgatatgta gtaacgacag gatgagctgg
     4441 cgatcgaaac cacggaatcc ggagttgaaa tcgggtaatt tttgctcaac taaatattca
     4501 cctacaatac gtatgatttt ttttccagat gtacgagctt taacctgata ggaatcagaa
     4561 gttcgttccc ctataaccat gtcgtaatcc tgcaacattt gggcaatttt gggaaggaac
     4621 gcaggatcgt gctgtccatc gctatccata aagattactt tttcgccttt ggctttgcgg
     4681 attcctgttt tcaaggctgc cccataacct ttgttggttt catgcttgag cagacgtaca
     4741 ggatactgct gagctacaaa tgtagtttga tcagtagagc catcatccac cacaatgaac
     4801 tcaaaccgat cgaacagatg aagtgagttt attttgtcga gagtatttct gaggccttct
     4861 tcctcattat aagccgggat gataaccgaa atgaaaggag tattgctcat gcaaatgaat
     4921 tcgttttggc aaagatactg tctccatgtg gaatgagaaa atatgcttc
//
LOCUS       DAOORU010000165         4946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_20420_length_4946_cov_4.853200, whole genome shotgun sequence.
ACCESSION   DAOORU010000165 DAOORU010000000
VERSION     DAOORU010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4946
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20420_length_4946_cov_4.853200"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(533..>4946)
                     /locus_tag="PLF20_09100"
     CDS             complement(533..>4946)
                     /locus_tag="PLF20_09100"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HOX81193.1"
                     /translation="TITYSFTDPNGCTNTYSQKTTVLPAPAVDFSGLDPNHCLNSPIA
                     LLTGNFAPFGSFLGPGITDNLNGTAIFSAATAGAGGPYNITYTYQALNGCINHMTKQT
                     TVNALPVVSFSGLVTSYCLNSTPATLTGNQAPQGHFEGPGITDYGNGTAMFNPALAGV
                     GIHSIYYYFTDGNFCTDTMFKEVIVNALPQPSITNILSQYCVNASPDTITGNFYPLGS
                     FSGPGIIDLNIGKAIFDPDAAGVGGPYDIIYSYTDFNGCTGDTTYQITVLPKPSVDFN
                     PFSASYCIDDVPVLLSGNHLPYGSFSGPGIFDQGNGTAIFDPLAAGPGFKQVNYIFTD
                     NNGCYNDTTKTTKVIPFPEKPIAVTSTLNNFCTGWVTQINLGVIGGNGDTVEVYTENC
                     GGVLAGMFEPPSLLTVTAPTDTTTYYARWVNACGNSECDSIVINVIPFPLPPDSLGVD
                     TNNYCAGTVNIITLSAGGGIGSTLKWWKDSCGGTLVGIGQPLIITAPNDTTTYWATYE
                     NQCGISTCDSLQINVTPQPLSPSALQVDTNFFCTGTINQINLTATGGAGDTLYWFKDE
                     CGGNPIGYGTPLTISAPDSSAIYHARWGNKCNLSNCLSIEVEVLQPPVRPFSLSTNEN
                     DFCAEEVGVIQLSASGGSGDTLQWFKGNCGGIYIGTGSPLLISAPGDTTIYYSRWTNL
                     CGQSECDSIIINVRPQPRPLDSLSVDTNYFCISYNENIQLSAYGIYEYGDTVRWYEGG
                     CGQNFIGSGNSLTIAAPDVSTTYYANNNNSCGESICRSIQVIVNTPEPPVLLTANPLF
                     KCVSDTGQVTLKSFGGNGEIISWYADSCGGVPIGHGDSITISYPDTTTFFYARWENYC
                     GESPCVSIPVNVVEIPSPPAQILSDTNYYCIGDVDYITLTAFGGYGDTLSPWGETLRW
                     FVKSCGGATIGTGKNITIPAPVASTWFFARWENACGVSICDSFNLIVYTPRPPDSVIA
                     DTNYFCPGTVDSVSLMAWGGVGDQILWAKIYGNDTIAAGSTNPFRVAAPNTTTRYLVR
                     MKNYCGISKWFPFDIVVNLPQPPKLIVSDLDSLVCSNYPGYIHLWTIGGWGDTVKWYS
                     TDASGQNPVFLYSGDTLSLSPPTGTSYLMATFVTACGESKGTKTHIRTVPAPIVNIIS
                     NDSICEGSRYHVMGDLISNFHHLKWTVDGIGNLDHPTQKSPVYSYGNHDIINPTVVYL
                     KVNVFGNYPCGMVKDSLKLTINPKPELVLTPPSPALCRDSVLILSASGSETFKWTPNQ
                     DIDTTYGSLVKISPKHDMDYSLIGMNHSGCRDTLTIHVTVKPAPYLNLGEDLYLFSCD
                     PIILDAGGGDGSDFYEWQDGSTKRTLKVYGNGTYWVRVFNEGCAVVDTIKVQLCEGFI
                     YVPDAFTPNNDGLNDMFKVITTDPTIHFHIYIYNRHGQLVFQTDDIEKGWDGKIDGEM
                     APADVYVYLIEYRGNGTHSPGVTKTLKGSFILVR"
BASE COUNT         1503 a          998 c         1058 g         1387 t
ORIGIN      
        1 tagaacggaa gtttcctaaa ctttaaatcc aggttcgatt cctggtgggg ctacaaacat
       61 cgtcaactcc ttaaccttcc atatatttat taataaatag agtttttcgc tgaagattaa
      121 attttccaaa aaattctttc ttttatttgc cgaaaaggga aaggttgtat tatattttat
      181 tctcaggtga aaattatttt ttagatattc aaaaaattac agataaggaa taatattttt
      241 gaaaaagtct ttgagaagca actcgtcgat agaagttttt atcgaaattt atttccttaa
      301 tgttttcttt gttggattca aactttttgt agtattttta aagtaggcat gtttacttgt
      361 tctccttaga gaggaggaat agccatcatt gttattttcg tgagcttgtt gagaatgttg
      421 aaatttttat taacaattag caattaaaaa ttggataata gattgaaagc ctcaagaatc
      481 tataataacg aaaacatcat tagcaatacg gggagaagag acatcaaagt ttctatctta
      541 caagaatgaa gctccctttt agggttttgg tcactcctgg cgaatgtgtc ccattgccac
      601 gatattcgat cagataaacg taaacgtcgg ctggagccat ttctccatca attttaccat
      661 cccaaccttt ttcgatatca tctgtctgaa ataccaattg accatggcga ttatagatat
      721 aaatgtggaa atgaatcgta ggatcggtgg taattacctt aaacatatcg ttcaaaccgt
      781 cattatttgg tgtaaaggcg tcgggcacat aaataaaacc ctcacagagc tgtactttta
      841 tggtgtctac aactgcacag ccttcattga atacacgtac ccaatatgtt ccatttccat
      901 acacttttaa ggtgcgctta gtagaaccgt cctgccattc ataaaaatca ctcccatctc
      961 ctccgcctgc atccaaaata ataggatcgc atgagaatag atagaggtct tctcccagat
     1021 tcagatatgg cgcaggtttt acggtaacat gaatagtgag tgtatcacga cagcctgaat
     1081 gattcatccc aatcagagaa taatccatat catgcttcgg tgaaatcttg acaagagagc
     1141 cataagttgt atcaatgtcc tggtttggcg tccatttgaa cgtctcggac cccgaagcac
     1201 tcaaaatcag tacagaatct cggcaaaggg ctggtgaagg cggagtcaga accagttcag
     1261 gtttgggatt gatggtaagt ttcaaactgt cctttaccat tccacatgga taatttccaa
     1321 aaacattcac cttcagataa accacggttg gatttataat atcgtgattt ccatatgaat
     1381 aaactggaga tttctgcgtt ggatggtcaa gattaccaat gccatccact gtccatttta
     1441 aatggtggaa gtttgatata agatcaccca taacatgata gcggcttcct tcgcagatgg
     1501 aatcgttgga tataatattt acgatgggtg caggtactgt tcgtatatgt gttttagtac
     1561 ctttactttc gccgcatgct gttacaaatg ttgccatcaa gtaggaggta ccagtgggag
     1621 gtgaaagact aagagtgtct ccactgtata gaaatactgg attttgtcca gaggcatcgg
     1681 tggaatacca tttgacggta tcgccccatc caccaatcgt ccataaatga atatatccag
     1741 gataattgga acaaaccaga ctgtctaaat cactaacaat cagtttggga ggttggggga
     1801 ggtttacgac gatatcgaaa ggaaaccatt tcgaaatgcc acaataattc ttcatacgaa
     1861 ccagatagcg cgtggtagta ttaggagctg ctacacgaaa tggatttgtc gaacctgcgg
     1921 ctatggtatc attcccatat atttttgccc ataaaatttg gtcacctact cctccccatg
     1981 ccattaacga aacactgtcc acagtgccag ggcagaagta attcgtatcg gcaattacgg
     2041 aatcaggtgg tcgaggagta tatacgatta aattgaaaga atcacaaata gaaactccac
     2101 aggcattttc ccagcgagca aaaaaccaag ttgaagccac aggcgcagga attgtgatat
     2161 tttttcccgt acctattgta gctcctccac aggacttgac aaaccagcgc aatgtttcac
     2221 cccagggcga aagagtgtcg ccataacctc caaaagccgt aagtgtgata taatccacat
     2281 cccctataca atagtagttg gtatcggaaa gaatttgagc aggaggggag ggaatttcga
     2341 ctacattcac tggaatgctc acgcaaggcg attctccaca gtaattttcc catcgggcat
     2401 aaaaaaatgt agttgtatcg ggatatgaaa tagtgatcga atcgccatgg ccaattggca
     2461 caccaccaca tgaatcggca taccatgaaa tgatttcgcc attgcctccg aatgatttca
     2521 atgttacctg tccggtgtct gaaacgcatt tgaataaagg atttgcagta agcagtacag
     2581 gaggttcagg agtattgact attacttgaa tcgagcggca tatcgattct ccgcatgaat
     2641 tgttgttgtt ggcataatag gttgtgctaa cgtcaggtgc agcaatggta agtgaattac
     2701 ctgaaccaat gaaattttgg ccgcaacctc cttcatacca tcgaaccgta tccccgtatt
     2761 cgtatattcc ataagctgat agctggatgt tttcattata agagatgcag aagtagtttg
     2821 tatctacaga taatgaatcg agaggacggg gttgtggtct aacattgatg atgatagaat
     2881 cacattcgga ttgcccacaa agattagtcc atctcgaata atatatcgtg gtgtcgcctg
     2941 gagcgctaat caacaatgga gaacctgttc caatataaat tccgccgcaa tttcctttga
     3001 accactgaag agtgtctcca gaacctccac ttgccgaaag ttgtattact ccaacttctt
     3061 ctgcacaaaa atcgttttca tttgtactca ggctaaatgg ccgaactggc ggctgcaaca
     3121 cttcgacttc aatcgagagg caattgctca ggttgcattt gttcccccag cgagcatgat
     3181 agattgcaga agaatcgggg gctgaaatag tcaaaggagt tccatatcct atcggattcc
     3241 ctccgcattc atccttaaac caatataagg tatccccagc tcctcctgta gctgtaaggt
     3301 tgatctggtt tatggttcca gtacagaaaa aattagtatc tacttgtagg gcagaaggtg
     3361 ataaaggctg aggggttaca tttatctgga gactatcaca ggtagagatt ccgcattgat
     3421 tttcgtaagt agcccaatat gtggtggtat cgttgggagc cgtaataatt aaaggctgac
     3481 caataccaac caaggtgcct ccacacgaat ctttccacca tttcaaagta gaacctatgc
     3541 caccgccagc actaagagta attatattaa ccgtaccagc acagtaatta ttagtatcca
     3601 cacctaaaga atcggggggc aatggaaaag gaatgacatt gattacaatg gaatcgcatt
     3661 ctgagtttcc acaggcattc acccaccgag cataataagt tgtggtatcg gttggagccg
     3721 taaccgtcaa gagcgaaggc ggttcgaaca ttcctgcaag cactccacca cagttctctg
     3781 tataaacttc cacagtatcc ccgtttcctc caatcacccc aagatttatc tgagtgaccc
     3841 atcctgtgca aaaattattg agtgtacttg tcacagcaat gggtttttct gggaaaggaa
     3901 tcacctttgt ggttttggta gtatcattgt aacatccatt attatcggta aatatataat
     3961 tgacttgttt gaatccggga cctgccgcta aaggatcgaa aatggcggtt ccatttcctt
     4021 gatcgaagat tccagggccg gaaaagctgc cgtagggcaa atgatttccc gacaataaca
     4081 ccgggacatc atcgatgcaa taggaagccg aaaatggatt aaagtcgacg gaaggcttgg
     4141 gcagcacggt aatctggtaa gttgtatcgc cagtgcaccc attgaaatcg gtataagaat
     4201 atataatatc gtaagggccg ccaacgcctg cagcatcagg atcaaaaatt gcctttccaa
     4261 tattcagatc gatgatgcca ggaccactaa aagaacccaa cggataaaaa ttccctgtaa
     4321 tggtatcggg ggatgcattt acgcaatatt gagataatat attggtaata gaaggttgag
     4381 gaagagcatt cacgatcact tctttgaaca tggtatcagt acagaaattt ccgtcagtaa
     4441 aataataata tatggagtgt atacccacgc cggccagggc tggattgaac atggcagtac
     4501 catttccata atcggttatt ccagggcctt cgaagtgacc ttgaggagct tgattaccgg
     4561 taagtgttgc tggggttgag ttaaggcaat aagaggttac aagtccacta aaactaacca
     4621 cagggagagc attcaccgta gtttgtttag tcatgtggtt tatacaacca ttcaatgcct
     4681 gataagtata ggtaatattg taaggaccac ctgcacctgc tgtagcggct gagaaaatgg
     4741 cagttccatt cagattgtcg gtaataccag gccccaagaa tgatccgaaa ggagcaaaat
     4801 taccagtgag taaggcaatg ggagaattca ggcaatgatt gggatcgagg cctgaaaaat
     4861 cgactgcggg tgctggcaaa actgttgttt tttgtgaata agtatttgta cagccattgg
     4921 gatcggtaaa agaataggta atagta
//
LOCUS       DAOORU010000166         4847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_20930_length_4847_cov_4.968489, whole genome shotgun sequence.
ACCESSION   DAOORU010000166 DAOORU010000000
VERSION     DAOORU010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20930_length_4847_cov_4.968489"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..2296
                     /locus_tag="PLF20_09105"
     CDS             254..2296
                     /locus_tag="PLF20_09105"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOX81194.1"
                     /translation="MRKQLLLFMVLLSACMVGFPQNSPSKFGKVSMDELANKVCPIDS
                     NAHAYFIFNCGLSHFTYPQTVTREDDATESKGFILETSRHFRIKILDETAFDWANVEI
                     PLYVLGNDKEKIVSFKACTYNLENGKMEKTNFSKGNLIYEKTSDRWETAKAPLPNVKK
                     GSIIEVSYTIVSDFYFNLDKWLFQYSIPVLYSNYEVRIPEYFMYHTNIVGYYPVQVIN
                     SHKYESLTLTYIRGQLNGGDRITNTFNYEVGYNEYESTNIPAFMEDSYLKSSKNYLTA
                     VEFELSATKFPNSPMKSYSNTWEDVINQFLSNSEFGTRVKRTGFLDDVVQQISNKAES
                     QLEKMNMAFRHVANRYKWNEKSSVVTSKSLNNSYREGFGNSADINLCLVGLLQSMDIE
                     AYPMLLSTQSNGFVKLTHPSISNFNYVIALAKIDRKTYLLDATEPLTVVNQLPVRCLN
                     DKGLVVKSGPVEWIDLTLNTNYKSQVTLEYELAEDLQITGRYLSTLEGYAALEQRKEI
                     ISYGSQNDYIQSVSKNIPGIEINQFEIGGMDTLEHPVHLAFDFNYPNQVEEAGDLIIF
                     KPLLFQAIKRSPFKLKERIYPVEYNYHTADLVICQIKIPHGYSIESLPPSINMSLPDK
                     SARYVYTVMENNGKIVITAFFTRNKLTYLPKEYGDLKEFYRMMVDKQNEKIVFKKI"
     gene            2299..4209
                     /locus_tag="PLF20_09110"
     CDS             2299..4209
                     /locus_tag="PLF20_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOX81195.1"
                     /translation="MRRCILLFGIILSFHSYAQNHFNVVHIPDSLRENADAVIRYSNT
                     NYEIKNNVLLKQVSFAITILNENAKERASLVIPYNSYTNIKSVAGVVYNSMGIKTNKL
                     SKDDMADYAAYPSFTLFSDSRIKIARPVIKNYPITFEYIYEIEYSGFLMPESWMPILD
                     YRISLEKGHLQILIPDTIILFRYQNIQPAFNIKKLNGKFLYTWDVDYIKAFEKEPFSK
                     PFSMYMPKIFINIRNTGFYGHKVGYDNWESYGKMIYNLLIDRDKLPRETIEEISALCR
                     NAENDLGKVNLVYSYMQNKVRYVNVTKGIGGYQPIAAADVNKYGYGDCKALANYTRAL
                     LQSVGIKSYYTEIGSGKDKTIAFTDFASIDQTNHAILCVPLTSDTMWLECTNSNMPLG
                     YIGAANSNRKALLVAEEGGKLVNTPTYDEFANRNNVTGQIYIDTTGVVKLELILSYAN
                     ELFDEIMPLMSLSKDEQEKYIIHSMPINGFNLRSYKFENKSTLNYPLGELEIKAEIPK
                     YADLMGKRIAFKPNVINRYNFTLHKKRRTNFYISQSYTQKDSIIIHIPDGYRLEFEFQ
                     PVEFSCQVGSYNMMFTQIANHSFLMKRTLVLKRGEYSIDEYPAIYDFFRKIASYDNKN
                     CVFNKYNRINKN"
     gene            complement(4661..>4847)
                     /locus_tag="PLF20_09115"
     CDS             complement(4661..>4847)
                     /locus_tag="PLF20_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81196.1"
                     /translation="VIFSKADLIPDEVNKTLTIKLYSLATKRDNLAVQKDCDLLNDTE
                     IIFPGTNLTLVFKTATT"
BASE COUNT         1656 a          765 c          871 g         1555 t
ORIGIN      
        1 tgaagttgca tatctaaaaa tttttgcccg aaaatacgac aaattgaaat acggtggtac
       61 ttttgtaaaa tattgatatg caattatagg atcaggagtt ctgaaattaa gtttcctatt
      121 tttatatgat cgtttctggt tttcttctat ttgacaatct aaaatttttt aattttgtac
      181 cgttgttatt gtgtttaata ttgcctctaa catcattaac atattgatac ataatgcaat
      241 agatataaaa atcatgagaa aacaattgtt actttttatg gtcttattaa gcgcctgtat
      301 ggttggtttt cctcaaaact cgccttcaaa atttggtaaa gttagtatgg atgaattggc
      361 aaataaagtg tgtccgattg attcgaatgc tcatgcctat tttatattca actgcgggtt
      421 atcgcatttt acttatcctc aaaccgttac acgcgaggat gatgctaccg aaagtaaagg
      481 atttattctg gaaacctcga ggcatttcag aatcaagata cttgatgaaa ctgctttcga
      541 ttgggcaaac gtggaaatac cactctatgt tttgggcaat gataaagaaa agatagtttc
      601 ttttaaagcc tgcacttaca atttggaaaa tggaaagatg gaaaaaacaa attttagtaa
      661 gggaaatctg atttacgaaa aaacctcaga tcgttgggaa actgccaagg cacctttgcc
      721 taatgtgaag aagggaagca taatcgaggt ttcatatacc attgtgtccg atttttactt
      781 caacctcgat aagtggcttt ttcagtattc tattccagta ctctacagca attacgaagt
      841 acgaatccct gagtatttta tgtatcatac caatatagtt ggctattatc ctgttcaagt
      901 aataaatagt cataaatacg aatctctaac gcttacttac attagaggcc agctcaatgg
      961 gggcgataga attaccaata cattcaacta cgaagtgggg tataatgaat atgaaagcac
     1021 gaatattccg gctttcatgg aagatagtta tttaaaaagt tcaaaaaatt atcttactgc
     1081 cgtggaattc gaactttcag ctacaaaatt tcctaattca cctatgaaga gttattccaa
     1141 cacatgggaa gatgtaatca atcaattctt aagtaacagt gagtttggaa cgcgagtaaa
     1201 acgtactgga tttctagacg atgtggtaca gcaaatcagt aataaagcag aaagtcaact
     1261 cgaaaaaatg aatatggcat tccggcatgt tgctaatcgt tataaatgga atgagaaaag
     1321 cagtgtggtt acttccaaaa gtctgaataa ttcttataga gaaggttttg gtaactcggc
     1381 cgacatcaat ctttgccttg ttggactttt acaaagtatg gatatagagg cttatcctat
     1441 gttattgtcg acacaaagca atggatttgt gaaattaacg catccttcca tttccaactt
     1501 caactatgtg attgctttag caaaaattga tagaaagact tatttacttg atgccactga
     1561 acctctgact gtagttaatc agttgcctgt tcgttgccta aacgataagg gattggtagt
     1621 gaaaagtggt cctgtagaat ggatcgatct taccttaaat acaaattaca aaagccaagt
     1681 tactttagag tatgaattgg cagaagattt acagataact ggaagatatt taagcacttt
     1741 ggaagggtat gctgctttgg aacagcgtaa agagataata tcctatggtt cacagaacga
     1801 ttatatacaa agcgtttcga aaaatattcc agggatcgaa atcaaccaat tcgaaatcgg
     1861 tggtatggat acgctcgagc atcctgttca tttggcattc gacttcaatt atcctaatca
     1921 ggttgaagaa gctggtgatt taatcatttt caaaccattg ttatttcaag ccatcaaacg
     1981 atctcctttc aaactcaaag agagaatcta tcccgtcgaa tataattatc atactgcaga
     2041 tttagtaata tgccaaataa aaattcctca tggttattcc atagaatctc ttcccccgtc
     2101 tattaatatg agtttgccgg ataaatcggc ccgttacgtg tacacggtga tggaaaataa
     2161 tggaaaaatt gtgatcactg ccttttttac acgaaacaaa ttgacatatt tacccaaaga
     2221 atatggcgat ttgaaggaat tttacagaat gatggtcgat aaacaaaatg aaaagatcgt
     2281 ctttaaaaag atttaattat gcgtcgttgt attttactct ttggaattat tttgtctttt
     2341 catagctatg cgcaaaatca ttttaatgta gttcatatac ccgattcact tagggaaaat
     2401 gccgatgctg tcattagata cagtaacacc aactacgaga ttaaaaacaa tgtcttattg
     2461 aaacaagttt cgtttgctat cactattttg aatgaaaatg caaaagaaag agcaagctta
     2521 gtaattcctt ataacagtta tacgaatata aagagtgtag caggcgtggt ttataattcg
     2581 atgggaatta aaacaaacaa gctttctaag gatgatatgg ctgattatgc agcctaccct
     2641 tcattcacac tttttagtga tagtagaatt aaaatagcaa ggccggtgat aaaaaattat
     2701 ccaattactt ttgagtatat ttacgaaatc gagtattcag gattcttaat gcccgaaagc
     2761 tggatgccta ttcttgatta cagaatttcg ctggaaaaag ggcatttgca gattctaatt
     2821 cctgacacca ttattttgtt tcgatatcag aacattcaac cagccttcaa tataaagaaa
     2881 ttgaacggaa aatttttata tacctgggat gttgactata taaaggcatt cgagaaagaa
     2941 cctttttcga agccattttc gatgtacatg ccaaaaattt ttataaatat caggaacact
     3001 ggtttttatg gacataaagt tggttatgac aattgggaat cgtatggcaa aatgatttat
     3061 aatcttttga tcgatagaga caagttgcct cgtgaaacca tagaggagat tagtgcttta
     3121 tgcagaaatg ccgagaacga cttgggaaag gttaatcttg tttacagtta tatgcagaat
     3181 aaagtacgat atgtaaatgt tactaaagga attggaggct accaacccat tgcagcggca
     3241 gacgtcaata aatatggcta tggcgattgc aaagctttgg ctaactatac aagggcttta
     3301 ctacaatctg ttggaattaa atcctattat accgaaattg gaagtggtaa agacaaaacc
     3361 attgcattta ccgactttgc cagtattgac caaaccaatc atgcaatcct ttgtgtacct
     3421 ttaacttccg atactatgtg gttggaatgt acaaatagca atatgcccct gggctatatt
     3481 ggtgctgcta atagcaatcg aaaagctttg ctggttgccg aggaaggtgg aaaactggta
     3541 aacaccccaa cttatgacga atttgcaaat cggaataatg ttactggaca aatttatatt
     3601 gataccactg gagtggtgaa attagaacta attctatcct atgccaatga acttttcgat
     3661 gaaataatgc cattgatgag cttgtcgaag gatgagcagg aaaagtacat cattcattct
     3721 atgcccatca atggatttaa ccttagaagt tacaagtttg agaataaaag tacattaaac
     3781 tatccattag gagagctcga gattaaagcc gaaattccta aatatgccga tttaatgggc
     3841 aaaagaattg cttttaaacc taatgttatc aaccgctata atttcacttt gcataaaaaa
     3901 cgaagaacta atttctatat ttcacaatct tatacacaaa aagattcgat catcattcat
     3961 attccagacg gctaccgatt agaattcgaa ttccagccgg ttgagttttc gtgtcaggtg
     4021 ggaagttaca atatgatgtt cacacaaatt gccaatcata gtttcttaat gaagagaaca
     4081 ttagtgctca aaagagggga gtattccatt gatgaatacc cagctatcta tgatttcttc
     4141 agaaaaatag cctcctacga taataaaaat tgcgtcttta ataaatataa taggataaat
     4201 aaaaattaat ctcttcattc tcaaaatttt ctatacctta gaagatgaat tttacttagg
     4261 agatttttta aaaaattgaa ttgaattcct aaaaatgcgg tataagtata aattaacagg
     4321 gatgaactat aaaaacattc acatagagta aaaaagttaa taataattca aattacaagg
     4381 aagagtttaa atagagtttg tctataaact caaattgtta ataacttttt gataactaat
     4441 taattaaaac tgttattggt attatttttt attctaattt tgttggaaat cattaaaaaa
     4501 ataatgccat gcgttttttt gaagatttta agattttatt cgaacaacct gattggttct
     4561 atgatcctta attatgtttt attgatacag tattagagat gaaaccttat ttgttaaaga
     4621 tttttgaaaa tgatattcag aactcctgat tcggtgttaa tcaggttgtc gcagttttga
     4681 agacaagagt caaatttgta ccagggaaaa tgatttctgt gtcgttcaat aaatcacagt
     4741 ctttttgaac tgccaggtta tctcttttag ttgccagaga ataaagttta atggttagag
     4801 ttttatttac ttcatcggga atcaaatctg ccttcgaaaa aattaca
//
LOCUS       DAOORU010000167         4842 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_20954_length_4842_cov_5.776896, whole genome shotgun sequence.
ACCESSION   DAOORU010000167 DAOORU010000000
VERSION     DAOORU010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20954_length_4842_cov_5.776896"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="PLF20_09120"
     CDS             <1..1758
                     /locus_tag="PLF20_09120"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOX81197.1"
                     /translation="AENINGMKSYYPSSAPQMPASYKVGAPAIFILINEIYTRGVPED
                     PDWVEIYNNSDVQMDISGYLIYDAGGQSGAKPKKAFPEGSVIPAHGFLVIVVDDASES
                     GFGLSSGGEEIWLENNKGAIIDDVTFPALEPTQSYGRFPDGSPNWQILSTITKGTSNS
                     PGIGPVANLKINEIYSTGDATTPDWVEIFNAGNQSVDLSGWKIYDNGGQTGTKPKKEF
                     PVGTSIPAGGFFVITVDDGSASGFGLSSNGEEIWLEKPDETIGDDVTFPALETGQSYG
                     RYPDGSDNFQVFYVVTPGTANDNSLPPAAVVLKINEIYSRGDAISPDWIEIYNAGTET
                     VDLTGWKIYDNGGQAGTKPKKEFPSGATIDAGGFYVIIVDDTATSGFGLSSSGETVWL
                     EKPNGTVADEVVFPALEVGQSYGRYPDGSDNLQVLYVITPGAPNDNSTPPASVILKIN
                     EIFSRGDDTNPDWIEIYNAATQDVDLTGWKIYDNGGQAGTKPKKEFPAGEVIPANGFY
                     VIITDDAAASAFGLSSNGETVWLENPEGTVVDEVTFPALDVTQSYGRKPDGSDNFFIF
                     TEITRGSSNNNASTLPKRK"
     gene            1786..2427
                     /locus_tag="PLF20_09125"
     CDS             1786..2427
                     /locus_tag="PLF20_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81198.1"
                     /translation="MAENLEIGKISKPRFEFRSFGQNFDNAAFRMSRLSVPVPENVWE
                     RHSDEIYIISRTNDVNNTKIRDGKMDIKTLVNISHGLEQWNPLMKGEFPMKTSSLLSE
                     VFMSFQLPLPNLNKEEYTFDEFLQIVYQHPDLGAVRVHKQRFGYMVNNTICEYAVVLI
                     NGARVVSVSSESTEIDDILKTISDLKLEGVENINYLQAIKRVIGMIHKPLANE"
     gene            2431..2898
                     /locus_tag="PLF20_09130"
     CDS             2431..2898
                     /locus_tag="PLF20_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOX81199.1"
                     /translation="MAQEIEKKFLVKGDFKPFAHQATRITQGYLSSVPERTVRVRIKG
                     NKGYITVKGIGNESGASRFEWEMEIPLEDAEQLMKICEPGVIDKTRYLIKAGQHTYEV
                     DEFYEENQGLVLAEVELQREDEPFEKPEWLGEEVTGDPRYFNSMLMKNPYSCW"
     gene            3018..4622
                     /locus_tag="PLF20_09135"
     CDS             3018..4622
                     /locus_tag="PLF20_09135"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HOX81200.1"
                     /translation="MDIIEQTFDPLDMNQYKIEKLPKREKNKTEKFLSFIGPPLALIV
                     FLLLAFVLKPSFLQSFDPEKLTKGAKEVFDRVGSKVFYRNNCYMLAIFAASLILWITG
                     AIPNYLTSLIVIISIVLTGVLPEKEAFAQLGHPVMWLNIMSFVLASMLVSTGVAKRFA
                     LWFLLKFGQSASGIFLSFLVINVVLSAFISATTAKAAILLPIFMVIAAIYGARGGDQR
                     NNFGRSIVLQNLLYINVGASGFLTGSGANLLAVSIIAGATGARIFFSDWLMAMFPMAI
                     GLMLIGYLLAMRVFFPLKPEERLPQIEGGMDRLREVYNELGPMSRKEKKAIILFITIL
                     AFWSTDRLHGISPTAVAFLGAIVALLPRIGILTWNEVDIPWHLMLFSAGAYTLGAGFS
                     ATDLPAISVNAFFDHLGMTQNTPFWALYLLLTGVMVYSALLFESKTMRAMIFVPIAIG
                     VATRFGYSIESLALPVAFMTEHVYVLPFNSKPAALLYETGHYSLTDTFRYGITMMTIA
                     WFLNIVAGETWFRLMGITPNGLFGLF"
BASE COUNT         1381 a          979 c         1095 g         1387 t
ORIGIN      
        1 gctgaaaaca tcaatggcat gaagtcgtat tatccttcga gtgcacctca aatgccagct
       61 tcgtataaag taggtgcacc ggctatattt attcttatca acgagatata tactcgtgga
      121 gtacctgaag atcccgattg ggtggagatc tacaataatt ctgatgttca gatggatatt
      181 agtggatatt tgatttatga tgcaggcggt cagtcgggag ccaaacccaa gaaggctttt
      241 cccgaaggtt ctgtcattcc ggctcatggt tttttggtaa ttgttgtgga tgatgcatcc
      301 gagagtggtt ttggcctaag tagcggaggc gaagaaatat ggctcgaaaa caataaagga
      361 gccatcatcg acgacgtaac cttcccagct ttagaaccca ctcaaagcta tggacgtttc
      421 ccagatggca gccccaactg gcagatactc agtactatta ccaaaggaac ctcaaactca
      481 ccaggtattg gaccggtagc gaatctaaaa atcaatgaga tttattctac gggtgatgcc
      541 actactcccg attgggtcga aattttcaat gccggcaatc aatctgttga ccttagtgga
      601 tggaaaattt atgataatgg cggccaaaca ggtacgaaac ccaaaaagga gtttcctgta
      661 ggcacaagta ttcctgcggg aggatttttt gtaattacgg tggacgatgg ctctgccagt
      721 ggttttggac tgagcagcaa tggagaagaa atttggctcg aaaaacccga tgaaaccata
      781 ggcgatgatg taactttccc ggctctcgaa accggacaat cttatggacg atatcccgat
      841 gggagcgata acttccaggt attttatgtg gttactcccg gaacagccaa cgataattct
      901 cttcctccag cagcagtagt attgaaaatt aatgaaatat attcacgtgg ggatgctata
      961 agccccgatt ggattgaaat ttataatgct ggaactgaaa ccgtcgattt gactggctgg
     1021 aaaatttacg ataatggagg tcaggctggg accaaaccca agaaggaatt tccatcggga
     1081 gcaactattg atgctggtgg attttatgta attatagtgg atgatacggc taccagtggc
     1141 tttggcttaa gcagtagcgg agaaacggtg tggctcgaaa aaccaaatgg cactgtggcg
     1201 gatgaagtag tcttcccagc ccttgaagta ggtcaatcgt atggccgata tcctgatgga
     1261 agcgataatc ttcaggtttt gtacgtaata acacctgggg ccccaaatga taattccact
     1321 cctccggctt ctgtgattct gaaaatcaat gagatatttt cgagaggtga tgatacgaat
     1381 cccgattgga tcgaaattta caatgcagca actcaggatg tagatcttac gggttggaaa
     1441 atatacgata atggcggaca ggcgggaacg aaacccaaga aagaatttcc tgcgggcgaa
     1501 gttatacctg cgaatgggtt ttatgtaatt ataacagatg atgctgctgc aagtgctttt
     1561 ggcctgagca gcaatggcga aactgtatgg ctcgaaaatc ccgaaggaac ggtggtagac
     1621 gaagttactt ttccagcact cgatgtaact caatcgtatg gcagaaaacc tgatggcagc
     1681 gataatttct tcatctttac cgaaattact cgtggcagct cgaataacaa tgcatcaaca
     1741 ttgccaaaac gtaaataata aactaaccga ctaaaatgaa tttctatggc tgaaaacctc
     1801 gaaatcggaa aaatatccaa accaagattt gaattccgca gttttggtca aaacttcgat
     1861 aatgctgcat ttcgcatgtc gcgtctgtcg gtacccgttc ccgaaaatgt ttgggaacga
     1921 cactccgacg aaatctacat catttcccgc accaacgatg tgaacaatac caagatccgc
     1981 gatggaaaaa tggatatcaa gactctggta aatatttcgc atggactcga acaatggaat
     2041 ccacttatga aaggcgagtt tcctatgaaa acttcatcac ttctttcgga ggtcttcatg
     2101 tcttttcagc tccctttgcc taatcttaac aaagaagaat atactttcga cgaatttttg
     2161 caaatcgttt accaacatcc tgatttaggt gcagtacgcg tgcataaaca acgtttcggt
     2221 tatatggtga acaatacgat ttgcgaatat gccgttgtac tcatcaacgg ggcaagagtc
     2281 gtaagcgtaa gttccgaatc caccgaaata gacgatatct tgaaaaccat tagcgattta
     2341 aaactcgaag gtgttgaaaa tatcaattat cttcaagcta tcaaacgggt aataggaatg
     2401 attcataaac cccttgcaaa cgaataaatt atggcacaag aaatcgaaaa aaaattcctt
     2461 gtaaaaggtg attttaaacc ctttgctcat caagctaccc gtatcactca gggttatctt
     2521 tcttcagtac ctgagcgaac agtacgagtg aggatcaaag gaaacaaagg ctatatcacc
     2581 gtaaaaggaa ttggcaatga atcaggagct agccgttttg agtgggaaat ggaaattcca
     2641 ctcgaggatg ccgagcaatt gatgaagata tgcgaacctg gggtaataga taaaacccgc
     2701 tatctaatca aggctgggca gcatacctat gaggtagatg agttttatga agaaaaccaa
     2761 ggattggttt tagctgaggt tgaattgcag cgtgaagacg aacccttcga aaagcccgaa
     2821 tggttaggcg aagaagtaac cggcgaccct cgttacttca attccatgct gatgaaaaat
     2881 ccttattcgt gctggtaaac cactccaccc tcgaagatcc agtccggaga ggaatgtctc
     2941 ttcggactgg atctttcact tttatctaag tttttaaaat gtattcattg attcgttgaa
     3001 ttcttaatca ctcagaaatg gatatcatag agcagacttt cgatccgctc gatatgaatc
     3061 agtataagat cgaaaaactc cccaaacgtg aaaaaaataa gaccgaaaaa tttttatcgt
     3121 ttataggtcc acctttggct ttgatcgttt tcctattatt ggctttcgtg ttgaagccct
     3181 catttcttca aagcttcgac ccggagaaac tcacaaaagg tgctaaggaa gttttcgata
     3241 gggtggggag taaggttttt tacaggaaca actgctatat gctggctatt tttgctgcca
     3301 gtcttatttt atggattact ggagctattc caaattacct tacttcgctc atagtcatca
     3361 tttctatagt tcttactggg gttttgcccg aaaaggaggc ttttgcgcag ttgggtcatc
     3421 ctgtgatgtg gctgaacatc atgtcgtttg tcttggccag tatgcttgta agtacggggg
     3481 ttgccaagag gtttgctctg tggtttctac tgaaatttgg acaaagtgca tcgggaattt
     3541 ttctgagttt tttggtaatc aacgtggttt tatcggcctt catatcagcc actacggcca
     3601 aggctgccat tttattgcct attttcatgg ttatagctgc aatttacgga gctcgaggag
     3661 gagatcagcg caataacttc ggccgaagta tagtgttgca aaacctctta tacattaatg
     3721 taggggctag tggatttctt acgggctcgg gagccaatct actggcagtt tctattattg
     3781 cgggtgcaac gggcgcaagg atatttttct ccgattggct tatggcgatg tttcccatgg
     3841 ccattggttt gatgctcata ggatatttat tggctatgag agtatttttc cccttgaaac
     3901 ccgaagaacg tctccctcaa atcgaaggtg ggatggatcg tttgcgagag gtttataatg
     3961 aacttggccc tatgagccga aaagaaaaaa aagctatcat tctctttata accattctgg
     4021 ctttttggtc aaccgatcgg ctgcatggga ttagccctac ggcagttgca tttttaggtg
     4081 ccatcgttgc tctattgccc cgtattggta tccttacatg gaatgaggtc gatattccct
     4141 ggcatctgat gcttttctcg gccggagctt atactcttgg agctggattt tctgctaccg
     4201 atcttcctgc cattagcgta aatgcttttt tcgatcatct gggcatgaca caaaatactc
     4261 ctttctgggc tctttatctt ttgctgacgg gcgttatggt atacagcgcc ctcttattcg
     4321 aatcaaaaac catgcgggct atgattttcg ttcctatcgc tatcggagta gctacccgct
     4381 tcggatacag catcgaaagt cttgcattgc cagtggcctt tatgactgaa catgtttatg
     4441 tactcccgtt taactcgaaa ccggcggcac tgctctatga aaccgggcat tacagcttga
     4501 ccgatacttt ccgctatggc attaccatga tgaccattgc atggtttctg aacatcgtgg
     4561 cgggcgaaac atggtttaga ttgatgggaa ttacaccgaa tggcctcttt ggattatttt
     4621 aatatagagt tctgatacaa cctcgagcta tcactatttt atcgttattt taagtacaaa
     4681 taattattta agaatgagat gatagaagaa caagtaaaaa ttcacgataa attctcggtc
     4741 gagataaagt tgcggctggc tgcaaggcgc aaagccaaaa agagtgaatt tgctgtaaac
     4801 acatggttat ttattcctgc atcacttgat attaatcact ct
//
LOCUS       DAOORU010000168         4835 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_20986_length_4835_cov_3.820084, whole genome shotgun sequence.
ACCESSION   DAOORU010000168 DAOORU010000000
VERSION     DAOORU010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20986_length_4835_cov_3.820084"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="PLF20_09140"
     CDS             complement(<1..993)
                     /locus_tag="PLF20_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81201.1"
                     /translation="MKTIGREFLRNIFIKSLAITIYSAGCFIPISVKAQCPPVITPQP
                     APGVIINGNIVRLCAGDSVILSSSPAAASGYLWYHNGTLIPDSIRSIFTAKVAGTYKA
                     KAPGCSVFSNTISLTVNPLPTAYITSNPIPPICSGTPITFVLHTNAPEYYWLPPVPSA
                     LMTEDTIMLELSSPTTMKAVAVTNTTPQCSYFTTYWVRVDFPINGGVIASDQTICSGT
                     VPEPFTNIEYPSGGNGTYTYKWVMSTSGPYGPWISIAGANDSIYTHPTPLTQTTWFAR
                     FAESPPCLEVISNPVMVEVKPIPEILAYSDAEICSGNIFSYFPINSDPGGTTDYT"
     gene            1832..2338
                     /locus_tag="PLF20_09145"
     CDS             1832..2338
                     /locus_tag="PLF20_09145"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOX81202.1"
                     /translation="MEPYIDANTMEIHSSKHHAAYTGNMNKAAKGQAFESVPLLEIFT
                     EVDKYPLALRNNGGGFYNHYLYWEIMKPQGGGLPSEMLQKAIQEDFQDFETFKAEFSK
                     AALDRFVSGWAWLSVDEFVHLFVSFTSNPDNSFMRVIEKCNIAILCFDGCEHVYYLNY
                     RNQDGYYV"
     gene            2722..3186
                     /gene="nuoE"
                     /locus_tag="PLF20_09150"
     CDS             2722..3186
                     /gene="nuoE"
                     /locus_tag="PLF20_09150"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOX81203.1"
                     /translation="MANTEMLVKELADKYGRNRESLLPILQELIDERRYVNDETMINV
                     ARELDLSAAEVYGTASFYSFIETEEVGKYVIRLCKTIVCDMKGKENVLQAIENTLKIK
                     VGQTTADKKFTLKTTNCLGWCHKGPAMLINDEVYTELTPGKAIDIIESYINK"
     gene            3224..4387
                     /locus_tag="PLF20_09155"
     CDS             3224..4387
                     /locus_tag="PLF20_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOX81204.1"
                     /translation="METKKLTRVDYIFRPDNTCLDTLKKSLRRDRDEIILELIDSGLK
                     GRGGAGFLTGLKWKLAKEAAGDVKYVICNADEGEPGTFKDREILDRVPRRVFTGMALC
                     GYVIGSKEGFLYLRAEYKFLAPKLMSIMAEMNQEFKKLGLDFTLNIRYGSGAYVCGEE
                     TALIDSMEGRRGEPRNKPPFPVTEGYLNKPTVVNNVETFVNCTIIMGIGAEEYKRFGT
                     DSSRGFKLFSVSGDCSKEGIYELELGMLLSDFVEEFGDGDTKAVQVGGASGFCVPRKK
                     FNQTIIGFEGIPTGGSMKIFNSSRSMYHVLHNYLEFFEEESCGQCTPCRVGCQQLLKG
                     IEAVKSKQRSSSYLDELVKLAKVMKVASKCGLGQSVGNAFVSIVDNFREEIIY"
     gene            4409..>4835
                     /locus_tag="PLF20_09160"
     CDS             4409..>4835
                     /locus_tag="PLF20_09160"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOX81205.1"
                     /translation="MINMINLTINNIPVSVEEGTTIIEAARRLSIRIPSLCNHDDLCV
                     SGNCRVCVVEQVGNPTLQAACATPVRENMQILTNSMMVMQARKTIIELLLSEHNADCT
                     KCYKNGNCELQDLSNEYRIGNQVYIDLIPLKHYTIDQSSP"
BASE COUNT         1498 a          843 c          987 g         1507 t
ORIGIN      
        1 ggtataatct gtcgtacctc caggatcact gttaatggga aagtaggaaa agatatttcc
       61 cgaacaaatt tctgcatcgg aataagccag aatctcagga ataggtttta cttctaccat
      121 tacagggtta cttatgactt ctaaacaagg agggctttca gcgaaacgtg caaaccatgt
      181 tgtttgagtt aaaggtgtag gatgtgtata aatactatca ttggctcccg ctatggagat
      241 ccagggccca taagggccag aagtgctcat aacccattta taagtgtatg ttccgtttcc
      301 tcctgaaggg tattctatat tggtgaatgg ttctggtact gtacctgaac aaattgtttg
      361 gtctgaagca ataacgccac cattaatcgg gaaatcaaca cgaacccaat aagttgtaaa
      421 ataagaacat tgtggtgtag tgttggttac tgcaacggct ttcatggttg ttggtgaaga
      481 taactcaagc ataatcgtgt cctctgtcat tagggcagaa ggtacgggtg gcagccagta
      541 gtattccggg gcattggtat gcaggacaaa agtgataggt gttccactac aaataggagg
      601 aatgggatta ctagtaatgt aagcagtagg taaaggattt acagtaagag aaatagtatt
      661 cgaaaaaaca ctgcaaccag gggcttttgc cttatatgta cctgctactt ttgccgtaaa
      721 tatacttctt attgaatcgg gaattaatgt tccattatga taccataaat atccacttgc
      781 tgcggcagga ctcgaggaaa gtataactga atcacccgca cataacctga caatgttgcc
      841 gttgatgata acccctggtg ctggctgagg cgttataaca ggtggacatt gtgccttgac
      901 tgatattgga atgaagcatc ctgcagaata aatagttata gctaagcttt ttatgaatat
      961 atttcgtaaa aactctctcc cgatagtttt catggttttg gtgatttgaa tggataaaag
     1021 tagtgttttc tttatttcat tgggcagcta ttggttttat tcttttcatt ttgaattttc
     1081 gcttcaaata taaaaatttc ttgctttttc tgtgccctct ttaatgaaaa tttatttcga
     1141 cctgttacat tcaaaagaaa ttttatttta aaatattgaa tatcaattta ttataatcaa
     1201 tttattttaa atagtaaaga aaatataata ctctagtact accttatctt tagtcatttt
     1261 attacaagag aaatttgaat aaaacaaact cattattttg aaccccttac ctgccatcaa
     1321 tatttaacag tctaattctt taaattattg ttttttattt agttatgcta agaagtttag
     1381 tatggcttct tttgctcttg tcagaaataa gaatctaatt ccaaatgcaa gatgaattct
     1441 gttttgtttt aaacttgata ttaccatcat tcccaatgaa taaagaagag atactaaaat
     1501 gtaaattatt gtttaataat atattaaagt atgcttttat tttacaaccc tgattatcga
     1561 aaacaaaaat gaattaatcg atctttaagt tttgtggtgt tctttcccta tttgaacaag
     1621 tcctgttatt tcttgttgaa actcaacaac aaaatcatat ctaaccatga ataaatggtt
     1681 acaatgtttt tcgcctaact tttttatctg ggagttttta aattgaatgt ttcggctgct
     1741 gatgggtcag ctattgctaa aaaattgggc gactcacagt taacggtcaa gagcacacaa
     1801 gagcctttat actatcccca actatactgc aatcgagcct tatattgatg ccaataccat
     1861 ggagattcat tcttccaaac atcatgcagc atataccggc aatatgaaca aagccgctaa
     1921 aggccaagcc tttgaatcgg ttcctttgtt ggaaattttt accgaagtgg ataaataccc
     1981 cttagcattg cgaaataatg gaggaggttt ttataatcat tatttatact gggagataat
     2041 gaagccccaa ggagggggtt tgccatctga aatgttgcaa aaagccattc aggaagattt
     2101 tcaagatttt gagaccttca aggcagaatt ttcaaaggct gccctcgacc gttttgtcag
     2161 tggatgggca tggctgtcgg ttgacgaatt cgttcatttg ttcgtttcat ttacttccaa
     2221 tccggataat tcattcatgc gcgttataga gaaatgcaac attgctattc tctgcttcga
     2281 tggatgtgaa cacgtctatt atctgaatta tcgaaaccaa gacggttatt atgtttagaa
     2341 tttctgcaac ctcatcagcc gacagaaatc aaaaaccaat attccgaagc tttaacagct
     2401 ttcgatatac gaaatcaagt cgattttgat ttgcctttgc gggtcattga acttggtgat
     2461 tgaattcgaa tttaagatat ctatcacgga ggtattcatt gaataattct tagacaaaag
     2521 gcataagaat ctggtaatga ataaaattta tttagactgt gtttaaatca tgtctttttt
     2581 gcgaaaaaca ttttttttcc attaagaaat tatgataatt ttgtcaacct attgttagca
     2641 cgctaacaat aggtttttcg ttttactatt aggcaatcaa tcaaaatttt agtgataact
     2701 cattttaaaa taggaggata aatggcgaat acagaaatgc ttgttaagga attagccgat
     2761 aaatacggcc gaaaccgcga atctctcttg cctatcttac aggaattgat agacgagagg
     2821 cgttatgtaa atgacgaaac catgatcaat gttgcacgcg aactcgattt atcggctgca
     2881 gaggtttatg gaaccgcttc tttctattct ttcatcgaaa ccgaagaagt tggtaaatat
     2941 gtaattcgat tgtgcaaaac aatagtttgc gacatgaaag gcaaggaaaa cgtacttcag
     3001 gcaatagaaa ataccctgaa gattaaagta gggcaaacca ctgccgataa gaagtttacc
     3061 ctgaaaacaa ccaattgcct cggatggtgc cataaaggcc ctgctatgct cattaatgat
     3121 gaagtttata ccgaattaac ccccggcaag gctatcgaca tcatcgaaag ttacataaac
     3181 aagtaaacca agatattttt tgacaggaaa aaaacaaaac gatatggaaa ccaagaaatt
     3241 gacacgtgtt gattatattt ttcgacccga caatacatgc ctggatactt taaaaaaatc
     3301 ccttcgacgt gatcgtgatg aaatcattct cgagttgatt gattctggtt tgaagggcag
     3361 aggcggagca ggattcttga ccggtttgaa atggaagctt gccaaggaag cagccggtga
     3421 tgtaaaatat gtaatttgca atgctgatga aggcgaaccg ggtactttca aagacaggga
     3481 gattctcgac agagtgcctc gacgtgtatt cacgggaatg gctctatgtg gttatgtaat
     3541 tggctccaag gaaggattct tgtatttgcg tgccgaatat aaattcctcg cgcctaaatt
     3601 aatgtctatt atggccgaaa tgaatcagga attcaagaaa ttggggctcg atttcaccct
     3661 caatattcga tatggcagtg gggcttatgt ttgtggcgaa gaaactgcat tgatcgattc
     3721 gatggaagga cgccgcggtg aaccacggaa taaacctcct tttccggtta cagaaggtta
     3781 tttgaataaa cccacggtgg taaacaatgt cgaaactttc gtcaattgta ccattattat
     3841 gggaataggt gccgaagaat acaagaggtt cgggactgat agttcgagag gcttcaagtt
     3901 gttttcggtg agtggtgatt gctccaaaga gggtatttat gaactggaat taggaatgct
     3961 tctcagcgac ttcgtagaag aatttggaga cggtgatacc aaagctgtgc aagtgggtgg
     4021 agcttctggt ttctgcgtgc ctcgtaagaa attcaatcaa accatcatcg gtttcgaagg
     4081 aattccaacg ggcggttcaa tgaagatttt caacagtagc cgttcaatgt atcacgtttt
     4141 gcataattat cttgaatttt tcgaagagga atcatgtgga caatgtactc cttgccgggt
     4201 tggctgccag cagttgctca aaggcattga agccgtaaag agcaaacaac gctcctcttc
     4261 ttaccttgat gaactcgtta aacttgcaaa agtgatgaaa gtggcatcga aatgtggcct
     4321 tggccaatct gtgggcaatg ctttcgtttc tattgtcgat aattttagag aagaaatcat
     4381 ttactaaaac ttttgaaagg aaaatgttat gataaatatg attaatctga ctattaacaa
     4441 tataccggtt agtgtagaag aaggaactac tattatagaa gctgcacgta gactgagcat
     4501 tcgtattcct tccctctgca atcatgatga tctttgtgtt tcaggtaatt gcagggtgtg
     4561 tgtcgtcgaa caagttggca accctacact tcaggcggct tgcgctactc ctgtaagaga
     4621 aaatatgcaa attcttacca atagcatgat ggtaatgcaa gcccggaaaa ccattatcga
     4681 actgttgctt tccgaacata atgccgactg taccaagtgc tataagaatg ggaattgcga
     4741 acttcaggat ttgtctaatg aatatcgtat aggcaatcag gtatatatcg atttgatccc
     4801 tttgaaacac tatacaattg atcaatcttc tcctt
//
LOCUS       DAOORU010000169         4698 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_21751_length_4698_cov_5.298729, whole genome shotgun sequence.
ACCESSION   DAOORU010000169 DAOORU010000000
VERSION     DAOORU010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21751_length_4698_cov_5.298729"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..3736)
                     /gene="nifJ"
                     /locus_tag="PLF20_09165"
     CDS             complement(200..3736)
                     /gene="nifJ"
                     /locus_tag="PLF20_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOX81206.1"
                     /translation="MNKKKNFITCDGNMAAAHVAYMFSEIAAIYPITPSSTMAEYVDE
                     WAAFGRKNIFGEPVKVVEMQSEAGAAGAVHGSLQSGALTTTFTASQGLLLMIPNMYKM
                     AGELLPAVFHVSARTIATHALSIFGDHSDVMATRQTGFALLASGSVQEIMDIAPVAHL
                     TTVKARIPFLHFFDGFRTSHEIQKIEVFGEAEVASLLDKQALAAFRSRALNPEHPVTR
                     GTAQNPDIFFQAREASNRFYDAVPDLVEHYMNEMTRITGREHHPFDYYGALDAENIII
                     AMGSVTETIKEVIDYKLKQGEKIGLICVHLYRPFSSKYFFKVLPSSVKRIAVLDRTKE
                     PGADGEPLYQDIRSMFYGMDNTPLIVGGRYGLSSKDTTPSQILAVYDNLRMPEPKNDF
                     TIGIVDDVTFKSLPLLPEQSFAPEGTYEAKFYGLGSDGTVGANKNTIKIIGEETDLYC
                     QAYFAYDSKKSGGFTASHLRFGKHPIRSTYLVTNPDFVACHVPAYLNRYDMLKGLKKG
                     GTFLFNSIWDVEETKRRLPDHIKKYMAENNISFYIINATAIAEEIGLGHRTNTIMQSA
                     FFKVSKVISYDLAVEKMKEMIKKSYGRQGEDIVRMNYAAVDRGGDVIKIEIPAEWANI
                     QIEVEKDERPLPEFIKKVVEPMNAQKGDDLPVSVFLDREDGTFPAGTTKYEKRGIAVT
                     VPEWIPENCIQCNQCAYICPHAAIRPFLLTAEEAASAPEGTTTLKAVGKGMGEMKYRL
                     QVSVLDCTGCGNCVEECPSKNKALIMKPLDNQHSEISRWEFMDTKIGYKEQYFDKTKN
                     VKNSQFAQPLFEFSGACSGCGETPYIKLITQLYGERMIIANATGCSSIYGGSAPSTPY
                     TSNYDSGHGVAWANSLFEDNAEYGFGIATGIQKLRNRIAMRMKEAMEKDISPELKEAY
                     GLWIEGMNDYDKSNEATKLMIPLLEKADDEISKEILALHQYLIKKSIWVIGGDGWAYD
                     IGYGGLDHVMASGEDVNVLVLDTEVYSNTGGQASKSTPTAAVAKFAASGKRVRKKDLG
                     AMAMTYGYVYVAQVAMGANQGQYLRAVREAEEYKGPSLIIAYSPCINHGLKAGMGKSQ
                     QQEKFAVECGYTALYRYDPRLETEGKNPFQLDSKEPDWSKFQEFLNSEVRFTSLKKAF
                     PKEAEELFAAAEENARWRYRSYLRMASLDYSI"
BASE COUNT         1290 a          985 c         1002 g         1421 t
ORIGIN      
        1 tgtcttcaaa actgcgacaa cctgattaac accgaatcag gagttctgaa tttaaaataa
       61 agcattccag accacaagag cgtatgtatt ttcttgttgt tcattttatt aaggaagcac
      121 cgattgcgaa attagcgaaa gaaacaaccc tgaaggtcaa cgccttcagg gttgtttctt
      181 atgattgaaa ctcaagatgc tatatactgt aatccaaact tgccatacga agataggagc
      241 gatatctcca gcgagcattt tcttcggctg cggcaaagag ttcctcggct tccttgggga
      301 aggctttttt tagggaagtg aagcggactt cgctgttaag gaattcctgg aatttactcc
      361 aatctggttc ttttgaatcg agttggaaag gatttttccc ttcggtttcg aggcggggat
      421 cataacgata cagagcagta tagccacatt ctaccgcaaa tttttcctgt tgttgcgatt
      481 ttcccattcc ggctttcaaa ccatgattga tacagggtga atatgcaata atcaaagaag
      541 gacctttgta ttcttcggct tctcgaactg ccctcaggta ttggccctgg ttggctccca
      601 tagctacttg tgccacataa acatagccat aagtcatagc cattgcgcca agatcctttt
      661 tgcgaactcg tttaccagaa gccgcaaatt tggctacagc tgctgtagga gtagatttcg
      721 atgcctgccc acccgtattg gaatacactt cggtgtcgag taccagcaca tttacatctt
      781 cacccgaggc cattacatga tccaatccac catagccaat gtcgtaagcc caaccatcgc
      841 ctccaatcac ccagatggat ttcttgatga gatattgatg gagtgccaga atttctttcg
      901 aaatctcgtc atcggccttt tccagcaaag gaatcataag ttttgtggct tcattcgact
      961 tatcataatc gttcatgcct tctatccata aaccataagc ctccttcagt tcgggactga
     1021 tatctttttc catggcttct ttcatcctca tggcaatccg gttgcgaagc ttctggattc
     1081 ctgtggcaat tccaaatcca tattctgcat tgtcttcgaa gagcgaatta gcccaggcaa
     1141 caccatgtcc tgaatcataa ttcgatgtgt aaggagtact tggagccgat cctccataaa
     1201 tagaagaaca ccctgtagca ttggcgatga tcattctttc gccataaagt tgagtaatca
     1261 gtttaatgta tggagtttcg ccacatcctg agcatgctcc tgagaattcg aataaaggtt
     1321 gggcaaactg gctgttcttt acgttcttgg ttttatcgaa atattgctct ttatatccaa
     1381 ttttggtatc catgaattcc cagcggctga tttctgaatg ctggttatcg agaggtttca
     1441 ttattagggc tttgttcttc gaaggacatt cttcgacgca attaccgcaa cccgtgcaat
     1501 cgagcacact cacctgaagg cggtatttca tttcacccat tcccttgcct acagccttaa
     1561 gggttgtggt tccttcgggt gcacttgcag cttcttcagc agtcaataaa aagggacgga
     1621 tggcagcatg tggacaaata tatgcacact ggttacattg aatgcagttt tcggggatcc
     1681 actcgggcac ggttacggct atgccgcgtt tttcgtattt ggttgtaccg gccgggaaag
     1741 taccatcttc tctatctaag aatacactta cgggaaggtc gtctcccttt tgggcattca
     1801 taggttctac cacttttttg atgaattcag gcagaggacg ttcgtctttc tcgacttcaa
     1861 tttgaatgtt tgcccattca gcaggtattt ctatcttgat gacgtctcca ccgcggtcaa
     1921 cggctgcata attcatcctg actatatcct ctccctgtcg gccatacgac tttttgatca
     1981 tttccttcat cttctcaaca gccagatcat aagaaatgac cttggatacc ttaaagaaag
     2041 cgctttgcat gatggtattg gtacgatgtc ctaatccaat ttcttcggca attgccgtag
     2101 cattaatgat ataaaaagag atattatttt cggccatata ttttttaata tggtcgggta
     2161 aacgcctttt agtttcttcc acatcccaaa tggagttgaa taggaaggtg cctccttttt
     2221 taagaccttt tagcatgtcg tatctattga ggtaagcagg cacatggcaa gccacaaaat
     2281 cgggattggt aaccagatag gtagaacgaa tggggtgctt tccaaatcgt agatgggagg
     2341 ctgtaaatcc acctgatttt ttagagtcgt aggcaaaata ggcttggcaa tataaatcgg
     2401 tttcttcacc aataattttg atagtgttct tgttagctcc aactgtaccg tccgagccta
     2461 acccatagaa tttagcttca taagttcctt ctggtgcaaa actttgctct gggagcaaag
     2521 gcaacgattt aaatgtaaca tcatctacga tgccaatcgt aaaatcgttt ttaggttcgg
     2581 gcatccgtag attatcgtaa acggcaagaa tttgagaagg tgtagtgtct ttcgagctca
     2641 atccatagcg cccacccaca atcaagggtg tattatccat gccatagaac atgctgcgga
     2701 tgtcttgata caatggttca ccatcggcac cgggttcttt tgtgcggtcg agaactgcta
     2761 tacgttttac ggatgaggga agaaccttga agaaatattt tgagctgaag ggacgataaa
     2821 gatgaacgca aatgaggccg atcttttcgc cctgtttcaa tttataatcg atgacttcct
     2881 taatagtttc agtaaccgaa cccatggcga tgataatatt ttcggcatcg agggcgccat
     2941 aataatcgaa gggatgatgt tcgcggccag taattctggt catttcattc atataatgtt
     3001 ctaccagatc gggcacggca tcataaaaac gattggaggc ttcacgagcc tgaaagaaaa
     3061 tatcgggatt ttgtgctgta ccgcgggtaa cgggatgttc aggattaaga gcccggctac
     3121 ggaaggcagc taaggcttgt ttgtcgagaa gacttgcaac ttcggcttca ccgaatactt
     3181 ctattttttg aatttcgtga ctggtacgaa aaccatcgaa gaaatgaagg aaaggaatac
     3241 gtgccttgac agtcgttaaa tgtgcaacag gagcaatatc catgatttcc tgaacgcttc
     3301 cactggccaa tagagcaaaa cccgtttggc gtgtggccat cacatcactg tgatctccaa
     3361 atatagagag tgcatgagtg gcaatggtgc gtgcgcttac gtggaatacc gctggaagta
     3421 attcacctgc cattttgtac atgttcggaa tcataagtaa aagtccctgt gaagccgtga
     3481 atgttgtagt taaggctcca ctttgcaatg agccatgcac agctccggcc gctccagctt
     3541 ccgactgcat ttcaacgact tttaccggtt ctccgaaaat gttcttgcgc ccaaaggctg
     3601 cccattcatc cacatattcg gccatggtcg aagaaggagt gatgggataa atggctgcta
     3661 tctcactaaa catgtaagct acatgcgctg ccgccatgtt cccatcgcat gtaataaaat
     3721 ttttcttttt attcattcgt tttgaattat taaagtattt attttcaata aaataacttt
     3781 tgctaagaag gcagggcaaa ggtacgaaac tttttttagt gaaattttaa taactcaaca
     3841 ctcaatcagc tgatttgata attaaagtgt ttctaaatag atcccgttct acccttgaag
     3901 catttcataa tgaagtaaac aaaataatta tcagcttgtt ataaaattat gaatcatact
     3961 gtttgaaggt aaagtacact gttgccatcg gagcaagctt ctgctattgt aatttgttag
     4021 agataataac gaccttgagg taaaatggtg aagatggatt cgctacattg aatcgttttt
     4081 ctatgccgat atcaatatgg aagaatgaaa acaaatgact caattcattc cgagctatca
     4141 gggtgattag tagcttgccc tgagttaggc gggtttaagg tatggtcgga caaatgcagg
     4201 aatttatgct ttgaatgaaa tttacaaagc gaaaaaaatc atgaagttca ttctggccgg
     4261 agccgattgc ctgcgtgaga tttcgactct ttagaagaat ggaatttttt ataatgcgat
     4321 tatgatttgt gaaatggaac aatggatgta agagatgaac tatcgtccga ttccttaatt
     4381 taaaagaatc gacagggaag aatactaaaa tccaatggta tacagccggg tacaatgtct
     4441 ggattttctg atgaagccaa attctattgc acagggacag atttcttaag gtgtaagata
     4501 ggattcgaat aggtcgcaac atctattttt gcatcatctt tcggatagtt ctaatatttc
     4561 tttcttattg cagataggat aaagacattg ccgaagggcc agatcgaccg gccagcattc
     4621 tttcttctta tgatggctta tactatcttt ctcctataaa tcctacccaa gttataccgc
     4681 ctttggggaa gcgagttt
//
LOCUS       DAOORU010000170         4656 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_21980_length_4656_cov_3.961747, whole genome shotgun sequence.
ACCESSION   DAOORU010000170 DAOORU010000000
VERSION     DAOORU010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21980_length_4656_cov_3.961747"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PLF20_09170"
     CDS             <1..779
                     /locus_tag="PLF20_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81207.1"
                     /translation="IDRIDDDANLFMMGGQLKSSEAGPCNATLDSVRIINDTIVYYIS
                     YDGLNCNGKVHRTGNLEIRIKQGIFWVQPGASIKVKIINYTVTRPKMGKTTILNGEKI
                     LTNVTGGHVGLLGITYDTIIHEMTGSFQMTFDDNTTRTWNIARRKTFTGTATNMDISI
                     EGLATSGEYTSLVCWGQNRKGDDFYTQIIQPVILKQSCDFDPILGQKKHSIPSKSATS
                     TVTFGYNDNNQPIGEGECPTKYRLDWEHNGNSGTVYLFLH"
     gene            complement(997..2775)
                     /locus_tag="PLF20_09175"
     CDS             complement(997..2775)
                     /locus_tag="PLF20_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81208.1"
                     /translation="MKKISLSLIFILTVFFIHAQSEMLHQICLKQNKANDFFDLLSKT
                     TGQSEGNAILFIVPPSNCPRCDALIHTFNLSLAQNVIEVNRYAIFITDEIVAAKEYLT
                     ENKVWDIDNKNSYFFMSQEKFHEYFNSSTAEIIQVPFIAGVNFSKKTITYSASILGSS
                     LDEKMVVDIKDSFTKNIELICDKNNKYDSDYEVESKIISGIEPPLFKSAKSPQLYHRH
                     LIDTADDRFTLSELYNINIHDSIIFGNDHMTNSVVEFRLSDNKALKFKKIGPSDFERT
                     YFFANNIPQQLKEYLLNLSITRVMYFSPFLHNSYLYIPASLPYLSFIDSTLTYFNMAC
                     LVIKTFPEGKFISIEVIGRRDSLTELSGRSDVGVSHLRCRISNHNTYILPAERNAYYS
                     KGGYIPDSIGCDIDPKCDEFYNFTPLFMEMSLDGKPLRFIGNLDPLFRRIKCGYSRCE
                     QIYKTYKDGYLITDGYSGRIHIYDNYDNKIQEITLFTPDIPYKNIPDFNSSSKKYSKW
                     LAQVLPYRISDMYSDGKDIYCTILSSHRVYLCQYDLNKDKFIKISCIEQPEGNYSEAI
                     LYEVLPNKFYCLTYNKKSMNFEIFSF"
     gene            complement(2849..3280)
                     /locus_tag="PLF20_09180"
     CDS             complement(2849..3280)
                     /locus_tag="PLF20_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81209.1"
                     /translation="MKRVILIWLILPIIYMSAETVKTPPSDNKRENASQAVNIPFIIL
                     GKGGLVYEPGHNPQICPQFSFHKCASGTITIRELWNLIWGDNFQSTIDQYLPLNASVT
                     VYNDDQTTTSCSITIYGFNGSISIDNDTLVIPSNVINFSIY"
     gene            complement(3277..3633)
                     /locus_tag="PLF20_09185"
     CDS             complement(3277..3633)
                     /locus_tag="PLF20_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81210.1"
                     /translation="MNKLTRIILMVTIICIIPTMLSSKPITIHLTIYGKGGSTTNGTT
                     LTVCPITDPSKCAELTVTLELKDGESLNEQDLIGTPSNIYLNDGSIFTGSILNIDNFA
                     SVDEYNYIGNSITVEL"
     gene            4207..4593
                     /locus_tag="PLF20_09190"
     CDS             4207..4593
                     /locus_tag="PLF20_09190"
                     /inference="COORDINATES: protein motif:HMM:NF015581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HOX81211.1"
                     /translation="MNYYFTKTLNCTFEEAESRVREGLSKVGMGIVMEIDFREKFKEK
                     LNVDFRPYKVLGACSPGHAYRSILKEDKLGVLLPCNVLIQQIGDKLTEVSVMDPEGAM
                     MAVNNPEVKCITDEVRIKLQHFIEEL"
BASE COUNT         1597 a          743 c          788 g         1528 t
ORIGIN      
        1 aaatcgaccg catagacgac gatgctaatc tctttatgat ggggggacag ctaaagtcga
       61 gcgaagcagg accttgcaat gctaccctcg attctgtcag aattattaac gacaccatcg
      121 tttattacat atcctacgat ggattgaact gtaatgggaa ggttcatcgc acgggaaatt
      181 tagaaatcag aataaagcaa ggtatttttt gggtacagcc cggagccagt atcaaggtaa
      241 agatcattaa ttacaccgta accaggccaa aaatgggtaa aacaaccatt ttgaacggag
      301 aaaaaattct aactaatgta accggtggtc atgttgggct tttaggaatt acttatgata
      361 cgataattca tgaaatgaca ggttctttcc agatgacttt cgatgacaat actacccgca
      421 catggaatat tgcccgcaga aaaaccttta cgggaacggc caccaacatg gatatatcca
      481 ttgaaggctt ggcaacatca ggagaataca catcacttgt atgctgggga caaaatcgga
      541 agggagatga tttttatacc caaattatcc aacctgtgat attaaaacaa agctgtgatt
      601 tcgaccctat tttgggacag aagaaacatt ctattccctc caaatcggct acttcaacgg
      661 ttacttttgg atataacgat aacaatcaac ctattggtga aggtgaatgc ccaaccaaat
      721 atcgtctgga ttgggaacat aatggaaatt ccggcaccgt ttatcttttt ctgcattgag
      781 tagtggttaa taaacaagca cggaaataat ggggtacttc gtatcccatt atttttttac
      841 ataaagcact ataatatatt atccttaaat gttttatcga attttatatt atttatccaa
      901 ataaaatagc ttttcaatat tttagcatct aaggtatgag aaagattata cccttattaa
      961 tagtaccttt attcttgaat ttctttattt tttaaattaa aaactaaaaa tctcgaaatt
     1021 cattgatttt ttattgtagg tcaaacaata aaatttatta ggtaaaactt cgtacagaat
     1081 ggcttctgaa taattgccct cgggctgttc gatacaggaa atctttatga atttatcttt
     1141 attcaaatca tactggcaca aataaactcg atgagagctc aaaatagtac aataaatgtc
     1201 tttcccatct gagtacatat cagaaatccg ataaggcaat acttgagcca gccatttaga
     1261 atattttttg gatgaagagt tgaaatcggg gatgttttta tatggaatat caggagtaaa
     1321 taaggtgatt tcctgaattt tattatcata attatcataa atatggattc ttcccgaata
     1381 gccatcggtt atcaaatagc catccttata tgtcttatat atttgttcgc aacggctata
     1441 cccgcatttt attcttctga acaagggatc gagatttccg ataaaacgta agggtttgcc
     1501 atcgagactc atttccatga aaagaggagt aaaattgtaa aattcatcac attttggatc
     1561 gatgtcgcaa ccaattgaat caggaatgta tccgcccttt gaataatatg catttctctc
     1621 ggcaggaagt atataagtat tatgattact aatacgacat cgcaaatgtg aaaccccaac
     1681 atcacttcta ccactaagct ccgtcaaaga atctctccgg cctataactt ctattgaaat
     1741 gaattttcct tctggaaaag ttttaataac caaacatgcc atattaaaat aggttagtgt
     1801 agaatcgata aaggaaagat aaggcaacga agcgggtatg tataaataac tattgtgtaa
     1861 aaaaggagaa aaatacatca ccctcgtaat ggataaattt agcaaatatt ctttcaattg
     1921 ctgtggaata ttatttgcaa aaaaataagt tctttcgaaa tcggagggtc caattttttt
     1981 gaattttaat gccttattgt ctgataatcg gaattctacc acggaattag tcatgtggtc
     2041 attaccaaaa ataatggaat catgaatgtt gatgttgtat aattccgata gagtaaatct
     2101 atcatcggca gtatcgatta gatggcgatg gtatagctgg ggtgatttgg cacttttaaa
     2161 caaaggaggt tctattccag aaataatttt tgattccact tcatagtcag aatcatattt
     2221 attattttta tcgcaaatca attcaatgtt tttggtaaat gaatccttaa tatcaactac
     2281 catcttctca tctaatgatg aacccaaaat cgatgccgaa taagttattg ttttcttcga
     2341 aaaatttact ccggcaataa atggaacttg aattatttca gcagtagaag aattaaaata
     2401 ctcatggaat ttctcctgtg acatgaagaa ataagaattt ttgttatcta tatcccagac
     2461 cttgttctcc gtgagatatt ccttggcagc aacaatttca tcagtaataa aaatagcata
     2521 tctgttcacc tcgatgacat tttgtgccag gctcagatta aaggtgtgga taagcgcatc
     2581 acaccttgga caattagaag gaggaacaat aaatagaatg gcattgccct cgctttgacc
     2641 cgttgttttg ctcaataaat caaaaaaatc atttgcctta ttctgtttga gacagatttg
     2701 atgaagcatt tcactttgag catgtataaa gaaaactgtt aaaataaaaa taagagaaag
     2761 agaaattttt ttcataacaa attttataat ttattgtaaa tttaattctt tattcaataa
     2821 agggggcatt tctgccccca taaaagtttc agtaaattga aaaattaata acattagagg
     2881 ggataaccag tgtatcatta tcaattgaaa tggaaccatt gaatccgtaa attgttattg
     2941 aacaactggt tgttgtctga tcgtcgttgt aaacagtaac cgatgcattt aatggaagat
     3001 attgatcaat agttgattga aaattatcac cccaaataag attccataac tctcgtatgg
     3061 ttatagtacc agatgcacat ttatgaaaag aaaattgagg acaaatttga ggattatgtc
     3121 ctggttcata gactaagccc cccttgccaa gaataataaa aggaatattt acagcttgcg
     3181 aagcattttc ccttttatta tcactaggtg gcgttttaac tgtttcagca ctcatataaa
     3241 ttataggtaa tatgagccat attaatataa cccttttcat aattcgacag ttatactatt
     3301 acctatataa ttgtattcat caacagaggc aaagttgtct atgtttagaa ttgaacctgt
     3361 aaatatggaa ccatcgttaa gataaatatt acttggggtc ccaattaaat cctgttcgtt
     3421 taagctttca ccatctttta attccaaagt tacagttaat tcagcacatt tacttggatc
     3481 ggttatagga catacggtga gcgttgttcc attcgtagta ctacctcctt tcccataaat
     3541 tgtcaaatga atagtaatgg gtttagacga aagcattgtt ggaattatac aaattattgt
     3601 caccattaaa ataatacgag ttaatttgtt cataatattg tttttaagtt ttgttaatta
     3661 ttaattttgt aaattttaaa gataaaagag gaaatatatt aatcctctat aagttaatat
     3721 gcatctaatg tttttagtcc tcataaaatt tatgaggact aaaatcgaat gaatttattt
     3781 ttcccaagaa taaatatatt aataaataaa tagataatat atatatatat atatatatat
     3841 atataaataa atatataata tatatatata tatatatata tatatatatt atccaaaaca
     3901 aattctaaac tttcttttat ttcgtttttc tttttctcgc accttttaaa attgaaggga
     3961 tacatcttta ttagattttt cgatacaaag ataaggcatt tttttcaaaa gtcaacacct
     4021 tttcaaaaat ttaacaaaat ttaatatgag aaactttaat attgggttgt taagattctt
     4081 ttcggatgaa acgcaatgga agcagaaaat catttcccag gctttttggc aaataagatt
     4141 tctgatattg aacctgatat tgaagatgag caattttttt ttatttttgc aataaatgat
     4201 gtaaccatga actattattt taccaaaact ttgaattgta cttttgaaga agccgaatcg
     4261 cgtgttcgtg aagggctatc gaaagtaggc atgggtatag tgatggaaat agattttcgt
     4321 gaaaaattca aggaaaaact caatgtcgat ttcaggcctt ataaggtctt aggagcttgt
     4381 tcacccggtc atgcttacag atctatcctc aaggaagaca aactgggtgt tttgctgccc
     4441 tgcaatgttc ttattcaaca aattggggat aagctgaccg aagtttcggt catggaccct
     4501 gaaggagcca tgatggccgt gaataacccc gaagtaaaat gcattaccga cgaagtgagg
     4561 ataaagctgc aacatttcat cgaagagctt tagatagaaa atagtttgtc tcgagtcctc
     4621 gaaatgtcga gcgaaaagga gattttttat aacttc
//
LOCUS       DAOORU010000171         4602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_22310_length_4602_cov_4.855729, whole genome shotgun sequence.
ACCESSION   DAOORU010000171 DAOORU010000000
VERSION     DAOORU010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22310_length_4602_cov_4.855729"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1846
                     /locus_tag="PLF20_09195"
     CDS             233..1846
                     /locus_tag="PLF20_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008780255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOX81212.1"
                     /translation="MQKLVLLGDEAIGQAAIDAGLSAIYAYPGTPSTEINEYVMASKE
                     AKEGRIIASWTVNEKTAVESALGMSYSGKRSMACMKHVGLNVAADPFINSAITGVNGG
                     MIVVSADDPSMHSSQNEQDSRFYGNFAMLPILEPSNQQEAYDMIHYGFDLSEKFHVPV
                     LFRITTRLAHSRSGIERKAVRTQNPISLPENLKQFILLPAIARKRYQVLLSIQDALEE
                     ESENSGNNVYIDGSDKSLGIITTGLAYNYLMENFPDRTVPHPVLKITQYPLPRKMLQK
                     IVSECKEILVIEEGMPVVEETLKGILDHGIKIKGRLDGSLPRSGELNPNLVAVALGKP
                     MIKGDDVPSLIVGRPPSLCEGCPHTDSYFALNEAMAEYGKGRVFSDIGCYTLAALPPL
                     EAINSCVDMGASIPMARGAADAGLLPAVAVIGDSTFTHSGMTGLLDAVIANSPITVMI
                     LDNSTTGMTGGQPSAATGRLKQICLGLGVEENHIRILKPLRKQHTENVRAIKEELSYQ
                     GVSVIIPTRECIQTLNRRMREKFKNKKTE"
     gene            1887..2462
                     /locus_tag="PLF20_09200"
     CDS             1887..2462
                     /locus_tag="PLF20_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009131087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOX81213.1"
                     /translation="MKNDIIIAGVGGQGILSIAAIIATAAVNNNLYIKQSEVHGMSQR
                     GGEVQSHLRISSSPIASDLIPTGAADMIISLEPMEALRYIPWLKPDGWIITNTEPFLN
                     IPNYPPIEDVLAEIKKRPHHIMMNADEVARELGSEKAANMVILGAATRCLDIPYEEIE
                     KAIRNFFGRKGEEIVKMNLSALAKGREMALL"
     gene            complement(2570..4522)
                     /locus_tag="PLF20_09205"
     CDS             complement(2570..4522)
                     /locus_tag="PLF20_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HOX81214.1"
                     /translation="MKKILFFAFLISTLSIYADDAVRQALRRSGNSTKYPNDNVLVVF
                     DSTKVEVAPTGLAKINIHQLFKVLNTKGALEKRVIKMGYDPLTSYVKILSVSIYKKNG
                     DIINLDTSRVKDYPAPARSIYWGAREKMLEIGYLEPGDGIEVKLFRKGFTYALLNDEP
                     DDERYIPPMRGHFYDIVPFWSSNPIVEKTYIASLPLEKNLQYEFYNGEIRSSVIREGE
                     KMIYTFTKTNIQPMKAEPNMVAWSDVAPKLLLSTSPDWKAKSLWFYHVNEDYGSFETT
                     PEISAKTREILRSARNELDSISLLTHWAADEIRYSGLSMGEGEGYTLHKGAMTFADRC
                     GVCKDKAGILITLLRAAGFESYAAMTMAGERIERIPADQFNHSITIVKRRNGEYMLLD
                     PTWVPFIRELWSSLEQQQGYLMGVPEGADLKYTPISDPENHPVKIKGSSKILNDGTLE
                     GWLEITGEGQSEAAIRSVFTGVARCEWEKNLENELLRMHPRAKIIEKSYSNPDLYLEQ
                     PIIIHMMYSIPNYALIGDSNILFQPLLASGIFQRAQPQLGMNLDIDERIYPFRDRCSR
                     KVEVEETIEIPEGFALLKPDYQINIIGDASSIKASMQTEGKRISISQVALYNKRIYQA
                     EEWSNFKEVVKAQRKFKDSPLILKKL"
BASE COUNT         1317 a          963 c          997 g         1325 t
ORIGIN      
        1 tgttgataac taaaaaacac aaatcaattg cacctctttt cgatgaaaaa ttttggaaaa
       61 atttttaggg tgacgcattg tcgaagtcag gagacaagat tcaatgagta gggtctgaca
      121 ttcatcttac attgattggg taggtagttt ctcttgattt tctttccagc aaattaaaaa
      181 tccttagatt tgtaggccga gggaaatgat aatttttcaa tcattttaag atatgcaaaa
      241 acttgtatta ttaggagatg aagccatagg acaggcagcc atcgacgctg gattatcggc
      301 aatttatgct tatccaggaa cgccttccac tgagatcaac gaatatgtga tggcatcgaa
      361 ggaagccaaa gaaggaagaa ttatagcaag ctggacagtc aacgaaaaaa ctgcagtgga
      421 atcggctttg ggcatgtcgt attcagggaa acgtagtatg gcttgtatga agcatgtagg
      481 tttgaacgta gccgcagacc cctttatcaa ttcagcaatt acgggagtga atggaggcat
      541 gattgtggtt tcggctgacg acccctccat gcatagttca caaaatgaac aggattctcg
      601 tttttacgga aattttgcta tgcttccgat tttagaacct tccaaccaac aggaagccta
      661 tgacatgata cattatggtt tcgatttgtc ggagaagttt catgtacccg tactgtttcg
      721 catcacaact cgactggcgc atagccgttc gggaattgaa cgaaaagcgg taagaactca
      781 aaatcccatt agcttgcccg aaaatctgaa acaatttata ctcttgcctg ccatcgcccg
      841 caaacgttat caagttctat taagcattca ggatgccctg gaagaagaat cggaaaattc
      901 tggaaataat gtttatatag atggctcgga taaatcattg ggaattatta ctacgggttt
      961 ggcatacaat tatctgatgg agaatttccc cgatagaaca gtcccacatc ccgtcttgaa
     1021 aatcactcaa tatcctttac cacgaaaaat gttgcaaaaa atagtttcgg aatgcaagga
     1081 aattttagta atcgaagaag gcatgcccgt tgtggaagag actctgaaag ggattttaga
     1141 tcatggtata aagataaagg gtcgtttgga cggaagttta ccccgcagtg gagaattgaa
     1201 cccaaactta gtggctgtgg cattgggcaa acccatgatc aagggggatg atgttccctc
     1261 gcttattgtt ggtcgtcctc cttcgctttg tgaaggatgc ccacataccg attcttactt
     1321 cgccctaaat gaagccatgg ctgaatatgg caaagggaga gtattttccg atattggctg
     1381 ttatacgctt gcggcattgc ccccgcttga agccattaat tcatgcgtag atatgggagc
     1441 ttccatccca atggctagag gtgcagccga tgctggtctt ttacccgcag tagcggtcat
     1501 tggagattct acttttacac attccggaat gacgggctta ctggatgcag taatcgccaa
     1561 ttcacccatt acggtgatga ttctcgacaa ttctactacg gggatgacgg gaggacaacc
     1621 ctcggctgca accggccgtc ttaagcaaat ctgtttggga cttggagtgg aagaaaacca
     1681 tatccgcatc ctgaaaccct tacgcaaaca acataccgag aatgttcgag ccattaagga
     1741 agaattgtcc taccaaggcg tttcggtcat cattcctaca cgtgaatgca tacaaacact
     1801 caatcgtcgc atgcgcgaaa aattcaaaaa caagaaaacc gaatagacga ttttaaacat
     1861 tactcaaaac ttaaacagaa ttcaatatga aaaacgatat catcatagct ggtgtgggcg
     1921 gacaaggtat tctttccatc gcagccatta ttgccactgc cgctgttaat aacaacctct
     1981 acatcaaaca atcggaagtt catggaatga gccagcgcgg gggagaagta cagtcacatc
     2041 tgcgtatttc gtcttctccc attgcctctg acttgatccc tactggagct gccgacatga
     2101 tcatttcact cgaaccgatg gaagccctcc gttatattcc ctggttaaaa ccagatggct
     2161 ggatcatcac caatactgag cctttcctga atattcccaa ttatcctccc atcgaggatg
     2221 ttctggctga aatcaagaaa aggcctcatc acatcatgat gaatgccgac gaggtagctc
     2281 gtgaactggg ctcagaaaaa gcagccaata tggtgattct gggagctgca acacgatgct
     2341 tggatattcc ttacgaagaa atcgaaaaag ctattcggaa cttcttcgga agaaaaggag
     2401 aagaaatcgt aaaaatgaac ttatcggctc ttgcaaaagg cagagaaatg gcattattat
     2461 aaatagtgga tataaggttg gtggttgaaa agagactgga ggacgaaagg gtaatctgtt
     2521 ttccaacctc caatcacctt ctcacgggcg ttcttttatc gagtgatttt tacagttttt
     2581 tcaaaatcag gggtgaatct ttgaattttc tttgggcctt tacaacttcc ttaaaattgc
     2641 tccattcttc ggcttggtag attcttttgt tgtaaagagc aacttgagag atagaaatgc
     2701 gtttgccttc ggtttgcatg gaagccttta tactggatgc atcaccaatg atatttatct
     2761 gataatcggg tttaaggagt gcaaatcctt ccgggatctc aatggtttct tccacttcta
     2821 ccttgcgcga acagcggtcg cggaaagggt agatacgttc atcaatatcc agattcatac
     2881 caagttgagg ttgagctcgt tggaaaattc cggaagccaa taatggctgg aagaggatgt
     2941 tggaatctcc aatgagggcg tagttgggaa tgctatacat catgtgaata ataatgggtt
     3001 gttccagata aagatcggga ttggaatagg atttttcgat gattttagct ctgggatgca
     3061 ttcgcagcag ttcattttcg aggttctttt cccattcgca tcgtgcaacc cctgtaaaaa
     3121 cacttctgat ggctgcttcc gattgtcctt cgcctgtaat ctctaaccag ccttccaaag
     3181 tcccatcatt gagaatttta gagcttcctt tgattttcac aggatgattt tcgggatcag
     3241 aaatgggagt gtatttcaaa tcggcgcctt cgggaacacc catgagataa ccctgttgtt
     3301 gctcaaggct cgaccatagt tcacgtataa aaggaaccca tgtaggatca agcagcatat
     3361 attcgccgtt gcggcgcttt actatggtta tggaatgatt gaactgatcg gcaggaattc
     3421 tttcgatgcg ttcacctgcc atcgtcatgg ccgcataaga ttcgaaacca gctgcccgca
     3481 ataatgttat gagtatacct gccttgtctt tacaaactcc gcagcgatcg gcaaaagtca
     3541 ttgccccttt atgcagggta tacccttcgc cttctcccat cgaaaggcct gaatatctta
     3601 tttcatcagc cgcccaatgg gtgagcaaac tgatagaatc gagttcatta cgtgcagagc
     3661 gaagtatttc cctggttttg gccgagatct ctggagtggt ttcaaaactg ccataatctt
     3721 catttacatg ataaaaccat aaagatttgg ctttccagtc gggacttgtc gaaagcaaga
     3781 gtttgggtgc cacatccgac caagctacca tattgggttc ggctttcata ggttggatat
     3841 tggtcttggt gaaagtataa atcatttttt ctccctcacg aataaccgat gaacggattt
     3901 caccattgta aaattcatac tgaagatttt tttcaagtgg caggcttgca atataggttt
     3961 tttcgactat gggatttgac gaccaaaatg gaacgatgtc atagaaatgt cctctcatag
     4021 gtggaatata cctttcatca tcaggctcat cgtttagcaa ggcataagtg aatcccttgc
     4081 gaaaaagctt cacttctatc ccatctcccg gctcgagata tccgatttct aacatttttt
     4141 cacgagctcc ccaataaatc gaccttgctg gggcaggata atcctttacc cgggaagtat
     4201 ctaaattgat gatgtctcca ttttttttat agatggaaac ggataaaatc tttacataag
     4261 acgtaagagg gtcgtatccc attttgataa ctcttttttc gagagctcct ttggtattta
     4321 gcaccttgaa caattgatgg atgtttatct tggctaatcc tgtcggggca acctctacct
     4381 ttgtactatc gaaaaccacg agtacattat cgttgggata ttttgtcgaa ttcccacttc
     4441 tgcgcaatgc ttgacgtaca gcatcgtctg cataaatgct caatgtgctg attaagaatg
     4501 caaaaaatag aatctttttc acttttattc gaattttatt tcatgttcaa atttacatga
     4561 aaactttcga ttgacacaca gacgaataaa aatccagtcc gt
//
LOCUS       DAOORU010000172         4572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_22500_length_4572_cov_6.295772, whole genome shotgun sequence.
ACCESSION   DAOORU010000172 DAOORU010000000
VERSION     DAOORU010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22500_length_4572_cov_6.295772"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1189)
                     /gene="lpxD"
                     /locus_tag="PLF20_09210"
     CDS             complement(143..1189)
                     /gene="lpxD"
                     /locus_tag="PLF20_09210"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOX81215.1"
                     /translation="MEFTAREIAAMLNADIEGDPETRINNLAKIEESNPGTLTFLANP
                     KYIPYIYTTKASLVLVGKNFEPEHPIQSTLLRVDNPYVAFAFLLEKFNQIFLNRQGIS
                     PQAFIHPSAHIGKNVYIGEFSYIGEDTEIGDEAKIYPLTYVGDHCEIGEKTTLFSGVK
                     VYSMNIIGRECTLHSGVVIGADGFGFAPQGNDYQKVPQIGNVIIKDHVEIGANTTIDR
                     ATLGSTIIHKGVKLDNLIQVAHNVEIGENTVIASQTGISGSTKIGANCMIGGQVGIIG
                     HISIADEVKIAAQSGIGGSITQKGAIVQGSPAFEYKKYQKSYVHFRNLHQLYEKINQL
                     EERLKELEQLNKDE"
     gene            1468..1797
                     /locus_tag="PLF20_09215"
     CDS             1468..1797
                     /locus_tag="PLF20_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOX81216.1"
                     /translation="MTLNEAQQKVDQWIRTIGVNYFDELTNLALLMEEVGEVARIMAR
                     KYGQQSAKPEDVSTTLADEFADVLFVLICLANQTGVNLTEAFRSNLEKKTTRDALRHQ
                     NNPKLKK"
     gene            1871..4363
                     /locus_tag="PLF20_09220"
     CDS             1871..4363
                     /locus_tag="PLF20_09220"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOX81217.1"
                     /translation="MLKSLCLSMNFLRSYILRSFIKLIPRFLAACFLLNIPMVYAQVL
                     YSISGKVVDAHNGLPLAFVNIQVNDKMVGTVSDIDGNFNFSSSQPIEKLRFSYIGYEP
                     LVLEQSSLKQKIIVKLRKTEIELKEVTVYPGQNPALRIIRNALNNRQRNNIENLPGFS
                     YNAYEKTVFSIKVDSLLLSDTNSLDTNLRQLKEFVNRQHLFIIENYIERQYKSPRKNS
                     EKVVATKISGLKDPIFVFLLSRLQSSTFYEDVFSIGGQNYINPLAENSIDKYFYQLED
                     TIISGQDTTYIIFYRPWKGKNFDGLKGVLHINSNGWAISSVTAEPYQKDENFEIKVQQ
                     LYAQDSNGIWFPRQLNMDLIMNIVQVNAGNKQTPFRIIASGKTYIENFRVIESSVKNI
                     SSHIEIDVIPEAAYRDEDYWQRVRAVPIDEREKETYRVIDSLGAVQNLDKFTYTLDAF
                     MKGKIAIKKVNLDLEKIMHYNAHEGFYAGLGLETNDLFSRRWQTGAYWGYGFKDKTAK
                     WGTFLRLNLSSLHDSYFRYDYRFDKQESGATEFTGRNLSEFDPYTFRNFQINRMDGLN
                     AHQLIFSTRIFEYTNVWLAMSIAQMNPAYDYRFQYQEFAKNETFGFSELSASFRWAYE
                     EKFIRTTHGKVSLGTKFPIVWFQYSKGLDFLPGGDFDYFRSLFDITYSFYTPFVGKTS
                     FRLNGGMSSSNLPAGKLFNARANHADFTLFSPGSFVTMRMNEFYSDRFIALFVQHNFG
                     HLLFKSGKFQPDLEIFSSALFGSLKHPEYHEGISMKVAEEGYFESGLLIHRLIDFGFY
                     NLGLGAAWRYGTYSLSKLSDNLALMLSLRFAM"
BASE COUNT         1383 a          915 c          879 g         1395 t
ORIGIN      
        1 aattcccccg tatgcaggcc ggtgcccgag atagttactg aagaggctat cgttttttgt
       61 ggatcatttg tcgacggcat ttccttatga gattaaataa tttgcaaagg tagaaaatgt
      121 gcaaaaactg aagaagtcga agctattcat ctttattgag ctgctcaagc tctttcagcc
      181 tttcttctaa ctggtttatc ttttcataga gttgatggag gtttctgaaa tgcacataag
      241 atttttggta ttttttatat tcaaaagcag gacttccttg cacaatggct cctttctggg
      301 tgatagaacc tcctatcccc gattgggctg caatttttac ttcatcggca atactgatgt
      361 ggccaatgat cccaacttga cctcctatca tacaattagc tccgattttc gtcgaacccg
      421 aaattccagt ttgagaggct attacagtgt tttcgccaat ttcgacatta tgagccactt
      481 gtattaaatt gtcgagcttt acacccttat gaataatcgt acttcctaaa gttgcccggt
      541 caatagtagt attggcacca atttccacgt ggtctttaat gattacgttg ccgatttgag
      601 gtactttctg ataatcgttg ccctgaggag caaagccaaa accatcggcc ccaatcacca
      661 cgcccgaatg caatgtgcac tccctaccaa taatattcat gctgtaaacc ttgactcccg
      721 aaaacaatgt agttttttcg cctatttcgc aatgatcacc tacgtaagtc aaaggataaa
      781 tctttgcctc atcgcctatt tcggtatctt cgccaatata ggagaattca ccaatataaa
      841 cgttcttgcc aatatgagcg ctgggatgta taaatgcctg aggcgatatt ccctgcctgt
      901 tgagaaaaat ctgattgaat ttttccagta aaaaggcaaa agctacataa ggattgtcaa
      961 ctcgcagaag agtggattga atggggtgtt caggctcgaa atttttgcct acaagtacca
     1021 atgaagcttt ggtggtatat atataaggta tatattttgg gttagctaaa aaagttaatg
     1081 tacccgggtt cgactcttcg atcttcgcca gattattgat ccgcgtttct ggatctcctt
     1141 cgatatcggc attaagcatg gcagctattt ctcgggcagt aaattccatt tttctatcgt
     1201 attatgagag tacaaaagta gatgattgtc gtaaataatg aaatttcaca tttctactaa
     1261 ttttataccg aaatctcaca ataagacaat caaggtgaaa aatttttatt ctttccttat
     1321 gaaattttaa caaagaacta tatttcaaca caagaacacc tctgggaaga tcttgttgtt
     1381 agcattaaat aacaaattat gccacagttt tattataaaa tcatttattt tgaagtcctg
     1441 aatcataaag gtaaaattta aagaaatatg acgcttaacg aagctcaaca aaaggtagat
     1501 caatggataa gaaccattgg agtaaattat ttcgatgaat tgaccaatct ggcgttattg
     1561 atggaagaag tgggagaagt agctcgtatt atggcgagaa aatatggcca gcagtccgcc
     1621 aagcctgaag atgtatccac cactctggcc gatgagtttg ccgatgtttt attcgtgttg
     1681 atatgtctgg ccaaccaaac cggagtaaac cttacagaag catttcgttc gaatctcgaa
     1741 aagaaaacta cacgtgacgc tttacgccat caaaataatc ccaaactgaa gaaataataa
     1801 cctattcatt tattctaact ctggatgaaa atattcgttt ttaatttttc ataaaaatcg
     1861 aaatcgagga atgttgaaaa gcctttgttt gtctatgaat tttctgcgtt cttatatcct
     1921 tagaagcttt attaaactga taccccgctt tttagcggcc tgtttcctct tgaacatacc
     1981 aatggtatat gcgcaagttc tctattctat ttcgggaaaa gtagtggatg cccacaatgg
     2041 gttgcccctg gcctttgtaa atattcaggt aaacgacaaa atggtgggca cagtgagcga
     2101 cattgatggg aattttaact tttcctcatc acaacctatc gagaaattac gattttcata
     2161 tatcggctat gaaccactcg tattagaaca atcgtcatta aagcaaaaaa ttatcgttaa
     2221 gctcagaaaa acagaaatag aattaaaaga ggttaccgtt tatccaggac aaaatccagc
     2281 actacggatc atccggaatg ccttgaacaa tcgccaacgc aacaatatcg aaaatttacc
     2341 gggtttttcg tataatgctt atgagaagac agtattcagc atcaaagttg attcactttt
     2401 gctttcagat acaaattcgc tggatactaa cctgaggcaa ctaaaggaat tcgtaaaccg
     2461 acagcatcta tttattatcg aaaactatat cgaacgccag tataaatcac cacgtaagaa
     2521 ttccgaaaaa gtagttgcca ctaaaatttc gggtttgaaa gaccccatat tcgtatttct
     2581 acttagccgg ttgcaatcga gtacatttta tgaagacgtt tttagcattg gtggccaaaa
     2641 ctatattaac cctttggctg agaacagcat agataaatat ttttatcaac tcgaagacac
     2701 aattatcagt ggtcaggata ctacttacat tattttttac cgcccatgga agggaaagaa
     2761 ttttgatgga ttgaaagggg ttttgcatat caatagcaac ggttgggcaa tttcgtcggt
     2821 tactgccgaa ccctaccaaa aagacgaaaa cttcgagata aaggttcaac aactatatgc
     2881 acaggactcg aatggaatat ggtttccgcg ccagctaaac atggatttga tcatgaatat
     2941 tgttcaggtg aatgctggta ataaacagac gccgtttcga atcatcgcaa gcggaaagac
     3001 ttatatagag aatttcagag ttatcgaaag ctcagtgaag aacatttcct cgcatattga
     3061 aatagatgta atacccgaag ctgcgtatag agacgaggat tattggcaga gagtaagagc
     3121 agtacccatc gacgagcgag aaaaggaaac ctaccgtgtg atagatagct taggcgccgt
     3181 ccaaaatctc gacaagttta cctatacgct cgatgctttt atgaaaggaa aaattgctat
     3241 caaaaaagta aatcttgatc tggaaaaaat catgcattac aacgctcacg aaggatttta
     3301 tgccggtttg ggattagaaa ccaacgatct cttttcccgt cgctggcaaa ccggggcgta
     3361 ttggggttat ggattcaagg acaaaacagc caaatgggga acatttcttc gacttaatct
     3421 ttccagcctg catgattctt attttcgcta tgattataga ttcgataagc aagaaagcgg
     3481 agcaaccgaa tttacaggac ggaatttgtc ggaattcgat ccttatacct tccgaaattt
     3541 ccagattaac agaatggatg gcctcaatgc acatcaactc atcttttcaa cccggatatt
     3601 cgaatatact aatgtctggt tagccatgtc catagcacaa atgaaccctg cctatgatta
     3661 cagatttcaa taccaggaat ttgctaaaaa cgaaacattt ggtttttccg agctatcggc
     3721 ctcttttcgc tgggcttatg aggaaaaatt cattcgtacc acacatggta aggtttcgct
     3781 cggaactaaa tttcccatag tttggttcca atatagtaaa ggactcgact ttttgccagg
     3841 aggagatttc gattattttc gttccctttt cgatataact tattctttct ataccccttt
     3901 cgtcgggaaa acctccttcc gcctgaatgg tgggatgtct tcttcgaatc ttccggcagg
     3961 taaactcttc aatgcgcgtg ccaaccatgc cgattttacg ctgttttcgc ccggaagctt
     4021 cgtaaccatg cgcatgaatg aattctattc cgaccgtttt atagccctat tcgttcagca
     4081 taactttggt catctattat tcaaatcggg aaaatttcaa cccgacctgg agattttttc
     4141 ttcggctttg tttggaagtc ttaaacatcc agaatatcat gaaggcattt cgatgaaagt
     4201 tgccgaagaa gggtacttcg aatcaggcct gcttatccac cgtctgatcg attttggctt
     4261 ctataattta ggattgggag ccgcttggcg ttatggaacg tacagtttat ctaaactctc
     4321 cgataatttg gctctaatgc tgtcattgcg ctttgccatg taatcattcg ttgaatccat
     4381 cctaatattg tcgatattgc ttcatttcga agttcgacat gggttcttga aaataaagtt
     4441 ggtccctacc aattctgaag gttttcatgc ttgccataat taggaaattc tatttcgaaa
     4501 atactggcga cctttcataa cccaacttga gcattttacc ctgaaaaaac agggaaaaat
     4561 agggtttttg ct
//
LOCUS       DAOORU010000173         4570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_22510_length_4570_cov_5.205537, whole genome shotgun sequence.
ACCESSION   DAOORU010000173 DAOORU010000000
VERSION     DAOORU010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22510_length_4570_cov_5.205537"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PLF20_09225"
     CDS             <1..755
                     /locus_tag="PLF20_09225"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOX81218.1"
                     /translation="IHMQEDGLIYGGAPGVALTWMDAIIEGVPITPRIGCPVEANALW
                     YNGISFAIAAARRASDKVFVERWTPVIKRLNNNFPANFWDEDKGYLADYTDGNFKDWT
                     MRPNQVIATAMQYTPLKEEIRKGILSRVRHELLTPRGLRTLTPTHPDYKGIYAGNQID
                     RDLAYHQGSAFPWLLGFFVEGYLRIHGKGGLSFIKNLYNGFEPEMTEHGLCTISEIYD
                     GDPPHKPAGAISQAWSVAALLTMHDLIEHYEK"
     gene            785..2083
                     /locus_tag="PLF20_09230"
     CDS             785..2083
                     /locus_tag="PLF20_09230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOX81219.1"
                     /translation="MRVLMFGWEFPPHITGGLGTACYGLTKGLMKHGVEVIFVVPKAY
                     GDEDQSAVRLVDASDIVVDPNDSEFKGLWDRIKYMEISSTIIPYVSPEKFEDMKIREE
                     LNKHRDEQHVLSRKYEFSGKYGPRLLEEVSRYAIIGSALAAKEEFDVIHAHDWLTYPA
                     GIAAKNVSKKPLIVHIHATEFDRSGQNVNPEIYEIEKRGMEEADFVISVSNFTRNICI
                     EKYHIDPDKIITVHNAVEPADNMESPTVERNLDDKIVTFLGRITFQKGPEYFVEAARK
                     VLDRYPNVRFVMAGTGDMQQKMIRRVAKLKMGTKFHFTGFLKGNEVNKLLAMTDVFVM
                     PSVSEPFGIVPLEAMQSNVPVVISKQSGVSEVLKHAIKVDFWDVNALADAIYGLLAYP
                     KLPEMATREGKSEVDNLKWENAAFLVKQVYERAIRLSSSN"
     gene            2102..3670
                     /locus_tag="PLF20_09235"
     CDS             2102..3670
                     /locus_tag="PLF20_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HOX81220.1"
                     /translation="MRSICLYFQIHQPFRLRTYRFFDIGNDSNYYDEYQNRFIIRRVA
                     EKCYLPANKLLLTLIRRHGQAFRVCFSISGTAIEQFEKYTPEVLDSFKQLAATGQVEF
                     LAETYGHSLAGLKNKEEFKRQILMHSQRVEELFGIKPTSFRNTELIYSDEIGATIAEM
                     GFYSILAEGAKHILGWRSPNYLYYNALNPRLRVLLRNFRLSDDIGFRFSNKAWSEWPL
                     TAEKYVSWLNAFDPNQELVNIFLDYETFGEHHWKETGIFEFLEALPKTILTQSNFEFH
                     TPTEIASLHQPVAPIHVPHATSWADEERDLTAWLGNELQEEAVSNLYALVSKVYACDD
                     PSIWKDWLYLQTSDHFYYMCTKWFSDGEVHKYFNPYPSPYEAYINYMNVLSDFIIRVE
                     KNSVGKLEYEGWPSLKSVYEFNNISGPPLPFVTQAPHFVRSDKKSSKLMAQPSDLIHF
                     SDRKLKSALKNTDSEFIAKIIANSAEEVTDKILRNLTKQRGEEVKELAHEIKKVSKKA
                     LSEAVEEILLHIQK"
     gene            3738..>4570
                     /locus_tag="PLF20_09240"
     CDS             3738..>4570
                     /locus_tag="PLF20_09240"
                     /inference="COORDINATES: protein motif:HMM:NF017504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HOX81221.1"
                     /translation="MAANEIIKPDYLFEVSWEVCNKIGGIFTVLATKAGTIKKELDDN
                     YIVIGPDVWMETHTNPYFEEDHLLYRAWRMQATSEGLKVRLGRFKGKKGAVAILVDFT
                     SLFPQKDKILAHLWEKFGLDSITGGWDYIEPLLFGYAAGKVIESFYEYEVFPQEKVVA
                     HCHEWMSGGTILYLKENLPQIATVFTTHATVLGRSISGHNWPLYRNLEFYNPETIARE
                     LGVVAKYSLEKTAALNTDILATVSNISAAEARKFFGRDVDYITPNGFDVSLIPDSDFE
                     T"
BASE COUNT         1412 a          900 c          978 g         1280 t
ORIGIN      
        1 atattcacat gcaagaagat ggtctaatct atggtggtgc gcccggagta gctcttacct
       61 ggatggatgc catcattgaa ggagttccta taacacctcg tataggttgt cctgtcgaag
      121 cgaatgcatt gtggtacaat ggtataagtt ttgctattgc agcggccaga agagcttccg
      181 acaaggtttt cgtcgaaaga tggactccgg taataaagag gctcaataat aattttcctg
      241 ctaatttttg ggatgaagac aaaggctatc tggccgatta tacagatggt aactttaaag
      301 actggaccat gcgccccaat caggtaattg ctacagcaat gcaatatact cctctgaagg
      361 aggaaatccg caaaggaata ttaagcaggg taaggcatga attattaacc cctcgaggct
      421 tgcgtacttt aactccaaca caccccgatt acaaaggaat atatgctggc aatcaaattg
      481 accgggatct cgcctaccat caggggagtg ctttcccttg gctattggga ttctttgtcg
      541 agggctactt gcgcattcat ggcaaaggcg gattatcgtt cataaaaaat ctgtacaatg
      601 gattcgaacc cgaaatgacc gaacatggat tgtgtactat ttcggaaatt tacgatggtg
      661 atcctccaca caagcccgca ggagctattt ctcaagcatg gagcgtagca gcattgctta
      721 ccatgcacga cttaatcgaa cattacgaaa aataaatata aatgaattaa aatccataaa
      781 agctatgaga gtattgatgt ttggatggga gttcccccct catattacag gtggtttagg
      841 aacggcctgt tatggcctta ctaaaggatt gatgaaacac ggtgtggagg tgatctttgt
      901 tgttcccaaa gcctatggag acgaagatca atctgccgta agactggtgg atgccagcga
      961 catagtcgtc gatcccaatg actctgaatt taagggatta tgggacagaa taaaatacat
     1021 ggaaatctct tccactatca ttccttatgt gagtcctgaa aaattcgaag acatgaagat
     1081 cagggaagaa ttgaacaagc atagggatga acaacacgtc ctgagccgga aatatgaatt
     1141 ttctggtaaa tacgggccgc ggcttctaga agaagtctcc cgatatgcta tcattggttc
     1201 ggccttggca gctaaagaag aattcgatgt aattcatgcc catgactggt taacttatcc
     1261 ggctggtatt gcggcaaaga atgtcagcaa gaaaccactt atcgttcata ttcatgccac
     1321 tgagttcgat cgtagcggcc aaaatgtaaa ccccgagatt tatgaaatcg aaaaaagagg
     1381 aatggaagaa gccgattttg tgattagtgt gagcaatttc acccgcaata tctgtatcga
     1441 aaaatatcac atcgatcccg ataagatcat cacagtgcat aatgccgtag aaccggcaga
     1501 caatatggaa agtccgacgg ttgaacgtaa tctcgacgac aaaatcgtca cctttttagg
     1561 acgcataacc ttccagaaag gccctgaata tttcgtcgaa gcggcacgca aggttctgga
     1621 tcgctatccc aatgtccgtt ttgtgatggc aggaacaggc gatatgcagc aaaaaatgat
     1681 acgacgtgta gccaaactca agatgggtac aaaattccat tttactggat tcttaaaagg
     1741 aaatgaagta aacaaactct tggcgatgac cgatgtattc gtgatgccat cggtttctga
     1801 accttttggg atagttccgc tcgaagccat gcaaagcaat gtaccggtcg ttatttcgaa
     1861 acaatcgggt gtgtcggaag ttctcaagca tgctataaaa gtagattttt gggatgtaaa
     1921 tgccctggct gatgccatat atggactgct tgcctaccct aaactgcccg aaatggctac
     1981 cagagaagga aaatcagaag ttgacaatct gaaatgggaa aatgcggctt ttctcgtaaa
     2041 acaggtctat gagagagcta tcagactctc ctcatccaat taatctataa actatcctat
     2101 tatgagatca atttgccttt attttcaaat ccatcagcct tttcgattga ggacttatag
     2161 atttttcgac ataggtaatg attcaaatta ttatgatgaa tatcaaaatc gctttatcat
     2221 tcgcagagtt gccgaaaaat gctatttacc agcaaataaa ttgcttttaa ctctcattcg
     2281 aaggcatggc caagctttca gggtttgttt ctcgatttct gggactgcaa tagaacagtt
     2341 cgaaaaatat acccccgagg ttttggatag ttttaagcag cttgctgcaa ctgggcaggt
     2401 ggagttttta gccgaaactt atggacattc gcttgcaggc ttaaaaaaca aggaagaatt
     2461 taaaaggcaa atcctcatgc actcacaaag agtcgaagaa cttttcggga tcaaacctac
     2521 ttcattccgc aatactgagc ttatctattc ggatgaaatt ggagcaacca tagccgaaat
     2581 gggtttttac tctattctgg cagaaggggc aaaacatata ctgggatgga ggagtccaaa
     2641 ttatttgtat tacaatgcct tgaatcccag gcttagagtc ctgttacgta attttcgatt
     2701 aagcgacgac attggttttc gcttttcaaa caaagcatgg tcagaatggc cgcttacggc
     2761 cgaaaagtat gttagttggc tcaatgcctt cgatcccaat caggaattag ttaatatttt
     2821 tctcgattat gaaacttttg gtgaacatca ctggaaagaa actggaattt tcgaattttt
     2881 agaagcattg ccgaaaacca tactaaccca atcaaacttc gaatttcata ctccaaccga
     2941 aattgccagc ctacatcagc cggtagctcc cattcacgtt cctcatgcta catcctgggc
     3001 cgacgaagaa agagacctca ctgcctggct gggcaatgaa ctacaggaag aagccgtttc
     3061 gaacctctac gcgctggtat ctaaagttta cgcctgcgat gacccttcca tctggaaaga
     3121 ttggctctat ctccaaacca gcgatcattt ctattatatg tgtacaaaat ggttctccga
     3181 tggtgaagtt cacaaatact tcaatcctta tccttcgcct tacgaagcct atattaatta
     3241 tatgaatgtt ttatccgatt ttattattag agtagaaaag aattcggttg gaaaactcga
     3301 atatgaagga tggccttcac tgaaaagtgt atatgaattc aacaatattt ccggaccacc
     3361 tcttcctttt gtaactcaag cacctcattt tgttagaagc gataaaaaat catctaagtt
     3421 aatggcacaa ccttccgatt taattcattt tagtgatcga aagctgaaaa gtgcactaaa
     3481 aaatactgat tcggaattta tcgcaaaaat catagccaac tcggctgaag aagtaaccga
     3541 taaaattctt cgtaatctga ccaaacaaag aggagaagaa gttaaagaac tggctcatga
     3601 aattaaaaag gtttcgaaaa aggccttgag tgaggctgtt gaagaaatcc tcttgcatat
     3661 tcagaaataa tgttgcacta agggagtatc tttgttgtcg attcatttat caaacattcc
     3721 taactaaaag aattgcgatg gctgctaatg aaataataaa acccgattat ctttttgagg
     3781 tcagttggga agtatgtaac aagattggtg gaatttttac tgttttagcc accaaggctg
     3841 gtacaataaa aaaggaactg gatgataact atattgtaat tggacccgac gtatggatgg
     3901 aaacccatac gaatccgtat ttcgaggaag accacctttt gtacagagcg tggcgtatgc
     3961 aggcaacttc tgaaggattg aaagtaagac ttggtagatt taagggaaag aagggtgccg
     4021 tggctatttt ggtagatttc acttccttgt tccctcaaaa agacaaaata ttggctcatt
     4081 tatgggaaaa attcggactc gattccatta caggaggttg ggattacata gaacctctgt
     4141 tgtttggata tgcggctggg aaagtcatcg agagttttta cgaatatgaa gtattccctc
     4201 aggagaaagt agtcgctcat tgtcatgaat ggatgagtgg aggtacaatt ctttatttaa
     4261 aagaaaatct tccacaaatt gctacagttt ttaccactca tgccactgtt ttaggcagat
     4321 ccatttcggg gcataactgg cctttatacc gcaatctgga gttctataat cctgaaacca
     4381 tagcacgtga attaggtgtt gttgcaaaat attcactcga aaaaaccgct gctttgaata
     4441 ccgatattct tgcaaccgta agcaatattt cggcagccga agcgcgtaag tttttcggcc
     4501 gagatgtaga ttatattaca cccaatggtt tcgatgtttc attgattcca gattctgatt
     4561 tcgagaccca
//
LOCUS       DAOORU010000174         4521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_22826_length_4521_cov_4.947604, whole genome shotgun sequence.
ACCESSION   DAOORU010000174 DAOORU010000000
VERSION     DAOORU010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22826_length_4521_cov_4.947604"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="PLF20_09245"
     CDS             <1..1025
                     /locus_tag="PLF20_09245"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOX81222.1"
                     /translation="VSPEVTYEVMGIHEGIGRTLKISGVIPDFFNTFGLKLENGVIFS
                     EKEMEQSSPVCVIGSEIRAKIFPNKDPLGQYIKCSGIWLKVIGVLEYRPVSEKSSESL
                     GISNYNQHVYAPIKTVLNRIKDRSLITSKLLSGANVYNGGDFVVIESSSGAKAASFNQ
                     LDKIVVQVKESSMLSTSSEIIKRMLLRKHNEIEDFEVIIPELLLKQEQRTKDIFNIVL
                     GAIASISLIVGGIGIMNIMLASVLERIKEIGVRQAIGARRQDIVLQFLSEATLISIAG
                     GFIGIILGFILSKVIMKLAGILTIISPWSIIISFGVSAAVGIIFGYMPARKAALQDPV
                     TSLRYE"
     gene            1033..2523
                     /locus_tag="PLF20_09250"
     CDS             1033..2523
                     /locus_tag="PLF20_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOX81223.1"
                     /translation="MRKIIYLIFTILMGSLDILHVHSQGNVVRLSLEEVIQTASEQSP
                     DALMARHRFLSSYWEYRSYLAQFKPKLSLSATIPSLTKSMNSYTLPDGTETFVHQQYT
                     SYTGTLSLSQVIGFSGGSIYIQSGLQRLRNNLDSAGLSSWLSHPVTIGLRQPIYNFNS
                     YKWQRRIDPIRYLEATRRYIEDMEQISITVTRLFFNLIIAEGQLQISRINEANYDTLY
                     RIARGRFNMGTIAENELLQLELNLLKSRADAENARLDVENSEFILKSYLRLKGQEKIQ
                     LIPPSIPNQGSVSVSDAIEYARENRATALEFERRILEARRDLDQAKHEGRFQANLSID
                     YGLSKNGPNLSEVYKNLDDMQQLSFGVYVPILDWGQAKGRIKMAESNNELVKTAVEQE
                     SIDFEQEVYLAVMQYNMQKNQMEIAAKSDTVADKSYQVAKARYLIGKISITDLNIAQS
                     EKDLARMNFLRALQRYWNSYYTIRKLTHFDFTNNLPIRVDLNKIIE"
     gene            2615..3250
                     /locus_tag="PLF20_09255"
     CDS             2615..3250
                     /locus_tag="PLF20_09255"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOX81224.1"
                     /translation="MSVLVGKKAPLFYADAVINGSQFVEKFSLEQYIGKKYVVFFFYP
                     MDFTFVCPTEILAFQEKLKEFEDRNCAVIGCSVDSKFSHWAWLNTPKEKGGIQGVTFP
                     LVSDFSKTISENYDVLAGYYDYDVEDEVSTFIGEPVAYRGLFLIDKQGIVRHQIVNDT
                     PLGRNVDEAIRMVDALQFYEEHGEVCPANWHKGEEGIKATKEGIAEYLSKH"
     gene            3437..3790
                     /gene="rplS"
                     /locus_tag="PLF20_09260"
     CDS             3437..3790
                     /gene="rplS"
                     /locus_tag="PLF20_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004330114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOX81225.1"
                     /translation="MNKPELVKYVESLEEFKQFPEFKAGDTITVDYTIKEGNKTRTQR
                     FTGIVLQIKGTGNTKTFTVRKISGNIGVERIFPFASPNIERIEVNKRGAVRRARIFYL
                     RKLRGKKAKIKEKRS"
     gene            complement(4017..4139)
                     /locus_tag="PLF20_09265"
     CDS             complement(4017..4139)
                     /locus_tag="PLF20_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81226.1"
                     /translation="MKLVIILEMILSKIEEGGRGDEETGGCWRRLEDAGWREES"
     gene            4159..>4521
                     /locus_tag="PLF20_09270"
     CDS             4159..>4521
                     /locus_tag="PLF20_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81227.1"
                     /translation="MKKIWHCLYVISFVSGFQITLLAQSNISVALIIPDNYASIETRC
                     AKQFLQNLPDVNGFYLNPEDMVNNPNILDQIDVFWIHSNDSACLSQIAKNKVIWSKIN
                     KAVENGKGLLLSNNAVQLL"
BASE COUNT         1420 a          876 c          898 g         1327 t
ORIGIN      
        1 cggtaagtcc tgaagtaacc tatgaagtaa tgggaattca tgaaggcata ggaagaacat
       61 tgaagatcag tggagtaata cctgattttt tcaatacgtt tggattgaag ctcgaaaatg
      121 gcgtaatttt ttcggaaaag gaaatggaac aatcttcacc tgtttgcgta ataggttctg
      181 aaattagggc taagattttc cccaataaag atcccttggg acaatatata aaatgtagcg
      241 gtatatggtt gaaggtgata ggtgtattag aatacaggcc ggtttcagaa aaaagttcag
      301 aaagtctggg catcagcaat tataatcaac atgtatatgc tcccatcaaa accgtattga
      361 accgaataaa agatcgctcg ctcattacct ccaaactttt aagcggagca aatgtttaca
      421 atggaggtga ttttgtagtt atcgaaagtt caagcggagc aaaagcagct tcctttaacc
      481 aattagataa aattgtagtt caggtaaagg aaagcagcat gttaagcacg agtagtgaaa
      541 tcatcaagcg gatgcttctg cgtaaacata atgaaatcga agacttcgag gtaatcatac
      601 ccgaattgct tctcaaacag gaacaacgaa ccaaagatat cttcaacatt gtattgggag
      661 ccatagcgag tatttcatta atcgttggtg gaataggcat tatgaacatc atgctggctt
      721 cggttctgga acgtatcaaa gagattggcg taaggcaggc tattggagct cgcaggcaag
      781 acattgtact gcaatttctt tccgaagcta ctttgatcag catagcaggt ggtttcatag
      841 gaatcattct gggctttatt ttatcgaaag tcatcatgaa actggcagga attctgacga
      901 ttatatcacc ttggagcatc attatttctt ttggggtatc ggctgccgta gggatcatct
      961 tcggatatat gccggcacga aaggctgctt tacaagatcc ggtaacttca ctcagatatg
     1021 aataatatca tcatgcgaaa aattatttat ttgattttca ccattttgat gggatcatta
     1081 gacatcttac atgtgcattc tcagggcaat gtagtgagat taagcctcga agaagtaatt
     1141 caaactgctt cggagcagtc tcccgatgct ttaatggctc gacatcgctt tctgagtagt
     1201 tattgggaat atcggtccta tctggcccag ttcaagccca aacttagttt atcggctaca
     1261 attcccagtt taactaaatc tatgaattcc tatactcttc cggacggtac agaaactttt
     1321 gttcatcaac aatataccag ttatacaggt actttatcgc tttctcaggt aattggtttc
     1381 agcggtggaa gtatctatat acaaagtggt ttacaacgtc ttagaaacaa tctcgattcg
     1441 gcaggacttt catcctggct ttctcatcca gtaaccatcg gattgaggca acccatttat
     1501 aacttcaact cttataaatg gcagagacgt atcgatccta tccgctatct cgaagccaca
     1561 cgaaggtata tagaagacat ggaacaaatc tcgataacag ttaccagatt attcttcaat
     1621 cttatcattg ctgaaggcca attgcagatt tcaaggatta atgaagccaa ttacgataca
     1681 ttatacagaa tagctcgagg gagattcaat atgggaacca ttgccgaaaa cgagctcttg
     1741 caactggaac taaatcttct aaaatcgaga gccgatgctg aaaatgctcg tctggacgtt
     1801 gaaaacagcg aatttatact aaagagttat ttacgcttga agggtcagga aaaaattcaa
     1861 ctcattcctc cctctattcc taatcaaggc agtgtatctg tttcagatgc tattgaatat
     1921 gctcgagaaa acagggcgac ggctctggaa ttcgaaagac gaatccttga agctcgccgc
     1981 gacctcgatc aggcaaaaca cgaaggaaga tttcaggcta atctttctat tgactatgga
     2041 ttgtcgaaga acgggccaaa cctgtcggaa gtttataaaa acttggatga catgcagcag
     2101 cttagctttg gagtttatgt tcccatattg gattggggcc aggccaaggg aagaataaaa
     2161 atggccgaat cgaataatga actcgtaaaa accgcagttg aacaggaaag cattgatttt
     2221 gaacaggaag tgtatctggc cgtcatgcaa tacaacatgc agaaaaatca aatggaaatt
     2281 gctgccaagt ccgacactgt ggctgataaa agctatcagg tagccaaggc acgctatctc
     2341 atcgggaaaa ttagcatcac cgacctcaac atagctcaaa gtgaaaaaga cctggctcgg
     2401 atgaactttc taagagcctt gcaacgttat tggaatagtt attacaccat tcgcaagctc
     2461 actcacttcg attttaccaa taatctaccc attcgggtgg acttgaacaa aattatagaa
     2521 tagattggaa tcagcaacta aagtgtgata tacttggtta acaccattaa caataaatca
     2581 ataaacattt caataactta aaattaaatc gattatgtca gttttagtag ggaaaaaagc
     2641 cccattattt tatgcagatg cagtcataaa tgggagtcag ttcgtcgaaa aattttcgct
     2701 cgaacagtac attggtaaaa agtatgtcgt ctttttcttc tatccaatgg attttacctt
     2761 tgtttgtcca accgaaattc ttgcatttca agaaaaactg aaggaattcg aagatcgtaa
     2821 ctgcgctgta ataggatgct cggtcgattc gaaattttca cattgggcat ggttgaacac
     2881 accgaaagaa aaaggcggaa tacagggtgt aacctttcct ttggtttccg atttctcgaa
     2941 gactatttct gaaaattatg atgttttggc tggttattat gattatgatg tcgaagacga
     3001 agtttcgaca tttattggag aacccgttgc ttatcgcggt ctcttcctca tcgataaaca
     3061 gggcatcgta aggcatcaga ttgtcaatga tacgcctttg gggcgcaatg ttgacgaagc
     3121 aattcgcatg gtcgatgctc tccaatttta cgaagaacat ggagaggttt gtcccgccaa
     3181 ctggcacaaa ggagaggaag gaataaaagc aactaaagaa ggaatagcag aatatttgag
     3241 taaacactag cctttgcttc atttcctaaa aagcaggtga tacacattca tggagactcc
     3301 atatctttat ggacagttga tgcaacctga ttttttttca gaaagttttc ttgaaaactt
     3361 tatcgttgcc ttgtgagttg aaaaattgtt tgtatttttg cactcctttt ttaatgaaag
     3421 aaattttttt aacatcatga ataagcctga gttagtaaaa tacgtcgaat cgctcgagga
     3481 gttcaaacaa ttccctgaat tcaaggcggg agataccatc actgttgatt ataccatcaa
     3541 agaaggaaac aaaacccgta cgcagaggtt tacgggcatt gttttgcaaa taaaaggcac
     3601 aggcaataca aaaactttta ccgtacggaa aatttcaggt aacataggtg tggagcgcat
     3661 tttccccttc gcttctccca atatcgaacg tatagaagtc aacaaaagag gagctgttcg
     3721 acgcgccaga attttctatt tgagaaaact tcgtggcaaa aaagcaaaaa tcaaagaaaa
     3781 gcgttcttga tgcttgcttc caaatttgca cctaatgcat aatatacggg ccttcccaaa
     3841 ggtccttttt tattttatcc tataaattcg aagacggatc gttttttatt tgccatcagc
     3901 cttcagttta ttagatataa tggcaagggg cattcccttt cgctgattta cacacttttc
     3961 aaattacaaa atataaatta caaattacaa tatctccttt cctcctttat atacttttaa
     4021 ctttcttctc tccaaccagc atcctccagt ctcctccagc atcctccagt ctcctcgtct
     4081 cctcgtcccc cctcttctat tttactcaaa atcatttcaa gaattatcac taatttcatt
     4141 gcccgatatt ttaatgaaat gaaaaaaatt tggcattgtc tgtacgttat ttcgtttgtt
     4201 tcgggatttc agattactct tctggctcag tcgaatatta gcgtcgcctt gataattcct
     4261 gataattacg cttcgatcga gacgcgatgt gcgaagcaat tccttcaaaa cttgcccgat
     4321 gtaaatggat tttatttaaa ccctgaagac atggtcaata atccaaatat actcgatcaa
     4381 atcgatgttt tctggattca ttcgaatgac tcggcgtgtt tatcacaaat agccaaaaat
     4441 aaggttatat ggagcaaaat aaacaaggcc gtcgaaaatg gcaagggttt gctcttgagt
     4501 aataatgctg ttcagcttct a
//
LOCUS       DAOORU010000175         4440 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_23365_length_4440_cov_5.803193, whole genome shotgun sequence.
ACCESSION   DAOORU010000175 DAOORU010000000
VERSION     DAOORU010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23365_length_4440_cov_5.803193"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="PLF20_09275"
     CDS             complement(<1..1141)
                     /locus_tag="PLF20_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOX81228.1"
                     /translation="MKYDPSKKIFELLQFGEFGDVNPSITDSATFTFMQAKTMSDTFA
                     GKTEGCFLYSRHWNPSNKYLADALAAMEGTESCWVTASGMAAITITLLHLCNSGDHIV
                     SSLCTYGGTFAFLHNYLAKFNISVSFVDITRPEEVKAAIKPNTKLVYTETMTNPLLQI
                     SDISLLAEIANQHGIPLVVDNTFTPMIVSPALLGAHIVVYSLTKFVNGKNDCVAGAIC
                     ASKDFIQSLSNVNDGSAMLLGPVLDPLRSSSILKNLHTLHIRMQKHSSNAMYLARKFR
                     ETGLKTIYPGFEDDPSYPVFNKIMNPDYGFGGMLAVDLETSEKAAAFMEKMELAGVGY
                     LAVSLGYFKTLFSNSGKSTSSEVPPEYQQKMGLSEGLVRFSVGLDH"
     gene            1286..1759
                     /locus_tag="PLF20_09280"
     CDS             1286..1759
                     /locus_tag="PLF20_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOX81229.1"
                     /translation="MHAEFQLDSLDRKILNILIRNARTPFLEIARECGVSGAAIHQRV
                     HKMERSGIIQNATLKLSPKALGYLTCAFIGIQINLTTTRTHEEVFEKIKQIPEIVECH
                     HITGKYSLLAKIYTRNNEHLKQIIVDKIQSIPEITFTETFISLEEGFMRSFIPEA"
     gene            complement(1819..4224)
                     /locus_tag="PLF20_09285"
     CDS             complement(1819..4224)
                     /locus_tag="PLF20_09285"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOX81230.1"
                     /translation="MKKFSISIAVLLLPLVSKASEADLLIPDLTSRFFNGQIEGRHLL
                     LYFSIIILFGLMFGVWQFARIKRLPVHRAMLEISETIYTTCKTYLLQQGRFLLILLVI
                     ISIIIFYYFFGLSGLSVGKVLIILLWTVLGILGSYSVAWFGIRINTLANSRTSFASLR
                     QKPWNVLSIPLQAGMSVGLFLICIELIMMLIILLAVPGDSAGACFVGFAIGESLGASA
                     LRIAGGIFTKIADIGADLMKIVFSLPEDDPRNPGVIADCTGDNAGDSVGPTADGFETY
                     GVTGVALISFIVLAVTNVEFQSTLIVWIFMMRILMVLTSLLSYGINQAISAKQYKKAK
                     EFDFETPLSRLIWITSGVSIIITFLTSYFLLNDLTTPEGIPLTNLWWQLATIISCGTL
                     AAAIIPELTKSFTSSKSRHVNEIVTASKEGGPSLTILSGMVAGNFSAFWKGLSMVALM
                     FVAYFTTLGFEQPIFGIYPGIFAFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVFELS
                     RIEQIPGIEKEIEQDFGFTPDFELGKHYLEANDSAGNTFKATAKPVLIGTAVIGATTM
                     IFSIILILAKSGLMALQLTDPMVILGFIAGGAVIYWFSGASMQAVTTGAHRAVEYIKR
                     NIRLDKEVADVEDSKEVVRICTRYAQYGMWNIFAVIFSLTLAFAFFDPNFFVSYLISI
                     AVFGLFQAIYMANAGGAWDNAKKVVEVDLKQKNTDLHAATVIGDTVGDPYKDTSSVAL
                     NPIIKFSTLFGLLAAEISIHITLAAKNTGTLNYAPWIGAIFLVIGLFFVHRSFYKMRI
                     KKE"
BASE COUNT         1431 a          907 c          918 g         1184 t
ORIGIN      
        1 cgtgatcgag gcccaccgag aagcgaacta agccttcgga gagccccatt ttttgttgat
       61 attctggagg gacttcactc gatgtacttt tccctgaatt actgaaaaga gttttaaaat
      121 atcccaagct cacggccaga tatcccaccc cggccaattc catcttttcc ataaaagcag
      181 cagctttttc gctggtttct aaatcgacgg ccagcatacc tccaaaacca taatcaggat
      241 tcatgatttt attgaatacc ggatagctgg gatcgtcttc gaagccggga tagatggttt
      301 tcaaaccggt ttcacgaaat tttcgggcaa gatacatggc attgctgcta tgtttttgca
      361 tacggatatg caatgtatgg aggtttttca aaatagaaga tgagcgtaag ggatcgagaa
      421 cagggccgag aagcatggca cttccgtcgt ttacattgct cagggattga ataaaatcct
      481 ttgatgcaca gatagctcca gccacacaat cgtttttccc gttcacgaat ttggtgaggc
      541 tatacaccac gatgtgtgca cctaacaagg cggggctcac tatcatggga gtaaatgtat
      601 tgtcgacgac gagtggaatg ccatgttgat tggcgatttc ggcaagtaat gaaatatcgc
      661 taatttgcaa caacggattc gtcatggttt cggtatagac aagctttgta ttgggcttga
      721 tggcggcttt tacttcttcg ggccttgtaa tatccacaaa gctgacacta atgttgaatt
      781 ttgcgagata attgtggaga aaagcaaaag ttccgccata agtgcaaagg ctggagacaa
      841 tgtgatcgcc cgaattgcat aaatgaagta atgttatagt aattgccgcc attccactgg
      901 ctgtaaccca gcacgattct gttccttcca tggctgcgag ggcatctgca agatatttat
      961 tacttggatt ccaatggcga gaatacagga aacagccttc ggttttcccg gcaaaggtgt
     1021 cactcatagt tttagcctgc ataaaggtaa atgtagcaga atcggtaatg gaggggttaa
     1081 catcgccaaa ctccccgaac tgaagaagtt cgaatatttt ctttgaagga tcgtatttca
     1141 tgatgataca attaaagtaa gttttcacaa aattatgaaa aagacatgta ttcgtgcaga
     1201 ttttaaaaat tttttatcat ttgattaata actttagcga aatttcgatt aattttgtaa
     1261 aagtgtaagt aaaaaggaag aaaatatgca tgctgagttt caactcgata gtttagaccg
     1321 caaaattttg aatattttga ttcgaaatgc cagaactccc tttctcgaaa ttgcgaggga
     1381 atgcggagta agcggtgcag cgattcatca gagagtccat aaaatggaaa ggtcaggaat
     1441 cattcaaaat gccactttga agctttcacc caaagctctg ggttatttaa cctgtgcctt
     1501 cattgggatt caaatcaatc ttactactac tcgtacacac gaagaggttt tcgagaaaat
     1561 aaaacaaata cccgaaatcg tggaatgcca tcatattacg ggaaaatata gtctgttagc
     1621 caagatttac actcgtaata atgaacactt aaaacagatt atcgtcgata agatacaatc
     1681 tattcctgag attactttca cagaaacttt tatctcgctc gaagaaggct tcatgcgtag
     1741 tttcatacca gaagcctgaa attttaaacg atgattttca aaatcgaatt cgcgaaaagc
     1801 cagaggaaac tgattcggtt attccttttt tatgcgcatt ttatagaatg aacgatgaac
     1861 aaaaaataat ccaatcacta gaaaaattgc accaatccat ggagcataat tcaaagtgcc
     1921 tgtatttttc gcagcaagtg taatatgtat ggagatttcg gctgcaagca aaccaaaaag
     1981 agtagaaaat ttgattatag gattgagggc cacactcgag gtatccttat aggggtcgcc
     2041 cacagtatca cctatgacgg ttgcggcgtg caggtcggta tttttttgtt tcaggtcgac
     2101 ttcgacaact tttttagcat tatcccaagc tccacctgca ttagccatat aaattgcctg
     2161 aaacaatccg aacacggcga tagatatcaa ataagaaaca aaaaagttag gatcgaagaa
     2221 agcgaaggct aaggtaaggg aaaagatgac tgcgaagata ttccacatgc catattgtgc
     2281 atatcgagtg cagatgcgca ccacttcctt gctgtcttcc acatcggcta cttctttgtc
     2341 gaggcgaatg ttacgtttga tgtattctac ggcacgatgg gctccagtag tcactgcctg
     2401 catcgaagct ccgctgaacc agtaaattac cgctccaccg gctataaaac ccaaaataac
     2461 cataggatcg gtaagttgca aagccatcaa tccactttta gccagaatta aaatgatgga
     2521 aaagatcatg gtggtagctc cgattactgc agttccaatt aaaacaggct tggccgtagc
     2581 cttgaaggta ttgccagccg aatcgttggc ttcgagataa tgcttgccga gttcgaaatc
     2641 aggtgtaaaa ccaaaatcct gttcgatttc cttttcgatg ccagggattt gttcgatacg
     2701 tgaaagctca aaaacacttt gtgcgttgtc ggtcacgggt ccatagctat ccactgctat
     2761 cgtcactggt cccattccca aaaatccaaa agccaccaaa ccaaaggcaa aaatacctgg
     2821 ataaatccca aaaataggct gctcaaagcc taaagtagta aaataggcaa caaacatcag
     2881 agcaaccatg gaaaggcctt tccagaatgc gctgaaatta ccagccacca tgcccgataa
     2941 aatcgtcaat gatggtcctc cttccttcga agctgtaaca atttcgttta catgccttga
     3001 tttgctgctt gtaaaactct tggtaagttc ggggataatg gctgcagcca aagttccgca
     3061 cgaaatgatg gtagccagtt gccaccataa atttgttaat ggaatgcctt cgggggtagt
     3121 aagatcattc aagaggaaat aactggtaag gaaggtaatg atgatagaaa ccccagaggt
     3181 aatccagata agcctcgaaa gtggcgtttc gaagtcgaat tctttggctt ttttatattg
     3241 tttcgcactg atagcctgat tgattccata gctcaagagt gaggtaagga ccataagaat
     3301 tcgcatcatg aatatccata cgatgagggt agattgaaat tctacgttgg taacggctaa
     3361 aacgataaaa gagataagag ccacgccagt tacaccataa gtttcgaatc catcggcagt
     3421 agggccgacg ctgtcgcccg cattatcgcc agtgcagtca gctattaccc ctggatttcg
     3481 agggtcatct tccggaaggc tgaatacgat cttcatgaga tctgcaccaa tatcggcgat
     3541 cttggtaaat atcccaccag ctatacgaag agccgatgct ccaagagatt caccaatggc
     3601 aaaacctaca aaacaagctc ctgcactatc acccggaaca gctaataaaa taatgagcat
     3661 cataatgagt tcgatacaga taagaaacaa gcctacactc attccagcct gcaaaggaat
     3721 tgaaagtaca ttccatggct tctgacgcaa tgaagcaaag gaagtacgac tgttggccag
     3781 agtgtttatt cttatcccaa accacgcaac actgtatgat cctaaaattc cgagcactgt
     3841 ccataataga atgatcagga ctttccccac ggataaacca gaaagaccaa agaaataata
     3901 aaatataatg atagaaataa taactaatag aatgagaagg aaacggcctt gttgaagaag
     3961 ataagtttta caggtggtat agatggtttc cgaaatttcg agcatagcac gatgtacagg
     4021 taaccgttta attcgggcaa attgccaaac cccaaacatc agtccaaaaa gaatgattat
     4081 cgaaaaataa aggagcaaat gcctcccttc gatttgccca ttaaaaaaac gagatgttag
     4141 gtccggaata aggagatcgg cttcacttgc cttcgaaacc aacggtaata gcaataccgc
     4201 tatgctaatg ctaaattttt tcataccatc ttcttcttat tttttggcaa aattaagaag
     4261 caaatttcta atgcaaacga ttgcgtaaaa ttttacggca gttctaaaat caaggatttg
     4321 aatatcattt cataagatat taaaaaaatc attactgacc gacaaaaaaa ttgaataaaa
     4381 tgaaagaggg acaaaaccga agtttcatcc cccagttgta aatttgtgta gtaaacctaa
//
LOCUS       DAOORU010000176         4284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_24432_length_4284_cov_5.001892, whole genome shotgun sequence.
ACCESSION   DAOORU010000176 DAOORU010000000
VERSION     DAOORU010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24432_length_4284_cov_5.001892"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..2023)
                     /locus_tag="PLF20_09290"
     CDS             complement(68..2023)
                     /locus_tag="PLF20_09290"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOX81231.1"
                     /translation="MKKHTIGLLALIFSFLSSGLSAQNLYIKTDSLQKALPSLKGVEH
                     REAMRQIIEYSEEFSKDISKEYSLRLFEEAKMVGDTFYMIEGLYNLGMVEKLDGNFFQ
                     ANQYLNELISLAKKMKNPEFQAKNLTRVSIILSLMDMSDLAFTYLEQAIVLLRNSSDI
                     REIVNLMQTIAQEYYRLRLYHEAIGIYRILKIFLIPQIPANDLSRTIFYRDYAYALCM
                     NDYLTDALYYADKAREIAMKNHSLRDIYNLDRLYGLIYNKMQNNDKVIQYLEPAALQT
                     ENYGYLLKSSYIHNELAHYLQQAGDYSHALEHQLKALRLRQKANFNSVICFAYINYGD
                     ALMKAGKMDSAFIYLSMGKDLASKMNFKTEIRRAYEKLGDYYMRTNRFDKALAFIKEA
                     RDIQDKEAAIQSEQQRKLLALNTEAQSYNEKNLGLKEIESKQNIFFILIAGIILLLIV
                     ILWLYYINHRREDFLRSIEIKQKILASQLNPTFIFNMLVGIRGIIRQGRNEEAGKYLA
                     GFAKLIREILVSSQHNYQFIEREIDMLNSYLELQKIRFSGLLNYEIHLSQSLDSSRMG
                     IPPFLCHSILENFFNFIADKNDKPFHIFIDFQEDNNSIVETIKINLDSEIYHQNLGSL
                     WLDELKPVLELIKERITIICKSKKNHY"
     gene            complement(2020..4047)
                     /locus_tag="PLF20_09295"
     CDS             complement(2020..4047)
                     /locus_tag="PLF20_09295"
                     /inference="COORDINATES: protein motif:HMM:NF018305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOX81232.1"
                     /translation="MLLLIFEPLQADDVRGMFFYHQRLDDLRQNITKAKNPEEKIRNY
                     LLLSKMYQIISADKASAYADSAIFLSHSAELHSLEADALLLKARVYMIGYVTDSANLF
                     LDRAYNKALKCKAYSSLGYILTTQAIKSMYENDKGVFLDEINQAEKYFKLAEDYEGLV
                     YAYNTRASLYISLNLYEEARSYIRSAMEEADKLNDQSFKIPIQLKQADLLSSTGNHQQ
                     AISLILLNIPYLKKNNLVYLEISSLRSISSLYIKLGEYNKALEYAYDAYDKCKPLGFH
                     LLASDLLTLIGNIFHRTNNYDLSIRMNHQARTLRYYNHFPLACFNSYYNLGALFGAIN
                     QIDSSLYWLNQAMALANKYNNIETKALTAELFYQTYESAGLYEPALNYFQLWSEYKAQ
                     RNQTVTKNAFALQATLDVQRQKEQYELNTKRIQTRLIFSYLLAGLTLLVIIIALLLIR
                     SYKNKIKVQYSQMRERLLRMQMNPHFIFNALIAIQSYIYKNDTSKAIGFLDDFSNLIS
                     LFLTSSSKDFIPLSEELSIIRYYLEIQQLRFDDKFEYEIEVSPELKTEKIAIPPLLIQ
                     PFIENSIEHGFNDIKIKGHLKIVYTRQGKNLMIYILDNGIGLSRSRDQNPDPHKHKPM
                     AIPITKERLEILNRRFRREQIQFKVTDNLTSGSVYPGTRVEFSIPLIDWKT"
BASE COUNT         1302 a          756 c          827 g         1399 t
ORIGIN      
        1 aaaacttaat cttcatctcc tctgaaaaag ttgcatttat tagttgaatt tagtagaatt
       61 ttgtcggtta atagtgattc tttttcgatt tgcaaataat ggtaattctt tccttgatga
      121 gctcgagaac aggtttcaac tcatcgagcc atagcgatcc gagattttga tgatatattt
      181 cgctatcgag atttattttt atagtttcca cgatactgtt attatcttct tgaaaatcaa
      241 tgaaaatatg gaagggtttg tcgtttttgt cggctataaa gttgaagaag ttttcgagaa
      301 tggaatggca taaaaacggt ggaattccca tacgagaaga atcgagtgat tgactgagat
      361 gaatttcata gttaagtagc ccagagaaac gtattttttg gagttcgaga taactattca
      421 acatgtctat ttctcgctca ataaattgat agttgtgttg ggagcttacc agtatctctc
      481 gaatcaattt ggcaaaacca gccagatact tccctgcttc ttcgtttctt ccttgccgga
      541 taatgccacg aattcccacc aacatattaa aaatgaacgt aggattaagc tgcgatgcaa
      601 ggattttttg tttgatttcg atacttctta aaaaatcttc gcgtctgtga ttgatatagt
      661 acaaccacaa tataacaatc aatagtaaaa taattcctgc aatcagaata aagaaaatat
      721 tttgcttcga ttctatttct tttaatccga gatttttctc gttataggat tgagcttctg
      781 tatttaaagc caagagttta cgttgttgtt ccgattgaat ggctgcttcc ttatcttgaa
      841 tatctctggc ttctttgata aatgccagag ctttatcgaa tcgattcgta cgcatgtaat
      901 aatcccctaa tttttcataa gctctcctta tttctgtttt aaaattcatt tttgaagcca
      961 aatctttccc catcgaaaga taaatgaagg ccgaatccat tttgccagct ttcatcaagg
     1021 catcgccata atttatatag gcaaagcaaa tcaccgagtt aaaattagct ttttgccgca
     1081 agcgcaatgc tttcaactga tgttccaatg catgggaata atctcctgct tgttgcaagt
     1141 agtgggcgag ttcattatgg atataagagc tcttcagaag gtaaccataa ttttcggtct
     1201 gcagggcggc cggttcaaga tattgtataa ccttgtcatt attttgcatc ttattataaa
     1261 tgagtccata aagtctgtct aaattgtaga tgtcccggag ggaatgattt ttcatggcaa
     1321 tttcacgagc cttatcagca taatacaggg catcggtaag ataatcgttc atacatagtg
     1381 cataggcata gtccctgtaa aagatggttc ttgacaaatc attggcggga atttgaggta
     1441 ttaggaatat tttcaatatt ctataaattc ctattgcttc atggtaaagt ctcaggcggt
     1501 aatattcttg ggcaatggtt tgcattagat tgactatttc acgaatatca gacgaatttc
     1561 tcaataaaac gatagcttgt tccaaataag tgaaggccag atctgacatg tccattaagg
     1621 aaaggattat tgagactctc gtaagatttt tcgcctgaaa ctcaggattt ttcatttttt
     1681 tggctaagga aatcaattcg tttaaatact ggttcgcctg aaaaaaattc ccatccaatt
     1741 tttcgaccat ccccaaattg tacaagccct caatcatgta aaaggtatcg cccaccattt
     1801 ttgcttcttc gaacaatcga agcgaatatt ctttggaaat atctttgctg aattcttccg
     1861 aatattcaat aatttgcctc atagcttccc tgtgttcgac tcctttcaaa ctgggcaatg
     1921 ctttttgcag gctatctgtc ttaatataaa gattctgagc agaaagcccc gaagagagaa
     1981 aagaaaaaat caaagccaat aggccaatag tatgtttctt catgtcttcc aatcaattaa
     2041 aggaattgaa aattctaccc tcgtgcctgg atagaccgaa cctgaggtaa gattatcggt
     2101 aaccttgaac tgaatttgct cacggcgaaa tcggcggttc aagatttcaa gacgctcctt
     2161 ggtaatggga atggccatgg gtttatgttt gtgagggtcg ggattctgat ccctgcttct
     2221 tgacaaacca ataccattgt ccagtatgta tatcatgaga ttctttcctt gccgtgtata
     2281 gactatcttc agatgacctt taattttgat gtcattaaat ccgtgttcta tactgttttc
     2341 gatgaatggt tgaataagta agggtggaat ggctattttt tcggttttga gctcaggcga
     2401 aacttctatc tcgtattcga atttatcgtc gaatcgaagt tgctgaattt cgagatagta
     2461 ccgtattatg gacaattctt ccgacaaggg gataaaatct ttcgacgaag aagtcaaaaa
     2521 cagactgata agattcgaaa aatcatcgag aaaaccaatg gccttcgaag tatcattttt
     2581 atagatatag ctctgtatgg ctattaaagc attgaagatg aaatgagggt tcatctgcat
     2641 gcggagtaat ctttcacgca tctggctata ctgcactttg attttattct tatatgaacg
     2701 tataagaagt aaggcaatga tgataaccag gagcgtaagg ccagccagaa gatatgaaaa
     2761 gataagccga gtttgtattc gtttggtgtt cagttcgtat tgctcctttt gcctttggac
     2821 atcgagtgtg gcttgcaggg caaacgcatt tttggttact gtttggtttc gttgggcttt
     2881 atattccgac cagagttgaa aataatttaa ggcaggttcg taaagcccgg cactttcata
     2941 ggtttgatag aacaattcgg ctgtcaaagc cttcgtttct atgttgttgt atttattagc
     3001 caaggccatg gcctgattca accagtaaag agaactatct atttgattta tagccccgaa
     3061 taaagcaccc agattataat aggaattgaa acaggccagc gggaaatgat tgtagtaacg
     3121 caacgttctt gcctgatgat tcattcgtat actgagatca tagttgtttg tccgatgaaa
     3181 gatgttccct attaaagtga gaagatctga agccaggaga tgaaagccca atggcttaca
     3241 tttgtcgtaa gcatcatagg catattccaa agccttatta tattctccta attttatgta
     3301 aagagaggaa attgagcgta aactgctgat ttctaaataa acgaggttat ttttcttgag
     3361 atagggaata ttcaataaga taagactaat ggcttgctga tgattacccg tcgaagaaag
     3421 taaatcggct tgtttgagct gaatgggaat tttaaacgat tgatcattca atttgtctgc
     3481 ttcttccatt gcagaacgaa tgtaactacg ggcctcttca tacaaattca aactaatata
     3541 taaacttgct cttgtattat aagcgtatac aaggccttcg taatcttcgg caagtttgaa
     3601 atatttttca gcctggttga tttcgtcgag aaatacacct ttatcatttt cgtacatact
     3661 tttaatggct tgggtagtca aaatatatcc cagggatgaa taagccttgc atttcaaagc
     3721 tttattgtat gccctatcga gaaataagtt agctgaatcg gtcacatacc caatcatgta
     3781 aactctggcc ttcaacaaga gagcatcggc ttcaagggaa tgcaattctg cagaatggct
     3841 caaaaaaatt gccgaatcgg cgtaagcaga agctttatcg gccgaaatta tctggtacat
     3901 cttcgaaagc agcagataat ttctgatttt ctcctcagga tttttcgctt tagtaatatt
     3961 ttgtcttaag tcatcgagtc gttgatgata gaaaaacatg cctcttacat cgtcggcctg
     4021 aagaggctca aatataagca gtagcatcaa aatcctaaag aaatgaaaaa ttcttttcac
     4081 attgaatgca ttaatatagc attttctcat gaaatgaatc atacgagaac caattttgga
     4141 gtaaaccttt caaagataga atttttatag aattacaaat atcgttgata aagatttatt
     4201 gatagcatgg atgttatcta tttaatcatt actgaccgac aaaaaaattg gataaaaaga
     4261 aggagggaca aaaccgaagt ttca
//
LOCUS       DAOORU010000177         4247 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_24727_length_4247_cov_4.409113, whole genome shotgun sequence.
ACCESSION   DAOORU010000177 DAOORU010000000
VERSION     DAOORU010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24727_length_4247_cov_4.409113"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLF20_09300"
     CDS             <1..396
                     /locus_tag="PLF20_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOX81233.1"
                     /translation="LHEKQNPYLSCPSGAPMVQHSLSAMFEMAFQRKLELTRVVEKMC
                     HAPALLFNIHKRGFIREGYFADLVLIDPNEPWEVNSSNILYKCGWSPMEGTHFSSRVT
                     HTWVNGHLVYKKGLFDESRKGMQLMFDNR"
     gene            400..858
                     /locus_tag="PLF20_09305"
     CDS             400..858
                     /locus_tag="PLF20_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81234.1"
                     /translation="MKKQIYFFWMLLAVLFTSCGRKTVNIVKEEFEDGSPKVVRQYEI
                     RGKDSILLRETIFYQNGQKYIEGKYTNNQRDSIWTAWLPDGKIWSQGGYKEGLEDGPK
                     KVFYTNGSLFYEGEHKMGKRIGVWKFYDSVGNLIQSIDYSSIISSDTLPH"
     gene            complement(944..1819)
                     /locus_tag="PLF20_09310"
     CDS             complement(944..1819)
                     /locus_tag="PLF20_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81235.1"
                     /translation="MKRAIIALVVLFALAGCNSHKKELEKLKAKNDSLMQVTQLKEES
                     LYEYITSFNEIQRNLDSIKALENIINIRTAGGELKNSAKDQINEDIKTIYELLVKNKK
                     LVASLQKKLNSSDVRMAELEKMVTYLNTQVEEKDAQINALKNELGNMNIKVENLSSQV
                     AVLEEVTRQKEEEIQKHKDEIESKTTLLNTAYYAIGTRKELTENNIINKEGGFLGIGS
                     TKTLKGDFNKDYFTKVDITRLEYIPLGAKKARLITKHPASAYRISGVKRADTLFITNS
                     SEFWAAGKYLVIEVE"
     gene            complement(2264..>4247)
                     /locus_tag="PLF20_09315"
     CDS             complement(2264..>4247)
                     /locus_tag="PLF20_09315"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX81236.1"
                     /translation="TGNVYTVAAIVNATGYSWTVPSGATITAGANTNTITVSYGASAV
                     SGNVSVYGTNSCGSGASSSLAVTVNPRPVPTISGPATVCAGTSGNVYTTQTGMSNYTW
                     TVSAGGTITAGQGSNSVTVTWNTAGGQNVTVTYTNAAGCSASTPATYNVTVNALPTPT
                     ISGNTNLCQGSTGVNYTTQSGMTNYVWAVSSGGNITGGQGTNAITVNWNGSGAQTVSV
                     NYINASGCTASSPSVLNVTVNPTPTPSINGPANPCLSPNYYDYFTEAGMSNYQWTVSS
                     GGSIYSGQGTANLRVIWNTTGLQSISVSYVSPQGCMMPGPYSKTIFVQPLPNAAGAIT
                     GSGEVCAGESGVVYSVGNILNATSYQWTVPGGATIVSGQGSNTITVNFASNAISGEVS
                     VAGMNGCGLGVPSFLAVIVKPLPGVAGTIAGHPNVCQGATNVTYSVAPIANASGYVWS
                     LPVGASITAGANTNSIKVSFSENAVSGNISVYGTNACGNGISSPEFFVTVNPIPPTPT
                     ITVSNYTLTSSAPEGNQWYLNGVPIPDATGQSYTATETGNYYVIVTLNGCSSAPSNTI
                     LVIIEGMISLDHNSFKVYPIPNDGRFTIELVLQKSGIYTLEIFNSLGLRVYSSEIYVD
                     GKHILPMDLSYLPSGVYTVTLFNTQLNAVRRIVISK"
BASE COUNT         1266 a          923 c          778 g         1280 t
ORIGIN      
        1 cttcatgaaa aacagaatcc ctatctgagt tgtccgtcag gagcgcctat ggtacaacat
       61 agtctttccg ctatgttcga aatggcattt caacgtaaac ttgagcttac aagagttgtg
      121 gaaaaaatgt gtcatgcacc ggctctactt tttaatattc ataaaagagg ttttatacgg
      181 gagggttatt ttgccgatct cgtcctaatc gacccgaatg aaccatggga ggtaaattca
      241 agtaatattt tgtataaatg cggctggtcg cccatggaag gaactcattt ctcctcacgg
      301 gttactcata cctgggtaaa tggacacctg gtttataaaa aagggttgtt cgacgaaagt
      361 cgaaaaggaa tgcaattgat gtttgataat cgttaaaaaa tgaaaaaaca aatctatttt
      421 ttctggatgt tattggcagt tctattcact agctgtggcc gtaaaactgt aaacattgtg
      481 aaggaagaat tcgaagatgg ttctccaaag gttgtgcggc aatatgaaat tcgaggaaaa
      541 gacagcattc ttctccgaga aaccattttt taccagaatg gacaaaaata tatagaaggg
      601 aaatatacta acaatcagcg cgacagtata tggactgcct ggcttcccga tggaaaaatt
      661 tggagtcaag gagggtacaa ggaaggcttg gaagatggac ctaaaaaagt attttatact
      721 aatgggtctc ttttttatga aggtgagcat aaaatgggaa agcgcatagg tgtatggaaa
      781 ttctatgact cggtaggaaa tctgattcaa agtatcgatt atagttcgat cataagcagt
      841 gataccttgc ctcactaaag cccgtattta ccagcaaaag aataaaataa ccggataagt
      901 ataataacca cttatccagt ttttgagtgt atgtattgat tttttattct acctcgatga
      961 ccagatattt acctgcagcc caaaattcgg aagaattagt aatgaaaagg gtatctgcac
     1021 gcttcacccc actgatacga taagcagagg caggatgttt ggtaataagt ctggctttct
     1081 ttgctcctaa aggaatatat tccaaacgcg taatatctac cttggtaaaa taatccttat
     1141 tgaagtctcc tttcaaagtc tttgtggaac ctattcccag aaaaccacct tctttattga
     1201 tgatattatt ctccgtcaat tcttttctcg tacctattgc ataataagct gtatttaata
     1261 aggtggtctt gctttcgatc tcatccttat gtttctgaat ttcttcttct ttctggcgag
     1321 ttacttcttc gagcacagct acctgcgacg aaagattctc gaccttgatg ttcatatttc
     1381 ccagttcatt cttcagcgca ttgatctgag cgtctttttc ttcaacttga gtattcaaat
     1441 aagttaccat tttttcgagt tcggccatcc tcacatcgct gctattgagt tttttctgaa
     1501 gcgaggcgac tagtttctta ttcttcacca gtaactcata aatagtctta atatcctcat
     1561 tgatttgatc ctttgccgaa ttctttagtt cacctccagc ggttcttata ttgatgatgt
     1621 tttccagtgc cttgatcgaa tcgagattcc tctgaatttc attaaaagaa gtaatgtatt
     1681 cgtaaagcga ttcttcttta agttgagtta cttgcatcag actatcattt ttagccttga
     1741 gcttttccaa ctccttttta tgactattgc aacctgccag ggcaaacaac acaactaaag
     1801 caattattgc acgtttcatg gcgatttggt ttaaatattt aacatttatt aacttcttga
     1861 taacgggttt catgctattt tattataata caatattatc catctttaat cggacaatat
     1921 gagttttcaa cacaagactt attctattcc taaggattta aaaagtttgg gctacaaaat
     1981 tacggcaatt ctttctataa acattatgtg atatataact catctcgagt aatatatcga
     2041 aatactttcg agtcgattca atttatttat ataagcaaaa agcagaaatt tcgtggcttt
     2101 aaaatcccag acaagcaaac cacgataaca caatatttac aagaaacggt ttacttttta
     2161 tttctttgtc atgttagcta ttcattaaaa cggccggata aaaaaactct ggcctgcggc
     2221 agagcacaag ccagagtgta taggacttta gagcaaatct gccctatttg ctgatcacga
     2281 ttcttctcac tgcattcaac tgcgtattga acaaagttac cgtataaaca cccgaaggca
     2341 gataactcaa atccatcggc aaaatatgtt tcccatcgac ataaatctca ctgctataaa
     2401 ccctcaatcc taaactattg aagatttcta aagtatagat tcctgatttc tgcaacacta
     2461 actcgatggt aaatctccca tcattgggaa taggataaac cttaaatgaa ttatgatcga
     2521 gcgaaatcat gccttctata attaccaaaa tggtattgga aggtgctgaa ctacaaccat
     2581 tcaaggttac aattacatag taattgcccg tttccgttgc agtatagctt tgtccagtag
     2641 cgtccggaat gggaactcca ttcaaatacc attgattgcc ttccggtgca ctgcttgtta
     2701 aagtataatt cgacaccgta atggtaggag taggaggaat gggattcaca gtaacaaaga
     2761 attcgggtga agaaatacca ttgccgcaag catttgtccc atataccgaa atattaccag
     2821 aaacagcgtt ttcactaaaa cttaccttga tggaattggt atttgcacct gccgttatgc
     2881 ttgcacccac cggcaaactc catacataac ccgaagcatt agctatagga gcaactgaat
     2941 aggtaacatt tgtggcaccc tggcatacat tcggatgccc agcaattgta ccggctactc
     3001 ctggtaaagg tttcacaatt acagccaaaa aagaaggcac acctaaacca caaccattca
     3061 tccctgctac acttacttcg ccagaaatgg cattcgaagc aaaatttacc gtaatagtgt
     3121 tgcttccctg accagaaact atggttgctc ctcctggaac agtccattga tatgaagtag
     3181 cattgagaat attccctact gaataaacta ctccgctttc cccagcacaa acctcacctg
     3241 aaccagttat tgctcctgcc gcattaggca aaggctgtac aaatatcgtc ttactatatg
     3301 gccctggcat catgcacccc tgtggactta catacgaaac actgatggat tgtaatcccg
     3361 tcgtattcca tattactctt aaattcgccg tcccctgacc actatatatc gacccacctg
     3421 aagaaacagt ccactgataa ttgctcatac ctgcctcagt aaaataatca taataattcg
     3481 gactcaaaca gggattagcc ggtccattga tagaaggcgt cggagtagga tttaccgtaa
     3541 cattcaaaac actcggactc gatgccgtac atcccgaggc atttatgtaa ttcacactta
     3601 cagtttgtgc accactacca ttccaattca ctgttatagc attcgtacct tgacctccag
     3661 taatattgcc acctgacgat actgcccaaa cataattggt catccccgac tgtgtggtgt
     3721 aatttactcc tgtacttcct tggcacaaat tagtattacc cgaaatggtt ggcgttggca
     3781 aggcatttac cgttacattg tatgtagccg gtgtacttgc agaacatccc gcagcatttg
     3841 tatatgttac cgtgacattc tgccctccag ccgtgttcca tgttaccgtt accgaattgc
     3901 tcccctgacc agctgtaata gtaccacctg cagatacagt ccatgtgtag ttgctcatgc
     3961 ctgtctgagt agtgtataca ttcccagacg tacctgcaca aacagtcgca ggaccactaa
     4021 tggttggaac tggcctcgga tttacggtta cagccagaga tgaagaagct ccactcccac
     4081 aactatttgt gccgtatact gaaacattcc ctgaaaccgc actggcacca taacttacgg
     4141 taatagtatt ggtattagcg ccagctgtaa tggttgcccc acttggaaca gtccatgaat
     4201 atcccgttgc attgacaatg gcagcaactg tgtaaacatt ccctgtg
//
LOCUS       DAOORU010000178         4239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_24784_length_4239_cov_4.237333, whole genome shotgun sequence.
ACCESSION   DAOORU010000178 DAOORU010000000
VERSION     DAOORU010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24784_length_4239_cov_4.237333"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="PLF20_09320"
     CDS             <1..518
                     /locus_tag="PLF20_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81237.1"
                     /translation="HQPPAGKLEYDVILMKNNGIYHLTPKPVTIRFKGDVPPFILIPH
                     ILLMFAAMLLSLRAGLEALVKGNNTYRLALLSTILLTIGGLVFGPLVQKFAFGQFWTG
                     WPIGNDLTDNKTIAAIIFWLIATLQLKKDSSNRLWPILASIFTFFIFFIPHSLWGSQF
                     DYRTGQIKTGK"
     gene            481..1158
                     /locus_tag="PLF20_09325"
     CDS             481..1158
                     /locus_tag="PLF20_09325"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOX81238.1"
                     /translation="MIIELDKLRQVNKSSAELMQMTIIIIAITTPISIMAFYRPKFMD
                     MLKFNAFHIYEANEGWRFITYGLVHANWLHLIINMYVLYNFGLLVESYFGYFFGGKAS
                     YYYLMLYLGGLAISVVSAYQKNKGNIFYNAVGASGAISGIIFSAIIFNPFMSLRFIFI
                     PISFPAILFGAAYLIYSWFMSKKGDSQIGHDAHFWGGVYGIVFTIVLKPALAINLFEQ
                     LTRIFQN"
     gene            1213..1920
                     /locus_tag="PLF20_09330"
     CDS             1213..1920
                     /locus_tag="PLF20_09330"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HOX81239.1"
                     /translation="MKKNLSTLFLLLCILLLPILSMSQKFGLKAGYNFSSLGYDGLGK
                     DIYSNTSDIRTSTTFGGYINCKLIPLVSFQIECNYEPKGIKYGTPPLPYINASFDGIY
                     NEHFDYLCIPLLLRINLIAFQIEAGPYFGFLLKAEETKKGFQKTPGAQGEWIEEHFDE
                     VYDLKSNTKETDMGLTAGMGVSYSIGPMQILGGARYNYGFTNILKEPILNDKEEVWID
                     KKNNRNFNLYIGLGLAL"
     gene            complement(2117..3034)
                     /locus_tag="PLF20_09335"
     CDS             complement(2117..3034)
                     /locus_tag="PLF20_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81240.1"
                     /translation="MKINIKGIALFVLLAGTINLFTACSDDEEEEPLDKTPTINFIGG
                     GDYTSSDVTVPAGTVVKVGITATANTNSNAKLVKFKVTRTFNNVPETVIDSTLNNLSS
                     FTYTRNIQARTEAGTERWSFEITDKNNQSNSVALNITTQPVVNIYTAILMGGQLNVTI
                     GSFWSSVDNKVMKQAEAVLNQNKVDFLYFYGETNKATIAATDDDQAAIAWNNIFDNWT
                     VKNATRFKKVSGINWNAVTEYDDALIAENATNLVDSRANLLEVGDIVAFQTATTSANP
                     GKKGLFKVMEIAGTVGNDRTITLEVKIQK"
     gene            3147..3519
                     /gene="ssrA"
                     /locus_tag="PLF20_09340"
     tmRNA           3147..3519
                     /gene="ssrA"
                     /locus_tag="PLF20_09340"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1260 a          791 c          793 g         1395 t
ORIGIN      
        1 cccatcagcc tcctgcaggg aaactcgaat acgacgttat tctaatgaaa aacaatggca
       61 tataccattt aaccccaaag cctgttacga taagattcaa gggagatgtt cctcccttta
      121 tacttattcc tcatattctg ctgatgtttg ctgccatgct cttatcacta cgagccggcc
      181 tggaagctct ggtgaaaggg aacaatacct acagactggc tttgctatcg accatactct
      241 taacaatagg aggattagta tttggccccc tggttcaaaa atttgctttt ggtcaattct
      301 ggacaggctg gccaatcggg aatgacctca ccgacaacaa gacgattgca gcgattatat
      361 tctggcttat tgccaccctg caattaaaaa aagattcttc gaacagatta tggcccatac
      421 tcgcttcaat tttcactttc ttcatatttt ttatcccgca cagcctttgg ggttctcaat
      481 ttgattatag aactggacaa attaagacag gtaaataaat cttcggcaga acttatgcag
      541 atgaccatta tcattatagc aattaccacc ccaatttcca ttatggcctt ttatcgacca
      601 aaatttatgg acatgttgaa attcaatgca tttcatattt atgaggcaaa cgaagggtgg
      661 cgctttataa cttacggcct tgtccatgca aactggctac acctaattat aaatatgtat
      721 gttttgtata attttggatt actggttgag agctattttg gatatttttt tggaggaaag
      781 gcttcctatt attatctcat gctatatttg ggtggattag ccatatcagt tgtatcggcc
      841 tatcaaaaga ataagggaaa tattttttac aacgcagtag gtgcaagtgg tgccataagt
      901 ggaatcattt tttcggctat catctttaat ccttttatgt ctttgcgttt catatttatt
      961 ccaataagct ttccagccat attgtttggt gcagcttatt tgatttattc atggtttatg
     1021 tcgaagaaag gagatagcca gatagggcat gatgctcatt tttggggagg agtttatgga
     1081 attgtcttta cgatagtact taaaccggct ttagcaataa atcttttcga acaactgact
     1141 cgtatttttc agaattaaga gagattttcg taatcttgca aagtagccga atctatcgaa
     1201 atttattttc gtatgaaaaa aaatctttcg accttatttt tgcttttatg catattatta
     1261 ctacctattt tgagcatgtc tcaaaaattt gggctaaaag ctggatataa tttttcgagt
     1321 cttggctatg atggattggg taaggatatt tattcgaata caagtgatat aaggacttct
     1381 acgacttttg gaggatatat caactgcaaa ctcattccat tggtttcttt tcagatagaa
     1441 tgtaactacg aacctaaagg gataaagtat ggaactcctc ccctgccata tatcaatgcc
     1501 agttttgatg gaatttacaa cgaacatttt gattatcttt gcattccttt acttttacga
     1561 atcaacctta ttgcctttca aattgaagcc gggccttatt ttggcttttt attaaaggca
     1621 gaagaaacaa agaagggctt tcaaaagaca cccggtgctc aaggcgaatg gatcgaagag
     1681 catttcgacg aagtatatga tttaaagtcc aatacaaaag aaactgatat gggtctaact
     1741 gctgggatgg gggtttctta ttccattggt cctatgcaga ttttaggagg agcaagatac
     1801 aattatggtt ttactaatat actgaaagaa cccattttga acgataagga agaagtttgg
     1861 atcgacaaga agaataaccg caactttaat ctctacatag gattaggctt agctctctag
     1921 aaaaattcta actcattaga ttaaataaaa ataaatataa aggtttcccc gaagaaatct
     1981 aatatcaaaa gtttctatga ttttcccagg gtatgaattg ggataaaata atacaagttg
     2041 tttatgtggt tatagaaaag ggaggctcga tggaacctcc cctttgtttt tacagattct
     2101 aaagatgtat taaggattat ttttgaattt taacctcgag ggttatagtc cggtcgttgc
     2161 caacagtacc agcaatctcc attactttga ataatccctt tttacccgga ttggctgaag
     2221 tagtggctgt ttggaaagct acgatatcgc ccacctcaag gagatttgct cttgaatcga
     2281 caagattggt agcattttca gcaattaatg catcatcata ttcggttact gcattccagt
     2341 taataccact aaccttttta aaacgtgtgg cattttttac ggtccaatta tcgaatatat
     2401 tattccaggc aatagcagcc tgatcgtcgt cggtagcggc tatagtcgcc ttgttggttt
     2461 ctccgtaaaa gtagagaaaa tctactttat tctgatttaa aacagcttcg gcttgtttca
     2521 ttactttatt atcgactgaa ctccaaaagc ttccaatggt aacattgagt tgtccaccca
     2581 tcaaaatagc ggtataaata ttaactactg gttgtgttgt aatattcaga gctactgaat
     2641 tactctgatt gtttttatcg gtaatttcga aggaccagcg ttcggtgccg gcttcggtac
     2701 gagcctgaat atttcttgta taggtaaaac tgctcaagtt gttaagagtc gaatcgatta
     2761 cagtttcggg tacattatta aatgtacgag taaccttgaa ttttaccagc ttggcgttgc
     2821 tgttagtatt cgcggtggct gtaatcccaa ctttcacgac agtaccggct ggaacagtaa
     2881 catctgagga ggtgtaatca cccccaccaa taaaattaat ggtaggagtt ttatcgaggg
     2941 gttcttcttc ttcatcatcg gaacaggctg tgaataaatt gattgttcct gcaagtaaaa
     3001 caaatagggc aatacctttg atgtttattt tcatagcaaa tgttttttaa gttattaatg
     3061 gatgcaaaag tacaagaatc ttgccattat ttaaaaatga tattggcata attttttata
     3121 tctttgcaat tgaaacgttc attgacgggg ctgattggtt ttgacagcat gacggtggaa
     3181 ctgtaagcat gccgggcgtt agggaaaggc ccgtataaaa actaccctga tgccaataaa
     3241 tggcaacaat tataactacg caatggctgc ttaatctata cgattaagtg ctgtgctgtc
     3301 ctccaggaag ctttgccttg cagcgttctg gtaagggcat cgtaaatgcg gggctgcgtg
     3361 aggttttgtc ccgaaacctt gctaagacaa tagggactca ggaatctggc ggatgtgtcc
     3421 ctgctttgcc agagtccgaa tcatcaacag ggaataagca tgtagaaagc agttgtgctg
     3481 catgtttgga ccggagttcg actctccgca gctccacaca aaatcataaa taaaaatcgg
     3541 gcggtagata atctatcgcc cgttcttctt tccctatcct ctctcacaga cttgccttgt
     3601 ttcttcatgg aagaatgagc acactatggc tcctttatcc aaccatatag agcattcact
     3661 gctgcttatc ttcttcttta tagtttggaa aattatcttc gtcatcatca acgagaggtg
     3721 catgccgaat ttcgtttatc aataatacga aaaacagccc taccattata aagaatgcta
     3781 caatgagata ttttaacata atcgtcgaat aaattctttt aattgattta cctcctgctg
     3841 tgaacaaatc aatctaattt ctctcttttc tgtttctaaa ttgctcttca tccccttcag
     3901 atctttctta atcgcagtat atcccccctc cacattcgaa ttattaaatt ctttcaaaag
     3961 tttctcaatc tcaaggtgtc tttgcataag ataattatat ttgaaacgaa atgtgctcaa
     4021 agcttgtgcc gaatcatcaa acataaagtg gtacaatgag ttataaaata tttcgtattg
     4081 atcacctctt aaacattctc catttttaaa tagcattaaa aatagtttta ttttcaactt
     4141 attgatttat aatataatta acagtataag aaaataaaaa tccggaaacg gatacattat
     4201 ctgattccgg ataatcaaca gagataattt tctgggttc
//
LOCUS       DAOORU010000179         4210 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_24997_length_4210_cov_5.645969, whole genome shotgun sequence.
ACCESSION   DAOORU010000179 DAOORU010000000
VERSION     DAOORU010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24997_length_4210_cov_5.645969"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1533)
                     /locus_tag="PLF20_09345"
     CDS             complement(181..1533)
                     /locus_tag="PLF20_09345"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOX81241.1"
                     /translation="MKIKKLLLMSVVLLIVASSCQRNQPKQDETKMAAMKQKVDEYAI
                     VQLTTDLGQLTEKERQILPLLFQAADIMDELYWLQTYGNKEELLPGLDSLTRRFAEIN
                     YGPWDRLNNNEPFIDGVSPKPLGANFYPQDMTTEEFEKLADPSKTSLYTLIRRNAKGE
                     LIVIPYREAYSDQLTKASALLLEAASLTEDSGLKNYLQLRAKALTTDHYYESDLAWMD
                     MKNNHIDFILGPIENYEDQLFGYKTSFESFILIKDLEWTKNIEHIVTLLPKLQKSLSV
                     PEAYKAETPASSSDLGVYDAIYYAGDCNAGSKTIAINLPNDPQIQKLKGSRRLQLKNT
                     MHAKFDKILLPIATLVLDPSQLKYVRFDAFFENVMFHEIAHGLGVHQTIKGNADVRET
                     LKDTYSALEEAKADITGLHLITTMHNMGEMTDRDLMENYVTFLAENYLFASITYLSIH
                     "
     gene            complement(1754..2443)
                     /gene="trmB"
                     /locus_tag="PLF20_09350"
     CDS             complement(1754..2443)
                     /gene="trmB"
                     /locus_tag="PLF20_09350"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOX81242.1"
                     /translation="MAKKKRIFWAESKKLPHFFEPAYCELIEGFRLRGNWYPSFFSQP
                     SPIYLEVGCGKAEYTVFNARKYPLRNHVAVDIKGARMFIGARQCLQLGLTNAAFVRTQ
                     VQNLHKIFAPGEVNEIFIPFPDPQPNKPSERHRLTSPRFLDTYANILNPDHVIHLKTD
                     NLSFFEYTLNVISNEGHRLLYATYDLYSSTDDNDIKQVQTFYEKQWLASGAKILYLRF
                     QLKDVLLKPKE"
     gene            complement(2624..3739)
                     /locus_tag="PLF20_09355"
     CDS             complement(2624..3739)
                     /locus_tag="PLF20_09355"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOX81243.1"
                     /translation="MEEGELYTEITKLIVDLLPSSGCMIIPDVGGFITYNVSARIDQT
                     SNRIYPPSGRVGFNSALKVNDGLLASHYALLKGISYPQALDKIQRFTFYCISELNAGK
                     ILHFASLGNLRHDNTKNLIFEPDPHTNFLDDAFGLAPVSAMPVRRKLAEVGSMAHNRK
                     HFSRFRTHNVAEGLKWTLMILPILLLALYSVYQTGVLDGVVNYSSLGGNISQQPIDEK
                     AISNPYTIEPRIPHIEKKFQPEEYSLNRIHDAFTDTVFPVIVLNADIMRISSSKEKSQ
                     DRFDEYNAKVSVTDENSMASSQRFHLIAGCFKDINNAYRLRDELRQKGFDATLAGTSS
                     LGLTRVSIASFFSKQDAQHALNEFKTKSFQDLWILKI"
     gene            complement(3791..>4210)
                     /locus_tag="PLF20_09360"
     CDS             complement(3791..>4210)
                     /locus_tag="PLF20_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOX81244.1"
                     /translation="EQIGRVAQLLCRNEVQIASLYKKITTATDLFDPNIVKAIVSNQG
                     KALYFSRQAVPFVRNVSMEKWLELTVFYKHIGIYGYRVDTLFQIIQLPESPLEKAELL
                     EQLRWLYHGFDIYMEETHFESFSIDTPEDLSKFMNNL"
BASE COUNT         1266 a          807 c          833 g         1304 t
ORIGIN      
        1 cacgcattaa tactgcagaa gagataggca gagaatatgg agtttcacac atgactgtta
       61 atccgtattc cttaccaatt tttttagctg tattgcccga tgggtaaaaa ttacccatcg
      121 gcgatctatt ttcactatgt atttgagcct tatttgtacc ttgtgcttat tgataaacgt
      181 ttaatgtatt gataaataag taattgaggc gaataaatag ttttctgcaa gaaaagtaac
      241 gtaattttcc atcagatcgc ggtcggtcat ttctcccatg ttatgcatgg tagttatcaa
      301 atgaagaccg gtgatatcag ccttggcttc ttcaagtgca ctgtatgtgt ctttcaaggt
      361 ttccctgaca tcggcattcc cttttatggt ttggtgaacg ccgagcccat gagcaatctc
      421 gtgaaacatg acattttcga aaaaagcatc gaatctgaca tattttaatt gggaaggatc
      481 gagcaccaaa gtggcaatgg gtaataaaat tttatcgaac ttggcatgca tggtgttttt
      541 tagctgcagt cttctacttc cctttaattt ctgaatctga ggatcattag ggaggttaat
      601 ggctatggtc ttgcttcctg cattacaatc gccagcgtaa taaatggcat cgtaaacgcc
      661 cagatcggaa gatgaagcag gggtttcagc cttataggct tcaggcacgg aaagagattt
      721 ttgaagtttg ggcaataaag tcacgatatg ttcgatattc ttagtccatt ccagatcttt
      781 tatcagtatg aaggattcaa acgaagtttt atatccaaat aactgatctt catagttttc
      841 gatgggtcca agaataaaat cgatatgatt gtttttcatg tccatccacg ccaaatcact
      901 ttcataataa tgatcggtgg ttagtgcttt agctctgagt tgtaaataat tcttcaaacc
      961 agaatcttcg gttaacgaag ccgcctcaag cagtaaagcc gaggctttcg tcaattggtc
     1021 actataagcc tcacgataag gaatcacaat caattctcct ttcgcgttac gtcgaattag
     1081 cgtatataaa ctcgtttttg aaggatcagc caatttttcg aattcttctg tggtcatatc
     1141 ctggggatag aaatttgccc ctaaaggttt cggactaact ccatctatga aaggctcatt
     1201 attattaagc ctatcccatg gcccataatt gatctcggca aaacgcctgg ttaatgaatc
     1261 gagccctggc aataattctt ccttattccc ataggtttgt aaccaataaa gctcgtccat
     1321 gatatctgcg gcttgaaaaa gcaaaggaag tatctgcctt tccttttcgg tgagttgacc
     1381 taaatcagtg gttaattgaa caatagcata ttcatcaact ttttgtttca tagcagccat
     1441 cttcgtttca tcttgttttg gctgattcct ttggcagcta cttgctacaa tcagcagcac
     1501 cacgctcatc aataataact ttttgatttt catcgaattt taattttaat gggtagattt
     1561 taaaatatat atccttatcc ggaaataaaa ggttaattta ttcgtttgca atattttaaa
     1621 tagatattca taggtaatta aagcacgaat aacattaatg caccaaattt ataaatattc
     1681 acgagttgaa atgccaaaga cataaaaaca aatagcaaaa gcttgtcgga atgctcacat
     1741 catggtcgaa ggattattct ttcggcttca gcagtacatc tttgagctga aatcgaagat
     1801 aaaggatttt agcacccgag gcaagccatt gtttttcgta aaaagtttga acctgcttga
     1861 tatcattatc atctgtagat gaatagagat cgtaggtggc ataaagcagt ctatgccctt
     1921 cattggaaat cacattcaag gtatattcaa aaaaagacag gttgtcggtt ttaaggtgaa
     1981 taacatggtc agggttgagt atattagcat aagtatcgag gaatcgagga gaagtaagtc
     2041 gatggcgttc ggaaggttta ttgggttggg ggtcagggaa agggataaat atttcgttga
     2101 cttcaccggg agcaaaaatc ttatgaaggt tttgcacttg tgttcggaca aatgcggcat
     2161 tcgtcaggcc gagctgaagg cattgccggg cacctatgaa cattcgggca ccctttatat
     2221 ctacagccac atgatttctg agagggtatt tccttgcatt gaataccgta tactctgctt
     2281 tcccacagcc cacttcgagg tatataggcg atggttgcga aaaaaaacta ggataccaat
     2341 tcccacgtag tcgaaaacct tcaataagtt cacaatatgc gggctcaaaa aaatgaggaa
     2401 gtttcttgct ttcagcccaa aaaatccgtt tcttttttgc cacacttaaa aagattagag
     2461 attgcaaaat tagccgctca tccaatgcaa taaaatttca atcgtcattt gccggacttc
     2521 actcgataaa atgcttcgat aaggcgttgt ttacgttttt attatataaa aattcgattc
     2581 ttcttcaaaa agatggccat ttctctaaaa atcttcggaa gaatcaaatc ttcaaaatcc
     2641 acaaatcttg aaatgatttt gttttgaatt cgttgagtgc atgctgagcg tcctgtttcg
     2701 aaaaaaacga agcgatgctc actcttgtaa gccccaagga agaagtacct gccaaagttg
     2761 cgtcgaaccc tttttgcctg agctcgtccc tgagccggta ggcattattg atgtctttaa
     2821 aacacccggc aataagatgg aatcgctgcg aagatgccat agaattttcg tctgttaccg
     2881 atactttggc attgtattcg tcaaaacgat cctgtgattt ttccttcgaa gatgaaatac
     2941 gcattatatc agcattgagc acgattactg ggaaaacagt atctgtaaaa gcatcatgaa
     3001 ttctattgag tgaatattct tcgggttgaa acttcttttc tatatgagga attctcggtt
     3061 caatggtata tgggtttgaa attgcttttt cgtcgatggg ttgttgagaa atgtttccac
     3121 ctaacgaaga gtaattgaca acgccatcga ggacgcctgt ttgataaact gaatacagtg
     3181 ccagcagtaa tataggcaat atcataagtg tccatttcag tccttctgca acattatgtg
     3241 tccgaaatcg ggagaaatgt tttctattgt gagccatcga gccgacttct gcaagttttc
     3301 tgcgaaccgg cattgccgac acgggagcaa ggccaaaggc atcatcgaga aaattggtat
     3361 gaggatcggg ttcgaaaatt aaattcttcg tattgtcatg tcgcaaattg cccaatgaag
     3421 cgaaatgcag aatttttccc gcattcagct cagaaataca gtaaaatgta aatcgctgaa
     3481 tcttatcgag agcctgtgga taactgattc ccttaagcag tgcataatga gatgccagaa
     3541 gcccgtcatt tacctttaaa gcagaattga aaccaaccct tcccgaaggt ggataaatac
     3601 gattcgatgt ctgatcgatt cgagcagaaa cattgtaggt aataaaacca cccacatctg
     3661 gaataatcat acatccgctc gatggtaaca aatcgacaat taatttcgtt atttcagtat
     3721 aaagttctcc ttcttccata aaagaatttg aaatctttgc tcacaaattt aaaaaaactt
     3781 ctcgggagat ttacaaattg ttcataaatt ttgataagtc ttcgggagta tcaatcgaaa
     3841 aactctcgaa atgagtttcc tccatgtaaa tgtcgaaacc gtgataaagc catcgaagct
     3901 gttccagtaa ttcagccttt tctaatggcg attcgggtaa ttgaatgatt tggaatagag
     3961 tatcaactct atatccatat atgccgatat gcttgtaaaa aacagtcaat tcgagccatt
     4021 tttccattga cacatttcta acgaatggaa ccgcttggcg cgagaaatat aaagcctttc
     4081 cctgattaga aacaatggct ttgactatat ttggatcgaa taagtcggtt gccgtagtaa
     4141 tcttcttgta taaactggct atttgtactt cattgcgaca gagcagttgg gcaactcttc
     4201 caatctgttc
//
LOCUS       DAOORU010000180         4162 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_25385_length_4162_cov_4.283906, whole genome shotgun sequence.
ACCESSION   DAOORU010000180 DAOORU010000000
VERSION     DAOORU010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25385_length_4162_cov_4.283906"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2379)
                     /locus_tag="PLF20_09365"
     CDS             complement(<1..2379)
                     /locus_tag="PLF20_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HOX81245.1"
                     /translation="MYKFQICLLMIASIAILSIGCKKQLPYKDKNLTVDQRVEDLLKR
                     MTLDEKIEQLAGLPDSTGMKTVYNQRLGIPDFRMCDGPVGVRWDTSTAFPSGVSLAAT
                     FDTSLARRYGEMIARETLFKGRNYILAPCVNIHRLPTGGRNFESYGEDPFLAGKIASA
                     YIKAVQKEGVITSVKHYALNNQEWERTQVDVTADERTMREIYLPAFEMAVKAGGAYTV
                     MASYNKVNGWYASENKHLLQDILKNEWEFKGLVVSDWGATHSTTNAIRNGLDLEMPTG
                     EYLNHDKILEAIHKGEITEADIDEMVKRILWVKFQSGLFDRPQNLSYPDPSEASAELA
                     YDVAVSSIVLLKNSNHILPVDLSKVRSIAVIGPNAAVARTGGGGSSHVVPWRAIDPLT
                     QLQNVAGNQIEIHYAQGVKLGASPLQAVPSEFLITKDGKHGIIGEYFKGIELQGSPAF
                     RRIDTNIDFIWEENEPVPSLGKDHFSVRWTGYLLAPKSRNYVLYTASDDGIRLYVNNK
                     LMIDNWTDHGTTIDSARIFMKAGEKYEIRLEFFENAGSAICQLGWDYFGAEGETGEMI
                     AEAVRVAKKSDIAVVFAGSSEFIESEGYDRIGGMKLSAGQDELIDAVVKVNPQTIVVS
                     YGGTSIIMSKWGEKVPAILDVFFPGQEGSRAIIDILLGKANPSGKLPFTFIQSPQQSP
                     AYKNYQDPSKIAHYSEGIYAGYRYYEKFGIQPAFPFGFGLSYTEFEYSNLQVEPLSKD
                     SCLIRLDIKNTGEMAGTEIVQIYVVPPDASNRPVKELKGFAAVTLQPNESQNV"
     gene            complement(2411..3877)
                     /locus_tag="PLF20_09370"
     CDS             complement(2411..3877)
                     /locus_tag="PLF20_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 protein"
                     /protein_id="HOX81246.1"
                     /translation="MNRIKLIFFALIFLAVACKNEQETNLVNRSIGNYEGKKLNVYLT
                     AQNTDSRLTLSQTSVFTNQRQPLETEYVVHVDPNKSFQEFLGVGGAITDAAAETFAKL
                     TPNNQQKLIESYFNKDKGIGYSIIRTNIHSCDFSSNSYIYIEEGDSTLASFSIAHDTV
                     YRIPLIKKAINESGGNAVLFASSWSPPAFMKSNNNMLHGGKLLSKFYQAWANYYVKFI
                     KEYEKAGVPVWGITVQNEPMATQIWESCIYTAEEERDFLKNFLGPTFAKNGLTDKKII
                     VWDHNRDLMPYRASVIFSDPEAAKYAWGIGFHWYEDWSGGAQMFNNVRAVHEAWPDKN
                     ILFTEGCTESFRPDHYDYWPNAEDYGTSMIHDFNNGTVGWTDWNILLDEMGGPNHVKN
                     FCFAPVHGDTQTGELIFTPAYYYIGHFSKFIHRGAHRIMSTPSRSALLCTSFLNPDGS
                     IVTIVMNKSDQNIPYLLIVADGLAQLSIPAHAIQTIVY"
BASE COUNT         1172 a          895 c          835 g         1260 t
ORIGIN      
        1 tacgttttgg ctttcgttgg gttgaagcgt aacagccgca aatcctttaa gctctttcac
       61 tggtctgttg ctggcatcgg gaggaacaac ataaatctga acgatttcag ttcctgccat
      121 ctctccagta ttttttatat cgagacggat aaggcacgaa tctttggata ggggttccac
      181 ctgcaagttg ctgtattcga attcggtata ggataatccg aatccgaaag gaaatgcagg
      241 ctggattcca aatttctcat aatatctgta acccgcataa atgccttcac tgtaatgagc
      301 aatcttagaa ggatcctgat agtttttata agcaggcgat tgctgaggcg actgaatgaa
      361 agtgaaaggt aattttcccg aaggattcgc tttacccaac aatatatcaa taattgctct
      421 cgaaccttct tgcccaggga agaatacatc caagatggct ggaacttttt ctccccattt
      481 actcatgatt atgctcgttc ctccgtatga aaccactatg gtttgtggat taaccttcac
      541 cacagcatcg atgagttcat cctggccggc ggatagcttc atacctccaa tcctgtcgta
      601 tccttcactt tcaataaatt cgctcgaacc agcgaaaaca acggctatat cactcttttt
      661 agcaacccga actgcctcgg ctatcatttc acccgtttcg ccctcagcac caaagtaatc
      721 ccaacccaac tgacatattg cagagccagc gttttcaaaa aattcaagcc tgatctcata
      781 tttttcacca gctttcataa aaatccgagc cgagtcgatg gttgtaccat ggtcagtcca
      841 attgtctatc atcaacttgt tatttacata aagcctgatg ccatcgtcgc tggcagtata
      901 taaaacgtaa ttccggcttt tgggagccaa aagatacccc gtccatcgta ccgaaaaatg
      961 gtctttgccc aatgaaggaa ctggctcgtt ctcttcccaa ataaaatcaa tattggtatc
     1021 tatacgtcga aatgcaggag aaccttgcag ttcgattcct ttgaagtatt ctccaataat
     1081 accgtgttta ccatcctttg ttataagaaa ttctgaaggg actgcctgca aaggacttgc
     1141 accgagtttt actccctgtg cgtagtgaat ttctatctgg ttgccggcaa cattttggag
     1201 ttgagttaga ggatcgatgg ctcgccaagg aacgacatgc gagctcccgc ctccacctgt
     1261 ccttgcaaca gcagcattag gtccaattac cgctatggac ctcactttgg agagatcgac
     1321 aggtagaata tgattggaat ttttcagcaa aacaatggaa cttacggcaa catcataagc
     1381 cagttctgcc gatgcctcgg aagggtcggg ataagaaaga ttctgcgggc gatcaaaaag
     1441 acctgattga aatttaaccc aaagtattct tttcaccatt tcatcgatat cagcctcagt
     1501 gatttctccc ttgtggatgg cctctaagat tttatcatgg ttaagatatt cgcccgtggg
     1561 catttccaaa tccagaccat tgcggatagc attggtggta gaatgtgttg caccccagtc
     1621 ggataccacc agacccttga attcccattc attttttaga atatcttgta acaaatgctt
     1681 gttttctgaa gcataccagc catttacctt attataggat gccataaccg tataagcacc
     1741 acctgctttt acagccattt cgaaggctgg caggtatatt tccctcatcg ttcgttcatc
     1801 ggctgtcacg tcaacttgag tgcgttccca ttcctgattg ttgagggcat aatgttttac
     1861 cgaagtgatt acgccttctt tttgtacggc ttttatgtaa gctgaagcaa ttttaccagc
     1921 cagaaatggg tcttcaccat aactttcgaa atttcttcct ccagtaggca aacgatgaat
     1981 attgacgcaa ggagccaaaa tgtaatttct tcccttaaac aatgtttctc tggctatcat
     2041 ttcgccatat cgccttgcca gagatgtatc gaaggttgct gcaagtgaaa ctcccgaagg
     2101 gaaagccgtc gaagtatccc acctcacgcc aaccgggcca tcacacatcc tgaagtcggg
     2161 tatgcccaaa cgttgattat aaactgtttt catgcctgta gagtcgggaa gaccagccaa
     2221 ttgttcaatt ttctcatcca aagtcattcg tttgagcaaa tcttcaactc tctgatccac
     2281 agtaagattc ttgtctttat aaggaagctg ttttttgcat cctattgaca atatagctat
     2341 ggatgcaatc attaataaac aaatctgaaa tttatacatt gtgtaaaaga tgattttttt
     2401 gcagaaagta tcaataaaca atggtttgta tagcatgagc cggaatactc agttgagcca
     2461 aaccatcggc aacgatgaga aggtaaggaa tattctggtc ggatttgttc ataacgatgg
     2521 ttacaatgct tccatccggg tttagaaatg aagtgcataa tagggcgctg cggcttggtg
     2581 tgctcattat gcgatgcgca cctctatgaa taaatttgga aaaatggcca atataataat
     2641 aggcaggggt gaatattaat tcgccggttt gtgtgtctcc atgtacagga gcaaagcaga
     2701 aattctttac atggtttggt ccgcccattt catcgagcaa gatattccag tcggtccaac
     2761 caacagtacc attattgaaa tcatgaatca tggaagtgcc ataatcctcg gcattaggcc
     2821 agtagtcata atggtcgggt ctaaaacttt cggtacatcc ttcggtgaag aggatattct
     2881 tatcgggcca agcttcgtgt acagctctaa cattattgaa catttgtgct ccccccgacc
     2941 agtcttcata ccagtggaag ccgatacccc aggcatattt tgctgcttcg ggatcgctaa
     3001 agatgactga ggctcggtaa ggcattagat cacggttatg atcccaaacg atgattttct
     3061 tgtcggttaa accatttttc gcaaacgtag gtccgagaaa attcttcaga aaatcacgct
     3121 cttcttctgc cgtatagata catgattccc atatttgagt agccatgggc tcattctgaa
     3181 ccgtgatgcc ccaaacaggt acacctgcct tttcatattc cttgatgaat ttcacgtaat
     3241 agttagccca tgcttgataa aattttgaga gaagtttccc tccatgcaac atgttattgt
     3301 tacttttcat aaatgcaggc gggctccatg aactggcaaa taaaactgca tttccaccac
     3361 tttcgtttat ggcttttttg atgagcggaa tacgatagac cgtatcatgg gctatgctga
     3421 aggatgcaag agtagaatcg ccttcttcaa tgtatatata actattacta ctgaaatcgc
     3481 aactgtgtat attggttcta atgatagaat aaccaatgcc tttatctttg ttgaagtaag
     3541 attcaattag tttttgctga ttatttgggg tcaatttggc aaaggtttcg gctgcagcat
     3601 cggtaattgc tccacccact cctaaaaact cctgaaaact tttgtttgga tcaacgtgaa
     3661 ccacgtattc cgtttcgagg ggttgcctct ggttagtaaa tactgaagtt tgggaaagag
     3721 tcaaacgcga atctgtgttt tgggcagtta gataaacatt cagttttttg ccttcgtaat
     3781 tccctattga tcggtttaca aggtttgttt cctgctcgtt tttacatgct actgccaaaa
     3841 atatcagggc aaaaaagatt aatttgattc ggttcataag gttaaaaaat ttgattatgc
     3901 ttcgatgatt gataaaaata tttacaggta aagatagaaa attatcggac aatgaataag
     3961 gtccataaaa atatcgaaaa ctgaaaccat tattcccata tattaccagt gaatttgttc
     4021 tcaataaaaa caaaaagagg ccacactttt ggtaagagcc tcttttcaat atcaatagcg
     4081 aacagcttat tttaagagtt tcttagtaaa agtggttttg taagttaagg ggatggtcat
     4141 gttttgctgg ccgctgagta aa
//
LOCUS       DAOORU010000181         4153 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_25462_length_4153_cov_5.335041, whole genome shotgun sequence.
ACCESSION   DAOORU010000181 DAOORU010000000
VERSION     DAOORU010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25462_length_4153_cov_5.335041"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(567..1040)
                     /locus_tag="PLF20_09375"
     CDS             complement(567..1040)
                     /locus_tag="PLF20_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81247.1"
                     /translation="MNLLSGCDRFSCFTRLANLTLVFCLIVSFWACTDDNDISPIPDV
                     YVNIVINPASIEYGNLNIPGNYALIKGGYRGIIVYHVIDNQYMAYERTCTYDWEKPCS
                     KLIIDASGLIARDTCCKSSFLLIDGSPMEGSLATIPLKQYKTMYDPNFGRLYITN"
     gene            1085..1399
                     /locus_tag="PLF20_09380"
     CDS             1085..1399
                     /locus_tag="PLF20_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81248.1"
                     /translation="MRGMRFRRIIIMMFLVMLVDISCSTRESQTAKQQKEIEMRKEER
                     RKKEEAAYQSKIERHEKIQSEEGRKLIQQANEHRNKLDKQNNKKKFSLKNLFGKDEPA
                     CH"
     gene            1492..1950
                     /locus_tag="PLF20_09385"
     CDS             1492..1950
                     /locus_tag="PLF20_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81249.1"
                     /translation="MEDLIVLLIFIAWIGYTFYKESKKHKKTVGKPPTLPVDIPEQAD
                     KRRINPQMEEFDYDTDFEGGIPVTEAKENVIMASPEVSRAETSSPIQFIPLINQIHRP
                     DFESIEYVRDEQTKIHELMKEQPEDEASKEFSFDLRQAVIYSCILHRPYD"
     gene            2148..>4153
                     /locus_tag="PLF20_09390"
     CDS             2148..>4153
                     /locus_tag="PLF20_09390"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HOX81250.1"
                     /translation="MIRKLLFTLGIILATHVLVFSQATGTLRGRIIDKETKEPIPFAN
                     VIIEMNGVQAGGATSDFEGNYVIKPINPGKYDIRCSYVGYKTLLTKGIIIGAGKIEFY
                     NIELEPTTVALSEIEVTSYKVPLIEKDQTTVGATVTAEQIDKMPNRSATAVAITVGGV
                     FSSDGERGSVRGARSEATVTYIDGVKVTGSASLPPSAIEQVSVIISGLPAQYGDATSG
                     VINITTKGPSNIYAGGFEAQTSELLDNFGYNRFGFNLQGPLIRKKNKETGESRSILGF
                     FFAGDVILQGDAAPSPIGRWKVKDDVLQYLKENPLRPTGLGYGTYPNAAFLASDDLEY
                     VKRSQNADAFNINLSGKIDLRLSSTTNFTLGGSTVYYKSNAFGYSRSLMNWENNPFHD
                     ELTYRGYARFTQRFPAAADSRSLIKNVYYSLQADYTKYHSRTQSAQHKDNLFKYGYLG
                     RFDTYTVRSYELGSDTINGQVYNNVYIHNGYRDTMVTFQPSDFNRDLARYTETYYNFY
                     PDVTGNWENFDQILLGGGYINGMSPSAIYSLWQAPGTVYNGYSEYDADQIGFNGMLSA
                     DIGNHEVQIGFQMEQRSESYYGYAPTYFWQLMRGLTNFHIRELDKSNPVLVSRDGVFQ
                     DTIYYYRKYDANSQRTFDRNLRKKLGLPVDGLDFINIDSYDFAS"
BASE COUNT         1342 a          793 c          840 g         1178 t
ORIGIN      
        1 ttcgagtatc ggtcgggaaa aatggtaacg gaagttgcat atcaaaaaat ttttgtccga
       61 aaatacgaca aattgaaata cggtggtact tttgtaaaat attgatatgc aattatagga
      121 tcaggagttc tgaatttaca aaatcttacg aattgcaggt tttcaatgct tttggttctt
      181 gcatttataa tagaaagata tcacgtaacg attatcagtt aaatctttct aatcagagct
      241 cggatattta tttttatcgg gcttacgatg aatcaggaac tttgggaact ggtaaactga
      301 tgattaggaa ataaggaagg actgcattgt aattgttgaa tctctaacga gactaataat
      361 ttgtcaatgc tcaaatgact ttagtctttt ttgggctaat tatacgtgat gcgagagaaa
      421 agttcccgca aaatgacaca atttctgaac tttcaagact tttactcacc atcccttatc
      481 cacctatacc tgtttgccat cttctatccc gtacccgtca taatcctatt catgagcaga
      541 gtgtatataa aaacatacag tatgacttaa ttcgtaatat acaatctgcc aaaattcggg
      601 tcatacatcg ttttgtattg ttttaatgga atagtggcta aacttccttc catgggcgaa
      661 ccatctataa gtagaaaact cgatttgcag catgtatcgc gggcaatcaa tcccgaagca
      721 tctattatta atttcgaaca gggtttttcc caatcgtaag tgcaggttct ttcgtatgcc
      781 atgtattgat tgtcgattac atgataaacg ataattccac ggtatccacc tttaatcaat
      841 gcataattgc caggtatatt caaattgcca tactctatcg aagcaggatt gattacaatg
      901 ttcacataca catcgggaat gggcgagatg tcattatcgt cggtacaagc ccaaaaggaa
      961 acaatcaagc aaaatacaag ggttaggttt gctaacctgg tgaagcatga aaatctgtcg
     1021 caaccgctta ataaattcat attgatttat tttgtttaag tttgcaaaga taatgccctt
     1081 ttatatgcga ggaatgagat ttcggcggat aatcataatg atgtttctgg tgatgctcgt
     1141 agatatttct tgtagcaccc gagagagtca gaccgcaaaa cagcaaaagg aaatcgaaat
     1201 gcgaaaggaa gaaagacgaa agaaggaaga ggcagcttat cagagtaaaa ttgaaagaca
     1261 tgaaaaaatt cagagcgaag aagggcggaa attgattcag caagccaatg aacatagaaa
     1321 caagctggac aagcaaaata acaaaaagaa attttcgttg aaaaatctgt ttggcaaaga
     1381 tgaaccggct tgccattaac atacatcaca cgaaaagttt atttggtttt acttctttct
     1441 gtgaggagag gatatcaaga atttatttaa ttttaccatc aaatttgcta tatggaagat
     1501 ctgatcgttt tgctcatatt tattgcatgg ataggctata cattctataa ggaatcgaag
     1561 aaacacaaga aaacggtggg gaagcctccg accttgcctg ttgatatacc cgaacaagca
     1621 gataaaagaa gaatcaaccc ccagatggaa gaattcgact atgatacaga cttcgaagga
     1681 ggaattccgg ttacagaagc aaaggagaat gtgataatgg catcccccga agtttcaagg
     1741 gccgaaacca gttcacccat ccaatttata cccctgataa atcaaattca tcggcctgat
     1801 tttgagagca ttgaatatgt acgtgatgag caaaccaaaa ttcatgagtt aatgaaggaa
     1861 caacccgagg acgaagcctc taaggagttt tctttcgatt taaggcaagc agtgatttat
     1921 tcttgtattc ttcatcggcc ttatgactga aaatagaaat ataggggctg acaaaatttt
     1981 tactgagtat agaaaagtct ataattaatt cataatcaat aaataacaac ctcaaaatat
     2041 tctcgaaaat ttcaaatata aaacaaaggt ttgtaaaata aacttaaatc tttctaattt
     2101 taccgacttt aaatgcacaa cgcaaatcat caataaatct atctggtatg ataagaaaac
     2161 tactatttac actgggtatt atactggcaa cccacgtgct ggtattttcc caggcaacgg
     2221 gtaccctgag ggggagaata attgacaagg agacgaaaga acccattcca tttgccaatg
     2281 tcatcatcga aatgaatggc gtacaggctg gtggtgcgac ttctgatttc gaaggaaatt
     2341 atgtcatcaa gcccattaat cccgggaaat atgatataag gtgttcttac gttggttaca
     2401 aaacccttct aactaaaggc attatcatag gtgcggggaa aatcgaattc tataacatcg
     2461 aattggagcc caccactgta gctttaagtg aaattgaggt taccagctat aaagtacctc
     2521 tgatagagaa ggaccaaacc actgtgggag ctacggtaac tgcggaacag atcgataaga
     2581 tgcctaatcg ttccgctaca gctgtggcaa taaccgtagg cggtgtgttt tcatctgacg
     2641 gtgagcgagg cagtgttaga ggtgcacgaa gcgaagccac tgtaacttac atcgacggag
     2701 taaaagttac cggatcggcc agcctgcctc cttcagctat cgaacaggtt tcggtaatta
     2761 tttcgggatt accggcacag tatggcgacg ccacctccgg cgttatcaat atcaccacca
     2821 aaggtccatc caacatttat gctggaggtt tcgaggctca gacttccgaa ctcctcgaca
     2881 actttggata taaccgattt gggttcaact tgcaaggccc tctcatccgt aagaaaaata
     2941 aggaaactgg cgaatcgaga agcatcttgg gatttttctt tgctggcgat gtgattttac
     3001 agggagatgc tgcccccagc cctattggaa gatggaaagt caaagacgat gtattacaat
     3061 accttaagga aaatcctctt cgacccacgg gtttaggcta tggaacttac cccaatgccg
     3121 cattcctcgc ttccgatgac ctcgaatatg tgaaaagatc tcaaaatgcc gacgctttta
     3181 atattaacct ttcgggaaaa atagatttgc gcttatcctc aactaccaat tttacgttgg
     3241 gtggttcgac agtgtactat aaaagcaatg cctttggata ctcccgttcg ttgatgaact
     3301 gggaaaataa tcctttccat gatgaactga cttatcgtgg ttatgccagg tttacgcaga
     3361 gattcccagc cgcagccgat agcagatctc tcataaaaaa cgtttattat agccttcagg
     3421 cagactacac aaaataccat tcaagaactc aaagcgccca gcataaagat aatctattca
     3481 aatatggata tttgggacga ttcgatacct ataccgtacg ttcttacgaa ttgggaagcg
     3541 atactattaa tgggcaagtt tataataatg tatatataca taatggctac agagatacca
     3601 tggtaacctt ccaaccctcc gattttaaca gagatctggc acgctatact gaaacctact
     3661 ataattttta cccggatgtt accggcaatt gggaaaattt cgaccaaatt cttctgggag
     3721 gtggttatat caatggaatg agtcccagtg ccatttattc tttgtggcaa gcccccggaa
     3781 ccgtttacaa tggatatagt gaatatgatg ccgatcagat tggattcaat ggtatgttat
     3841 cggccgatat tggtaatcat gaagttcaga taggattcca aatggaacaa agaagtgaaa
     3901 gctattatgg gtatgcccct acctatttct ggcaattaat gagaggactt actaacttcc
     3961 atattcgtga attggataaa tctaacccgg ttcttgtttc ccgcgatggg gtctttcagg
     4021 atactatcta ttattacaga aaatatgacg caaattcaca acggactttc gaccgcaatc
     4081 tgcgtaaaaa attaggtctt ccagtagatg gccttgattt tatcaatatt gattcgtatg
     4141 attttgcttc gaa
//
LOCUS       DAOORU010000182         4084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_26047_length_4084_cov_4.144949, whole genome shotgun sequence.
ACCESSION   DAOORU010000182 DAOORU010000000
VERSION     DAOORU010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26047_length_4084_cov_4.144949"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(402..827)
                     /locus_tag="PLF20_09395"
     CDS             complement(402..827)
                     /locus_tag="PLF20_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81251.1"
                     /translation="MKNSFGWLIAGFLFFELLMIQSCSKENNSSQYAYDLQPHIENLY
                     SSFIYRYNTGYYTADTGYKLLLNPENKEFEIHLTVQDYKSGWMKLSFFDVDQEIVSTD
                     TLRFNYDSILQIPVSIDSLSLKSQIFTGIIYFSLQPSFK"
     gene            complement(989..1480)
                     /gene="pyrI"
                     /locus_tag="PLF20_09400"
     CDS             complement(989..1480)
                     /gene="pyrI"
                     /locus_tag="PLF20_09400"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005829083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HOX81252.1"
                     /translation="MIFIFILTHMKPESRKKLIVSAIQNGTVIDHIPSHAVIQVMRIL
                     NLIQTDHQVLFGMNLDSKKYGNKGIIKIRDKFFDDVEINKIALVAPTATLIEIRDFEV
                     ISKKQAEIPDIVEGIVKCFNPKCITNYEQVRTRFRVIDKQNLRLLCHYCEKITKNENI
                     EFI"
     gene            complement(1496..2404)
                     /gene="pyrB"
                     /locus_tag="PLF20_09405"
     CDS             complement(1496..2404)
                     /gene="pyrB"
                     /locus_tag="PLF20_09405"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HOX81253.1"
                     /translation="MKNKSLISINDFCKEEYLQILDLATYFEKNPNQKILEGFVVATL
                     FFEPSTRTRLSFESAVNRLGGRVIGFSDAALSSTTKGESLADTIRTVSNYADLIVMRH
                     PIEGSARWASQVASVPIINAGDGSNQHPTQCLLDLFTIRQTQGTLDNLDIAFVGDLKY
                     GRTIHSNVIALSHFNTTFHLVSPEELKLPSAVKMHIKSNNLQYFQYTDLEEVIPKVDI
                     IYMTRIQRERFADPIEYERVKNSYTLTKKMLEGSRENLKILHPLPRVNEISVDVDLDP
                     RAYYFKQALNGVYIRQALIASILGLA"
     gene            complement(2518..3882)
                     /locus_tag="PLF20_09410"
     CDS             complement(2518..3882)
                     /locus_tag="PLF20_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOX81254.1"
                     /translation="MRRNVIIIGAAGRDFHNFNTFFRGKSSYNVVAFTAAQIPDIDGR
                     KYPPQLAGEDLYPYGIPIMAEQNLPQLIKDLDVHDCVFSYSDIPYQRVMGLSAIVNAA
                     GANFMLLGPRETMLKSTKPVIAVGATRTGCGKSQTSRRIIEILMKHGLKVVAVRHPMP
                     YGDLVAQKMQRFATIEDLHRHKCTIEEMEEYEPHLVRGNVIYAGVDYEAILRAAENDP
                     NGCDVILWDGGNNDFPFYKPDLMIAVADPHRPGHEVSYYPGEVVARIADIIVINKIDT
                     ACNENILTVRQNIARINPNAIVVDGASPLTVDHPELIRGRKALVVEDGPTLTHGEMKI
                     GAGTVAALKYGASELIDPRPYAIGKIADTFHIYPNIAALLPAMGYGDQQIKDLEITIE
                     NTPADVVIIGTPIDLTRIIKINKPTVKIGYDLQEIGHPDLEELLVNFLEKHNLTKKAP
                     KSCC"
BASE COUNT         1272 a          751 c          860 g         1201 t
ORIGIN      
        1 cgtgtacaag aactttcatc ttgcaagtag taatgtatgc ccgtcataaa aaaaaccggc
       61 gcgtatccct acgcaccgga aaaaagtaat caaaattact taaacaacaa aaacaattag
      121 taggtaaaga gagtttagtt tggtttggta ttaccaggta ttaacctgca acagctctaa
      181 aaaaagtaca actatcatgc cataataata aatatcctta aaatcaatga gaaaatgaca
      241 tgtgagggct accctttggg aatagaatat tccgaaaaca acaatttatt tcttaaaaag
      301 agacttgata ctcatttaac atattgatta ataatcaatt tacttaatat tttaaatttc
      361 atccgaaatc ggagaattat ccatattcga cgaagatttc attatttgaa tgaaggttga
      421 agagaaaaat agataattcc ggtaaaaatt tgtgatttca aactaagaga atctatcgaa
      481 accggaattt gcaatatcga gtcataattg aaacgcaagg tgtcagtaga gacaatctct
      541 tgatctacat caaagaaaga taatttcatc caccctgatt tatagtcctg aactgtaaga
      601 tgaatctcga attctttgtt ttcgggattc aataacagtt tgtaacctgt atcggcagtg
      661 taatatccag tattatacct gtaaatgaag gaagaatata gattttcgat atgtggttga
      721 agatcataag catattggga agagttattt tctttcgaac aggattgaat catcaataat
      781 tcgaagaaaa ggaacccagc aatcaaccac ccgaatgaat ttttcatcaa tatagccaat
      841 ttgggataca aaattatgca aaacatggca acctcccaaa gaagggaagg aagatcaggt
      901 agcgacgcag gcattcatgg ttaacatgat cctccttcct ttgtttggga tctgcaataa
      961 gatgagcaaa gaagaaggca agcagaggtc agatgaactc gatattttca tttttggtaa
     1021 tcttttcgca gtaatggcag agcaaacgca aattctgctt atcgatcacc ctaaagcgtg
     1081 ttcgtacttg ttcgtaattg gtaatgcatt tcggattgaa acatttgacg ataccttcga
     1141 caatatcggg tatttcggct tgtttcttcg atatcacttc aaaatcacga atttcgataa
     1201 gggtagcagt aggggctaca agggctatct tattgatttc cacatcatcg aaaaatttat
     1261 ctctaatttt gataattccc ttattgccat actttttact atcgaggttc attccaaaga
     1321 gcacctgatg gtcggtttga atgaggttga ggatacgcat tacctgaata acagcatggg
     1381 agggaatatg gtcgattact gttccatttt gaatggcgga cacgatgagt ttttttcggc
     1441 tttcgggttt catatgggtt agaataaaga taaaaatcat gattacattt tagaatcaag
     1501 caaggccaag aatagaagcg atcaaagctt ggcgtatata aacaccattg agggcctgtt
     1561 taaaataata ggcacgggga tcgagatcca catctacact aatctcgttg actctgggca
     1621 aagggtgtag aattttcagg ttttcccttg aaccttcgag cattttttta gtaagagtat
     1681 aagaattttt tactctttcg tattcgatgg ggtcggcgaa gcgctcgcgc tgtatacggg
     1741 tcatgtaaat aatatccacc ttaggaatta cttcttcgag atcagtatat tgaaaatact
     1801 ggaggttgtt agatttaatg tgcattttga ccgcacttgg caatttgagt tcttcgggag
     1861 agacgaggtg aaaggtggta ttaaaatgag atagtgcaat gacgttggaa tggatagtac
     1921 gaccatattt caggtcaccc acgaaggcga tatctagatt gtcgagagtg ccttgtgtct
     1981 ggcgaatagt gaaaagatcg agaaggcatt gtgttggatg ctgattagaa ccatctccag
     2041 cattgatgat ggggacactt gctacttggc tagcccatcg tgcagaacct tcaataggat
     2101 ggcgcattac aatcaggtca gcataattac taaccgtgcg tatggtatcg gccaaacttt
     2161 cacccttcgt ggtgctgctc aaggctgcat cgctaaaacc aatcacacgg cctcctaaac
     2221 ggtttactgc actttcgaaa ctcaggcggg tacgggtact tggttcaaaa aacaaggttg
     2281 ctacaacaaa accctcaaga atcttttgat taggattttt ctcgaaataa gtggcaagat
     2341 caagaatttg aaggtattct tccttacaga agtcattgat cgaaatcaga cttttattct
     2401 tcatgttcat agtgggtggt tattttttcc gtttcaaatt taagttcaaa gatcaaaaaa
     2461 agtaaaaatc tttctggttt ctaaaaaaaa accccgcaat tgcggggtct tcgaatatca
     2521 acagcagctt ttgggagctt ttttggtgag gttatgtttt tcgagaaaat tcacgaggag
     2581 ttcttccaga tccggatgtc caatttcttg aaggtcgtaa ccaattttaa cagtaggttt
     2641 attgattttt attatccggg tgaggtcgat gggagtacca attataacaa catcggcagg
     2701 tgtattttca atggttattt cgagatcttt aatttgttga tccccatatc ccatagctgg
     2761 gagcaaggca gcgatatttg gataaatgtg gaaagtatcg gcaattttac caatggcata
     2821 agggcgtggg tcgataagtt cggaggcgcc atatttcaaa gcggcaaccg taccagctcc
     2881 aattttcatt tcaccgtggg ttagtgttgg gccatcttcg actaccagag cttttctgcc
     2941 cctgatgagt tcagggtggt caacggtaag cggagatgca ccatccacaa caatagcatt
     3001 aggattgatg cgtgcaatat tttgacgaac ggtgagaata ttttcgttgc aagccgtatc
     3061 aatcttgttg ataacgatga tatcggctat acgggcaaca acttcaccgg gataataaga
     3121 aacttcgtgg cctggccggt gtgggtcggc tacagcaatc ataaggtctg gcttgtaaaa
     3181 agggaaatcg ttgtttccac cgtcccaaag aataacatcg cagccattgg ggtcattttc
     3241 ggcagctcta agaattgctt cataatcgac cccagcatat attacatttc cacgaactaa
     3301 atgaggttca tattcttcca tttcctcaat agtgcattta tggcgatgaa ggtcttcgat
     3361 ggtagcaaaa cgttgcattt tttgtgccac aaggtcgcca taaggcatgg gatggcgcac
     3421 agccacgacc tttaagccgt gtttcattaa aatttcgatg atgcgacgag aggtctggct
     3481 tttcccacaa cccgttcgcg ttgcgcctac ggcaattaca ggctttgtac tctttagcat
     3541 ggtttcacga ggacctaaaa gcatgaaatt agccccagca gcattaacaa tagccgaaag
     3601 gcccataact cgctggtaag gaatgtcact ataagaaaat acgcaatcat gcacgtcaag
     3661 gtctttgatt aattgcggca agttttgttc ggccataata gggattccat agggataaag
     3721 atcttctccg gctagttggg gaggatattt tcgaccatcg atgtcaggaa tctgagcggc
     3781 tgtaaatgcc actacattgt aagaactctt accgcggaag aaagtattga agttgtgaaa
     3841 atcacgaccc gcagcaccaa taataataac atttcgtctc ataaaatatt tgatttgggt
     3901 ttgtttattc tcaatcgtgc aaaattagag ctttagtcat tgcaagccaa ataatgaatc
     3961 attggaaatc tataaggata taaatcatta tcaatcaaga aacaaaacat ctgataaatg
     4021 tacaacaaga catgtaactg tttatatatc aaaacaataa taaaaacatg agtccgactt
     4081 cttg
//
LOCUS       DAOORU010000183         4072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_26154_length_4072_cov_4.525765, whole genome shotgun sequence.
ACCESSION   DAOORU010000183 DAOORU010000000
VERSION     DAOORU010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26154_length_4072_cov_4.525765"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLF20_09415"
     CDS             <1..371
                     /locus_tag="PLF20_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81255.1"
                     /translation="TLKLILSDTLTQITDEPLNLETNQMVQFSLDAKTYPAKSEAFIV
                     VSLSHPLQEDAKIVVNYGHGKTKTGGVVIKLLKNPVTNTFVAPLNSTTGWNAATTTWL
                     SLVPEQANLSIQKILITALK"
     gene            662..3925
                     /locus_tag="PLF20_09420"
     CDS             662..3925
                     /locus_tag="PLF20_09420"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX81256.1"
                     /translation="MNQIFRKLCMIVLIMIGAGSSFAQITTSGINGKVTGTNGESLPG
                     ATVILIHVPSGTQYGTTTDINGFYRIPNVNVGGPYTLKISYVGYEDFLKENIFLTLGQ
                     YSRINVSLAEKLSQISGVEITAERSAIFDLNRTGSETNITRDKIDKLPTIGRNLTDYT
                     RLTPQARVQETSDGPNLTVAGMNNRYNAIFIDGAVNNDVFGLAGSGTNGGQTGISPFS
                     MDAIDQLNIAIAPYDIKLGGFAGAGINAVTRRGTNEIEGSAYYYFRNQDLAGKTPTDD
                     SNIERKKLPDFSSKTYGVRLGGPIIKDKVFFFGNVEIQKDETPQPFEFSTYEGNSDVD
                     KISQLIEKVKSYGYDPGGFQGVIKKLDGTKIFGRVDWNINQNHKLTLRHQYTKGTKTE
                     PGYQTNTNITFENAGIYFPSTTNSSALELKSIFGNKFANNLIVGLTFVRDDRDPMGQN
                     FPYVSIRDGKGSINLGSEQFSTANQLDQDIITLTDNFEWYSGKHTLTFGTHNEFYNMY
                     NLFIRQNFGVYTYNSIDDFLNNDTATQYDRTFSMTDGKTGDGSSAATKFKALQLGFYA
                     QDEYQALDNLKLTFGLRVDIPMFLDDPIVNEDFNNNIIPVLEQNGWDLQGAQTGQMPD
                     VHLLWSPRFGFNWDVFNDQSTQVRGGIGIFTSRIPYVWPGGVYNNNGLMLGGMRVTYV
                     SNPTSPYLIFNPDWNNQPGWKPGDPVTPSGQIDLFAKNFKFPQVWRMSLAVDQKLPWD
                     VIATGDVTYTKNINNVLYKNLAYKKSGLHLTGTGDNRPLWEKIDLQGAKYTDIMWATN
                     TSLGYTYNLTAQLQKTFHNGLYTSLAYTFGAARSMNDGQSSQNSSQWRVPNVRGKNDL
                     DFSISDFDMGHRIVGYISWKIDYLKHCATTIGFFYSGQSGQRFSWGYQDNTKSWLGED
                     NQSLELMYVPKDQNDIILLDVKDANGNIVISKEQQWNDLTAFIESDDYLRSRRGMYAE
                     RNAARTPFEHNIDLHLAQDFYVNVAGKRNTLQITFDIFNLGNFINSDWGRKYYVSGYY
                     GNYPLLNFQGLKADADGNKTVPTFTFQKPKGEVWNIDDSGLISSRWMGQVGIRYIFN"
BASE COUNT         1285 a          857 c          772 g         1158 t
ORIGIN      
        1 gaactctgaa actgatactc tcagatactc tgacgcaaat tacagacgaa ccattaaatc
       61 tcgaaaccaa tcaaatggtt caattttcac ttgatgcaaa aacatatcct gccaaaagcg
      121 aagcttttat agtagtcagc ctctcccatc ctttacaaga agacgccaaa atcgtcgtaa
      181 actatggcca cggaaaaacc aaaacaggag gcgtggttat taaattatta aaaaaccctg
      241 ttaccaatac ctttgttgca ccattgaact caactacggg atggaatgca gccactacca
      301 catggttgag cctggtaccc gaacaagcca acctctccat tcaaaaaatc ctcattactg
      361 ccctaaaata aaccttcaaa gaatgattct ccttatcact cgaaaagtca tatgggtaat
      421 gtatctctga atcattttat aaccacctga tcaataaaat attttatctg cttttaaaga
      481 gaattaataa aaactttaag tttatttaaa tgatattctc atactcttcg atagttttgc
      541 aaggatttcg aagaggctgt ttacatttat tgataacctt ttgataaaat gaatacacat
      601 atatatttat ctaagttatg atttcggaca actcaacctc aataatctaa cccattaaat
      661 tatgaatcaa attttccgaa aactctgcat gattgtactt atcatgatag gagcaggcag
      721 ttcgtttgca cagattacta cttctgggat caatgggaaa gttaccggga ctaatggtga
      781 aagtttaccc ggagcgacgg tcatacttat ccatgttcct tcaggcacgc agtatggtac
      841 aaccaccgat atcaatggtt tctatcgaat tcccaatgtc aacgttggtg gtccttacac
      901 cttgaaaatt tcgtatgttg gttacgaaga ttttctgaaa gagaacattt tccttacatt
      961 aggacaatat tcgcgaatta atgtctcttt agccgagaag ctttctcaaa tttccggtgt
     1021 cgaaatcact gctgaaagaa gtgcaatctt cgatttaaat cgaaccggta gtgaaaccaa
     1081 tataacacgt gacaaaatcg ataaattacc aaccatcggg cgcaatctaa ccgactacac
     1141 tcgattgacg cctcaagccc gggttcagga aaccagcgat ggacccaatc ttacggtagc
     1201 tggcatgaac aaccgctaca atgccatatt tatagatggt gccgttaaca acgatgtatt
     1261 cggattagcc ggaagtggaa ccaacggtgg acaaaccggt ataagcccct ttagtatgga
     1321 tgccatcgat cagttgaata ttgctattgc tccttacgac atcaaattgg gtggttttgc
     1381 cggtgcagga atcaatgccg taactcgaag aggaactaat gaaattgaag gttcggccta
     1441 ttattatttc cgtaatcaag acctcgcagg aaaaactccc accgacgatt ccaacatcga
     1501 aagaaagaaa ctacccgatt tttcgagtaa aacttatggc gttcgcttag gtggccctat
     1561 catcaaggat aaagttttct tttttggcaa cgtcgagatt caaaaggacg aaactcctca
     1621 gcctttcgaa ttttctactt atgaaggcaa ttcggatgta gataaaatta gtcaactaat
     1681 cgaaaaggta aaatcgtatg gttatgaccc gggtggattt cagggagtta taaaaaaact
     1741 cgacggcacc aaaatatttg gacgtgtcga ctggaacatc aatcaaaacc ataagcttac
     1801 ccttcgacat caatatacaa aaggaacaaa aaccgaacct gggtatcaaa ccaataccaa
     1861 catcactttc gaaaatgcgg gtatatattt tccatctacc accaattcat cggctttaga
     1921 actgaaaagt atcttcggaa acaaatttgc caacaacctg attgtgggac ttacctttgt
     1981 tagagatgac cgcgatccta tgggacagaa tttcccttac gtgagcattc gcgacggtaa
     2041 aggcagcatc aatcttggtt ctgaacaatt ttccacagcc aatcagctcg atcaggatat
     2101 tatcactctt accgataatt tcgaatggta ctctggaaaa cataccctta catttggtac
     2161 gcacaacgaa ttctataaca tgtataacct cttcatccgt caaaatttcg gagtttatac
     2221 ctataacagc attgatgatt tcttgaacaa tgatacagct actcaatatg atagaacatt
     2281 ttcgatgaca gatgggaaaa cgggtgatgg ttcgagtgca gctacaaaat tcaaagccct
     2341 tcagttggga ttctatgctc aggacgaata tcaagctctg gataatttga aacttacctt
     2401 tggcctcagg gttgatattc ccatgttttt agacgatccc attgtaaacg aagatttcaa
     2461 taataacatc attcctgttt tagaacagaa tggatgggat ttgcaagggg cacaaaccgg
     2521 tcaaatgcct gatgtccatc tgttatggtc gcccagattc ggattcaact gggatgtatt
     2581 caatgatcaa agtactcagg ttagaggagg tataggtatt ttcacttccc gcatacctta
     2641 tgtttggcca ggtggtgttt acaacaacaa tggacttatg cttggcggca tgagggtaac
     2701 ctatgtttcg aaccctacga gcccttatct gattttcaat cccgattgga acaaccagcc
     2761 cggatggaaa cccggagatc cagttacacc ttcaggccag atcgacctat ttgctaagaa
     2821 tttcaaattc cctcaggttt ggcgtatgag tttggccgtc gaccaaaaac taccttggga
     2881 tgtgattgca actggtgatg ttacttacac caaaaacatt aataatgtac tttataagaa
     2941 ccttgcttat aaaaaatccg gacttcatct gacaggaacc ggcgataacc gccctttatg
     3001 ggaaaaaatt gacctgcagg gtgctaaata tactgacatt atgtgggcaa ccaatacatc
     3061 tctgggatat acttataatc tcactgctca gttgcagaaa accttccata atggcttata
     3121 cacgagttta gcctatacct ttggagcagc tcgctcgatg aacgatggac aatcatccca
     3181 aaactcctct caatggcgcg ttcccaatgt aagagggaaa aatgacctcg acttcagcat
     3241 ctctgatttt gatatgggtc atcgcatcgt gggctatata tcatggaaaa tcgactatct
     3301 gaaacattgt gccacaacta ttggattctt ctattcagga caatcgggtc aaagattttc
     3361 atggggttat caggataata ccaaatcatg gctgggtgaa gataaccaaa gtttggaact
     3421 gatgtatgtt cctaaagatc aaaacgatat tattttactc gatgtcaaag acgccaatgg
     3481 aaatattgtt ataagtaaag aacagcaatg gaatgatctg actgctttca tagaatcgga
     3541 cgattatttg aggtctcgtc gtggcatgta tgccgaacgc aatgctgccc gcaccccctt
     3601 tgaacataac attgaccttc atttggcaca ggacttttat gttaacgtag ccggcaaacg
     3661 aaatacgctt cagattactt tcgatatctt caatctgggt aattttatta attccgattg
     3721 gggtcgtaaa tattatgtat caggatatta tggtaattat cctcttctta acttccaggg
     3781 actcaaagct gatgccgatg gcaataaaac cgtaccaacc tttactttcc agaaacctaa
     3841 aggcgaagta tggaatatcg acgacagcgg tctgataagc tcacgatgga tggggcaagt
     3901 cggaattcgt tatattttca actaatattt ctatttcatg cgccatgaaa aaacctgctc
     3961 aaaagcaggt tttttcttcc gatgcatctc atgcatcttt acaaatttct tacggtgaaa
     4021 gagtgataat agtattgttc agcctcgtta agataaaaat ctataaaata ag
//
LOCUS       DAOORU010000184         3979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_27017_length_3979_cov_3.568298, whole genome shotgun sequence.
ACCESSION   DAOORU010000184 DAOORU010000000
VERSION     DAOORU010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3979
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27017_length_3979_cov_3.568298"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2700
                     /locus_tag="PLF20_09425"
     CDS             <1..2700
                     /locus_tag="PLF20_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HOX81257.1"
                     /translation="KPGGLTFFQGGPYRQVILTNRWQRLSQTPMLISMDAENGPAMRL
                     DSIIAFPNLMTLGALCNDSLIFQIGLAIGQQCKRLGVHLNFAPVVDVNNNPHNPVINY
                     RSIGENPQQVAYNASLLMQGMQQAGIMTTLKHFPGHGDTETDSHNGLPEIKKQIQDLE
                     TNELIPFAYNLSLTDAVMVAHILIPSLDTVNTLPASLSPVIVNHLLREQMGFRGLIIT
                     DALDMKGAGNYKQAGEKEWLAFIAGNDILLLPENIKAAIEKIKNAIDSTIIPYQFLEE
                     KCKRVLAHKYKAGLHDFQPIKTTNLLQDLNQPYYSTIIRDAFKGSLTLINEPVPVPIN
                     PVNYYKILYVPVFAENQAFGEQMKILFPHEKFILPSLADTVAIRQIFDSIKEYDLLIT
                     TIAPPSTFASKNYGLDKDFHSFINALPKNIPGILVLFGCPYIASYFGPLLNHQSIIIT
                     YQNNSITGELAANALYGDNAIRGHLPVSIDNNVSSGTGIETEASQVLGFATPSEVDVD
                     EGKLNKVDEIALEGIKKGAYSGCQILLAHHGKVFYYKSFGYLDVTHRVPVKNSDLYDL
                     ASITKIAATTLVTMKLYDEHKIDNDTELKDLLPNLKNRKLRNTKITDIMYHQAGLFPW
                     IAYYKATLENGVPSKKYYRTQTEPGFEVPVAKNLFLRNDYIDTIISIIDHTPLSQKGL
                     YKYSDLGFILLKFGYENLTKTPFEDYLYTNFYHPLGLSTMVYNPLRWFGHERIAPTLV
                     DTEFRKQLLHGYVHDPAAAMLGGVSGHAGLFAHAFDVARIMQMLLWKGEYNHIRFFQK
                     ETVNYFTSNVEDGNNNRRGLGFDKPPKNFKSSSSSVCSLASPKSFGHSGFTGTYVWAD
                     PDHDLIYVFLSNRIHPSDENEMINKLNIRTRIHQAAYEAIIDY"
     gene            2772..3701
                     /locus_tag="PLF20_09430"
     CDS             2772..3701
                     /locus_tag="PLF20_09430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOX81258.1"
                     /translation="MNHRPHTHNAHTQNTSQQRLGLTIGLNLMIAMVEVVGGIISGSL
                     ALISDSVHNLADSVSLIISYFALKLSKKKVSLRNTFGYHRAEILAAFINATILAGITL
                     FLIVQAFIRFFRPVQIKVDTMGIVALIGLGANLAGMILLKKPSSQSLNIRSSYLHLVG
                     DTLSSVAVIIAALLIELTKLTFIDPLITIIISLYIIKETWSVLQRSTDILMMASPTDI
                     NLLKIQREIEKMEGIDNIHHVHIWQLDDKTIHFEAHLRLAKNPSIHEADQIRQKIERW
                     LIDNNNIQHVTLQIEPSETANACPSNSLLGSTH"
     gene            3709..>3979
                     /locus_tag="PLF20_09435"
     CDS             3709..>3979
                     /locus_tag="PLF20_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81259.1"
                     /translation="MKKNSKSKNALLWIISFIIMTAASVYQRLTGPTHPVQAKIEIEG
                     TSYLFKLIRSHGSKADARIVLSIPDTSIHGIIAYKKINATETWEND"
BASE COUNT         1227 a          861 c          716 g         1175 t
ORIGIN      
        1 aaacccggag gactcacttt tttccagggg gggccttaca ggcaagtaat tttaaccaat
       61 cgctggcaaa gactttcgca aaccccaatg ctgatctcga tggatgccga aaacggtcca
      121 gccatgcgtc tcgacagtat aattgctttc cctaatttaa tgacgcttgg tgctctatgc
      181 aatgattctc tcattttcca gatcggactg gccatcgggc agcaatgcaa aagattgggc
      241 gttcatctca actttgcgcc cgtagtcgat gtgaacaata atccccataa ccctgtcatc
      301 aattatcgtt cgataggaga aaaccctcaa caagtggctt ataacgcctc tctattgatg
      361 caggggatgc aacaggctgg cattatgaca accctgaaac actttccggg acatggagat
      421 actgaaaccg attcacacaa tggattaccc gaaattaaaa aacaaattca agatcttgaa
      481 accaacgaat tgattccttt cgcttataat ctttctctta ccgatgccgt tatggtcgct
      541 cacattttaa taccttcgct cgacacggtg aatacattac ccgcaagcct ttctcctgtc
      601 attgttaatc atttgctgag agaacaaatg ggttttcgag gacttatcat caccgatgca
      661 ctcgacatga aaggagctgg aaattataaa caagctggtg aaaaagaatg gcttgctttc
      721 atagctggca acgatatcct cctattgcca gaaaacataa aagcagctat cgagaaaatt
      781 aaaaacgcta tcgattcaac cataatccct tatcaatttc tggaagaaaa atgcaaacgg
      841 gtattagctc ataagtataa agctggcctt catgactttc aaccaataaa aaccacaaac
      901 cttttacagg atttaaacca accttattat tccactatta tcagggatgc ttttaaaggc
      961 tcacttacgc taatcaatga gcctgttcca gttccaataa atccagtaaa ttattataaa
     1021 attctatatg ttcccgtatt tgccgaaaat caggcttttg gcgaacaaat gaagatttta
     1081 ttccctcacg aaaaattcat actgccttca ttggcagata ctgtggcgat tcggcaaatc
     1141 ttcgatagca tcaaggagta cgacttgctc ataactacca tagcaccccc ttctacgttt
     1201 gcttcgaaaa attatgggct cgacaaagat tttcattctt tcatcaacgc cttgcctaaa
     1261 aatatacccg gaatactagt tctttttggt tgtccttaca ttgccagtta tttcgggcct
     1321 ttattgaacc atcaatcgat tatcatcact taccagaaca attccattac aggtgaactg
     1381 gcagcaaatg cattatatgg agataatgcc attcgcggtc atctcccggt ctctatcgat
     1441 aataatgtat cctcgggtac tggcatagaa actgaagcat cacaggtgct tggctttgca
     1501 actccatcgg aagttgatgt agatgaaggc aaacttaata aagtggatga gatagccctc
     1561 gaaggaatta aaaaaggcgc ttacagtgga tgccagattc tattggcgca tcatgggaaa
     1621 gtgttctact acaaatcatt tggatatctt gatgttactc atcgagtacc tgtaaaaaat
     1681 agcgacttat acgatctggc ttccattaca aaaattgcag ccactacgct ggttaccatg
     1741 aaactatatg atgaacataa aatcgataat gacacggagt taaaagactt attacccaac
     1801 ttgaaaaacc gtaaactgcg aaatacaaaa atcaccgata ttatgtatca ccaagccggt
     1861 ttattcccat ggatcgctta ttataaagcc accttggaaa acggggttcc cagcaagaaa
     1921 tattatcgta ctcaaactga acctggtttt gaagtcccgg tagccaaaaa tttatttttg
     1981 cggaatgatt atatagatac cataatttct attattgacc atactccttt aagccagaaa
     2041 ggtctatata aatacagcga tttaggtttt attttgttaa agtttggata tgaaaatcta
     2101 accaaaacac ctttcgaaga ctatctgtac accaattttt atcatccttt aggtttatct
     2161 actatggttt ataatccttt gagatggttt gggcacgagc ggattgcccc tacgttagtc
     2221 gatacagaat ttcgcaaaca acttcttcat gggtatgtgc atgatccggc tgccgccatg
     2281 ttaggaggtg tttcgggaca tgctggccta tttgctcatg ctttcgatgt ggcgcgtatt
     2341 atgcaaatgc tcctctggaa aggtgagtat aatcatattc gattttttca aaaagaaacc
     2401 gtcaattatt ttacttctaa tgtcgaagat ggaaataata atcgtcgtgg actgggtttc
     2461 gataaacctc caaaaaattt caaatccagc agcagctcag tttgttcctt agcctctcca
     2521 aaaagttttg gccattctgg gtttacaggt acttatgtat gggctgatcc cgatcacgac
     2581 ctgatttatg tttttttaag caatcgtatc catccctccg atgaaaatga aatgatcaat
     2641 aaactcaaca tccgaacccg aattcatcaa gcagcctacg aggctatcat cgattattga
     2701 ccatcaaaga aatattctta aattaactgt catctcgaaa aacttactat aataaaaatc
     2761 gttctcctct catgaaccat cgaccccaca cgcataacgc ccatactcag aatacctctc
     2821 agcaacgtct gggattgacg attggtttaa atttgatgat tgcaatggtg gaagtggtgg
     2881 gaggaattat atcgggttca ttggccttga tctccgactc cgttcataac ttggccgata
     2941 gtgtatcgtt aattatcagt tatttcgccc ttaaattatc taagaagaaa gttagccttc
     3001 gtaatacttt tggttatcat cgtgctgaaa ttttggccgc tttcattaat gctaccattt
     3061 tagctggcat cacactattt ttaatcgttc aggcttttat tcgattcttc cgccctgtac
     3121 aaattaaggt cgataccatg gggatagttg cccttatagg attgggagca aatttagcag
     3181 gcatgatatt actaaagaaa ccttcttctc aaagcctgaa cattcgatca tcttaccttc
     3241 atctcgttgg agatactctt tcgtcggtag cagttatcat tgcagctctg cttatagagc
     3301 tgacgaaact cacttttatc gatcccctga ttaccatcat cataagctta tatatcatca
     3361 aagaaacatg gtcggttctg caacgttcta ctgatattct gatgatggct tctcctacgg
     3421 atatcaatct tctgaaaata caacgcgaga tcgaaaaaat ggaaggcata gacaatatcc
     3481 atcatgtgca tatctggcag cttgacgata aaaccatcca tttcgaagct catttaagac
     3541 ttgcgaaaaa tccttctata catgaagccg atcaaataag acaaaaaatc gagagatggt
     3601 tgatcgataa taataatatc cagcatgtta ctcttcagat cgaaccatcc gaaactgcga
     3661 atgcttgccc ctcgaattca ttattaggtt caacgcattg aatacattat gaaaaaaaac
     3721 tcgaagtcga aaaatgccct tctctggatt atctctttca ttataatgac ggctgcctct
     3781 gtatatcaac gtcttacagg gccaactcat ccagtacagg ccaaaataga aatagaagga
     3841 acttcttatc tcttcaaatt aatccgtagt catggaagta aggctgatgc taggatagtt
     3901 ctttcaattc ccgatacatc cattcatggc ataatagcat ataagaagat caatgccaca
     3961 gaaacatggg aaaatgata
//
LOCUS       DAOORU010000185         3922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_27522_length_3922_cov_4.175071, whole genome shotgun sequence.
ACCESSION   DAOORU010000185 DAOORU010000000
VERSION     DAOORU010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27522_length_3922_cov_4.175071"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2739
                     /locus_tag="PLF20_09440"
     CDS             <1..2739
                     /locus_tag="PLF20_09440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOX81260.1"
                     /translation="QLEEVVIVGYGSTKKMDLTGSITTVKSDKLSYQPAANFASVLQG
                     KVAGVQVTNSGAPGAAPVIRIRGLGTVNSKADPLYVVDGVITDNISFLSPSDIESTTI
                     LKDASASAIYGVKAANGVVIITTKRSTGQKAALSYSGYAGFQTAVNKLPLANNTQYIE
                     LINEKNKLQAEAAGQSFTPLDPANFPDYTDWYDEVMRDQAFVMNHDIGICGGTENSAF
                     YFGAGYFKQDGLLLNQTYERLNIRSSLDSKVNDALKVGYSSTFSTFRTNDAPSVFYDA
                     YVAPPVFKPKVNDTTFTNPVDLGLGNFSNPAASLYYYNSKSNGIRLVGSVFAEVNFLK
                     YFQFRSQYGTDLGYARNRNYTPYYWVSPTQKDTNRTLSRQWTYDYKGVWDNTLTFEDN
                     FGPHRLKVMAGMSAQQENTLGTYGSRMGVPYLGENTLYINLGRAKTSQVNDWGSKITS
                     LSYFGRVNYSLYDRYLLTTTLRYDGSSVFPKDDRWDIFPSVGVGWIITGEEFMKDQNI
                     FDFLKLRASWGVIGNNKIPANVYTLTVEEGGILNTAFGSGQNGNTVIVEGANITSAVP
                     PQLKWEKMNEFDIAIEGKSLDSRLSYEIDFYSRTTKDAIFLLTLSATAGTSGSYYSNN
                     ADILNQGIEITLGWADKVGKFGYDLNLNYAYNHNEVKKLREGTLGLFGGNLPVGGFFS
                     TYTVLGMPIGSYYGRQVIGIFQNQAEVDNYVNDQGKKLQPNAKPGDFKYADIDGNGVI
                     DNKDRVFLGNAIPKSQLGFSAILTYSDFDFTVDLYTRWGNKIYNAKRAQRLGNENYDF
                     DFYENRWHGEGTSNEYPSADLTGDNMLPNSWYFEDGAFLRVRTIQVGYTLPQNFTRKL
                     GISNLRVYINATNPINLFGYNGFNPEIPEGSTTSQGIDLNVYPMSATYNFGINMNL"
     gene            2760..>3922
                     /locus_tag="PLF20_09445"
     CDS             2760..>3922
                     /locus_tag="PLF20_09445"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOX81261.1"
                     /translation="MKTYKIFLSFIAAIVIISFNSCSDYLDLAPEGQLPAEGFFETQE
                     HAMKGVNSIYAHLRAWEMVSFAYIIMQEIPSDNAIKGSEVGDASFINEYDQFLYTPNQ
                     FVLNDYWRGRYRGINLANQCITNIPKIQMDENLKKRLVAETKFLRALIYFDLIRAFGD
                     IPMPLSIPVGSEIYVRTPKAEVYEQIEKDLLDAASILPATYGANDKGRATSGAAKGLL
                     AKVYLYLKDYPKAEQLAMEVINSNVYDLEPNFYKVFRVESENGIESVFEIQAQEIEGQ
                     SDLSFCQHSEVQSVRGQWGWGFNIPTDNLIAAYDAAGDLIRKKVTILFRGDVTPDGDS
                     IQGVTALEGVSGTPRYNGKAYYPSWRQLFGPYGAGQNVRVLRYAEILLIAAEA"
BASE COUNT         1156 a          831 c          852 g         1083 t
ORIGIN      
        1 cagttggaag aagtggtaat agtaggatat ggttcgacca agaagatgga tttaacgggt
       61 tcgattacca cggttaagtc cgataagtta agttatcaac cggcagccaa ttttgcctct
      121 gtcttacaag gaaaggttgc cggtgtacag gttaccaata gtggtgcacc aggtgctgcc
      181 ccagtcatcc gcatccgtgg actaggcact gtaaattcta aggccgatcc tctatacgta
      241 gtggatggcg ttatcaccga taacatcagt ttcttaagcc cttccgatat cgagagcacc
      301 actatcttga aagatgcttc cgcttcggcc atctatgggg taaaggccgc caacggtgtg
      361 gtcattatca ccaccaaacg cagcacagga caaaaagctg ctttatctta ttcgggttat
      421 gctgggtttc aaactgcagt gaataaactt ccattagcta acaatacaca atatatagaa
      481 ctaatcaacg agaagaacaa gttgcaggcg gaggcagccg gccaatcatt caccccactg
      541 gatcctgcca atttccccga ttacactgac tggtatgatg aagtaatgcg tgatcaggcc
      601 tttgtaatga accatgatat agggatctgt ggtggaactg aaaacagcgc attttatttc
      661 ggagccggat atttcaaaca agatggctta ctcttgaatc aaacctatga aaggctgaat
      721 attcgttctt cattagattc gaaagtaaat gatgccctca aggttggata ctcttccacc
      781 ttctctacct ttcgcaccaa tgacgcccct tcggtattct atgatgctta tgtggctcct
      841 ccggtattca agccaaaagt aaacgatacc accttcacca atcctgttga tctgggtctg
      901 ggaaatttta gcaacccagc cgccagtcta tattactata atagtaaaag caatggtata
      961 aggttggtag gaagtgtgtt tgctgaagtt aattttctga aatacttcca attccgttcg
     1021 caatatggga ccgacctggg atatgctcgt aaccgcaatt atacgcccta ttattgggtt
     1081 tctcccactc agaaggatac taatcgtact ttaagcaggc aatggactta cgattacaaa
     1141 ggggtttggg ataacaccct cacctttgaa gacaattttg gacctcatcg cttaaaggta
     1201 atggcaggca tgtcggctca gcaagaaaat actctgggca cttatggttc tcgcatgggt
     1261 gttccctatc ttggagaaaa tacactctat atcaatcttg gacgggctaa aaccagtcag
     1321 gtaaacgact ggggaagcaa aataacttcc ctctcgtatt ttggccgtgt aaattattca
     1381 ctttatgatc gttatctgct aactaccact cttcgttacg atggcagttc ggtattcccc
     1441 aaagatgaca ggtgggatat tttcccctct gtgggtgtgg gatggatcat aacgggtgaa
     1501 gaattcatga aggatcagaa tatattcgac ttcttgaagc taagagccag ttggggtgta
     1561 attgggaata acaagatccc tgccaacgtc tataccttaa ccgtagaaga aggtggcatt
     1621 ttgaataccg cttttggttc aggtcaaaat ggaaataccg tcattgttga aggagccaac
     1681 atcacaagcg cagtacctcc tcaactaaaa tgggagaaga tgaatgaatt cgatatagcc
     1741 atcgaaggga aatcattgga tagtcgactt tcgtatgaaa tcgattttta ttcgagaacc
     1801 actaaggatg ctattttcct gcttacccta tcggctactg caggtacttc gggttcatac
     1861 tactccaata atgccgacat tcttaaccag ggaatcgaaa tcaccttggg atgggcagat
     1921 aaagtcggaa aatttggcta tgacctcaat ttaaactatg catacaatca taatgaagta
     1981 aaaaaacttc gtgaaggcac gctcggttta tttggaggca atttacctgt agggggtttc
     2041 ttcagtactt atacggtttt aggtatgccc attggcagtt attatggccg tcaggttatt
     2101 ggaattttcc agaatcaggc agaagtagat aattacgtaa atgatcaggg taaaaaactt
     2161 cagcccaatg caaagccagg cgatttcaaa tatgcagata tcgatggtaa tggagtgatc
     2221 gacaataaag accgtgtttt tcttggaaac gccataccca agtctcagtt gggattttcg
     2281 gccattctta cttattctga tttcgatttt acagttgatt tgtatactcg ttggggaaac
     2341 aaaatctata atgccaaaag agctcagcga ttaggaaacg aaaattacga cttcgacttt
     2401 tatgaaaacc gttggcatgg cgaaggtact tctaatgaat acccctcggc cgacctcacc
     2461 ggcgacaata tgctccccaa tagctggtat ttcgaagacg gagcctttct acgcgtgaga
     2521 accatccagg tgggatatac cttgccacaa aatttcacca gaaaactcgg aatttcgaat
     2581 ctcagagttt atatcaatgc aacaaatccc atcaacttat tcggttacaa cggattcaac
     2641 cccgaaatac ccgaaggatc aaccacttcg cagggaatcg atttgaatgt atatcccatg
     2701 tcggctactt acaacttcgg tattaacatg aacctataat tttgaaagga ggaaacaata
     2761 tgaaaaccta taaaatcttt ttatctttca tagcagccat cgtaatcatt tcctttaatt
     2821 catgcagcga ttatctggat ttagctcccg aaggacagtt gcctgccgaa ggtttcttcg
     2881 aaacacagga acacgccatg aaaggagtca attccatcta tgctcattta cgtgcatggg
     2941 agatggtatc cttcgcctat attatcatgc aagaaatccc ttccgacaat gctattaagg
     3001 gaagcgaagt aggcgatgct tcctttatca atgaatatga ccaatttctt tatactccca
     3061 accagttcgt cctaaatgat tactggagag gacgctatcg cggaatcaac ctcgccaatc
     3121 aatgtattac taatattcca aagattcaga tggatgaaaa tctgaagaag cggttggtag
     3181 cagaaaccaa atttcttcgt gccttgattt atttcgatct gatacgtgct tttggtgata
     3241 ttccaatgcc tctgagtatt ccggtaggtt cggaaattta cgttcgcaca cctaaagccg
     3301 aggtgtatga acaaatcgaa aaagatctgc tggatgccgc ctcaatttta cctgcaactt
     3361 atggagccaa tgacaaagga cgcgcaacaa gtggagctgc aaaaggtctt cttgcaaaag
     3421 tctatctcta tttgaaggat tatcccaaag ccgaacaatt ggctatggaa gtaattaatt
     3481 cgaacgtcta cgatctcgaa cccaattttt ataaagtttt cagagtagaa tcggagaatg
     3541 ggatagaatc ggtattcgag attcaggcgc aggaaatcga aggacaaagc gacctttcct
     3601 tctgccagca ctccgaagtt caatcggttc gtggacaatg gggatggggc ttcaatattc
     3661 caaccgataa tctcattgct gcctatgatg ctgccggaga tttgattcgc aagaaagtaa
     3721 ccatactctt tagaggagat gttactccag atggtgacag tattcaggga gttactgcac
     3781 tcgaaggagt tagcgggact ccaagatata atggtaaggc atattatcct tcatggcgtc
     3841 agctattcgg accttacgga gccgggcaga atgtaagagt attgcgttat gccgagatcc
     3901 tattgatagc tgcagaagca aa
//
LOCUS       DAOORU010000186         3918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_27558_length_3918_cov_5.228838, whole genome shotgun sequence.
ACCESSION   DAOORU010000186 DAOORU010000000
VERSION     DAOORU010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27558_length_3918_cov_5.228838"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="PLF20_09450"
     CDS             complement(<1..425)
                     /locus_tag="PLF20_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOX81262.1"
                     /translation="MIYRSKAPLRIGLAGGGTDVSPFCDLYGGAVLNATINLYARASI
                     ICDDSSHIVLHAIDRGFRREYALSEEIIPEKGLELHAGVYNRIVRQFTHQPVAMRLET
                     YVDVPPGSGLGTSSALVVAILGAFTEMLSLPLGEYDIAHL"
     gene            complement(1084..2223)
                     /locus_tag="PLF20_09455"
     CDS             complement(1084..2223)
                     /locus_tag="PLF20_09455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOX81263.1"
                     /translation="MTDQSDIEIFILGTTYPLRGGLATFNERLAQAFKDAGYRVTIYN
                     FSLQYPSLFFPGKTQYSTDPPPVGLNILTKVNSINPFNWIKVGREIKNKRPLLLVLRY
                     WMPFMAPCMGTIARIVKKNHFTKIVAIIDNIIPHEARFFDTCLSKYFLGSCHGFVAMS
                     KSVLEDIGRFGNRKPRKFYPHPLYDTYGKPLSREEACQKLDLDPSFRYMLFFGFVREY
                     KGLDILLQAFTDKRFEKLPVKLIIAGEFYTNAEPYRKMISDLNLSERIVMHNSFISNS
                     EVPAWFCASDIIVQPYKSATQSGVTQIAYYFEKPMIVTNVGGLPEMVPDGKVGYVVEP
                     NPKSLADAIYKFYSLGNTNEFVQHIKKEKQKFTWEGLIRTFEEFL"
     gene            complement(2207..3157)
                     /locus_tag="PLF20_09460"
     CDS             complement(2207..3157)
                     /locus_tag="PLF20_09460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOX81264.1"
                     /translation="MDISIIIPLYNEAESLTELHAWITKVMQQNGFSYEIIFIDDGSN
                     DDSWKIIEALNHDDPHVKAIKFRRNYGKAAALNTGFEAAIGEVIITMDADLQDSPDEI
                     PELYQMIKNQGYDLVSGWKKKRYDPPSKTIPTKLYNWATRKATHIKLHDFNCGLKAYR
                     NEVVKAIEIYGEMHRYIPVIAKWAGFVKIGEKVVQHYPRKHGKSKYGLNRFINGFLDL
                     ISISFIGRFGKKPMHFFGLIGTALFFIGFLIACYLAYAKFFLAEYKMTQRPLFYFGLL
                     SMILGTQLFTAGFVGELISRTSHDRNKYLIEKRTGFDRSI"
     gene            complement(3157..3720)
                     /locus_tag="PLF20_09465"
     CDS             complement(3157..3720)
                     /locus_tag="PLF20_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HOX81265.1"
                     /translation="MKNKLILTLNNSLKYGALMGVVFILLSLIYYIAGFDQFNTVFGI
                     VNLLISLAMYIGFLYYSNKILRDKKLNGILTYTDGLLNSFFTGVIAAIVGGIYSYVFY
                     QYFDPSYFTEAIERSIEMLEQNPALTGEALDSAIARVESVTPLKSALQGFYGNIAMSL
                     IFALIISAFTRKKPIPSFSDEEISTHD"
     gene            3828..>3918
                     /gene="ffs"
                     /locus_tag="PLF20_09470"
     ncRNA           3828..>3918
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLF20_09470"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
BASE COUNT         1238 a          732 c          768 g         1180 t
ORIGIN      
        1 agatgagcga tgtcatattc tcctaaaggc aatgaaagca tctcagtaaa agctccgagt
       61 atggctacta cgagtgctga cgaagttccc aacccagaac ctggcggcac atcgacgtaa
      121 gtttctaatc gcatagcaac aggttgatgc gtaaactgtc taactattcg gttataaact
      181 cctgcatgaa gttcgagccc cttttcggga ataatttctt ctgatagagc atactctctg
      241 cgaaaacctc tgtcaatggc atgtaagaca atgtgcgatg aatcatcgca aataatggaa
      301 gctcttgcat aaagattgat ggtagcattg agcaccgctc ctccatatag atcacaaaaa
      361 ggagatacat cggttcctcc tcctgcaagg ccaattcgta atggagcttt acttctgtat
      421 atcatttgcc taggttttgt caaatatatt ttttgaagat atttcgtgta actttcattc
      481 gttgaaatga gaatgtaaaa ttacgacctt ttcggtaatg aagggctgac tgactttagt
      541 tccattgcat gcgtcggaaa ctcaatttta ttgcctcaac atttatttat tgagcaagcg
      601 atcgattttt tcgagaatat actccttgca gaagtgcatg ctgatgtaat tgttacatct
      661 ggcttccgag gtttgtcacg gttgtgttcg caagtataag ttgtttgtgc cagaatgtac
      721 accttggata aattaaaagg ttttaacatg catgataacg gctgtggcaa atgatatact
      781 ctgcatcatg attctcaact tttacctgtc gagaaaaatt aaggcgacat agaagacgat
      841 catggtgaga ttgtatgata tcaatgatca tcatcaagga aaagtttaga attttacaaa
      901 cccatgaaat tattctatgg aaatttttta tggtattttt ttgaaacgaa ttattatgcg
      961 aatatcgagg ataaaattga tctacaaagc gaaaaggcca caatacataa cgaaattcaa
     1021 gacggaaaag atttcgataa ttgcttgaaa atcaattgaa tgtaaaaatg gttcatttcc
     1081 ttgttataga aattcttcga aagttctgat gagtccctcc caggtaaact tttgtttttc
     1141 tttctttata tgttgaacga actcatttgt attacctaag gaatagaatt tgtaaatagc
     1201 atcggctaaa gatttagggt tgggttcaac tacatagcct actttaccat cgggtaccat
     1261 ttcgggcaag cctccaacat tagttactat catgggtttt tcgaaataat aagcaatctg
     1321 ggttacacca ctctgagtag ccgatttgta aggctggaca atgatgtctg aagcacaaaa
     1381 ccaggcagga acttcggaat tgctgataaa agaattatgc atcacgattc gttcactaag
     1441 attcagatcg cttatcattt ttctataagg ctcggcatta gtgtaaaatt ctccagcaat
     1501 gatcagtttt acgggtagtt tttcgaaacg cttgtcggtg aaggcctgta aaagaatatc
     1561 gagtcctttg tattcacgaa caaagccaaa aaacaacata tacctgaaag atggatcgag
     1621 atcgagcttt tgacaggctt cctcacgcga aaggggcttg ccataagtat cgtataaagg
     1681 atgaggataa aattttcgtg gtttcctatt gccgaagcgg ccaatatctt caagcaccga
     1741 cttcgacatg gccacaaagc catggcaact tcccaaaaaa tatttcgata agcaggtatc
     1801 gaaaaaacgt gcttcatgag gaataatatt gtcgatgatg gctacgattt tggtaaaatg
     1861 attttttttg acgattcgtg caatcgtacc catacaaggt gccataaaag gcatccagta
     1921 acgaagaacc aacagaagag gtcgtttgtt tttgatttcg cgtccaactt taatccagtt
     1981 gaagggattt atagaattga ctttagtgag aatgttcaac ccaacaggcg gaggatcggt
     2041 agaatattga gttttgcccg gaaagaataa agaaggatat tgaagactaa aattgtagat
     2101 agtaactcta tatcctgcgt ccttaaaagc ctgcgcaagt cgctcgttga aggtggctaa
     2161 tcctccgcgt aaaggatagg tggttcctaa aataaatatt tcaatatcag attgatcggt
     2221 caaatccggt tcgtttttcg attaaatatt tatttctatc gtgcgatgtt cgggaaataa
     2281 gctctcctac aaatcctgct gtaaacaatt gagttcccaa tatcatcgaa agaagtccga
     2341 aatagaataa aggcctctgg gtcattttat attccgccag gaaaaattta gcataagcca
     2401 gatagcaggc tataagaaat cctatgaaaa acagagctgt acctatgaga ccgaaaaaat
     2461 gcatgggttt tttcccaaac cttccaataa aactaataga aatcaaatcg agaaatccat
     2521 tgataaatcg atttagtcca tacttacttt tcccatgttt acggggatag tgttgaacga
     2581 ctttttctcc aatctttaca aacccagccc atttggcaat aacaggaata taacgatgca
     2641 tttctccata aatctcaata gcttttacca cctcgttgcg gtaggccttc aatccgcagt
     2701 tgaaatcatg taacttgata tgtgtagctt tgcgagtagc ccagttataa agttttgtag
     2761 ggatagtttt tgaaggcgga tcatatcgtt ttttcttcca acccgatacc aaatcatatc
     2821 cttgattttt tatcatttgg taaagctcgg ggatctcatc ggggctatct tgcaaatcgg
     2881 catccatagt gatgattacc tcacctattg ccgcttcgaa acccgtattt aatgctgcgg
     2941 ctttaccata attcctgcga aacttgatgg cttttacatg cggatcatca tgattcaaag
     3001 cttcgatgat tttccatgaa tcatcattgc tgccatcgtc aataaagata atttcgtaag
     3061 aaaatccgtt ttgctgcatt accttagtaa tccaggcatg caattcagta agcgattcag
     3121 cttcgttata taaaggaata atgatagata tatccatcaa tcgtgggtac tgatttcttc
     3181 gtcagaaaaa ctgggaatgg gtttttttct ggtaaaggca gaaataatca gtgcaaaaat
     3241 aaggctcatg gcaatattcc catagaatcc ctgcaaagcc gattttaaag gagttacact
     3301 ttctaccctt gcaatagcac tgtccagagc ttcgcccgtc aaggcaggat tctgttccag
     3361 catctcgata ctgcgttcaa tagcttctgt aaaataggat ggatcaaaat attggtaaaa
     3421 tacataagaa taaatcccac caacgatagc tgcaataact cccgtgaaga aagaattcag
     3481 caaaccatcg gtgtaggtca aaatgccatt cagcttttta tctcttagga ttttattact
     3541 gtaataaaga aatccaatgt acatagccag agatattaga agatttacta ttccaaaaac
     3601 agtattaaac tggtcgaatc cggcaatgta atatatcagg ctgaggagaa tgaaaacaac
     3661 tcccataagg gctccatatt tcaaactatt gttgagcgta aggattaatt tgtttttcat
     3721 agtttaaagg tatttttact tattgagcaa aggtaaaaat attcacgaaa ttgtaaaaat
     3781 ctttcgataa agaataaaaa attagaattt tgttgtattt ttgcaaaggg taagtcttat
     3841 acgaccagct cccgatgaac tcccccaggt ccgggaggaa gcaagggtaa tcggtcgtag
     3901 cggtgcgata taaggagc
//
LOCUS       DAOORU010000187         3851 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_28205_length_3851_cov_5.222866, whole genome shotgun sequence.
ACCESSION   DAOORU010000187 DAOORU010000000
VERSION     DAOORU010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28205_length_3851_cov_5.222866"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..295)
                     /locus_tag="PLF20_09475"
     CDS             complement(<1..295)
                     /locus_tag="PLF20_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81266.1"
                     /translation="MVISVCVDVSNWATKTPLASIRKIVQDDRYFFKIKPGLWALNEY
                     KSEVLKKFNLEKSVENNVDFNHSYYQGLLLEIGNLKKDFKTYIPLQDKNKLFLN"
     gene            550..1377
                     /locus_tag="PLF20_09480"
     CDS             550..1377
                     /locus_tag="PLF20_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOX81267.1"
                     /translation="MMNTSIEQLRLTAKSIRIWVVKSLAAAGSGHLGGSLGMADVLTM
                     LYFKVLRHDPKNPFWSCRDRFILSIGHVAPVLYSTLAHAGYFPLEELLTLRKLGSRLQ
                     GHPGKEYGLPGLELSAGSLGQGLSVSVGLSLGFKLDKLDNKVYCLLGDGELQEGSVWE
                     AAMAAGHYHLDHLVAIVDRNGVQIDGPTEKVMRLEPLRQKWEAFGWEVTECDGNNLEE
                     LSLAFEKIGQESSKPKVLIARTIMGKGVSAIEGDYHWHGKAPSQAQTEEFINEILSK"
     gene            1374..2345
                     /locus_tag="PLF20_09485"
     CDS             1374..2345
                     /locus_tag="PLF20_09485"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HOX81268.1"
                     /translation="MNSFQLKGLKATRAGFGEGVVEAGRRNNDVVVIGADITSSVGLD
                     GFAREFPDRFFSMGIAEQNGMGVAAGLALEGKIPVFSTYAVFSAFRAADQLRISVCYN
                     NLHVIIGGAHAGISVGPDGATHQALEDLAYVRALPNITVLSPCDASQAFKATLSALLE
                     IAGPVYIRFGRENVPDFTTYDLEFIPGKAQVFRPGKDVTIIATGHLVWPSIEASELLA
                     KQGISARVINLHTIKPIDEETIIRAAAETGCILTAEEHQIHGGMGSAVAEVIVRDHVV
                     PMEMIGINDTFGESGQPDELMKKFGLTAENIAQKTTQLLKRKKITNS"
     gene            2345..2950
                     /locus_tag="PLF20_09490"
     CDS             2345..2950
                     /locus_tag="PLF20_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81269.1"
                     /translation="MNTFIVLLQSTIPSLLVLAVAVYFFHASNEQFLKRWKDTVPLLK
                     DDRTERLLKSFIEREEKLQLLSIKKENQRITLPLRLQAYERFVLLLERITLSRLLLRV
                     AEPALTAQQLQHSLILNIRNEFEHNLSQQIYISETSWEWIKKTVEESIQLINTSASTI
                     PADSSASVLISLLMEMENSGQAPQTHKVISHLHAEAQFLLA"
     gene            complement(3392..>3851)
                     /locus_tag="PLF20_09495"
     CDS             complement(3392..>3851)
                     /locus_tag="PLF20_09495"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX81270.1"
                     /translation="LISGNGILKSYQVPLNTLQHTGKAVLAIVSGFYHPENNSNGQAL
                     GLYLIPAEGGAYIPLLDVTGTSEIQNDDNIKIYPNPAYDFIEVSVNNLAGPMILELYS
                     LEGKKIWSKNWHIAFAAKEKFDVSELPWGMYFVRIITDNSVTIKKLQITK"
BASE COUNT         1064 a          762 c          840 g         1185 t
ORIGIN      
        1 tatttaaaaa caacttattc ttgtcctgca atggtatgta agttttaaaa tcttttttta
       61 aattaccaat ttccaataat aagccttgat aataggaatg gttaaaatca acattattct
      121 caacagattt ttctaaatta aatttcttta aaacttcaga tttatattca ttcagagccc
      181 aaagtcccgg tttgattttg aaaaaatatc tatcatcttg aacaattttt cttattgaag
      241 caagaggtgt tttagtagcc caatttgaaa catcaacaca cacactaatc accatccact
      301 atccactatc cactatccac cagtctcctc gtctcccagt ctccctgtct cctttgctcc
      361 cttcttccgt tttaaacttt ataaactttt aacttaccaa tcgccattca ccatcctccc
      421 gtcccctcat cgcctcgtca ccccgtctcc tcgtctcctc gtctcctcgt ctcccttcct
      481 cccttcttca tattttctct cgtcttgtat tatttttcga aatttttcta attttggcta
      541 agcaatttga tgatgaatac ctctatcgaa caacttcgcc ttacggcaaa atctatccga
      601 atatgggtgg tgaagagtct ggcggcggct gggtcgggtc atttgggtgg gtctctgggc
      661 atggccgatg tgcttaccat gctttatttc aaagtattga gacacgatcc taagaatccc
      721 ttctggtctt gtcgtgaccg atttattctt tctattggcc atgttgcccc ggtattatat
      781 tctactcttg ctcatgcagg ttattttcct ttggaagaac ttttgacgct gaggaaatta
      841 ggaagtcggt tgcaaggtca tccaggtaag gaatatggtt tgccggggct ggaactttcg
      901 gcgggttcgc tgggacaggg gttaagcgta agcgtaggtt tatcgctggg ttttaagctc
      961 gataaacttg ataacaaggt ttattgttta ttaggtgatg gtgagttaca ggaaggttct
     1021 gtttgggaag cggcgatggc tgctggtcat tatcacttgg atcatttggt ggctattgtg
     1081 gatagaaatg gagtccaaat agatggcccc acagaaaagg tgatgcgttt agaaccttta
     1141 cgtcagaaat gggaagcctt tggatgggaa gttaccgaat gtgatggtaa caatttggag
     1201 gaattatctc tggcctttga aaagattggt caggaatcat cgaagccgaa ggttttgatt
     1261 gcaaggacca ttatgggtaa aggtgtatcg gccatcgaag gtgattatca ctggcatgga
     1321 aaagcgccaa gtcaagctca aaccgaagaa tttattaatg aaatcctcag caaatgaata
     1381 gtttccaatt gaaagggttg aaggctacgc gcgcgggttt tggtgaaggg gttgtcgaag
     1441 ccggtcgaag gaataacgat gtagtggtga ttggagcgga tatcacttcg tcggttggtt
     1501 tggatggttt tgcgcgggaa tttcccgatc gttttttttc tatgggtatt gccgaacaaa
     1561 atggcatggg agtagctgca ggattggccc tcgaagggaa gattccggta ttttcgactt
     1621 atgctgtatt ttctgctttt agggcagctg atcagctcag aatttcggtt tgttacaata
     1681 atttacatgt aataataggt ggggcacatg ccgggatatc tgtgggaccc gatggagcta
     1741 cacatcaagc gctcgaagac cttgcttatg ttcgcgcttt gccgaatata acggttctga
     1801 gtccctgtga tgctagccag gctttcaagg caactctatc ggctttattg gagatagcgg
     1861 ggcctgttta tatacgattt ggaagggaaa atgtacccga ttttacaact tacgatctgg
     1921 aatttatccc agggaaggct caggtttttc gtccgggaaa ggatgttacc atcattgcta
     1981 ctggccattt ggtatggccg tcaatcgaag cttcagaatt gcttgctaaa caggggattt
     2041 cagccagagt catcaatctg catactatta aaccgatcga tgaagaaacc atcatcaggg
     2101 cggccgcaga aacggggtgc atactcactg ccgaagagca tcagatacat ggaggaatgg
     2161 gtagtgctgt ggctgaagtg atcgtgagag atcatgtcgt acctatggaa atgatcggaa
     2221 ttaatgatac tttcggagaa tcgggacaac ccgatgaact gatgaaaaaa ttcggcctga
     2281 ctgcggaaaa tattgctcaa aaaacgactc aattattgaa aagaaagaaa ataacaaact
     2341 cataatgaat acattcatag ttttattaca aagcactatc ccttcattac tggtgttggc
     2401 cgtagcggtt tactttttcc atgcttctaa cgaacaattt ctgaaaaggt ggaaagacac
     2461 agtacctctc ttaaaggatg accgaacgga aagattgttg aaatcattca tcgagagaga
     2521 agagaaatta cagcttttgt ccatcaaaaa agaaaatcag cgtattacac ttcctttacg
     2581 attacaggct tacgagcgtt ttgtgctctt gctcgaacgt attacgttat cacgcttgct
     2641 actgagagtt gcagaacctg cattgactgc ccaacagctt caacattccc ttattctgaa
     2701 cattagaaat gaatttgagc ataacctttc gcaacagatt tatatttcgg aaacatcgtg
     2761 ggaatggatc aaaaaaacgg tagaagaaag tattcaattg atcaatactt cggcttcgac
     2821 aatacctgcc gattcatcgg cttcggtttt gatttcgctg cttatggaga tggaaaattc
     2881 cggccaggca cctcagactc ataaagtcat cagccaccta catgccgaag ctcagttcct
     2941 tttagcctga ggatagattt ttatatcaca ggatttgatg accataccac tgatatctac
     3001 catagacttt agtgtgataa tttaaatcgg taaaagaagc gggtagaatt atcagagttg
     3061 ggatcattat caacgcactc catattttgt aaaatctcga attttcttag ccattgatga
     3121 tggtgtttta ctcgaacaga atccgaatgg ataagctcga ccaattgagt atcagaatgc
     3181 ttatcatctt gcaatgattg tgtaatgaag acatttttac tggtacattt ccggattggc
     3241 tttatggtag cttggcataa accttagttt tgttcataag aattgggagt tttaactctg
     3301 agagttcggg ggaaaaggtt tcgggaaagg ctatgtgcga tatgattgag ccccgccacg
     3361 aagtggcggg gctcaatcat cacaagctcg attatttggt tatttgcaat ttttttattg
     3421 ttaccgaatt atcggtaatt attcttacga aatacatacc ccaaggcaat tccgaaacat
     3481 cgaacttctc ttttgcagca aacgctatgt gccaattctt gctccatatt tttttacctt
     3541 cgagggagta taactccaaa atcattggtc cagcaagatt gttgacactt acttcgataa
     3601 aatcataagc cggattggga taaatcttaa tgttgtcatc attttgtatt tcgctggttc
     3661 ctgtaacatc cagcaatgga atataagctc cgccttcggc tggaatcaaa tataaaccga
     3721 gagcctgccc attgctgttg ttttcgggat gataaaatcc agaaacaatg gctaaaactg
     3781 ctttaccagt atgctgtaaa gtatttaatg gaacctggta actctttaga attccatttc
     3841 ccgagatgag a
//
LOCUS       DAOORU010000188         3772 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_28998_length_3772_cov_6.051386, whole genome shotgun sequence.
ACCESSION   DAOORU010000188 DAOORU010000000
VERSION     DAOORU010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28998_length_3772_cov_6.051386"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            432..3032
                     /gene="clpB"
                     /locus_tag="PLF20_09500"
     CDS             432..3032
                     /gene="clpB"
                     /locus_tag="PLF20_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOX81271.1"
                     /translation="MNLNNFTVKSQEIIQEAQFLAQEKQHGQIDTGHFLVALLEKDEQ
                     VTPFLLKKIGANPSFILQEGQKLLNTYPRVSGGELLLSRESSQLLQRAISIAREMGDD
                     YVAIEHLLLSLVAGNDAVASILKSQGVGRKDLEEAIQQMRKGSKVDSVTSDENYNALN
                     RFAINLNEMARKGKLDPVIGRDEEIRRVLQILSRRTKNNPILLGEPGVGKTAIVEGLA
                     FRIINGDVPENLKTKQIYSLDTGALIAGAKYKGEFEDRLKAVIKEVTSSEGEIILFID
                     EIHTLVGAGGGEGAMDAANIMKPALARGELRAIGATTLKEYQKYFEKDKALERRFQTV
                     MIDEPDAASAISILRGLRERYENHHKVRIKDEAIIAAVELSQRYITDRFLPDKAIDLI
                     DEAAAKLRLEINSLPEELETVERRIRQLEIEREAIKREGDEEKLEQLNQEIANLTEER
                     NVLRAKWQSEKNLIDQIQQKKREIEDYRLAAEQAEREGNYGRVAELRYGKMREAEQQI
                     EHLRKQLAEIQGDKPMINEEVSAEEVAEVVSKWTGIPVSRMLQSEREKLLHLEEHLHK
                     RVVGQDEAISAVADAIRRSRAGLQDPKRPVGSFIFLGTTGVGKTELARALAEFLFNDE
                     NNMVRIDMSEYQERHSVSRLIGAPPGYVGYEESGQLTEAVRRKPYSVILLDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRTANFKNTVIIMTSNIGSHLIQERIPENAKEIDPKILE
                     QTREEVMDLLKKTIRPEFLNRVDEIIMFKPLTFDEVIQIVRLQFEHLAETLAANEIQL
                     KYTEKAVEYIAKAGFDAQFGARPIKRAMQREILNVLSKKILAGDVDKDLIIVADATAE
                     GIVFRNEPNK"
BASE COUNT         1239 a          685 c          824 g         1024 t
ORIGIN      
        1 aataaactaa tcgaactgac acaactttac gatagagatt gaaaaactat tgtcgagcct
       61 ctttcaggaa ttttccctct tatatcatga attcatcatt ttttgctgat tcttcgatat
      121 ttttgtgaag ttatacgatt aggatttttc catagactaa tctactatca aaatagaacg
      181 agaaagtgaa tggcttgaag attttttgaa atctgataaa actgactgct tttgagtttt
      241 ttaagttata gaaaagattt acaataatga cattttgtct tatttttaga agcaatttta
      301 tcgatttata agacattttg tcataattat atcgcatagg gactcgataa taagaatggc
      361 aagccaattg agtaaagttg ggaaaaacaa agattagatg aaattagata aaacaggaag
      421 gagcaaaaga tatgaatctc aataatttta cggtcaaatc gcaggaaatt attcaagaag
      481 cccagttttt agcacaagaa aaacagcatg ggcaaattga cacggggcat tttttggtag
      541 ccctgctcga aaaagacgaa caagttactc catttttatt gaaaaaaatt ggagcaaatc
      601 catcgttcat acttcaggaa ggacaaaagc tgttaaatac ttatccaagg gtaagtggag
      661 gcgagttgtt actatcgcgt gaaagcagtc agttgttaca gcgagccatt agcattgccc
      721 gtgaaatggg agatgactat gtggccatag aacatctttt attatcgctg gtagctggca
      781 atgatgccgt agcctcgata ttgaaatcac agggtgttgg tcgcaaagac ctcgaagaag
      841 ccattcagca gatgcgcaaa ggctcgaagg tggacagtgt gacttctgat gagaattaca
      901 atgccttgaa tcgttttgcc atcaatttga atgaaatggc acgaaaaggg aagctcgatc
      961 ccgtaattgg gcgagatgag gaaataaggc gtgtgttgca gattctttcg cgacgcacca
     1021 aaaacaatcc catcttgtta ggagaacctg gtgttggaaa aacggctatc gtagaaggat
     1081 tggcttttcg aatcatcaat ggcgatgtgc ccgaaaatct aaaaaccaaa caaatctatt
     1141 ctctcgacac gggtgccttg atagcgggtg cgaaatacaa gggagaattc gaagatcgtt
     1201 tgaaggctgt cattaaggaa gttaccagtt cggaaggtga gatcatttta ttcatagatg
     1261 aaattcatac attggtagga gccggtggag gcgaaggtgc catggacgcc gcaaatatta
     1321 tgaagcctgc tcttgcgcga ggcgaattgc gggcaattgg tgccactacc ttgaaagaat
     1381 atcagaaata tttcgaaaag gacaaggcat tggaacgtcg tttccaaaca gtgatgatag
     1441 atgaacccga tgcagcttcg gctatttcta tcctcagagg cttaagagaa cgttacgaaa
     1501 atcatcataa agtcagaata aaggatgaag ccatcatcgc tgcagtcgag ttgtctcaac
     1561 gttatattac cgatcgtttt ctacccgata aggccatcga cctgatcgat gaagctgcag
     1621 caaagttaag gctcgaaatc aattcattgc ccgaagaatt ggaaacggtg gagcgtcgga
     1681 ttcgtcagct tgagattgaa agagaagcca ttaagcgtga aggcgatgaa gaaaagctcg
     1741 aacagctcaa tcaggaaatc gccaatctta ccgaggaacg caatgtattg agagccaaat
     1801 ggcaatcgga gaaaaatctg atcgaccaaa tccagcaaaa gaaaagagag atcgaagatt
     1861 atcgccttgc tgccgagcaa gccgaaagag aaggaaacta tggaagagtg gccgaactcc
     1921 gatatggtaa gatgcgagaa gccgaacaac agatcgagca cctcagaaag cagctggcag
     1981 aaattcaggg cgataaaccc atgatcaatg aagaagtaag tgccgaagaa gtcgctgagg
     2041 tggtgagcaa atggactggc ataccggtaa gtcgaatgtt gcagagtgaa cgtgaaaaac
     2101 tgcttcatct cgaagaacat ctgcataaga gagtagtcgg acaggacgaa gccatcagtg
     2161 cggtggccga tgctatccga cgtagccgag ctggactgca ggatcccaaa cgaccggttg
     2221 gttcgttcat cttcctagga actactggag tgggtaaaac cgaattggct agggctctgg
     2281 ccgaatttct gttcaacgac gaaaataaca tggtaagaat tgacatgtcg gagtatcagg
     2341 aacgtcattc ggtttcgcgc ttgattggtg ctccaccagg atatgtgggt tacgaagaaa
     2401 gtggccagct taccgaagcc gtgcgtcgca aaccttattc ggtcattctg ctcgatgaaa
     2461 tcgaaaaggc acatcccgat gtgttcaata tactcttaca ggtactcgat gacggaaggc
     2521 ttaccgataa taaaggacgt acagccaatt tcaagaatac cgtcatcatc atgacctcta
     2581 atattggctc gcatttgatt caggaacgta ttcccgaaaa tgctaaggaa atcgatccta
     2641 agattttaga acaaacccga gaagaagtca tggatttgct caaaaagacg attcggcccg
     2701 aattcctcaa cagggtggat gaaatcatca tgtttaaacc cttgacgttc gacgaggtaa
     2761 ttcaaattgt tagattacag ttcgagcatt tggccgaaac actggcagcc aatgaaatac
     2821 agttgaaata taccgaaaag gctgtcgaat atattgcaaa ggctggtttt gatgcacagt
     2881 ttggcgctcg acctattaaa agagccatgc agcgtgagat tctcaacgtg ctgtcgaaga
     2941 aaattctcgc cggcgatgtg gataaagacc tcattattgt ggccgatgct acagcagaag
     3001 gaatagtgtt ccgtaatgaa ccgaacaaat aaaaccaata aaatttaatc tcttggttgt
     3061 gaggatagta tgtgttaggc gtgctatcct cattttttta tttcgaattc tcatccctcg
     3121 aagaggcttt cttgaaaaac gaaaacgggg gagggttccc ccgtttctcc aaactttaac
     3181 caaaaacaat ttacaaacca atgaaaacaa aatcttatga ttcagtttca ttccgatgca
     3241 aaactaaagg tatattatga actttccaaa taatgttctg aatatttttg tatatcaact
     3301 ctaatttatt atcatccaac gataaatgtt tttatgaata ttgcctgaac ggcaaaatca
     3361 atatttctga ttaaaaatcg taacaaattg attttcttta tcatataagt atttatataa
     3421 aatgttctca catggaaaca ttaaggagat tttgagttcc gagctaaatt tatcaatatt
     3481 caataaaaat ctaaacagtt ttgctaaaga atttagttca aattcattaa acgtttagtt
     3541 cattgcattc tttcttctgt aaatcttcga aacgtcttca tgatttctgc ttcatttgca
     3601 tcttgaaata tgcatttctg caaagccgga ggccaaaaac gggtattgaa tattacttat
     3661 cccaacttga gcattttacc ctgaaaaaac aggaaaaaaa tagagttttt gctatgattt
     3721 taaatgttat gttactgatt attaaatatt taaattttta ataatttttg ta
//
LOCUS       DAOORU010000189         3740 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_29313_length_3740_cov_5.744098, whole genome shotgun sequence.
ACCESSION   DAOORU010000189 DAOORU010000000
VERSION     DAOORU010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3740
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29313_length_3740_cov_5.744098"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="PLF20_09505"
     CDS             <1..1482
                     /locus_tag="PLF20_09505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOX81272.1"
                     /translation="TLDPYTILIPEDQIQNYRFFMSGEYGGIGITSLQRANGDCTIID
                     VKEDSPASRAGLKPGDLIVKINNIDLKDKTDEDIDILIKGEVGSPISLSIMKYKSNQI
                     EKLTLLRENIQLPNISYSGMIGDDIGCIRLEQFTDKSSKEFYEAFQKLRQSKNLKGLI
                     IDLRGNGGGLLDQAITIVNQFVDKGLLIVSTRGRNIDRNRNYYTTNPPLDLSIPLVVL
                     VDKYTASASEILAGALQDLDRAVVIGEKTFGKGLVQNILPLPYNNQLKITIAKYYIPS
                     GRCIQAIDYFKHDQNRTSVQTKPVFYTHNGRMVFEQGGIEPDLPSDSVSNNPLINSLF
                     QNFIIFDFVNEFAYQHPHIDPLEKFTLDNKIFNDFIQFTTDRYKDWDYENINLMQQLR
                     NQLSAQNRLDNEIKSSLDKIELQLYDQIPILLSQTREDLSFPLMSEIISRYTKPQDRI
                     KFLITNDPEITKAANLLRDMTTYQSILAGTHPNCHNRLRPANP"
     gene            1487..3055
                     /locus_tag="PLF20_09510"
     CDS             1487..3055
                     /locus_tag="PLF20_09510"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOX81273.1"
                     /translation="MTREKLFYYIYLLGFMSLVVAIPTSNYVMSLSQIIMIGAWLFYG
                     HYKIGLKQLLNKKIAWVFISFYLLHILGLIYTTDFAYAIKDLRTKLPLLVLPFVIFTM
                     PRPSNKFLQLIVWLHALAIFLASLIGIYLAWQKGYVNYRLYSPGISHIRFGLNICLSA
                     VLLLYDIIHRKKLIPRILASLLIAWYIVYLSLFGSVTAIVILGILLVIGISIFIIRHS
                     PVWGKIIYSFILTASILGFSFLIKETLQLILHHSKEKIDFANLEKFSADGHAYLHDTV
                     YFGKEFGQYVGLYYCPDELPQAWAKRSKLDYYGKDLAGQPLMATIVRYLNSKGLRKDA
                     RGLAQLSDEEIGWIEKGVANAQYMQSNALKRTIMEFALGYEHFKEGSGTRGNSIIQRF
                     ELWRASIEIIKKNPVIGVGTGDVPQAFKQQLMAMHSDLSHTSLRSHNQYLSITIAFGI
                     IGLLWFLWILLYPLKINQLYKDWRYVAFLIIMAVSMINEDTIESQAGITYYVFFSLFL
                     YLLSIEKETMNNFP"
     gene            3076..3564
                     /gene="cdd"
                     /locus_tag="PLF20_09515"
     CDS             3076..3564
                     /gene="cdd"
                     /locus_tag="PLF20_09515"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOX81274.1"
                     /translation="MKTLTLITEIEEFDSVDQLPEKDKELLLEAREAASKAYAPYSNF
                     KVGAAIRMGNGKIVQGSNQENSSYPVGCCAERTALFYASSCFWGEKICAIAIASQTTN
                     SDGTRPLSPCGMCRQAILEYEILQQAPIRILMLSGSTKVWIAPSAVSLLPFQFDHQYL
                     EQ"
BASE COUNT         1170 a          790 c          692 g         1088 t
ORIGIN      
        1 actctggacc cttatacaat attaatacca gaggaccaga tacagaatta cagattcttc
       61 atgagtggag aatatggagg aattggcatc acaagcttgc aaagagccaa tggcgattgt
      121 accatcattg acgtgaaaga agacagcccg gcttcacgag ctggcttgaa accaggcgat
      181 ttgattgtga aaatcaacaa tatcgacctg aaagataaaa ccgacgaaga catcgacatt
      241 cttataaaag gagaagtagg aagcccaata tctctctcca taatgaaata taaaagtaat
      301 caaatcgaga aattgactct gctgagagaa aacattcaac ttcccaacat ttcctattcc
      361 ggaatgatag gcgacgacat cggctgcatt cgtctggaac aatttactga taaatcatcc
      421 aaagaatttt acgaggcttt ccaaaaactt cgtcaaagca aaaatctcaa gggattaatc
      481 atcgatctta gagggaatgg aggcggtttg ctcgatcagg ccataaccat tgtcaatcaa
      541 ttcgtcgata aaggattatt gatcgtaagt acacgcggcc gtaatatcga ccgaaaccga
      601 aattattata cgaccaaccc tcccctcgat ctctctatcc cactggtagt acttgtagat
      661 aaatatactg catcggcttc tgaaatcctc gcaggtgcct tgcaagacct tgacagggca
      721 gtagttatag gagaaaaaac cttcggaaaa gggttggttc aaaacatctt gcctctccca
      781 tacaataatc aattgaagat taccattgcc aaatattaca ttccaagtgg gcgatgtatt
      841 caagccatcg attatttcaa acacgaccaa aatcgaacat ctgtgcaaac caaacctgta
      901 ttctataccc acaacggccg tatggttttc gaacaaggag gaattgaacc cgaccttcct
      961 tctgattcag tatctaacaa tcctcttatc aattcattat ttcagaattt tataattttc
     1021 gattttgtga atgagttcgc ttatcaacat ccgcacatcg atcctttgga aaagtttact
     1081 ctggataaca agatctttaa tgatttcatt caatttacta ccgatcgcta taaagattgg
     1141 gactatgaaa atataaacct gatgcaacaa ttgagaaatc aactttcggc acaaaaccgg
     1201 ttggacaacg aaattaagtc ttcactcgat aaaatcgaac tacagcttta cgaccaaata
     1261 ccaattcttc tttcacaaac acgggaagat ctctcatttc cattgatgag tgaaatcatt
     1321 agcagataca ccaaaccaca ggataggata aaatttttaa ttactaacga tcccgaaatt
     1381 accaaggctg ccaatctgct gcgtgatatg actacatatc aaagtatcct tgccgggact
     1441 catcctaatt gccataaccg tttacgaccg gctaatccat aattccatga caagggaaaa
     1501 actattttat tacatttatt tactcggttt tatgagtctg gtagtggcca tacccacatc
     1561 caattatgta atgagtcttt cccagattat tatgattggt gcatggctat tctatggaca
     1621 ttataaaatc ggcttaaaac aactgctaaa caaaaaaatt gcttgggtat tcatttcatt
     1681 ttaccttctt catatacttg ggctaatcta tacgacagat tttgcttatg ccattaagga
     1741 tttaagaacc aagttgcctt tgttggtatt gccttttgta atatttacca tgcccaggcc
     1801 ttcgaataaa tttttacagc ttatcgtctg gttgcatgca ttggcaattt tcttagccag
     1861 cctcatcggc atttacttgg catggcaaaa aggatatgta aattacaggc tctattctcc
     1921 aggaatttcc catatcaggt ttggattgaa catttgtctt tcggctgtat tgctattata
     1981 cgacatcatc catcgaaaaa aattgattcc gagaatttta gcctccctcc tcattgcatg
     2041 gtatatcgtt tatcttagtc tttttggttc agtaactgcc attgttattc tgggtattct
     2101 tctggtgata ggcatcagta ttttcataat acgtcacagc cctgtatggg gaaaaattat
     2161 ctattccttc attttaaccg cctcaatatt aggtttttca ttcttgataa aggaaacttt
     2221 gcaactcata ctccatcatt caaaagaaaa aatcgacttt gcaaacctcg aaaagttcag
     2281 tgccgatggg catgcttact tacatgatac ggtttacttt gggaaggaat ttgggcagta
     2341 tgtgggttta tattattgcc ccgacgaact tccgcaagcc tgggcaaaac gtagcaaact
     2401 cgattactat gggaaggacc ttgcaggcca acccttgatg gctactatcg taagatatct
     2461 caacagcaaa ggtttacgca aggatgcccg agggctcgcc cagttatcag acgaagaaat
     2521 cggatggata gaaaaaggcg tggccaacgc tcaatatatg caaagcaatg ccctaaagcg
     2581 tacgattatg gaatttgccc ttggatatga acatttcaaa gagggaagtg gcaccagggg
     2641 aaatagtatt atacaaagat tcgaactctg gcgagcttca atcgaaatca tcaaaaaaaa
     2701 tccagtgatc ggagtcggaa ctggcgatgt tccacaagca ttcaaacaac agctcatggc
     2761 tatgcattcc gacttgagcc ataccagtct acgttcgcac aatcaatatt taagcattac
     2821 cattgctttt ggcattattg gtttgctatg gtttctatgg atactactct accctcttaa
     2881 aatcaatcaa ttatacaaag actggcgtta tgtggccttt ttaatcatca tggcagtttc
     2941 tatgatcaac gaagatacta ttgagtcgca agccggaatc acttattatg tatttttttc
     3001 cttattttta tatcttcttt cgatagaaaa ggagaccatg aataattttc cttgaaatcg
     3061 caaaatcatt gtatcatgaa aaccctaaca ctcattacag aaattgagga attcgactcg
     3121 gtagatcaat tgcctgaaaa agataaggag cttctgctcg aggctcgaga ggcagcttcg
     3181 aaagcttatg ctccttactc gaattttaag gtaggagctg ctattcgaat gggaaatggc
     3241 aaaatagtac agggaagcaa tcaggaaaat tcatcttatc ctgttggctg ctgtgccgag
     3301 cgtacagcat tattttatgc ttctagttgc ttttggggtg aaaaaatctg tgcgattgcc
     3361 atagcctctc aaacaacgaa tagtgatggc acacgccctc tcagcccttg cggaatgtgt
     3421 cgccaggcaa tattagaata tgaaattctt cagcaagctc ctattcgtat tctcatgctg
     3481 agcggaagta ccaaagtttg gattgctcca tccgcagtaa gtttgctccc cttccaattc
     3541 gatcatcaat atcttgaaca gtaattgaat attctataat attaaatttc gatgaatcca
     3601 accatttaat tagataagat ataaagataa aaaaataagg acttcaaaat tctcagcgcc
     3661 atctgaactg gtaagaagcc agagcatcga gtaggtaggg agattgctcc cccgccctct
     3721 caccccaccc agcatgctct
//
LOCUS       DAOORU010000190         3709 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_29649_length_3709_cov_5.114395, whole genome shotgun sequence.
ACCESSION   DAOORU010000190 DAOORU010000000
VERSION     DAOORU010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29649_length_3709_cov_5.114395"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="PLF20_09520"
     CDS             <1..1614
                     /locus_tag="PLF20_09520"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HOX81275.1"
                     /translation="TDKIGQQEKAIARADTSFTLKTIDQLEKEAREKINKRYADWFHE
                     MNKLEKADRFADYLNCLVSVFDPHTNYFPPKEKEDFDIQFSGQLEGIGATLQSKDGYV
                     TVVSIVPGGPAWKQGELEVNDKIIKVAQEGSEPVDITDWRVDKAVQLIRGKKGTLVRL
                     TVRKLNGVEKEIAIQRDVVIIEETYAKSAVVEDTLTRGKVGYIFLPSFYANFEDPNGR
                     SSSRDMLQEVEKLKNAGVQGIIIDLRGNGGGSLQDAIEIAGLFIDQGPVVQVKGRVGN
                     PRVYGDPQPGIVYEGPLIIMVNPISASASEILAAALQDYHRAIITGTSKTFGKGTVQR
                     FFNLDDLARGFDEVKPLGSLKLTIQKFYRIDGGSTQLKGVSPDIIMSDPYTYLEIGES
                     ELKYPMEWTQVNPANYSPFNNEYLFTSLEKKSTERIKNDERFQLLDEHARQIKVRRDK
                     TLIPLKMDDFKRQNDENLEQSKAFDKLMKDTLSLKSTPLSVDLQRIGSDTTKLNILKK
                     WTQSLRTDPYLLESVRIVRDWNAAIVQKR"
     gene            1695..3098
                     /locus_tag="PLF20_09525"
     CDS             1695..3098
                     /locus_tag="PLF20_09525"
                     /inference="COORDINATES: protein motif:HMM:NF038133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor L domain-containing protein"
                     /protein_id="HOX81276.1"
                     /translation="MNASAFSKIILFLSLFFTILLAKAQVEISVGHNANELVQMLLGQ
                     GVTISNITGSGLQSATNPNIWQAGKFHGGLPDIGIDSGIILTSGNALLAQGPNNNGNS
                     GYGAGNPGDLMLTNLAGVNTNDACILEFDFTPFYDTIVFRYVFGSEEYHQYVTSYNDI
                     FAFFISGPNPFGGTYDNVNIALIPGTNLAVSMYNINFGNQNGDCPKGTSCVNCEYLID
                     NCESGKGIEYDAYTMVFTAMAIVVPCQTYHLKLAIADAMDSAWDTGVFLEAGSFSSPG
                     VTITPEYSTASGQAVAVEGCSYVDLVVTLPFVQPDTVWLVFDSIRGTATNGVDCNFIS
                     DSIFIAPGELSNFIRIVPIYDGEIEPLENFIFYYSSTSCSGTQVASVRVDIADWNPVN
                     IGSDTTICEGQSITFDAGEGYRSYLWQDGSSDRYFTTNEPGTISVTVYDIYLCPASDS
                     LMLNVATLPQPLIIKHN"
     gene            complement(3140..>3709)
                     /locus_tag="PLF20_09530"
     CDS             complement(3140..>3709)
                     /locus_tag="PLF20_09530"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOX81277.1"
                     /translation="LTINNINRFSFRLSPQSFFQTNRFIAPKLFEYVAHKAGVKSDDT
                     IYDLYCGTGVLGICMANQAKKVVGIEFSEAAIEDAKENARLNGFHNCTFIQGDVLTTL
                     TVDFIKKYGHPDIVIIDPPRSGTLIEIKKILLLESPQKIIYVSCNPQALARDLQMLIL
                     KYEIEEIQPFDMFPQTPHIETVVVLNLKK"
BASE COUNT         1101 a          702 c          786 g         1120 t
ORIGIN      
        1 accgataaaa tcgggcagca agaaaaagca atcgcccggg ccgatacgag ttttactctc
       61 aaaactatcg atcaattgga aaaggaagct agagaaaaaa ttaataaaag atatgcggat
      121 tggtttcacg aaatgaataa actcgaaaaa gccgaccgtt ttgccgatta tttgaattgt
      181 ctagtgagtg ttttcgaccc tcataccaat tatttccccc ctaaagaaaa ggaagatttc
      241 gacatccaat tttctggtca gctcgaaggc attggagcca cactacaatc gaaagatggt
      301 tatgttaccg ttgtcagtat tgtgccgggc ggccctgctt ggaaacaagg cgagcttgag
      361 gttaatgaca aaattatcaa agtggctcaa gaaggaagtg aacccgttga tattacggat
      421 tggcgtgtgg ataaggccgt tcagctcatc cgtggtaaaa aaggtaccct tgtgaggctt
      481 accgtacgaa aacttaatgg agtagaaaaa gaaatagcca ttcaacgaga tgttgtgatc
      541 atagaagaaa cttatgctaa atcggctgtg gttgaagata cacttactag aggtaaagtt
      601 ggttatattt tcttgccttc attttatgct aatttcgagg accctaatgg aaggagctct
      661 tctcgtgata tgcttcaaga agtggaaaag ttaaagaatg ctggcgtaca gggaattatt
      721 atcgacctta ggggcaatgg gggaggttca ctccaggatg ctatcgaaat tgctggcctt
      781 ttcattgacc agggaccagt agttcaggta aaaggccggg ttggtaatcc cagagtatat
      841 ggtgatcctc agcctggcat cgtttatgaa gggcccctca tcattatggt gaatcccatt
      901 agtgcctcag cttcagagat tttagccgca gcactgcaag attatcatcg tgccattatt
      961 acaggaactt ctaagacctt tggaaaaggt acagttcaac gattttttaa ccttgatgat
     1021 ttggcacgcg gtttcgatga agtaaaaccc cttggctccc ttaaacttac tattcaaaaa
     1081 ttctatagaa tagatggtgg ttctactcaa cttaaaggcg tctctcccga catcatcatg
     1141 agcgatccct atacttatct cgaaataggc gaaagtgaac tgaaatatcc catggaatgg
     1201 acacaagtga atcccgctaa ctattctcct ttcaataatg aatatctttt taccagcttg
     1261 gaaaagaaaa gtactgaaag aataaaaaat gatgagcgct ttcaactgct cgatgaacat
     1321 gccagacaaa ttaaagtccg tagagataaa accttaatac cattgaaaat ggacgatttt
     1381 aaacgtcaga acgatgaaaa cctcgaacaa tcaaaggctt tcgataagct gatgaaagat
     1441 accttaagcc tgaaatcaac cccactatct gttgatttgc aacgaattgg ctctgacact
     1501 accaagctga atatcttgaa aaaatggacg caaagcttac gcacagaccc ttatctactc
     1561 gagtcagttc gaattgttcg cgattggaat gcagcaatcg ttcaaaagag ataatttatt
     1621 taatcactat cccttagctt agaagacgaa atttttcagg cctttttata tatttgtatt
     1681 aataatatgt tgatgtgaat gcatctgcct tttctaaaat tattttgttt ctttcactct
     1741 tttttactat tctcctagca aaagcccaag tggagattag tgtgggacat aacgccaacg
     1801 aattggttca gatgctgttg ggacagggag taactatcag caatattacg ggcagtggat
     1861 tgcagtcggc aaccaatcct aatatatggc aggcaggaaa atttcatgga ggtttacccg
     1921 atattggtat cgatagcgga attattctta caagtggaaa tgctctttta gctcagggcc
     1981 ctaataataa tggaaactcc ggctatggtg caggcaaccc tggcgatctc atgcttacta
     2041 accttgccgg agtgaatacc aacgacgcct gcattctcga attcgatttc accccatttt
     2101 acgataccat tgtttttcga tatgtctttg gatcggaaga atatcatcaa tatgtgactt
     2161 catacaacga tatttttgcc tttttcatca gcggtccaaa tcccttcgga gggacttatg
     2221 ataatgtaaa cattgctcta attccaggaa ctaatttagc ggtgagcatg tataacatca
     2281 attttgggaa tcaaaatggt gattgcccca aagggacttc ttgtgtaaat tgcgagtatc
     2341 tgatagataa ttgcgaatcg ggtaaaggta tagaatatga tgcctatacc atggttttta
     2401 cagcaatggc tattgttgtc ccctgccaaa cctatcatct caagttggct attgccgatg
     2461 caatggatag cgcatgggat acaggtgttt ttcttgaagc tggtagtttt agcagtccag
     2521 gtgttaccat tactcctgaa tattcaactg cttctgggca ggcagtggca gtagaaggct
     2581 gctcctatgt ggatttagtg gttactctac cttttgtgca gccagatacg gtttggctgg
     2641 ttttcgatag cataaggggc actgctacta acggtgttga ttgcaacttt attagcgata
     2701 gcatctttat tgcaccaggt gaactttcta actttattcg tatcgttccc atttatgatg
     2761 gagaaattga acccctcgag aatttcattt tctattattc ttccacctct tgttcaggaa
     2821 ctcaggtggc ttcggtgcgg gtggatattg ctgattggaa tcctgtgaat attggaagtg
     2881 atactacaat ttgtgaaggc cagtctatta ccttcgatgc aggtgaaggc tatcgttctt
     2941 atttatggca ggatggaagt agtgataggt atttcacaac aaatgaacca ggtaccatct
     3001 cagttaccgt gtatgatata tatctgtgtc cagcctcaga ttcattaatg ctcaatgttg
     3061 caacattgcc acaaccatta ataatcaaac ataattgaga ttagaatttg gatgaattga
     3121 tttcaaaaga gaagaattct tattttttca gattaagaac gacaacagtt tctatatgag
     3181 gggtttgtgg gaacatgtcg aaaggttgaa tttcttcgat ttcgtatttt aatataagca
     3241 tttggaggtc gcgagctaga gcctgaggat tacagcttac atatatgatt ttttggggag
     3301 attccaataa aagaatcttt tttatttcga taagggtgcc cgaccgaggg gggtcgatga
     3361 tcacaatatc gggatgccca tattttttaa taaaatcaac ggtcaaggtg gtcagtacat
     3421 ctccttgaat gaaagtgcag ttatgaaatc cattcagacg tgcattttct ttggcgtctt
     3481 ctatagccgc ctcagaaaat tcgatcccta cgactttttt tgcctgattt gccatacaaa
     3541 ttcctaaaac acctgttcca caatataagt cataaattgt atcatcagat tttactccag
     3601 ctttgtgagc tacatattca aacagcttgg gagctataaa acgattggtt tggaaaaaac
     3661 tttgagggct cagcctaaag ctgaatctat ttatattatt tattgtaag
//
LOCUS       DAOORU010000191         3675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_30001_length_3675_cov_4.516575, whole genome shotgun sequence.
ACCESSION   DAOORU010000191 DAOORU010000000
VERSION     DAOORU010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3675
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30001_length_3675_cov_4.516575"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="PLF20_09535"
     CDS             complement(<1..198)
                     /locus_tag="PLF20_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81278.1"
                     /translation="MEFFEKLPPLEHPYLRAIAARYMPEEYKEIIKNVFLKDPDQNVR
                     LVAANRLRWSEDKEAIQELIKA"
     gene            complement(185..880)
                     /locus_tag="PLF20_09540"
     CDS             complement(185..880)
                     /locus_tag="PLF20_09540"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOX81279.1"
                     /translation="MNLKRRMKAIPGIFTMILMMKGGIAFSSSSSACMFMPPVDSNKI
                     VLKDTLHFADSLEDTDSEEIDDLIASNWIVNQIFAYRDVKEIPLNICIQLVDSLHSFI
                     LPVEGKVWNGFGPRGTRIHKGVDIGLNHGDPVKTAFDGVVRHAARNRHGYGNIVVIRH
                     FNGLETYYAHLSRINVKPGDQVKAGDIIGLGGSTGRSRGSHLHFEVRYFDKAFFSMCS
                     FAAAQKSRDYGIF"
     gene            complement(1206..1976)
                     /locus_tag="PLF20_09545"
     CDS             complement(1206..1976)
                     /locus_tag="PLF20_09545"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOX81280.1"
                     /translation="MENAVCQLSAAPLRVEPLHKAEMISQLLFGEPVEIIERRPPWCL
                     IKSKIDYYEGWVDAQQLEIMPQTPQFNEDNLLICHQLLSSLIHCITGEKQLITFGTML
                     TAENGKVKVGSSEYYKHDDFMPFFRKTTSNDIINSAKLWLNTPYLWGGKTPLGVDCSG
                     FVQLVFRCNGQKLPRDAYQQAELGIDIDFVSESMPGDLAFFSNDESHISHVGILIDNS
                     RIIHASGKVRIDAFDHQGIYNLSLKTYTHQLRLMKRLL"
     gene            complement(2032..>3675)
                     /locus_tag="PLF20_09550"
     CDS             complement(2032..>3675)
                     /locus_tag="PLF20_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX81281.1"
                     /translation="CHYKLRFEFFTMRRHHPILIIFLLLSSYGMAQIKNSKLFPHSEE
                     PGNLPTLCLHPPSELLHRSESPKSALPQIIGKVYDTTFDVMKLARYDENGEGRIYDLR
                     IHIDGVEALILYFSALYLPQSCFLYTTGRDVDNIYTFSFVDNPQGGSYSIPMIDGEYI
                     ILRVFVPYDCSAEPHIIISGVGIIPKGEKGYGGSGSCEVNVNCNEGLQWKNQKNATVR
                     ILIRNVNSIYWCSGTLMNNGRKDRTPYILTANHCGKGATKENLKQWQFFFNYEAANCS
                     QPALEPELKPSVGGTKKAASDVSISNLGSDFYLLTLNQGIPENVNAFFSGWSRLDYPS
                     ASGVCIHHPQGDIKKISTYTTPTLSSSWENMSNSHWKVSWSATENGHGVTEGGSSGSA
                     LFNSNGLLMGTLTGGQSSCDSSNLNQPDYFGKFSAHWDLNGQADTLRLQPFLDPDHIG
                     MTTLEGMTLGLDQTFDILIFSVFPNPSSQNISLHLPDSFSTLKIMVEIFHPTGQKVMK
                     IELDGIHPVPIQISHLCPGIYFIRITSDEKSSIAKFIKQ"
BASE COUNT         1080 a          740 c          714 g         1141 t
ORIGIN      
        1 ggctttgatc aattcctgta tcgcttcttt atcttcagac catctcaggc ggtttgccgc
       61 tacaaggcga acattctggt cagggtcttt caagaagaca ttttttataa tttctttgta
      121 ttcttcaggc atataacggg cagcaatggc cctgagatag ggatgttcca ggggaggcaa
      181 tttttcaaaa aattccatag tctctagatt tttgagctgc tgcaaaagaa cacattgaga
      241 aaaaagcctt gtcgaaatat cggacttcga aatgcagatg agagccacgg cttcggcccg
      301 tactgcctcc taaacctatt atatctcccg cttttacctg atcgccaggt tttacattga
      361 tcctggaaag atgggcataa tatgtttcta atccattaaa atgcctgatg actactatat
      421 tgccatatcc atgcctattt cttgcagcat gcctcactac tccgtcgaaa gccgtcttta
      481 cagggtctcc atggttcaat ccaatatcta cacccttatg tatccttgtt cctctgggac
      541 caaatccatt ccatactttt ccttctaccg gtaaaataaa tgaatgcagt gaatcgacca
      601 attgaataca aatattgagc ggaatttctt ttacatctcg ataggcaaaa atttgattca
      661 caatccaatt gcttgcaatc aaatcatcaa tttcttctga atcggtatct tcgagtgaat
      721 cagcaaaatg taaagtatct ttcaacacaa tcttgttaga atctacaggc ggcataaaca
      781 tacaggccga agaactcgac gaaaatgcaa tccctccttt catcatcaag atcattgtaa
      841 atattccggg gatagctttc attcttcttt taaggttcat cagtcagtat ttttttattc
      901 gagtgatttt ttagtatgtg atcgttttat aaaaattttt cgcaaagtac gaattttttt
      961 aaaatccaaa acatttcaaa atattttagc cttagaatat atctttcgga attgttgata
     1021 accgaaattt ggtaaatttt cgagaggcgg ctgtactgtt ctcgttttac aacgatatcg
     1081 aatttaacaa tagatttaaa tgtaaaatgg atgttctggc tcatttcaat ggcataaagc
     1141 gtatgaatgg ataaatttcc caaatgagga aaatcgatgt tgttaagcga atgagcatca
     1201 aaattttata aaagtcgttt catcaatctc aattgatgtg tataggtttt taatgataag
     1261 ttatagattc cctgatgatc gaaagcatct attcgaactt tgcccgaggc atgaataatt
     1321 cgactgttgt cgataagaat ccccacatgc gaaatatggc tttcgtcatt ggagaaaaag
     1381 gctaagtcgc caggcatgga ttcggaaacg aaatctatat caatacccaa ttcggcctgt
     1441 tgataagcat cgcgaggtaa tttttgtcca ttgcatcgga aaaccagttg aacgaaacct
     1501 gagcaatcta ctcctaaagg tgtttttcca ccccataagt agggggtatt cagccataat
     1561 tttgccgaat ttataatatc gttgctcgtc gtttttctaa aaaaaggcat gaaatcgtca
     1621 tgtttatagt attctgaact ccccacttta accttgccat tttctgcggt aagcattgtt
     1681 ccaaaagtga tgagttgttt ttcacctgtt atgcaatgaa tgagagaaga aagcaattga
     1741 tggcatatta acagattatc ttcgttaaat tgtggagttt ggggcataat ttccaattgt
     1801 tgtgcatcta cccatccttc gtaataatca attttacttt ttatcaaaca ccatggcgga
     1861 cgtctttcga ttatttcaac cggttcgcca aagagcaact gcgaaatcat ctcggcttta
     1921 tgcaaaggct ctaccctcag gggtgcagca ctaagctggc aaactgcatt ttccatcttc
     1981 aaaaaacaat gtcatagttg aaacatacat gtgaataagc gtaaattttg gctactgttt
     2041 gataaatttt gcaatcgaac ttttttcgtc cgaagtaata cggataaaat agatacccgg
     2101 gcataaatgg ctaatctgaa taggaacggg atgaattcca tccaattcta ttttcattac
     2161 cttttgacct gtaggatgaa aaatttccac cattattttc aaggtcgaaa agctgtcggg
     2221 aagatgtaaa gagatattct gactggaagg attgggaaag acagagaaaa ttaggatatc
     2281 gaaggtttgg tctaatccta aggtcattcc ttcgagggtg gtcattccaa tatgatcggg
     2341 atcgagaaag ggttggagtc gcaaagtatc ggcttggcca ttcaaatccc agtgtgcaga
     2401 gaattttccg aaataatcgg gttgattgag gttggaggag tcgcaagaag actggcctcc
     2461 ggttaaagta cccattaata ggccattact gttgaacaag gcgcttcccg aagagcctcc
     2521 ttcggttacg ccatgaccat tttcggttgc cgaccagctc accttccaat gagaattact
     2581 catgttttcc cagctcgatg atagggtagg tgtggtatag gttgatattt ttttgatgtc
     2641 gccttgcgga tggtgaatgc aaacccctga tgcggaaggg taatccaacc ttgaccaacc
     2701 agaaaagaag gcattcacat tttcagggat accctgattg agagtcaata aataaaaatc
     2761 gctccccaaa tttgaaatgc tcacatcgga agccgccttt ttcgttcctc ccacagaagg
     2821 tttcaactct ggctctaatg caggttgcga acaatttgca gcctcgtagt taaagaaaaa
     2881 ctgccattgt ttcagatttt ctttcgtggc tcctttgccg caatgatttg ccgttaaaat
     2941 ataaggtgtt ctatccttgc gcccattatt catcaatgtc ccgctacacc agtaaatcga
     3001 gtttacattt cttatcagta tccttacggt agcattcttt tgatttttcc attgtaaacc
     3061 ttcgttacag tttacattta cttcgcagct tcccgaacca ccataccctt tttcgccctt
     3121 gggtatgatt ccaaccccag atatgataat atgaggttcg gcactacagt cataaggcac
     3181 aaaaactctg agtattatgt attctccatc aatcatcgga atgctgtagc taccgccctg
     3241 tgggttgtca acgaatgaaa aggtataaat attatcgaca tccctaccgg tagtatataa
     3301 aaaacaactt tgcggtagat ataaagcgga gaaatacaaa ataagggcct ctactccgtc
     3361 gatatggatc cttaaatcat aaattctccc ttctccattt tcgtcatacc gagccagttt
     3421 catcacatcg aaagtggtat cataaacttt tccaatgatc tgaggcaaag ccgattttgg
     3481 actttcactt ctatgcaata gttcgctggg agggtgtaag catagcgtag ggaggtttcc
     3541 cggttcttct gaatgaggaa acagtttgga attctttatt tgagccattc cataagagga
     3601 gagaagtaaa aagattatta aaattggatg atgcctgcgc atggtaaaaa attcgaatcg
     3661 aagtttgtaa tggca
//
LOCUS       DAOORU010000192         3616 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_30663_length_3616_cov_5.097725, whole genome shotgun sequence.
ACCESSION   DAOORU010000192 DAOORU010000000
VERSION     DAOORU010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30663_length_3616_cov_5.097725"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PLF20_09555"
     CDS             <1..279
                     /locus_tag="PLF20_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOX81282.1"
                     /translation="PMQIKIDFLCRDSILAAPIALDLVLLMNLAQRVGFYGIQEWLSF
                     YFKSPLHSPDVVPEHDIFIQHIKLKNTMREIMDEEPITHLDEKIKQFK"
     gene            complement(374..931)
                     /locus_tag="PLF20_09560"
     CDS             complement(374..931)
                     /locus_tag="PLF20_09560"
                     /inference="COORDINATES: protein motif:HMM:NF027443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4833 domain-containing protein"
                     /protein_id="HOX81283.1"
                     /translation="MTSNLWANSRFSRSFGVRSVFSLVFIHLCVFVSLGGDLPTVNRV
                     FVIERSKNANQVCYDLNIDSRGNLNADKPLKVYWLNRTDRPGAMDNLSLIQAKYAYGY
                     SSKSLGNGDYEITLVAFPERKLRLEKGSDGRYRAIISIAGVRAQLTRIFVQSEPGNYT
                     KVAWVDLYGISLNSGKEVSERIIAR"
     gene            1127..>3616
                     /locus_tag="PLF20_09565"
     CDS             1127..>3616
                     /locus_tag="PLF20_09565"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HOX81284.1"
                     /translation="MNKSIDEKWHSLSYFEEIRLIISHPFHTAKYIFTQIKHGHIPNI
                     IQFTNKRTKLFYSWVVCSLLMMFSWGITIVQAQPHQNTTIIQGKITDANTNEPIPFAN
                     VFFKGTTIGTTTNFDGRYRLETTTPGDSLTVTVISYKSKSKKIQKGKNQTLDFQLEPT
                     SFELEEVVVRAGENPAYPIIRKARENRKFFNPENLDAYEYKSYVKVDISLDQINENFK
                     NSKTFKPYSALMDSLQKAAGEEGKLVIPFFVSEQINEVYYRKNPKRNKTVVKASRMNG
                     LIIDNYQIFESYLGKTLQEYNFYNNFQEILDRSFITPLATGSFAYYDYFIMDTVMVDN
                     DSCYELKVKPRRPLDLVFSGKIWIDTKNYALRRLDLEITSSVNINFIDKYKIQQDYER
                     QSTGHYLPTKTRVLVDLQQTSDSAVGFIGKYYIANKDYIINQPRPPSFYDRNVEVDMA
                     AKTYSDDFWNEQRKEMIADHEQVKSSLEAIDSLKKAPQITFIRRALRIIWDGYYNFGK
                     IELGHWYTMFGNNPVEGFRFQTSVRTNINWNYYWILSGYGAYGFKDETFKYRLQVERF
                     LNRSKWEKIGLRYSYDMMRLGVDPEFLESHVFLNYIFLFSSQFGYLHRMSLSQDARIW
                     YETDHWRGWNTKLMIRTKTFHSQGDYYFGYYDDNDLIRTKYRTSEFHLITSYSKRRTW
                     LVENNWRVGAEALKSQLWTFHATLGFKNVLGSDFTFQKFSLNVMHKWRTGYFGQLDYS
                     LTGEVTLGKVPYPEAIIPQGNEPVFAAEKAFNMMNFFEFVADKSISGILLHHFDGLFF
                     NRIPLLKHLKWREVAGFNFVFSTYGKKNYIRT"
BASE COUNT         1164 a          797 c          609 g         1046 t
ORIGIN      
        1 cccatgcaaa ttaagatcga cttcctttgc cgtgattcca tacttgctgc tcctattgct
       61 ctcgatctcg tgttgttgat gaaccttgct caaagagttg gcttctacgg aatacaagaa
      121 tggctttctt tctacttcaa gagcccattg cattctcccg atgtggttcc cgaacatgat
      181 atctttatcc agcatatcaa gctcaagaat accatgcgag aaattatgga cgaagaaccc
      241 atcacccatc tcgatgaaaa gataaaacaa ttcaaataaa tttcatagaa gaatttttta
      301 atattggggc tcaggcttta cctgagccct atttccttat tcacaaaaca tcctcactat
      361 aaggcaaaat cttctaacgg gcaataattc gctcacttac ctccttacca ctgttcaaag
      421 aaattccata caaatcgacc caagctacct tggtataatt tccaggttca gactgtacaa
      481 aaattctggt taattgcgcc cgaaccccag ctatacttat aattgctcga tatctaccat
      541 cgctaccttt ctcaagtcta agcttgcgct ccggaaatgc cactaaagtg atttcgtaat
      601 ccccattacc taatgacttt gatgaatatc cataagcata tttcgcttga atcaacgaca
      661 gattatccat agctccaggc ctgtctgtcc tatttaacca ataaactttt aacggtttgt
      721 cggcattcaa atttcctcga ctgtcaatat tcaaatcata acaaacctga ttggcatttt
      781 tcgagcgttc gatcacaaaa actcggttaa cagtaggtaa atccccacct aaagaaacaa
      841 aaacacataa atgaatgaat actaagctaa acacactacg aacaccaaag cttctgctga
      901 atcgactgtt tgcccataaa ttcgatgtca tcaattccat actgcaaatt tatcttttcc
      961 ctttatttat attaagtata catttattta tagtaatttt gccggttcaa aaatctacat
     1021 tagcttgata actttttaac taaccagttg tcaaatatac tctgattatt ttcgttttaa
     1081 atttttatcg ataatcaggc aacaaagatt gaagtcgaac ttttttatga ataaatcaat
     1141 agatgaaaag tggcattcat tatcttattt cgaagagatt cggttaatta taagccatcc
     1201 ttttcatacg gcaaaataca tttttactca aataaaacat gggcatattc caaacattat
     1261 acaatttacg aataagagaa ccaagttatt ttattcgtgg gtggtttgtt cactcctaat
     1321 gatgttttca tggggtatta cgatcgtaca agcccaacct caccaaaata ctactataat
     1381 tcagggcaag attaccgatg ccaataccaa cgaacccata ccctttgcta atgtcttctt
     1441 caaaggaact actataggta cgactaccaa ttttgatggc agatacaggc tcgaaaccac
     1501 tactcctggc gattctctca ctgtaacggt tatcagttac aaatccaaat cgaaaaagat
     1561 acagaagggt aaaaaccaaa ccttggattt tcaactcgaa cccacttctt ttgaattgga
     1621 ggaagtggta gtaagggcag gagaaaatcc agcttaccct attatcagaa aagcacgtga
     1681 aaatagaaaa ttcttcaatc ctgaaaattt agatgcatac gaatataaga gctacgtaaa
     1741 agttgacatc agtctcgacc aaatcaatga aaatttcaag aatagcaaaa ccttcaagcc
     1801 ttattccgct ttgatggaca gtttacaaaa agccgctggc gaggaaggaa aattggttat
     1861 accctttttc gtttctgaac agatcaatga ggtatattac aggaaaaatc ccaaacgaaa
     1921 caaaaccgtt gtgaaagcaa gtcgtatgaa tggcctgatt atcgataact accagatttt
     1981 tgaatcttat cttggaaaaa cccttcagga atataatttt tacaataact ttcaggaaat
     2041 tctcgaccgc tcgtttataa cccctttagc tacaggaagt tttgcttact atgattattt
     2101 tatcatggat accgtcatgg ttgataacga cagttgttac gaactgaaag ttaaaccccg
     2161 tcgacccctt gatctggttt tttcagggaa aatatggatc gatacgaaaa attacgcctt
     2221 acgccgcctc gatctggaaa ttaccagctc cgtgaatatc aacttcatcg ataaatacaa
     2281 aattcaacaa gattatgaac gacaatctac aggccactat ctgcctacca aaacaagagt
     2341 tctggtcgat ctgcaacaaa ctagcgattc agctgtaggc ttcatcggta aatactatat
     2401 tgccaacaaa gattatatca tcaatcaacc ccgacctcct tcattttacg atagaaacgt
     2461 tgaagtggat atggcagcca agacgtattc cgacgacttt tggaacgagc agcgcaaaga
     2521 aatgatagct gatcatgaac aagtaaaaag ttcgctcgag gcaatcgatt cactcaaaaa
     2581 agcccctcaa attacattta ttcgaagagc tcttcgaatc atctgggatg gctactacaa
     2641 ctttggaaag atagaacttg ggcactggta caccatgttt ggcaataacc ccgtcgaagg
     2701 gtttcgattt caaacttcag taaggaccaa tatcaactgg aactactact ggatactttc
     2761 aggctatggc gcttatggat tcaaagacga aactttcaaa taccgtttgc aggtagaacg
     2821 attcttaaac cgcagcaaat gggaaaagat aggactacgt tattcgtatg atatgatgcg
     2881 cctgggagtc gacccggagt ttctcgaatc ccatgtattc ctcaattata tcttcctctt
     2941 ttcttctcag tttggatatc ttcacaggat gagcctctcg caagacgccc gtatctggta
     3001 cgaaaccgac cactggcgag gctggaatac aaaattgatg ataagaacca aaaccttcca
     3061 ttctcaaggt gattactatt ttggctatta cgacgataat gacctcattc ggacaaaata
     3121 ccgtacttcc gaatttcatc ttattaccag ctattcgaaa agaagaacat ggctggtgga
     3181 aaacaactgg cgcgttggtg ccgaagcact taaatcacaa ttatggactt tccatgctac
     3241 attgggattt aaaaatgttc tcggcagtga cttcactttc cagaaatttt ccctaaatgt
     3301 catgcataaa tggcgaactg gttatttcgg ccaactcgac tactcattaa ccggagaagt
     3361 tactctggga aaagttccct atcccgaagc cattatccct caaggcaatg aacccgtatt
     3421 cgctgctgag aaagccttca acatgatgaa ttttttcgaa ttcgttgccg ataaatccat
     3481 cagtggaatt ttactccacc acttcgatgg cctgttcttc aaccgtattc cgctgctcaa
     3541 acacctcaaa tggagagaag tggcaggatt caatttcgtc ttctcaacct atggaaagaa
     3601 aaattatata cgtaca
//
LOCUS       DAOORU010000193         3607 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_30761_length_3607_cov_4.681025, whole genome shotgun sequence.
ACCESSION   DAOORU010000193 DAOORU010000000
VERSION     DAOORU010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3607
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30761_length_3607_cov_4.681025"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3362
                     /gene="glgP"
                     /locus_tag="PLF20_09570"
     CDS             <1..3362
                     /gene="glgP"
                     /locus_tag="PLF20_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HOX81285.1"
                     /translation="LGQDIRDDAFMVVTSGRYEFRNKGLDAFIDALGYLDKNYRGDRQ
                     IIAWLMVPFTNKGPNPVLKNRLEEKEPIVPTENEFLTHNLYEPEQDQILSAIKKNQLT
                     NSAKSRIKVIFVPAYLDNQDGIFNKSYYDLLLGCDLSVFPSYYEPWGYTPLESIAYHV
                     PTVTTSLAGFGQWVLQNYDSPNDMVRVLTRNEDNYEEFCEAIAATIKEYSQKSDEELH
                     QIGVRANAMARDFVWENMIDQYWIAYSAAIEKAMARSDLFRPRMAVGHPVAIAEKAEA
                     SWRKIMVDPGVPPRLEGLVRLSKNLWWSWNNNAIELFSMIDKQLWEDCEQNPIALIES
                     LPLDALKKLENDTEFLTKLDTIVAQFDQYMAQAVNKDPKVVAYFSMEYGIHDSLKIYS
                     GGLGILAGDYLKEASDCNKNIIAIGLLYRYGYFQQEISIHGDQIAKSSPQKFSHMPLI
                     PVRDADGNWIKVSIAFPGRTMYAKAWLVEVGRIPLYLLDTEIDENLEIDRSVTHFLYG
                     GDWENRLKQELLLGVGGIRLLHELNIRPHVYHLNEGHAAFIGIERLRDLVQNEKLTFN
                     QAIEIVRSTSLFTTHTPVPAGHDTFTEDLLRAYIPHYADRLTISWEEFIRLGRINPND
                     TQENFSVSVLAINLCQEVNGVSRIHETVTQKMFVKMFEGYFPEELHIGHVTNGVHFPT
                     WASIDWQRIYAHTFGKDYILNQSNPKYWEKIYEVSDENIWAERMHQKEELIKFVKKHV
                     NDDMTRRQENPKLIFRVLESIKVEPLTIGFARRFAYYKRAYLLFFNMERLAKIVNNPR
                     YPVQFIFAGKAHPADQQGQDLIKKIYTVSKQPEFLGKIIFLENYGMEMAKSLIRGVDV
                     WLNTPMRLMEASGTSGEKAVMNGVVNFSVQDGWWADGYKPGAGWAIKEKPTYTNDQFQ
                     DELDAETIYSTLEEEIIPTYYDTDWNGVSSRWVAHVKNTIANIAPHFTMKRQLDDYYK
                     KFYTKLFDRGELMKQNDFELAKNISAWKRRVTRLWKDILVLNMKFPGSEHPALRQGET
                     FHAEVTLDIGELSPEDIGLELIFGRKINGTVDKLLFKNEMNQEIIDYHIVKFFCHIPI
                     TKAGVYDFSFRLYPKHPLLPHRQDFCLVRYF"
BASE COUNT         1133 a          724 c          763 g          987 t
ORIGIN      
        1 ttttaggcca ggacataagg gatgatgctt tcatggtggt tacgagtggc cgctacgaat
       61 ttcgtaacaa aggcctcgat gctttcatcg atgccttggg ttatctcgat aaaaactatc
      121 gaggcgaccg acaaatcatt gcatggctta tggtaccctt taccaataaa ggacccaatc
      181 ctgtcttgaa aaaccgtctg gaagaaaaag aacctatcgt ccctaccgaa aacgagttcc
      241 ttactcataa cctctacgaa cccgaacaag accaaattct atcagcaata aagaaaaatc
      301 agcttacaaa tagcgccaaa agccgcatca aagtgatttt tgtacctgcc tacctcgata
      361 atcaagatgg cattttcaat aaatcttatt atgatctttt attaggatgt gatttatcgg
      421 ttttcccttc ctactatgaa ccatggggct acacccctct ggaaagcatt gcttatcatg
      481 ttccaactgt gactacatca ttagcaggct ttggtcaatg ggttttacag aattacgatt
      541 caccgaacga tatggtaagg gtgctaaccc gaaacgaaga taactacgag gagttttgtg
      601 aggcaattgc ggctacgatt aaagaatatt ctcaaaagtc agacgaagaa ttacatcaaa
      661 taggagtaag agccaatgcc atggcgcgag attttgtttg ggaaaacatg atagaccaat
      721 attggatagc ctattcggca gcaatcgaaa aagccatggc gcgcagcgat ttatttcgtc
      781 ctcgcatggc tgttggacat ccggttgcga tagccgaaaa agccgaggct tcatggagaa
      841 agattatggt agatccgggt gttccaccac gattggaggg cctggtacgt ttgtcgaaaa
      901 atctgtggtg gtcatggaat aacaatgcca tagaattgtt ctcgatgata gataaacaat
      961 tatgggaaga ttgcgaacag aacccaattg ctcttatcga gtcgttgcct cttgatgcat
     1021 tgaaaaaatt ggagaacgat acggaatttt taacgaaact cgataccatt gttgcccaat
     1081 ttgatcaata catggcccaa gctgtcaaca aagatccaaa ggttgtggcc tatttcagta
     1141 tggagtatgg gattcacgac agtttgaaaa tttattctgg aggtttaggc atattagcag
     1201 gtgattatct gaaagaggcc agcgattgca ataagaatat catagccatt gggcttttat
     1261 acaggtatgg ctatttccaa caggaaatct ctattcatgg ggatcagatt gccaagagtt
     1321 ctcctcaaaa gttttcgcac atgccgctga tacctgttcg cgatgccgat ggtaattgga
     1381 taaaggtaag tatagcattt ccgggtcgta ccatgtatgc aaaagcgtgg ttggtggaag
     1441 ttggacgaat ccccctgtat ctgctcgata ccgaaattga cgaaaatctc gagatcgacc
     1501 gaagtgttac acattttctt tatggaggcg attgggaaaa ccgtctgaaa caagaattat
     1561 tacttggggt gggcggaatt cgccttttac atgaacttaa tattcgacct catgtatatc
     1621 acctaaacga aggccatgct gcattcatcg gaatcgaacg gctgagagat ctggttcaga
     1681 acgaaaaatt gacattcaat caggcaatcg aaatagtaag atcgaccagc ctctttacta
     1741 cccatacccc tgttcccgcc ggccatgata cattcactga agacttgcta agagcctata
     1801 ttccacatta tgccgacagg ctaaccattt catgggagga atttatacgg cttgggcgaa
     1861 tcaatcctaa tgatacccag gaaaatttct cggttagtgt tctggctatc aatctttgcc
     1921 aggaagtgaa cggagtaagc cgaatccatg aaacggttac ccagaaaatg ttcgtaaaaa
     1981 tgttcgaagg ttatttccct gaagaacttc atattggcca tgttaccaat ggagttcatt
     2041 tccctacctg ggcttctatc gattggcaaa gaatatatgc acatactttt ggaaaggact
     2101 atatactgaa tcaatcgaat cccaaatact gggaaaaaat ttatgaagta agtgatgaga
     2161 atatatgggc tgaaagaatg catcagaaag aagaactgat aaaattcgtt aagaaacatg
     2221 taaacgatga catgacccgc cggcaggaaa atcctaagtt gattttcagg gttcttgaat
     2281 ccattaaggt agaacctctc accattggct ttgcccgtag atttgcttat tacaaaaggg
     2341 cttacctgtt gttcttcaat atggaaagat tggccaaaat cgtgaataat ccacgttatc
     2401 ccgtacaatt catctttgca gggaaagcac atcccgctga ccaacagggt caagacctca
     2461 tcaaaaagat ttatacggtg agcaaacaac ctgaatttct tgggaaaatc attttcctcg
     2521 aaaactacgg aatggaaatg gccaaaagtt tgatcagagg agtagatgtg tggttgaata
     2581 cacccatgcg attgatggag gcttcgggta ctagtggtga aaaagcagtc atgaatggag
     2641 tagtcaattt cagcgttcag gatggctggt gggccgatgg ctacaaacca ggcgcaggtt
     2701 gggctattaa agagaaaccc acctatacca atgatcaatt tcaggatgaa ctggatgccg
     2761 aaaccattta ttctaccctc gaagaagaaa tcatacctac gtattatgat accgactgga
     2821 acggagtttc atcacgatgg gtagcccatg tcaaaaatac cattgcgaat attgctcctc
     2881 atttcaccat gaaaaggcaa ttggatgatt actacaaaaa attttataca aagctcttcg
     2941 atagaggtga actgatgaaa caaaatgatt tcgaattggc taaaaatata tcggcctgga
     3001 aacggcgcgt aactcgcctg tggaaagata tcctggtttt aaacatgaaa ttccctggct
     3061 cagaacatcc tgccctgcgt cagggtgaaa cttttcatgc agaagttacc ctcgatattg
     3121 gcgaactttc acctgaagat attggtcttg agttgatttt tggacgcaaa atcaatggca
     3181 cagtggataa acttctgttt aaaaacgaaa tgaatcaaga aatcatcgat taccatatcg
     3241 taaaattctt ctgccatatt cctattacta aagccggagt gtatgatttc tcattccgtt
     3301 tgtacccaaa acatccttta ctgcctcatc gtcaagattt ttgtctggtc agatattttt
     3361 aaaaaaggga atttgatcga aaaaatcaga actcctgacc cttcaattgc ctatctatat
     3421 tttacaaaaa tactatctta gaaatgccct caataatccc ttcaaatcct tagcgcactt
     3481 cggtaatttt tttgtcgaga cgccaaagac ctatagggtt ttgtccaaac ctaacaagtc
     3541 tccaagacag ttagatttta acgtcaagaa aagaaattta aaaaaagggt tagaagttgc
     3601 agatgat
//
LOCUS       DAOORU010000194         3598 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_30859_length_3598_cov_5.209145, whole genome shotgun sequence.
ACCESSION   DAOORU010000194 DAOORU010000000
VERSION     DAOORU010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30859_length_3598_cov_5.209145"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLF20_09575"
     CDS             complement(<1..234)
                     /locus_tag="PLF20_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004348852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOX81286.1"
                     /translation="MITKRIIECVPNFSEGRDLGIIRQITDEIEQVEGVKLLDVDPGK
                     ATNRTVVTFVGEPEAVVEAAFRAIKKAASLIDMR"
     gene            375..2315
                     /locus_tag="PLF20_09580"
     CDS             375..2315
                     /locus_tag="PLF20_09580"
                     /inference="COORDINATES: protein motif:HMM:NF019556.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HOX81287.1"
                     /translation="MKIQNSFLTDLLFAFPLMISIPLLGQNHHLQQVNFTSVKITDEF
                     WAPRIKTNHQVTIPISFVKSEETGRIKNFKVAAKLEPGGFCSTYPYDDSDVFKIIEGA
                     SYSLQLFPDPQLEAKLDTLINYIALAQEPDGYLYTNRTIDSLHTHPWVGKKRWENDPK
                     HSHELYNVGHLYEAAVAHYLATGKTTLLNVAIKNADLVAKDFLDKRLPYYPGHQEIEL
                     ALVKLYKLTGNSRYLDLAKYFLDIRKGGNEYNQAHLPVNQQTEIVGHAVRATYMYSAM
                     ADVATLKNVPEYQAAIHRMWNDLINGKYYITGGIGSRASNEGFGNAYELPNEEAYCET
                     CASIGNVLWNYRMFLMDADAKYYDVLERTLYNALLSGVSLSGDRFFYSNPLESHGQHE
                     RQAWFDCACCPSNVCRFLPSLPGYIYALDSSTLYVNLFIQNQATMPVFNHQTGIEILT
                     NYPWDGKVQIRILDSNPVPYAIAVRIPGWARGEAVPSNLYKFADDNISKWKLRVNNKE
                     YPFELSKGYVFINKEWNRGDEIELELPMEVRMVKAHSTVKADSGKIALQRGPLVYCLE
                     WADQPGKDLWNIALNLQKGFEIIRDDSLLPNCINIKGEACQIEKFASDKCFEFNAIPY
                     FLWAHRGKGPMRVWIPYFPSRH"
     gene            complement(2360..3289)
                     /locus_tag="PLF20_09585"
     CDS             complement(2360..3289)
                     /locus_tag="PLF20_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81288.1"
                     /translation="MDKPELNKIEIQKVSIQRYEQDLFRIPIDSLQELLKHLANKYPL
                     FIGNEYDNPASLLQMRQYLNDPQIIEAYVQACKTFPSLAQIEDGLTEAFRYIHYYFPS
                     WHPPKSVYSYISGYDLAEGIFIDDSVLIIPLDNYLGSNFPGYRTIGIPKYIAERMTPD
                     YLIPDIIKTIAQQFLPPFPVDGALVDQMIAYGQLLFFAQNILPKTPPYFLMGYTAEKY
                     KWCQDNEKEIWSFLLNQDLLFSKDKSIIMKFIQESPTIQGFPKGSPGRIGQYMGLRII
                     ESYARKHPDKNLKEIIQEEDYAVLFNQSGYKPR"
BASE COUNT         1074 a          723 c          706 g         1095 t
ORIGIN      
        1 gcgcatatcg atcaatgaag ccgctttttt gattgctctg aaggcagctt cgacaaccgc
       61 ttcgggttcc ccaacaaatg taactaccgt acgattggta gccttgcctg gatcaacatc
      121 cagcagcttg acaccttcta cctgttcaat ctcgtccgta atttgcctga tgatccccaa
      181 atctcttcct tcactgaaat tgggaacgca ttcaatgatt cttttcgtta tcatgaagaa
      241 tttttttgca aagttatggc ctttcgattg tctgcccaaa gtcttcccgt aatgaaatac
      301 gtatgggaaa ttcgaatggc ctttctgatt ttggatgaaa tgcgtaattt taagcaatta
      361 aaattaactg cacgatgaag atacaaaaca gctttctgac agatttacta tttgctttcc
      421 cattaatgat ttctattccc ttgttggggc aaaatcatca tttgcaacag gtaaatttta
      481 cttcagtaaa gattaccgat gagttttggg caccacgcat caaaactaat catcaggtaa
      541 ccatcccgat ttctttcgta aaaagtgaag aaacgggtcg tataaaaaat ttcaaggtag
      601 cagccaagct tgaacctgga ggattttgtt ctacatatcc ttacgacgat tccgacgtgt
      661 tcaaaatcat cgaaggagct agttattcct tgcaattatt tcccgatcca caactggaag
      721 caaaactcga tacattgatc aattacattg ctctagctca agaacccgac ggttatcttt
      781 ataccaaccg caccatcgat tctctacata cacatccttg ggtagggaaa aaaagatggg
      841 aaaacgatcc caagcatagc cacgaattat acaatgtcgg gcatctttac gaagctgccg
      901 tggctcatta tttagctacc ggaaaaacta cacttctgaa tgtggctata aaaaacgccg
      961 atttggttgc gaaagatttt ctcgataaac gattgcccta ttatcctggg catcaagaaa
     1021 tagaactggc tctggtaaag ctctataaac tgaccggaaa ttctcgctat ctcgatctgg
     1081 ccaaatattt tctcgatatc cgtaaaggag gtaatgaata taaccaagcg catttacccg
     1141 taaaccagca aaccgagatt gttggccacg ccgttagagc cacctatatg tattctgcca
     1201 tggccgatgt agcaacttta aagaatgtcc ctgaatatca ggctgctatc catagaatgt
     1261 ggaatgactt gatcaatggg aaatattata taacgggtgg tataggttca cgagcaagca
     1321 acgagggctt tggaaacgct tatgaactac ccaacgaaga agcttattgt gagacctgtg
     1381 cgtcgattgg caacgtattg tggaattatc gtatgttttt aatggatgcg gatgccaaat
     1441 attacgatgt attggaacgt actctttata atgctttact ttcgggcgtt tcattgagtg
     1501 gcgacagatt tttttattca aatcctctcg aatctcatgg ccaacatgaa cgccaagcct
     1561 ggttcgattg tgcttgctgc ccaagtaatg tttgcagatt tttaccttca ttgccgggtt
     1621 atatttatgc tctggattct tcgactctct atgtcaatct cttcattcaa aaccaggcca
     1681 caatgcctgt cttcaatcat caaacaggta tcgaaatttt gaccaattat ccatgggatg
     1741 gaaaagtaca gattagaata ttagactcga atcctgttcc ctatgcaatt gccgttcgta
     1801 tcccaggatg ggcaagagga gaagccgttc cttccaattt atataagttt gctgatgata
     1861 atatttccaa atggaaactt cgggtcaata ataaggaata tcccttcgaa ttatcgaaag
     1921 gttatgtgtt tatcaataag gaatggaata gaggggatga aatcgaactt gaattaccca
     1981 tggaggttcg catggtaaaa gcccattcta ccgtaaaagc cgactctgga aagatagctc
     2041 tgcagagagg ccccctggtt tattgcctcg aatgggctga ccagcccggg aaagaccttt
     2101 ggaatatcgc cttgaaccta cagaaaggtt ttgagataat tcgggatgac tcattgcttc
     2161 caaattgtat aaatattaaa ggtgaggctt gtcagatcga gaaatttgcc agcgacaaat
     2221 gtttcgaatt caatgctatc ccctattttc tttgggcaca tcgcgggaaa ggccctatga
     2281 gggtttggat tccctacttc ccttcccgtc attagcttac ttggacttga tctgcaaacc
     2341 aaattcttga ttgtggctat caacgtggtt tatatcctga ctgattgaag agcaccgcat
     2401 aatcttcctc ctggatgatt tccttgagat tcttgtcggg atgtttcctg gcataagatt
     2461 caatgattct caaacccata tactgaccaa tacgaccggg acttcctttt ggaaatcctt
     2521 gaattgtagg actttcttga ataaatttca tgattatgct tttatcttta ctaaaaagta
     2581 gatcctggtt caatagaaac gaccagattt ccttttcgtt atcctgacac catttatatt
     2641 tttcggcagt gtaccccatg agaaaatatg gtggagtttt gggaagaata ttttgagcaa
     2701 aaaacaataa ttgtccatag gctatcatct gatcaaccag agctccatcg acaggaaaag
     2761 gaggtaaaaa ttgctgtgct atcgttttaa taatgtcggg aatcaaataa tctggcgtca
     2821 ttctctcagc aatgtattta ggtatgccaa tggttctata tcccggaaaa ttacttccta
     2881 gataattgtc gagcggaata atcaaaaccg agtcatcgat aaatattcct tcggctaaat
     2941 cgtagccact gatgtaagaa tataccgatt tgggtggatg ccatgaaggg aaataataat
     3001 ggatataccg aaaagcttca gttaaaccat cttcgatttg cgccaaggaa ggaaatgttt
     3061 tacaggcttg aacataagcc tctataattt gaggatcatt caaatattgc cgcatttgca
     3121 gcaaacttgc cggattatca tactcattcc ctataaataa gggatatttg tttgccaaat
     3181 gcttcagcaa ttcttgcaag ctatcgatag gaatacgaaa taaatcctgt tcgtatcgtt
     3241 gaatgctaac tttttgaatc tcaattttat taagttcggg tttatccatt ttatttctgc
     3301 aatataatcc cgaaaaagct aaaagtgaga ctactgcaaa tgaaatgatg atgtatttcc
     3361 gcataggtgt aatgtaaata atagaagacc atgatttgta gcatatagaa aagaagccca
     3421 gtaatgattc atgctgggct tcatggggta taaaccttca tgtgagcttt taaagtattt
     3481 tccaacctaa tgtaatatta aaaagattgt tcttgataga ttgaccggga gtattggaga
     3541 tattatttaa gcctaattcg tacctcatgt cgagtgtaaa catcaatata tccacccc
//
LOCUS       DAOORU010000195         3593 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_30916_length_3593_cov_5.614754, whole genome shotgun sequence.
ACCESSION   DAOORU010000195 DAOORU010000000
VERSION     DAOORU010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30916_length_3593_cov_5.614754"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1380
                     /locus_tag="PLF20_09590"
     CDS             208..1380
                     /locus_tag="PLF20_09590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOX81289.1"
                     /translation="MKYLTRTIWILSLVSLFTDIASEMLYPVMPIYLKSIGFTVVLIG
                     VLEGLAEATAGLSKGYFGKLSDEKGKRVPFIRIGYGLSAISKPLMAVFIYPLWVFFAR
                     TLDRLGKGIRTGARDALLSDESTPETKGRIFGFHRSLDTVGAAIGPLFALLFLYFYPN
                     QYKTLFFIAFIPGLLAIVLTFLIKEKPSIQKSSHNKVSFFSFINYVKTSNNTYKKIIA
                     GLLFFTLFNSSDVFLLLKIKEAGLNDTWVIGTYIFYNMVYALASYPLGIIADRLGLKK
                     IFVFGLLMFSIVYFGFAFNTKFTIFLGLFVVYGIYAAATEGISKAWITNVSPKGETAT
                     AIGAYTAFQSVFTMLASSLAGLLWYNFGATVALLTTAITSLITVIYFIFVVKPKTV"
     gene            1446..2231
                     /locus_tag="PLF20_09595"
     CDS             1446..2231
                     /locus_tag="PLF20_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81290.1"
                     /translation="MISSSNSIDAHTDEKFAPDSLQTLLVRILSGSDTIDDSILYFPR
                     SIELRLITNNEKAEIFYSLKNTINEIGFLQYHQPLLFNSSTTLLAYAILPSHIKSTMK
                     ELRLVKIENLFDFQIFCDERRCCQSSRRYKLFDGVTDNSETAENGNDYLCLKGSKIEF
                     WVDFGIKRQVNEVKFHLYVAETKNLILPSILVEGSENGYEFYPLGQKRFSVTFNKSFW
                     VKVELQPAKVRYIRIGFYNSSQSIENDCILIDEIHFDSKEEEF"
     gene            complement(2326..2976)
                     /locus_tag="PLF20_09600"
     CDS             complement(2326..2976)
                     /locus_tag="PLF20_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013263964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOX81291.1"
                     /translation="MMKVENIHKRYGTVEVLKGIHLEVYSKEILAIVGSSGAGKSTLL
                     HIMGTIEKPDQGKVFFKQMDLFKMKEKQLSAFRNQQLGFVFQFHHLLFEFTAIENVCI
                     PALIAGKDKNAIKKKAEEWLAFFGLAHRKDHKPTELSGGEQQRVAVARALINDPPLIL
                     ADEPSGNLDSKNAEELHRLFFELRNQYEKTFVIVTHNPSLSDLCDRRLLIKDGILQ"
     gene            complement(3145..>3593)
                     /locus_tag="PLF20_09605"
     CDS             complement(3145..>3593)
                     /locus_tag="PLF20_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81292.1"
                     /translation="IVAQKRVELGGNIGLQFGTVTIVNISPSAGYWFTNYLMGGIDLT
                     YLYSHDSRKGGYKGSTYGIDIIGKLYPYHNFYLEANYSNLNTGLSSIGVVKDRIWTKA
                     LLIGGGYSQQLSEKVFLNFSILWDVIGEEWYPFDNPLIRAGIVTRL"
BASE COUNT         1060 a          648 c          644 g         1241 t
ORIGIN      
        1 taattagtta tcaaaaagtt attaacaatt tgagtttata gacagactct aactaactga
       61 ataagtggta tataaaaatg tttaggaact ttttagaatt ttgtcggtta atagtgatta
      121 tttatatatg gcataaatta ccgcttcttt gaaagttgct atcaagattt ttctatattt
      181 gcaggtttga ggaatgaaaa tttctttatg aaatatctaa ctcgtaccat ctggatatta
      241 tcgcttgtaa gtttatttac tgacattgca agtgaaatgc tttatccagt tatgccgatt
      301 tatttaaaaa gcataggatt tacagtagtt ttaattggcg tattggaagg tttggccgaa
      361 gcaaccgccg gtttgagcaa gggttatttc ggtaaacttt ccgatgaaaa agggaaaaga
      421 gttccattta tcaggattgg atacggcctg agtgcgatat ctaagccttt gatggcggtt
      481 tttatatatc ccttatgggt gttctttgcc cggacactcg accgcttagg aaagggcata
      541 cgtaccggag cacgcgatgc attgctttcc gacgaatcca cacccgaaac caaaggtagg
      601 atttttgggt ttcatcgttc tttagatacc gtgggagccg ctattggtcc tttgtttgct
      661 ctcctttttc tttattttta tcccaatcaa tataaaaccc tcttttttat tgcttttatt
      721 ccaggtttac tggctattgt tcttactttc ctcatcaaag aaaaaccttc tattcaaaaa
      781 tcctcccata ataaagtttc ttttttctca ttcatcaact atgtgaaaac gagcaataat
      841 acttataaaa aaattatagc tggtttattg ttctttacgc ttttcaacag ttccgacgtt
      901 tttttattac tcaaaataaa ggaagcagga cttaacgata cctgggtgat tggaacctat
      961 attttctaca acatggttta tgcattggct tcatatccct tgggaataat tgccgacaga
     1021 ttggggctga agaaaatatt cgtttttggt ctcctaatgt tctccatcgt ttattttggc
     1081 tttgccttca atacaaaatt tactattttt ttgggattat ttgtggttta tggtatttat
     1141 gctgctgcta ccgagggaat tagcaaagca tggattacca atgtttctcc aaaaggagaa
     1201 acagctacgg ccattggagc ttatacagct tttcagagcg tttttaccat gctggcgagt
     1261 tcattggctg gcttgctttg gtataacttt ggcgctaccg ttgctttgct tactaccgca
     1321 ataacttccc tgattacagt aatatatttt attttcgtgg taaaaccaaa aacagtttga
     1381 tgatcctaca caataaaact tcttgcttac ttacaattat tcaattttta acaatttacg
     1441 cattggtgat ttcgtcatcc aattctatcg atgcccatac tgacgaaaaa tttgcacctg
     1501 atagcctaca aactttatta gtccgaattt tatcgggaag cgataccatt gacgattcca
     1561 ttctgtattt tccccgatcg atagaattaa gactgataac caacaacgaa aaagcagaaa
     1621 ttttttattc gttgaagaat acgataaatg aaataggatt ccttcaatac catcaaccgt
     1681 tgttgttcaa ttcttctact acacttctgg cttatgctat attaccatcc catataaaaa
     1741 gtactatgaa ggaattgagg cttgtaaaaa ttgaaaactt atttgatttt cagatttttt
     1801 gcgacgagag gagatgttgc cagagttcac gacgttacaa attgttcgat ggcgtaaccg
     1861 acaattcaga aactgcagaa aatggaaatg attacctgtg tttaaaagga tcaaaaattg
     1921 agttttgggt ggattttggg ataaaacgac aagtaaatga agtaaaattt catctttatg
     1981 tggcagaaac gaagaacctt atcttacctt caatattggt agaaggttca gaaaatggtt
     2041 atgaatttta tcctctggga caaaagagat tttctgttac ttttaataaa tctttttggg
     2101 tgaaagtgga attacagcct gcaaaagttc gttatatcag aattggtttt tataattcat
     2161 cacaaagtat tgaaaatgat tgtattttga tagatgaaat tcatttcgat tcgaaagaag
     2221 aagaatttta aggtatcggt ataatgttca ttttcaccta gtacagtttg catcagataa
     2281 gagacaatgt tttcgagaag aagaaaatgg aaatttcacg aaaaatcatt gtaatattcc
     2341 atccttgatt aacagacgcc tgtcacatag atcggaaaga gaaggattgt gagttacaat
     2401 gacgaaggtt ttttcgtatt ggtttcttaa ttcgaaaaac agccgatgga gttcttcggc
     2461 atttttgctg tcgaggtttc ccgagggttc atcggccaga ataaggggag gatcattgat
     2521 taaagcgcgt gctacggcaa cacgttgttg ttctccaccc gaaagttcag tgggcttatg
     2581 gtctttgcgg tgggctaatc cgaaaaaagc caaccattct tcggcttttt tctttatggc
     2641 gtttttatct tttcctgcaa taagagctgg aatacataca ttttcgatag cggtgaactc
     2701 gaaaagcaaa tgatgaaatt gaaaaacgaa tcctaattgc tgattacgaa aggccgataa
     2761 ttgcttttcc ttcatcttga ataaatccat ttgtttgaag aaaacttttc cctggtctgg
     2821 tttttcgatt gttcccataa tatgcagtaa ggtagatttc ccagcacccg aggaccctac
     2881 gattgctaga atttctttgg agtatacctc cagatgaatt cctttcaaca cttccactgt
     2941 tccatatcgt ttgtggatat tttccacttt catcatcgtt tccattttat aaactttaat
     3001 gctttattgc cttcttgcca tgttttatat ttgtatttgc actttgagtc gtcaacttgc
     3061 ggatctgatc aacttttcta taaatctata aaatttacct atcgtaatac agatttcccc
     3121 tttattgttt atcattacaa tgacttataa tcgagttaca atacccgccc taatcaaagg
     3181 gttatcgaat ggataccatt cttctcctat aacgtcccac aagatactaa aattaagaaa
     3241 aactttttca ctcaattgct gactataacc tccaccgatc aataaagcct tggtccatat
     3301 cctgtccttc accactccta tcgaggataa acccgtgttt aaattggaat aatttgcttc
     3361 gagataaaaa ttgtgatagg gatacagttt cccaataata tctattccat aagtacttcc
     3421 tttatagccg ccttttcttg agtcatgaga ataaagatag gtgagatcta tgcctcccat
     3481 taaataattc gtaaaccaat atcccgctga aggggagata tttacaatgg ttactgtgcc
     3541 gaattgcaat ccaatatttc ctcccaattc tacgcgtttt tgggctacga tgg
//
LOCUS       DAOORU010000196         3587 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_30985_length_3587_cov_5.860985, whole genome shotgun sequence.
ACCESSION   DAOORU010000196 DAOORU010000000
VERSION     DAOORU010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30985_length_3587_cov_5.860985"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..1951
                     /locus_tag="PLF20_09610"
     CDS             86..1951
                     /locus_tag="PLF20_09610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOX81293.1"
                     /translation="MNLISIENLTKSVCDTLLFSNLSFGLEQGQKAAFIARNGTGKTT
                     LLNIIAGLDTADEGKVIFRQSSKLAYLQQEVRLEPQQTIIEWLLDFNLELAELINHYE
                     SLVASAEPDRAEIERIIGEMEEARAWDYESRLKEIAGKLGFQNLQRRTGELSGGETKK
                     LALVKVLLAEADLLLLDEPTNQLDIKMIEWLEDYLSRQKLSLLLVSHDRYFIDRVCNE
                     IIELDRCKLFHYKGNYAYYLEKKSEKDEAETIEIEKVRNRYKTELEWMKRMPKARGTK
                     QKARIQAFFDLEEKINRPASSPQANFMASPQRIGKKILELHHIHKSFDGKAIMTDFSY
                     TFKKSEKIGIIGPNGAGKTTLLNIIMGKIKPEKGHISKGETIKFGYFTQERLELDSDQ
                     RIIEIVKNQAAEIPTPMGNLSASQFLQYFGFSYSSQYTYYSSLSGGEKRKLQLLLTLI
                     KNPNFLILDEPTNDMDIFTLNQLEIFLENYTGCVLAVSHDRFFIDRIADHVFAFEGNG
                     RIIDFPGNYSQYIATRKTELTNKNQPRTSKQPEVQKIRKGLSWKEKNELQNLEIEIES
                     LEMQKDELLETMALHSQHHEKLFEISKKYKEIETLIEEKITRWIFLSEKNEDFNS"
     gene            complement(1948..2529)
                     /locus_tag="PLF20_09615"
     CDS             complement(1948..2529)
                     /locus_tag="PLF20_09615"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008619176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOX81294.1"
                     /translation="MNESLFFEDDVRQAVAVLQGGGVILYPTDTIWGIGCKANDTRAI
                     SRIYTIKQRYEKKNLIILVDSEEMLRRYVTEVPAVASELIHTYSKPLTIVYPKAQNLP
                     SILVANDQSIAIRVVKHPFCIEVIRRIDAPLVSSSANLSGEPAPASYSSITDQIKQAV
                     DYISTTDRDKLYKPAASTLIRILPDDNFETLRD"
     gene            complement(2522..3523)
                     /locus_tag="PLF20_09620"
     CDS             complement(2522..3523)
                     /locus_tag="PLF20_09620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOX81295.1"
                     /translation="MNKIILIQTASIGDVILTTPVLEKLHYYFPEAIIDVVVKEGMES
                     LFAKHPFVGKVWTWEKRKHKYRHLFSLIKQIRTVHYDLAINCQRFASTGFLTVFSGAV
                     ITTGFHKNPFSAFFTYRIRHQIGNVHEVERNLSLIDFIGPEKRFSVRLYPSEADFNEV
                     VHLKYRPYICIAPASLWFTKQFPESKWIEFVGALPEDLQVYLLGAADERALCERIVKA
                     CVPRGVISLAGELSLLQTAALMQDAILNYVNDSAPLHIASSVNAPVAAIFCSTILEFG
                     FGPRSDRSFMIQTSQTLTCRPCGLHGYKKCPEGHFDCAYSILNQQLIQCISYVKCYE"
BASE COUNT         1200 a          739 c          720 g          928 t
ORIGIN      
        1 gtacgttgat tacttataga acaatcatgg ccttgatttt tgttgtgcgg cgtataaaaa
       61 ttattttttt atataagttt gtttcatgaa tttgatttct attgaaaatc tcaccaaatc
      121 agtatgtgat actcttcttt tcagtaacct aagctttgga ctcgagcaag gacaaaaagc
      181 tgcgtttatt gctcgaaacg gaacaggaaa gacgactttg ctcaacatta tagccggact
      241 cgacactgcc gacgaaggca aggtaatttt ccgccagagc tcaaaattgg cttatcttca
      301 gcaagaagtt cggctcgagc cccaacaaac cattatcgaa tggctactcg attttaacct
      361 cgaactggcc gaattgatca accattatga atcgcttgtt gcttcggcag aacccgatcg
      421 tgctgaaatc gaaagaataa tcggagaaat ggaagaagcc agagcatggg attatgaaag
      481 ccgcttaaag gaaattgccg gaaaattagg ttttcagaac ttgcaaaggc gtactggtga
      541 actatcgggt ggtgaaacga aaaaattggc tttagtaaaa gtattacttg ccgaagctga
      601 tttactatta ttggatgaac ccaccaatca gctcgacatc aagatgatag aatggctcga
      661 agattatctc agccgacaga agcttagcct gctgttagtc agccacgacc ggtatttcat
      721 cgacagggtg tgcaacgaaa tcatcgaact cgacaggtgt aaacttttcc attataaagg
      781 caattacgcc tattatctgg agaaaaaatc ggaaaaagac gaagccgaaa ccattgaaat
      841 agagaaagtc cgaaatcgat acaaaaccga actcgaatgg atgaaacgta tgccaaaagc
      901 aagaggaaca aaacaaaagg ccagaattca agcattcttt gatttggaag aaaaaataaa
      961 tcgacccgcc tccagcccac aagcaaattt catggcttca ccccaaagaa ttgggaagaa
     1021 aattctcgaa ctccatcata ttcacaagtc attcgacgga aaagccatta tgaccgactt
     1081 ctcatatacc tttaaaaaaa gcgaaaaaat tggcattata ggtccaaatg gtgcaggaaa
     1141 aaccaccctg ctcaatatca tcatgggaaa aataaaaccc gaaaagggac atatttcgaa
     1201 aggcgaaacc ataaaatttg ggtactttac acaagaaagg ctcgagctcg attccgatca
     1261 aagaattata gagatagtga aaaatcaggc cgcagaaatt cctacaccca tgggcaatct
     1321 ctctgcttct caatttctgc agtatttcgg attttcgtat tcttctcagt acacttatta
     1381 ttcttctctt agcggagggg agaaacgtaa actacaattg ctgcttacac tgattaaaaa
     1441 tcccaatttt ctcattctgg acgagcctac taacgatatg gacattttta ccctcaacca
     1501 gctcgagatt tttttagaaa actatactgg atgcgtttta gcggtttcac acgacaggtt
     1561 tttcatcgat cgcattgccg accatgtttt tgcattcgaa gggaatggaa gaatcataga
     1621 ttttccaggt aattatagcc aatacatcgc tacacgtaaa accgaactca cgaataaaaa
     1681 ccagccaaga accagcaagc aacccgaagt tcaaaaaata cgcaagggcc ttagttggaa
     1741 agaaaagaac gaattacaga atctggaaat tgaaatcgaa tcattggaaa tgcaaaagga
     1801 tgaattactc gaaaccatgg ctctacatag ccaacaccat gaaaaattat tcgaaataag
     1861 taagaaatat aaagaaattg aaacccttat agaagaaaaa atcactcgat ggatattctt
     1921 atctgaaaaa aatgaggatt ttaattccta atcgcgcaag gtctcgaaat tgtcatcggg
     1981 caaaatcctg atgagcgttg atgctgcagg tttgtataat ttgtcgcgat cggtggtgga
     2041 aatataatct acggcttgct ttatttgatc ggtaatcgaa ctatacgagg ccggagcggg
     2101 ctcacccgaa agattagccg acgaactcac taaaggagca tcgattcgac gtatgacttc
     2161 gatgcaaaac ggatgtttta ccacgcgtat ggctatactt tgatcgttag caaccagaat
     2221 cgagggaaga ttttgcgctt tcggataaac aatagtcaaa ggtttcgaat aagtatggat
     2281 caattccgat gcaactgcag gaacctcggt tacatacctg cgcaacattt cttccgaatc
     2341 tacaagaatt attaagttct ttttctcgta acgttgcttt atggtataaa ttcggctgat
     2401 ggctctcgta tcgttagctt tgcagccaat tccccaaatc gtatcggtgg gatacaggat
     2461 tactccaccc ccttgaagca ctgcaacagc ttgacggaca tcatcttcaa aaaacagact
     2521 ctcattcata gcatttaaca taggatatac attggataag ttgttgattc aatatagaat
     2581 aagcacaatc gaaatggcct tcagggcatt ttttgtaacc atgcaacccg caaggtcgac
     2641 atgtaagagt ttgtgaagtt tgaatcataa aagatctatc ggaacggggg ccaaaaccaa
     2701 attcgagaat tgttgaacag aatatggcag ctacaggcgc attgaccgat gaagctatat
     2761 gcaaaggagc agaatcgttt acataattca atatagcatc ttgcatcaga gcagctgttt
     2821 gtaataggct caactcaccg gccagagata tcaccccacg aggaacacag gccttaacaa
     2881 ttcgctcgca taaagcccgc tcatcagcag ctccaaggag gtaaacctgc aaatcttcag
     2941 gtaaggcccc gacaaattct atccatttgc tttcgggaaa ttgcttggta aaccataatg
     3001 aagccggtgc aatacaaata tagggacgat atttcaaatg aacgacctcg ttaaaatcgg
     3061 cctccgaagg atacaagcga acagaaaatc gtttttctgg tccaatgaaa tcaatcaagc
     3121 ttaaatttcg ctcaacctca tgcacatttc caatttgatg tctaatacga tacgtaaaaa
     3181 atgccgagaa aggattttta tgaaatccgg ttgtaataac tgcacccgag aaaactgtca
     3241 gaaaacctgt gctggcaaat cgctggcagt tgattgcaag gtcataatgt acggttctaa
     3301 tttgctttat caggctgaat aaatgacggt atttatgctt gcgcttttcc caggtccaaa
     3361 ctttgccaac aaaaggatgc tttgcgaaaa gggactccat tccctctttc actacaacgt
     3421 ctataatagc ttcagggaaa tagtaatgaa gcttctcgag cacgggggtg gttaaaatca
     3481 catctcctat cgaagcagtt tgtatgagga taattttatt catcaacaaa aacaaaccgg
     3541 aacaccgcaa ggataaagtg ctaatgattt acgctgccaa aaagggc
//
LOCUS       DAOORU010000197         3583 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_31037_length_3583_cov_5.897959, whole genome shotgun sequence.
ACCESSION   DAOORU010000197 DAOORU010000000
VERSION     DAOORU010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31037_length_3583_cov_5.897959"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2163)
                     /locus_tag="PLF20_09625"
     CDS             complement(<1..2163)
                     /locus_tag="PLF20_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOX81296.1"
                     /translation="MKTKIYFLILVLASIALLSMAASVLHTGSVQIEERGYSDYWRQI
                     DSLKELGQPATAARLTEELLSVALKENNSPQILKAHLYRIGLWAQYEEDYELKAIEAS
                     KKLINVTTMPAREILKSITAELYYLWYNNHQWELRGRGIMGDKLESDFRLWDARTFAD
                     TIASYYLASMDDPEKLYQIKAREFIPILDTAQGSEIFMPTLLDLLSHRALEFFSNEPI
                     ALTHSLEEFQPDHPDFFSPANSFIKVNLDKIQGTGFKKLALQIYRQLAALHLTYQEFP
                     PLIYNDLERIEFVYQNTGLSDKDSLYLQSLINLKNVYSENPHSTLVDHRLALWYYDKD
                     ELLKAANICRNAIEKYPETLGAKNCASLLNELYKPSLQIYCQNTFPPHSPILTQFFYK
                     NTDSIFYRIYRYDERKAGSRGMRQEDLFKNLLSKKYLRNIGVKVIDPGDLKVHSTEII
                     IEGLENGSYFLLASTGKNFDTIRDILVVQSLRISGLNAATSTLPDRRSVVYVVDRIQG
                     FPVADAAIKLFEQYYKPSTRTWEKHLAAETKTNLQGMAFFPLIRHSELKQPSCLIIFQ
                     SDTLYLENFFPQNIYNPVKTPIKRVYLFTDRKIYRPGQTLYYKGIFTETTGKQYQLLT
                     NEQCNVQLFDANGKQIEQKAHKTNRFGSFSGRMTLPSAGLNGSYSLVTPYGSVVFLVE
                     EYKRPNFEVSFESPSEVYKLGEKVSLRGKAIDYSGFPIG"
BASE COUNT         1095 a          668 c          664 g         1156 t
ORIGIN      
        1 cccaattggg aagccactgt agtcaatggc tttacctctc aggctcactt tctctcccag
       61 cttgtaaact tcactggggg attcgaaact cacttcaaaa tttggccgtt tatattcttc
      121 caccaaaaaa acaacgcttc cataaggtgt tacaagagaa tatgaaccat tcaatccggc
      181 cgaaggtaag gtcattcttc cgctgaaaga accaaaacga ttggtcttgt gggctttttg
      241 ctcaatttgt ttcccattgg catcaaataa ttgcacattg cattgttcat tcgtcagaag
      301 ttggtattgt tttcccgtcg tttccgtaaa aattccttta tagtaaaggg tttgccctgg
      361 tctatatatt tttctgtcgg taaaaaggta aactcgcttg atgggtgttt tcaccggatt
      421 ataaatattt tgagggaaaa aattctcgag atacagcgta tcgctttgaa aaattatcag
      481 acagctgggc tgtttgagct cactatgccg tatgagcggg aaaaaagcca tgccctgcaa
      541 attagtcttt gtttcagccg ctaagtgttt ttcccatgtt cttgtagaag gcttataata
      601 ctgttcgaat aattttatcg cagcatcagc aaccggaaag ccctgtattc tatcgactac
      661 ataaaccact gaacgcctgt cgggcaaagt gcttgtggct gcatttaaac cacttatacg
      721 taatgactga acgacgagaa tatccctgat ggtatcgaaa ttttttcccg tactggccaa
      781 aagaaaatag cttccattct cgaggccttc tataataatt tcggtcgaat gtacctttaa
      841 atctcctgga tcgattacct taactccaat atttcgcaag tatttcttag agagtaaatt
      901 cttgaataaa tcttcttgtc tcatccctct cgaacctgcc tttctttcat catagcgata
      961 aattcgataa aaaatagaat ccgtgttttt gtaaaaaaat tgagtgagaa taggcgaatg
     1021 tggcggaaaa gtattttgac aataaatctg aagacttggc ttataaagct catttaacaa
     1081 agaagcacaa ttttttgctc ctaaggtttc agggtatttt tctatggcat tgcgacatat
     1141 attagcagct ttcaacaatt catccttgtc gtaataccat agagccaaac gatgatcgac
     1201 caaggtagaa tgaggatttt cggaatagac gtttttgaga tttatcaagg actgtagata
     1261 aagactgtct ttatcgctca atcccgtatt ttgatacaca aattctattc tttccagatc
     1321 gttgtatatc aatggaggaa actcttgata agtcaggtgc aatgcagcaa gttgccgata
     1381 aatctgcaag gcgagctttt tgaatcctgt tccttgaatt ttgtctaagt ttaccttaat
     1441 gaaagaattg gctggagaaa aaaaatcagg atgatcgggt tgaaattcct caagggagtg
     1501 agtgagagca atgggttcat tgctaaaaaa ttccaaggca cgatgcgata ataaatcgag
     1561 aagggtaggc atgaaaattt cgctgccttg cgctgtatcc aaaataggaa taaattctct
     1621 ggctttgatc tgataaagtt tttcagggtc atccatcgaa gcaaggtaat aactggcaat
     1681 agtatcagca aaagttctgg catcccacaa tcggaaatcc gattccaatt tatcacccat
     1741 tattcctctc cctcttaatt cccattgatg gttattgtac cataaataat aaagttcagc
     1801 cgtaatactt tttaatattt cccgagcagg catagtggtt acattgatga gttttttact
     1861 ggcctctatg gctttcaact cataatcttc ttcatattgc gcccataagc caatacgata
     1921 aagatgtgct ttcagaattt gaggtgaatt gttttccttc aatgccacag atagcagttc
     1981 ttcggttaat ctggcggccg ttgcaggctg tcccagctct tttaaactgt cgatttgcct
     2041 ccagtaatca gaataacccc tttcttcgat ttgaacagag cctgtatgaa gtacggatgc
     2101 agccatactc aataatgcta ttgaagccaa gacgagtatt aaaaaatata ttttagtttt
     2161 cattccataa atttcaataa aaattaaggg gactactaaa aattcatttt ttattttttg
     2221 gttgagttga aaagttaagg caaatttata atcaattttt atcagttttg acaaattatg
     2281 acaatctttt gaaaaatttt agtgggaaac tctctatttc aattctatat ctatctgttt
     2341 ttcaataatt tatttcttta agatattcag tcgcacaatc tgctcatatc gaaacagatt
     2401 atatatcaaa ttggtcaaac ctataatcgc cttagctcaa aattttccta tcgttcccat
     2461 tacaatccat gcatgctttg ctccattatc gcaaatacat gcccaactct cgcccgtatt
     2521 cttaattttt cgtgattatt cgccttctgt tcttcggtta atggcttatt cctttatccg
     2581 ttctcgtgaa tgtgattgat catttgatat ttgactatta tttgctcttg tgtttttcca
     2641 acataaggtt tatctcccca tagtctatgc cctatatcct tgtaatttaa gatatctcca
     2701 agtacttgcg aatcatttaa agttgcatat ttactacgaa aaaaagacgc cataacgatt
     2761 aacggcatct caaccactat ttgagtttgt tgttgagaat taaaaagtga tgaattttga
     2821 acggataaaa aatagttata aaacgggatt tgttctgatt tcgatagatg aatacatcag
     2881 gaagcattta gagagtcagc caactgaaac tgaaaaggat cttaggaaac gattaaatgc
     2941 ggcattgaac gattataaga agggcataaa atgtttttgt ggtaacgata tttgggtgat
     3001 agggtcggct attgttagaa atgcttgctt tacgtgtata acaggcgaaa gttgcccaga
     3061 ccatgattat gaaatagatg aggcgatcat caagagacca tctacaaaaa gacgaaggca
     3121 tattgacacc atggataaaa gttaaattca tggacttttt aataacgatg gatttgaaat
     3181 taaccctaaa cttattaaaa agccttcatt atgtttgatt tgtaccaata atgacaatcc
     3241 caatgaagaa atactatgta atctgacccg ctacgaccaa agggataggg atgatttcac
     3301 atgctttgta tatataaaga gtatgattga ttcaaaaaaa cgcacgacgg tacaacgcgc
     3361 agtcataagc tttagtgaca caccaatgtt atcaatttct gcaatgctcc ccgatgcgag
     3421 gatttgtaat ccaaggcgag atgagttaca gttcttgaag ccatgaatca cgaaacgcag
     3481 ggattgcaaa tctcaaagaa ccgaaattgg atggttggtt ataacatttc cagcgggctc
     3541 ttgatgctgg atttggcaat atcggtaata tgcgtgtaga ttt
//
LOCUS       DAOORU010000198         3577 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_31129_length_3577_cov_5.417093, whole genome shotgun sequence.
ACCESSION   DAOORU010000198 DAOORU010000000
VERSION     DAOORU010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31129_length_3577_cov_5.417093"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3577
                     /gene="sprA"
                     /locus_tag="PLF20_09630"
     CDS             <1..>3577
                     /gene="sprA"
                     /locus_tag="PLF20_09630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOX81297.1"
                     /translation="DIYEATNIPLENGERGSVKWYQFKIPINDPQLIIGNIQDFKSIR
                     FMRMFFKNWQKPITMRFATFELVRGDWRRYRYDLLSPGEYIPNDEQSMTKFEISTVNV
                     EENGSKYPVPYVIPPGIEREINYGTTNFTRLNEQAMVLRVKELQDGDARAAYKTTGFD
                     FRQYHRLKMFVHAEDLDALNPNNYGDLTVFLRLGSDFTQNYYEYEIPLTFTPWGTAKT
                     NPALIWPDENSFDIDLDKLVQMKQERNIQMRDGETPISLTVPYIAYDGKNKVTIVGTP
                     SISDVRAIMIGVRNPKKKSFTDADDGQPKSTEIWVNELRLTDFNDQSGWAATGRLTVN
                     LADLGNISVNLAHSTPGFGSIEKKVNERQKETITSYDFATNLQLGKFLPEKLGLRFPM
                     HVDYSTTVASPQYNPLDPDIKLKDELDSYDVSSQKDSIRKLTEDYTQRKNINFMNVRK
                     ERVGTKRQPMPWDVENFDFTYSYSELNRRNIDIEYDNKKIYRGGIGYTYMTRPKVYRP
                     FEKIGIVSRSKDLQLIKDFNFSLLPRMFSFRTDINREYTERKLRNKSQALILIEPTYV
                     KSYVWTRMYDYKHDLTQGLKLEYNANANALIDELPGSVDKDDYGYTVKEKKDRIHEEL
                     MGFGTMNQFNQVLNLNYTIPINKIRAFNWMTATARYQGTYNWTASPKSIQEVMGNKAE
                     NSNTIQLNSNLRFTSLYNKVPYFKKVLSTTQTQRGGPQPARPGQKPQPTLQKPSREDT
                     AKVAKPKKDYFKIISESMVKIVLGFKDGSLSYSETNGIFLPGFKPSPGIIGNDMNLNA
                     PGIPFVFGSQADIRQKAAMNGWLTEDSLFNMAYATKHTSTLNARLTYEPFKDFRVEFT
                     ANRTYALNHQEYFRVNNGMYNSYSPIESGSFSISWFSLNTSFKSPDKNNVSKPFENMK
                     SMRKEIAMRLAQQNPNWQNDPQIVDSTGFPVGYGPTSQEVLHYSFLAAYSRQNAMKIN
                     LNAFPKIPYPNWRITYKGLSSVSFIKKYFKEFTLSHAYRSTYNVGSYSTNVLYTENFG
                     APSTFDDINNFIPKYQISQIALSEQFVPLFGIDMTWINNVLSKFEFRKSRNLALSFVN
                     NQLTEINSNEFVVGIGYRFKNIEMTFKGGGGGPTQRYKSDLNLRADFSIKDNKTIIRR
                     IEEDITQVSAGQKLITVNTSADYQLNERVMIRVFYDSSITNPYVS"
BASE COUNT         1161 a          747 c          712 g          957 t
ORIGIN      
        1 ccgatatcta cgaagccacg aatattccac tcgaaaatgg agagcggggc tcggtaaaat
       61 ggtatcaatt taagatccct attaatgacc ctcaactcat cattggaaac attcaggatt
      121 tcaaatctat ccgcttcatg aggatgtttt tcaaaaactg gcaaaaaccc atcactatgc
      181 gttttgcgac ttttgaactc gtaaggggcg attggcgtcg ttatcgatat gatttgcttt
      241 cacctggcga atatatccct aacgatgaac aaagcatgac caaatttgaa atctctacgg
      301 taaatgtcga agaaaatgga agtaaatatc cggttcctta tgtaatccca ccaggcatcg
      361 aaagagaaat taattatggg actaccaatt tcacccgcct caacgaacaa gcaatggttc
      421 tgagagtaaa ggaacttcag gatggtgatg caagagctgc ttataaaaca accggcttcg
      481 atttcagaca atatcacagg ctgaaaatgt ttgtccatgc cgaagacctc gatgcattaa
      541 atcctaataa ctatggcgat ttgacagttt ttctgcgttt gggctccgat tttacccaaa
      601 actattacga atacgaaatc cctttgacct ttacgccatg gggaacggca aaaaccaatc
      661 ccgctttgat atggcccgac gaaaacagtt tcgacatcga tctcgataaa ctggtacaaa
      721 tgaagcaaga acggaatata caaatgaggg atggagaaac tcctatatct ttgaccgttc
      781 cttatattgc ttatgatggg aaaaataagg taaccatagt tggtacacca agtattagcg
      841 atgtgagagc catcatgatt ggagtgcgca atcccaagaa aaaatccttc actgatgctg
      901 atgatggcca gcccaagagt accgagatat gggtaaacga attaaggctc accgatttta
      961 atgatcaatc gggatgggca gctactggcc gcctaaccgt taatctggcc gacctcggta
     1021 atatttcggt aaatttagcc cacagcacac cgggttttgg aagtatcgaa aaaaaggtaa
     1081 atgaacgaca gaaagaaacc attacatcgt atgattttgc cactaatctc caattgggga
     1141 aattcttacc cgaaaaatta ggtttacgat ttcccatgca tgtcgattat tccaccactg
     1201 tggcaagtcc acaatataat cctctcgatc cggatatcaa gctaaaggat gaactcgaca
     1261 gttatgacgt ttcatcgcag aaagactcga taagaaagct aacggaagat tatacccagc
     1321 gaaagaatat caactttatg aacgttcgaa aggaacgtgt aggaacaaaa cgtcagccca
     1381 tgccttggga tgtggagaat ttcgatttta catattctta cagcgaacta aaccggcgca
     1441 acatcgatat agaatatgac aacaagaaaa tctacagagg aggaatagga tacacctata
     1501 tgaccagacc aaaggtttac cgacctttcg aaaaaattgg catcgttagc cgcagcaaag
     1561 accttcaatt gataaaagat tttaactttt cgcttttgcc acgaatgttc agtttccgaa
     1621 ccgatatcaa ccgtgaatac accgaacgca aacttcgcaa caagagtcag gctttgatat
     1681 tgatcgagcc tacctatgta aaatcgtacg tttggactcg tatgtacgat tataaacacg
     1741 accttacaca aggtttgaaa ctcgaatata atgccaatgc caatgctttg atcgatgaac
     1801 tgccaggaag tgtggacaag gacgactatg gctataccgt gaaggagaaa aaggaccgca
     1861 tacatgaaga attaatgggt tttggaacca tgaatcaatt taatcaggta ttaaatctta
     1921 attataccat tcccatcaat aagattcggg cttttaactg gatgactgct acggcacggt
     1981 atcagggaac atataactgg acagcttcgc caaaaagcat tcaggaagtc atgggaaata
     2041 aagccgagaa ttccaacact attcaattga acagcaacct gaggtttacg agcctttaca
     2101 acaaagtccc ttattttaag aaggttttga gtacgactca aacccaaaga ggtggtcctc
     2161 agccagcgcg accggggcag aaacctcagc ctacactaca aaaaccctcc cgtgaagata
     2221 ccgcaaaggt cgctaaacca aagaaagatt acttcaagat catttctgaa tcgatggtaa
     2281 agattgtgct cggtttcaag gatggatcac tttcgtattc cgaaactaat ggcatttttc
     2341 taccggggtt taaaccatct cctggaatta taggcaacga catgaatctg aatgcgccgg
     2401 gaattccttt tgtttttggt agtcaggccg atatccggca aaaggcggcc atgaatggat
     2461 ggcttaccga agatagcctg tttaacatgg cttatgccac caagcataca tccaccttaa
     2521 atgctcgatt gacttatgaa ccttttaagg attttagagt ggaattcact gcaaaccgca
     2581 cttatgcgct aaatcatcag gaatattttc gggtaaacaa tggcatgtat aattcttatt
     2641 cacccataga atcgggtagc ttttctatct catggtttag cttgaatact tcttttaaat
     2701 cgccggataa aaacaatgtt tccaagcctt tcgaaaatat gaagtccatg cgtaaagaga
     2761 tagccatgag gctggcacag caaaatccca actggcaaaa tgatcctcaa atcgttgatt
     2821 ctactgggtt ccctgtgggt tatgggccaa cttctcaaga agtcttgcat tattcgtttt
     2881 tagctgctta ttcaaggcaa aacgctatga aaatcaatct gaatgccttc ccaaaaattc
     2941 cctaccccaa ttggcgaatt acttataaag gactttcatc ggttagtttt atcaaaaaat
     3001 attttaagga atttacactt tcgcatgctt accgctcaac ctacaatgtt ggaagttact
     3061 ctaccaacgt tttgtacacc gaaaattttg gcgcaccttc tactttcgac gatatcaata
     3121 attttatccc aaaatatcaa atttctcaaa tcgctctaag cgaacagttt gtcccattat
     3181 ttggaatcga tatgacttgg ataaataatg tactgtcgaa atttgagttt aggaaatcgc
     3241 ggaacctggc attgagtttt gtaaataatc agctcaccga aatcaattcc aatgaatttg
     3301 tagttggcat aggttaccgg ttcaagaaca ttgagatgac cttcaagggt ggtggtggcg
     3361 gaccaacaca aagatataag agcgatttga acttaagggc tgatttttct ataaaggata
     3421 ataaaacaat tatacgacga atagaagaag atataactca ggtttcggcc ggccaaaaac
     3481 tgattacagt caatacctcg gccgattatc agcttaatga aagagtaatg atacgggtgt
     3541 tttacgatag ttccatcacc aatccctatg tctccaa
//
LOCUS       DAOORU010000199         3510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_31921_length_3510_cov_4.433575, whole genome shotgun sequence.
ACCESSION   DAOORU010000199 DAOORU010000000
VERSION     DAOORU010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31921_length_3510_cov_4.433575"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PLF20_09635"
     CDS             <1..982
                     /locus_tag="PLF20_09635"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOX81298.1"
                     /translation="RASRKIHLAEEYTLPEVSALPERILVPVANPQSLQSLLELAIVL
                     KHPGSDEPIYPISIVEDNDQVQQNILKSRKNLDNIIQHISLSEVKIQPLVRVDVNIVS
                     GIIRGIKETMASMIVMGWGGSLKTSERIFGSILQKIISETEQTTLVSRIKQPVFIFKN
                     INVLCPPNVEYEVGFIYWVSKILIMAYRIQATLEFKTSKDLLPVFSTIARRWKINPSL
                     KNTIYSDWETGFESSSHSKTDDLFVIVAARKDTISFLPVFDSLPQRLNHLPENLNFIL
                     LFPEQVEKMKGTGVIPVSEIHISPIMSQMENLKKISKKVMKSVQKKSSHE"
     gene            975..1484
                     /locus_tag="PLF20_09640"
     CDS             975..1484
                     /locus_tag="PLF20_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81299.1"
                     /translation="MNKTFPLYLLSFLILLAACKTGRQTTQQPQQTAYSQTPAPLMVY
                     KTRYNYVNLVPIVLSQNKDSILHYPHPQDLKYGNSFRYPVLLEQGYLLDKKGIQPGTA
                     FLRLTYEEYAAFKDLPSQHQLLQMIADTNPFIELYRCKISLDESQRIDTLNAWIRNGQ
                     LGQKCSKLK"
     gene            1733..>3510
                     /locus_tag="PLF20_09645"
     CDS             1733..>3510
                     /locus_tag="PLF20_09645"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HOX81300.1"
                     /translation="MENRKGDSIEKQLNYIDGRALYYGFLAGANKIFENQHQLNKINV
                     FPVADADTGTNFASTMRSIIDMVEPNRDVKTTVDSLANAALIGARGNSGIIFAQFLYG
                     FSTGIGPTESLSVANFAMALKESVKYAYQAVTNPVEGTILTVIREWADFIYQIKDSIN
                     DFSILLENSIDKARISLEQTKIKLQVLARANVVDAGAKAFVLFLEGIIDLLKKGIKIL
                     LTSHQASKVEELSPIFHDEITFRYCTEALISGNGIDQQKIKKEMSFMGDSLVVAGSSS
                     KTRIHIHTDNPSGVFKRLMNHGQIIYQKVDDMAFQNTVASSQRSKVAILTDSTCDIPR
                     DLLDRYQIHVIPLNVHFGENYFLDGLSLSGKHFRKMFFSSKVYPSTSQPSIKDLMNRY
                     SYLFTQYDSVISLHISSAMSGTWNNSHQAATRIAVENRKMTTVIDSAKLSGSLGLLVL
                     RAAQMASEGALHNEIVEAIKQWKEKIYMLVSCTTTKYMVKSGRLSPMKGFIANVLNIK
                     PVIIVGSDGQTHENGKSVTESGAAKMVMAKIRKLLKTGEVWNYAITHFNNIQTANWYT
                     RELEKLIGKPPLFVSEGSPVLCINAGP"
BASE COUNT         1129 a          750 c          660 g          971 t
ORIGIN      
        1 tcgggctagc cgtaaaattc atttggcaga agaatatacc ctgcctgaag tctctgcctt
       61 gcccgagagg attcttgttc ccgtagccaa cccccaatcg cttcaatctt tgttagaatt
      121 ggcaatcgtt ttgaaacatc ccggatcgga tgaacccatc tatcctatat ccattgttga
      181 agataatgac caagtacagc aaaacatcct taaaagccga aaaaacttgg ataatatcat
      241 ccagcatatt tcactgagtg aagtcaagat tcaaccactg gtcagggtag atgtaaatat
      301 tgttagtggg attattagag gaatcaagga aaccatggcc agtatgatcg ttatgggctg
      361 gggaggaagc ttgaaaacaa gtgaacgtat ctttggaagt attcttcaga aaatcatttc
      421 cgaaactgag caaactactt tagtaagcag aatcaagcag cctgttttta ttttcaagaa
      481 tatcaacgta ttatgcccac ctaatgtaga atatgaagtg ggattcattt actgggtctc
      541 gaaaattttg ataatggctt acagaattca agcaaccctt gagttcaaga cttcgaaaga
      601 cctgttgccg gttttttcaa ccatagccag aagatggaaa atcaacccat ctcttaaaaa
      661 taccatctat tccgactggg aaacaggttt tgagtcgagt agccattcaa aaaccgacga
      721 tctctttgtt atagtcgcag cccggaaaga taccatatcg tttttacccg ttttcgatag
      781 tttgcctcaa cgattgaatc acttacccga aaacctcaac ttcattcttc tgttccccga
      841 acaagtcgaa aaaatgaaag gcactggagt aatcccagta agcgaaattc atatttctcc
      901 catcatgtcg caaatggaaa acctcaaaaa aatcagtaaa aaagtaatga aatctgtcca
      961 aaaaaaatca agtcatgaat aaaacctttc ctctttatct tctttcgttt ctcattctgt
     1021 tggcagcttg taaaacaggc cggcaaacca ctcagcaacc tcaacaaaca gcttattcgc
     1081 aaacgcctgc tcctctcatg gtatataaaa cacgttataa ctacgtgaat ttagttccca
     1141 ttgtgctaag ccaaaataaa gattccattc tacattaccc tcacccccaa gacctaaaat
     1201 atggaaattc tttccgctat cctgttttac tggaacaagg ctacctgctc gacaaaaagg
     1261 gaattcaacc cggcacggca ttcttaaggc ttacttatga agaatatgcc gcttttaaag
     1321 atttgccctc gcaacatcag ttgcttcaaa tgattgcaga caccaatcca tttattgaat
     1381 tataccgctg taaaatttct cttgatgaat ctcaaagaat agataccctg aatgcctgga
     1441 tacgaaatgg ccaattaggt cagaaatgct cgaaactaaa ataaacgacc tctgctattt
     1501 ttctgtatga ataatgacca tattatattt tgagtaattt tgcagccctt tcgaattgaa
     1561 aaatagacaa tcgttccata caaaatattg tatctcaagt ttggaattta ttttcttggg
     1621 aatgcacttt ctcttattac cgaagaacgc aaagatcctc agcctaaaga attttatcca
     1681 taatgaggtt ctatatagag tgaaggaata aaattttaat caagactaat ttatggaaaa
     1741 taggaagggc gactcgatcg aaaaacagtt gaattatatc gatgggaggg cattatatta
     1801 cggttttttg gcgggtgcca ataaaatatt tgaaaatcaa catcagctca ataaaatcaa
     1861 tgttttccct gtagccgatg ccgacacagg cacaaatttt gcctctacca tgcgctctat
     1921 catcgatatg gtagaaccca accgcgacgt aaaaaccact gtagattcgt tagccaatgc
     1981 cgcattaatc ggagcaagag ggaattcagg gatcatattc gcacagtttc tctatggctt
     2041 cagtacaggc atagggccca ccgagtccct gagcgttgca aactttgcca tggccttgaa
     2101 agaatcggta aagtatgcat atcaggccgt caccaatcct gttgaaggaa ctattttaac
     2161 cgtcatcaga gaatgggctg attttatcta tcaaattaaa gattccatca atgatttcag
     2221 catattgtta gaaaattcga tcgacaaggc cagaatatct ctggaacaaa caaaaataaa
     2281 attgcaggta ctggcaagag caaacgtcgt agatgccggt gccaaggcct ttgtattgtt
     2341 tttagaagga attatcgatc ttttaaaaaa aggaatcaaa attctactta cttctcatca
     2401 agccagtaag gttgaagaat tatcgcctat ctttcacgac gaaataacct tcagatactg
     2461 taccgaagct ctaattagtg gtaatggcat cgatcagcaa aaaatcaaga aggagatgtc
     2521 ttttatggga gattcgttag ttgttgccgg ctcttcgagt aaaacccgta tccacatcca
     2581 taccgacaac ccctccggag tctttaagcg cctgatgaat catggacaaa ttatttatca
     2641 aaaagtcgac gacatggcgt ttcaaaatac cgtcgcttcg agccaaaggt caaaagtagc
     2701 catacttacc gattctactt gtgacattcc acgtgattta ctcgatcgtt atcaaatcca
     2761 tgtcattcct ctcaatgttc attttggaga aaattatttt ctcgacggtt taagcttatc
     2821 gggtaaacac tttcgaaaaa tgttcttttc gtcgaaagtc tatccctcga cctctcaacc
     2881 ttccattaaa gacttgatga accgctattc gtacctgttt actcaatacg attcggtaat
     2941 cagcctgcat atcagttcgg ccatgagtgg cacatggaac aacagccatc aggctgccac
     3001 cagaatagct gtcgaaaata ggaaaatgac tacggtaatc gattcggcta aactttcggg
     3061 ttcattaggc ctactcgtcc tacgagccgc gcaaatggca tctgaaggcg ctttgcacaa
     3121 tgaaattgtc gaagctatca aacaatggaa agaaaaaatt tatatgctcg ttagctgcac
     3181 tactacaaaa tacatggtaa aaagtgggcg acttagccct atgaagggat tcattgccaa
     3241 tgtcttgaat attaaacctg taatcatagt cggaagtgat ggccagactc atgagaatgg
     3301 taaatcggta accgaaagcg gagctgcaaa aatggtcatg gcaaaaatca ggaaattgct
     3361 caagacagga gaggtttgga attatgccat cactcatttc aataatatac aaacggccaa
     3421 ttggtatacc agggaattgg aaaaactcat aggcaagcct cccctattcg tatcggaagg
     3481 ctcaccggtg ctttgcatca acgccgggcc
//
LOCUS       DAOORU010000200         3317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_34422_length_3317_cov_5.270386, whole genome shotgun sequence.
ACCESSION   DAOORU010000200 DAOORU010000000
VERSION     DAOORU010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34422_length_3317_cov_5.270386"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            269..1078
                     /gene="lgt"
                     /locus_tag="PLF20_09650"
     CDS             269..1078
                     /gene="lgt"
                     /locus_tag="PLF20_09650"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOX81301.1"
                     /translation="MLAYIYWNPSPVAFSLGPLQIRWYGLLFALPFVIGYFLMRDFYK
                     KEKQAFTYLDTLLTYVVLGAIIGARLGHCLFYEPEVYLRDPVRILKIWEGGLASHGGA
                     LGVILALWLYSCKTHQSFWWTTDRVAILVGMAGASIRLGNVMNSEIIGKPTDLPWAFI
                     FARVDKIPRHPTQLYESLAYLLIFFLMLYLYHRFLYKNRIIPPGYFVGLFLILVFTVR
                     FFIEFLKEPQVGFEQNLPLNMGQLLSIPFIIFGLWLVLRKHKTVANKIQSS"
     gene            1075..1707
                     /locus_tag="PLF20_09655"
     CDS             1075..1707
                     /locus_tag="PLF20_09655"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HOX81302.1"
                     /translation="MIFDLTHPIAEGMPVWPGTPSPKLEKECTIEKDGFLETHLGLSS
                     HTGTHIDAPAHILPGSYTLDKFRPEYFIGRAIKINLTHCHHTILEEEFLFLEDFPEAF
                     DFVLLQTGWSRYWGHPAYFRNFPVLSLQSARFLSSSQIKGIGLDAISFDLAESNTFPN
                     HHIFMGKGILLIENLTGLEPLPLGKSFELFALPLYFRSADGAPARVIART"
     gene            complement(1719..1940)
                     /locus_tag="PLF20_09660"
     CDS             complement(1719..1940)
                     /locus_tag="PLF20_09660"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOX81303.1"
                     /translation="MSDLVDHIDHIAKVAGIDHIGIGTDFDGGGGLTDCRDVSELPNI
                     TVELIRRGCSPEQIQKIWGGNLMRVMNAQ"
     gene            complement(1946..2353)
                     /locus_tag="PLF20_09665"
     CDS             complement(1946..2353)
                     /locus_tag="PLF20_09665"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOX81304.1"
                     /translation="MEQEEGLSDLAKEVVREMNRLGMMVDVSHISDKAFWNVISITTK
                     PVIASRSSARAICNHPRNLSDDMLKAIAQNGCVVQVCILSDYVKNIPPDSRYDSAYNI
                     LRERYHHFENLTPDEKNRFVEILIVFRSFIHVG"
     gene            complement(2514..2903)
                     /locus_tag="PLF20_09670"
     CDS             complement(2514..2903)
                     /locus_tag="PLF20_09670"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HOX81305.1"
                     /translation="MRRLTVWLIVCGGLFHLNCPAQNLPSDKHFHHNLLTIDSHPDTP
                     LMLLRPGTDFMQDNSANPYSRVDYPKMQKGGLDAVFFAVFIPQGALESASFDKAYSMA
                     RKMIFHIDSLVSHNSEKLGLAVSAEIS"
     gene            complement(2966..>3317)
                     /locus_tag="PLF20_09675"
     CDS             complement(2966..>3317)
                     /locus_tag="PLF20_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOX81306.1"
                     /translation="EAAAKGHERAIIALNMFRYRVKKYVGAYTAAMGGVDAIVFTGGI
                     GENDAASREEIMKDMEWLGIDFDFEKNRSVKSQENIITREDSKVKVMVIPTNEELVIA
                     SDTYEIVTRNLEMI"
BASE COUNT          906 a          732 c          679 g         1000 t
ORIGIN      
        1 caacttgctg tatacaagga agttacaacc ctaaggcgct caagttggga taagtgcatt
       61 atgtagcaag agcagatttc gagacttttg ctcgttatat ttccaatcca taatgtcttt
      121 gataagagga tgtgatcaga gaataatcca tcgaacatca cattcaaagc gggtttggag
      181 cataagaaat taatgctgta cgcccaaaat aaaacgccta aaatcgacga aacaaaaaat
      241 acagtaggtt tgcacaaaaa tcggtaatat gctggcttac atttactgga atccgagtcc
      301 tgtagctttc tcgttaggtc ctcttcaaat tcggtggtat ggtttattat ttgccctacc
      361 cttcgtaatc gggtattttt taatgcgtga tttctacaag aaggaaaaac aggcctttac
      421 gtatctggat actctgttga cttatgtagt cttgggtgca attatcgggg cgcgtttggg
      481 gcattgtttg ttctatgagc ccgaggttta tcttcgcgat cctgttagaa tccttaaaat
      541 atgggaagga ggcttagcca gtcatggcgg tgcacttgga gtcattctcg ccttatggct
      601 ttattcctgt aaaacacatc agagtttctg gtggactacc gatcgtgtgg ctattttagt
      661 cggcatggca ggagcgagca tacgtttggg taatgtcatg aattccgaga taataggcaa
      721 acccaccgac ttaccttggg ctttcatttt tgcgagagtg gataaaatac cccgacaccc
      781 cacccagtta tacgaatctt tggcttatct gctgatcttc ttcttgatgt tgtatctcta
      841 tcatcgtttc ctgtataaaa atagaatcat tccaccggga tatttcgtcg gactgtttct
      901 aattttggta tttacagttc gtttcttcat agagtttttg aaggaacctc aggtaggctt
      961 cgagcagaat ctgcccttga atatgggaca attgctcagt atcccgttta ttatctttgg
     1021 attatggctt gtgcttcgaa aacacaaaac ggtggcaaat aaaattcaat cctcatgata
     1081 ttcgatctta ctcatccaat tgcagaaggt atgccggtat ggccaggaac gccatcacca
     1141 aagctcgaaa aagaatgtac aatagagaaa gacggttttc tggaaaccca tctcggtctg
     1201 agttcgcata caggaacgca catagatgca cctgctcata ttttgcccgg gagctacact
     1261 ttagataagt ttcggcctga atatttcatc ggcagagcaa tcaaaatcaa tcttacacat
     1321 tgccatcata ccattttgga agaagaattt ttatttctcg aagactttcc cgaagctttc
     1381 gattttgtat tattacaaac ggggtggagc cggtattggg gtcatccggc ctatttcagg
     1441 aattttccgg tactttcgct ccagtcagcc aggtttctca gttcttcaca aataaaaggg
     1501 ataggtttgg atgccatttc tttcgatctg gctgaaagta acaccttccc taatcatcat
     1561 attttcatgg gaaaaggtat acttttgata gaaaatctca cagggctgga gcctttacct
     1621 ttgggaaaga gtttcgaatt gtttgctctt cctctgtatt ttcgctctgc cgatggtgct
     1681 ccggcccgcg tgattgcacg cacctaaccc cgaaagaatc attgcgcatt catcactcgc
     1741 atcaggttac caccccatat tttctggatt tgttcaggag aacatcctct acggataagc
     1801 tcgacggtaa tattgggtaa ttcgctcaca tccctgcaat cggtcaggcc gcctccccca
     1861 tcgaaatcag tccctattcc aatatggtca atacccgcca ctttagctat atgatcgata
     1921 tgatctacta aatcgctcac agtggctaac cgacgtggat aaagcttctg aagactatca
     1981 atatctctac aaaacgattt ttttcatcgg gagtgagatt ctcaaaatga tgataccgtt
     2041 ctctgagaat attatatgca gaatcgtaac gagaatcggg aggtatgttt tttacataat
     2101 cacttaaaat acatacttgt acaacgcaac cattttgtgc aattgccttc agcatatcgt
     2161 cgctcaagtt tcgcggatga ttacaaatag ctctggcaga gctacgagaa gcaataacgg
     2221 gtttggttgt aatggaaatg acattccaaa aggccttgtc cgaaatatgg gaaacatcca
     2281 ccatcattcc caaacggttc atttcacgca ctacttcctt ggctaaatca ctcaagccct
     2341 cttcctgttc catatcggta aagaagtaca aatctggttg ttcttcgaat ggcacaaagt
     2401 aatgtacctc actcccaaat cgtaaaattc ttttactctg tttattttat cagctatggg
     2461 gtatccattt tccatcccca tcaaaatact cgatttgccc tctgaatgat ttcttaggat
     2521 atctccgcac taaccgctag accgagcttt tccgaattat gagaaaccag ggaatcgata
     2581 tgaaaaatca tcttgcgagc catggaatat gccttatcga atgaggcgct ttcgagtgcg
     2641 ccctggggta taaaaaccgc aaaaaacacg gcatcgagtc cgcctttctg catcttagga
     2701 taatccactc tgctataagg attagcgcta ttatcctgca tgaaatcagt tcccggcctt
     2761 aataacatta agggagtatc tgggtgggaa tcaatggtta ataagttatg atggaaatgt
     2821 ttgtcagaag gtaagttctg agccggacaa ttcaaatgga aaagtccccc gcaaactatc
     2881 agccatacag taagtcgtcg catgtgaata tatttccgag ttagatgatc atcgaaggaa
     2941 atgatgcctt aatcctcgaa gaaggtcaga tcatttcgag atttcgggta acaatttcat
     3001 aagtatcgga ggcaatgacg agttcctcgt tggttggaat taccatgact tttaccttcg
     3061 aatcctcacg ggtaattatg ttttcttggc tcttcacgct acgatttttc tcgaagtcga
     3121 aatctattcc cagccactcc atgtctttca taatttcttc gcgcgaagca gcatcatttt
     3181 ctccgattcc acccgtaaag acgatagcgt cgacgccacc catagcagcg gtatatgctc
     3241 ctacatattt tttcacgcga tagcggaaca tattcagggc gattatggct ctttcatggc
     3301 ctttggcggc agcttct
//
LOCUS       DAOORU010000201         3296 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_34729_length_3296_cov_5.195310, whole genome shotgun sequence.
ACCESSION   DAOORU010000201 DAOORU010000000
VERSION     DAOORU010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34729_length_3296_cov_5.195310"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /locus_tag="PLF20_09680"
     CDS             <1..850
                     /locus_tag="PLF20_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOX81307.1"
                     /translation="LMKDDWPIFFEGLHTTYYLSHPFLKNRLKIYRESNIEHHYYWKL
                     SLASRKIIDKVFFAVEAARLFLWQKQLSYADLILTVSEDDSLYLRKKFPHKPVECIPS
                     FHGNDEVTSKAGFGNFALYHGKLSVPENDQSARFLIRKVFAGLPYRLIVAGMNPEKNL
                     IRLAKNYPNVVVMANPSLEEMDKLIEEAHINVLITQQNTGLKLKLLNTLFKGRHCLVN
                     DKMLVGTNLAQLCHVANDKNSLKEKITELFQQPFSPDDIGKRIHHLNHWYSDDENTRK
                     ILDLVF"
     gene            1265..3265
                     /locus_tag="PLF20_09685"
     CDS             1265..3265
                     /locus_tag="PLF20_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HOX81308.1"
                     /translation="MKQKLCALFFLIGITMEATAQQGGYSLKFDGSNNYVSVANASSL
                     NITDSITIEAWVKPSADGWIYKKPITLSPSTPIADYQVKVELTTSNFAYAHTNSDGSD
                     IRFYDGNGTELNYWIEKWDVSGTSIIWVKVPTSGTTEIFMYYGNSSAQSVSNATNTFV
                     RVINGNSPVKGSWHLDEGSGTMAYDMSGNGNNGTLYNSPTWVDGKFGKALSFSGNNWV
                     DCGTNSSLNTPPQFTQEAWIYPTITDNDYHGFLGYEDYNVPRSACLYVYQKYKIHAGF
                     GNNNGWYSFITDNIISENTWNHVVATFDGTYYKIYVNGVEKYSTSTLAGKTPSPTPIR
                     YIGRVNNYFIGRIDEVRIYNRALTSEEVSDLYQNYGYTTTNAPGQVFVRKYSNPEPIA
                     TVGNETAVGISKAGAYGIGANTTTAFASINNQTITGTISSGWNHIAQTYDGSTQKLYV
                     NGILVVFQDLSGAISPDANNLLIGDFFNGTIDEVRLWNVARTADEINENMYRELTGSE
                     TGLVAYYKMSNGSGSTVSDNKSGGTNTGIINGATWAASGALSGSKMALDFDGSNDQVQ
                     FQGNSPAYNNTALTIEAWMKSTNAGENKELVSWGDASGSNIVEFKMDKGKLKFGINVS
                     SAESVWGSQAINTGKWVHVAVVKDNSNITLYVNGVVVRHYKL"
BASE COUNT         1079 a          584 c          666 g          967 t
ORIGIN      
        1 cctgatgaag gacgattggc ctatcttttt cgaaggatta cataccactt attatctcag
       61 ccatcctttc ttgaaaaacc gtctgaaaat ttatcgtgaa agcaatatcg aacaccatta
      121 ttactggaaa ctgtctttag caagccgaaa gataatagac aaagtatttt ttgcagtgga
      181 agcggcaaga ttattcctgt ggcaaaagca actttcgtat gccgatctta tacttacagt
      241 ttcggaagat gattctcttt atctaagaaa aaaatttccc cataaaccag tcgaatgcat
      301 tcccagtttt catggcaacg atgaggtaac aagcaaagcc ggttttggta attttgcgct
      361 ttatcacgga aaactttcgg tgccagaaaa tgatcagtcg gctcggtttc ttattcgaaa
      421 ggtatttgcc ggacttcctt atcgcttgat tgttgccgga atgaatcccg aaaagaactt
      481 gattaggctc gccaaaaatt accctaatgt cgtggtgatg gccaatcctt ccttggagga
      541 aatggacaaa ctcatcgaag aagcccacat caatgtattg attacacaac aaaatacagg
      601 actgaaacta aagcttctga atacactctt caagggaagg cactgccttg taaacgacaa
      661 gatgctcgtc ggaaccaatt tggctcaact atgccatgta gccaatgata aaaactctct
      721 gaaagaaaaa atcaccgaat tatttcaaca acccttctca cccgacgaca ttggaaaaag
      781 gattcatcac ctgaatcact ggtattcgga tgatgaaaat acaagaaaaa ttctggattt
      841 ggttttttaa gttctaagac gaaacctcac aaaatttacg taacagccgg ttcacaccta
      901 accctgacgg attttataaa actgtggggt tttacatttt ttcacgccga ctgaaaacga
      961 acaacaccaa tcactgatat ccagtatttt acaaattgtt aataactttt ttataatttt
     1021 tttttcggaa acacttgata aataaaaatt tatgttttta atttgccatc aattactaat
     1081 tatggtagat atcttttcct ttacttaaat taaaaccttg gttttttcat tgaaagaaag
     1141 atagatatgt tgaataaaag gcaggaggaa aaacaagagt ttaattggtt acaataccat
     1201 atttagttaa ttatttaaaa gttaggtatt tatgagattt cttaaccaat aaaaccctaa
     1261 aattatgaaa cagaaattgt gtgccttatt tttcttgata ggtattacca tggaggcaac
     1321 ggcacaacaa ggtggttatt ccctgaagtt cgatggcagt aataattacg tttctgttgc
     1381 aaatgcatcc agtttgaaca tcaccgattc aattacgatt gaagcttggg taaaaccttc
     1441 ggccgatggc tggatataca aaaaaccaat cactctaagc ccctcaaccc ctatagccga
     1501 ttatcaggta aaagttgaat taacaacttc caatttcgct tatgctcaca ccaacagtga
     1561 tggcagcgat attcgttttt acgacgggaa tggaactgaa ttaaattact ggattgagaa
     1621 atgggatgtt tcaggtactt ccattatctg ggtaaaagtg cccacttcag gaacaactga
     1681 aatctttatg tattatggga attcttctgc ccaatctgtc agcaatgcaa ccaatacttt
     1741 tgtaagagtg ataaatggta atagtccggt aaaaggaagt tggcatttgg atgaaggcag
     1801 tggaacaatg gcttatgata tgtcgggtaa tgggaacaat ggtactttgt acaatagtcc
     1861 aacatgggta gatgggaaat ttggaaaggc gttgagtttt agtggtaata attgggtgga
     1921 ttgtggaacc aattcaagtt taaatacgcc gccgcaattt acacaggaag catggattta
     1981 tccaactatt actgataatg attatcatgg gtttttagga tatgaagact acaatgttcc
     2041 cagatcagcc tgtttatatg tttatcaaaa atacaaaata catgctggct ttggaaataa
     2101 caatggttgg tattcattta taacagataa tattatttct gaaaatacat ggaaccatgt
     2161 agtagctact tttgatggaa cctattataa aatttatgtg aatggcgttg aaaaatattc
     2221 tacttctaca ttagcaggga aaactccttc tccaactcca attagatata taggcagggt
     2281 aaataattat ttcataggta gaattgatga agtacgaatt tataatcgtg ccttgacttc
     2341 agaggaggtt tcagatttgt accaaaatta tggctacacc actacaaatg ctccgggaca
     2401 agtttttgtt cgcaagtatt caaatcctga gccaatagcc acggtaggca acgaaacggc
     2461 agtcggaatt tccaaagccg gtgcttatgg cattggtgca aacacgacta ctgcctttgc
     2521 ctcaataaac aatcagacga taacagggac gatttcttcg ggatggaatc atatagctca
     2581 aacgtatgat ggttcaaccc aaaaacttta tgtaaatggt attttagtag tttttcagga
     2641 tttgagtggt gctatttcac ccgatgcaaa taatctttta attggtgatt ttttcaacgg
     2701 cacaatcgat gaggttcggc tctggaatgt agcccgaact gccgatgaaa tcaatgagaa
     2761 tatgtatagg gagctcacag gaagcgaaac cggactggta gcttattaca aaatgagcaa
     2821 tggaagcggt agcactgtga gtgataacaa aagcggcgga acaaacactg gcataataaa
     2881 tggggcaaca tgggcagcct ctggagcttt atccggcagc aagatggcgc tcgattttga
     2941 tggaagcaac gatcaagttc aatttcaagg caattctcct gcatataaca ataccgccct
     3001 caccatcgaa gcttggatga aatctaccaa tgctggggaa aataaagaac tggtaagttg
     3061 gggagatgct tcgggcagta atattgttga gtttaaaatg gataagggca aacttaaatt
     3121 tgggataaat gtttcctcag cggaatctgt ctggggaagt caggccatta atacgggtaa
     3181 atgggtgcat gtggcagtag taaaagataa ttcaaatatc actttatacg taaatggcgt
     3241 agttgtgcgt cattataaat tgtgacagtt gcattgaaac aaaagtaata tcagtt
//
LOCUS       DAOORU010000202         3225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_35735_length_3225_cov_5.498107, whole genome shotgun sequence.
ACCESSION   DAOORU010000202 DAOORU010000000
VERSION     DAOORU010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35735_length_3225_cov_5.498107"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="PLF20_09690"
     CDS             complement(<1..477)
                     /locus_tag="PLF20_09690"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX81309.1"
                     /translation="MASNRKKSYIEKLIEKGENQHLDFKFAVNDSRKIARSMVAFANT
                     DGGRLLIGVKDNGVIAGVKTVEEFHMVETAAHIFSRPPIPFRSIEWNMGGKMILEIII
                     SKGRHRPYYAQDEHGQWTAWLRQSDRDIKAPKILTEYWTRKSSGEITTLSYGPFEKF"
     gene            539..>3225
                     /locus_tag="PLF20_09695"
     CDS             539..>3225
                     /locus_tag="PLF20_09695"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HOX81310.1"
                     /translation="MPGIVQIMLKNYPFSAVIFDLDGVITQTALVHSAAWKKMFDEFL
                     LNWSKKNETEFQEFEHNQDYLPYIDGKPRYQGVESFLKSRNIELPYGDPSDSPEMETI
                     CGLGNRKNLTFNEVLEKEGVKVYQSTIDLIYELRKNGIHIGVASSSKNCKPVLERAGI
                     LNLFETIVDGTVSAELGLKGKPFPDIFITACKNLNVSPNKAVVVEDAVSGVEAGKNGN
                     FGFVLGVARENNHRELLIHGADRVVSDLEELGGIKGMMDWFTNGLSTDNNSLIYYDLV
                     PEKEKQRETLLTVGNGYFATRGSMEETLASICHYPATYMAGVYNRLVSKVAGRDVENE
                     DFVNCINWLPVTFKISQGEWFDLDKAEIIEIQRELDFRSGIMKRKLTVKFEGKTTRIE
                     SERFVSMHQRNIAAQKYTIKPIDYDDEITIKAGLDIDLINDGVDRYRSLNQKHILPLE
                     EKVESNSGYLVGCTTQSNIRIACTYILDANKEGSWQPLVYKGKVFSLFTACIRSNEEV
                     TLTKRIVLNNSLSNDDPLTLVLVEGKNLKSYEDLRELSEVAWRNIWTRVDIELEGDRL
                     AQKIFRLHLFHLFVTASPHNASLDAGIPARGLHGEAYRGHIFWDELFILPLYNLSFPE
                     ISRSALMYRYRRLPAAREYARAHGYRGAMFPWQSGSSGREETQVVHLNPVSGEWGDDY
                     SSIQRHVSLAIAYNIWEYYHSTLDQQFLKDYGAEFFLDICMFWTGLCHKDAEDGRYHI
                     RGVMGPDEFHEKYPNAKRGGLDDNAYSNLMTVWALQKAYQMRNIIGEECFRNVARKIG
                     LNEQEWQRWKNIRQNLALCINEEGIIAQFDGYFELKELDWEYFKKKYGNVYRMDRLLK
                     AEGLSPDEFKVAKQADVLMIFYNLLPEEVNRLLQEMGYD"
BASE COUNT          940 a          584 c          732 g          969 t
ORIGIN      
        1 gaacttttca aatggaccat aagataaggt agtaatttct cccgaagatt tacgagtcca
       61 atattctgtc aatatttttg gtgccttaat gtcccggtca ctttgtcgaa gccatgcagt
      121 ccattgtcca tgttcatctt gagcataata cgggcgatgt cggcctttcg aaataataat
      181 ttccaaaatc atttttcctc ccatattcca ttcaatactt ctaaatggaa tcggaggccg
      241 tgaaaaaata tgtgcagccg tttctaccat gtgaaactct tcgactgttt tcactcctgc
      301 aataactcca ttgtctttca ctccaatcaa taaccggcct ccgtcggtat tagcaaaggc
      361 taccatagaa cgggcaattt tacgggaatc atttacggcg aatttaaaat cgaggtgttg
      421 attctcgccc ttctcgatta atttttcaat gtaagatttc tttctattgg aggccatggc
      481 ttatcaggct ttaagcgtcg ggtttagggc attaagttct tgcaaatatc ataattttgt
      541 gccgggaatc gtacaaatta tgcttaaaaa ctatcctttt tcggctgtta ttttcgattt
      601 ggatggggtt atcacacaaa cggccctggt tcatagcgcc gcatggaaga aaatgttcga
      661 tgaattcctt ttgaactgga gtaaaaaaaa tgagacggaa tttcaggagt tcgaacataa
      721 tcaggattac cttccttata ttgatgggaa accgcgttat cagggcgtgg agtcttttct
      781 caaatctcgg aatattgaac taccttacgg cgatccctcc gactctcccg aaatggaaac
      841 tatttgtgga ctgggaaacc gaaaaaacct tacattcaac gaggttctcg aaaaggaagg
      901 cgttaaagtg tatcaatcaa ccatagactt aatttatgaa cttcggaaaa atggaataca
      961 tataggtgta gcctcttcga gtaagaattg caagccagtg ttggagagag cgggcatttt
     1021 aaacctattc gaaaccattg tagatggaac tgtttcggct gaattaggat taaaaggtaa
     1081 accttttccc gatattttta ttacggcttg taagaatctg aatgtttctc caaataaggc
     1141 ggtcgttgtt gaagatgccg tgtcgggggt ggaagcgggc aaaaatggca acttcggatt
     1201 tgttttaggt gttgcccgcg aaaataatca tcgtgaatta ctgattcatg gtgccgatag
     1261 ggtagtaagc gacctcgaag aattgggtgg aataaaagga atgatggatt ggtttaccaa
     1321 tggtttgtca actgataaca atagcctcat ttattacgat ctggttcccg agaaggaaaa
     1381 acaaagggaa actcttttga cagttgggaa tggatatttt gcaacccgtg gctcgatgga
     1441 ggagacgtta gccagtatat gccattaccc tgctacttat atggctggtg tttataatcg
     1501 gcttgtttcg aaagtagcgg gaagagatgt cgaaaatgaa gattttgtaa actgtatcaa
     1561 ttggctgcct gtcactttca agattagcca aggcgagtgg tttgatctag ataaggccga
     1621 aatcatagaa attcaacgtg agttagattt tcgttcaggt attatgaaac gtaagttgac
     1681 agtcaagttt gaagggaaaa ccactcgaat cgaatctgaa cgctttgtct cgatgcacca
     1741 gagaaatatt gctgctcaga aatataccat taaaccaatc gattatgatg atgagattac
     1801 gataaaagca gggctcgata tcgatttgat caatgatggt gtggaccgct atcgttcttt
     1861 gaaccaaaaa cacattttgc ctcttgaaga aaaggtagag agtaattcgg gttatttggt
     1921 gggttgcact actcagagca atataaggat tgcctgtact tatattttgg atgctaacaa
     1981 agaagggagt tggcaacctc ttgtttataa aggcaaggtt ttttcgttgt ttactgcctg
     2041 cataagatct aatgaagaag ttacgttgac taaacgtatt gtattgaata atagcctttc
     2101 gaatgatgat cccctaactt tagttttagt agaaggcaaa aatttgaaat cgtatgaaga
     2161 tttgcgagaa ctatcggaag tggcttggcg aaatatctgg acgcgggtag atattgaact
     2221 cgaaggtgat cgtttggctc agaaaatttt ccgactgcac ctctttcatt tattcgtaac
     2281 ggcatcacct cataatgctt cgcttgatgc tggtattccc gcacgtgggt tgcatggtga
     2341 agcttaccgt ggacatattt tttgggatga actgttcata cttccgctct ataatttgag
     2401 ttttccagaa atcagccgca gtgccttgat gtatcgttat cgtcggttgc cggctgcacg
     2461 agaatatgcc cgtgcgcatg gctacagagg tgccatgttt ccatggcaaa gcggaagctc
     2521 aggtagagaa gaaactcagg tagtacatct caatcctgtt tctggtgaat ggggcgacga
     2581 ttacagctcc attcagcgcc atgtttcatt ggctatagct tataatatat gggaatatta
     2641 tcattccacc ctcgaccaac aatttctgaa agattatgga gccgaatttt ttttagatat
     2701 ctgcatgttc tggactggct tatgccataa ggatgccgaa gatggaagat accacattcg
     2761 cggtgtaatg ggaccagatg agtttcatga gaaatatcct aatgctaagc gtggaggcct
     2821 cgacgataat gcttattcca acttaatgac agtctgggct ctacaaaagg cctatcaaat
     2881 gagaaatatc attggtgaag aatgttttcg aaacgttgcc agaaaaatcg gtttaaacga
     2941 acaagaatgg caaagatgga aaaatatccg gcaaaatctt gcactttgta ttaatgaaga
     3001 gggtattatc gctcaattcg atggatattt cgaactcaag gaactcgatt gggaatattt
     3061 taagaagaaa tatggcaatg tttatcgcat ggatcgttta ttaaaagccg aaggcttatc
     3121 gcccgatgaa ttcaaggtgg ccaagcaggc tgatgttttg atgatattct ataatctttt
     3181 gcccgaagaa gtgaaccgtt tgcttcaaga aatgggttat gattt
//
LOCUS       DAOORU010000203         3220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_35806_length_3220_cov_7.403791, whole genome shotgun sequence.
ACCESSION   DAOORU010000203 DAOORU010000000
VERSION     DAOORU010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35806_length_3220_cov_7.403791"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3220)
                     /locus_tag="PLF20_09700"
     CDS             complement(<1..>3220)
                     /locus_tag="PLF20_09700"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOX81311.1"
                     /translation="FAVAPANGIVTFPHVWKGNYDIAVVKFPFNSWTLNDDINGNKTY
                     NVLMKEMTFPPKNMTVDDLSLIATWNAARINLVCFEEQWNSGSFATQGWTTSGGSNWG
                     ISTSTGNPAPSAQFNWSPSVTNYSQTLTSPDITGIGSPGLTLEYDYYLNNFSTATLEE
                     LAVEIWNGTTWNQLAHYVNTQGSIPWTHQSININAYTNSTFKIRFRAYGQNCYNINYW
                     NIDNIRVVGSAKAGRDLVGYNVYLNDIQIGFTTDTTYQIPQNLCTYGQTYTACVEAAY
                     ECGVSTNSCYTFTAHYLPAPRNLEATPIQDAAYLTWDYPVVGGKSLQIGIPGMSTESV
                     AEEANDMSTFVAKNINYAPKSTEGILWDNGTIVNSPGTGPGGTDESIIPAGGTSYGWN
                     FNQAVPYSVADDFTVTGNWTITKINFQGYQTGSSTTSTFTGAYFRIYNGNPSAGGTVV
                     WGDLVTNHMTSTEFAGIYRVSAAGGGTDRPVMNIICDGLNINLSAGEYWLEWCTTGTL
                     SSGPWVPNTITPVGNAMQNTGSWVALTNPNPVDLPFVIEGPGGGAAPANLLGYNLYRD
                     GTLAAYVEKSSTEYYDLYLDPGHYCYNITAVYDLEAYGFPAGTTAESLQEGPACVDID
                     YGYPIPWSEDWISSSFTTNHWTLDPEPNHWTVTSINGNPTPSAEFTWAPPATDYSFAL
                     VTPALTSGLFTCADIWLDFEVKLDDRNQTGKESLKVEVLKKGNWTKVAEFINNGSFNW
                     QNQHIDISVIRGKAFKIRFLASGSNSADILDWFVDNINIYPVVYPALNLTAQNNGQNY
                     DVNLQWNSPECPSVATGSEFFEDFEAGVLSPDWEIIQTNTNSTSHPTPAFWSVNNYVG
                     SEFGPFGVYHAGLWWDYSHQDEWLITPEVDIVSDSKLTFESTVWEGSVYGDHYYVKVS
                     TDGGTTWIPVWDASTLTGNGWNYYYYPYSIDLSNFAGKSVKIAFQAIDGDGQGLWYIW
                     FVDNIGLSAGKSTIKFPASSLTYINNGNSSGKMSTQIARDGNTRSVSTEYRAKMRIEN
                     ESLDMDRGVIGYNIYRDGELITPTSVTDTTYKDVVPSNGTYC"
BASE COUNT          849 a          671 c          790 g          910 t
ORIGIN      
        1 tagcaatagg ttccattgga gggtacaaca tctttgtagg ttgtatccgt tacagaagta
       61 ggagtgatga gttcaccatc gcggtagatg ttatacccta taactccacg atccatatcg
      121 agcgattcat tctcgattcg catttttgct ctatattcgg tagaaacact tcttgtgttg
      181 ccatcacgtg caatttgtgt ggacattttc ccactggaat tcccattgtt gatataggta
      241 agagagcttg cgggaaattt aatagtagat tttcctgcag aaaggcctat gttatcaacg
      301 aaccagatgt accacagccc ctgtccatct ccgtctatag cttggaaggc gatttttaca
      361 cttttccctg caaaattgga gagatcgatg ctgtaaggat aatagtagta gttccatcca
      421 tttcctgtaa gagtagaagc atcccacact ggaatccatg ttgtaccacc atcggtggaa
      481 acttttacat aataatggtc tccatagaca gaaccctccc aaacggtaga ttcaaaggtc
      541 aatttcgaat cggaaacaat atctacttca ggagtgatga gccactcatc ctgatgagaa
      601 taatcccacc acaaaccggc atgatatacg ccgaaggggc caaactcgct gcccacataa
      661 ttgtttactg accagaaagc tggagtagga tgagaagtag aattggtatt ggtttgaata
      721 atttcccagt caggagataa tacaccagct tcgaaatctt cgaagaattc acttcctgtg
      781 gcaactgagg ggcattcggg agaattccac tggaggttga catcgtagtt ctggccgttg
      841 ttttgggcgg tgaggttcaa ggcaggataa accacgggat aaatgttaat attatcgacg
      901 aaccaatcca gaatatcggc cgagttggag ccagaggcaa ggaatcttat cttgaaggct
      961 ttaccacgga taacactgat gtcgatgtgt tgattttgcc agttgaacga accattgttg
     1021 ataaattcag ctactttagt ccaatttccc tttttcagta cttcaacttt tagagattct
     1081 ttgccggttt ggttgcgatc gtcgagtttg acctcaaaat cgagccagat gtcggcgcag
     1141 gtgaacagtc cagaggtaag tgcaggagtt accagagcga aggaatagtc ggtagctgga
     1201 ggagcccatg taaattcggc agaaggcgta gggttaccgt ttattgatgt aactgtccaa
     1261 tggttgggtt caggatcgag cgtccaatga ttggtcgtga aggacgagga aatccagtct
     1321 tccgaccagg ggataggata tccatagtcg atatcgacgc aggcagggcc ttcttggagt
     1381 gattcggcag tagtacctgc agggaatcca taggcttcga ggtcatacac tgcggtaatg
     1441 ttgtagcagt aatgacccgg atcgagatag aggtcgtaat actcggtaga agatttttcg
     1501 acgtaagcgg cgagtgtacc atcgcgatag agattatagc cgaggaggtt agcgggagca
     1561 gctccaccgc caggaccttc tatgacgaag ggcaaatcca caggattagg attggttaaa
     1621 gcaacccatg aacccgtatt ttgcattgca tttcctacgg gagtaatggt attgggtacc
     1681 catgggcctg aacttaaagt tccggttgta caccattcta accaatattc tcctgcactc
     1741 aaattgatgt tcagaccatc acaaatgata ttcatcacag gtcgatcggt tcctccaccc
     1801 gcagcactta cccgatagat tcccgcgaat tcggtggaag tcatgtggtt ggttaccagg
     1861 tctccccata ccactgtacc tccggcactg ggattgccat tgtatattct gaaatatgcc
     1921 ccagtaaatg tagaggtggt acttgagcct gtttggtatc cctggaagtt gattttggtg
     1981 atagtccaat tgcctgtaac tgtaaaatca tcggctactg agtaagggac agcctggttg
     2041 aaattccagc cataagaagt tccaccagcc gggattatac tttcgtcagt tccacctggt
     2101 ccagttccag ggctatttac gatagtacca ttatcccata atattccttc ggtagatttt
     2161 ggtgcgtaat tgatattctt ggcgacgaat gtactcatat cattcgcttc ttcggccaca
     2221 gattctgttg acatgcctgg gattccaatt tgaagggatt tgcctcccac aacgggataa
     2281 tcccaggtga ggtaagctgc atcctgaatc ggagtagctt cgaggttgcg aggagcaggc
     2341 agataatgtg ccgtgaaggt atagcagcta ttggtagaaa cgccacattc gtaagcagct
     2401 tctacacatg cagtataggt ttgaccatag gtgcagaggt tttgaggaat ttgataagtg
     2461 gtgtcggtag tgaaaccaat ctggatatcg ttgaggtaaa cgttgtatcc taccagatcc
     2521 ctacccgcct tagcagagcc aactactctg atattgtcta tattccagta gttaatgtta
     2581 tagcaatttt gtccataggc tctgaagcgg attttgaagg tagagtttgt ataggcattg
     2641 atatttatgc tttgatgtgt ccatggaatg cttccctgag tattgacata atgggcgagt
     2701 tgattccatg tagttccatt ccagatttct actgccagtt cttcgagtgt agctgtgctg
     2761 aagttattga gataataatc atactcgaga gtaaggccag gggaacctat tccggtgatg
     2821 tctgggcttg taagggtttg gctgtaattg gttacagagg ggctccagtt gaactgtgca
     2881 ctgggagcgg ggtttcctgt actggtagat ataccccagt tggaacctcc agaggtagtc
     2941 caaccctgag tggcaaagct accgctgttc cattgttctt cgaaacatac caagtttatg
     3001 cgtgctgcat tccatgtggc aatgagggaa agatcatcca cagtcatgtt tttgggtggg
     3061 aaagtcattt ctttcatgag cacattatag gtcttatttc cattgatatc gtcgttgagt
     3121 gtccacgagt taaaggggaa tttcaccact gcgatatcat aatttccttt ccaaacatga
     3181 gggaaagtaa ctattccatt agcaggagcc acggcaaagt
//
LOCUS       DAOORU010000204         3193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_36219_length_3193_cov_5.105163, whole genome shotgun sequence.
ACCESSION   DAOORU010000204 DAOORU010000000
VERSION     DAOORU010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36219_length_3193_cov_5.105163"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>3193
                     /locus_tag="PLF20_09705"
     CDS             <1..>3193
                     /locus_tag="PLF20_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81312.1"
                     /translation="AEVVNYGLPLPVVENPQSNPSDGVLFDNGPFINSPGTGPGGTDE
                     SIIVAGTNSYGFNFNNAIPYTVADDFTVTGTVPWAIGSVEFYGYQTGSTTTSTFTGGY
                     IRIWNGDPSVGGSVIWGDLTTNRMTSTSWINAYRVNVSGGGTTRPIMKVVCSTPGLTL
                     NPGTYWIEFSATGSIASGPWVLPVCLSTPASGNALQFTGTWGPIVSGTAPNTYPQGVP
                     FKLNGAALTGLEYQVWRLKQGDETNPATWTSLGTTATTSIADNSWPTLPNGPYRWAVK
                     AKYTGDRWSDAAFSNVLGKGWTSNVTFNITLSSSTATPQNVMVYMENTSPTADSIYWG
                     LTPASGTINFPHVWKGNYNINIQKFGYQPYTANVDITQNTHTFNILLNETTWAPTDLW
                     VDDRTLIAYWNAPNPQIALFEERWGSGNFTANQWTVNGTNWSVSSYDGNPAPSAEFNY
                     SPQVTNYEQSIVSKDIQGQGSPGLYLKYDIYLNNYSTTNENQMAVEIWDGTAWNRLKN
                     YTNILGSIPWTSESINISSYTWDTFKIRFLAYGVDSWDINYWNIDNIIVAAALSGKSV
                     LGFDMYLDDIQIGFTTDTTYQIPHNLCLYGHSYTASVDAAYESGASDRDYYTFAAHYL
                     PAPRNLEATPIQDAAYLTWDYPVVGSKLEVLDLSPRTSMPNSTYEYSPLVANQQIINA
                     TEAIWDVLFTFNAAGPSRPGVETDGTYIYTSQWNGANFGKFQNVGGTWTLVTEFTIAG
                     ASSIRDLAYDGTYFYGGSASTTIYKMDFNTQTLVGTISTSGVNVRHIAYDPANDGFWT
                     GDWTTMALVSMTGATIQTVSTGLSSMYGDAYDDQSAGGPYLWIFDQGGSGVDIHQMSI
                     ATTTLTGVYHAATDIPGFASGDIAGGLASDNANLVAGKFILLVNIQHDPNIVGAYEIT
                     ASTGGGGAAPANLLGYNVYRDGTLVAYVEKPTTEYYDLYLDPGHYCYDVTAVYDLEAY
                     GFPAGTTAESLEEGPACVDIDYGYPIPWSEDWISSSFTTNHWTLDPEPNHWTVTSING
                     NPTPSAEFTWAPPATDYSFALVTPALTSGLFTCA"
BASE COUNT          843 a          876 c          675 g          799 t
ORIGIN      
        1 tgccgaagtc gtaaattacg gtcttcctct gcctgttgtt gaaaatcctc aatcgaatcc
       61 atcggatgga gttcttttcg ataatggacc ctttatcaat agcccaggaa cggggcctgg
      121 aggaaccgac gaaagtatca tcgtggccgg taccaattcc tatggattca actttaataa
      181 tgcaattcca tataccgttg ccgacgattt caccgttact ggaaccgttc catgggctat
      241 tggttctgta gaattctacg gttatcaaac aggctcaacc actacttcta cctttacagg
      301 gggctatatt agaatttgga atggagatcc aagtgtaggt ggtagcgtaa tttggggtga
      361 tctaaccact aatcgcatga cgtctacttc ttggataaac gcttacagag tgaacgtgag
      421 tggtggaggc acaacccgtc ccatcatgaa agtagtttgc tctaccccag gattaaccct
      481 aaatccggga acctattgga ttgaattttc agctacgggg tctattgcat ccggaccatg
      541 ggttctaccc gtatgcttga gtactcctgc ctcgggaaat gccttgcaat ttactggcac
      601 gtggggcccc atagtaagtg gaaccgcccc aaatacttat cctcagggtg tcccattcaa
      661 attgaatgga gctgctctta ctgggctcga ataccaagtt tggagactca aacaaggcga
      721 tgaaaccaat ccggctacat ggacttccct gggaaccacg gcaacaacca gtattgccga
      781 taatagctgg cctacattac ctaatggccc ctatcgctgg gcagtcaaag ccaaatatac
      841 cggcgaccgc tggtcggatg ccgctttctc caatgttctg ggaaaaggct ggacatccaa
      901 tgtaaccttc aacattacgc ttagctcatc caccgctacc ccgcaaaatg tcatggttta
      961 catggaaaat acttcaccta cggccgactc catttactgg ggacttaccc ctgcatccgg
     1021 aactatcaat ttcccacacg tttggaaagg aaattacaac atcaatatcc agaaattcgg
     1081 ctatcaaccc tataccgcta atgtggatat cacccaaaat acccatacct tcaatatact
     1141 gctcaacgaa acaacatggg caccaactga tctatgggtg gacgacagaa ccctcatagc
     1201 ctattggaac gcacctaatc ctcagattgc cctcttcgaa gaacgctggg gatcgggtaa
     1261 cttcactgct aaccaatgga cagtgaatgg aacgaactgg agcgtaagct cctacgacgg
     1321 gaatccagct ccttctgctg aattcaatta ttcaccccag gttaccaatt acgaacaatc
     1381 cattgtatcg aaggatatcc aaggacaggg ttcacccggt ttgtacctta aatatgatat
     1441 ctatctcaac aactattcaa cgacaaacga aaaccagatg gcggtcgaaa tatgggacgg
     1501 cacagcctgg aaccgcttga agaattatac caatatactc ggaagtattc catggacatc
     1561 ggaaagcatc aatatctcat cctatacatg ggataccttc aagattcgtt tcctggccta
     1621 cggcgttgat agttgggata tcaactactg gaacatcgat aatattatcg tagcggcagc
     1681 cctgagtggg aaaagtgttc tgggattcga tatgtatctg gatgatattc aaattgggtt
     1741 taccaccgat accacctatc agattcctca taacctttgc ctttatggcc attcctatac
     1801 tgccagtgta gatgctgcat acgaaagtgg ggcatccgac agggattatt atactttcgc
     1861 ggcgcattac ctgcctgctc ctcgcaacct cgaagctact ccgattcagg atgcagctta
     1921 cctcacctgg gattatcccg ttgtgggtag caaactcgaa gtactcgacc tttcaccccg
     1981 tacttccatg cctaactcaa cctatgaata cagcccattg gttgccaatc aacaaataat
     2041 caatgctaca gaggctattt gggatgtact cttcactttc aacgcagctg gcccatcccg
     2101 cccaggcgtc gaaaccgacg gaacatacat ctatacttct cagtggaatg gagccaactt
     2161 tggtaaattc caaaacgtag gcggtacatg gaccctcgtt actgaattta caatcgccgg
     2221 tgcaagcagt attcgtgacc tcgcctacga cggtacttac ttctatggcg gctcagcttc
     2281 tactaccatc tacaagatgg acttcaatac ccaaaccctc gtaggtacta tctctacctc
     2341 aggcgtcaac gttagacata tcgcctacga ccccgccaac gacggcttct ggactggaga
     2401 ctggactacc atggctctgg ttagcatgac aggtgctact atacaaaccg ttagcaccgg
     2461 cctcagttct atgtatggag atgcctatga cgaccaaagc gcaggcggtc cttacctctg
     2521 gatattcgac caaggcggca gtggagtcga catccatcaa atgagcatcg ctaccactac
     2581 cctcacagga gtctaccatg ccgctaccga tatcccagga ttcgcttcag gtgatatcgc
     2641 aggtggcctc gcctccgata acgctaacct cgtagccggt aaattcatcc tcctggttaa
     2701 catccaacac gatcccaata tcgtaggcgc ttatgaaata acagccagca ctggcggcgg
     2761 cggtgctgct cccgctaacc tcctcggcta taacgtctac cgcgatggta ccctcgtagc
     2821 ttatgtagaa aaacctacta ccgaatacta cgacctctat ctcgatccgg gtcattactg
     2881 ctatgacgtg accgcagtgt atgacctcga agcctatgga ttccctgcag gtactactgc
     2941 agaatcactc gaagaaggcc ctgcctgcgt cgatatcgac tatggatatc ctatcccctg
     3001 gtcggaagac tggatttcct cgtccttcac gaccaatcat tggacgctcg atcctgaacc
     3061 caaccattgg acagttacat caataaacgg taaccctacg ccttctgccg aatttacatg
     3121 ggctcctcca gctaccgact attccttcgc tctggtaacc cctgcactta cctctggact
     3181 gttcacctgc gcc
//
LOCUS       DAOORU010000205         3164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_36683_length_3164_cov_5.668704, whole genome shotgun sequence.
ACCESSION   DAOORU010000205 DAOORU010000000
VERSION     DAOORU010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36683_length_3164_cov_5.668704"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..868
                     /locus_tag="PLF20_09710"
     CDS             74..868
                     /locus_tag="PLF20_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81313.1"
                     /translation="MMDYKRHLYFSMTPESLISSMLPPEEFGHYLAVGANKRTRGQAL
                     FFEIDPNFQSDYFDLASIERRCVPHVDGQPKRSVYLSTYRVLENLPIEVFSNLYLTTY
                     DGKVLELQPGDYPEEEPFTLHLYQELSPVPPRVASKLNPRQFMNYVTDQRNPVSFPKV
                     VFAELILNGLAENPITGMSNNLPYQNINHLRDCLIGLYSGYEKPNKTVERFYYGDLLY
                     RTVKNGFFLGDQDHFVYYPFPPVDQLQKLYYPWWRSALNLEFNEKK"
     gene            1304..2152
                     /locus_tag="PLF20_09715"
     CDS             1304..2152
                     /locus_tag="PLF20_09715"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOX81314.1"
                     /translation="MKRILVPVDFSDHTFVTASYAFSMAKATGAEILLFHAVFDQFIM
                     PEGYSPDSIITPTMLDVEFIKEIRKEAEEKMAQLFFRLKNQWGEDTVLNAKIESGAPD
                     IAIQAAAEDFQADLIVLGAVGLGRKDHFSGGTAEYLMKHSQKPVLAIPVNCGYKGLNK
                     AIYAVDPAHFHKAELNRIQEFFKPFHAEIHYLVLCYNPKNRPNDSTIAEWQNLVSNRA
                     SFQIVDCDHPQEGLHLSIRNIQPDIIAFHFFRESLFSRWFKPALSRKDLYKANLPLLS
                     FPEIEE"
     gene            2149..2655
                     /locus_tag="PLF20_09720"
     CDS             2149..2655
                     /locus_tag="PLF20_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOX81315.1"
                     /translation="MIQIGLLSDTHGVLLPFVIKALDHVDEIWHAGDIGHVEVYEQLR
                     KMKPLRAVHGNIDSWTLRCQCPEYLSFMIEDVKVLMIHKAGSPGKYSPACRKLIISEK
                     PGLLIGGHSHILKVMQDNSNNLLFLNPGAAGLEGIHKVMTLLRFSLDKGKVVNMEIIE
                     KERSPSRI"
     gene            complement(3047..>3164)
                     /locus_tag="PLF20_09725"
     CDS             complement(3047..>3164)
                     /locus_tag="PLF20_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008823763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOX81316.1"
                     /translation="FAGEIIDLDADTGGYNLQIAFSTGWIAGESAAIQNVDE"
BASE COUNT          922 a          611 c          591 g         1040 t
ORIGIN      
        1 ggactggatt tttattcgtc tgtgtgtcaa tcgaaagttt tcatgtaaat ttgtcgaacg
       61 taaatagaaa attatgatgg actataaaag acacctatat ttttcgatga ctcccgagtc
      121 attgatttct tcgatgcttc cgcccgagga atttgggcat tatcttgctg ttggcgccaa
      181 taagagaaca cgtgggcagg cattgttttt cgagattgat cctaattttc aatccgatta
      241 ttttgatttg gccagtatag aacgacgttg tgtccctcat gtcgatggac agcccaaacg
      301 ttcggtctat ctctcgactt atagagtact cgaaaattta cctatcgagg ttttcagcaa
      361 cctctatctt actacctatg acgggaaggt tttagaactt cagcctggag attaccctga
      421 agaagaacct ttcacgcttc atctttatca ggaattgtct ccggtacctc ctcgtgtagc
      481 cagcaagcta aatcccaggc aattcatgaa ttatgtaact gatcagagga atccagtctc
      541 tttcccaaaa gtggtatttg ctgagcttat tctcaatgga ttggccgaaa atcctatcac
      601 tggcatgtcg aacaatctcc cttatcaaaa tatcaatcat ttacgcgact gcctcatcgg
      661 tttatattca ggctacgaaa aacccaacaa aaccgtcgaa cgtttttatt atggagattt
      721 gctctatcgt accgttaaaa atggattttt tcttggtgat caggatcatt tcgtatatta
      781 tcctttccct ccggtcgatc aattacaaaa actttattat ccgtggtggc gttcggccct
      841 taatctcgaa tttaacgaga aaaaatagat tgatatattg aaattcatta attctctctg
      901 cattccatca agcaaggatg aatatattaa cgattttatc gaattaccaa tgatgctgtt
      961 gtcttatatt cattttcgta tgaaaaacca tgcattgaaa aggctttttt atcatatata
     1021 aaaaataaaa atcatacgac ttttggattg cgatttttta ttgtgcctta tttgtaatga
     1081 ttagaattat tgtattgcat ttattttttt gagtatcgaa aatccgacaa attaaatagt
     1141 ttcagaattt atcaatattt ttttgttgat aaagttttat tttcgaaaaa attaagggtt
     1201 tcttggatat tgccatataa ttttttacca agttcacgca atggattcct ttttggttta
     1261 tatttgtaga agtttttcaa tcattcgaaa atacctttga gatatgaaac gtattcttgt
     1321 tcctgttgat ttttccgacc atacttttgt tacagcctca tatgccttct cgatggcaaa
     1381 ggctacggga gccgaaattt tgttgtttca tgcggttttc gatcaattta tcatgcctga
     1441 ggggtattct cccgattcta ttattactcc caccatgtta gatgtagaat ttataaagga
     1501 aattcgaaag gaagcggaag aaaaaatggc acaattgttt tttcggctaa aaaatcaatg
     1561 gggagaggat actgtattga atgcgaagat agagtcgggt gcacccgata tagctattca
     1621 agctgctgcc gaagattttc aggccgacct cattgtttta ggagcagttg gtttgggacg
     1681 taaagaccat ttttcgggag gtacggccga atatcttatg aaacattctc aaaaacccgt
     1741 tttggccatt ccagttaatt gtggttacaa aggattgaac aaagcaatct atgctgtcga
     1801 cccggcacat tttcataaag ctgaactcaa ccgaattcag gaattcttca aaccatttca
     1861 tgccgaaatc cattatcttg tattgtgtta taatcccaag aatcgtccta atgactctac
     1921 catagctgag tggcaaaatt tagtgagtaa tcgggcttcg ttccagatcg tcgattgcga
     1981 tcatcctcag gaaggtcttc atctttctat tcgaaacata caacccgaca tcattgcatt
     2041 tcattttttc agagaaagcc tcttttcacg ctggtttaaa ccagccctct cgcgtaaaga
     2101 tctatataag gcaaatcttc cgcttttgtc attccccgaa attgaagaat gatacaaata
     2161 ggtttacttt ccgatacgca tggtgtttta ttgccatttg taatcaaagc tctcgatcat
     2221 gtagatgaaa tttggcatgc cggcgatatt ggtcacgtag aggtttatga acagcttcga
     2281 aaaatgaagc ctttaagggc agttcatgga aatattgata gttggactct tcgttgtcaa
     2341 tgccccgaat atctttcatt tatgattgag gatgtaaaag tattaatgat acacaaagct
     2401 ggaagtccag gaaagtattc tcctgcatgc cgtaaactta ttatttcgga gaaacctggt
     2461 ttgctgattg gtggtcactc tcacatcttg aaagtaatgc aggataattc gaacaattta
     2521 ctattcctta atcctggtgc tgcaggactc gaggggatcc ataaagtaat gactctgctc
     2581 agattttcct tggataaagg gaaggttgtg aacatggaaa ttatcgaaaa agaacgatct
     2641 ccctcgagaa tataatctta tgagctaaga taaaaaataa ggaatcaatc ggtgttttat
     2701 gggttgaagt gaaaccgaca aagtcaggaa agcatgacct gaacactttc ctcacacttg
     2761 catagcccag atttcaccta cgttattgcc tcgataccaa atatatattc attaaagtca
     2821 atgcttgcta aatcctattt atacgagaaa gacttgagtt cgacatcaga gaaatccgaa
     2881 ctttcacctc cctatgatcg ttaacttaaa taacagattt ttcctgagac caccatcatt
     2941 tgttcttata gattgcttcc attgggcaaa ttaaagtaaa cttgagcctt tactcgctta
     3001 gttataattt ccggctcacc taactatgcc gatactgatt cctatattat tcgtcaacgt
     3061 tttgaattgc ggccgattct cctgcaatcc aaccggtgga gaaggcaatt tgaagattgt
     3121 atccaccggt atctgcatca aggtcaatta tttcgcctgc gaag
//
LOCUS       DAOORU010000206         3151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_36894_length_3151_cov_4.704780, whole genome shotgun sequence.
ACCESSION   DAOORU010000206 DAOORU010000000
VERSION     DAOORU010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36894_length_3151_cov_4.704780"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1807
                     /locus_tag="PLF20_09730"
     CDS             <1..1807
                     /locus_tag="PLF20_09730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HOX81317.1"
                     /translation="NPIGMAALNDHTLNEDNFLGKTFATVKFSEKLEWNTNFGIDYGN
                     NFTRWFNPTWGTFNRINAKNNLSIVSQRATNWTLNSVLNYETQFGENHHFSAMGGFEA
                     IHNSGRYLNASDSDFPVQESNLIYIGNGQGTKTVSESEYASSLLSFFGRVSYDFKGKY
                     YLSGTLRRDGTSRFTGDNQWGNFYSLSAGWLLKQEAFLKNVDWLENLKLRLGYGAIGN
                     QEIGLYAYSDRISPYYNYPFGEIVSSGYAQTALGNEKLKWETSYQYNAGIDAEFWKGA
                     LAISLDYFYKITDDMLVKASYPPSAGYASSPWINNGSVLNTGLELEASYRKAGNEWSY
                     NIGGNLTYLHNEVRKLNAPLIGGRVESGVYATYTTEGQPIGAFYLLEMDGIFQNETEI
                     ILSAFQGNNIHPGDVKFVDQNKDGKITNEDRVFLGSAIPKFSGGISFNGQWRQWDMGI
                     FLQGVAGNKIYIQINQDIEGFYRGFPVTQRYFDNRWTGEGTSNTQPRPSWTAKSNNAR
                     PSSRFLEDGSYLRLKNIQIGYTLPKETLNFLGFTKARIYLSASNLFTITSYPGLDPEM
                     TVSDNSKNEGDRASGIDWGTYPSTMSIMLGLDLSI"
     gene            1822..>3151
                     /locus_tag="PLF20_09735"
     CDS             1822..>3151
                     /locus_tag="PLF20_09735"
                     /inference="COORDINATES: protein motif:HMM:NF025680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOX81318.1"
                     /translation="MKININQFKHIGSGLLLIATLSLLPISCTKDFLDEELKGEFSSQ
                     TFYQNDKQALQALTGVYNAIAFTSFNNAIWVFGDVASDDAVKGGNPGDQAEITYIDEF
                     TVDRNNGIINNYWTFLYEGVSRANNVINKVPGVKMDETLKQRIIGEAKFIRAYNYFLL
                     VNIFGKVPLKLLPQDTPEAINVPLSEISTIYQQIQTDLTEAAAVLPVSYGSSEKGRVS
                     KGAALAMLGKAALYQQQWALAIDYFHQLEALGIYGLETNYANLFKLSHENGVESIFEI
                     QHLSDQIPFIGNVLNQWFAPASEGGYYFNAPTTSLVNEFEKASNQTPDPRLDISIGRD
                     GIPWLNGEPFQASWSPTGYLTKKHQQPLSEVPIAVKGDGGLNYIYMRYADVLLMKAEA
                     FNELNQTDSALANLNKVRARARNSFTSGNPPADLLPNVTTTDQHLLRNAIR"
BASE COUNT          939 a          678 c          672 g          862 t
ORIGIN      
        1 taatcctatt ggcatggcag cccttaatga ccatacactt aacgaagaca attttttagg
       61 aaaaaccttc gctacggtga agttctcgga aaaactcgaa tggaatacca attttgggat
      121 agattatggc aataacttta cacggtggtt taatcctacc tggggtacat tcaaccgaat
      181 caacgccaaa aataatctaa gtatcgtaag tcagcgtgct actaattgga cgctgaacag
      241 tgtgttgaat tatgaaactc aatttggtga aaatcatcat tttagtgcta tgggtgggtt
      301 cgaagctatt cataattcgg gaagatatct caatgccagc gattctgatt tcccagttca
      361 ggaatcgaac ctgatttata tagggaatgg acaaggcacc aaaacggttt cggagagtga
      421 atatgcttct tcccttctct catttttcgg acgagtaagt tatgatttca aggggaaata
      481 ttatttatcg ggtacgcttc gtagagatgg aacctcgcgt tttacaggcg acaatcaatg
      541 gggaaatttc tatagcctct cggcagggtg gcttctcaaa caggaagcct tcctgaagaa
      601 cgtggattgg ctcgaaaacc tgaaactaag gctgggctat ggagccatag gcaatcaaga
      661 aatcggcctt tatgcctatt ccgacaggat atcaccctat tataattatc ctttcggtga
      721 aatcgtaagt agcggctatg ctcaaaccgc tctgggaaat gaaaaactga aatgggaaac
      781 gagttatcaa tataatgctg gcatagatgc cgaattctgg aaaggtgctc tggcaatttc
      841 actggattat ttctataaaa ttaccgatga tatgcttgta aaggcgtctt atcctccttc
      901 ggcgggttat gcctcttccc cttggattaa taatggttcg gtattgaaca ccggcctcga
      961 actcgaagcc agttatcgca aagccggcaa cgaatggagc tacaatatag gcggtaatct
     1021 tacttatttg cataatgaag taagaaaact gaatgcccct ctgattggtg gtagagtcga
     1081 atcgggtgta tacgctacct atactactga aggacaaccc attggagctt tttatcttct
     1141 cgaaatggac ggtattttcc aaaatgaaac cgagataatt ctttcggctt ttcaaggaaa
     1201 caatatccac cccggtgatg tgaagtttgt agatcagaat aaagatggaa agattaccaa
     1261 cgaagacaga gtatttttag gcagtgctat tcccaaattc agtggcggca ttagctttaa
     1321 tggccaatgg cgtcaatggg acatgggaat atttctgcag ggcgtagccg gaaacaaaat
     1381 atacattcaa atcaatcagg atatcgaggg cttttatcgt ggcttccctg ttactcagcg
     1441 ttatttcgac aatcgctgga cgggcgaggg gacttcgaat actcaacccc gtccgtcatg
     1501 gactgcaaaa tcgaataatg cacgaccttc ctcacgtttc ctcgaagacg gttcctatct
     1561 ccgattgaaa aatattcaga ttggatacac ccttcccaaa gaaacactta acttcttagg
     1621 ctttaccaaa gcaagaatct atctttcggc aagcaatctt ttcaccatta cctcctatcc
     1681 tggcctcgac ccggaaatga ccgtcagcga caactcgaaa aatgaaggtg acagagccag
     1741 tggtatagat tggggaactt atccttcgac aatgagtatt atgttaggtt tagacctttc
     1801 tatctaaaaa attgaaatat tatgaaaatc aatataaatc aattcaaaca tatcggtagc
     1861 ggacttctgt taatagccac cttgagcctt cttcccatat cctgcacaaa agattttctt
     1921 gatgaagaac tgaaaggaga atttagcagc cagacattct atcagaatga caaacaagcc
     1981 ttgcaggctc tcaccggtgt ttacaatgcc attgccttta ccagcttcaa taatgcgata
     2041 tgggtctttg gagatgtggc ctcggatgat gccgtaaaag gaggcaatcc tggcgatcag
     2101 gcagaaatta cctatataga cgaatttacc gtcgaccgta ataacggcat catcaataat
     2161 tactggacat ttttatacga aggggtatcg agagctaata atgtaatcaa taaagtaccc
     2221 ggcgtaaaaa tggatgaaac cctcaaacaa cgtatcatcg gcgaagcgaa gttcatcagg
     2281 gcttataatt atttcttact ggtcaacatc tttggaaagg tacccttaaa acttttgcct
     2341 caggatactc ctgaagccat caatgtacct ctgtcagaga ttagtactat ttatcagcaa
     2401 attcagaccg atttaactga ggcagcagcc gttctgcctg tttcgtatgg cagttccgag
     2461 aaaggcagag taagcaaggg agccgctctg gccatgttag gcaaggcagc actgtatcaa
     2521 caacagtggg ctctggcaat agattatttc catcaactcg aggccttagg gatttatgga
     2581 ctcgagacca attatgccaa tcttttcaaa ctatcccacg aaaatggtgt agaatccatt
     2641 tttgagatac aacatctatc tgaccagata ccttttatcg gaaatgttct caatcaatgg
     2701 tttgcacctg catcagaagg aggttattat ttcaatgcgc ctaccacttc gctggtgaat
     2761 gaattcgaga aggcttccaa tcaaactccc gatccaaggc tcgacataag tattggccgc
     2821 gatggaattc catggcttaa cggagaacca ttccaggctt catggtctcc cacgggttat
     2881 ttaaccaaaa aacatcagca acctctgtcg gaagttccta ttgcggtaaa aggcgatggt
     2941 ggccttaatt acatttacat gagatatgcc gatgttttat tgatgaaagc cgaggctttc
     3001 aatgaattga atcaaaccga ttcggctctg gctaacctga acaaggtacg tgcaagagcg
     3061 cggaacagtt ttaccagcgg caatcctcct gccgatcttc ttcccaatgt gactactacc
     3121 gatcaacatc tgcttcgtaa cgccattcgc c
//
LOCUS       DAOORU010000207         3148 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_36941_length_3148_cov_6.226641, whole genome shotgun sequence.
ACCESSION   DAOORU010000207 DAOORU010000000
VERSION     DAOORU010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36941_length_3148_cov_6.226641"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(329..2272)
                     /gene="yidC"
                     /locus_tag="PLF20_09740"
     CDS             complement(329..2272)
                     /gene="yidC"
                     /locus_tag="PLF20_09740"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOX81319.1"
                     /translation="MDKNTIIGLVLIFAIFIAWSIWMSPSKEDVEKQKRIHDSLVMVQ
                     MKKDSIAQALEKQKEENKTALPIATQKISSPQQADSLLRIAREKVGQFAGSVIGETKY
                     YTLENDLVKIKISNLGAQPFKVELKNFKTFDSLPLILFDSLQSNFGLNFFAGRRQINT
                     AVLFFEPEPGNESRYHYKVSGDSTISFALRLYADSPDSLSPLNSYIEYKYTLKGNEYM
                     MGFSMSMVNMDKVFDRTTSLFNLNWNMDWLRQEKNRDDERRQSTVYYCYSTDKEVKNL
                     NEAKDDKKIITTSLKWVSLKSKFFLATLIAENAFPNGAEISTYIDPERANEPRYNKSM
                     SVQIPLEYGFSERMDYPMQIYFGPNQYKILHAYNLRLERQIPLGWGFFLMWPVNVYAI
                     IPVFNWLSNYGVNYGIIILILTVLLKIVLFPITFVSYRSTAKMRALKPEIDEINKKYQ
                     RKEDAVKKQQATMDLYKKAGVNPMSGCLPMLLQFPILIAMFRFFPSSIELRQQPFLWA
                     HDLSSYDSILDLPFKIPFYGDHVSLFTLLMTIATLFYTKINNDLMNTGQQQIPGMKLM
                     TYLMPILFLGIFNNYSAGLSYYLLVAQLITFGQMFIFRKAINEEKLRARLLANKNKPV
                     KKSNFQKRLEEMARQQQQQARRK"
     gene            2637..2969
                     /locus_tag="PLF20_09745"
     CDS             2637..2969
                     /locus_tag="PLF20_09745"
                     /inference="COORDINATES: protein motif:HMM:NF007495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HOX81320.1"
                     /translation="MISWDDFQKLDVRVGKIIEAEIFPEAKKPSYKLKIDVGPQIGIK
                     KSCAQLTVNYSLKDLIGKLVLCVVNIQARQIGHFISEVLTLGVPDIKGECILITPDRN
                     VPLGSRLY"
BASE COUNT          970 a          574 c          605 g          999 t
ORIGIN      
        1 ttgatgaaaa gttttggaaa aatttttagg gtgacgcatt gtcgaagtca ggagacgagg
       61 agactaaaag aatacccatg tcgaatatca tgaattattt tttaaatgat aacatatcca
      121 ttttcagtct tttaattaat acaccaatcc ctatccccca aacccttcca ccatttctcc
      181 ctttctgctt tatagatttt caacttttta ctcaccaatc cccctccaaa tgccattctc
      241 cgttcatcgt ctttatttat gctgtaactt ctcaaacatg tcgcacatcg cgaaacggag
      301 gatcgcccct gcttgtcatt tatgtaaatt atttccttct tgcctgttgt tgttgctggc
      361 gagccatctc ttcgaggcgt ttctgaaaat tcgacttttt aaccggttta ttcttattgg
      421 ccagcagtct tgctctcaat ttttcttcat tgatagcttt gcggaagata aacatctgtc
      481 caaaggtgat aagttgtgcc acaagtaaat aataactcaa accggcggag taattattga
      541 aaatgccgag gaataaaata ggcattaaat aggtcataag tttcatgccc ggtatttgtt
      601 gttgcccggt attcattaag tcgttattga tttttgtata gaagagtgtt gcaatagtca
      661 tcagtaatgt aaagaggctg acatggtcgc catagaaagg tatcttgaaa ggcaggtcga
      721 ggatagaatc ataactggaa aggtcgtgag cccagagaaa gggttgttgc ctgagctcga
      781 tggaagaagg gaaaaacctg aacatggcaa taagaatagg gaattgtaac agcatgggca
      841 aacagcccga catggggttt actccggcct ttttatacaa atccatagtt gcctgctgtt
      901 ttttcacggc atcttccttg cgttgatatt tcttgttgat ttcatcgatt tctggcttca
      961 acgcacgcat tttcgccgta gaacggtaag aaacaaaagt gatgggaaat aagactatct
     1021 tcagcaaaac ggttaaaata aggataataa tcccgtagtt tacaccgtaa ttgctcaacc
     1081 agttaaatac aggaataatt gcataaacat ttacaggcca cataagaaag aagccccatc
     1141 ctaatggaat ttgcctttct aatcgtaaat tataggcatg taaaatttta tattggttgg
     1201 ggccaaagta aatctgcatc ggataatcca tcctctcaga aaagccgtat tctaaaggaa
     1261 tttgaacgga cattgatttg ttatatctag gttcgttagc ccgctcggga tcgatatagg
     1321 tagaaatctc ggcaccatta gggaatgcat tttcagcaat gagtgtggca agaaaaaatt
     1381 tcgatttcaa tgaaacccat ttcaagctag tagtaatgat ttttttatcg tctttggctt
     1441 cattgaggtt ttttacctcc ttgtcggtgg aatagcagta ataaacggta gattgtctgc
     1501 gctcgtcatc tcgatttttc tcctggcgca gccaatccat attccagttg agattgaaaa
     1561 gagaagtggt tcggtcgaaa accttatcca tattgaccat gctcatgcta aaacccatca
     1621 tgtattcatt cccttttagc gtatatttat attcaatata agaattcaaa ggggaaaggg
     1681 aatcgggcga atcggcatag agccgcaatg caaatgagat tgtagaatct ccgctcactt
     1741 tatagtgata tcgactttcg ttgccgggtt caggttcgaa gaagagaact gccgtattga
     1801 tttgtcttct gcctgcaaaa aagttcaatc caaaattcga ttgtaaacta tcgaaaagaa
     1861 taagtggtaa cgaatcgaaa gtcttaaaat tcttcagttc gaccttgaag ggttgagctc
     1921 caaggttgga tattttaatt ttaaccagat cattttcaag agtataatat tttgtttcgc
     1981 caattaccga gcctgcaaac tgacccactt tttctcgagc aattcgaagc aaagagtcgg
     2041 cttgttgagg agatgaaatc ttttgagtag caattggcag cgctgtttta ttctcctctt
     2101 tttgcttttc gagcgcctga gcaatggaat cttttttcat ctgaaccata accaacgaat
     2161 catgtatccg cttttgcttt tcgacatcct ccttggaagg actcatccag atgctccatg
     2221 caatgaaaat agcaaaaatg agtactaagc caataatagt gtttttatcc atttattagt
     2281 aaatacatgt gtataaaggg tatcgggcta ttccccggat aaaggctgca aagataaagg
     2341 atttttttgt aggacatcta taggaagaat tcttgcatat caatttccat tatattaata
     2401 tatagatgat tatgaattca catggctgtc ggcaagctta taaaaaatat tcataggaat
     2461 tccaatctct ttactgatca ttaaatataa tccacagttt cttcagtttc aaaggcaatt
     2521 gatcttgaaa aattcagaga aaacttttac ttttgcgcta tgtccagcta ttcatttcca
     2581 aagattaaaa aaaagtaaga acgagcgaaa acaaaaatta ttataaattt aattttatga
     2641 tttcatggga tgattttcaa aaattggatg ttcgtgttgg gaaaattatt gaagccgaaa
     2701 tttttcccga agcaaaaaaa ccgtcttata aactcaaaat tgatgtgggt cctcagattg
     2761 gaataaagaa gtcgtgtgcc caattgacgg taaactactc attgaaagac ctcataggaa
     2821 agctggtgtt atgtgtggta aacatacaag ctcggcaaat cgggcatttc atttcagaag
     2881 tattgacatt aggcgttccc gatataaaag gagagtgcat tctcattact ccagatagga
     2941 atgttccttt gggttcgagg ctttactaaa tgaagctgat taattacgtt atttggaata
     3001 gaaattcatt tttttatttt tctgaaaata aatcacttaa acactataat cacccttcat
     3061 ggagtctgaa gaaggtgggt ttatgtcgaa attattaata atccatccta agaccggctc
     3121 aatcagaaat tttatcctgt ttgcataa
//
LOCUS       DAOORU010000208         3126 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_37291_length_3126_cov_3.873331, whole genome shotgun sequence.
ACCESSION   DAOORU010000208 DAOORU010000000
VERSION     DAOORU010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37291_length_3126_cov_3.873331"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PLF20_09750"
     CDS             <1..536
                     /locus_tag="PLF20_09750"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOX81321.1"
                     /translation="LYLGSAFEVVYYSEDKTGPVEIIELWKGVTPSAALIFNTGKYMK
                     NHWKSAGVSIYKGYVSLWLGESKDPETKIRLCSSDSVINSVVSKPSSKSNITREDISQ
                     ASLGQYYLIFGSYPNFQLARQAQIDLINDGFKNAFIFEKEGKFRTALDKYETMEAARA
                     AKKQLPNKYKNAWILKE"
     gene            653..794
                     /locus_tag="PLF20_09755"
                     /pseudo
     CDS             653..794
                     /locus_tag="PLF20_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018110133.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1067..2389
                     /locus_tag="PLF20_09760"
     CDS             1067..2389
                     /locus_tag="PLF20_09760"
                     /inference="COORDINATES: protein motif:HMM:NF017396.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HOX81322.1"
                     /translation="MLHRLILSITLPILMVLMLSYKPKPLSPEEVLSSIPLVDSFRVI
                     LSDKLFSETYEVWFRMPLDHSNPNGLSFPLRVYYSHNGFDKPMVVVVDGYTMYTSKAN
                     EVTCIVDGNQITIEHRFFSKSRPMDSIPWSYLNIRQAAADAHLVISAFKPFYHGKWIS
                     TGISKSGQATIYHRRFYPTDVNVSIPYVAPLNFSSEDERVYKFLDSVGTPECRERIHD
                     FQVQLFERKNEILPLFEELAASKGWNFTMGLERAYILSILEYSFSFWQWGFSCSSIPA
                     SSSTAKELFKHWLDVNPFTFFEENEVDKVRPFFYQAMTEIGMYGYRVKPFSRYLSDTT
                     NITFSFTMPKGHQAVFNASTMRDIDRWIKDSGNYMLYIYGQNDAWSATAVDPGGKTNA
                     VKMVCPGGSHSSRIKDFPPELQDSIYRVLGRWLDLRLPANGDIKLNPD"
BASE COUNT          917 a          628 c          618 g          963 t
ORIGIN      
        1 ccctatatct tgggtcagct ttcgaagtag tttattattc cgaagacaaa actggtccgg
       61 ttgaaattat tgaactatgg aaaggagtaa ctccttctgc tgctctgata ttcaatactg
      121 gaaaatacat gaagaaccac tggaaatcgg ctggtgtgtc tatatataag ggttatgtct
      181 ctctctggct tggagaaagc aaagaccctg aaacaaagat acgcctttgc tcttctgatt
      241 cagttattaa ttctgttgtt tcgaaacctt ccagtaaatc gaatattacc cgtgaagata
      301 tttctcaggc ttctctaggt caatattatc tcatatttgg cagttacccg aattttcaac
      361 tagctcgtca agcccagatt gatttgataa atgatggatt caaaaatgcg tttattttcg
      421 aaaaagaagg aaagtttaga actgctttag ataaatacga aaccatggaa gcagctcgtg
      481 cagccaaaaa gcaattgcct aataaataca aaaacgcctg gattttgaaa gagtaataag
      541 aaaatccccc aacgaaaagc tctcgattag atcagaaagt tggactgaaa tatccataat
      601 gtgagatcga cctagtcgat aagggaggat gggggagggg gttttgctca tacatcttct
      661 cgaaacagga acagatatca ggtatattca gcaactactc gggcataact ccttgaaaac
      721 caccgaaatc tatatatata ttaccgacat tgccaaatca tacattcatt aaaagtccac
      781 ttaatacgtt atgaattgaa ttataaatat tctatcattt gttaagcata ggtggcattc
      841 agcgaacttc gccgagtggg gaaaactccc ttcgatggca ctgcaggtaa agatgtgctt
      901 agggtgccga acatacttaa caaccgtctc ctccaaaaaa actcaacttt gtatcttcga
      961 atgaattttt attacgtaat tcgtctaacc aaaaagttgc attaattact tgaatttagg
     1021 catgaataat atgaaacgaa aacaacgaca acaaaagtgc ttgaagatgt tacacaggct
     1081 aattttaagc attaccctgc cgattttgat ggtgcttatg ctttcgtaca aacctaaacc
     1141 cttatcgccg gaagaagtgc tttcgtccat accgctggta gacagtttca gggtaatcct
     1201 atccgacaaa ctattttcgg aaacctacga ggtgtggttt agaatgcctc ttgaccactc
     1261 caatcccaat ggtctatcat ttcccttacg ggtttactat tctcataatg gttttgacaa
     1321 accaatggtt gtcgttgttg acggctatac catgtacacc tccaaggcta acgaggtaac
     1381 ctgcattgtt gacggaaacc agataaccat cgagcatcgt tttttttcga agagcaggcc
     1441 aatggactcc attccatgga gctacctcaa cattaggcag gcagcggctg atgcgcattt
     1501 ggtaatatct gcatttaaac ctttctacca tgggaagtgg atttcaacgg gtattagcaa
     1561 gagcgggcag gccacaattt atcaccggcg tttctatccc accgatgtta acgttagtat
     1621 accctacgtg gccccactga acttttcaag cgaagacgaa agggtgtaca aatttcttga
     1681 tagtgttggg acgcctgaat gccgtgaaag gatacacgat tttcaggtgc aacttttcga
     1741 aaggaaaaat gaaattcttc cacttttcga ggagcttgca gcaagcaagg ggtggaattt
     1801 cacgatggga ctcgaaaggg cctatatcct gagcattctg gagtactctt tttcattctg
     1861 gcagtgggga tttagctgct cgtccattcc cgcaagtagt tcaaccgcaa aggagctttt
     1921 taagcattgg ctcgatgtta acccctttac ctttttcgaa gagaatgaag tggacaaggt
     1981 tcgccctttc ttttaccaag ctatgaccga gattggaatg tacggctaca gggtgaaacc
     2041 gttcagtcgc tacctcagcg ataccaccaa tataaccttt agttttacta tgccaaaggg
     2101 gcatcaagcg gttttcaacg cctccacaat gcgcgacatt gatcgttgga ttaaggattc
     2161 gggtaattac atgctctaca tatacggaca aaacgatgca tggtcggcta cggctgttga
     2221 tccaggggga aagactaatg cggtgaaaat ggtttgtccc ggcggttcgc actcgtcaag
     2281 gattaaggat tttcctcccg aacttcagga tagcatttac agagtcctgg gcagatggct
     2341 cgaccttaga ttgccggcta atggcgacat taaactaaac cctgattagt aatcatttat
     2401 catgatgaaa tttcagaacc cctgatcctt ttattgcaaa tcgatttttt acaaaagttt
     2461 cctcatactt caaattatcg tattcttgag cagatatgca actttaatta tctatttttt
     2521 ctaccgttag cgataggttt attcattcct tgtcaaatct ttacacatta tgtaagtgcc
     2581 aacaaatccc aagtccttga tgtcttgggt caaccatata actgcaattt gcgatgggat
     2641 tgcctaagtc gatttggttt ggcttaataa tataggttcc caaaattccg tcattatcaa
     2701 taaaaacaaa ggttaatata tagtcaacac accaatgctt atgcaaactc tctttgggag
     2761 tattggggtc aaatatataa gtgtcgccta tcgcaattat atttttggaa atgttccaaa
     2821 ctttgtcgaa gtcggtttat ttggcacttc gtatgatttc caaataaaat tcaacgtttg
     2881 ttgttgtccg ttactttctg tttcggggtc tgtaaaaatt atcgctaatg gtttttgcta
     2941 tggtgcgtgc cgtattttta cttctatgtt ttttgatttg tactattttc aaattttcaa
     3001 ttttctttct aaatttcttc aacctgagga atgcactata atccactgta ataggatttt
     3061 tgttattaag ttagttataa tatcaacaga aatctttcca gttttaaata gagtctgtct
     3121 ataaac
//
LOCUS       DAOORU010000209         3119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_37393_length_3119_cov_5.531984, whole genome shotgun sequence.
ACCESSION   DAOORU010000209 DAOORU010000000
VERSION     DAOORU010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37393_length_3119_cov_5.531984"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(352..3000)
                     /locus_tag="PLF20_09765"
     CDS             complement(352..3000)
                     /locus_tag="PLF20_09765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX81323.1"
                     /translation="MMKTYKFWLCAFVLLILQTQGLNAQVSNPRNAAHDFSSGSTSSS
                     IQSKELVNYAGEGGLSNVISFCSSELSQLPNKEIMFDENGNLPYTMYGKKIFHNNYSQ
                     LELPNANQDAGYPWNSIGPWGGDVTSLASSFETPATLYAAAGLPFIKYDTSSSWILWE
                     SLAAYTTGVNLIATAPDGIIYVSGGNTWNFFKSNDGGINWTQLTIPINLRDISAISID
                     PTNSDIVYLGLASTLGATNWLQILKTTDGGSSWQILNTSNLNVDLGVADIAVWPDNPS
                     QLVAAAYGAFGGGEVIYSNNSGETWQTVTNNLPNSYPFNDIEIAGHTVYLGGGQLFSS
                     QHLGLYTCKLGEFAWQNISSNFPLKVVAKVLVSPVDSLKIYAGTDGDGVYFSTDGGLN
                     WDYSSTGASNFSITDMIFYPNNPEEILIGCKSLAVYRSQNAGINWENYSNGIATLPIN
                     DIAVDPLDCNNIIVAFEALNSGGCYMSNDGGNTWNPVSGLPATRFSAVTYDIHGYVYA
                     VSRGPSSVAQEGIYKSTDGGITWTNTGPNIGPLFETELWTIEQTPDQPGLLFIGGNHF
                     GVAGYAATIYRTLDAGQSGWEEVYIGADQMKIHALEIAPFSDEQLIYAGYVVYGGNGS
                     LLKSTDQGNHWVEINNGLPNNCCQTYAIAVDPSNPQKVFAGSGYYAAGYYIVKTEDGG
                     ENWSPIISLPSQVNCLASIEIPIIKNREKISLDGDPRIIYAGTSSNGVFLSDDDGMSW
                     VDASEGLPANTYVSNFSDPFLTGVELKINASTLGHSGYSTTIAILEGINELSKEDVLT
                     WNEPNPFSQHTTIHFTLKKNSMVRLSIVNMNGQVIATLLDQKMSSGEHQFIWNAASHG
                     KKLARGLYFYRLEVDGKNYFNKLIVQ"
BASE COUNT          946 a          656 c          599 g          918 t
ORIGIN      
        1 tatccaacca gtaaggaact ttttcttttc cgaactgata ggctaaagct tcatgaaata
       61 atggctcatc gataattttg gaaagatagg aatgcacttc ctcatctcta aatgagcgta
      121 tgggatcgaa tacattttgt ttcatgcggc gaaattaata agattattga aacacatcat
      181 catttgattt gagtcggcct tcaaaatcca cgccagaatg agtaatgaca gagaattcaa
      241 tagtgaaata atttctctga aaattttaaa aaagatattc ctccattcaa attcgaaaag
      301 gggagactaa gctccccctg aatgttccat tatgtatcgt atcaaatatt tttactgtac
      361 gattaattta ttgaaataat ttttaccatc tacttcaagg cgatagaaat aaagtcctcg
      421 cgctaatttt ttaccatggc tcgcagcatt ccatataaac tggtgttctc ccgagctcat
      481 cttttgatcg aggagggtgg caattacttg accattcata tttacgatag acagacgcac
      541 cattgagttc tttttcagtg taaaatgaat ggttgtgtgt tgagaaaatg gattgggttc
      601 gttccaggta agcacatcct ctttgcttaa ttcattgatg ccttccagaa ttgcaattgt
      661 cgtactgtag ccgctatggc cgagggtgct tgcatttatt ttcaattcga cacctgttaa
      721 gaaaggatcg gaaaagttcg aaacgtaagt gtttgctgga agtccttcgc tggcatccac
      781 ccacgacatt ccatcatcgt cagataggaa cactccgttg gatgaggttc cagcataaat
      841 aattcttgga tctccatcga gagaaatctt ttcccgattc ttgataattg gaatttcaat
      901 agaagccaaa caattcactt gagaaggtaa ggagattatt ggactccaat tttcacctcc
      961 atcttctgtt tttactatat aatatcctgc tgcataataa ccacttccgg caaaaacctt
     1021 ttgtggattc gaaggatcaa cagctatagc ataagtttgg caacaattat tgggcaagcc
     1081 attgttaatt tctacccagt gatttccctg atcggtgctt ttcaacaaag atccattccc
     1141 tccataaacc acataacccg catagatcaa ttgttcatcg ctaaagggcg caatttcgag
     1201 tgcatggatc ttcatctgat ctgcaccaat ataaacttct tcccaacccg attgtccagc
     1261 atcgagggtt ctataaatag tagctgcata acctgcaact ccaaagtgat ttccacctat
     1321 gaaaagcaat ccgggttgat caggggtttg ttcgattgtc cagagttctg tttcgaataa
     1381 tggcccgata ttgggtccgg tattcgtcca agtaatacct ccatcggttg atttatagat
     1441 accttcctga gccaccgatg aagggcctct tgaaacggca tacacgtaac catgaatatc
     1501 gtaagttacg gccgagaatc ttgttgcagg gagacccgat accggattcc atgtatttcc
     1561 gccatcattc gacatataac aacctcccga attcaaggcc tcgaatgcga cgataatgtt
     1621 attacagtcg agtggatcga cggcaatgtc attgattgga agtgttgcaa tgccattact
     1681 gtaattttcc cagtttatac ccgcgttttg gcttctatat acagccaaac ttttgcatcc
     1741 aattaatatt tcttcaggat tattagggta aaatatcatg tcggtaatcg agaaattaga
     1801 agctccggta gaagaataat cccaattgag accaccatct gtcgaaaaat ataccccatc
     1861 accatcggtt cccgcgtaaa tcttcaaaga atctacaggg ctaacgagta cttttgcaac
     1921 taccttcaaa ggaaagttgg atgagatatt ctgccatgca aattcaccca gtttgcatgt
     1981 ataaaggcct aaatgctggc tcgaaaaaag ttgtccacct cctaaataaa ctgtatggcc
     2041 tgcaatttcg atatcattga aagggtatga attgggaaga ttatttgtaa ctgtttgcca
     2101 tgtttcgccc gagttattcg agtaaattac ttcacctcct ccgaatgcac cataagcagc
     2161 agccactaac tgagaaggat tgtcaggcca aactgcgata tcggcaacac ctagatcgac
     2221 attcagattc gaagtattca atatttgcca tgatgagcca ccatcggtag ttttgaggat
     2281 ttgtaaccag ttggtagccc ctaaggtgct ggccaggcca aggtatacaa tatcactatt
     2341 cgtaggatcg atggaaatag ccgaaatatc acgtaaatta attggtatag ttagttgtgt
     2401 ccaattgatg ccaccgtcgt tcgatttgaa aaagttccat gtattacccc cacttacata
     2461 tattattcca tcgggagcgg tagcaatgag attaacgcca gtcgtgtagg ctgccagact
     2521 ttcccataaa atccacgaag aagaagtatc atatttaatg aagggtaaac cagccgctgc
     2581 gtaaagcgtg gcaggcgttt caaacgaaga agccaaagag gtgacatcgc ctccccatgg
     2641 ccctatgctg ttccatggat aacccgcatc ctgattcgca ttaggaagtt ccaattgaga
     2701 ataattatta tgaaaaattt tcttcccata catcgtgtaa ggcaggttcc cattctcatc
     2761 aaacattatt tctttgtttg gcaactgaga caactcagat gaacagaaag aaattacatt
     2821 cgataaaccc ccctctccgg cgtaatttac taactctttc gattgtatcg aagaggaggt
     2881 agagcccgat gaaaaatcat gagcagcatt tcttggattc gaaacttgtg catttaaacc
     2941 ttgtgtttga aggattaata aaacaaatgc acatagccaa aacttgtaag ttttcatcat
     3001 tcattgattt ttaaggatta aacatttaat atcattactg accgacaaaa aaattgaata
     3061 aaatgaagga gggacaaaac cgaagtttca tcccccagtt gtaaatttgt gtagtaaac
//
LOCUS       DAOORU010000210         3104 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_37645_length_3104_cov_4.133158, whole genome shotgun sequence.
ACCESSION   DAOORU010000210 DAOORU010000000
VERSION     DAOORU010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37645_length_3104_cov_4.133158"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2998
                     /locus_tag="PLF20_09770"
     CDS             <1..2998
                     /locus_tag="PLF20_09770"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GH116 family glycosyl hydrolase"
                     /protein_id="HOX81324.1"
                     /translation="TKTLKDKGYGRMAGFHAFRSHPIFKDLNQGVYCLKPTRDITLEV
                     NGYFGDDSFPHRGKVIGVDWDYITLSEGNKVVWEYPSGKGKVLAVGGYILFSEDNINR
                     HHLEKFTSNCLYYLNNSDEYRPIPAFYWNNAQTSIKSFRLKDTLHLKEKTYSHFLAET
                     SSQPKVSKNANGQYYQVSGQRIVLMGKENGGIKEIWVHPFMAMYDYKVILQAPSPALP
                     VYLDSLIPEITIRPGCIKRHYQLQNNSLNEIIVAHPEKGIATIHYEFEGDEKTHLYIT
                     YQSNFRLMWPYSEKASRILRYSKGNKNRFHIITDFTQDYVCLTGSQRIPHQTKAGHFN
                     GFTFDEQARIIGHVTDEAIASCCYDYLLKSKDTLDFLIIAGSEGLQAALDNFSSTAKN
                     SARLSSEHEKAMKNLYEYHLQIETPDTLFNEAWKWYLAGINSFYIETPQLGRSLVAGF
                     NWTGTGWNGGHRIDGRPGYSWYFGRDSEWSALAILDYGDFEKAKDVLSMLSDYQDLNG
                     KIFHELTTSGFVHYDAADATPLYIVLACRYLRHTGDTAFIRSIWPNLLQAYKFCKSTD
                     TDYDHLIENTNVGHGWVEGGPLYGSHTSLYLASCWNEALIAMAEMASVMNEDSLAHEC
                     SKEAYIVNHLLNDSFYDTHEQSWRQGLMKDGTWHKLESVMPAVPALFGQMKEESLQTF
                     IEKTLGNEYITNYGSRILSNLSPYYHPHAYHAGSVWPLFTGWHAMAAYKANAFETGFR
                     LIMANMNIFRHWGYGYSEEVLNGDVYEPAGVCRHQCWSETMAIQPVAEGMLGFEPNAL
                     QNILRIAPALPPHWNSIHIHNLRFKESLIHFTMHTDDFKQSWQFNIHGNCSPQVIFAP
                     YFPVGSRINSIAINGQSAHFEEVINERYTQIVIYFKLDNSTRIEINYEPGISIIPEIN
                     LPEPGDKADGLRIIQSYIVDSVYVIEIEAPQNTQHTIKIKTSGERQYLIDGGYLKSCK
                     DRIIEIGVNFQDSEKPYVRKKIRLKI"
BASE COUNT          990 a          600 c          648 g          866 t
ORIGIN      
        1 aacaaagaca ttgaaagaca agggttatgg ccgcatggca ggttttcatg cctttcgttc
       61 gcatcctatc ttcaaggact tgaatcaagg agtttactgc ttaaaaccca ccagggacat
      121 tacgctcgag gtcaatggat atttcggtga tgattcattt cctcaccgag ggaaagtgat
      181 aggcgtcgat tgggattata tcactctatc agaaggaaat aaagtagtat gggaatatcc
      241 atcaggtaaa ggaaaagtat tggctgtggg aggatacata ttattttcgg aagacaacat
      301 caatcgccat catctcgaaa aatttacatc gaattgtctg tattatttga acaattcgga
      361 tgaatatcgt ccgattccgg ccttttattg gaataacgcc caaacttcga taaagtcatt
      421 tcgtcttaaa gataccttgc atctcaaaga aaaaacctat tctcattttc tggccgagac
      481 atcctcacaa cctaaagtaa gtaaaaacgc aaatggccaa tattatcagg tttctgggca
      541 aagaatcgta ttaatgggaa aagaaaatgg tgggataaaa gaaatttggg ttcatccttt
      601 tatggcaatg tacgattata aagtcatttt acaagcgcct tcacctgctc tgcctgttta
      661 tctggacagt ttaatacccg agatcaccat tcgacctggt tgtataaaac gacactatca
      721 actgcagaat aactccttga atgaaatcat cgtagcccat cccgaaaagg gcattgcaac
      781 aattcattat gaattcgaag gggatgaaaa aactcatctg tatataactt atcagagcaa
      841 ttttcgtttg atgtggccct acagcgaaaa agcttcgagg attttgcgtt acagcaaggg
      901 aaacaaaaac agatttcaca taattacaga ttttacacag gactatgtct gtttgacagg
      961 ttcacaaaga attcctcatc aaacgaaagc cggtcatttt aatggcttca cctttgatga
     1021 acaggcaaga attattggcc atgttactga tgaggcgata gcatcgtgtt gctatgacta
     1081 tttactcaaa agcaaagata ccctcgactt tctaattatt gccggaagcg aaggattaca
     1141 ggcagccctc gacaattttt cttcaacagc aaaaaattct gctcggttat cgagcgagca
     1201 cgaaaaagcg atgaaaaatc tttacgaata tcatcttcag attgaaacac cagatacact
     1261 ttttaatgaa gcatggaaat ggtatttggc aggaataaac agtttctaca tagaaacgcc
     1321 acagttggga aggtcactgg tagcaggctt caattggaca gggaccggct ggaatggagg
     1381 ccatcgcata gatggaagac ctggatatag ttggtacttt ggaagagata gcgaatggtc
     1441 ggctttagcc attttagact atggcgattt tgaaaaggca aaggatgtct taagcatgtt
     1501 aagcgattat caagacctca acggtaaaat ctttcatgaa ctaactacca gcggttttgt
     1561 ccattacgat gctgctgatg ccactccttt atatatagtt ctggcttgcc gatatcttcg
     1621 ccatacggga gatacggctt ttatccgttc aatatggcct aacttactcc aagcctataa
     1681 attttgcaaa tctaccgata ctgattacga tcatttgatc gagaacacca atgttggaca
     1741 tggttgggta gaaggcggcc cattatatgg ttctcatact tcactttatc tggcttcgtg
     1801 ctggaatgaa gctctaattg ctatggctga aatggcatca gtgatgaacg aggatagcct
     1861 cgcccacgaa tgcagcaagg aagcttatat tgtgaaccat ctcttgaacg atagctttta
     1921 cgatacgcat gagcaatcgt ggcgtcaggg tttgatgaaa gatggcactt ggcataaatt
     1981 agaatcggtc atgccagcag ttccagcatt atttggtcaa atgaaggagg agtctctcca
     2041 aacatttatt gagaaaacat tgggaaatga atatattact aattatggca gcagaatttt
     2101 gagcaatctt agtccatact atcatcccca tgcttatcac gccggctcgg tatggccgct
     2161 ctttaccgga tggcatgcta tggcagctta caaagcgaat gctttcgaaa ctggatttag
     2221 gcttatcatg gcgaacatga atatttttcg tcattggggc tacggatatt cagaggaggt
     2281 attgaacgga gatgtatatg aacctgcagg tgtatgtcgg catcaatgct ggtcggagac
     2341 aatggccatt cagcctgtgg cagaaggaat gctgggcttc gaaccaaatg ccttgcaaaa
     2401 tatccttcgg attgcaccag cattgcctcc ccactggaat tcaatccata tccataatct
     2461 ccgattcaaa gaatcgctca ttcattttac tatgcatacc gatgacttca aacaaagctg
     2521 gcaattcaat atccatggaa attgttcgcc ccaagttatt tttgctcctt atttcccagt
     2581 aggaagccgg ataaattcga tagcaataaa tggacaatcg gctcatttcg aagaggttat
     2641 aaatgaaaga tatactcaaa ttgtaattta tttcaagctg gataattcta ctcggataga
     2701 aataaattat gaaccaggga tttctattat cccagaaatt aacttacccg agccaggaga
     2761 caaagcagat ggactgagaa tcatacaatc ttatatagtt gattcggttt atgttataga
     2821 aattgaagca ccacaaaaca ctcaacatac tattaaaata aagacatcag gcgaaaggca
     2881 gtatttgatt gacggtgggt atttaaagag ttgcaaagat agaattatag aaatcggagt
     2941 caactttcag gattccgaaa aaccttatgt aagaaaaaaa atcaggctta agatataata
     3001 gagacaaaaa agccgtccag tcttattgat tggacggcta agctcaataa tcaagctatc
     3061 ggaattactt gatagaatat gtcatagtag ccaggtcgac ctta
//
LOCUS       DAOORU010000211         3101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_37703_length_3101_cov_4.049573, whole genome shotgun sequence.
ACCESSION   DAOORU010000211 DAOORU010000000
VERSION     DAOORU010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37703_length_3101_cov_4.049573"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLF20_09775"
     CDS             complement(<1..322)
                     /locus_tag="PLF20_09775"
                     /inference="COORDINATES: protein motif:HMM:NF012308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HOX81325.1"
                     /translation="MKNFLFLSIPMIVLPIILSISSCSKDDETPKQPKPIVLPEKSAK
                     VISCTNDFGIELFSLVAQGNDDNLMLSPLSASIAFTMALNGSKGNTWQQISDLLGFHD
                     LTQEE"
     gene            683..1957
                     /locus_tag="PLF20_09780"
     CDS             683..1957
                     /locus_tag="PLF20_09780"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOX81326.1"
                     /translation="MEVFKFGGASLKDAQSIKNIVEILNSYHDLEQLVVVVSAMGKTT
                     NAFEKFLSLVYSGSANFQEAITPIKSFHWEVCRQLFAPEHYFWKELEAIFDEIREQSA
                     ELSALPFDMAYDKVVGYGEILASRIFSVYLNTQGIDHQWLDARSVIRTDSCYRAANVD
                     IELSYDRIRVWFQQNTDSRYRIITQGFIGQDMQGNMVTLGREGSDYSAALFATALSAN
                     SVVLWKDVPGVMNADPDIYAQAVPFKFVSYREAVELTYYGAKIIHPKTIKPLENAGIP
                     LYVRSFLHPSEQGTLIGPGNGTIPQIPSIIHKANQILITISHKDLSFITEKTLLTVLS
                     VFDNSSLKINLMQNSALTFSVCLDDVKGKIDQILTRLMESYEVKYNRQLTLLTFRHYA
                     QQPRFIDQWIEGRSVLVEQRSRNTAQYVIAEK"
     gene            1979..>3101
                     /locus_tag="PLF20_09785"
     CDS             1979..>3101
                     /locus_tag="PLF20_09785"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HOX81327.1"
                     /translation="MKKYFFFSFLTFLIFYFPFVLQSQHRQQISLDKSWKFHLGSASS
                     AEKDFGYGLVNIYSKNGKSEGTAIYPGFDDSNWRRVNLPHDWAIELPFINHPSFDVMS
                     HGYKPVGGFFPETSIGWYRRSFDISRSDSGYRFSLTFDGVYRDAKFWLNGYYLGNNQS
                     GYSGVEFDITDFVWFDKPNTIVVRVDATQYEGWFYEGAGIYRHVWLNRYPEIHIPSDG
                     VFAYSIPKGKSCIIHFETTISNSSLNDKMVSMETKVKSRDGRLIAQNMSKSVRTASSG
                     QTRVNQQIKIDNPHLWNLDDPYLYRIETSILSEGKILDKVSMPFGIRFITWDADKGFF
                     LNNKPVKLLGVNMHQGHAGLGVALPDGMQYYRINRLKQMG"
BASE COUNT          921 a          612 c          604 g          964 t
ORIGIN      
        1 cttcttcctg cgtcaaatca tgaaaaccca acaggtcgct gatttgttgc caggtgtttc
       61 ctttagagcc attcagtgcc atagtaaagg ctatgcttgc actcaaggga gataacatca
      121 aattgtcatc attgccctgt gctaccaaac tgaatagttc tatgccgaaa tcgttggtgc
      181 aagaaatgac ttttgccgat ttttcaggta aaacgattgg tttgggttgt tttggagttt
      241 cgtcatcctt actgcatgaa cttatactaa gaattattgg caaaacaatc atgggaatgg
      301 agaggaataa gaagtttttc atacttgatg atgtttgaaa tgtataagaa aaaatagcaa
      361 taatcaaacc ataaatattt gaaataggat taaatgagtt attttaattc agaaccatta
      421 tttcgggtaa atattggaaa atttggattg cttagccatc gaatcgccca aaagggcttt
      481 tctaaatcac ccttttgctt ttgacgcttc aatactttgc cttaataaac aaattatgtc
      541 ctactacaaa caatagtttt cgcgacgcta agtattctac aaacttcgta agaatgatat
      601 aggctaattt ttttgatgaa ttgccttatc atttctcaaa tctttatctt attacttatt
      661 tttgctattc tatttttata aaatggaagt cttcaaattt ggtggagctt cgctgaagga
      721 tgctcagtca ataaaaaata tagtagaaat acttaactca taccatgatt tagagcaatt
      781 ggtggttgta gtttcggcca tggggaaaac cacaaatgcc tttgagaaat tcctctcgtt
      841 agtctatagt ggatcggcta attttcagga agctatcact cccattaagt cttttcattg
      901 ggaggtttgc cgtcaattat ttgccccaga acattatttc tggaaagaat tggaggctat
      961 tttcgatgaa atacgggagc aatctgctga attatctgca ttgccttttg atatggccta
     1021 tgacaaagtt gtgggttatg gagaaattct tgcttctcgg attttcagtg tttacctgaa
     1081 tactcaaggc atagatcatc aatggctgga tgcacgttca gtaattcgaa ccgatagctg
     1141 ttatcgcgct gccaatgtag atatcgaact ttcgtacgat cgaatcagag tatggtttca
     1201 gcaaaatacc gattcacgat atagaataat tactcaaggt tttataggac aggatatgca
     1261 gggcaacatg gttacgcttg ggcgcgaagg ttccgattat tctgctgcct tgtttgccac
     1321 ggcactttca gccaatagtg ttgtgctttg gaaagatgtc ccaggcgtga tgaatgcaga
     1381 ccccgatatt tatgctcagg ccgtgccttt taaattcgta tcctaccgcg aagccgtcga
     1441 actcacttat tatggagcta agataataca tcccaaaacc atcaaacctt tggaaaacgc
     1501 aggaatccca ttgtatgtcc gatcttttct ccatccgtca gaacaaggca cactcattgg
     1561 accgggaaac ggaactattc cacaaatccc ctctattatc cataaagcca accaaatttt
     1621 aattactatt tctcataaag acctttcctt catcacagaa aaaacccttt tgacagtttt
     1681 atctgttttt gataattcct ccttgaagat caatttgatg caaaattctg ctctcacttt
     1741 ttcggtatgt ttggatgatg taaaggggaa aatcgaccaa atccttactc gactcatgga
     1801 atcttacgag gtaaaatata atcgccaact taccttgttg accttccgtc attatgctca
     1861 gcaacctcga tttatcgacc aatggataga aggaaggtcc gttttggtgg aacaacgcag
     1921 ccgcaacact gcccaatatg ttattgctga aaaatgatgt cctaataata attaagccat
     1981 gaaaaaatat tttttctttt cgtttctgac atttctaatt ttctattttc catttgtatt
     2041 acaaagccag catagacagc aaatttctct ggataaaagc tggaaatttc atttggggag
     2101 cgcttcatca gccgaaaaag atttcggtta tggattggta aatatctatt caaaaaatgg
     2161 caaatcggaa ggtacggcca tttatcccgg ttttgatgac agtaactggc gtagggtaaa
     2221 tttgcctcac gactgggcta tcgaacttcc tttcataaat catccctctt tcgatgtaat
     2281 gagtcatgga tataaacctg ttggcggatt tttccccgaa acctctatag gttggtacag
     2341 gcgaagtttt gatatttcac gttccgattc aggctatcgg ttctctttaa ccttcgacgg
     2401 ggtctatcga gatgcaaagt tctggctgaa tggatattat ctgggtaaca accaaagcgg
     2461 atattcaggt gtagaattcg acattactga cttcgtctgg ttcgacaaac ctaatactat
     2521 agtcgttcga gtggatgcca cccaatacga aggttggttt tacgaaggag caggcattta
     2581 tcggcatgtc tggctgaatc gctatcccga aattcatatc ccttctgatg gtgtctttgc
     2641 ctacagcatt cccaaaggaa agagttgcat cattcatttc gaaactacca tctcaaactc
     2701 atctttaaat gataaaatgg tgagtatgga aacaaaagtc aaaagtcgtg atggcagact
     2761 aatcgcccaa aacatgtcta agtcggttcg cacggcttct tcaggacaaa ccagggtaaa
     2821 ccaacagatt aaaatagata atccccactt gtggaacctt gacgatcctt atctttaccg
     2881 tatcgaaacc agtattcttt cggaaggtaa aatcttggat aaagtaagca tgccatttgg
     2941 aatacgtttt atcacctggg atgccgacaa aggttttttc ctaaataata aacccgtaaa
     3001 actattaggg gtaaacatgc atcaaggaca tgcaggcttg ggtgtagcct taccggatgg
     3061 aatgcaatat tatcgcatca accgacttaa acaaatggga g
//
LOCUS       DAOORU010000212         3084 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_37982_length_3084_cov_4.399472, whole genome shotgun sequence.
ACCESSION   DAOORU010000212 DAOORU010000000
VERSION     DAOORU010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37982_length_3084_cov_4.399472"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..>3084
                     /gene="gcvP"
                     /locus_tag="PLF20_09790"
     CDS             308..>3084
                     /gene="gcvP"
                     /locus_tag="PLF20_09790"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOX81328.1"
                     /translation="MMTNNFAERHIGPTPLDVEKMLQYIGVSSLDELIAQTIPSGIRL
                     KGPLHIEQGMNEYEVFHHLKTLGQKNKLYRSFIGMGYYNTILPGVIQRNILENPGWYT
                     SYTPYQAEISQGRLEALLIFQTMVADLTGLPISNSSLLDEATAAAEAMIMAYNCRSRS
                     KMKTNANKFFVSENLFPQSIALLQTRSVPLGIELVIGNHDKVQLDDGFFGGIVQYPDG
                     FGQINNYRAFAEKIHSVEALVVVAADILSLVLLTPPGEWGADIVIGSTQRFGIPMSYG
                     GPHAAFLASKEEFKRSIPGRIIGISIDTQGRPSLRMALQTREQHIKREKATSNICTAQ
                     ALLAIMAGMYTVYHGPEGLHKIARHINILTGVLANEASKYGYNQLNSAFFDTVRLRLP
                     EQVNIKTIKNLALKRQFNLRYIDDQTLGISLDETTSLKEVNQILEILSLAANRTFQEF
                     KCNPVECENIVTFPQEFIRRSAYLPHPVFNSYHSETAMMRYLKKLENRDLALNRTMIP
                     LGSCTMKLTAGVEMFPLSWSEFNGLHPFVPADQAEGYHLMINELEALLKEITGFSAFT
                     FQPNSGANGEYTGLMIIRKYHESRGEGHRNVCLIPSSAHGTNPASAHFAGMDIVVVKC
                     DENGNVDIACLREKAVQYKDHLAALMITYPSTHGVFEEEILEIVNIIHQNGGQVYMDG
                     ANMNAQVGLTSPGRIGADVCHLNLHKTFAIPHGGGGPGVGPVGVASHLIPFLPQHPIV
                     KTGGQQGISPVAAAPYGSALVLTISYAYMKLLGRDGLTNASKYAILNANYLKARLENH
                     YKILYTGKNGRVAHEMILDCNKFQLSADVSVIDIAKRLIDYGFHAPTVAFPVHGTLMV
                     EPTESEPLEELDRLVDALIAIRKEIAASENHQADRQNNLLKHAPHTAAMVVSSQWNYP
                     YTREKAAFPLPG"
BASE COUNT          921 a          686 c          633 g          844 t
ORIGIN      
        1 aactcgcttc cccaaaggcg gtataacttg ggtaggattt ataggagaaa aatagcatct
       61 ttcaccagat tttcgattgg tttatcactt tatattcata aaaacagagc caacccatta
      121 agaatacaac aaatcaaaaa taaaggcatt aaaaaaacga aacgtatggt aaatagctga
      181 agttattcca tgaatggatt atggcataga caagaaaatt tgtgttgcag cactaggatg
      241 tctaattttg ccaaagcata aatttttctg gatttgcttt caaacaactg taaaacaata
      301 aataagcatg atgaccaata attttgcaga gcgccatata gggcccaccc ctctggatgt
      361 tgaaaaaatg cttcaatata ttggggtttc ctcattggat gaattgatag ctcagaccat
      421 tccttcggga attcgactaa aaggcccact tcatatcgaa cagggaatga acgaatatga
      481 agtatttcat cacttaaaaa ctttaggaca aaaaaataaa ctctatagaa gttttattgg
      541 catgggatat tacaatacta tcctgcccgg tgtaattcag cgtaatattc tcgaaaatcc
      601 gggatggtac acttcttata caccttatca agccgagatt tcgcaagggc gtttagaagc
      661 attgctcatt tttcagacca tggtagccga tttaacgggt ttgcccatca gcaacagttc
      721 attgctcgat gaagccaccg ctgctgctga ggctatgatt atggcataca actgccgctc
      781 acgttcaaaa atgaagacca atgcaaacaa gttttttgtt tcggaaaacc ttttccctca
      841 aagtattgct cttctacaaa caaggtcagt tccattggga atagaactgg tcattggcaa
      901 tcacgataag gtccaactgg atgatggttt ttttggtggc atagttcaat atcccgatgg
      961 atttggacaa attaacaatt acagagcttt tgcagagaag atacattccg tggaagcgct
     1021 ggtggttgtt gcagcagata ttctttcatt ggttctgcta acgcccccag gagaatgggg
     1081 agccgatatt gtgattggtt cgacccagcg ttttggaatt cccatgagtt atggtgggcc
     1141 acatgctgca tttttagcat cgaaagaaga gttcaaacgc tcgataccgg gtcgaattat
     1201 cggcatatca atagatacac aagggcgtcc tagcttacgc atggctttgc aaactcgcga
     1261 gcaacacatc aaaagagaaa aggccacttc caacatctgc accgcgcaag ctcttcttgc
     1321 catcatggca ggtatgtaca ccgtatatca tgggccagaa ggcctgcata aaattgcacg
     1381 ccatatcaat atccttactg gcgttttggc caatgaagct tccaaatatg gatacaacca
     1441 actcaattcg gccttcttcg atactgttcg actccgcctt cctgaacaag taaacatcaa
     1501 aaccattaaa aaccttgcgt tgaagcgcca attcaatttg cgctatatcg atgatcaaac
     1561 acttggcatt agcctcgatg aaaccacatc cttgaaagaa gtcaatcaga tactcgaaat
     1621 tctttcctta gctgcaaatc gaacttttca ggaatttaaa tgcaatccgg tcgaatgtga
     1681 aaatatagtt actttccctc aggaatttat tcgtcgaagt gcatacctcc ctcatcctgt
     1741 tttcaattct taccattccg aaactgccat gatgagatat ttaaaaaaac tcgagaatcg
     1801 ggatttagct ctcaatcgaa cgatgattcc cctaggaagc tgcaccatga aactcactgc
     1861 tggtgttgaa atgttccctt tgagctggtc cgaattcaat ggtttgcatc cttttgtacc
     1921 cgccgaccaa gccgaaggtt atcatttgat gatcaacgaa ttggaagctc tcctaaagga
     1981 aatcacggga ttttcggctt ttacttttca acccaattca ggagctaacg gagaatatac
     2041 cggattgatg atcattcgta aatatcatga aagcagagga gaaggccatc gtaatgtgtg
     2101 tctcattccc tcatcggctc acggaaccaa tcctgcctcg gctcactttg ccggaatgga
     2161 tattgtggtt gttaaatgcg acgaaaatgg aaacgtcgat atcgcttgtc tgcgtgaaaa
     2221 agccgttcaa tataaagatc atctagcagc tttgatgatc acttaccctt ccacgcatgg
     2281 agttttcgaa gaagaaatcc tcgaaatcgt gaatatcatt catcaaaacg gaggtcaggt
     2341 ttatatggat ggagccaata tgaatgcaca ggtaggattg acgagtccag gacgcatagg
     2401 agcagatgtt tgccatttga accttcacaa gacttttgct attcctcatg gaggaggagg
     2461 gcctggagtt ggaccagtag gagtcgccag ccatctgatt cctttcttgc ctcagcatcc
     2521 tatcgtaaaa actggtggcc agcaagggat atcgcccgta gcagctgcac cttatggtag
     2581 cgctcttgtt cttaccatat cttatgctta catgaaactt ttaggaagag acggacttac
     2641 gaatgccagt aaatatgcca ttctcaatgc aaactattta aaggctcgcc tcgaaaatca
     2701 ttataaaatc ctctatacgg gcaagaatgg tcgtgtagcc cacgaaatga ttctcgattg
     2761 taataaattt cagctttcag ccgatgtatc ggtcatcgat attgccaagc gtttgatcga
     2821 ttatggcttt cacgccccaa ccgttgcttt cccagtacat ggtacactaa tggtagaacc
     2881 caccgagagc gaacctctcg aagaactcga ccgactggtg gatgcattaa ttgctatcag
     2941 aaaagaaatt gccgcttctg aaaaccatca ggcagaccgc cagaacaacc tcttgaaaca
     3001 tgctccccat accgcggcaa tggttgtaag ttcacaatgg aattatcctt atacccgcga
     3061 aaaggcggcc ttccccttgc ccgg
//
LOCUS       DAOORU010000213         3025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_39064_length_3025_cov_5.046801, whole genome shotgun sequence.
ACCESSION   DAOORU010000213 DAOORU010000000
VERSION     DAOORU010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39064_length_3025_cov_5.046801"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1721
                     /locus_tag="PLF20_09795"
     CDS             <1..1721
                     /locus_tag="PLF20_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOX81329.1"
                     /translation="ALSLTPMLSSRILKSNFYYNYHPNKSLAGVSRFWGYFDEWYAKI
                     LSYALRHKKLILSLAFVIFIGSLFLFKFIGTEFIPEQDNGRIGINIEIPQGVRIDESK
                     KLARDFEEVMKQKYPEIELVSTSIGIDEEGSLASAVQRAGSYIINITLRLSSAEERSR
                     SIYDIADSLRNDLTKYPIIASSYVSVGGSRSQMGISMVGGGGNNVVVNIFGYDINKTN
                     YIADQLSSFMKTQPGFRDVTISRDKDRLELQVIPDRTKLAQLGMNTAMLSSAIRNRVL
                     GFKATKYREEGNEYDVIVKYNDENLVSVDDIRNITFRGPQGNLIRVNDVAEVKEVYTT
                     PNIEHENKIRVVRVEASLKGTDLGKATAQINSKLKTLDVPPQIFVEISGAAKDMADTF
                     RDLGLLSILILLLTYIVMASQFESLREPFIIMFSVPFAITGVLISLFITHTSINIISG
                     IGIVMLIGIAVKNSIVLVDFINLLVGRGYSIRDAIIAGGKSRLRPILMTSFTTLLGML
                     PLAISHGEGSEIWRPLGISIIGGLFFSMLISLIIVPVIYSLFAHAKKRREENNKIPQE
                     LHLPSE"
     gene            1789..2082
                     /locus_tag="PLF20_09800"
     CDS             1789..2082
                     /locus_tag="PLF20_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81330.1"
                     /translation="MKAVFIIFNQAITNKVEKALELAQIKGFSKWNEMVGAGSNEGEP
                     HLGTHVWPSLNSGIFTVLEDEKVKTLLNLIEQINNIAEAQGIHAFVWNIETMV"
     gene            2177..>3025
                     /locus_tag="PLF20_09805"
     CDS             2177..>3025
                     /locus_tag="PLF20_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81331.1"
                     /translation="MSRRQRKRKTAQNLEIMGMHSSGKGWTLFQGRKVYFPYTLPGEI
                     ISGEIIYGGSEFLTGNLISIEKPHPQRRTHICKHSGLCGGCSWQHIQYSFQLLMKCQI
                     LEEALNHKGIPFKEIKPVVPSENEFFYRNRLEYSFSAQRWFYDNEGKINNTKERQAVG
                     FNVSQLNDRIIDITECYLQPEPSVFIALQAKKFAQSLNLSFFNFKDGSGMIRSIEILQ
                     DNTPKVILMVGFAIQPNEEAFRFLDHLYNDLSAVKSVFWKIYSNPFKSFGEKEVHLFK
                     GEYRFLT"
BASE COUNT          938 a          588 c          608 g          891 t
ORIGIN      
        1 cagctttgtc gctcactccc atgctttctt cgcgcatttt aaaaagcaat ttttattata
       61 attatcatcc taataaatct ttagccggtg ttagccgctt ctggggttac ttcgacgaat
      121 ggtatgcgaa gattctaagc tatgcccttc ggcataagaa gcttatcctt tcattggcct
      181 tcgtcatatt tatcggaagc ctgttcttgt ttaagttcat tggaacagaa ttcattcccg
      241 aacaagataa tggaagaata ggaattaata ttgaaattcc tcagggagta agaattgacg
      301 aatccaagaa attggctcgc gatttcgaag aggttatgaa acaaaaatat cccgaaatcg
      361 aattagtttc gaccagtatt ggcatagatg aggagggttc gcttgcaagt gccgtacaac
      421 gtgcaggttc ttacattatc aatataacct taagattgag cagtgcagag gaacgttcgc
      481 gcagcattta cgatattgcc gatagcttga ggaatgatct gacaaaatat cctatcattg
      541 cttcttctta tgtgagtgtg ggtggttcgc gaagtcaaat gggtatatct atggtgggtg
      601 gtggaggaaa taatgtagtc gtcaatattt ttggttacga catcaacaaa actaattata
      661 ttgccgatca gctctcctct ttcatgaaaa ctcaacccgg tttcagagat gttaccattt
      721 ctcgtgataa agaccgtctt gaacttcagg taatacctga taggaccaaa ctggctcaat
      781 taggcatgaa tacggctatg ctgtcgagtg ccatcagaaa tcgagtgttg ggatttaagg
      841 ccacaaaata cagggaagaa ggcaacgaat acgatgtaat cgtaaaatat aacgatgaaa
      901 atttggtgtc ggtggatgac atacgaaaca ttacgtttcg tggtcctcag ggcaatttga
      961 taagggtaaa cgatgtggcc gaagtcaagg aggtttatac aactccaaac atcgaacacg
     1021 aaaacaaaat acgcgttgta agggtggagg cttcactcaa aggaactgac cttggaaagg
     1081 caacggccca gataaattcg aagctaaaaa ctctcgatgt tcccccacaa atatttgtcg
     1141 aaattagtgg agcagcaaaa gacatggccg atactttcag agatttgggg ctgttgtcca
     1201 ttcttatcct tctgctcact tatatcgtaa tggcttcgca attcgagtca ttgcgagaac
     1261 ctttcatcat catgttttct gtaccttttg ccatcactgg agttctaata tcattattca
     1321 tcacacatac cagcattaat atcatttcgg gcattggaat agttatgctg attggaattg
     1381 cagtaaaaaa ctccattgtt cttgtagatt tcatcaacct gctcgtgggc aggggctatt
     1441 ccatcaggga tgccattatt gccggaggta aatcgaggtt aagacctatt ctcatgacct
     1501 cttttacaac cctccttggt atgctccctc tggctatttc tcatggggaa ggttcagaaa
     1561 tctggagacc tctgggtata tccatcattg gtggtttgtt tttctcgatg ctcatctcgc
     1621 tgatcatcgt acccgtaatt tacagtttgt ttgctcacgc caaaaagaga agagaagaaa
     1681 acaataaaat tcctcaggaa ttacacctcc cttccgaata atatccgtta ggtgtcagcg
     1741 ctatttctcg aagtgctgac acctcatttt catctaaaaa acaaaaaaat gaaagcagta
     1801 tttataattt ttaatcaggc gattaccaat aaagttgaaa aggccctaga attagcacaa
     1861 ataaaaggct tcagcaaatg gaacgaaatg gtcggtgcgg gctcaaatga gggggagcct
     1921 catcttggta cccatgtctg gccatcttta aattcaggta tctttactgt attggaagac
     1981 gaaaaagtga aaaccttgct caatttaatc gaacaaatca acaatatagc agaagcacag
     2041 ggtatacatg ccttcgtctg gaatatcgaa actatggttt aatcaaccct aaatctcctt
     2101 aatcctatcc ccctgaataa ttataaaacc accttaagtt ttcccgactg aggtggtttt
     2161 tatatttttg aaacgcatga gccgtcgaca aagaaaacgg aaaaccgccc agaatttgga
     2221 gataatgggc atgcattcat caggaaaagg atggacccta tttcaaggac gaaaggttta
     2281 tttcccttat accctaccgg gtgaaatcat ttcaggagaa ataatctatg gaggctcgga
     2341 atttttaacc ggaaatttga taagtataga aaaaccgcat ccacaaaggc gaacacatat
     2401 ttgtaaacat tctggcctgt gtggcggatg ttcatggcag catattcaat attcatttca
     2461 gctactaatg aagtgtcaaa tccttgaaga agctttaaac cataagggaa tcccctttaa
     2521 agaaattaaa ccagtcgttc catccgaaaa tgaattcttt tacagaaatc gcttagaata
     2581 ctcattttca gcccaaagat ggttttatga taacgaaggg aaaataaata atacaaaaga
     2641 acgccaagca gtcggtttca atgtttctca attaaatgac aggattatcg atattactga
     2701 atgctatcta caaccagaac cttcagtctt cattgcccta caagccaaaa aatttgctca
     2761 gagcctgaat ttgtcttttt tcaatttcaa ggatggcagt ggtatgattc gtagtattga
     2821 aatcctgcaa gacaatacgc ctaaggtgat attaatggtt ggttttgcca ttcagccaaa
     2881 tgaagaagca tttaggtttc ttgatcatct ttacaatgat ttgtcagccg taaaatcagt
     2941 tttttggaag atttattcca atccctttaa gtcctttggt gaaaaagagg ttcatttatt
     3001 caaaggtgaa tatcgatttc ttaca
//
LOCUS       DAOORU010000214         2807 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_43289_length_2807_cov_3.183866, whole genome shotgun sequence.
ACCESSION   DAOORU010000214 DAOORU010000000
VERSION     DAOORU010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43289_length_2807_cov_3.183866"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..509)
                     /locus_tag="PLF20_09810"
     CDS             complement(<1..509)
                     /locus_tag="PLF20_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81332.1"
                     /translation="MKNRLITVIGVLVVVLGACETDIDVNADWKDITTVYSILDPAEK
                     TQYVRINKAFLGEGNALEYAQIADSINYDTSYIEARIDEYLKGELVQSMKLKPLWIPR
                     KNDPNSPFYNPNFPYVLVYMAQPKNFYKMSGNDTIWLNPDSDYHLEIFNRHSGKTVTA
                     STIIVKDFN"
     gene            642..>2807
                     /gene="selD"
                     /locus_tag="PLF20_09815"
     CDS             642..>2807
                     /gene="selD"
                     /locus_tag="PLF20_09815"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOX81333.1"
                     /translation="MNLIYLDYNATTPLATEVIEAMKPFMEVYFGNPSSAYVLGQKTR
                     LAVEEARRQVSTLLGCQPDEVIFTSGGTESNNMAIKGAALANRSKGNHIITSCIEHPS
                     VLEVCHWLESHGFRITYLPVDEFGRVDLDAFRAAISEETIIVSIMHSNNETGSLQPVQ
                     EIGMIAKQHGILFHVDAAQSIGKIPVKVDDFCVDLLSVAGHKFYAPKGIGALYIRRGV
                     KIEKIIHGASQEFGWRPGTENVIHIAGLGMACKLVNERLENDHVHLKSLRDRLQAGLF
                     QRIDGVKLNGHPELRLPNTLNVSIAGIEANALISELSDIAVSTGAACHSQKQEASHVL
                     EAMHLSPEFAMGTLRFSLGRYTTEDEIDRAVEEIILTVKRLRRENPPLWVCRETDIKL
                     THFTQGLGCACKLQPAVLEEVIKKIPLLISSPNLLVGYETSDDACVYKTDENLALVSA
                     LDFFTPIVDDPYQFGAIATANSLSDIYAMGAKPLFALNIVAFPSHRLPLAVLENILKG
                     ATDKAAEAGIPIMGGHTIDDAELKFGLAVTGTIHPNKIMRNCGAKPGDRIILTKPIGS
                     GILSTALKQGLLQKSTEEIFLKNLLTLNKKASEIMLSFPVTACTDVTGFGLLGHLMEM
                     CKPGHLGAILYRRAIPLLPEVEIFAMAGIVPGGSKGNLEYVKNDVIFTEDIPLPFQYI
                     YSDAQTNGGLLIAVMSEEAENLLESLHEAGIHQASLIGEF"
BASE COUNT          818 a          533 c          635 g          821 t
ORIGIN      
        1 atgttgaaat ccttcactat aatggtggaa gcagtaacag tttttcccga atggcgattg
       61 aagatttcca gatgatagtc gctatcggga tttagccaga tggtatcgtt tccagacatc
      121 ttatagaaat tttttggttg agccatataa accagaacat aagggaaatt gggattgtaa
      181 aaggggctat tggggtcatt ttttcgtgga atccataagg gcttcagttt cattgactga
      241 acgagctcac ctttaagata ttcatctata cgtgcctcta tgtaagaagt atcataattg
      301 atagaatccg ctatctgagc atattccaat gcattgcctt cgcccagaaa ggccttgttg
      361 atgcgaacat actgcgtttt ttcagccggg tcgagaatac tataaacagt agttatatct
      421 ttccaatcgg catttacatc tatgtcggtt tcacatgcac ccaggacgac tactaaaaca
      481 cctatcaccg taatcaatct atttttcata atatttcctc attattcgaa acttaatgtc
      541 ttagattcag atttcctgat ttggcttaaa atgcaaaggt aaaattattt aactatcgat
      601 agataatatt ttcgagactg tctaattttg ccgaaaaaac gatgaatctc atttatctgg
      661 attataatgc cactacacct ttggccacag aagtaatcga ggccatgaaa ccgtttatgg
      721 aggtttattt tggaaaccct tccagtgctt atgttctggg gcaaaaaacc aggttggccg
      781 tcgaagaagc gcgaagacaa gtttcgactt tattgggatg ccaacccgat gaagtcattt
      841 ttaccagcgg tggaaccgag tcgaacaaca tggctatcaa aggtgcagcc ttagccaatc
      901 gttcgaaagg caatcatatc attacttcct gtatcgagca cccatcggtg cttgaagtct
      961 gccattggct cgaaagtcat ggattcagga ttacctattt acccgtagat gagtttggcc
     1021 gggtggattt agatgcattc agggctgcta taagcgaaga aaccattatc gtgagcatta
     1081 tgcattccaa caatgagaca ggtagtctgc aacccgtgca ggaaatcgga atgatagcaa
     1141 agcagcacgg gattttattc catgtagatg ccgctcaaag catagggaaa atacctgtta
     1201 aggtggatga tttttgcgtt gatttattgt cggtagccgg gcataagttt tatgcaccaa
     1261 agggaatagg ggctttatac attcgaagag gagtaaagat agaaaaaatc atacatggtg
     1321 catctcaaga gtttggatgg aggccaggga cggaaaatgt gatacatatt gctggattgg
     1381 ggatggcctg taaactggtt aacgagcgat tggaaaatga tcatgttcat ctgaaatcat
     1441 tgagagatcg tttgcaagct ggattatttc agagaatcga tggcgtcaaa ctgaatggac
     1501 atcccgaact caggcttccc aatactttga atgttagtat tgcaggtata gaagcgaatg
     1561 ctttgatttc ggaactgagc gatatagccg tttcgacagg tgctgcatgt cattctcaga
     1621 aacaggaggc cagccatgtt ttagaggcga tgcatctttc tcccgaattt gctatgggta
     1681 ctttacgatt ttcgttagga agatatacca cagaggacga gattgaccga gccgtcgaag
     1741 aaattatttt gactgtcaaa agacttcgta gagaaaatcc tcctttatgg gtttgccgag
     1801 aaactgacat aaagttgacg cattttacac agggtttggg ttgcgcttgt aagctgcaac
     1861 ctgcggttct ggaagaagtg ataaaaaaga ttcctctgct catttcttcg cccaatctgc
     1921 tggtgggtta cgaaacttcg gacgatgcct gcgtatataa aactgatgag aatctggctt
     1981 tggtaagcgc tctcgatttt tttactccca ttgttgacga tccttatcag tttggagcca
     2041 ttgctacggc caattctttg agcgatattt atgccatggg agccaaaccg ctttttgcat
     2101 tgaatatagt tgcttttcca tctcatcgtt tacctctcgc agtgctcgag aatatcctca
     2161 aaggagcaac cgataaggcg gccgaagcag gcatacctat tatggggggg catacaatag
     2221 atgatgccga actcaaattt ggactggcag taacaggaac gattcatccc aataagatta
     2281 tgagaaattg tggagctaaa cctggtgatc gaattatctt gaccaaacca ataggttcag
     2341 gaatactgtc aaccgcttta aaacaaggat tattacaaaa atctaccgaa gagattttcc
     2401 ttaaaaattt acttacattg aataagaaag cctccgaaat tatgctttcc ttccctgtaa
     2461 cagcatgtac tgatgtaact ggatttggcc tgctgggaca cctgatggaa atgtgcaaac
     2521 ccggtcatct gggtgctata ttgtatagaa gggcaatacc tcttttgcct gaggtagaaa
     2581 tttttgcaat ggcaggaata gttcccggag gatcgaaggg aaatcttgaa tatgtaaaga
     2641 atgatgtcat ttttacagaa gatattccct tgccgtttca gtatatttat tccgatgctc
     2701 aaaccaacgg aggattgttg attgctgtta tgagcgaaga ggctgaaaat ctattagaat
     2761 ctttgcatga agctggcatt catcaggcaa gccttatagg agaattt
//
LOCUS       DAOORU010000215         2730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_44986_length_2730_cov_3.591402, whole genome shotgun sequence.
ACCESSION   DAOORU010000215 DAOORU010000000
VERSION     DAOORU010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44986_length_2730_cov_3.591402"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2730
                     /locus_tag="PLF20_09820"
     CDS             <1..>2730
                     /locus_tag="PLF20_09820"
                     /inference="COORDINATES: protein motif:HMM:NF025264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HOX81334.1"
                     /translation="VWSNMVWIWVNTAPLITQQPQGISTCEGQNYTLSVTALGDSIHY
                     QWQLEGIDIPGATSNIYTIYNIQYNQTGNYTCYMSTRCGNTMTHQAKVTVNRVPVITQ
                     QPSNVSACEGDFVSIDFGITGDSLHYQWYKNGNPITGASSAALQFPAITISDRGIYRC
                     YIHNTCGEAWTISVNVWVNILPGVVSQPYSQTRCENDSVSFRIIPIGDSISLQWYHNN
                     EPIPGATDSIYQIPVVALADAGTYFCRVISSSCGFYDSDIVTLNVVQKFAINTVTTNI
                     SCNGASDGAIDLTLVNATQPVHYHWSNGATSEDLTGLAPGFYSVYIVDHNNCREKKTI
                     EIGEPDPLAFVHDTTIFSNVLRQGGPGNDMIMAIKTDKNGNTYVTGKFQGTANFQTQT
                     LTSFGLDDIFLAKYNTAGNLSWIRQAGGSLQDQGHDLALDSLGNIYLTGSFQVMAFFG
                     STQILANGNKDIFLAKYDNNGNVLWVKNFGGFFDDVGNSIVATEGGYTWLAGSVQGIA
                     TFENSTVISNGGDDAFVARFNPNGELQWVKHAGGTSEDFAYAITIDIAEDAIITGRFQ
                     STAQFGTSTLVSSGNNDIFLAKYDPIGNVKWVRKAGGTGNDLARAVKTDYSQNIYLSG
                     SIEGNANFGTLNVSSHGLKDAFIAKYTKDGYPQWIRTIGGAGIDNAKDLDIDALGNTY
                     TAGTFRNNVTLGRKTLTSVGSSDIYVAKYNTNGYLVWSQQAGGTGADSAMAIALSHDK
                     NLALGGSFSNEAAFGGFSLISQGGDDAFLTRLNQVAITTPPEIENALCFNGNEGSINL
                     TVGGGTLPYDFFWSTGATTEDLTNIPAGTYWVFINDANNCELDDQFTIESQFPFPNPP
                     IAASVNRDYFCTNDPGNIVLTANGGSGDVLNWYTGSCGGVLIGSGSPLTIPSPT"
BASE COUNT          796 a          580 c          600 g          754 t
ORIGIN      
        1 ggtttggagc aatatggttt ggatttgggt aaatacggca ccccttataa ctcagcaacc
       61 acaaggcata tctacttgtg agggtcaaaa ttatacctta agtgttacag ccctcggcga
      121 ttccatccat tatcaatggc aacttgaagg catcgatatc cctggagcta cctccaatat
      181 ttatacgata tataacattc aatacaatca gacaggaaat tatacctgtt acatgagcac
      241 gcgctgtgga aataccatga cacatcaggc aaaagttacc gtaaatcgcg ttcctgttat
      301 tactcagcaa ccttctaatg taagtgcttg tgaaggcgac tttgtttcta tagattttgg
      361 tataacaggc gattctcttc attaccaatg gtacaaaaat gggaatccca tcacaggagc
      421 aagcagtgct gccttacagt ttccagccat aactataagc gatagaggta tataccgttg
      481 ctatattcac aacacttgtg gtgaagcctg gacaataagt gtaaatgttt gggtaaacat
      541 attgcctggg gttgtatctc aaccatatag ccagactcgc tgcgaaaacg attctgtaag
      601 tttcaggata atcccaattg gagatagcat ttcattgcaa tggtatcata ataacgaacc
      661 cattccggga gcgacagata gcatttatca aattcctgta gtagcccttg ccgatgctgg
      721 aacatacttc tgtcgagtaa tcagtagcag ttgtggattc tacgatagcg atattgttac
      781 tctgaatgta gttcaaaaat ttgccatcaa taccgtaact accaacatca gttgcaatgg
      841 agccagtgat ggagcaatag acctcacgct tgtaaatgcc acacaacctg ttcattatca
      901 ttggtcgaac ggagcaactt cggaagacct caccgggttg gctcctggtt tttatagtgt
      961 ttacattgtt gaccataata attgccgcga aaagaagacc atagaaattg gcgaaccaga
     1021 tcccctggct ttcgttcatg atacgacgat tttcagcaat gtattgcgac aaggtggccc
     1081 gggcaacgat atgatcatgg ctatcaaaac cgataagaat ggaaatactt acgttaccgg
     1141 caaattccaa gggacagcaa atttccagac tcaaacccta accagttttg gactcgacga
     1201 tattttcctc gccaagtata atacagctgg taatttaagc tggattcgcc aggctggagg
     1261 ctctttacag gatcaaggtc atgacctggc tctcgactct cttggaaata tctatctcac
     1321 aggttcattc caggtgatgg ccttctttgg atcgacccaa atcttagcga atgggaacaa
     1381 agatattttc ctggctaaat acgataataa cgggaacgtg ctatgggtca aaaactttgg
     1441 aggattcttc gacgatgtgg gaaattctat agttgccaca gaaggtggtt atacctggct
     1501 tgcaggttct gttcagggaa tagctacctt cgagaattct acggtgataa gtaatggcgg
     1561 tgatgacgca tttgtagctc gtttcaaccc caatggcgaa ctgcaatggg ttaaacatgc
     1621 aggaggaacc tcggaagatt ttgcctatgc aattacaata gatatcgctg aagatgcaat
     1681 cattactgga agatttcaaa gtactgctca atttggaact tcaaccctcg tttcatcggg
     1741 caacaacgat atctttctgg caaaatatga ccccattggc aatgtaaaat gggtgcgtaa
     1801 agccgggggt acaggtaatg accttgctcg cgcagtgaaa acagattatt cacaaaatat
     1861 ttatctctcc ggaagtatcg aaggtaatgc caattttgga acactaaatg tcagttcaca
     1921 tggtttaaaa gatgccttca ttgcaaaata cactaaagat ggatacccac aatggatcag
     1981 aacaattggc ggtgcaggaa tagacaatgc taaggatctg gatatagatg ccttaggcaa
     2041 tacttatact gcaggtacct tccgtaacaa tgttacattg ggcagaaaaa ctttgacaag
     2101 tgttggaagt agcgatattt acgttgccaa atacaacacc aatggctatt tggtctggtc
     2161 gcagcaggcc gggggtacag gtgccgattc ggcgatggct attgcactta gccacgataa
     2221 aaaccttgcc ttgggaggat catttagcaa tgaagctgct tttggaggat tttcccttat
     2281 tagtcagggt ggagatgatg ccttcctgac tcgtctgaat caggttgcca taaccactcc
     2341 tcccgaaatc gaaaatgcac tttgtttcaa cggaaacgaa ggcagcatca accttaccgt
     2401 tggcggtgga acattgcctt acgacttctt ctggtcgaca ggagctacta ccgaagacct
     2461 tactaatatt cctgcaggta cttactgggt atttatcaat gatgccaata actgtgaatt
     2521 agacgatcag tttacgatag aaagtcaatt ccccttcccc aatccaccta ttgctgcctc
     2581 agtgaataga gattatttct gtacaaacga tccgggaaat atcgttctta ccgccaatgg
     2641 tggttctggc gatgtactga attggtatac tggtagttgt ggaggtgtac tgattggctc
     2701 gggttctcct ttgactatac caagcccaac
//
LOCUS       DAOORU010000216         2691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_45853_length_2691_cov_4.677162, whole genome shotgun sequence.
ACCESSION   DAOORU010000216 DAOORU010000000
VERSION     DAOORU010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45853_length_2691_cov_4.677162"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="PLF20_09825"
     CDS             <1..1120
                     /locus_tag="PLF20_09825"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOX81335.1"
                     /translation="PSAMVVDITRGLSVGLLLQSREAASGDCIPYDIANFGYYGLEHK
                     AQHNIEYNDYNIYTAGLVWTELGVNIGRTLKRERFDLWAAGITLKYLWGHSGYFARID
                     NVRYVVLNDSTMDIRNLDAKAGFSLAIDYSNNDFPGPEGYTLGHGFSFDLGITFIKTN
                     RELRRLFPGRLCEARFEPYVFRLGLSLLDIGSIRFSRNAQKHVFDGVSHFWEHARDFT
                     YTTVDEAVNDISNRFYGNPTASLNDTSFTVGLPTAFSIQFDYHLNEKYYVHAMAIQAF
                     PILGEEAVKRANVIYVMPRYETPAFEIGIPLSLYRYRIPRLGFWFRIYSFTLGTDNLG
                     MFLRTGDFNNADIYLSLRIPLRKGKCLKSKGKLPCESF"
     gene            complement(1274..>2691)
                     /locus_tag="PLF20_09830"
     CDS             complement(1274..>2691)
                     /locus_tag="PLF20_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOX81336.1"
                     /translation="IPAYYLPNREVIVAKTQKEAYQIALKQYPDLDELKPEDLKQDED
                     VLDTWFSSWLWPISVFDGIRHPHNPDIEYYYPTNDLVTAPEILFFWVARMIMAGLEYR
                     GEIPFRNVYFTGIVRDKLGRKMSKQLGNSPDPIALMKKYSADGVRVGMLLTSPAGNDL
                     PFDEALCEQGRNFANKIWNALRLLKGWEIDEKIPQPNYSAAACRWFENRFNKSLLIIE
                     DHFGKYRISDALMEAYRLIWDDFCSWLLEIVKPPYQHPMDKITYQLVNGFFDNLLRIL
                     HPFMPFITEEIWHHLQTRSKNDTIMLAPWPDTHEVDETLLNDFDFAREVIVNIRNLRQ
                     NQNIPPKKMIELYYIDSSQSNLKPDFKSIITKLSNLSKIAPLSANMENALTFIVKSTE
                     FYVPAPQNFDLGNERKKLMEKLEYTKRFLSSVNSKLNNEGFRKHASQKVIENELKKKA
                     DAEERIRLLREKLDQLNRHSK"
BASE COUNT          737 a          504 c          575 g          875 t
ORIGIN      
        1 cccctcagca atggttgtag atattaccag gggattgagt gtaggtttac ttctccaatc
       61 acgcgaagct gcttcgggcg actgcattcc gtacgacatc gctaattttg gatattatgg
      121 acttgaacat aaggctcaac ataatattga atacaacgat tacaatattt ataccgccgg
      181 gcttgtgtgg acagaacttg gggtgaatat tggccgcacc ttaaaaaggg aaaggtttga
      241 tttatgggcg gccggcatca cattgaaata tctatggggt cattcaggtt attttgcaag
      301 aattgacaat gtccgctatg tggtattgaa cgattctacc atggatattc gcaaccttga
      361 cgccaaagct ggtttttcac tggctattga ctatagcaat aatgatttcc ctggtccgga
      421 aggttatact cttggtcatg gattcagctt cgaccttgga ataactttta tcaaaacaaa
      481 tagggaattg aggagacttt tcccgggcag gctttgtgag gcacgtttcg agccttatgt
      541 tttcagattg ggcttgtcat tgcttgatat tgggagtatt cgcttttcac gaaatgccca
      601 gaaacatgta ttcgatggag taagccactt ttgggaacat gcccgggact tcacttatac
      661 tacggttgat gaagcggtaa atgacataag caaccgcttc tatggaaatc caactgcttc
      721 gttgaacgat acttcattta ctgtgggctt gccaacagcc tttagtattc agttcgatta
      781 tcatttgaat gagaaatatt atgttcatgc catggcgatt caagctttcc ctatattggg
      841 tgaagaggcc gttaaacgtg caaatgtcat ttatgttatg ccaagatatg aaactccggc
      901 atttgaaatt ggaattcctc tgagtctcta tcgttatcga attccacggt tgggcttttg
      961 gttccggata tattccttta cccttggtac agataatctg ggtatgtttc tgcgaacggg
     1021 cgacttcaac aatgccgata tttatctatc cttgagaatt cccttgagaa agggaaaatg
     1081 tctgaaatcg aaaggaaaat tgccttgcga aagtttttga ttgctgtaaa ccgagaatgc
     1141 gtaaggtttc gaatcatgcc ccttctgaaa taaacgattc tatttttcta aaaaaatgtt
     1201 tcttcttaca atccattttt ttaacggctt ttgtatagcg taagattctt ccaagatatt
     1261 cattgtattt tctttatttg gaatgtctgt tcaactgatc gagtttttct ctcaatagtc
     1321 gaattcgttc ttccgcatca gccttctttt ttagctcgtt ttcgatcact ttttgtgaag
     1381 cgtgtttcct gaaaccttca ttatttaatt ttgaattcac tgatgaaaga aatcttttgg
     1441 tatactcgag tttttccatt aatttttttc tttcgtttcc cagatcgaaa ttttgaggag
     1501 caggaacata aaattctgtt gattttacaa taaatgtgag cgcattctcc atattagccg
     1561 aaagaggtgc aattttcgat agattggaaa gtttggtaat gatggattta aagtcgggtt
     1621 tcagattaga ttggcttgaa tcgatgtaat acagttcgat catctttttt ggaggaatat
     1681 tctgattttg acgcagatta cggatattga cgataacttc acgagcaaaa tcgaaatcat
     1741 tcagcaaagt ttcatctact tcatgagtat ctggccatgg agcaagcata atagtatcgt
     1801 ttttagaacg ggtttggaga tggtgccata tttcttcggt aatgaagggc ataaatggat
     1861 gtagaatccg tagtaagttg tcgaaaaagc cattcacaag ctgataggta attttatcca
     1921 ttggatgttg atagggaggt ttgactattt cgagcaacca tgaacaaaaa tcgtcccata
     1981 tcaggcgata agcttccatg agagcgtctg aaatgcggta ttttccgaag tggtcttcga
     2041 taatgagtaa tgatttgttg aacctattct cgaaccatcg gcacgcagcg gccgaatagt
     2101 tgggctgtgg aattttttca tctatttccc aacctttcaa caagcgtaaa gcattccaga
     2161 ttttattagc gaaatttcgg ccttgttcac atagtgcttc atcgaaaggc agatcattgc
     2221 ctgcgggtga agtaagcaac attcctaccc taacgccatc ggcactgtat tttttcatta
     2281 gtgcgatagg atcaggcgaa ttgcccagtt gtttcgacat ttttctacct aatttgtcgc
     2341 gaacaatgcc tgtaaaatag acgttacgaa agggaatttc gcctctatat tccaatccag
     2401 ccataatcat acgtgctacc cagaaaaata gaatctccgg agccgtaacc aaatcgttgg
     2461 tcggataata atattcgatg tcgggattat gaggatggcg tatgccatcg aataccgaga
     2521 taggccaaag ccacgacgaa aaccaagtgt cgagtacgtc ctcatcctgt ttcagatctt
     2581 ctggtttcaa ttcatcgaga tcaggatatt gtttcagggc aatttgatag gcttcttttt
     2641 gtgttttggc aacaataact tctcgattgg gtaaataata ggcaggaata c
//
LOCUS       DAOORU010000217         2651 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_46810_length_2651_cov_4.362866, whole genome shotgun sequence.
ACCESSION   DAOORU010000217 DAOORU010000000
VERSION     DAOORU010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46810_length_2651_cov_4.362866"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..656)
                     /locus_tag="PLF20_09835"
     CDS             complement(129..656)
                     /locus_tag="PLF20_09835"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOX81337.1"
                     /translation="MDNLLRWYAVYTRSRYEKKSAFLLNEQGIEAYVPLRRVLRQWSD
                     RRKLVFEPVIHSYVFVKANKMLYEKVLGIEGVVRYIYFGGKPAPIPDRQIETMKTVVG
                     TDLEIECTPDCLKPGMHVIVTEGPLAGMEGELVQFRGKQKVAVRIQAIQQVIMVTLSP
                     LILQPVQNTTPAIAL"
     gene            complement(748..1290)
                     /gene="ispF"
                     /locus_tag="PLF20_09840"
     CDS             complement(748..1290)
                     /gene="ispF"
                     /locus_tag="PLF20_09840"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018107646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOX81338.1"
                     /translation="MMLPRIGFGYDVHRLSKGYELWLGGLKLDFPLGAVGHSDADVLL
                     HAICDALLGAAALHDIGYYFPDNDPQYKGISSLLLLKEVAKLLQHHEYQIGNIDSTII
                     LEQPKIAPYIPQMQQNIAQTLQIPITSISIKATTSEGLGYEGRQEGISAFAVALVFSK
                     NLYINNFPEENYNFSKFNKF"
     gene            1947..>2651
                     /locus_tag="PLF20_09845"
     CDS             1947..>2651
                     /locus_tag="PLF20_09845"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HOX81339.1"
                     /translation="MRKKFCIKYLNLASIKIYRLAILSVLVISLVEMSLAQGPSVPIL
                     NLAKERGLSNGAVNCINMDSEGYLWLGTWNGLNRWDGSQIVSYLPGSSPHSLHNHVVR
                     RIIPLKKGGLWLMTNKGISKYDNQLDEFTAYFDNLPGNLNYETDLSMTLAEDSVLFVS
                     VKGRGLYQYISDEKNFRLRDDIHDSLEILQSLVSIQSSVNGLVLLTNNGKVWRLKGED
                     LYLIGKFHTNAPLVSTF"
BASE COUNT          844 a          425 c          625 g          757 t
ORIGIN      
        1 aactaccaac taccaactac caactaccaa ctaccaacta ccaactacca actacagctt
       61 cgccctttca gtcccatacg caccattgga gacctaagcg aaaagaagca gaagatgttc
      121 cagtaaagct acaaagcaat agctggagta gtattctgga caggttgcag gataagaggc
      181 gaaagggtta ccatgattac ctgttgaata gcttgtatcc gaacggctac tttctgcttt
      241 ccgcgaaact gcaccaactc gccttccatt ccggccagcg gaccttcggt tacaattacg
      301 tgcatacccg gtttcaaaca atcgggcgta cattctatct cgaggtcggt gccgactacc
      361 gttttcattg tctcaatctg acgatcgggg ataggagcag gcttgcctcc gaaataaata
      421 taacgtacaa cgccttcgat gcctaatact ttctcgtaaa gcattttatt ggcttttacg
      481 aaaacatacg aatggataac aggttcgaaa accagcttgc gacggtcgct ccattgcctg
      541 agcacacgcc ttagaggcac ataggcttcg ataccctgtt cattcaacag aaaagctgac
      601 tttttctcgt acctggaacg agtgtaaacc gcataccatc ttaacaagtt atccattcaa
      661 ttatgagttt attaggatga aaagagctgc ttgcagtttt gagtaacaaa attactcaaa
      721 gattttgaat ttgcaaattt ttttatgtta aaatttgtta aattttgaaa aattataatt
      781 ttcttcaggg aaattgttga tatataaatt cttagaaaat acgagtgcga cggcaaaagc
      841 ggaaatacct tcttgtcggc cttcgtatcc taagccttct gatgtagtgg ccttaataga
      901 aatagaagta atagggattt ggagggtttg agcgatattt tgttgcattt gagggatata
      961 aggagcgatt ttaggttgct ctaaaattat ggtggagtcg atattaccga tttgatattc
     1021 atgatgttgg aggagtttgg ctacttcttt taaaagcagc agtgatgaga ttcctttata
     1081 ttggggatca ttatcgggga aatagtagcc aatatcatga agagctgccg caccaagcaa
     1141 ggcgtcgcag atagcgtgaa gaagaacgtc ggcatcggaa tgaccaactg ctcccagcgg
     1201 aaagtcgagt tttaagccgc ctaaccaaag ctcgtaacct ttagaaagcc ggtgaacatc
     1261 gtaaccaaat ccaatgcgtg gaagcatgat attagattat aatcgttatg aagggcaaaa
     1321 gtaaaaaaat atttaaaaca cgggaaaagg gaggaagggg aaaagggaga atggagacgg
     1381 gagactggga gacgaagtga atggagatta gtggatggtg gttagtgagt aaaaagtata
     1441 aagttaaaag tttataaagt taaaaaggga ggaagggaga aatggtggat agtggatagt
     1501 ggttggtggt tagtaagtaa aagttaaaag tttataaagt taaaaaggaa aaatagagga
     1561 agggagaaat ggtggatagt ggatagtgga tagtggatag tggatagtgg atagtggttg
     1621 gtggttagta agtaaaagtt aaaagtttat aaagttaaaa aggaaaaaga gaggaaggga
     1681 ggaaagaaga aatggtggaa aggatgaaag gagatgggga gatgagaagt taggaaactg
     1741 gagttttgaa tttgaaaaat tatttggaat ttgtaatttg gagttttttg attatcagtt
     1801 gttataagat aatatttttt tcccatttac ttacaactaa taacaaatca ccagctgcca
     1861 tctgccaatc actaatcagc agcctccagc caccattctt cttgtctcaa aaatacttaa
     1921 aaatatccct aattttgttg tttgtcatgc ggaagaagtt ttgcataaaa tatctgaatc
     1981 tggcgagcat caagatttat cgtttagcga ttcttagtgt tttggtcata agccttgtag
     2041 aaatgagtct tgcacagggc cccagtgtgc ctattttgaa tttggctaag gaaagggggt
     2101 tatcaaatgg ggcggtcaat tgcattaata tggattcaga aggatatctt tggcttggaa
     2161 catggaatgg gcttaatcgc tgggatggta gccagatagt aagttatttg cccgggagct
     2221 caccgcattc acttcacaac cacgttgttc gcaggattat tcctttgaaa aaaggtggtt
     2281 tgtggcttat gaccaataaa ggaatttcga aatatgataa tcaactggac gagtttacgg
     2341 cctatttcga taatcttcct ggaaacttaa attatgagac tgacttgagt atgacccttg
     2401 cagaagattc tgttcttttt gtttcggtaa aagggagagg attatatcag tatatttctg
     2461 atgaaaagaa ttttaggttg agagatgata ttcacgattc gcttgaaata ctccagagcc
     2521 ttgtaagtat tcaaagttcg gtaaatggtt tagttttact gacaaacaat gggaaagtat
     2581 ggcgcttgaa aggggaggat ctatatctta taggcaaatt tcatacgaat gctcccttgg
     2641 tatctacctt t
//
LOCUS       DAOORU010000218         2586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_48453_length_2586_cov_5.963651, whole genome shotgun sequence.
ACCESSION   DAOORU010000218 DAOORU010000000
VERSION     DAOORU010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48453_length_2586_cov_5.963651"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1801)
                     /gene="dnaK"
                     /locus_tag="PLF20_09850"
     CDS             complement(<1..1801)
                     /gene="dnaK"
                     /locus_tag="PLF20_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOX81340.1"
                     /translation="MSKIIGIDLGTTNSCVAVMEGNEPVVIPNSEGRRTTPSVVAFTE
                     NGERKVGDPAKRQAITNPKRTVFSIKRMMGETYDRVLKEIEQVPYKVVRGDNNTPRIV
                     IDDRQYSPQEISAMILQKMKKTAEDYLGQEVTEAVITVPAYFSDSQRQATKEAGEIAG
                     LKVRRIINEPTAAALAYGLDKKHKDLKVAVYDLGGGTFDISILELGDGVFEVKSTNGD
                     THLGGDDFDQEIINWLADEFQKEEGVDLRKDPMALQRLKEAAEKAKIELSSSLETEIN
                     LPYIMPVDGIPKHLVKKLTRAKLEQLIDHLVQRTVEPCQKALRDAGLKPSDIDEVILV
                     GGSTRIPMVQKLVENIFGKTPSKGVNPDEVVAIGAAIQGGVLTGEVKDVLLLDITPLS
                     LGIETLGGVMTKLIEANTTIPTRKSEIFSTAADNQTSVEIHVLQGERPLAKDNKTIGR
                     FYLDGIPPAPRGVPQIEVTFDIDANGILNVSAKDKATGKSQQIRIEASTGLTEEEIKR
                     MKAEAEANAEADRRAKEEIEKVNQADNLIFQTEKQLKEYGDKIPADKKANIENALNEL
                     KEAHKQRNIAAIDSAMTRMNQAWQAASEEMYKAT"
     gene            complement(2042..>2586)
                     /locus_tag="PLF20_09855"
     CDS             complement(2042..>2586)
                     /locus_tag="PLF20_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81341.1"
                     /translation="ISRPLYNPSKPFLKILNTDGQNTIKWRAAVNGRRYQVIYRFYYK
                     ESPNPGDTVIKTTDYHLGVIKSQYTTGSEDMDVSFMGTSFYKYLGSSIPANPNVKRIA
                     YQIELIIAVIPEEFNTYLEVNEPSSSIVQDRPEYTNIENGYGLFSSRYIKSRLYFVDS
                     EMAAGIKTYFQTIDGSFIVP"
BASE COUNT          632 a          560 c          526 g          868 t
ORIGIN      
        1 gtgtagcttt gtacatttct tcgcttgcag cttgccatgc ctgattcatc cgagtcatag
       61 ccgagtcgat agctgctata ttacgctgtt tatgagcttc cttcagttcg ttgagagcat
      121 tttcgatatt ggctttctta tcggccggaa tcttatcgcc atattctttc aattgttttt
      181 cggtttgaaa gatgaggtta tcagcctgat tgaccttttc tatttcttct tttgctctgc
      241 ggtcggcttc ggcattagct tcagcttcgg ctttcattcg tttgatttct tcttccgtga
      301 ggcccgtaga agcttcgata cgaatctgtt gcgacttgcc tgtggcttta tctttagccg
      361 aaacattcaa aatgccgttg gcatctatat cgaaagttac ttcgatttga ggaactccac
      421 gtggcgcagg tggaatgcca tcgagataga aacggccaat ggtcttgttg tcttttgcta
      481 aaggacgttc accttgtaaa acatgaatct ctacactggt ctgattatcg gcagctgtgg
      541 agaatatttc cgattttctg gttggaatag tggtattggc ttcaatcaat tttgtcatta
      601 ctccacccaa agtttctata cccagcgaaa gaggagtaat atcgagcaac aggacgtctt
      661 taacttcgcc tgtaagaacg cccccctgaa ttgcagctcc tattgccacc acttcatcgg
      721 ggttgacgcc tttcgaggga gttttaccaa aaatattttc taccagtttt tgtaccatag
      781 gaattcgcgt ggaacctccc accaaaataa cttcatcgat atcggagggt tttaatccgg
      841 catctctcag ggctttctgg cagggttcta cggtacgttg taccaaatga tcgataagtt
      901 gttcgagttt ggctcgtgtc aattttttta ccagatgttt gggaattcca tccactggca
      961 taatataagg caaattgatt tcggtttcga gggagctcga aagttctatt ttggcttttt
     1021 cggcggcttc cttcaaacgc tgaagggcca ttgggtcttt tctcaggtcg acgccttctt
     1081 ctttctggaa ttcatcggcc aaccagttga taatttcctg gtcgaaatcg tctccaccaa
     1141 ggtgtgtatc gccgttggtt gattttactt caaagacgcc atcgcccaat tcgagtatgg
     1201 atatatcgaa agttccacct ccaaggtcgt aaactgccac cttcaggtct ttgtgttttt
     1261 tatcgagacc ataagccaga gctgcggctg taggttcatt gataatacgt ctcaccttga
     1321 ggcctgcgat ttctcctgct tcttttgtag cttgacgctg tgaatcgcta aagtaagctg
     1381 gcacagtaat gacagcttcg gtaacctctt gtccaaggta atcctcagcc gtcttcttca
     1441 ttttttgaag aatcatggca gaaatctcct gaggtgaata ctgccggtca tctataacaa
     1501 tgcgaggggt attgttatca cccctgacaa ctttataagg cacctgttct atttctttca
     1561 gaacgcggtc gtaagtttcg cccatcatac gtttaataga gaaaactgtc cttttgggat
     1621 tggtgattgc ctgacgtttt gccggatccc ctaccttacg ctcgccattt tcggtgaagg
     1681 ctacaaccga tggagtagta cgacgtcctt cactgttagg tattactacc ggctcattac
     1741 cttccatcac agcaacacaa gagtttgtcg tcccaaggtc tattcctatt attttactca
     1801 ttttcaatac gaattttaga gttattgaat ttacttttga atcgcttacc cctctcgcaa
     1861 tctatatgcc aaagcaatct tctttttata aaatgcacaa agtactgcct tctttcaccg
     1921 attttgtggt aaaaaaatta ttattcgatt gattgttaat atgaaataag aatgatatcg
     1981 aaactgttcg ctctgtcatt ctggcagttt gtttaagagg tttttattcg aaatggaaaa
     2041 atcaggggac aataaaactg ccatcaatag tttggaagta cgtctttatc cctgctgcca
     2101 tttcggaatc tacaaaatat aaacgtgatt ttatataacg gcttgaaaat aaaccatatc
     2161 cattttcaat attggtatat tcgggcctgt cttgaacgat actcgaactg ggttcgttca
     2221 cttccagata ggtgttgaat tcttcgggaa ttacggcaat aatcagctct atctgatatg
     2281 ctatcctttt cacattggga ttggcaggaa tagaggatcc taaatattta tagaaagatg
     2341 tacccatgaa actaacgtcc atatcttcac ttcctgtagt atattgactt ttaataactc
     2401 cgagatgata gtcggtagtt tttattaccg tatcgcctgg atttgggctt tctttgtaat
     2461 aaaacctgta aatcacctga tatcttctcc cattcactgc agctctccat ttgatggtgt
     2521 tctggccatc ggtattcaga attttcaaaa agggtttgct gggattatac aaaggacgcg
     2581 aaatgt
//
LOCUS       DAOORU010000219         2580 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_48618_length_2580_cov_2.775842, whole genome shotgun sequence.
ACCESSION   DAOORU010000219 DAOORU010000000
VERSION     DAOORU010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48618_length_2580_cov_2.775842"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLF20_09860"
     CDS             <1..539
                     /locus_tag="PLF20_09860"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HOX81342.1"
                     /translation="TIEAQTSPQIVVRATIFENDTIPLINLNPVTIYGLPVFKNKKDQ
                     RQWEKLVRNVKKVYPYARLAGIKFQEYENLILTSKSEKERKKYVNRLEKELTEQYSDE
                     LEKLTFTQGKILFKLLDRETGNSSYELVKELKGKFMAFFWHSFARIFGYNLKEKYDPY
                     GKDATIEQIVVLIENGAL"
     gene            complement(546..1958)
                     /locus_tag="PLF20_09865"
     CDS             complement(546..1958)
                     /locus_tag="PLF20_09865"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOX81343.1"
                     /translation="MNKNLQIAKYVVADYLTALLAWSLFFFYRKYYVDPYFLEHYTVV
                     FDDPKFFYGIIGIPFFWLILYAISGQYRKIYRKSRLKELGHTLIITLIGVLIIFFVLI
                     LDDVIRSYKSYYQSFLVLFVLHFSFTYSARFLLTTITNNKIHRRKIGFNTVMVGCNCN
                     ALSIYQEIENQPLSPGNKFVGFVDAGVSEKNLLDGYMPHLGSYKDLKRIIDEYKIEEV
                     IIAFERSEKDTVERVITELEDANVVIKIAPIMQDILLGSVRTSSIYSPLIEISTDLMP
                     AWQMVLKRAMDIVVSLIAIIVLSPVFLFTAIGVKLSSPGPILYSQPRVGLRGKTFKMV
                     KFRSMYQDAESNGPQLSSKNDPRVTPFGRIMRQYRLDEIPQFFTVLKGDMSIVGPRPE
                     RSYYIEQIIQKMPHYKLLLKVKPGITSWGQVKFGYAENLDQMTERAKFDLIYLDNMSI
                     AMDIKILVYTIMIVLQGRGK"
     gene            complement(1962..>2580)
                     /locus_tag="PLF20_09870"
     CDS             complement(1962..>2580)
                     /locus_tag="PLF20_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81344.1"
                     /translation="QLRAYYPLYNQIRREIFLSPDSVTIIKGNLINGKNTIYTTYKDL
                     VQFSWSENFEDENLALDTTKKSNVNFMLTPEDDPATFEGLHSGKIILTLTDTIFEAMT
                     SEAISLPRNGSPVFLEMNYRCTNTFTFGIIALGSDQYIQSPILDLNPSASWNKIYINL
                     TPNVSSSDDALKFKLFWGAMLDIGQEQTEIYFDNMKLVYLNNQSK"
BASE COUNT          837 a          474 c          501 g          768 t
ORIGIN      
        1 tgaccattga agctcaaact tcacctcaaa tagtggtcag ggctactata ttcgagaatg
       61 atactatccc tttgataaac ctcaaccctg ttacaatcta tggtttaccc gtattcaaaa
      121 acaagaagga tcagcgtcaa tgggaaaaat tggtacgtaa tgtgaaaaaa gtgtatcctt
      181 atgcacgatt ggcaggcatt aaatttcagg aatacgaaaa tctcattttg acctctaaat
      241 cggaaaagga acggaaaaaa tatgtcaatc gtttagaaaa agagctaacc gaacaatatt
      301 ccgacgaatt ggaaaaactc acctttacac aagggaagat tctatttaaa ctattagata
      361 gagaaacggg taattcttcg tatgaactcg taaaagaact caagggaaaa tttatggctt
      421 ttttctggca ttcatttgcc cggatttttg gatataattt gaaagaaaaa tacgaccctt
      481 acggaaaaga tgccactatc gaacagatcg tggttctaat cgaaaacggt gctctttgag
      541 agcaattact ttcctcttcc ctgtaaaaca atcatgatgg tatagacgag tatcttaata
      601 tccatggcaa ttgacatatt atccagatat atgagatcga attttgccct ttcggtcatc
      661 tgatcgaggt tttcggcata accaaatttt acctgtcccc aactcgttat tcctggtttt
      721 actttgagta acagtttgta atgaggcatt ttttggatga tctgttcgat gtaataagaa
      781 cgttcgggcc tcggacctac tatggacata tcacctttca gtactgtaaa aaactgaggg
      841 atttcgtcca atcggtattg ccgcatgatt cttccaaaag gtgttactcg cggatcgttt
      901 ttcgaggata attgtggacc attgctttcg gcatcttgat acatagaacg gaatttgacc
      961 attttaaatg ttttccctct cagtcctact cgtggttggc tatagagtat aggacccggc
     1021 gacgaaagtt ttactccaat ggctgtgaat aaaaatacgg gagaaagaac aataatcgca
     1081 atcaatgaaa cgacaatatc catggctcgt ttcaatacca tttgccatgc aggcatcaaa
     1141 tcggtcgaaa tttcaatcaa gggtgaataa atggaggagg tacgaaccga ccccagaaga
     1201 atatcctgca tgatgggagc aatctttatc accacattgg catcttccag ttcggtaatt
     1261 acccgttcga cagtatcttt ttccgatcgc tcgaatgcaa tgattacttc ctcaatctta
     1321 tattcatcga ttattctttt cagatcctta tagcttccca aatgaggcat ataaccatcg
     1381 agtaaattct tttcagaaac ccccgcatcg acaaatccaa caaatttgtt gcctggcgac
     1441 aaaggctggt tttcgatttc ctgatatatg gataaggcat tgcaattgca gcccaccata
     1501 accgtattga atccaatctt tcgtcgatga atcttgttgt tagtaatagt agttagtaaa
     1561 aatcgggccg aataagtaaa tgaaaaatgc aaaacgaata aaactaaaaa tgattgataa
     1621 taggatttat aagaacgaat tacatcatca agaatcaaaa caaagaaaat aattagaacc
     1681 cctatcaggg taatgatcaa agtatgaccc aattccttca acctcgattt acgataaatt
     1741 ttacgatatt ggcctgatat agcataaaga attagccaga aaaagggaat tccaataatg
     1801 ccgtaaaaga atttgggatc atcgaaaacc acggtataat gttcgagaaa ataaggatcg
     1861 acataatatt tgcggtaaaa gaaaaacaag ctccatgcta gtagtgcagt aagataatca
     1921 gcaacaacat atttagcaat ttgtaaattt ttattcataa actacttcga ctgattattt
     1981 aaataaacca atttcatatt atcgaaatat atctcggttt gctcctgccc aatatcgagc
     2041 atggctcccc agaagagttt aaacttaagg gcatcatcag agctactaac gtttggagtg
     2101 aggtttatat aaattttatt ccaacttgca gatgggttca gatcgaggat tggagattga
     2161 atatattgat cgcttcccag tgcaatgatt ccgaaagtaa atgtgtttgt gcaacgatag
     2221 ttcatttcga ggaacactgg tgagccatta cggggcagac ttatggcttc ggaagtcatg
     2281 gcttcgaaaa tggtatcggt caatgtaaga ataattttcc ctgaatgtaa gccttcgaat
     2341 gtggctggat catcttcggg agtgagcata aaattgacat tgcttttttt tgtagtatca
     2401 agggcaaggt tttcatcttc gaaattttca ctccaggaaa attgaaccaa atccttgtat
     2461 gtggtataga ttgtattttt cccgttgatt aaatttcctt ttatgatggt aaccgaatca
     2521 ggggaaagaa agatttcgcg gcgtatttga ttgtagaggg ggtaataagc tcgcagttgc
//
LOCUS       DAOORU010000220         2458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_52074_length_2458_cov_5.560965, whole genome shotgun sequence.
ACCESSION   DAOORU010000220 DAOORU010000000
VERSION     DAOORU010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52074_length_2458_cov_5.560965"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..2101
                     /locus_tag="PLF20_09875"
     CDS             92..2101
                     /locus_tag="PLF20_09875"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009229794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOX81345.1"
                     /translation="MDNTEFKNQILQGIPDELPLPKPYDPNINHAPKRKDILTKEEKK
                     LAVRNALRYFPAKHHAVLAKEFAEELKHYGRIYMYRFRPDYRIYARPIWEYPAQTPQA
                     AAIMLMIQNNLDEAVAQHPHELIIYGGNGAIFQNWAQYLLTMKYLATMTDHQTLVMYS
                     GHPLGLFPSHPEAPRVVVTNGMVVPNYSSPDHWEKFNALGVSQYGQMTAGSYMYIGPQ
                     GIVHGTTITIMNASRKISRKDSASEMKLFVSSGLGGMSGAQPKAGNIAGVVSLVAEIN
                     PKAAYKRFNQGWVDEVIDDVEKAIRTALHWKEMGKPHSIAYLGNVVDLWERLAIDDIH
                     VDIGSDQTSLHNPFAGGYYPVGLSFEESQEMMVSQPEVFKEKVKASLRKQVAAINKIA
                     ANGMYFFDYGNAFLLESGRAGADIFNASGNFIYPSYVQDIMGLLYFDYGFGPFRWVCT
                     SGNPADLDKTDQIAGDIFEKMLTDAPVEIQQQLKDNLLWIRQAKTNNLVVGSQARILY
                     ADAEGRIRISEAFNQAIARGEISAAIVLGRDHHDVSGTDSPFRETSNIYDGSSFTADM
                     AVQNMAGDSFRGATWVSLHNGGGVGWGEAINGGFGLVLDGTAEAQHRLRSMLFWDVNN
                     GIARRSWARNQGAMLTIQKTMQHEPHLKVTLPNLVDDNLIETLDF"
     gene            2132..>2458
                     /locus_tag="PLF20_09880"
     CDS             2132..>2458
                     /locus_tag="PLF20_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOX81346.1"
                     /translation="MKIAGIIPARYASTRFPGKPLADIKGKSMIMRVYEQAAKVQKLD
                     EVVVATDDQRIFKHVIENGGKAVMTSPDHPSGTSRCAEAIKLISRNDHFDSVINIQGD
                     EPFIRPE"
BASE COUNT          685 a          520 c          566 g          687 t
ORIGIN      
        1 cttttatcgg gcatcctgag tcaagtaaaa catataccgt aatttaacga atttttttat
       61 ttttacgatt cgaatcaatt gcattatcat tatggataac accgaattca agaatcaaat
      121 tctgcagggt attccggatg aactcccttt gcccaaacct tacgatccta acataaatca
      181 tgctccaaag cgtaaggata tattaaccaa ggaagaaaag aaattagccg ttcgtaatgc
      241 gctacgatat tttccagcaa agcatcatgc tgttctggcg aaagaatttg ccgaagaatt
      301 gaagcattat ggccgaattt acatgtaccg ttttcgtcct gattatagaa tttatgctcg
      361 ccctatctgg gaataccctg ctcaaactcc tcaggctgcc gccatcatgc tgatgattca
      421 gaacaatttg gatgaagccg tagcccagca tcctcacgaa ttgatcatct acggaggcaa
      481 tggagctatt tttcagaatt gggcacaata tctcctcacc atgaaatatc tggctactat
      541 gaccgatcat cagactttgg tgatgtattc gggacatcct ttgggattgt tcccttctca
      601 tcctgaagca cctcgcgtag tagttaccaa tggaatggtt gtgcccaact attccagtcc
      661 tgaccattgg gaaaaattca atgcacttgg agtatcccaa tatgggcaga tgaccgccgg
      721 ttcatacatg tatattggtc ctcagggaat tgtgcatggt acaacgatta ccattatgaa
      781 tgccagccgg aaaatatctc gtaaagattc ggcttcagaa atgaaattat ttgtttcgag
      841 tggtttggga ggcatgtcgg gtgcgcaacc aaaagcaggg aatattgcag gcgtagtgag
      901 cctggtggcc gaaatcaatc ccaaggcagc ttacaagcgt ttcaaccaag gctgggtaga
      961 cgaagtgatc gatgatgtag aaaaggctat tcgaactgct ttgcattgga aagaaatggg
     1021 taagcctcat tcgattgctt atctggggaa tgtggtcgat ttatgggaaa ggcttgccat
     1081 cgatgatatt cacgtagata ttggttccga tcaaacttct ttgcataacc cctttgcagg
     1141 tggttattat ccggtcggtc tttctttcga agaatcacag gaaatgatgg ttagccagcc
     1201 cgaagttttc aaggaaaagg taaaagcgtc actgcgcaag caagtggcag ccatcaacaa
     1261 aattgcagct aatggaatgt atttcttcga ttatggcaat gcattcttat tggaatcggg
     1321 cagggcagga gccgatatct tcaatgcaag tggaaatttt atttatcctt cgtatgttca
     1381 agatattatg gggctattgt atttcgatta tggttttggt ccgtttcgtt gggtgtgcac
     1441 cagtggcaat ccggccgatc tggacaaaac cgatcagatt gccggagata ttttcgaaaa
     1501 aatgctcaca gatgctcctg tcgagattca gcaacagttg aaggacaatc ttttatggat
     1561 acgtcaagca aaaaccaata atcttgtggt aggttctcag gccaggatct tatatgccga
     1621 tgccgaaggg cgcattcgta tttcggaagc cttcaatcag gccatcgcca gaggagaaat
     1681 ttcggctgcc atagttctag gacgagatca tcacgatgtt tcgggtactg attctccctt
     1741 cagggaaact tcgaacatct atgatggttc atcatttacg gccgatatgg ctgtacagaa
     1801 tatggcagga gattctttca gaggtgccac ttgggtttcg cttcacaatg gcggaggtgt
     1861 tggctggggt gaagccatta atggaggttt tggccttgtt ctcgatggaa cggctgaagc
     1921 ccagcaccgt cttagatcca tgctcttctg ggatgtaaac aatggcattg cacggcgttc
     1981 atgggcacgt aaccagggcg ctatgctcac tatccaaaaa accatgcagc atgaacccca
     2041 cttgaaagtt acgcttccta atctggtcga tgataacctg atcgaaactc tcgatttttg
     2101 aaatttttct tttactctca agctacaacc tatgaagata gcaggcatca ttcctgcaag
     2161 atatgcttcg acaagatttc cgggtaaacc tctggctgat atcaaaggca aaagcatgat
     2221 aatgcgagtt tatgaacaag ctgcaaaagt gcaaaaactc gatgaggttg ttgtggctac
     2281 cgacgatcaa cgcattttca aacatgtaat cgaaaacggt ggcaaggccg tgatgacttc
     2341 tcctgatcat cccagtggaa cctctcgctg tgccgaagca atcaaactta tcagtcggaa
     2401 tgatcacttc gattcagtaa taaatattca gggtgatgag ccttttatac ggcccgaa
//
LOCUS       DAOORU010000221         2394 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_54097_length_2394_cov_5.909363, whole genome shotgun sequence.
ACCESSION   DAOORU010000221 DAOORU010000000
VERSION     DAOORU010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54097_length_2394_cov_5.909363"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..1502)
                     /gene="serS"
                     /locus_tag="PLF20_09885"
     CDS             complement(231..1502)
                     /gene="serS"
                     /locus_tag="PLF20_09885"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018029164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOX81347.1"
                     /translation="MIPLQIIREQPDWVIQRLAVKNFDANSLIYQILELDWKRKSTQF
                     ESDERSAEYRKLTQEIGSLIKAGQAGKANDRKNEVAKIKEEVRNLQERLDEISRQMNE
                     LLVRLPNIPDERVSPGISPADNEIVYSEGSIPQLPPEALCHWDLAVKYQIISFEIGSK
                     VTGAGFPFYKDKGAKLQRSLISFFLDEATKAGYTEYEPPLLVNEDSAFATGQLPDKEG
                     QMYFVPQDNFYLIPTAEVPLTNVYRNEFLKHTDFPIKLCAYSACFRREAGSYGKDVRG
                     LNRLHQFDKVEIVQLSLPENSFQILEEMTEHVAHLLRKLELPFRKVKLCGGDMSFTSA
                     LTYDFEVFSAAQQKWLEVSSVSNFTEFQANRMKLRFKDKNGKTRLAHTLNGSALALPR
                     IMAALLENNQSSQGIHIPEALIPYTGFDSIP"
     gene            complement(1605..1874)
                     /gene="rpmA"
                     /locus_tag="PLF20_09890"
     CDS             complement(1605..1874)
                     /gene="rpmA"
                     /locus_tag="PLF20_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOX81348.1"
                     /translation="MAHKKGAGSSQNGRESESKRLGVKIFGGQFAKAGQIIVRQRGTI
                     HHPGINVGLGKDHTLFALSDGIVEFHRRKNNKSYVSVFPDNKKEN"
     gene            complement(1895..2209)
                     /gene="rplU"
                     /locus_tag="PLF20_09895"
     CDS             complement(1895..2209)
                     /gene="rplU"
                     /locus_tag="PLF20_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HOX81349.1"
                     /translation="MYAIVEIAGQQIKVETKREYYVNRLQGDEATQVIFENILLIDDD
                     STIRVGRPRVEGARVKATISEHIKSDKVLIFKKKRRKGYQKLNGHRQPLSKIIIEEIS
                     VA"
BASE COUNT          679 a          498 c          453 g          764 t
ORIGIN      
        1 tatctcattg tgtgattgtt gtaaaaatgt ctcttaaaat gtgtcgaagt cagggaatcc
       61 ggggcaataa cgcaagactc ttatcgaaaa tatataaaat gtatagaaat ccctctatat
      121 cattatcttt ttcggaatat tcaataaatt cgaatatgct ccagaattcc aattcaatat
      181 aaaaagaggt agaaggccgt attcggtggg tcaggttcgg caatgctggt tcagggaatt
      241 gagtcaaaac ccgtataagg aattaaggct tcagggatat gaataccttg ggatgactga
      301 ttattttcga gcaaggcagc catgatacga ggcaatgcca aggcacttcc gttcagggtg
      361 tgagccaggc gggtttttcc attcttatcc ttaaaacgca gcttcattcg attggcttga
      421 aattcggtga aattcgacac cgagcttact tctaaccatt tttgttgagc cgccgaaaaa
      481 acctcgaaat cataagttaa ggccgaggta aaactcatat ctcctccaca tagttttact
      541 tttcggaaag ggagctcgag tttgcgtagc aaatgggcta catgctctgt catctcttcg
      601 agaatctgaa aggaattttc gggtaatgaa agttgtacta tttccacttt atcgaattgg
      661 tgaagccgat ttaatcccct tacgtccttt ccgtatgagc cagcttcgcg ccggaaacag
      721 gcagaatatg cacataattt aatcgggaaa tcggtatgtt ttaagaattc attccggtaa
      781 acattagtca gagggacttc agccgttgga atcaaataga aattatcctg gggaacaaaa
      841 tacatctgac cttccttatc gggtaattgg ccagtagcaa aggccgaatc ctcattcacc
      901 aggagagggg gttcgtattc ggtataaccc gccttagtgg cttcatccag aaaaaaactg
      961 atcaagctac gctggagttt cgctcctttg tctttgtaaa atggaaaacc cgcaccagta
     1021 actttcgaac caatttcgaa actgatgatc tgatacttta cagctaaatc ccaatggcaa
     1081 agagcttcag ggggaagttg aggaatactg ccttccgaat atacgatttc attatcggcc
     1141 ggagaaatac caggcgatac tctctcatcg ggaatattgg gcaagcgaac caaaagttca
     1201 ttcatctgtc gtgaaatttc atcgagtctt tcctgcaggt tccttacctc ttcctttatt
     1261 tttgcaactt cattttttcg atcattggcc ttgccggctt gccctgcttt tatcaatgaa
     1321 ccgatttcct gagtcagttt tctatactca gccgatcttt catccgattc gaattgagtg
     1381 gattttctct tccaatcgag ttcgagtatc tgatagataa gactattggc gtcgaaattc
     1441 ttgaccgcca atctttgtat aacccagtcg ggttgttcac gaataatctg caagggaatc
     1501 attctgaata aaatttaaga ttgcaaaatt aggtctaaag cgataataaa aaactcccgg
     1561 ggttagaatg ccgggagcct tatcatgttt taactcaagt agatttagtt ttctttctta
     1621 ttgtcgggga aaaccgacac gtacgattta ttgtttttac gtcgatgaaa ctcgacaatt
     1681 ccatcggata gagcaaataa ggtatggtct ttacccaagc caacattaat ccccggatga
     1741 tgaattgttc ctctttgtct tacaataatc tgtccggctt tggcaaattg acctccgaaa
     1801 atctttacac ccagtcgttt gctttccgat tctctgccat tttgagaact tccggcacct
     1861 ttcttatgag ccatataaat tgaatattta aaagttaagc aacactaatt tcttcaatta
     1921 taattttaga taagggctga cgatgcccat tcagcttttg atatcctttc ctacgtttct
     1981 tcttaaatat aagcacttta tccgatttta tatgctcaga gatcgtcgcc tttactcggg
     2041 ctccttccac tcttggcctg ccaactctta tggtcgaatc atcatcaatc aacaatatat
     2101 tttcgaagat aacctgagtt gcttcatcac cttgcaaccg attgacataa tattcacgct
     2161 tggtttcaac ctttatttgt tgcccggcta tttctacaat tgcgtacatt ttatctcgat
     2221 taaaatttct tactcgtctc aatttttgaa agtgcaaaat tacaaaattt ctttcatcca
     2281 taattcatct aaaaaaattt tatctgttca gacacctttt cgaagttttg gttgtcttaa
     2341 gaaataagat ttagggatta taaccgggag gttaacccta attttggtta aaca
//
LOCUS       DAOORU010000222         2351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_55512_length_2351_cov_3.563153, whole genome shotgun sequence.
ACCESSION   DAOORU010000222 DAOORU010000000
VERSION     DAOORU010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55512_length_2351_cov_3.563153"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            453..>2351
                     /gene="pulA"
                     /locus_tag="PLF20_09900"
     CDS             453..>2351
                     /gene="pulA"
                     /locus_tag="PLF20_09900"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR02104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HOX81350.1"
                     /translation="MGVKNFIFRNKTIYMLGLLSIITGFEPQQETHYKTDNYDQFPYY
                     EGNDLEMTYSQEGTFFRVWAPTAEQVQVRIYKDYDDKECLMAVNMEQDVGGTWKKYVE
                     GDFHHRYYTFRIQYQGDWLDETPGIYVKATGVNGKRGFICNPSEANPEGWQDDKGPIL
                     TSSVDAVIYELHLRDISIHPNSGIKNRGKYLGLIEKGTKNIWGQSTGLDHLKELGITH
                     VHLLPVADFLSIDESKTNSRQYNWGYDPQNYNVPEGSYATNPYDPLNRIGELKQMVKA
                     LHEAGIGVIMDVVYNHTGEIQKSNFNLIVPGYYYRHTAKGEWSNASGCGNETASERPM
                     MRRFIVESVKYWASEYHIDGFRFDLMGIHDIETMNLVRQTLYEINPNIIIYGEGWTAG
                     DSPFPAERRALKTNIAQMPGIAVFSDELRDGVKGPWYNSTESGFIGGVTGLEESIKFG
                     IVAATKHPQIDYQKVNYSKAPYAAQPDQCINYVSCHDNHTLWDKIRLTVKNASEEELV
                     KMNMTASAIVLTSQGIPFLHNGIEMMRSKKGVENSFNSPDSINQIDWNWKYLYSRAVK
                     FHQQLIELRKQHPAFRMKTSNEISENLHFLSIPKPCIIAYTLNGKAVGDSWRKILVIF
                     NGNRHPENI"
BASE COUNT          791 a          431 c          477 g          652 t
ORIGIN      
        1 gtggtcaggc aacggtttcc tatctcccac gtcctacacc taaacccata aaacacgatt
       61 gattcgatta aaacattatt taacacagaa caagttctca acctacttta taccgatttt
      121 aggtttatta tcaaatttta caaaagttgt gagccattgc tataaatttt cttatcaagc
      181 caatgaaact cgtgtttaag tgattcttga actatatatc ccttataacg agatcaatgt
      241 ttatatagct ttcacaataa aaaacctctc ttattggaaa aacttttctg taacttttaa
      301 taaaacaatt caaatctcga ttataattga atttcttgtt caatagagct ttattttaaa
      361 aaggaacgac atatttgcaa taagaaattt tggatataaa tgccaatgca ttagatttgc
      421 taaacaattt caaccctaaa ccatttgctg atatgggtgt taaaaatttc atattcagaa
      481 acaaaaccat ttacatgctt ggacttttga gtataataac cggatttgaa cctcaacaag
      541 agacccacta taaaacggac aattatgatc aatttcctta ttatgaaggg aatgatttag
      601 agatgactta tagtcaggaa ggaacatttt tcagggtatg ggcgcctacg gccgaacaag
      661 tgcaggtaag aatctataaa gattatgatg acaaggaatg tctgatggca gtgaatatgg
      721 aacaagatgt tggagggact tggaaaaaat atgttgaagg agattttcat catcgatatt
      781 atacttttcg tattcaatat caaggtgact ggctggatga gacgcctggt atttatgtaa
      841 aggcaacagg tgtaaacggc aagcggggtt tcatttgcaa tccatcggaa gccaatccag
      901 aaggatggca agatgataag gggccaatat tgacatcttc ggtagatgcc gtgatttatg
      961 agctacattt gcgagacata agtattcatc ccaattcggg gataaaaaac cgcgggaaat
     1021 atcttggcct tattgaaaaa gggactaaaa atatatgggg gcaatccaca ggtttggatc
     1081 acttgaaaga attaggtatc acacacgttc atttgcttcc ggttgccgat tttctttcga
     1141 ttgacgaaag caaaacaaac tcgcgacaat acaactgggg ttacgatcct cagaattata
     1201 atgttccaga aggttcttat gctacaaatc catatgatcc cttgaaccgc atcggtgaat
     1261 tgaagcaaat ggtgaaagcc ttgcatgagg caggcattgg ggttatcatg gatgtggttt
     1321 ataatcacac gggagaaata caaaaatcga acttcaacct gatagttccg ggatattact
     1381 atcgccacac ggccaaggga gaatggtcaa acgcaagtgg gtgcggcaat gaaactgcca
     1441 gcgaaagacc catgatgcgt cgttttatcg ttgaatcggt aaaatattgg gctagcgaat
     1501 atcacatcga tggctttcgg ttcgatttga tgggcatcca tgacatagaa accatgaatt
     1561 tggtcagaca aacattatat gaaatcaatc caaatattat tatttatgga gaaggatgga
     1621 cggctggtga tagtcctttc ccagccgaaa gaagagcact gaagacaaat attgcacaaa
     1681 tgccagggat agcggttttt tcggatgaat tgcgtgatgg tgtaaaagga ccatggtaca
     1741 atagtacgga atccggtttt ataggcggag taaccggact ggaagaaagt atcaagtttg
     1801 gtattgtggc ggctacaaaa cacccccaaa tagactatca gaaggtaaat tattccaaag
     1861 ctccttatgc ggctcaaccc gatcagtgta tcaattatgt atcttgccac gataatcata
     1921 cactctggga taaaatacgc ttaacggtaa aaaatgcttc cgaagaagaa ctcgtaaaaa
     1981 tgaacatgac tgccagtgcc atagtgctca cttcacaggg aatacccttt ttgcataatg
     2041 gcatagaaat gatgcgttcg aaaaaaggag ttgaaaactc atttaattcg cccgatagca
     2101 tcaaccaaat cgactggaac tggaaatatc tttacagccg tgcagtaaaa ttccatcaac
     2161 agctcatcga acttagaaaa cagcatcctg catttagaat gaaaacctcg aatgaaatct
     2221 cggaaaatct ccatttcttg tccattccga aaccatgcat aatagcttat accttgaatg
     2281 gaaaagcagt aggcgatagt tggcgtaaaa ttctggtcat cttcaatgga aatcgccatc
     2341 ccgaaaatat t
//
LOCUS       DAOORU010000223         2347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_55649_length_2347_cov_4.797993, whole genome shotgun sequence.
ACCESSION   DAOORU010000223 DAOORU010000000
VERSION     DAOORU010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55649_length_2347_cov_4.797993"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /gene="eno"
                     /locus_tag="PLF20_09905"
     CDS             complement(<1..582)
                     /gene="eno"
                     /locus_tag="PLF20_09905"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016387099.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOX81351.1"
                     /translation="MSNIAFISARQILDSRGNPTVEVDVITDDGVLSRAAVPSGASTG
                     IHEAVELRDEDPSYYLGKSVLKAVENVNTVLRDELKGMYIFDQNEIDARMIEIDGTPN
                     KGKIGANAILGVSLAVAKAAAIEANLPLFHYLGGPNANTLPIPMMNILNGGSHADNSI
                     DFQEFMIMPVGAMSFNDALRMGSEVFHHLAKVLK"
     gene            complement(663..1151)
                     /gene="rplQ"
                     /locus_tag="PLF20_09910"
     CDS             complement(663..1151)
                     /gene="rplQ"
                     /locus_tag="PLF20_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOX81352.1"
                     /translation="MRHGKKINHLGRTSAHRKSMQANMATSLIKHKRIVTTLAKAKAL
                     RTYVEPLINRSKLDTTHNRRVVFSYLQDKEAVTELFRVISPKIMNRPGGYTRILKLGF
                     RKGDGAEIAMMELVDFNELMLTGKTPKTGKTQRTRRGGRKRGTTGTSDEPKTPKKKET
                     IK"
     gene            complement(1156..2151)
                     /locus_tag="PLF20_09915"
     CDS             complement(1156..2151)
                     /locus_tag="PLF20_09915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOX81353.1"
                     /translation="MAILAFQRPDKVIMLQASDTKGVFEFRPLEPGFGITIGNALRRI
                     LLSSLEGYAITSIRIEGVDHEFSSIPGIVEDVTDIILNLKQIRFKYRGDNNQEGEDVH
                     VFIGGQETFTAGDINKFLSSFQVLNPDVVICNMEKSVKLNINLTIRKGRGYVPAEENK
                     MLNAPLGTISMDSIHTPIRNVSYQVENFRVEQKTDYEKLLLTIVTDGSIHPKDALREA
                     AHILIQHFLLFSDDKITLDPEMKAAAEEFDENIQQMRELLKTKLNDLHLSVRALNCLK
                     AADVETLGDLVSYTKNDLLKFRNFGRKSLQELEELIASKGLEFGMKVDKYKLDKD"
     gene            complement(2195..>2347)
                     /locus_tag="PLF20_09920"
     CDS             complement(2195..>2347)
                     /locus_tag="PLF20_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOX81354.1"
                     /translation="ITDSLSHNTRPYPWLEWDLNLMSGKFMNYPNREDIPENIKEQLI
                     VELYSK"
BASE COUNT          623 a          485 c          494 g          745 t
ORIGIN      
        1 cttcagaact ttggccagat gatgaaagac ttcggagccc atgcggagtg catcattaaa
       61 actcatcgcc cccaccggca tgatcatgaa ttcctgaaaa tcgatggaat tatcggcatg
      121 agaacctcca tttagaatat tcatcatggg gatagggagg gtatttgcat tcgggccacc
      181 gagataatgg aaaaggggga gattggcctc gatggcggca gccttcgcca cagccagtga
      241 aacacccaga atggcatttg ctcctatctt tcctttgttg ggcgttccat ctatttcgat
      301 cattcgggca tcgatttcat tctgatcgaa aatgtacatt cctttcaatt catcgcgtaa
      361 aacagtattc acattttcga ccgccttcag aacacttttg ccaagataat aagaaggatc
      421 ctcatcgcgc agttcgacag cctcatgaat tccagtagat gctcctgaag ggacggcagc
      481 tcggcttaaa actccatcat cggtaatcac atctacctca acagttggat tccctctcga
      541 atcgaggatt tgacgagcac tgataaaggc aatattactc attgttttta atttatttta
      601 tgtttgaata ccctaaaaaa ttcacaaaga tggagatatc tggcatttaa tttcgatcgg
      661 ttttacttaa tggtttcctt ttttttcgga gtttttggct cgtcagatgt gccggtagta
      721 cctcttttgc ggcctcctcg gcgtgtacgc tgagttttgc cggtttttgg agtttttcct
      781 gtcaacatca attcgttgaa atcaaccagt tccatcatgg caatttctgc tccatcgccc
      841 tttctgaaac ctaatttcaa aatacgggta taacctcctg gtcggttcat aatttttggg
      901 gagataaccc ggaagagttc agtaacggct tccttatcct gaagataact aaagacaact
      961 cgacggttat gagtagtatc cagtttggag cggttgatca agggttcgac ataagtgcgg
     1021 agtgcctttg ccttagccag ggtagtaact atccgcttat gctttatgag cgaagtagcc
     1081 atattagctt gcatggattt acgatgggca cttgttcggc caaggtgatt aatttttttc
     1141 ccgtgtctca ttttattaat ccttatctaa tttatactta tctactttca tcccgaattc
     1201 gagaccttta ctggcgatga gttcttcgag ttcctggagg gatttgcggc cgaagttcct
     1261 aaatttcagc aagtcgtttt tggtgtaaga caccagatcg ccaagagttt caacatcggc
     1321 agctttgagg caattaagag ctctaactga aagatgaagg tcgttgagtt tagttttaag
     1381 tagttccctc atttgttgaa tattttcgtc gaattcttca gctgccgcct tcatttcggg
     1441 atcaagggta attttatcgt cggagaaaag aagaaaatgt tgaatgagga tatgagccgc
     1501 ttcacgaagg gcatctttgg gatgaattga accatccgta acaatagtca gtaggagctt
     1561 ttcatagtcg gttttttgtt ctacccggaa attttcaacc tgatagctca cgttccttat
     1621 gggtgtatgg atagaatcca ttgaaatagt tcctaatgga gcattgagca ttttattttc
     1681 ctcggctggg acataacctc ttccttttcg gatggtcaaa ttaatattca attttaccga
     1741 tttttccatg ttacatataa caacatcggg attcaatacc tggaatgaag ataggaattt
     1801 attaatatca ccggcagtaa aagtttcttg accgccaata aatacatgaa catcttcgcc
     1861 ttcttggtta ttgtctcccc gatatttaaa gcgtatctgc ttaagattaa gaatgatatc
     1921 ggtaacatct tcaactatgc ccgggatcga tgaaaattcg tgatctacac cttctattct
     1981 tatggaagtg attgcatatc cttcgaggga gctcaataat atccggcgca gggcattccc
     2041 gatagtgatg ccgaaaccag gctccagcgg tctgaattca aacactcctt tggtatcgga
     2101 ggcttgaagc atgattacct tatcgggtct ttgaaatgct aatattgcca tagagtggtt
     2161 gttatctgat ttttaaaagt aaacataaaa aaaactactt ggagtatagc tccacgatca
     2221 attgttcttt gatattttcg ggaatatctt cacggttggg ataattcatg aatttcccgc
     2281 tcatcaaatt caaatcccat tccaaccatg gatagggtcg tgtattatgt gaaaggctgt
     2341 cggtaat
//
LOCUS       DAOORU010000224         2304 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_57201_length_2304_cov_4.107159, whole genome shotgun sequence.
ACCESSION   DAOORU010000224 DAOORU010000000
VERSION     DAOORU010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57201_length_2304_cov_4.107159"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /gene="lepB"
                     /locus_tag="PLF20_09925"
     CDS             <1..844
                     /gene="lepB"
                     /locus_tag="PLF20_09925"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOX81355.1"
                     /translation="QNEDYYRIVRMAEEEFKMRMGSRYREGMGREAVWHTYHVVARPV
                     DKRENYIKRCIALPGDTIQIINRQVYLNGKELPDPPLLQFNYLINTEGRGLSRRVLER
                     MDISKEDMGWFQQYAILPLTKDKVQQISRIPGVIEVKPQLAPAGEWSPDIFPFDSAYP
                     WNVDNFGPLYIPKQGNEVTLTLHNLPLYKRIIEVYEKNRLVVKGNKIFINGEEANSYR
                     FQQNYYWMMGDNRHNSADSRYWGFVPEDHIVGKAVFVWLSLDKDKSLTEGKIRWKKLF
                     RIPR"
     gene            complement(1215..1412)
                     /locus_tag="PLF20_09930"
     CDS             complement(1215..1412)
                     /locus_tag="PLF20_09930"
                     /inference="COORDINATES: protein motif:HMM:NF037084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81356.1"
                     /translation="MSSTSENSKTHMNRLLIEVFSRKHILFEKELRALYEIRFENCLK
                     MVQIEGRYWGRAFNHIVPIAI"
     gene            1945..>2304
                     /locus_tag="PLF20_09935"
     CDS             1945..>2304
                     /locus_tag="PLF20_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81357.1"
                     /translation="MKKNYKHGRHLMLKRMFSIAFLSLMAFHSFSQGIPDLLWEKVYP
                     GNGTDITRQLISTNDGGYAIISESDSYGPGIISAYLIKCDSLGNSEWSKCYGGSTFDF
                     PRGIIQTTDNGFLIATYS"
BASE COUNT          708 a          402 c          467 g          727 t
ORIGIN      
        1 tcaaaacgaa gattattatc gtattgtgag gatggccgag gaagaattca aaatgcgcat
       61 gggaagtcgt tacagggaag ggatgggcag agaagcggta tggcatactt atcatgtagt
      121 tgctcgtccg gtcgataaac gtgaaaatta tatcaaacgc tgtattgctt tacctggtga
      181 tacgatacaa attatcaaca ggcaagttta tctcaatggg aaagaattac ctgacccacc
      241 cttgttgcaa ttcaattatt tgataaatac tgagggacgt ggcttgagtc ggcgagttct
      301 cgagcgcatg gatatttcga aagaagacat gggatggttt caacaatatg ccatactgcc
      361 ccttaccaaa gataaagttc aacaaattag cagaatacct ggtgtaatcg aagtaaaacc
      421 gcaattggct cctgctggcg aatggtcacc cgacatcttc cccttcgatt cggcttatcc
      481 atggaatgtc gataatttcg ggcctttgta cattccgaaa caaggcaatg aggtcaccct
      541 taccctccat aatctacctt tatacaagcg aatcatcgaa gtgtacgaaa aaaaccggct
      601 agttgttaaa ggaaataaaa tttttatcaa tggagaagag gctaattcgt atcgttttca
      661 gcagaattac tattggatga tgggtgataa ccgacataat tcggctgatt cccgttattg
      721 gggctttgtt cccgaggatc acatagtagg gaaagccgta tttgtttggc tttctctcga
      781 taaagataaa tcgctcaccg aaggaaaaat taggtggaaa aagttatttc gcatccccag
      841 ataaggagtg tttcgatcaa aaggtttgag ttggaatact ctcttaggta attacgatat
      901 cgttaagaac tctcgacatt tgggtaaagc tctggtcatc atctaacgac atttcgagct
      961 tgtgtgtatg atagcggatt tgttagaaat aaggttatag aagattttta gcaggcattg
     1021 aatacttctt cgaccaactc cgaattattg gtcttcttcc gtttctctgc tatgacatct
     1081 accagcgttt tactccgggc tgcctagttt gagatatatt aatggcctga acttgatttt
     1141 ttatcagatc agtgaatgtt gtcgtattga tgattttctt taagcttcgc atgttatcaa
     1201 gcggtactat gatattatat agcaatagga acaatgtggt tgaaagcccg accccagtat
     1261 ctgccttcga tttgcaccat tttgaggcaa ttttcgaagc gtatttcata aagagcacgc
     1321 aactcttttt caaaaagtat atgcttacgg ctgaaaactt cgatcaaaag acgattcatg
     1381 tgagttttcg aattttcgga ggttgacgag atatggtttg ggttacagct tggcctcttc
     1441 gatccattgc tgattataac tattttttcg gatcgaagct ttcagagcct tgaccagctt
     1501 tatataaaaa atttacattc ggaaattgag aaacctgagc ttgtaaattc tgattacaat
     1561 agaataatga cagattttga gcaaaatcaa atgcaaacat tcttccttct gctttatttg
     1621 ttgatgagat ggacagagtt tttgacaagg aaggatttac gcttggctat aatagatata
     1681 gggaagaatg atgaaaattt cgattaattt ttaatccata cagattcaac ttttatttct
     1741 ggcctccacc aaaacaaggg ggatataatg tcacatccaa aagatactcg aatacacaaa
     1801 taactttcca tcagtttttt acattttata gagtaataat gcagtagtga aacatttact
     1861 ttgccgtatt attattgtac ctctaactga tttcttatca aatgattgag atatttttgc
     1921 tacaaattta aacagacaaa cactatgaaa aaaaattaca aacacggtcg gcatttgatg
     1981 ttgaaacgaa tgtttagcat tgcttttttg agtttgatgg catttcattc cttttcacag
     2041 ggaatacccg atttattatg ggaaaaagtt tatccaggca atgggacaga cattacaagg
     2101 cagcttatat ctactaatga tgggggatat gccattatta gtgaaagtga ttcttatggt
     2161 ccaggaatca tttctgctta tttaataaaa tgtgactcac ttggtaatag cgaatggagt
     2221 aaatgttatg gtggatcaac ctttgacttc ccaagaggaa taatacaaac aactgataat
     2281 ggcttcttaa tcgctaccta ttct
//
LOCUS       DAOORU010000225         2272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_58386_length_2272_cov_5.392873, whole genome shotgun sequence.
ACCESSION   DAOORU010000225 DAOORU010000000
VERSION     DAOORU010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58386_length_2272_cov_5.392873"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /gene="pyrH"
                     /locus_tag="PLF20_09940"
     CDS             <1..366
                     /gene="pyrH"
                     /locus_tag="PLF20_09940"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859761.1"
                     /note="Catalyzes the phosphorylation of UMP to UDP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOX81358.1"
                     /translation="AYLEAGKVVIISGGTGNPYFTTDTASALRGIEVKADILMKGTRV
                     DGVYTADPEKSPNAVKYSRLTFQEAYSKGLKILDLTAFSLCMENNLPIYVFNMDVKGN
                     LIRLIKGEHIGTLISNNFQ"
     gene            372..1754
                     /gene="hisS"
                     /locus_tag="PLF20_09945"
     CDS             372..1754
                     /gene="hisS"
                     /locus_tag="PLF20_09945"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOX81359.1"
                     /translation="MNQFPPSIPKGTRDFGPVEMHRRDFIFSTIRQVFQLYGFQPIET
                     PAMENLQTLLGKYGEEGDKLLFRILNSGNYLQNVPIECLHSGEPQKILPFIAEKGLRY
                     DLTVPFARYVVQHHDEITFPFKRYQIQPVWRADRPQKGRYREFYQCDADVIGSKSLLN
                     EGEFIKIIDEVFSRLNIKVSIRLNNRKILAGIAEYIGHGKQLIDITTSIDKLDKIGLD
                     EVDNELSKRGISNKAIEKLHPLLFLIGENEEKLNELQSYFTDNHLGIEGINEMKSVLE
                     YASAFEVEAKVIFDQSLARGLNYYTGTIIEVTSLDVNIGSICGGGRYDDLTGIFGLPN
                     VSGVGISFGADRIYDVMEQTGAFPHQASISTQILFVNFGSHEAGFCIRLISNLRANNI
                     SAELFPETSKLKKQLAYANAKSIPYVAIVGEDELNTSTITLKDMTSGTQEKLTVERFF
                     EKMLKMLSKD"
     gene            complement(1766..>2272)
                     /locus_tag="PLF20_09950"
     CDS             complement(1766..>2272)
                     /locus_tag="PLF20_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOX81360.1"
                     /translation="TLDTGRYKAFIANALDVSPADVHGLLLGGHGDNMVPLPRFTSLN
                     GIPITDLLDKEVLDRIVERTRKGGGELVNLMGTSAWFAPGAAAAQMVEAIVDDQKRIF
                     PVSAYLEGEYGLDDVFLGVPVKLSCNGIEEIIEIKLNQEEKAMLHESARSVKELMNVL
                     DEMKIFEE"
BASE COUNT          688 a          517 c          447 g          620 t
ORIGIN      
        1 gcttacctcg aagcaggtaa agttgtaatt atcagcggag gtactggaaa cccatacttt
       61 actaccgata cggcttcagc acttagagga atcgaagtca aagccgatat cctcatgaaa
      121 ggcacacgtg tggatggagt ttataccgcc gatcccgaaa aaagccctaa tgcggtaaaa
      181 tattcaaggc ttacctttca ggaagcatat agcaaaggcc tcaaaatact cgatcttacc
      241 gccttcagtc tttgcatgga aaacaacctc cctatctatg tcttcaacat ggatgtgaaa
      301 ggcaacctga ttcgactcat caagggcgaa catatcggaa cccttatttc caataatttt
      361 cagtaaattc catgaatcaa tttcctccat ccatacctaa agggactcgt gattttggcc
      421 ctgtagaaat gcacaggcgc gatttcatct ttagtaccat tcgtcaagtt tttcagcttt
      481 acggttttca gcccatcgaa acaccagcta tggaaaacct ccaaaccctt ctgggtaaat
      541 atggagaaga aggcgataaa ctactgttcc gaatcctcaa ttcaggaaat tatcttcaga
      601 acgtacctat cgagtgtttg cattcgggcg aacctcagaa aattctcccc ttcatcgccg
      661 aaaaaggatt gcgttatgac ctcaccgtgc cttttgcacg ttatgtggtt cagcaccacg
      721 atgaaattac cttcccattt aaaagatatc aaattcaacc cgtttggcga gccgacaggc
      781 ctcaaaaagg aagatacaga gaattctacc aatgcgatgc cgatgtgatt ggaagcaaat
      841 ctttgctcaa cgaaggcgaa ttcatcaaaa tcatcgatga agttttttca cgattaaata
      901 tcaaggtttc catacggctg aacaaccgta aaattttggc tggcattgct gaatatatag
      961 gccatggtaa gcaactgatc gacattacca cttccataga taagctcgac aaaattgggc
     1021 ttgatgaagt tgacaacgaa ctttcaaaac gtggcatatc caacaaggct attgaaaagc
     1081 tccatcctct gctttttctc ataggagaaa atgaggaaaa actgaatgaa cttcaatcat
     1141 attttactga taatcatctg ggtatcgagg gtatcaacga aatgaaatct gttttagaat
     1201 atgcctcggc ttttgaagta gaagccaaag tgattttcga ccaaagcctt gctcgcggac
     1261 tgaattacta cacaggaaca attatcgagg tcacttcgct cgatgtaaac ataggaagta
     1321 tttgtggagg tgggcgttac gacgatttga ccggaatttt cggccttcct aatgtttcgg
     1381 gagtaggtat ctcatttggt gccgaccgca tttacgacgt gatggaacaa actggtgctt
     1441 tccctcacca ggcttcaata tctactcaaa ttctcttcgt aaattttggt tctcatgaag
     1501 ctgggttttg tatccgcctt atttcgaatc ttcgagcaaa taacataagt gccgagctat
     1561 tccccgaaac ctctaaactt aaaaagcaac tggcttatgc caatgcaaaa tctatcccct
     1621 atgttgccat tgtaggtgaa gacgaattga atacctctac aataacgctg aaagatatga
     1681 cttcgggaac tcaagaaaag ctgacggtcg aaagattctt cgaaaaaatg ttaaaaatgt
     1741 tgtcgaaaga ctaagaactc aatcgttatt cttcgaaaat cttcatctca tccagcacgt
     1801 tcattagttc ttttacggat cgggccgatt catgaagcat ggccttttct tcctgattaa
     1861 gcttgatttc gatgatttcc tctatgccgt tacagcttaa ttttacagga accccaagga
     1921 atacatcatc gaggccatat tcaccttcaa gataggcact aacggggaag atacgtttct
     1981 gatcatcgac gattgcctcg accatttggg cagcagctgc accgggtgca aaccaggccg
     2041 aagtacccat gaggttcacc aattcacccc ctcccttacg tgttcgctcg acaattctat
     2101 ctaatacttc cttatcgagt aaatcagtaa tgggaattcc attcaaggaa gtgaatcttg
     2161 gcaaaggaac catattatct ccatgaccac caagaagcaa accatgaaca tccgctggtg
     2221 aaacatcgag tgcattagca atgaaagctt tataacggcc tgtatcgaga gt
//
LOCUS       DAOORU010000226         2113 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_65042_length_2113_cov_7.382896, whole genome shotgun sequence.
ACCESSION   DAOORU010000226 DAOORU010000000
VERSION     DAOORU010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65042_length_2113_cov_7.382896"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            56..562
                     /locus_tag="PLF20_09955"
     CDS             56..562
                     /locus_tag="PLF20_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOX81361.1"
                     /translation="MKNNQEILSQIKAPVIIRLDSNINEIWAEVHTLDNCMTYLLSVM
                     ETSDKGAAAIGTTHCSNAQAFHIYFLKTDTLGLVMGEKEISLPKTEEAELSIYPNPVR
                     ENCHIHFGLGARQIQLFNLQGQVTREWLVPQGCTFMDVSLKNLPTGIYLFRVTGNEGV
                     KSMRVVKM"
     gene            916..>2113
                     /locus_tag="PLF20_09960"
     CDS             916..>2113
                     /locus_tag="PLF20_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOX81362.1"
                     /translation="MSDEEKMQIAAETYTADNIQVLEGLEAVRKRPAMYIGDVDVRGL
                     HHLVYEVIDNSIDEAMAGYCNDIKVIIHPDNSITVRDNGRGIPVDMHEKEKRSALEVV
                     MTVLHAGGKFDKDSYKVSGGLHGVGVSCVNALSSNLWVKIYREGKIWYQEYERGKPIE
                     PVKIIGESTMTGTETHFKPDPNIFNAIVFDVEILTSRLRELAFLNKKIRLSLIDERTS
                     EENGDYFTQEFYSDNGLIDFINYLDENREKLIPEPIYMEGERSGTPIEIVLQYSTSFA
                     ENIHSYVNNINTHEGGTHLAGFRRGLTRTLKTYAEKSGMLKNLKFDISGDDFREGLTA
                     IISVKVAEPQFEGQTKTKLGNSEVMGIVDQVVSETLNNYLEEHPKEARTIVNKVILAA
                     TARHAAR"
BASE COUNT          701 a          381 c          461 g          570 t
ORIGIN      
        1 aataagtggt atataaaaaa atgtttagga actttttgaa ttttgtcggt taatagtgaa
       61 aaataatcaa gaaattcttt ctcaaataaa agctccagtg atcatacggc ttgattccaa
      121 tattaatgaa atatgggctg aagttcacac tctcgataac tgcatgacat atttattaag
      181 tgtaatggaa acttccgaca aaggggctgc cgctatagga actacccact gctcaaatgc
      241 acaagcattt catatctatt ttctaaaaac cgataccctg ggtttggtta tgggggaaaa
      301 agaaatttcc cttcctaaaa ccgaagaagc agagctcagt atatatccca atccagttag
      361 agaaaattgc catattcatt ttggccttgg agcaaggcaa attcaactat tcaaccttca
      421 gggccaggta acaagagaat ggcttgtgcc ccaaggttgt accttcatgg atgtatcgtt
      481 gaaaaatctt ccaacaggga tttatctatt cagagtaact ggaaatgagg gagtgaaaag
      541 catgagagta gtaaaaatgt agataagctt aaacacgcgg ataggataaa ttggtaacta
      601 ttggaaacat gccaaaatat gggtaatttt tttaatttca atggctggga taagctggta
      661 ttttctttcc cacaaaaaca aatttaagaa ttaagccttt gcttgctgcc ctgcaggtct
      721 aaaatatttc ctcttttcat gatagaaact tataaacata cttagaacaa aatcgggcag
      781 atagccgatt cattgaaata aaatttaccg aacagcctta tcttttgaaa agatttattc
      841 ataaattttc gtcctgggta agagaaaaaa tagtatcttt gcaaacgtaa atgattgaaa
      901 aataattatt tgattatgag tgacgaagaa aaaatgcaga tagcagccga gacctatacg
      961 gccgacaaca ttcaggtatt ggaagggttg gaagcggtac gcaaaaggcc ggcgatgtac
     1021 attggcgatg ttgatgtaag aggattgcat catctggttt acgaagtgat tgacaattcg
     1081 atagacgaag ccatggctgg ctactgcaac gatattaagg tgatcattca cccagacaat
     1141 tccatcaccg ttagggataa tggcagagga atccccgtag atatgcatga aaaagaaaag
     1201 cgttcggcac ttgaagtggt catgacggtt ttacatgcgg gagggaaatt cgacaaggat
     1261 tcgtataaag tatcgggcgg cttgcatggc gtgggggtat cgtgtgtaaa tgctctttca
     1321 tcgaatcttt gggtaaaaat atatagagaa ggtaaaatct ggtatcagga atatgaaaga
     1381 ggcaaaccca tagaacctgt caaaattata ggagaaagta ccatgacagg gaccgaaacg
     1441 catttcaagc ccgatcccaa tatttttaat gccattgtct tcgatgtgga aattcttacc
     1501 agtcgtttgc gtgaactggc cttcttgaat aaaaaaattc gtttaagcct tatcgatgaa
     1561 agaacaagtg aagagaatgg agattacttt actcaggaat tctattcgga taatggcttg
     1621 atcgatttta tcaattacct cgacgaaaac cgtgagaaat tgattcccga gcctatttac
     1681 atggaaggcg aacgctctgg tactcctatt gagattgtac tacaatacag cacttctttt
     1741 gccgagaata ttcattctta tgtgaataac atcaatacgc acgaaggcgg aacgcatctg
     1801 gccggatttc gtcgcggcct cactcgaacc ctcaagacat atgccgaaaa atcgggcatg
     1861 ttgaaaaatt taaagttcga tatctcgggc gatgatttca gggaaggcct taccgccatt
     1921 atttcagtga aagtagccga acctcagttc gaaggacaaa ccaagactaa gttaggcaac
     1981 agcgaagtga tgggtatagt agatcaggta gtatctgaga cgcttaacaa ttatctggag
     2041 gaacatccca aggaagctcg taccatagta aacaaggtga tactggcagc tactgcgcgt
     2101 catgcagccc gca
//
LOCUS       DAOORU010000227         2069 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_67138_length_2069_cov_6.886296, whole genome shotgun sequence.
ACCESSION   DAOORU010000227 DAOORU010000000
VERSION     DAOORU010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67138_length_2069_cov_6.886296"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLF20_09965"
     CDS             <1..183
                     /locus_tag="PLF20_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HOX81363.1"
                     /translation="FEGHRWYDLKRTHRAIEVINNVKGENDIPLGYQLTENRLLWPIP
                     QAELDKNSKLTQNPGY"
     gene            258..1727
                     /locus_tag="PLF20_09970"
     CDS             258..1727
                     /locus_tag="PLF20_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 30 beta sandwich
                     domain-containing protein"
                     /protein_id="HOX81364.1"
                     /translation="MKTLIPVSSMIYSLLLLSALGLFSACGKNDNPQAEPPVDTTHYA
                     KAQVWLTKGDKTVLFSKQNGLNILTSVAPKWPTITVDTSFRFQEIEGYGAALTGSSAY
                     LFNRVLTPNARQSLIENLFTPDSGIGLSYLRLTIGASDFSLSDFTYDDVEPGQTDFNL
                     QNFSLSQDTLDVVPILKEILKVAPNIRLLGSPWSPPAWMKTNGSLKGGRLKQECYPVY
                     ARYFVKYIQSMQTNGIRIDAITPQNEPLYATASYPCMLMEAAHQKELIRDHLGPAFQQ
                     YGLDVKIIVYDHNWDRPDYPIEILNDQETRKYVAGSAFHAYAGDVSTMSFVHQAHPDK
                     AIYFTEISGGDWATNFSDNLLWNMKNILIGTANNWSKNALFWNLALDENHGPKNNGCS
                     NCRGVVTITSQGQIYKNEEYYSLAHFSKFIRPEAVRILSRIAQQLMNIDAVAFQNIDS
                     SKVLIVANYSDTYKTFTVIQNQKSFNYSIAPKSVATLIW"
     gene            complement(1819..2031)
                     /locus_tag="PLF20_09975"
     CDS             complement(1819..2031)
                     /locus_tag="PLF20_09975"
                     /inference="COORDINATES: protein motif:HMM:NF025668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HOX81365.1"
                     /translation="MSKSGELKGFAAVTLQPNESQNVKILLNPRAFSYYDQTQGKWVI
                     QSGKYKILTASSSTDIRQQASFEIMP"
BASE COUNT          598 a          458 c          391 g          622 t
ORIGIN      
        1 ttcgaaggtc atagatggta tgatctgaaa cgtacgcatc gtgccataga ggtcatcaat
       61 aatgtaaaag gcgaaaatga tattcccctt ggttatcagc ttaccgagaa ccgtttactt
      121 tggcctattc ctcaagctga acttgataag aactcaaaac tcacccaaaa tcctggttat
      181 tgatcttacg gggcgcaagc tttctttgaa agcttgcgcc tcttgctttt tcatctttgc
      241 ccagtccttt tattatcatg aaaactctga tccccgtttc ctcgatgatt tactctcttc
      301 tattgctatc agcacttggc cttttttctg cttgcggaaa aaatgacaac ccacaagctg
      361 aacctccggt tgatactaca cactatgcaa aagctcaggt atggcttaca aaaggggata
      421 aaaccgtttt attttcgaaa caaaatggtt tgaatatact cacctcggtt gcacccaagt
      481 ggcctacaat caccgttgat acttcttttc gatttcaaga aatcgaaggc tatggggcag
      541 ctctgacggg ttcatcggct tatctattta acagagtcct caccccgaat gctcgtcaaa
      601 gtttgatcga aaatttgttc actcccgatt caggtattgg cttatcctat ctgagactga
      661 caataggagc atccgatttt tcgctttccg attttactta tgatgatgtt gaacccggac
      721 aaacggattt taatttgcaa aacttctctc tttcgcaaga tactcttgat gtagttccta
      781 tattgaagga aattctgaag gttgctccaa acatccgatt attgggttcg ccttggagcc
      841 ctccggcttg gatgaaaacc aatggaagtt taaagggtgg acgcttaaag caggaatgct
      901 accccgttta tgcacgatat ttcgtaaaat atatacaatc catgcaaacc aatgggatac
      961 gcatagacgc cattactccc cagaatgaac ctttatatgc cacagcctct tatccttgca
     1021 tgcttatgga agctgcccac cagaaagagc tcattcgcga tcatttgggg ccagcattcc
     1081 aacaatacgg attggatgta aaaattattg tttatgacca taattgggat agacccgatt
     1141 atcccattga gattctcaat gatcaggaaa cacgcaaata tgttgctggc agcgcttttc
     1201 atgcttatgc tggcgatgtt tcaaccatga gtttcgtcca tcaagcccat cccgataagg
     1261 ccatctattt caccgaaatt tcaggtggag actgggctac caattttagc gacaacttgc
     1321 tctggaatat gaagaatata ctcattggca ccgcaaacaa ctggtcgaaa aatgccttgt
     1381 tttggaatct ggctctggat gaaaaccatg ggccaaaaaa caatggatgc tcaaactgca
     1441 gaggcgttgt tacaattact tctcaagggc agatttataa aaatgaagaa tattacagtc
     1501 tggcacattt ctccaaattc atacgcccag aggcagttcg cattctttcg cgcatcgctc
     1561 agcaattgat gaatattgat gcggtggctt ttcaaaatat agattcatcg aaagtgctca
     1621 tcgttgcaaa ttatagtgat acctacaaga catttactgt tattcaaaat caaaaatctt
     1681 tcaactattc catagcacca aaatcggtag caactctcat ctggtaattt cagaactatc
     1741 ctcacactat tctttgtata taaaaaaatc cagctttcgt tcattggaag ctggataatt
     1801 ttttcaattt tcaaagaatc agggcataat ttcgaaagaa gcctgctgac ggatatcagt
     1861 cgaggaacta gcagttagaa tcttgtattt tccactctgt atgacccact ttccttgggt
     1921 ttgatcataa taagagaagg cacgtggatt gagaagaatc tttacgtttt ggctttcgtt
     1981 gggttgaagc gtaacagccg caaatccttt aagctctcct gacttcgaca atgcgtcacc
     2041 ctaaaaattt ttccaaaact tttcatcaa
//
LOCUS       DAOORU010000228         1995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_70904_length_1995_cov_4.372165, whole genome shotgun sequence.
ACCESSION   DAOORU010000228 DAOORU010000000
VERSION     DAOORU010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70904_length_1995_cov_4.372165"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1995)
                     /gene="sprA"
                     /locus_tag="PLF20_09980"
     CDS             complement(<1..>1995)
                     /gene="sprA"
                     /locus_tag="PLF20_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HOX81366.1"
                     /translation="HTGYLTEGEVAGIPLIRVMGFDNLDPQLNPPPDGIFDFIDNAAT
                     QGGTIQSSNGRIYFPEVEPFGRDLRQKIGNEQLANKYCYDSLYTLTKVGAQQYPEKNK
                     FLLEGFYKSSVSSEISLNALNIPQGSVKVTHGGIPLTENVDYTVDYTIGRVKIINEAL
                     LSSGTPINISLESNTMFSIQSKTYLGTHIDYEISKDFILGATIINLSEKPLTQKTILG
                     EDPISNTIWGLNVAYQKESLWLTRLLDKLPLYSTSVPSKIAFTGEFAHFIPGHSSAIG
                     STGTSYIDDFEAAKSTIDLRLPNNWFLASTPQGQTAPGMFPEAALGTGLAYGFNRAAM
                     SWYIIDPIFYDRTGTVRPKNVTKDELSRNLVRQVLENEVFPAKEIPNGVPTNIAILNL
                     DFYPSERGPYNYDVTGVPGFSHGIDAEGFLRNPNSRWGGMMRKIESTDFEAANVEYIE
                     FWMMDPFSEDPDNPGGEIYFNLGDISEDILRDGKKSFENGLPVGPEVKDVDTTIWGRV
                     PKKQSLVNAFDNDPQSRQYQDVGYDGLSDEDERSFFDEVFLQQIVNLYGAGSQAYLKA
                     YEDPSSDNFRYFRGAAMDNDEQYKSVQARYKRYQGPDGNSPTAEQTGESYTAQSTSLP
                     NVEDINLDNTLSEDERYFQYRIDLRPDKMVVGENYITDIYE"
BASE COUNT          584 a          435 c          396 g          580 t
ORIGIN      
        1 ttcgtagata tcggtaatat agttctcccc aacgaccatt ttatcaggtc ttaaatctat
       61 ccgatattga aaatatcttt cgtcttctga cagggtattg tcgaggttaa tgtcttcgac
      121 attgggtagg ctggttgact gtgcggtata agattcaccg gtttgttcgg ccgtgggtga
      181 atttccatcc gggccttgat atcgtttata tcgtgcttga acactcttat actgttcatc
      241 attgtccata gcagcacctc taaaatatcg gaaattatcg gaggaaggat cctcataagc
      301 tttcaaataa gcctgagagc cagccccata aaggttgacg atttgttgta aaaatacctc
      361 atcgaaaaac gagcgttcat cctcatcaga aaggccatca tagccaacat cctgatattg
      421 acggctctgt ggatcattgt cgaaggcgtt gaccaaagat tgcttcttag gaacacgacc
      481 ccaaatagta gtgtctacat cttttacctc aggcccaaca ggaagcccat tttcgaaact
      541 cttcttacca tctctcaaaa tatcttccga aatatcgccc aaattgaaat aaatttcccc
      601 tccgggatta tcagggtctt cggagaaagg atccatcatc cagaattcaa tatattctac
      661 attagcagct tcgaaatcgg tagattctat ttttcgcatc attcctcccc aacggctgtt
      721 aggattgcgt aaaaaccctt cggcatctat gccgtgcgaa aaacccggaa ctccagttac
      781 atcataatta taaggtcctc gctccgaagg ataaaaatca agattcaaaa ttgcaatatt
      841 tgtaggtaca ccattgggaa tttccttggc gggaaaaact tcattttcga gtacctgcct
      901 gaccaaattg cgcgaaagtt catccttcgt aacatttttc ggcctaaccg ttcccgtacg
      961 atcgtaaaaa attggatcga taatatacca cgacatggct gcacgattga aaccataagc
     1021 caaacccgtt cctaatgcag cttcgggaaa cattccggga gcagtttgcc cctgtggagt
     1081 actggccaaa aaccagttgt taggcaaacg aagatcaatg gtcgatttag cagcttcgaa
     1141 atcatcgata taagaagttc ctgttgagcc aatggcactc gaatgccctg gaataaaatg
     1201 ggcaaactca ccagtaaagg ctatcttgga aggtacactg gtcgaatata acggaagctt
     1261 gtcgagtaaa cgcgtcaacc ataatgattc tttttgataa gccacattca aaccccatat
     1321 tgtattcgaa attgggtctt ctcccaggat agttttttgg gttaagggtt tttccgataa
     1381 attgataatt gtagctccaa gaatgaaatc tttactaatc tcataatcaa tatgagtacc
     1441 cagataagtt ttgctctgga tgctgaacat ggtgttgctt tcgagcgaga tgttgatggg
     1501 tgtacccgaa ctcaacaaag cttcattgat aatttttacc cgtccaatag tataatctac
     1561 ggtataatcg acattttcag tcaaaggaat accaccatga gttactttga ccgaaccttg
     1621 aggaatatta agtgcattaa gcgaaatttc actacttact gaggatttat aaaaaccctc
     1681 caaaaggaac ttattctttt cgggatattg ctgggctcca accttggtta aagtatatag
     1741 tgaatcataa caatacttgt tggccaactg ttcgtttcca attttttgac gaaggtcgcg
     1801 cccgaagggt tcaacttcgg gaaaataaat gcgcccattg ctcgattgga ttgttccacc
     1861 ttgagtagca gcattatcta taaaatcgaa aattccatcg ggaggaggat taagctgtgg
     1921 gtcgagattg tcgaaaccca ttacccgaat caaaggaatt cctgcaactt ctccttcggt
     1981 aagatatcct gtatg
//
LOCUS       DAOORU010000229         1930 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_74601_length_1930_cov_4.512533, whole genome shotgun sequence.
ACCESSION   DAOORU010000229 DAOORU010000000
VERSION     DAOORU010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74601_length_1930_cov_4.512533"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            439..1554
                     /locus_tag="PLF20_09985"
     CDS             439..1554
                     /locus_tag="PLF20_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009276039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HOX81367.1"
                     /translation="MEEKSPGIKSKRLASLDALRGFDMFWIMGGESLFIALGAATGWP
                     VLVWWAEQMEHVPWHGFHFYDMIFPLFLFISGISFPYSLAKRGDNKHGIYRHVIRRGL
                     ILVLLGIVYNNAIRFDFANMRYASVLGRIGLAWMFAALIYINTGFTFRAFWTVFLLLL
                     YWGLLAFFPAPDLGSSDPYSMEGSLTGYIDRLLCPGKLYLGILDPEGILGTVPAISTA
                     LLGMLTGSFVRSESFVKKTNQKVIFMIVAGVTLLIIGKIWDSVFPINKNLWSSSFVCW
                     TAGISLLLFAVFYFIIDVMEYKKWAFPLVVIGMNSITIYLAQRVFYLGYTSDFLFGGF
                     AALFNQTWQPVIIHLSYALTCWLLLYWLYKKKIFLKI"
     gene            complement(1739..>1930)
                     /locus_tag="PLF20_09990"
     CDS             complement(1739..>1930)
                     /locus_tag="PLF20_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81368.1"
                     /translation="LLKNAPHTAAMVVNSQWNYPYTREKAAFPLPGMETDKYFPPVSR
                     IDDAYGDRNLVCTYQTFDF"
BASE COUNT          516 a          319 c          413 g          682 t
ORIGIN      
        1 atattttttg ttgaaatagg agtaatccat ttctcaaaat tcgaatgatc ataatccaaa
       61 aatactatac gactgaatga attgatatat tccgatatcc atgattcata ttttattatt
      121 ttatacgtgt tttatgtgat tcttgaaaga ggagtatttc actttgtgaa gattattcat
      181 tttttgggcg gttaaaacag gaaaaatttt tcaagcatac caaatagttt taaaattcta
      241 taatatagag aaaatcaata taatatattc gatggtataa aatttatcca actaaagaaa
      301 agattaaaat gaaatggtct ttgcttttcg tagggtggag ttaaatattt cttaagagtt
      361 tgaatttatt tttcaggaac tttattgcat aaaccgcaat atttaggaat tttgaaacca
      421 aataaaccgt ttacatttat ggaagaaaaa tcaccaggaa tcaaatcaaa gcgtttggcc
      481 tctctggatg cattaagagg tttcgatatg ttctggatca tgggaggcga gtcgttattt
      541 attgcgttgg gggctgccac aggttggcct gtattagtat ggtgggccga gcagatggag
      601 catgtgccat ggcatggttt ccatttttac gatatgatct ttcctttgtt tctttttatt
      661 tcgggtattt cctttcctta ttcgctggct aaaaggggag acaacaagca tggtatttat
      721 cggcatgtga ttcgcagagg ccttatttta gttttgttgg gaattgttta caacaatgcc
      781 attcggttcg atttcgccaa catgcgctat gcaagtgtat taggtcgtat tggacttgca
      841 tggatgtttg ccgctcttat ttatataaac acaggattta cattcagggc attctggaca
      901 gtttttcttc tactgcttta ctgggggtta ttggcatttt tccctgctcc cgacttgggc
      961 tcttccgatc cttattcgat ggaaggaagt cttacaggat atatcgaccg attgttatgt
     1021 ccgggcaagc tttatctggg tattctcgac cctgaaggaa ttttgggcac agtgccagct
     1081 atctccactg ctttattagg gatgcttact ggttctttcg tccgttcgga atctttcgtg
     1141 aaaaaaacca accaaaaagt tattttcatg attgttgctg gtgtaactct attaatcatt
     1201 ggaaaaatct gggacagtgt cttccccatc aataaaaacc tgtggtcgag ttcatttgtt
     1261 tgctggacgg ctggcattag tcttttatta tttgcggttt tctatttcat catcgatgtg
     1321 atggaatata agaaatgggc tttcccattg gtggtcatcg gaatgaattc cattaccata
     1381 tatctggctc aaagagtatt ttatttggga tatacttccg attttctttt cggaggattt
     1441 gcagccctct tcaatcaaac atggcagcca gtcattatcc atctgtcgta tgcattgacc
     1501 tgttggcttc tattgtattg gttgtacaag aagaagattt ttttgaagat atgagtgtaa
     1561 catttcggcc ccggctattc catgagatat agaattatta ggcttttttg aatgatagga
     1621 tttttgcttg atgtcgcttg atgtcacata ataagaggta gtttcaaccc atcaaaatct
     1681 ttattctgta atatttgggt gggttgaaag ccttttgtag agaaattcag agggttcttt
     1741 agaagtcgaa agtctgatag gtgcaaacca agtttctatc tccataagca tcgtcgatgc
     1801 ggctcacagg ggggaaatat ttatcggttt ccattccggg caaggggaag gccgcctttt
     1861 cgcgggtata aggataattc cattgtgaat ttacaaccat ggccgcggta tggggagcat
     1921 ttttcaagag
//
LOCUS       DAOORU010000230         1927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_74796_length_1927_cov_3.674679, whole genome shotgun sequence.
ACCESSION   DAOORU010000230 DAOORU010000000
VERSION     DAOORU010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74796_length_1927_cov_3.674679"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..>1927
                     /locus_tag="PLF20_09995"
     CDS             89..>1927
                     /locus_tag="PLF20_09995"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOX81369.1"
                     /translation="MKRITICIALALLGFQIGNAQDVRLRMEKAITDPNKGYQLQVVD
                     AEDSEWSGSSKQVESILTPGINWHASDAASIGNVVKVSQTTLKTAVGWSLNYQRLSLY
                     GNSNIPEWEVPFTITAWDESVDMTDDGSRIANGYNDLVEVYLPTSSSPIWTTSIAPLS
                     VRGIQIQNDGQKVFVAGVNQSSADSSIVYCFQVGQPTPLWRKAYAGNYASLVISRSGN
                     RVLLGEYGGGINKVFVLNGMNGAEIFQSPISDQYPPAISEDGKFLVNGDYSGHVFLYE
                     YDDITQTYSERWTYQVNGSGSWVCGMGISADGSTLAVGTLMFTSTGYDGELYVFDTYS
                     PIPLWIYPNMGDMVQSIDLSADGSIIAAATWGPIGNATPDLYLFRKQSNTPYFTVNSN
                     GSLFCVDLSSDGKICVAGGKAVHAREFGMGGNVYNINSDLGGGTLTGMVLKSDLGQLG
                     GSKIEVVGLDTYFTYTDETGHYNLSSIPQGNYTVRYSAIGYITQDISDITINNGQTTT
                     QDVTLEPAGNPPTNLIATQGAGLQVDLSWEASTSPGVVGYNIYRKQYSFEYYPSTPLG
                     FVEAGKLSFADSTALPLIHYYYVVTAKLSGDLQTPYSNEAIGWISTG"
BASE COUNT          529 a          343 c          459 g          596 t
ORIGIN      
        1 tagaacaatc atggccttga tttttgttgt gcggcgtatg aaaattattt ttttttatgt
       61 aagtttgctt cgtaaaaatt ttaaacagat gaaaagaatt acaatttgca ttgctttagc
      121 actactgggg tttcagatag ggaatgctca ggacgtaaga ctaagaatgg agaaagcgat
      181 tactgatccc aataagggat atcagttaca agtcgttgat gctgaggatt cagaatggtc
      241 aggatcatct aaacaggtgg agtctatctt aactcctggg attaattggc atgcctcgga
      301 tgctgcgtct atcggtaatg ttgtaaaagt atcgcaaacc actctgaaaa cggcagtggg
      361 atggagcttg aattatcaac gtctttcgct atatggaaat tcgaatattc ccgaatggga
      421 agttcctttt accattacag cttgggatga atcggttgat atgaccgatg atggctctcg
      481 cattgctaat ggatacaacg atcttgtaga ggtttatctt cctacttcat cgagtcctat
      541 ctggacaacc agcattgctc cattatctgt aagagggatt cagattcaaa atgatgggca
      601 aaaggttttt gtagctggtg taaatcaatc ttcggcagat tcttcgatag tatattgttt
      661 ccaggttgga cagccaactc ctttatggcg aaaggcttat gctgggaact atgcttctct
      721 ggtgattagc cgttcgggaa accgtgtgct tctgggagaa tatggtgggg gaataaataa
      781 agttttcgta ctcaatggaa tgaatggtgc cgaaattttt caaagcccaa tatctgacca
      841 gtatcctcct gctatttcag aggatgggaa atttctggtg aacggagatt atagcggtca
      901 tgtttttctt tatgaatatg atgatattac gcaaacctat tcagagagat ggacttatca
      961 agtcaatggg agcggatcgt gggtatgtgg tatgggcata tcggccgatg gcagtactct
     1021 tgccgttggt acgctgatgt ttacttcgac gggttatgat ggtgaattgt atgttttcga
     1081 tacttattct ccgattccac tttggattta tcccaatatg ggggatatgg tgcaaagtat
     1141 cgatttgagt gccgatggtt cgataatagc tgctgcaact tggggcccta taggaaatgc
     1201 gacaccggat ttatatctct tcagaaaaca aagtaatacg ccttatttta cagtaaattc
     1261 gaatggttca cttttttgtg tagatctttc atctgatggt aaaatttgtg tagcgggtgg
     1321 taaggctgtt catgcccgtg aattcggcat gggtggaaac gtttacaata tcaattccga
     1381 tctgggcggt ggaacgctca ctggaatggt tttgaaatcc gatttgggtc aattgggtgg
     1441 atctaagata gaagtcgttg gcctcgatac ttattttacc tataccgatg agacagggca
     1501 ctacaatctt tcgagtattc ctcaaggaaa ctatacggtt cgctacagcg caatcggata
     1561 tattacgcag gatattagtg atatcacaat aaataatggc caaaccacca cacaggatgt
     1621 aacactcgaa ccagccggga atccgccaac taatctgatt gctactcagg gagcgggtct
     1681 tcaggtagat ttaagctggg aagcttctac atcgccagga gttgttggat ataatattta
     1741 caggaaacaa tattctttcg aatattaccc ctccacacca ttgggatttg tagaagcagg
     1801 gaaactctcg tttgccgata gtacggcatt gcctttgatt cattattact atgtggtaac
     1861 tgcaaaactt tcaggagatt tgcaaactcc ttattcgaat gaagctatag gttggatttc
     1921 aactggt
//
LOCUS       DAOORU010000231         1856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_79230_length_1856_cov_4.463631, whole genome shotgun sequence.
ACCESSION   DAOORU010000231 DAOORU010000000
VERSION     DAOORU010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79230_length_1856_cov_4.463631"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            283..1098
                     /locus_tag="PLF20_10000"
     CDS             283..1098
                     /locus_tag="PLF20_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HOX81370.1"
                     /translation="MKYESLLQYNPALLAFIATLFTWLMTALGASMVFFFKSINKKVL
                     NSMLGFAAGVMIAASFWSLLKPSIDMAEEMGMLAWVPAIIGFLAGGGLLFSIDRVLPH
                     LHMGLATSKAEGIKTSWQRSVLLVLAITLHNIPEGLAVGIAFGALTNNPEISTLMGGL
                     VLALGIGIQNFPEGAAVSIPLRREGFSRLKAFNYGQLSGLAEPIAGLIGVYLVLAITP
                     ILPYALSFAAGAMIYVVVEELIPESQIGDETDLSTVGTIIGFALMMLLDVAFG"
     gene            complement(1226..>1856)
                     /locus_tag="PLF20_10005"
     CDS             complement(1226..>1856)
                     /locus_tag="PLF20_10005"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HOX81371.1"
                     /translation="LTFEVDGQIYLNERATATQQTGFSFVSQMRSWLPREIGGIFWFS
                     VDDADNTVYYPVYCSSTRAADKYARGYGSMMEFRDDAAFWVFNQVSNFVYTRYRDIHP
                     EVRVMQDKLENFYIDFTPTIDSYALKLYGTDKSKAIDFLTDYSVNIGNNLVMQWKNFY
                     AYLFTKYMDGNIKVKDGNKKNPKVSQPGYGEQWYRYIVEKTGDKLKYKQ"
BASE COUNT          522 a          323 c          409 g          602 t
ORIGIN      
        1 acaggcagag gtgcacagtg aactaaggat ttgaagtagt ttttcatagt gtttctaaga
       61 aagtactttt gtaaaatatt gacgggtaat gataagatcg ggaattccga catttgagaa
      121 ggtcgaatcg gtactttttt tatatatcat accgtaaacc aattcattgt attttatcct
      181 ctttattgat tgacattaga atatcttcca taataaatag tttaggatta ttgtatggat
      241 gaagattctt agatttgaga aatattttaa ttgtttttgg gtatgaagta cgaaagtttg
      301 ttgcaataca atccagcttt acttgccttt atagcaacat tatttacatg gctgatgacg
      361 gcattgggtg cgtccatggt atttttcttc aaaagcatca ataagaaggt acttaattcc
      421 atgttgggat ttgctgccgg agtgatgata gcagcaagtt tctggtcgtt attaaagcca
      481 tctatcgaca tggctgaaga gatggggatg cttgcatggg tgcctgcgat aatcggattt
      541 ttagcaggag gaggtttatt attttccatc gacagggttt tgccccattt acacatggga
      601 ttagcaacca gtaaagctga agggataaag acttcgtggc agagaagtgt tttgctggtt
      661 ttagccatta cgcttcataa tattcccgaa gggcttgctg taggaatagc tttcggagct
      721 ttaaccaaca atcccgaaat aagtacacta atgggtgggc ttgttttagc tttaggtatt
      781 ggcattcaga attttccgga aggagcggca gtttctattc cactgaggag ggaaggtttt
      841 tcgagattaa aagcctttaa ctatggccag ctttctggtc ttgctgaacc catagccgga
      901 ttaatcggtg tctatttagt tttagctatc actcctattt tgccctatgc cttatcgttt
      961 gctgctggtg cgatgatcta tgtggtagtg gaagaattga ttcccgaatc gcaaattgga
     1021 gatgagaccg atctttcgac tgtaggtaca attataggtt ttgcgttgat gatgcttttg
     1081 gatgtagctt ttggataagg taaaagaagt aagatttagg aaattcatac aggatcggcc
     1141 ggagtggagg ataatgtagg aggtggccta cggctacctc ctacattatt tctaaagaca
     1201 attttatcga acaaaagcga atgatttatt gcttgtactt cagtttgtcg ccggtttttt
     1261 caacgatata acgataccat tgctcaccat agccaggttg actgactttg ggattcttct
     1321 tattgccatc ttttacttta atatttccat ccatatattt agtaaacaaa taggcataaa
     1381 aattcttcca ttgcattaca aggttattcc ctatattaac actgtaatcg gtgaggaaat
     1441 caatagcctt cgatttgtcg gttccgtata gtttcagggc ataagaatct atagtggggg
     1501 taaaatcaat atagaaattt tcgagcttat cctgcatgac ccttacttca gggtgtatat
     1561 cgcgataacg ggtatagacg aagttactta cttgattgaa tacccagaag gctgcatcat
     1621 ctctaaattc catcatactt ccatatccgc gggcatattt atcggctgca cgagtagacg
     1681 agcaataaac aggataataa acggtattgt cggcgtcgtc gacactgaac cagaatattc
     1741 caccgatctc acgaggaagc catgaacgca tttgcgaaac gaaagagaag cctgtctgct
     1801 gtgtcgcagt agctctttcg ttcaaataaa tttggccatc tacctcaaaa gttaat
//
LOCUS       DAOORU010000232         1846 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_79884_length_1846_cov_3.972641, whole genome shotgun sequence.
ACCESSION   DAOORU010000232 DAOORU010000000
VERSION     DAOORU010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79884_length_1846_cov_3.972641"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1846)
                     /locus_tag="PLF20_10010"
     CDS             complement(<1..>1846)
                     /locus_tag="PLF20_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81372.1"
                     /translation="KGLSIAYDGKNVYCATPYSIFYYNPDYNSLERLNKVNGLSDANI
                     VDIAYSNEYQTLVVAYLNTNLDLVKSGSVLNIGDIKRKPILGNKTINKVSLYGKFAYL
                     SCGFGIVVVDMVKEEINDTWYIGPDGSQINVYDLTADDTYFYAATQMGIYRAEKSNPF
                     LAYYGSWEKLSNIPVPNGAYNAIQYFDGYLLANNHNPTYIDDTVFVYDFSIEQWSRLN
                     VFNYPDCYRMGISEGKVILSFEGTLHVYDKGMKFLLASFNPSGLILHPRAGCFDKDGY
                     LWIADNSLGLVKTWGEGWHGEAYSPNGPFSANVYEMKMRENELWVASGGRKSTWENLY
                     MTDGLYNYSDGIWYNFNSSNVAAFDSIYDMVCLAIDPSNPKHIYAGSWSKGLMEFNNR
                     ELVTVYSKYNSPLEGFVADLNRVFISGLAFDSKNNLWVANSGASSILHCRKPDGSWQS
                     YNLGGNASGLDIGEMVIDDYDQKWILMRKEHSILVFNETLTQGKPYKILGSATGEGAI
                     PGNMVLSIAVDLDGEIWIGTDAGVAVFYNPDAVFSGYNFDAQRILVEQDGYVQYLLEN
                     ESVTAIAIDGANRKWIGTDKAGVFLLSTDGTQQIYHFTEDNSPLLSNSV"
BASE COUNT          574 a          427 c          317 g          528 t
ORIGIN      
        1 accgaattgg aaagcaatgg actgttatct tcggtaaaat gataaatctg ttgggtgcca
       61 tcggtcgaaa gcaagaaaac gcctgcttta tcggttccta tccatttgcg attggctcca
      121 tctatggcaa ttgcggtaac cgattcattc tcgagtaaat attgaacata gccatcctgt
      181 tcgaccaata tgcgctgggc atcgaaatta tagcccgaaa atacggcatc gggattgtaa
      241 aaaacagcta caccagcatc cgtaccaatc cagatttctc catcgaggtc aacagcaatg
      301 gaaagaacca tatttccagg aatagcacct tcgcctgtgg cggaacccaa aattttataa
      361 ggtttaccct gagtaagggt ttcattgaaa acgagtatag aatgttcctt acgcatcaat
      421 atccatttct ggtcgtaatc atctataacc atttcgccaa tatctaagcc gctggcattc
      481 cctcctaagt tataagactg ccaactacca tcgggcttcc ggcaatgcag tattgacgaa
      541 gctccactat tagcaaccca taggttattt ttactatcga aggccaagcc gctgataaaa
      601 acgcggttca gatcggctac aaatccttct aaaggactgt tgtatttgct gtaaacagta
      661 accaattctc tattattgaa ttccattaaa cctttactcc acgaacccgc ataaatgtgc
      721 ttcggattgg atggatcgat ggccaaacaa accatatcat atatgctatc aaaggcagct
      781 acatttgaac tgttgaaatt ataccatatc ccatccgagt aattatagag tccatctgtc
      841 atataaagat tttcccaagt actttttcgt cctccactgg ctacccaaag ttcattttct
      901 ctcattttca tttcatatac attggccgaa aaaggaccat tgggtgaata agcctcacca
      961 tgccatcctt caccccatgt cttcaccaat cctaaagaat tgtcggctat ccacaggtat
     1021 ccatccttgt cgaaacatcc ggcacgtgga tgcaatatca accccgaagg attaaacgaa
     1081 gccaacaaaa atttcatccc tttgtcatat acatgcaatg tcccttcgaa actcaatata
     1141 accttcccct ctgaaattcc catacgataa cagtcgggat aattaaaaac attaagcctc
     1201 gaccattgtt cgattgaaaa atcataaaca aaaaccgtat cgtctatata agtaggattg
     1261 tggttattgg ccaataaata accatcgaaa tactgaatag cattgtacgc accattggga
     1321 actggaatgt tagacagttt ttcccacgaa ccgtaataag ccaaaaaagg attgcttttt
     1381 tcggccctgt agatgcccat ctgggtggcc gcataaaaat aggtatcgtc ggcagttaaa
     1441 tcgtaaacat tgatttgcga accatcaggt ccaatatacc aggtatcatt gatctcttct
     1501 tttaccatat ctaccaccac tattccgaac ccacacgaaa gataggcaaa tttaccatac
     1561 agactaactt tattgatggt tttgttgcca agaataggtt ttcgtttaat atcaccaata
     1621 ttcagaacgc tgccactttt caccaaatcg agatttgtat tcaaataagc caccacaaga
     1681 gtttgatatt cgttgctata agcaatatcg acgatatttg cgtcggaaag cccattcact
     1741 ttattgagtc tttccaaact gttgtaatct gggttataat aaaaaatact atacggggta
     1801 gcacaataga catttttacc gtcataagcg atggataagc cttttt
//
LOCUS       DAOORU010000233         1746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_87170_length_1746_cov_10.562389, whole genome shotgun
            sequence.
ACCESSION   DAOORU010000233 DAOORU010000000
VERSION     DAOORU010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87170_length_1746_cov_10.562389"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..1591
                     /gene="nadB"
                     /locus_tag="PLF20_10015"
     CDS             23..1591
                     /gene="nadB"
                     /locus_tag="PLF20_10015"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOX81373.1"
                     /translation="MRVHTDFLVIGSGIAGLSFALKVAQHGKVLVVTKSCADEAATRY
                     AQGGIATVMYTPDTYEKHIRDTMIAGDELNDPEIVRITITESRDRIKELMEWGVNFDR
                     TEAGRFDLGREGGHSENRVLHHQDQTGLEIEKTLLQRVSENPNITIWENHFAIDLLTQ
                     HHLGQEVTRRTATIECYGAYVLDKENNEIKTVLAKVTMLATGGAGNVYSTTTNPPVAT
                     GDGIAMVYRAKGVIEDMEFIQFHPTALFNPPERPAFLISEALRGFGAELRNHKGEAFM
                     YKYDPRGPLAPRDIVARAIDNEMKIHGEEYVYLDCRHLPKDDLMNHFPTIYAKCLSIG
                     IDITHTMIPVAPAAHYLCGGIKTDKWGRSTINHLYAAGECARTGLHGANRLASNSLLE
                     ALVFSHRAAIDSIPFIEKVPFCDDIPDWNAEGMVLNEEMILITQTIRELQSIMSSYVG
                     IVRSNIRLQRALVRLKILYNETEDLYNRSILIPKICELRNLVNVAYLIIKAAMARKES
                     RGLHYTLDYPHPKK"
BASE COUNT          526 a          405 c          370 g          445 t
ORIGIN      
        1 taaatttgcg aaaaatatca tcatgcgtgt acatactgat tttttagtga ttggttctgg
       61 gattgcagga ttaagcttcg ccctgaaggt tgcacaacat gggaaagtac tggtcgttac
      121 gaaaagctgt gccgatgaag ctgcaacccg ctacgctcaa ggtggcatag cgacagtaat
      181 gtatactcct gacacttacg aaaaacatat tcgtgatacc atgatagcag gagacgaatt
      241 gaatgaccct gaaatagtgc gtataaccat cactgaatcg cgagatagaa taaaagaatt
      301 gatggaatgg ggggtaaact tcgaccgaac tgaagctggg cgcttcgact tgggacgtga
      361 aggtggacat tccgaaaacc gcgttcttca tcatcaagac caaaccggac tcgaaatcga
      421 aaaaacactg ctacaacgag taagcgaaaa tccaaatatt acaatctggg aaaatcattt
      481 tgccatcgat ttacttaccc agcatcatct tggacaggaa gtaacaaggc gtacagctac
      541 tattgaatgt tatggagctt atgtactcga taaggaaaac aacgaaatta aaacggtttt
      601 ggccaaagta acaatgttag ccacaggagg agccggcaat gtttacagca ctacgaccaa
      661 tcctccggtt gcaacgggtg atggtatagc catggtctat cgggcaaaag gggttatcga
      721 agatatggaa tttattcagt ttcatcctac cgccctgttt aatccacctg agcgacctgc
      781 cttcctcatt tcggaagcct tgaggggctt tggtgccgaa ttgcgaaacc ataagggtga
      841 agcattcatg tataaatatg atcctcgggg cccattggct cctcgcgata tcgtggccag
      901 agccattgat aacgaaatga aaattcatgg cgaagaatat gtttacctcg attgccgtca
      961 tttgcccaag gacgatctga tgaatcattt ccctactatt tatgccaaat gtcttagtat
     1021 tggcatagac atcacgcata ctatgattcc cgttgccccg gcagcgcatt acctctgcgg
     1081 tggaatcaaa accgacaaat ggggacgctc tacaattaat catctctatg ccgccggaga
     1141 atgtgccaga actggattac acggtgctaa ccgactggcc tcgaatagtt tgctcgaagc
     1201 cctcgtcttc tcccaccgtg ctgctatcga ttctatcccc ttcatcgaaa aagtcccctt
     1261 ttgcgatgat atccccgact ggaacgccga aggaatggtc ttgaacgaag aaatgatcct
     1321 cattacccaa actattcgcg aactccaaag catcatgagc agctatgtgg gcatagtgcg
     1381 ctcgaatatt cgcctgcagc gagcactggt cagacttaaa attctctaca acgaaaccga
     1441 agacctttac aaccgctcca ttcttatccc taaaatctgc gaattgcgta acctggtcaa
     1501 tgtggcttat cttatcatca aggcagctat ggccagaaag gaaagccgtg gcctccacta
     1561 taccctcgat tatccacatc ccaaaaaata aagcatagtg ttcatcactt aattgatacc
     1621 aaaattaaaa gaaagaatat ttctaccctg ctggaggttt gaaatggccg aatttaccct
     1681 aattccaatg gatttaaacg attcctattc tatgggaatt taacacctct aaaaatttcc
     1741 taaaat
//
LOCUS       DAOORU010000234         1604 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_100082_length_1604_cov_3.187218, whole genome shotgun
            sequence.
ACCESSION   DAOORU010000234 DAOORU010000000
VERSION     DAOORU010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100082_length_1604_cov_3.187218"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..>1604
                     /locus_tag="PLF20_10020"
     CDS             66..>1604
                     /locus_tag="PLF20_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81374.1"
                     /translation="MKFWGKIANLKSLFLLLSSFFSLMIQAEAEGTRELQPESNRNVR
                     LRLSYSLPVTNNDGGPFATPVAPPEYRLWVTIGNTSEIVYFGFKPESPNFSFVIKDST
                     GTVVYPASGTPVPLPTSGNGYISNYNQAVYGPKPINAAGYDPLTFSPNYAGNFYFEFS
                     WTPASGQRYITYWDITVVGSGNIEKKGRLWSKNWQLQMGNNNDPFTGVMYPYTNDQIT
                     TSIHFNGMAPARFTVACNPTGVSNTGNFIIDRKSVAGNYTYPEYKIFVNNPDSILFPT
                     GILGGVDSVVTSNQCDGHLNITLFVNKPGVANIILQINPAPGVQPEDINLTDSVYSGY
                     STTIVWNGLNGLGDSVPSGTIIEIEITYVNGLTHLPLYDVEYSASTWKGFIVDLIRPQ
                     GNKPKVFWDDSNLGGSINLSGCIDTKGCHSWGYSLGDENTINTWWFAVSTSLIPIELL
                     YKRSERYEHVYTICDGDSILIGSTYYKTAGTYYDSAINTLGCDSVHIHHLAVKPSPIV
                     DLGPD"
BASE COUNT          475 a          322 c          319 g          488 t
ORIGIN      
        1 gagaatttta agaactttta tcgaatctat catgtgtttt tctattacaa tctcgcattt
       61 gtactatgaa attttgggga aaaatcgcta atttaaaatc tttatttctt cttttatcga
      121 gttttttttc actcatgatt caagccgaag ccgaggggac aagagagcta caacccgaat
      181 ccaatcggaa tgtgcgactt cgattgagtt attcattacc agtgaccaac aatgatggag
      241 gtccttttgc aacacctgtt gctcctcccg aatataggct ttgggttact attggcaata
      301 catccgaaat agtctatttt ggtttcaaac ctgaaagccc caatttttct tttgttatta
      361 aggattcaac tggaacagta gtgtatcctg catcaggcac gcccgtgcct ttaccgactt
      421 ctggcaatgg atatatttca aattacaatc aagctgtgta tgggccaaaa cccattaacg
      481 cagccggata tgatccatta acattttcac ctaattatgc agggaatttt tatttcgagt
      541 tttcatggac cccggccagc gggcaaagat atattactta ctgggacatt actgttgtgg
      601 gaagtggtaa catcgaaaaa aagggcaggt tatggtcgaa gaattggcaa cttcaaatgg
      661 ggaacaataa tgatcctttt actggagtaa tgtatcccta tacaaatgac cagattacca
      721 cctccatcca tttcaatgga atggcacctg cacgttttac agttgcatgt aacccaacag
      781 gggtctccaa tacgggtaac tttatcatcg atcgaaaatc agtagcagga aactacacct
      841 atccagaata taaaatcttc gtcaataatc cagattctat tcttttccca acaggaatcc
      901 tgggcggtgt cgattcggtg gtaacatcca atcaatgcga tgggcatctg aatataacgc
      961 tttttgtcaa taaacccggg gtggccaata tcattctgca aattaatccg gcacccggtg
     1021 ttcagccaga agatatcaat cttactgatt cggtttacag tggatattca actactatag
     1081 tttggaatgg cttgaatgga ttgggcgatt cagttccaag tggaactatt attgaaattg
     1141 aaatcactta tgtaaacgga ttaacacatt tgccactata tgacgtagaa tatagtgctt
     1201 cgacatggaa aggctttatc gtagatttaa tacgacctca aggaaataaa cccaaagtct
     1261 tttgggatga tagcaacctt ggcggttcaa taaacctcag cggttgcatc gacactaagg
     1321 gctgccattc atggggatac agccttggcg atgagaatac tatcaataca tggtggtttg
     1381 cggttagtac ttcactcata cccatagaac ttctctataa acgctctgaa cgatatgaac
     1441 atgtatatac aatttgtgat ggcgatagta tattgattgg aagcacttat tataaaaccg
     1501 cagggactta ttatgatagt gccatcaaca ctttgggttg tgatagcgtt catattcacc
     1561 atcttgcagt taagccttca ccaatagttg atcttggacc tgat
//
LOCUS       DAOORU010000235         1435 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_120090_length_1435_cov_5.535507, whole genome shotgun
            sequence.
ACCESSION   DAOORU010000235 DAOORU010000000
VERSION     DAOORU010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120090_length_1435_cov_5.535507"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1226)
                     /locus_tag="PLF20_10025"
     CDS             complement(<1..1226)
                     /locus_tag="PLF20_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81375.1"
                     /translation="MMKWIVLFLPILFYNLHCEGQKKQAAVWIYGDYNKLDFNFSPMK
                     VSRTNASGWVGAASICDSNGVLQFFANGTSIINWQSLQLVNFEQQYPQYGPGYYGCNI
                     IIPFQSNPNLYQYFSVRGPLGNPARYRLFRFIVDMRLNGGSGGFDNSQTKEVKDWVGT
                     QLACLIHTNGKDTWLVARHWRSDSLVSLLVVDTGIVKAVYSKTGSPAAVPNIEGTGLV
                     STYDSQLKSSPNSELLLNPRRRKEFPYHELYRFNRDSGTLSKPLYIHDTIKASQFYIN
                     DFPDGAFSPDSRLLYVSTGTRNKPLNENDGPGHFWQYSLENYDSLAIAQSRIYLGLLG
                     RGVPSKPEPWPFSPKFQLGMDGKIYISPGIPYVDSLLSIIHCPNNRGSNCQLKFREIN
                     LKKGRNAAYFPTLNQT"
     gene            complement(1246..>1435)
                     /locus_tag="PLF20_10030"
     CDS             complement(1246..>1435)
                     /locus_tag="PLF20_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOX81376.1"
                     /translation="AYEFKVRSLASQRLRTRGRTMKAYSQLKENLFLDISDLPTGLYR
                     VKVISVNGLDTQALQIIK"
BASE COUNT          423 a          297 c          300 g          415 t
ORIGIN      
        1 aaggtttggt tgagggtggg gaagtaagct gcatttcgac cttttttgag attgatttca
       61 cgaaacttca attggcaatt actgccacga ttatttggac aatgaataat ggataataaa
      121 gaatctacat aaggaattcc aggactgatg taaattttac catccatacc cagttgaaat
      181 tttggagaaa atggccatgg ttctggttta ctcggcacac cacgccccag taagccaagg
      241 tatattcggc tttgagcgat agcaagggaa tcataattct caaggctata ttgccaaaag
      301 tggcccggac catcgttttc gttaagaggt ttgtttcggg ttccggtact tacatataat
      361 aatcgggagt ctggtgaaaa tgctccatcg ggaaaatcat tgatataaaa ttgagaggct
      421 tttattgtat catggatata aagtggcttc gacaaagttc cagaatctcg gttaaaccgg
      481 taaagttcgt ggtaagggaa ttcctttcta cggcggggat tgagaagcaa ttcactgtta
      541 ggagaagatt tcagctgcga atcgtaggta gaaaccaagc cagtgccttc aatatttgga
      601 acagcagcag gagagcctgt tttactgtaa actgctttta ctatcccggt atccactacc
      661 aataacgata ctaagctatc cgatcgccaa tgccttgcca ctaaccaagt gtctttgcca
      721 ttggtatgaa ttagacacgc tagctgagtt cctacccaat ctttcacttc tttagtttga
      781 ctattatcaa atcctccaga cccaccattt agccgcatgt ctactataaa tcggaataga
      841 cggtagcgtg caggattacc cagagggccc ctgacactga aatactggta aagattcgga
      901 ttggactgaa atggtatgat gatgttacaa ccatagtaac ctggaccata ttgagggtac
      961 tgttgttcga aatttaccaa ttgcaaactc tgccaattaa taatggaagt accattggcg
     1021 aaaaactgaa gtactccatt ggaatcacaa attgaagctg cccctaccca cccacttgca
     1081 ttagtcctgc ttaccttcat tggtgagaag ttaaaatcca gcttattata atccccatag
     1141 atccaaactg cggcttgctt cttttgtcct tcacaatgga gattgtaaaa aaggatggga
     1201 agaaaaagaa caatccattt catcatatca ccaatcagtt aggcgctact ttattatctg
     1261 taatgcctgg gtatcaagac catttacgct aataaccttt acccgataga gccctgttgg
     1321 caagtcggat atgtcaagaa ataagttttc ctttaactgg gaataggctt tcatcgttct
     1381 ccctcttgtg cgtagtcttt gcgaagcgag actacgcact ttgaattcgt aagca
//
LOCUS       DAOORU010000236         1417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_2537
            NODE_122609_length_1417_cov_5.231278, whole genome shotgun
            sequence.
ACCESSION   DAOORU010000236 DAOORU010000000
VERSION     DAOORU010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864612
            Sequence Read Archive: SRR4244858
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 13:13:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,007
            CDSs (total)                      :: 1,963
            Genes (coding)                    :: 1,950
            CDSs (with protein)               :: 1,950
            Genes (RNA)                       :: 44
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 3 of 13
            Pseudo Genes (incomplete)         :: 10 of 13
            Pseudo Genes (internal stop)      :: 2 of 13
            Pseudo Genes (multiple problems)  :: 2 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_122609_length_1417_cov_5.231278"
                     /isolate="AMR_MDS_2537"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="41.12 N 87.64 W"
                     /collection_date="2015-02-09"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="PLF20_10035"
     CDS             <1..685
                     /locus_tag="PLF20_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOX81377.1"
                     /translation="PVMAELFLVEGDSAGGTAKQGRDRRFQAILPLRGKILNVEKAMQ
                     HKIFDSEEIKNIFTALGVSIGTDEDSKALNLEKLRYHKIIIMTDADVDGSHIATLILT
                     FFFRYMKELIENGYVYIATPPLYLIKKGNIERYCWTEEERINIVAELSKDGSDKGIHI
                     QRYKGLGEMNAEQLWSTTMDPERRILRQVKIENGTEADRIFSMLMGDDVAPRREFIER
                     NARYANIDA"
BASE COUNT          454 a          278 c          275 g          410 t
ORIGIN      
        1 ccctgttatg gccgaactct tcctcgttga gggtgattcg gccggcggaa ccgccaaaca
       61 aggccgcgac cgccgcttcc aagctatact gcctcttaga ggtaaaatcc ttaacgtcga
      121 aaaagccatg caacataaaa tattcgacag cgaagaaatt aagaatattt ttaccgctct
      181 gggagtatct attggaaccg atgaagacag taaagccctg aatctcgaaa aacttcgcta
      241 ccacaaaatc atcatcatga ccgatgccga tgtggatggt agtcatatcg ctacacttat
      301 ccttaccttc ttcttccgtt atatgaaaga actcatcgaa aacggttacg tttacatcgc
      361 tactcctcct ctttatctca tcaaaaaggg aaatatagaa aggtactgct ggaccgagga
      421 agaacgaata aatattgtag ccgaactctc gaaggatggg tcggataagg gaattcatat
      481 acagcgttat aagggtttgg gtgaaatgaa tgccgagcaa ctctggagca ccactatgga
      541 ccccgaaagg cgtattttac gccaggttaa aatcgaaaat ggaactgaag ccgaccgcat
      601 tttctctatg ttgatgggtg atgatgtggc acccagaaga gaatttattg aacgtaacgc
      661 cagatatgct aacatagatg catgaggcgt attgaaaacg cattttatta atgcctctat
      721 tgcattgaca aatccctgcc ttcataactc cgaattgttt tatcaattca aaatcattgc
      781 tgtaaaaaac tcaactttaa tattttgccc gataaataaa ttaattgata aataccgaat
      841 atattagaaa atagtatagg gtctcgagat tttttaattg cttatcaata ttttcagaac
      901 tcttgatcct ataattgcat atcaatctcg tcataactga cgtgacaaag taccacctta
      961 aaaatctatt gggttttgtt taaatttcag tggcctaaga aacgataaaa gaagagtttc
     1021 aatttccttt gtggcttttg cacccctgcg tgtaatgttt ttctcgccaa gacgccaagg
     1081 actcgaagaa aaatcatttc aatcctttgt tcctttgtgg cctctgtgcc actgctgcaa
     1141 tgttttttcg ctaacgggct aagaaccttt aagattttaa ccataggaaa agaaatttaa
     1201 aaaattaatg attgcagatg gtagaagtat acctccatag gatgacagaa gtaattattt
     1261 agagcaggta atgctgaaac gctctcatca aaaccacgac aacctgatta aaacccaatc
     1321 aaaagttctg aagttgagaa cttgttctat gttaaataat atttttaatc gaatcaatca
     1381 tgttttatgg gtttaggtgt aggacgtggg agatagg
//
